LOCUS       DAOMTA010000001        14942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_9_length_14942_cov_2.715524, whole genome shotgun sequence.
ACCESSION   DAOMTA010000001 DAOMTA010000000
VERSION     DAOMTA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9_length_14942_cov_2.715524"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /gene="pssD"
                     /locus_tag="PLK68_00005"
     CDS             <1..476
                     /gene="pssD"
                     /locus_tag="PLK68_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HRM88821.1"
                     /translation="LKTRVIFTSSAGGHFSELCELSELMERYNSFLITEDHEMMKEYK
                     KTNKSRSWYMPAGTKEHLFKFLCNFPINIFKSFKAYLKVKPDVIIATGAHTTVPICYI
                     AKLFGKKVIFIETFANITTKTLSGKLVYPIADLFLVQWEEMLELYPKAKYRGGLK"
     gene            476..958
                     /gene="pssE"
                     /locus_tag="PLK68_00010"
     CDS             476..958
                     /gene="pssE"
                     /locus_tag="PLK68_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HRM88822.1"
                     /translation="MRIFVMFGTQDKRFDRLLNAILNSNFVNENEVYVQLGYTKGDYS
                     GINGQEYYTEDELNHQIEIADLIITHAGVGAIVSALKLKKRVIVVPRLGQYKEQNNDH
                     QVQIMERFDKQGYIIPCTDLSKLDETVNNAYNFEPKEYVADKQGIIDEITDFINCIKK
                     "
     gene            1048..1740
                     /locus_tag="PLK68_00015"
     CDS             1048..1740
                     /locus_tag="PLK68_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88823.1"
                     /translation="MLKKLLTVGLALTMMFAVTACSSKDSDSDKPNDNNTSDNTKQER
                     PFTESNGLIKYFTINDEKICLPETVGEYVKYLEKIGTKVELGDTGKSVDEAPKVDAGG
                     ISSMVAYLKVYLDDSDWQWFGIRYKNDTDKKQSVADCKVTQITLDYDTITEEENHYGI
                     KTTVFITKDDQEIPMNGKTTSSNLLKWIGSPQQNTDGHWHYTDDQGYKYEFVTENQKG
                     ILTRLAITYPTN"
     gene            2002..2883
                     /locus_tag="PLK68_00020"
     CDS             2002..2883
                     /locus_tag="PLK68_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88824.1"
                     /translation="MKKLIKILMVSMICLGLTACGEKKAAKAETTDDVAKIAEDNDLN
                     DEGFDNSGLFWKFSFAGMEFSVAFNVGDDPKFYYVTNTLTLANIDRIKINPDKDIGSQ
                     WIYLRPVNGEFVVDEEDIKTYNDKGRKEAYEAYQKKFEKLGLTSELLAKWTIIQFNQN
                     TRTDLIKNIQKDADTVLTKIKENGYNYEKDNKGRQIISSTEAYKIVISNKKCMVIDAA
                     FDLEAKTGYMYLPEQGTCGYSINGATQFIYQYSDNTFLKGEPTLEQYAEMKNIKNWYD
                     EFLNQFSTKTEILQLIK"
     gene            3067..3900
                     /locus_tag="PLK68_00025"
     CDS             3067..3900
                     /locus_tag="PLK68_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM88825.1"
                     /translation="MNSYAKIKEAQSSYTSTEKVIAKYILENAADVLKCSAQSLGEKT
                     GTSAAAIIRFSKKLGYNGFSELKMSLAQSKRAPEEKIDFIIDENDDIVTLADKCCRLN
                     MNTVLKTYQLIDTNQLDNAIKKLIAANTIYLFGVGGSAIVAQDVEQKLTRIGKKVIYN
                     KDLHVQLTFSESMNKEDAALFISYSGTTKGLVEIAKMIKNKNVPIISITQFKPNPLSK
                     LSDIILQVPNEEREIRMGAISSRISSLVMTDLLYYGVFKNDLTGNKDKLIETKRIVSK
                     I"
     gene            4008..>4424
                     /locus_tag="PLK68_00030"
     CDS             4008..>4424
                     /locus_tag="PLK68_00030"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRM88826.1"
                     /translation="MKLSQLDTEQINSNSLHIDEMTTEEILTVINQEDQKVAIAVKDV
                     IPQIAKAVDCIYEKMCMGGRLIYMGAGTSGRLGVLDASECPPTYGVDPTLVQGLIAGG
                     KEAFTTAIEGAEDSKEIAVDDLKKIEAIKQERYVKED"
     assembly_gap    4425..4434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4435..4961
                     /locus_tag="PLK68_00035"
     CDS             <4435..4961
                     /locus_tag="PLK68_00035"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRM88827.1"
                     /translation="LSPLDVVCGIAASGRTPYVVGALAYARKLGCSTISICCVKNGAI
                     SQHAHFPIEAVVGPEVITGSTRMKAGTAQKLILNMLSTSVMIKKGKVYKNLMVDVQPT
                     NEKLRIRAINIVQQAISVDEGTAKELLIEADNNVKIAILKGLTNMSKEECLKALKNSD
                     ENISQTIRTITKGE"
     gene            4966..5298
                     /locus_tag="PLK68_00040"
     CDS             4966..5298
                     /locus_tag="PLK68_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88828.1"
                     /translation="MNKYVVKNNTKKEYKNIFGKTDYDIPMFGFKSALFILMTTMLSL
                     LICINILNYLKIDFRIPNALISGIVCGFSVAYSQFFIERKKGVCKNFWIVGTIFSLIA
                     FMVIFMIK"
     gene            5311..6663
                     /locus_tag="PLK68_00045"
     CDS             5311..6663
                     /locus_tag="PLK68_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM88829.1"
                     /translation="MFTKISNLIEGKLAGPLEKLSNQRHLRAIRDGIIATLPLIIVSS
                     LLMVVAFSYNQMPADWGITQFIKNNAVAILLPYRMSMYIMTLYAVFGIGYSLAKSYNL
                     DGLSGAILAELTFLLTIVPVSMPDITESISALASKNAELNAFLQGLPSGYVIPAENLG
                     SAGMFIGIISAFIGVEIYRFTQVKGLKISMPAAVPPAVARSFESLTPTVIIILGVSTI
                     TMWLGINVHTIVGNLIKPLINATDTLPSTLLIIFLIMFFWSFGIHGDSIVSSLARPIW
                     LILLDQNTAAIAAGKAATHVGVEPFLQWFVHIGGSGATLGLAILFCVKAKSKYGKTLG
                     RTTILPSIFNINEPMIFGAPIVLNPMLLIPFIIIPLICATIAWIATSMHLVNCAVTIA
                     PWTLPGPIGAYLACGGDWRAAVLNIILIIISVIIYYPFFKMYDNELLAEEKSKEAVSA
                     "
     gene            6681..7559
                     /locus_tag="PLK68_00050"
     CDS             6681..7559
                     /locus_tag="PLK68_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRM88830.1"
                     /translation="MLYIGIDGGGTKTKMALFDDAGRKLKEIIKPSVHVLTQPREICI
                     QILKDGVMELDANFQAKVIAGLAGYGEQKEVRNKIATICKEAFGNREFSLYSDVRIAI
                     TGALGGGDGIVVVAGTGSIALSSKNNHITRCGGWGYQLGDEGSAYWIAKRMLALYCQQ
                     VDGRLEKTQLYYLIKEKCKLENDYDIITFINKLNHDRTSIASLAKLNGIAAKDNDKYA
                     LQIYKEAAYEIAVLIKTLAKNFTSPFKVSYIGGVFEYGEDYVLKPLNNYLQPLSCQLV
                     APLYSPEYGAYLLGKK"
     gene            7602..9047
                     /locus_tag="PLK68_00055"
     CDS             7602..9047
                     /locus_tag="PLK68_00055"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM88831.1"
                     /translation="MNKKRKILWGGATASSQYEGGYNLGGKGLDTQDCRPYLKRTSNA
                     TTSTRLLTQDIIDEAKSCKEVGNYPFRQGSDGYHHIDEDIQLLKELGIDIYRFSISWA
                     RLYPHGDEEMPNEEGIRFYDHIFKKVHQAGMKIFLTMNHYAVPLYLVETYGGWTNNIL
                     VDFYLRFAKTVFEHWGEYIDYYLPFNEINAGYFSPYNGVGLVKEKDRPYDQSLVFQSL
                     HHQFVASAKVIELGHKISSQSQSGCMVACFCYYPLTPRPEDNLKAVRDEQIHQWFAVD
                     VLANGYYPSYMNRFFQENKINIIITEEERKLLRDNTCDFVSFSYYSSAVATVEESSEQ
                     TAGNLVVSTKNPYLKASDWGWQIDPIGLRIMLNKMYDRTRKPVFISENGFGAHDKFEE
                     DGSIHDQYRIDYLNQHFQQIDEAIRDGVDVIGYIMWGIIDIVSAGSCEMEKRYGVIYV
                     DGDNEGKGSYQRYKKDSFAWYQNYIKQYKNN"
     gene            9194..10144
                     /locus_tag="PLK68_00060"
     CDS             9194..10144
                     /locus_tag="PLK68_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM88832.1"
                     /translation="MKKIKDYILAHKIKVTIIIIILLGIIGALGKEQPKLILKQNTVN
                     LEYGENFNYLDYVDKNKFDEEDISYSCDGFDEDNPAVGKYTITYKYGNSKKKLTLNIK
                     DTTAPQLIQTKEIDIIENDEIDYRDYLEIKELSKYELEIDDSGVSYLAAGNYNASIKA
                     SDKYGNTSTLIMPVSIKALELELSTTTLNLEENQNNTINIKTNSTQPVVYTSSDETIA
                     TVDQSGNIKALKSGTVKITAAIKNKSVTCNVTVKGKPVETSSIRTPVADDNISTTVYI
                     TKTGDCYHSSGCGYLSRSKIAISKSTAINTGYRPCSRCHP"
     gene            complement(10222..10656)
                     /locus_tag="PLK68_00065"
     CDS             complement(10222..10656)
                     /locus_tag="PLK68_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88833.1"
                     /translation="MKQFKKSLLIIGLCFLMIGCTNDAMSKVTKKLQDAGYDISYLTD
                     DFTAVNITKTEKDKDRIQFCAYLEKKVVTSISYIVLPADNSNIDKTIIGFIYVDKNDD
                     NIISESAQKEAKKILKKLDLSIDDLVNYALQVHEDKGKSLNS"
     gene            10777..11076
                     /locus_tag="PLK68_00070"
     CDS             10777..11076
                     /locus_tag="PLK68_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRM88834.1"
                     /translation="MDEDLIFQVLAIVSEIPWGKVASYKQIASLAGRPKNARLVGKIL
                     SHASMYGDYPCHRVVNSAGRLVPEWYEQRDLLLEEDVCFKDNGNVDMKKCKWQLD"
     gene            11256..11861
                     /locus_tag="PLK68_00075"
     CDS             11256..11861
                     /locus_tag="PLK68_00075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRM88835.1"
                     /translation="MNTVSVEKNAIEEVAKLVSDSRVVAFPTETVFGLGVKFGSHQAL
                     DALYELKHRDKGKAISMMISKAEDIEKYAYVDETAKKLIAAFMPGMITIILKKRPHID
                     DYFTASLDTIGIRIPDDPFVLSLLDKTGPMLVTSANLSGENSLVDDKAVMKVFHGKIP
                     LIVKGESISKKASTIVDLSKGKVEILRLGDISKEQIAEVIE"
     gene            11858..12301
                     /gene="rpiB"
                     /locus_tag="PLK68_00080"
     CDS             11858..12301
                     /gene="rpiB"
                     /locus_tag="PLK68_00080"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRM88836.1"
                     /translation="MKIAMACDHGGLRLKNVLKDYLEANGYIVEDFGTYSEDSCDYPD
                     YAGKAAKAVASGSCDRGVVVCGTGIGVSITANKVKGIRCALCHDVFSAKATRAHNDTN
                     MIAMGQRVIGEGLALEILTAWLEGKYEGGRHDQRIAKMMAYEGEE"
     gene            12303..13541
                     /gene="glyA"
                     /locus_tag="PLK68_00085"
     CDS             12303..13541
                     /gene="glyA"
                     /locus_tag="PLK68_00085"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRM88837.1"
                     /translation="MKDIAVFESVERELNRQRNNIELIASENFVSPEILELAGTVLTN
                     KYAEGYPGKRYYGGCKFVDEVETLAKERLCKIYGAEYANVQPHSGAQANTAVYMALLN
                     HGDKVLGMSLADGGHLTHGHPLNYSGINYEFHSYGVTKETETIDYEDFKKKCQEVKPK
                     LVVAGASAYSRTIDFEYMAKCAHEVGAMFMVDMAHIAGLVAAGLHPSPFPHADIVTTT
                     THKTLRGPRGGVIMCKEKYAADIDRAVFPGMQGGPLMHIIAAKAACFYEAMQPEFKKY
                     AAQVIKNAKALETALKEEGFRLVADGTDNHLILIDVKASCGISGKKAERLLDEINITA
                     NKNAIPFDTEKPFKASGIRVGTPAMTTKGFTEEDFREVGKIIAYRLKNEETDEIKEEC
                     LKRVRALTDKVEMYHDIKYI"
     gene            13555..14178
                     /gene="upp"
                     /locus_tag="PLK68_00090"
     CDS             13555..14178
                     /gene="upp"
                     /locus_tag="PLK68_00090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRM88838.1"
                     /translation="MATTILNHALIDHKLTIMRDKDTKTIVFKDNLDEIAMLMAYEVT
                     KELPLVDKEIVTPICPMIGKELKKQIILVPILRAGIGLVDGFRRIIPTAKIGHIGMAR
                     NEETLIPEEYYAKFPSGLEDSIVIIVDPMLATGGSASAAITNIKARGAKDIRLVCLVG
                     APEGVKVIEEDHPDVELILATLDEKLNEKGYIVPGLGDAGDRLFGTD"
     gene            14350..14556
                     /locus_tag="PLK68_00095"
     CDS             14350..14556
                     /locus_tag="PLK68_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRM88839.1"
                     /translation="MDQDKKKMLKDLFFCLQLGFQVIGAFLLAVIVGLRLDKYFNTHP
                     TILLILLFLAFGYVIKILLGAGKE"
     gene            14553..14906
                     /locus_tag="PLK68_00100"
     CDS             14553..14906
                     /locus_tag="PLK68_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRM88840.1"
                     /translation="MNEKDVLKQSVKVFIGGLIIFSIVGFVLKQISFPLGFILGYAVS
                     VLSFYIIIVMSDVILKMGQAIRFVVMMFIAKMILYIAGFMLAIKFDDIFNLISVFFGY
                     FVTKITINILGYIKR"
BASE COUNT         5439 a         1884 c         2868 g         4741 t
ORIGIN      
        1 atttaaaaac tagagtaatt tttacatcct ctgctggtgg gcattttagt gagttgtgtg
       61 aattaagtga actgatggag cggtacaact catttttgat tacggaagat catgagatga
      121 tgaaggagta caaaaaaact aataaatctc gttcttggta tatgccagca ggaactaagg
      181 agcatttatt taagtttttg tgtaatttcc ccataaatat ttttaaaagt tttaaggctt
      241 atttaaaagt taaaccagat gttattattg caacaggggc gcatacgact gtaccaatct
      301 gttacattgc gaaattattt ggcaaaaaag taatttttat cgaaacgttt gcgaatatca
      361 ctactaaaac tttatctgga aagcttgttt atccaattgc agatttattt ttggttcaat
      421 gggaagaaat gttggaatta tatccaaaag ctaaatatcg aggaggtcta aaataatgag
      481 aatatttgtc atgtttggaa ctcaagataa aagatttgat cgtctcttaa atgcaattct
      541 taactctaac tttgttaatg agaatgaagt gtatgttcag ctgggatata caaaaggtga
      601 ttattcagga attaatggtc aagagtacta tactgaagat gaattaaatc atcaaattga
      661 aattgcggat ttaattatta cccatgctgg agtaggggcg atagtatctg cacttaagct
      721 aaaaaaaaga gttattgttg tacctcgtct tggacaatat aaggaacaaa ataatgatca
      781 tcaagtacaa attatggaga ggtttgataa gcaggggtat attataccat gtactgattt
      841 aagtaagctt gacgaaactg ttaataatgc ttataatttc gaaccgaaag aatatgtagc
      901 agataagcag ggaattattg atgaaataac tgattttatc aattgtatta agaaataata
      961 agatttaaat ttaaaaaata gtagattaaa tcgaaaaaag tatttataat aaaatctgta
     1021 tatgtataaa taaaaaggag aaagtctatg ctaaaaaaat tattaacggt tggtttagca
     1081 ctaacaatga tgtttgctgt aacagcgtgt agttctaagg attctgattc agacaaacca
     1141 aatgataata atacttctga taatacgaaa caagagagac cttttacgga atcaaatgga
     1201 ttaattaagt actttactat taatgatgaa aagatatgtt tgcctgaaac agtcggcgag
     1261 tatgttaaat atttagaaaa aattggaact aaagtagaat tgggtgatac tggtaaatca
     1321 gttgatgagg cgcctaaggt agatgctggt ggtatttcat ctatggtagc ttacttaaaa
     1381 gtttatttag atgattctga ttggcaatgg tttggaattc gttataaaaa tgatacagat
     1441 aaaaagcaat cagttgcgga ctgtaaagta acgcaaatta ctttagatta tgatacaatt
     1501 acggaagaag agaatcatta cggtataaag acaacagtat ttattactaa ggatgaccaa
     1561 gaaataccaa tgaatgggaa aacaacaagc agtaatttgt taaaatggat tggtagcccg
     1621 caacaaaata cagatggaca ttggcattat acagatgacc aaggatacaa atatgagttt
     1681 gttacagaaa atcaaaaggg tattttaact agattagcaa taacatatcc aacaaattaa
     1741 ttcttatata agagacttaa ctagaaataa aggttcttac cctaaaccgg ataagaactg
     1801 aaataaatag taagtctctt ttttgcttta aataattata tctctcaaat tgattgaaca
     1861 aatattgtag catcgtttgt tttactaaat aaagcatgcc ataattatta tgtgattagt
     1921 agcagtaaaa ttttatcgat tggaagtgat agtaataaga ttaaaataaa ttcataagtt
     1981 aagcagggaa agggaaaaaa tatgaaaaaa cttataaaaa ttttaatggt atcaatgatc
     2041 tgcttgggtt taacagcttg tggtgaaaag aaagcagcta aagcagaaac tactgatgat
     2101 gttgctaaga ttgccgaaga taatgattta aatgatgaag gctttgacaa ttcaggtttg
     2161 ttttggaaat ttagttttgc tggaatggag tttagtgtgg catttaatgt tggagatgat
     2221 ccaaagttct actatgtaac taatacatta acgcttgcta atattgatcg tattaaaata
     2281 aatccagata aagatatagg gagtcaatgg atatatctac gaccagtaaa tggagaattt
     2341 gttgtagatg aagaagatat taaaacttat aatgataagg gaagaaaaga agcttatgaa
     2401 gcatatcaaa aaaaatttga aaaattggga ttaacttctg agttattagc taaatggaca
     2461 ataattcaat ttaaccaaaa tactcgaact gatttgataa aaaatataca aaaagatgct
     2521 gatacagtcc tcacgaaaat taaagaaaat ggctataatt atgaaaagga taataaggga
     2581 agacagataa taagttcaac cgaagcttat aagattgtca tttctaataa aaaatgtatg
     2641 gtaattgatg ctgctttcga tcttgaagca aaaactggat atatgtatct tccagaacaa
     2701 ggaacttgtg gttactcaat aaatggggca acacaattta tttatcagta ttctgataat
     2761 acattcttaa aaggcgagcc gacacttgaa caatatgctg aaatgaagaa tataaaaaat
     2821 tggtatgatg aatttttaaa ccaattctcc actaagacag aaatcttaca attaattaaa
     2881 taacctttat gtgtctaagt tatttaatca aaatattaca attagccatc tattttgtta
     2941 aatagatggt ttttatttga atatagtaaa atagaataaa ttaaaactaa gttatgaata
     3001 ctttgaaaaa tctagtaatt ttactagtgg attgatataa ttaattagaa aggggctaag
     3061 attattatga atagttatgc taaaataaaa gaggcacaaa gcagctatac atcaactgaa
     3121 aaggttattg ctaagtatat tttggaaaat gctgctgatg ttttaaagtg ttcagcgcaa
     3181 tctctggggg agaaaacggg tacctctgct gctgcaatta ttagattttc aaaaaaatta
     3241 ggttataacg gttttagtga attgaaaatg agtttagcgc agtctaaacg tgctcctgaa
     3301 gaaaaaatag attttattat tgatgaaaat gacgatatcg taactttagc tgataaatgt
     3361 tgtcgtttaa atatgaatac tgttttaaag acataccagt tgatcgatac taatcaatta
     3421 gacaatgcaa tcaaaaaatt gattgctgct aatacaatat atttatttgg tgtcggtggc
     3481 tcggcgattg tcgcacaaga tgtggaacaa aagttaacaa gaattggaaa aaaggtaatc
     3541 tataataaag acttacatgt tcaactaaca tttagtgaaa gtatgaataa agaggatgca
     3601 gcattattta ttagttattc gggtactaca aaaggacttg ttgaaattgc taaaatgata
     3661 aaaaacaaaa atgtaccaat tatatcaatt actcaattta agcccaatcc actttcgaaa
     3721 ttaagtgata ttattttaca agttcctaat gaagaaaggg aaataagaat gggagctatt
     3781 tcatcaagaa tctcatcact agtaatgaca gatttattat attatggtgt atttaaaaat
     3841 gatctaacag gaaataaaga taagcttatt gaaacaaaaa gaattgtttc taaaatatag
     3901 gcttttcttt tttttgttta taatgaaata atattttaaa taatttaaca tttatattga
     3961 aataatattt caatagatat ataataaata tggaaggagt ggaatggatg aaattaagtc
     4021 aattagatac agaacaaatt aacagcaatt ctttgcacat tgatgaaatg acaacagagg
     4081 aaattttaac ggttattaat caagaagatc aaaaagtagc aattgcggta aaggacgtca
     4141 taccacaaat tgcaaaagct gttgactgta tctatgaaaa aatgtgtatg ggtggtagat
     4201 tgatttatat gggtgccgga acatcaggac gtttaggggt gctagatgca tcggaatgcc
     4261 cgccaacata tggagttgat cctactcttg tccaaggtct aatcgctggt ggtaaggaag
     4321 catttacaac cgcaattgaa ggtgctgaag attctaaaga aatagcagtt gatgatttaa
     4381 aaaaaataga agccattaaa caagaacgtt atgtaaaaga agatnnnnnn nnnnaactta
     4441 gtccattgga tgttgtttgt ggaatagctg cttcgggaag aacaccatat gttgttggcg
     4501 ctctagcgta tgcgcgcaaa ctaggatgtt caacaatatc aatatgttgt gttaaaaacg
     4561 gagcaatatc tcaacatgcc catttcccaa tcgaagcagt tgttggccct gaagttatta
     4621 cagggtcgac gagaatgaag gctggtacag cacaaaaact aatccttaat atgttatcaa
     4681 catctgtaat gataaaaaaa ggaaaagttt ataaaaattt gatggttgat gtacagccaa
     4741 caaatgaaaa actaagaatt cgagctataa atattgttca gcaagctatt agtgttgatg
     4801 agggaactgc aaaagaatta ttaattgaag cagataacaa tgtaaaaata gcaattttaa
     4861 agggtctaac taatatgagt aaagaagaat gcctaaaagc attgaaaaat agtgatgaaa
     4921 atattagtca aactataaga acgataacta aaggagaata agtatatgaa taagtatgta
     4981 gtcaaaaaca ataccaaaaa agaatataaa aatatatttg gcaaaacaga ttatgatatc
     5041 ccaatgtttg gatttaaaag tgctttattc attctaatga cgactatgtt gtcattattg
     5101 atttgtataa acatattaaa ttatttaaaa atagattttc gtattcctaa tgctttaatt
     5161 tctggtattg tttgtggctt tagcgtagct tattcacaat tttttattga gcgaaaaaaa
     5221 ggagtatgca aaaacttttg gattgttgga actatattta gtttgattgc ttttatggtt
     5281 atattcatga ttaaatagga ggaaaataag atgtttacaa aaatatctaa tttgatagaa
     5341 ggaaaactag caggaccact tgaaaaactt tctaatcaaa gacatttacg ggctatccgt
     5401 gatggaatta tagcaacctt gccattaatt attgttagtt cattattaat ggttgtagca
     5461 ttttcatata atcaaatgcc cgcagattgg ggcattaccc aatttattaa aaataacgca
     5521 gtagcaattt tattgccata tagaatgtca atgtatatta tgacgctata tgcagtgttt
     5581 ggaataggtt attcacttgc taaatcatat aatttggatg gcttgagtgg tgcgatttta
     5641 gctgaattaa cttttttatt gacaattgta ccagtttcga tgccagatat tacagagtca
     5701 attagtgcat tagctagtaa aaatgccgaa ttaaatgctt ttttacaagg tttgccttca
     5761 ggctatgtta ttccagcaga aaatcttggt tcggcgggaa tgtttattgg aattatctcg
     5821 gcttttatcg gggtagaaat ttatcgtttt acacaagtta aaggtttgaa aatctcaatg
     5881 ccggcggcag taccccctgc agtagcaaga tcttttgaat cgttaactcc tacggttatt
     5941 attattttag gagtttctac aattacaatg tggttaggta tcaatgtgca tactatcgtt
     6001 ggtaacttaa tcaagccatt gataaatgca acagatacct taccatcaac attactaatc
     6061 atatttttaa ttatgttttt ctggtcattt ggtattcatg gagattcgat tgtttcttcg
     6121 ctagcaagac caatctggtt aattttatta gatcaaaata cagctgcaat tgcagctggt
     6181 aaagcggcta ctcatgttgg tgtagaacca ttcttacaat ggtttgttca tatcggtggt
     6241 tcaggagcaa cattgggatt agcaatacta ttctgtgtaa aagcaaaatc taaatatggt
     6301 aaaacattag gaagaacaac gattttaccg tcaatattta atatcaatga gccgatgatt
     6361 tttggggctc caatcgtttt aaacccaatg ctactaattc catttattat tattccgcta
     6421 atttgtgcaa ctattgcctg gattgcaact tctatgcatt tagtcaattg tgctgttaca
     6481 atagctccat ggacattacc aggaccaatt ggtgcatact tggcttgcgg tggtgactgg
     6541 cgagctgcgg tattaaatat tatcttaatt attatatcag tgattattta ttatccgttc
     6601 tttaaaatgt acgataatga attattagca gaggaaaaat ctaaagaagc agtaagtgca
     6661 tagaaaatag ggagttattt atgttgtata tcggtattga tggtggtgga actaaaacaa
     6721 agatggcact ctttgatgat gcaggaagaa aattaaaaga aataataaag ccatcagtac
     6781 atgttttaac acaaccaaga gaaatatgta ttcaaatttt aaaagatggt gtgatggaat
     6841 tagatgcaaa ttttcaagca aaagtgattg cagggctggc cggatatgga gaacaaaaag
     6901 aagttagaaa taaaattgcg actatttgta aggaagcatt tggaaatcgg gaattctcct
     6961 tatacagtga tgttaggatt gctattactg gagctttagg tggtggtgat gggattgtag
     7021 ttgtagctgg aacaggttca atcgcattat catctaaaaa taaccatatt actagatgtg
     7081 gaggatgggg ctaccagcta ggtgatgaag gaagtgctta ttggattgcc aagcgcatgt
     7141 tagccctcta ttgccaacaa gttgatggtc gactagaaaa gacacagtta tactatttaa
     7201 ttaaagaaaa atgtaaacta gaaaatgatt acgatattat cacttttatt aataaactta
     7261 atcatgatcg aacatcaatt gcttcattag caaaactaaa cggtatagca gctaaagata
     7321 atgataaata tgctttacaa atatataaag aggcagctta tgaaattgct gttctaatta
     7381 aaacattggc caaaaacttt acttctccct ttaaagtatc gtatattggt ggagtttttg
     7441 aatatggaga agattatgta ttaaagccat tgaataatta tctccagcca ctatcatgtc
     7501 agcttgttgc ccctttatac agtccagaat atggagcata cttgttaggt aaaaaataaa
     7561 ggtgattttt atcaccttta tttttagaag gaggaggaca aatgaataaa aaaagaaaaa
     7621 tactatgggg tggagctact gcaagtagtc aatatgaagg tggctataat ttgggtggta
     7681 aaggtttaga tactcaggac tgtcgacctt atttaaaaag aacgtctaat gctacaactt
     7741 caacaaggtt attgacacaa gatataattg atgaagcaaa atcatgtaaa gaagttggta
     7801 attatccatt ccgccaagga tcagatggct atcatcacat tgatgaagat attcagttat
     7861 tgaaagaatt aggaattgat atttatcgct tttctatcag ctgggcaaga ttgtatccac
     7921 atggtgatga agaaatgcca aatgaagaag gaatacgttt ttatgaccat atttttaaaa
     7981 aggttcatca agcaggaatg aagatttttc taaccatgaa tcattatgct gtaccattgt
     8041 atttagttga aacctatggt ggttggacaa acaatatctt agtcgatttt tacttaagat
     8101 tcgctaaaac tgtatttgaa cattggggag aatatattga ttattattta ccattcaatg
     8161 aaataaatgc tggttatttt agtccatata atggagttgg tttagtaaaa gagaaagatc
     8221 gaccatacga tcaaagtcta gtatttcaat cattgcatca tcaattcgtt gctagtgcta
     8281 aggttattga attagggcat aaaataagtt ctcaaagtca atctggctgt atggtagctt
     8341 gtttttgtta ctatccgtta acaccgcgcc cagaagacaa tcttaaagca gtgagagatg
     8401 aacaaattca tcaatggttc gcagtagatg ttttggctaa tggctattat cctagttata
     8461 tgaatcgttt ttttcaagaa aataagatta atatcataat aactgaggaa gaacgtaagc
     8521 tgctaagaga taatacctgt gattttgtaa gcttttcata ttattctagt gccgtcgcaa
     8581 cggttgaaga gagcagtgag caaacagcag gtaacttagt tgtttcaact aaaaatcctt
     8641 atttgaaagc aagtgactgg ggttggcaaa ttgatccaat tggattacga atcatgttaa
     8701 ataaaatgta tgatcgcacc cgcaagcccg tatttatttc tgaaaatggt tttggggctc
     8761 atgataagtt tgaagaagat ggctctattc atgatcaata tagaatagat tatctaaacc
     8821 agcatttcca acaaattgat gaagccatca gagatggtgt tgatgtcatt ggctatatta
     8881 tgtggggaat cattgatata gtctcagcag gcagttgtga aatggaaaaa cgttatggtg
     8941 taatttatgt tgatggtgat aatgagggta aaggaagtta tcaaagatat aagaaagata
     9001 gttttgcctg gtatcaaaat tatataaaac agtataaaaa taattaagat aagaaagata
     9061 ggttcgtttc attaatatcc tatcttttta tcttgtttta cgtaataaac cgtatcttta
     9121 cgacaaaaat atcgtgaatt ttcataagta atagtataat gattatgtga atttatggga
     9181 gagtaaagat aaaatgaaga aaataaaaga ctatatttta gctcataaga tcaaggtaac
     9241 gatcataatt attattttat taggtattat tggtgcattg gggaaagagc aaccgaaatt
     9301 aatacttaaa cagaatactg ttaatctaga atatggagaa aattttaatt atttagatta
     9361 tgttgataag aataaatttg atgaagaaga tatttcatat agctgtgatg gttttgatga
     9421 agataatcca gcagtaggta aatatactat cacttataaa tatggtaata gtaaaaagaa
     9481 attaacgctt aatatcaaag atacgacagc accgcaatta atccaaacaa aagaaattga
     9541 tattatcgaa aatgatgaaa tagattatcg tgattattta gaaataaaag aactatcaaa
     9601 atatgagctt gaaattgatg atagtggtgt aagttattta gcagctggaa attataatgc
     9661 tagtattaag gcaagtgata aatacggaaa tacatctacc ttgatcatgc cagtaagtat
     9721 taaggcatta gaattagagc tttcaactac tacattaaat ctagaagaaa atcaaaataa
     9781 tactataaat ataaaaacaa atagtactca accggtagtt tatacatcta gtgatgaaac
     9841 gattgcgaca gttgatcaaa gtggaaatat taaagcatta aaatcaggaa cggtaaaaat
     9901 aacagcagca attaaaaata aatcagttac atgtaatgtg accgtaaaag gaaaaccggt
     9961 agaaacatcc tctattcgaa cgcctgtggc agatgataat atttctacaa ctgtttatat
    10021 tactaagaca ggagactgct atcattcaag tggttgtggc tatttatcac gtagtaaaat
    10081 agctatctca aaatctactg caataaatac aggatatcgc ccttgcagta gatgtcatcc
    10141 atagtgtaaa atgatctcat gaaagtgaga tcgttttata ttttaaaaat aataaaagac
    10201 acttaaaaag tgtctttcag actatgaatt taaagatttt cctttatctt catggacttg
    10261 aagggcatag tttactagat catctataga taaatctaat ttttttaata tttttttagc
    10321 ttccttttgg gcactttcac taataatatt atcatcattt ttgtcgacat agataaaacc
    10381 aataatcgtt ttatcgatat tactattatc ggcaggtaag acaatatatg aaatagaagt
    10441 aacaaccttc ttttctaaat aagcacaaaa ttgaatgcgg tctttatcct tttctgtctt
    10501 agtaatattt acagctgtga aatcatcggt taaataagaa atatcatatc cagcatcttg
    10561 aagcttttta gttactttac tcatggcatc attagtgcag ccaatcatta aaaagcataa
    10621 tccgattatt aataaagatt ttttaaactg tttcataaat ctcctttccc aagctaataa
    10681 aataattata caaaaaagta taataaaata caaaaaaata aatttttttg cattttttcg
    10741 ataattagat aaaataaaaa tataggaggt gattatatgg atgaggattt aatttttcaa
    10801 gtattagcaa tcgtgtctga aataccttgg gggaaagttg ccagctataa acaaatagca
    10861 tcgttggcag gacgacccaa gaatgcgcga ttagtgggaa aaattttaag tcatgcttct
    10921 atgtatggag attatccatg tcatcgagtt gtaaatagcg caggaagact tgtacctgaa
    10981 tggtacgaac aacgtgattt acttttagaa gaagatgtat gttttaaaga taatggcaat
    11041 gttgatatga aaaaatgtaa atggcagtta gattagatct aattgttttt ttgtaagaaa
    11101 ttgtcttatc aaactataaa aatgaaggaa attagcaatg ttaagataat gtaaagatgt
    11161 tttttttaaa aaactttttt tgtcgtgaaa atatctacga aatatctaca aattttcaat
    11221 tatttattgt ataatggggc aaatgaggtg attccatgaa tacagtttca gtcgaaaaaa
    11281 atgccattga agaagtcgct aagttagtta gcgatagtcg agttgtggct tttccaactg
    11341 agactgtttt tgggttagga gttaagtttg gctcacatca ggcattggat gcactatatg
    11401 aattaaagca ccgcgataaa ggaaaagcaa tttctatgat gattagtaag gcagaggata
    11461 ttgaaaaata tgcttatgtt gatgaaactg caaaaaaact tattgcggct tttatgccgg
    11521 gaatgatcac tatcatttta aagaaacgcc cacatattga tgattatttt acggcttcac
    11581 tagatactat tggaatcagg ataccagatg atccatttgt tttatcatta ttagataaaa
    11641 caggaccaat gttagtaact agcgccaatc tttctggaga aaatagtctt gttgacgaca
    11701 aagcagtaat gaaagtattt catggaaaga ttcctttaat tgttaaaggt gaaagcatca
    11761 gtaagaaggc aagtacaatt gttgacttaa gtaagggtaa agtggaaata ttaagattag
    11821 gtgatatttc taaggaacaa atagcggagg taattgaatg aaaattgcaa tggcttgtga
    11881 ccatggtggc ttaagactaa aaaatgtttt aaaagattat ttagaagcta atggttacat
    11941 agtagaagat ttcggaacat acagcgaaga tagttgtgat tatcctgatt atgcaggaaa
    12001 agcagctaaa gcggttgcta gtggatcgtg cgatagaggg gttgtcgtat gtggaactgg
    12061 tatcggtgta agtattactg ctaataaagt gaaaggaatt cgctgtgcgt tatgccacga
    12121 tgtatttagc gccaaagcaa ctcgagcaca taatgatact aacatgattg ctatggggca
    12181 acgagttatt ggtgaaggtt tagctttaga aattttaaca gcctggttag aaggcaaata
    12241 tgaaggtggt cgacatgacc agcggattgc aaaaatgatg gcttacgagg gggaagagta
    12301 aaatgaaaga catcgcagta tttgaaagtg tagagagaga attaaatcgt caaagaaaca
    12361 atattgagtt aattgcttct gaaaactttg tttcaccaga aatcttagaa ttggcaggaa
    12421 ctgtattaac taataaatat gcagaaggtt atccaggaaa aagatattat ggtggatgta
    12481 aatttgttga tgaagtggaa acactagcta aagaaagact atgtaagatt tatggtgctg
    12541 agtatgctaa tgttcagcct catagtggag cgcaagcaaa cacagcggta tatatggcgt
    12601 tattaaatca tggtgataag gtattaggaa tgtctcttgc tgatggtgga catttaacac
    12661 atggacatcc attaaattat tcaggaatca attatgaatt ccacagttac ggggtaacta
    12721 aagaaactga aactattgat tatgaagatt ttaagaaaaa atgccaagag gtcaaaccaa
    12781 aattagtagt agctggtgct agtgcatatt ctagaacgat tgattttgaa tacatggcaa
    12841 aatgcgcgca tgaagttggg gcgatgttta tggttgatat ggcacatatc gcagggttag
    12901 tggcagcggg actgcatcca tcaccatttc ctcatgctga tattgtaaca acaacgactc
    12961 acaaaacttt acgtggacct cgtggtgggg taattatgtg taaggaaaaa tatgcagctg
    13021 atattgatcg tgcggtgttc ccgggaatgc aaggtggtcc attgatgcat attattgctg
    13081 ctaaagccgc ttgtttttat gaagcaatgc aaccagaatt taaaaaatat gcagctcaag
    13141 taattaaaaa tgctaaagca ttagaaacag cattaaaaga agagggattc agattagttg
    13201 ctgacggaac tgataatcat ctgattttaa ttgatgtaaa agcaagttgc ggtatttcag
    13261 gtaaaaaagc agaacgttta ttagatgaaa ttaatattac tgcaaataaa aatgctatcc
    13321 catttgatac agaaaaacca tttaaagcaa gtggtattcg ggttgggacc cctgcaatga
    13381 ctactaaagg atttacagaa gaagatttta gagaagtagg taagattatt gcttatcgct
    13441 taaaaaatga agaaactgat gaaatcaaag aagaatgctt aaagagagtt agagcattaa
    13501 ctgataaagt agaaatgtat catgatataa aatacattta aggagtgaat ttatatggca
    13561 acgaccatcc tcaatcatgc attaattgat cataaattaa caattatgcg tgataaagat
    13621 acgaaaacaa tcgtatttaa agataaccta gacgaaattg caatgttaat ggcttatgag
    13681 gtaactaaag aattaccatt agttgataag gaaatcgtca caccaatttg tccaatgatt
    13741 ggaaaagaat tgaaaaaaca aattattctt gttccaatct taagagcggg gattggttta
    13801 gtcgatggtt ttagaagaat tataccgact gctaagattg gtcatatagg aatggctcgg
    13861 aacgaggaaa ctttaattcc cgaagagtat tatgctaaat ttcctagtgg tttagaagat
    13921 tctatcgtaa tcattgttga tccgatgtta gcaactggtg gtagtgctag tgcagcgatt
    13981 acaaatatta aagcccgtgg tgctaaagat attcgtttag tctgtttagt aggtgcccct
    14041 gaaggtgtta aagtaattga agaagatcat cctgatgttg aattgatttt agcgacgctt
    14101 gatgaaaaac ttaatgaaaa aggctatatt gtgcctggtt taggtgatgc cggtgatcgt
    14161 ttatttggta ctgattaata tttgagatac ttcaaaagtg gttaaaacgt ctaattatga
    14221 aattaattat agtgtttagt actgaaatac ttgttctata aataaattat catagtgtta
    14281 aataattatt acaaattttt ataagaagtt tacataacca aaagactata gtagaataca
    14341 agagggatta tggatcaaga taagaagaaa atgttaaaag atttattctt ttgcctacaa
    14401 ttgggttttc aggtaattgg agcatttcta ttagctgtaa ttgttgggct gcgcttagat
    14461 aagtatttta atacccatcc cacgattcta ctaattttat tgtttttagc ctttggctac
    14521 gttattaaaa tacttttagg agcaggtaaa gaatgaacga aaaagatgtt ttaaaacaat
    14581 ccgtaaaagt atttattggc ggattaatta tcttttctat cgtaggattt gtcctaaaac
    14641 aaattagttt tccactagga tttatcttag gatatgcagt cagcgtttta tcattctata
    14701 ttatcattgt aatgagtgat gtgatcttaa aaatgggtca ggcgattcga tttgttgtaa
    14761 tgatgtttat agctaagatg atattgtata ttgcaggttt catgctagca atcaaatttg
    14821 atgatatctt taatttgatc agtgttttct ttggatattt tgtgaccaag attacgatca
    14881 atattttagg atatataaaa cgctaaatat tttaaggagg tgaaatgagt gacaattcaa
    14941 gt
//
LOCUS       DAOMTA010000002        14397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_12_length_14397_cov_2.515897, whole genome shotgun sequence.
ACCESSION   DAOMTA010000002 DAOMTA010000000
VERSION     DAOMTA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12_length_14397_cov_2.515897"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLK68_00105"
     CDS             complement(<1..308)
                     /locus_tag="PLK68_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM88841.1"
                     /translation="MICRNLTEYYNIFFNKLAFQYYQYDNFSLYINPQQPEEFIIHVH
                     IADMYELFVGDFTAPYDIEISFEISETYLQLGMLLTGEIEYDLKESHNNHLEPSSFYL
                     "
     gene            443..1351
                     /locus_tag="PLK68_00110"
     CDS             443..1351
                     /locus_tag="PLK68_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM88842.1"
                     /translation="MKKVLSTILVLSLFILTGCSAATKDTIRTVETVKGKIEIPNEPK
                     RVVIDYFVGDALALGVEPVGTTYVYKDSVFEEDLKDVPSINGDDAYGQYSMEAVTKLK
                     PDLIITYSEADYDNLSKIAPTVLVDYLNMSTAERITWMADVLNKKEEGKKLLADFNQE
                     VAGYKQQLQDAKISNKTITLMETYSKELFVYGNKQGRGGEVLYDLLELKAPEVVQKEI
                     INGEQYRSISLEAINEYAGDMIMIGGWQEDPMDLVGNNSVWNSTPAVKNQKVITYDSS
                     AYIYQDILSTKEQLKNITQSILALYE"
     gene            1344..2339
                     /locus_tag="PLK68_00115"
     CDS             1344..2339
                     /locus_tag="PLK68_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM88843.1"
                     /translation="MNNQQVRKWLIVGLCLLPIIIFFSLKFGAVTIGWEDFFKIILGK
                     KSNGNDYEVIINLRFPRILATGIVGSAFAMAGAAMQGLTKNHLADSGILGINAGASFM
                     LAICFALGSRSNFLLMMMYSFVGAGLGLFLTMTMGKGKYTNNSKRLLLAGVAVSLFFT
                     SLSQFIAIYFGIGQELTYWNVGGTANIGYQELILATLLFVSGLFLLLRQGKNITIISM
                     SEETAVGLGVNVPRCKILVCLAALLLAGMAVSIVGSIGFIGLIVPYLVRRLVKKEDYQ
                     IILPMSAIYGAVFLILIDLIARNLQPPYETPLGIIMALIGVPFFLFMERSINKKC"
     gene            2414..>2846
                     /locus_tag="PLK68_00120"
     CDS             2414..>2846
                     /locus_tag="PLK68_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRM88844.1"
                     /translation="MKIELSELFDPYTQLVIFQFRLPRLLCAILVGGGMALAGHILQT
                     VLQNDLADPGVIGINSGISFFVLLYILVSGTDIFLDYVWMPLAGMIGAVITIGLLMFL
                     IYEKERGIDPIRMTICGVALNLGFQGLILFFSAKLNRQKFSY"
     assembly_gap    2847..2856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2857..3290
                     /locus_tag="PLK68_00125"
     CDS             <2857..3290
                     /locus_tag="PLK68_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRM88845.1"
                     /translation="GTDWLKIALLITILVIIGVILWKYQDVLNIFSLGKESVITLGVD
                     LKKQTIILISLAGFLAAFTIAFGGSIPFVGLIAPHIARRLVGSNHRYSLWVSMLMGSL
                     MVIIGDSLSRSLMSTGEIPTGIILSLIGVPYFIYLLKKERA"
     gene            3290..4066
                     /locus_tag="PLK68_00130"
     CDS             3290..4066
                     /locus_tag="PLK68_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM88846.1"
                     /translation="MKGIIEARQLSTGYGKKIVLKDQNIVIPKNKITVILGPNGCGKS
                     TMLKTMARVLPLKDGKIILDGKDLKTMKTAEIAQKIGFLSQILNTPEGISVYELVSYG
                     RYPYKRISSTKEDQKIIEWALKETQTFEIKDMLVHDLSGGQKQRVWIAMVLAQQTNTI
                     LLDEPTTYLDIGHQLEVLELLSKLNKEEQQTIVMVLHDINHASRYSDWIIAMKEGKVF
                     KEGSPKDIITSEVIQELYGVKTQIIFDKEHNCPVCFSYDL"
     gene            4177..4806
                     /locus_tag="PLK68_00135"
     CDS             4177..4806
                     /locus_tag="PLK68_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88847.1"
                     /translation="MQLTKEKLSYLLFLYKQREMDYTVTRLAQEVGVSKSTFSRVLNT
                     FYQEGLTTEKGKGILSCQGCRMAREYLKDINKLSDWLKYTANFDDEEAYQEALSLVLT
                     LSSKARAKLVNNTSKERLFELIDNVKEISGDMLSANLDDGQYLFAFTVYKADKMEISM
                     ANDGFFHPGILDIKMGHGGLVLKPKEVERESMMGKMVLKGKLSSLKYQG"
     gene            5183..6697
                     /locus_tag="PLK68_00140"
     CDS             5183..6697
                     /locus_tag="PLK68_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HRM88848.1"
                     /translation="MKKLLKVVLVLMLAFGIQIATLSNVDAAQVSDLPDGKYTIPTKL
                     KNASNIANDSMAAGALAENGELSVEEGKWYLTAEFKTLNLMGLVGNAGNIQYYETDTK
                     SEKHAAEVISYREDDQGKQQVEKVKIPVAVNSEGVYIEMFVDAMGTTVDAYIQYSTAD
                     IVIPEAPEYTLADGTYTVTSDVLKANEDVQSMAAQAVKSATVTSKDGTLLVTLQMGAV
                     TVYGQTAYVDKMEVEQADGTSYKEVDITGRDSDGNVSEMQFTLAKNTKLTNVKFYYGG
                     STHGSEARLSLGLDNPTLVVPESTSKFAKDGTYTVDVALWNATQDKASMAAGAIDSQA
                     TVVVKNGVATMYITTKEMTMGTIKAWLEELYIGSSTDDYKSNPAVIVSKNADGKATMW
                     SFVLPNEEELFDVVVNPHVAMMGNSDIPARMKVDYSTLKFVSDSIEAPKVDGESNNNT
                     NDTPNTTTPTTNQTTGTSSSSVKTGDNANMELMGGLLVSSLVAAAYLTRKRLCK"
     gene            6688..7590
                     /locus_tag="PLK68_00145"
     CDS             6688..7590
                     /locus_tag="PLK68_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding Shp domain-containing protein"
                     /protein_id="HRM88849.1"
                     /translation="MQIKKVLLILVAIITMTINIKPAFALDDGAYLIGRSTSYVNPLT
                     GNTEDGGTNITLGDSMVSSIVEGNLLLEQTGGKYYITVGLGLASNVSNVRFKIMNSGG
                     SFNSVSATKTGSSSANGDTVNHYRIQINSLNDYISPIMYVAPMGRDVQFFIKLNQGSI
                     TPGTGVYTSTMVPATADNGNSVNNNSTTNNSTSNNTSTNSNQTSDTTTNEEQPVVETP
                     VTTVGEVSRESLFDGISGLSSHVIDTNGKVNEKKKLTVKNLLKQSEQKKDSSNNTLLI
                     IGIVAVIAVVAGGGAYYYVKKVKK"
     gene            7568..8455
                     /gene="isdE"
                     /locus_tag="PLK68_00150"
     CDS             7568..8455
                     /gene="isdE"
                     /locus_tag="PLK68_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HRM88850.1"
                     /translation="MLKKLKNSFLVLVLALSMIGCVDQSGKSDNNNLTIAATSVAVTE
                     ILEALDVPADQVIGIPTTESYTVPKKYKKATELGTAMSPDMEVLSTLKPSLVLSPNSL
                     EGDLASKYEKIAVSSSFLNLKSVAGMFKSIEELGSLLGKEKKANKLVDEFVNYMVEFR
                     AKYQNTASPRVLILMGLPGGSYLVATESSYVGDLVKLAGGTNVYGNGNGQDFVNVNAE
                     DMLQQNPDIILRTSHAMPEEVMKNFETEFAQNDIWSKFSAVQNGKVYNLDNNYFGMSA
                     NFNYQKGLENLEGILYGTN"
     gene            8442..9425
                     /locus_tag="PLK68_00155"
     CDS             8442..9425
                     /locus_tag="PLK68_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM88851.1"
                     /translation="MELIKKHPRIFIIFLILVLFICFFIAINLGSIQVSIVQLFKGLF
                     IEYDVDVASVYSIRFPRIIISMLVGGALALSGLLFQVVLKNPLADPGIIGIANGASLV
                     SVLVGLFLPQLSAIAPLLSFFGGLITFAIIYSLSWKAGFKTTRIILIGVAINYTISAL
                     VTLANSATASITSGATGTISLYTWQDVTTLLIYLVPVIIITLFMAKACNLLGLEDRTL
                     TSLGVNVNVYRFGLSLLAVLLCSISVAVVGVIGFIGLLVPHISRLLVGTEHKHLIPIS
                     ILIGAIVLLVADTVGRIIMAPYEISAAIIMAIIGGPLFIILLKRSIDVDGS"
     gene            9415..10176
                     /locus_tag="PLK68_00160"
     CDS             9415..10176
                     /locus_tag="PLK68_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM88852.1"
                     /translation="MEVKKIKFSYQDKPFIEELSVKFKKNKITSIIGPNGSGKSTLLM
                     LLSRIYKAKDGTITLNDKNVWDYKIKEFAKNVAVVHQKNQIYGDLDVKTIVGYGRLPY
                     LSYHQNLSEEDYQIIDWAIATTNLKEYENRLLANLSGGQQQRVWLAMALAQKTPILLL
                     DEPTTYLDVKYQIEILNLIKEINKKYQMTIIMVHHDINQAINYSDEIIAMKDGKILFQ
                     GVPEEVITSASLKSLYDYDLSVIDYNHQKIVLNYQ"
     assembly_gap    10458..10495
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10496..11139)
                     /locus_tag="PLK68_00165"
     CDS             complement(<10496..11139)
                     /locus_tag="PLK68_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM88853.1"
                     /translation="MKIIIVGGGQIGSYITSLLLKNDHEVRVIENRTKALENYKNAGL
                     PEECLVIGDGTDIEVLEKAGVRTCQALVCVTGLDEVNLTTAMVSKFEYDVPRIIARVN
                     NPKNAWLFNSGMGVDAKINQADIIGHMIADEMNYQSIMTLMKLSKGDFSIVRIRVDYQ
                     SRYAGKQISEISLPNNALLIAIYNDDELMIPHGDTLIEAGQDILAFGDEQAIEK"
     gene            complement(11152..11799)
                     /locus_tag="PLK68_00170"
     CDS             complement(11152..11799)
                     /locus_tag="PLK68_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM88854.1"
                     /translation="MKIIIVGCGKFGTRLANYLNNQNHDVTIIDNREEAFNNLGGNFT
                     GRTVLGMGYDKDVLESAGIKVADALISCSSSDAINAVVSNIAHNIYHVNNVIARMYDK
                     SKARIFRSMGIHTISITDLGVERILDYFDSNRLQVVKKIGEDSEIKIIKIKASLSLIG
                     KTVDEISLHGEFEVVAIERHDITMMPLKDTKIKDNDTIYFSVLEESLLKFKEILY"
     gene            complement(11814..>14397)
                     /locus_tag="PLK68_00175"
     CDS             complement(11814..>14397)
                     /locus_tag="PLK68_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRM88855.1"
                     /translation="GGLLAILSGTVELGISIFCVNLINGFFSFFQEFKAEKATSALQK
                     MMPSYARVVRDGKEVKIFAEDIVPGDIMILEEGDRICGDARILRCSDFQVDQSTLTGE
                     SNPIRKNYEALQEKVSYLEAENMIFTGTTVASGTCHCVVVATGMDSEFGKIANLTQNT
                     EKSLSPLQKELNVLTKQIAIIALSVGIVFMLIAVFVIKDPLLESFIFSLGMIVAFIPE
                     GLLPAVTLSLALSVQRMAKDNALVKKLSAVETLGCTNVICSDKTGTLTQNEMTVNHLW
                     TLDSQMDVSGEGYVPNGKIYVDEQEITAKKSNVLRLLLSGAVLCSNAKLVPPQNKSVN
                     PRYTVLGDPTEACLEVVAKKAEIDLDKLNSQYPRILELPFESRRKRMTTIHQLKDSFE
                     GNQRIAFVKGSPKEVMELCNRCFKGSKACPISEEDRINIMKANDMYAREGLRVLAVAY
                     RTIAHNDKKLPSSIREYTPELIEQDLTFLGLIAMQDPPRSEVKEAVELCHSAGIKIVM
                     ITGDYGLTAESIARKIGIIKSDTAQIVSGTELSKMNDQELKNVLEGEVVFARMAPDQK
                     YRIVCALQEMGNIVAVTGDGVNDSPALKKADIGVAMGITGTDVAKEAADMILTDDNFA
                     SIVRAIEEGRAVYNNIRKFLRYIFDSNTPEAAAPALYLLSGGLVPMPLTIMQILTIDI
                     GTDMIPALGLGAEHPEDNIMNQPPRRIDERLLNKKVILVGFIWYGLIITLFALGGYFL
                     VNYLNGWPHNPLAPEGTALYNEATTMMLVGVVFSQMGMVMNNRTEKESVFKRGIFSNH
                     YINLGLVVEFVILLAVVYIPFLNGIFNTAPIGLIEWLYALPIPFIVFGIEELRKKILR
                     NKDK"
BASE COUNT         4942 a         2072 c         2614 g         4721 t
ORIGIN      
        1 tttaaataaa aactagaagg ctctaaatga ttattatgac tctcttttaa atcatactca
       61 atttcaccag ttagaagcat tcctagctgt agatatgttt cacttatttc aaatgatatt
      121 tcaatatcat agggtgcagt aaaatcacca acaaaaagtt catacatatc tgcaatatgc
      181 acatgaataa tgaattcttc aggttgttga ggatttatat ataaggaaaa gttatcatac
      241 tgatagtatt ggaaagctag tttattaaag aaaatattat agtactctgt caaatttcta
      301 catatcataa ttatcaccag ttaaattata cataaaataa atgtaaaaat acataatatt
      361 agtgaaaata ttgtaaaaga aattaagcca tgctagaatc acaacataag ttagctaaaa
      421 ctaactaaga agggagatat aaatgaaaaa agtactatca actatattag tgttaagttt
      481 atttatttta actggttgtt cagctgccac taaagataca ataagaacag ttgaaacagt
      541 aaaaggaaaa atagagattc ctaatgagcc taaacgtgtt gtaatagatt attttgttgg
      601 agatgcactt gcactaggag tggagccggt tggaactaca tatgtataca aagattctgt
      661 atttgaagaa gatttgaaag atgtaccttc aattaatggt gatgatgcgt atgggcagta
      721 tagtatggaa gcagtgacaa aattaaaacc ggatttaatt attacatatt ctgaggctga
      781 ttatgataat ttatctaaga ttgcaccaac ggtactagtt gattatttaa atatgtcaac
      841 tgccgaaaga atcacttgga tggctgatgt tttaaataag aaagaggagg ggaaaaaatt
      901 gcttgctgat ttcaatcaag aagttgctgg atataagcag cagttacagg atgctaaaat
      961 atctaacaag acgataactt tgatggaaac atatagtaaa gaattatttg tatatggaaa
     1021 taaacaaggt cgtggtggag aagttttata cgatttatta gaattaaaag cacccgaagt
     1081 tgttcaaaaa gaaattatta atggtgaaca atatcgttct atttcattag aagcaatcaa
     1141 tgagtatgcc ggtgatatga taatgattgg tggctggcaa gaagatccta tggatttagt
     1201 tggaaataat agtgtatgga actcaactcc agctgtaaaa aaccaaaagg taattacata
     1261 tgattcaagt gcatatatct atcaagatat tttatcaaca aaagaacagt taaaaaacat
     1321 tactcaaagt attttagcac tgtatgaata accaacaagt aagaaaatgg ctgatagtag
     1381 gactatgtct attacctatc atcattttct tttcattaaa atttggtgcg gtgacaatag
     1441 gatgggaaga tttttttaaa atcattttag gtaaaaagag taatggaaat gattatgaag
     1501 ttattattaa tcttagattt cctcgaattt tagcgactgg aatcgtcggt agtgcatttg
     1561 cgatggctgg agcagcaatg caaggactga ctaaaaatca tttagctgat tcgggaattt
     1621 taggaatcaa tgcgggtgct tcttttatgt tagcaatttg cttcgctttg ggcagtcgca
     1681 gtaattttct tttaatgatg atgtattctt ttgtcggagc aggattaggt ttatttttaa
     1741 cgatgacaat gggaaaaggg aagtatacta ataattctaa acgattatta ttagctggtg
     1801 ttgcagtctc acttttcttt acttcgttaa gccagtttat agcaatttat tttggaattg
     1861 gtcaagaatt aacatattgg aatgtaggcg gaactgctaa tattggatat caagaactta
     1921 ttttagcgac attattattt gtttctggtc tatttttgct tttgaggcaa gggaaaaata
     1981 ttacgattat ttcaatgagt gaagaaactg ctgttggttt aggggtaaac gttcctcgtt
     2041 gtaaaatact agtatgttta gcggcactgc ttttagcggg aatggcagtt agtattgttg
     2101 ggtcaattgg atttattggt ttgattgttc cttatttggt aagacgatta gttaaaaagg
     2161 aggattatca aattattttg cctatgtctg cgatatatgg agcagtcttt ttaattctta
     2221 tcgatcttat tgctagaaat ctacaaccac cttatgaaac accactagga ataattatgg
     2281 cactgattgg tgttccattt ttccttttta tggaaaggag cataaataaa aaatgttaaa
     2341 gaaaaaaaga ataatcattt ttctgataat cattttatta gtatcaagct ttttgtcatt
     2401 cacgataggt cagatgaaaa ttgaattaag tgaattattt gatccatata cacaattagt
     2461 aatctttcaa tttcggttac cgcgtttgtt atgtgccatt ttagtaggtg gtggaatggc
     2521 tttggcagga catattttac aaacagtatt acaaaatgat ttagctgatc cgggtgtaat
     2581 tggaataaac tcaggaattt cattctttgt tttgttatat attttagtta gtggaacaga
     2641 tatattttta gattatgttt ggatgccttt agctggaatg attggtgctg taataacgat
     2701 tggtttacta atgtttttga tttatgaaaa agaacgtgga atagatccaa ttcggatgac
     2761 tatatgtggg gtagctctta atttaggctt ccaagggtta attctattct tctcagcaaa
     2821 gctgaatcgt caaaagtttt cgtatgnnnn nnnnnncagg aacagactgg ctaaaaatag
     2881 ctttattaat aacaatatta gtgattattg gagtaatatt gtggaaatat caagatgttc
     2941 taaatatttt ttcgttagga aaggaaagtg taataacctt gggggttgat ttaaaaaaac
     3001 aaacaattat tttaattagt ttagccggat ttttagcagc ttttaccatt gcttttggag
     3061 gttctattcc ttttgtaggt ttaatcgcac cgcatattgc aagaaggtta gttggtagca
     3121 atcatcgtta ttcattatgg gtcagtatgt taatgggaag ccttatggtt attatcggtg
     3181 atagtttatc aagaagtctg atgagtactg gtgaaattcc aacagggatt attttatcat
     3241 tgattggagt gccgtatttt atttatctat taaaaaagga gagagcatga tgaagggaat
     3301 tattgaagct agacagttaa gcaccggtta tggtaaaaag attgttttga aagatcaaaa
     3361 tattgtaatt cctaaaaata aaattaccgt tattttagga ccaaacggat gtgggaaatc
     3421 aaccatgtta aagacaatgg ctcgggtatt accattaaaa gatggtaaaa taattcttga
     3481 tggaaaagat ttgaaaacta tgaaaacagc tgaaatagct cagaaaatag gttttctctc
     3541 acaaattcta aatacccctg aaggaataag tgtttatgag ttagttagtt atggtcgtta
     3601 tccatataag aggatatcat cgactaaaga agatcaaaag attattgagt gggctttgaa
     3661 agaaacccaa acttttgaga taaaggatat gttggttcat gatttatctg ggggtcaaaa
     3721 acaacgagtg tggattgcga tggtacttgc tcagcagaca aatacaattt tactagatga
     3781 accaacaaca tacttagata ttggtcatca attagaagta ttagaattat tgagtaaatt
     3841 aaataaggaa gaacaacaga caattgtcat ggttttacat gatattaatc atgcttcacg
     3901 ttatagtgat tggatcattg ctatgaaaga aggcaaagtt tttaaagaag gaagtccaaa
     3961 agatataata acttcagaag tgattcagga actttatggt gttaagacgc aaattatttt
     4021 tgataaagaa cataattgcc cagtgtgttt ttcatatgat ctttaagtaa taagtagtta
     4081 aaaatagcta tgttcatcat gaatatagct attttatctt gttttaaagg aataaatacg
     4141 ataatatgtt agcataaact aactttggag gggattatgc agctaacaaa agaaaaacta
     4201 tcatatttat tatttttata taaacaaaga gaaatggatt acacagttac tcggttagcg
     4261 caagaggtcg gagtatcaaa atcaacgttt agccgagtat tgaatacctt ctatcaggag
     4321 gggttaacga ctgaaaaagg gaagggaatt ttatcatgcc agggatgtag aatggcaagg
     4381 gaatatttaa aagatattaa taaattatct gattggttaa aatatacagc taattttgat
     4441 gatgaagaag cgtatcaaga ggcactatca ttagttttaa cactatcatc taaagcacgt
     4501 gctaaacttg ttaacaacac tagtaaagaa agattatttg aattgattga taatgtaaaa
     4561 gaaattagtg gagatatgct tagtgctaat cttgatgatg gtcaatacct gtttgctttt
     4621 actgtttata aagctgataa gatggaaata tcaatggcta atgatggttt ctttcatcca
     4681 ggaatattgg atataaaaat gggacatgga ggattagttt taaagccaaa agaagttgaa
     4741 cgagaatcga tgatggggaa aatggttctt aaagggaagc tgagttcttt aaaataccaa
     4801 ggatgattat attattccta tttcaaaatt acgttttcat tattcaaagg aagaacggat
     4861 attgcaaacg agtgtaaaga taaaggttaa accaacggtt ggtaaaattc atatgcctga
     4921 gagtattgca attatgaacg ttattataaa atagtatttc atcgatgaaa catcaaaaaa
     4981 ttctgtattt ataaagaaaa aagatatttt ttgaaaagat tgtttcaatg ggggcaacaa
     5041 aattagtagt tgttttaaga ttagatgtat gtaaaataat ggatgtagtt agttttagct
     5101 aacattagca gagaatatat tctctatatt ttttaactaa atagttagtc ttttctaaca
     5161 aagaaaaaga gggaggaaga aaatgaaaaa acttttaaaa gtagtattag ttttaatgct
     5221 ggcatttgga attcagatag ccacattaag taatgtagat gctgcccagg taagcgattt
     5281 accagatggt aaatatacta ttccaacaaa attgaagaat gcaagtaata ttgcaaatga
     5341 ttcaatggca gctggagcat tagcagaaaa tggagaatta tcagtagagg aaggaaagtg
     5401 gtatttaact gctgaattta aaacattaaa tttaatgggc ttagttggta atgcaggaaa
     5461 tatccaatat tatgaaactg atacaaaatc agaaaaacat gctgctgaag ttattagtta
     5521 tcgcgaagat gatcaaggaa agcaacaagt agaaaaagtt aaaatacctg tagcggtaaa
     5581 cagtgagggt gtttatattg aaatgtttgt tgacgcgatg ggtacgacag ttgatgcgta
     5641 tattcaatac agtacagcgg atattgttat tccagaagca cctgaatata cattagctga
     5701 tggtacttat actgtgacta gcgatgtatt aaaagcaaac gaagatgtac aatcaatggc
     5761 tgcccaagcg gtcaagagcg ctacggttac ttcaaaagat ggaactttat tagtaacatt
     5821 acaaatgggt gcagttactg tgtatggtca aactgcatac gttgataaaa tggaagttga
     5881 gcaggctgat ggaacttctt ataaagaagt tgatatcaca ggacgtgata gtgatggaaa
     5941 tgtaagtgaa atgcaattta cattagcaaa aaatacaaaa ttaacaaatg taaaattcta
     6001 ctatggtgga agtactcatg gttctgaggc gagattatct ttaggattgg ataatccaac
     6061 actagtagta ccagaaagta caagcaaatt tgctaaagat ggtacatata cagtagatgt
     6121 tgctttatgg aatgcgactc aagataaggc ttcaatggca gcgggtgcaa ttgacagtca
     6181 agctacagta gtagttaaaa atggtgtagc aacaatgtat atcacaacta aagaaatgac
     6241 tatgggaaca attaaagcat ggttagagga attatacatt ggttcatcaa ctgatgatta
     6301 taaatcaaat ccagctgtta ttgtaagtaa aaatgctgat ggtaaagcaa ctatgtggtc
     6361 tttcgtttta ccaaatgagg aagaattatt tgatgttgtg gtaaatccac atgtagctat
     6421 gatggggaat agtgatattc cagcaagaat gaaagttgat tatagcacat taaaatttgt
     6481 atctgattca attgaagcac ctaaggtaga tggtgaatca aataataata caaacgatac
     6541 accaaataca acaactccaa cgactaacca aactacagga acatcaagta gttcagtgaa
     6601 aactggagat aatgctaata tggaattaat gggaggatta ttagtttcat cattagtggc
     6661 tgcggcatat ttaacaagaa aaagattatg caaataaaaa aagtattatt aatattggtt
     6721 gccatcataa cgatgacaat taatattaaa ccagcttttg ccctagacga tggagcgtac
     6781 ttaattggac gctccacgtc ttacgttaat cctctaactg gtaacaccga agatggtggt
     6841 actaatatta cattaggtga tagcatggtt tcaagtattg tagaaggcaa tttgttatta
     6901 gagcaaactg gtggtaaata ttatattact gttggtttgg gattagcttc aaatgtttca
     6961 aatgtaagat ttaaaatcat gaatagtggt ggttctttta atagtgttag tgctactaaa
     7021 acaggaagta gtagtgctaa tggtgacacg gtaaatcatt atcgaattca aattaattca
     7081 ttaaatgatt atattagtcc tatcatgtat gtcgctccaa tggggcgtga tgtgcaattt
     7141 tttattaaat taaatcaagg atctattact ccgggaacag gtgtatacac tagtaccatg
     7201 gtaccagcca ctgccgataa tggaaattcg gtaaataata attcaactac taataatagc
     7261 accagcaata atacaagtac gaattctaat caaactagtg atacgacaac aaatgaagaa
     7321 caaccagtgg tagaaacgcc ggtaactaca gtgggggaag tgtcacgtga aagtttattt
     7381 gatggtattt ctggcttatc tagccatgta attgatacaa atggcaaagt taatgaaaag
     7441 aagaaattga cagttaaaaa tttattaaaa caaagtgagc aaaaaaagga tagtagtaat
     7501 aatacgctgt tgattattgg aattgtagcg gttatagctg tcgtagcagg agggggagca
     7561 tattattatg ttaaaaaagt taaaaaatag ttttcttgtt cttgtccttg ctttgtcaat
     7621 gattggttgt gtagatcaaa gtggaaaaag tgataataat aatttgacga ttgcagcaac
     7681 ttcggtagct gttacggaaa ttttagaagc tttggatgtt ccagctgatc aggtaattgg
     7741 cattccgacg actgaaagtt atacagtacc aaagaaatat aaaaaagcga cagaactagg
     7801 aactgcaatg tcgccagata tggaagtatt gtcaacttta aaaccatcgc tagtattaag
     7861 ccctaattca ttggagggtg atttagcttc taaatatgaa aaaattgctg taagttctag
     7921 tttcttaaat cttaaaagtg tagcaggtat gtttaaaagt attgaggagt taggaagctt
     7981 attaggtaaa gagaaaaaag caaataaatt agtagatgaa tttgttaatt atatggttga
     8041 atttagagct aaataccaaa acacagcaag tccacgggtt ttaattttga tgggtttgcc
     8101 aggtggcagc taccttgtcg ctacagaatc tagctatgtt ggtgatttag taaaattagc
     8161 tggtggaaca aatgtttatg gtaatggtaa tggtcaggat tttgtcaatg tcaatgctga
     8221 agatatgtta caacaaaatc ctgatattat cttgagaact agtcatgcga tgccagagga
     8281 agtaatgaag aattttgaaa ccgaatttgc tcaaaatgat atttggagta aattcagtgc
     8341 agttcaaaat ggaaaagttt ataatttaga taataattat tttgggatga gtgctaattt
     8401 taattatcaa aaaggcttag aaaatttaga aggaatttta tatggaacta attaaaaaac
     8461 atccgcgaat atttataatc tttttaatat tagtattatt tatctgtttt tttatcgcaa
     8521 taaatcttgg aagtattcaa gtttcaattg ttcaattgtt taaagggtta tttattgaat
     8581 acgatgtcga tgtagcaagt gtatatagta ttcgctttcc aagaattatc atctccatgt
     8641 tagtaggtgg tgcattagca ttatctggac ttttatttca agttgtttta aagaatccgt
     8701 tagctgatcc gggaatcatt ggaattgcta atggggcgag tttagttagt gtactagttg
     8761 gtttattttt gcctcaacta tctgcgattg ctcctttact atcattcttt ggaggtttaa
     8821 taacatttgc aattatttat agtttatcgt ggaaagctgg atttaaaaca actcgaatca
     8881 ttttgattgg ggtggcaatc aactatacga tcagtgctct tgttaccctc gcaaatagtg
     8941 caactgcttc aatcacaagt ggtgctacgg gtactatttc cttgtatact tggcaagatg
     9001 ttacgacctt attaatttat ttggttcctg taataatcat tactttattt atggcgaagg
     9061 catgtaatct attagggtta gaagatcgaa cccttacttc gctaggagta aatgtgaatg
     9121 tatatcgctt tggtttatca ttattagcag ttttattatg cagtattagt gtagcagtag
     9181 ttggggtaat tggctttatt ggtctgctag tacctcatat ttcaagattg ttagtgggaa
     9241 ctgaacataa acatttgata ccaattagta tattgatagg agcaattgtt ttgttagttg
     9301 cggatacggt aggaagaatt attatggctc cctatgagat ctcggcagcc attattatgg
     9361 ctatcattgg tggaccttta tttattatct tattgaaacg gagtattgat gttgatggaa
     9421 gttaaaaaaa ttaaatttag ttatcaagat aaaccgttta ttgaagaact ttcggtaaaa
     9481 tttaaaaaga ataagattac atcgatcatt ggccctaatg gtagtggtaa atcgacttta
     9541 ttaatgcttt taagtcgtat ttataaagcc aaagatggaa caataacatt aaatgataaa
     9601 aatgtctggg actataagat caaggaattt gctaaaaacg ttgcagtagt tcaccaaaag
     9661 aatcagattt atggggattt agatgttaag acaattgttg gttatggacg tttaccatat
     9721 ttaagttatc atcaaaatct aagcgaggaa gattatcaaa ttatagattg ggccattgca
     9781 acaactaatc taaaagaata cgaaaatcgt ttgttagcga atttgtctgg tggtcagcag
     9841 cagcgtgttt ggttggcaat ggctttagct caaaaaacac ctattttatt actggatgag
     9901 ccaactacat atttagatgt taaatatcaa attgaaatac ttaatttaat aaaagaaata
     9961 aataaaaaat accaaatgac gattattatg gttcatcatg atattaatca agcaatcaat
    10021 tatagtgatg aaatcattgc aatgaaagat gggaaaatct tatttcaagg agttcctgag
    10081 gaagtaataa cttcagcatc attaaagtct ttatatgatt atgatttatc agtgattgat
    10141 tataatcatc aaaaaatagt attaaattat caatagctaa cataatttac cgttgttaga
    10201 agttaatctt agatatttat aatttaatct agcgatattt aattactata ctaatgaatg
    10261 tagtcaggat tgtttaaata gtttttcaat cccttctcca aatgactact cttcatgata
    10321 ttctttccgg atatcaataa atttagatag agtcccacta tctgtactca aaatgaaaaa
    10381 cagccattaa ataatctttt atggaaactt cctaaagtaa atagagtttt ctatatagaa
    10441 aagttaattg gctgtttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaattt
    10501 ttcaattgcc tgctcatcgc caaaagcgag aatatcttgt ccggcttcaa ttagcgtatc
    10561 tccgtgagga atcattaatt catcatcatt gtaaatcgca attaagagag cattgtttgg
    10621 taaagaaatt tcagaaattt gtttgccagc atatcgtgat tgataatcaa ctcgaattcg
    10681 cacgattgaa aaatcacctt tagataattt cattaaggtc ataattgatt gataattcat
    10741 ttcatcagca atcatatgac caataatgtc agcttgattg attttagcat caactcccat
    10801 tccagaatta aataaccagg cgtttttagg gttattaaca cgagcaatta ttcgtggaac
    10861 gtcatattca aatttagaaa ccatggcagt agttaaattg acttcatcta atccagttac
    10921 acagactaag gcctgacaag ttctaacgcc tgctttttca agaacctcaa tatctgttcc
    10981 atcgccgatt actaaacatt cttctggtag tccggcattt ttatagtttt caagtgcttt
    11041 agttctattt tcaataacac ggacctcatg gtcattttta agtagtaaac ttgtgatata
    11101 agaaccaatt tgacctccac ctactataat tattttcatt tttttcacct cttaatacaa
    11161 aatttcttta aattttaata atgattcctc tagtacagaa aaataaatcg tatcattatc
    11221 ttttatttta gtatctttta aaggcatcat tgtaatatca tgtcgttcaa ttgcgacaac
    11281 ttcaaactca ccatgaagag aaatttcatc gactgttttt cctattaaag ataaggatgc
    11341 tttaatttta ataattttta tttcactgtc ttcaccaatt tttttaacta cttgaagccg
    11401 attactgtca aaataatcta agatcctttc aacacccaaa tcagtaattg aaattgtatg
    11461 aattcccatt gaacgaaaaa tccgtgcctt tgatttatca tacattctgg cgattacatt
    11521 attaacatgg taaatattat gagcaatatt actaacaaca gcattaatag catcactgct
    11581 tgaacaactg atcaaagcat cagcaacttt gattccggca ctttctagaa catctttatc
    11641 gtaccccatt cccaatactg tccgtccggt aaaattacca ccaagattat taaaagcctc
    11701 ttcacgattg tcaattatag tgacatcatg attttgatta tttaaataat ttgctaatcg
    11761 ggtaccaaat tttccacacc caacgattat aattttcata ggtttccatc ctcctatttg
    11821 tctttatttc ttaatatttt ctttcttaat tcctcaattc caaaaacaat aaaaggaatt
    11881 ggcaaagcat atagccattc tattaaccca attggcgcag tattgaagat accatttaaa
    11941 aaaggaatat agactacggc taataaaata acaaactcta caactaatcc taaattaata
    12001 taatgattgg agaagattcc gcgtttaaat actgattctt tttcagttcg gttgttcatg
    12061 accattccca tctgtgaaaa aacaacccca actagcatca tcgtcgttgc ttcgttatag
    12121 agcgcagttc cttctggagc caatggatta tgaggccaac cattcaaata gtttacaagg
    12181 aaatatcctc ctaaggcaaa tagcgttata atcaagccat accaaataaa acctactaaa
    12241 attacttttt tattaagtaa tctttcatcg attcgacgag gtggctgatt cataatatta
    12301 tcttcaggat gttctgcccc taaaccaagc gcaggaatca tatcagttcc aatatcaata
    12361 gttaaaattt gcatgattgt aaggggcatt gggactaaac cgccactaag caaatataga
    12421 gcaggggcag cagcttctgg ggtgttgcta tcaaaaatat aacgtaagaa tttacgaata
    12481 ttattataaa cagcacgacc ttcttcgatt gcacgaacaa ttgaggcaaa attatcatct
    12541 gttaaaatca tgtcagcagc ctctttagca acatcggtac cagttattcc catagcaaca
    12601 ccaatatccg ctttttttag agccggagag tcattaacac catcaccagt tacagctaca
    12661 atatttccca tttcttgtaa agcacaaaca atacgatatt tttgatctgg tgccattcga
    12721 gcaaagacca cttcgccttc taaaacgttt ttaagttctt gatcattcat tttgcttaat
    12781 tctgttccag aaactatttg agcggtatcg cttttaataa tcccaatttt gcgagcaata
    12841 ctttctgctg ttaaaccata atctccggta atcataacga ttttaattcc ggcactatgg
    12901 cataattcca cagcttcttt gacttcactt cgtggagggt cctgcattgc aatcaagcct
    12961 aaaaaagtta gatcttgttc aattaattca ggtgtatatt cgcgaattga actaggaagt
    13021 tttttatcat tatgagcaat agtacgatag gctactgcta aaactcgcaa cccttcacga
    13081 gcatacatat cgtttgcttt cataatattg atgcggtctt cttcactgat tggacaggct
    13141 ttactgcctt taaaacaacg attacataat tccataactt cttttggaga cccttttaca
    13201 aatgcaattc tctgattgcc ttcaaaagag tcttttaatt gatggatcgt tgtcattcgc
    13261 ttccgtcgag attcaaaagg taattctaaa attcgtggat attgactatt caatttatca
    13321 agatcaattt cagctttttt agctacaact tctaaacatg cttcagtagg gtcacctaaa
    13381 actgtataac gtggattaac actcttattt tgtggtggta ctaacttagc gtttgaacaa
    13441 agtactgcac cacttaataa tagtcttaaa acattacttt tctttgcggt aatttcttgt
    13501 tcatcaacat agatttttcc atttggaaca tatccttcgc cgctgacatc catttgacta
    13561 tcaagagtcc aaagatgatt aaccgtcatt tcattttgag ttaaagtacc agttttatca
    13621 gaacagataa catttgtaca tcctaaagtt tctacggctg atagtttctt tactaaagcg
    13681 ttatctttag ccattcgttg aacagataaa gcaagtgata acgtaacagc gggtaaaaga
    13741 ccttcaggaa taaaagcaac aatcattcct aaagaaaaaa taaatgactc caataaggga
    13801 tccttaatta caaatacagc aatcagcata aacacgattc caacacttaa tgcaataatt
    13861 gcaatttgtt tagttagaac attaagttct ttttgtaatg gagataagct tttttcagta
    13921 ttttgagtta aattagcaat tttcccaaat tcactatcca ttccagttgc aacaacaaca
    13981 cagtggcaag taccagaagc aacagtagta ccggtaaaga tcatattttc agcttctaag
    14041 tatgaaactt tttcttgcag tgcctcataa ttctttctaa ttggattact ttcacctgtc
    14101 aaagttgatt gatctacttg gaaatcacta caacgtaaga ttctagcatc cccacaaatt
    14161 cggtcacctt cttctaaaat cattatatca ccaggaacaa tgtcctcagc aaatattttt
    14221 acttctttac catcacgtac tactcgggca taagatggca tcatcttttg caacgcacta
    14281 gttgcttttt ctgctttaaa ttcttggaag aaagaaaaga agccattaat tagattgaca
    14341 cagaaaatcg atattcctaa ttcgactgtt ccagaaagga tagctagtaa tcctcca
//
LOCUS       DAOMTA010000003        14298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_13_length_14298_cov_2.641297, whole genome shotgun sequence.
ACCESSION   DAOMTA010000003 DAOMTA010000000
VERSION     DAOMTA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_14298_cov_2.641297"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1063..1593
                     /gene="rnmV"
                     /locus_tag="PLK68_00180"
     CDS             1063..1593
                     /gene="rnmV"
                     /locus_tag="PLK68_00180"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HRM88856.1"
                     /translation="MKKIKEIVVVEGKTDTALLKELFEVDTIETHGLALDQQTLELIQ
                     EASKSRGIIVLTDPDFPGKKIRDQIQAVVPNCQHAFVAKKDARGKKKLGIAEANKEAV
                     IEALENVVSFDVNRESITWSEFVALDIIGNKQRRLMVYEAFNLGYGNVKTLFKRLNMV
                     GITKEQVLGRLNDGSR"
     gene            1580..2119
                     /locus_tag="PLK68_00185"
     CDS             1580..2119
                     /locus_tag="PLK68_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88857.1"
                     /translation="MDLGKHNGYTFKVSNEFKGEYLVPIIDNLKVKYRDFYDDYDEVY
                     HCNQLVHILKDDPNVNVDYHVMYKYDQCVGLAMVTTGNIDSSKYFDKPVFEQGANAIV
                     LNYFHISPKARGNGNYWLRNVIIPHYQDKEDLYLKSSHSKAFSLYQRLGEEIGSYQTL
                     SDNGEFKRTGKIFKIKVNQ"
     gene            2119..>2309
                     /locus_tag="PLK68_00190"
     CDS             2119..>2309
                     /locus_tag="PLK68_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase A"
                     /protein_id="HRM88858.1"
                     /translation="MKEIATPSTTKYILEKYHLNALKKYGQNFLIDVNIINKSSLGIR
                     LKIQLGVWIEFLTNMSVVI"
     assembly_gap    2310..2319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2320..3012
                     /gene="rsmA"
                     /locus_tag="PLK68_00195"
     CDS             <2320..3012
                     /gene="rsmA"
                     /locus_tag="PLK68_00195"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRM88859.1"
                     /translation="IVTSAKIDQTTAVIEVGPGIGALTQVLGRYSGKVTSFEIDERFM
                     PVYQEFLNQDNIEIIFGDFMEQDIKPIVDQLKQRYQKVCLVANLPYYITTAIIEKVVL
                     GNFGIDEMIVMVQKEVALKMTGIYKNPLLLMIKDMGTIEYLFTVNKNVFMPAPHVDSA
                     ILKIELKKAPDLKLYEVLNICFKQRRKTILNNLKQSYEEAEEILLQTGIENRKRSEEL
                     ELADFKNITKMI"
     gene            3039..3782
                     /locus_tag="PLK68_00200"
     CDS             3039..3782
                     /locus_tag="PLK68_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HRM88860.1"
                     /translation="MKIGDIVCRKKYGKDICFKITDIQDDVYYLTGIEYRLVADSDES
                     DLELSDFSSEKSDIIVESRPCLKGSVLHIDGDKHYLDMCLKKYEEFNINVHGYFMKEN
                     EIKDQIIPLLEKHRPNLLVITGHDAMKKNGNRKDINSYLHTKDFVEAIRRARLYEDDK
                     DSLIIFAGACQSYYELLLASGANFASSPSRKNIHALDPVFVVSQIANASIKNYVDLEK
                     IVAKTSNKHLGVGGIDTRGVARKIYPTSR"
     gene            3787..4644
                     /gene="ispE"
                     /locus_tag="PLK68_00205"
     CDS             3787..4644
                     /gene="ispE"
                     /locus_tag="PLK68_00205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRM88861.1"
                     /translation="MKVKAYAKINLALDVIGKREDGYHELEMIMAPITLHDLIYINTI
                     ASGIEIDSNSKIMPTDERNIMYKVVALMKERYNIKKGVKIFVYKHIPTQAGLAGGSAD
                     GAAVIKAMNKLFYLNLSNEEMAALGKEVGADIPFCIYQKIALVSGVGEKLKFIKNSFE
                     CKVLLVKPKRGVSTKKSFTSLDLSKASHQDCRLMATGIEDGNYQIVIDNLQNTLEEPS
                     IKMVPEIAKIKEEMMKIGFDGALMSGSGSCVFGLTRNNIILEKGMKYFKGKYYFVRKT
                     EILNDEEID"
     gene            4744..6123
                     /gene="glmU"
                     /locus_tag="PLK68_00210"
     CDS             4744..6123
                     /gene="glmU"
                     /locus_tag="PLK68_00210"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRM88862.1"
                     /translation="MKTYAVVMAAGKGTRMKSDKPKVVHEVLYKPMINHIVDELKQVG
                     VDEIYVIVGHKAEEVEKLLDGVNIIYQKEQLGTGHALMQCKDALAGKAGTTVVLNGDA
                     PLITSETLKDLIAYHNDNQLKGTIMTCDCDLDKKFGRVIRNNDQVTGIVEFKDCTPEQ
                     VQISEMNCGEYCFDNIAVFEALEKVTNDNAQNEYYITDVIEIMNNDKLKVGGYKIADL
                     AEVGGINDRVELAEATKSLQLKINKQHLLNGVNIIDINNTYIGVDVTIGADTTIEPGC
                     IIKGKSSIGSNCHIGPYCEFDNVEIKDNVEIKFSVISDSIIENGVDIGPFARLRTNCH
                     ILEDAHMGNFVEMKKAVFGKGSKASHLTYVGDAAVGSNVNMGCGTITSNYDGKNKFQT
                     IIGDNAFIGCNSNLVAPVTVGANAYVAAGSTITDQVEDSAFAIARARQVNKDGYAKVL
                     EEKRNQKGK"
     gene            6133..7089
                     /locus_tag="PLK68_00215"
     CDS             6133..7089
                     /locus_tag="PLK68_00215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRM88863.1"
                     /translation="MKNKITVFALSASGELANDIAEKLGTKVGKSKVHHFADGEILVE
                     IDESVRGKDVFIVQSTSNPVTENLMEILVLADALKRASAKEITAIIPYFGYARQDRKA
                     KPRQPITSKLVADLLTVAGVTRVVTVDLHAAQIQGFFDIPVDEMQALPLISNYFKKKD
                     MEDICVVSPDHGGATRARKLAVALDAPVAIIDKRRPKPNVAEIMGVLGDVSGKNCIMV
                     DDMIDTGGTIVAGIEMLKEKGAKSVHVACTHPVFSGPAVERLQNSSADEVVVTDTIKL
                     PEDKMFPKLKTVSVAGLLAKTIENIENCLPVSDVFEMFDFDK"
     gene            complement(7194..8342)
                     /locus_tag="PLK68_00220"
     CDS             complement(7194..8342)
                     /locus_tag="PLK68_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM88864.1"
                     /translation="MQYRGSYLSYLLMYLFYFLSLALLSGLISVYLLDRGYSASQVSL
                     VVASSYIVSVILQPLVGYLNDHFNLKWVNSVVLIIAAILGIALIFAKHLFSITLLYSL
                     TLGLFNSTKPVIERMATLSKYQYGKIRIWGTIGYAIGSQIGGLIYQYISPESMYFFFS
                     ISLGICLLGIIGTRNEHKNSDIHGETTTQHLGLWNKNFIVYLIIVCLFYAITNLNTTY
                     LPAMFQHQGISVDKVSTIILLITISELPIIYYSHRFMNNISNRHMLIIVFILLIIQFG
                     TYCFIPYNIIHVIISIGTKAVSTVIFIMLNMKIVVSIVDSDLQMSALAWVSTFNSFSS
                     IVGQGLGGKILDTYSYTDFYLILFLIAVIGLGISYFYHLPSGRKYHLF"
     gene            8509..9396
                     /locus_tag="PLK68_00225"
     CDS             8509..9396
                     /locus_tag="PLK68_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM88865.1"
                     /translation="MLLKEQMKAYPFSHNERVIIDYILDKQINIKDYSVKMIADATFT
                     SPSTLIRISKKLGFQGWVEFKDAYLEEANYLNSHFCNIDSNLPFSNQDSIMTIASKIV
                     NLHIESAKDTLSLFQHDSLQKAVRILHQSKEIRVFAMSNLNFAGEEFVFKLNRIHKKA
                     YIHPIQDNLFHDAAMSSPDECAICISYSGESSNIVKTAQILKENKCPIIAITSIGNNS
                     LSDLATVTLRVTTREKSFSKIAGFSSLESISIVLNTLYACLFSLNYHDNLTYKLDIAK
                     KIENAPIINNEIIAEKDDE"
     gene            9659..>10123
                     /locus_tag="PLK68_00230"
     CDS             9659..>10123
                     /locus_tag="PLK68_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM88866.1"
                     /translation="MDKKILRFALLSASLLVGSAAAINANIPAMAQHFDQVPLSMVEM
                     LTTIPSLFLMISVLTSSLIAKRVGYKQTITIGLGIVMIAGIVPLLIDNFMIILISRAM
                     LGFGVGLFNSLLVSMINYFYDAKERSSMYGLQSAFEGAGGIAITFIAGQLLKI"
     assembly_gap    10124..10151
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10152..10880
                     /locus_tag="PLK68_00235"
     CDS             <10152..10880
                     /locus_tag="PLK68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM88867.1"
                     /translation="QAPFIAYMIAIPVFFIYFKFVPQVKTADVIKANGGDQIKKESHK
                     SAGFLPVVYYVGLIFMAAMLYMIMGIKIASLMTGEGYGTASDASLVIILLSLGGISAG
                     LLFGKILKVFNQLTTSIGLIILALAMVILGLSQNLVITFVGGYLTGFGFKIFMPSLID
                     KINNSNIPNTTLATSLLLVGFNLGVFISPYGSIVIQSLMRTEALPALFIANAIGFISL
                     STITLIITLIKNKKTVNIVQKVLE"
     gene            10927..11811
                     /locus_tag="PLK68_00240"
     CDS             10927..11811
                     /locus_tag="PLK68_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM88868.1"
                     /translation="MDLRVLEYFLAVVESGNVSKAAQKLHLTQPTLTRQLQQLEEIYG
                     TRLFIRGSRQITLTNSGIILKRRAKEMLDLQDKTAAEIRKSEYDISGEITIGCGESSG
                     NKFLPVVLKEFSKRYPKIKYNIYTAGSNQNKEKINEGLIDVAIVLEPIDKDYYQYLEL
                     PYLDKWCLVMKKDDLLKEYETIENHVVSNLVLGVANRLEVKETFKNWLGKEINAQTIL
                     NYDINSNLALLVESGLCYGISIDGVFKSNAYPNLTYRLLEPEIVSQSCLIWKKGVVTN
                     QALEKFIVCAKELISSNQ"
     gene            12026..>14298
                     /locus_tag="PLK68_00245"
     CDS             12026..>14298
                     /locus_tag="PLK68_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88869.1"
                     /translation="MDSKMGKNLNKALISVLSLGMIVSMCSITVNAVETDNVANWVTM
                     YGDNGNNSITPDTVNDAERLINGDIIATGTFDGNKVTGIEQAKGKSDGAVMLYDKNGQ
                     IKWETLIGGSAADSFNAVTEANDGSYVAVGVSQSNDGNLANLNKGGKDGIIAKLDSNG
                     TLIKVVTVGGSDADELKAIQPAGDGSYLAAGYSSSSDGDLEGLAHGGKDAFVVKFNEN
                     GTKEWVKAFGGTGDDVFNHIIRAHNKKVSTDKTEISSSDIDNGYVLTGTTNSNSGIVG
                     NEEENNVAFTLKIDSNGQVEWTTSLANNSKTTGDYLLAISDGFLAVGTFDTNDQDFTG
                     IKTYGKEDIYIAHYSKDGTCLNISSYGGDGKDNPEGIIPGANDDYIVYGNTNSNSGLF
                     EGKLKGKYEGFLASLAGTTLEQYAEEKYLVPVEAWKANEDSLSMMAPLLYKDAYVEKI
                     GFKYTVTIYFTNATIMGTQVSASTLGEVSYEKNGEMIAVLKDEYDEVTQVKTVTLEVD
                     DLDTPVKLHIDKAMGDIRLSFSSKDMVETTNPPYFAPVEVEQPDFKTSWKVNIGGSDY
                     DYTDDMTVLKNGNIVAVGQSYSNDGDFQNLLKGGSIAYINQYDPKGELLKTLTLGGTE
                     YDSIAYAAKVCGLDDGGFVVSGGYQEGVYVAPTGDFAQLDTAGTVHGQMDTFIAKYDS
                     NSKLIWMSNFSGSANDQVKQIKATDDGGIAVLIETNSNDGDMENQNRGLYDLVIVKYD
                     AHGKKVWQRVLGGKNIET"
BASE COUNT         5143 a         1716 c         2765 g         4636 t
ORIGIN      
        1 gtacattgat tgaattaaca ttgaaatcat cgggggaatt gccattttca ttaataatgg
       61 gaagatgggc ttgtgagcca tcatatcata ttcttgattc ataagttctc cttccttcgc
      121 aaaaaaaagc tataacctcc cgtttataac tcagaaacgt ggaaaagcta tagcttatta
      181 tttaagcggt ttattaagtt tgcacatcaa gcataacgta attttatcat gatgtcaatt
      241 agataatttt atctttttaa gacttgatca aagcgttgat acaatcaaga tagaaatggg
      301 taatctgagt cgttgctctg ttcatggtac tgtaaaacca tttgaccaat ttctttacca
      361 aaaatatcat tgatcatttt ataggattct agagagtatt tatgattttt ttcagcagtt
      421 gttaaaggac ctgttttaag cagaatactt gtaggaatat taatttttca atattgtgaa
      481 gtatggaaga aattatttaa tctggtatca gacatattta actcatgagc aactttcatt
      541 gctaattttt gaactcaaat gcaatgttct ttggtatgtt aacctttagc ttgaagtatt
      601 gtcatgattg tatttaacat agtgattagc tttttatgat aatataactc attattgctg
      661 tacaattaaa aacagtagct ttaataattt gaaaaggata ttgtattgtg attggggaat
      721 tattaaaata tcactaggga tcgatagttt gttttttcaa ccagataata ttacgtaagt
      781 ttctgtggcg tctcgagtat ctaaagcacc aaagacacta tgcaggattc ggtaacgaac
      841 tgccttaatg ctatttgcac aaaaagtaat ctagaaccat tttctttaac tattagttaa
      901 tcctttcatt gaattgtcct tttgattatt taattaattg tataaaaaga atattatggt
      961 gttgatttat attggttaga taaaatcgtt attatgggta gccccaagat tatacctttt
     1021 attttaagtg aaatatcgtt ataattagag ttaggtgaaa ttatgaaaaa aattaaagaa
     1081 atagtcgttg ttgaaggtaa aacagacaca gccttattaa aagaattatt tgaagtagat
     1141 acgattgaga cacacggatt agcattggat caacaaacat tagaattaat tcaagaagca
     1201 agtaaaagtc gaggaattat tgttttgaca gaccctgatt ttccagggaa aaaaattcgt
     1261 gatcaaattc aagcagttgt ccctaactgt caacatgctt ttgttgctaa aaaggatgct
     1321 agaggtaaga agaaattagg tattgctgaa gctaataaag aagctgtgat agaagcatta
     1381 gaaaatgtag tttcttttga tgttaatcga gaaagcatta cttggagtga atttgttgct
     1441 ttagatatta ttggtaataa gcaaagaaga ttgatggtgt atgaggcatt taatttgggt
     1501 tatgggaatg tcaaaacact ttttaaacga ttaaatatgg ttggaatcac taaagaacag
     1561 gtgttaggga ggttaaatga tggatctagg taaacataat ggttatacat ttaaagtaag
     1621 taatgaattt aaaggggaat atctagtccc gattattgat aatttaaaag tgaagtatcg
     1681 tgatttttat gatgactatg atgaagtata tcattgtaat cagctagtac atattttaaa
     1741 agatgaccct aatgttaatg ttgattatca tgttatgtat aagtatgatc aatgtgttgg
     1801 tttagcgatg gtaacaacgg ggaatattga ttcttcaaaa tattttgata aaccagtctt
     1861 tgaacaaggg gctaatgcaa ttgttcttaa ttatttccat atttcaccaa aggcacgagg
     1921 aaatgggaat tattggttaa gaaatgtaat tattcctcat tatcaagata aagaagattt
     1981 gtatttaaag tcatcacatt ctaaagcatt tagtctttat caacgtttgg gtgaggagat
     2041 tgggtcgtat caaacactaa gtgataatgg tgaatttaaa agaacaggaa agatttttaa
     2101 aatcaaggtg aatcagtaat gaaggaaatc gcaactccct cgacaacaaa atatatttta
     2161 gaaaaatatc atttgaatgc gttaaagaaa tatggtcaaa attttttaat tgatgtaaat
     2221 ataataaata agagttcgtt gggtataagg ctcaaaatac aacttggagt atggattgaa
     2281 ttcctaacca acatgagcgt tgttatttgn nnnnnnnnna tcgtgactag tgcgaagatt
     2341 gatcaaacaa cggcggttat tgaagtagga ccgggaattg gcgcattaac acaagtatta
     2401 gggcgttata gtggaaaagt tactagtttt gaaattgatg agcgttttat gcccgtgtat
     2461 caggagtttt taaatcaaga taatattgaa attatctttg gtgattttat ggaacaggat
     2521 attaaaccga tcgttgatca gttaaagcaa agatatcaaa aagtatgttt agttgctaat
     2581 cttccatatt atattacaac ggcaataatt gaaaaagttg tgttaggaaa ttttggaatc
     2641 gatgaaatga ttgttatggt tcaaaaagaa gtagctttaa aaatgacggg tatttataaa
     2701 aatccattat tattgatgat taaagatatg gggacaattg aatacttatt tactgttaat
     2761 aaaaatgtat ttatgcctgc tccgcatgtt gattcagcaa ttttaaaaat tgagttaaaa
     2821 aaagcaccgg atttgaaatt atatgaagta ttaaatattt gttttaaaca gcgacgaaaa
     2881 acgattttaa ataatttaaa gcaaagttat gaggaagctg aagaaatttt attgcagacg
     2941 ggaattgaaa atcgaaaaag aagtgaagaa ttagaattag cggattttaa aaatatcacc
     3001 aaaatgattt aattacatat aatatcatgg tgatataaat gaaaattggt gatattgtct
     3061 gtcgaaaaaa atatggcaaa gatatctgtt ttaaaattac cgatattcaa gatgatgttt
     3121 attatttgac tggaattgaa tatcggttag tagctgatag tgatgagagt gatttggaat
     3181 taagtgattt tagtagtgag aaaagtgata taatagtaga aagtcgacca tgtttaaaag
     3241 gtagtgtgct gcatattgat ggagataaac attatcttga catgtgtttg aaaaaatatg
     3301 aagaatttaa tatcaatgtt catggctatt ttatgaaaga aaatgaaatt aaagatcaaa
     3361 ttattccttt attagaaaaa catcgcccta atttattggt gataaccggt catgatgcaa
     3421 tgaagaaaaa tggtaatcgc aaagatatta atagttattt gcatactaaa gattttgtag
     3481 aagcaattcg tcgagcacgt ttgtatgagg atgacaaaga tagtttgatt atttttgctg
     3541 gagcgtgtca atcgtactat gagcttttat tagctagtgg agctaatttt gcttctagtc
     3601 caagcagaaa aaatattcat gcgcttgatc cggtttttgt agtaagtcag attgctaatg
     3661 cgagtatcaa aaattatgtt gacttagaga aaattgtggc taaaacttca aataagcatt
     3721 taggcgttgg agggatcgac acaagaggag tagcacgaaa aatatatccg acaagtaggt
     3781 gaagtaatga aagtaaaagc atacgcaaaa ataaatttag cacttgatgt aatcggaaaa
     3841 agagaagatg gatatcatga attagaaatg atcatggctc caattacttt acatgattta
     3901 atctatatta atactattgc tagcggaatt gaaattgatt caaatagcaa aatcatgccc
     3961 acggatgaac gaaatattat gtataaggtt gttgctttaa tgaaagaacg ttataacatc
     4021 aaaaaagggg ttaagatctt tgtttataag catattccta ctcaagctgg tttagcggga
     4081 ggaagtgctg atggtgcggc ggtaattaaa gcaatgaata aattatttta tttaaatctg
     4141 agcaatgagg aaatggctgc attaggaaaa gaagttgggg cagatattcc tttttgtatt
     4201 tatcaaaaaa ttgctttagt tagtggtgtt ggtgaaaaat taaaatttat aaagaattct
     4261 tttgaatgta aagtattatt agttaaacct aaacgagggg tatcaacgaa aaaatcattc
     4321 actagtttag atttaagtaa agctagccat caagactgtc gattaatggc gacaggaatt
     4381 gaagatggta attatcaaat agtcatcgat aatctacaaa atactttaga agagccgtca
     4441 atcaagatgg taccagagat tgctaagatt aaagaggaaa tgatgaaaat tggatttgat
     4501 ggagctttaa tgtctggaag tggatcgtgt gtttttggct tgacacgtaa taatattatt
     4561 ttagaaaagg gaatgaagta ttttaaggga aaatattatt ttgtaaggaa aacagaaata
     4621 ttgaatgatg aggaaattga ttaaatcaat ttcttttcaa ttttacgaca tattttatct
     4681 ttttttcctt taacaaatgt aaattttttg ttattatgtt gttgttaatg aagaggtgtg
     4741 aaaatgaaaa cttatgcggt agttatggcg gctggtaaag gtaccagaat gaagtcagat
     4801 aaaccaaagg ttgtccatga ggttttatac aagccgatga tcaatcatat tgttgatgaa
     4861 ttgaaacaag ttggtgttga tgaaatctac gtaattgttg gtcacaaagc agaagaagtt
     4921 gaaaaactgt tagacggggt aaatattatt tatcagaaag aacagttagg aactggacat
     4981 gcattaatgc aatgtaaaga tgctttggca ggtaaagcag gaacaactgt ggtgttaaat
     5041 ggtgatgctc cactaattac tagtgaaaca ttaaaagatt taattgctta tcataacgat
     5101 aaccaattaa aaggaacgat catgacatgt gattgtgatt tagataaaaa atttggtcgt
     5161 gtaattagaa ataatgatca agtaacgggg attgtcgaat ttaaagactg tactccagag
     5221 caagttcaaa ttagtgagat gaactgcgga gaatattgtt ttgataacat tgcagtattt
     5281 gaagcgttag aaaaagttac taatgataat gctcaaaatg aatattatat cactgatgta
     5341 atagaaatca tgaataatga taaacttaaa gtcggtggat ataaaattgc tgatttagct
     5401 gaagttgggg gaatcaacga tcgggttgaa cttgcggaag caactaaatc attacaatta
     5461 aaaataaata aacaacattt attaaatggt gttaacatta ttgatattaa taatacttat
     5521 attggagttg atgtaacgat tggtgctgat acgacaatcg aaccaggatg tattattaaa
     5581 ggtaagtcaa gtattggcag caattgccat attgggcctt attgcgaatt tgataatgtt
     5641 gaaattaagg ataatgttga aattaaattc tcagttatct ctgattcgat tatcgaaaat
     5701 ggtgtagata ttggaccatt tgctcgtcta agaactaact gccatatttt agaagatgct
     5761 catatgggta acttcgttga aatgaaaaaa gcagtctttg gaaaaggaag taaagcatca
     5821 catttaactt atgttggtga tgcagctgtc ggaagtaatg taaacatggg atgtggaaca
     5881 attacttcaa attatgacgg taaaaataaa ttccaaacta tcattggtga caatgccttc
     5941 attggatgta atagtaattt agttgctcca gtaacggtcg gtgctaatgc ctatgttgca
     6001 gcgggctcaa ctattacaga tcaagttgaa gattccgctt ttgcaattgc tcgggctcgc
     6061 caagttaata aagatggata tgccaaagta ttagaagaaa aaagaaatca aaaaggaaaa
     6121 taggagaaga aaatgaaaaa taaaataact gtctttgcat tatcagctag tggtgaatta
     6181 gctaacgata tcgcagaaaa attaggaaca aaagtaggaa aaagtaaagt gcatcatttt
     6241 gctgatggag aaatcttagt tgaaattgat gaatcagtac gtggtaagga tgtctttatt
     6301 gtccaatcta cttctaatcc ggtcactgaa aacttaatgg aaatcttagt tttagctgat
     6361 gctttaaaaa gagcttctgc taaagaaatt acagcaatca ttccatattt tggatatgct
     6421 cgtcaagatc gtaaggctaa accaagacaa ccaattacgt ctaaattagt agctgactta
     6481 ttaactgtag ctggtgtaac tagagttgta acagttgact tgcatgcagc tcaaattcaa
     6541 gggttctttg atattccagt tgatgaaatg caggcattgc cacttatctc aaactacttt
     6601 aagaagaaag atatggaaga tatttgcgtg gtttctcctg accatggtgg tgccactaga
     6661 gctcgtaaat tagcagtagc tttagatgcc ccagttgcaa ttattgataa aagaagacct
     6721 aaaccaaacg tggctgaaat catgggtgtt ttaggggatg tatccgggaa gaactgtatt
     6781 atggttgatg acatgatcga tactggagga actattgttg ctggtattga gatgttaaaa
     6841 gaaaaaggtg ctaaatcagt tcatgttgct tgtacgcacc cagtattctc tggacctgca
     6901 gttgaaagat tacaaaattc atctgctgat gaagttgttg taacagatac aatcaaatta
     6961 cctgaagata aaatgttccc taaattaaag acagtttctg tagcaggttt acttgctaaa
     7021 acaatcgaaa acattgaaaa ctgtttacca gtttctgatg tctttgaaat gtttgatttt
     7081 gataaataat attttgtgtt tcttaatgaa actagagtcg tctatttgga taaatttaaa
     7141 ggatataatt tttaagatta tatcctttta tcttttttta gttacaaatt attttagaaa
     7201 agatgatatt tacgcccaga aggaagatga taaaagtaag aaattcctag accaattaca
     7261 gcgattaaaa ataaaattaa gtaaaaatca gtataagaat aagtatcaag tatttttcca
     7321 ccaagacctt gaccaacaat tgaagaaaaa ctattgaatg tagaaaccca tgcaagagca
     7381 ctcatctgta aatcagaatc aactatagaa acaacgattt tcatattgag cataataaag
     7441 attacggttg atacagcctt agtaccgata gatataatca catgtatgat attataagga
     7501 ataaaacaat atgtaccaaa ctgaatgatt aataagataa aaacaattat taacatatga
     7561 cgatttgaaa tgttattcat aaaacgatga gaataataaa taattggtaa ttctgaaata
     7621 gtaatcaata aaataattgt agaaacctta tcaaccgaaa tcccttgatg ctgaaacatt
     7681 gctggtaaat aggtcgtatt taggttagtt atcgcataga ataagcaaac tataatcaaa
     7741 tagacgataa agtttttatt ccataagcct aggtgttgtg tagtcgtttc accatgaata
     7801 tcagaatttt tatgttcgtt tctagtaccg ataataccta gaagacaaat tcctaggcta
     7861 atagaaaaaa agaaatacat actttcaggt gaaatgtatt gatatataag accaccgatt
     7921 tgtgaaccaa tagcatatcc aattgttccc cagattctaa tttttccata ttgatattta
     7981 gataaggtgg ccattctttc aataactggc ttagtgctgt taaagagccc taaagtaaga
     8041 ctgtacaata aagtaatact aaaaagatgt tttgcaaaaa ttagagctat tcctaaaatt
     8101 gcggcaataa ttaaaacaac actatttacc cattttaagt taaagtgatc atttagatag
     8161 cctactagag gctgtaaaat tactgaaaca atgtatgaag atgctactac cagactaact
     8221 tgtgaagcac tatatccacg atctagcaga taaacagaaa tcaggcctga taataacgcc
     8281 agtgataaaa aatagaataa atacattaat aaataactta agtaactacc tcgatactgc
     8341 atttaaattg ccccctttaa taatataatc aaaatatcac agctatgaac gtgattaaat
     8401 atttaaatat tatttaggca taatgttcac aattaatagt caatatttat taaaatttgt
     8461 aaataatatt ctcattctat ataatgaaga tattaggagg taatgatgat gttgttaaaa
     8521 gaacaaatga aagcctaccc cttttcacat aatgaacggg taattattga ttatattcta
     8581 gataaacaga ttaatattaa agactattct gttaaaatga ttgctgatgc aacatttaca
     8641 tcaccatcaa ctttgattag aatttcgaag aagctgggtt ttcaaggctg ggttgaattt
     8701 aaagacgcct atttagaaga agcaaattat ttaaatagcc atttttgtaa tattgattct
     8761 aatttaccgt ttagtaacca agattctatt atgacaatag ctagtaaaat cgtaaattta
     8821 cacattgaaa gtgctaaaga tactttgtct ttgttccaac atgattcatt acaaaaagca
     8881 gtacgaattt tacaccaaag taaagagata cgggtatttg caatgtcaaa tctaaatttt
     8941 gctggagaag aatttgtatt taaattaaat cgaattcata aaaaagccta tattcaccct
     9001 atacaagata atctatttca tgatgctgca atgtcttcac ctgatgaatg tgctatttgt
     9061 atctcttatt caggtgagtc ttctaatatt gtgaagaccg ctcagatatt aaaagaaaat
     9121 aagtgtccaa ttatagctat tacgagtatt gggaataata gtctttcaga tttagctacg
     9181 gttactttgc gtgttacgac aagggaaaaa tcattttcaa aaattgctgg attttcatca
     9241 ttagaatcta tcagtatagt tttaaacacc ctatacgctt gtttattttc attaaattac
     9301 catgataatc tgacttataa acttgatatt gctaaaaaaa tagaaaatgc tcctattatt
     9361 aataatgaaa ttatagctga gaaagatgat gagtaaagtt aacacgatta gatatctaat
     9421 tctcttattg acattctaag tatatctgtt atgatatcaa agagccgacg gatacatatt
     9481 ccgcttaagt aattaagtgt cggggaatat gtaatgttga aaacacattc ttttaaggat
     9541 gtgtttttta tttcaaataa aactatacat aaaacgaata gtataaataa aatataagta
     9601 ttagacatag taatgcaatt gattataatg aaagtgtaat caaaggagga taattattat
     9661 ggataaaaaa atacttagat ttgccttatt atcagcatca ttattagtgg ggagtgctgc
     9721 agcgattaat gctaatattc cggccatggc acaacatttt gatcaagtac cattatcaat
     9781 ggtagaaatg ttaacgacga taccgtcatt atttttgatg atttccgttt taacaagttc
     9841 tttaattgct aaaagagttg ggtataagca aacaattact attgggctgg gaattgttat
     9901 gattgcaggg atagtaccgt tattaattga taattttatg attattttga ttagtcgtgc
     9961 aatgttggga tttggtgttg gtctgtttaa ttcactattg gtatcgatga tcaattattt
    10021 ttatgatgct aaagagcgtt catcaatgta tggattacaa tcagcatttg aaggggcagg
    10081 aggaattgcg attactttta tcgctggtca gttattaaag attnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn ncaggctccg tttattgctt atatgattgc aatacctgtt ttctttattt
    10201 attttaagtt cgtccctcag gtaaaaacag ccgatgtgat taaggctaat ggtggggatc
    10261 aaataaaaaa agaaagccat aaaagtgcag gctttttgcc agtggtttat tatgttggat
    10321 taatttttat ggctgcaatg ttatatatga tcatgggtat taagattgct tctttaatga
    10381 ctggtgaggg atatggaaca gcgagtgatg ctagtttagt aattatttta ttatcattag
    10441 gtggaattag cgcaggacta ttatttggaa aaatcttaaa ggtttttaat cagcttacta
    10501 catcgattgg attaattatt ttagcattag caatggtaat tttaggatta agtcaaaatt
    10561 tagttataac gtttgttggt ggatatttaa cggggtttgg ttttaaaatt tttatgccaa
    10621 gtttgattga taaaattaat aattcaaata ttccaaatac tacgttagct acttcattat
    10681 tattggttgg attcaattta ggggtgttta tctctcctta tggcagtatt gtaatccaaa
    10741 gtcttatgag aactgaagct ttaccagctt tatttattgc taatgctata ggatttatca
    10801 gtttaagtac aattacatta attattaccc ttataaaaaa taaaaaaaca gttaatatcg
    10861 ttcaaaaagt tttagaataa tagtattata gataaataac tcgtataata aaaagtggtg
    10921 ataaagatgg atctaagagt tctagaatat tttttggcag tagttgaatc aggtaatgtt
    10981 tctaaagcag cccaaaaact acacttgaca caaccgacgc taacaagaca attacagcag
    11041 ttagaagaaa tttatggcac acgtttgttt attcgtggga gtagacaaat tactttaact
    11101 aatagtggca taattttaaa acgtcgtgcg aaggaaatgc tagatttgca agataaaaca
    11161 gcagcagaaa ttagaaaaag tgagtatgat atttcgggag aaattaccat aggatgtggt
    11221 gaatcgagtg gaaataagtt tttaccagta gttttaaagg agttctcaaa acgttatccc
    11281 aaaattaaat ataatattta tacggctggt agtaatcaaa ataaggaaaa gatcaacgag
    11341 ggtttaattg atgtagcaat tgttttagaa ccaatcgata aagactatta ccaatacctg
    11401 gagttgcctt atttagataa atggtgcttg gtaatgaaaa aagatgattt attgaaagaa
    11461 tatgaaacaa ttgaaaatca cgtagtatct aatttagttc ttggagttgc aaatcgatta
    11521 gaagttaaag aaactttcaa aaactggtta ggtaaagaga taaatgcaca aacgatttta
    11581 aattatgata ttaattcaaa tttagcttta ttagtagaaa gtggtctttg ctatggaatc
    11641 tcgattgatg gagttttcaa aagtaatgct tatccaaatt taacgtaccg actattagaa
    11701 ccagaaattg tttcacaatc gtgtttgatt tggaaaaaag gggtagtgac taatcaagca
    11761 ttagagaaat ttattgtttg tgcgaaggaa ttgatttcat caaatcagta ataatataaa
    11821 agacatattc tttaatagga tatgtttttt atattttgtt gcattattga aaagagatga
    11881 ttagttaatg aaatcattgt tttttgcact ataagtaata aatgttgcat tactgcaacg
    11941 aaatttataa ttgtcgaaaa tcattccatg tgctaaattt ttaatatggg ttagttaagg
    12001 ctaactaaaa aaagagaaag gaaaaatgga cagtaaaatg ggaaaaaatt taaataaagc
    12061 actcatatcg gtgctttcat tgggaatgat tgtttcaatg tgctcgataa cggttaatgc
    12121 ggttgaaaca gataatgtgg ctaattgggt tacgatgtat ggagataatg gtaataatag
    12181 tattactcca gatacagtca atgatgctga aagactgatc aatggtgata ttattgcaac
    12241 aggaactttt gatggcaata aggtaacagg tattgaacag gctaaaggaa agagtgatgg
    12301 tgctgttatg ctatatgaca aaaatggcca aataaagtgg gaaactttaa taggtgggag
    12361 tgcagcagat agttttaatg ctgtaactga agctaatgat ggcagttatg ttgcagtagg
    12421 ggtaagccag tctaatgatg gtaatttagc caatcttaat aaggggggaa aagatggaat
    12481 aatcgctaag ctagattcaa atggaacttt aattaaggtt gttaccgttg ggggtagtga
    12541 tgcggatgaa ttgaaggcta ttcaaccagc tggcgatggt agttatttag cagctggtta
    12601 ttcgagttct agtgatggag atctcgaagg attagctcat ggtggtaaag atgcatttgt
    12661 tgttaagttt aatgaaaatg gaacaaaaga atgggtaaaa gcttttggtg gaactggaga
    12721 tgatgttttt aatcatataa ttagagctca taataaaaaa gtatctactg ataaaacaga
    12781 aatatcaagc agtgatattg ataatgggta tgtattaaca ggaacgacta actcaaatag
    12841 tggtattgtt ggaaatgaag aggaaaataa tgttgcattt accttaaaga ttgattcaaa
    12901 tggacaagtt gaatggacta caagccttgc taataatagt aagacaacag gagattatct
    12961 tttagcgatt agtgatggtt ttttagcggt tggaaccttt gatactaatg atcaagattt
    13021 tacagggata aagacatacg gtaaagaaga tatttatatt gcccactatt caaaggatgg
    13081 cacttgttta aatatttcaa gctatggtgg agatggaaaa gataatccag aaggaattat
    13141 tcctggagca aatgatgact atatcgtcta tggtaataca aattctaata gtggcttgtt
    13201 tgaaggaaaa ttaaaaggta agtatgaagg ctttttagct agtttagcgg gaacaacact
    13261 agagcaatat gcagaagaaa agtatttagt gccggtagaa gcttggaaag ctaatgaaga
    13321 tagtctttca atgatggcac cattattata taaagatgca tatgttgaaa aaattggctt
    13381 taagtatact gtaacaattt attttactaa cgcaacaatt atgggaactc aagttagcgc
    13441 ttcaacatta ggggaagtta gttatgaaaa aaatggtgaa atgatagcgg tgttgaaaga
    13501 tgagtacgat gaagtaacac aagtaaaaac agttacgctt gaagttgatg atttagatac
    13561 accggtcaaa ttacatatcg ataaagcaat gggagatata cgtttatctt tctctagtaa
    13621 agatatggta gaaactacca atccgccata ttttgctcca gttgaagtag aacagccaga
    13681 ctttaaaact agttggaaag taaatattgg tggtagtgat tatgattata ctgatgatat
    13741 gacagttttg aaaaatggaa atattgttgc tgtagggcaa tcatactcaa atgatggtga
    13801 ttttcaaaat ctgttaaaag gtggttcaat tgcttatatt aatcaatatg atccaaaagg
    13861 agaactttta aaaactctta ctttaggagg aacagagtac gactcaattg catatgcagc
    13921 taaggtatgt ggcctagatg atggtggttt tgtagttagt ggtggatatc aagaaggggt
    13981 gtatgtggct ccaaccggtg attttgcaca gttagatact gctggtaccg ttcatggtca
    14041 aatggatact tttattgcta aatatgactc taacagtaag ctgatctgga tgagtaattt
    14101 tagtggctca gccaacgatc aagttaaaca aattaaagct acggatgatg gcggtattgc
    14161 tgttttaata gaaacaaatt caaatgatgg cgatatggaa aatcaaaatc gaggattata
    14221 tgacttagtc atagttaaat atgatgctca tggtaaaaaa gtctggcaac gagttttagg
    14281 tggaaaaaat attgaaac
//
LOCUS       DAOMTA010000004        13474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_15_length_13474_cov_2.585811, whole genome shotgun sequence.
ACCESSION   DAOMTA010000004 DAOMTA010000000
VERSION     DAOMTA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_13474_cov_2.585811"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PLK68_00250"
     CDS             <1..1135
                     /locus_tag="PLK68_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88870.1"
                     /translation="EIMKSIAGIIGYGSKTYKYFPTWSSIILEILLSVVALFIFALTA
                     IQIAMRSVICSMKYLLAPYMVASIIDPEDNSFGTWLRSIGGDLIANFCQIYFTYFVLI
                     LCSNSSIQNALGNDWIGIFSKIALFIGGLLAIQEIPNFISQFFGSSGQSMMETMRGTM
                     MMGSMLMMTGGAAAKTVGMGIGAGKFAYQTGKAMTASYNEAGGGMNGLKSAGGEFIGG
                     VFGKGGSHSNTNNINEEGTSQNNNHTNFREEHKESSNHSQGEQTDNNNVNSTNPNNQK
                     MNSFNDIGKEVLNPLNDDIGGKTYISPEYQGNEDYDSYLNAAKYYGIENPESMSKDEL
                     RAALNQKGYKYTSMKNFRGSFNNESYKTNIKSIKEKNKKGKKK"
     gene            1565..2023
                     /locus_tag="PLK68_00255"
     CDS             1565..2023
                     /locus_tag="PLK68_00255"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM88871.1"
                     /translation="MDEIIRLQKYLSLLRTTANWSVQEFADMLGVTRQTINNLEKKDP
                     IKMSKVQYLAIRSLFTHEIENGNEVLGKLLYCLVDHPERITENDRAFIANQAKILSSA
                     AKGGSTKEDIDESWAQIFGMIGGIVGAAIIPFAIEAGIEWLDVIMNKSKK"
     gene            2042..2281
                     /locus_tag="PLK68_00260"
     CDS             2042..2281
                     /locus_tag="PLK68_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88872.1"
                     /translation="MNNELVKMSNEIIMLDLDDTENPVKCVNVIESHDRKRILNNIIQ
                     YVDIANLVKNIDSRTEYIVQFPLKALDDFKSGKLL"
     gene            2320..>3037
                     /locus_tag="PLK68_00265"
     CDS             2320..>3037
                     /locus_tag="PLK68_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88873.1"
                     /translation="MKKGKDGRYHIAHNLPIKEETIVSGASLQDFAVIQHNLYVQQQL
                     NEISNQMTHIIEVVERIENGQKTDRIGILNAGKKQIMYALNIENTEDRKTAIMAGVDN
                     LILAQQQILAAMKSRIEEFEPLSSNSVVRFINGVFKDEYLNRKYDDYMDIQEYYALYL
                     HATEMIASAYIILGNEANVHEVFVDAKTELNKISFKNIKTLEYVLNSKELFFNNIVQN
                     LKHEENLALEYAREIDCLRIS"
     assembly_gap    3038..3047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3048..3252
                     /locus_tag="PLK68_00270"
     CDS             <3048..3252
                     /locus_tag="PLK68_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88874.1"
                     /translation="MNYWRRAIMDDKKEFKFTKPNKEKLNKEANAAKVVKNGGKIFGL
                     AAIFGAGIKKYGPQLLDAIRNLK"
     gene            3373..3522
                     /locus_tag="PLK68_00275"
     CDS             3373..3522
                     /locus_tag="PLK68_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88875.1"
                     /translation="MSCYIDGTFVNERYFWASLQSLANQGQIARILEGQKVKVAESVY
                     WIERN"
     gene            3526..3876
                     /locus_tag="PLK68_00280"
     CDS             3526..3876
                     /locus_tag="PLK68_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88876.1"
                     /translation="MEKEFYETKITDKLIYMTLIRGYGFSFDYKDHHYDLVHFDADIE
                     DNPIPEPNTWLFDNGVCPPKWQLKYVEQLRYLHVGDECILDIIKSIDHDRLIVGTTFD
                     SLMRDASNKYQQSK"
     gene            3931..4596
                     /locus_tag="PLK68_00285"
     CDS             3931..4596
                     /locus_tag="PLK68_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88877.1"
                     /translation="MNKEKRLLKEQKKKEKQLKKKKQKRIKELKNILDWMHIQAIDEY
                     GIYLSKGNKDMVVRGLVLSSNNIFLKESYAKARDVFALSSAFDRLKGVTYFKFVKTPI
                     RLSSNINRLSEDLEIEKNPGRRKLIKISIDKMDDAMQVRKEMAFYALVQDKPEVIDKR
                     LYALKQEMNLAGLHPQVMGITDYEAVVAQQFENEIVNEYMYSSLIVDQEVYQNAMDQV
                     KTS"
     gene            4568..6430
                     /locus_tag="PLK68_00290"
     CDS             4568..6430
                     /locus_tag="PLK68_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88878.1"
                     /translation="MRWTKSKRARRTTIAPKGLLERNNYYILGDTYVRVLTISALPNE
                     FGEGFLAQFINDHRFHIDISTSIMNEDVSSFLQKEINELESKIDKCDNDINREILIKR
                     YRSYQDSLKQLVANNSRTINTVINLYIHAKSLQDLEEYTKELKEFFYSTGLSVRLDTL
                     PRLQVGTMQKNSPLFIGNSLDRYSNYHIGLMMPSLSVAGLWPFIFDSMDDKYGSFIGV
                     EANTGGKIVFDQFAYINDKNARYQGRTAGNMIIVGRTGMGKTTIINLLVNSHLINRRK
                     VIWCDPENKNKKLTNELGGNYIEFGVDKNIINIFDLRPVTTDDENREDDSVMYDTITA
                     ISNVVEDLTITFRLLWPTISENELDMVNEITVKTYRSAGIDGSESFKYYTPKDYPTFT
                     DFSGTIDELIDEYSKNLSLYKREYDALKDLQMRMRNLVGTPDVPGQNARYFNGKTNID
                     FEALEDIGLLSFGMKHLSSTPIGVRNALLRLVFNYAWSKCLSTDEETVFVSDEDHEFI
                     SIEEIASIKAQFQRRARKYNTVTISGTQQVRDYCDEKILSYGRAIFENSTYQMYFNMY
                     KSSVEDLSQLIVLTDQEKEQLVNLPPYTCLTEVGNRKIPIRILLTDEEDRLIGK"
     gene            6431..7603
                     /locus_tag="PLK68_00295"
     CDS             6431..7603
                     /locus_tag="PLK68_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HRM88879.1"
                     /translation="MNTLKYLMSKMTRKVGVTLLVFILSMGAILTPFVAMNEYLKHPV
                     SITYDIIFGDVFKIELGGNINVKVLQYKTEIEKWTSKEKNGYYDLTPYENVLFALMMT
                     ESGGNGNDPMQASECGKNTRYSHKPNSITNPKYSIECGVKNFADAMARAVSKGLSGSD
                     ALYAAIDGYNKGFGVISKHGEVGHYSLETSCKFCYDHRFSDQKKSYSTAATLKSQYNL
                     KSNNYWDGGSWRWNYGNMFYVYKVLSYLKISDDTAIPYDGTLGSMIAAEAQKKIGCRY
                     WYGEEGPNYFDCSGLVYWCCKQAGISITREGANTYSKMGKAIAYKDLKPGDVVTFDWD
                     NDNKAEHIGIYIGGGFMIHAHGDSYVHGNYAKFVVEKKNISSGYYHDHIFRCRRLY"
     gene            7814..9211
                     /locus_tag="PLK68_00300"
     CDS             7814..9211
                     /locus_tag="PLK68_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM88880.1"
                     /translation="MKELDLGSEKLGSLVRRFSIPCVISMVVAALYNIVDQIFIGWSE
                     AGAFGNAATNIVYPFTVLALGLALLIGDGAAAGFSISLGQGNKKQSNKNIGNGFTLLI
                     IISLILCIIGFICRVQILGLFGGNPNEVECYGYATDYYKIICIGMPFYMIGQGLNGAI
                     RADGSPKFAMGCTLVGAITNLVFDPLLIFGFNMGVKGAAIATILGQIITFLMSIFYLT
                     RAKNFKINKEGMTLDKTTVMKIISVGMASLIVQLSIVVIIAVNNNLLTKYGYETFAST
                     GVAYGAVTPLAVVGIVMKVFGIVVSIVIGISLGGQPIIGFNMGAGNMERVKETIQLIT
                     QLVIGVGVLSFLLFEFAPDVIIFLFGSHNTPEYMEYARLCIRIFLGGIILTCYIKSAA
                     IILQSMGNSMKSTVLALLRDVIIFVPASIIIASITRSIVTMLWAAIISDIIAAIIGFI
                     LVQSEIKKCEKLIDR"
     gene            9408..10313
                     /locus_tag="PLK68_00305"
     CDS             9408..10313
                     /locus_tag="PLK68_00305"
                     /inference="COORDINATES: protein motif:HMM:NF039828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HRM88881.1"
                     /translation="MSNLSAYRNYVNHVAEIMNDQVDYTVDATWVEKPNDTYYGLRFL
                     VDENMGLCFDMHQEYEDYQRGKGISQTVKFLKEVVNNEIKKMNAFEEYDLLENLSNYD
                     FIKERLVVEAVNFSNNEEFLEFVPHQTIGDVSCVYRVVINDFSDEEERASFVIRNDML
                     KQWHISEDQLHYDAVNNSMKVEPITIKEFDTMGLPEAPFKICTTPNQMNGSGVIFYEN
                     FSEVVEQHGLGDCYIIPSSVHELLLCPKSKGFDPDALTDMLVEVNAAQVDIKEQLANH
                     IFEYDSKTKEVIPLFEESLDMSLAA"
     gene            10444..10893
                     /locus_tag="PLK68_00310"
     CDS             10444..10893
                     /locus_tag="PLK68_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88882.1"
                     /translation="MNRSMEDTIMAFTEKYEGDFRRIYQAIATKERLTDEEIGQYIDD
                     IEEPALNIIAPDYPQSLKEIDCPPFVLFFEGNPEVIHDNVFCLANPYDKDKRVFFSIE
                     FTDEGTDYFLGCENRNDLEPLLDFFIEQHPELNFMDYKAKEGEAVCL"
     gene            10984..12279
                     /locus_tag="PLK68_00315"
     CDS             10984..12279
                     /locus_tag="PLK68_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRM88883.1"
                     /translation="MKQNKAREEIVKLFVDCLEKEDLPWHQGFIPSRTSFNPITNTTY
                     KNSNRFILYLNEYIHQYDDPRWMTFKQASEKGYHIHKGEKGVPIEYWSIYDTKEKKKL
                     TFSQLHDLLNKEPDRKDDIRYISQVYTVFNAKQMDGIEPYKENKAIIKDEIDEQFSLP
                     YYLLYEFAHETGLDLNEDHKVNTPFYNLNDDKVVVPDHHRYKLTDAFFSDAFHEVSHS
                     TAHQSRLNRSIKYFMEDREAYAIEELRAEIGSSFICNDLGIIPQKNEDYLENSQAYIQ
                     SWLKAIKEKPNTLFAAIKDAESICDYILNKGHYERLVKIETSINEAIVDGSYKENAIS
                     KDELRMILRNGYQEVDPKEMTTLVDDWSNQKEVEPKIYYTFDGEKIVCADNTSNDMFM
                     EEFNKEDYLLAFLWMAKGMAMDEVMILKFHDSKDVVAVK"
     gene            12280..12486
                     /locus_tag="PLK68_00320"
     CDS             12280..12486
                     /locus_tag="PLK68_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88884.1"
                     /translation="MIIIAGTRIKYNKVKYPIKVGTKSYGWTMVASYTHHNVWERITR
                     SGVPIRTSFFKDEVPNENATYTWD"
     gene            12643..12963
                     /locus_tag="PLK68_00325"
     CDS             12643..12963
                     /locus_tag="PLK68_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88885.1"
                     /translation="MANDGNLSIRCTNHTKEMFNAIAKKNAHLSKGELLEEMINRYQA
                     VETINNDAINEVLKLTQLNHEYIAFMMKCNKSIPTNVATVIKLINEEAMCEKYIRMKL
                     RKEI"
     gene            12996..13271
                     /locus_tag="PLK68_00330"
     CDS             12996..13271
                     /locus_tag="PLK68_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88886.1"
                     /translation="MNIRKAYWVSKDFDKKLQEMQEYMKDVCHLDYTVADLFDEMMSL
                     YEARIKFNANILNELFREHDEAMLDQFLISNAEMLNSLKEYIEGVTR"
     gene            13290..>13474
                     /locus_tag="PLK68_00335"
     CDS             13290..>13474
                     /locus_tag="PLK68_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88887.1"
                     /translation="MKITKIRFFEYGQKPPKNSRFKDTISNETVGGYFDYTSREEAKD
                     VDKEIVKQEDGYFGYTG"
BASE COUNT         4951 a         1659 c         2611 g         4243 t
ORIGIN      
        1 agaaattatg aaaagtattg ctggtattat tggctatgga agcaagactt acaagtactt
       61 tcctacctgg tcctctatta tattagagat ccttttatca gtagttgcac tgtttatttt
      121 tgcattaaca gcaatacaga tagcaatgcg gtctgtgata tgttcaatga aatatctttt
      181 agcaccttat atggtagctt caattattga tccagaagat aacagttttg gtacatggct
      241 aagaagtatt ggaggagatt tgattgccaa cttctgtcaa atctatttta catactttgt
      301 attgatttta tgcagtaatt catctattca gaatgcttta ggtaatgact ggataggcat
      361 cttttctaag atagctttat ttattggtgg attacttgct atccaggaaa taccaaactt
      421 catcagtcag ttctttggaa gttcaggtca gagtatgatg gaaactatga gagggacaat
      481 gatgatgggt tctatgttga tgatgacagg tggagctgca gcaaaaactg taggcatggg
      541 aattggtgca ggtaaatttg cctaccagac aggtaaggcg atgactgctt catacaatga
      601 agcaggtgga ggcatgaatg gtcttaaaag tgcgggtggt gaatttattg gtggtgtatt
      661 tggaaaaggt ggttctcatt caaatacaaa caatatcaat gaggaaggca cttcacaaaa
      721 taacaatcat acaaacttta gagaagaaca taaagaatca tctaaccata gtcaaggtga
      781 acaaactgat aacaataatg taaattctac aaatccaaac aatcagaaga tgaattcatt
      841 taatgatatt ggtaaagaag tgctgaatcc attaaatgat gatattggtg gtaaaacata
      901 catttcacct gaatatcagg gtaatgagga ttatgacagt tatttaaatg ctgctaagta
      961 ttatggaatt gaaaatcctg agtcaatgag taaagatgaa cttagagcag ctttaaatca
     1021 aaaaggatat aagtatactt caatgaagaa ttttagagga tcatttaata acgaatccta
     1081 taaaacaaac attaagagta ttaaagagaa gaataagaaa ggaaagaaga aataatgcaa
     1141 ttctattgtc caagagatta taaaaatgga aagtattact tcaacaagta tgatgcattt
     1201 gatctgatgg taatggtacc cctagtagca attggtacat ttctaagcgt catttcatta
     1261 tctatgatgt cacaaggtaa ttttaccttc tcagtcatag gattaacttt aggattatta
     1321 attgtaggta ctacattaat tctatttatg ccttataaga tctatcacaa tatcttaaac
     1381 agactcattg tggaaataag gtttcagtta agacagaaag acttcttatg gtatggatat
     1441 gactttaaca actatgaaga ttaataaaaa catttagtat gataaaattc agctcgatat
     1501 atttaatgta aaaatatttg acataagtgg tataatgtat tatattttta tcgaggtgaa
     1561 atatatggac gaaattatca gattacaaaa atatttatca ctattgcgta ctactgcaaa
     1621 ttggtcagta caagaatttg cagatatgtt aggtgtaaca cgtcaaacaa ttaataatct
     1681 tgagaaaaaa gatccaatta aaatgtctaa ggtacagtat ttagctatac gcagcttatt
     1741 tactcatgaa attgaaaatg gtaatgaagt gttaggtaaa ttgttgtatt gcttagtaga
     1801 tcaccctgaa agaattacag aaaatgatag agcatttatt gctaaccaag caaagatatt
     1861 atcatctgca gcaaaaggtg gctcaacaaa agaagatata gatgaaagtt gggcacaaat
     1921 atttggaatg attggtggca tagtaggagc tgctattatt ccttttgcta tagaagcagg
     1981 aattgaatgg ttagatgtta ttatgaataa gtctaaaaag taatcgaagg gtggtatctt
     2041 aatgaataat gaacttgtta aaatgtccaa tgaaattatc atgttggatt tagacgatac
     2101 agaaaatcca gtaaaatgtg taaacgttat agaatcgcat gatagaaaaa gaattcttaa
     2161 caatataatt caatatgtgg atattgctaa tttagtaaag aatatagatt ctagaacaga
     2221 atatatagtg cagtttccat taaaggcact agatgatttc aagtctggga agcttttata
     2281 aatgttaatt caaagactgg agtcagttgg ccacaattaa tgaaaaaagg gaaagatggg
     2341 agatatcata tagcccataa tcttcctata aaagaagaaa cgattgttag tggtgcgtct
     2401 ttgcaggact ttgcagttat ccaacataac ttatatgtgc agcaacaatt aaatgaaatt
     2461 tcaaatcaaa tgactcatat tattgaagtg gttgaaagaa tagaaaacgg tcagaaaaca
     2521 gaccgaattg gtatcttaaa tgcaggcaag aagcaaataa tgtatgcttt aaacattgaa
     2581 aatacagaag atagaaaaac agccattatg gcaggggtgg ataatttaat tcttgctcaa
     2641 caacaaatac tagctgctat gaagtcacga attgaagaat ttgaaccact ttcatctaac
     2701 tcggtagttc gatttattaa tggggtcttt aaagacgaat acttgaaccg aaagtatgat
     2761 gattatatgg atattcaaga gtactatgcg ttatacttac atgctactga aatgattgct
     2821 tcagcttaca ttattctagg taacgaagcg aatgttcacg aggtatttgt agatgcaaaa
     2881 accgaattga ataaaattag cttcaagaac ataaaaactt tagaatatgt actgaacagc
     2941 aaagaattat tctttaataa tatagttcaa aatttgaagc atgaagaaaa tttagctcta
     3001 gaatatgcta gagaaattga ttgtttaaga atttctgnnn nnnnnnnaat gaactattgg
     3061 aggcgtgcta taatggatga taaaaaggaa ttcaagttta ctaagccaaa taaggaaaaa
     3121 cttaataaag aagccaatgc tgctaaggta gtaaaaaatg gtgggaaaat cttcggccta
     3181 gctgctattt ttggcgctgg tatcaagaag tatggcccac agcttctcga tgctattaga
     3241 aatctcaagt agattaatgt aaatttgcaa tccccaatgg attgcttttc tatttgagta
     3301 attgattcat caaatagctc atttgcacaa aactcatatc atgttataat caatatccgg
     3361 agagtgataa cgatgagttg ttatattgat ggaacatttg ttaatgaaag atacttttgg
     3421 gcttctttgc agtcactagc taatcaaggt caaatagcta gaatactaga aggacaaaaa
     3481 gtgaaggtgg ctgaatctgt atactggatt gaaaggaatt aaatgatgga aaaggaattc
     3541 tatgaaacta agattacaga taaattaatc tatatgactt taatcagagg atatgggttc
     3601 tcatttgatt ataaagatca ccattatgac ttagttcatt ttgatgctga tattgaagat
     3661 aatccaattc cagaacctaa tacctggttg tttgataatg gtgtgtgccc acctaaatgg
     3721 cagcttaagt atgttgaaca gttaagatat ctacatgttg gtgatgaatg tattcttgat
     3781 attattaaaa gtattgatca tgatagatta atagtaggta caacctttga ttctctaatg
     3841 agagatgcct caaataagta tcaacaaagt aaatagtatt tataaaacag ctttggctgt
     3901 ttttttcttt ttgcctggaa ggaggattta atgaataaag aaaaacgatt attaaaggaa
     3961 caaaagaaaa aggaaaaaca acttaaaaag aagaaacaaa aacgtattaa agaattaaag
     4021 aacattttag attggatgca catacaagct attgatgaat atggtatcta cttatccaaa
     4081 ggtaataaag atatggttgt taggggatta gttttatctt ccaacaacat ttttcttaaa
     4141 gaaagttacg caaaggctag agatgtattt gcattatctt cagcttttga caggttaaaa
     4201 ggtgtgactt attttaaatt tgttaagaca ccaatcagac ttagcagtaa tatcaaccga
     4261 ttaagtgaag atcttgaaat agagaaaaac ccaggtagaa gaaagctaat taaaatctca
     4321 atagataaaa tggatgatgc aatgcaagta cgtaaagaga tggcatttta tgcattagtg
     4381 caagataaac cggaagtcat tgataaaagg ttatatgcat taaaacagga aatgaatctt
     4441 gctggattac atccacaggt aatgggtatt acagactatg aagctgtagt ggctcaacag
     4501 tttgaaaatg aaatagtcaa tgaatatatg tatagcagtt taatcgtaga tcaggaggtg
     4561 tatcaaaatg cgatggacca agtcaaaacg agctagaaga acaacgatag ctcctaaagg
     4621 attattagaa agaaataact attatatttt aggtgatact tatgtaagag tattgactat
     4681 ttcagcactt cctaatgaat ttggtgaagg ctttttagct cagtttatca atgatcatcg
     4741 tttccatata gatatttcta caagtattat gaatgaagat gtttcttcat tcttacaaaa
     4801 ggaaattaat gaattagaaa gcaagattga taaatgtgat aacgatatta atagagaaat
     4861 cttaattaaa agatacagat catatcagga ttccttaaaa cagttggttg caaataattc
     4921 gagaacaatc aatactgtaa ttaacttata tattcatgct aagtcattac aggatttaga
     4981 agaatatact aaggaactta aggaattctt ttattcaaca ggactgtccg ttcgcttgga
     5041 tactttacct cgattacagg tgggtacaat gcagaagaac agtcctttat ttattgggaa
     5101 ttctttagat cgatacagta actatcatat tggattaatg atgccttcgt tgtctgttgc
     5161 agggttatgg ccatttattt ttgattctat ggatgataaa tatggttcat ttattggtgt
     5221 tgaagctaat acaggaggaa agattgtctt tgatcagttt gcctatatta atgataaaaa
     5281 cgcaagatat cagggtagaa cagcaggtaa tatgattatt gttggtagaa ccgggatggg
     5341 taaaactacc attattaatc ttttagttaa ttcccattta attaatagaa gaaaggtcat
     5401 ctggtgtgat ccggaaaata agaataagaa actaacaaat gaattaggtg ggaactacat
     5461 tgaatttggt gttgataaaa acatcatcaa tatttttgat ttaagaccag taacaacaga
     5521 tgatgaaaac agagaagatg acagtgtcat gtatgatacg attacagcta tcagtaatgt
     5581 cgttgaagat ttaacgatta ctttcagatt gctatggcca acaatttcag aaaatgaact
     5641 agatatggta aatgaaatta cagttaagac atatagaagt gctggtattg atggcagtga
     5701 gtcgtttaag tattatacac caaaggatta tccaacattt actgactttt caggtacgat
     5761 agatgagtta attgatgaat acagtaagaa cttatcatta tataaaagag agtatgatgc
     5821 attaaaagat ctacagatga gaatgagaaa tcttgtaggg acacctgatg ttccaggaca
     5881 aaatgctaga tattttaatg gaaagacaaa tattgatttt gaggcattag aagatattgg
     5941 cttattatca tttggtatga aacatctatc atcaacacct attggtgtaa gaaatgcatt
     6001 attaagactt gtatttaact atgcctggtc taaatgctta tctactgatg aagaaacagt
     6061 atttgtatcg gatgaagatc atgaattcat ttctattgaa gaaattgctt ctatcaaagc
     6121 tcagtttcaa cgacgagcaa gaaagtacaa tactgtcaca atctcaggaa cacagcaggt
     6181 aagagactat tgtgatgaaa agattctttc ttatggtcga gcaatctttg aaaacagtac
     6241 ttatcagatg tatttcaata tgtataaaag ttcagtagaa gacttgtcac agttaatcgt
     6301 attaacagat caggaaaagg aacagctggt aaatttacca ccttatacat gtttaacaga
     6361 agttggcaat cgtaagattc ctattcgtat attgcttaca gatgaagaag acagattaat
     6421 aggtaaatag atgaatacat taaagtattt gatgagtaaa atgacgagaa aagttggagt
     6481 gactcttctc gtttttattt tatcaatggg tgctatatta acaccatttg tcgctatgaa
     6541 tgaatatctc aaacatccag tatcaataac atatgatatt atctttggtg atgtatttaa
     6601 gattgaacta ggtggaaata tcaatgttaa agtacttcag tataaaacag agatagagaa
     6661 atggacatca aaggaaaaga atggttatta tgatttaaca ccatatgaga atgtattatt
     6721 tgcattaatg atgacagaaa gtggtggtaa tggtaatgat ccgatgcagg caagtgaatg
     6781 cggtaagaat accagatatt ctcataagcc taattccatt acaaatccta agtattccat
     6841 tgaatgtggt gtaaagaatt ttgctgatgc tatggcaaga gctgtttcaa aaggactatc
     6901 aggaagtgat gccttatatg ctgcaattga tggctataat aaaggctttg gtgttatcag
     6961 caaacatgga gaagtgggac attattcttt agaaacaagc tgtaagtttt gttatgacca
     7021 tagattcagt gatcaaaaga aatcatacag tacagcagct acattaaaaa gtcagtataa
     7081 tttaaagtca aacaattact gggatggtgg cagctggaga tggaattatg gcaatatgtt
     7141 ttatgtttat aaagtactaa gttatcttaa aatatcggat gatacagcca ttccttatga
     7201 tggaacatta ggtagtatga ttgctgctga agcacaaaag aaaataggat gtcgatactg
     7261 gtatggtgaa gaaggtccta attattttga ttgttcaggt ttagtctact ggtgttgtaa
     7321 acaagctgga ataagtatta ccagagaagg tgctaatact tattcaaaga tgggtaaagc
     7381 tattgcttat aaagatttaa aacctggtga tgttgttaca tttgattggg ataatgataa
     7441 taaagctgag catataggaa tctatatagg tggaggtttt atgattcatg cccatggtga
     7501 tagctatgta catggcaatt atgccaagtt tgtagttgaa aagaaaaata ttagttcagg
     7561 gtactatcat gatcatatct ttagatgcag aagactttat tagtgaaaaa tgatataatt
     7621 agctagtaaa taagaaagac aaattagaat ttatagaaat actcattgct gatgtttcaa
     7681 tttcattaaa aaaaacttac gaaaaaaatt gtaaaatgtt aaaatacctt tcgtaatgag
     7741 cagcaagcaa gcgtaagtcc aattatggat ttacgttttt ttgttgcttt ttttacagaa
     7801 aggatgtgtt tttatgaaag aattagattt aggatcagaa aaattgggaa gccttgtaag
     7861 aagattttca attccatgtg ttataagcat ggtggtagct gctttataca atattgttga
     7921 tcaaatattt attggttgga gtgaagctgg agcgtttgga aatgctgcaa caaacattgt
     7981 atatccattt actgtgttgg cactagggtt agctttattg ataggtgatg gtgctgcggc
     8041 aggatttagc atttcattag gacaaggaaa caagaaacag tcaaataaga atattggcaa
     8101 tggttttaca cttttaatta ttatctctct aatactttgt attataggat ttatttgtag
     8161 agttcagatt ctaggattat ttggtggaaa tcctaatgaa gttgaatgtt atggttatgc
     8221 aactgattat tataagatta tttgtatagg aatgcctttt tatatgattg gacagggttt
     8281 gaatggtgca ataagagctg atggttcccc aaaatttgcg atgggctgta ctcttgtagg
     8341 tgcaatcaca aacttagtct ttgatccatt attaatattt ggttttaata tgggagtaaa
     8401 aggtgccgca attgcaacaa ttttagggca aatcattacc ttcttaatga gcatttttta
     8461 tcttacaaga gctaaaaatt ttaagatcaa taaagaagga atgacattag acaaaacaac
     8521 agtaatgaaa attatctctg taggaatggc atcattaatt gtccaacttt ctattgtagt
     8581 tatcattgct gtaaataata atctattaac taaatatggt tatgaaactt ttgcgagtac
     8641 aggcgtagct tatggtgctg tgacaccact tgctgtcgtt ggcatcgtta tgaaagtgtt
     8701 tggaattgtc gtttcaattg tcataggaat cagtctaggt ggacagccga ttattggatt
     8761 taatatggga gctggcaata tggagcgtgt aaaagaaaca attcagctta taactcaatt
     8821 agtcattggt gtaggtgtac tttcgttttt actttttgaa tttgcaccag atgtaatcat
     8881 cttcttattt ggaagtcaca atactcctga atatatggaa tatgcaagat tatgtattcg
     8941 tatattcctt ggtgggataa ttcttacatg ttatataaaa tcggctgcaa ttattcttca
     9001 atctatgggt aatagtatga aatcaacagt actagcttta cttagagatg tcattatatt
     9061 tgtacctgca tctattatta ttgcatccat tacaagaagc attgtaacaa tgctttgggc
     9121 tgcaattatt tctgatatta tagctgctat tatcggattt atattagtac agtcagagat
     9181 aaagaaatgt gaaaaattga ttgatagata aacaaattcc agtttgtcga atatattatt
     9241 caaattaaat attagtgatt aaggtattga ttagtttcaa tgccttttta ttttggaagg
     9301 agaaaagatg aatattgata ttatgattga agatttagtg attgaatctt ttgaatttaa
     9361 agaaggcaat attgctgaaa agtatatgaa tatgaaggag gaagagaatg agtaatttaa
     9421 gtgcttatcg taattatgta aatcatgttg ctgaaatcat gaatgatcaa gttgattata
     9481 ctgttgatgc aacctgggtt gaaaagccta atgatactta ttatggatta agatttctag
     9541 tagatgaaaa tatgggatta tgctttgata tgcatcagga atatgaagat tatcaaagag
     9601 gaaaaggtat tagtcagaca gttaaatttt taaaagaagt agtaaataat gaaataaaaa
     9661 agatgaatgc ttttgaagaa tatgatttat tagagaatct aagtaattat gatttcatta
     9721 aagaaagatt ggttgtagaa gctgttaact tttcgaataa tgaagaattc ttagaatttg
     9781 taccgcatca gacaataggt gatgtttcct gtgtatatcg agtggttatt aatgatttca
     9841 gtgatgaaga agaaagagca agctttgtta ttagaaatga tatgcttaaa caatggcata
     9901 tttcagagga ccagttacat tatgatgcag taaataattc tatgaaagta gaaccaataa
     9961 caattaaaga atttgatacg atgggattac ctgaggcacc gtttaagatt tgtacaacac
    10021 caaatcaaat gaatggctca ggtgttatat tttatgagaa cttttctgaa gtcgtagaac
    10081 aacatggatt aggtgactgt tatatcattc catcatcagt tcatgaatta ctgctttgtc
    10141 ctaaaagtaa agggtttgat cctgatgcat tgaccgacat gttagtcgaa gttaatgcgg
    10201 cgcaggtaga tattaaagag caactggcca atcatatctt tgaatatgac agtaaaacaa
    10261 aagaagtgat tcctttattt gaagaatcac tagatatgtc attagcagcg taaatattaa
    10321 acggaggaaa aaaaatggaa gatttaaaag aaaaatttgg tttaccaatc catatatata
    10381 atgaggggat tacctatgat gatgtaatcc tagcacttca acagtataag gaggaaacac
    10441 aagatgaata gatccatgga agataccatt atggcattta ctgaaaagta tgaaggtgat
    10501 tttagaagaa tctatcaggc gattgctact aaagaaagat taacagatga agaaattggt
    10561 cagtatattg atgatattga agaacctgca ttaaatatca tcgcacctga ttatccacaa
    10621 tcattaaaag aaattgactg tccaccattt gttttattct ttgaaggcaa tccagaagtt
    10681 attcatgata atgtcttttg tttagctaat ccctatgaca aagataaaag agtattcttc
    10741 agtattgagt ttacggatga aggaacagat tacttcttag gatgtgaaaa cagaaatgac
    10801 ttagaaccgt tactagattt ctttattgaa cagcatccag agcttaactt tatggattat
    10861 aaagccaagg aaggtgaagc agtatgcctt tagaaatact agatcagttt gattttgatg
    10921 aagtcaatcc atatgacaat gaagaaagtg aagatactgt ttatatgtta gaggaacttg
    10981 cagatgaaac aaaataaagc cagagaagag attgtaaagc tatttgttga ctgtcttgaa
    11041 aaggaagatt taccttggca tcaggggttt attccatcaa gaacatcatt caatcctatt
    11101 acaaatacaa cctataaaaa cagtaatcga ttcattcttt atttaaatga gtatattcat
    11161 cagtacgatg atccaagatg gatgacattc aagcaggcat ctgaaaaagg ataccatatt
    11221 cataaaggtg aaaagggtgt acccattgaa tactggagca tttatgatac caaagaaaag
    11281 aagaaactaa ctttttcaca gttacatgat ttattaaata aagagcctga tagaaaagat
    11341 gatatcagat acatctcaca ggtttataca gtatttaatg ctaaacagat ggatggtata
    11401 gaaccctata aagagaataa agctattatt aaagatgaaa tagatgaaca gttttcatta
    11461 ccatattatc tgctttatga atttgctcat gaaactggac tagatttaaa tgaagatcat
    11521 aaagttaata caccattcta taatctaaat gatgacaagg tcgtagttcc tgatcatcat
    11581 cgatataaat taactgatgc attctttagt gatgcatttc atgaagtatc acattcaaca
    11641 gcacatcaaa gtagattaaa cagatcaatt aaatacttca tggaagatcg agaagcatat
    11701 gcgattgaag aactaagagc tgaaattggc tcttctttta tttgtaatga tttagggatt
    11761 attcctcaaa agaatgaaga ctatttagaa aacagtcagg catatattca aagctggtta
    11821 aaagcaatta aagaaaagcc taatacattg tttgcagcaa tcaaggatgc tgaaagtatc
    11881 tgtgattata ttttgaataa aggacattat gaacgactgg taaagataga aacatctatt
    11941 aatgaagcta ttgttgatgg aagttataag gaaaatgcaa tcagtaaaga tgaactaaga
    12001 atgattttaa gaaatggtta ccaagaagtt gatccaaagg aaatgacaac tttagttgat
    12061 gactggagta atcaaaaaga agttgaacct aagatttatt atacttttga tggtgaaaag
    12121 attgtatgtg ctgataatac ttctaacgat atgtttatgg aagaattcaa taaagaagat
    12181 tatctattag cttttctttg gatggctaaa ggaatggcaa tggatgaagt gatgattcta
    12241 aagtttcatg actctaaaga tgtagttgca gtaaagtagg tgatcattat cgcagggaca
    12301 agaatcaaat acaacaaggt taaatatcca attaaagtag gtaccaagtc ctatggatgg
    12361 acaatggtag catcctatac acatcataat gtatgggaaa gaataacaag atcaggcgtt
    12421 ccaatcagaa cgtctttttt taaggacgaa gttcctaatg aaaatgcgac gtatacatgg
    12481 gattaattaa ggcataaaaa atgcatatgc aatgtttacg ttataaaagt gaaaaaacat
    12541 cgataaacgt tgttgagttg tacaggaagg taatgtatac gtgatgttca catttaggca
    12601 tacattatcc cttatcctgc taaagaaagg agaagaatac atatggctaa tgatggcaat
    12661 ctaagtatta gatgtactaa tcatactaaa gaaatgttta atgcaattgc aaaaaagaat
    12721 gctcatttat caaagggaga gttattggaa gaaatgatta atagatatca agcagtagag
    12781 actattaata atgatgcgat taatgaagta ctaaaattaa cccagctaaa tcatgaatat
    12841 attgcattca tgatgaagtg taataaatca attccaacga atgttgctac agtaattaaa
    12901 cttattaacg aagaagcaat gtgcgaaaag tacatcagaa tgaagttgag aaaggaaata
    12961 tagttacaag gtaattttta ccttgtggtt ttattatgaa cataagaaaa gcgtattggg
    13021 taagtaaaga ttttgataag aaacttcaag aaatgcaaga gtatatgaag gatgtttgtc
    13081 atttggatta tacagtagct gatttgtttg atgagatgat gtcactgtat gaggctagaa
    13141 taaaatttaa tgcaaatatt cttaacgaat tatttagaga acatgatgaa gctatgttag
    13201 atcaattttt aatcagtaat gctgaaatgc taaattcatt aaaggagtat atagaaggag
    13261 ttacaaggta aatttacctt gttggaatta tgaagataac gaagataaga ttttttgaat
    13321 atggacaaaa gccaccaaaa aattcaagat ttaaagatac aatctcgaat gaaacagttg
    13381 gtggttattt tgattacaca agcagagaag aagctaaaga tgttgataaa gaaattgtga
    13441 aacaagaaga tggttatttt ggatatacag gaag
//
LOCUS       DAOMTA010000005        12337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_19_length_12337_cov_2.677414, whole genome shotgun sequence.
ACCESSION   DAOMTA010000005 DAOMTA010000000
VERSION     DAOMTA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_12337_cov_2.677414"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..4673
                     /gene="gltB"
                     /locus_tag="PLK68_00340"
     CDS             162..4673
                     /gene="gltB"
                     /locus_tag="PLK68_00340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRM88888.1"
                     /translation="MNEKKNLGLYDKSFEHDNCGIGAVVNIKGVKTHITVSNALKIVE
                     QLEHRAGKDAAGETGDGVGILTQVPYSYFKKTIKEFPLPLEGKFGVGMLFMPRDEHLR
                     NRVKKMLETIVIKEGMEFLGWREVPTVPKVLGQKALDAMPSIWQCFIKKPHGINKGIE
                     FDRILYQTRRIFEQSNFDDTYVCSFSSRTIVYKGMFLVGQLRSFFKDLQDDEYKSAIA
                     LVHSRFSTNTMPSWQRAHPNRFIVHNGEINTIKGNANNMLCREEIMTTNLLDTNKVYP
                     VVDINGSDSAMLDNTLEFLVMSGMELPRAVMMCIPEPYDNDKSMSQSKKDFYEYNSTL
                     MEPWDGPASILFSDGEVMGAVLDRNGLRPSRYYITRDDYLILSSEVGALDIPAEDIVI
                     KDRLRPGKMLLVDTKAGKLIADDDLKESYAHKQPYGEWLESNLIKLKDMRIPNARVER
                     YQGEQLTKLQKIYGYSYEDVNTYIKKLALNGSEEVVAMGNDSPLAVLSMQHPPLFNYF
                     KQLFAQVTNPPIDAIREEIITSTALCIGTEGNILADNEKNCRLLKIDHPILSNTDLLK
                     IKNIKKDGFKVAVLSMLYYKNTSLEKALDKLFVEADKAYHEGANILILSDRGVDENHV
                     PIPSLLAVAAMNEHLVNTKKRMSVALILESGEPREVHHYATLIGYGASAVNGYLAQDT
                     IFELVDEGLLDKDYYAAIEDYNDGILHGIVKIASKMGISTIQSYRGSQNFEAIGLAKD
                     FVAKYFPKTVTRIEGKTIKDIENDVDYRHSKVYDPLGLDVDITLDSRGDHKERSGKEE
                     HLYNPATIHKLQLATRNGDYKLFKEYSAMIDEEGKNLNLRGLLQFKKGKSIPLDEVES
                     VDKIVQRFKTGAMSYGSISKEAHETMAIAMNRLKGKSNSGEGGEDPERFILDENGDSR
                     CSAIKQVASGRFGVTSQYLCSAKEIQIKMAQGAKPGEGGQLPAGKVYPWVAKTRHSTP
                     GVGLISPPPHHDIYSIEDLAQLIYDLKNANKDARISVKLVSEAGVGTVAAGVAKAGAG
                     VILISGYDGGTGAAPRNSVYNAGLPWELGLAEAHQTLIMNDLRGRVVLETDGKLMTGR
                     DLAIATLLGAEEYGFATAPLVTMGCVMMRVCNLDTCPVGVATQNPILRKRFTGKPEYV
                     ENFMRFVAQEFREYMAELGFKTIDEMVGRSDLLEVRPGVENVDLSRVINNPYIKAKEI
                     RHNPKNNYDFKLEEVKDTTILLKEFKNALENHQSHEINVDITNIDRTLGTLFGSEITK
                     RYQDTLDDDTFKVNCYGSGGQSFGAFIPNGLTLTLHGDSNDYFGKGLSGGKLVVVPPE
                     NSNFKAAENIIIGNVALYGATSGEAYINGIAGERFAVRNSGAKAVVEGVGDHGLEYMT
                     GGLVVVLGETGRNFAAGMSGGIAYVYDPNNRLYSRINKELVSYSSVTSKYDEEELRAV
                     IQKHYDHTNSEVAKDILDNFGEQVSMFKKVVPHDYKHMIELIKYYEKQGLTNEQAKVE
                     AFNEARKGV"
     gene            4676..6157
                     /locus_tag="PLK68_00345"
     CDS             4676..6157
                     /locus_tag="PLK68_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRM88889.1"
                     /translation="MGKPTGFLEIERQVSKAVEPKKRIQNFNEFHKHLSKDDQACQGA
                     RCMDCGVPFCQSGRELEGAVSGCPLNNLIPEWNDLIYHNNYKQALARLLKTNNFPEFT
                     ARVCPALCEAACTCGLNGEPVTVKENEYGIIEDAYAKGLIKPCPPTHRIDKKIAIVGS
                     GPAGLTAADQLNKRGYQVTVYERDDRIGGLLMYGIPNMKLEKEVIDCRVGIMKAEGVV
                     FKTNCGIEDEKQAKELLDNYDRVILACGSRKPRDIDVPGRQGRGILMAVDYLTAITKS
                     LLNSNLKDKNFAPTKDKHVLVIGGGDTGNDCVGSAIRLGCKSVTQLEMMPELPAIRSD
                     NNPWPEWPRIKKTDYGQEESIAVFNQDPRIYQTTVKEFILDKNGQVKEAVIVSLEPKE
                     NKETKRVEMVPVAGSEQTIPADLVLISAGFIGTEDHVARAFNVTLNPRGNVKSNQDYQ
                     TNIKKLFVAGDMRRGQSLVVWAIKEGREVARAVDFDLMGYSNL"
     gene            complement(6213..6725)
                     /locus_tag="PLK68_00350"
     CDS             complement(6213..6725)
                     /locus_tag="PLK68_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRM88890.1"
                     /translation="MLDKKVVELLNDQVNKEFYSAYLYLDFANYYKDNGLDGFANWYN
                     IQAQEERDHAILFVQYLQNNNAKVTLEAIDKPDKEYTKLNDPLIYGLEHEEYVTSLIH
                     NLYDAAYSLKDFRTMQFLDWFVKEQGEEETNANDLITKFNLFGSDSRSLYLLDSELAA
                     RVYNAPSLVL"
     gene            complement(6797..7327)
                     /locus_tag="PLK68_00355"
     CDS             complement(6797..7327)
                     /locus_tag="PLK68_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88891.1"
                     /translation="MLKKKNNLICMIMIITALISGISTVILDYNLNSNSSAVTSLSTN
                     SNNLDGDNPFGNNDSSTNNSTNPFGNSDSSIFPTTANNQNSKYRISILATCSIIICAL
                     IASLAIIYLIMSKLSQHQVFINNDKKWIYGLSSCLLTILISFICINATKNLDSKTNNP
                     TSIPNSSEQSNNNDIV"
     gene            7500..8327
                     /gene="cdaA"
                     /locus_tag="PLK68_00360"
     CDS             7500..8327
                     /gene="cdaA"
                     /locus_tag="PLK68_00360"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRM88892.1"
                     /translation="MKVGDLMPLISAFTFNISSILNVGRTIIDLILVWYVIYLLISMM
                     KQNMRTMQLFKGVLLILILKMFTSLLRLSAMDYLVDTILTWGVVAIIIVFQPEIRGLL
                     EKIGRTKLELKHDNLSDDEKERLMDELVGAITKLSEDQTGALITFERRQSLIDYINTG
                     TKINADIKAELFTTIFWEGTPLHDGATIIKGDRVVCAAAFYPPTNQELSPLYGARHRA
                     ALGISEITDSLTVVVSEETGTISFATDGKLRKIPRKELRASLVNELDWFNTQEKDGE"
     gene            8329..9744
                     /locus_tag="PLK68_00365"
     CDS             8329..9744
                     /locus_tag="PLK68_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRM88893.1"
                     /translation="MSKKENPKNPIKKDSTTDFFLNFITRQKSEQKDEEKTTTSKVVD
                     DTVKVRDKAFELFYHMINSLNIFLDRMLQSNLSMKVLSFVMAVVLLFTITGGIDNIFS
                     TPNGGDYLYDVKIDTEGLQSDYDVVGLPETVNVALVGPSLDIYSTKISKKYKVVADFS
                     TLGEGEHTIELQGKDFPSDLQVMIVPQTVTVKITQKVTKTFELGYKFSNEDSMDSKYS
                     VSVESMEHHEVEVRGSQDNIDKINSVKAVIDLKGKNNDFEQNAKIYAYDRSGKKVDVE
                     IIPNTVKVDCMVSSYSKEVSIVPQYTGQLASGYGFESIKLKQEKVTIYGKEELLNSIN
                     SVGVVIDLSGLSGDKSYSKLPLTGIENINKLDFNTVDASVRVSPSTKRIITDIPINIV
                     NNNGGYQVNFAEGQDKASVEVDGVAAILDALTINDFNISIDLANLKAGTNTVKVDLKI
                     DKGYLTGKLVSPERITITLRK"
     gene            9760..11109
                     /gene="glmM"
                     /locus_tag="PLK68_00370"
     CDS             9760..11109
                     /gene="glmM"
                     /locus_tag="PLK68_00370"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRM88894.1"
                     /translation="MGKYFGTDGFRGKANVDLTVEHAYKVGRYLGWYYSQNGKAKVVI
                     GKDTRRSSYMFEYALVSGLTASGADVYLLHVTTTPSVSYVVTSEEFDCGIMISASHNP
                     YYDNGIKILDGNGHKMDAGVENLIEQYIDGLIDELPYATKDEIGCALDYSIGRNRYIG
                     YLMSIPTRAFRNYRVGLDCANGASSAIAKSVFDALGAKTYVINSDPDGLNINTNCGST
                     HIEVLQQYVKENALDIGFAYDGDADRCICVDEFGRVVDGDLILYVCGKYLKDHGELAN
                     DTVVTTIMSNLGLYKAFDKEGIKYEKTAVGDKYVNENMVKNGHVLGGEQSGHIIFSKH
                     ATTGDGILTSLKVMEAVIESKRTIAQLIEPVTIYPQLMKNVPVRDKKEAQEDPDVRAV
                     IAEVETDLGENGRVLVRESGTEPVVRVMVEADTDEKCLINVNKIVDKMKEKGFVIKR"
     gene            11184..11837
                     /locus_tag="PLK68_00375"
     CDS             11184..11837
                     /locus_tag="PLK68_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM88895.1"
                     /translation="MQFRINIIDDEKNLNDLVRTYLEKEGYIVYSFYTYDEALMHKDD
                     DVHLWLIDIMLDRASGFELFNEIKASRPKMPIIFMSARDQEFDRIIGLEKGSDDYITK
                     PFNIKEVILRINNLIKRSYDDPSKIKMDGYDIDLEKRRVFNGGEEVILTTKEYDLLVY
                     FITNKGLAISREQVLNKVWDENYYGSDRVVDDTLRRLRKKMPEINVRTIYGFGYRLD"
     gene            11837..>12337
                     /locus_tag="PLK68_00380"
     CDS             11837..>12337
                     /locus_tag="PLK68_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM88896.1"
                     /translation="MLNKFSLQRQLSRFSLQKQLIIIFSLLAMLVILILVPLINKNLN
                     ALIDEEMFKTLDTSQSAYIDFDYSPIAKSSDKQIYHMTYDKNTNYLFPPSNLTRDKVL
                     ALYPVFADKLNEMLKGNKDKIQAKGTLDGDTLYFQITKKDSDSYIISLVYSDYSASLI
                     SSIRQQI"
BASE COUNT         4303 a         1590 c         2476 g         3968 t
ORIGIN      
        1 ttaaccccgt aaaacagcca ttttagtact aaaatggctg ttttacgggg ttaaactagg
       61 aattattttt gtttttttag gatttttcta aaaaattcac atatctttac acatttatcg
      121 aaatagtgta ttatggggct acataaaaag ggggaaccta tatgaacgag aaaaaaaatc
      181 taggattata cgataaatca tttgaacatg acaactgtgg gattggtgca gttgtaaaca
      241 ttaagggagt taaaacgcat attacggtaa gtaatgcatt aaaaattgtt gaacaattag
      301 agcatcgtgc tggtaaggat gctgcaggtg agacgggtga tggtgtcgga attttaacgc
      361 aagtaccata tagttatttt aaaaagacaa ttaaagaatt tccattaccg ttagaaggta
      421 aatttggtgt tggaatgtta tttatgccaa gagatgaaca tttacgtaat cgagttaaaa
      481 aaatgcttga aacaatcgta attaaggaag gaatggaatt tttaggatgg cgtgaagtac
      541 ctactgttcc taaagtatta gggcaaaaag cacttgatgc gatgccttca atttggcaat
      601 gtttcataaa aaaacctcat ggaatcaata aagggatcga gtttgatcga atcctttatc
      661 aaactcgtag aatttttgaa caaagcaatt ttgacgatac ctatgtatgt tctttttcat
      721 cacgaacaat tgtttataaa ggaatgttct tggttggaca attgcgctca ttttttaaag
      781 atcttcaaga tgatgaatat aagagtgcga ttgcgttagt ccattcacgt ttttcaacta
      841 atacaatgcc ttcttggcaa cgggctcatc ctaatcgttt tattgttcat aatggggaaa
      901 ttaacacaat taaaggcaat gctaataata tgttatgtcg cgaagaaatc atgacaacta
      961 atttgctgga tactaataaa gtgtacccag tcgttgatat taacggttca gattcagcaa
     1021 tgctggacaa tacattagaa tttctagtta tgtctggaat ggaattacca agagcagtaa
     1081 tgatgtgtat tccagaacct tatgataatg ataaaagtat gtcacagtca aagaaagatt
     1141 tttatgaata caattcaact ctaatggaac cttgggatgg tccagcttca attttattca
     1201 gtgatggaga agttatggga gcggtacttg atcgaaatgg acttcgccca tctcgttatt
     1261 atattactcg tgatgattat ttgattttat catctgaagt tggagcttta gatattcctg
     1321 ctgaagatat tgttattaaa gatcggttac gcccaggaaa aatgttatta gttgatacaa
     1381 aagctggtaa gttaattgcg gatgatgatt taaaagaaag ttatgcccat aaacagcctt
     1441 atggtgaatg gcttgaaagt aatttgatta aattaaaaga catgcgcatt cctaatgctc
     1501 gagttgaacg ttatcaaggg gaacagctaa ctaagttaca gaaaatttat ggttatagtt
     1561 atgaggatgt aaatacgtat ataaaaaaat tagcattgaa tggtagtgaa gaagtagttg
     1621 caatgggaaa tgattcacca ttagcggtat tatcaatgca gcatccccca ttatttaatt
     1681 attttaaaca attatttgct caagtgacta accctccaat cgatgctatt agggaagaaa
     1741 ttattacttc aacagcttta tgtattggaa cagaaggaaa tattttagct gataatgaga
     1801 agaactgtcg cttgttgaaa atcgatcatc caattttaag taatactgat ttattgaaaa
     1861 tcaagaatat taagaaagat ggttttaaag tagcagtatt atcaatgctt tattataaaa
     1921 atacttcact tgaaaaagcc ttagataaat tatttgtaga agcagataag gcttatcacg
     1981 aaggagctaa tattttaatt ttatcggatc gaggagttga tgaaaatcat gttccaatcc
     2041 cttctttatt agcagttgca gcaatgaatg aacacttagt aaatactaaa aaaagaatgt
     2101 ctgtagcctt gattttagaa tctggagagc cacgtgaagt gcatcattat gcgactttga
     2161 ttggatacgg ggctagtgct gttaatggat atttagcaca agatacaatt tttgaacttg
     2221 ttgatgaagg cttactagat aaagattact atgctgcaat cgaagattat aatgacggta
     2281 ttttgcatgg aattgttaaa attgcatcaa aaatgggaat ttcaacaatt caatcatatc
     2341 ggggttcaca gaacttcgaa gcaatcggtt tagctaaaga ctttgttgct aagtatttcc
     2401 ctaaaacagt aaccagaata gaagggaaga caatcaagga tattgaaaat gatgttgatt
     2461 atcgtcatag taaagtttat gatccattag gattagacgt cgatattaca cttgatagtc
     2521 gtggtgatca taaagaacgt agtggaaaag aagaacatct atacaatcct gcaacaattc
     2581 acaaattaca actagcgaca cgcaatggcg attataaatt atttaaagaa tattcagcaa
     2641 tgattgatga agagggtaaa aatctaaatc ttcgaggatt attacaattc aaaaagggaa
     2701 aatcaattcc gcttgatgaa gttgaaagtg ttgataaaat tgtacaaaga tttaaaacag
     2761 gagcaatgtc atatggctca atttcaaaag aagctcatga aacgatggca attgctatga
     2821 atcgtttgaa aggaaaatct aatagcggtg aaggtggtga ggatccagag cgttttattc
     2881 ttgatgaaaa tggtgatagt agatgctcag caatcaagca ggttgcttca ggaagatttg
     2941 gagtgacttc tcagtattta tgttcagcaa aagaaattca aattaagatg gctcaaggag
     3001 ctaaacctgg tgaaggtggt cagttaccag ctggaaaagt ctatccatgg gtagctaaaa
     3061 caagacatag tacgccggga gtaggattga tttctccacc tccacatcat gatatatatt
     3121 caattgagga tttggcacaa ttaatttatg atttaaaaaa cgctaataag gatgctagaa
     3181 tctcagttaa attagtctca gaagctggtg tcggtactgt cgcagctgga gttgctaaag
     3241 ctggggctgg agtaatctta atttctggat atgatggtgg aactggagca gcacctagaa
     3301 actctgtata taatgcagga ttaccatggg aattagggtt agcagaagct catcaaacac
     3361 ttatcatgaa tgatctgcgt ggccgtgttg tattggagac tgatggtaaa ttaatgacag
     3421 gtcgtgattt agcaatcgca actttattag gggctgagga atacggcttt gcaactgcac
     3481 cgcttgtaac aatgggatgt gtaatgatgc gagtatgtaa tctagatact tgtccagttg
     3541 gagtagcaac acaaaatcca atcttgcgga aaagatttac aggtaagcca gaatatgttg
     3601 aaaactttat gcgatttgta gctcaagaat tccgcgaata tatggcggaa ctagggttta
     3661 aaacaatcga cgaaatggtt gggcgcagtg atttattaga agttcgtcca ggtgttgaaa
     3721 atgtagattt atctagagtg attaataatc catatattaa agctaaagaa atacgtcata
     3781 atcctaaaaa taattatgat tttaaattag aagaagtaaa agatacaaca attttgttaa
     3841 aagagtttaa aaatgcttta gaaaaccacc aatcacatga aatcaatgtt gatataacga
     3901 atatcgatcg aacattagga acattatttg gatcagaaat tactaaacgc tatcaagata
     3961 cattagatga tgatacattc aaagtaaatt gttatggttc tggtggccaa agttttggtg
     4021 cttttattcc taatggttta acattgacat tgcatggtga tagtaatgat tattttggaa
     4081 aaggattatc aggaggaaaa ttagtagttg ttccacctga aaattcaaac tttaaagcag
     4141 ctgaaaatat tattattgga aatgttgctt tgtatggagc tacttcaggt gaggcatata
     4201 ttaatgggat tgctggtgaa cgttttgcag ttagaaattc aggagctaaa gctgttgtag
     4261 aaggtgtcgg agaccatgga ttagaatata tgacaggtgg tttagttgtt gttcttggag
     4321 aaactggaag aaattttgca gccggtatgt ctggtggaat cgcatatgta tacgatccta
     4381 ataatcgttt gtattcacga attaataaag aattagtctc ttattcatct gtgacaagta
     4441 aatatgatga agaagaatta agggccgtta ttcaaaagca ttatgatcac actaattcag
     4501 aagtagctaa agatatttta gataattttg gtgaacaagt atcaatgttt aagaaggttg
     4561 ttcctcacga ttataagcat atgattgaat taataaagta ttatgaaaag caaggtctaa
     4621 caaatgaaca agctaaagtt gaagcattca atgaagctag gaagggggta taaccatggg
     4681 taaaccaaca gggtttttag aaattgagcg tcaagttagt aaggctgttg agccgaaaaa
     4741 gagaattcaa aattttaatg aatttcataa gcatttgagt aaagatgatc aagcctgcca
     4801 aggagcccgc tgtatggatt gtggtgtacc gttttgtcaa tctggtcggg aattagaagg
     4861 cgctgtatct ggctgtccat taaataatct gatcccagaa tggaacgatc ttatttatca
     4921 taataactat aagcaggcat tggcaagatt attaaaaact aataattttc ctgagtttac
     4981 tgctcgggta tgtcctgcct tgtgtgaagc agcatgtact tgtggtttaa atggcgaacc
     5041 tgtaaccgtt aaggaaaatg aatatgggat tattgaagat gcttatgcaa aaggtttgat
     5101 taaaccatgt ccaccaactc atcgtattga taagaaaatt gcaatcgtgg gttctggacc
     5161 agctgggtta actgcagccg atcaattaaa taaacgtgga taccaagtta ctgtctatga
     5221 acgggacgac cgtataggtg gattattaat gtatggaatt cctaatatga aattagaaaa
     5281 agaagttatt gattgtcgtg ttggaattat gaaggctgaa ggtgttgttt ttaaaactaa
     5341 ttgtggaatt gaagatgaaa agcaagctaa agaattatta gataactatg atcgagtaat
     5401 cctagcttgt ggttcacgga agccccgtga tattgatgta ccgggacgtc aaggcagggg
     5461 aattttaatg gcagttgatt acttgacagc aataacaaaa tctttactta atagtaattt
     5521 aaaagataaa aattttgctc caactaaaga taaacatgta ctagttattg gtggtggtga
     5581 taccggtaat gattgtgttg gtagtgcaat tcgtttgggg tgtaaaagtg taacgcagtt
     5641 agaaatgatg ccggaacttc ctgctattag aagtgacaat aatccatggc cagaatggcc
     5701 gagaatcaaa aaaacagatt atggtcaaga agaaagtatt gctgttttca atcaagatcc
     5761 tcgtatttat caaacaacgg taaaagaatt tattctagat aaaaatggtc aggtaaaaga
     5821 agcagtaatt gtttcattag agccaaaaga aaataaagaa actaagcgag tagagatggt
     5881 acctgttgct ggcagtgaac aaactattcc tgcggattta gttttaatat ctgcgggatt
     5941 tattggaact gaagatcatg tagctagagc atttaatgtt acattgaatc ctcgcggaaa
     6001 tgtaaaaagc aatcaggatt atcaaacaaa tataaaaaag ttatttgttg caggtgatat
     6061 gcgtcgagga caatctttag tggtttgggc aattaaagaa ggtcgcgaag ttgctcgagc
     6121 agttgatttt gatttgatgg gttatagtaa tttataaaaa agatcatagc gaataagcag
     6181 ttgtttatcc gttatgacct ttttattttt tgttataaaa ctaatgatgg agcattgtaa
     6241 acacgggcag ctagttcact atcaagtaag tataatgaac gagaatcaga accaaataga
     6301 ttaaatttag taattaaatc attagcatta gtttcttctt caccttgttc tttaacaaac
     6361 caatctaaga attgcattgt acgaaaatct ttaagagaat aggcagcatc atagagatta
     6421 tgaattaaac tagtaacata ttcttcatgt tctaagccat aaattagagg atcatttaat
     6481 ttagtatact ctttatcagg tttatcgatt gcttcaaggg ttactttagc attgttattt
     6541 tgaagatatt gaacaaataa tattgcatgg tctctttctt cttgtgcttg gatattatac
     6601 cagttggcaa acccatctaa tccgttatct ttatagtaat tagcaaagtc taaataaaga
     6661 tatgcggaat aaaattcttt gttaacttga tcatttaata attctacaac ttttttatct
     6721 aacattttaa ttacctcctg tatatagtat agacctattt tatttaattg ttaatagatt
     6781 taacaattat gtaacattag acaatatcat tgttattgct ttgttcacta ctattgggaa
     6841 tactagtagg attattagtt ttactgtcta aatttttggt agcatttata cagataaagg
     6901 agatcaagat tgtaagtaag cagctactta agccgtagat ccatttctta tcattgttaa
     6961 taaaaacctg atgttgagat agtttagaca taattaaata aatgatagct agactagcaa
     7021 ttaatgcaca aataataata ctacaagttg ctaaaattga aattcggtat ttactgtttt
     7081 gattattggc tgttgttgga aagatactgc tatcactgtt tccaaaagga tttgtactat
     7141 tattagttga tgaatcattg tttccaaaag gattatcacc atcgagatta ttactgttcg
     7201 tagatagtga tgtaaccgct gaactatttg aattaaggtt ataatccaag ataactgtac
     7261 taataccgct aatcaaagca gtaatgatca ttatcatgca aattagatta tttttctttt
     7321 ttaacataac tttcacctca cagtaatagt gtaagaaaag atagtatagt taaaatgttt
     7381 acaatatgtg aatatatggc aggtttgtaa atctttatgt cattattttg tcggattaaa
     7441 gaatcttaat tatttgacaa agggttttta atttaaaatg gataatggta taatagttca
     7501 tgaaagtggg tgatttgatg ccgttaattt ctgcatttac atttaatata agttcaatcc
     7561 ttaatgtagg tagaactatt atagatttaa ttcttgtgtg gtatgtaatt tatttattga
     7621 tttctatgat gaagcaaaat atgcgtacaa tgcaattatt taagggagtt ttattgatcc
     7681 taattttaaa aatgtttaca agtttattaa gactaagtgc aatggattat ttagttgata
     7741 caattcttac ttggggtgtg gttgcaatta ttattgtatt tcagccagag attcgaggtt
     7801 tgttggaaaa aatcggacga actaaattag aattaaagca tgataatctt agcgatgatg
     7861 aaaaagaacg attaatggat gagctggttg gagcaattac taaattatct gaggatcaaa
     7921 ctggggcatt aataaccttt gaacgaagac agtcgttaat agattatatt aataccggaa
     7981 caaagattaa tgctgatatt aaagcagagt tatttacgac gattttttgg gaaggaactc
     8041 ctcttcatga tggagcaacg atcattaaag gggatcgggt tgtctgtgcg gccgcctttt
     8101 atccacccac caatcaggaa ttaagtccac tatatggagc tagacatcgt gcagccttag
     8161 gtattagcga gataactgat tcgcttacag ttgtagtatc tgaagaaaca ggaacgattt
     8221 cttttgccac agatggtaaa cttcgaaaaa ttcctcgaaa agagttgcga gcaagtttgg
     8281 ttaatgaact agattggttc aatactcaag aaaaggatgg tgagtaagat gtcaaaaaaa
     8341 gaaaatccta aaaatccaat aaaaaaagat tcaactactg atttcttttt aaacttcatt
     8401 actcgtcaga aatcagaaca gaaagatgaa gaaaaaacaa ctacttctaa agtagttgat
     8461 gatactgtaa aagtgagaga taaagcattt gaattattct atcacatgat caattcttta
     8521 aatatatttt tagatcgtat gttgcaatca aatctttcaa tgaaagtttt atcgtttgtt
     8581 atggctgtcg ttttactctt tacaattacc ggtggaattg ataatatctt ttcaacccct
     8641 aatggcgggg attatctgta tgatgtgaaa attgatactg aagggttaca aagtgactac
     8701 gatgtagtag gattaccgga aacagtaaat gttgctttag tcggaccttc tttagatatt
     8761 tactcaacaa agatatctaa gaaatataaa gtagttgctg atttctctac tcttggggaa
     8821 ggtgaacata caattgaatt gcagggaaaa gattttccta gtgatttgca ggtaatgatc
     8881 gtaccacaga ctgttactgt taaaatcaca caaaaggtta caaagacttt tgagctggga
     8941 tacaaattta gtaatgaaga tagcatggat tctaaatatt cagtatcagt tgaatcaatg
     9001 gaacaccatg aagttgaagt tcgaggttca caagacaata ttgataaaat taattctgtt
     9061 aaagcagtga ttgatttaaa aggaaaaaat aatgattttg aacaaaatgc taagatatac
     9121 gcatatgatc gtagtggtaa aaaggttgat gtggaaatta ttccaaatac ggtcaaggta
     9181 gattgtatgg tttcttctta tagtaaagaa gtttccattg tccctcaata tacagggcaa
     9241 ttagctagtg gatatggttt tgagagtatt aaacttaaac aggaaaaagt aactatatat
     9301 ggtaaagaag aattgttgaa tagtattaat agtgttgggg ttgtaattga tttatcggga
     9361 ttaagtggcg ataaaagtta ttctaaattg ccgcttactg gtatagagaa tattaataag
     9421 ttagatttta atactgttga tgcatctgtg cgagtatcac catcaacaaa acgaataata
     9481 actgatattc caatcaacat tgttaataat aatggtggat atcaagttaa ttttgctgaa
     9541 ggacaggaca aagcttcagt cgaagtagat ggtgtggcag caatcttaga tgcactgacg
     9601 atcaatgatt tcaatatttc aattgatttg gcaaacttga aggcggggac gaatactgtt
     9661 aaagtagatt taaaaataga taaaggatat ttaacgggga aattagtttc tccagaaaga
     9721 attacgatta ccttaagaaa ataggaggaa aaattaacga tgggtaagta ttttggaaca
     9781 gatggattta gagggaaagc aaatgttgac ttaacagttg aacatgctta taaagtaggg
     9841 cgctatcttg gatggtacta ctctcaaaat ggtaaagcaa aagttgtgat tgggaaagac
     9901 acaagaagaa gttcatatat gtttgaatat gcactagttt cagggttaac agctagtggt
     9961 gctgatgttt atttattaca cgtaacaaca acaccttcag tatcatatgt tgtaacaagt
    10021 gaagagtttg attgtggaat catgatttct gcttctcata atccttatta tgataacgga
    10081 atcaaaatct tagatggtaa tggtcataaa atggatgctg gagtagaaaa tttaatcgag
    10141 cagtacattg atggtttaat tgatgaatta ccatatgcaa ccaaagatga aattgggtgt
    10201 gctttagatt attcaatcgg tcgtaatcgt tatattggat atctgatgtc gattcccact
    10261 agggcattta gaaattatcg tgtaggttta gactgtgcca acggtgctag tagtgcgatt
    10321 gctaaaagtg tttttgatgc tctaggagct aagacttatg taattaacag tgatcctgat
    10381 gggttaaata tcaatactaa ttgtggttca acccatattg aagtcttaca acagtacgta
    10441 aaggaaaacg cactagatat tggttttgct tatgatggag atgctgatcg ctgtatttgc
    10501 gttgatgaat ttggacgagt tgtagatggg gatctaattt tatacgtttg tggtaaatat
    10561 ttaaaagatc acggagaact tgctaatgat actgttgtta caacaatcat gtcaaatctt
    10621 ggtttatata aagcatttga taaagaaggt attaaatatg aaaaaaccgc tgttggcgat
    10681 aagtatgtaa atgaaaatat ggttaaaaat ggtcatgttt taggtggtga acaatctgga
    10741 catattattt tctctaaaca tgccactaca ggtgatggga ttttaacatc tttaaaagta
    10801 atggaagctg ttattgaaag taaacgaact atcgcgcaat taattgaacc tgttactatt
    10861 tatcctcaat taatgaaaaa cgttcctgta agagataaaa aagaagcaca ggaagatcct
    10921 gatgtaagag cggttattgc tgaagttgaa actgatttag gtgagaatgg tcgtgtttta
    10981 gttcgtgaaa gtggaactga accggttgtt agagtaatgg ttgaagcaga tactgatgaa
    11041 aaatgtctta ttaatgtaaa taaaattgtt gataagatga aggaaaaagg atttgtaatc
    11101 aaaagataaa ataacataat gtcacacaat attgccatat aatatggcaa tatttcatat
    11161 atattaaaag tgaggtgaaa tacatgcaat ttagaataaa tattattgat gatgaaaaaa
    11221 atttaaatga tttagtaaga acctatttag aaaaagaagg atatattgtt tattctttct
    11281 atacatatga tgaagcttta atgcataagg atgatgatgt tcatttatgg ttgattgata
    11341 ttatgttgga tcgtgctagt ggatttgaat tatttaatga gattaaggct agtcgcccta
    11401 aaatgccaat tatttttatg tctgcacgag atcaggaatt tgatcggatt attggattag
    11461 aaaaaggttc tgatgattat ataaccaaac cttttaatat taaagaagtg attttaagaa
    11521 ttaataattt aattaaacgt tcatatgatg atccatcaaa aattaagatg gatggatatg
    11581 atattgattt agaaaaacgg cgcgtattta atggtggaga agaagtaatt ctaacaacga
    11641 aagaatatga tttattagtt tattttatta ctaataaagg cttagctatc tctcgggaac
    11701 aggttttgaa taaagtatgg gacgaaaact attatggtag cgaccgggta gttgatgata
    11761 ctttaagacg tttaagaaaa aaaatgccag agattaatgt acgcacgatc tatggttttg
    11821 gctatcgttt agactaatgt taaataagtt ttctttgcag cgacagttaa gcagattttc
    11881 tttacaaaag cagttaatta ttatcttttc attattagcg atgcttgtta tattgatttt
    11941 agtaccgttg atcaataaaa atcttaatgc tctaatagat gaagagatgt ttaaaacgct
    12001 agatacttca cagagcgcat atattgattt tgattattca ccaattgcta aatcaagtga
    12061 taaacaaatt tatcatatga cttatgataa aaatactaac tatttatttc caccatctaa
    12121 tctaactcgt gataaagttt tagcgttata tccagtattt gctgataaac ttaatgagat
    12181 gctaaaagga aataaagata agatccaggc taaagggaca cttgatggcg atactcttta
    12241 ttttcaaata actaaaaaag atagtgattc ttatatcatt tcattagtat acagtgatta
    12301 ctctgctagt ttgatttcat caattaggca acaaata
//
LOCUS       DAOMTA010000006        11833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_23_length_11833_cov_2.557480, whole genome shotgun sequence.
ACCESSION   DAOMTA010000006 DAOMTA010000000
VERSION     DAOMTA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_11833_cov_2.557480"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PLK68_00385"
     CDS             <1..1473
                     /locus_tag="PLK68_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM88897.1"
                     /translation="VLITIRTMHRVLQSQRMQLGILKALGFKKRKLLIHYLSHSTFIC
                     LVGSILGYWLGLIIMPALIYPMFEEMYVLPVLTSQPIVLGYLLPIGCTILCMLISFSV
                     CFKYLKMNGATILYSNNLISNSSYSLAWSSNLPFRFQWNIRDIIRNKLRSLMTIFGVL
                     GCTALLFSACGLYDTMTNLTDWNYTKLQNYKYKLNLNDNLNEEQIDYLLNQTKGQTLL
                     ESTAKIVIDEQNLDVSLTVLENNDYIKLAQDLSNFKSLKEGIALSKKTADKLDLKVGD
                     IIKWKGNLDHQEYQSKISLIIRTPNIQGITIMKEEYLQTGHQYYPTAIIGSKNQDCLT
                     NLTNISSIQYQQDLLESFDVLLEAMILIIIILVLGAIILGGIILYNLGVLSYLERYYE
                     FSTLKVLGFKDTQIQKILVQQNIWLSMIGILLGLPVGYLLIEYMISTIQDTMDVINFI
                     ALPSYLYAILGTLVISWVISKIISLRIKHIDMVSSLKAND"
     gene            1559..2839
                     /gene="serS"
                     /locus_tag="PLK68_00390"
     CDS             1559..2839
                     /gene="serS"
                     /locus_tag="PLK68_00390"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRM88898.1"
                     /translation="MIDIAILRENPTLVKENMKKKFQDDKIGLVDEAYKLDKDYREVL
                     TKASELRSKRNKLSKEIGQFMREGNKDAAENNKKQVSAMADELAKLEDLETQYGSELK
                     AIMMKIPNFIDESVPLGKDDSENVEITKYGEPVVPDFEIPYHTEIMENFDGIDMDAAG
                     RVAGQGFYYLMGDIARLHSAILSYARDFMIDRGFTYVIPPYMIRSNVVTGVMSFEEMD
                     SMMYKIEGEDLYLIGTSEHSMIGKFIDNILEEDKLPYTYTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMIVVCKPEDSKMWFEKLWNNTVDLFRSLDIPVRTLECCSGDLADLK
                     SKSIDVEAWSPRQKKYFEVGSCSNLTDAQARRLNIRINGENGKYFAHTLNNTVVAPPR
                     MLIAFLENNLNEDGSVNIPKALQPYMGGKEKITK"
     gene            2856..3683
                     /locus_tag="PLK68_00395"
     CDS             2856..3683
                     /locus_tag="PLK68_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM88899.1"
                     /translation="MKTLYISDLDGTLLNSQGKISDYSIKTINNLINEGMIFTYATAR
                     SLVSASPVTRGLIKNLPLIIYNGTFIVNGETGKLLHKNIFNSKQVAHIKAIMEKNQLK
                     PMVYALVNEKERVTIINESLHEGVKYYLSKRKTDYRINLTLDYLSLYEGEVFYFTIIG
                     DYDNLRPAYESLKDDLDYNITFQQEIYRKEYWLEIMPKSASKASAILKLKELLNCDRI
                     VSFGDAINDLPMFAISDQCYAMANAVTSLKQQATAVIKSNDEDGVAHWLKEHVINTK"
     gene            complement(3685..4290)
                     /locus_tag="PLK68_00400"
     CDS             complement(3685..4290)
                     /locus_tag="PLK68_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM88900.1"
                     /translation="MDSFLKGQQGTIKIGFLKNLDPQLLVTIFSDFINEFPNIKIELT
                     SYANYQLYHALEQRMIDIVLGLSFPKYPDYQKILIKKYPLIVLINKNNPLANQKTVSQ
                     EELPNILFDVRKLYYQDNYPEFEGNLLKIACNQGCAILHAFTKDNCYNDYLKAIPLTP
                     LSEKSVYLIYDQDNYNPLISNFINYLKTTSILNHKNYFLSY"
     assembly_gap    4363..4372
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4373..4488)
                     /locus_tag="PLK68_00405"
     CDS             complement(<4373..4488)
                     /locus_tag="PLK68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM88901.1"
                     /translation="MNFTKAAHNLYISQTAVTKQIQLLETELGTKLFNRDNK"
     gene            4743..5273
                     /locus_tag="PLK68_00410"
                     /pseudo
     CDS             4743..5273
                     /locus_tag="PLK68_00410"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
     gene            5387..5818
                     /locus_tag="PLK68_00415"
     CDS             5387..5818
                     /locus_tag="PLK68_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM88902.1"
                     /translation="MKVLIECEEDNELEVVIRCNKIDDEVRRIISLFEDKHFIIGKLD
                     NRSYQIKIDDIYYLEANEDRAFIYCEDKVYETSLRLYELEDQLDPRLFVRISKSILLN
                     LNKLDNVRALLNGRYEALLINRERLIITRHYVSSFKEKFGM"
     gene            5827..6246
                     /locus_tag="PLK68_00420"
     CDS             5827..6246
                     /locus_tag="PLK68_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HRM88903.1"
                     /translation="MNKLKMFKFYMLTTCVSYTFVILLQTLLFHFNHQNMSNELVVMI
                     FIVCVIVNLLINVTHYLELQEWLTNLLSIIEIAFVVCTANYLCGYTNSIIELDNFIGV
                     TFMSVTVYFFVKAVVFMKNNEDAKKINEKLKRNRHRN"
     gene            6337..7275
                     /locus_tag="PLK68_00425"
     CDS             6337..7275
                     /locus_tag="PLK68_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM88904.1"
                     /translation="MNKVIEIKNLTKCYGDLIALDDFSLDVYEGEILGLLGPNGSGKS
                     TTINTILSLLKYDSGSIKIFNKEMSPTAYDIKSKIGVVFQDVAVFDELSVIENIDYFC
                     GLYIKDKKIRANYVQDAINLVSLNEFTKFKPKQLSGGLLRRLNIACGIAHKPKLIFLD
                     EPTVAVDPQSRNNILEGIKRLNELGATIVYTTHYMEEVEQLCNQIIIIDKGRVIASGT
                     KDELKNMITLGERITIELKSMNQTFIEQLKNSDRVMNIEIENNTVHISYNTKEDNLSK
                     LIDYVRENNVNYTSLFSERPTLNDVFLELTGKELRD"
     gene            7326..8405
                     /locus_tag="PLK68_00430"
     CDS             7326..8405
                     /locus_tag="PLK68_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM88905.1"
                     /translation="MVIFWTLVFPLALATFFNLAFANLTASEEFETVKVALVEEQANS
                     QFKETLQELEKGDDKLIDLQITSLDKAQQLLKDEKITGYYVVSNEIKLVVNNTGIDET
                     ILESIVNEYNSTMSTVENIATLNPSALQSNILNTVNLSKNNFENQNIGGNTDLTVVYF
                     YTLIGMNCLNASFCGLRTTSQIEANLSRQGTRITISPISKLVTVLSGILSAFIIQFAI
                     MMVLMAYLIFGLGVGFGEQTGYIILLIAIGCFTGVGLGNFAGNVLKFKKEDTKISFLS
                     SFSLVLSFFSGMMIIDIKYWMQTNLPILTYINPVSLITDGLYALYYYDNLSRYTTNMI
                     CLFVIGVLLILGSLFFTRRKQYDSI"
     gene            8392..9540
                     /locus_tag="PLK68_00435"
     CDS             8392..9540
                     /locus_tag="PLK68_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM88906.1"
                     /translation="MTVFKKYLKIANTYTLMILAYTAIFLGLAIFTGTYNSTSTDYKS
                     MDVKVAIINRDKNTELINGFKDYIKDHGELISLEDDNESLRDALFYRTVDYIMIIPDN
                     YTTDFINGKDVTIETMELPDAYSSIYSKNLLNKYLNTANLYLKAGISDTELSKLIKED
                     LNKKVEVGMLDKQNEVDFTMPATYYNFSNYMLITITMVIVTMIMVSFNEEKIKRRNLV
                     SPVSYKSMNRQLMLGNYTVGLAIWLLYVGFSFILYKDAMLTMNGLFLVLNSLVLMIFI
                     QAFSFMIAKFTSNREILSGVGNLFGMGSSFICGAFVPQSMLSPFVLSLAKFLPSYWFI
                     KANNEIIKLTDFSFGSIKPILIDMLIIFGFTLLVYLATQIVTKIRLKK"
     gene            9575..10078
                     /locus_tag="PLK68_00440"
     CDS             9575..10078
                     /locus_tag="PLK68_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM88907.1"
                     /translation="MEIEYLEAVPSDALKIINYLNVVAGQSDNLTFGLNECILNEVQE
                     MQLIKEIHEDPNSVMIVAKDGDEIVGIATLSGNQKTRLSHRAQMGVSVLKSYWHQGIG
                     SKLVAIIIGYAAEASIEIIELEVVTSNENAIALYQKYGFEVIGTYENFMKIDDHYVDA
                     YLMNLYL"
     gene            10816..10901
                     /gene="ffs"
                     /locus_tag="PLK68_00445"
     ncRNA           10816..10901
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLK68_00445"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            10919..11377
                     /locus_tag="PLK68_00450"
     CDS             10919..11377
                     /locus_tag="PLK68_00450"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRM88908.1"
                     /translation="MDQDLEFMEIAYQEALKCLDMDEVPVGAVIVKDGKIIACGRNLR
                     ETSKRATAHAEIIAIEEACRTLNSWYLDECTLYVTLEPCVMCSGAIINSRIQRVVFGA
                     FESRWLALTTIYQSDIPVNHQPVIVSGVLGDKCSKVIKDYFKNKRKRDKS"
     gene            11439..>11833
                     /locus_tag="PLK68_00455"
     CDS             11439..>11833
                     /locus_tag="PLK68_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM88909.1"
                     /translation="MSYKALYRSYRPQTFGEVAGQEHIVTTLKNAIKENRISHAYLFA
                     GPRGTGKTTVAKLLAKALNCTGENPPCDQCPNCKAITVGEHPDVIEIDAASNNGVDEV
                     RDLIDKVKYAPINGKYKVYIIDEVHMMSTG"
BASE COUNT         4286 a         1299 c         2039 g         4199 t
ORIGIN      
        1 gttttaatta cgattagaac aatgcatcgg gttttgcaaa gtcaacgaat gcagttagga
       61 attttaaaag cattaggttt taagaaaaga aagctactga tccattattt atcacattca
      121 acatttattt gtttagttgg ttcaatatta ggttactggc ttggtttaat aataatgcca
      181 gctttgattt atccaatgtt tgaagaaatg tatgttttac cggttttaac tagccaacca
      241 atagttttag gatatttatt accaattggc tgtactatat tatgcatgtt aattagtttt
      301 tctgtctgtt tcaaatattt aaaaatgaat ggtgcaacaa ttctttatag caataattta
      361 attagtaatt caagttattc actagcatgg agcagtaatc taccatttcg atttcaatgg
      421 aatattcgtg atattattcg aaataaattg cgtagtctta tgactatttt tggagttttg
      481 ggctgcactg cattattatt tagtgcttgt ggcctatatg atacaatgac taatttgacc
      541 gattggaatt atactaaatt acaaaattat aaatataaac ttaatttaaa tgataatctt
      601 aatgaagagc aaattgatta tcttttaaac cagactaaag gacagacatt attagagagt
      661 acagctaaaa tagtaattga tgaacaaaat ttagatgttt cgttaacagt cttggaaaat
      721 aatgattata tcaaacttgc tcaagattta agtaatttta aatctttaaa agaaggaatt
      781 gcattatcaa aaaaaacagc cgataagctt gatttaaagg taggagatat aattaagtgg
      841 aaaggaaatt tagatcatca agaatatcaa tctaaaatat ctttaattat tagaaccccg
      901 aatatccaag gaattacgat tatgaaagaa gaatatttgc aaactggtca tcaatactat
      961 cccactgcaa tcattgggag taaaaatcaa gattgcttga ccaatctaac aaatatttct
     1021 tcaatccagt atcagcagga tttattagaa agttttgatg ttttattaga ggcaatgatc
     1081 ctaattatta tcattctagt tttaggtgca attattttag gtggaattat tttatataat
     1141 ttaggagtac tgtcttactt agaacgttat tatgaatttt ctacattaaa agtattagga
     1201 tttaaagata ctcagattca aaaaatcttg gttcaacaaa acatatggtt atcaatgata
     1261 ggaattttat taggtttgcc tgtaggatat ttattgattg aatacatgat atcaacgatt
     1321 caagatacaa tggatgtaat taatttcatt gctttacctt cttacttata tgcaatttta
     1381 ggcactttag ttatttcctg ggtaataagt aaaattattt cattaaggat aaaacatatc
     1441 gacatggttt ctagtctaaa agcaaatgat taaaagagaa acatcttgat aataattgaa
     1501 taaggtgcat aattattgta taattttaaa gaatatatat aagcaaggga gaattaaaat
     1561 gattgatatt gcgatattaa gagaaaatcc aaccttagtt aaagaaaaca tgaaaaagaa
     1621 atttcaagat gacaagattg gattagtaga tgaggcttac aaattagata aagattatcg
     1681 agaagtttta actaaagcta gtgaattgag aagtaaacgt aataaattat ctaaagaaat
     1741 tggtcaattt atgcgtgaag gaaataaaga tgcggctgaa aataataaga aacaagtaag
     1801 tgcaatggca gatgaattag ctaaattaga agacttagaa actcaatatg gtagtgagtt
     1861 aaaagcaatt atgatgaaaa ttcctaattt tatcgatgaa tccgttccat taggaaaaga
     1921 tgatagtgag aatgttgaaa ttactaaata tggggaacca gtcgtaccag attttgaaat
     1981 accttatcat actgaaataa tggaaaattt tgatggtatt gatatggatg cagctggaag
     2041 agttgctgga caaggttttt attatttaat gggtgatatt gctcgtttac attctgcaat
     2101 tttatcatat gctcgtgatt ttatgattga tcgtggcttt acatatgtta ttccgccata
     2161 tatgattcgt agtaatgtag ttactggtgt tatgagtttt gaagaaatgg atagcatgat
     2221 gtataaaatt gaaggggaag atttatattt gattggtact tctgaacact caatgattgg
     2281 taaatttatt gataacattt tagaagaaga taaattacca tatacgtata cttcatattc
     2341 accatgtttt agaaaagaaa aaggggctca tggaatagag gagcgtggag tatatcgaat
     2401 tcaccaattt gaaaaacaag aaatgattgt tgtatgtaaa cctgaagaca gtaagatgtg
     2461 gtttgaaaaa ctatggaata atactgttga tttatttaga tcattagata taccagtaag
     2521 aactttagaa tgttgttctg gagatctagc tgatttgaaa tctaaaagta ttgatgttga
     2581 agcttggtca ccaagacaaa agaaatattt tgaagttggt tcatgttcaa atctaactga
     2641 cgctcaagca agaagattaa atattagaat taatggtgaa aatggaaaat attttgctca
     2701 cactttaaat aatacagtag ttgcaccacc aagaatgttg attgctttcc ttgaaaataa
     2761 cttaaatgaa gatggcagtg taaatattcc taaagcacta caaccatata tgggaggtaa
     2821 ggaaaagata actaaatagt tatcttttta tctatatgaa aactctttat ataagtgatt
     2881 tagatggaac gctattgaat agccaaggaa aaattagtga ttacagtatt aaaacaatta
     2941 ataacttaat taatgaggga atgattttta catatgctac tgctcgttct ctagtttccg
     3001 cttcaccagt aacaagagga ttaatcaaaa atcttccttt gataatctat aatggtacat
     3061 ttattgtcaa tggcgaaact ggaaaactgc ttcataagaa tatttttaat tcaaaacaag
     3121 tagcgcatat aaaagcaata atggaaaaaa accaattaaa acctatggta tatgcactag
     3181 taaatgaaaa agaacgagta acgataataa atgaaagcct tcatgaaggg gtaaaatatt
     3241 atcttagtaa gcgtaaaacc gattatcgaa ttaatttaac attagattac ttatctttgt
     3301 atgagggtga agttttctat ttcaccataa ttggagatta tgataatctt cgaccagctt
     3361 atgaaagttt aaaggatgat ttagattata atattacttt tcaacaagaa atatatcgta
     3421 aagaatactg gctagaaatt atgcctaaaa gtgcctctaa agcaagtgct attttaaagt
     3481 tgaaagaact tttaaactgt gacaggatag ttagttttgg tgatgcaatc aacgatttgc
     3541 caatgtttgc aatatctgat caatgttatg caatggctaa tgcagtaacc tctttaaaac
     3601 aacaagctac agcagttatc aaaagtaatg atgaagatgg tgttgctcac tggcttaagg
     3661 agcatgttat aaacactaaa taaatcagta agataaaaaa tagtttttat gatttaatat
     3721 cgaagtagtt tttaaataat taataaaatt actgatcagt ggattataat tatcttgatc
     3781 atagattaga taaacacttt tttcacttaa tggtgttaag ggaatagctt taagataatc
     3841 gttatagcaa ttatcttttg taaaggcatg caaaattgcg caaccttgat tacaggcaat
     3901 ctttaataaa ttcccttcaa attcaggata attatcttga tagtataatt ttctaacatc
     3961 aaataaaata tttggaagct cctcttggct aacagttttt tgattagcta aggggttatt
     4021 tttatttatt aatactatta aaggatattt ttttattaat attttttgat agtcaggata
     4081 tttaggaaat gataatccta ggactatatc tatcatcctt tgttcaagcg catgatataa
     4141 ttgataattt gcatagcttg ttaattctat ttttatatta ggaaattcgt ttataaaatc
     4201 agaaaatata gtaaccaata attgaggatc taaattttta agaaaaccaa ttttaattgt
     4261 accttgttgt ccttttaaga aagaatcaat atttaatttt gaatgattaa attgattaat
     4321 tattttttag cttcaattaa aaaaaatcta ccagcttcgg tcnnnnnnnn nntgtttatt
     4381 atcgcgatta aataatttag ttcctaattc agtttctaat aattgtatct gttttgtaac
     4441 tgcagtttgc gatatatata gattatgtgc tgctttagta aaattcattg tcttacaaat
     4501 tgctaaaaaa taatttattt gattaagatt catttcatat tcccctttaa taacttttaa
     4561 ttattatata atatcttttt aatattagaa agttattgat taatatatta ctatcttggt
     4621 gatggaggaa tataaatgaa taaaaatgtt aatcgttgaa tcatctacat actatcaatc
     4681 aatattttgg ctttgggaat aattttgaat acccgcactg atttgggggt gctgcgttca
     4741 ctagtctttt ttatgctttt agtaaggtca acaatatttc attaggaact gcttcaatta
     4801 ttttatattt gattttaatt attgttcaga tttgtttagt tagaaaatta actattacaa
     4861 tttttttaga aattccattt tcattaatct ttggatatat tatcgatttt tatgattctt
     4921 taattaaaat taggtgttca aatttattat ctgcttatct actattactg attgcaatta
     4981 tttttgtttc acttggagta tacttttcag ttagctgtaa tcttattgca acacccgttg
     5041 aatcaacggt taagactata tcgcaagtat ataatcttaa attttcgtta gtaaaaaatg
     5101 tatttgatat tactatgatt attatgatgc tcttgctttg tttagtctta cagataccag
     5161 tttatggaat tggtatggga acagtgttat ctgctctgct ggttggtaga tttatttcgg
     5221 gctatcaata tctttttgat gaaaaaattc agatatatca attatctaga taaaagaatg
     5281 ttttagcatt ctttttttgc atcttagcta atctatgtgc atcttaccaa aattagatag
     5341 gtaaaaaaga tattatatga taaggtgtta ataaaggagg gattcgatga aggtgctcat
     5401 tgaatgtgag gaagataatg aattagaagt agtaattcgt tgtaataaaa ttgatgatga
     5461 agtgaggcga attatttctt tatttgaaga taaacatttt attattggaa aattagacaa
     5521 tcgtagttat caaattaaaa tagatgatat ttattattta gaagcaaatg aagatcgtgc
     5581 ttttatttat tgtgaagata aagtctatga aacaagccta agattatatg aattagaaga
     5641 tcaattagat ccccgattgt ttgtaagaat cagtaaatca atattgctaa atttaaacaa
     5701 attagataat gtaagagctc tattaaatgg gcgttatgaa gcgctattaa ttaatcgtga
     5761 aaggttaatt attacacgtc attatgtaag cagttttaag gaaaaatttg gaatgtaggt
     5821 gataagatga ataaattaaa aatgtttaaa ttttatatgt tgacgacttg tgtatcatat
     5881 acgtttgtaa ttttattgca aacattatta tttcatttca atcatcaaaa tatgtctaat
     5941 gaattagttg tgatgatttt tatcgtttgt gtaatcgtaa acttattgat aaacgtaact
     6001 cattacttag aattacagga gtggttaacc aatttacttt caattatcga aatagctttt
     6061 gtagtctgta ctgctaatta tttatgtgga tatacaaaca gtattattga attagataat
     6121 tttataggtg ttacatttat gtcagtaact gtttattttt ttgtaaaagc agtcgttttt
     6181 atgaaaaata atgaagatgc caaaaaaata aatgaaaaat taaaaagaaa ccgccatcgc
     6241 aactaatagt tgcttaaaag aaaccatgct gcaatctaaa attggtggtt tgaaataaaa
     6301 gttttacgta tagtgatgat gtaaggagga gtaaagatga ataaagttat tgagataaag
     6361 aatttgacta aatgttatgg tgatttaatt gctttagatg actttagttt agatgtttat
     6421 gaaggggaaa tattagggtt attaggacct aatggcagtg gtaaatcgac aactatcaat
     6481 acgatcttat cattattaaa gtatgattct ggttcaatca agatttttaa taaagagatg
     6541 agtcctactg cttatgatat aaaaagtaaa attggtgttg tttttcaaga tgtagctgta
     6601 tttgatgaat taagcgttat tgaaaatatt gattattttt gtggtttata tattaaagat
     6661 aaaaaaattc gtgccaacta tgttcaagat gcaattaatc ttgttagtct aaacgaattt
     6721 actaaattta aaccaaagca attaagtggg ggattattac gaagattaaa tattgcatgt
     6781 ggaatagcgc ataagccaaa attaattttt ttagatgagc caacagttgc agttgatcca
     6841 caaagtagaa ataatatttt agagggaatt aaacgattaa atgaattagg agcaacaata
     6901 gtttatacaa cccattacat ggaagaagtt gagcagctat gtaatcaaat tattattatt
     6961 gataagggga gagtaattgc atctggaact aaagatgaat taaaaaatat gattactctt
     7021 ggggagagaa ttacgattga attaaaatca atgaatcaaa catttattga gcagcttaaa
     7081 aatagcgatc gtgtaatgaa tattgagatt gaaaataata ctgttcatat atcttataat
     7141 acgaaagaag ataatttaag taagttaatt gattatgttc gagaaaacaa tgttaattat
     7201 actagtttat ttagtgaacg tccgacatta aatgatgtat tcttagaatt aacaggaaaa
     7261 gagttgagag attaatgttc tatcatttct ttaaatatca atttaaaaaa ttgcttagga
     7321 acaagatggt aatattttgg acgttagtat ttccacttgc tctagcgact ttctttaacc
     7381 tagcatttgc taatttaaca gcaagtgaag aatttgaaac agttaaagtt gcacttgttg
     7441 aagagcaggc taattcacaa tttaaagaaa cattgcagga attggaaaaa ggtgatgata
     7501 agttaattga tttacaaatc acaagtttag ataaagccca gcagttatta aaagatgaaa
     7561 aaattacggg ttattatgtt gttagtaatg aaataaagtt agtggtaaat aatacaggaa
     7621 ttgatgaaac aattttggaa agtattgtta atgagtataa cagtacaatg agcactgttg
     7681 aaaatatagc aacattaaat ccaagtgcat tacaatctaa tattttaaat acagtcaatt
     7741 tatctaaaaa taattttgaa aatcagaata ttggtggaaa cacggattta acagtagtat
     7801 atttttatac tttaattggg atgaactgtt taaatgctag tttctgtgga cttagaacaa
     7861 ctagtcaaat tgaagcaaat ctatctagac aaggaactcg aattactata tcaccgattt
     7921 caaaactcgt tacggtttta tcgggaatct tatctgcctt tattattcaa tttgcaataa
     7981 tgatggtatt aatggcttac ctgatctttg gattaggtgt tggttttggt gaacagacgg
     8041 gttatattat tcttttgatt gcaatcggat gttttactgg agtaggatta ggaaattttg
     8101 ctggaaatgt tcttaagttt aaaaaagaag atactaaaat atcattttta tcaagctttt
     8161 cactagtttt atcattcttt tcaggaatga tgatcattga tattaaatac tggatgcaaa
     8221 caaatcttcc tattttaact tatattaatc cagtaagttt aattactgat gggttatatg
     8281 ctttatacta ttatgataat ttaagtcgtt atactactaa tatgatctgc ttatttgtaa
     8341 tcggagttct tctaatctta ggatcactat tctttacaag gaggaagcaa tatgacagta
     8401 tttaaaaaat atctaaaaat tgctaatact tatacactta tgattttggc atatactgct
     8461 atcttccttg ggttagcaat ttttacggga acttataatt ctacttctac tgactataaa
     8521 agtatggatg ttaaagtcgc tattataaat cgggataaaa atacggaatt aattaatggt
     8581 tttaaagatt atattaaaga tcatggcgaa ttaattagtt tagaagatga taatgaatct
     8641 ttacgtgatg cgttatttta tcgtacagtt gattatatta tgatcattcc agataattac
     8701 actactgatt ttattaatgg taaagatgta actattgaaa caatggaatt gccagatgca
     8761 tatagtagta tttatagtaa gaatcttttg aataaatatt taaatacagc taatttatat
     8821 ttaaaagcag gaattagtga tactgagtta agtaagttaa ttaaagaaga tttaaataaa
     8881 aaagttgaag tggggatgtt agataaacaa aatgaggttg attttacaat gccggcaact
     8941 tattataatt tctctaatta tatgttaata acaataacaa tggtaattgt gacaatgatc
     9001 atggtttctt tcaatgaaga aaagatcaaa cggcgtaatt tagtttcacc agttagttat
     9061 aaaagtatga atcgtcagct aatgttaggt aattatacag ttggattagc tatctggcta
     9121 ttatatgtgg gattcagttt tattctttat aaagatgcga tgttgacaat gaatggatta
     9181 tttttagtac ttaattcact agttctaatg atatttattc aagcgtttag ttttatgatt
     9241 gctaaattta caagtaatcg agaaatttta agtggtgtag gaaatctctt tggtatgggt
     9301 tcgagtttta tttgtggagc atttgtacca caaagtatgt taagtccttt tgttttaagt
     9361 ttagcaaaat ttttaccatc atattggttt attaaagcaa ataatgaaat tattaaatta
     9421 actgatttta gttttggatc aattaaacct attttaattg atatgctaat tatcttcggc
     9481 tttactttat tggtatatct tgctacacaa attgtcacaa agatacgttt aaagaagtaa
     9541 atatgctata atcatcttac aagaaggtga ttgaatggaa attgaatatt tagaagcagt
     9601 acctagtgat gcattgaaaa taattaatta tttgaatgtg gtagctggcc aaagtgataa
     9661 cttaactttt ggtttgaatg aatgtatttt gaatgaagta caggagatgc agttaatcaa
     9721 ggaaattcac gaagatccta attccgtaat gatagttgcc aaagatggcg atgaaattgt
     9781 tggaattgcc acactaagtg gtaatcaaaa gacacgatta agccatcgag cgcaaatggg
     9841 tgtaagtgtt ttaaaaagtt attggcatca aggaataggt agtaagttag tcgctattat
     9901 aattggatat gctgctgaag caagtattga aattatcgaa ttagaagttg ttacgagtaa
     9961 tgaaaatgct attgctttat atcaaaagta tggttttgag gtaatcggaa catatgaaaa
    10021 ttttatgaaa atcgatgatc attatgtgga tgcttatctt atgaatttat atttataaga
    10081 aacaaatagg gcaataactc tatttgtttt ttatttactc taattgtcat agcgagagta
    10141 aacatgtaag ttttgtaatt gttgagtttg attttaatga aacagataaa attgaaaaaa
    10201 ttatttgaat tcattaatat tacttgttga agacgatagt aaaataattg gtgatcattc
    10261 acttgaagga atgaaagtat tatcagtgtt aaaggattat tggtattaag taattaacaa
    10321 tgataaagcg gtacgattat ctaataaatt agactttgaa atcatcagaa catatgacaa
    10381 atatatgtgt atttaaaata ttatgatact tatttaatga atttatattt aaaataaaaa
    10441 caagtagagg gatagctact tgtttctatc attcagatag agggaaaact atctgttttt
    10501 atattgatat cagggaaagg atatcatgaa gagtagtcaa ttagagaaat gattgaaaaa
    10561 actatttaaa actatcctga ctacagttgt tagtatagaa aataactatc gttagaatta
    10621 gttataattg taccaaaata aattttattt atggttaatt atggtttaga aaaaatagtg
    10681 attaaaatgt agtgagaaat ttataattta tattagtgat caatattttt aaggtatgtt
    10741 gtaaattgaa aataatttta tagattcaat attaaaaatg attgaattta ttatatagat
    10801 acggtataat catacttgcc attacaatag gcggcttcga accatgtcag gaccggaagg
    10861 tagcagcatt aagaatcccc gtctatgtgt agtggttttt tgtataaagg agaaagtcat
    10921 ggatcaagat cttgaattta tggaaattgc atatcaagag gctttaaaat gtttagatat
    10981 ggatgaagtg ccagtaggag cagtgatagt taaagatgga aagattattg catgtggacg
    11041 caatcttcgt gaaaccagta aaagagcaac ggcacatgct gagataatag caattgaaga
    11101 agcctgtcga acattaaact catggtatct tgatgagtgt acattatatg tgactcttga
    11161 accttgtgta atgtgctctg gtgctataat taatagtcga attcagcgag ttgtatttgg
    11221 tgcttttgaa tcacgctggt tagccctaac tacgatttat caatcggata ttcctgttaa
    11281 tcatcagccc gtgattgtct ccggggtatt aggagataaa tgctcaaaag tgataaagga
    11341 ttattttaaa aacaaacgta agcgggataa aagttaattc ttttatcctt tttattagat
    11401 aatatagtat aataaataag aatgaggagt gattagtgat gtcatataaa gctttatatc
    11461 ggtcatatcg gccccaaaca tttggagaag ttgccggtca agaacatatt gtaacaactt
    11521 tgaaaaatgc tattaaagaa aatagaattt cacatgctta tttatttgcc ggaccacgtg
    11581 gaacagggaa aacaacagta gctaaacttt tagccaaagc attaaattgt actggtgaaa
    11641 atcctccatg tgatcaatgt cctaattgta aggctattac tgttggggaa catcctgatg
    11701 ttattgaaat tgatgctgcg agcaataatg gggttgatga agttcgagac ttgattgata
    11761 aagtaaaata tgcaccaatt aatggtaaat acaaagtata tattattgat gaggttcata
    11821 tgatgtcaac tgg
//
LOCUS       DAOMTA010000007        11810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_24_length_11810_cov_2.470693, whole genome shotgun sequence.
ACCESSION   DAOMTA010000007 DAOMTA010000000
VERSION     DAOMTA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24_length_11810_cov_2.470693"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="queC"
                     /locus_tag="PLK68_00460"
     CDS             complement(<1..662)
                     /gene="queC"
                     /locus_tag="PLK68_00460"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRM88910.1"
                     /translation="MKALVLFSGGVDSTTALALAIQEHGKDNVVALSISYGQKHTKEI
                     EVANKIAQFYQVEHLYLDLAKIFTYSNCSLLQHSDNEIPHESYNEQLNKTDGNPVSTY
                     VPFRNGLFLSSAASIALSKGCNIIYYGAHSDDAAGSAYPDCSKVFNNAMNQAIYEGSG
                     HQLEIKAPFVEMTKADIVKIGLSLGVPYQLTWSCYEGNDTPCGTCGTCIDRQNAFLKN
                     GI"
     gene            complement(674..>1108)
                     /gene="folE"
                     /locus_tag="PLK68_00465"
     CDS             complement(674..>1108)
                     /gene="folE"
                     /locus_tag="PLK68_00465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRM88911.1"
                     /translation="MNYSNLEIATMFDKTFVDDLDFDNQEVVVIKDIDIFSYCEHHLA
                     LMYDMKVTVAYIPCGKVIGLSKIARIADMAARRLQLQERIGTDIAEIISLVTNSKDIA
                     VIIEGKHSCMSSRGIKKVNSTTVTSTLTGRFKTDSKLQIYLH"
     assembly_gap    1109..1118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1253..1915)
                     /gene="queE"
                     /locus_tag="PLK68_00470"
     CDS             complement(1253..1915)
                     /gene="queE"
                     /locus_tag="PLK68_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRM88912.1"
                     /translation="MNNYKVVEKFISINGEGSRAGQLAAFIRFHYCNLNCSYCDTRYA
                     NDSNSNYELLSAQNILDYLKANKVVNVTLTGGEPLLQQNIDYLIDLLLKNGFSVEIET
                     NGSIDIKPFIKETRPIFTLDYKVPSSTMENEMCLNNYQYLTKNDVVKFVVSNLSDLNK
                     AKEIIDTYDLVNRTKVYFSPVFGKIEPRMIVDYMVKHHLNGINMQLQMHKFIWDINQR
                     GV"
     gene            complement(1918..2334)
                     /gene="queD"
                     /locus_tag="PLK68_00475"
     CDS             complement(1918..2334)
                     /gene="queD"
                     /locus_tag="PLK68_00475"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRM88913.1"
                     /translation="MYFLKTEQSFDSAHFLAGYHGKCANIHGHRWKVIATIKSEKLLE
                     DPQNKGMVTDFGDLKKDLKIIADSFDHALIIETGSLSEKLYQALIDENFKIINLPFRP
                     TAENLAKYIYEALSKDYLVDCLEVYETPNNCASYRG"
     gene            2609..4261
                     /locus_tag="PLK68_00480"
     CDS             2609..4261
                     /locus_tag="PLK68_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM88914.1"
                     /translation="MELTDFFAMFGGLALFLYGMTMMSNGLELAAGNKMKTILEKLTT
                     NRFLGVGVGAVITAVIQSSSATTVMTVGFVNAGLMKLENAVWVIMGANIGTTITGQLI
                     AIDITALAPVIAFVGVALIAFFKSKKLDAIGGIIAGLGILFMGMEMMSSAMVPLRSSP
                     EFVNLVTTFENPLIGILVGAGFTAIIQSSSASVGILQALAMSGVITLPSAIYVLFGQN
                     IGTCITAVLASIGTGRNAKRTTIIHLSFNIIGTIVFVFISMLTPFASFMQSLTPTNIP
                     AQIANVHTVFNVVTTLLLLPFGAQLVKLSYLVLPEKEGFEDKLSVKFLDNSIFTNDYH
                     IGTSAIANTQLFNETQNMLNIVQKNVQRAFDLIIKYDEKTHEKLLKDEQYIDYLNKEI
                     IQFTTNAISNEFPIDDSKSIGLFLKTAGDLERVGDHAVNIAERAEKLYSEDEHFSDEA
                     MREIKIMNDLTRNILEELNVLNRDELHNIVEKVDVIEDSIDITTHEFSLNQLRRLRDK
                     KCTPEHSALYTETLIDFERIGDHGLNIAVAFDEIKDDLTEMA"
     gene            complement(4324..4611)
                     /locus_tag="PLK68_00485"
     CDS             complement(4324..4611)
                     /locus_tag="PLK68_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM88915.1"
                     /translation="MIQNRFNQNSLYILDEPEASLSPQRQLTLLITIYELAKQGSQFI
                     IASHSPILLGIPGAKILNFDSKEIIPIDYQDTESYQITELFINNRADLLNK"
     gene            complement(4696..4965)
                     /locus_tag="PLK68_00490"
     CDS             complement(4696..4965)
                     /locus_tag="PLK68_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM88916.1"
                     /translation="MLQLNTLIFDNPITFFVGENGTGKSTLLEAIAIKYGFNPEGGTL
                     NFNFHTNNTHSSLHHAIILDRGYRRPDEGFFLRAESFYNVATQLE"
     gene            5156..5923
                     /locus_tag="PLK68_00495"
     CDS             5156..5923
                     /locus_tag="PLK68_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM88917.1"
                     /translation="MKIDRTYAIYFSPTYTSKKSAVSIARGLEGELSEIDLTLEETIP
                     EMTFSRHDIVVFGFPVYGGRILHEALERLKSFRGDHTSCVITVTYGNRHYDDALLELF
                     NTVKEQGFIPIAGAALVGEHTYGQIQVGRPNRDDLYRDELFGSLVRLKIKDDNFSFVS
                     VPGKYPYKEGGTGGKFRPETNEQCSGCGVCVNMCPTNAIDIEDCKTINNDCIACFRCI
                     RICPVHAKSMNNNQEYQTFAESFSKKLATPRENEYFI"
     gene            6075..7046
                     /locus_tag="PLK68_00500"
     CDS             6075..7046
                     /locus_tag="PLK68_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM88918.1"
                     /translation="MKKLNWGIIGSGVIANEMAQALLDVNGEIYAVGHRDMNKAIDFA
                     MKYKIKNAYGSVEELLNDPDVDVVYIATPHNSHYEIMKQAVAVKKHVLCEKAITVNDR
                     QLEEIVALAKKNNVVVQEAMTIFHMPLYKKLKEMVAAGVIGNVKMIQVNFGSCKEYDV
                     NNRFFSKELAGGALLDIGVYATSFARYFLSSKPNVVITTADYFETGVDEQSGIIMKNQ
                     DGQMVVMALTMRAKQPKRGVISGELGYIEINNYPRSNQATITYTKDGHQEVIECGDDK
                     QGLQYEVIDMQDYIINNLGEYELQLTRDVSSLLSQIRTQWGMIYPFE"
     gene            complement(7743..8381)
                     /locus_tag="PLK68_00505"
     CDS             complement(7743..8381)
                     /locus_tag="PLK68_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88919.1"
                     /translation="MKTLSTKASKQKILSLLLLAGLWSIMLIIVLLAAKSTWAKNLNS
                     PYWAPAIITIGIIFFITYFIFTVRALFYRQHGYDNQYIYYYKSTSYFNILKLIINTLF
                     NKEMYQDKIAFKDIKKCEIGWYNQTFRIHSFGSEVAHPIYFKINEQMVIKTDLTSNNE
                     HIIELANLLKSHVKDFKDPYHLVLAMNDRSITVYEYIERIIHNKIIKPNLYK"
     gene            complement(8378..9085)
                     /locus_tag="PLK68_00510"
     CDS             complement(8378..9085)
                     /locus_tag="PLK68_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM88920.1"
                     /translation="MKKIGLMVVDNSVIKQLKELIIKNIDSEYRIIEIHDKEMLFTKK
                     MDFCIIEDSFDNQGINYAKTLIENNNDMLVLIISKDGSTIFDAVGINISSYLRYQDIK
                     AKFVPAIQDIIDYLDKRKLLLFKSDQGIVNAQTNDIVYFEYLNRMTYLNTLDKTYRIF
                     IQNFEYLQKMLPKTFFKISRSAIINIFRLITLNSENVIMKNYENKDIAISRRRYQDLL
                     VVLKKHAISFTERKLKK"
     gene            9287..9874
                     /locus_tag="PLK68_00515"
     CDS             9287..9874
                     /locus_tag="PLK68_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM88921.1"
                     /translation="MRKNIITISREFGSGGRTIGKEVAKRLNIPFYDKELIEKVARES
                     GLNVNYIEEHGEYAPSSNPFAYAFLGHYIDGMSMNDYIWMMQRKIILELVQEGPCVIV
                     GRCADYILRDRDDCFNVFICSDLDKKVERIVRLYGETDEKPEKRLADKDKKRRANYKY
                     YTDQVWGLAQNYHLCLNSGEIGIDKCTELIVDLVK"
     gene            10042..10785
                     /locus_tag="PLK68_00520"
     CDS             10042..10785
                     /locus_tag="PLK68_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM88922.1"
                     /translation="MDGTLLDHDTHLMPSSTFEALKKLRKNGIRLFVATGRPPNNLKV
                     IQDCFEFDGFLTSNGQYCFNHEVVIHEKYIEREDIRNLLPYINKNQIPVLFVEINGNY
                     SNIQNYRLGEAARSLNKEGYPIKAVNEIIESKIIQLMAYIDETKDSELLSYMPNCKSA
                     RWTSLFADIIPIDGGENKGIDQMIKHYQINLGEVMAFGDGNNDIDMLKHVGVGVAMGN
                     ANDLVKAASDFVTDTINDDGIFKALKNLN"
     gene            10804..11469
                     /locus_tag="PLK68_00525"
     CDS             10804..11469
                     /locus_tag="PLK68_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM88923.1"
                     /translation="MEQELTKFLTFWDHLNENEKELLKQNATLQTYAKGITMHRGSDD
                     CLGVVLVRKGQLRTYMLSPDGRDITLYRLFSGDVCILSASCVLETITFDVFIDIEENS
                     EIITITATLFQQLAKQNIYVETFGYKMATNRFSDVMWAMQQILFMSVDKRLAIFLKEE
                     AEKNNSLELKITHEQIAKYMATAREVVTRMLKYFSQEGIVKISRGKITIVDKHKLDKL
                     TID"
     gene            11644..>11810
                     /locus_tag="PLK68_00530"
     CDS             11644..>11810
                     /locus_tag="PLK68_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM88924.1"
                     /translation="MLRKDFLWGGAVTAHQSEGGYTLDGKVPAVCDLTVTGEYSDFKD
                     GIDSYHRYEED"
BASE COUNT         4088 a         1523 c         2113 g         4076 t
ORIGIN      
        1 ttgattccat ttttcaaaaa agcattttgg cggtcaatac aagtaccaca tgtgccgcaa
       61 ggggtatcat ttccttcgta acatgaccaa gttaattgat aaggtacgcc taatgaaaga
      121 ccgattttaa caatatctgc tttggtcatt tcaacaaaag gggctttgat ttccaattga
      181 tggccactgc cttcataaat agcttggttc atcgcattat taaaaacttt actacaatca
      241 ggatatgcgc ttccagctgc atcatcacta tgagcaccat aataaataat attacagcct
      301 ttagaaaggg caatgctagc agcgctagat aaaaataaac catttctaaa aggcacataa
      361 gtagaaacag gattgccatc tgttttattt aattgctcat tgtacgattc gtggggaatc
      421 tcattatctg aatgttgtag taatgagcaa ttactataag tgaagatttt tgctaaatct
      481 aaatacaagt gttcaacttg ataaaattgg gcaattttat ttgcgacttc tatctctttg
      541 gtatgttttt gaccataact aattgaaaga gcaactacat tgtcctttcc gtgttcttgg
      601 attgccagtg ctaatgcagt ggtggaatca actccaccac taaataatac taatgctttc
      661 atcttactac tccttaatgt aaataaattt gcagcttact atcagtttta aaacgaccgg
      721 ttagagtact tgtgacagta gtgctattaa cttttttgat tccacgagat gacatacagc
      781 tatgtttacc ttcaatgatt acagcgatat ctttagagtt tgttactaga ctaataattt
      841 cagcaatatc tgttccgatt cgttcttgaa gttgtagacg tctcgctgcc atatcagcaa
      901 tccgtgcaat tttactaaga ccaattactt taccacaagg aatataagca accgtaactt
      961 tcatgtcata cattaaagcg aggtgatgct cgcaataact aaagatatca atatccttga
     1021 ttacaacaac ctcttgatta tcgaagtcta aatcgtcaac aaatgtttta tcaaacatcg
     1081 ttgcaatttc tagattacta tagttcatnn nnnnnnnngt ctaaaatgat tgatactaaa
     1141 aaaatagagg aacacatcta cggtatcttt taatccttcg cgttctggat catcacctaa
     1201 ggctttaaga ataccgtaga tgtgttcctc tattttttta gtatcaatca ttttagacac
     1261 ctctttgatt aatatcccaa atgaatttgt gcatttgtag ttgcatatta ataccattta
     1321 gatgatgttt gaccatataa tcgacgatca tccgtggttc aatcttacca aagactgggc
     1381 taaaataaac tttggtacga ttgacaaggt cataagtatc aattatttct tttgctttat
     1441 ttaaatcact gagattgcta actacaaatt taacaacatc atttttagtc aaatattgat
     1501 agttatttaa gcacatttca ttttccattg tactgctagg tactttataa tctaaagtaa
     1561 agattggtcg agtttcttta ataaagggtt taatatcaat actaccatta gtctcaattt
     1621 caacactaaa accattttta agcagtaaat caattaaata atcaatattt tgttgaagta
     1681 atggttcacc accagttaaa gtgacattaa cgactttatt tgcttttaaa taatctagga
     1741 tattttgagc acttaaaagt tcataattgc tatttgaatc attagcatac ctagtatcac
     1801 aataactgca atttaaattg caataatgaa aacgaataaa ggcggctaat tgacctgctc
     1861 ggcttccttc accattaata ctgataaatt tttcaacaac tttataatta ttcataatta
     1921 acctcgataa cttgcacagt tatttggggt ttcatatact tcaagacaat caactagata
     1981 atctttactt aaagcctcat aaatatactt agctaaattt tctgcagttg gacgaaatgg
     2041 taagttgatt attttgaaat tttcatcgat cagtgcttgg tatagttttt ctgataaact
     2101 gcctgtttca ataattaaag catgatcaaa actatctgcg ataattttta aatctttttt
     2161 taaatctcca aaatcagtta ccattccttt attttgggga tcttctagca gtttttcact
     2221 tttgatagtt gcaatgactt tccagcgatg accatgaata ttagcacatt taccatgata
     2281 accagctaaa aaatgtgcac tatcaaaact ttgttccgtt tttaaaaaat acatctttcc
     2341 tccttctata ccatcaaata aaaaagcagg ctcactaaaa agcctgctta aaaacaacat
     2401 aaaaagcata gccctagttt tgtttataga caggatggta caaatgaact gtctatagta
     2461 atagtatctt aaattggagg attgtcaaga aaattgaata aacttaataa aatgcgtaaa
     2521 aatatgtaaa gaaaagttaa gaatttgtta acaatctttg cagtatgtaa attttatgtt
     2581 aaagtaaccg tgtaacaggg ggaataagat ggaacttaca gatttctttg ctatgtttgg
     2641 tggtttagcg ctatttttat acgggatgac gatgatgtca aatggtctcg aattagcagc
     2701 tggaaacaaa atgaaaacaa ttttagaaaa acttacgacg aatcgctttc taggcgttgg
     2761 agtaggagca gttattactg cagtaatcca atcatcatct gcaactacag tcatgactgt
     2821 tgggtttgtt aatgctgggt tgatgaaatt ggaaaatgcg gtttgggtaa ttatgggtgc
     2881 taatatcggt actacaatta caggtcaatt gatcgcaatt gatattacag ctttagcacc
     2941 agttattgca tttgtcggtg ttgctttaat tgcttttttc aaaagtaaaa aacttgatgc
     3001 aattggtggt attattgcgg gactcggaat tctctttatg ggaatggaaa tgatgtctag
     3061 tgcgatggta ccgcttagaa gttcaccgga atttgttaat cttgtcacta cgtttgaaaa
     3121 tccattaatc ggtattttag ttggtgctgg attcacagcg ataattcaaa gttcttcagc
     3181 ttcggttggg attttgcaag ctttagcgat gagtggcgta attactttac catcagcaat
     3241 atatgtatta tttggtcaaa atattggtac atgtattact gccgttttag ctagtattgg
     3301 aacagggcgt aatgcaaaac gtacaacgat cattcatctt tcatttaata ttattggaac
     3361 gatcgtcttt gtttttatta gtatgttaac accgtttgca tcatttatgc agtcattgac
     3421 gccaacaaat attccggcac aaattgctaa tgtacatacg gtattcaatg ttgttacaac
     3481 attattattg ctgccattcg gggcgcagtt agttaaattg tcatatttgg ttttacctga
     3541 aaaagaaggt tttgaagata aattatctgt taagttcttg gataatagta tctttaccaa
     3601 tgattatcac attggaacta gtgcaatcgc taacacacaa ttatttaatg aaacacaaaa
     3661 tatgttaaat atagttcaaa aaaatgttca aagagctttt gatttaatta ttaagtatga
     3721 tgaaaagaca catgaaaaat tattaaaaga tgaacaatat attgattatt taaacaaaga
     3781 aattattcag tttacaacca atgctatttc taatgaattt ccaatcgatg attctaaatc
     3841 aattggctta ttcttaaaaa cggctggaga tttagaacga gttggagatc atgcggttaa
     3901 tattgctgaa cgtgctgaga aattatatag tgaagatgaa catttttcag atgaagcgat
     3961 gagagaaatt aaaattatga atgacctgac aagaaatatc ttagaggaat taaatgtctt
     4021 aaatcgcgat gaacttcata atattgttga aaaagttgat gttattgaag atagtattga
     4081 tattacaaca catgaatttt cacttaatca gttaagacgt ttgcgagata aaaaatgtac
     4141 gccggaacat agtgcattat atacagaaac attaattgat tttgaaagaa ttggtgatca
     4201 tggtttaaac attgccgttg cttttgatga aataaaagat gatttaacag aaatggctta
     4261 actagctgct tatgcagcta gtttttttat aaaaataaaa tcatattgaa agggtagatg
     4321 atcttattta tttaatagat cggcacgatt attaataaag agttctgtta tttgataact
     4381 ctcagtatct tgataatcaa taggtattat ctcttttgag tcaaaattaa gtatttttgc
     4441 cccagggatt cctaatagga tcggtgaatg actagcaata ataaactgtg agccctgctt
     4501 tgctaattca taaatagtaa ttaataaagt tagctggcgc tggggtgata atgaggcttc
     4561 aggttcgtca agaatatata gactattctg attaaaacga ttttgaatca atgttaaaaa
     4621 actttcacta tgtgattgct ggtgtaatga tttaccacca taatagcgat agggattaaa
     4681 aacatttttt tcatatcatt ctaattgtgt tgcgacatta taaaaacttt ctgcccttaa
     4741 aaagaagcct tcatcaggac gacgataacc acgatcaagg attattgcat gatgcaaaga
     4801 tgaatgagta ttgttagtgt gaaaattaaa gttcaaagtt cccccctcag ggttaaagcc
     4861 atatttaata gcgattgctt ctaataaagt ggattttcct gtgccgtttt ctccaacaaa
     4921 aaaggtaatc ggattatcaa aaatcaaagt atttaattgt agcaattgta tttagataac
     4981 tgtttcgctc gattaaatca tgatcaatag ttatacgatt tataaaatag gtactcattt
     5041 catcacctct agtttaatca taatacaatt tattgtgata atctattttt aattaataga
     5101 tagtacatat tttaatgata taataaaaga ataaataaag gtgggaggat atattatgaa
     5161 gattgatcgg acatatgcaa tttatttttc accaacttac acttccaaga agtcagctgt
     5221 tagtattgct cgtggacttg agggagagtt aagtgaaatt gatttaacac tagaagaaac
     5281 gattccagag atgacattta gtcgtcatga tattgtagtt tttggttttc cggtttacgg
     5341 tggaagaatt cttcacgagg ctttggaacg gttaaaatca tttcggggtg atcatacatc
     5401 atgtgtaatt actgttacat atggaaaccg tcattatgat gatgcattat tagaattatt
     5461 taatactgtt aaggagcaag gatttattcc tattgctggt gcagctttag tgggtgaaca
     5521 tacttatggt caaattcaag tgggtcgtcc taatcgtgat gacttatatc gtgatgaatt
     5581 gtttggtagt ttggtgcggc taaaaattaa agatgataat ttttcatttg tttcagtacc
     5641 agggaaatac ccatataaag aaggaggaac tgggggaaaa tttcgcccag aaactaatga
     5701 acagtgcagc ggctgtgggg tttgcgtcaa tatgtgtcct acaaatgcta ttgatataga
     5761 agattgtaag acaattaata atgattgtat tgcttgtttt agatgtattc gtatttgccc
     5821 agtccatgct aagagtatga ataacaatca agaatatcaa acatttgcag aaagctttag
     5881 caagaaactg gcaacaccta gggaaaatga atactttatt taaatttttg agttttttat
     5941 taagctttaa gagattttaa aatgtcagaa agataggctt ttatgaaact ggtgagaaaa
     6001 ttattttctt gctagttttt ctatttgtaa tctatgtata atatatctat gaagattaga
     6061 cggaggaaat aataatgaaa aaattaaatt ggggaattat tggcagtggt gtaattgcta
     6121 atgaaatggc tcaggctttg ttggatgtaa atggggagat atatgcggtt ggacatcgtg
     6181 atatgaataa agcaattgat tttgcaatga aatataaaat taaaaatgct tatggaagtg
     6241 ttgaggaatt attaaatgat cctgatgttg atgttgttta tattgcaaca ccccataata
     6301 gtcattatga aatcatgaaa caggctgtag cagtgaaaaa gcatgtttta tgtgaaaaag
     6361 caattactgt aaatgatcgt caattggaag aaattgttgc tttagctaag aaaaataatg
     6421 ttgttgtaca agaggctatg acaattttcc atatgccgct atacaaaaaa ttaaaggaaa
     6481 tggttgctgc aggagtaatt ggaaatgtaa aaatgattca agtcaatttt ggcagctgta
     6541 aagaatatga tgttaataat cgtttctttt caaaagaatt agctggtggg gcattattag
     6601 atattggtgt ttatgcaaca agttttgccc ggtacttttt atcatcaaaa cctaatgttg
     6661 taattacaac tgctgattat tttgaaactg gggttgatga acaatcagga atcattatga
     6721 aaaatcaaga tggtcaaatg gttgtaatgg ctctaacaat gcgtgcaaaa caaccgaagc
     6781 gtggggtcat atcaggagaa ttggggtata tagaaattaa taattatccg cggagtaatc
     6841 aagcaacgat aacttatact aaagatggtc atcaggaagt cattgaatgt ggtgatgata
     6901 aacagggact acaatacgaa gttattgata tgcaagatta tattataaat aatcttggtg
     6961 aatatgaatt acagttaact cgggatgtaa gttcactgct aagtcaaatt cggactcaat
     7021 ggggaatgat ttatccattt gaataattta acatttaaga ggggatgatg attaagaatt
     7081 attatgcatg attagtgtgc tgatttttgc tgatattttc attgttacat ttattagtgt
     7141 ggcacttcaa catcaaatga aagctctaaa aatatatcga cttataaaaa acaattgtta
     7201 gttttagata gcagtgataa tttttggaat gaggaacgaa taaaaaaaag tagaaaatcg
     7261 ttattatgcg atttaaatgt catggtcaaa tcaagatcat gaaacattaa aagattattt
     7321 gcccccacat tgttagaaaa atgggaaact aagaaggaac gtataaaaaa gattaaatta
     7381 ttggatatgg ttgttgaact atacgataga aaatgataat gaggattgtt tgtgggctta
     7441 tattgaggag aaaatagagt aattacgtgt gaaacaaaag cttttgtaga atactggaaa
     7501 tttataagaa atagtaataa tatatgttta gataagtttg aaaattgatt ggaaagaatt
     7561 gttatgaaaa gttcaaatca aaatttgaat atagtaatag ggtgtttgaa aaggtgctat
     7621 ttaattataa aatttatcaa ttttgtataa gtagtcttgg gaaaactgtg agcaggatag
     7681 ttttatgata tattatttaa attttgaata ataaagaata gacgtgtgaa atattcgcac
     7741 gtctatttat atagatttgg tttgattatt ttgttatgaa tgatgcgttc aatatattcg
     7801 tatacagtaa tactgcgatc gttcattgct aaaactaaat ggtagggatc tttaaagtct
     7861 tttacatgac ttttaagaag attagctaac tcaataatat gctcattatt tgaagttaaa
     7921 tctgttttaa taaccatctg ttcattaatc ttaaaataaa ttggatgagc gacttcactt
     7981 ccaaatgagt gtattctaaa tgtttgatta taccatccaa tttcacattt tttaatatcc
     8041 ttaaaggcaa ttttatcttg atacatttct ttattaaaaa gcgtattaat aattaattta
     8101 aggatattaa aataacttgt tgatttataa taatatatat attgattatc atatccatgc
     8161 tgacggtaaa ataacgctct tacagtgaag atgaagtaag tgataaagaa aattattcca
     8221 attgtaatga tggctggagc ccaataagga ctatttaaat ttttggccca agtactttta
     8281 gctgcgagta agacaatgat cagcatgata ctccataaac cagctaataa taataaagat
     8341 aagatttttt gtttagatgc ttttgtagat agtgttttca tttttttagt ttcctttctg
     8401 taaaagaaat agcatgtttt tttaacacta caagtaaatc ttgatatctt cttcttgaaa
     8461 tagcaatatc tttattttcg tagtttttca taattacatt ttcactgttt aaagtaatta
     8521 atctaaagat attgataatt gctgaacgtg agattttaaa aaaggttttt ggaagcattt
     8581 tttgtagata ttcaaagttt tgaataaaaa tgcgatatgt tttgtctaat gtgtttagat
     8641 atgtcatgcg atttaaatat tcaaaataaa caatatcatt tgtttgggca ttgacgatgc
     8701 cctgatcact tttaaatagt agtaattttc gtttatcaag ataatcgatg atatcttgaa
     8761 ttgctggaac aaatttcgct ttaatatctt gatagcgcag atagctggaa atattaatac
     8821 ctactgcatc gaaaatagtt gaaccgtctt ttgagataat tagtacaagc atatcattat
     8881 tattttcaat taatgtttta gcataattga ttccttgatt atcaaaagaa tcttcaataa
     8941 tacagaaatc catttttttt gtaaataaca tttccttatc atgaatctca atgatacgat
     9001 attcactgtc aatattttta atgattagtt cctttaattg tttgatcaca ctattatcaa
     9061 caaccattaa tccaattttt ttcatttaga aatctccttt attacttgca gttatctttt
     9121 acacctttat tataggggct tacttttagt atattttaat tattgtatga atggtataaa
     9181 aaatatcttt tggacaataa aattagtgat ttctcattac ttttcgcttg taaagtagta
     9241 aaattaaagt atagataatg actaagatta ggaggaggtg cagatcatgc gcaagaacat
     9301 tattacaatt agccgtgaat ttggtagtgg cggtagaaca attggtaaag aggtagcaaa
     9361 acgattaaac ataccttttt atgataagga attaattgaa aaagtagctc gagagagtgg
     9421 attaaatgtg aattatattg aagaacatgg tgaatatgca ccatctagta atccttttgc
     9481 ttatgctttt ttaggtcatt atattgatgg gatgtcaatg aatgattata tttggatgat
     9541 gcaaagaaag attattttag aattagtgca agaaggacca tgtgtaatcg ttgggagatg
     9601 tgcagattat attttaagag atcgagatga ttgttttaat gtctttattt gtagtgatct
     9661 tgataaaaaa gtggaacgga tcgtcagatt atatggggaa acagatgaga agcctgagaa
     9721 acgtttagct gataaagaca aaaaaagacg agcaaattat aaatattata ctgatcaagt
     9781 ttgggggtta gcgcaaaatt atcatttatg tttaaatagt ggtgaaattg gaatcgataa
     9841 atgtactgag ttgattgttg atttagtcaa atagtagtta agagaaagta actattactt
     9901 tctcttttta tgaattgaag gattacatat gctatactgt attggaaaac attataataa
     9961 aaagacgatt attttattat gttaagaact aaaagataaa ataatatgag gataagtaga
    10021 tgataaaagc aattttttga tattgatgga acactattgg atcatgatac acacttaatg
    10081 ccatctagta cttttgaagc attaaaaaaa ttacggaaga atggaattag attattcgtt
    10141 gctactgggc ggccacctaa taatcttaaa gtgattcaag attgtttcga gtttgatggt
    10201 tttctcacaa gtaatggaca gtattgtttt aatcatgaag tggttattca tgaaaaatat
    10261 attgaaagag aagatattag aaatttatta ccatatatta acaaaaatca gattcccgta
    10321 ttatttgtag aaataaatgg taattatagt aatatccaaa attatcgtct tggtgaagct
    10381 gctagatcac tgaataaaga gggctatccc attaaagctg ttaatgaaat aattgaaagt
    10441 aagataattc aattaatggc atatatagac gagactaaag atagtgaact attatcatat
    10501 atgcctaatt gtaaatcagc acgctggaca tcgctctttg cggatatcat ccctattgat
    10561 ggcggagaaa ataagggaat tgatcaaatg ataaagcact atcaaattaa tttaggtgaa
    10621 gtaatggcat ttggcgatgg gaataatgat attgatatgt taaaacatgt tggagttggt
    10681 gttgctatgg ggaatgctaa tgatttagtt aaggcagcta gtgattttgt tactgacacg
    10741 attaatgatg atggcatttt taaagcatta aaaaatttga attaatttga aagggtgaaa
    10801 atgatggagc aagaattaac aaaattctta actttttggg atcatttaaa tgaaaacgaa
    10861 aaagagttat taaaacaaaa tgccactctt caaacttatg ctaaaggcat aacaatgcat
    10921 cgtggtagtg atgattgttt aggtgtggtt ttagtgagaa agggtcaatt gcggacatat
    10981 atgttatcac cagatggacg tgatattaca ctatatcgtt tattttcagg tgatgtatgt
    11041 attctttcgg catcatgtgt tttagaaacg attacatttg atgtttttat tgatattgaa
    11101 gaaaatagtg aaatcattac aatcacggca actttatttc aacaattggc gaagcaaaat
    11161 atttatgttg aaacatttgg gtataaaatg gcaaccaatc gtttttctga tgttatgtgg
    11221 gcgatgcagc aaattttatt tatgtctgtt gataaacgct tagctatttt tcttaaggaa
    11281 gaagctgaaa agaataattc tttagagtta aaaattactc atgaacaaat cgctaaatat
    11341 atggcgacgg ctcgtgaagt tgtaactagg atgctaaaat attttagtca agagggtatt
    11401 gtaaaaatat cacgaggaaa aataacaata gttgataaac ataaattaga taaactaact
    11461 attgattaat tatggaataa tcattatgat tattcttttt tattcaagtt aattaatttt
    11521 ttttcaagtg attatgagtt gaaattcaag ctgacattaa agataataaa tctatatcag
    11581 taatagtaat ttgttatata aaaaatagct atactgctag aaatagaatg ggaggaagaa
    11641 gtaatgttac gaaaagattt tttatggggt ggggcagtta cagctcatca aagtgaaggt
    11701 ggatatacac tggatggtaa agtaccagcg gtttgtgatt taacagttac cggtgaatat
    11761 tctgatttta aagacggaat cgatagttac catcgttatg aagaggattt
//
LOCUS       DAOMTA010000008        11635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_26_length_11635_cov_2.272107, whole genome shotgun sequence.
ACCESSION   DAOMTA010000008 DAOMTA010000000
VERSION     DAOMTA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26_length_11635_cov_2.272107"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PLK68_00535"
     CDS             complement(<1..134)
                     /locus_tag="PLK68_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM88925.1"
                     /translation="MFEKLDDSKKNSEKIAYESKTYIADAWNRFKKNKLALIGLCFLL
                     V"
     assembly_gap    277..329
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<330..1139)
                     /locus_tag="PLK68_00540"
     CDS             complement(<330..1139)
                     /locus_tag="PLK68_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM88926.1"
                     /translation="MFRYVLKRVLIGIVTLFLLSSATFFLMKATPGSPISGEKYKNEA
                     AREIAIKKYNLDKPVFEQYTLYMNDLIHGDLGESIVRPGRDVGTTISQSFPVTARLGA
                     IAFASALVIGVALGTAAALSKRKWLNNLCMFIATIGVSVPSFLIGVLLMIVLGVKLKL
                     FPFVGLTSPAHYVLPALSLAFYPIAMVCRLTRSSMLEVMRQDYIILARSKGTPYKKVV
                     IKHALKNALIPVITYAGPAFAYMLTGSFVIETLFSVPGIGREMVSSIQTRDY"
     gene            complement(1399..1932)
                     /locus_tag="PLK68_00545"
     CDS             complement(1399..1932)
                     /locus_tag="PLK68_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRM88927.1"
                     /translation="MAKKIKLTKTQQKKLLRSWLGIIVILAVIGYNFYQQNKSIPTGE
                     RFEVTLDRCVDGDTAWFNVDGESTKVRFLYIDTPESTKEIEPYGKEASDYTKTQLTNA
                     AKIELELNVDGDSKDKYGRLLAWVFVDGDLLQEQIAREGLVEKFYDYGYDYTYKNEII
                     EAANSAKSMRKGIYSEN"
     gene            complement(1925..2512)
                     /locus_tag="PLK68_00550"
     CDS             complement(1925..2512)
                     /locus_tag="PLK68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HRM88928.1"
                     /translation="MAKYYAVKNGRKPGIYSSWDECKKQVEKFKGAIYKSFTSLEDAK
                     VFIKEEKIEFDGGLIAYVDGSYNIKTHEYGFGCVIIEEQKVIKEMYGKGADENYASMR
                     NVAGEILGSICAMEFAKNNGYKQICIYFDYEGIEKWANGMWKANKPGTQEYQRKVKEY
                     RQDLKIAFVKVLAHSGDFYNERADVLAKKAVGING"
     gene            2679..3887
                     /gene="pepT"
                     /locus_tag="PLK68_00555"
     CDS             2679..3887
                     /gene="pepT"
                     /locus_tag="PLK68_00555"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRM88929.1"
                     /translation="MDIEERFLKYVSIDTQSDEYSDTTPSTLKQLDLGKELVKEMLEL
                     GITDAHLDEYGIVYGTIKGNGGTGDIIGFIAHMDTSPDASGTNIKPQKINNYDGSIIE
                     INKELGLSLDPEEFVSLKKMVGHDLITTDGTTLLGADDKAGVAIIMDLANYLYEHPEV
                     KHNDIKIAFTPDEEVGRGADNFDVNKFGAKYAYTLDGGDIEEYNYENFNAYSAQVEIT
                     GKSIHPGNAKDKMVSAINVAIEFENMLPAQQKAYFTDGYDGFNHLHHLEGGCEKATLE
                     YIIRNHDLTLAKKQINDFKRIKKYLDEKYGYELIKLDIKESYLNMAEVIKENYYIIER
                     LEKAMNSVGIQGFASPIRGGTDGARLTFMGLPCPNIGTGGDNFHGPFEFVSLTMMKQS
                     VEILKELIKE"
     gene            3892..4788
                     /locus_tag="PLK68_00560"
     CDS             3892..4788
                     /locus_tag="PLK68_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRM88930.1"
                     /translation="MKRCLFIINPSSGQRTIQNTLDKLIGQLTLRQLINHIDVFYTLK
                     KDDAYFKALNADEQDYDFITVVGGDGTINEVVSGMVASDKKIPLCILAAGTVNDFANY
                     LNLPSNIEGVCNLINNFKTVCCDVGKINERYFMNVAAGGMFSDVSFTVSKADKKRLGP
                     LAYYLNGIANLPSQLNTNINLKIVLDDANILETEAKMFMVTNTNRVGGFENIIPYADI
                     QDGMLDLIVIKKCSVTDLVALSKDYLLKKHANSPFISYVQAKKIEIYSQQKVVIDIDG
                     EEGSPLPVTIEAISQAINILVP"
     gene            4872..6170
                     /locus_tag="PLK68_00565"
     CDS             4872..6170
                     /locus_tag="PLK68_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRM88931.1"
                     /translation="MHPKYHLTAPKHWINDPIGFIYYQNNYHLFYQHFPYENKWGTMH
                     WGHATSKDLVNWVDQGIALYPSKDFDANGCFSGSALEVDKKMYLYYTSVVYQKVNPEN
                     IHKTAPGYDFYSSQSMIISDDGFNFDNLNQKQLIIPVFKDGEVGHPVHTRDPKVWKFQ
                     DTYYMVLASKYLDDNNDYNGQLLFYTSKNATEWHYANNYRGIKCGDMWECPDIFTVAH
                     QDILIMSPERTNDSGYPSHARITTANFDHQNCQLEITGELNYLDYGLDIYAPQTTIDE
                     AGRRIYVGWMRMPVADEANWIGLITYPRVITYQNDAIFTNIHPSVDSLFKKPATEFKA
                     TQACKIVTNLKTGDFINIGGYLIKYDDCLCIDRSNVFKSDAALKELRSPKIDHCNLNI
                     YYDQDIIEIYINNGQYVLSNIVYEMADTIKSNTDFEIFTD"
     gene            complement(6429..6608)
                     /locus_tag="PLK68_00570"
     CDS             complement(6429..6608)
                     /locus_tag="PLK68_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRM88932.1"
                     /translation="MKRIYRSRQDRMVCGVCGGIAEYFDLDPSLVRLGWIIFSAMGGS
                     GFIAYIIAAIVIPSR"
     gene            complement(6612..7397)
                     /locus_tag="PLK68_00575"
     CDS             complement(6612..7397)
                     /locus_tag="PLK68_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRM88933.1"
                     /translation="MKKIGYVALGSLLLAIVLFISGTMIGGFSELETLYDKGDFTISL
                     PITKTMDVTKEFTDIKNLEIQAEAGTVELIEYEGSTIKVEAKNVSKKIKLYQEQNTLI
                     VKDSFRFWHFINVTDITTRIKIYIPNSYEFNKVELEVDAGELIVPNLKANDVEIDVDA
                     GSFKAENIIASYTKVDVDAGDARINLLNSYRSEFNCDAGDIDATMVGSESDYSYEVDS
                     DVGDISIGSYRSDGLSDEYSHSGGQRKIEADCNVGSIRIKMEV"
     gene            complement(7394..8125)
                     /locus_tag="PLK68_00580"
     CDS             complement(7394..8125)
                     /locus_tag="PLK68_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88934.1"
                     /translation="MNRKKFIEELAFLLQDIEDAEREEAMQYYEDYFDEAGPENEQQV
                     INDLGSPERVAAIIKAGLDNQFDQDIEYSEKGMDNSNYKQSREIIDAKIISEEETAAD
                     DNNDYQNNKRHKNNFRGNSDRNRILLIFIIIGAVFLALPVGGGILGLGLGFFGAVFGL
                     GVGILCGGAACLIGAIVCFVKAFMIIAAYPGAGLITMAAGCALIALAFVFFWLAKGLI
                     KIIPAVIRGIVDFCQNIFNRVGDRR"
     gene            complement(8128..8445)
                     /locus_tag="PLK68_00585"
     CDS             complement(8128..8445)
                     /locus_tag="PLK68_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRM88935.1"
                     /translation="MIFNTGAALLDAIVLSVVSKEAEGTYGYKITQDVRIALDVSEST
                     LYPVLRRLLKDNCLETYDQEYGGRNRRYYKITSQGQAQLEMYKGEWHEYVRKINKIIE
                     GGK"
     gene            9138..10778
                     /locus_tag="PLK68_00590"
     CDS             9138..10778
                     /locus_tag="PLK68_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HRM88936.1"
                     /translation="MKKVKNILIGLLVFSIIALCFIFNGPLVTEASSDDENSRPYTQL
                     KNTTSDPETIYSDKYQKNIYQQIKKIKQINNYTFNNPLLIENPYGTNTTGIYMYFTTD
                     YQCVATYTISCEGYEDFTRTLNTNSLSGFSKEHEYLLIGSIPDAKNTITVTLTDTNGK
                     QVDSITWSYQAPKLSGGDQYLSVATESYDTTSALSNGLYTVLGNDVTEENDEQTYMRL
                     YDNDGVIRSEIPIISYRSHRILFEDNTMYLSVSSTKIVGVDQTGYVSTIYSTGDYKLH
                     HDYIFDSQNNLIVLASKKNAVTSEDKIIMIDHNTKAIIELVDLIELFPDYYKTTAKPD
                     SADDLDWMHINSLELVGKDSLIISSRETSTIMKLDNIYSNPTVDYMIGSNNFWQESGY
                     DDLLLTKTSDFSLQAGQHCVTYVEDTTLASGQYYLYLYNNNLAVSTTRSDYNWKEDDN
                     YQNVSYDTKNGISYYYKYLIDENNRTVSLVNTIPVDYSGYVSSVQELDNNIIIDSGMA
                     MSWGEYDQFGTLIKKFTTTGGKFVYRVFKYDYNDYWFQ"
     gene            complement(10869..>11189)
                     /locus_tag="PLK68_00595"
     CDS             complement(10869..>11189)
                     /locus_tag="PLK68_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM88937.1"
                     /translation="ARWGKKGFELPADHPAWDLEAYYIGQALATYVLVISPKKIILGG
                     GVSKQKQMFPLIHKYLREFLNGYIQKDEILTDKIDDYIVSPALGDNAGVCGALALAKQ
                     ALEK"
     assembly_gap    11190..11199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11200..>11635)
                     /locus_tag="PLK68_00600"
     CDS             complement(<11200..>11635)
                     /locus_tag="PLK68_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM88938.1"
                     /translation="KDIEALGVGCFGPIDPDLNSPTYGYITTTPKPGWGNFNIMGALK
                     ERYDIPMGFDTDVNGAALGEAYFGAAKGLDSALYMTIGTGIGCGAIVEGNLVHGLLHP
                     EMGHMNMIVREDDTYAGKCPFHGTCFEGLAAGPAIEERWGIKG"
BASE COUNT         3898 a         2125 c         1643 g         3906 t
ORIGIN      
        1 accaataaaa agcataaccc aatcaatgct aatttatttt tcttgaaacg attccaagca
       61 tctgcaatat atgttttact ttcgtaagcg attttttcgc tattcttttt cgaatcatct
      121 aacttctcaa acatttctgg agtaagttcg atgttcatta ccttattttc ttccatcttg
      181 cttcctccta ttttaatttg attcgtggat ctactactgc tgatagtaaa tcagtaataa
      241 tattaaatga aattaccaag aatcctaaga atattgnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taatcacgag tttgaatact tgaaaccatt
      361 tctcgtccaa ttcctggtac tgagaataga gtttcaataa cgaaacttcc tgttaacatg
      421 taggcaaacg caggacctgc atatgtaata actggaatca atgcattttt taacgcatgc
      481 ttaataacga cctttttata aggtgttcct tttgaccgcg ctaagataat ataatcttga
      541 cgcataactt caagcatact acttctagtt aaacgacata ccatggcaat tggataaaac
      601 gccaatgaca aggctggtaa aacataatga gccggtgaag tcagccccac aaatgggaac
      661 agcttcaatt taacccccag aacaatcatt aaaagtaccc cgatcaaaaa tgatggtaca
      721 gataccccga ttgttgcaat aaacatacaa agattgttaa gccatttacg ttttgataat
      781 gcagctgcag tacctagtgc aactccaatc actagtgctg aggcaaaagc aattgcacct
      841 agtctcgctg taactgggaa actttgtgag attgttgtac ctacatcacg tccaggacga
      901 acaatacttt cccctaaatc accatgaatc aaatcattca tgtatagagt atattgctca
      961 aatactggct tatctaaatt gtacttttta atcgcaattt cgcgtgccgc ttcattttta
     1021 tatttttcac cactaattgg cgaccctggt gttgccttca tcaaaaagaa tgttgctgat
     1081 gaaagtaaga acagtgttac gataccaatg agcacccgct ttaaaacata cctaaacatt
     1141 taatcaccta ttcctttact atatatctac taggatacct taatatgtat caaatttcaa
     1201 acattttttt acacgctaac cccatgagcc cctagggatt ccgcccaaaa accgcaaatt
     1261 gtatacgatt atacacaatt tgttggtatt aaaatttttt tataaatacc atactttaat
     1321 tgatttctgc gataattata ccaataattt attatcaagt caataattta tttaattttg
     1381 tattaaagcg catacaattt aattttcaga ataaatccct tttctcatac ttttagcact
     1441 atttgctgcc tcaataattt catttttata agtgtagtca tatccgtaat catagaactt
     1501 ttcaactaaa ccttcacgag caatttgttc ttgaagtaaa tcaccatcta caaagaccca
     1561 tgctaataaa cgcccatact tatctttact atcaccatct acatttaatt ccaattcgat
     1621 tttcgcagca ttcgtcaatt gtgtttttgt ataatcgctc gcttctttac catacggctc
     1681 tatctcttta gttgattcag gtgtatcaat atataaaaaa cgaacttttg tactctcacc
     1741 atctacattg aaccatgctg tgtcaccatc aacacatcta tctaaagtta cttcaaatcg
     1801 ttcgcctgtg ggaattgatt tgttctgctg ataaaaattg taaccaatta ccgccaagat
     1861 aactatgatt ccaagccatg atcttaataa tttcttttgt tgtgtttttg ttagtttaat
     1921 cttcttagcc attaattcca accgcctttt tcgctaatac atcagctctt tcattataaa
     1981 aatcaccact atgggctaat actttgacaa atgcaatttt taaatcttga cgatactctt
     2041 ttactttacg ctgatattct tgtgttcctg gtttgtttgc tttccacata ccatttgccc
     2101 atttttcaat tccctcgtaa tcaaaataaa tacaaatttg tttataacca ttgtttttag
     2161 caaattccat tgcacaaata ctgcctaaaa tttcacccgc aacattacgc atcgaagcat
     2221 aattttcatc agcaccttta ccatacattt ctttgattac tttttgttcc tcaataatca
     2281 cacatccaaa accatattca tgtgtcttaa tattgtagct gccatcaaca taagcaatta
     2341 aacccccatc aaattctatc ttttcttctt taataaatac ttttgcatcc tctaatgaag
     2401 taaatgattt atatattgct cctttaaatt tctcaacttg tttcttacat tcatcccaag
     2461 atgaataaat tcctggtttg cgaccatttt ttactgcata atatttagcc ataacttctc
     2521 ctttaataat tgataaaatg attttaaact agaaattcta tttagtaaag aaacttaaag
     2581 ggtactaaag taagaaaaat taatttcctt agccctcaac agttgtctat ttatcaataa
     2641 tgagtataat taatgtatta ggttaaagga gaaataatat ggatattgaa gaaagatttt
     2701 taaaatatgt aagcattgat acacaatcag atgaatactc agatactacc ccttcaacgc
     2761 tgaaacaact agatttagga aaagaattag ttaaagaaat gttggaatta ggaatcacag
     2821 atgcacattt ggatgaatac ggtattgttt atggaacaat taaagggaat ggtggtaccg
     2881 gggatatcat cggctttatt gctcatatgg atacttctcc agatgctagc ggaactaata
     2941 ttaaacctca aaaaatcaat aattatgatg gttctatcat cgaaatcaat aaagaattag
     3001 gactttcttt agaccctgaa gaatttgtta gtctaaagaa aatggttggt catgatttaa
     3061 tcactactga cggtactact ttattaggag ctgatgataa agctggtgtt gctatcatta
     3121 tggatttagc aaattatttg tatgaacatc ctgaagtcaa acataatgat attaaaattg
     3181 cttttactcc tgatgaagaa gtcgggcgtg gagctgataa tttcgatgtt aataaatttg
     3241 gagccaaata tgcctatact ttagatggtg gggatattga agaatataat tatgaaaact
     3301 ttaatgcata tagtgctcaa gttgaaataa ccggaaaatc aatccaccca ggaaatgcca
     3361 aagataaaat ggttagtgca atcaatgttg caattgaatt tgaaaacatg ctgccagctc
     3421 agcaaaaagc atattttact gatggttatg atggtttcaa tcatcttcac catttagaag
     3481 gtggttgtga aaaagctact ttagaataca tcattagaaa tcacgatctt acattagcca
     3541 aaaaacaaat caatgatttt aaacgtatta aaaaatattt agatgaaaaa tatggatatg
     3601 aattgatcaa actagatatt aaagaatcat atttaaatat ggcagaagtt atcaaagaaa
     3661 attattatat aattgaaaga ttagaaaaag caatgaatag tgttggaatc caaggttttg
     3721 ctagtcctat ccgtggtggt accgatggtg ctcgcttaac atttatgggg cttccttgcc
     3781 caaacatcgg caccggcggt gataatttcc atggtccatt tgaatttgtt tcactcacga
     3841 tgatgaaaca atcagttgaa attttaaaag agctgattaa agagtagttt tatgaaacgc
     3901 tgtttattca ttattaatcc gtcttcggga caacggacta ttcaaaacac tttagataaa
     3961 ttaattgggc agcttacttt gcgtcaatta attaatcata ttgatgtttt ctacacctta
     4021 aaaaaagatg atgcttattt taaagcacta aatgctgacg aacaagacta tgatttcatt
     4081 acagtagttg gcggtgatgg aacgatcaat gaagtagtta gcgggatggt tgccagcgat
     4141 aaaaaaattc cactatgtat actggcagcg ggaactgtca atgacttcgc aaattatttg
     4201 aacctaccta gcaatatcga aggagtttgt aatttaatca ataattttaa gactgtctgt
     4261 tgcgatgtag gtaaaatcaa cgaacgctat tttatgaatg tggctgccgg gggaatgttc
     4321 agtgatgtat cattcaccgt tagcaaagct gataaaaaaa gattaggtcc cttagcttac
     4381 tatctaaacg gtattgctaa tcttcctagt caattaaata ctaatattaa tttaaaaatt
     4441 gttttagatg atgctaatat tttagaaaca gaagcaaaaa tgtttatggt tacaaataca
     4501 aatcgtgttg gcggatttga aaatattatc ccttatgcag atattcaaga tggaatgctt
     4561 gatttaatcg taataaaaaa atgttctgtt actgatttag ttgctctatc aaaagattat
     4621 ttgttgaaaa aacatgctaa tagcccattt atttcttatg ttcaagcaaa aaaaattgag
     4681 atttacagcc aacaaaaagt tgtaattgat attgatggtg aagaggggtc accattgcca
     4741 gtgacaattg aagcaataag ccaagctatc aatattttag taccatagga gtttgttaaa
     4801 cagactcctt ttttattgtt tgattattta atttatatta taatctatct gtacatcaag
     4861 gaggaaatta catgcaccca aaatatcatt taacagcccc taaacattgg atcaatgatc
     4921 ctatcgggtt tatttattac caaaataatt atcatttatt ttatcagcat tttccttatg
     4981 aaaataagtg gggaacaatg cactggggtc atgctactag taaagaccta gtaaactggg
     5041 ttgaccaggg aattgcatta taccctagta aagactttga tgcaaatggt tgtttttctg
     5101 gttcagcatt agaagttgat aaaaaaatgt atctatatta tacgtctgtt gtttatcaaa
     5161 aagtcaatcc cgaaaatatt cataaaactg ccccaggtta tgatttttat tctagccaaa
     5221 gtatgattat aagcgatgat ggatttaact tcgataatct taatcaaaaa caattgatca
     5281 ttccagtatt taaagatggc gaagtcggtc atcctgttca tacacgtgat ccaaaagtat
     5341 ggaaattcca agatacttac tatatggttt tagcaagtaa gtatttagat gataacaatg
     5401 attataatgg tcaattatta ttttatactt ctaaaaatgc aactgaatgg cattatgcta
     5461 ataattatcg cggcattaaa tgcggtgata tgtgggaatg cccagatatt tttacagttg
     5521 cccatcagga tatattgatc atgtctccag aacggactaa tgatagcggc tatccatctc
     5581 acgcacgtat tacaacagct aattttgatc atcaaaactg tcaattagag attactggtg
     5641 aactaaatta tcttgactat ggtttagata tttatgctcc ccaaacaacg attgacgaag
     5701 ccggacgaag aatttatgta ggctggatgc gtatgcctgt tgccgatgaa gctaactgga
     5761 ttggattaat tacttatcca cgtgtaatca catatcaaaa tgatgctata tttacgaata
     5821 ttcatccatc tgtcgatagt ctctttaaaa agcctgcaac tgagtttaaa gctacgcagg
     5881 catgcaagat cgtgactaat ttaaaaacag gtgatttcat aaatattgga ggttatctta
     5941 tcaagtatga cgattgtctt tgtatcgatc gttctaatgt gtttaaatct gatgcggcat
     6001 taaaagagct acgttcacca aaaatcgatc actgtaattt aaacatctac tatgaccagg
     6061 atattattga aatttatatt aataatggtc aatatgtttt atcaaatatc gtctatgaaa
     6121 tggcagatac gattaagagt aatactgatt ttgaaatctt tactgactag aacttgtttt
     6181 aaacaggttc ttttagttta aaaataactt attatggcaa atattgctga tttaaaaccg
     6241 ttttatactt ctgtgacccc aatttattaa attttcagaa aaactcctca aattcaacaa
     6301 aatcagtcat ttaaggtagg tatattatta taatcatttc aatattttat gaagacaact
     6361 ttgattaaaa aattattctt tttaccaaaa taataaaagc gaattctctt gagaactcgc
     6421 ttttgccttt atctgctggg aattactatt gcggcaataa tataagcaat aaagccactg
     6481 cctcccatag cagagaaaat aatccaacct aatcttacca atgaaggatc aagatcaaag
     6541 tattctgcaa ttccaccaca tactccacaa accatccgat cttgtcgtga tcgataaatt
     6601 cttttcattt cttacacctc catcttaatt ctaatacttc caacattaca atcagcttct
     6661 attttacgtt gaccgccact atgactatat tcatcactca gtccgtcact tcgataactt
     6721 ccaatactaa tatcaccaac atcactatcg acttcataac tgtaatctga ttcactacct
     6781 accattgttg catcaatatc tccagcatcg cagttaaatt cacttcgata actattcaac
     6841 aagttaatcc gtgcatcccc ggcatcaacg tctactttag tatatgatgc aataatattt
     6901 tcggccttaa aacttccagc atccacatca atttcaacat cattagcctt caaattagga
     6961 acaatcaatt ctccagcatc tacttctaat tcaaccttat taaattcata gctattcggt
     7021 atataaattt taattctagt tgttatatcg gtaacattta taaaatgcca aaatctaaaa
     7081 ctatccttta caatcaaagt gttttgttct tgatataatt taattttttt gctcacattt
     7141 tttgcttcaa ctttaattgt actaccttca tattcaatta attcaacagt tccagcttct
     7201 gcttgaattt ccaaattttt aatgtctgta aattcttttg ttacatccat cgttttagta
     7261 ataggtagac tgattgtaaa atcaccctta tcataaagag tttccagttc tgaaaaacca
     7321 ccaatcatcg taccgctgat gaacaataca attgcaagca acaacgatcc taatgccaca
     7381 taccctattt ttttcatcgc cgatcaccta ccctattaaa tatattttga caaaaatcaa
     7441 cgattcctct aataacagca gggattattt taatcaaacc tttagctaac cagaaaaaga
     7501 cgaatgctaa agcaattaaa gcacatcctg ccgccatggt aatcaatcct gcgcctggat
     7561 acgctgcaat aatcataaat gctttgacaa agcagactat cgcaccaatc aaacaagcgg
     7621 ctccaccaca caaaatacca actccaaggc caaatacagc tccaaaaaaa cctaatccta
     7681 aaccaagaat cccaccacct actggtaaag ctagaaaaac agccccaata atgataaata
     7741 ttaataagat tcgatttcgg tcggaatttc ctctaaaatt attcttgtga cgtttattat
     7801 tttgataatc attattatca tcagcagctg tctcctcttc actaataatt ttagcatcaa
     7861 taatttcacg agattgttta taatttgaat tatccatccc tttttcacta tactcaatat
     7921 cctgatcaaa ctgattatct aaaccagctt taatgatcgc tgcaacccgt tcaggagagc
     7981 ctaaatcatt tataacctgc tgttcatttt ctggaccagc ttcatcaaaa taatcttcat
     8041 aatattgcat agcttcttct cgttcagcat cttcaatatc ttgaagtaaa aatgctagtt
     8101 cttcaataaa ctttttacga ttcataatta tttcccccct tcaataattt tattgatctt
     8161 acgcacatac tcatgccatt cccctttata catctcaagt tgagcttgtc cttgtgacgt
     8221 tattttataa taacgtcgat ttctcccacc atactcttga tcatatgttt ctaaacaatt
     8281 atcctttaat aatcttctta aaacaggata caaggtcgat tctgaaacat caagagcaat
     8341 tctaacatct tgtgtaattt tgtaaccata agttccctcg gcctccttgg aaactactga
     8401 aagaacaatt gcatctagca atgcagctcc tgtattaaat atcataagcc cagctccttt
     8461 catatttatc tttcaataat attatatgct atatagtatt atctgtcaac taataatata
     8521 taataattat atattataaa aaaagaaaca tcatttgatg tttctcatta gttacaggta
     8581 tgtactcctt ccttaaattt ctttaaagtt tttgttattt ctaacataca tatccaatat
     8641 atgtataaat aatttttcct ctatcagtaa tttcattgtt ctaaatgtct catagctcaa
     8701 ataaaacgct ataaccaaca cgattcacct ccaaaacatc gcattgattc tcaattgccc
     8761 aaacttttaa taacactatc caatatttat cacatcccaa gcgattacta taattaagct
     8821 gcttgtatat gaagccattt gaaaattctg gagaatttta gattataaca aataaaagca
     8881 ctaatatctt atccattctc aaaatttaat ttcactgccc atctgatgat attccgctta
     8941 tttaaaataa gatgtatttc atcgtaactg aataattcct tttcatgatt tacaatcatc
     9001 aaattttaaa attatgacat tgcaattttc atcaacgttt tcccataggc tattaatttt
     9061 atcactttgt taattattgt tttttttaag atatttttca catattcatt tttaataata
     9121 ttttcaagga ggtaataatg aagaaggtta aaaatatatt gattggacta ttagtattta
     9181 gcattattgc gttatgcttt atctttaacg gtcccttagt taccgaggcc tctagtgatg
     9241 atgaaaatag tcgtccctat acacaattaa aaaataccac tagtgatccg gaaactattt
     9301 acagtgataa atatcaaaaa aatatttatc agcaaattaa aaagattaaa caaataaata
     9361 attacacttt taacaatccc ttgttaattg aaaatcctta tggaacaaac accacaggaa
     9421 tctatatgta ttttacaact gattatcaat gcgttgcgac atataccatc agttgcgaag
     9481 gatacgaaga cttcaccaga acacttaata ctaatagttt aagcggcttt tctaaagaac
     9541 atgaatattt gctaatcgga tctattcccg atgctaaaaa cactattact gttacattaa
     9601 cggataccaa tggcaaacaa gttgattcta ttacgtggag ctatcaagca cctaaattat
     9661 caggcggtga tcaatatctc agcgttgcaa ctgaaagtta tgatactaca tcagcgctaa
     9721 gcaacggttt atacaccgtt ttaggtaatg atgtaaccga agaaaatgat gaacagacct
     9781 atatgcgatt atatgataac gatggcgtga ttagaagtga aattcctatc atttcatacc
     9841 gcagtcatcg aattttattc gaagataata ctatgtattt aagtgtttca agcacaaaga
     9901 tcgtaggagt tgatcaaact ggttatgttt cgacaattta ttctactggt gattacaaat
     9961 tgcatcatga ctatattttc gacagtcaaa ataacttaat tgttttagcc agtaaaaaga
    10021 atgcggtaac tagtgaagat aaaatcatta tgattgatca taatactaaa gctatcatag
    10081 aacttgttga tttaattgaa ctctttccag attactataa aactaccgcc aagccagatt
    10141 ctgcagatga tttagattgg atgcatatca actcacttga actagttgga aaagatagtt
    10201 tgatcattag ttcacgagaa acttcaacaa ttatgaagtt agataatatt tatagtaatc
    10261 caacagttga ctatatgatc ggttctaata atttctggca agaaagtggt tacgatgatt
    10321 tacttttgac taagacaagt gacttttcat tgcaggcagg acagcactgt gttacctatg
    10381 ttgaagatac cactcttgcc agcggacaat attatttata tctatataat aataatctag
    10441 cagtcagtac aactcgcagc gattataatt ggaaagaaga tgataattat caaaatgttt
    10501 cttacgatac taaaaacggt atttcttatt attataaata tttaattgat gaaaataacc
    10561 gtactgtttc tttggttaat acaatccccg ttgattactc tggttatgta agcagtgtcc
    10621 aagaacttga taacaatatt attattgata gcggaatggc aatgtcatgg ggcgaatatg
    10681 atcaatttgg tactttgatc aaaaaattca caacaaccgg gggcaaattc gtttatcgtg
    10741 tctttaaata tgattataat gactattggt ttcaataacg caatagtcat tttttaatct
    10801 atgtatagat atttatcata tttaatatac taattaattt aaataaaaag agcctaagct
    10861 ctttttattt atttttctaa tgcctgtttt gctaatgcta gagcaccaca cacaccagca
    10921 ttatcaccta aagcaggtga tacaatataa tcatcaatct tatccgttaa aatttcatct
    10981 ttttgaatat atccatttaa gaattctctt aaatatttat gaattaatgg gaacatttgt
    11041 ttttgttttg agaccccacc acctaagatg attttctttg gtgaaataac taaaacataa
    11101 gttgctagag cctgaccgat atagtaagct tctaaatccc aagcaggatg gtcagccggt
    11161 aattcaaaac cttttttacc ccaacgtgcn nnnnnnnnnt ccctttattc cccatctttc
    11221 ttcaatggct ggtcctgctg ctaaaccttc aaaacacgta ccgtggaatg ggcatttacc
    11281 tgcataagta tcgtcttcac gaacaatcat attcatgtgt cccatttctg gatgtaataa
    11341 cccatgaact aaatttcctt caacaatagc accacatcca attcctgtac caattgtcat
    11401 atacaaagca ctgtctaaac ctttggctgc accaaaataa gcttcaccta aagcagcacc
    11461 attaacatca gtatcgaatc ccataggaat atcataacgt tcctttaaag cacccatgat
    11521 attaaagtta ccccatcctg gttttggtgt tgttgtaata tatccataag ttggactgtt
    11581 taaatctgga tcgattggtc caaaacatcc gactccaagt gcctcaatat ctttt
//
LOCUS       DAOMTA010000009        11364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_28_length_11364_cov_2.423026, whole genome shotgun sequence.
ACCESSION   DAOMTA010000009 DAOMTA010000000
VERSION     DAOMTA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28_length_11364_cov_2.423026"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLK68_00605"
     CDS             complement(<1..555)
                     /locus_tag="PLK68_00605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRM88939.1"
                     /translation="MIDISIVVPMYNVENYIEKCLESLLKQATDIAYEIILVDDGSSD
                     NTVRICKKYKNKYKNISILSKKNGGTSSAKNIGIENSKGKYVVFVDSDDYVSENYISN
                     LVLDNDDIDFVVGGYSIKYNDGSIINNLPLSSVKSSRNLEELLINIGCDGLLNVDVGK
                     LYRLEILNNYKIRFNENMSTGEDLV"
     assembly_gap    593..606
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<607..>1163)
                     /locus_tag="PLK68_00610"
     CDS             complement(<607..>1163)
                     /locus_tag="PLK68_00610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRM88940.1"
                     /translation="DIIQRPLSIEEKKELKKELGIKDTFTLIAVGKIIPIKGFDLLLN
                     LMNDINFDLQLLIIGANDNESVNEYKKNSNIKVINFLSKEELNQYYLASDVFILPTRG
                     DVWGLVIGEAMAKGLPVLTTNKCLAGMAMVKEDKNGYIFNVDDLQDMKKSIMKLYNSD
                     LFEFGNESIEMAKKYCIENSAHYDY"
     assembly_gap    1164..1173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1174..1648)
                     /locus_tag="PLK68_00615"
     CDS             complement(<1174..1648)
                     /locus_tag="PLK68_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88941.1"
                     /translation="MKILFVTQNEAPFRMKWLDELAKYAKIIIFHLNDYEDNVNKKYI
                     SYHTKIAKTEDISRMVLNKKVYDYKKIFKTDYDILLLDGYGFFSQQLLILLLNIKKKK
                     YGISVDGGFIPKNENILKKSFKRYLLKRASFYFSTSKETDEFLEYYGVNINNIYRH"
     gene            complement(1664..2611)
                     /locus_tag="PLK68_00620"
     CDS             complement(1664..2611)
                     /locus_tag="PLK68_00620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM88942.1"
                     /translation="MISVVMATYNGEKYILEQLDSIRNQSISIDEVIICDDCSIDNTV
                     NIIKQYINDYSLNNSWRIIQNVENKGYIHNFQYALSVSIGDIIFLSDQDDIFYPNKFE
                     HMMNYLSMEDCLLLNANYEMIDDKSLVLRGKRFNSPKRKSKICKLDFKKFIYNSNYPG
                     FSIGIKHEIVNDFSKINLKNCYGHDIFLNVLALKKNGCYETREILSQYRIHNSNTSNI
                     GNDMTNLKIEKRLIQKNKELQEYKKLLEFLKELDFPISDIVFLTKRMNCLNKRIRYIK
                     NKNIFKLLYLMLTTKYYPKKTILGDVYYIIVNNYDSKKD"
     gene            complement(2608..3570)
                     /locus_tag="PLK68_00625"
     CDS             complement(2608..3570)
                     /locus_tag="PLK68_00625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRM88943.1"
                     /translation="MKIGVVVPVYNVEKYIERSIKSICDQNFKDFEVILVDDETQDNS
                     IEIASKLLDENNIKYKIIHQKNKGLPCARNTGIKECESEYICFIDSDDCITKNHLFNL
                     LTALNLKNDIYVAHSDYEIVADNNYMGNSESNMETTVISQQNLLDSFSKRNPAIHCCS
                     LLIDKKFLIDNNLFFNEKLKYGEDVEFMWRLFSKINNIGISFEKSYKYLINDQGIMKT
                     ISMEKGLVFCKEFSNTMGILSDTCPENKIIYMRAYYRNILGFFHVFSTYNNINDVKVI
                     YDELPYKLFFKYLKFSGLKIAILSFLLQNNILLFHKVFHKGDTK"
     gene            complement(3572..>3709)
                     /locus_tag="PLK68_00630"
     CDS             complement(3572..>3709)
                     /locus_tag="PLK68_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRM88944.1"
                     /translation="AIDMLNNNDNGIPVPNYTDEIVSTKVVKIIQSYTGVVDKMVWRK
                     L"
     assembly_gap    3710..3719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3720..4757)
                     /locus_tag="PLK68_00635"
     CDS             complement(<3720..4757)
                     /locus_tag="PLK68_00635"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HRM88945.1"
                     /translation="MEIKTDYSDVKFQDNGKLKLLIIVGTRPEIIRLAATINKCRQYF
                     DCVLAHTGQNYDYNLNGVFFHDLKLKDPEVYMDAVGDDLGATVGNIINCSYKLMNQIK
                     PDALLILGDTNSCLSAISAKRLHIPIFHMEAGNRCKDECLPEETNRRIVDIISDVNLA
                     YSEHARRYLAECGLPKERTYVTGSPMAEVLHSNFEDIKASDILEKLNLKPKEYMLLSA
                     HREENIDTEKNFLSLFTAINKLAEKYDMPILYSCHPRSRKRLESTGFKLDSRVFMHEP
                     LGFHDYNNLQMNAFCVVSDSGTLPEESSFFTSVGSPFPAVCIRTSTERPEALDKACFI
                     LAGIDEKSLLQS"
     gene            complement(4763..5959)
                     /locus_tag="PLK68_00640"
     CDS             complement(4763..5959)
                     /locus_tag="PLK68_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HRM88946.1"
                     /translation="MKVLVTGSNGFVGKNLVASLKNIRDGKDRTRSFFVEEVYEYDID
                     TAPSLLDTYCKDCDFVFNLAGVNRPKDNGEFMQGNFGFASTLLDTLKKHNNTCPIMLS
                     SSIQATLIGRYGTSDYGKSKLAGEELFFDYSKETGSKVLVYRLPNLFGKWCRPNYNSA
                     VATFCNNIANDLDIQVNDRSTELELLYIDDLVEEMLSALTNDEHHCNYDGLNIVDNNT
                     GKYCYVPTTHKATLGEIVDLLESFKNQPNTLVMPEIPNNSFAKKLYSTYLSYLSKEKA
                     IFDLKMNKDDRGSFTELLKTENCGQFSVNISKPGITKGQHWHHSKWEFFIVVSGKALI
                     QERRIDSNEVIEFEVSGDNIQAIHMLPGYTHNIINLSDTQDLVTVMWANEAFDPNHPD
                     TFFEEV"
     gene            complement(5959..7005)
                     /locus_tag="PLK68_00645"
     CDS             complement(5959..7005)
                     /locus_tag="PLK68_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRM88947.1"
                     /translation="MSKFMGKTLLITGGTGSFGNAVLNRFLNTDIKEIRVFSRDELKQ
                     DNMRHEYQAKYPDVFNKLKFYIGDVRDIQSIKNAMHGVDYVYHAAALKQVPSCEFFPI
                     EAVKTNVLGTENVLTAAIEAGVKRVVCLSTDKAAYPVNAMGTSKAMMEKVIVAKSRTV
                     DPEKTSIMCTRYGNVLASRGSVVPLFINQIKAGNDLTVTSAEMTRFVMTLEEAVDLVL
                     FAFENGQSGDIFVQKAPACTIGVLTDAVRRLMKADNKVKVIGIRHGEKMYETLLTDEE
                     CANAVDLGDFYRVPADKRDLNYDKYFNEGIEESTLTQFNSNNTHLMDVESVMNKLMEV
                     PLVQEEVAAWENEK"
     gene            complement(7002..8243)
                     /locus_tag="PLK68_00650"
     CDS             complement(7002..8243)
                     /locus_tag="PLK68_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRM88948.1"
                     /translation="MNDVEKKKHILVVSQYFYPEQFRINDICTEWVKRSYKVTVLTGI
                     PNYPQGKYYEGYGMNSKREEEWNGIRIIRIPLIPRGNSAVGMVANYMSFVGSGFIWQS
                     LTNIEADFVFIFEVSPMTQALVGVWYAKKKKIPCYLYVQDLWPENVEIVTGITNPAII
                     KPVDKMVKYIYKNCDHVFATSPSFVKEIQKKCDDKEKVSYWPQYAEDFYKPIDQKVAK
                     KNVPEIPNDDSFKIIFTGNIGKAQGLEILPKIAKELKNENIKFIIVGDGREKENFIQS
                     IKDNDVEDMFIMIDKQPATRIPEMLSCCDSAFLSFMDNDLFNKTIPAKLQSYMACGMP
                     IVAVAGGESKRIINEAKCGVVIPIGEDKKFVAKIQELKSTDKSKLKEFGNNSYRYCND
                     NFNQVDLMNYIDNYFKKEKLL"
     gene            complement(8247..>9983)
                     /locus_tag="PLK68_00655"
     CDS             complement(8247..>9983)
                     /locus_tag="PLK68_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRM88949.1"
                     /translation="VLSGGAIVVLSPVLGLLALAIKLDSKGPVLFKQKRVGKDKELFE
                     IYKFRTMRTDTPSDMPTHMLKDPNQFITKTGKLLRKTSLDELPQIFNIFTGKMSIIGP
                     RPALWNQDDLIAERDKYHANDVTPGLTGWAQINGRDELEIDVKAKFDGDYVNEMGLKM
                     DIKCFLATIGSVLLGDGVVEGGTGELEETVDTQIVDPEKIDKEAKIGAAVVGTAGVVG
                     LTGLGLTYKYLKSKDNKKDNKSHKGLLLSSLALGYVTYTTYINVKRKVQLQDNFVNEE
                     ELKQTNLDKHLPLKKVLITGANSYIGESVEKWLNNSDNEYEVDTLDMLDPNWKEFDFS
                     KYDTVFHVAGIAHTDVGNVSEEVKQKYYQVNTDLTLEVANIAKNAKVRQFIFMSSMIV
                     YSGCGTTHITKDTKPKAENFYGDSKLQADLKLQEMNDDSFKVVVVRPPMIYGKGSKGN
                     YPQLVKLATKLPVFPIVNNKRSMLHIDNLCEFIKLMIDNEESGVFFPQNSKYTNTSDM
                     VQMIASVKGHRIIMLPGTSLSIKLLENVPGKIGGLAKKAFGDSYYDMSLSEYKDEYRV
                     NGLRNSIEKTEG"
     assembly_gap    9984..9993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10081..10641)
                     /locus_tag="PLK68_00660"
     CDS             complement(10081..10641)
                     /locus_tag="PLK68_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid O-acetyltransferase"
                     /protein_id="HRM88950.1"
                     /translation="MNLLIIGAGGHGRCCLDIARDMNIFDKISFLDDQNINEVINDCK
                     IIGSIDEMSSYYPEYTHIHIAIGNNSVRSKLLLQAKEIGYSLPILQHSSSVVSNYASI
                     NEGTIIFPHAVIEPNATIGQGCIITANTTINHDAMINDGCLIYSNSIIRPMSVIGSNT
                     RIGSGCTITFGTDIKEETDIKDGSII"
     gene            complement(10653..10892)
                     /locus_tag="PLK68_00665"
     CDS             complement(10653..10892)
                     /locus_tag="PLK68_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88951.1"
                     /translation="MTKILEKIESEVICIIDDKQYQYTNGKEAYQQLTNNYSITSIKA
                     FNNQIILNLNPKENNKEQDWQEEYKKQFGEEPSFF"
     gene            complement(11043..>11364)
                     /locus_tag="PLK68_00670"
     CDS             complement(11043..>11364)
                     /locus_tag="PLK68_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88952.1"
                     /translation="KQNDFNDLLLSMKDKNDGLIKQLENAEVSALREKEIEFFNNILD
                     KYNVARVSVGYQEEGKTIITEGPLLHYQDHIVRVMKHHCTAQLDLPFFDRKIILGVEL
                     ISKN"
BASE COUNT         3620 a         1965 c         1264 g         4471 t
ORIGIN      
        1 aactaaatcc tctccagtag acatgttttc attgaaacga attttataat tattcaaaat
       61 ctctaatcga tataattttc cgacatccac atttaataat ccatcacaac ctatgtttat
      121 tagtaactct tccaaattac ggcttgattt gactgagctt aagggtaaat tattaatgat
      181 gctaccgtca ttgtacttaa tactatatcc accaacaaca aaatcaatat catcattatc
      241 taatactaga tttgatatat agttttcact tacataatca tcactatcta caaatacaac
      301 atattttcct tttgaattct ctatgccgat gttttttgct gatgatgtcc caccattttt
      361 tttgcttaaa attgaaatat ttttatattt atttttatat tttttgcaaa ttcttactgt
      421 gttatcacta cttccatcat ctactagaat tatctcatat gcaatatctg tagcttgttt
      481 cagtaaagat tctaaacact tttctatgta attttcaaca ttatacatag gtacaacaat
      541 cgaaatatct atcatttgat taatttcctt tcgtttattc ttttgaaatt acnnnnnnnn
      601 nnnnnngtaa tcataatgag ctgaattctc tatacaatac ttttttgcca tttctatact
      661 ctcattacca aattcaaaca agtctgaatt gtataatttc ataatacttt ttttcatatc
      721 ttgtaggtca tcaacattaa atatatatcc atttttatct tcttttacca ttgccatacc
      781 agctagacat ttatttgtcg tcaaaactgg caacccttta gccatagctt ctccaataac
      841 tagcccccaa acatcaccac gagtaggaag aataaataca tcgcttgcca agtaatattg
      901 atttaattct tcttttgata aaaaattgat tacttttata tttgagtttt ttttatattc
      961 atttactgat tcattgtcat ttgcaccaat gattaataat tgtaaatcga aattaatgtc
     1021 attcattaga tttaataata aatcaaatcc ttttattgga attatttttc ctacagcaat
     1081 tagtgtgaag gtatccttta tacctaattc ttttttaagc tctttttttt cttcgataga
     1141 aagcggtctt tgaataatat catnnnnnnn nnnaatgtct ataaatatta ttgatattca
     1201 ctccataata ttctaaaaac tcatcagttt ctttagacgt gctaaaataa aaacttgctc
     1261 tttttaatag atatctttta aaactttttt taagtatatt ttcatttttt ggtataaacc
     1321 caccgtctac agatattcca tatttttttt tctttatatt tagtagcaaa attaataact
     1381 gctgtgaaaa aaaaccatat ccatctaata ataaaatatc ataatcagtt ttaaaaattt
     1441 ttttataatc atatactttt ttatttaaaa ccattcttga tatgtcttct gttttagcta
     1501 ttttagtatg atatgaaata tattttttat ttacattatc ttcataatca ttcaaatgaa
     1561 aaataattat ttttgcatat ttagctaatt catctaacca tttcattcta aatggagctt
     1621 cattttgcgt aacaaatagt attttcacgt acattctcct attctaatcc ttttttgaat
     1681 cataattgtt aacaataata taataaacgt ctccaaggat tgtttttttt ggataatatt
     1741 ttgttgtaag catcaagtac agcaatttaa atatattttt attttttatg taccgtattc
     1801 ttttatttag acaattcatt ctctttgtta aaaaaacaat atcacttatg ggaaaatcta
     1861 attcttttaa aaattctaaa agtttcttgt attcctgtaa ttctttattt ttttggatta
     1921 atcgcttttc tatttttaaa ttggtcatat catttccaat attacttgta ttgctattat
     1981 gtattctgta ttgactcaat atttctcttg tctcataaca gccattcttt tttaacgcaa
     2041 gaacattcaa aaaaatatca tgcccatagc agttttttaa atttattttt gaaaaatcat
     2101 ttacaatttc atgctttatt ccaatagaaa atccaggata attggaatta tatataaatt
     2161 ttttaaaatc cagtttacaa atttttgatt ttctttttgg actattaaac cttttacctc
     2221 ttaaaaccaa tgatttatca tctatcattt cgtaatttgc atttaataat aagcaatctt
     2281 ccattgaaag gtaattcatc atgtgctcaa atttattagg ataaaaaata tcatcttgat
     2341 ccgacaaaaa aattatatct ccaatactta ctgatagtgc atattgaaaa ttgtgtatgt
     2401 atcctttatt ttctacattt tgaatgatac gccagctatt attcaaacta taatcattta
     2461 tatattgttt tatgatatta acagtattat ctatagaaca gtcatcacaa ataattactt
     2521 cgtctatgct aattgattga ttcctaattg aatctaattg ttctaaaata tacttttctc
     2581 cattataagt agccatcaca acagaaatca ttttgtgtct cctttatgaa atactttatg
     2641 aaataataaa atattatttt gtagtaaaaa cgataatatt gcaattttta aaccagaaaa
     2701 ctttaaatac ttaaaaaata atttatatgg cagttcatca taaataacct taacatcatt
     2761 aatattatta taagtggaaa acacatggaa aaaccctaga atatttctat agtatgctct
     2821 catatatatt attttatttt ctggacatgt atccgacaaa atccccatag tattactaaa
     2881 ctctttacaa aaaactaaac ctttttccat tgatattgtt ttcataatac cttgatcatt
     2941 tattagatat ttataggatt tttcaaatga aattccaata ttattaattt ttgaaaataa
     3001 tctccacata aattcgacat cttctccata ttttaacttt tcattaaaaa acaaattatt
     3061 atctattaaa aattttttat caattaatag agaacaacaa tgaatagctg gatttcgttt
     3121 gctaaaagaa tcaagcaaat tttgttgact aataacagtc gtttccatat ttgattcgct
     3181 attgcccata taattattat cagcaacaat ttcgtaatca ctgtgtgcca catatatatc
     3241 gttttttaag tttaatgctg ttagtaaatt aaataaatga tttttagtga tacaatcatc
     3301 cgaatcaata aaacagatat attcactctc acattctttt atgcccgtat ttcttgcaca
     3361 aggcaagcct ttattctttt gatgtattat tttatattta atattatttt catctaataa
     3421 ttttgatgca atttctattg aattatcctg ggtctcatca tcaactaata ttacttcaaa
     3481 atctttaaag ttctgatcac atatggattt tatagatcgt tctatatatt tctcaacatt
     3541 ataaactgga acaactacac caatcttcat actataattt cctccaaacc atcttatcaa
     3601 ctacaccagt ataagattgg ataatcttaa ctactttagt agatacaatt tcatcagtat
     3661 agtttggtac tggaatacca ttatcattat tatttaacat atctatagcn nnnnnnnnnt
     3721 gattgcaata aagatttttc atcaatacct gctaagataa aacaagcttt atctaatgct
     3781 tctggtcttt ctgtagatgt tctaatacat actgcaggga atggactccc tactgatgta
     3841 aaaaatgacg attcttctgg aagtgttcca ctatcagata ctacacaaaa tgcattcatt
     3901 tgaagattat tataatcatg gaatcctaat ggttcgtgca taaatactct agaatctaat
     3961 ttgaatcctg tggattctaa tctttttcta gatctaggat gacacgaata tagaataggc
     4021 atatcatatt tttctgctag tttattaata gcggtaaata gtgataagaa gttcttttct
     4081 gtatcaatat tttcttctct atgggcactc aataacatat attcttttgg tttaaggttt
     4141 agtttttcta aaatgtcact agctttaata tcttcaaagt tactatgtaa tacttctgcc
     4201 ataggtgaac ctgttacata tgttctttct ttaggaagac cgcattctgc tagatatctt
     4261 cttgcatgtt cactatacgc taagtttaca tcagaaataa tatctacgat tcttctatta
     4321 gtttcttcag gtaagcattc atctttacat ctattacctg cttccatatg gaagatagga
     4381 atatgtaatc ttttagcact aatagcactt aagcaactat tagtatctcc taaaattaat
     4441 aatgcatcag gtttgatttg attcattagt ttatatgaac agttaatgat attaccaact
     4501 gtagcaccta aatcatcacc tactgcatcc atatatactt ctggatcttt taattttaag
     4561 tcatggaaaa atactccatt taagttgtag tcatagtttt gaccagtgtg tgctaacaca
     4621 caatcaaaat attgtctaca tttattgatt gttgcggcaa gacgaatgat ttcaggtctt
     4681 gtacctacaa tgatcaataa tttaagtttt ccattatctt ggaactttac atcactataa
     4741 tctgttttaa tttccattct acttatacct cctcgaagaa tgtatctgga tgatttggat
     4801 caaaggcttc attagcccac ataacagtaa ctaaatcttg ggtatctgat agattaataa
     4861 tgttatgagt atatcctggt aacatatgaa tagcttgaat gttatctcct gatacttcaa
     4921 actctattac ttcattacta tctattcttc tttcttgtat tagagcttta cctgatacaa
     4981 caataaagaa ttcccattta ctatgatgcc aatgttgtcc tttagtaatt cctggttttg
     5041 atatattaac agagaactgt ccgcagtttt ccgtttttaa taattcagta aaacttcctc
     5101 tatcatcttt attcattttt aaatcaaaga tagctttttc tttagataaa tatgacagat
     5161 aagttgaata tagtttttta gcaaaactat tatttggtat ttctggcatt actaatgtat
     5221 taggttgatt tttaaaagat tctaataaat caacaatctc acctaatgta gctttatgtg
     5281 ttgttggaac ataacaatac ttaccagtat tgttatcaac aatatttaaa ccatcatagt
     5341 tacaatgatg ttcatcatta gttaatgcac ttagcatttc ttcaactaaa tcatcaatat
     5401 ataataactc taattcagta cttctatcat taacttgaat atctaaatca ttagcaatgt
     5461 tgttacaaaa tgtagcaaca gcactattgt aattaggtct acaccattta ccaaatagat
     5521 tagggagtct ataaactaat actttagaac cagtttcttt agaataatca aagaataatt
     5581 cttctccagc cagtttactt tttccgtaat cacttgttcc atatctacct attaaagtag
     5641 cttgaattga tgaagataac ataataggac aagtattatt atgtttcttc aatgtatcta
     5701 ataatgtaga tgcaaagcca aagttacctt gcataaattc accattatct ttaggtctgt
     5761 ttactcctgc aagattgaag acaaagtcac aatctttgca gtatgtatct aataatgatg
     5821 gagcagtatc aatatcatat tcatagactt cttcaacaaa aaaagatctc gtacgatcct
     5881 ttccatctct tatattcttt aatgaagcca ctaggttttt accaacaaaa ccatttgatc
     5941 ctgtaactag tactttcatt atttttcatt ctcccatgca gcaacttctt cttgtactaa
     6001 tggtacttcc attagtttat tcataactga ttctacatcc attaaatgtg tattattaga
     6061 gttgaattga gtaagtgtag attcttcaat accttcattg aagtacttat cataatttag
     6121 atcacgttta tctgcaggta ctctatagaa gtcacctaaa tccactgcat tagcacattc
     6181 ttcatcagta agtaatgttt catacatctt ttcaccatgt ctgataccaa taactttaac
     6241 tttattatca gctttcatta atcttctaac agcgtcagtt agtacaccaa ttgtacaagc
     6301 aggagctttt tgtacaaaga tatctcctga ttgaccattt tcaaaagcaa ataatactaa
     6361 gtctactgct tcttctaatg tcatgacaaa tcttgtcatt tctgcactag taactgttaa
     6421 gtcatttcct gctttaattt ggttaataaa aagaggtacc actgaacctc ttgaagccaa
     6481 tacattacca tatcttgtac acataatact tgttttttct ggatctactg ttctagattt
     6541 agctacaatt actttttcca tcatagcttt actagtaccc atagcattta ctggataagc
     6601 agctttatct gttgataaac atactactct tttaactcct gcttcaatag cagctgttaa
     6661 cacattttca gtacctaata cattagtttt aactgcttct attggaaaga attcacaact
     6721 aggtacttgt tttaatgcgg ctgcatgata gacatagtct acaccatgca tagcattctt
     6781 aatactttgt atatctctta catcacctat ataaaacttt aacttattaa aaacatcagg
     6841 atatttagcc tgatattcat gtctcatatt atcttgtttt aattcatctc tactaaatac
     6901 tcttatctct tttatatctg tatttaaaaa gcggtttagc accgcatttc caaaactacc
     6961 tgtaccacct gtaattaata gtgtttttcc cataaattta ctcataatag tttctccttt
     7021 ttaaaataat tatctatata attcattaaa tctacttgat taaaattatc attacaatat
     7081 ctatagctat tattaccaaa ttctttcaac ttagatttat ctgttgattt taattcttgt
     7141 attttagcaa caaatttctt gtcctctcca ataggaatta ctacaccgca tttagcttca
     7201 ttgattattc ttttggactc tccaccagca acagcaacta taggcatccc acatgccata
     7261 tatgattgta gttttgctgg tatagtttta ttaaataaat cattatccat gaaagataaa
     7321 aatgccgaat cgcaacatga taacatttca ggaattctag tagctggttg tttgtcaatc
     7381 ataataaaca tatcttcaac atcattatct ttaattgatt gaataaagtt ttccttttct
     7441 cttccatctc caacaataat aaatttaata ttctcatttt ttaattcttt tgcaatctta
     7501 ggtaatattt ccaacccttg tgcctttcca atgttaccag taaatattat tttaaaacta
     7561 tcatcattag gtatctcagg aacattcttt tttgctactt tttgatcaat aggtttataa
     7621 aaatcttctg catattgtgg ccaataagat actttttctt tatcatcaca ttttttttgt
     7681 atttctttta caaatgatgg tgatgtagca aaaacatgat cacaattttt ataaatatac
     7741 ttaaccatct tatcaacagg ttttattata gctggatttg taatccccgt tactatttca
     7801 acattttcag gccataagtc ctgaacatat aagtaacatg gtattttctt tttcttggca
     7861 taccatactc caactagtgc ctgagtcatt ggtgatacct caaatataaa tacaaaatca
     7921 gcttcaatat tagttagtga ttgccaaata aacccacttc ctacaaatga catataatta
     7981 gccaccatac caactgcaga attacctcta ggaataagtg gtattcttat aattctaata
     8041 ccattccatt cttcctctct cttactattc ataccgtatc cttcataata ttttccttga
     8101 ggataattgg gaatacctgt taatacagtt actttataac ttcttttcac ccactctgta
     8161 caaatatcat ttatacgaaa ttgttcagga taaaaatatt gagaaacaac aaggatatgt
     8221 ttctttttct ctacatcatt catatgttac ccctctgttt tctcaattga gttacgtaat
     8281 ccattaactc tatattcatc tttatattca cttaaactca tatcataata tgaatcacca
     8341 aatgcttttt tagctaatcc accaatttta cctggtacgt tttctaataa ttttatagat
     8401 aatgaagttc ctggtaacat aataattctg tgtcctttta cactagctat catttgtacc
     8461 atatctgatg tatttgtata tttagaattt tgcgggaaga acacacctga ttcttcatta
     8521 tcaatcatta acttgataaa ttcacaaaga ttatctatat gtagcattga tcttttatta
     8581 ttgacaattg gaaatactgg caattttgtt gcaagtttta ctaattgcgg ataattacct
     8641 tttgatcctt ttccataaat cattggtggt cttactacca caaccttgaa tgaatcatca
     8701 ttcatttctt gtagttttaa atcagcttgt aatttactat caccatagaa gttttctgct
     8761 ttaggtttag tatcttttgt aatatgagtg gttccacaac cactatatac aatcattgaa
     8821 gacataaaga taaattgtct tacttttgca ttttttgcga tgtttgctac ttctaaagtt
     8881 aagtcagtat ttacttgata atatttttgt tttacttctt cagaaacgtt tcctacatca
     8941 gtatgagcga ttccagcaac atggaatact gtatcgtatt tagaaaagtc aaattctttc
     9001 caattaggat ccaacatatc taaagtatca acttcatatt cgttatcaga attgtttaac
     9061 catttttcta ccgattcacc aatataactg ttagcaccag taatcaagac tttttttaga
     9121 ggaagatgtt tatctaaatt agtttgttta agttcttctt cattaacaaa gttatcttgt
     9181 aattgcactt ttctttttac attaatgtac gtagtatatg ttacataacc taatgctaat
     9241 gagcttagta acaatccttt atgagattta ttatcttttt tattgtcttt gctttttaaa
     9301 tatttatatg ttagtcctaa gccagtaagt ccaactactc ctgcagttcc tactactgca
     9361 gcacctattt tagcttcttt atcaatcttt tcaggatcta ctatttgtgt atcaacagtt
     9421 tcttctaatt caccagttcc accttctact acaccatctc ctaatagtac tgaaccaata
     9481 gttgctaaga aacatttgat atccatcttt aagcccattt cattaacata atcaccatca
     9541 aacttagctt tcacatctat ttctaattca tctctaccat taatttgtgc ccagccagtt
     9601 aatcctggag ttacatcatt cgcatgatat ttatctctct ctgcaatcaa gtcatcttga
     9661 ttccataaag ctggtctagg acctatgatg gacatctttc ctgtaaagat attaaagatt
     9721 tgtggtaatt catctaaaga tgtttttctt aataattttc ctgttttagt aataaattga
     9781 tttggatctt ttaacatatg agtaggcata tcactaggtg tgtctgttct catagttcta
     9841 aacttataga tttcaaatag ttctttatct ttgcccacac gtttttgttt aaagagtact
     9901 ggtcctttag aatctagttt tatagctaac gctaataatc ctaatactgg acttagtaca
     9961 acaatagctc caccacttaa aacnnnnnnn nnntctattg ttcttttaag gggcagatat
    10021 tttttttgtt tatcagtaat catattttcc ctccctgttt tatttcaatt aaaaagatga
    10081 tcaaattatt gagccatctt taatatcagt ttcttctttt atgtctgtac caaaagtaat
    10141 cgtacatcca cttcctattc ttgtgttact tcctatgact gacattggtc taattataga
    10201 attgctatat attagacagc catcattgat cattgcatca tgattaatag ttgtatttgc
    10261 agtaataata catccctgac caatagttgc attaggttct attactgcat gaggaaatat
    10321 aattgttccc tcattaatag aagcataatt tgatacaaca cttgaagagt gttgtagtat
    10381 aggcaatgaa tagcctattt ctttagcttg taaaagcagt ttgcttctaa cactgttatt
    10441 gccaatagca atatgaatat gagtatactc gggataataa gaactcattt catcaataga
    10501 accaatgatt ttacaatcat ttataacttc attaatattt tgatcatcaa gaaaagatat
    10561 cttatcaaag atattcatat ctctagctat atctagacaa catctcccat gtccacctgc
    10621 tcctatgatt aatagattca ttttcactcc ccctaaaaaa agcttggttc ttcaccaaat
    10681 tgtttcttat attcttcttg ccaatcttgt tctttgttat tctcttttgg atttaagttt
    10741 aagatgattt gattgttaaa agctttaata gatgtaatag aatagttgtt tgttaattgc
    10801 tgataagcct ctttaccatt tgtatattgg tattgtttat catcaataat acatatcact
    10861 tctgattcta ttttttctaa tattttagtc actctttata cctccttttt gaacataaaa
    10921 aaacaggttc atcgcctgtt ttagtcatat ttatgaggta gaagatacaa ttccccctta
    10981 ccccaaataa tctagacttt atgctcagaa ctaatgtgtt atcaagttaa cctgagtaac
    11041 acttaattct tgcttataag ttcaactcct agaataatct ttctatcaaa gaaaggtaaa
    11101 tctagttgtg ctgtacaatg atgtttcatg actctgacaa tatgatcttg ataatgcagt
    11161 aggggacctt ctgttataat cgtcttaccc tcttcttggt aaccaacaga tactctagca
    11221 acattatatt tatctaatat attattaaag aactctattt ctttttctct taaagcagat
    11281 acttcagcat tctctaattg tttaatcagt ccatcattct tatctttcat agacaataaa
    11341 agatcattaa aatcattttg tttt
//
LOCUS       DAOMTA010000010        10571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_37_length_10571_cov_2.320369, whole genome shotgun sequence.
ACCESSION   DAOMTA010000010 DAOMTA010000000
VERSION     DAOMTA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10571
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_10571_cov_2.320369"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLK68_00675"
     CDS             complement(<1..198)
                     /locus_tag="PLK68_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM88953.1"
                     /translation="MESKFKIIIMDVDGTLTNSQKIVTPKTKAALMKAQELGIKLILA
                     SGRPTSGLIGLGHELQMDKYHG"
     gene            complement(319..1233)
                     /locus_tag="PLK68_00680"
     CDS             complement(319..1233)
                     /locus_tag="PLK68_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88954.1"
                     /translation="MKKFNYYFTLLLVAFITLFSFFNMFQIVSLSNNVIKIIINNLLP
                     SLLPFMILISLCLSLGLLNLFSYLIQWIFKPLFKLSPIMSSIYFISFFCGYPTNVKMI
                     KEAYELNYINSQELQHLLSIASFASISFIFVSLHLEYPLIIYFCHLAPSIIKALFYHQ
                     HYEFQSLKESIIRLKKPHVSFVEALKQSILSSCYAFIFILGYMLVFQFVGYAIGHIIK
                     DSFINAIIQGVLEFSSGSLQLLQFSHTPLVYSLICFNLSFSGLSVMMQTDNLLDGIDY
                     SFKAYFKARLFHGLCSFTLCLLILTYLL"
     gene            1288..2517
                     /gene="hflX"
                     /locus_tag="PLK68_00685"
     CDS             1288..2517
                     /gene="hflX"
                     /locus_tag="PLK68_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRM88955.1"
                     /translation="MMKALIVGVRYKEMNYDLDNSLIELEELCKACNIEVIKRCVQNL
                     DKINPSLYIGTGKVQEIKGQLDNLDLVIFNEELSPLQVKNLTDILDIEVTDRTALILR
                     IFEVRAKTKEAKLQVEIAKGQYLLPRLAGMKEHLYSQQGGSGFRGSGEKQIELDRRLV
                     SNQIFNAKKQLANIVKQRQNQRKRRKDNEMKVIALVGYTNSGKSTLMNAFCLNKNKQV
                     LQKDMLFATLETATRHITINQHPCLLTDTVGFIERLPHHLIQAFRSTLEEVVEADLLI
                     HVVDASNPNYEQYINTTNAVLKELGIKDTPMIYAYNKVDLNKYGFIVPLEPYAFISAK
                     ERIGLEVLEKSISDILFKDYAIYDLNIPYQDGEVFKYLHQHCLVLEFQYLENSIYMKI
                     EAHPRFMVQYDQYLLKH"
     gene            2531..2722
                     /locus_tag="PLK68_00690"
     CDS             2531..2722
                     /locus_tag="PLK68_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88956.1"
                     /translation="MDNEKKLFRLDLSIAVEATSAQEAFDILVTDETLKQIRELVIKS
                     KDNIKEMFEKEDSEPAIIN"
     gene            2782..3768
                     /gene="argF"
                     /locus_tag="PLK68_00695"
     CDS             2782..3768
                     /gene="argF"
                     /locus_tag="PLK68_00695"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRM88957.1"
                     /translation="MDLYGRNFLTLKDFSKEEIRYFLDLAHQLKKDKKEGKTSNSLAG
                     KNIVLLFEKTSTRTRCAFEVAALDEGAHVTFLSNSQMGKKESIEDTAKVLGRMYDGIE
                     FRGFEQSTVEALAKYSGIPVWNGLTDVDHPTQTLADFLTIEEHLDKPLNEVKFVFTGD
                     IRNNVCYGLMYGAAKMGMHFVALGPSELQMDPEVVAYCKAEAEKSGGSFMVTDNVDEA
                     VRDADVIYTDIWVSMGEAEELYPQRVKLLSPYKVTQAMMAKTGNDKTLFMHCLPSFHD
                     FETTLAKEKYEQGIDIREVEDEVFRSANSVVFDEAENRMHTIKAVMVATLGK"
     gene            complement(3777..4421)
                     /locus_tag="PLK68_00700"
     CDS             complement(3777..4421)
                     /locus_tag="PLK68_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88958.1"
                     /translation="MVDEFYLDVNLELFVQWLFGNKNKYDHNILCTLKSNEPYGKTIT
                     FNYYNISGSIVIWANGIIEEQIFDDEENLLFYLHYKLVSLAQGQKLFYDFYDSLTKHT
                     KCPPLKIILCCSGGLSSSFFANKLAELISLKHLNYEIIPLGFYQLNSSYLDCDAIYLA
                     PQISYLEPQAMNIVKNTVPVHCVTPSVYATYNYRGLLDMITNENISKTKENGTI"
     gene            4525..5307
                     /locus_tag="PLK68_00705"
     CDS             4525..5307
                     /locus_tag="PLK68_00705"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HRM88959.1"
                     /translation="MLKYDMIRLGDKMTGKTYVISDLHGHFDLLIKLLETISFSENDI
                     LYIIGDICDRGPDSLKILFYIQDHNNIILIKGNHEYMWQEALYYGVYYDDFDYPSRAL
                     KLWLANGGDTTMRNIREYLQKDKFRYEDYRVIRTAFFKSLYEYLINLPNYLEIEVNQQ
                     KYVLVHAGIDVRYPLKEQKLNSLLWIRDDFYHARGDLTKIYIFGHTPTAMLNKDRSFD
                     IWVDDVHQNKIGIDGGLACGVIGQLNCLCLDDGKIIIVNKEE"
     gene            5311..6135
                     /locus_tag="PLK68_00710"
     CDS             5311..6135
                     /locus_tag="PLK68_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM88960.1"
                     /translation="MRGAFYNLVNFINTTSINDVYANAARKILQNIYVIPGSTITDVA
                     ELCFVSTATISRLCRKLNYESFADFKMDITMNLNYFNRDTLRLQFDHQLPQRQYLHKG
                     KEVFKAHFDNILDNLKETYESVSYEDLEFIVDKIHDANKVCFAGNFFTQSVSMQLQIE
                     LSYLGKECSGMYPLEQQILTVEGLDENDVIIVSSIAGGFMTDHPDVMRVISKSPAYKI
                     VISQLDEFVYSDSIDMILKVGTDHHSLIGKFSITYIFEVLEALYHLKYGVKEKREL"
     gene            complement(6160..6888)
                     /locus_tag="PLK68_00715"
     CDS             complement(6160..6888)
                     /locus_tag="PLK68_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HRM88961.1"
                     /translation="MKLKKLKKIPAFIAFIIIFIFGYLCLQIIPPLLYNDNSGALDKV
                     DCPQYINEFVDKNPQAIELKENYQPNENTDPIILDSPNGIPLYIQWDKRWAYTTYGDE
                     IIGTAGCGPTCLSMVAVGLTGKTTYNPRYVAKYAIKNDFLEGSMTRWALMESGCNEFG
                     LIASAVPLSKNEMFKQLDAGHPIIASLRPGDFTTTGHFIVITKTINDKFLVNDPNSKE
                     NSHKQWSYSQLAPQIKAMWAYNTI"
     gene            complement(7001..7525)
                     /locus_tag="PLK68_00720"
     CDS             complement(7001..7525)
                     /locus_tag="PLK68_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM88962.1"
                     /translation="MEKKNNYLINITIALCSIYYFLMLIRIIILKNGFIDMGYNSNLV
                     LFDFINQYHQSGLTKVLLVNIIGNVSLFIPLSIILRHYFSFLNNYNIAFIGFFTSLSF
                     ELIQLGTGWGVFDIDDIFLNTLGCLIGIIIYHFINQHRQNNVSTSLFLLSFGSIGLIS
                     VYNYAPLLLTTFII"
     gene            7757..>7858
                     /locus_tag="PLK68_00725"
     CDS             7757..>7858
                     /locus_tag="PLK68_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88963.1"
                     /translation="MVREGIDVSQYQGQIDWELVKNHIDFAILRCGYG"
     assembly_gap    7859..7868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7869..8776
                     /locus_tag="PLK68_00730"
     CDS             <7869..8776
                     /locus_tag="PLK68_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRM88964.1"
                     /translation="IPGQDDPTFKRNADECTRLGIPFGVYLYSYATTESEALSEARHV
                     MRLVEGYKMEYPVYLDLEDPRIGQLSNEQIERNSRVWAEELVNNRYFPGFYASYYWWT
                     EKLTGPLFNRYTKWVARYAEELGAEGFDMWQYTDKGFVEGINAPVDRNYAYRDFPAEI
                     IAGGFNNFEGDETIPPITNYQVGDHVTFDHVFVSSDSTTPLIPYINHGTIRRVVKGAR
                     NPYLIGNGLGWVNNDSITGMMQYLSNPTYRGDSFVDALVQIGVDASFASRRELARKNG
                     IYDYTGSAKQNLELLRLLKEGRLRQ"
     gene            complement(8861..9958)
                     /locus_tag="PLK68_00735"
     CDS             complement(8861..9958)
                     /locus_tag="PLK68_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM88965.1"
                     /translation="MVILLNNTHFKQSLTANLLKKIGITISVTVVTFVILLLYSYEVY
                     HQYIWQEYDLVYQVAQIIKDNLVTISITLIAFDTIIIFWLRYRESLQYIEKMLEAGKT
                     LVADNNYLISLPYELKEIEDQMNQIKRESLQNKKAIKQAEQQRNDLLLYLAHDLKTPL
                     TSIVGYLDLLMNQPNLTIEEKSNYTKIAYDKAIRLEELIEEFFSIAKYNLSDISLERK
                     AVNLSMMLVQISYEFMPLYHEKNINCVTKIEDNLSLELDINQFERVFDNLIRNAINYS
                     KENSDLIITAKKEVDCIFIQVANFVKHISEHNLERIFQPFVRLDEARNSKTGGSGLGL
                     AITKKIIELHGGTIEADLTDDLITFTIKLPI"
     gene            complement(9939..>10571)
                     /gene="vanR"
                     /locus_tag="PLK68_00740"
     CDS             complement(9939..>10571)
                     /gene="vanR"
                     /locus_tag="PLK68_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HRM88966.1"
                     /translation="YLQNESYQVFKFYHGQEALDSLKKNHYDLAILDVMMPDLDGFSL
                     LKKIREDYFFPVIMLTAKNNDMDKINGLTLGADDYLTKPFNPLELAARVKTQLRRYTR
                     YNTNEPAVNQQIYDFNGLMINNDNHKCTLYDQPLNLTPIEFSILWYLCSKRGSVVTSE
                     ELFEAVWQEKYLDNNNTVMAHVARLREKMNENARKPKFVKTVWGVGYTIE"
BASE COUNT         3817 a         1384 c         1856 g         3504 t
ORIGIN      
        1 accatgatat ttatccattt gtaattcatg ccctaatcca ataaggccag atgttggacg
       61 cccagaagct aaaattaatt taatacctaa ctcttgtgct ttcattaaag ctgctttagt
      121 ttttggggta acaatttttt gactgttggt taatgttcca tcaacatcca taataattat
      181 tttaaatttg ctttccataa tcattctcct ctttacatct ttctattata ctgaaaatat
      241 ttgtcgagga cgattctttt taataaatga aaaaattatt tcattaaaaa taataagatg
      301 gttaaaaaaa tgataagctc ataacagata agttaaaatt aatagacata aggtaaatga
      361 acaaagacca tgaaataagc gagctttgaa ataagcctta aaactatagt caattccatc
      421 taaaagatta tccgtttgca tcattacact aagaccacta aaagatagat taaaacaaat
      481 taagctatat actagcgggg tatgactaaa ttgtaatagt tgaagacttc cagaactaaa
      541 ctcaagaact ccctgaataa tagcattaat gaagctgtcc ttaataatat gaccaatagc
      601 atatcctaca aattgaaaaa ccagcatata accaaggatg aaaataaaag cataacatga
      661 acttaaaatg ctttgtttca aagcttccac aaaactgaca tggggctttt ttagtctaat
      721 gattgattcc ttcaaacttt gaaactcata atgctggtga taaaataaag ctttaataat
      781 actaggtgct agatgacaaa aatatatgat caatggatat tcaagatgca atgatacaaa
      841 aataaaggaa attgaagcaa atgaagcaat agataaaaga tgttgtaatt cttgagaatt
      901 aatataattt aattcatatg cttctttgat cattttaaca ttagttggat aaccacaaaa
      961 aaatgagatg aagtatatag aagacatgat tggtgataat ttaaataatg gtttaaagat
     1021 ccattggatt aaatagctaa agagatttaa taaccctaaa gataaacaga gtgaaataag
     1081 gatcataaat ggtaataatg aaggtaataa attattaata attattttaa tcacattatt
     1141 agagagtgaa acaatttgaa acatgttaaa gaatgaaaat agtgtaataa aggcaactaa
     1201 tagtaaagta aaatagtaat tgaatttttt catatgcttt ccttctagta atataagatt
     1261 agataaggta aaatggagtg gggtgaaatg atgaaggcat taatagtcgg tgttagatat
     1321 aaagaaatga attatgattt agataattca ttaattgaat tagaggaatt atgtaaagca
     1381 tgtaatattg aagtgataaa aagatgtgtg caaaatcttg ataagattaa tccttcatta
     1441 tatattggta ctggaaaggt tcaagaaatt aaaggacagt tagataatct tgacctagtg
     1501 atttttaacg aagaattatc accactacaa gtaaaaaatt taacagatat tttagatatt
     1561 gaagtaactg accgaactgc tctaatttta cgaatttttg aagttcgagc gaaaaccaaa
     1621 gaagcaaaac tgcaagttga aatagctaaa ggtcaatatt tattgccacg tctagcgggc
     1681 atgaaagaac atttatacag ccagcagggt ggttcgggat tcagaggaag cggtgaaaaa
     1741 caaattgaat tagatcgtcg tttggtttca aatcaaatct ttaatgctaa aaagcaatta
     1801 gctaatattg ttaaacagcg tcaaaatcaa cgaaaaagac gaaaagacaa tgagatgaag
     1861 gttattgctt tagtcggtta tactaatagc ggcaaaagta ctttaatgaa tgctttttgt
     1921 ctaaataaaa ataagcaggt attgcaaaaa gatatgctat ttgcaacatt agaaacagcg
     1981 acacggcata ttacgatcaa tcagcacccc tgtttattga cagatacagt tggttttatt
     2041 gagcgtttac cgcatcattt gattcaagct ttccgttcaa ctttagaaga agtagttgaa
     2101 gccgatttgt tgattcatgt tgttgatgct tccaacccta attatgagca gtatattaat
     2161 actactaatg cagttttaaa agaattaggg ataaaggata cgccgatgat ttatgcatat
     2221 aacaaagttg atttaaataa atatggcttt attgtaccgt tagagccata tgcatttatt
     2281 tctgctaagg aacgtattgg actagaagta ttagaaaagt caatcagcga tatccttttt
     2341 aaagattatg caatctatga tttaaatatt ccttatcaag atggtgaagt atttaagtat
     2401 ttacatcagc attgtttagt gttagaattt cagtatttag aaaatagtat ttatatgaaa
     2461 atcgaagctc atccaagatt tatggtacag tatgatcaat atttattaaa acattaaagg
     2521 aggttttgaa atggataatg aaaagaaatt gtttcgctta gatctatcaa ttgctgtaga
     2581 agcaacttca gctcaagaag cctttgatat tttagtgaca gacgaaacat taaaacaaat
     2641 tagagagttg gtaatcaagt cgaaagataa tattaaagaa atgtttgaaa aagaagatag
     2701 tgaacctgca ataatcaatt agttgattat tgcttttatt tttgttagaa ttaagaataa
     2761 aattataaag ggggatttat aatggatttg tatggtagaa attttttaac acttaaagat
     2821 ttttcaaaag aagagattag atattttctt gatttagctc atcagctaaa aaaagataaa
     2881 aaggaaggaa aaacgagtaa tagcttagcg ggtaagaata ttgttttgtt atttgaaaaa
     2941 acttcaacga gaacaagatg tgcttttgaa gtggcagcat tagatgaagg ggctcatgtt
     3001 acttttttaa gtaattcaca aatggggaaa aaagaatcaa ttgaagatac tgccaaagta
     3061 cttggaagaa tgtatgatgg aattgaattt agaggatttg aacaaagtac agttgaagcg
     3121 ttagcaaaat attcaggaat accagtatgg aatggattga cagatgtaga tcatccaact
     3181 cagactttag cagacttttt aacgattgaa gagcatttag ataagccgct taacgaagtg
     3241 aagtttgttt ttacgggtga tattcgtaac aatgtttgtt atggtttgat gtatggagca
     3301 gctaaaatgg ggatgcattt tgttgcatta gggcctagtg aactacaaat ggatccggaa
     3361 gtagtagcct attgtaaagc tgaggccgaa aaatctggtg gaagttttat ggtgacagat
     3421 aatgttgatg aagcggtaag agatgctgat gttatctata ctgatatttg ggtttcaatg
     3481 ggtgaagcag aagaattata tccacaacgg gtgaaattat tatcaccata taaagtaact
     3541 caggcaatga tggctaaaac aggtaatgat aaaactttat ttatgcactg tctaccttca
     3601 ttccacgatt ttgaaactac tcttgcaaaa gaaaaatacg aacaaggtat tgatattcgt
     3661 gaagttgaag atgaagtctt tagaagtgct aattcggttg tttttgatga agcagaaaat
     3721 agaatgcata ctatcaaagc agttatggta gcgactttag gtaaataaaa acggagctaa
     3781 atagttccgt tttctttggt tttagatata ttttcatttg taatcatatc taataaccca
     3841 cgataattgt atgttgcata aacacttggt gtaacacagt gtactggcac ggtattttta
     3901 acaatattca ttgcctgtgg ttctaagtat gaaatttgtg gtgctagata aattgcatca
     3961 cagtccaaat aactactatt taactgatag aagccaagag gaataatttc ataattaagg
     4021 tgttttaagg agattagttc agctagctta tttgcaaaga atgatgatga taaaccgcct
     4081 gagcaacata aaattatttt aagtggggga cattttgtat gtttggtcaa tgaatcgtag
     4141 aagtcgtaaa ataatttttg tccttgagct aaactaacga gtttgtaatg aagatagaat
     4201 aataaatttt cttcatcatc aaagatttgt tcttcaatga taccattagc ccaaataaca
     4261 attgaaccac taatattata ataattaaaa gtgatagttt tcccgtaagg ttcattgctt
     4321 tttaatgtac atagtatatt atgatcatac ttatttttat ttccaaataa ccattgtaca
     4381 aacaattcaa gattaacgtc caaatagaat tcgtcgacca taattatccc ctccttacaa
     4441 atataagtat aatcaaaaat aaaggtaaag acaaattttt tctaaaatag taaagcgctt
     4501 acaaataaat caagtgatat tttaatgcta aagtatgata tgataagatt aggtgataaa
     4561 atgacgggta aaacttatgt catcagtgat ctacatggtc actttgactt attgataaaa
     4621 ttactagaaa cgatatcatt tagtgaaaat gatattttat atataattgg tgatatttgt
     4681 gatcgtggac ctgacagtct aaaaatatta ttttatattc aagatcataa taatattatt
     4741 ttaataaaag gaaatcatga atacatgtgg caagaagctt tatattatgg agtttattat
     4801 gatgattttg attatccttc acgggcgtta aaattatggt tagccaatgg tggtgataca
     4861 acgatgagaa atattcgtga atatcttcaa aaagataaat ttagatatga agattatcgt
     4921 gttatccgga cagctttttt taaaagtctg tatgaatatt tgattaatct tcctaattat
     4981 ttagaaattg aggtaaatca acaaaaatat gtgttagttc atgctggaat cgatgttcgt
     5041 tatccactaa aagaacaaaa attaaattct cttttatgga ttcgagatga tttttatcat
     5101 gcacgaggtg atttgacaaa gatttatatt tttggtcata ctccaacagc gatgttaaac
     5161 aaggatcgga gttttgatat ttgggttgat gatgtccatc aaaataaaat tggaattgat
     5221 ggtggtttgg cttgtggtgt gattggacag ttaaattgtt tatgcctaga tgatggcaaa
     5281 attattattg ttaataagga ggagtaggga atgcgcggag ctttttataa tttagttaat
     5341 tttattaata caacgagcat aaatgatgtt tatgctaatg cagcaagaaa aattcttcaa
     5401 aatatctatg tgattccggg aagtacaata actgatgtag cagagctttg ttttgtttca
     5461 acagcaacga tttcccgatt atgcagaaaa ctaaattatg aatcatttgc tgattttaaa
     5521 atggatatta ccatgaattt aaattatttt aatcgcgata cactcagatt acagtttgat
     5581 catcagttgc cccaaaggca gtatttacat aaaggtaaag aagtgtttaa agctcatttt
     5641 gataatattt tagataattt aaaagaaaca tacgagagtg tatcttatga agacctggaa
     5701 tttattgtcg ataaaattca tgatgcaaat aaggtttgtt ttgctggaaa cttttttact
     5761 caatcagtat cgatgcaatt acagattgaa ctatcatatt tgggtaaaga atgtagtggg
     5821 atgtatcctt tagaacagca aattttgacg gtagaaggat tagatgaaaa tgatgtaatt
     5881 attgtttcaa gtattgcagg aggatttatg actgatcatc cagatgttat gcgagtaatt
     5941 tctaagagtc cggcctataa gattgttatt tctcaattgg atgaatttgt ttatagtgat
     6001 agtatcgata tgatcttaaa ggttggaaca gatcatcatt cactgattgg taaattttca
     6061 ataacctata tttttgaggt tttagaagcc ctgtatcatt taaaatacgg agttaaagaa
     6121 aaacgggaat tataaaaaaa ggactaccat ttggtagtcc taaatcgtat tataagccca
     6181 catagctttg atttgaggag ctaattggct atatgaccat tgcttatgac tattttcttt
     6241 actgttagga tcattaacta aaaacttgtc attaatagtt ttagtaatga caataaagtg
     6301 accagtcgtt gtaaaatcgc caggacgtaa actggcgatg attggatgac cagcatctaa
     6361 ttgcttaaac atttcatttt tgctcaatgg cacagcagaa gcgattaagc caaattcatt
     6421 acagccgctt tccatcaatg cccatcttgt catactgcct tcaagaaaat cgttcttaat
     6481 agcatattta gcaacatagc gtgggttata agttgttttg ccagttaagc caacagcaac
     6541 catcgataga caggtagggc cacaaccggc agttccaata atttcatcac catatgtagt
     6601 atatgcccag cgcttatccc attggatata taaagggatg ccattaggac tatctaagat
     6661 aataggatcg gtgttttcat tgggttgata attttctttc agttcaattg cttgaggatt
     6721 tttatcaaca aattcattaa tgtattgggg gcagtctacc ttatcaagtg cacctgaatt
     6781 atcattgtat agtaggggag ggattatttg gagacataag taaccaaata taaatataat
     6841 gataaaagcg ataaaagcag gaattttttt taatttttta agtttcataa atgcattgaa
     6901 tagtatttaa ttacaattga aaatatagta attaccccca aaaccaatac aataaagaat
     6961 ttttcttttt tatttaattt tttttccata atattgctcc ttaaatgatg aatgtagtga
     7021 gaagaagggg agcgtagtta taaacagaaa ttaatccgat actcccgaaa ctaagtaaga
     7081 ataaactagt cgaaacatta ttttgacgat gctgattaat aaaatgatag attattattc
     7141 caattaaaca gccaagagta tttaggaaaa tgtcatcgat atcaaaaact ccccatccgg
     7201 tgcctaattg aattagttca aaagatagac tagtaaaaaa accaataaag gcaatgttgt
     7261 aattatttag aaaactaaaa tagtgtctca agataatact taaagggata aatagggaaa
     7321 cattgccaat aatatttaca agtagaactt ttgttagccc tgactggtga tattgattaa
     7381 tgaaatcaaa taacactaga ttactattat agcccatatc aataaaacca ttctttaaaa
     7441 tgattattcg tatcagcatc aaaaaataat atattgaaca aagagctatt gtgatgttga
     7501 tcaaatagtt atttttcttt tccattttta tcaccccaat caaaaaacct attctacaat
     7561 gattattata tcaagtgaca atagaatata tatttagata ctctaaagaa aatcttacaa
     7621 atttcttaca gcgaagtata tagaaaatgg tctaaaaaga agctggctat ataatataaa
     7681 aataaaatta taaatagagt tgaatttaaa gtcattttat aaaaaaaatc atatagtatt
     7741 ttgagggggt gaataaatgg ttagagaagg tattgatgtt tcacaatatc aaggtcaaat
     7801 tgattgggag ttagttaaaa atcatattga ttttgcaatc ttacggtgtg gttatggtnn
     7861 nnnnnnnnat attcctggtc aagacgatcc tacttttaaa agaaatgctg atgagtgtac
     7921 acggttagga attccttttg gggtatattt gtattcttat gcaacaactg aaagtgaagc
     7981 attgtctgaa gcacgacatg ttatgcgatt ggttgaagga tataagatgg aatacccagt
     8041 atatttagat ttagaagatc caagaattgg acaattatcg aatgaacaga ttgaaagaaa
     8101 tagtcgtgta tgggccgaag aactagtaaa caatagatat tttccaggat tttatgcatc
     8161 atattattgg tggacagaaa aattaactgg acctttattt aatcgctata caaagtgggt
     8221 agcacggtat gctgaagaat taggggccga aggttttgat atgtggcaat atactgataa
     8281 aggatttgtt gaggggatta atgcaccggt tgatcgaaat tatgcttata gagattttcc
     8341 tgcagaaata atagcaggtg gatttaataa ctttgaaggt gatgaaacaa ttcctccgat
     8401 tactaattat caggtaggag atcatgttac attcgatcat gtgtttgttt cttctgacag
     8461 cacgacacca ttgattccat atataaacca cggaacgatt cgaagagtcg taaagggagc
     8521 gagaaatcca tatttgattg gaaatggact cggctgggtg aataatgata gtataacggg
     8581 aatgatgcag tatttatcta atccgaccta ccgaggagat tcttttgtag atgcattggt
     8641 ccaaatcggg gtagatgctt cattcgcatc tcgaagagag ttagcaagaa aaaatgggat
     8701 atatgattat actggaagtg caaaacaaaa tttagaactt cttcgtttgt taaaggaagg
     8761 tagattaaga caataaagga ttttccttta ttgtttttta ttagaaagat tatataaatt
     8821 aaaagcttta attcctgact tttaggaaat taaagcctat ttatattggt agtttaatgg
     8881 taaatgtaat taaatcatca gttaaatcag cttcaattgt accaccatgg agctcaataa
     8941 tctttttagt aattgcaagg ccaagtcctg aaccgcctgt tttgctatta cgagcttcat
     9001 ctaagcggac aaaaggttga aaaatacgtt ctaaattatg ttcactaata tgtttgacaa
     9061 agtttgctac ctgaataaaa atgcaatcaa cttctttttt ggcagtgatt attaaatctg
     9121 aattttcttt actataatta atagcattac gaataagatt atcaaagact cgttcaaatt
     9181 gattaatatc taattcaaga gacagattgt cttcaatctt ggtaacacag ttgatatttt
     9241 tttcatgata taaaggcata aattcataag agatttgaac taacatcatt gaaagattaa
     9301 cagcttttcg ttctaaagaa atatcactaa gattatattt agcaatagaa aaaaattctt
     9361 cgattaattc ttcaagacga atagctttat cataggcaat tttggtataa ttgctttttt
     9421 cctcgattgt gagattaggc tgattcatta gtaggtctaa atatcctacg atcgaggtta
     9481 gtggtgtctt taaatcatgg gccaagtata gtaataaatc attcctttgt tgttctgctt
     9541 gtttaatcgc ttttttattt tgtaaagatt cacgtttaat ttgattcatt tgatcttcaa
     9601 tttcttttaa ctcatatggt aaactgatca gataattatt atcggcaact aaagtttttc
     9661 ccgcttcaag cattttttca atatactgta aagattcacg gtagcgaagc caaaaaataa
     9721 taatagtatc aaaagcaatc aaagtaattg aaatagtcac taaattatcc ttgatgattt
     9781 gtgctacctg gtagacaaga tcatattctt gccaaatata ctggtgatat acttcataag
     9841 aataaagtaa taaaataaca aaagttacga ccgtgacact aattgtgatt ccaatttttt
     9901 taagtagatt agcagtaaga ctttgcttaa aatgagtatt attcaatagt ataaccaacg
     9961 ccccaaacag tttttacaaa tttgggtttt cttgcattct cattcatttt ttcccgcagt
    10021 ctagcaacat gagccattac ggtattattg ttatctaaat atttttcttg ccatactgct
    10081 tcaaaaagtt cttctgatgt aacgacactg ccacgttttg aacataaata ccaaagaatt
    10141 gaaaattcaa ttggagtcaa atttaatggc tggtcatata atgtacactt atgattatca
    10201 ttattgatca ttaaaccatt aaaatcgtat atttgttgat taaccgctgg ttcgttagtg
    10261 ttataacggg tataacgacg taattgagtt ttaacacgag ctgccaattc tagaggatta
    10321 aagggtttag ttaaataatc atcagcgcct aaggtcaaac cgttaatttt atccatgtca
    10381 ttatttttag cagtaagcat aataactggg aaaaaataat cttcacgaat tttttttaat
    10441 agactaaacc cgtctaaatc gggcatcatt acatcaagaa ttgctagatc ataatgattt
    10501 tttttcaagc tgtctaaagc ttcttgacca tgataaaatt taaaaacttg ataactttca
    10561 ttttgtaaat a
//
LOCUS       DAOMTA010000011        10263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_41_length_10263_cov_2.483444, whole genome shotgun sequence.
ACCESSION   DAOMTA010000011 DAOMTA010000000
VERSION     DAOMTA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41_length_10263_cov_2.483444"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLK68_00745"
     CDS             complement(<1..263)
                     /locus_tag="PLK68_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88967.1"
                     /translation="MITFIKKIISGQKKLYLGILAVLSLLSSFEFISVVMYSTSNIEV
                     DFANGYILQLVTGIVIMIAFALTLFVNNYIINNKNEEFSLLLL"
     gene            complement(418..1596)
                     /locus_tag="PLK68_00750"
     CDS             complement(418..1596)
                     /locus_tag="PLK68_00750"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HRM88968.1"
                     /translation="MYIDIEHFKYINDVFGYQFGDKILKKYAAVLANSIDKNETFARN
                     NADSFIILRHYESKEMLKQKQIEIDQILIDYVKKAKNGYQIRLCCGICCYEDVIEKLT
                     IDEYIDRAIFAQDTIKRTSEEHYAFYTEAIRNKLHMEKMIENRMQTALDNQEFLVYLQ
                     PKVNIKTGKIACAEALVRWKSEDRLIYPDLFIPIFEKNMHIKEIDKYVYREVCSWIRK
                     RIDEELPCYPVSVNVSKVQFYNSKFVNEYYQIKQEFGIPDRMIEIEFTESVAFERTDL
                     LIKIINDLRNYGFICSMDDFGKAYSSLNLLKDLPIDVLKLDSAFFEDSDNPDKVKLII
                     KDIIKMVNNLGIKTVAEGVEQVSQVEFLREVGCDLVQGYVYYKPMPINEYEKLIDKNS
                     "
     gene            complement(1581..2252)
                     /locus_tag="PLK68_00755"
     CDS             complement(1581..2252)
                     /locus_tag="PLK68_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88969.1"
                     /translation="MRLKRLGLPAFLLTLILLCSISVHTLYKIRDYGTLINYVGIVRG
                     ASQRLVKLELTDQPNDELKDYIDEILTELDMGVGKYSLINLDDEDYRTDLNKLKNIWE
                     AIQMDIVLVREGNGHEKLLSDSEDLFEIANDTVFAVDYYTTTQSNYLLMMLMILSGIS
                     LVTWSIIIIFYLRSMITLQRKNNTLSIVAYRDNLTGVNNRLKFVLEAILLRKQKVNLL
                     FCILI"
     gene            2464..3915
                     /locus_tag="PLK68_00760"
     CDS             2464..3915
                     /locus_tag="PLK68_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRM88970.1"
                     /translation="MKTRNLIATSSLALTMMLGIVATPLSAQQTYTPGVTVEKNTNPE
                     WEADYTATFIYKDKDARDATNVSVSGGFQFYKPEEVQSFANTGDGANIPCYNAYQYQD
                     GMFAGGYNLNGDAANYSMTEIEDEIFEVTIPLPANEYFYAFNIEYSDGSSETNVKDPA
                     NPALANDGSDAGWSLFYVGNGETKGQEYINPRTDGKTGTYSFVNYLAEDGTTQPLGIY
                     LPNGYDANKTYKTIYVSHGGGGNEVEWMTIGAVPNIMDNLIADSLTKEAIVVTMDNTY
                     FNWDYDRIINNLFNNILPYIETHYNVSKEVNDRAFCGLSMGSMTTTTLYQTHPDQFGY
                     FGCFSGANVPVDVSKVAHLDQPNLYITAGCIDMALMNDSYNTASDRTTTGFVDKLNTL
                     NLTNYKLEILNGAHDWYVWRESFTNFVKDHLWTKDVKIETPSETPVIKPETPASSTNT
                     TTAPKTGDDLNLAVAGLFIIISVTTGVVIKKHY"
     gene            complement(3969..4778)
                     /locus_tag="PLK68_00765"
     CDS             complement(3969..4778)
                     /locus_tag="PLK68_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM88971.1"
                     /translation="MKRHEQLGFNFFRYCNENLSNTNEYNIARIIIEHIGDIKTVSLE
                     QIAQEANISIASVSRFVQKIGYSSFQDFKDGLDYFIRNLNMVRTVSNMQQFMRTSLDN
                     LADSLYVEAISNLRQTKLNLDMEKLVAITKLLLNSRSVTFIGDTHEMIDFYTVQLDLV
                     ANEVPAYLFDLQEFQDIHSDFFKDGDTLVLLNVSNDFYSEIQKRVVEKASQKNLKLVV
                     FAQDDLAEQKIFDYIYQYGIPKSINDGYYSLFYLSQIISELIYKVRVIKKS"
     gene            complement(4880..5680)
                     /locus_tag="PLK68_00770"
     CDS             complement(4880..5680)
                     /locus_tag="PLK68_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM88972.1"
                     /translation="MFNKALFKLEWKSNYKILVIFCLILTMYTTIMLAMYDPKLGTAL
                     ETFAKSMPEIMAMVGMSGTPTTLIDFLSTYLYGLIMIVFPLIFGILLALRLVVRKVDN
                     GVMSYLLCSGVERRSVWFTQMLVIITNLFVLIAFCTGLGLGCSALMFPGDLDIGAYLV
                     LNLGVFILQLTLTGICYMCSCIFNEYRLASLFGAGIPIVFIMIQMLSNMQGSMEGLKF
                     ATLLTLFDPQKLINGNNEGYLMLGGLLVVMIVCYGVGGIIFDRKSMSL"
     gene            complement(5670..6563)
                     /locus_tag="PLK68_00775"
     CDS             complement(5670..6563)
                     /locus_tag="PLK68_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM88973.1"
                     /translation="MSVVEINGLTKDYGNDKGIFDVSFKIEKGEVFGFLGPNGAGKTT
                     TIRHLLGFIIPKEGTCLIEGMDCSKKIAEIQKKIGYIPGEINLMEEVTGIQFIKFMAE
                     YRGMKDLGRAEEIIERFDLDPHVKIKKMSKGMKQKIGLVVAFMHDPAVFILDEPTSGL
                     DPLMQSEFINLIIEEKKRGKTILMSSHMFEEVEKTCDRVGIIRQGRMVSIEDIDTLKK
                     SKQKIYIITFKNHQDAYKFQKESFTIRNADDNVVEIVIKHEINELISCLNNYELMNFD
                     IAHQSLEEIFMQFYGGNQHVQ"
     gene            complement(6775..7161)
                     /locus_tag="PLK68_00780"
     CDS             complement(6775..7161)
                     /locus_tag="PLK68_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM88974.1"
                     /translation="MNQKFFELNEEKRLAIINAGLEVFSKNDYKHALTDDIAAKAGIS
                     KGLLFYYFHNKLELYQYLAQYSARLVMKLFEKMNIIDGKDFFDAIKLAVLGKMEMMSK
                     YPYIYGFSLRYYQNRKEIVMNNYMQR"
     gene            complement(7303..>7801)
                     /locus_tag="PLK68_00785"
     CDS             complement(7303..>7801)
                     /locus_tag="PLK68_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM88975.1"
                     /translation="YRQQAATLLIEAFPYAYKDCAELEITKCLSTNRIMLGAVENDIL
                     MGLVGAIPQYGITAWELHPLAVSKKWQGQGIGTKLCTALENELKNCGCCTIYLGSDDE
                     FDKTTLANTNLFDDLYSKIKNIRNLERHPYEFYQKIGYQIVGVIPDANGLGKPDIWLA
                     KSLVR"
     assembly_gap    7802..7811
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8722..9717
                     /locus_tag="PLK68_00790"
     CDS             8722..9717
                     /locus_tag="PLK68_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRM88976.1"
                     /translation="MRNLDKSISINGLTLKNRLVMPPMATSSANDGEVSQRILDYYDK
                     KTKGGYIGLVITEHSYIDIQGMANPKQMSVAKDSDIKGLKQLVNIIHNNGSKAFAQIN
                     HAGSMARGTGLPTVSASNTIPITMKESNIEIPEELSKEQIQYIVKRFADAARRVKLAG
                     FDGVEIHSAHAYLLNQFYSPITNHRTDEYTGTTLEGRLRIHKEVIEAVRSEVGEDFPI
                     ALRLGGCDYMAGGSTIKDSIKASQMLESYGVDILDITGGINRYMIPWNKEPGYFSDMT
                     EHIMEKVSIPVILTGGITNAMDAEKLLQLNKADLIGVGRAILKDDSWAKNAMEQK"
BASE COUNT         3660 a         1802 c         1293 g         3498 t
ORIGIN      
        1 caaagcagta ataatgaaaa ctcctcgttt ttattattaa taatgtaatt attcacaaac
       61 aaggtcaatg caaatgcaat catgattaca attcccgtga ctaactgcag aatatagcca
      121 tttgcaaaat ccacttcaat attactagtt gaatacatca caaccgaaat gaattcgaat
      181 gatgataaca gtgataaaac tgctaatatc cctaaataca gttttttctg cccggaaata
      241 atttttttaa taaatgtaat catttccaaa tcctcccttc actaaattct ttatctactt
      301 taagtatacg cttacatttt ttaaattaca aatattatta taattatttt taagtgaata
      361 actattattt attttaataa aacagcccct acaaatgata catatcattc atatgggcta
      421 actattttta tcaatcaatt tttcatattc atttataggc attggtttat agtacacata
      481 tccctgaacc aaatcacatc ctacttcacg caaaaattct acctgcgaaa cttgttctac
      541 accttcagca accgttttaa ttcctaaatt attgaccatc ttaataatat ctttaataat
      601 taatttaact ttatccggat tatctgaatc ttcaaaaaaa gcactatcca actttaaaac
      661 atctattggt agatctttca ataaatttaa tgaagaatac gcttttccaa aatcatccat
      721 agaacaaata aaaccataat ttcttaaatc attaataatt ttaattagta aatctgttct
      781 ttcaaaagca acactttcag tgaattctat ctcaatcatt cgatcgggaa taccaaattc
      841 ttgttttatt tggtaatact cattcacaaa tttagaattg taaaactgca ctttagatac
      901 attaaccgaa acaggataac atggtaactc ttcatcaatt cgttttctaa tccagctaca
      961 aacttcacga tatacatatt tatctatctc cttaatatgc atattttttt caaaaatagg
     1021 gatgaacaga tcgggataaa tcaatctatc ttcactcttc cagcgaacta atgcttctgc
     1081 acatgcgatt ttaccagttt taatattgac tttaggttgc aaatatacaa gaaattcttg
     1141 attgtctaaa gctgtttgca tcctattttc aatcattttc tccatatgca atttatttct
     1201 aattgcttca gtataaaaag catagtgttc ttcagaagtt ctttttatag tatcttgagc
     1261 aaaaatagca cgatcaatgt actcatcgat agtaagtttt tcaataacat cttcataaca
     1321 gcaaatacca caacaaagcc tgatttgata accattcttt gctttcttta cataatcaat
     1381 caaaatttga tcaatttcta tttgtttttg ttttaacatt tctttactct cataatgacg
     1441 caatataata aaactatcag cattatttcg agcaaaagtt tcatttttat caatactatt
     1501 tgctaataca gctgcatact tttttaatat tttatcacca aattgatatc caaagacatc
     1561 attaatatac ttaaaatgct ctatatcaat atacaaaaaa gcaaattgac cttttgtttt
     1621 cttaataata ttgcctctaa aacaaattta agtcgattat ttaccccggt caagttatca
     1681 cggtaagcta cgatactcaa agtattattt ttgcgttgca atgttatcat gcttcgtaaa
     1741 taaaaaataa ttattataga ccatgtcact aatgaaattc ctgataaaat catcaacatc
     1801 attaatagat aattactttg tgtcgtagta taatagtcaa ctgcaaagac agtatcgttt
     1861 gctatctcaa ataaatcttc actatcacta agcagtttct catgaccatt tccttcgcgt
     1921 actagaacta tatccatttg gattgcttcc caaatatttt ttaatttatt taaatctgtc
     1981 cggtaatctt catcatcaag attaattaga ctatatttac caactcccat atctaactct
     2041 gtaagaatct catcaatata atcttttaat tcgtcatttg gctgatcagt taattctaat
     2101 ttgaccaaac gctggctcgc accacgaaca atcccaacat aattaatcaa tgtcccataa
     2161 tcacgaattt tatataaagt gtgaactgat attgagcata atagtattaa tgttaataaa
     2221 aaagcgggta accccaatct ctttagtctc atctctatca caccctatca ccttaatgat
     2281 atgctaatca ttaggataaa acaagcaaat gccctcattt ttacattttt tgtaaaacgc
     2341 ttacattttt atcaagtgat ttacacaaga aaagatttaa atatcaaatt tttactttta
     2401 taatacaatt aacatatatg tttaaaacaa ctagttgcaa aatatgattg aatggagaat
     2461 atcatgaaaa caagaaactt aatcgcaaca tcatcattag cacttacaat gatgctagga
     2521 atagttgcca cccctttatc agcacaacaa acatacacac ccggtgtgac ggtagagaaa
     2581 aatactaatc cagaatggga agcagactat acagcaacct tcatttacaa agataaagat
     2641 gcgcgtgatg caacaaatgt tagcgtaagc ggtggtttcc aattttataa acccgaagaa
     2701 gtacagtcat ttgcaaatac tggtgacggg gctaatatcc catgttataa tgcttatcaa
     2761 tatcaagatg gaatgttcgc tggagggtac aatcttaatg gtgatgccgc aaattattca
     2821 atgactgaaa ttgaagatga aatctttgag gtaacgatcc ctttacctgc aaatgaatac
     2881 ttttatgctt tcaatattga atattcagat ggcagtagtg aaactaatgt caaagatcct
     2941 gctaatcccg cacttgctaa tgatggttca gatgctggat ggagcctatt ttatgtaggt
     3001 aatggtgaga ctaaaggaca agaatatatc aaccctcgta ccgatggtaa aacaggtact
     3061 tactcatttg tcaattattt agctgaagat ggaactactc aacctttagg aatctatcta
     3121 ccgaatggtt atgatgctaa taaaacatat aaaacaatct atgtttctca cggtggcggg
     3181 ggtaatgagg tagaatggat gactattggt gcagttccaa atatcatgga taatttaatt
     3241 gctgatagtt taactaaaga agcaattgtt gttacaatgg ataatacata cttcaattgg
     3301 gactatgatc gtattattaa taacctattt aataatattt tgccttatat cgaaacacat
     3361 tacaatgtat caaaagaagt aaacgatcgg gcattttgtg gattatcaat gggatctatg
     3421 acaactacga ctttatacca gacccatcct gatcaatttg gatattttgg ctgcttttca
     3481 ggagctaatg ttccagttga tgtaagtaaa gttgcacatc ttgatcaacc aaacttatat
     3541 attactgctg gctgcatcga tatggcctta atgaatgata gttataatac tgcgagcgat
     3601 cgtactacaa ctggattcgt agataagcta aatacattaa atctaaccaa ttacaaatta
     3661 gaaattttaa atggtgctca tgactggtac gtttggcgtg aatcgttcac taattttgta
     3721 aaagatcact tgtggactaa agatgttaag atagaaacgc ctagtgaaac tcctgttatt
     3781 aaaccagaaa cacctgcttc atcaacaaat actactactg cacctaaaac aggtgacgat
     3841 ttaaatcttg ctgttgctgg attatttatc atcatctcag tgacaactgg tgtagttata
     3901 aaaaaacatt actaaataca ataaaaatcg taagttcatg atatctctta acaaagaaga
     3961 tatcaaactt acgatttttt tattactcgt actttataaa ttaattcaga aataatttga
     4021 cttaaataaa ataacgaata atagccatca ttaattgatt taggaatgcc atattgataa
     4081 atataatcaa atattttctg ctcagctaaa tcatcttgag caaaaacaac aagttttaaa
     4141 ttcttctgac tagctttctc tactactcgt ttttgaattt cactataaaa atcattggac
     4201 acatttaaca aaaccaaagt atctccatct ttaaaaaaat cactatgaat atcttgaaat
     4261 tcctgtaaat caaataaata tgcaggaact tcattcgcca ccaaatctaa ctgcacagta
     4321 taaaagtcaa tcatttcatg agtatcacca ataaatgtaa cactacggct attaagcaat
     4381 aatttggtta tagcaacaag tttttccatg tccaaattaa gttttgtctg acgaagattg
     4441 ctgatagctt caacatataa agaatcagct aaattatcca atgatgtacg cataaactgc
     4501 tgcatattag ataccgtcct taccatattt aaattacgaa taaaatagtc taaaccatct
     4561 ttaaaatcct ggaaagaact gtatcctatc ttttgaacaa agcgactaac cgaagcaata
     4621 gaaatatttg cttcttgagc aatctgctct aatgaaacgg ttttaatatc accaatatgt
     4681 tcaatgataa ttctagcaat attgtattca ttagtattag aaagattttc attacaatat
     4741 ctaaaaaaat taaatcccaa ctgctcgtgt ctcttcataa attccctcca ttttttatat
     4801 tatatcaatt aaatatctta attaaaagga attctcttta aatgtctaga cacataaaaa
     4861 taaccgggac cggttatttt tataaagaca tacttttacg gtcaaaaatt attccaccta
     4921 ccccataaca aacaatcatt actacaagta atcctcctaa cattaaataa ccttcattat
     4981 taccgttaat caatttttga ggatcaaata aagttaataa tgttgcaaat ttaagtcctt
     5041 ccatacttcc ttgcatattc gaaagcattt gaatcataat aaaaactatc gggattcccg
     5101 ctccaaataa cgatgctaag cggtactcat taaaaataca agaacacata taacaaattc
     5161 ctgttaatgt taactgtaaa atgaaaactc ctaaatttaa aaccaaataa gctccaatat
     5221 ccaaatcacc aggaaacatc aaagcactac atccaagccc taaacctgta caaaaagcaa
     5281 ttaaaacaaa taagtttgtt atgataacca gcatttgtgt aaaccacaca gatctacgtt
     5341 caacgccact acataataaa taactcatca caccattatc aacttttcga acaactaaac
     5401 gtaatgctag taaaattcca aaaatcaaag gaaaaacaat catgattaaa ccatataaat
     5461 aagtagagag aaaatcaatc agtgttgtcg gggtaccact cattcctacc attgccatta
     5521 tttcaggcat acttttagca aatgtttcta gcgctgttcc tagcttagga tcatacattg
     5581 ctaacatgat cgttgtatac attgtcaaaa tcaaacaaaa aataactagt atcttataat
     5641 tagatttcca ttctaattta aatagcgctt tattgaacat gttgatttcc tccataaaat
     5701 tgcataaaaa tttcttctaa actttggtgt gcaatatcaa aattcattaa ttcgtagtta
     5761 tttaaacaac tgattaattc attaatttca tgctttatta caatctcaac aacattatca
     5821 tcagcatttc taattgtaaa agattctttt tgaaatttgt aagcatcttg atgattttta
     5881 aatgttatta tatatatctt ttgtttcgat ttctttaatg tgtcaatatc ctcaatactc
     5941 accattcgtc cttgacgaat aattcctacc cgatcacatg ttttctctac ttcttcaaac
     6001 atatgtgagg acatcaaaat agtcttacca cgcttctttt cttcaataat taaattaata
     6061 aactcacttt gcattaaagg atcaagtcca ctagttggtt catctaaaat aaaaacagct
     6121 ggatcatgca taaaagctac taccaaacct atcttttgtt tcatcccttt tgacatcttt
     6181 ttgattttta catgaggatc aagatcaaag cgttcaatta tttcttcagc acgccccaaa
     6241 tccttcattc cccgatactc agccataaat ttaataaact gaattccagt aacctcctcc
     6301 attaaattaa tttctcctgg aatataacca atcttctttt gaatctctgc aattttctta
     6361 ctacaatcca tcccttcaat taaacaggtt ccttctttag gaataataaa ccctaacaaa
     6421 tgtctaattg ttgtagtttt tccagcacca ttaggtccta aaaagccaaa aacttcacct
     6481 ttttcgattt taaaagagac atcaaatatt cctttatcat taccataatc ttttgttaaa
     6541 ccattaatct caacaacact catataaatt cctcccgata gaaattctct tttataatat
     6601 ccaaatatct aaaatactca tcttgaatct ctttcatatt aacattttct aaatcctgat
     6661 atctatccat atatccttgc gacatccaat aaattatgtt ccaaacttct tcaggatcaa
     6721 ctcctgcttt aaacttacta atatcagcac gtttaattaa attatatgat gctattacct
     6781 ctgcatataa ttgttcataa ctatttcctt acgattttgg taatagcgca gtgaaaagcc
     6841 ataaatatat ggatatttag acatcatttc cattttgcct aacactgcta atttaattgc
     6901 atcaaaaaaa tctttaccat ctataatatt cattttttca aataacttca tcactagtct
     6961 agcgctatat tgtgctaagt actgatacaa ctctagttta ttgtgaaaat aataaaacaa
     7021 tagtcccttt gatattcccg ctttggctgc aatatcatcg gttaatgcat gtttataatc
     7081 atttttacta aatacttcta accctgcatt aataatggcc agtctttttt cttcgtttaa
     7141 ttcaaagaat ttttggttca ttttatcacc cctttgactg acttgattaa ttaaattata
     7201 gcaaattgac tcgtttcaac aacccctcgt atcaatcttt atgcaatctt aatagtccaa
     7261 aaaataaaga ccaaaatgaa atattttgat cttttcatca tcctatctta ctaaactctt
     7321 agctaaccaa atatctggtt ttcctaaacc attggcatca ggaatcaccc caactatttg
     7381 atatcctatc ttttgataaa actcataggg atgtcgttca agattcctaa tattttttat
     7441 ctttgaatat agatcatcaa ataaattagt attagccaat gttgttttat caaattcatc
     7501 atcactgcct aaataaatgg tacagcaacc acaattctta agctcatttt ctaatgctgt
     7561 acaaagcttt gtaccaatac cttgaccttg ccacttttta gaaaccgcca aaggatgcaa
     7621 ttcccaagca gtgattccat actggggaat agccccaacc aaccccatca atatatcatt
     7681 ttcaactgcc cccagcatta ttctatttgt tgataaacat tttgttattt ctagctcggc
     7741 acaatcttta tatgcatatg gaaatgcttc aatcaataat gtagctgcct gctgacgata
     7801 annnnnnnnn nggttaagtt agatgttaac ttaattattt ccatattttc acccctttta
     7861 cttatataat accatctatt ttactttaag ctgcttaatt cttattttta tattattaat
     7921 aatataaaaa taggctgtaa ctaaatagaa taaatttatt ctagaggggt tatacctctt
     7981 ttttatttca actgtttttg gatattcata aatgtaatgt aaatatcttt atttttaact
     8041 attataaata attatttctc ttttcgatac tttctatgtt ggttttcaag aaatgtgata
     8101 ctagaataaa aactgtacca gttaaatgaa aaaaagcaat cagcccttca tgcctacaaa
     8161 gctcatatca ctttcctttc ttaatagaac tgctagctat catatagatt tacctcaaaa
     8221 gtattctaac aaagagtcca gtttttagct atttaaaata cttatttatt aaatcgttta
     8281 tatacatgtc accccaatat catatttcct gcaaaacagg cattaaagac tcccccgctt
     8341 tgaaagatag tattctactt ttggagaaat ttaaggatat tcttttctta tagtgagata
     8401 atcagcctcg agttctttta attgtgtact taacattttg tgtgatatag tccctaatgg
     8461 acgttttaat tcactatatc gcattaattc agttttccaa agctaaaata aaatattcat
     8521 cttctatttg ccacctataa ttgacattgt atattcaaga tggttttgaa ttaactgctc
     8581 catttgttta catctatact tattttttct tccattttaa actctccttt tagatagtat
     8641 cttacctaaa agtgcatact gttatttttg ttaaggtagt gctatattac acttaataaa
     8701 aagaaaaaag gagtaatgat gatgagaaat ttagataaat caatttcaat caacggattg
     8761 acgttaaaaa acagattagt aatgccccca atggcaacat caagtgctaa tgatggtgaa
     8821 gtaagtcaaa gaattcttga ttattatgat aagaaaacaa aaggtggata tattggatta
     8881 gtcattactg aacacagtta cattgatatt caaggaatgg ccaatcctaa acaaatgtca
     8941 gtagcaaagg atagcgatat aaaaggatta aagcaattgg taaatataat tcataacaat
     9001 ggaagcaagg cgtttgctca aataaatcat gccggttcaa tggcaagagg aacaggatta
     9061 cctactgtta gtgcaagtaa tacaatacca ataacaatga aagaatccaa tatcgaaata
     9121 ccagaagaat tgagtaaaga acagattcaa tatatagtaa aaagatttgc tgatgcagcc
     9181 agacgcgtaa agttagcagg ttttgatggg gtagaaattc attctgcaca tgcatatctt
     9241 ttaaatcaat tctattcgcc aataaccaat catagaacag atgaatatac aggtacaaca
     9301 cttgaaggtc gattgagaat tcataaggaa gttattgaag cagttcgaag tgaagttgga
     9361 gaagacttcc caattgcttt acgcttaggt gggtgtgatt atatggctgg cggctcaaca
     9421 attaaagatt ccattaaagc ctctcaaatg cttgaaagtt atggtgtaga tatccttgat
     9481 ataactggtg gaataaatcg ctatatgatt ccatggaata aagagccagg atatttttca
     9541 gatatgacag agcatattat ggaaaaagtt tctattccag ttattttgac aggtggaata
     9601 actaatgcca tggatgctga aaaactactg caattaaaca aagcagattt aattggtgtt
     9661 ggtagagcta ttctaaaaga tgatagttgg gcaaaaaatg caatggaaca aaaataatgc
     9721 aaatactggt atctataata caagtatttg ttgaattacg atacaataaa tccggagatg
     9781 aatactatct ccggaaatgc ggcaatgacc tctaaaaaat ttaaaaagaa agtaagaaaa
     9841 gtttccaaga agttaggaaa aattgcctaa aacttatatt taaaaccatt tttaagatgg
     9901 taattcttct ttaccatatc atatttatta ttaatttaaa atgtatttta aaattataaa
     9961 atttattaca attaaaaaca gataaatttg tatgtaccta agaaaaacat tattcattaa
    10021 tatattagaa aaattataaa ggagaaaata catacctttt atatggggct ttgaatataa
    10081 cggggaattg attacttgtt tgaaatcaca tgccataaga tttataagat aataaaaata
    10141 atgtattttt aagtatatat tttgatattt aatcttattt aaaatataga ttacagtata
    10201 tatgaatagc gatacagctg ataaacaatt atttcttgga ttttgggatt aatataataa
    10261 cta
//
LOCUS       DAOMTA010000012        10142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_44_length_10142_cov_2.587786, whole genome shotgun sequence.
ACCESSION   DAOMTA010000012 DAOMTA010000000
VERSION     DAOMTA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_10142_cov_2.587786"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLK68_00795"
     CDS             complement(<1..382)
                     /locus_tag="PLK68_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM88977.1"
                     /translation="MEKFYQTMLSNIRKHPLLQKIIMGFTRYIPIITFIVYSILLVYL
                     LYTQNTLLAKTLYKPLASFLIVTLLRKVINRKRPYEAMAVDPLIEHKQGESFPSRHTV
                     SAFAIALACLQVNSLLGTIMLILAF"
     gene            complement(392..616)
                     /locus_tag="PLK68_00800"
     CDS             complement(392..616)
                     /locus_tag="PLK68_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRM88978.1"
                     /translation="MFNQIDIHGCTTIEAKIRLDNYLNSLSPNTKEITVVHGYSSKIL
                     QQFIRKQYKHKRAGRRILTMNAGETIIQLK"
     gene            734..1834
                     /gene="ychF"
                     /locus_tag="PLK68_00805"
     CDS             734..1834
                     /gene="ychF"
                     /locus_tag="PLK68_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRM88979.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITNAQVEAANYPFATIDPNVGVV
                     EVPDYRLDKLTELVEPKKTVPTTFGFTDIAGLVKGASRGEGLGNKFLGNIRETDAICE
                     VVRCFRDKDVTHVDGDVDPIRDIETINLELIFADLDTVEKRIGRIGKKAQSGDKEAKL
                     EVAILEKLKSTLEANKPARVIEFSKEEMDVVKQYTLLTMKPIIYVANLGEEDLEDPTT
                     NPHYNKVVEFAAGEGADVVPICAKIESELVGMDKEEKDLFLQDLGIEESGLDKLIKEA
                     YKLLGLRTYFTAGVQEVRAWTFKEGMTAPEMAGIIHSDFQRGFIKAETYSFDDLVEYG
                     SEHALKEAGKIRQEGKQYVGQDGDIMLFKFNV"
     gene            1834..2637
                     /gene="srtB"
                     /locus_tag="PLK68_00810"
     CDS             1834..2637
                     /gene="srtB"
                     /locus_tag="PLK68_00810"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HRM88980.1"
                     /translation="MAKTKKSFIERIKPTGAKDLIRKIILLVCICVFCYSAYNLASIF
                     LEYKSMDDSNKEVEETYVTENTEQKNSYKTIDFEALLARNSDVKGWIDIPDTKVSYPI
                     VQGETNDTYIHSDIDKKEFRAGSIFIASENKNPFTDLNTVIYGHNMKNGSMFNNIKSY
                     TEQDFADKHPYVYIYLPDGTVSRYKVVAAHIIPEESLLYNTGITDIQAFYQEMLKTSD
                     IKVDFEQAAGNPVITLSTCTSAGSESGKRNVVHAVLDRAGIDPKTETMD"
     gene            2884..4380
                     /locus_tag="PLK68_00815"
     CDS             2884..4380
                     /locus_tag="PLK68_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM88981.1"
                     /translation="MKEKILKFITSKFFVLGIQLLATIAVVYFTFKLDLVPTKYLIAG
                     IVVLALLLAGFFGIIYSSEQKIKKGLSSKRGIVTKIISLLTSIILIAGCTYISRGNNF
                     IENVSNATGQEYVVSVISLKNGKITKLKDLDSSKKIGVSYEKDTVTIAEALKDLDTEI
                     GDHEYTKYDNYASLADALYEGKVDAIVVGEQYRTMLQTNHEEFNDVTKVLKSYAYDAK
                     MEVTTKQTDVTENAFSIYVTGIDVYGSLKTVSRSDVNLIVTVNPKTKQILMTSIPRDC
                     QINLHKNGKMDKLTHTGIYGTSETINTIQDLLEMKINYFARTNFSGMTNIVDALGGIT
                     VNSSEAFETLHGNYQITEGLNEMDGDKALCFVRERKRLKRGDFARGENQQKVLKAMLD
                     KAMSPKIITNFNNILSAVEGCFETDMSDKEIKSLINMQLNDMADWKIINVQIEGEYQL
                     MDDTFSMKGTNSDVMIPFESHIERVQELINKVEEGKEIKDSELKGLTH"
     gene            4468..5835
                     /locus_tag="PLK68_00820"
     CDS             4468..5835
                     /locus_tag="PLK68_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRM88982.1"
                     /translation="MVFNRKYYKLCFIIYMFINTLALGYLGIETNYLFVPLLIWALVI
                     IVHDIYKKKFKLKKNYSLLMIVQGLILLLATARNDYSDLNSYVIAVMQLVIYLLIFNN
                     PVSMSKDDIEDEVRMIIPLVNVLVGGASLISIGMYLVHFSSLANGWTLGMVGNRLFGI
                     YFNSNPAAFLACITIVLALVAVRQKFKGKYWYLANIGIQLVYILLTRCRAALIILAII
                     IVMVGYYFLIRRKPYSNFKRLGLVISLIVVIAGASLVGQRVVEIVPQMQGIASKETSR
                     FQMDKVVKAGHLLIAGNWQDFNQGLTIIDEVSNGRVSLTKAALEIWHTEPVIGIGANN
                     FKKIGSQETDALEYWAVQVVHSHNVFLEALVTTGVIGFILFVVFFFKTLLMIFNVLKK
                     SHGKEIYFIVQMFAMIVLSEFIGSLSDYGVFYIYSLSATLAWCFLGYLYTYQNITEIS
                     NIDKI"
     gene            5929..6090
                     /locus_tag="PLK68_00825"
     CDS             5929..6090
                     /locus_tag="PLK68_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM88983.1"
                     /translation="MNNEEQINKVIDYIEEHLQDENLTLTTIAQEIGYLKYHLYRMFT
                     MIAGITIYQ"
     gene            6106..6300
                     /locus_tag="PLK68_00830"
                     /pseudo
     CDS             6106..6300
                     /locus_tag="PLK68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6471..6902
                     /locus_tag="PLK68_00835"
     CDS             6471..6902
                     /locus_tag="PLK68_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRM88984.1"
                     /translation="MKEKRIGIEIRILANLICRCLNELGFNEEHNGLTGPQGLVLGYL
                     YDHQDKDIFQKDIEATFNIRRSTATGLLQCLEGNGFVKRVSVDYDARLKKIVLTTKAH
                     EFKELLESHIQKMEEILVKDLEPQEVDDLIRIIGKIKKNLE"
     gene            6912..>10142
                     /locus_tag="PLK68_00840"
     CDS             6912..>10142
                     /locus_tag="PLK68_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRM88985.1"
                     /translation="MEKKYYMGIDVGSTTIKIYITDQDDNCIYSKYERHYSDIQATIK
                     TLINDVKEQFGNLEVTAVMTGSGGLSLSSCLDVKFVQEVIACTKTIETYIPECDVAIE
                     LGGEDAKITYFQGSLEQRMNGTCAGGTGAFIDQMASLLQTDAGGLNELAKNYQMIYPI
                     ASRCGVFAKTDVQPLINEGANKEDIAISIFQAVVNQTISGLACGKPIRGKVAFLGGPL
                     YFLDQLRQRFIETLNLQDDEIIFPDNSQLFVAMGATLLAKEEEKPTTIEALIEKLDNI
                     DETMLASEDTLDPLFADENDRNGFRHRHYINKVRKNDIKGYVGNVYLGLDVGSTTTKA
                     VLIDDNDSLLYSFYDSNEGNPLDVVVKIVKDIYDFIPPNVKLVRSGVTGYGEALIKAA
                     LKVDMSEVETMAHYKAATYFKEDVSFILDIGGQDMKAIKIKDGIIQDILLNEACSSGC
                     GSFIETFAKSLGYEVSDFAKLALESKAPIDLGSRCTVFMNSKVKQAQKEGAKVEDISA
                     GLSYSVIKNALYKVIKLRSKEELGDSILVQGGTFYNEAVLRAFEKEAGVNAIRPDIAG
                     LMGAFGIARLARESYQGEPTTLLTKDELENFTCQSEIRYCEKCTNHCMLTVSTFNDNS
                     EYISGNRCERGANIPVSSKKLPNLFDYKYRRVFNYRSLTEDQAVRGTVGIPRVLNMYE
                     NYPFWHTFFTKLGFRVILSPRSSKAIYEKGIESIPSESVCYPAKLAHGHIEALIEKGI
                     KYIFYPSLAYERKEFDNANNHYNCPVVTSYPEVIRNNVDNLENRSIIYRNPFMDLSNR
                     KTLFNNLKDELRAFNVNDKELLSAINDAYDELAKCRQDIQDQGEMVLQYLKDNKMTGI
                     VLSGRPYHVDPEINHGLADLITAEGMAVLSEDSVCHLDKDLDQLRVVDQWTYHSRLYR
                     AASFVATQPNLELIQLTSFGCGLDAVTSDQVADILKARHKIYTLIKIDEGSNLGAIRI
                     RIRSLKATIEKQAKNKKQLYPKYQPLKVPFTKEMRDQGYTILCPQMSPLHFQFVETAM
                     QESGYNLVVLPSVDKGAVDAGLKYVNNDACYPSILVTGQIMEALLSG"
BASE COUNT         3536 a         1227 c         2041 g         3338 t
ORIGIN      
        1 caaatgctag aattaacatg atcgtcccaa gcagtgaatt tacctgaaga caagctaatg
       61 caattgcaaa agcactgacc gtatgtcgtg aagggaaaga ttcaccttgc ttatgttcta
      121 ttaaaggatc aacagccata gcttcataag ggcgtttacg attaattact ttgcgcaaaa
      181 gtgttacaat taaaaatgaa gcaagtggct tataaagtgt tttagctaat aatgtatttt
      241 gagtataaag taaataaacg agcaaaattg aataaacaat aaaagtaata atagggatat
      301 agcgggtaaa gcccataatg attttttgca gcaagggatg tttacgaata ttagataaca
      361 ttgtttgata aaatttttcc ataaaaactc cttattttaa ttgaatgatc gtttcgcctg
      421 cattcattgt taagatgcgc cgaccagcac gtttatgttt atattgcttc cgaataaatt
      481 gttgtagtat tttacttgaa tagccgtgaa ctacagtaat ttctttcgta ttgggggata
      541 atgaatttag atagttatcc agccggattt tagcttcaat cgtagtacag ccgtgaatat
      601 cgatttgatt aaacataata gtctccttaa attaaagttt agttaattat actagatact
      661 ttaaaattaa acaaacttgc aatttacctg gatattaagt agaatataag agcatatgaa
      721 tggagtgata taaatggcac tgacagccgg aattgtagga ttgccaaatg ttgggaagtc
      781 aacattgttt aatgcaataa caaatgccca agttgaggca gcaaactatc cgtttgcaac
      841 aatcgaccct aatgttgggg ttgtagaagt acctgattat cgtttagata agttaacaga
      901 attagttgaa cctaaaaaaa ctgttccaac tactttcggt tttactgata ttgctggtct
      961 tgttaaaggg gcgagtcgtg gtgagggttt aggaaataaa tttttaggga atattcgtga
     1021 aactgatgca atttgtgaag ttgttcgctg ttttagagat aaggatgtaa ctcatgttga
     1081 tggcgatgtc gatcctatta gagatattga aacaattaat ttagagttga tttttgctga
     1141 tttagatact gtagaaaaaa gaattggacg aattggtaaa aaagcacaat caggggacaa
     1201 agaagcgaag ttagaagtcg ctattttaga aaaattaaaa agtacgctag aagccaataa
     1261 accagctcga gtgatcgagt ttagtaaaga agaaatggat gttgttaaac aatatacttt
     1321 attaacaatg aaaccaatca tttatgtggc aaatctaggt gaagaagact tagaagatcc
     1381 aactactaat ccacattata ataaagtcgt tgaatttgca gctggtgaag gggctgatgt
     1441 tgtccctatt tgtgcaaaaa ttgaaagtga attagttgga atggataaag aagaaaaaga
     1501 tttattttta caagatttag gaattgaaga aagcggtctt gataaattga tcaaagaagc
     1561 ctataaacta ttaggacttc gtacatactt tactgccgga gtacaagaag tacgagcttg
     1621 gacttttaaa gaaggtatga cagccccaga aatggcggga attattcata gtgatttcca
     1681 gagaggtttt attaaagcag aaacatatag ttttgatgat ttagtcgaat atggcagtga
     1741 acatgcttta aaagaagctg gtaagattcg tcaggaagga aaacaatatg ttggtcaaga
     1801 tggtgacatc atgttgttta aatttaatgt ctaatggcaa aaacaaaaaa gagttttatt
     1861 gagagaataa aaccgactgg agctaaagat ttaattagaa aaattatttt attagtttgt
     1921 atttgtgtat tttgttatag tgcgtataat ctagcctcaa tttttcttga atataagagc
     1981 atggatgata gtaataaaga agtagaagaa acatatgtta ctgaaaatac agaacaaaaa
     2041 aattcatata agacaattga ttttgaagcc ttattagcaa gaaattcaga tgttaaggga
     2101 tggatcgata ttcctgatac taaagttagt tatccaattg ttcaaggaga aactaatgat
     2161 acatatattc atagtgatat tgacaaaaag gaatttagag ccggttcaat ttttattgca
     2221 agtgaaaata aaaacccttt tactgattta aatactgtta tttatggtca taatatgaaa
     2281 aatggttcaa tgtttaataa cattaaatca tatacagaac aagattttgc agataaacac
     2341 ccgtatgttt atatttattt accagatggg acagtaagtc gttataaagt agtagcagca
     2401 catattattc cagaagagag tttattatat aatactggaa ttactgatat tcaagctttt
     2461 tatcaagaga tgttaaaaac tagtgatatt aaggttgatt ttgaacaagc tgcaggtaat
     2521 ccggtaatta ctttatcaac atgtacttca gctggtagtg aaagcggaaa acgtaatgtt
     2581 gttcatgcag tcttggatag agcaggaatt gatcctaaaa cggaaacaat ggattaattt
     2641 agacttgtgg aaaatcacaa gtctttttat atataatttt tgcaatataa acttttatag
     2701 taaattagtt atgaatggta cttgataaga agtagataat taacatattt ttctttttta
     2761 ttacatagat ttatagatta taatatctat atacgtccga atcaaagaaa aaaagggatt
     2821 aatttgacga ataaagttat tttatgtatg ataggtaaaa ctgttatagt tgggagcaag
     2881 aatatgaaag aaaaaatact caaatttatt acatcgaagt tttttgtctt aggaattcaa
     2941 ttattagcta cgattgcagt agtctacttt acattcaaac tggatttagt gccgactaag
     3001 tatttaattg ctggaattgt ggtattagcg ttgttattag caggattctt tggaattatt
     3061 tatagtagtg aacaaaaaat caaaaaagga ttatcgagta aacgaggaat agttactaaa
     3121 attattagtt tattgacaag tatcattttg attgctggct gtacttatat ttcaagaggg
     3181 aataatttta ttgaaaatgt ttctaatgcc actggacaag aatatgttgt ttcagtgatt
     3241 tcattaaaga atggtaaaat tactaaatta aaagatcttg atagttccaa aaaaattgga
     3301 gtatcttatg aaaaggatac tgttacgatt gctgaagcct taaaagatct tgataccgag
     3361 attggtgatc atgaatatac taagtatgat aattatgctt cacttgcaga tgctctttat
     3421 gagggaaaag tagatgcaat tgttgttggt gaacaatatc gaacgatgct gcagacaaat
     3481 catgaagaat ttaatgatgt tactaaggtt ttaaaatcat atgcttatga tgcgaaaatg
     3541 gaagtaacga ctaaacaaac agatgttact gaaaatgctt tttcaattta tgttacagga
     3601 attgatgttt atggaagttt aaaaacagta tcacgtagtg atgtaaattt aattgttacg
     3661 gtaaatccca aaacaaaaca aattttgatg acaagtatac ctcgtgattg tcaaattaac
     3721 ttgcataaaa atggaaaaat ggataaatta acacatactg gtatatatgg aacaagtgaa
     3781 acaattaata caattcaaga tttattggaa atgaaaatta attattttgc acgtactaat
     3841 tttagtggca tgactaatat tgttgatgct ttaggcggaa ttacagttaa ttcaagtgag
     3901 gcttttgaaa ctttgcatgg taattatcaa atcactgaag gtttgaatga gatggatggt
     3961 gataaagcat tgtgtttcgt tcgagaaaga aaaagattaa aacgtggtga ttttgctcgt
     4021 ggtgaaaatc agcaaaaggt tttaaaggca atgcttgata aagctatgtc acctaagatt
     4081 attactaatt ttaataatat tttaagtgca gttgaaggct gctttgaaac agatatgtca
     4141 gataaagaga ttaagtcatt gataaatatg caattaaatg atatggcgga ttggaagatc
     4201 atcaatgttc aaattgaagg tgagtatcaa ttaatggatg atactttttc aatgaaaggg
     4261 actaatagtg atgttatgat tccttttgaa tcacatattg aaagagtaca agaattgatc
     4321 aataaagtcg aagaaggtaa agaaattaaa gatagtgaat taaaaggatt aactcattaa
     4381 ataagaggag ctctttcgag ctcctttgtt gttttatttt tttataataa tggtataata
     4441 agagtattaa attggagata gaagaaaatg gtttttaatc gaaagtatta taaattatgt
     4501 tttattatat atatgtttat caatacttta gcattgggct atttaggtat tgagacgaac
     4561 tacctgtttg tgccattatt gatttgggca ttagtaataa ttgtgcatga tatttataaa
     4621 aaaaagttta aattaaaaaa gaattacagt ctgctaatga tcgttcaagg actgatttta
     4681 ttgcttgcta cagcgaggaa tgactatagt gatttaaatt cctatgttat tgcagtaatg
     4741 caattagtta tttatttatt gatttttaat aatcctgttt caatgagcaa agatgatatt
     4801 gaagatgaag tacgaatgat tattccacta gtaaatgtat tagttggtgg agcttcactt
     4861 atctctattg gaatgtattt agtgcatttt tctagtttag ctaacggctg gactttagga
     4921 atggtcggga atcgtttgtt tggaatttat tttaatagta atccagctgc ttttttagcg
     4981 tgtattacga ttgttttagc gttagtcgca gtaaggcaga agtttaaggg aaaatactgg
     5041 tatcttgcga atattgggat tcagctggta tatattcttt taacaagatg tcgtgcggca
     5101 ttaattattt tagcaatcat tatcgtcatg gttggatatt attttttaat acgtcgtaag
     5161 ccgtatagta attttaagag attagggctg gtaattagtc taattgtagt tattgctggt
     5221 gcaagtctag taggtcagcg ggtagttgag attgttccac agatgcaggg aattgcttct
     5281 aaggagacaa gtcgttttca aatggataaa gttgttaagg cgggacattt attaattgct
     5341 gggaattggc aagattttaa tcaaggattg acaattatcg atgaggtttc taatggacgg
     5401 gtatcattaa ctaaagctgc tttagaaatt tggcatacgg aaccagttat tggaattgga
     5461 gcaaataatt ttaaaaaaat aggcagtcag gaaacagatg cattggagta ttgggcagtt
     5521 caagttgttc atagtcataa tgttttttta gaggcgttag taactactgg ggtaattggg
     5581 tttatattat ttgttgtatt tttctttaag acacttttaa tgatttttaa tgttttgaaa
     5641 aaaagtcacg gcaaagaaat atactttatt gttcaaatgt tcgctatgat cgttttaagt
     5701 gaatttattg gtagtttatc tgattatggg gtattctata tttattcttt atcagctaca
     5761 ttagcatggt gctttttagg gtatttatac acatatcaaa atattacaga aataagtaat
     5821 attgataaga tttaaaggct attttggtag ccttttttaa ttgcgcaaga atggtgcaag
     5881 atgaaattaa ctaatgtgct atgcttagag caggggaagt aaataagaat gaacaatgaa
     5941 gagcaaatta ataaagtaat agattatatt gaagaacatc tacaggatga aaatttaaca
     6001 cttactacga ttgcacagga aattggctac ttaaaatatc atttgtatcg aatgtttact
     6061 atgattgcag gaattacaat ttatcaatag cttcaacgac gtcgttttaa tgaggcagcg
     6121 cggatgctta tttatgagga tattgcattg attagtggtt atgatactca acgttcattt
     6181 tctaaaagtt ttaaagcatt gtttaaaatg agtcctagca gttatcgaaa gcaacaaaag
     6241 ttattgctgg tgcaactaaa attaggactt aggacagttg ggttccaatt atttaactga
     6301 tgaacattgt actggcgggc cgatgttctt tttcttttga attatttagt tgataatatt
     6361 gtagattata aggggtaaaa ataaaaattg tttaaaaata aaagcataaa cgttgacatc
     6421 tttcgacatt ggtatataat gttagcaacc taacaatagg aggggaaata gtgaaagaaa
     6481 agcggatcgg aatagaaatt aggattcttg cgaacttgat ttgccggtgt ttaaatgaat
     6541 tgggttttaa tgaagagcac aatggtttaa ctggacctca agggttagtt ttagggtatt
     6601 tgtatgatca tcaagataaa gatatttttc aaaaagatat cgaagcaact ttcaatattc
     6661 gccgttcaac agcaacgggt ttattacaat gcttagaagg taatggtttt gtaaaacggg
     6721 taagtgttga ttatgacgca cgattaaaaa agatcgttct cacgaccaaa gcacatgagt
     6781 tcaaagagtt attagaatca catattcaaa aaatggaaga aattttggtt aaagatttag
     6841 aaccgcaaga ggtcgatgac ttaatcagaa taattggtaa gattaagaaa aatttagagt
     6901 aggagaacaa aatggaaaag aagtattaca tgggaattga cgttgggtca actacgataa
     6961 aaatttatat tacggatcag gatgataatt gtatctattc aaaatatgag cgacattatt
     7021 ctgatatcca agcaacaatt aaaacattaa tcaatgatgt taaagaacaa tttggtaatt
     7081 tagaagtaac ggctgtgatg acaggttcag gggggttatc actttcttca tgtctagatg
     7141 ttaaatttgt tcaagaagta attgcctgca caaaaacaat tgaaacttat attcctgaat
     7201 gtgatgttgc aattgaactt ggtggagagg atgctaaaat aacttatttt caaggttcac
     7261 tggaacaaag aatgaatgga acttgtgcag ggggaactgg agcttttatt gaccagatgg
     7321 catcattact tcaaactgat gctggaggat tgaatgaatt agcgaaaaat tatcaaatga
     7381 tttatccaat tgcttcacgt tgtggagtat ttgcgaaaac tgatgtacag cctctaatta
     7441 acgaaggggc aaacaaagag gatattgcga tttcaatttt tcaagcagta gtcaatcaga
     7501 cgatcagtgg tcttgcctgt ggtaaaccaa tccgtggtaa agtagctttt ttaggggggc
     7561 cgttatattt tttagatcaa ttaagacaac gttttattga aaccttaaat ttacaagatg
     7621 acgaaattat ttttcctgat aattctcaac tgtttgttgc catgggagct actctattag
     7681 cgaaagaaga agaaaagcca acaacgattg aagcgttgat tgagaaatta gataatattg
     7741 atgaaacaat gcttgctagc gaagatacac tagacccact atttgctgat gaaaatgatc
     7801 gaaatgggtt tagacatcgt cattatatta ataaagttcg aaaaaatgat attaaggggt
     7861 atgtcgggaa tgtgtattta ggtttagatg ttggttctac aacaactaaa gcggtattga
     7921 tcgatgataa tgactcttta ttatactctt tctatgattc aaatgaaggt aatccgctag
     7981 atgtggtagt aaaaatagtc aaagatattt atgattttat tcctcctaat gttaaattag
     8041 ttcgtagtgg agtcactggt tatggtgaag cattaatcaa ggcggctctt aaagttgata
     8101 tgagtgaagt ggaaacgatg gctcattata aggctgctac ttattttaaa gaagatgtaa
     8161 gtttcatttt agatattggt ggtcaagata tgaaagcaat caagatcaag gatggaatta
     8221 ttcaagatat tttattaaat gaggcttgtt caagtggatg tggttcattt attgagactt
     8281 ttgctaaatc cttaggatac gaggtgagtg attttgcaaa gctggcatta gagtcaaaag
     8341 cgccaatcga cttaggaagt cgttgtactg tatttatgaa ctcaaaagta aaacaagccc
     8401 aaaaagaagg ggcaaaagtt gaagatatta gtgctggttt atcatattca gtaatcaaaa
     8461 atgctctata taaagtaatt aaattaagaa gtaaagaaga actaggggac agtattttag
     8521 tccagggggg aactttttat aatgaggcag tattaagagc gttcgaaaaa gaagctggtg
     8581 ttaatgccat tcgtccagat attgcaggat tgatgggagc ttttgggatt gcccgtttag
     8641 cccgagaaag ttaccaagga gaaccgacaa ccttattaac taaggatgaa ttagaaaatt
     8701 tcacttgtca aagtgaaatt agatattgtg aaaaatgtac taaccactgt atgttgaccg
     8761 tatctacatt taatgataat agtgagtata ttagtggtaa ccgttgtgaa cgtggagcca
     8821 atataccagt ttcatcaaag aaattaccaa atttatttga ttacaaatat cgtcgtgtct
     8881 ttaactatcg ttcactaact gaagatcagg ctgtgcgtgg gactgtggga attcctcgag
     8941 tattgaatat gtatgaaaat tatccgtttt ggcatacttt ctttactaaa ttaggattta
     9001 gagtaattct ttctcctcgc tcatctaaag caatttatga aaagggcatt gaatcaattc
     9061 catcagaaag tgtttgttac ccagctaaat tagcacatgg gcatattgaa gctttgattg
     9121 agaaaggtat aaaatatatt ttctatccaa gtctagctta tgaacgtaaa gaatttgata
     9181 atgctaataa tcattataat tgtccggtcg ttacttcata tccagaagta attcgtaata
     9241 atgttgataa tctggagaat cgcagtatta tctatcgaaa tccatttatg gacctaagca
     9301 atcgaaaaac tttattcaat aacttaaagg atgaattgcg ggcatttaat gttaacgaca
     9361 aagaattatt gagtgcaatt aatgatgctt atgatgaatt agctaaatgt cggcaagata
     9421 ttcaagatca aggagaaatg gttttacaat atcttaaaga taataagatg acaggaattg
     9481 ttttatctgg acgtccatat catgttgatc cggaaatcaa ccatggatta gctgatttga
     9541 ttacagctga gggcatggca gttcttagtg aagatagtgt ttgccactta gataaggatt
     9601 tagatcaatt gcgagtagtc gatcaatgga catatcactc aagattgtat cgggctgctt
     9661 catttgtagc aactcagcct aatttagagt tgattcaatt gacttcattt ggttgtgggc
     9721 tagatgctgt gactagtgat caagttgctg atattttaaa agctcgtcat aaaatctata
     9781 ctttgattaa aattgatgag gggagcaatc ttggggcaat tagaatccga attcgttcat
     9841 taaaagcaac gattgaaaaa caagctaaaa acaaaaaaca gctctatcca aaatatcagc
     9901 ccttaaaagt accttttact aaagagatgc gtgatcaagg ttatactatt ttatgcccgc
     9961 agatgtcacc tttacatttc caatttgtag agacagcaat gcaggagtct ggttataatt
    10021 tagtggtttt accatcagtt gataaagggg cagttgatgc tggtttaaaa tatgttaata
    10081 atgatgcttg ttatccaagt attttagtta cgggtcaaat tatggaagct ttattgagtg
    10141 gt
//
LOCUS       DAOMTA010000013         9500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_55_length_9500_cov_2.506829, whole genome shotgun sequence.
ACCESSION   DAOMTA010000013 DAOMTA010000000
VERSION     DAOMTA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_9500_cov_2.506829"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /gene="leuA"
                     /locus_tag="PLK68_00845"
     CDS             <1..1660
                     /gene="leuA"
                     /locus_tag="PLK68_00845"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRM88986.1"
                     /translation="YKRFEPINFKERTWPDKQIEKAPTWVSVDLRDGNQALITPMKIE
                     EKVEMFQLLVDMGFKEIEVGFPSSSQIEYDFLRILIDENLIPDDVTVQVLTQAREHLI
                     RKTFESLKGLKRAIVHVYNSTSVLQRDVVFNRSKEEIKQIAVDGVKLVKELSREFDGE
                     VILEYSPESFTGTELEYALEVCEAVMDEWGATKDNKVIINLPSTVEMATPNVYADQIE
                     WMCTHFSDRDRVIVSLHTHNDRGCGVAATELGIMAGADRVEGTLFGNGERTGNLDIVV
                     VALNMFTQGIDPKLDMSNINNIKHVYEKVTKMEIPPRQPYVGKLVFTAFSGSHQDAIN
                     KGTHAMHERDSQIWEVPYLPIDPSDLGRQYEPIVRINSQSGKGGVAYVMESMYGFHLP
                     KGLQVDFAKVIQDISEEEGEVSPERVYDTFIEEYVDIDEPYRFIKQKLIDISEDDSEF
                     ERRAEITIEAHGEVTTLTGYGNGPIDAVKNALNSLPEMHSHLLDYSEHALTSGSSSKA
                     AAYVYLRAKGSARQEYGVGIHPNITTATVKAIISGMNRLYKTLK"
     gene            1678..2187
                     /gene="ilvN"
                     /locus_tag="PLK68_00850"
     CDS             1678..2187
                     /gene="ilvN"
                     /locus_tag="PLK68_00850"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRM88987.1"
                     /translation="MNRLVLSLLVENNPGVLSRVAGLFSRRGYGIESLSVGKTNEPNV
                     SRMTVVAVGDELILNQIEKQLGKLVEVIEIFPLKPEESVYRELVLVKVEADEKQRSSL
                     VSIADIFRARIIDVAPASLVIEVTGDQSKIDGLLAMLEGFNVVEMVRTGLSGIKRGLG
                     EIDIESHNK"
     gene            2208..3224
                     /gene="ilvC"
                     /locus_tag="PLK68_00855"
     CDS             2208..3224
                     /gene="ilvC"
                     /locus_tag="PLK68_00855"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRM88988.1"
                     /translation="MAKIFYESDCDLSLLDGKTVAVIGYGSQGHAHALNMKESGVNVI
                     IGLYEGSKSWKKAEEQGFEVYTSAEAAKKADIIMILINDELQAKLYKESIEPNLEEGN
                     MLMFAHGFNIHFGQIVPPKNVDVTMVAPKGPGHTVRSEYQVGKGVPCLVAVEQDATGR
                     AQDVALAYALAIGGARAGVLETDFRTETETDLFGEQAVLCGGVCALMQAGFETLVEAG
                     YDPRNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIITEDTKKAMK
                     KVLQDIQDGTFAKDFLLDMSEAGGQAHFKAMRKLASEHISEKTGEDVRKLYAWSDEDK
                     LINN"
     assembly_gap    3369..3378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3379..4571
                     /gene="leuC"
                     /locus_tag="PLK68_00860"
     CDS             <3379..4571
                     /gene="leuC"
                     /locus_tag="PLK68_00860"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRM88989.1"
                     /translation="KLDVVMANDITGPMALPIFHQMADKVFDKDKVILVPDHFTPNKD
                     IKSAENSKAILDFSNDQCLTHHMEQGKCGVEHAILPEKGIVVAGECIIGADSHTCTYG
                     ALGAFSTGVGTTDIAGMATGELWFKVPSAIKFVLTGKPSQFVSGKDIILDIITRIGVD
                     GARYKSMEFVGEGIQHLTMDDRFTICNMAIEAGAKNGIFPVDEQTIAYMEKHSVKEYT
                     VYEADEDAEYDEVIEIDLGKVRPTVAFPHLPGNGHTIDEIEAMDKIMIDQVVIGSCTN
                     GRLSDLRKAAAILKGKQVAKNVRVMVVPATQKIFVQCIHEGLAEIFVEAGCAFNTPSC
                     GPCMGGHMGVMAKGEKCVSTTNRNFVGRMGDTEALIYLASPEVAAASAIAGYIANPEK
                     VGEE"
     gene            4571..5050
                     /gene="leuD"
                     /locus_tag="PLK68_00865"
     CDS             4571..5050
                     /gene="leuD"
                     /locus_tag="PLK68_00865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRM88990.1"
                     /translation="MKIYKYGDNVDTDVIIPARYLNSFDAKELASHAMVDIDPDFAKT
                     VESGDIIVAGKNFGCGSSREHAPLCLKTAGTKCVIAESFARIFYRNSINIGFPIMECP
                     EAVAGINQGDEVTVDFTTGLITNITKGETYQSQPFPEFLQKMIELDGLVNYVNSKKG"
     gene            5056..6138
                     /gene="leuB"
                     /locus_tag="PLK68_00870"
     CDS             5056..6138
                     /gene="leuB"
                     /locus_tag="PLK68_00870"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRM88991.1"
                     /translation="MEKNIAVIKGDGIGPEIVTEAMKVLDAIADKYNHKFNYTQILMG
                     GASIDVHGVPLTDEALAIAKNSDSVLLGSIGGDTTTSPWYKLEPNLRPEAGLLKIRKE
                     LGLFANLRPAVLYDELKGACPLKEELTEGGFDMMIMRELTGGLYFGERSTKEENGELV
                     ARDAMSYSETEIRRIAKRAFDIAMKRNKKVTSVDKANVIDTSRLWRKVVSEVAKDYPE
                     VVLEHMLVDNCAMQLVKDPKQFDVILTENMFGDILSDEASMVTGSIGMLASASLRVDK
                     FGMYEPSHGSAPDIAGKNIANPIATILSAAMMLRYSFDLDDEAKVIENAIEKVLQQGY
                     RTSDIMSDNKTLVGTKEMGDLIVANL"
     gene            6151..7824
                     /gene="ilvD"
                     /locus_tag="PLK68_00875"
     CDS             6151..7824
                     /gene="ilvD"
                     /locus_tag="PLK68_00875"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM88992.1"
                     /translation="MKSDNVKKGMQQAPHRSLFNALGLTEEEMDKPLIGIVSSYNEIV
                     PGHMNLDKIVEAVKMGVALAGGVPREFPAIAVCDGIAMGHIGMKYSLVTRDLIADSTE
                     AMALAHQFDALVMVPNCDKNVPGLLMAAARINIPTIFVSGGPMLAGRVHGKKTSLSSM
                     FEAVGSYSAGKIDEEEVRYYENHACPTCGSCSGMYTANSMNCLTEVLGMGLRGNGTIP
                     AVYSERIKLAKHAGMQIMELLKKDIRPRDIITKESMMNALTMDMALGCSTNSMLHLPA
                     IAHEIGFDFNIKFANEISEKTPNLCHLAPAGPTYMEDLNEAGGIYAVMKEISKLNLLN
                     LDCMTVTGKTVGENIKDAVNFDPEVIRTIENPYSKTGGLAVLSGNLAPDGSVVKRSAV
                     VPEMMEHSGPARVFDCEEDAIEAIKGGKIVAGDVVVIRYEGPKGGPGMREMLNPTSAI
                     AGMGLGSTVALITDGRFSGASRGASIGHVSPEAAVGGPIALVEEGDIIEINIPEYKIN
                     LKISDEEMAKRKAKWQPREPKVTTGYLARYAAMVTSGDRGAILEVQKNK"
     gene            7839..>9500
                     /gene="ilvB"
                     /locus_tag="PLK68_00880"
     CDS             7839..>9500
                     /gene="ilvB"
                     /locus_tag="PLK68_00880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRM88993.1"
                     /translation="MLLTGSQIVAECLIEQGVDTVFGYPGGTILNIYDALYQYQDKIH
                     HILTSHEQGAAHAADGYARSTGKVGVCMATSGPGATNLVTGIATAYMDSTPLVAITAN
                     VAVSLLGRDSFQEIDIAGVTMPITKHNFIVKDIKDLAPTIRKAFHIASEGRPGPVLVD
                     ITKDVTAAKFDFEPIVPEVIEPRKYTYGAQELDQAIKMIEASEKPFIFVGGGAIASDA
                     ASELKEFAEKIDAPVTDSLMGKGAYDNTRPRYTGMLGMHGTKASNFGVSQCDLLIVVG
                     ARFSDRVTGDTNRFAKDAKIIHIDVDAAEINKNVVVDLGIVGDAKNVLKELNEKLTRQ
                     NHPQWLKEIRDLKERYPLSYDDEGLTGPYVIEQIDYLTNSEAIICTDVGQHQMWAAQY
                     FNYRRPRQFISSGGAGTMGFGLGAAMGAKLANPHQTVFNIAGDGCFRMNLNELATLSR
                     YNIPVIQVVMNNQVLGMVRQWQTLFYGQRYSNTILEDKVDFCKVAEGLGCKAIKVTTK
                     EEVASAIKIAMEHDGPVVIECMIGKDDKVFPMVAPGGAIAEAFEDT"
BASE COUNT         3146 a         1303 c         2148 g         2893 t
ORIGIN      
        1 gtataagaga tttgaaccaa tcaactttaa agaacgtact tggccggata aacaaattga
       61 aaaagcgcca acttgggtct ctgtagattt acgtgatggc aatcaggcgt tgattacccc
      121 tatgaaaatc gaagaaaaag tcgaaatgtt tcaactttta gttgatatgg ggtttaaaga
      181 aatagaagtt ggttttcctt cttctagtca aattgaatat gattttttaa gaattttgat
      241 tgatgaaaat ttaattcctg atgatgtgac tgttcaagtt ctaactcaag cacgtgaaca
      301 tttaattaga aagacttttg aatcattaaa aggattaaag agagcaatcg ttcatgttta
      361 taattcaact tcagtattac aacgtgatgt cgtctttaat cgaagtaaag aagaaattaa
      421 acagattgca gtcgatggtg ttaaattagt caaagaatta agtagagaat ttgatgggga
      481 agtaattctt gaatattcac cagaaagttt tactggaact gaactggaat atgccttaga
      541 agtttgtgaa gcggtgatgg atgaatgggg cgctactaaa gataataaag taattatcaa
      601 tttaccgtca acagtagaaa tggcaacacc taatgtgtat gctgatcaaa ttgaatggat
      661 gtgtactcac tttagtgatc gtgatcgtgt cattgtatcg ttgcatactc acaatgatcg
      721 tggttgtggt gtagcggcta ctgaattagg aattatggca ggagctgatc gtgttgaagg
      781 aacactattt ggtaatggtg aaagaacagg aaatcttgat attgtcgtag tagccttaaa
      841 tatgtttacc caaggaattg atccaaaact agatatgagc aatatcaata atataaaaca
      901 tgtttatgaa aaagtaacga aaatggaaat cccacctcgt caaccatatg taggaaagtt
      961 agtgtttacc gcattttcag gtagtcacca agatgcaatt aataaaggaa ctcatgcaat
     1021 gcatgaacgt gacagtcaaa tttgggaagt accttatttg ccgattgatc ctagtgatct
     1081 aggccgccaa tatgaaccga ttgtgcgtat taattcacaa tctggtaaag gtggtgtagc
     1141 atatgtaatg gaaagtatgt atggtttcca tttaccaaaa ggattacaag tagattttgc
     1201 taaagtaatt caagatataa gtgaagaaga aggagaagtt tctccagaaa gagtctacga
     1261 tacattcatt gaagaatatg tagatattga tgaaccttat cgctttatta aacaaaagct
     1321 gattgatata agtgaagatg atagtgagtt tgaacgtcgt gctgagatta cgattgaggc
     1381 gcatggtgaa gttacaactt taacaggata tggtaatgga ccaatcgatg ctgttaaaaa
     1441 tgctttaaac tcacttccag aaatgcattc acacttatta gattatagtg aacatgcatt
     1501 aacttcagga tcaagctcaa aagcagcggc ttatgtttat ttaagagcta agggaagtgc
     1561 tagacaagaa tacggagtgg ggattcatcc aaatattaca actgcaactg taaaagcgat
     1621 aatatctgga atgaatcgtc tgtataagac gctaaaataa gtggaaagga gccgaacatg
     1681 aatcgtttag tattatcgtt attagtggaa aataacccag gtgtattaag cagagtggct
     1741 ggtttattta gtcgtcgtgg ttatggaatt gaaagtttaa gcgttggcaa gaccaatgaa
     1801 cctaatgttt cacgcatgac ggttgtggct gttggtgatg aattgatttt aaatcaaatt
     1861 gaaaaacagt taggtaaact agtcgaagta attgaaattt tcccattgaa accggaagaa
     1921 tcggtatacc gggaattagt tttagttaaa gttgaggctg atgaaaaaca acgttcgtca
     1981 cttgtttcca ttgccgatat ctttagagca cggattattg atgttgcacc agcatcatta
     2041 gtaatcgagg taacaggaga tcagtcaaag attgatggac tgcttgccat gttagaaggt
     2101 ttcaatgttg ttgagatggt tagaaccgga ttatctggta tcaaacgtgg tcttggcgaa
     2161 attgatatag aaagtcataa caaatagaaa tagggggatt agaaaaaatg gctaaaattt
     2221 tttatgaatc agattgtgat ttatctttat tagacggtaa aactgttgcc gtgatcgggt
     2281 atggttctca aggacatgct catgcattaa atatgaaaga atcaggagta aacgtaatta
     2341 tcggtttata tgaaggttct aaatcttgga aaaaagctga agaacaaggt tttgaagttt
     2401 atacttctgc tgaagcagct aaaaaagctg acatcatcat gattttgatc aatgatgaat
     2461 tacaagctaa attatacaaa gaatcaattg aaccaaactt agaagaagga aatatgttaa
     2521 tgtttgctca tggtttcaat attcattttg gacaaatcgt accacctaag aatgttgatg
     2581 taactatggt tgcaccaaaa ggacctggac atacagtacg tagtgagtac caagttggta
     2641 aaggtgtacc ttgcttagtc gctgttgaac aagatgctac tggtcgtgct caagatgttg
     2701 ctttagcata tgctttagct atcggtggag caagagctgg agttcttgaa actgatttta
     2761 gaacagaaac agaaactgat ttatttggtg aacaagcagt attatgtggt ggagtttgtg
     2821 ctttaatgca agctggtttt gaaacattag ttgaagctgg gtatgatcca agaaatgctt
     2881 atttcgaatg tatccatgaa atgaaattaa tcgttgattt aatttaccaa tcaggttttg
     2941 caggaatgag atattcaatt tcaaatactg ctgaatatgg tgattatatt acaggaccaa
     3001 aaattatcac agaagatact aaaaaagcaa tgaaaaaggt attacaagat attcaagatg
     3061 gtacatttgc taaagacttc ttattagata tgtctgaagc tggtggacaa gctcatttca
     3121 aagcaatgag aaaattagca agcgaacata tttctgaaaa aactggggaa gatgtaagaa
     3181 aattatacgc ttggtctgat gaagataaat taatcaataa ctaatttatt ataacaacac
     3241 acttttgtgt gttgtaatta aggagtaatt tcttactccc taattacaac ctacaaaggt
     3301 aaagataagg gggaatataa aagtggcaat gacaatgaca caaaaaatat tagcaaaaca
     3361 tgcaggatnn nnnnnnnnca aaacttgatg ttgtaatggc taacgacatc acaggaccga
     3421 tggcattacc aatttttcat caaatggctg acaaagtctt tgataaagat aaggtgattt
     3481 tagttcctga tcattttaca ccaaataaag atattaagtc ggctgaaaac tctaaagcaa
     3541 ttttagattt ttcaaatgat caatgtttaa ctcatcatat ggaacaaggt aaatgtgggg
     3601 ttgaacatgc aattttacca gaaaaaggaa ttgttgtcgc tggtgaatgt atcattgggg
     3661 ctgattcaca tacatgtact tatggcgctt taggagcttt ttcaactggt gttggaacta
     3721 ctgatattgc aggaatggca actggtgaat tatggtttaa agtgcctagt gcaatcaaat
     3781 ttgtcttaac cggaaagcct agtcaatttg ttagtggtaa agatattatt ttggatatta
     3841 ttactcgtat tggagttgat ggggcccgtt ataaaagtat ggaatttgtt ggtgaaggaa
     3901 ttcaacactt aacaatggat gatcgtttta cgatttgtaa tatggcaatt gaagctgggg
     3961 ctaaaaacgg gatttttcct gttgatgagc aaacgattgc ttatatggaa aaacactcag
     4021 tgaaagaata tactgtctat gaagctgatg aggatgctga atatgatgaa gtgattgaaa
     4081 tagatttagg taaagttaga ccaacagtag ctttcccgca tttaccaggg aatgggcata
     4141 cgattgatga aattgaagca atggataaga ttatgatcga tcaagtcgta attggttcat
     4201 gtacgaatgg gcgtttgtct gatttacgta aagcggcagc aatccttaag ggaaaacaag
     4261 tagctaaaaa tgttcgtgta atggttgttc cagcgactca gaaaatcttt gttcaatgta
     4321 ttcatgaagg tttagcagaa atttttgttg aagctggatg tgcatttaat accccaagct
     4381 gtggaccttg tatgggtgga catatgggag ttatggctaa aggtgaaaag tgtgtttcta
     4441 caactaaccg caattttgtt ggtcgaatgg gcgacacaga agcgttaatt tacttagctt
     4501 ctccagaagt agcagccgca agtgcaattg cgggatatat tgcaaatcct gaaaaagtag
     4561 gtgaagaata atgaaaatat ataaatatgg tgataatgtt gatacggatg taattattcc
     4621 agcacgttat ttaaattcat ttgatgctaa agaattagct agtcatgcga tggtggatat
     4681 tgatcctgat tttgctaaaa cggttgaatc gggtgatatt attgttgctg gaaaaaattt
     4741 tggttgcggt tcttcaagag aacatgctcc tctatgttta aaaacagcag gaactaaatg
     4801 cgtcattgca gaaagttttg ctcgtatttt ctatcgtaat tcaattaata ttggatttcc
     4861 aatcatggaa tgtccagaag cagtagcagg aattaatcaa ggtgatgaag ttacagtaga
     4921 ttttaccact ggtttaatta ctaatattac aaaaggtgaa acttatcaaa gtcaaccttt
     4981 cccagagttc ttacaaaaga tgattgaatt agatggttta gtcaactatg ttaatagtaa
     5041 aaaggggtaa ggataatgga aaaaaatatt gctgtaataa aaggtgatgg aatcggaccg
     5101 gaaattgtaa ctgaagcgat gaaagtttta gatgctatcg ctgataaata taatcataaa
     5161 tttaattata ctcaaatttt aatgggtggt gcttcaatcg atgttcatgg cgttcctcta
     5221 actgatgagg cgttggcgat tgctaaaaat agtgatagtg ttttacttgg gtcaattggt
     5281 ggtgatacca caacatcacc ttggtataaa ttagaaccta atcttcgtcc tgaagcgggg
     5341 ttgttgaaaa ttcgtaaaga gttaggattg tttgctaatt taagaccggc ggtcttatat
     5401 gatgaattaa agggtgcttg tcctttgaag gaagaattaa ctgagggtgg gtttgacatg
     5461 atgatcatgc gtgaacttac cggcgggtta tattttggtg aacgttcaac gaaagaagaa
     5521 aatggtgaat tagtggctcg agatgcaatg agttatagtg agactgaaat tagaagaatt
     5581 gctaaaagag cgtttgatat tgctatgaaa cgtaataaaa aagtcacgag tgttgataaa
     5641 gctaatgtta ttgatacttc aagattatgg cgtaaagttg taagtgaagt tgcaaaagat
     5701 tatcctgaag ttgtgctgga gcatatgtta gttgataact gtgcaatgca actggtaaag
     5761 gatccaaaac aatttgatgt tattttgacg gagaatatgt ttggggatat tctaagtgat
     5821 gaggctagta tggtaacagg ttcgattggg atgctggcaa gtgcatcact acgtgttgat
     5881 aaatttggaa tgtacgaacc aagtcatggt agtgcgcctg atattgctgg taaaaatatt
     5941 gctaatccaa ttgctactat tttatcagct gcaatgatgc ttcgttattc gtttgatcta
     6001 gatgatgaag cgaaagtgat tgaaaatgca attgaaaaag tattacaaca aggatatcga
     6061 acaagtgata tcatgtcaga taataaaact ttagtaggaa ctaaagaaat gggtgactta
     6121 attgttgcaa atttataagg gggatagaaa atgaaaagtg ataatgtaaa aaaagggatg
     6181 cagcaggcac cacatcgatc attatttaat gctttaggtt taactgaaga agagatggat
     6241 aaaccgttaa ttggaattgt tagttcatat aatgaaattg tccctggaca tatgaatctt
     6301 gataaaatcg ttgaagcagt aaaaatggga gtagccctag cgggtggagt acctagagaa
     6361 ttcccggcta ttgcggtatg tgatggaatt gctatgggac atattggaat gaagtattcg
     6421 ttagtaacta gagatttaat tgctgatagt actgaagcaa tggctttagc tcatcaattt
     6481 gatgctttag ttatggtacc caactgcgat aaaaatgtac caggattatt gatggcggct
     6541 gcaaggatca atataccaac tatctttgtt tctgggggac caatgttagc tggacgcgta
     6601 catggtaaaa aaactagttt atcatcaatg tttgaagcgg taggaagcta tagtgcaggt
     6661 aaaattgatg aggaagaagt tcgttattat gaaaatcatg cttgtccaac atgtggttca
     6721 tgttctggga tgtatacggc taattcaatg aactgtctga ctgaagtgtt aggaatgggg
     6781 cttcgtggga atgggactat tcctgctgtc tattctgaaa gaattaaatt agctaaacat
     6841 gcgggaatgc aaattatgga attgttaaag aaagacattc gtcctcgtga tattatcact
     6901 aaagaatcaa tgatgaatgc tttaacgatg gatatggcgt tgggatgttc aactaattca
     6961 atgttacatt tgccagctat tgctcatgaa attggttttg atttcaatat aaagtttgct
     7021 aatgaaataa gtgaaaaaac acctaatctt tgccatttgg cgccagctgg acctacttat
     7081 atggaagact tgaacgaagc aggcggtatt tatgcagtaa tgaaagaaat cagtaaatta
     7141 aatttattga atcttgattg tatgactgtt actggtaaaa cagtaggtga aaatattaaa
     7201 gatgcagtta actttgatcc tgaagtaatt cgaacaattg aaaatccata tagtaaaaca
     7261 ggcggattag ctgttttatc gggaaattta gcgcctgatg gtagtgtagt aaaacgctca
     7321 gcagttgtgc cggaaatgat ggaacatagt ggtcctgcaa gggtctttga ttgtgaagaa
     7381 gatgcgattg aagcaattaa aggtggtaag atcgtagcgg gagacgttgt tgtgattcgt
     7441 tacgaaggac ctaagggtgg gcctggaatg cgcgaaatgt tgaatccaac ctctgcaatt
     7501 gccggaatgg gcttaggatc aacagtagcc ttaattactg atggccgatt ctcaggggca
     7561 tctcgtggag cttcgattgg acatgtttca cctgaagcgg cagtaggtgg accgattgct
     7621 ttagttgaag aaggcgatat tatagaaatt aatattcctg aatataaaat caatttaaaa
     7681 atttctgatg aagaaatggc aaaacgtaaa gctaaatggc agccaagaga accaaaagta
     7741 acgactggtt acctagcgag atatgcggca atggtaactt caggtgatcg tggtgctatt
     7801 ttagaagtac aaaaaaataa gtagagggag gtatttaaat gttattaaca ggttcccaaa
     7861 ttgttgctga atgtttaatt gaacaaggtg ttgatacagt gtttggttat ccaggtggaa
     7921 caattctaaa tatttatgat gctttatacc aatatcaaga taagattcat catatcttaa
     7981 catctcacga acaaggtgcg gcccatgctg ctgacggcta tgcgcgctca acaggaaagg
     8041 ttggagtgtg tatggcgact tctgggccgg gagctacgaa tctggttaca gggattgcta
     8101 cggcttacat ggattcaaca ccattagttg caattactgc taatgttgca gtatcgctgc
     8161 ttgggcgtga cagcttccaa gaaattgata tcgctggagt tactatgcca attactaaac
     8221 ataattttat tgttaaagat attaaagatt tggcaccaac gatccgtaaa gcctttcata
     8281 ttgcgagcga aggtcgtcct gggccggtat tagtggatat aactaaggat gttacagcgg
     8341 ctaaatttga ttttgagcca attgttccag aagtgatcga accacgtaaa tatacttatg
     8401 gtgctcaaga attagatcaa gctatcaaga tgattgaagc aagtgaaaaa ccatttattt
     8461 ttgttggtgg tggggcaatc gctagtgatg cggccagcga attgaaagaa tttgcagaaa
     8521 aaattgatgc tcctgttacg gattctttaa tgggtaaagg cgcatatgat aatacccgtc
     8581 ctcgttatac tggaatgtta gggatgcatg gaacaaaagc ctcaaatttt ggggttagtc
     8641 aatgtgattt attgattgtt gtaggggctc ggttctctga tcgagttact ggagatacaa
     8701 atcgttttgc taaagatgca aagattattc atattgatgt tgatgctgct gaaattaata
     8761 aaaatgttgt tgttgattta gggatcgttg gtgatgctaa aaatgtactt aaagaactta
     8821 atgaaaaatt aacacgtcaa aatcatcctc aatggttaaa agaaattcgt gatttaaaag
     8881 aacgttatcc tttaagttat gatgatgaag gtttaacagg tccttatgta attgaacaaa
     8941 ttgattatct cactaatagt gaagcaataa tttgtactga tgttggacaa catcaaatgt
     9001 gggcggctca atactttaat tatcgtcgcc ctcgtcaatt tatttcttca ggtggtgctg
     9061 gaaccatggg atttggttta ggagcagcaa tgggagcaaa attagctaat ccccaccaaa
     9121 ctgtctttaa tattgctgga gacggatgtt ttagaatgaa tttaaatgaa ttagcaactc
     9181 taagccgtta taatattcca gtaattcaag tcgttatgaa taatcaagtt ttaggaatgg
     9241 ttcgtcaatg gcaaacattg ttctatggac aacgttattc caacacaatt ctcgaagata
     9301 aagttgattt ttgtaaagtt gctgaaggtt taggttgtaa agcaattaaa gttacaacta
     9361 aagaagaagt tgcttcagca atcaaaattg caatggagca tgacggtcct gtagtgattg
     9421 aatgtatgat tggtaaagat gataaagtat tcccgatggt tgctccaggt ggtgcaattg
     9481 cggaagcttt tgaggatact
//
LOCUS       DAOMTA010000014         9248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_61_length_9248_cov_2.788861, whole genome shotgun sequence.
ACCESSION   DAOMTA010000014 DAOMTA010000000
VERSION     DAOMTA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61_length_9248_cov_2.788861"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..805)
                     /locus_tag="PLK68_00885"
     CDS             complement(23..805)
                     /locus_tag="PLK68_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM88994.1"
                     /translation="MIDLKNAKRLFDEYVANYDKDNPKVALKIEHTYRVMEASKNVAV
                     SLGLDQDEIDLASLIGLLHDIGRFEQLKRYNCFIDSKTIDHALLGVQILFDDNLISKF
                     DIDQKDYPLIYKAIFNHNKYKIAEGLSEHEMLHCKIIRDADKIDIFKTGLLETFEAFL
                     DAGQEILENDIITESIYETFMNSESILSTTRKTDLDRWVSFLALIFDLNYQYSCNYVY
                     QQDYITKLVKRLNYQNSDTFKKMLNIMNHANEYLKEKSDLNN"
     gene            922..1416
                     /locus_tag="PLK68_00890"
     CDS             922..1416
                     /locus_tag="PLK68_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM88995.1"
                     /translation="MKLRLTTTNDLTAVMTIINQAKVYFKEQGINQWQDGYPDELTII
                     NDISRHEAYVLEDNGEIVATAMISKELEPSYNYIEGKWLQDNAYIVVHRIAIRNDQKG
                     KGLAKIIIDEGLKLYPKKPSIRMDTHDDNLSMQRFLIKYGFEYCGTIYLENKETRRAY
                     EKIL"
     gene            complement(1439..2248)
                     /locus_tag="PLK68_00895"
     CDS             complement(1439..2248)
                     /locus_tag="PLK68_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM88996.1"
                     /translation="MFYLKSSDNAKLAVEDINPAHQKTVVLVHGWPICQEMYEYQKGI
                     LNDCRYRIISYDIRGLGMSQVMGRGYDYDQLAIDLHSVLITLNVHNVTLVGFSMGGAI
                     CVRYMSKFGKERVSKLVLAGAAVPSYTRTIHNPEGQSIDAVNQLIEQCYTNRPKMVHD
                     FGQNVFALNHGSEFMNWFTSICLKGSGIGTIQTAISLRDEDVYQDLFEIKVTTLIMHG
                     VLDKICPFSFALIMHECIEDSILCKFEYSGHGIFYDERERFNQVLIDFIEQ"
     gene            2838..3338
                     /locus_tag="PLK68_00900"
     CDS             2838..3338
                     /locus_tag="PLK68_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRM88997.1"
                     /translation="MDTKVNKPYPEIKVTVPNETYGLMILDNVGGMNSETSAICQYIY
                     NHSIAGEDFLELKKTFLNISMVEMHHLDIFMTLALELGLDPRWWSCLNDQCTYWSPSY
                     LNYPNSLEEVLKTAIDAEYQAIDKYMYQASVIKDPYIVAILNRIIEDEELHIKVLKKW
                     ETKLVR"
     gene            complement(3340..>3702)
                     /locus_tag="PLK68_00905"
     CDS             complement(3340..>3702)
                     /locus_tag="PLK68_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM88998.1"
                     /translation="LGDTVRFSYVFLTSESSNPLRPYRNVGKITRIVKNTRNPYLIGN
                     DQGWVNDQVIEGRVSYLSNPNYVGDSLVGALQQINVDTSFENRQHLAKLNGITNYVGS
                     AAQNLQLLQLLKEGKLIS"
     assembly_gap    3703..3712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3713..4319)
                     /locus_tag="PLK68_00910"
     CDS             complement(<3713..4319)
                     /locus_tag="PLK68_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRM88999.1"
                     /translation="MTTHGIDVSQYQGVIDWEIVQDRVDFAILRCGFGQDRTDQDDRL
                     FKRNADECTRLGIPFGVYLYSYAKNSSDARGEAQHVLRLVKNYKMAYPVYYDLEDNNT
                     TGKQSNDVIANIAKTFADELEANGYYVGMYASLYWWNTKLTDPIFDNYTRWVANYAAE
                     LNYDKPYDMWQYSSTGWVQGVPTIVDMNYCYADFPAIIKRAG"
     gene            4502..5704
                     /locus_tag="PLK68_00915"
     CDS             4502..5704
                     /locus_tag="PLK68_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM89000.1"
                     /translation="MKKVFVTALGVCTMLSLVTIPTPVQATDFSGQEDKYMKLCSSSN
                     LSTNNLNTCKEFNTYLKNKNKELKSQVSDSKSKVSDTQNSLNSISSEISALNDQIAEK
                     QKEIEYLQTSISNLEASIAKKEEEVKERMYSMQSYNNNNSYIDFIFGASSFTDMFARI
                     DSVNEITSYDDELVAQLADEKEQVETQKATVVTAKANIESQKSSKQALQQEYQALFEK
                     QNADLIAQEKAAAQAADSSQKITDNIAALLAATEKSQVSGGSVVSGDSAVGNAIAQKA
                     LTRVGYMYVWGGCHSMSEIANPNHTAFDCSGLVNWAYYQSGVNIGSNNTKSLASKGVS
                     VSRNNMQAGDIILFSSNGSTSGIHHVGIYIGGGNMVHAPQTGKPVQVADLGYSYWQKE
                     WYDVRRLY"
     gene            5895..7217
                     /locus_tag="PLK68_00920"
     CDS             5895..7217
                     /locus_tag="PLK68_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM89001.1"
                     /translation="MKIKKVISTALTLTIVTSACYYAAPVAADEFDGNESKYMTLCSS
                     SNLSSSNKSTCEAFNKYLKTKNSELTKKLAEQKEAASDTKTTLESVQKQLDEVNKQIS
                     DKEAEIEYLNTTIANLQASIEKNTQLLKDRMYAMQSYANENTYINFIFGASNFTEMFS
                     RIDGYNELTQSDKELIEELNKQRKEVEQQQTILEEAKATLVIQQEEQKTLKTKYTALL
                     EEQNKSIASTQNTIYDYGEMTETLDAAIKKFNEEAYETPTPTPPPPKPDNGGNNNNSG
                     GNNNNNNNNSDSGNNNNGGNNNNDSSTSDNLGEKIYAAAYAKLGSRYWWGAQGPTYFD
                     CSGLVYWSLKQAGVSGGRDTAAGYSRKWSAVSFANSKTGDLVCFGSPAYHIGIIVVNS
                     DGSRSMVHAGGGDSSTHGDNPKAYVKVSSIEPGSYYYSRISTIRRVSK"
     gene            7382..8665
                     /locus_tag="PLK68_00925"
     CDS             7382..8665
                     /locus_tag="PLK68_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM89002.1"
                     /translation="MKAKIISLFHYLLVFLLIIFNTSCQAKSINIVDTNNNDQIELNF
                     YGYKTEAINVVAIEEILQAYMDKNPNITITYESVKGTEYYEILKKRLASNNYDDIFMI
                     DEDNLQQLTNYNYFEDLSKLKTIKNFNTNSLEQMVQPDNTIPYIPTSISAFGLYCNLD
                     LLEKHHQSVPKNNQEFMAVCQYFIEQGITPIIANNDISLKTIALAKGLYPIYQSPEKD
                     RIIASLNQDPSILCNYLKEGYQYVKQLIDNKFIDAATTLKTEKTADDLIQFEENKNPF
                     MLTGAWASVRVEKSSPDLNFKVYPYPILDDGAILVSNIDTRVCINAKGPHITEAKKFI
                     EYLSQEDVMWKFVNSQSSFSPLKEQRIADDPKIQELSPYLTSEKTILGSDSNINLPLW
                     SLTHDGITQLLTGNSIDSALEIIKNYEQEDDSNEK"
     gene            8655..>9248
                     /locus_tag="PLK68_00930"
     CDS             8655..>9248
                     /locus_tag="PLK68_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF-domain containing protein"
                     /protein_id="HRM89003.1"
                     /translation="MKNKKISLMIVGGISAVIFVVFSMFIYVNYVQDSLWDKSINDIL
                     ETTSQEEKAFNVYMTKEMTNSQNILTNIAYLQTKIPQYLQHLSNREEAKYLYIDYTND
                     CYYDENGNYPLGDEQLFQQIKADNNSQGFLEPYLNSKTGIKTFATYVKTSDSQSVIVK
                     ETQLSFIIENFSLSFYNDLGFSYIVKDNGEILIRSKHR"
BASE COUNT         3325 a         1523 c         1355 g         3035 t
ORIGIN      
        1 aacttacctc aaaaaagtga tcttaattat taagatcact cttttctttt aaatattcat
       61 tagcatggtt cattatattt aacatcttct taaaagtatc actattttga taattaagtc
      121 gtttaactaa ttttgtaata taatcttgtt gataaacata attacaacta tactgataat
      181 tcaaatcaaa tatcaaggcc aaaaaggata cccagcgatc caagtcagtt ttacgagtgg
      241 tactaagaat tgattcacta ttcataaatg tttcatatat cgattcagta atgatgtcat
      301 tctctaaaat ctcttgtcca gcatccaaaa atgcttcgaa cgtttctaac aaacctgttt
      361 taaagatatc gattttgtca gcatcacgaa ttattttaca gtggagcatc tcatgttcac
      421 ttaatccctc agcaatttta tatttattat gattaaaaat agctttgtag atcagtggat
      481 aatctttttg atcaatatca aatttactaa ttaaattatc atcaaataaa atttgaacac
      541 ctaataatgc atgatcaatt gttttactat caataaaaca attataacgt tttaattgtt
      601 caaatctccc aatatcatgt aataatccaa ttaatgatgc taagtctatt tcatcttgat
      661 ctaacccgag actcactgca acattttttg aggcctccat aacacgataa gtatgttcta
      721 tctttaaggc aactttagga ttatctttat cataattcgc tacatattca tcaaacaatc
      781 ttttagcatt ttttaaatct atcatctaac cacctccagc tatcattata tgctctttaa
      841 cagcatttct ctactatttt ctaaatagta atttctattt ttatatggtt atgttatatt
      901 aataaacaag gatgtgatta gatgaaatta agattaacta ctactaatga tttaaccgct
      961 gtcatgacaa ttatcaatca agctaaagta tattttaaag agcagggaat caaccaatgg
     1021 caagatggtt atcctgatga attaactatt attaatgata ttagccgcca tgaagcttat
     1081 gtgcttgaag ataacggaga aatagtcgca actgcgatga tctctaaaga gcttgaacct
     1141 agttataatt atatagaagg gaaatggctt caagataatg catacattgt agttcatcgc
     1201 atcgcgatac gaaatgatca aaaaggtaaa ggcttggcaa aaatcattat tgatgaagga
     1261 ttaaaacttt atcctaaaaa accaagtatc cgtatggata ctcatgatga taatctttca
     1321 atgcagcgtt tcttgattaa atatggattt gaatattgtg gcactattta tttagaaaat
     1381 aaagaaactc gacgagcata tgaaaaaatt ctttaacctg cattacaaag tgcaggtttt
     1441 attgttcaat aaaatcaatc aatacttgat taaaacgttc tcgttcatca taaaaaatcc
     1501 catgtccact atattcaaac ttgcataaaa tcgaatcttc tatacattca tgcatgatta
     1561 aagcaaacga aaaaggacaa attttatcaa gcactccatg catgatcaaa gtagtgacct
     1621 taatctcaaa caaatcttga tacacatctt catctcgtaa agaaattgct gtttggatcg
     1681 ttcctattcc tgatcccttt aaacaaattg atgtaaacca gttcataaat tcactgccat
     1741 gatttaatgc aaaaacattt tgtccgaaat catgaaccat tttaggtcga ttagtataac
     1801 actgctcaat cagttgattt accgcatcga tactttgtcc ttcgggatta tgaattgtac
     1861 gagtatatga aggcactgca gcaccagcca aaactagttt acttactcgt tcttttccaa
     1921 atttactcat ataacgaaca caaatcgccc cacccattga aaatcctact aatgtcacat
     1981 tatggacatt taaggttatt aaaacactat gcagatctat cgctaattgg tcataatcat
     2041 agcccctgcc catgacttgt gacattccta atccacgaat atcataacta atgatgcggt
     2101 atcgacaatc gtttaaaata cctttttgat attcatacat ttcttggcaa attggccagc
     2161 catgcactaa aactactgtc ttttgatgag ctggattaat atcctctacc gctaatttgg
     2221 cattatctga tgattttaga taaaacacat aatcccccct catcatattc tatgtaagct
     2281 caataatatt ataaccttat tttaattatt aatttttgta taccagcagt cgattaagtt
     2341 aatcaatatt tactatgatt cgctatcatt aattttgata aattatattg tgatattgga
     2401 gtgagaatat taatatatat catcagtgca attattatct atgggtatag ttgttattat
     2461 tttaccagta ctatcgctaa tcttttaatt gcattaatag ccttatgagt atttggtata
     2521 tttatcatcg gattaatttt cttaaggaat gtccgcataa aaaataatta tagtggtctc
     2581 ttgtaagttg aaacattcct tattctcagc ttgtttttaa atgattttaa tagcttgagc
     2641 gactataacc ccattattct ttttactaac agaaatatta ctatgacata taatatttct
     2701 gttagtaact tgataattac aataattttg actattatct tagttatttt caatatttat
     2761 atctttaagt gtaaaaatta tttcatttgt attcatatct tattgtattc agcatatttt
     2821 tatatgaggt gaagaacatg gatactaaag taaataagcc ttaccccgaa ataaaagtaa
     2881 ctgtgccaaa tgaaacttat ggtttaatga tccttgataa tgtcggtggg atgaattctg
     2941 aaacatctgc aatttgtcaa tatatttata accatagtat tgctggcgaa gattttttag
     3001 aactaaaaaa aacattttta aatatttcta tggtcgagat gcatcattta gacatcttca
     3061 tgacccttgc cttagaatta ggtttagacc cccgctggtg gagctgtcta aatgatcaat
     3121 gtacttattg gtcaccatca tatttaaatt atcctaattc actagaggaa gtactaaaaa
     3181 cagcaataga cgctgaatat caggcaattg ataagtatat gtatcaagct agcgtcatca
     3241 aagatcccta tattgttgcg attttaaatc gaatcattga agatgaagaa ctgcatatca
     3301 aggttttaaa aaaatgggaa actaaattag ttagataatc taactaatta gttttccttc
     3361 ttttaataat tgcaataatt gtaaattttg cgccgcacta cctacataat tagtaattcc
     3421 attaagtttt gctaaatgtt gacgattttc aaaacttgta tcaacattaa tttgctgaag
     3481 ggctccaact aaactatctc caacataatt aggatttgat aaatagctta ccctaccttc
     3541 aattacctgg tcattgaccc agccttgatc attacctatt aaataaggat ttcgagtatt
     3601 ttttacaatt cgagttatct ttccaacatt acgatatggc cgtaacggat tagaagattc
     3661 actagttaaa aagacatagc taaatcttac tgtatcacct aannnnnnnn nntacctgca
     3721 cgtttaataa tagctggaaa atcagcataa caataattca tatcaacaat tgttggtacc
     3781 ccttgaaccc atccagtcga actatattgc cacatatcat acggtttatc ataatttaac
     3841 tcagcagcat agttagcaac ccaccgtgta taattatcaa aaatcggatc cgttaattta
     3901 gtgttccacc agtatagtga agcatacatc ccaacataat atccattggc ttctaattca
     3961 tcagcaaatg ttttagcgat atttgcaatc acatcatttg attgctttcc agtcgtatta
     4021 ttatcttcta aatcataata cactggatat gccatcttat agtttttcac tagacgtaaa
     4081 acatgttgtg cttcccctct agcatcacta ctatttttag catatgaata taagtaaaca
     4141 ccaaacggta ttcctaatcg tgtacactcg tctgcatttc ttttaaacaa acgatcatct
     4201 tgatctgttc gatcttgacc aaaaccacat ctaagaattg caaaatcaac tcgatcttga
     4261 acaatttccc aatcaattac cccctgatat tgtgagacat caattccatg tgtcgtcatt
     4321 cttccacctc cattaataat atatgagcaa ttgtcaaaaa tgtgtgactt tctagacttt
     4381 tttatcattt taatgcgaaa caatgaatat tatgttataa tcacattggt atggattaaa
     4441 tacgccatct ttaaaactga atataaaact aaataatata tataaattgg aggtcgatga
     4501 aatgaaaaaa gtattcgtca ctgccttagg agtctgtaca atgctttcat tagtaactat
     4561 tcccactcct gttcaggcaa ctgatttttc cgggcaagaa gataagtaca tgaaattatg
     4621 ctcatcttca aatctttcaa ctaataactt gaacacttgt aaagaattca atacatactt
     4681 gaaaaataaa aataaagaat tgaaatctca agtaagtgat tcaaaaagca aagtatctga
     4741 tactcaaaat agtttgaata gtatctcaag tgaaattagt gcattaaatg atcaaattgc
     4801 tgaaaaacaa aaagaaattg aatatttaca aacttcaatt tcaaatttag aagcaagtat
     4861 agctaaaaaa gaagaagaag taaaagaacg tatgtattca atgcaatcat acaataacaa
     4921 taattcatat attgacttta tcttcggggc ttctagcttc actgatatgt ttgcccgaat
     4981 tgacagcgtt aatgaaataa catcgtatga tgatgaatta gttgcacaac tagctgatga
     5041 aaaagaacaa gtagaaacac aaaaagctac ggttgttact gccaaagcta atattgaaag
     5101 tcaaaaatca tcaaagcagg cactacaaca agaatatcaa gctctatttg aaaaacaaaa
     5161 cgctgatttg attgcgcaag aaaaagcagc tgctcaagcg gccgattcaa gtcaaaaaat
     5221 cactgataat atcgctgcac tattagctgc tactgaaaaa tcacaagttt ctggcggaag
     5281 cgttgtttct ggtgattctg cagttggaaa cgcaattgca caaaaggctt taactcgcgt
     5341 tggttatatg tatgtatggg gtggatgtca cagcatgtct gaaatcgcta atccaaacca
     5401 cactgcattt gactgctcag gtttagtcaa ctgggcttat tatcaatcag gtgtcaacat
     5461 tggttctaat aataccaaat cgctagctag taaaggtgta tctgtttctc gtaataacat
     5521 gcaggccgga gatatcatct tatttagcag taatggaagt accagcggaa ttcaccatgt
     5581 cggtatttat atcggtggtg gtaacatggt tcatgctcca caaacaggta aacctgttca
     5641 agtagcggat cttggttatt catattggca aaaagaatgg tatgacgttc gtagattgta
     5701 ttaatacaat tagatgagca agcttgctca tcttttttat cacatatata tgaaattcat
     5761 aaattaattt cattatttcc ataacaaatg tgatattttt tagaaaatta gctttatatc
     5821 cttagaaaaa atctaaatta gtatttactt attaagaaaa atataggata attactatgt
     5881 atcgaggtga ttgtatgaaa ataaaaaaag ttatatctac agctttaacg ctgacaatag
     5941 taacaagtgc atgttattat gcagcccctg tggcagccga tgaatttgat ggtaatgaaa
     6001 gtaaatatat gacattatgc tcatcttcaa atttatctag tagtaacaaa tcaacttgtg
     6061 aagcattcaa caagtatctt aaaactaaaa attctgaatt aactaaaaaa ttggccgaac
     6121 aaaaagaggc tgctagtgac actaaaacaa cactagaaag cgttcaaaaa caattagatg
     6181 aagttaataa acaaatcagc gataaagaag ctgaaattga gtatttaaat acaactattg
     6241 ccaatcttca agcaagtatt gaaaagaata ctcaattatt aaaagatcgt atgtatgcaa
     6301 tgcaatctta tgcaaatgaa aatacatata ttaactttat ttttggtgca agcaacttca
     6361 cggaaatgtt ctcaagaatc gacggttata acgaacttac acaaagtgat aaagagttga
     6421 ttgaagaact taataaacaa agaaaagaag ttgaacaaca acaaactatt ttagaagaag
     6481 ctaaagccac tttagttatt cagcaagaag aacaaaaaac attaaaaaca aaatatacag
     6541 ctttgttaga ggaacaaaac aaatcaattg catctactca aaacacgatt tatgattatg
     6601 gtgaaatgac tgaaaccctg gatgctgcaa ttaaaaaatt taatgaagaa gcatatgaaa
     6661 ctccaactcc tacaccaccg cctccaaaac ctgataatgg tggaaacaat aataattccg
     6721 gtggtaacaa caacaacaat aataacaata gcgattctgg aaataacaac aatggcggaa
     6781 acaataataa tgattctagc acatcagata atcttggtga aaaaatctat gctgctgctt
     6841 atgctaaatt aggatcgcgt tattggtggg gagctcaagg tcccacatac tttgactgtt
     6901 ctggtttagt atattggtca ttaaaacaag caggtgttag tggtggtcga gatactgctg
     6961 ctggttactc cagaaaatgg tcagctgtaa gttttgcaaa ctctaaaact ggtgatttag
     7021 tatgttttgg tagtccagct tatcatattg gaatcattgt tgtaaacagc gatggttcaa
     7081 gatcaatggt tcatgccggt ggtggtgatt catcaacaca cggtgataat ccaaaagctt
     7141 atgttaaagt atcttcaatt gaaccaggat catattacta tagtagaata agtacaatta
     7201 ggcgtgttag taaataatag atattttaaa ggacaaattt tgtagttcac tacaatactt
     7261 gtcctttttt attttaggcg ataattatcc aaataatata tgatcgttat gttatattta
     7321 tatcctattt aaaatttact atgctataat taaagttaga aaacaaagga gggaaatgag
     7381 catgaaagca aagataatat ccctctttca ttacttatta gtctttttat taataatttt
     7441 taataccagc tgtcaagcta aatcaataaa tattgttgat acaaataata atgatcagat
     7501 tgaactaaat ttttatggat ataaaacgga agcaattaat gttgttgcaa tcgaagaaat
     7561 tttacaagct tatatggata aaaatcccaa tattaccatt acctatgaaa gtgtgaaagg
     7621 aactgagtat tatgagatac taaaaaaacg tttggcctct aataactatg atgacatttt
     7681 tatgatcgat gaagataatt tacagcaatt aactaattat aattattttg aagatttatc
     7741 aaagctaaaa acaataaaaa actttaacac aaactcattg gaacaaatgg tgcaaccaga
     7801 taatacgatc ccctatattc caacttctat ttcagcattt ggattatatt gcaatcttga
     7861 tttactagaa aaacatcatc agtcagttcc taaaaataat caagaattta tggctgtttg
     7921 tcaatatttt attgagcaag gaattactcc gatcatcgct aataatgata tttctctaaa
     7981 aacaattgct cttgctaaag gtctttatcc catctatcaa tcacctgaaa aggatagaat
     8041 aatcgcttca ctaaatcaag atcctagcat tctttgtaat tatcttaaag aaggatatca
     8101 atatgttaaa cagttaatcg acaataaatt cattgatgca gccacaactt taaaaacaga
     8161 aaaaactgca gacgatctaa tacagtttga agaaaataaa aaccctttta tgttaactgg
     8221 tgcctgggcc tctgttcgag ttgaaaaaag ttcaccagat ttaaatttta aagtttaccc
     8281 ttatccaatt ttagacgatg gagcaattct agtttcaaat attgataccc gtgtatgtat
     8341 taatgctaaa ggtccacata ttactgaagc aaaaaaattt attgaatatt taagtcaaga
     8401 agatgtgatg tggaaatttg taaatagtca gtcctctttt agtcctctga aagaacaacg
     8461 tattgctgat gatcctaaga ttcaagaatt aagcccttat ttaacctcag agaaaacaat
     8521 tctaggcagt gattcaaata taaatcttcc tctttggtca cttacccatg acggaatcac
     8581 ccaattatta acaggaaaca gtattgatag tgctttagaa ataatcaaaa attatgaaca
     8641 ggaggatgat tccaatgaaa aataaaaaaa tatcattaat gattgtcggg ggaattagtg
     8701 ctgtgatttt tgttgttttt tccatgttca tttatgttaa ttatgttcaa gattctcttt
     8761 gggataagtc aatcaatgat attttagaaa caacctctca ggaggaaaaa gcctttaatg
     8821 tctatatgac taaagaaatg actaactccc aaaacatcct tactaatatt gcctatctac
     8881 aaactaagat tccgcagtat ctccagcatc tatctaatcg tgaagaagct aaatatttat
     8941 atatcgacta taccaatgac tgctactatg atgaaaatgg taattatccg ttaggtgatg
     9001 aacagttgtt tcagcaaata aaagctgata ataattcaca aggattttta gagccctatc
     9061 ttaattcaaa aacaggtatt aagacttttg caacttatgt aaaaacaagc gatagtcaaa
     9121 gtgtcattgt caaagaaact caattaagtt ttattattga aaacttctca ctttcgtttt
     9181 ataacgattt aggtttttca tatattgtta aagataatgg tgaaatttta attcgttcca
     9241 aacatcgt
//
LOCUS       DAOMTA010000015         9087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_63_length_9087_cov_2.447409, whole genome shotgun sequence.
ACCESSION   DAOMTA010000015 DAOMTA010000000
VERSION     DAOMTA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_9087_cov_2.447409"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..296
                     /locus_tag="PLK68_00935"
     CDS             123..296
                     /locus_tag="PLK68_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89004.1"
                     /translation="MNKLALVAISLFVVVEILVGDCVVQGIPLWVVGLIGFSLIWMGI
                     ICVKEKVQIKDWY"
     gene            complement(324..1490)
                     /locus_tag="PLK68_00940"
     CDS             complement(324..1490)
                     /locus_tag="PLK68_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRM89005.1"
                     /translation="MKFINSLKERFTKEQLMIIATALSLSTPFYICVPFLLLETLYLL
                     YTKKIINAFKSTPKSKYLIIFMLLTLAISLIYQNWIGVGCVALIFIFVSLMLYYRQYI
                     NEDTFEFILDMLIALSILWAIYGLFEQIQILDRLGYDHFTLKVFSRRENRLNSVFFNA
                     NYYAMMIEFTIMMIGYKIFGTKNVKKQLYYFIVACLNFFLLYMTGCRTAFVATAGAML
                     VFLIINKNYRICSLIGLLCIIGGIYFIFNPEQFPRIEKLVDNFTVRTKIWHAAIEGIK
                     AHPLFGQGPMTYMMIFKQYGGHVTQHAHSVYLDPILSFGVVGVATLVPYMFDNCKRLF
                     KVYQEKLNLRYVALVISCIAVILLHGVLDYTIFWVHTGMLFLLIASSFEMFKHK"
     gene            1555..2490
                     /locus_tag="PLK68_00945"
     CDS             1555..2490
                     /locus_tag="PLK68_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRM89006.1"
                     /translation="MKKYLYVFNYPPEDKELCALEFRTLFKDEFKSKYYLSNKDYSVD
                     KSVFVKAKLDLWGIDADFNKLIEKVAALHKDYQNFKVIYLKNEVTHVDYQESLLRCKD
                     ISWGIAGSVNMSRPQHTIAITKLEDQWLCGYYHHGVPSWQKHDDKPNTFSNSLDIRLA
                     RTLVNLAAGDNDQVTIVDPCCGMGTVVLEGLALDLDIEGFDISREISWQARKNLKYYG
                     YDEYLINKVSIHDLQKHYDVAIMDIPYNLYTPITYEEQCRMIQSSRRICDKMIMVTFE
                     EMSTEINQAGFLIIDGCLRKKTEYVKFGRYIYICI"
     gene            complement(2513..4096)
                     /locus_tag="PLK68_00950"
     CDS             complement(2513..4096)
                     /locus_tag="PLK68_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRM89007.1"
                     /translation="MKKNSIFKQAAFLAAAGILVRIIGLLYRSPLTKLIGSQGMGYYS
                     TAYNVYALILLVSSYSIPTAISKLLSEKLAVNQYNNVKKILLCSFIYIVAVGGGAAII
                     AFVIAPYIVPDKAVSALRILCPTIFLSGLLGIFRGYFQAFKTTAFTEISQIIEQVFNA
                     GVSIGAAYLFIQPYLNNQSLVASHGATGSALGTGAGVLISLCYMLFMFKRTKQSYLNP
                     PNIEAADPHTDSFKDIFKMISNIVTPIILATCVYNLISTIDMYMFYLVCGDGAKSISA
                     FGAYGGEYIILQNVPVALASAMSTASIPNISSAWLFKDTAEVKKQIAQGTKVIMLILI
                     PSAVGMSILAVPIIQAIFPQKETVVLASTLLTFGSPAIVFYGLSTYTNGLLQALGYSS
                     IPLKNAIYALIIHCFITLFLLLATNLNIYSLLIGNCLYGLQLCFANQRALKHITTYYQ
                     EKLHTFIYPLISSAIMAFTVALCYYGLIKVIDRLIITLFIAIVIGVIIYFGTLLFLYK
                     DNVEELSQIPYLQKIIKHK"
     gene            4341..5168
                     /locus_tag="PLK68_00955"
     CDS             4341..5168
                     /locus_tag="PLK68_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89008.1"
                     /translation="MFMEGDKMFSIGEFSKMAKTTVKTLRHYDEIGLLAPAYVDTYSK
                     YRFYTSNQLVIIHQIQSLRQIGLSLDEIQMIINGVDGVAILESRKKAIKESISEAEDQ
                     LSRIEFILSGKEEDTFMNYQASIKELPACTVYSKRMVVPGYDSYFELIPAIGQKVMNK
                     YPDLKCTVPEYCFIIYRDGEYKEKDIDIEFCEAVDQVKVDFDDIKFKKMPAVKAVSIM
                     HKGDYAGLPKAYAYAFKWIEENGYQVLDNPRESYIDGIWNKESKDEWLTELQIPVTK"
     gene            5254..6372
                     /locus_tag="PLK68_00960"
     CDS             5254..6372
                     /locus_tag="PLK68_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM89009.1"
                     /translation="MKLLKYVKEYRFPAIIGFVFKIAEAALELMVPLVMADIIDVGIK
                     NNDQNYILVRGLFLVGLAVAGYLFALVCQYYASLTSQSVGTKLREDMYHQINRYDHHN
                     LDKLSAPTLVTRLINDVVQIQLAVAMTIRLTSRAPFIMIGSLFLAFLISGPLASIFVV
                     GAIVLAIVMLMITIISMPYFNNVQKKLDKISLIVRENLNGIRVIRAFASQDKEINKFK
                     TETRQQKDIQVKVGRIQALLNPFTYLIVNVAIVLIVYFGGTEVNVGGLSQGEVIALVN
                     YMNSILLALIVFANVLSIYNKAGASYTRIFEVLETEPAVVNDGQIQIYRESEDCIEFR
                     HVSFAYEQKNVLNDLNFTIKRGQTIGIIGGTGAGKTSL"
     gene            6417..6971
                     /locus_tag="PLK68_00965"
     CDS             6417..6971
                     /locus_tag="PLK68_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89010.1"
                     /translation="MNGEAIKNYDLHALRSFIGFVPQYAALISGTIRENLQLGNQTAN
                     DQQLLKALEIAQGKEMLEDKAAGLDTVIEQGGKNLSGGQKQRLTIARALVKQPEILIL
                     DDSSSALDYATDFKLRQALKQLNMTKIIISQRTASIEQADKIMVLYHGDLVGFDSHEQ
                     LMKDCKIYQEIYASQHSKDGDDHE"
     gene            6964..8700
                     /locus_tag="PLK68_00970"
     CDS             6964..8700
                     /locus_tag="PLK68_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89011.1"
                     /translation="MNSKTIKRLLTYCRPYRVILGLIFILSFISVCLTLITPILFGQA
                     IDLLIGIGLVDFSRLFWQLLVIVGVVLAGALVQWILGQLTNKITYNITNDLRDRVFEK
                     IHLLPLKYIDSTPHGDIIGRVINDIDLIAAGLLQSFTSLFTGVATIVGTIIIMCLINL
                     SIAIVVIVLTPLSLLVASLIVKRTHIYFKEQLELRGEMNGYIEEMIGNQRIIKAFNYE
                     QMNEERFKEVNQRMHVSGVKSQFYGALINPTTRIVNSLVYGAVGVFGAISVLNGSFTV
                     GLLSSFLTYANQYTKPFNEISSVMTEMQTALAASQRVFNLLDEPVEKPVSNPQNVEIM
                     EGNVSLKNVYFSYDPKVSLIENLSLEALPGQTIAIVGKTGCGKTTLINLLMRFYDQNS
                     GSIMVDNVSTLDMERDYLRRMYGMVLQESWIFKGTIKENIAYGKSDATDEQIIAAAKK
                     ARVHKFIMKQPEGYDTMIDEDGGNISQGQKQLICIARIMLTKPPMLILDEATSSIDTR
                     TELQIQEAFETMMKGRTTFIVAHRLLTIKNADMILVMDKGHILEQGTHQELLEKQGYY
                     YNLHNSQFNLAK"
BASE COUNT         3296 a         1153 c         1689 g         2949 t
ORIGIN      
        1 ttttcatatt tcataaattc caaaatttaa cattttgtta acaaattgat gatattatta
       61 gtctacaaaa taagtattat aaagataagg ataaacatat atttagtaag gaggcggttt
      121 taatgaacaa attagcttta gttgcgataa gtttatttgt agttgtagaa attctagttg
      181 gagattgcgt agtccaggga attcctcttt gggtagttgg tttaattggt tttagcctaa
      241 tttggatggg aataatttgt gtaaaagaaa aagtccaaat caaagactgg tattaaaaaa
      301 aggaaattat gatttccttt tttttattta tgtttaaaca tttcaaatga tgaagcaatt
      361 aataaaaata gcatacctgt gtgtacccag aaaattgtat aatcaagaac accatgtaat
      421 agaataactg caatgcatga aattacaagg gcgacataac ggagatttag cttttcttga
      481 tagaccttaa ataatctttt acaattatca aacatataag gaacaagagt agcaacgccg
      541 acgacaccaa atgataaaat cggatcaaga tagacgctgt gagcatgttg agtgacatga
      601 ccaccatact gtttaaaaat catcatatat gtcattgggc cttgaccaaa gagcggatgg
      661 gctttgattc cttcaattgc agcatgccag atctttgttc ttacagtaaa gttatcaact
      721 agtttttcaa ttcttgggaa ctgttcagga ttaaagataa aataaatacc accaatgata
      781 caaagtagcc caattaaact gcaaatccgg taatttttat tgatgatcaa gaatactaac
      841 attgctccag cggttgcaac aaaagcggta cgacatccag tcatatataa taaaaagaaa
      901 ttaaggcagg caacgataaa ataataaagc tgttttttga catttttagt accaaatatt
      961 ttatatccaa tcatcatgat ggtaaattca atcatcatgg catagtaatt agcattgaag
     1021 aaaacagaat taagacgatt ttcacgtctg ctaaatactt ttaaagtaaa atgatcataa
     1081 cctaaacgat cgagaatttg aatttgttca aatagtccat aaatggccca tagaatcgat
     1141 aatgcaatta acatatctaa aataaattca aaagtatctt cattgatata ttgtcgataa
     1201 tataacatta gtgaaacaaa gataaagata agtgcgacac atcctacacc aatccaattt
     1261 tgataaatta aagaaatagc taatgttaaa agcataaaaa ttattaaata tttagatttt
     1321 ggggtacttt taaacgcatt tataattttt ttagtgtaca ataggtataa tgtttcaagt
     1381 aataaaaaag gtacacaaat ataaaatggg gtagataaac taagggcggt agcaataatc
     1441 attaattgtt ccttagtaaa acgttctttt aatgaattga taaatttcat aatagacctc
     1501 caagtactgt tactatagca taaatataaa atagacacaa acaaaggaga tataatgaaa
     1561 aagtatttat atgtttttaa ctatccccca gaagataaag aattatgtgc tttagaattt
     1621 agaacattat ttaaagatga atttaagagt aaatattatt tatcaaataa agattatagt
     1681 gttgataaaa gtgtttttgt aaaagcaaag ctcgatcttt ggggaataga tgctgatttt
     1741 aataaactaa tcgaaaaagt ggctgcgctg cataaagatt accagaattt taaagtaata
     1801 tatttaaaaa atgaagttac tcatgttgac tatcaagaaa gtttacttag gtgtaaagat
     1861 atttcttggg gtattgcagg aagtgttaat atgtctagac cgcaacatac tatcgcgatt
     1921 acaaaattag aagatcaatg gctttgcggt tattatcatc atggtgttcc gtcttggcaa
     1981 aaacacgatg ataaacctaa tactttttct aattctttag atattcggtt agctcgaact
     2041 ttagttaatt tagcagctgg agataatgat caggtaacga tcgttgatcc ttgttgtggt
     2101 atggggacag ttgttctaga aggactcgct ttagatttag atattgaagg attcgatatt
     2161 tccagggaaa tatcttggca agcacgtaaa aatttaaagt attatggtta tgatgagtat
     2221 ttgattaata aggtgagtat tcatgactta caaaaacatt acgatgttgc aatcatggat
     2281 atcccatata atttatatac tccaataact tacgaagaac agtgtagaat gattcagtcc
     2341 tcgcgaagaa tttgtgacaa aatgattatg gtaacttttg aggaaatgag tacagaaata
     2401 aatcaagctg gctttttgat cattgacggc tgcttacgta aaaagacaga atatgttaaa
     2461 tttggacgct atatttatat ttgtatttaa taaaagcaac atttagttgc ttttatttat
     2521 gttttatgat tttttgaaga taaggtattt gtgataattc ttcaacatta tctttatata
     2581 gaaaaagcaa tgttccaaaa tatataatga ctccaataac gattgcaata aatagagtga
     2641 taatcaaacg atcaataact ttaattaaac catagtagca tagcgcgact gtaaatgcca
     2701 taattgctga actaatcaat gggtagatga aggtgtgtaa tttctcttga taataagtag
     2761 taatatgttt taaagctctt tgatttgcga aacaaagctg taaaccataa agacagttac
     2821 cgatcaatag agaataaata tttaaattcg ttgcaagtaa aagaaataga gtaataaaac
     2881 aatggataat taacgcatat atagcatttt ttaaaggaat agatgaatat cctaaagctt
     2941 gcaataatcc attggtatag gtagaaagac catagaaaac aattgctgga ctcccaaatg
     3001 ttaaaagggt gctggctaga acaaccgttt ctttttgcgg aaaaattgct tgaataatcg
     3061 gaactgcaag aatactcatt ccaacggcac tgggaataag gatcaacatg atgacttttg
     3121 ttccttgagc aatttgtttt ttaacttcag cagtatcttt aaatagccat gctgatgaaa
     3181 tattgggaat tgaagctgtt gacattgctg aggcaagagc tacaggaacg ttttgtaaaa
     3241 taatatactc acccccataa gccccaaagg cactgatgga tttggcacca tcaccacaaa
     3301 ctagataaaa catatacata tcaatagttg agataaggtt gtaaacgcaa gtagccaaaa
     3361 ttattggagt aacaatattt gaaatcattt taaagatatc tttaaacgaa tctgtatggg
     3421 gatcggctgc ttcgatattt ggaggattta aataactttg tttagttctt ttgaacatga
     3481 acaacatata acataagctg attagaactc cggcgccagt gcctaaggca ctaccagttg
     3541 ccccgtgact agcaactaga ctttgattat taaggtatgg ttgaataaat agataagcag
     3601 ctccaattga aacaccggca ttaaatactt gttcgattat ttgtgaaatt tcagtaaagg
     3661 cagtagtttt aaaggcttga aaataacccc taaaaatccc caatagtcca gaaagaaaga
     3721 tcgttggaca aagaattcgc aaagctgaaa cagctttatc aggaacaata tagggtgcaa
     3781 ttacaaatgc gataattgct gctcctccac caactgcaac aatataaata aatgagcaaa
     3841 gaagaatctt tttaacatta ttatattgat taacggccag tttttcagat aacaacttag
     3901 aaatagcagt tggaatactg taagaagaaa ctaaaagaat tagtgcatat acattataag
     3961 cagtagaata gtaacccatt ccttgtgaac caatcagttt agttaatgga cttcgatata
     4021 acagaccaat aattcttact aatattccag cagcagctaa aaaagcagcc tgtttaaaaa
     4081 ttgaattctt tttcataaaa taaactcctt atggataata atgtcatatt atattaacca
     4141 taccatttat tgaaggataa atgtatgaaa attttaatta ttaataagat tcttgtttta
     4201 attattttat tttatcataa aaagataatt agttctttat tgtcggtagt ttaaataaaa
     4261 aagataaaat atgttagaat agggtgatta gaaaattaat tttatatttg cttgactctc
     4321 cggtaagggg agcttatacg atgtttatgg aaggtgataa gatgttttcg attggtgagt
     4381 tttcaaaaat ggctaaaacg actgtaaaga ctttgcgaca ttatgatgag attggattgc
     4441 tggcgccggc atatgtagat acttatagta aatatcgttt ttatacgagt aatcaattag
     4501 taataattca tcaaattcag tcattaagac aaataggttt atcattagat gaaatacaga
     4561 tgattattaa tggtgttgat ggtgttgcaa ttctagaaag tcggaaaaaa gcaataaaag
     4621 aaagcattag tgaagcagaa gatcaacttt cacgcattga gtttatttta tctggcaaag
     4681 aggaggatac atttatgaat tatcaagcaa gtattaaaga attaccagca tgtaccgtat
     4741 attcgaaaag aatggtagtc cctgggtatg acagttattt tgaattgatt cctgcaatag
     4801 gtcaaaaagt gatgaataag tacccggact taaaatgtac agttccagaa tattgtttta
     4861 ttatttatcg agatggtgaa tataaagaaa aagatattga tattgaattt tgtgaggcgg
     4921 ttgatcaagt gaaggtagat tttgatgata tcaagtttaa aaagatgccg gcggttaaag
     4981 cagtatctat aatgcataaa ggtgattatg cgggattacc taaagcatat gcgtatgctt
     5041 ttaaatggat tgaagaaaat ggatatcagg tacttgataa tccccgtgag agctatattg
     5101 atggaatctg gaataaagaa agtaaggatg aatggctaac agaattacaa ataccggtga
     5161 ctaaatagcg gattgggaat tagattaaat tctaattcct tttatttttg tggaaagata
     5221 gggtataata aatccagatt tgggggttag acaatgaaat tattaaaata tgttaaagag
     5281 tatcgatttc ccgcgataat tggatttgtt ttcaaaattg ccgaagcagc cttagaatta
     5341 atggttccat tggtaatggc tgatattatt gatgtaggaa ttaaaaacaa tgatcaaaat
     5401 tatattttag taagggggtt atttttggta ggattagcgg tggctggtta tttatttgcg
     5461 ttagtatgtc aatattatgc atcacttaca tcacaaagtg ttggaacaaa attgcgtgaa
     5521 gatatgtatc atcaaattaa tcgttatgat catcataatt tggataaatt aagtgcgcct
     5581 accttagtaa ctagattgat taatgatgtt gtacaaattc aattggcagt agcaatgacg
     5641 atccggttaa cttcacgggc accgtttatt atgattggtt ccttgttttt agcgtttttg
     5701 attagcgggc cgctggcttc tatttttgta gttggggcta tcgttttagc aattgttatg
     5761 ttgatgatta caataatttc aatgccgtat tttaataatg ttcaaaaaaa gcttgataaa
     5821 attagtttga tcgttcgtga aaacttaaat ggaattagag taattcgagc atttgcttca
     5881 caagataaag aaattaataa gtttaaaaca gaaactcggc aacaaaaaga tatccaagta
     5941 aaagtaggga gaattcaagc attattaaat ccatttacat atctgattgt taatgtggct
     6001 attgttttaa tagtttattt tggtggaact gaagtaaatg ttgggggatt atcgcaaggg
     6061 gaagttattg ctctagttaa ttatatgaat tctattttat tagccttgat tgtttttgct
     6121 aatgtgttat cgatctacaa taaagcaggt gctagttata caaggatttt tgaggttttg
     6181 gaaacagaac cggcagtagt taatgatgga caaatacaaa tatatcgaga aagtgaagat
     6241 tgtattgaat ttagacatgt tagctttgct tatgaacaaa aaaatgtttt aaatgattta
     6301 aattttacga taaaacgtgg tcaaacaatt ggaattattg gtggaacagg agctggaaaa
     6361 acttctttgt aaatttaatt gggcgttttt atgatgttag cagtggcgag attttaatta
     6421 atggagaagc gattaaaaat tatgatcttc atgctttacg ttcttttatt ggatttgtac
     6481 cacaatatgc agctcttatt tcaggaacga ttagagaaaa tttacagtta ggaaatcaaa
     6541 cagccaatga tcaacaattg ctcaaggcct tggaaattgc tcagggaaag gaaatgctcg
     6601 aagataaagc ggctggatta gatacagtaa ttgaacaagg cggaaagaat ttatctgggg
     6661 gtcaaaaaca acgtttaacg attgccagag cattagttaa acagccggaa atcttaattc
     6721 ttgatgatag ttcaagtgca cttgattatg caactgattt taaattgaga caagcattga
     6781 aacaacttaa tatgacaaaa attattatct cacaacgaac agcttcaatt gaacaggcag
     6841 ataagataat ggttttatat catggtgact tggttggctt tgatagtcat gaacagttaa
     6901 tgaaagattg taagatctat caagaaatct atgcttcaca acatagtaag gatggtgacg
     6961 atcatgaata gtaagacgat aaagcggtta ttgacatatt gtcgaccata tcgtgtgatt
     7021 ttaggtttaa tatttatctt atcttttatt agtgtctgtt tgacattaat tacgccaatc
     7081 ttatttggac aagcaatcga tctattaata ggaataggac tggttgattt tagtcgtttg
     7141 ttttggcagc ttctagtgat tgtaggagtt gtgcttgcgg gtgcattagt ccaatggatc
     7201 ttggggcaat taactaataa gattacttat aatataacga acgatcttcg tgatcgcgtc
     7261 tttgaaaaga ttcatttatt accattaaaa tatattgata gtacgccaca tggtgatatt
     7321 attggtcgag taattaatga tattgatttg attgcagcgg gtttattgca aagttttact
     7381 agtttattta ctggtgttgc aacgattgtt ggaacaatta ttataatgtg tttgattaat
     7441 ttatcaattg caatcgtagt gattgtttta accccgttat cattactagt cgcttcacta
     7501 atcgttaaaa ggacccatat ctattttaaa gaacagcttg aacttcgagg tgaaatgaat
     7561 ggctatattg aagaaatgat agggaatcaa cgaatcatta aagcctttaa ttatgaacaa
     7621 atgaatgaag aacgttttaa agaagtaaac caacgaatgc atgtaagtgg tgtaaagtca
     7681 caattttatg gagctttgat caatccgacg acaaggattg ttaatagtct ggtgtatggt
     7741 gcggttggag tatttggtgc aattagcgtg ttaaatggta gctttacggt tggtttatta
     7801 tctagctttt taacttatgc aaaccaatat accaaacctt ttaatgaaat ttcgtcagta
     7861 atgacagaaa tgcaaacagc tttagcagct agtcagagag tatttaattt attagacgaa
     7921 cctgttgaaa aaccagtcag taatcctcaa aacgttgaaa taatggaagg taatgtttct
     7981 ttaaaaaatg tatactttag ctatgaccct aaagtatcat taatagaaaa tcttagttta
     8041 gaggcattac caggtcaaac aattgcaatt gttggtaaaa ctggttgtgg aaaaacaact
     8101 ctaattaatt tattaatgcg cttttatgat caaaatagtg gaagtattat ggttgataat
     8161 gttagtacat tagatatgga gcgagattat ttacgtcgta tgtacggaat ggtattgcaa
     8221 gagtcatgga tatttaaagg aactattaaa gaaaatatag cttatggaaa aagcgatgct
     8281 acagatgaac agattattgc tgctgctaaa aaagcccggg ttcataaatt tattatgaaa
     8341 caacctgaag gttatgatac tatgattgat gaagatggag gaaatatttc tcaagggcaa
     8401 aagcaattga tttgtattgc ccgaattatg ttaacaaaac ctccaatgct gattcttgat
     8461 gaagcaacaa gctcaattga tacacgtaca gaattacaaa tccaggaagc tttcgaaacg
     8521 atgatgaaag gtcgaacgac atttattgtg gctcatcgtt tattgacgat caaaaatgcc
     8581 gatatgatct tagtaatgga taaaggacat attcttgaac agggaacgca tcaagaattg
     8641 ttagagaaac aaggttatta ttataatctt cataatagcc aatttaattt agctaagtga
     8701 tagatattta ataaaaaaga tacaataagc taaaatgatt ataaactata aaggtaaaga
     8761 taactgctgg aggtaaactt tagcagtttt tattatttta aaggaaatta ttcaaaaagt
     8821 attttgtgta taaaagaaga aatagggaca taaatatttt atacactaaa ataaaaatat
     8881 atgattatta tagtttagtg tataattgaa cttgtaaaaa ttaaaagaaa gcgtatacac
     8941 tacttataaa atatgctcta gtgtattaaa aaatgatgat ttatcaagtt tgcgagaaat
     9001 aattgattat tttatagttg taattggcta gagcaagtag tgtcaaagga gttagctatg
     9061 aaatcaaaca aagtaaaagt atttgag
//
LOCUS       DAOMTA010000016         9082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_64_length_9082_cov_2.715631, whole genome shotgun sequence.
ACCESSION   DAOMTA010000016 DAOMTA010000000
VERSION     DAOMTA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_9082_cov_2.715631"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PLK68_00975"
     CDS             complement(<1..822)
                     /locus_tag="PLK68_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89012.1"
                     /translation="MLRLNKFTKITSIYIITMMMITTIFTYFGYYAHKATIKDDWPTK
                     YVADDINDDLKYNYSRFTDVVIANIDRNVTSVYLAFDEDFQPFLAPHERWHFAAKNGD
                     NIAEVSLSEISYQKLKSICEKLIALMDEDKTKTINVTFNYSAITDNHIIVSNLVYFAI
                     NDEVYISNDSTATITCELTNLYTPFLDIQIPSDEDWNGYYYNSNIKIYNYLENFFKQN
                     YTKTQDDGYYKLSTPDDDPFKINNEAEENAYNFSYCIQKLSREYSHHDSFSAKEFD"
     gene            complement(944..2167)
                     /locus_tag="PLK68_00980"
     CDS             complement(944..2167)
                     /locus_tag="PLK68_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM89013.1"
                     /translation="MNKLTKLEKYWVLYDVGNSAFVLLVSTIIPIYFKNLATEAGISL
                     SDSTAYYSYAISISTLIVALAGPMLGSIGDRKGVRKPIFTFFMMMGVLSLAALSIPVG
                     WIVFLAIFIVAKTGFATSLIFYDSMLVDITTYERVDQVSSHGYAWGYIGSCIPFTISL
                     LLILGCDTIGLSTVMATAIAFIMNALWWFFITIPLLKNYQQINYTTAKEPIINNLKRV
                     LTSIKNNKKVLFFLIAFFFYIDGVYTIIEMATSYGKDVGIDDNSLLLALLLTQVVAFP
                     FSLIFGKLAKKFPVKSLILSCIIGYFFIAVFALWLDTAWKFWVLAVFVAVFQGAIQAL
                     SRSYYAQIIPESQASEYFGIFDIFGKGASFMGTLLMGITTQITDNSKYGVVVIACMFV
                     IGGIIFKSKCHNQAD"
     gene            complement(2160..2942)
                     /locus_tag="PLK68_00985"
     CDS             complement(2160..2942)
                     /locus_tag="PLK68_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRM89014.1"
                     /translation="MPASYTHQQFGNQVLNKLENTTIKKIINDNLNLYNIGLQGPDIL
                     FFYQPLKKNPVNTLGNQLHQDIARAFFENAMKLLKEQPNEKSLAYILGFINHFILDSE
                     CHGYIDKTMINREIGHFEIERDLDQRFMILNHESFNYSTPKHYQINLENAKIIAPFFN
                     LAPATILASLKSFKRFNHLFACRSVLKRSLILTGMKIVNAKSYCGMVMTAKPEKRIEE
                     NIDILVNLFNQSINIATKEIIEYYYSYQNNTAISKRFNYNYE"
     gene            complement(3145..3930)
                     /locus_tag="PLK68_00990"
     CDS             complement(3145..3930)
                     /locus_tag="PLK68_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRM89015.1"
                     /translation="MPAAYSHYRLGQLVLDKLNQPLKRVLETNQDLFDIGLHGPDILF
                     YNRPYIHSKINRLGSAMHKERADIFFYQALEILKETKSAPQFAYLCGFICHYILDSNC
                     HPYINTIIKETGVTHFEIETELDRYFMVKDRLDPLRTKLTDHIKVNDHTLNNIEPYFK
                     ATKKELYKSLKGMKFYDRLLLAPQFYKRGLIYLVLKITFTYKRFQGFVVNYRPNKLVD
                     PYLEKLESLFNQSISESYEAIYNFIDVIKYDRSLSYRFEHNFK"
     gene            complement(3930..4280)
                     /locus_tag="PLK68_00995"
     CDS             complement(3930..4280)
                     /locus_tag="PLK68_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HRM89016.1"
                     /translation="MINFIEYPKCSTCKKAKKYLDELGIDHHDRHIVEERLSKEELTA
                     LYQKSGLPLKRFFNTSGLKYKELQLKDKLPTMTEDEQLELLATDGMLVKRPIVETKDS
                     VLVGFKANEYDNLK"
     gene            4448..6355
                     /locus_tag="PLK68_01000"
     CDS             4448..6355
                     /locus_tag="PLK68_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HRM89017.1"
                     /translation="MLNKRQTNIIDLLNDYGKWITGKEIAKVLNVSDRTIRSDIENIN
                     NYYDCILIQANKRLGYRLDETLLHKQDIETKDIIPQTSQERCIYIIQELLFKSHEINL
                     IALQDQVFVSGYSIDNDIKKIRKMINDYPSLKLVRSKNYISLEGNETDKRKLYKQLLT
                     AETQGNFMNLNSIAGLWNSFDLLEVKDILEEICEKYDYQIHEMTFPMIMIHAGVAIER
                     IINHNYIKNQTISEKLESSREYQISYDFFTQVSTMINIELVTDEVILFALLLMGKRAN
                     DYRKDIIKEKLAVDVEHLVEAVIVEVKEYFDIDFSKDKDLKVGLAMHLQSLLERQKNN
                     VQVTNVYLQEIKRKYPLVFEMAVRAGEVLAETCEVNINENELAFLALHLGAAYDRVNS
                     LKRYRAIMIIPNNQMLSKMCRDKLKIRFEERMMIVQSYGFFEESMVKEEEPDLIITTV
                     PLKHNLNIPTVQVTLFINYEDESKVFQTLNILDKHRYHDDFVSLIKELMREDLFHVKN
                     TMNNPKEIIDYLCDELIEKGLATQSYKEDVFRREAVSATSFVNGFAVPHSIEVSANES
                     CISTLILDKPVIWGGFEVKLIILLAIRETDNHLLKIFFDWLSSIVSDSNKFTQLLEVR
                     EHQEFMEQVIM"
     gene            6366..6944
                     /locus_tag="PLK68_01005"
     CDS             6366..6944
                     /locus_tag="PLK68_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRM89018.1"
                     /translation="MATKVVKAKKERKIRYEDAPLVKRFISYLIDWYVGALCTAIPIA
                     IISQKLTNTMLNQNIVEFKQPYGIIAGILAVLFAIFYFVIVPAYIYPGQTLGKKICKI
                     KIVKVNNEQVTIKNMLLRQLLGVIVIEGVLYTASAIWHELVTIITQTNFVTPLMYAGF
                     IISGISILLYLFKGEHRTLHDYLGNTKVVLCK"
     gene            7031..7354
                     /locus_tag="PLK68_01010"
     CDS             7031..7354
                     /locus_tag="PLK68_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM89019.1"
                     /translation="MDGLELVSFKIISAVGMAKSSFIEAMKVAANGEFDVARAKIKEG
                     EESFNGGHLAHSELIQQEASGKHVVPSILLMHAEDQLMSAETIKVMALEIIRLNERVK
                     ALEKY"
     gene            7379..7699
                     /locus_tag="PLK68_01015"
     CDS             7379..7699
                     /locus_tag="PLK68_01015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM89020.1"
                     /translation="MKKVYLFCSAGMSTSMLASNMQDVANQHNLPIKVAAFPHNKLEE
                     IISEDRPDCILLGPQVKYMYEETVEQFGSQGIPIAVIDQGDYGMMNGEKVLKSAIRLI
                     KANK"
     gene            7716..>9082
                     /locus_tag="PLK68_01020"
     CDS             7716..>9082
                     /locus_tag="PLK68_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM89021.1"
                     /translation="MIKQLEKILMPLAEAIGRNKYLVSIRDGFLVSTPLLIAGSIFLL
                     IANFPIPAWTEWLSSVVINSNTGETLAAFIEKPSGATFTIMAVFAVVGIAYSFARQMK
                     TDKIFGAAVAIMGWFLIMPYTVEGTVEMAGKEIPVSLTGIPTGWVGAKGIFVGIICAF
                     VAVHIYSWVEKKGWVIKMPAGVPPTVVQSFAALIPATVVMTFFFAINLLFGFLGTNVF
                     QIIFEFLQTPLLNLGDTLGAMIIAYIFLHLFWFFGVNGGSVVGAVFNPILQTLSVENI
                     EFFKTGVGEGHIICQQFQDLFATFGGCGSTLSLIIAMLLFCKSKRITELGKLSLVPGI
                     FGINEPIVFGLPIVLNPTMIIPFILVPTINIIISYLAMSAGLVPICSGINIPWTTPVI
                     ISGFLATNWVGAILQAVLLVLGIFIYMPFIKIMDKQYINDEANAVHEAEEDDFSLDDL
                     SFDDL"
BASE COUNT         3161 a         1255 c         1590 g         3076 t
ORIGIN      
        1 atcaaattct ttagctgaaa atgagtcatg atgactatat tcgcgtgaca gtttttggat
       61 acagtaacta aaattatatg cattttcttc tgcttcgtta tttattttaa acggatcatc
      121 atcaggggtc gatagtttat agtaaccgtc atcttgagtt tttgtataat tttgcttaaa
      181 gaaattttct aaatagttat atattttaat gtttgaatta taataataac cattccagtc
      241 ttcatctgat ggaatctgaa tatctagaaa tggtgtatat agatttgtga gttcgcaagt
      301 aatggtagca gtagagtcat tagagatata tacttcgtca ttaattgcaa agtaaactaa
      361 attacttacg ataatatggt tatcggtaat tgcagaataa ttaaaagtta cattgattgt
      421 tttagtctta tcttcatcca tcaaggcaat aagtttttca catattgatt ttaatttttg
      481 atagctgatt tcactgagtg aaacctcagc aatattatca ccatttttag cagcaaagtg
      541 ccatctttca tgaggggcta agaatggttg aaaatcttca tcaaaggcta gataaactga
      601 tgtgacgttt cgatcaatat tagctataac aacatctgta aaccgtgaat agttatattt
      661 taaatcatca ttaatatcat cagcaacata tttagttggc cagtcatctt ttattgttgc
      721 tttatgtgca taatagccaa agtaagtgaa gattgtagta atcatcatca tggtgataat
      781 atagatactt gttattttgg taaatttgtt tagtcgtaac atgattgatt tcccctttca
      841 tcatatagta atcaaattta tcaagtaaat taataatttc cccttttttt aattttatca
      901 ttattttgct ttaaagactt gtaagataat aaaaaagcag actttagtct gcttgattat
      961 ggcatttaga cttgaaaata atgccaccaa taacgaacat acatgcaatc accacaactc
     1021 cgtattttga attatctgta atttgagtag taatccccat taataaagtt cccataaatg
     1081 aggctccctt accaaatata tcaaaaattc caaaatattc actcgcctga ctttctggaa
     1141 tgatttgagc ataatatgat cgcgataatg cttgaattgc gccttgaaaa acagcaacaa
     1201 aaacagctaa tacccagaat ttccaagctg tatcaagcca taaagcaaag acagcgatga
     1261 aaaagtatcc aataatgcaa gaaaggatca atgatttaac agggaatttt ttagcaagtt
     1321 ttccaaaaat caaagaaaat ggaaaagcta ctacttgagt aagtaataaa gctagtagta
     1381 aactattatc atcaattcca acatcttttc catatgaagt tgccatctca ataatagtat
     1441 aaacaccatc aatatagaaa aagaatgcga taaggaagaa gagtaccttt ttattatttt
     1501 taatactagt tagaacacgt tttaaattat taataattgg ttctttagcg gttgtataat
     1561 taatttgctg atagtttttc aataatggga tagtaataaa aaaccaccac aaagcattca
     1621 tgataaaggc aattgctgtt gccataacag ttgaaaggcc aattgtatca caacctaaaa
     1681 tcaatagtag actgattgta aaaggaatac aactaccgat atagccccaa gcatatccgt
     1741 gtgaagaaac ttgatcaacc cgctcatatg tcgtaatatc aaccagcata gaatcgtaaa
     1801 agatcaaact ggtcgcaaac cccgttttag caacgataaa aatagctaaa aatacaatcc
     1861 agccaactgg aatagaaagg gcagctaagc ttaatactcc catcatcata aagaatgtaa
     1921 agattggttt gcgcacacct ttacgatcac caatacttcc cagcataggt cctgctaatg
     1981 caacaatcaa tgttgaaatt gaaatagcat agctgtagta agcagtagaa tcacttaaag
     2041 atattccggc ttcagtagct aagtttttaa aatagattgg aataatagtt gatactaata
     2101 atacaaatgc tgaatttccg acatcatata aaacccaata cttttctaat tttgttagtt
     2161 tattcatagt tgtaattgaa tcgttttgat attgcagtat tgttctggta actgtaataa
     2221 tattcgatta tctcctttgt ggcaatattt atcgactggt taaaaagatt aactaatata
     2281 tcaatgtttt cttcaattct tttttctggt tttgcagtca tgaccattcc acagtatgac
     2341 ttagcattaa caattttcat tcctgttaga atcaaagatc tttttaaaac actccggcaa
     2401 gcaaaaagat gattaaagcg tttaaatgat tttaaagaag ctaagatcgt ggctggagct
     2461 aaattaaaaa acggagcaat aattttagca ttttcaagat tgatctggta atgttttggg
     2521 gttgaatagt taaatgattc atggtttagt atcataaatc gctgatctaa atctcgctcg
     2581 atttcaaagt gaccaatttc acgattaatc attgttttat cgatgtaacc atgacattca
     2641 ctatctaaaa taaagtgatt aataaaaccc aaaatatagg ctaatgattt ttcgtttggt
     2701 tgttctttta gaagtttcat cgcattttca aagaaagccc gtgcaatatc ttggtgtagc
     2761 tgattaccta aagtattgac aggatttttt ttaagcggtt gatagaaaaa taaaatatca
     2821 gggccttgaa gaccgatatt gtagagattt aaattatcat taataatttt tttaattgtt
     2881 gtattttcaa gcttatttaa aacttgattc ccaaattgct gatgtgtata acttgccggc
     2941 atagttaacc tcccattctt ggatattata taaagatatt atttgttgaa cgagcatgat
     3001 tagtttaaga ttaggtaaat atttatctaa gaaatctttt gattaaatta tactgtaatt
     3061 cgaagaataa ttacaattta ttaataataa aactgttcat ttataatgaa cagttcgata
     3121 gattatattt aaaaagcaat aattttattt gaaattatgt tcaaatcgat atgatagaga
     3181 acgatcatac ttaattacat caataaaatt gtagattgct tcatatgatt cactaatact
     3241 ttgattaaat aaactttcta acttttctaa gtatgggtcg actaatttat ttggtctgta
     3301 atttacaaca aacccctgga atcgtttata tgtaaacgtg atttttaaaa ctaggtaaat
     3361 caagccccgt ttataaaact gtggtgctaa taataaacga tcatagaact tcatcccttt
     3421 aagactctta tagagttctt tttttgttgc tttaaaatat ggctcaatgt tgttgagcgt
     3481 atgatcatta accttaatgt gatcagttaa tttagttctt agaggatcta aacgatcttt
     3541 gaccataaaa taacgatcga gctcagtttc tatttcaaaa tgagttacac cagtttcttt
     3601 gataatcgta ttaatatacg ggtggcagtt actatcaaga atgtagtgac agatgaaacc
     3661 acaaagataa gcaaattgtg gggcactttt ggtttctttt aagatttcta aagcttggta
     3721 aaaaaagatg tcagctcttt ctttatgcat agcactacct aaacgattga ttttactatg
     3781 gatatatggg cggttataaa aaagaatatc aggaccatgt aatccaatat caaatagatc
     3841 ttgattagtt tccaagacac gtttaagggg ctgatttaat ttatcaagca ctagttgtcc
     3901 aagacgataa tgactataag ctgccggcat tattttagat tatcgtattc attggcttta
     3961 aaaccaacta atacactatc cttagtttca acaattggtc tttttactaa catcccatca
     4021 gtagctaaca attctaattg ctcatcttca gtcattgtgg gaagtttatc ttttaattgg
     4081 agttctttgt attttaagcc agaagtatta aagaagcgtt tgagcggtaa gccgcttttt
     4141 tgatacaaag cagttaattc ttctttgctt aaacgttctt caacgatatg tcgatcatgg
     4201 tgatcaatcc caagttcatc aagatatttc ttggccttct tacaagtact gcattttgga
     4261 tattcaataa aatttatcat attctcacct cgtctatagt ttaactcatt ttatcctatt
     4321 ttaacaatgt tatgcataga aaaataagtt ataatttaac ccggatattt ttcctaacag
     4381 ttaggaaaaa agattgcaac ttaggaaaat gaatgcgtga tatattataa aaggagctga
     4441 taaagaaatg ctaaacaaac gccaaacaaa tattattgat cttttaaatg attatggaaa
     4501 atggattact ggtaaagaaa tcgcaaaagt tctaaatgta tctgaccgaa caattcgttc
     4561 agatatagag aatattaata attattatga ttgtatactg attcaggcta ataaacgtct
     4621 aggatatcgt ttagatgaga cattattgca taagcaggat attgaaacta aagatataat
     4681 cccacaaact tcccaagaac gttgtatata tattattcaa gaacttttgt ttaaaagtca
     4741 tgaaattaat ttgattgctt tgcaggatca ggtttttgta agtggttatt caattgataa
     4801 tgatataaaa aagatccgta aaatgattaa tgattaccct agcttaaaac tagttagatc
     4861 taaaaattat attagtttag aaggaaatga aacagataag cgaaagttat acaaacagtt
     4921 attgacggca gagacacaag ggaattttat gaatttaaat tcaattgcag gattatggaa
     4981 tagttttgat ctattagaag tgaaagatat actagaagaa atttgtgaaa aatatgatta
     5041 tcagattcat gagatgactt ttccaatgat catgattcat gccggagtgg cgattgagcg
     5101 gatcattaat cataactata ttaagaatca aacaattagt gaaaaattag aaagcagtcg
     5161 agaatatcag atttcatatg acttctttac acaggttagt acgatgatca atattgaact
     5221 tgttactgat gaagtaattt tatttgcttt attattaatg ggaaaaaggg caaatgatta
     5281 tcgtaaggat attatcaaag aaaagctggc agtcgatgtt gaacatttag ttgaagcagt
     5341 aattgttgaa gttaaggaat attttgatat tgatttttca aaagataagg atttaaaagt
     5401 gggtttagca atgcacttac aatcattgtt agagcgtcaa aaaaataatg ttcaagtaac
     5461 taatgtctat cttcaagaaa ttaaacgtaa gtatccttta gtatttgaaa tggcagtgcg
     5521 tgcaggcgag gtattagctg aaacttgtga ggttaatatt aatgaaaatg aattagcgtt
     5581 tttagctttg catttaggag cagcgtacga tcgggtcaat tctttaaaac gctatcgtgc
     5641 gattatgatc attcctaata atcagatgct atcaaagatg tgtcgggaca agttaaaaat
     5701 tcgttttgaa gaacgaatga tgattgttca aagctatggc ttttttgaag aatcgatggt
     5761 taaggaagaa gaaccggatt tgattattac aacagtacca ctaaaacata atttaaatat
     5821 cccaacagtc caagtaactt tatttattaa ttatgaagat gaaagtaaag tatttcagac
     5881 actaaatatt ttagataagc atcgctatca tgatgatttt gtttcattga tcaaagaatt
     5941 gatgcgtgaa gatttatttc atgtaaaaaa tactatgaat aatcctaagg aaatcattga
     6001 ttatttatgt gatgaactga ttgaaaaagg gttagcaaca caaagctata aagaagatgt
     6061 ttttaggcgt gaagcagttt ctgcaacttc ttttgtaaat ggatttgcgg taccacattc
     6121 aattgaagta tcagcaaatg aatcatgtat atcaacttta attttagata aaccggtgat
     6181 ttggggtggt tttgaagtga aattgattat tcttcttgca atcagagaaa cagataatca
     6241 tttattgaaa atcttttttg actggttgag tagtattgtc agtgattcaa ataaattcac
     6301 ccaactgtta gaagttcggg aacatcaaga atttatggaa caagtaataa tgtaaggaga
     6361 gaaagatggc aactaaagta gtaaaagcga aaaaagaaag aaaaataagg tatgaagatg
     6421 ctccattagt aaaacgattt ataagctatt taattgactg gtatgtcggg gctttatgta
     6481 ctgcgattcc gattgcgatt atttcacaaa agttaacgaa tacaatgtta aatcaaaaca
     6541 ttgttgaatt taaacagcca tatgggatca ttgcaggaat tttagctgtt ttatttgcaa
     6601 ttttttactt tgtaattgtt ccagcttata tttatccggg gcaaacgctc ggcaagaaaa
     6661 tttgtaaaat caaaattgtt aaagtgaata atgagcaagt aacaataaaa aatatgctat
     6721 taagacaatt attgggagtt attgtaattg agggtgtttt atatactgca agtgcaatct
     6781 ggcatgaact ggttacgatt attacccaaa caaattttgt tacacctttg atgtatgcag
     6841 gatttataat tagtggaatc agtattttac tttatttatt taaaggagaa catcgtactt
     6901 tgcatgatta tttaggaaat actaaagtag tgctatgtaa ataatttcct aagtctagga
     6961 aagatgacta gcaacaataa aaaaattaat tacttataat gttgattgta agggtattgg
     7021 aggataaaaa atggatggtt tagagttagt gagttttaaa attatatcag ctgtaggaat
     7081 ggctaaatca agttttatcg aagcaatgaa ggttgctgct aatggtgaat ttgatgtagc
     7141 tagagcaaaa atcaaggaag gtgaagaatc ttttaacggt ggacacttag cccatagtga
     7201 attgattcaa caagaagcta gtggaaaaca tgttgtacca agtattctgt taatgcatgc
     7261 cgaagatcaa ttaatgtcag ctgaaacaat taaggtcatg gctttagaaa ttattcgttt
     7321 aaatgaacgg gtcaaagcat tagaaaaata ttaacaataa caaagggagg acaaagaaat
     7381 gaaaaaagtt tatctatttt gtagtgcggg aatgtcaaca agtatgttgg caagtaatat
     7441 gcaggatgtt gctaatcaac ataatttacc tattaaagtt gctgcctttc cccacaataa
     7501 attagaggag attatctcag aggatcgtcc agactgtatt ttattaggac cacaagtgaa
     7561 atacatgtat gaggaaacgg tagaacaatt tggtagccaa ggaattccta tagctgtaat
     7621 cgatcaaggt gattatggca tgatgaatgg tgaaaaagtt cttaaatcag cgattagact
     7681 gataaaagca aataaataaa aaggagggta aaagaatgat taaacaactt gaaaaaatat
     7741 taatgccttt agctgaagct attggccgta ataaatattt agtatcaatt cgtgatgggt
     7801 tcttagtatc aacaccgtta ttgattgccg gttcaatttt cttactgatt gcaaattttc
     7861 caattcctgc atggacagaa tggttaagtt cagtagtcat taattcaaat acaggggaga
     7921 cattagctgc ttttattgaa aagccatcag gagcaacctt tactattatg gcagtctttg
     7981 ctgtagtagg gattgcttat tcatttgctc gtcagatgaa gactgataaa atatttggag
     8041 cagcggtagc tatcatggga tggttcttga tcatgcctta tacggtagaa ggaactgtag
     8101 aaatggcagg taaagaaata ccagtatctt taacaggtat cccaactgga tgggttgggg
     8161 caaaaggtat ttttgttgga attatctgtg catttgtagc tgtgcatatt tattcatggg
     8221 ttgagaaaaa aggctgggta attaaaatgc cggcaggagt accgcctact gtcgtacaat
     8281 cgtttgcggc tttaattcca gcaactgttg taatgacttt cttctttgca attaacttat
     8341 tatttggttt tttaggaaca aatgtgtttc aaattatatt tgaattctta caaacaccat
     8401 tacttaattt aggagatact ttaggagcga tgattattgc ttatatcttc ttgcatttat
     8461 tctggttctt cggagtaaat gggggttcag tagttggtgc tgtatttaat ccaatcttac
     8521 aaacattgtc agtagaaaat attgaattct ttaaaacggg tgttggtgaa ggacatatta
     8581 tttgtcaaca attccaagat ttatttgcaa ctttcggggg ttgtggttca acattatcat
     8641 tgattattgc aatgttatta ttctgtaaat ctaaacgaat tacagaatta ggaaaattat
     8701 cattagtacc agggattttt ggaatcaatg aaccaatcgt atttgggtta ccaatcgtgt
     8761 taaatccaac aatgattatt ccgtttattt tggtgccaac aatcaatatt attatttctt
     8821 atcttgcgat gtctgcgggg ttagtaccga tttgctcagg gattaatatt ccatggacaa
     8881 ccccagtaat tatttctgga tttttggcaa ctaactgggt tggtgctatt ttgcaagcag
     8941 tcttactagt attaggaatc tttatttaca tgccgtttat taaaattatg gataagcaat
     9001 atattaacga cgaagctaat gcagttcatg aagcagaaga agacgatttt tcattagatg
     9061 acttatcatt tgatgattta ta
//
LOCUS       DAOMTA010000017         8942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_70_length_8942_cov_2.700011, whole genome shotgun sequence.
ACCESSION   DAOMTA010000017 DAOMTA010000000
VERSION     DAOMTA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_8942_cov_2.700011"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..932
                     /locus_tag="PLK68_01025"
     CDS             171..932
                     /locus_tag="PLK68_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89022.1"
                     /translation="MNVFVTSLLKIINNPDIQDTNYNIAYYLLLHYYDVFEMTLQEIA
                     DACYVSVSTLNRFFRIFGFKKFSIIKDLMQVHAAARISQLEERSIRKNNQQVNQLLKP
                     LLDNDDYWTVSDQGLINQCCEMIKKCSRVILIGSNEMMDSLLRFQGDMVMMQKLVIQN
                     TIYNNNYIEPQADDLIILLSMTGRIAELKPTLIEHLLVGKNNLICVGYKNFLCEKALF
                     LKIPSYLDETLENMILDNYFQNIAYCYYGGYYDNR"
     gene            919..1608
                     /locus_tag="PLK68_01030"
     CDS             919..1608
                     /locus_tag="PLK68_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89023.1"
                     /translation="MIIGKLNLILCTSSYNSNDYLLAAYLIKKSNQIESIKMVDILKD
                     TKVSKSTLLRFLKKLGYKNYTDVQYLITKEKKHQEIFKERIIEKNLRDKLAKKQRLIV
                     IGDSYSVSSLIVYKQKFYEIGIDLDIQLSLKSYGQMVVEKKFTGSDLIIVVSLYKSDL
                     DLMAEFFSGYLELKHFIKEQKIDHLYVGKIATGNRNLDECYRIDDNINFSEAIYQLCS
                     LFEKIYNFYAL"
     gene            1880..2635
                     /locus_tag="PLK68_01035"
     CDS             1880..2635
                     /locus_tag="PLK68_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRM89024.1"
                     /translation="MYLAHGGGGNDVEWMNIASANNIMDNLIADGEVADAIVVTKDNT
                     YFNFEKGDSLKNLTECIMLYIEKNYSVSANSQDKVLAGLSSGATVTVQAMFYSNETFG
                     YYGVFSPSRTLDFVEETLTLEMVAAFPKVSQYYVSVGIFDIFVRRNVNVQINEELIKA
                     GANVVFEWKNGAHDWGVWRSQFSEFVKDYLWDRETVVSTPSNPDADVAPQSNSTAAVK
                     TGDSVDMTGLLIISGLSLLGISIQYYYRHRKTH"
     gene            3094..4377
                     /locus_tag="PLK68_01040"
     CDS             3094..4377
                     /locus_tag="PLK68_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRM89025.1"
                     /translation="MSKDNLRFETKQLHVGQEITDPTGARAVPIYQTAAYVFDNCDHA
                     AARFGLSDAGNIYGRLTNPTEDVFEQRMAALEGGVAALAVASGAAAVTYAIESITRSG
                     DHIVAAKQIYGGTYNLLAHTLTNYGVNTTFVDSDDPENFKLALQENTKAIFIESLGNP
                     NSSLVDVEAVSKIAHEHGIPLIIDNTFGTPYLFRPIEHGADIVVHSATKFIGGHGTTL
                     GGVIVDSGKFDWQASGKFPQLSKPDPSYHGVIFTEAAGAAAFVTRIRAVVLRDKGATI
                     SPFNAFLLLQGLETLSLRVERHVENALKVVDYLSKHPKVAKVNHPSLNNSPYHELYQR
                     YFSHGAGSIFTFEIAGDENDAKKFIDNLEIFSLLANVADAKSLVIHPASTTHSQMNEA
                     ELAASGIKANTIRLSIGLEHIDDLLEDIENAFDHV"
     gene            4504..4749
                     /locus_tag="PLK68_01045"
     CDS             4504..4749
                     /locus_tag="PLK68_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM89026.1"
                     /translation="MSELTKIPYVGKATAASLNLIGYETIASLKGADPEEMYQKECDV
                     RGQMVDRCQLYMYRMAVYYASSKNPDPKKLKWWIWKD"
     misc_binding    4792..5027
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5063..6547
                     /gene="lysS"
                     /locus_tag="PLK68_01050"
     CDS             5063..6547
                     /gene="lysS"
                     /locus_tag="PLK68_01050"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRM89027.1"
                     /translation="MEERKFSEQELVRREKLQELVDKGIDPFGQRFDVTAYSKEIKET
                     YGDKTHEELEEMAVEVKVAGRIMTKRRKGKVCFMHIQDRDGQIQLYVRKDAVGEDVYE
                     IIKKGDIGDIVGVKGTVFMTNTGECSIKVEEYTHLTKALRPLPEKYHGLSDVEERYRR
                     RYVDLIMNEEARKIAFARPKIIRSIQHYLDNQGYVEVETPVLNPILGGASARPFVTHH
                     NALDMNFYLRIATELGLKRLIVGGMDAVYEIGRLFRNEGMDRTHNPEFTTIEVYKAFS
                     DLEGMMDLTEGIISNAANEVCGTYELEYKGNKISLAPGFKRISMTDAIKEKTGIDFAD
                     QLSFDDAKKLAEEHHIEVEPHFGYGHIINEFFEKYVEETIIEPTFVYGHPIEISPLAK
                     KNLDDPRFTQRFELFICGNEYANAFTELNDPIDQYERFANQLKEKELGNDEANEMDLD
                     YVEALEYGLPPTGGMGMGIDRLVMLLTGQESIREVILFPHMKNK"
     gene            6745..7020
                     /locus_tag="PLK68_01055"
     CDS             6745..7020
                     /locus_tag="PLK68_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM89028.1"
                     /translation="MEKQFNQDAKVFKAFCDPNRLRILDILKSGEHCACKLLDILDVS
                     QSTLSHHMKLLTDAKIVNVRKDGKWSHYSLSKDGIQFAIEYLQQLKV"
     gene            7121..>7467
                     /locus_tag="PLK68_01060"
     CDS             7121..>7467
                     /locus_tag="PLK68_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRM89029.1"
                     /translation="MSQILLFFQDQILGMRWLNELIGSILTSIGLNIDEKIGGMLHFF
                     IYDSIKIFILLSVLIYIISYIQSYFPPEKTKKILGRFYGIWANILGALLGTVTPFCSC
                     SSIPIFMGFTSAG"
     assembly_gap    7468..7477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7478..8127
                     /locus_tag="PLK68_01065"
     CDS             <7478..8127
                     /locus_tag="PLK68_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRM89030.1"
                     /translation="VTFSFLISSPMVDLGALILLMSIFGWKVAVLYVIAGLIISVVGG
                     RLIEKLGMDDQVADFIKQTDNVDVGFVSLTVKDRRNFAKEQVIETVKKVSIYIFVGVA
                     IGSVIHNVIPEHWIQSILGDHNFYSVPLATVVGVPMYADIFGTIPVAESLLLKGAGLG
                     TVLSFMMAVTTLSLPSMIMLSKAVKRKLMIVFVAIVTVGIISVGFMFNIFSFLLM"
     assembly_gap    8331..8340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8410..8736
                     /locus_tag="PLK68_01070"
     CDS             8410..8736
                     /locus_tag="PLK68_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM89031.1"
                     /translation="MKLFKKNCCNVQCGDGSMKRGLEKKDKGSRIKVLGSGCAKCATL
                     EKNVKEALDELGQEMSIEHITDFTEIASYGIMSTPALVIDEKVVSFGKVLTIEEVKNI
                     ILTIDG"
     gene            8830..>8942
                     /locus_tag="PLK68_01075"
     CDS             8830..>8942
                     /locus_tag="PLK68_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HRM89032.1"
                     /translation="MNNMPRVAFICVHNSCRSQIAEAIAKLFASDVFESYSA"
BASE COUNT         3167 a         1032 c         1762 g         2961 t
ORIGIN      
        1 agagggctgg ctcaggcagg agcaaatgga atcatcgtag cacgtcatat tattgaacat
       61 cttaaatagt aaggtaaaaa tcctgtttta gacaggattt ttctattttg acaggtgtag
      121 tatcatggtg gtaaacttaa taataagacc atagggaagg agaaacttaa atgaatgttt
      181 ttgttacttc attattaaaa ataattaata atccggatat tcaagatact aattataata
      241 ttgcttatta tttattactg cactattatg atgtttttga aatgacactt caggaaattg
      301 cagatgcatg ttatgtttct gtttctactc tcaatcgctt ttttagaata tttggattta
      361 aaaaattttc aattattaaa gatttaatgc aagtccatgc ggcggcacga ataagtcagt
      421 tggaggaacg ttcgattcgc aaaaataatc aacaagttaa tcagctttta aaaccgctat
      481 tagataacga tgattattgg acagtatcag atcaaggatt aataaatcag tgctgtgaga
      541 tgattaaaaa atgtagccgt gttattttaa tcggttcgaa tgagatgatg gattcattat
      601 tgcgttttca aggtgatatg gtgatgatgc aaaaacttgt gattcaaaat actatttata
      661 ataataatta tatcgaacct caagcggatg atttaattat tttgctttca atgacaggaa
      721 gaattgcgga gttaaaacca actttgattg aacatttatt agtggggaaa aacaatctta
      781 tttgtgttgg ctataaaaat tttctttgtg aaaaagccct atttttaaaa attcctagtt
      841 acctagatga gacgctagaa aatatgattt tagataacta ttttcaaaat atagcttatt
      901 gttattatgg tggttattat gataataggt aaactaaact taattttgtg tacttcttca
      961 tataatagta atgattatct tttagcagcg tatctgatca aaaaaagtaa tcagattgaa
     1021 tcaatcaaga tggttgatat tttaaaagat acaaaagtat caaaatcaac tttattacgt
     1081 tttttaaaaa aattaggcta taaaaactac actgacgttc aatacttgat tactaaagag
     1141 aaaaaacatc aagaaatatt taaagagaga attattgaga aaaatttaag agataagtta
     1201 gcaaaaaaac aaagacttat agtaattggt gatagttact cagttagctc actaattgta
     1261 tataaacaaa agttttatga aattgggata gatttagata tccaattatc attaaagtca
     1321 tatggacaga tggtagttga aaaaaagttt actgggagtg acttaataat agtcgtatct
     1381 ctatataaaa gtgatttgga tttaatggca gaatttttta gtggctatct tgaattgaaa
     1441 cattttatta aagaacaaaa gattgatcat ttatatgtag gaaagattgc gacggggaat
     1501 cgtaatcttg atgagtgcta tagaattgat gataacatta attttagtga agcaatttat
     1561 cagctttgta gtttgtttga gaaaatttat aatttttatg ctttgtaagg ttcaaaatat
     1621 gccccttttt attatttatc aatgattttc tttattatag aaaccacgcc aaataaaagc
     1681 aatttcaaaa tgaggttgtg ataaaatatt aatagaaaag agaaaaggag gctagattta
     1741 tgagaagatt aaaattagga ataatcagct tattatgttt atcatttata gaggctaata
     1801 taacaacatt gatggcagta gccagccgtt aggtatatat ttaccgcatg actatgatac
     1861 aaataaaact tataagacaa tttatcttgc acatggtggt gggggaaatg atgttgagtg
     1921 gatgaatatc gcttcagcaa ataatattat ggataatcta attgctgatg gtgaagtggc
     1981 tgacgcaatt gtggttacta aggataatac atattttaac tttgaaaagg gtgatagcct
     2041 aaaaaatctt acagaatgca ttatgcttta cattgaaaag aattatagtg tttcagcaaa
     2101 ttcccaagat aaagtactag ctggtctgtc tagtggagct acagttacag ttcaagcaat
     2161 gttctatagt aatgaaacat ttggctatta tggtgtattt tcaccgagtc gtacattaga
     2221 ttttgttgaa gaaacattaa cactggaaat ggttgcagcg tttcccaaag tatcacaata
     2281 ctatgtgagt gtaggaattt ttgatatttt tgttaggcgg aatgtcaatg ttcaaattaa
     2341 tgaagaattg ataaaagccg gagctaatgt agtttttgaa tggaaaaatg gagctcatga
     2401 ctggggtgta tggcgtagtc agttcagtga atttgtaaaa gattatttgt gggatcgtga
     2461 aacagtagtt tcaactccat ctaatcctga tgccgatgtt gctccccaat caaattcaac
     2521 agctgctgta aaaactggcg atagtgtcga tatgacagga ttgttgatca taagcggttt
     2581 atcattatta ggaatttcaa tacaatacta ttatagacat cgtaaaacac attaataaaa
     2641 acttttaaat aattagatat aatctcgcca aagtagatag atgtgataat ataatcttac
     2701 caagactata gaagtgagat ttttattatg gaaaaaattg aaaggtatag caatatattt
     2761 tttaagtttt agaaagaaca aaattaaaga aagggaatca ttaataatat aagactattg
     2821 ctaccccata aaatgctagt ttatatctag taagttaatt atttttgacc atttgacaat
     2881 agatacatat aaatttataa catgactttt tatttcccta ttgacaggta ggaaatagtt
     2941 tagtatactc tattcatact ataacagtag gataagggga agaaaaatga attttgaact
     3001 actacaacag ctcagaaata attttcggaa tggacgaatg tattgttttt tataaatcat
     3061 agataacaaa cattaagaaa agaggaaaat aaaatgagta aagacaattt aagatttgaa
     3121 actaaacagt tacatgtagg acaagaaata actgatccta ccggagcaag agcagtgcct
     3181 atttaccaga cagcagctta tgtatttgat aattgtgatc atgcggcagc acgttttgga
     3241 ttaagtgatg ctggtaatat ttatggacgc ttaactaatc caactgaaga tgtttttgaa
     3301 caaagaatgg cagctttaga aggtggggta gcagcattag cagtagcttc aggagcggct
     3361 gcagtgactt atgcgattga aagtattaca cgaagtggtg atcatattgt ggcagctaaa
     3421 caaatttatg gggggacata taatttatta gctcacacac tgacaaatta tggtgttaat
     3481 acaacatttg ttgatagtga tgatcccgag aattttaaat tagcgttaca agaaaatacc
     3541 aaagcgattt ttatagagtc attaggaaat cctaattcaa gtttagttga tgttgaggca
     3601 gttagtaaaa ttgctcatga gcatggtatt ccattaatta ttgataatac atttggaaca
     3661 ccatatttat ttaggccaat tgaacatgga gcagatattg ttgtgcattc agcaactaag
     3721 tttattggcg ggcatggaac tactttaggt ggtgtaattg tcgattctgg aaagtttgat
     3781 tggcaggctt cagggaagtt cccacaatta agtaaacctg atcctagtta tcacggtgtt
     3841 atttttactg aagctgctgg agctgccgcc tttgtaacta gaattagagc agtagtatta
     3901 cgtgataagg gagcgactat ttctccgttc aatgcttttc ttttattaca aggattggag
     3961 acattatcac tacgagtaga gcgtcatgta gaaaatgctt taaaagtggt tgattattta
     4021 agtaaacatc ctaaagttgc aaaagttaat catccatcac taaataatag tccttatcat
     4081 gaattatatc aacgatattt ctcacatgga gctggatcaa tttttacttt tgaaattgct
     4141 ggtgacgaaa atgatgctaa gaaatttata gataatttag aaattttttc tttattagca
     4201 aatgtagctg atgcgaaatc attagtaatt catcctgctt caacaacgca ttcacaaatg
     4261 aatgaagctg aattagcagc atctggaatt aaagctaaca ctattagatt gtcaattggt
     4321 ttagagcata tagatgattt gttagaagat atcgaaaatg catttgacca tgtataaaaa
     4381 ataaaaatac gtcaataata taactagaaa ttgttgttgc ttaaaatttc tattgaccaa
     4441 aagctatccc agaggatagc ttttaagtta ttagtggctt ataatgtaat tgaggtgatc
     4501 attatgagtg agttaactaa aataccttat gttggtaaag caactgctgc atcattgaat
     4561 ttaattggtt atgaaacgat agcttcttta aaaggcgctg acccagaaga aatgtatcaa
     4621 aaagaatgtg atgttagagg acaaatggta gatcgctgcc aactttatat gtaccgtatg
     4681 gcagtatatt atgcaagtag taaaaatcca gatcccaaaa agttaaaatg gtggatttgg
     4741 aaagattgat attgacaata attggaagat aagttaagat tacaaagatg aataaaggcg
     4801 atgaatagag gagtaattgt attttatgtg tttaagagag tctcggctgg tggaatggga
     4861 taacacagga tataatgaaa aaagactagg agctgtatct aggatattaa ggttgattag
     4921 ataacgtgag ccacgttaca ggcaaaacta ttatttgata gttgttgagg ttaataaggt
     4981 gacttattaa cgaattaggg tggtaccacg atactctcgt ccctttagcg gatgagagtt
     5041 ttttatattt taggaggaaa aaatggaaga aagaaagttt agcgaacaag agttagttcg
     5101 ccgtgaaaaa ttacaagagc tagtagataa gggaattgat ccttttggac aacggtttga
     5161 tgttactgca tattcaaaag aaattaagga aacttacggt gataagacgc atgaagaatt
     5221 agaagaaatg gctgttgagg ttaaagttgc tggaagaatt atgacaaagc gtcgcaaagg
     5281 gaaagtgtgc ttcatgcata ttcaagatcg agatggtcaa atccagttat atgtacgtaa
     5341 ggatgcagtc ggtgaagatg tttatgaaat tatcaaaaaa ggtgatattg gagatatcgt
     5401 tggtgtaaaa ggaaccgtat ttatgacaaa tacaggggaa tgttctatta aggttgaaga
     5461 gtatactcat ttaactaagg ctttacgtcc tttaccagaa aaatatcatg gattaagtga
     5521 tgttgaagaa cgttatagaa gaagatatgt tgatttaatc atgaatgaag aagctcgtaa
     5581 aattgctttt gcaagaccaa aaataattcg ttctattcaa cattatctag ataatcaagg
     5641 ctatgttgag gtagaaacac ctgtattaaa tccaatttta ggtggtgcca gtgcgagacc
     5701 ttttgttact catcataatg ctttggatat gaatttttat ttacgtattg caactgaatt
     5761 aggattaaag agattaattg ttggtggtat ggatgcggtt tatgaaattg gacgtttatt
     5821 cagaaatgaa ggaatggata gaacacataa tcctgaattt acaacaattg aagtttataa
     5881 agcctttagt gaccttgaag ggatgatgga tttaactgaa ggaattattt caaatgcagc
     5941 taatgaagtt tgtggtacat atgagttaga atataaggga aataagattt ctttagcacc
     6001 aggatttaaa cgtatttcaa tgacagatgc aattaaagaa aaaacaggaa ttgattttgc
     6061 tgatcaatta agctttgatg atgctaagaa attagcagaa gagcatcata ttgaggtaga
     6121 acctcatttt ggctatggtc atattatcaa tgaattcttt gaaaaatatg ttgaagaaac
     6181 aattattgaa ccgacatttg tctatggtca tccaattgag atttcaccac tggcaaagaa
     6241 gaatttagat gatccacgtt ttacacaacg ttttgaatta tttatctgtg gtaatgaata
     6301 tgctaatgca tttactgaat taaatgatcc aattgatcag tatgaaagat ttgctaatca
     6361 gttaaaagaa aaagaattag gtaatgatga agctaatgaa atggatttag attatgtaga
     6421 agcattggaa tatggattac caccaacagg tggaatgggt atgggaattg atagacttgt
     6481 tatgttatta actggacaag aatcaatccg tgaggtaatt ttattccctc atatgaagaa
     6541 taagtaaaat catgtatttt cttgtgagaa tttcgtgaga atgaagcaga tttcttaaat
     6601 gatatctgct ttttatatgc atgaatctaa gaataatatc atttctttca agcactataa
     6661 ttacgatata ttttacaaat atattgacga atatcgatat gtatgttaat atcacatcga
     6721 agaagataga tatgaggtgg tttgatggaa aaacagttta atcaagatgc taaggttttt
     6781 aaagcattct gcgatcctaa tcgtttaaga atattagata ttttgaaatc cggtgaacat
     6841 tgcgcatgta aattgttaga tatattagat gttagccaat ctacgttatc gcaccatatg
     6901 aaactgttaa ctgacgctaa aattgtaaat gttagaaaag atgggaaatg gtctcattat
     6961 tctttatcta aagatggtat acagtttgca atagaatatt tacaacagct caaagtatga
     7021 aatatgtaaa taggttaact gttgtataaa ttaccaccac taaataggtg gaaatttatt
     7081 tgttttatac atcgaaatat atcgatataa ggagggcgct atgagtcaaa ttttgctgtt
     7141 ttttcaagac cagattcttg ggatgcgatg gttaaatgaa ttgattggat caatattaac
     7201 tagcattgga ttaaatatag atgaaaaaat aggtgggatg ttgcattttt ttatttatga
     7261 tagtattaag atatttatat tattatcggt attgatttat attatttcat atattcaaag
     7321 ttattttcca ccagaaaaaa caaaaaagat attaggaaga ttttatggta tttgggcaaa
     7381 tatattagga gctttacttg gaacagttac accgttttgt agttgttcat cgattcctat
     7441 cttcatggga tttacaagtg caggcttnnn nnnnnnngag tcactttctc atttttgatt
     7501 tcttcaccta tggttgatct aggagcgctg atattattaa tgagcatatt cggatggaaa
     7561 gtggctgtgt tatatgttat agctggtttg attatatctg ttgttggggg aaggttgata
     7621 gaaaaacttg gtatggatga ccaagtggct gattttatta aacagacaga taatgtagat
     7681 gttggttttg tgagcttaac agtcaaagat agacgaaact ttgcaaaaga acaagtcata
     7741 gaaacagtta agaaagtatc aatatatata tttgtaggtg ttgctattgg atcagtcatt
     7801 cataatgtaa tacccgaaca ttggatacag tctatactag gtgatcataa tttttattct
     7861 gttccacttg caacagtagt tggtgtacct atgtatgctg atatatttgg aacaattcct
     7921 gtagcagaaa gtctattgtt aaagggggca gggcttggaa ctgttttatc atttatgatg
     7981 gctgttacaa cattatcttt accatcaatg attatgctat caaaggcagt gaagagaaaa
     8041 cttatgattg tatttgtggc aatagtaaca gtaggtatta tatctgtagg ttttatgttt
     8101 aatatatttt catttctatt aatgtagaag atatgtttaa tatgagaaat attgtacatt
     8161 ttaatataac gatggagatt aattaaatgt taaaaagtgg ttttaactat tcaattcact
     8221 cagttggtgg atgtacatgt ttggggttat aattaaggta ggacgggaaa gaagattttg
     8281 ttaaagaaat tattttagtt ataaatcagt atctagatga taaatggatg nnnnnnnnnn
     8341 cctagataag aaaatccatt attagatatt gtctcaaaat ttaattatga ataaaaggag
     8401 agataagtga tgaaattatt taagaaaaat tgttgtaatg ttcaatgtgg tgatgggagt
     8461 atgaagagag gattggaaaa gaaagataag ggttcaagaa ttaaagtctt aggttctggc
     8521 tgtgctaaat gtgcaacttt agagaaaaat gttaaagaag ccttggatga attaggacag
     8581 gaaatgagta tagaacatat cacagatttt acagaaatag caagctatgg gattatgtct
     8641 acacctgcgc ttgtaattga tgagaaagta gtatcttttg gaaaggtttt aacgatagaa
     8701 gaagttaaga atatcatatt aacaatagat ggatgaatca ttgaaacaag gtgttttttg
     8761 tgaaggggat ttaagcgaga tgaaattgct tattattgtt tttatttaat attaaacaat
     8821 ttgaaagaaa tgaataatat gccaagagtc gcatttatat gtgttcataa ttcttgtaga
     8881 agccagattg cagaggcaat agcaaaattg tttgcttctg atgtatttga aagctattca
     8941 gc
//
LOCUS       DAOMTA010000018         8674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_76_length_8674_cov_2.317090, whole genome shotgun sequence.
ACCESSION   DAOMTA010000018 DAOMTA010000000
VERSION     DAOMTA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76_length_8674_cov_2.317090"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2321
                     /locus_tag="PLK68_01080"
     CDS             <1..>2321
                     /locus_tag="PLK68_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89033.1"
                     /translation="GKVELTDSKAGLITNTNTDLKAIKLEGFDDSKTSTLKISENKTI
                     KLINPTIYDVKIESSNPKVVKYENNQIIGVSNGTAYLKGIVPGELEVFRYEIKIGTGE
                     NLDISPKYTKPADDTGDSRYIKQGNGEETDNLITVDPDNIIVKDYMGGAMELEMIEWF
                     DMDDARWERYFQRIQYMELGFVRCMLAAYWYCTGFDDHNNPIYVWDCLNEDGSPKYYS
                     RDGYPIDPLGNNIEPNIKRMKRLYQLFDFFKKNNITVMTGEWQYPEAAEWYYVPGENL
                     KQYNLTLDDPRYAQIAADFIEHMVKDKNYTCIEQYDLGNEVNINPGSYQYDKWKQANL
                     NLYDQLKQRGLEKTISLTPDLSYWLDLWYNQSIADLHDVFEQYEFHWYVNEVNLKNGV
                     FENEMRMLVNNAAGQDNPDKKVYMGEVGMRDYLNNVDQVTNIDSFEYGLWMANILVQG
                     TRAGMSGLTAWSLDDSVHINGPMTYDYSVDQKSLMKVWGLWNSLGEYQGVSEDIRPWY
                     VPWALASKFMPKGSQIIYTSPTEQQVYSTAAINGDDLTISVVNHSGKEQQVLVKIPEA
                     TKKVDLSVYAYFKDDLNQDENGYPVRSDFKKNLDLQEGIVLTLPADGNLVLTTMEGEN
                     KFNSKDSGYFSDSMQSLQNTVDYQNIGFNSYEKHPNNAQNRINMNQDIFGDVTRINRA
                     GKDKASLVYQRAEGIKNFRIHSYVKGAVGSNFKVYGSNDKNEWTEIKINEIEKYSLTK
                     GWKFAVLTPESEIEYKYIKIELGNEVSSTDIQV"
     assembly_gap    2322..2353
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2354..4319
                     /locus_tag="PLK68_01085"
     CDS             <2354..4319
                     /locus_tag="PLK68_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM89034.1"
                     /translation="SEPLKLEIPDDAQSSEYKEIMKDVQLATNDIMSIYSISGFENLP
                     ENCTLKSSDENIVKITDMSFKAVNSGSALVNVYRDGKIIGSIIVNVYAFHDSVEDGNG
                     LVYQLNNMKYDLMADANDTTFSRTNDGGNVIYKLAGIKDFSIRGYVADSNDKLFEQIK
                     IYASKDGNTWESIEIKVDGINALSNPYWHYANIIPQEAITKDYDYLKIAIEGTLTDHW
                     KIQVGDVRIQDKPFNSVVNKIELANQDLKLAVGNSQIIGAKVYPEAVFPFKVIFDAAD
                     NEIVSVDKDGLVTGLKAGKTTVRVYSQDNPEIYQDCTIEVTEVIDSSKEIADSIKELT
                     IINNQIVLPVVEGYEITIKSSSIPNIIDLDGKVTPSNQDEQVALVLAVKNKATNVVSD
                     TANIIVTVAKKDETVMIEFDITDVVLKVYQEFDPLADIKVFENDTDITKNVRIISNNV
                     DLNHHGKYFVTYEVTDSKNITHSHTRNVEVRGIKSISCPEYQVDLTSLKDLDPDVFLN
                     IVKVDKQVILDKLNSKLIDDQIIISCYDLAMMLNNKEYVLTDNQLTIVWNVTSKMQGF
                     KVLMLDKDGNVVEVPMTVNGKEITFMVEQLGTFAIVGEQKVETKEPDENKPENKVETA
                     VKTGDEVYYAEYAVLGLLALSLVYGMKKRG"
     gene            complement(4358..5224)
                     /locus_tag="PLK68_01090"
     CDS             complement(4358..5224)
                     /locus_tag="PLK68_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89035.1"
                     /translation="MSLLRKLEMAVDFSTSEKLIATYILKNGPDVLNMSTSQLAKATY
                     TSPATITRLCQKLEYKGYNDFKIALSAQLQYTFDRNSETNPNFPFKKRDTIHTIKHSI
                     ATLAKDSINLTVNNIDNDILAKIIIMLDQTKVIDIYGVSGPLRIASDFQYKMFRIGKN
                     VQIPPMVKEQLFQAAQSNKDHCAILISYSGETNEVIAAAELLKRRKVPMIAITSFGES
                     RLSKLCDYVLFLDSRERIYSKVSTFGSTISIHIMLDIIYSCLFARHYDENLELKLETD
                     HIIDHRHIKQIK"
     gene            5607..>8674
                     /locus_tag="PLK68_01095"
     CDS             5607..>8674
                     /locus_tag="PLK68_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM89036.1"
                     /translation="MNRKFKRFLTSLLSLAVVASSGIGPVKAVDNTPKLDSKVYINSD
                     NVLTKDFEGFGVQWDPSDLFDYTDEQWSSFYEKASFLSPNVMRVMLHDGDSYCIGFED
                     DGTPIYDWESVMMKRVYKILDFAQQNDIPIMLGEWRSISERGYLSYDDHGKTVNWSSP
                     TWARMIGDCLEHLIVDKGYTCIKYYNMINEPNYYKRDHGDVTNEYVYDQWKQAITNLR
                     NEMDSSGIEKIENIKIVGPDVYDSQEAWINQATSDEMKDKIELTEVHRYAPQSEVESG
                     LIEKKLKTWKEQAESLDPEVAEEGFALGEMGISGTGPGDSQLNARKYDYGVDIFDYGV
                     QAIRAGLKFGSVWGFEDSMHVQHNDIVNNFKDQYGPAATTEEGRDYVVHTPTGDPSID
                     NDIKIWGFWNELGEEMAAQNAEHNVTGRANTVKASDENLKPWYYTWSMLCRYFPSGTK
                     ILETTDSYVDQLRVTSGIIPVADDKGDISIAVVNNSSSQKTLEVNMPNAASKVDLNQY
                     FYYDGEIDGKTRPVNEKGQLLQYDTIENANLKDGVNVTLPAKSCMILTSLGYEGESHP
                     MSFTTGQTTDVEKVEIYETTNANQLEVGKSYQLAANYIPSISKGEMEWSVADYFGNAS
                     DKALIDENGLLTVKKAGQFKVIGNLKGKPEIQDTLVFKATSSSILVDELNDLENGVAL
                     SYQDIIKDDNSANFNGDKTVKRSDSNANGKPGIITYQANNIYDFEFSAYSLNNNLDKS
                     GNFVVEVSQNGDSWSPVECEFIQGSKLSSGWYPYTIKNKAVIKDDGYQYLRVTITSKS
                     GYKTYDPQYAGGSIYYDYQGASQIDIQSHNEFIVKGQELQFKAEVLPSIASQEVSWKV
                     LSLEGKPTELATISPDGILTAKAKGEVVVVATAKDTDVSAYSHINIINGYFVDEIEDF
                     SKMYQYGEFAFDDPKSSNFTDKTRIKRLSDSNQSIVYALSDIEKATFEIYKNGSFVND
                     SVDIYASSDGLTYNKVEKNVVNAGKAASSTEYYLYEVSTKELGDNVNYIK"
BASE COUNT         3163 a          973 c         1712 g         2794 t
ORIGIN      
        1 tggtaaagta gagttgacgg atagtaaagc tggattaatt actaatacaa atactgattt
       61 aaaagcaatt aagttagaag gatttgatga tagcaagact tctacattaa aaatatcaga
      121 aaataaaaca ataaaattaa ttaatccaac aatttatgat gtaaagattg aaagtagtaa
      181 tcctaaagtc gtaaaatatg aaaataatca gattattggt gttagtaatg gaactgctta
      241 tttgaaaggg atagtacccg gggagcttga agtatttagg tatgaaataa agattggaac
      301 tggtgaaaat ttggatattt cacctaagta tactaaacca gctgatgata caggtgatag
      361 ccgttatatt aaacaaggaa atggtgaaga aacagataat cttattactg ttgatcctga
      421 taatattatt gtaaaagatt atatgggtgg agccatggaa cttgagatga ttgaatggtt
      481 tgatatggat gatgctcgtt gggaacgtta ttttcaaaga attcaatata tggagttagg
      541 ctttgtcaga tgtatgttag cagcttattg gtattgtaca ggatttgatg atcataataa
      601 tcctatttat gtttgggatt gtttgaacga agatggtagt ccaaaatatt attctcgtga
      661 tggttatcca attgacccat taggaaataa cattgaaccg aatatcaaaa ggatgaaacg
      721 tttatatcaa ttgtttgatt tctttaaaaa gaataatatt actgtaatga caggtgaatg
      781 gcaatatcct gaagcagcag aatggtatta tgtgccaggt gaaaatttaa aacaatataa
      841 tttaacttta gatgatcccc gttatgctca aattgcagcg gattttattg aacatatggt
      901 aaaggataaa aattatactt gtattgaaca atatgattta ggtaatgagg taaatataaa
      961 tcctggatca tatcagtatg ataaatggaa acaagcaaat ttaaatcttt atgatcagtt
     1021 aaagcaaaga ggtttagaaa agacaatttc gttgacaccg gacctaagtt actggttaga
     1081 tttatggtat aaccaaagta ttgctgatct tcatgatgtt ttcgaacagt atgaattcca
     1141 ttggtatgta aatgaagtta atttaaaaaa tggagttttt gaaaatgaaa tgcggatgtt
     1201 agttaataat gccgctggtc aggataatcc tgataaaaag gtctatatgg gtgaagtcgg
     1261 aatgcgcgat tatttgaata atgttgatca agtaacaaat atagacagct ttgaatatgg
     1321 attatggatg gctaatattc ttgtacaagg aacaagggcg ggaatgtctg gattaacagc
     1381 ctggtcacta gatgattctg tgcatattaa tggtccaatg acttatgatt attcagtaga
     1441 tcaaaaatca ttgatgaaag tctggggact atggaattca ctaggtgaat atcagggagt
     1501 atcggaggat atacgaccat ggtatgtacc ttgggcatta gcaagtaaat ttatgcctaa
     1561 agggtcacaa ataatttaca ctagtccaac tgaacagcaa gtgtattcaa cggctgccat
     1621 caatggagat gatttaacaa tatccgtagt aaatcattca ggtaaagaac agcaagtgtt
     1681 ggtaaaaatt cctgaggcga ctaaaaaagt ggatttaagt gtttatgctt attttaaaga
     1741 tgatttaaat caagatgaaa acggatatcc tgttcgttct gattttaaaa agaatcttga
     1801 tttgcaggaa ggaattgttt taactttacc agctgatggt aatctggttt taactactat
     1861 ggagggtgaa aataagttca atagtaagga tagcggatat tttagtgact caatgcagtc
     1921 attacaaaat acggttgatt atcaaaatat tggctttaac tcatatgaaa aacatcctaa
     1981 taatgctcaa aatcgaatta atatgaatca ggatattttt ggggatgtga caaggattaa
     2041 tcgtgccggc aaagataaag cttctttggt atatcaaagg gctgaaggaa ttaaaaattt
     2101 tagaattcat agttatgtta aaggagcagt tggaagtaat tttaaagttt atggttcgaa
     2161 tgataaaaat gaatggacag aaataaaaat aaatgaaata gagaagtatt cactaactaa
     2221 gggatggaaa tttgcagtat taactccaga gtcagaaatc gaatataaat atataaaaat
     2281 tgagttagga aacgaggtat caagtacaga tattcaagtt gnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnncagtgaa ccgctcaaat tagaaatacc tgatgatgct caaagtagtg
     2401 aatataaaga aattatgaaa gatgttcaac ttgctacaaa tgatataatg agcatttatt
     2461 caataagcgg ctttgaaaat ttacctgaaa attgtacgtt aaagagcagt gatgaaaata
     2521 ttgttaaaat tactgatatg tcatttaagg cagttaattc aggtagtgct ttagttaatg
     2581 tttatagaga tggaaaaata attggaagta tcattgtcaa tgtatatgct ttccatgatt
     2641 ctgttgaaga tggtaatgga ttagtatatc agcttaataa tatgaagtat gatttaatgg
     2701 cagatgctaa tgatacaaca ttttcacgaa ctaatgatgg aggaaatgta atttataagt
     2761 tagcgggtat taaggatttt tcaattagag gatatgtggc agatagtaat gataaattgt
     2821 ttgaacaaat taaaatttac gcttccaaag atggaaatac ttgggaaagt atagaaatca
     2881 aagtagacgg tataaatgct ttaagcaatc catattggca ttatgcaaat attattcctc
     2941 aagaagcaat tactaaggat tatgattatt taaagattgc tattgaagga acattaacag
     3001 atcattggaa gattcaagtt ggcgatgtac gtattcaaga caaaccattt aattctgttg
     3061 ttaataaaat tgaattagca aatcaagatt tgaaattagc agttggaaat agccaaataa
     3121 ttggggctaa ggtttatcca gaagcagtat tcccatttaa agttattttt gatgctgctg
     3181 ataatgagat tgtttctgtt gataaagatg gtttagtaac tgggcttaaa gctggtaaaa
     3241 caacagttcg tgtttattct caggataatc cagaaattta tcaagactgt acgattgaag
     3301 ttacagaagt gattgacagt tcaaaggaaa ttgcagatag tattaaggag ttaacgatta
     3361 ttaataatca aatcgtttta ccggttgttg aaggttatga aattacaatt aaaagctcaa
     3421 gtatccctaa tatcatcgat ttagatggta aagtaacacc aagtaatcag gatgaacaag
     3481 tagcattggt cttagcagta aagaataagg ctacgaatgt agtatctgat actgcaaata
     3541 ttattgtaac tgttgctaaa aaagatgaaa cagttatgat tgaatttgat attactgatg
     3601 ttgttttaaa agtttatcaa gaatttgatc cactagcaga tattaaagta tttgaaaatg
     3661 atacagatat tacaaagaat gtacggatca taagtaataa tgttgattta aaccatcatg
     3721 gtaaatattt tgttacatat gaagtaactg atagtaaaaa tattacgcat agccatactc
     3781 gcaatgttga ggttagagga attaaatcaa tcagttgtcc agagtatcaa gttgatctta
     3841 ctagtttgaa ggacctggac ccagatgtat ttttaaatat tgttaaagta gataaacaag
     3901 ttattttaga taagctcaat agcaaattaa ttgatgacca aataattatt agctgttatg
     3961 atttggcaat gatgttaaat aacaaagaat atgtactgac cgataatcaa ttaacgattg
     4021 tatggaatgt tacatcaaag atgcagggat tcaaagttct gatgctagat aaggatggaa
     4081 atgtagttga ggttccaatg actgttaatg gtaaggaaat tacttttatg gttgaacagt
     4141 taggtacttt tgcaatagtt ggtgaacaaa aagtagaaac aaaagaacct gatgaaaata
     4201 agccagaaaa taaggttgaa acagcagtta aaactggtga tgaagtttat tatgcggaat
     4261 atgctgtgtt aggattattg gcactatcat tagtttatgg aatgaaaaaa agaggataaa
     4321 acaaaaccca agcttattga aaaaaagtaa gcttgggtta ttttatttgt ttaatatgtc
     4381 gatggtcgat aatatgatcg gtttcaagtt ttaattctag attttcatca tagtgtcgag
     4441 caaataggca gctatagata atatcaagca tgatatggat ggaaatcgtt gaaccaaaag
     4501 tagaaacttt tgaataaatt cgttctcgag aatctaagaa taaaacatag tcacatagtt
     4561 ttgaaaggcg gctttctcca aatgaagtaa ttgcaatcat cggaacctta cggcgtttca
     4621 atagttctgc ggcagcgatt acttcgttag tttcaccaga atatgaaatt aaaatggcac
     4681 aatgatcctt atttgattgg gcagcttgaa atagttgttc tttgaccatt ggtggaatct
     4741 gaacattttt tccgattcga aacatcttat attgaaaatc agaggcaatt ctaagtggac
     4801 ctgagacacc ataaatatca ataactttag tttgatcgag cataataatg atttttgcta
     4861 agatatcatt gtcaatatta tttacagtaa ggttgattga atctttagct agtgttgcaa
     4921 tgctgtgttt gattgtatgg atcgtatctc tttttttaaa aggaaaattc ggatttgttt
     4981 cactatttct atcaaacgta tattgtaact gagctgataa tgcaatttta aaatcattat
     5041 atcctttata ctctaatttt tgacatagtc tagttattgt agcgggcgaa gtatacgttg
     5101 ctttagctaa ctgagaagta gacatattta aaacatcggg tccatttttt aaaatatatg
     5161 ttgcgattaa tttttcggaa gttgagaagt caacggccat ttctagtttt cttagtagac
     5221 tcatttttat caccaatttt agtataccat ttttttatta ttaagtttaa ttatttagtt
     5281 agataaatca aaaaattgct ttttttcata aatatgaaat gcttttttaa tcaaaattga
     5341 atttaataat cacaattttt aatatttaat tttttggata agcgaagaat tatatattgc
     5401 aaaacttctt gtttcgtgtt tatgaaatat catttcataa attgcccaat aaaataataa
     5461 aagtttgaaa ggagattgca actatttgtg taagtgttta caatcgttgt ttagacaatt
     5521 aaactaaaag tataaatgga gattagtaag gaggagaaaa atttaattaa tttatgaggg
     5581 aaaaatataa aggagaaagg aatattatga ataggaaatt taagcgattt ttgacttcat
     5641 tattatcatt agcggtagtt gctagttctg gaattggacc agtaaaagcc gttgataata
     5701 cccctaaact ggattcaaaa gtttatatta acagtgataa tgtattaact aaagattttg
     5761 aaggatttgg ggtacaatgg gatcctagtg atttatttga ttacactgat gaacaatggt
     5821 catcttttta tgaaaaagca agctttttaa gccctaatgt gatgcgggtc atgctccatg
     5881 atggggacag ttattgtatt ggctttgaag acgatggaac tccgatttat gattgggagt
     5941 ctgttatgat gaagcgtgtt tataaaattt tagattttgc tcaacaaaat gatattccaa
     6001 tcatgttagg tgaatggaga agtattagtg aaagaggata tttatcttac gatgatcatg
     6061 gaaaaacggt aaattggagt agtcctacat gggcaaggat gattggtgat tgtttagagc
     6121 atttgattgt tgataaaggt tatacttgta ttaaatacta taacatgatt aatgaaccaa
     6181 attattataa acgagatcat ggtgatgtca caaatgaata tgtatatgat caatggaagc
     6241 aggcaattac taatttaaga aatgaaatgg acagttctgg tatagaaaaa attgaaaata
     6301 tcaaaattgt agggccggat gtttatgatt cacaagaagc ttggataaat caagcaacaa
     6361 gtgatgagat gaaagataaa atagaattaa cagaagtgca tagatatgcc ccacaaagtg
     6421 aggtagaaag tggacttatt gaaaagaaat taaagacttg gaaagaacag gctgaaagtc
     6481 ttgatcctga agttgctgag gaaggatttg ctttgggtga aatgggaatt tcaggaactg
     6541 gtcctggaga ttcgcaattg aatgcgagaa aatacgatta tggagtggat atttttgatt
     6601 atggtgtcca agctattcgt gcgggattaa aatttggtag cgtatggggc tttgaagata
     6661 gtatgcatgt tcagcataat gatattgtca ataatttcaa agatcaatat ggacctgctg
     6721 caacaacaga agaaggtaga gattatgttg tccatacgcc aacaggagat ccttcaattg
     6781 ataatgatat caagatatgg ggattttgga atgaacttgg tgaagaaatg gcagcccaaa
     6841 atgctgagca taatgttacg ggaagagcta atacagtaaa agcaagtgat gagaatctaa
     6901 aaccatggta ttatacttgg tcaatgttat gccgttattt tccatctgga acaaagattc
     6961 ttgaaactac agacagttat gttgatcagt taagagttac atcaggaatt attccagttg
     7021 cagatgataa aggtgatatt agtattgcag ttgttaataa ttcatcatct caaaaaacat
     7081 tagaagtaaa tatgcctaat gcagctagta aagttgatct taatcaatat ttctattatg
     7141 atggtgaaat tgatgggaaa actagaccgg tcaatgaaaa gggtcaattg ttacaatatg
     7201 acacaattga aaatgctaat cttaaggatg gtgttaatgt cactttacca gcaaaatcat
     7261 gtatgatctt aacttcttta ggatatgagg gtgaaagtca tccaatgagt tttacaactg
     7321 gtcaaacaac tgatgttgaa aaggttgaaa tttatgaaac tactaatgct aaccaattag
     7381 aagttggtaa gtcttatcaa ttagcagcta attatattcc atctatttca aaaggtgaaa
     7441 tggaatggtc agttgctgat tattttggta atgctagtga taaagcatta attgatgaaa
     7501 atggactttt aacagtaaaa aaagctggtc aatttaaagt gattggtaat ttaaaaggaa
     7561 aaccagaaat acaggataca ttagtattta aggcaacaag ttctagtatt ttagttgatg
     7621 agttaaatga tcttgaaaat ggagttgctt tatcttatca agatattata aaagatgata
     7681 attcagctaa ttttaatggt gataaaacag ttaaaagaag tgatagtaat gcaaatggta
     7741 aaccaggaat tattacatat caagcaaata acatttatga ttttgaattt agtgcatatt
     7801 cattaaataa taatcttgat aaatctggaa attttgtagt agaagtttct caaaatggtg
     7861 atagctggtc accagttgaa tgtgagttta ttcaaggaag taaattaagt tctggctggt
     7921 atccatatac aattaaaaat aaagcggtaa taaaagatga tggatatcaa tatttacggg
     7981 taacgatcac aagtaagtca ggttataaaa catatgatcc tcaatatgct ggtggatcaa
     8041 tatattatga ttaccaggga gcatcacaaa tcgatattca aagtcataat gaatttattg
     8101 ttaaaggaca agaattacag tttaaagcag aagtacttcc aagtattgcc agccaagaag
     8161 taagctggaa agtattatct cttgagggaa aaccaactga acttgcaaca ataagtccag
     8221 atgggatatt gactgctaag gctaaaggag aagttgtcgt tgtagcaact gctaaagata
     8281 ctgatgttag tgcttatagt catattaata ttattaatgg ttattttgta gatgaaattg
     8341 aagattttag taaaatgtat caatatggtg aatttgcttt tgatgatcct aaatcatcaa
     8401 atttcactga taaaacaagg attaaaagat tatcagatag taatcaaagt attgtttatg
     8461 ctttaagtga tattgaaaaa gctacttttg aaatctataa aaatggttca tttgtaaatg
     8521 attcagtaga tatttatgct tctagtgacg gattaactta taataaagta gagaaaaatg
     8581 ttgtaaatgc aggtaaagca gctagttcaa ctgaatatta tttatatgaa gtatcgacta
     8641 aagagttagg tgataatgta aattatatta agtt
//
LOCUS       DAOMTA010000019         8639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_79_length_8639_cov_2.173928, whole genome shotgun sequence.
ACCESSION   DAOMTA010000019 DAOMTA010000000
VERSION     DAOMTA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_8639_cov_2.173928"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1698
                     /gene="spoIVA"
                     /locus_tag="PLK68_01100"
     CDS             223..1698
                     /gene="spoIVA"
                     /locus_tag="PLK68_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HRM89037.1"
                     /translation="METKNILHDIAKRCDGDIYLGVVGPVRSGKSSFIKRFMEMAVIP
                     YIEDKDAKLRAIDELPQSGKGKMIMTVEPKFIPNQAVEMLMDENFKVNVRLVDCVGYV
                     IEGAKGYQDDQGIRYVKTPWYLESIPFDQAAKVGTKKVIQDHSTIGIVITSDGSICDI
                     PGANYNEATDSIVEELLDIDKPFIIIINTKDVNSPACKKEYERLTAKYDVPVLSMDVT
                     NMEEPQIVSLLKDALYEFKISEVRIEVPKWLAMMSNSHWLKQTLDTSLKESLQSIKKF
                     KDVERIGDTISEYDFVDKAYLTGIDTTTSSATLKIEEREGLYNEILEEIIGDKGFDQA
                     TFLTFIQELVDIKKEYEGFSNAIRMVKQTGYGYAIPKLDEIELSDPEIIKQGPRYGMK
                     LVSKAGTTYMIKVDIESTFEPIIGSREQAEAFIEYLNSSGNDKQAIFDCDVFGRKLGD
                     LIEEGMYIKLNAIPENASLRLHDILSKIVNKGKSNVIAIVL"
     gene            1763..2035
                     /locus_tag="PLK68_01105"
     CDS             1763..2035
                     /locus_tag="PLK68_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM89038.1"
                     /translation="MNKKELVETVSKERNLTKKDAEILVDTVFNTITRSVVEGDKVLI
                     SGFGTFKVNDRKARKGISPKTQEEMVIPASKTVTFKPSNRLKDAMN"
     gene            2109..2816
                     /locus_tag="PLK68_01110"
     CDS             2109..2816
                     /locus_tag="PLK68_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRM89039.1"
                     /translation="MPFYYFPMNSYYMFGYLLVIIGSLIMIYGQIKVNSAYKRYERIP
                     NSRGITGAVVAREILDRNGLSDIQIHVVNGKLSDHYNPRNKTINLSREIHDGTSIAAL
                     AVASHECGHAIQHLVGYKPLVFRNAILPLCNVGQYLGWIAVFIGLIMGNTSVAWIGVF
                     LMGGILLFQIVTLPVEFDASSRALRILKANYLTTDEYSGAKSMLSAAAFTYVAAMLST
                     VLSLLRIVLIVIGNDRD"
     assembly_gap    2910..2919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2975..3463
                     /locus_tag="PLK68_01115"
     CDS             2975..3463
                     /locus_tag="PLK68_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89040.1"
                     /translation="MKFLRKNRFNILIVFLVLVLIVVSFYMLWVHVPYSKHKNEITSI
                     ENVIVKENNYSNVKYFDRYNSDKSYYIIRATKGKKQQYAVFDEKYKLIKSYSGDVVDQ
                     QVCINAFKEKYKQEPSEVSVGYENEIFVYCLMYQGKDSLVYAFYGIDNGEFVKAYRID
                     NG"
     gene            3468..4067
                     /locus_tag="PLK68_01120"
     CDS             3468..4067
                     /locus_tag="PLK68_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRM89041.1"
                     /translation="MLKSLVESKCINIERLLVLSAKELKIDGNECHILLLIYTLMEAG
                     IKTVTPQMIQNYSMLTSHDLNKVLQSLLNKKLIYNRAGSISLNHLEDKLLQDHESASA
                     KQEEESLNIVTIFEEQFGRPLSPIELNIIKDWKESDYSDEMIVKALKEAVKSQVLNFR
                     YVEGILNNWAKNGIKQRYVESEQPQRKVPISEYKWWENE"
     gene            4060..4701
                     /gene="nth"
                     /locus_tag="PLK68_01125"
     CDS             4060..4701
                     /gene="nth"
                     /locus_tag="PLK68_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRM89042.1"
                     /translation="MNKEKTNRVLEYFDELFPDAYCELNHESDFQLLVAVMLSAQTTD
                     KKVNQLTENLFKKYPTVEAVSQASLPELEQDIKTIGLYRNKAKNLLALSHVLIEQFDG
                     IVPSDQKQLESLPGVGRKTANVVRSVAFDIPAFAVDTHVERISKRLGFAKRDDNVLTV
                     EKKLCRSIPRNRWNKSHHQFIFFGRYFCKATNPSCTECKLFDMCKDPIKNKYL"
     gene            complement(4764..>5327)
                     /locus_tag="PLK68_01130"
     CDS             complement(4764..>5327)
                     /locus_tag="PLK68_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HRM89043.1"
                     /translation="EASINGNGKIDVSFTAYEPANPDIVYVVEVYDESNNLLYSTKLT
                     TNSGTIDYAPTGKVKVVGYYAYSSGSPTSNKIEKTIGSSAKLDKVNYSITSGGSSVTN
                     GSTTATSSVAIKVNAQSSSNTITIQFMNSSGGTIGSPSTFTGSATKEFALTPGAQYTV
                     KITESDGTNTVSASISFTVGGNSNPGE"
     assembly_gap    5328..5348
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5349..7439)
                     /locus_tag="PLK68_01135"
     CDS             complement(<5349..7439)
                     /locus_tag="PLK68_01135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRM89044.1"
                     /translation="MTDSPTRKRKTKKKLNWKKVATSLVVVILIVCLGMGCFFAWSVY
                     KETDDFSAKKLLSGEASKMYDINGELMYTFGSDTNGKRENVTYEDLPQVLIDAVVAAE
                     DSRFFEHNGFDLPRIAKTMITNLAHLSIRGGGSTITQQVIKKSYFPKEEQTYTRKLSE
                     IILAIQATKEISKEEIITLYLNKIYFGRSTSSIGIAAASKYYFNKDVSQLTLPEAALL
                     AGTLNSPSAYDPYYNLEKATQRRATVLNLMVDHGYITQEECDLAKQVKVENTLSQGIT
                     SSDDALAAYVDIVTKEVKEKTGLDPAETQMNIYTYCNQEVQKMATAMANGETYKYSDE
                     DMQMGGSVQSTQDGRIIAVIGGHNYKSGDLNKATVKQQPGSSMKPLLDYGLAYKYLNW
                     STVNTVKDEPLTLGGKTIKNWDGQYHGSMSISDALLNSWNIPAITTFQEVKKAAGSDA
                     IIKDMESVGIDMSDQDKELSDTYAIGGWKTGVSPVELAGATIANNGNYIESHTINYVE
                     VVETNKTVKIDEELQKNVTQSIGEDVSFMIRETMLSYSSSTTGSYSSLSGLDNVGAKT
                     GTSNWDSTSPYVKAGKSRDLWMTAYTPDYTCSVWMGFDTTGIKKGKNTSDYKAYPAKV
                     VAELLKYLQKNTTDKKSYPEQPSGVEQIQVVAGTYDSPTANTPASQILTGWAKTGYGP
                     TTAAKDPEINTLSAF"
     gene            complement(7429..8028)
                     /gene="recU"
                     /locus_tag="PLK68_01140"
     CDS             complement(7429..8028)
                     /gene="recU"
                     /locus_tag="PLK68_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HRM89045.1"
                     /translation="MVNYPNMKKTTVHQTKLIDGKLNTRHRGMNLEEDLNLTNKYYLA
                     RKIANIHKKPTPIQIVKVDYPKRSAARIVEAYFKTPSTTDYNGIYKGKYLDFEAKETK
                     KQNFPFTNISVHQIEHLKSVIEHQGIAFVIIAFTHLNEVYLVNASYVIDAYYQPDQKS
                     ISYQTVKEKGHLIEQGFNPRLDYLKIIDQYYLGGHENDR"
     gene            8220..8555
                     /locus_tag="PLK68_01145"
     CDS             8220..8555
                     /locus_tag="PLK68_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRM89046.1"
                     /translation="MEKKVMLSPKKIIAKEFKVDFKGYNAEEVDHFLDMVVNDYEAFA
                     AMLNASYDKIDQLEARLSEQKIKIAKLEREKALQDDNIHAMEENLSTNVDILKRLSLL
                     EKVVFNQNR"
BASE COUNT         2914 a         1220 c         1547 g         2927 t
ORIGIN      
        1 atgaatagat aatttggacg aacagctgga ccatatgaat aattaggttt acattcttgc
       61 atcccgcaaa atttcaagta aaaatcttca aaagatttat tctctaattc taaatatgag
      121 tacatggaaa tacctccttt atcaattata ccaaattaat aattagatgt gttctaaata
      181 taggcatatt tcatataaat aatacgaaga gaggagttta caatggaaac gaaaaatatc
      241 ttacatgata tcgcaaaaag gtgcgatggg gacatatatt taggggtggt tggaccggtt
      301 cgaagtggga aatcttcatt tatcaaacgt tttatggaaa tggcggtcat tccatatatc
      361 gaagataaag atgcaaaact gcgtgccatt gacgaattgc cacagtctgg taaaggtaaa
      421 atgattatga ctgtagaacc taaatttatt cctaatcaag cagtagaaat gttaatggat
      481 gagaatttta aagttaatgt ccgactagtt gattgtgttg gttatgttat tgaaggagca
      541 aaaggatatc aagatgatca aggaatacgc tatgttaaaa cgccttggta tttagaatca
      601 attccatttg atcaagcagc caaagtcgga acgaaaaaag ttatacaaga tcattcaaca
      661 attggaatcg ttataactag tgatggtagt atttgtgata ttccaggagc taattataat
      721 gaagcgactg atagtattgt tgaagaacta ttagacattg ataagccgtt tattattata
      781 attaatacta aagatgtcaa cagtcctgct tgtaaaaaag agtatgaacg tttaaccgct
      841 aaatatgatg ttccagttct atctatggat gttacaaata tggaagaacc acaaattgtt
      901 tcattattaa aagatgcatt atatgaattt aaaatcagtg aagtacgtat cgaggtacct
      961 aaatggttag caatgatgtc aaatagtcat tggttaaaac aaacattaga tacatcatta
     1021 aaagaatcat tacaatcaat taaaaagttt aaagatgtag aacggattgg tgatacgatc
     1081 agcgaatatg attttgttga taaagcttac ttaacaggaa tagatacaac tacttcaagt
     1141 gctactttaa aaattgaaga acgtgaaggg ctatataatg aaatattaga agaaataatt
     1201 ggcgataaag gatttgatca ggcaactttc ttaaccttta ttcaagaact agtagatata
     1261 aaaaaagaat atgaaggttt ttcaaatgct attagaatgg taaagcagac aggatatggt
     1321 tatgcaattc caaagttaga tgaaattgag ttgagtgatc cagaaattat taaacaagga
     1381 cctcgttatg ggatgaaact tgtttctaaa gcaggaacta cttatatgat caaagttgat
     1441 attgaaagta cttttgaacc aattattgga tcgagggaac aagcagaagc atttattgaa
     1501 tatttgaatt ctagtggaaa tgataaacaa gcgatttttg attgtgatgt atttggtcgt
     1561 aaacttggtg atttgattga agaaggtatg tatattaagc taaatgcaat ccctgaaaat
     1621 gccagtttaa gactgcatga tattttgtca aaaattgtta ataagggcaa aagtaatgta
     1681 attgcaattg tgctataaaa gtgcttgaaa tcggtacttt ttaatgttat actttcgaag
     1741 tattagaatt atggaggttg ttatgaacaa gaaagagtta gtcgaaacag tatctaaaga
     1801 aagaaactta actaaaaaag atgccgaaat tttagtagac acagttttta atacaattac
     1861 gcgtagtgtt gttgaaggag ataaagtttt aatctctgga tttggaacat ttaaagtaaa
     1921 tgatcgtaaa gctcgcaaag gaatttcacc aaaaactcaa gaagaaatgg tcattcctgc
     1981 aagcaagaca gttacattca aacctagtaa tcgtttgaaa gatgcaatga attaatcatt
     2041 gcatttttat tttattaata ataagttaat aatatagtgt ataatagagt gtaaaggagt
     2101 tgatatatat gccattttat tattttccaa tgaatagcta ttatatgttt gggtatttat
     2161 tagttattat tggatcatta attatgatat atggtcaaat aaaagttaat agtgcatata
     2221 aacgctatga aagaatcccc aatagccgag gtataaccgg agcggtggtt gcacgagaaa
     2281 ttttagatcg aaatggattg agtgatattc aaattcatgt tgttaatgga aaattatctg
     2341 atcattacaa ccctcgaaat aagacaatca atttgtcacg tgaaattcat gatggaacat
     2401 cgattgcggc acttgcggta gctagtcatg aatgtgggca tgcaatccag catttagttg
     2461 gttataagcc gctcgtattt agaaatgcta tcttgccatt atgcaatgtt ggacaatatt
     2521 tgggatggat tgctgtattt attggtttga tcatgggaaa tacaagtgtt gcttggatcg
     2581 gagtattttt aatgggagga atcttgttat ttcaaattgt tactttgcca gtagaatttg
     2641 atgcttcttc aagagcatta agaatcttaa aagctaacta tctcactacc gatgaatata
     2701 gtggggctaa atcaatgtta tcagcagcgg cgtttactta tgtggcagcg atgttgtcga
     2761 cggtactgtc attattaaga attgtattga tcgttatagg taatgatcgt gattaaaaat
     2821 ctcttttaga gattttttct ttctggtacg aagtaataaa cgcagcgaat actattatcg
     2881 aaagatctga aaatcccgac gtgaactggn nnnnnnnnnt ttatattatg ataattgata
     2941 gtataataat taagcgagga aagttggtgt tattttgaaa tttttacgaa aaaatcgttt
     3001 taatatatta attgtttttt tagttttagt tttaatagtt gttagttttt atatgctttg
     3061 ggttcatgtt ccctattcaa aacataaaaa tgagattaca agtattgaaa atgttattgt
     3121 taaagagaat aattatagta atgttaagta ttttgaccgc tataatagtg ataaaagtta
     3181 ctatattatt cgtgcaacca agggcaaaaa acaacaatat gcagtttttg atgaaaaata
     3241 taaattaatt aaatcttata gtggtgatgt agtggatcaa caagtatgta ttaacgcatt
     3301 taaagaaaaa tataaacagg agccaagtga agtttcagtt ggttatgaaa atgaaatctt
     3361 tgtctattgt ttaatgtatc aaggaaagga ttcactggtt tatgcttttt atggaattga
     3421 taatggggaa ttcgtaaagg catatcgaat tgataatgga tagaatgatg ttaaagagtt
     3481 tagttgaaag taaatgcatt aatattgaac gactactggt gttaagcgca aaggaattaa
     3541 agattgacgg taatgaatgt catattttgc tgcttattta tactttaatg gaagcaggga
     3601 tcaagacagt aacgccacag atgattcaaa attattcgat gttgacaagt catgatctta
     3661 ataaggtgct acaaagttta ttaaataaaa aacttattta taaccgagct ggttcaatta
     3721 gtttgaatca tcttgaagat aaactattac aagatcatga atcagcgagt gctaaacaag
     3781 aggaggagag tcttaatatt gttactattt ttgaagaaca atttggacga cctttatcac
     3841 caattgaatt aaatattatt aaagattgga aagaaagcga ttatagtgat gaaatgattg
     3901 ttaaagcctt aaaagaggca gtgaagagcc aagttcttaa ttttagatat gtagaaggga
     3961 tattgaataa ttgggctaaa aatggtatta aacaacgcta tgttgaaagt gaacaaccac
     4021 agcgaaaagt tccaattagt gaatataaat ggtgggaaaa tgaataaaga aaaaactaac
     4081 cgagtattag aatattttga tgaattgttt ccagatgctt attgtgaact taatcatgaa
     4141 agtgattttc aattattggt tgcagtaatg ttatcagcac aaacaacaga taaaaaagtt
     4201 aatcagttga cagaaaattt attcaaaaaa tatccgactg ttgaagcagt aagtcaagct
     4261 agtttaccgg aattagagca ggatattaaa accattggac tttatcgaaa caaagctaaa
     4321 aacttactag cattatcaca tgttttaatt gagcagtttg atggtattgt accaagtgat
     4381 caaaaacaat tagagagtct accgggggta ggtcgtaaaa cagcaaatgt tgtgcgttct
     4441 gtagcatttg atataccagc ctttgcagtt gatacgcatg ttgaaaggat ttctaaacga
     4501 ttaggctttg ctaaaagaga tgacaatgtt ttaactgttg aaaagaaact atgtcgttct
     4561 ataccaagaa atagatggaa taaatcccat catcaattta ttttctttgg aagatacttt
     4621 tgtaaagcaa ctaaccctag ttgtacagaa tgtaaactgt ttgatatgtg taaggatccg
     4681 attaaaaata aatacttata ggcgcctgag gatgtctttt taataaaaaa tatcagcttt
     4741 attaaaagct gatattttta ttattattct ccagggtttg aattaccacc aacagtaaaa
     4801 ctgattgaag cactaactgt attagtacca tcactttcag tgattttaac ggtatattga
     4861 gcacctggtg ttaatgcgaa ttctttagtt gcactacctg taaaggtact aggtgaacca
     4921 attgttcctc ctgaagaatt cataaattga atcgtaatcg tattactact gctttgagcg
     4981 tttactttaa ttgcaactga actggttgct gtggtcgaac cattagtaac agaagaacca
     5041 ccggaagtaa ttgaataatt tactttatct aatttagcac ttgaaccaat tgttttttcg
     5101 attttattag atgttggact accgcttgaa tatgcataat atccaacaac ctttacttta
     5161 ccggttgggg cataatcaat cgtaccacta tttgtagtca atttagtact atataaaaga
     5221 ttatttgatt catcatagac ttcaacaaca tagacaatgt caggatttgc aggttcatag
     5281 gcggtaaagc taacatctat tttaccatta ccattaatag aagcctcnnn nnnnnnnnnn
     5341 nnnnnnnnga atgctgataa agtattaatt tcagggtctt tagctgctgt agttggacca
     5401 tatcctgttt tagcccatcc tgttaagatt tgactagcag gtgtattagc agtcggacta
     5461 tcatatgttc ccgcaacaac ttgaatttgt tcaacaccag atggttgctc aggatatgat
     5521 tttttgtcgg ttgtattttt ttgtaagtat tttagtaatt cagcgactac tttagctgga
     5581 taagctttat agtcagaagt attcttacct tttttgatac ctgttgtatc aaatcccatc
     5641 catactgaac atgtatagtc aggggtgtaa gcagtcatcc ataaatctcg gctcttgcct
     5701 gctttaacat atggactagt actgtcccag ttagacgttc ctgtcttagc gccaacgtta
     5761 tcaagtccgc ttagtgatga ataacttcct gtagtactgc ttgaatagct taacattgtt
     5821 tcacgaatca tgaaagatac gtcttcacca atgctttgag taacattttt ttgcaattct
     5881 tcatcaattt tgacggtttt atttgtttcg acaacttcaa catagttgat tgtatgagat
     5941 tcgatataat taccattatt tgcaatcgtt gcaccagcta attcaactgg acttacaccg
     6001 gttttccagc caccgattgc ataagtatca cttaattctt tatcttggtc agacatatcg
     6061 ataccaacag attccatgtc tttaataatc gcatcgctgc cagctgcttt ttttacttct
     6121 tggaaagttg taatggctgg gatattccat gaattaagca gggcatcaga aatactcatt
     6181 gaaccatggt actgaccatc ccagttttta attgttttac cgcctagagt caatggctcg
     6241 tctttaacag tattaacagt actccaattt agatatttgt aggcaagacc ataatctaat
     6301 agtggtttca ttgatgaacc aggctgttgt tttacggtag ctttatttaa atcaccggat
     6361 ttgtagttat gtccaccgat aacggcgatg atccgaccat cttgagtaga ttgaacacta
     6421 ccacccattt gcatatcttc atcgctatat ttataagttt caccattagc cattgctgta
     6481 gccatttttt gtacttcttg attgcaatat gtatagatat tcatttgagt ttctgcaggg
     6541 tccaagccag ttttttcttt aacttcttta gtgacgatat ctacataagc agctaaagca
     6601 tcgtcagaag aagtaatacc ttgtgataaa gtattttcga cttttacttg ttttgctaaa
     6661 tcacattctt cctgagtgat gtacccatga tcaaccatta aatttaaaac tgttgctcga
     6721 cgttgagtcg ctttttctaa attataatat ggatcatatg cacttggcga attcaatgtt
     6781 cctgctaata gagcggcttc tggaagcgta agttgtgaga catctttgtt aaaatagtat
     6841 ttactagctg cagcaattcc gattgagcta gtgctgcggc caaaataaat tttatttaaa
     6901 tataaagtaa ttatttcttc tttactaatt tctttagttg cctgaattgc aagaatgatt
     6961 tcacttagtt tacgcgtata agtttgttct tcttttggaa agtaagattt tttaattacc
     7021 tgttgagtaa ttgttgatcc tcccccacga atacttaaat gagctaagtt agtgatcatt
     7081 gttttagcaa tacgtggtaa atcaaaaccg ttatgttcaa agaaacgaga atcctctgcg
     7141 gcaacaacgg catcaatcaa tacttgtggt aaatcctcat aagtaacgtt ttcacgttta
     7201 ccatttgtgt cactaccaaa tgtatacatt aattcaccat taatatcata catcttactc
     7261 gcttccccag aaagtaattt tttagctgag aaatcatcgg tttctttgta tactgaccag
     7321 gcaaagaagc aacccatgcc taaacagact attaaaatta caactactag agatgtagcg
     7381 acttttttcc aatttaattt tttctttgtt tttctttttc ttgtagggct atctgtcatt
     7441 ttcgtgacct cctaaataat actgatcaat tatctttaaa taatctaagc ggggattaaa
     7501 tccctgttca attaaatgtc ccttttcttt aaccgtttga taagagatag atttttgatc
     7561 aggttgatag tatgcatcaa ttacatatga tgcattaact aagtatacct catttagatg
     7621 ggtaaaagca ataattacaa aagcaattcc ctggtgttca atcacgcttt ttaaatgttc
     7681 gatttgatga acactaatat tcgtaaaagg aaaattctgt tttttagttt cttttgcttc
     7741 aaaatccaga tatttacctt tatatatccc attatagtcg gtggttgaag gagtcttaaa
     7801 atatgcttca acaattctag ctgcagacct tttaggataa tctactttaa caatctgaat
     7861 cggggtaggt tttttatgaa tatttgcgat ttttctagct aaataatatt tattagtcaa
     7921 atttaaatct tcttctaaat tcattccccg atgacgagta tttaactttc catcaattag
     7981 ctttgtttga tgaacagttg tttttttcat atttggataa ttaaccatgc ttgctccttt
     8041 ttattacttt acgaaggtat tataccatat ctgtcttaaa atatcatagt atcttatgag
     8101 tttccaaaac attgtcaatt taagtaaaat cactaacatt atgttaataa tcgattaatc
     8161 gcttgcattt aagacaattt tttgtttcaa tataaagtag agataaggag ttgataaact
     8221 tggaaaagaa agtaatgtta agccctaaaa agataatagc taaagaattc aaagtagatt
     8281 ttaaaggata taatgctgaa gaagtagatc attttttaga tatggttgta aatgattatg
     8341 aagcttttgc agctatgtta aatgcttcgt atgataagat tgatcaactg gaagcgcgtt
     8401 taagcgaaca aaagatcaag attgctaaac ttgaaagaga aaaggctctt caagatgata
     8461 atatccatgc gatggaagaa aatctttcta caaacgttga tattttaaaa agattatcgt
     8521 tattagaaaa agtcgtattt aatcagaata gataattgtt aataagacac tttataagca
     8581 ataaagtgtc ttttattatg atgtaaaagg gttttgacag taaaaatatc gatgtcaaa
//
LOCUS       DAOMTA010000020         8543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_83_length_8543_cov_2.672008, whole genome shotgun sequence.
ACCESSION   DAOMTA010000020 DAOMTA010000000
VERSION     DAOMTA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_8543_cov_2.672008"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLK68_01150"
     CDS             complement(<1..705)
                     /locus_tag="PLK68_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM89047.1"
                     /translation="MHKLACPKCHESIVQEGNSYKCANNHCYDLAKTKYLNLLLNPDK
                     ATNNPGDSKESLVARKAYLTKGYYDVILNSVIDCIKKYRDDTPLDILDLGCGEGYYTR
                     GLKEIFSQDTIYGLDISKEAINMATKYTKDVYWLVGNSKNLPIVDHSLDFITALFTVV
                     NQDELKRTLKKGGYIIHVTANPNHLIEIKELIYDEIKVKSDKYIRLDFETIESYDLVH
                     QVKIDNREDALNLLKMT"
     gene            778..>1656
                     /locus_tag="PLK68_01155"
     CDS             778..>1656
                     /locus_tag="PLK68_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM89048.1"
                     /translation="MILTVMMVVIAILINIFIAYHFKKYLNKHRVVAAVIIVLVAVSV
                     SIEMIILGFLINLCICLIIADLIYLLIFKTKLKRPYQKVYLIGVLICSLSLSVYGIYN
                     ANNIVITNYQVSINKEFKDKKIMALSDIHLGTAVKTVDLKQLVTQAEKIKPDMIFLVG
                     DVYDENTSSDEIDDSMKIFTQLAKSYPVYYVIGNHEVGYSSSPLKEYNILERLQLAGV
                     NTLNDEYVEFEDINIVGRQDYKIKKRKPVEQIINGMNQNKPVILLDHQPRSLEENKKL
                     GIDLMISGHTHAGQVFP"
     assembly_gap    1657..1717
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2000..3064
                     /locus_tag="PLK68_01160"
     CDS             2000..3064
                     /locus_tag="PLK68_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM89049.1"
                     /translation="MSWFVLMEMTLVAVMTVYLAYHTHLVFAKAKYRKIITTVIIGIL
                     IGLCLYNLIFFGFLANACICFVIFDIINLILYKTRFNRYFKFIYQRGMVALAASVILS
                     FYGIYNAKNTVITTYDVIINKSFVDKSLMVVSDIHLGTVVTKADLTKLSEHAEAIAPS
                     GIILLGDIYDEGTTQDEFDYSLQVFKILASKYPVYYIEGNHEIGFQGGSPLREFNIVK
                     KLKEIGIKVLLDDVTKLDDIYLIGRKDYVVKKREALKDLTEPLDTSKPLILLDHQPHD
                     YELNEQLGIDLQLSGHTHAGQIFPLNFLFSFIRVNDLNYGIEVNNNFHGIVTSGMGGW
                     GYAMRTAKHSEIVVVNLKSS"
     gene            complement(3169..3795)
                     /locus_tag="PLK68_01165"
     CDS             complement(3169..3795)
                     /locus_tag="PLK68_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRM89050.1"
                     /translation="MDKIKDLYQDDLIYIFTRSQLRDLNTDYLAIIKNECSIQTINNC
                     ITMHYQDDFESLLAFSLPTKNRKNKQLISLNQCYKIMDKIHYGVLSFTHEGLPYSVAL
                     NHIIIDNRIFFHCAKSGYKLNSIEQRATYLIVEDLGINLKAGTHNHNSVAVFGTVHEV
                     TEFETKKAALLEIVSHLAPEHPYNDKMVDTTNIIELEIDYINGKTHIR"
     gene            3874..4434
                     /locus_tag="PLK68_01170"
     CDS             3874..4434
                     /locus_tag="PLK68_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRM89051.1"
                     /translation="MTKIRLAVATADDAARLLEIYRPYVLTTAITFEYEVPTLEEFRA
                     RIVSTLEKYPYLVAKLDDKIVGYAYTSAFKSRAAYQWAVETSIYIDLDYKGGGIGSIL
                     YHKLEEITKQQNIINLNACITAGNPESIVFHEHFGYQKVAYFTKCGYKFNQWHDMIWM
                     EKMLGEHPGKPAPVIPFGKLYKHLSI"
     gene            4503..4763
                     /locus_tag="PLK68_01175"
     CDS             4503..4763
                     /locus_tag="PLK68_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89052.1"
                     /translation="MGLFGKKKKTVDLQQVFKDKYKDINQIVNDANNELDLEIQISLL
                     ELAYDKYNDLLDLIDQGVDFDKAHFLSMQQDLKKQIDLLKGL"
     assembly_gap    4840..4849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4850..5576
                     /locus_tag="PLK68_01180"
     CDS             <4850..5576
                     /locus_tag="PLK68_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRM89053.1"
                     /translation="YHKDFDYTYTLGVFETIELLENKPELVMRVYLKSNSYQNKGVKI
                     IENLCQQNKIELIESDNTINKLAKKDNCFAIAIIKKYTMDLEDANHVVLVNPGDMGNM
                     GTIIRTMLGFNYTNLAIIRPGVDVFDPRVIRASMGALFNINFEYFNSFDEYLTKYPER
                     EVYPLMLKGAKNIHQITAANNKHSLVFGNESSGLPDDYLKYGQSVFIPHSDKIDSLNL
                     SMALGITLCHFSMMEFKDKQVLR"
     gene            5604..6788
                     /locus_tag="PLK68_01185"
     CDS             5604..6788
                     /locus_tag="PLK68_01185"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HRM89054.1"
                     /translation="MYDFDKIVKRRNTKCIKWDTWKKEGKPKDILPLWVADMDFETLP
                     EVTEAIIERAKHAVYGYSMAGDDYYEAVCNWMKRRHQLDINADNIVTTTGVVTALKIA
                     VNAFTAPGDAIIINKPVYYPFDFSIDENQRKKIECPMMFTGQTYELDFDLFEQLIIDN
                     DVKMFILCNPYNPIGKVWNKEELFKLGNICKKHNVLVVSDEIHQDFIYKGNKHLPFVN
                     VDASFKEFTIICTAPSKTFNLAGLQTSNILFFNHKLKEKFIKVKSSLGFPVEPTIFGI
                     EACKAAYNHGDKWVDELVAYLDGNIKYLDGFLKKKLPMLKMIKPQGLYLVWVDFSALQ
                     MTHEELEAFMVNEAKLWLDEGYIFGVGGAGFERFNLAMPRCLLVQALDNLYQALKNRQ
                     LI"
     gene            7232..8098
                     /gene="fba"
                     /locus_tag="PLK68_01190"
     CDS             7232..8098
                     /gene="fba"
                     /locus_tag="PLK68_01190"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRM89055.1"
                     /translation="MGLVSATEMLNKAKEGHYAVGQFNINNLEWTKSILLTAEELKAP
                     VILGVSEGAAKYMTGFKTVSAMVSAMVDSLGITVPVALHLDHGSYEGAKAALEAGFSS
                     IMFDGSHYGIEENIEKTKEIVELCHSKGVSVEAEVGSIGGEEDGVVGKGEVADPKECK
                     MIADLGVDFLAAGIGNIHGKYPENWEGLDFDALDAIQKETGKLPLVLHGGTGIPEDMI
                     KKAITLGVSKINVNTECQLYFQEATRKYIEAGKDLEGKGFDPRKLLAPGAAAIQECVK
                     EKMEIFGCIGKA"
     gene            8278..>8543
                     /gene="tnpA"
                     /locus_tag="PLK68_01195"
     CDS             8278..>8543
                     /gene="tnpA"
                     /locus_tag="PLK68_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRM89056.1"
                     /translation="MNKQYSDDLHSLSHTKWSCKYHIVFAPKYRRRAFYEARGVEVGA
                     ILRQLCEWKGVNIIEAEVCIDHVHLLVEIPPKYSVSGFMGFLKG"
BASE COUNT         2956 a         1014 c         1589 g         2913 t
ORIGIN      
        1 ggtcatcttt agtaaattta aagcatcttc acgattatca atttttactt gatgaactag
       61 atcatagctt tcaattgttt caaaatctaa acgaatatat ttgtcacttt taactttgat
      121 ttcatcatat attaattctt ttatttcaat tagatgattt gggttggcag ttacatggat
      181 aatataacca ccctttttta aagtacgttt cagctcatct tgatttacga ctgtaaatag
      241 agctgtgata aagtctaatg agtgatcaac gataggtaag ttcttactat taccgacaag
      301 ccaataaaca tctttagtat atttagtggc catattgata gcttctttag atatatctaa
      361 tccataaata gtgtcttggc taaagatttc ttttaatcct cgagtataat atccttcacc
      421 gcaaccgaga tcaagtatat ctagtggggt atcatcacga tattttttta tacagtcaat
      481 aactgaattt aaaatgacat cataatatcc tttagttaga tatgctttgc gggcaactaa
      541 actttcttta ctatcaccag ggttatttgt agctttatca ggatttaata acaaatttaa
      601 atatttagtt ttagctagat catagcaatg attattggca catttatatg aatttccttc
      661 ttgaacgatt gattcatgac attttggaca cgctaattta tgcataattt cacctcggag
      721 ttattatacc ataaatataa tctgtggtat aatttattct taaaggaggg atagtttttg
      781 attttaaccg taatgatggt agtaattgct atcttgatca atatatttat tgcatatcat
      841 tttaaaaaat atttaaataa gcatagagtt gttgcagcag taattattgt tttagttgct
      901 gtaagtgtaa gtattgaaat gataatttta ggatttttaa tcaatttgtg tatttgtttg
      961 ataattgcgg atttaattta cctgcttata tttaagacaa aattgaaaag accatatcaa
     1021 aaagtttatc ttattggagt acttatttgt agtttaagct tgagtgttta tgggatatat
     1081 aatgcaaata atattgtaat cacaaattac caagtaagta ttaataagga atttaaggat
     1141 aaaaagatca tggcactttc cgatattcac ttgggaacag ctgttaaaac agtagatcta
     1201 aaacagcttg ttacgcaagc tgagaaaatt aaaccagata tgattttttt agttggggat
     1261 gtttatgatg aaaataccag tagcgatgaa attgatgatt cgatgaaaat attcacccag
     1321 ttagctaaat cctatccagt ctattatgta attggaaatc atgaagttgg ttatagtagt
     1381 tccccattaa aagaatataa catattagaa cgattgcaat tggcaggagt caatacttta
     1441 aatgatgaat atgttgaatt tgaggatata aatattgtag gacgtcaaga ttataagatt
     1501 aaaaagcgaa aacctgtaga acagattatt aacgggatga atcagaataa accagtgatc
     1561 ttattagatc atcagcctcg ctcattagag gaaaataaaa agctaggaat tgatttgatg
     1621 atctctggtc atactcatgc tggacaagta tttccgnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc gtcatgatga taattttaat
     1741 gcgatcgttt cttcgggaat ggggacttgg gggtttgcca tgagaactag taaaaattgc
     1801 gagattgtag taattgattt gattagtaaa taagaacata aaaaactaaa cagattttgt
     1861 aatgtattac tatttttgaa gcttaagtgt gcgaaaaaag aagtgatttc aaatgatctt
     1921 agtttagttt ttttattgac aatagtgaga taatttaaga ttattttgtg atataattaa
     1981 ctaaggagat gattttatta tgagctggtt tgttttaatg gagatgactt tggtagcagt
     2041 tatgacagta tatcttgctt atcatacaca tttggttttt gctaaagcaa aatatcgaaa
     2101 gattattaca acagttatta ttggaatttt aatcggcttg tgtttatata atttgatttt
     2161 ctttggtttt ttggctaatg catgtatttg ttttgtaatc tttgatatta ttaatttaat
     2221 tttatataaa actagattta atcgttattt taaatttatt tatcaacggg gaatggttgc
     2281 tttggctgcc agtgttattt taagttttta tggaatttat aatgctaaaa atactgttat
     2341 tactacgtat gatgtaataa tcaataaatc ttttgtagat aaaagcctaa tggtagtttc
     2401 tgatatccat ttaggaacag ttgtcactaa ggcggattta acaaagctta gtgaacatgc
     2461 tgaagctatt gctccaagtg ggattattct acttggtgat atctatgatg aagggaccac
     2521 ccaagatgaa tttgattatt cattacaggt atttaaaata ttggctagta aataccctgt
     2581 ttattatatt gaaggaaatc atgaaattgg tttccaaggt ggctcaccac ttagggaatt
     2641 taatattgtt aagaaattaa aagaaatagg gattaaggta ttgttggacg atgttactaa
     2701 attggatgat atttatttaa ttgggcgtaa agactatgtt gtaaaaaagc gagaagcttt
     2761 aaaagatttg acggaacctt tagatacttc taaaccgctt attttattgg atcatcagcc
     2821 acatgattat gaattaaatg aacaattggg aattgattta caattatcag gtcatactca
     2881 tgctggacaa atatttcctt taaatttctt atttagtttt attagggtta atgatttgaa
     2941 ctatggaatt gaagtaaata ataattttca tgggatcgtg acctcaggaa tgggtggctg
     3001 gggatatgca atgagaactg caaaacacag tgaaatagta gttgtgaatt taaaaagctc
     3061 ctagatataa taagctctgt tgttaaaaca acttaaaggg cattacagct aggaactgtt
     3121 tgatttgaag tattttagta ttacctttgt ttattgtgaa tttgataatt aacgaatatg
     3181 tgtttttccg ttaatataat caatttctaa ttcgatgata ttagttgtat cgaccatctt
     3241 atcattgtat gggtgttctg gcgctaaatg gctgactatt tctaatagtg cagctttttt
     3301 tgtctcaaac tcagttactt catgaactgt tccaaagaca gcgacggaat tatggttatg
     3361 agttcctgct tttaaattaa tacctagatc ttcaactata agataagtgg cacgctgttc
     3421 aatgctgttt agtttatagc cgcttttagc acagtgaaaa aaaatacggt tatcaataat
     3481 gatgtgattt aaagctacac tataaggtag tccttcatgg gtgaatgata aaacaccgta
     3541 atgaatttta tccatgattt tatagcattg atttaaagaa attaactgct tgttttttct
     3601 gtttttagtc ggtagagaaa aagctaataa tgattcgaaa tcatcttgat agtgcattgt
     3661 tatgcagtta ttgattgtct gaatagaaca ttcattttta atgattgcca agtaatcagt
     3721 gtttaagtca cgtaattgtg aacgagtgaa aatgtagatt aggtcatctt gatataaatc
     3781 ttttatttta tccattgcta ttctcctttg ataaagttag tattattata tcatgattat
     3841 acagtttatc ttagaaaaat ggaggaatta tcaatgacga aaattaggct tgctgttgca
     3901 acagctgatg atgctgcaag gttattggaa atatatcgac cttatgtact aacaacagca
     3961 atcacatttg aatatgaagt accaacttta gaagaattta gggcaagaat tgtttcaact
     4021 ttagaaaagt atccgtatct tgttgctaag cttgatgata agatagtagg ctatgcttat
     4081 acatccgcat ttaaaagtcg ggctgcatat cagtgggctg tagaaacatc gatttatatt
     4141 gaccttgatt ataaaggtgg aggaattggc agtatattat accataaatt agaagaaatt
     4201 acgaaacagc aaaatattat taatttaaat gcttgtatta ctgctgggaa tccagaaagt
     4261 attgtgtttc atgaacattt tggttatcaa aaggtggctt attttactaa atgcggctat
     4321 aagtttaatc aatggcatga catgatctgg atggaaaaaa tgttaggaga gcatccaggc
     4381 aaaccagcac cagtgattcc tttcgggaag ttatataaac atctctcaat ttaaaaataa
     4441 caaacttttc aaaactaagt aaacacgcta taattataaa aataacaatg gaggtagttg
     4501 aaatgggatt gtttggtaag aagaaaaaga cagttgattt gcaacaggta tttaaagata
     4561 aatataaaga tattaatcaa attgtaaatg atgctaataa tgagttagat ttagaaattc
     4621 aaatctcgtt attagaatta gcatatgata aatataatga tcttttagat ttaattgatc
     4681 aaggtgttga ttttgataaa gcacattttt tatcaatgca gcaggatttg aaaaagcaaa
     4741 ttgatttatt gaaaggttta taaaatgaaa ataaaaacat atcataaaga ggtatatatg
     4801 caaaggaccg tattggaagg atatatacag aacacatccn nnnnnnnnna tatcataaag
     4861 attttgatta tacatatact cttggggttt ttgaaacgat tgaattatta gagaataaac
     4921 cagaattggt gatgcgtgtt tatctcaaaa gtaactctta tcaaaataag ggtgttaaga
     4981 ttattgagaa tttatgtcaa caaaataaaa ttgaattaat tgaaagtgat aatacaatta
     5041 ataaattagc gaaaaaagat aattgttttg ctattgcaat cattaagaaa tatacgatgg
     5101 atttagaaga tgctaatcat gttgttttag ttaatcccgg agatatggga aatatgggga
     5161 cgattattag aactatgtta ggttttaatt atactaattt agcgattatt cgtcctggag
     5221 tcgatgtttt tgatccacga gtaattaggg catcgatggg ggctttgttt aatattaatt
     5281 ttgaatattt taatagtttt gatgaatatc taactaaata ccctgaacgt gaagtctatc
     5341 cattgatgtt aaaaggagct aaaaatattc atcagattac tgctgcaaat aataaacatt
     5401 cattggtttt tggtaatgag agcagcggac tgccagatga ttatttaaaa tatgggcaaa
     5461 gtgtatttat ccctcatagt gataaaattg actcactaaa tttatccatg gcattaggaa
     5521 tcacgttatg ccacttttca atgatggaat tcaaagataa acaggtattg cgataataag
     5581 tggattataa ggagattgta agaatgtatg attttgataa aatagtcaaa cgtagaaata
     5641 caaaatgtat taaatgggat acttggaaaa aagaaggaaa accgaaagat attttacctt
     5701 tatgggttgc tgatatggat tttgaaacct tgccagaagt tactgaggca attattgaac
     5761 gcgctaaaca tgcagtgtat gggtattcaa tggctggcga tgattattat gaggcggttt
     5821 gtaattggat gaagcgtcgc catcaattag atataaatgc agataatatt gtaacaacaa
     5881 cgggggtagt aacagcgctt aaaatcgcag ttaatgcttt tacagctccc ggtgatgcta
     5941 ttatcattaa taaaccagtg tattatccat ttgatttttc tattgatgaa aatcaacgta
     6001 agaaaattga atgtccgatg atgtttactg gtcaaacata cgaactagat tttgatttgt
     6061 ttgagcagtt gattattgat aatgatgtga aaatgtttat tttatgtaat ccgtataatc
     6121 caataggaaa agtttggaat aaagaagaat tatttaaatt aggtaatatt tgtaagaaac
     6181 ataatgtact ggtagtcagt gatgaaattc atcaagattt tatttataag gggaataagc
     6241 accttccttt tgttaatgtt gatgcctcat ttaaagaatt tacaattatt tgtacagcac
     6301 caagtaagac tttcaatctg gcgggtttac agacgtctaa tatattattt tttaatcata
     6361 aattaaaaga aaagtttatt aaggttaaaa gttcactagg atttccggtg gaaccaacaa
     6421 tttttggtat tgaagcgtgt aaagcagctt ataatcatgg tgataagtgg gttgatgaat
     6481 tagtggctta tcttgatggc aatattaaat atcttgatgg ttttttaaag aaaaaactac
     6541 caatgctcaa aatgattaaa cctcaggggt tatatcttgt ttgggttgat tttagtgctt
     6601 tgcaaatgac gcacgaggaa ttagaagcat ttatggtcaa tgaagctaaa ttatggcttg
     6661 atgaaggata tatttttggt gttggtggag caggttttga gagatttaat ttagcgatgc
     6721 cgcgctgttt attagtgcag gctctagata atttatatca agcgttaaag aatagacagc
     6781 taatataaag atcaccggtg gtggtctttt atttttttgt ttataataaa ttattattga
     6841 cataataata aaatgtaata taataaaacc gtatcaaacc ttgtttgata taatgagcgc
     6901 agatttaata tgttatcttt agagattgat tttttaattg ttctccaaat agtttaataa
     6961 ttcaatcttt tatagataag gagaaaatgt agttgatgtt atgtcccgcc agtagcaaca
     7021 agtttcccac tgctattcat cgtgtatatc tattttttag tgatgtagca tcattgatgc
     7081 tgcattttct ttattttcta aaaaagaaat cgcttacgac tcagataatg gggtaaaaat
     7141 ataggaaata ggcaaaaaaa ctttgtgtta ctatggaaat ttcaattaaa taatagtaat
     7201 atataggagt aaaaagaaaa ggagaaattt tatgggatta gtatcagcaa cagaaatgtt
     7261 aaataaagca aaagaaggac attatgcagt tggtcaattt aacatcaaca acttagaatg
     7321 gacaaaatct attcttttaa ctgcagaaga attaaaagct ccagtaatct taggagtatc
     7381 tgaaggtgct gctaaatata tgactggatt caaaactgta tcagcaatgg tatcagcaat
     7441 ggtagattct ttaggaatta ctgtaccagt agctttacat ttagatcatg gtagctatga
     7501 aggagctaaa gctgctttag aagctggttt ctcttcaatc atgttcgatg gttctcatta
     7561 tggaattgaa gaaaatattg aaaaaacaaa agaaatcgtt gaattatgtc actcaaaagg
     7621 tgtatctgta gaagcagaag ttggatcaat tggtggagaa gaagacggtg tcgttggtaa
     7681 aggtgaagta gctgatccaa aagaatgtaa aatgatcgct gacttaggag ttgacttctt
     7741 agcagcaggt attggaaaca ttcatggtaa atatccagaa aattgggaag gtttagattt
     7801 tgatgcatta gatgcaattc aaaaagaaac tggtaaatta ccattagtat tacatggtgg
     7861 aactggaatt ccagaggata tgattaaaaa agcaattact ttaggtgttt ctaaaatcaa
     7921 tgtaaatact gaatgtcaat tatatttcca agaagcaaca agaaaataca ttgaagctgg
     7981 aaaagattta gaaggtaaag gatttgaccc tcgtaaatta ttggcaccag gtgctgcagc
     8041 tatccaagaa tgtgttaaag aaaaaatgga aatctttggt tgcattggta aagcttaatt
     8101 ataactatag ttaatgggga tttcacatat gtgaaatcct tttttgtctg caataaaccc
     8161 ccagctaggc tgggggtctc aaaaagcttt agcggagtga taatgaaaaa cctcacgtga
     8221 tataatataa atgcggctac caactgcaaa aataagatat cacggaggaa aagacaaatg
     8281 aataaacagt attcagatga cttacatagt ttatcacaca caaaatggag ttgcaaatat
     8341 catattgtat ttgcaccaaa atatagaaga agagcatttt atgaagcaag aggggtagag
     8401 gtaggagcaa tactaagaca attatgtgaa tggaaaggcg taaatataat agaagcggaa
     8461 gtatgcatag atcatgtaca tttgttagta gaaataccgc ccaaatatag tgtttcaggg
     8521 tttatggggt ttttaaaggg aaa
//
LOCUS       DAOMTA010000021         8367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_89_length_8367_cov_2.110563, whole genome shotgun sequence.
ACCESSION   DAOMTA010000021 DAOMTA010000000
VERSION     DAOMTA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_8367_cov_2.110563"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLK68_01200"
     CDS             <1..691
                     /locus_tag="PLK68_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HRM89057.1"
                     /translation="YNVSLQVLDAILCHNGEMLSKKYMPDYDKTIAQFWQEYEDCWHQ
                     KNTSLIIRPMTLEGCVVRVSDVISYIGKDIEDAIKVGIIKQSDLPKEVTDILGFDNKS
                     MINRLIGDIVIHSYGKPYLRFSNEVFSALKTLLNFLSNRVHQHPVLVKENTKLVRMIN
                     QLYHVYYDELTDPNNHDCKIKAFVNKMAPAYSKNDPALIVADYLSMMTDSYVLNEYES
                     IFLPIQHNEIL"
     gene            764..1090
                     /locus_tag="PLK68_01205"
     CDS             764..1090
                     /locus_tag="PLK68_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HRM89058.1"
                     /translation="MKLIIAIVNSDDSSSVQGALTEGGYFVTKLSTTGGFLKKGNTTF
                     FVGTNDDKVEDCISIIKAHSKKRVEKEPTVPPTEMGEFFTPIMVDVLVGGATVFVLNI
                     DQFEKF"
     gene            1146..2807
                     /locus_tag="PLK68_01210"
     CDS             1146..2807
                     /locus_tag="PLK68_01210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM89059.1"
                     /translation="MEYRILKENEIKEAIELVARVAKKDIYKDFDQEGINSFNQVNCD
                     TFYRNKQNLTYICLENKKIIGIITLTQGKHLSLLFVDNDYQGNGIGTKLFELIDNLAL
                     ADLEVNSGIGAKGFYLNLGFKMVGDLTKKNGILYYPMLKQRVVNKRFNTYDEVVNFIN
                     SQKDRVYSLNNFKHYMEDIGNPQLFLDCVHIGGTNGKGSTTNYIKEVLKQAGYRIATF
                     TSPALYSRLDIIRINDQFIDEETMVRYANRYVELWLKYEISMFEIEVFIAIMYFIEQN
                     VDFALFEVGLGGLLDATNIIMPKLAINTNIGLDHVDYLGHDYQSIALNKAGIVKEGID
                     YLTGETKEECLAVFRDVCLKHHSELITLKPITKIIDGNNVSYHYRDYDIILDTPALYQ
                     INNSALALEALIYLKEHQFVDFSDDDLLQGMYNARWAGRFEIINIEPLIIIDGAHNKE
                     GIDAFYECAKKYDNIKIIFSALRDKDYKHMIEKLLQLTKDITICEFEHVRASDAKTLA
                     NGFDVKIEPNFKTAIDKAFTHEGTVFVTGSLYFISKVREYIIDRS"
     gene            2914..3633
                     /locus_tag="PLK68_01215"
     CDS             2914..3633
                     /locus_tag="PLK68_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRM89060.1"
                     /translation="MTLFLLLIAVVVIVCILATRLSSKFGVPTLLIFILLGMVFGSDG
                     LFKISFDDFIISEQICTFALIYIMFYGGFCLNFNSAKPVLIRATIMSTLGVILTCGFV
                     GLFCYWVLHTSLIEGMLIGAVMASTDAASVFSILRMKRLNLKAGIAPLLEMESGSNDP
                     TAYMLTIIILTLLSSGKTNIYMLIFSQIIFGIISGVVIGLLAKNYLDKIQLNEVGLKS
                     IFVAALALFFKCLSIWNNIRK"
     gene            3620..4486
                     /locus_tag="PLK68_01220"
     CDS             3620..4486
                     /locus_tag="PLK68_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRM89061.1"
                     /translation="MLGNSRLKKKVSLVHFFDSISHMMQILLFFLLGLLSFPSQIPDI
                     IGSAILITLFLTFVARPLTVFICLTPFRVPFKQQLFISWCGLRGAASIVFAIMTVVSP
                     AYTNNDIFHIVFFVALLSITFQGSLLPLVAKWLKVEDDQNDTMKTFNDYSDDSSFELI
                     RVYLSDDHPWINQTLIDIVMPTNMLVATIIRDNQMILPKGTTRVLKGDLLIMCAPGYE
                     GNDIYLDEEYIEEHHHWIDCTLAMINPRNKFLVVLIKRDNKMIIPDGKTRIKRDDMLV
                     ICKKEYLGFVDE"
     gene            4677..5471
                     /locus_tag="PLK68_01225"
     CDS             4677..5471
                     /locus_tag="PLK68_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HRM89062.1"
                     /translation="MIQIICGIILVLIGIFVFWKFPIKSDTKSLTLAALFIVLAAILK
                     RLSIMIPLFGFESLKISIEVIPMLLAGIMLAPGYCYIIGLAVDLVGLIVTPTGFPFLG
                     FTLSAVLQCLIPSIVVATIKENYMNYLEKAIQVILIFLGIGACFYVFSLDQVVISKNV
                     VDITLNFKIIISVVCIVMISVLFTVMRYYKKKLNDQEYHLFNLWLISVVLVEMVITFM
                     LTPYWLQVMYGIPFTLSLFIRVIKECIMIPVDIILGYSVLRVLKRL"
     gene            5539..5982
                     /gene="spoVAC"
                     /locus_tag="PLK68_01230"
     CDS             5539..5982
                     /gene="spoVAC"
                     /locus_tag="PLK68_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HRM89063.1"
                     /translation="MNTSQYNEIAEDLKPQKQVGKHCLSAFIYGGIIGAVGQGILEFY
                     MYIFDVSEKEATPMMIITIVLAAAILTGLGIYDRIGKKAGAGTFIPITGFANSMTSSA
                     LEAKSEGLVTGIGANMFKLGGTVITFGIVASFVLGGIRYVISLIW"
     gene            5960..6964
                     /locus_tag="PLK68_01235"
     CDS             5960..6964
                     /locus_tag="PLK68_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HRM89064.1"
                     /translation="MSSVLSGSSMQLNNVYVRATGTTCGIDEFNGPLGQYFDRHYPDF
                     HFGHKSYELAEMAMLQDAISHALKKGKLKKDDINVFLGGDLNNQVTASNYTAKDFQRP
                     FMAMYGACATMGLVINTASMLIENHCIHNANCFVCSHNATAERQFRYPIEYGVQRKET
                     MTTTATGAVSVILDNNPSAVRIEALTIGRVIDMNQSDPNDMGRAMAPAAFDTLVNHLK
                     DLNRQGTDYDLILTGDLSTYGKSIMKELMNENSVTYNEYDDCGCLLYDSDQDVNQGGS
                     GPSCSALVAFGYIYQKMLKHKYKRVLVITTGALLSPVMTNQKQSIPCIAHAFSLEVVE
                     "
     gene            6964..7314
                     /gene="spoVAE"
                     /locus_tag="PLK68_01240"
     CDS             6964..7314
                     /gene="spoVAE"
                     /locus_tag="PLK68_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HRM89065.1"
                     /translation="MNYLLAFIFCGFVCVIAQLIYEYSKLTPGHITSLFVVIGAFLDL
                     FHIYDKLVEIFHAGALLPITSFGHSLMHGALAATKEFGVFGLAMGIFDLTAAGISSAI
                     LFAFLVAICTKPKS"
     gene            7311..>8367
                     /locus_tag="PLK68_01245"
     CDS             7311..>8367
                     /locus_tag="PLK68_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HRM89066.1"
                     /translation="MINNNPSFDLNTRTLDFENEKVTINYVSSLCSDDLIAYLVEGIT
                     NHRGETLKDCLNNGDVKDEPDYQKAMYSMLTGCAMISYRDKTYVLDTRHFPSRSVEEP
                     ETEKSVRGSKDGFNESMLTCAGLIRRRIRTIDLVMNKQTIGKNNPLDVCLCYLDSTVD
                     QTMLETVLQRIKEIKNEDLVMSDRAVEEMILDQGYNPFPLVRYSERPDVVATHILHGH
                     IAIICDTSSSVMMLPTTLFEILEHVEEHRQTPIIGTFIRLIRCSAVLISIYLVPLWLL
                     LTSKGTIDLVFLGQVLLVELAIELLRIATIHTPTSLSNAMGMIAAVLLGQFAIDLGIF
                     SEEILLFCAIGDVGGFAT"
BASE COUNT         2722 a         1010 c         1624 g         3011 t
ORIGIN      
        1 ttataatgtc tctttacaag tgttagatgc gattctgtgt cataacggtg aaatgttatc
       61 gaaaaaatat atgccggatt atgataaaac tattgcgcaa ttttggcagg aatatgaaga
      121 ttgttggcac caaaagaata ctagcttaat tattcgtcca atgacattag aaggctgtgt
      181 tgttcgggtg tcagatgtta tctcatatat tggtaaagat attgaagacg caattaaagt
      241 aggaatcatt aaacagtctg atcttcctaa agaggtaact gatattttag gttttgataa
      301 taaatcaatg ataaatcgat tgattggaga tattgttatt catagttatg gaaagccata
      361 tcttcgcttt tctaatgaag tgtttagtgc tttgaaaact ttattgaatt ttttatccaa
      421 tcgggttcat caacatccgg tgctagtaaa agaaaataca aaattagtcc gaatgattaa
      481 tcagttatat catgtttatt atgatgaatt aactgatcct aataatcatg attgtaaaat
      541 aaaagcattt gttaataaga tggctccggc atatagtaaa aatgatccag cattaatagt
      601 tgctgactat ttatcaatga tgacagattc ttatgtttta aatgaatatg aatctatctt
      661 cttacctatt caacataatg aaatacttta attattatat ttgtactatc ttttgaaaaa
      721 tcgtagtata atatatgtat aagaagtata aggaggagtt aatatgaaac ttataatcgc
      781 aattgttaat tcagatgaca gctcatcggt tcaaggagct cttactgaag gcggatactt
      841 cgtaactaaa ttatctacta ctgggggatt tttgaaaaaa ggaaatacaa cattttttgt
      901 tggaactaat gatgataaag tagaagactg tatttctatt atcaaagcac attcaaagaa
      961 acgggtagaa aaagaaccta ctgttcctcc aacagagatg ggagaattct ttacaccaat
     1021 tatggttgat gttttagttg gtggggcaac cgtatttgtt ttaaatattg atcaatttga
     1081 aaaattctaa aaaaagaagt ggcttagcca cttcttttat tctataatgt tctaaggtgg
     1141 agaatatgga atatagaata ttaaaagaaa atgaaataaa agaagcaatt gaattagttg
     1201 cacgagtagc taaaaaagat atttataaag actttgatca agagggaatt aatagtttta
     1261 atcaagtaaa ttgtgataca ttttatcgta ataaacaaaa tttgacttat atatgtttag
     1321 agaataaaaa aataattggg attattacac ttactcaggg aaaacatcta agtttattat
     1381 ttgttgataa tgattatcag ggaaatggga ttggaacaaa attatttgag ttgattgata
     1441 atttggcttt agctgatctt gaagttaatt ctggaattgg ggctaaagga ttttatttaa
     1501 acttaggttt taaaatggtc ggtgatctaa ctaagaaaaa tggtatatta tattatccaa
     1561 tgcttaaaca aagggtagtc aataaacgtt ttaatactta tgatgaggta gttaatttta
     1621 taaatagtca aaaagatcgg gtatacagcc ttaataattt taaacattat atggaagata
     1681 taggaaatcc tcagttattt cttgactgtg ttcatattgg tgggacaaat ggaaagggtt
     1741 caactactaa ttatattaaa gaagttttaa aacaggcggg atataggatt gcaacattta
     1801 cctcaccagc actatactca agattagata ttattcgtat caatgaccag ttcattgatg
     1861 aagaaacaat ggttagatat gcaaatcgat atgtggaact atggttaaaa tatgaaatct
     1921 caatgtttga aattgaagta tttattgcaa ttatgtattt tattgagcag aatgtcgatt
     1981 ttgcgttatt tgaagtagga ttaggcggtt tattggatgc aacgaatatc attatgccta
     2041 aattagcaat caatactaat attggtttag atcacgttga ttatttaggt catgattatc
     2101 aaagtattgc tttaaataaa gctggaattg ttaaagaagg aattgattac ttaaccgggg
     2161 aaacaaagga agaatgtttg gctgtattta gagatgtttg tttaaaacat catagtgagc
     2221 ttataacttt aaaaccaata actaaaatta ttgatgggaa taatgttagt tatcattatc
     2281 gtgattatga tattattctt gatacaccgg ctttatatca gattaataat agtgctttag
     2341 cactagaagc actgatatat ttaaaagaac atcaatttgt tgattttagt gatgatgatt
     2401 tacttcaggg aatgtataat gctcgctggg ctggtagatt tgaaataatt aatattgaac
     2461 ctttgattat tatagatggt gctcataata aagaaggaat tgatgctttc tatgaatgtg
     2521 ctaaaaaata tgacaatata aaaataatct ttagtgcttt acgggataaa gattataaac
     2581 atatgattga aaaattgtta caattaacta aagatataac tatttgtgaa tttgaacatg
     2641 ttcgagctag tgatgctaag acattagcaa atggctttga tgttaaaatt gagccaaatt
     2701 ttaaaacagc gattgataag gcttttactc atgaggggac agtatttgta acgggatcgt
     2761 tatatttcat ctctaaggta agggaatata taattgatag aagttagaat caggtaatac
     2821 tgattctttt tttgataaaa aaatttcata atgagtttta tttattattt ttaattagat
     2881 atgatataat aatacaaaga taggaggaat gacatgacgt tatttttatt attgattgca
     2941 gtagtagtaa ttgtgtgtat tcttgcaaca aggctttcat ctaaatttgg agtaccaact
     3001 ttattgattt ttattttgtt ggggatggta tttggaagtg atggcttgtt taaaatcagc
     3061 tttgatgatt ttattataag tgagcaaatt tgtacatttg cattaattta tattatgttt
     3121 tatggtggtt tttgtttgaa ttttaatagt gctaaaccgg tacttattag agcaacgata
     3181 atgtcgacac ttggcgttat tttaacttgt ggctttgttg gtttattttg ttattgggta
     3241 ttgcacacta gtttaattga agggatgctg attggagcag taatggcttc aactgatgcg
     3301 gcttcagtat ttagtatttt aagaatgaag cgtttaaatc taaaagcagg tattgctcca
     3361 ttgcttgaaa tggaaagtgg aagtaatgat cctacagcat atatgctgac aattattatt
     3421 ttgacccttt tatcaagcgg gaaaactaat atttatatgc ttatttttag tcagataata
     3481 ttcgggatta ttagtggtgt agtaattggt cttttagcaa aaaattattt agataagatt
     3541 caattaaatg aagttggctt aaaatcgatt tttgtagctg ctctagcatt gttttttaag
     3601 tgtttatcta tttggaataa tattaggaaa tagtcgttta aaaaagaaag tttcactagt
     3661 tcattttttt gacagtattt ctcatatgat gcagatcttg ttgtttttct tactaggttt
     3721 attgtctttc ccgtcacaaa ttcccgatat aataggctct gctatcctaa ttacactatt
     3781 tttaactttt gttgcgcgtc cgttaacggt atttatctgt ttaacgccgt ttagagtacc
     3841 gtttaaacaa caattgttta tttcctggtg tggtttaaga ggtgcggctt cgattgtttt
     3901 tgcaatcatg acagtggtat cacctgcata tacgaataat gacatttttc atattgtctt
     3961 tttcgttgct ttactttcaa taacatttca agggagtcta ttgcctttag tagcaaaatg
     4021 gctcaaagtg gaagatgatc aaaatgatac aatgaaaaca tttaatgatt atagtgatga
     4081 tagctcattt gaattaattc gagtttattt gagtgatgat catccatgga taaatcaaac
     4141 attaattgat attgtaatgc cgactaatat gttagttgct acaattattc gcgataatca
     4201 gatgatctta cctaaaggga caacaagggt tttaaaaggt gatctattga ttatgtgtgc
     4261 accaggttac gaaggaaatg atatttattt agatgaggaa tacattgaag agcatcatca
     4321 ttggatcgat tgtactctag cgatgatcaa tcctcgtaat aagtttttag tagtcttaat
     4381 taaacgtgat aataaaatga ttatccctga tggtaaaaca cggataaaac gtgatgatat
     4441 gcttgtgatt tgtaaaaagg agtacttagg ctttgttgat gaataattaa atatgttgaa
     4501 gtgtctgcta atttgtagta ttatttccat tgtatacaat cttgtatata atttagaaaa
     4561 agggggagtc tactttttag tagcaatcgc atgtacggtc agtgatcgga attaacgcct
     4621 gtggactaga aatgaggttg aagcaggaaa gattgtaaat aagtagcaat caagttatga
     4681 ttcaaattat ttgcggaatt attttagttt taattggaat ttttgtattt tggaaatttc
     4741 caattaagag tgatactaaa tcactcacat tagcagcttt atttattgtt ttagccgcta
     4801 ttttaaaaag gttatcgatt atgatacctt tgtttggttt tgagagtcta aaaatctcaa
     4861 ttgaagtaat cccaatgctt ttagcaggga taatgcttgc accaggatat tgttatataa
     4921 ttggtcttgc tgttgattta gttggattaa ttgtgacacc aacgggattt ccttttttag
     4981 gatttacttt gagtgcggta ttacaatgtt tgattccatc cattgtagtt gccacgatta
     5041 aagaaaatta tatgaattat ttagaaaagg caatccaagt aattttaatt tttttaggaa
     5101 taggggcttg cttttatgta tttagtttag atcaagtagt tatatctaag aatgttgtag
     5161 acattacact taattttaaa ataataatta gtgtggtttg tattgtgatg ataagtgtct
     5221 tgtttactgt tatgcgttat tataaaaaga aattaaatga tcaggaatat catttgttta
     5281 atctgtggtt gatttcggta gtattagttg aaatggtaat tacatttatg ttaacgcctt
     5341 attggttaca agttatgtat ggaattccat ttacattatc gttatttatc cgagtcatta
     5401 aagaatgtat catgattcca gtagatatca ttttaggata tagtgtttta agagtgttaa
     5461 aacgcctata agagtcagtt ttgactcttt tttgtttaat tttttttaat atggaaaaaa
     5521 tattgtgagg tgaataatat gaatacatca cagtataatg aaattgctga agatttaaaa
     5581 ccccaaaaac aagtggggaa gcattgttta agtgcattta tttatggtgg cattatcggt
     5641 gctgtcggtc aaggcatttt agagttttac atgtatattt ttgatgtgag tgaaaaagaa
     5701 gcaacaccaa tgatgattat tacgattgtt ttggctgctg ctattttgac aggattggga
     5761 atatatgatc gaattggaaa aaaggcagga gctggaactt ttatcccaat tactggattt
     5821 gctaatagta tgacttctag tgccttagaa gctaaaagcg aaggtttagt aacaggtata
     5881 ggagctaata tgtttaaatt aggaggaaca gtcattactt ttgggattgt tgcttcgttt
     5941 gtgttaggag gtattcgtta tgtcatcagt cttatctggt agttcaatgc agttgaataa
     6001 tgtttatgtt agagcaacag gaacaacgtg cgggattgac gaatttaatg gaccgttagg
     6061 tcaatatttt gatagacact accctgattt tcattttgga cataaatctt atgaattagc
     6121 agaaatggca atgcttcaag atgcgattag tcatgcttta aaaaaaggaa agttgaaaaa
     6181 agatgacatt aatgtctttt taggtggaga tttaaataat caagtcactg ccagtaatta
     6241 cactgctaaa gattttcaaa gaccatttat ggcgatgtat ggtgcttgtg caacaatggg
     6301 gctagttatt aatacagcct caatgttaat tgaaaatcat tgtattcata atgcaaattg
     6361 ttttgtttgc agccataatg caaccgctga aagacaattt cgttatccaa ttgaatatgg
     6421 tgtccaacgt aaagaaacaa tgacaacgac agctacaggt gcagtcagtg taattcttga
     6481 taataatcct agcgctgtta gaatagaagc attaactatt ggtcgtgtta ttgatatgaa
     6541 ccaaagtgat cctaatgaca tgggaagagc aatggcacca gcagcctttg atacattagt
     6601 taatcatcta aaagatttaa accggcaagg aactgattat gatttgattc ttactggtga
     6661 tttatcaact tatgggaaat cgattatgaa ggaattaatg aatgaaaata gtgttaccta
     6721 taatgagtat gatgattgcg gctgtctatt atatgatagt gatcaagatg ttaatcaagg
     6781 tgggagtggg ccttcgtgca gtgctttagt agcatttggg tacatctatc agaaaatgtt
     6841 aaaacataag tacaagcggg ttttagtgat tacaacaggg gcattattgt caccggttat
     6901 gactaatcaa aaacaaagta taccttgtat tgcccatgcc tttagtttgg aggttgttga
     6961 ataatgaatt atctattagc atttattttt tgtggctttg tctgtgtaat tgcacagttg
     7021 atttatgaat attcaaaatt aactcctgga catattacga gtctatttgt tgttattggt
     7081 gcttttttag atttgtttca tatctatgat aaactagttg aaatatttca tgcaggagca
     7141 ttattgccaa tcacaagttt tggtcattcg ttgatgcatg gagcgcttgc agcaactaaa
     7201 gaatttggcg tttttggatt agcgatgggt atttttgatt taaccgcagc gggtattagt
     7261 agtgctattt tatttgcttt tttagttgca atttgtacta agccaaaatc atgatcaata
     7321 acaatccatc atttgattta aatacccgaa cgcttgattt tgaaaatgaa aaagttacaa
     7381 ttaattatgt ttctagtctt tgctctgatg atctaattgc ttatttagtt gaagggatta
     7441 ccaaccatcg tggtgaaaca ttaaaagatt gcttaaataa tggtgatgtt aaagacgaac
     7501 cggattatca aaaggccatg tattcaatgt taactggttg tgcaatgatt agttatcgcg
     7561 ataaaactta tgttttagat actcgtcact ttcctagtcg ttctgtagaa gaacccgaga
     7621 cagaaaaaag cgtcagaggc tctaaagatg gttttaatga atcgatgctg acttgtgcgg
     7681 gtttgattcg ccgacgaatt cgtacgattg atctagttat gaataaacag acgattggga
     7741 aaaataatcc cttagatgtt tgtttatgtt atttagatag cacagttgat caaactatgc
     7801 tggaaacagt attacaacgt ataaaagaaa ttaaaaatga agatttggtc atgtctgatc
     7861 gtgccgttga agagatgatc ttagatcaag gatataatcc atttccactt gttcgatata
     7921 gtgaacgccc ggatgtagta gctacacata tccttcatgg tcatattgct attatttgcg
     7981 atacatcgtc ttcagtaatg atgttgccga ccacattatt tgaaatattg gagcatgttg
     8041 aggagcatcg tcaaacacca atcattggga cttttatacg attaattcgt tgtagtgcag
     8101 tattaatttc tatttattta gtacctttat ggctactatt gacaagtaag gggactattg
     8161 atctagtctt tttaggacaa gtcttgcttg ttgaattagc gattgagttg ttacggattg
     8221 ctacgattca tactcctacc tctttgtcaa atgcgatggg gatgattgcc gctgtattat
     8281 taggacagtt tgcaattgat ttaggaattt ttagtgaaga aattttatta ttctgtgcaa
     8341 ttggtgatgt cggtggtttt gcaaccc
//
LOCUS       DAOMTA010000022         8279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_91_length_8279_cov_2.378648, whole genome shotgun sequence.
ACCESSION   DAOMTA010000022 DAOMTA010000000
VERSION     DAOMTA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_8279_cov_2.378648"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLK68_01250"
     CDS             <1..563
                     /locus_tag="PLK68_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM89067.1"
                     /translation="ITDDMLEDEIADFKENGTRAEVVKRTEAELFELYKDPNLDYKPE
                     QLTKRGGTYYSDAACELINSIYNDKRTTMVVSTQNKGALTDLPYDSVAEVSAIITAAG
                     PMPISWGTLKPAARGMVQIMKAMEETTIEAAVTGDYGKALHAFTINPLVPSGQIAKTL
                     LDEMLIAHKKHLPQFADKIAELEAQQ"
     gene            complement(648..2075)
                     /locus_tag="PLK68_01255"
     CDS             complement(648..2075)
                     /locus_tag="PLK68_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRM89068.1"
                     /translation="MEKSDGYELCYGIGDYGQHTYTMTYTITPFVNQYSDAQGFNWQL
                     LNQEMNPKPAEFEATISSDYKFYDQDSDIWGFGYEGQVIFDDHGAIIISNRDLNNNRR
                     GDINYVNILVRVPDGTFSNAITKNESFEAVLEDARDGSSYQEDSSDGSIFMFIGISVA
                     VLAGIIAVIVGVVSKANKNKQLYFSDGNNEIPTMEHVNPFRDIPCHKDIYYFYYVATK
                     AGIIQQDDRSGILCAMLLKWIRDGYVNFTMEPSTGWFKKDKYEIDFSGEIPTENILEE
                     KMLGYFREASGDNQILENKEFERWCKRNYEEIEDWFNDLIEFEEKELKEKGLMKKQTT
                     YKKFLGIDIANEKVVYEPSFRDDIIYTKGLKRFLLDFSSIEEKEVIEVKLWEEYLMFA
                     SILGIADEVEKQIGKLCPEFNQYSNIDYTYTMLATRTFMYDGVRSAANAYSAAHSSSY
                     SGGGGFSSFGGGGGGFSGGGGGGSR"
     gene            complement(2136..2393)
                     /locus_tag="PLK68_01260"
     CDS             complement(2136..2393)
                     /locus_tag="PLK68_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89069.1"
                     /translation="MKLKKLFLALSIFLVSLMPLPVLASGNDLQAVNMNIYINQDGSA
                     KVEETWKMDAIEGTENYKAFNNLYGATISDFSVVDEKGTKY"
     gene            complement(2395..2949)
                     /locus_tag="PLK68_01265"
     CDS             complement(2395..2949)
                     /locus_tag="PLK68_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRM89070.1"
                     /translation="MSIPIIIVLIVVALIVIYCIVAYNTLTKLKNRVKTNWAQIDVVL
                     KRRADLIPNLVETVKGYASHEKETLENAIKARNTYVTANSPQDQLAASGELNQALSRL
                     MMLGEAYPDLKANTNFMELQKELTATEDKITYARQFYNDSVNKYTNQTEVFPSVLIAK
                     IFGFGTFDYFEVQETDKEVPKVKF"
     gene            complement(3078..>7322)
                     /locus_tag="PLK68_01270"
     CDS             complement(3078..>7322)
                     /locus_tag="PLK68_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM89071.1"
                     /translation="HPYGYTAFAFDLTEGLICDGETENVLVVKTNNKIPSSRWYSGSG
                     IYRDVKLTVTDKVHVGYNGTKIIAKDLENTKDNNVKVDVTATIDNDSDTNKTITVKNT
                     LLDAQGNAAGSTVTNEVELAAKSTKDVKQEVSVIAPKLWSTEDTNLYTMKTEIVADNK
                     VLDTYETEYGFRYFDFDNNTGFSLNGEKIKLKGVCLHHDQGALGAVANRDAIERQVKI
                     LKEMGCNAIRVTHNPAASVLLEVCNENGMLVINEAFDGWTEYKNGNVNDYTSHFNEVI
                     STDNQIVNGKAGMKWGEFDVKAMVDGAKNDPSVIMWSLGNEIDEGVSGNTSHYLNLVD
                     DLIKWVQEVDTTRPVTNGDNRKNTNPNAMLSQINQKIYEAGGVVGMNYANGDQTIAMH
                     NTYSDWPLYGSETASAVHSRGVYNTTGKDDNTLQMSEYDNDEAKVGWGHSASDAWQFV
                     IKNDFNAGEFVWTGFDYIGEPTPWNGTGAGSASGQGAKPKSSYFGIVDTAGFVKDTYY
                     LYKSMWDEDTTTLHLMSTWNNDEIAKNNNGKVKVDVFTNAAKVELYLNGNKVGEDTAT
                     THTTALGYKYQTFSNGEFYPSFNVTWASGTLSAKAYDKKGNLITETEGRSSVTTNTKA
                     AKLAISADKKEILADGSSLSYLTVDVKDMNGNIVAGADNRINFKIEGNGKIVGVDNGN
                     AADTDSYKGTSRKAFSGKALVIVQSTKDEGSFTLSASSDGLSGSNISVKTVKNAVEGD
                     AYLQSYTIAKNLYVNVGEEPDLPAKVVGTYNNGETRDLTIKWNEYDKESLNKIGEFTI
                     TGKLQDSEAVVTVTVHVIGNVVAMENYSTVTSAGITPVLPQTVRGLYENGNYSEAFPV
                     KWNIPADAFKNEGMVTVKGTVNVLNEVKDVTATVRVAPKLADSQNIASKNYQDTPLFT
                     NGKMVNGVPSEPTATPINDSLSELNDGITNESGRESARWTNWSLRNENPPVDTYVQLE
                     WQKEYLMQNIKLWHFTDNQFSVLPGDNNVRFEYYDVPTNSWQEIESSHITQVPYTSGD
                     TPYGFIHPITTNKLRIWMKSPQVNKCIGLTEIEVYDYILPVTANSSANLDDLKLDGVN
                     IKEFNGYRGYDKLTKTYTVDLKTADTPSVIAQGNNNEAITVLPVYNNEVKIIVRAEDG
                     KTIENYTIKYIISVNKEFLENYIESDEIKKVFETSEEYTVQSYQEFKEAYDQAIVILK
                     DDQATQKQVDDSYEKLQTMYQALVKKTEEKVDKSQLEALLAIADKITEDILSNLDADL
                     VKEFKTALQAAQDIYTGNDVTQAQVNAAITRLNVAIENLNIRDFNKIKLQELVERTEK
                     LSAKDYTRASWERLEKALSAAKIVLADEKATLAAVDEAKTVLQNALEQLEKVAISEES
                     SQGVKTGDAANFAGIFALMVSTASLVLWMKRRKENN"
     assembly_gap    7323..7343
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7344..7762)
                     /locus_tag="PLK68_01275"
     CDS             complement(<7344..7762)
                     /locus_tag="PLK68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89072.1"
                     /translation="MKGRWNQKLCAVATVSMLLSSAPVGVLAQENARITQSDPEEVYV
                     DIIGDGQRTTLFNENWKFHRGDINDAQNKDYNDSTWETVNLPHDYSIDQDFTTSGEAE
                     SGFLPGGVGWYRKTFVVPKKYQEKQLMIEFDGAYMNA"
     gene            complement(8022..>8279)
                     /locus_tag="PLK68_01280"
     CDS             complement(8022..>8279)
                     /locus_tag="PLK68_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89073.1"
                     /translation="LDQLLINEKAVEIKDNSYMIKNVTADIRVRASFKKAGEVTVKPD
                     ESKDSSVVATGDEVQLFGYIGMFIGALVLLAELKRKFKHQS"
BASE COUNT         2543 a         1701 c         1065 g         2949 t
ORIGIN      
        1 atatcactga tgatatgctt gaagatgaaa ttgccgactt caaagaaaat ggtactcgtg
       61 ctgaagttgt aaaaagaaca gaagctgaat tatttgaatt atataaagat cctaatttag
      121 attataaacc agaacaattg acaaaacgtg gtggtactta ttatagtgat gctgcttgtg
      181 aattaatcaa ctcaatctat aatgataaac gtacaactat ggtcgtatca acacaaaaca
      241 aaggggcatt aactgatctt ccatatgata gtgtagctga agtatctgct atcattacag
      301 cagcagggcc aatgccaatt tcttggggaa cattaaaacc tgcagctcgg ggaatggttc
      361 aaattatgaa agcaatggaa gaaactacaa ttgaagccgc cgttactggt gattatggta
      421 aagcattaca tgcatttaca attaatccat tagttccaag tggtcaaatt gctaaaacat
      481 tattagatga aatgttgatt gctcataaaa aacacttacc tcaatttgct gataaaatcg
      541 ctgaattaga agcacaacaa taaaataacc tggagttttg aagcgaacct cctaagttga
      601 agcaacaact taggaggttc atttcattct tccaggtttt ctttatttta tcggcttccg
      661 cctccgccgc ctccggagaa accacctccg cctccgccaa aggaactaaa accaccgcca
      721 ccgctatacg atgaactatg agctgcacta tatgcattag ctgcactacg aacaccatca
      781 tacataaatg ttctcgttgc taacatcgta taagtgtaat caatatttga atattgatta
      841 aattctgggc agagtttacc aatttgtttt tctacctcgt cagcaatacc aagaattgaa
      901 gcaaacatta aatattcttc ccagagtttt acttcaatta cttctttttc ttcaatacta
      961 ctaaagtcta ataaaaagcg ttttaatccc ttagtataaa tgatatcatc tctaaatgat
     1021 ggttcataaa ctactttttc atttgcaata tctattccta agaacttttt ataagttgtt
     1081 tgctttttca ttaagccttt ttcttttaat tctttttctt caaattcaat caaatcatta
     1141 aaccaatctt cgatttcttc ataattccgt ttacaccaac gttcaaattc tttattctct
     1201 aagatttgat tatcaccact tgcttcccga aaataaccta acatcttttc ttctaaaata
     1261 ttttctgttg gaatctcacc actgaaatca atttcatatt tatctttttt aaaccagcca
     1321 gtgctcggtt ccatggtaaa attcacatag ccatctctga tccattttaa caacatagca
     1381 cacaagatac ctgaacgatc atcttgctgg ataatccctg cttttgttgc aacgtaataa
     1441 aaatagtaga tatctttatg acaaggaata tcacggaacg gattaacatg ttccattgtc
     1501 ggaatttcat tgttaccgtc actaaaatat aattgtttat ttttgtttgc tttagataca
     1561 actccaacaa ttactgcaat tatcccagca agtaccgcaa cactaatacc aataaacata
     1621 aatattgaac catcactaga atcttcttga taacttgagc catcacgtgc atcttctaaa
     1681 acagcttcaa atgattcatt tttggttatt gcattgctaa aagttccatc gggtactcta
     1741 actaaaatat taacataatt aatatcgcct cgacgattat tgtttaaatc acgattcgat
     1801 ataattatcg ctccatgatc atcaaaaata acttgtcctt catagccaaa gccccaaata
     1861 tcactatctt gatcataaaa cttgtaatca gaactaatcg ttgcttcaaa ttcagctggt
     1921 ttaggattca tttcctgatt aagtaattgc caattaaacc cctgagcatc actatattga
     1981 ttaacaaatg gagtaatcgt ataagtcatc gtatatgtgt gttgaccata atcacctatc
     2041 ccataacata actcataacc atcactcttt tcaatgatcc cacatttatt tttcttctct
     2101 tgacgactag catcaatatc ccaattatca acatattaat attttgttcc tttttcatcg
     2161 acaacagaaa aatcactgat cgtagcccca taaagattat taaatgcctt gtagttttct
     2221 gttccttcaa tcgcatccat cttccaagtt tcctcaactt ttgctgaacc atcttgatta
     2281 atataaatgt tcatattaac cgcttgaagg tcattccctg atgccaaaac aggtaacggc
     2341 atcaaagaaa ccagaaagat tgagagtgct aaaaataatt ttttaagttt cattttaaaa
     2401 tttaaccttt ggtacttctt tatctgtttc ttgaacctca aaataatcaa aagttccaaa
     2461 accaaatatt tttgcaataa gcactgaagg gaaaacttct gtttgattgg tatatttatt
     2521 tactgaatca ttataaaatt gtcgtgcata cgtaatttta tcttctgtag ctgtcaattc
     2581 tttttgtaat tccataaaat tagtattagc ttttaaatcc ggataagctt cacctaacat
     2641 cattaatcgg cttaaagcct gatttaattc accagaagct gcaagctgat cttgaggact
     2701 attggcagtc acataagtat ttcttgcttt tattgcattt tctaacgttt ccttttcgtg
     2761 actagcatac cccttgactg tttcaacaag gttcgggatc aaatcagccc gcctttttaa
     2821 taccacatca atttgtgccc agttagtttt aacccggttc tttaatttag ttaatgtgtt
     2881 atatgcaact atgcagtaaa tcacaattaa cgccactact ataagtacaa taattattgg
     2941 tatactcatt tttaaacgcc tcctttttct atacttatat tatacaatct tagtaagaat
     3001 atacaacgaa tacgataatc ataaaaatgt caaatttcca aaaaaggatg tcatattaaa
     3061 acatccttct attattatta attattttct tttcttcttt tcatccataa aaccagactc
     3121 gcagttgaaa ccattaatgc aaatatacca gcaaaattag ccgcatcacc agtttttact
     3181 ccttgagaag actcttcgct aatcgcaact ttctctaatt gttctagtgc attttgtaaa
     3241 accgttttgg cttcatcaac agctgccagt gtcgcctttt catctgctaa gacaattttg
     3301 gctgctgata aagctttttc taatcgttcc caagaagccc ttgtataatc tttagcagat
     3361 aatttttcag tccgctcaac tagctcctgt aatttaatct tattgaaatc acgaatattt
     3421 aaattttcaa ttgccacatt taaccgtgta atagcagcat ttacctgagc ttgagttaca
     3481 tcatttccag tataaatatc ttgtgctgct tgtaaagcgg tcttaaactc tttaactaaa
     3541 tcagcatcaa gattacttaa aatatcttca gtaatcttat cagcaatagc taaaagtgct
     3601 tctaactgac tcttatcaac tttttcttca gtctttttaa ctaaagcttg atacatagtt
     3661 tgtaattttt cataactatc atcaacttgt ttttgtgttg cctgatcatc tttcaaaatc
     3721 actattgctt gatcatatgc ttccttaaat tcttgatacg attggactgt atattcttca
     3781 cttgtttcaa aaactttctt tatttcatcg ctttcaatat agttttcaag aaactctttg
     3841 tttaccgaga taatatattt aatagtataa ttttctatcg ttttaccatc ttccgctcta
     3901 acaatgatct ttacttcatt attatatact ggtaaaacag taattgcttc attattatta
     3961 ccttgagcta taactgatgg ggtatcagct gttttcaaat caacagtata tgttttagta
     4021 agcttatcat accctcgata accattaaat tctttaatat taaccccatc taattttaaa
     4081 tcatctagat ttgcagatga atttgcagtt actggtaaaa tataatcata tacttctatt
     4141 tctgttaaac caatacattt attaacttgc ggtgacttca tccatatgcg taatttatta
     4201 gttgtaatcg ggtgaataaa tccatatgga gtatcaccag atgtatatgg tacttgtgta
     4261 atgtgcgaag actcaatttc ttgccatgaa tttgttggaa catcataata ttcaaaccgg
     4321 acattgttat cacctggtaa aacactgaat tgattatccg taaaatgcca cagtttaata
     4381 ttttgcatca agtattcttt ttgccattct aattgtacat aagtatccac cggaggattt
     4441 tcatttctta aactccaatt tgtccaccgt gcgctttcgc gtccactttc attagtaatc
     4501 ccatcattta attcactcaa tgaatcattg attggcgttg ctgttggttc acttggaaca
     4561 ccattgacca ttttcccatt tgtaaataat ggtgtatctt gataattctt tgaagcaata
     4621 ttctgtgaat cagctaattt tggtgctact cgaacagttg ctgtaacatc ttttacttca
     4681 tttagaacat tgactgtacc ttttactgtt accattcctt catttttaaa agcatcagca
     4741 ggaatattcc atttaaccgg aaaagcctca ctataatttc cattttcata aagtcctcga
     4801 accgtttgtg gtaataccgg tgtaattcct gcactagtta ctgttgaata gttttccatg
     4861 gctacgacat taccaattac atgaaccgtt acagttacta cagcttcact atcctgaagt
     4921 tttcctgtta ttgtgaactc accaatttta tttaatgatt ctttatcata ttcattccat
     4981 ttaatcgtta agtctcttgt ctccccatta ttgtatgttc ctacaacttt tgcaggcaga
     5041 tcaggttctt caccaacatt aacatataaa tttttagcaa ttgtgtaact ttgcagataa
     5101 gcatcaccct caacagcatt tttaaccgtc ttgacagaaa tatttgaacc acttaaacca
     5161 tcactgctgg cactaagtgt aaatgatccc tcatctttag tagattgaac aatcactaat
     5221 gcctttcctg aaaaggcttt tcgtgaagtt cccttataac tatcagtatc agctgcatta
     5281 ccattatcaa ccccaacaat tttaccattt ccttcaatct tgaaattaat acgattatca
     5341 gctccagcga caatatttcc attcatatct ttaacatcga cagtaagata agataaacta
     5401 ctgccatcag ctaaaatttc ctttttatca gcacttatcg ctaattttgc agctttggta
     5461 ttagtcgtta cactgcttct tccttcagtt tctgtaatta aattaccttt tttgtcatat
     5521 gctttagcac tcaacgttcc tgaagcccag gttacattaa agcttgggta aaattctcca
     5581 tttgaaaatg tttgatactt atatcctaaa gcagtagtat gcgttgttgc tgtatcttcc
     5641 cccactttat tcccatttaa atataactcc acttttgcag cattagtaaa tacatcgacc
     5701 ttgaccttac cattattatt tttggctatt tcatcattgt tccatgttga cattaaatgc
     5761 aaagtagttg tatcttcgtc ccacatactt ttatataaat aataagtatc ttttacaaat
     5821 cctgcagtat caacaattcc aaaatatgat gatttaggtt tagctccctg accacttgca
     5881 cttcctgctc cagttccatt ccatggcgtt ggttcaccaa tataatcaaa accagtccac
     5941 acaaactctc cagcattgaa atcattctta ataacaaatt gccaggcatc acttgctgaa
     6001 tgtccccagc caacttttgc ctcatcatta tcatattctg acatttgtaa agtattatca
     6061 tctttacccg tagtattata aactccacga ctatggacag cacttgccgt ttcagaaccg
     6121 tatagtggcc aatcgctata agtattgtgc atagctatag tttgatcacc atttgcatag
     6181 ttcattccaa ccacgccacc agcttcatat attttttgat taatttggct gagcatcgca
     6241 tttggattag tatttttgcg attatcacca tttgtgactg gacgagttgt atcaacctct
     6301 tgaacccatt taattaaatc atcaactaaa tttaaataat gtgaggtatt tcctgatact
     6361 ccttcatcaa tttcattacc taatgaccac ataattacac ttggatcatt tttagcacca
     6421 tcaaccattg ctttaacatc aaattcgccc cacttcattc cagcttttcc attaacaatt
     6481 tgattatcgg tactaattac ttcattaaaa tgtgaagtgt agtcattaac atttccattt
     6541 ttatattcgg tccagccatc aaaagcttca ttaataacta acattccatt ttcattacat
     6601 acttctaata aaactgatgc cgcaggatta tgggtaacac gaatcgcatt acatcccatt
     6661 tcttttaata ttttgacctg ccgttcaatt gcatcacgat tagcaactgc tcctagtgca
     6721 ccctgatcat ggtgcagaca aacacctttt aattttattt tttcaccatt taatgaaaaa
     6781 cctgtattat tatcaaaatc aaaatagcgg aaaccatact cagtttcata agtatctaaa
     6841 actttattat ccgccacgat ttcagttttc attgtatata aatttgtatc ctcagtggac
     6901 cacaatttag gagcaataac agacacttcc tgttttacat cttttgtact tttagctgct
     6961 aattcaactt cattagtgac ggtactaccc gcggcatttc cttgagcatc caataaagta
     7021 tttttaactg tgatcgtttt atttgtatcg ctatcattat cgattgttgc tgtaacatca
     7081 actttaacat tattgtcctt agtattttct aaatctttag caatgatttt agttccatta
     7141 tagccaacat gaaccttatc tgtaactgtt aactttacat ctcgataaat cccacttcca
     7201 gagtaccagc gactacttgg aattttatta ttagttttta ctactaaaac attttcagtc
     7261 tcaccatcgc agattaagcc ctcagtcaaa tcaaatgcaa aagcagtata tccatatgga
     7321 tgnnnnnnnn nnnnnnnnnn nnntcagcat tcatataagc gccatcaaac tcaatcatca
     7381 attgtttttc ttgatatttt ttaggtacta caaaagtttt tcgataccag ccaacgccac
     7441 ctggtaaaaa accactctct gcttctccgc ttgttgtgaa atcttgatca atactatagt
     7501 catgtggtaa attcaccgtt tcccaagttg aatcattgta atctttgttt tgagcatcat
     7561 ttatatcacc acgatgaaac ttccaatttt cattaaatag cgtggttctt tgcccatctc
     7621 caatgatatc aacatatact tcttcggggt ctgactgagt aattcttgca ttttcttgtg
     7681 ctaatacccc aactggtgct gatgataaca acattgaaac agttgctaca gcacataatt
     7741 tttgattcca tcttcctttc ataacttcct ccttttcccg tatagaatag ctattttgat
     7801 tagttaaagt tcccaaataa atataaaaga cctgattgtt catcacgctt ataaacgctt
     7861 taaacaacca ggtcttgcct aatcaaatag taacaatcct atgcactaac aattatagtt
     7921 aatcacgtaa accatgtcaa ttaatttact aataaaagat tattatttta gtaaatataa
     7981 ttatatgtgg aaaaagcagg taaattacct gcttttatat tttagctctg atgtttaaat
     8041 ttccttttca actcagctaa taaaactaat gctccgataa acattcctat atatccaaat
     8101 aattgaacct catctcccgt tgctactact gaactatcct tagactcatc aggttttacg
     8161 gttacttcac cagctttttt aaatgaggcc cttacacgaa tatcagccgt aacattttta
     8221 atcatatatg agttatcctt aatttcaact gccttttcat tgattagtaa ttgatcaag
//
LOCUS       DAOMTA010000023         8277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_92_length_8277_cov_2.501946, whole genome shotgun sequence.
ACCESSION   DAOMTA010000023 DAOMTA010000000
VERSION     DAOMTA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92_length_8277_cov_2.501946"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..284
                     /locus_tag="PLK68_01285"
     CDS             96..284
                     /locus_tag="PLK68_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89074.1"
                     /translation="MIRVYTAPGSQSCRKVIAWLKEHNLSFIEKNIFSSDLHANELRE
                     ILERCENGTDDILSKIVK"
     gene            320..457
                     /locus_tag="PLK68_01290"
     CDS             320..457
                     /locus_tag="PLK68_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRM89075.1"
                     /translation="MDELIAFIKANPSILKRPILMDEHRFQVGYNEEEIRTFIPRHQR
                     G"
     gene            698..1177
                     /locus_tag="PLK68_01295"
     CDS             698..1177
                     /locus_tag="PLK68_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM89076.1"
                     /translation="MLIRRAENRDITQINNLLKQVCLVHHKGRPDLFKKGARKYSDDQ
                     LMQIIKDDNRPIFVAVDNNEVVLGYVFCIFEQYLDSNILTDVKTLYIDDLCVDENSRG
                     QHIGKQLYEYSKDFAQKSGCYNLTLNVWSCNASAMKFYESCGLKPQKVHMETIFDKS"
     gene            complement(1284..1913)
                     /locus_tag="PLK68_01300"
     CDS             complement(1284..1913)
                     /locus_tag="PLK68_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM89077.1"
                     /translation="MKVILECSHQYINTIKRKLNAHLEQITYLDSFTGNDLSLFIKEV
                     KKLEDIENIKNIKSISNANIVCLIDSGLFMFELLEFNPLAFIRSANINADLDELINRI
                     EYLNKGLGVMLEFQCGYQTVRMNVKNITYVESYAHYLLIHTLNSTVKVREKISVALQK
                     LEPFGFIQVHRSYVINHDRIKKIDTDNITLSDETIIPIGKKYKQALKNH"
     gene            complement(1910..2539)
                     /locus_tag="PLK68_01305"
     CDS             complement(1910..2539)
                     /locus_tag="PLK68_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89078.1"
                     /translation="MKDKILIGEKMTKKGVFLNYLGLVFFGIIAVVGYNGLISKYLHL
                     NNQLNILIMIFIFIAVLLFLTPIIGSSEIIEFNHNEVRYFHTKGYFQQFAEVIRILTG
                     KQAVPDITLKTKDIEQVNLSYVPFLMMYAQQGYQIKITFLMNDGTTLAFLPSTIDQME
                     KGDYESAFKILETNGVEIVDKLMLRKALKMNKNDFYQYVNTIEKGRNKK"
     gene            complement(2654..4081)
                     /locus_tag="PLK68_01310"
     CDS             complement(2654..4081)
                     /locus_tag="PLK68_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRM89079.1"
                     /translation="MKQIKNINIETLENNLIYPIYEDQLIDPQLNKVLDYNLDKLVNK
                     KFKELTVIHTLGKYKFETITFIGLGSSKKITSKQIRDIAALIKLDEHATIYLEGINTD
                     HLDIHTITRAFIESYLQNSYQEHKIGHEPKVIHEFDIIFSQAELENDIQLGKVYGEGI
                     NYARMLSDTPSNLMTPDTLVDEAIKLSQKYPQLECTILNKSNLEKMEAGGILSVNQGS
                     NIPAYMICLKYNNSDDPYTAVIGKGLTFDSGGYNLKSDSYGMKYDMCGGADVLGVMQI
                     LAASQAKANVYGIIPTTENLVSDKAYKPQDVITTLSKKTVEIVSTDAEGRLILCDAIT
                     YVQQLGVKKIIDLATLTGACVSALGDVYTGVFSNCDEYYDEFVQALQISDEKGWRLPL
                     DSEYFEKLKSTSADFKNSAGKPGGGASVAANFLEAFIEEGTQWLHLDIAGSADNDGTG
                     ATGAMIRSVVNMINLKQSSKSERIK"
     gene            4242..4559
                     /locus_tag="PLK68_01315"
     CDS             4242..4559
                     /locus_tag="PLK68_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM89080.1"
                     /translation="MIKAGVDNMINNTEQETISIIQLTQQSSLAYQKVLAYVKDQNFV
                     KANEYLIKGNETLLEASKIHAGCLTVSSDLDLLLVHAEDMLISVQLYKSLIKEFIDIY
                     KKI"
     gene            4649..5932
                     /locus_tag="PLK68_01320"
     CDS             4649..5932
                     /locus_tag="PLK68_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89081.1"
                     /translation="MQKLMDKMENVLAPLATKIGSNKILKAISTAFNLIMPLIILGAI
                     FTLLSTLSLDAYQQFLADSGVGTVLSLVGKFTTDMLAVYVSFTAAYAFIRNEGMNTDA
                     IPAGLLSILAFFIMTPLANIVVEETTTSYIAFDYLGSKGLFTALIVGILVGYIYTFVV
                     KRGWIIKMPEGVPSTVAKSFNALIPGFVITTVFLIINGAFASLTGVTFSEWFYGIIAT
                     PLSALSGSLITYMVLTLLASVFWFFGIHGGQVTVPFSMMLFMQAGVENQAAFASGAPM
                     QNIITVGLLYFLMLGGIGNTLGLSIDMLLAKSNRYKSLGRLAILPSCCSINEPIVFGL
                     PLILNPIMALPYFLVPQINILITYFAMKSGLVSLPRIAMGATGTPVLLDGWLICGVSG
                     IILEIVLIIVSAILYYPFFKTQDNIALKEEAQIKE"
     gene            5998..7836
                     /locus_tag="PLK68_01325"
     CDS             5998..7836
                     /locus_tag="PLK68_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89082.1"
                     /translation="MDKNQKMIILELMNHETVSGSNLSSVIKMSTRSVRTIIKNINED
                     ICGAKIESGSFGYRLTIETPETFLAYLQRDQNGKEESRLAYLFNRFIDCNNYLKIDDL
                     CDELYLSRTQLKQSLKELREYLHDFDLTIATKAYYGMYLEGDEINKRRAIAHFEEYQM
                     DFDILQRIRDIVISSIANADYVISDDVLDNLVAHLYIAYYRVMKKEYANIDSEWLEEI
                     KEEKEYSLGCAIMELMNKIMAMEYRIEEVAYLTMHLCGKNSKQLSNNYINQEILDIVK
                     EMLMIIEKVANIPFQADLNLQLALSLHLIPLVKRIQYGTFMHNPLKDEIKSKLIMAYE
                     LAVKACVVINQRFNCTLSEDEIAYFALHINLSLEQKKYNFHRNNILVVCSSGVGSARL
                     LEYFFKENFNDYIEHLEVCSLHELENISLTKFDCIFTTVPLAIKVNIPIFLINNLINQ
                     RDTIKITNNLKQLNQANILDYFPEQLFFTYESFSSKEEAIHEIINECKKSYDLPADFE
                     QYVLQREALATTEFNDLIAFPHSNKPVSNATFVAVTILKKPLLWKKHKIRIILLSAIE
                     NKAIKELDDFYKIISNIISDSTIQWNLINNPNYQYFKEIIERLERL"
     gene            7833..>8277
                     /locus_tag="PLK68_01330"
     CDS             7833..>8277
                     /locus_tag="PLK68_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89083.1"
                     /translation="MKKILLVCGGGMSTSLLVKKMEAADIYNEYQIKCSDVVSGQVLL
                     VDYDIILLAPHIAYLQNEFVPLCQKVNIPLMLIDSYDYTKMAGKIVLDKAIMLLNEFT
                     KEHPFKVLLLHSVAGAMSDLIVLDMKKKVIGDEKDWQIESLTTEQF"
BASE COUNT         2868 a         1110 c         1356 g         2943 t
ORIGIN      
        1 aaaaaatctt taaaacaaat atgggaaaaa gaatttgtag gacaatgttt atgaagggct
       61 agtaatagtc cttattttat agatggagga ttattatgat tcgagtatat acagctccag
      121 gaagtcagtc atgtcgaaaa gttattgcct ggttaaaaga gcataatttg agttttattg
      181 aaaaaaatat tttttcgtct gatttgcatg ctaatgaatt acgtgaaatt ttagagcgct
      241 gtgaaaacgg aacagatgat attctttcta aaatagtaaa ataattaaat caaataaaat
      301 tgattttgat aatatgaaaa tggatgaatt aatcgccttc attaaagcca atccttcaat
      361 tttaaaaaga ccaattctaa tggatgagca tcgttttcaa gttggataca atgaagaaga
      421 aataagaaca ttcattcctc gtcatcaaag aggatagtta gtctagatat taaaatctag
      481 actttttact ttatgtaaaa agagcatggt tataattttt ttaaatattt atagaaactg
      541 attaagggag ataataagag aatttttgaa gaaattaatt tgaaagtatt ataatttagt
      601 aaaaaagtag tataattaat agttgataaa tgaaaataag tctataggta ttatgaagga
      661 taatattaag aattgagggt tatttaagga ggcagtcatg ttaattcgta gagcagaaaa
      721 tagagatatt actcagatca ataatttgtt gaaacaagtt tgtcttgtgc atcataaggg
      781 aagaccggat ttatttaaaa aaggggcaag aaaatatagt gatgatcagc ttatgcaaat
      841 aatcaaagat gataatagac caatcttcgt tgcagttgat aataatgaag ttgttttagg
      901 ctatgtattt tgtatttttg aacaatattt agatagcaat attttaacag atgtaaaaac
      961 attatatatt gatgatctat gtgttgatga aaattcaaga gggcaacata taggtaagca
     1021 gctttatgaa tattccaaag attttgctca aaagagcgga tgttataatt taacactcaa
     1081 tgtttggagc tgtaatgcat cagcgatgaa attttatgaa tcatgtggac ttaaaccaca
     1141 gaaagttcat atggaaacaa tttttgataa atcgtaatat gataattagt agtttttaag
     1201 aaaatgacat taattaagaa taaaacatcc tcctaataag gggatgtttt attctcaaag
     1261 agataactta caatatagga aaattaatga ttctttagag cctgcttata tttttttcca
     1321 ataggaatga tagtttcatc agataacgta atattatcag tatctatttt tttaatccgg
     1381 tcatgattaa tgacatatga tcgatgtact tggataaaac caaagggttc aagtttttgt
     1441 aaagccactg atattttttc tctaacctta accgttgaat ttaatgtgtg aattaaaaga
     1501 taatgagcat atgattcaac ataagtaata ttttttacat tcattctaac tgtttgataa
     1561 ccacattgaa attcaagcat tacccctagg cctttattga ggtactcaat ccggttgatt
     1621 aattcatcaa gatcggcatt aatattggca cttcgaataa aggcaagtgg gttaaattct
     1681 aaaagctcaa acataaataa accactgtca attagacaaa caatattagc gtttgaaata
     1741 cttttaatat ttttaatgtt ttcaatgtct tcaagttttt ttacttcttt aataaataat
     1801 gatagatcat tacccgtaaa tgaatctaaa taagttattt gctctaaatg tgcattaagt
     1861 tttcgcttta ttgtgttgat atattgatga gaacattcta aaataacttt cattttttat
     1921 tacgtccttt ttcaatcgta ttaacatatt gataaaaatc attcttattc atcttcaatg
     1981 cttttctaag cattaattta tcaacaattt ctacaccatt tgtttctaaa atcttaaatg
     2041 cagattcata atctcctttt tccatttgat caatagtact tggtaaaaat gctagagtag
     2101 ttccatcgtt cattaggaaa gttattttaa tttgataacc ttgttgagca tacatcatta
     2161 aaaatggtac atatgataaa ttaacttgtt caatgtcctt tgtttttaaa gtaatatcag
     2221 ggactgcttg tttacctgta agaattctaa taacttcagc aaattgttgg aaatatccct
     2281 tagtgtggaa gtagcggact tcattatgat tgaattcaat tatttcacta gagccgatga
     2341 ttggggttaa gaatagcaaa accgcaataa agataaatat catgattaga atattaagct
     2401 gattatttag atgaagatat ttagaaatta atccattata gccaactacc gcaataattc
     2461 caaaaaatac taaacctaaa tagttaagga acactccttt tttcgtcatt ttttcaccaa
     2521 ttaaaatttt gtctttcatt tttaattcct ccttacatct atcattatat gaataatcat
     2581 aagatagaaa atgagtatgg cacgaatgta tttttttatg gcattaatgt ttttaagaaa
     2641 aaaagtgaga gtattatttg atacgctcac tttttgacga ttgttttaag ttaatcatat
     2701 ttacaacact acgaatcatc gcaccagtag ctccggttcc atcattgtca gcactaccgg
     2761 cgatatcaag atgtaaccat tgtgttcctt cttcgataaa agcttctaaa aagttcgctg
     2821 caacactagc tccgcctcca ggtttaccag ctgagttttt gaaatcagca ctagttgatt
     2881 ttaatttttc aaaatattca gaatcaagcg gaaggcgcca acctttttca tcactaatct
     2941 gtaacgcttg gacaaattca tcatagtatt catcacaatt actgaaaaca ccggtatata
     3001 catcaccaag tgctgaaaca caagcgccgg taagggttgc aagatcaatg attttcttca
     3061 ctccaagttg ttggacatat gtaatagcat cacataaaat taatcgtccc tctgcatcag
     3121 tgctaacgat ttcgactgtt tttttagaga gggttgtaat aacatcttga ggtttgtaag
     3181 ctttgtcact aacaagattt tctgttgttg gaatgattcc atagacattt gctttggcct
     3241 gactagctgc taagatttgc attactccta aaacatcagc accaccacac atatcatatt
     3301 tcataccata actgtcactt ttaagattat aaccaccgga atcaaatgtt aagcctttac
     3361 caattacagc agtataagga tcgtcactat tattatattt taaacaaatc atataagcag
     3421 gaatattgct gccttgatta actgataaaa ttcctccagc ttccattttt tctaaattac
     3481 ttttatttaa gattgtgcat tctagttgtg gatatttttg actaagcttt atcgcttcat
     3541 caaccagggt atcaggtgtc attaaatttg atggtgtatc gctaagcatc cgcgcataat
     3601 taattccctc accataaact ttccctaatt ggatatcatt ttcaagttca gcctggctaa
     3661 atattatatc aaattcatga attacttttg gctcatgacc aattttatgt tcttgatatg
     3721 aattttgtaa ataactttcg ataaaagctc ttgtaattgt atgaatatca agatgatcag
     3781 tgtttatccc ttcaagataa atagtagcat gttcatctaa tttaattaaa gcagcaatat
     3841 ctcttatttg tttgcttgtt attttttttg aagaacctaa accaataaat gtaattgttt
     3901 caaatttata ttttcctaaa gtatgaatta cagttagttc tttaaacttc ttattaacaa
     3961 gtttatcaag attgtaatct agaactttat ttaattgtgg atcaattagt tgatcttcat
     4021 agattggata aattaaatta ttttctaaag tttcaatatt tatatttttt atttgtttca
     4081 tgtatgttac ctccgtactt ataagattat cattttttct taataaatga aaggtaaact
     4141 gcttgtttct tggtatcatt aaaataatta ctaaaagttc tttgtcaata tttccgccta
     4201 agtggaaata tcctgtcttt agatacaaat gaaggttttg tatgataaaa gcaggagttg
     4261 ataatatgat aaataatacc gagcaggaaa caatatcaat catacaatta acccagcaaa
     4321 gcagtctagc ttatcaaaag gtcttagcat atgtcaaaga tcaaaacttt gtcaaggcta
     4381 atgaatactt aattaagggt aacgaaacat tacttgaagc aagtaagatt catgctggat
     4441 gtttaacagt ttcaagtgat ttagatttat tattagtgca tgccgaagat atgttaattt
     4501 cagttcagtt atataaatca ctgattaaag aatttatcga tatttataag aaaatataat
     4561 tttcctacaa taacaggaaa ttaacagact tctagtttta ttagggtatt atataatgat
     4621 attgtaaata tgaaagggga tagttttaat gcaaaaatta atggacaaga tggagaatgt
     4681 tttagcacca ttagcaacaa agatagggag taataaaatt ttaaaagcaa tatcaacagc
     4741 ttttaattta ataatgccac taattatttt aggagcaata tttactctgc tgagtacttt
     4801 atcactagat gcatatcaac aatttttagc tgactcgggg gtcggaacag ttttaagttt
     4861 agtgggtaaa tttacaactg atatgttagc agtatatgta tcttttactg ctgcgtatgc
     4921 ctttataaga aatgaaggca tgaatacaga tgccatacct gccggtttgt tgtcaatttt
     4981 agctttcttt attatgacac cactagcaaa tattgtcgtt gaagaaacaa ctacaagtta
     5041 tattgctttt gactatttag gttcaaaggg attatttacg gccttgatcg taggaatctt
     5101 agttggctat atttatacgt ttgttgttaa acgtggctgg atcatcaaaa tgcccgaggg
     5161 cgtaccatca acagttgcca aatcgtttaa tgcattaatt cctggctttg ttataacgac
     5221 agtatttcta atcattaacg gagcttttgc ttcattaaca ggggtaacgt ttagtgaatg
     5281 gttctatggt attattgcga ctccattatc agcattatca ggtagcctaa ttacttatat
     5341 ggtcttaact ttgttagcgt ctgttttttg gttctttggt attcatggtg gtcaagtaac
     5401 ggtaccattc tcaatgatgc tatttatgca ggctggagtt gaaaatcaag cagcctttgc
     5461 atcaggggca ccaatgcaaa atattattac tgttgggttg ttatatttct taatgttagg
     5521 tggtattggt aatacgcttg gattatctat tgatatgtta cttgctaaaa gtaatcgcta
     5581 taaatcgtta ggacggttag caattttacc atcttgttgt agtatcaatg aaccaatagt
     5641 ttttggatta ccgctgattt taaatccaat tatggcttta ccatatttct tagtaccaca
     5701 aattaatatt ttaattactt attttgcaat gaagagtggt ctggtttcat taccaagaat
     5761 tgcaatggga gcgacaggaa caccggtatt attagatggc tggttaattt gcggagtatc
     5821 aggaattatt ttagaaattg ttttaattat tgtatctgca attttatatt atccattttt
     5881 taaaacacaa gataacatcg ctcttaaaga agaagcacag atcaaggaat aaaattaggc
     5941 tggatttcca gcctctttat catactttat gataaaatat aaacggaggt gcggatgatg
     6001 gataagaatc aaaagatgat aattttggaa ctaatgaatc atgaaactgt cagtggttca
     6061 aatttatcaa gcgtaattaa aatgagtact cgttcggttc gcactattat taagaatatt
     6121 aatgaagata tctgcggtgc taaaatagaa tcaggaagtt ttggttatcg tttaacaatt
     6181 gaaacaccag aaactttttt agcatatcta caaagagatc aaaatggtaa agaggaatca
     6241 cgattagctt atttgtttaa tcgatttatt gattgtaata attatcttaa aatcgatgat
     6301 ctatgtgatg aactttattt atctcgaaca caattaaaac aaagtttaaa agaattaaga
     6361 gaatatttac atgattttga tcttacaatt gcaactaaag catattatgg tatgtactta
     6421 gaaggtgatg aaattaataa acgacgtgca attgctcatt tcgaagagta tcaaatggac
     6481 tttgatattc ttcagcgaat tagagatatt gtaatttcat ctattgcaaa tgctgattat
     6541 gtaattagtg atgatgtatt agataaccta gttgcgcatt tatatattgc ttattatcgg
     6601 gtcatgaaaa aagaatatgc taatattgat tctgaatggc ttgaggaaat taaagaagag
     6661 aaagaatatt cattaggttg tgcaattatg gagttgatga ataaaataat ggcaatggag
     6721 tatcgaattg aagaggttgc ctatctaact atgcatcttt gtggtaaaaa cagtaaacaa
     6781 ctttctaata attatataaa tcaagaaatc ttagatattg taaaagaaat gttaatgata
     6841 attgaaaaag ttgctaatat tccatttcaa gcagatttaa atctccaatt agcattatct
     6901 ttgcatttga ttccattagt aaaacggatt cagtacggta cctttatgca taatccatta
     6961 aaggatgaga ttaaaagtaa gctgattatg gcttatgaat tagcggttaa agcttgtgtt
     7021 gtaatcaatc aacgttttaa ctgtacttta tcagaagatg aaatagctta ttttgcttta
     7081 catattaatt tgtctctaga acaaaaaaag tataattttc atcgtaataa tattttggtc
     7141 gtttgttcta gcggggtggg gagcgcgcgt ctattagaat atttctttaa agaaaatttc
     7201 aatgattata ttgaacatct tgaagtatgc tcattgcatg agctggaaaa cattagttta
     7261 actaagtttg attgtatctt tacgacagtt cctttagcaa ttaaagtaaa tattccaatc
     7321 tttttaatta ataatcttat taatcaaagg gatacaatta aaataacaaa taatttaaag
     7381 cagttaaatc aggctaatat actagattat tttccagaac agctcttttt tacatatgaa
     7441 tcatttagtt ccaaagaaga ggcaattcat gaaataatta atgagtgcaa aaaaagttat
     7501 gatctacctg cagattttga acagtatgtg cttcaaagag aggcgttggc aacaactgaa
     7561 ttcaatgatt taattgcttt tccgcattct aataaaccgg tttctaatgc tacttttgta
     7621 gctgttacga ttttaaagaa accattatta tggaagaaac ataaaattag aataatttta
     7681 ttatctgcaa ttgaaaataa agcaattaaa gaattagatg acttttataa aataatttcc
     7741 aatattatta gcgatagtac aattcagtgg aatttaatca ataatcctaa ttatcagtat
     7801 tttaaagaaa tcattgaaag gttagagagg ctatgaaaaa aattcttttg gtttgtggtg
     7861 gtgggatgtc gactagtctt ttggtaaaaa aaatggaagc agcagacatt tataacgaat
     7921 atcaaattaa atgtagtgat gttgtcagtg gccaagtttt gctggtagat tatgatatta
     7981 ttttattggc tccacatatt gcatatttac aaaatgaatt tgtgccgctc tgtcaaaaag
     8041 ttaatattcc attaatgctg attgatagtt atgattatac taagatggca ggtaaaattg
     8101 tgttagataa agcaattatg ttattaaatg agtttactaa agaacatccg tttaaagttt
     8161 tattattaca tagtgttgct ggagcaatgt ctgatttgat tgttctagat atgaagaaaa
     8221 aagtaattgg tgatgaaaag gattggcaga ttgaatcgct aactacagag cagtttg
//
LOCUS       DAOMTA010000024         8269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_93_length_8269_cov_2.223277, whole genome shotgun sequence.
ACCESSION   DAOMTA010000024 DAOMTA010000000
VERSION     DAOMTA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93_length_8269_cov_2.223277"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..293
                     /locus_tag="PLK68_01335"
     CDS             36..293
                     /locus_tag="PLK68_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRM89084.1"
                     /translation="MIENRCLNDLQMGEVGVITSLMAQGSIKRRLLDIGLVDDTEVQC
                     ILNSPSQDPKAFLIRGAVIALRNEDCQNVLIRKDDVNYGLN"
     gene            280..>1668
                     /locus_tag="PLK68_01340"
     CDS             280..>1668
                     /locus_tag="PLK68_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRM89085.1"
                     /translation="MGLTNKSTGINASETKLVIKKQSRNDKVVAIAGNPNVGKSTVFN
                     GLTGLHQHTGNWPGKTVTNAQGYCRGKENSYVLVDIPGTYSLLAHSAEEEVARNFICF
                     EHPDIIVVVCDATCLERNLNLVIQTIEIHTNVIVCVNLLDEAKKKGIDIDLEQLSSCL
                     GVPVIGITARKKTDLHKVIDSLDHAIDRPVNYKSLKIAYSDILLQAIRIVEEVLQKKV
                     TTSLSYQWLSLRLIENDPSLMIEMSNQLGLDYDDQELKEAIDQANELLAQHDINKDNL
                     QDKIVVSFIHSAHLICQKVVHYQKQDYNQRDRQIDKILTNRWTGYPIMILLLALIFWI
                     TITGANYPSQWLSDFLFGLEVPFNQFFHFIGTPLWLSDMLVSGVYRVLAWVVSVMLPP
                     MAIFFPLFTLMEDLGYLPRIAFNLDHAFKKCSACGKQALTMCMGFGCNAAGIVGCRII
                     DSPRERLIAMITN"
     assembly_gap    1669..1678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1679..2404
                     /locus_tag="PLK68_01345"
     CDS             <1679..2404
                     /locus_tag="PLK68_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM89086.1"
                     /translation="PTLIAILTMFFIGTEATMQNSLLSAIFLTMLIVLGITMTLIISK
                     LLSKTILKGVPSSFTLELPPYRKPQVGKVIVRSIFDRTLFVLSRAVAVAAPAGLIIWL
                     MANISVDGMTILNITSNFLDPFAKCIGLDGVILLAFILGFPANEIVIPIMIMAYLASG
                     SLIEMDNLVMLKSLLVENGWTWITAINVMLFSLMHWPCSTTLLTIKKEANSWKWAIVS
                     FLVPAVTGIIICSIFTMCANLFI"
     gene            2546..3166
                     /locus_tag="PLK68_01350"
     CDS             2546..3166
                     /locus_tag="PLK68_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HRM89087.1"
                     /translation="MTRPNFNDIQSFDEFKQYYWYRDELKQICKEHGITHTGTKTELN
                     DNIKAYFNGEIIKPVKRMKIRKQDVPLTLETKLLECGFVLNGRFREFFGEITKTPNFK
                     FTADMATALRRVRSEGNVHFTLKDLLDVYYGKSDYAKYDNSACQWNQFLKDFCRDENN
                     KIYYNKLKAASILWKVIRDQPGKKVYSASLLKKHQALLRQYKGENK"
     gene            3163..3576
                     /locus_tag="PLK68_01355"
     CDS             3163..3576
                     /locus_tag="PLK68_01355"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM89088.1"
                     /translation="MIREIVSDQFRLSQKSLPAAKEDLPVVQDLLDTIIAHATGCVGM
                     AANMIGINKNIIIVNDDGNYLVMLNPEIIKTMGRLYETEEGCLSHIGVKKTKRYEKIK
                     VAYYDVDFKKKIKTFSNYTAQIIQHEIDHCNGILI"
     gene            3643..3801
                     /locus_tag="PLK68_01360"
     CDS             3643..3801
                     /locus_tag="PLK68_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89089.1"
                     /translation="MIMRQNDDGRIIVIHSVVMKDISAYSVIGENSAHFRQECFGNEM
                     IELLGWLK"
     gene            3905..4360
                     /locus_tag="PLK68_01365"
     CDS             3905..4360
                     /locus_tag="PLK68_01365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM89090.1"
                     /translation="MEIRKYQSSDLKVICELFYETINTINRYDYNNDQIKVWSNRSNF
                     LLTQDDFFNSMYTLVAVENEKVLGYGNIDKRGYLDHLYVHKDYQGKQIATKLCDKLEQ
                     YCKDVKSLTVHASISAKPFFEKRGYKVIKEQSVKVDNVYLTNYLMEKTR"
     assembly_gap    4819..4828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4859..5056
                     /locus_tag="PLK68_01370"
     CDS             4859..5056
                     /locus_tag="PLK68_01370"
                     /inference="COORDINATES: protein motif:HMM:NF018425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRM89091.1"
                     /translation="MTEDFGIYYQQIIRIEKSRDAIASSANSLDRLNIVTEQKDFYIS
                     LQNNDLFISQIRKFKRELQYK"
     gene            5581..6255
                     /locus_tag="PLK68_01375"
     CDS             5581..6255
                     /locus_tag="PLK68_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRM89092.1"
                     /translation="MKRTKLDDRVLPEYTRGEEIFNMVTHIVGGVFGIAVLVLCIVFG
                     VLRHNNYGIASGIIYGISMILLYTMSSIYHGLSPNTKSKKVFQIMDHCSIFVLIAGTY
                     TPIVLCSIRPVDSFWAWMIFAVVWGCTVLGIILNAIDIESNEKFSMICYLAMGWCIIF
                     KFNLLPEAIGYNGIWLLVAGGVAYTIGTVFYGLQNKYRYMHSIWHLWILLGSVLHFLC
                     IILYVI"
     gene            7382..>7704
                     /locus_tag="PLK68_01380"
     CDS             7382..>7704
                     /locus_tag="PLK68_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM89093.1"
                     /translation="MKKEVGLLHLAVMLFGLAGVIGKFVTLPAILITFGRVSFSSIFL
                     LIIILIKKEKVRLSNKKDYILIIAAGIVMALHWFTFLQAIQLSTVAIGTITFSTFPLF
                     VTFLEP"
     assembly_gap    7705..7743
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7744..>8269
                     /locus_tag="PLK68_01385"
     CDS             <7744..>8269
                     /locus_tag="PLK68_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM89094.1"
                     /translation="IKNIMIALTMFAGVIITIPEFSLNNQITLGIFIGLIGSLSYAVL
                     CLMNRYFAGKYTGRTICLYEQGIAAIILLPSLLIVKASITKLDLFALIFLGVICTAVA
                     HSIYVSSLKKVKVQTAGIISGMESVYSIIFAIFLLHEIPGVKELLGGVIILGVAFYAT
                     VTNNKNIAKIMLYTL"
BASE COUNT         2851 a          933 c         1546 g         2880 t
ORIGIN      
        1 tccttaatct aatatacgat gaggagaaat ttattatgat agaaaataga tgtttaaatg
       61 atttacaaat gggtgaagta ggagtgatta cttcattaat ggcacaaggg agtattaaaa
      121 gaagactttt agatattggc ttagttgatg atacggaagt acaatgtatt ttgaatagtc
      181 caagtcaaga tcctaaagct tttttgatca ggggtgcagt aattgcttta cggaatgaag
      241 attgtcaaaa tgttttaatt agaaaagatg atgtgaatta tgggcttaac taacaaatct
      301 acaggaatta atgcaagtga gacaaagtta gtaataaaaa aacaaagtag aaatgataaa
      361 gttgttgcca ttgcaggtaa tcctaacgtt ggaaaaagca ctgttttcaa tgggctaaca
      421 ggccttcatc aacatactgg taactggcct ggtaaaacag ttactaatgc tcaaggctat
      481 tgtcgaggaa aagaaaattc atatgtttta gtagatattc ctgggacata ttcattatta
      541 gcacattcag cggaagaaga agtagcacgt aattttattt gctttgagca tccagatata
      601 attgttgttg tatgtgatgc aacttgttta gaacgtaact taaatttagt aattcagaca
      661 attgaaattc atacaaatgt aatagtttgt gttaatttgt tagatgaagc taagaaaaaa
      721 ggaattgata ttgatcttga gcaattatca tcttgcttgg gagttcctgt aattgggatt
      781 actgccagaa aaaagacaga tttgcataaa gttattgatt ctttagatca tgcaatcgat
      841 agaccagtta attacaaatc cttaaaaata gcttatagtg atattttatt acaagcaata
      901 agaattgttg aggaagtatt gcaaaagaaa gtaactactt ctttaagtta tcagtggctt
      961 agcttaagat tgattgaaaa cgatccttcc ttaatgattg aaatgtcaaa tcaattagga
     1021 ctggactacg atgatcaaga gcttaaagaa gctatcgacc aagcaaatga attattagca
     1081 caacatgata taaataagga taatttacaa gacaagattg ttgtttcatt tattcacagt
     1141 gctcatttga tttgtcaaaa agtggttcac tatcaaaagc aagattataa tcaaagggat
     1201 cgtcagatag acaagatttt aacaaatcgc tggacggggt atccaatcat gattttatta
     1261 cttgctttaa tattttggat aactataaca ggggcaaact atccatcgca gtggttatcg
     1321 gatttcttat ttggattaga agtacctttt aatcagttct ttcattttat tgggacgccg
     1381 ttatggctta gtgatatgct tgtatctggg gtatataggg tgttagcttg ggttgtaagt
     1441 gttatgctgc caccaatggc aatctttttt ccattgttta ctttgatgga agatttaggc
     1501 tatttaccaa gaattgcttt taatcttgat catgcattta aaaaatgcag cgcttgtggt
     1561 aaacaagctt taacgatgtg tatgggattt ggatgtaatg ctgctggaat agtaggatgt
     1621 cggattattg attcaccacg agagagatta attgcaatga ttactaatnn nnnnnnnncc
     1681 aaccttgata gcaatcttaa caatgttctt tattggaacc gaagcaacta tgcaaaattc
     1741 tctattatca gcaatatttt taacaatgtt aattgtttta ggaataacta tgactttgat
     1801 tatttcaaag ttattatcta aaactatatt aaagggagtc ccatcttcat ttactttaga
     1861 attaccacca tatcgtaaac ctcaagttgg caaagtgatt gttagatcaa tttttgaccg
     1921 aacattattt gttttatctc gagctgtggc agtagctgct cctgctggtc ttattatttg
     1981 gttaatggca aacattagtg ttgatggtat gacaatttta aatattactt ctaattttct
     2041 ggatcccttt gctaaatgca ttggtctaga tggcgttatt ttgttagcat ttattttagg
     2101 gtttcccgct aacgaaatag taattcctat catgattatg gcatacctcg ctagtggtag
     2161 tttgattgaa atggataatt tagtaatgtt aaaaagttta ttagtagaaa atggctggac
     2221 gtggatcact gcaattaatg taatgttatt ttcattaatg cactggcctt gttcgacaac
     2281 acttttaaca ataaaaaaag aagctaatag ttggaaatgg gcgattgtat ctttcttagt
     2341 tccagctgta accggaatca taatctgttc tatcttcact atgtgtgcta atctgtttat
     2401 ctaaatgatt tttaataata atgaaattga acctcttagt tggatttcca actaaggggt
     2461 gaactatata aagatgatat atggttttat tgagataagt agctatagat gatatgatat
     2521 taaaagacag gggtggagaa tataaatgac acgtccaaat tttaatgata ttcaaagttt
     2581 tgatgaattt aaacagtatt attggtatcg tgatgaacta aaacaaattt gtaaagaaca
     2641 cggaattact catacaggta ctaaaacgga actgaatgat aatattaaag cctattttaa
     2701 tggtgaaata attaaaccag taaaacgaat gaaaataagg aagcaagatg tgcctttaac
     2761 cttagaaaca aaattattag aatgtggctt tgttcttaat ggacgttttc gtgaattctt
     2821 tggcgaaatt acaaaaacac ctaattttaa gttcaccgca gatatggcaa ctgcattgag
     2881 aagggttcga agtgagggga atgttcattt cacgctcaag gatcttttag atgtgtatta
     2941 tggaaaaagt gattatgcta aatatgataa ttcagcttgt cagtggaatc agtttttaaa
     3001 agatttttgt cgtgatgaaa ataataaaat ttattataat aaattgaaag ctgcttcgat
     3061 attgtggaag gttattagag atcagccagg aaagaaagta tattcagctt cattattaaa
     3121 gaaacatcaa gctttgctaa gacaatataa aggggaaaat aaatgataag agaaatagtt
     3181 agtgatcaat ttagactaag tcaaaaatca cttccagctg ctaaagagga tttaccggtt
     3241 gttcaagatt tattagatac gattatagct catgctacag gttgtgtagg aatggcagct
     3301 aatatgattg gtatcaataa aaatattatc attgtaaacg atgatggtaa ttatttagta
     3361 atgttaaatc cagaaattat aaaaactatg ggacgtttat atgaaacaga agaaggatgt
     3421 ctatcacata ttggagtgaa aaagacaaag cgatacgaaa agataaaagt agcatattat
     3481 gatgttgatt tcaaaaagaa aataaaaact tttagtaatt atacagctca gatcattcaa
     3541 catgaaatag atcattgtaa tggaatttta atttaattaa agaagtaaaa ggaatgatat
     3601 aattgcagtt tactatataa atatttaatg gtaattttaa atatgattat gcgccaaaat
     3661 gatgatggaa ggattattgt aattcattca gttgtaatga aagatatttc agcatattct
     3721 gttattggag agaattcggc tcattttaga caagaatgtt ttggtaatga aatgatcgaa
     3781 ttgttgggat ggttaaaatg ataagattat gaataagaaa aactatgtga tctctccctt
     3841 tattacgtaa ggaagattta ggtgaagtaa aaaagagagt ataaaattat taatggtgaa
     3901 atcgatggaa attagaaaat atcagtcttc ggatttaaaa gtaatttgtg aattgtttta
     3961 tgaaacgatt aatacaataa atagatatga ttacaataat gatcaaatca aagtatggtc
     4021 aaatcggagt aattttctgc tgactcaaga tgactttttt aactctatgt atactcttgt
     4081 agctgttgaa aatgaaaagg ttttgggtta tggtaatatt gataagagag gttatttaga
     4141 tcatttatac gtacataaag attatcaagg aaaacagatt gcaacaaagc tatgtgataa
     4201 gttagaacag tattgtaaag atgtaaaaag tttaacagtt catgcttcca taagtgctaa
     4261 gccttttttt gaaaaaagag gatataaagt tattaaagaa cagtcagtca aagtggataa
     4321 tgtttattta actaattatt tgatggaaaa gactagataa tttgatttag cagtttaaga
     4381 tgccgttagg cattttttaa ttcgtgatag attattgatt atgagataat ataattagat
     4441 tgaggatatg gatagtgatt gtctttgttt tagaattaac taaggatggt aaagtaatca
     4501 agcaaggtaa ttttacttac aaaattagaa tatctttgtt ttgttatgat tagcaatata
     4561 tacttatatc gataaaggag taatttgaaa aatgagaatt tgagtcaaat ggtcttttta
     4621 agatatattt ttatgatgaa gaatcgttaa gtgatagaaa tgatgaatca gctaattagg
     4681 gtgttataat aaattaaatt gtttaaaggt gtctttatca ttaaaagtaa ccaggtgatg
     4741 cgattcaatt aacgatttaa ggtccttagc ctgagcaact ggctgtattt caatgctggc
     4801 gatttgtgca gcttcatgnn nnnnnnnnta ttttaatcag gattaaaagg aaagggggat
     4861 tactgaagat tttggaattt attatcaaca aattataaga atagagaaaa gtcgtgatgc
     4921 aattgcatca tcagctaatt ctttggatcg gctcaatatt gttacagagc aaaaagattt
     4981 ttatatttct ttacaaaata atgatttatt tattagtcaa attagaaaat ttaaaagaga
     5041 gttacagtat aaataatttt aataaagaaa ttaaaatagt agaaaatctt caaaacagtt
     5101 atatatttga ggacaagact gggatatgga ataaaaatag gggaactttt atattagtgc
     5161 ttatcataga aatttaaatg attttattga aaactatttg ttgaaggatg aaaataaatc
     5221 aaactgttta aacaaataca aaaagctggt tatcgctatt gtggaatcca gcatggtaat
     5281 tttaattatt atttattttg cggggacgga acacatatct tttagctgta tacattgtca
     5341 taataaagtt agaaaatatg attatttagt ttgatttgtt aaatatctaa atattttata
     5401 gcagtaagct aaaaatggat aattaaagat ttttagcaaa acaatatttt tgcaatcgac
     5461 aggcaaatac tgtgactaag tttggtttta tcaaagaaat ttataagaag ttattatatg
     5521 atgttcttga aattaacatg aaatagggct atattcatac atatagattg ggggtattca
     5581 atgaaacgaa caaaattaga tgatcgagta ttacccgagt atactcgagg tgaagaaatt
     5641 tttaatatgg ttactcatat tgtaggtgga gtttttggaa ttgccgtatt agtactttgt
     5701 attgtttttg gagtattgcg ccataacaat tatggtatag cttcaggaat tatttatgga
     5761 atatcaatga tcttgctata tacaatgagt agtatttatc atgggctttc cccaaatact
     5821 aagagtaaaa aagtgtttca aattatggat cactgttcaa tttttgtatt gattgctggt
     5881 acttatacac ctattgtgtt gtgttcgata agaccagtag attcattttg ggcttggatg
     5941 atctttgctg ttgtttgggg ctgtacggtt ctaggaatca ttttaaatgc aattgatatt
     6001 gaaagtaatg aaaagttttc aatgatttgt tatttagcga tgggctggtg tattatcttt
     6061 aaattcaatc tattgccgga agctattgga tataatggaa tttggttact tgtggctggt
     6121 ggtgtagctt atacgattgg taccgttttt tatggtttgc aaaataagta tcgctatatg
     6181 catagtattt ggcatttatg gatcttatta ggaagtgtgt tacatttctt atgtataatt
     6241 ttatatgtta tttaaaaggt atcattttaa aaatgatgct ttttttacga taatatagag
     6301 aaaatggggc taaagagtat cataatagtg tggtattaat aattcaatga ggagatgata
     6361 ggtataaatc tattttatcc atcaattatt aagtttgctc gttttaaaat gcaaccaaaa
     6421 agtaatgata tttttactat tctcgaaaat gtccactaag tagttgatat tccttatcaa
     6481 gatgacagga atcataatca tttactagat atttattatc cttaatccac gactaagcca
     6541 ttgtctatga tcattgatgt tcacggtgat gcctggattt atggtgataa aggagtaaat
     6601 aaaaatttgc agttatgcta gctgctaaaa attttactgt tgtgaatata aattatactc
     6661 taataccaca agcctcgttg aagcagtaag ttcaggaaat tattatgact ttaaagtttg
     6721 ttgctaaaac aaagaataaa tatttttgga atattgatca aattattttt actgagtgat
     6781 tcagctggtg ggcatttagc aggattggtt tatgtaatca taaaacagcc tgagctctgt
     6841 aaagtatata atgttcagcc accagaaatt atgattagag agttttgtta catggtggag
     6901 atgatttaat aagttttgac taacagtaaa aattttgttt atcagtaggt tagtgttatg
     6961 gttcagggag ttaacaatga agattcagac atatatcgaa aagcttgcct tattgatgta
     7021 attgatagtt accgccactt tcaacacgta ttattagtag tgagataact tacattttta
     7081 gtcaataaag gctaaaaatg tattgaaggc ttataactgg tatgtttgaa ccgtgtgttg
     7141 ctctaaggaa aacatttagg acatgttttt gaagttctgt atcctgaata tcttgaaact
     7201 cagtcaacga ttgaacgaat taaatatttt attaataact aattataatg aaggttttta
     7261 agtagttatt ggccttgcat tttagtttag aaaatggaca gcgatgatat aggtggttta
     7321 tctttttttc ttgttaaaaa tgagctaaac tattattaac tataaatagg aggggaaagt
     7381 gatgaaaaaa gaagttggat tattacattt ggctgttatg ttatttggct tagccggagt
     7441 aattggaaaa tttgtcacac taccagctat tttgattacc tttggacgag tatctttttc
     7501 ttcaattttt ctattaataa taattttaat aaaaaaagag aaagttagat tgagtaataa
     7561 aaaagattat attctaatca tcgctgctgg tattgttatg gcacttcact ggttcacttt
     7621 tttacaagca attcaattat caactgttgc aattggaaca attacttttt caacttttcc
     7681 actttttgtg acttttttag aaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnataaaaa atataatgat tgcgctaaca atgtttgctg gggtaataat tacaattcca
     7801 gagttctcac ttaacaatca aataacactg ggaatcttta ttggattaat cggatcttta
     7861 agttatgcgg tgttatgctt gatgaatcgc tattttgcag gtaaatatac agggagaacg
     7921 atttgcttat atgaacaagg aattgctgcg attatattat taccgtcatt gctgatagtt
     7981 aaagcttcaa taacaaaact tgatttattt gcgcttattt ttctaggagt tatatgtacg
     8041 gctgttgctc atagtattta tgtcagtagt ttaaagaaag taaaggtgca aactgcaggg
     8101 ataatatcgg gaatggagag tgtgtatagc attatttttg cgatattttt gttacatgag
     8161 attcctggag taaaggaact gcttggagga gtgattattt tgggggtggc tttttatgcc
     8221 actgttacta ataataaaaa tatcgcaaaa ataatgctat acacactct
//
LOCUS       DAOMTA010000025         8212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_97_length_8212_cov_2.430060, whole genome shotgun sequence.
ACCESSION   DAOMTA010000025 DAOMTA010000000
VERSION     DAOMTA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97_length_8212_cov_2.430060"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1131
                     /gene="vanG"
                     /locus_tag="PLK68_01390"
     CDS             82..1131
                     /gene="vanG"
                     /locus_tag="PLK68_01390"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HRM89095.1"
                     /translation="MKRKTIAILFGGKSSEYEVSLQSAAAIIKNFPQEKYDLYLIGIT
                     RAGQWFHYDGEIEKISNDTWNQELLNPVTVCLEPERQAVMEIVDNKIIYQQIDAVLPV
                     LHGKNGEDGTVQGLMELAKIPVLGCGLLASALCMDKYRAHQLVKAAGIKVTRSVLVHN
                     INDFNVKALNFPLFVKPLKAGSSYGISKVVKMEELTKALEYAFNYDNEVIVEEAVEGF
                     EVGCAIIGNDELTVGEVDEIELSGGFFDYDEKYTLKTSTIHMPARIDEVTARKIQETA
                     KIIYQTLACQNFARVDMFLTPNNEIVFNEVNTIPGFTDHSRFPQMMKGVGFEFSELID
                     KIVAIGLKYADGNFK"
     gene            1109..1861
                     /locus_tag="PLK68_01395"
     CDS             1109..1861
                     /locus_tag="PLK68_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM89096.1"
                     /translation="MQMVTLNKDDIYQGPLVLVNHNYPIANNYQITNCSTAKDSTISL
                     EKTAMEMLTKALQEIKAEDKIVLVSGLRSGQEQAMIYNQAIQNHGKEFVDKFVARVNC
                     SEHQTGLAIDLAVKNETIDIFCPDFTSQEICQQFRDIASDYGFIQRYSKNKIDFTKIS
                     EEPWHFRYVGIVHAKIMEENQWCFEEYHLYIKNHSIFNPYVLDCQGKLVEVFYVPAVN
                     EQTKLNLSDHGSYQISGNNCDGFIITSWRPYV"
     gene            1854..2888
                     /locus_tag="PLK68_01400"
     CDS             1854..2888
                     /locus_tag="PLK68_01400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRM89097.1"
                     /translation="MSKVKGYPLIDCFRIIAAILVVTIHTSPLDTISPTLDFYLTRGL
                     GRIAVPFFFITTAYFYFLNPSKKRLYKIIKQTLLIYLAAIIIYLPLNLYNHYFNQSNL
                     IYEIIKDVLFDGTFYHLWYLPATVLGLIIVAVLDKRVSLLTSFSIVVLLYLIGLGGDS
                     YYGLIHPYCKWGYDLIFLISDYTRNGIFFAPLFIWIGKLLAVKQFNFSLRVIVISTIL
                     AISLMFIEISLLKGYQDAADYVFVRHDSMNILLPVVMFWLYQGLLRISGKRISKCKDL
                     SLAIYLFHPLMIVVVRGIGKLLKCDHLVVDPLIQFISVLIITVLFSEILLKGKEKYGE
                     YSHKSQLDRS"
     gene            2848..3978
                     /gene="vanT"
                     /locus_tag="PLK68_01405"
     CDS             2848..3978
                     /gene="vanT"
                     /locus_tag="PLK68_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HRM89098.1"
                     /translation="MVNIRTSRSWIEVDLDAITHNLDELSSLLNKNSSLMVVVKAEGY
                     GHGLVEVAKHCQKMGIEAFAVATIDEGIKLREHGIYGEILVLGYSHPIRAIELHNYRL
                     IQTIVNREHARQLNESGHMINVHIKIDTGMHRLGFDLSDYESIKEIYDYQFIHIQGYF
                     THLCVCDSKRKVDQEYTQKQIAAFYDLINELKLRHYDIGKVHLQSSYGLVNYPEINAD
                     YARVGILMYGVYSTFNDYSKVQLDLKPALTIKAQIAMLHYLTKGASLGYGLTYKVNRD
                     SVIAVIPIGYGDGLPRILSSNGSVLIKGQKCPIVGRICMDQMLVDVSEISDISDNDLV
                     TVIGQDGTEEIRVEEIALEANTISNEILSRLGSRLPRIYRGG"
     gene            3983..5191
                     /locus_tag="PLK68_01410"
     CDS             3983..5191
                     /locus_tag="PLK68_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM89099.1"
                     /translation="MKRSSYMSYNEKKKIPYKVIISVLIAMLILGTLIAFNFTRIRLM
                     TKGYGWSEQSIILQLNDNEIERYLDAEKVSDLSSWNEYKNKKHYLEYQTYQKEHQDYS
                     KKEVIKYIDTFYQDYYQDLKDLKYSYKQVLSLMKVAELSDFKVIVDNKYTYSQIKSYL
                     KINGMVFEDLPKYLASNQEPITAVLTVTYPFIDANNAVGSEYEVLDPSNTLLLIKKGF
                     VLPKDYVPADLVVPDIPIAPDNNHNKLRKDAAKALEDMNKDALKEDYHLVLNSGYRSY
                     DEQVEIYNDYFNRYDEVTASGLVAKPGSSEHQLGLGVDLTSQSVIDKKRMVFGDTDEY
                     KWVAKNAYKYGFILRYPKNRSDITGTANEPWHLRYVGKKAAKIIYDNNWTLEEYILKY
                     GFDYELKKID"
     gene            5271..5732
                     /locus_tag="PLK68_01415"
     CDS             5271..5732
                     /locus_tag="PLK68_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRM89100.1"
                     /translation="MDIYKFLTQLGIDYQKINHQSVSTVEEAMFINEQIDGVACKNLF
                     LKSSDKQFFIYMMTVERRADLKYIAKQIGSKRLSFASDEELVELLKLIPGSVTPLGII
                     NDNKRVKILIDRELRAQRLLIHPNINTATISITYDDLLLFINACGNEYLII"
     gene            5904..7733
                     /locus_tag="PLK68_01420"
     CDS             5904..7733
                     /locus_tag="PLK68_01420"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HRM89101.1"
                     /translation="MLVTDKELHRIVSSVRNLRTGRIYYQKDYVREITITYDRLFDDY
                     TIEGIVDNGSYENVCKIVIDQDNKITDYSCNCYWCDELSACGHIGAVLLKVQELSPDD
                     FPFKYKSKNGKISYFDKLVQIRQQQEEELLKNELAVTKNLIASFKTAKAIDFNQVKLN
                     QKYDLEVQIKINYGHPCLKFKVGSDKKYIIKNIPNFLSALANNEYVEYGKQLAFVHNQ
                     KVFSDEALKIIELMKFCMITYERYQQEYYYDISHVTNEIKIIPESIEYIYEVLKDLEK
                     TNQHLSIYQYNYQPTLHIRKLEKYYSLELKDFDEFCGYKNLYKITENEIGMVKLDDEG
                     KAIKFIHNCESKDKLLLAKEDVNDFVKYILNDIKQYFNYTGDLLTSEVIETEILTLYG
                     DINELEQIKVYLESKQGLETIYSFQQPRKQTSINFDLIEDYLKEIGDVVDFEKHCMYL
                     NLESEKTYSFLNEGLPFLANYCEVMVSDALKKIGKKSQFSINVGVTLENDLLTIDIES
                     IDIPKDELALVLNSYQKKKKFHRLKNGQLLYLDSDELEELNEFMTDYQIRPKMLEDGH
                     LEMDVYRAFSLDNKAETSNYLVYDRSTVFKEIIDNFKDIAKQS"
     gene            7803..>8212
                     /locus_tag="PLK68_01425"
     CDS             7803..>8212
                     /locus_tag="PLK68_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRM89102.1"
                     /translation="MSSYGFGGILADDMGLGKTLQMIVLLDQNRDDKKTSLVVCPSSL
                     LLNWQDEIHKFSNSLSCTCIHGSLKQRKEAIRKFNEVDVLITTYDYMRRDANLYDGYQ
                     FENIILDEAQYIKNPKTKNAITVKSLKAKHRFALT"
BASE COUNT         2962 a          935 c         1534 g         2781 t
ORIGIN      
        1 ttcttatgaa gaaattaaaa aatgaagagc gtttttttta tattatagat ttatcgaagg
       61 agtagtaagg aaggatataa tatgaaaaga aaaacaatag ctattttatt tggaggaaaa
      121 tcaagtgaat atgaagtgtc gctacaatca gcagctgcga ttataaagaa ttttccgcaa
      181 gaaaaatatg atctttattt aatcgggatt acgagagcag gacagtggtt tcattatgat
      241 ggggaaattg aaaaaattag taatgatact tggaatcaag aactgcttaa tccagtgacg
      301 gtatgtttag aaccagaaag acaagcagtg atggaaatag ttgataataa aataatctat
      361 cagcaaattg atgcggtttt accggtcttg catggaaaaa atggtgagga tggtactgtt
      421 caaggattaa tggagcttgc aaaaatacca gtacttggct gtggattatt agcatcagca
      481 ttatgtatgg ataaatatcg agctcaccag ttagtcaaag cagcagggat aaaggttact
      541 aggtctgtgc ttgtacataa tataaatgat tttaatgtta aagcattgaa ttttccatta
      601 tttgttaaac cattaaaagc tggatcatca tatggaatta gtaaagttgt caaaatggaa
      661 gaattaacaa aagcacttga atatgccttt aattacgata atgaagtaat tgtagaggaa
      721 gctgttgaag ggtttgaggt aggttgtgcg attattggca acgatgaatt gactgttggt
      781 gaggtagatg aaatagaatt atcgggaggt ttttttgatt atgatgaaaa gtatactttg
      841 aaaacttcaa caattcatat gcccgctaga attgatgaag taacggcaag gaaaattcaa
      901 gaaactgcaa aaattattta tcagacatta gcttgtcaga attttgctag ggtagacatg
      961 tttttaacgc caaacaatga aatagttttt aatgaggtta atacgattcc tggttttact
     1021 gatcatagcc gttttcccca gatgatgaaa ggagtaggtt ttgaatttag tgaattaata
     1081 gataaaattg ttgcgatagg gcttaagtat gcagatggta actttaaata aagatgatat
     1141 ttaccaaggt ccattagtat tggttaatca taattatcca attgcaaata actatcaaat
     1201 tactaactgt tcgactgcca aggattcaac aatatcatta gaaaaaacgg ctatggagat
     1261 gctaacaaaa gcattacagg aaattaaagc agaagataag attgttttag tcagtggttt
     1321 aagaagcggt caagaacaag ctatgatcta taatcaggct attcaaaatc atggaaaaga
     1381 gtttgttgat aagtttgttg ctagagtgaa ctgtagtgag catcaaacag gtctagccat
     1441 tgatctagca gtaaaaaatg aaacaattga tatcttttgt cctgatttta caagtcagga
     1501 aatctgtcaa caatttcgtg atattgcaag tgattatggc tttattcaga gatattctaa
     1561 aaataaaatt gattttacca aaattagtga ggaaccatgg cattttcgct atgttgggat
     1621 tgttcatgca aaaattatgg aagaaaatca gtggtgtttt gaagaatatc atttatatat
     1681 aaaaaatcat tctattttca atccgtatgt tttagactgc caaggaaagt tagttgaagt
     1741 attttatgtt ccagcagtta atgaacagac taagttaaac ttaagtgatc atggtagtta
     1801 tcaaatatcg ggaaataatt gtgatggttt tattattaca tcatggaggc catatgtcta
     1861 aagtaaaagg ttatccctta atcgactgtt ttaggataat tgctgcaatt ttagttgtca
     1921 cgattcatac ttcaccatta gatacaatta gtccaacttt agatttttat ttaactcgtg
     1981 gattaggacg tattgcagta ccattctttt ttatcacaac tgcttatttc tattttctta
     2041 atcccagtaa aaaacgacta tataagatta ttaaacagac actattgatt tatttagcag
     2101 caataattat ttatttacca ttaaatcttt ataatcatta ttttaatcaa agtaatctga
     2161 tttatgaaat aattaaagat gttttgttcg atggaacttt ctatcatttg tggtatttac
     2221 cagcaacagt tctaggattg atcattgttg cagtattaga taaacgggta tctttattaa
     2281 cgagtttttc aattgtggta ctactttatt taattggatt aggtggtgat tcgtattatg
     2341 gtttgataca cccatattgt aaatgggggt atgacttgat ttttttaata agtgattata
     2401 cacgaaatgg tattttcttt gcaccgttat tcatttggat tggaaaactt cttgcggtaa
     2461 agcaatttaa ctttagttta agagtaatcg ttattagcac gatattagca ataagtttga
     2521 tgtttataga aatatcacta ttgaaaggtt atcaagatgc agcagattat gtttttgtac
     2581 gtcatgattc aatgaatatc ttattgccgg tagtaatgtt ttggctatat cagggacttt
     2641 tacgaatatc aggcaaacga atatctaaat gtaaagatct atcgttagct atttatcttt
     2701 ttcatccctt gatgattgtt gttgttcgtg gaataggtaa attattgaag tgtgatcatt
     2761 tagttgttga cccgctgatt cagtttatta gtgtattgat aataactgtt ttattttcag
     2821 agattttatt gaaaggaaaa gagaagtatg gtgaatattc gcacaagtcg cagttggatc
     2881 gaagttgatc tagatgcaat cacgcataat cttgatgaat tgagttcatt attaaataaa
     2941 aatagttcat taatggtagt tgttaaagca gaaggatatg gtcatgggtt agtagaagtt
     3001 gcgaaacatt gtcaaaaaat gggaattgaa gcttttgcag ttgctactat cgatgaaggt
     3061 atcaaacttc gagaacatgg gatatatggc gaaattttag tacttggtta tagtcatccc
     3121 attcgtgcaa ttgaattaca taattatcgt ttgattcaaa ccattgtaaa tcgtgagcat
     3181 gctcggcaat taaatgaaag tgggcatatg atcaatgttc atattaagat tgatactgga
     3241 atgcatcgtt taggttttga tttaagcgat tatgagagta ttaaagaaat atatgattac
     3301 cagtttattc atattcaggg ttattttaca catctttgtg tttgtgatag taaaaggaaa
     3361 gttgatcaag aatacaccca aaaacaaata gctgcttttt atgatttaat taatgaatta
     3421 aaattaagac attatgatat aggaaaggtt catcttcaaa gtagttatgg attagtaaat
     3481 tatcctgaaa tcaatgccga ttatgctcgg gttgggattt taatgtatgg agtgtactca
     3541 acgttcaatg attattcaaa agtacaatta gatttaaaac cagctttaac aattaaagca
     3601 caaattgcaa tgctgcatta tttgactaaa ggcgcgagtc ttggttatgg cttgacttat
     3661 aaggttaatc gtgatagtgt aattgccgtt attccaattg gctatggtga tggattgcca
     3721 cgtatacttt cttcaaatgg aagtgttttg atcaaaggtc aaaagtgtcc aatcgttgga
     3781 agaatttgta tggatcaaat gcttgtagat gttagcgaga ttagtgatat cagtgataat
     3841 gatctagtca ccgtaattgg tcaagatggt acagaagaaa ttagggtaga agaaattgca
     3901 cttgaagcta atacaattag taatgagatt ttgagtcgtt taggaagtcg actgccaaga
     3961 atatatcgag gaggataata ggttgaaaag aagcagttac atgtcgtaca atgaaaagaa
     4021 aaaaatccct tataaagtaa tcattagtgt attaatcgct atgttaatac ttgggacatt
     4081 gattgcattt aattttacaa gaattcggtt aatgacgaag ggctatggtt ggtctgaaca
     4141 aagcattatt ttacagctaa atgataatga gattgagcgt tatttggatg cggaaaaggt
     4201 cagtgattta agttcctgga atgaatataa aaataaaaaa cattatttag agtatcagac
     4261 atatcaaaaa gaacatcaag attattcaaa aaaagaagta ataaaatata ttgatacttt
     4321 ctatcaggat tattatcaag atctcaagga tttaaaatat agttataaac aagtattatc
     4381 tttaatgaaa gttgcagagc tgtctgattt taaggtaatc gtcgataata aatatacata
     4441 ttcgcaaatt aaatcttatt taaagattaa tggaatggtc tttgaggatc tgcctaagta
     4501 tcttgcaagt aatcaagagc caataacagc tgtacttacg gttacttacc catttattga
     4561 tgctaataat gctgttggat cagaatatga agttttagat cctagtaata cacttctgtt
     4621 gattaaaaag ggttttgtgt tacctaaaga ttatgtccct gctgatcttg ttgtacctga
     4681 tataccaatt gctcctgata ataaccataa taaacttcga aaagatgcag ctaaagcatt
     4741 agaagatatg aataaagatg ctttaaaaga agattatcat ttagttttaa atagtggata
     4801 tcgctcatat gatgaacaag tagaaattta taatgattat tttaaccgtt atgatgaagt
     4861 cactgctagt ggtttagttg cgaaacctgg ctcaagtgaa catcaattag ggttaggagt
     4921 agatttaact agccaaagtg taattgataa aaagagaatg gtttttggtg atacggatga
     4981 gtataaatgg gttgctaaaa atgcttataa atatggcttt attcttcgtt atcctaaaaa
     5041 tcgttcggat attactggaa ctgctaatga gccatggcat ttacgttatg ttggtaagaa
     5101 agcagctaaa ataatttatg ataataattg gactctagaa gaatatattt taaaatatgg
     5161 ttttgattat gaattaaaga aaattgatta agcatccttt ggatgctttt attattttca
     5221 attaaacttt taatatgtta aaatcattat cataaaaaga ggagttaaca atggatatat
     5281 ataaattttt aacacagtta ggtattgatt atcaaaaaat taatcaccaa tcagttagca
     5341 ctgttgaaga agcaatgttt attaatgagc aaattgatgg agtagcatgt aaaaatctat
     5401 ttttaaaaag tagtgataaa caattcttca tttatatgat gacggttgaa aggcgtgctg
     5461 acttgaaata tatagctaaa caaattggta gtaagcggct aagctttgct agtgatgaag
     5521 aacttgtaga attgttaaag ttaattccag gatcagtgac accattagga ataattaatg
     5581 ataataagcg agtaaaaata ttgattgatc gtgaattaag agcacaacga ttattaattc
     5641 accctaatat taatacagca acaatatcaa ttacctatga tgatttgtta ttgtttatta
     5701 atgcttgtgg aaatgagtat ttaattattt aaaaatttga ttatggcagt aataagaaat
     5761 ttattgaaat aaatacttgc tgcaagatta catctttata actctatgta tttagtttag
     5821 tcataatggt tagagtcgta atattaatga atattattcg atatatgcta taattttaaa
     5881 gaaaattagg aggtgactgg taagtgcttg taactgacaa agaattacat agaatcgtaa
     5941 gcagtgttag aaatctaaga acaggacgaa tatattatca aaaagattat gttagagaaa
     6001 tcacaattac ttatgatcga ctttttgatg attatacaat tgaaggcatt gttgataatg
     6061 gtagttatga aaatgtgtgt aaaatcgtta ttgatcaaga taataagatt actgactata
     6121 gttgtaattg ttattggtgt gatgaactct cggcttgcgg tcatattggg gctgttttgc
     6181 tgaaggttca agagttatca cctgatgatt ttccatttaa atataaaagt aaaaatggta
     6241 agataagtta tttcgacaag ttagtacaaa ttcgtcagca gcaggaagaa gaactgctaa
     6301 aaaatgaatt agcagtaaca aaaaacctaa tagcatcatt taagactgct aaagcaattg
     6361 attttaatca ggttaaacta aatcaaaagt atgatcttga agtccagatc aagattaatt
     6421 atgggcaccc atgtttaaaa ttcaaagtag gaagtgataa aaagtatata ataaaaaata
     6481 ttcctaattt tttaagtgca ttagcaaata atgaatatgt tgaatatggt aaacaattag
     6541 cgtttgttca taatcaaaaa gtatttagtg atgaagcact gaagattatt gaattaatga
     6601 aattttgtat gatcacttat gagcggtatc aacaggaata ttattacgat atatcgcatg
     6661 tgacaaatga gataaagatt attcccgaaa gtattgaata tatctatgaa gtattaaaag
     6721 atttagaaaa aactaatcaa catttatcaa tttatcaata caattatcag cctactttac
     6781 atataaggaa acttgaaaaa tattattcgc ttgaattaaa ggattttgat gaattttgtg
     6841 gttataaaaa tctttataag attactgaaa atgaaatagg tatggttaag ctggatgatg
     6901 aaggaaaagc aatcaaattt attcataatt gcgaaagtaa agacaaatta ttattagcaa
     6961 aagaagatgt aaatgacttt gttaaatata ttttaaatga tattaagcaa tattttaatt
     7021 atactgggga cttattaaca agtgaagtta tcgaaacaga aatattgaca ctctatggcg
     7081 atattaatga attggaacaa atcaaagttt atcttgaaag taaacaaggc ctagaaacga
     7141 tttattcatt tcaacagccg agaaagcaaa catcaattaa ttttgattta attgaagatt
     7201 atttaaaaga gattggtgat gtagttgatt ttgaaaaaca ttgtatgtat ttgaatttag
     7261 aaagtgaaaa aacttactcc tttttaaatg aaggtttgcc ttttttagct aattattgtg
     7321 aagtgatggt tagtgatgca cttaaaaaga ttgggaagaa aagtcaattt agtattaatg
     7381 taggagtgac tttagaaaat gacttattga caattgacat tgaaagtatt gatattccta
     7441 aagatgaatt agcgttagtc ttaaactcat atcaaaagaa gaagaaattt catcggttaa
     7501 aaaatggtca actattatat cttgattctg atgagctgga agaattaaat gaatttatga
     7561 ctgattatca aattagacca aagatgcttg aggatggaca tttagaaatg gatgtctatc
     7621 gggcttttag tcttgataat aaagccgaaa caagcaatta tttagtatat gatcgtagta
     7681 ccgtatttaa agaaattatt gataatttta aagatatagc taaacagagt tagcccttaa
     7741 cgcctaatta tcaagaaatt ttacgcgatt atcagaaatt tggatatcaa tggcttcaat
     7801 caattagttc ttatggcttt ggaggaattt tggccgatga tatggggctt ggtaaaacct
     7861 tacaaatgat tgttttactg gatcaaaatc gggatgataa aaaaactagt ttagttgttt
     7921 gtccatcatc attgttattg aattggcaag atgagataca taaattttct aattctttat
     7981 cttgtacttg tattcacggc agtctaaaac aacgaaaaga agcaattaga aaatttaatg
     8041 aggttgatgt gctaattact acttatgatt atatgcgtcg tgatgctaat ctttatgatg
     8101 gttatcaatt tgaaaatatt attttggatg aagcgcaata tattaaaaat cctaaaacta
     8161 agaatgcgat aacagtaaaa tcgttaaaag ctaaacatcg ttttgcttta ac
//
LOCUS       DAOMTA010000026         8154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_100_length_8154_cov_2.263736, whole genome shotgun sequence.
ACCESSION   DAOMTA010000026 DAOMTA010000000
VERSION     DAOMTA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_8154_cov_2.263736"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2535)
                     /locus_tag="PLK68_01430"
     CDS             complement(<1..2535)
                     /locus_tag="PLK68_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRM89103.1"
                     /translation="MYIKDDEINRIFNEKNWKIAHSYYKDNVITNMSVLKQGNEYHID
                     GTVEIYGRSSTCHIVVDTGGKINNFECDCPYCHNDELACGHIGVLLLKFYSLEMSEIP
                     FQFNQKIDYKAKIEAFERVREEKLIQTKLEESKKLITDYTNQQDPQLTNDSLIDLIPN
                     INYSFNRILISYRIGQNKTYLIKNLKEFIYNYRYHNVYTYGSQLTTDYNRKSFSSEAL
                     KQIDFIRDNSEFIDDIERYRSIDINKYNIDEFFETYLTNLNINMFFTTIDLNNLTINI
                     EDKEDYFEISLLPLGGQLIIGNQYLYYLDKNILNRYSLKMSKITKKLISKLDKENLIV
                     AKEDFGYFGKYIIDQILPYIAITGANIDDYMPSVITLLTYVDLNNFGDLTINLEYRDD
                     EGNILFDGKYIDSYETKLPLNVDTALAMIEDYAQYDELTNMYLITNNDEDIYYFIKNI
                     LPKLNRYCDVFVSEDIKNINKPKNISLNIGIRLKNDLLEIDLDSINVSKDEIRDILES
                     YQKHKAYHRLKNGEFVNLEDQTLNEAYNLIQDLNLENKNIQDGAIIVDKSKALFLNEL
                     IQDSETINFNRNQQFQELINHLTNCNINNYPVPEPFSDILRDYQCTGYKWIKTMSDYG
                     FGGILADDMGLGKTLQMITVLEDAKKNHKASIVITPATLILNWQDEIKKFSNDLNVLC
                     ISGTLSVRKKMIEQINNYDVIITSYDYIRRDFELYKPFKFEYIVLDEAQYIKNQATKN
                     ARAVKELHGTHRFALTGTPIENSLAELWSIFDFLMPNYLYNYNYFREHFERPIVRDED
                     KDAQIRLKKMVEPFILRRTKQEVLEELPDKIENNIKIAFNKEEENLY"
     gene            2669..2744
                     /locus_tag="PLK68_01435"
     tRNA            2669..2744
                     /locus_tag="PLK68_01435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2702..2704,aa:Thr,seq:cgt)
     gene            3417..3884
                     /locus_tag="PLK68_01440"
     CDS             3417..3884
                     /locus_tag="PLK68_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM89104.1"
                     /translation="MFKFMNKQDGGIVSPIDGKCINIEDVPDKVFSTRMMGDGFAIIP
                     SDNIVVSPVDGIIVMIPNSKHAFGIKTRSGVEILVHVGLDTVNLKGRGFEIFVKVGDK
                     VKKNMPILKFDDNIMDEMNIDKTTMVIFTAGYNQSIKLECYDREVHRGDTLIK"
     gene            3900..4769
                     /locus_tag="PLK68_01445"
     CDS             3900..4769
                     /locus_tag="PLK68_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89105.1"
                     /translation="MKVIKKINNNIAIAVDGENNEVIVFAKGIGYGNIPYEIKDLSVI
                     ERTFYDVDNRYYGLLKEIPNNIFNLVTKMVDVAKKNIEDELSPNLVFILADHINFAII
                     REKNGMDISLPYSYELEYEYPLLTKISKWMVKKTNEELDTHLPKGEVTSITMHFINAL
                     EGTNTKMHAGNIENKISKVIFNVTNIVEKHFNFNIDKKSFNYFRFKNHLKYFVQRKEA
                     REMFTDNNRELYESMIEKYPDTYDCISKIDNYFYQEYKEKCTTEELLYLLVHVNRLYI
                     KEDCHRKGITPEK"
     gene            4851..6242
                     /locus_tag="PLK68_01450"
     CDS             4851..6242
                     /locus_tag="PLK68_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89106.1"
                     /translation="MAKKYEKLAKTILDSIGGKENITYFQHCTTRLRFNLKDRSIVKI
                     SDIENVDQVLGSQWSNDELQIIIGPAVADAYAEICEFGNLQREEAIDEDLDGNAEKKK
                     VALKSVLMGIMDGISGCITPIIPMMIGGGMIKVFYMLANMVGILPETSSTYQILYWLG
                     DAFIYFFPVFLGATAAKKFGANQGVGMLLGALLIYPSFIAMATEGAAMSIFGLPVYVA
                     NYSTTVIPIILTVYILSKVEKLINRYCPDILKSFVVPTGSLLVMLPIMLVVTAPLGYY
                     IGTYVAEAVIWIYETIGFLGVSILCALLPLMVMTGMHTMMTPYWTSAFASLGYDPFFL
                     PAMILSNMNQFAATLAVSLKAKTKKVKSTAMSCAVTAIVGGVTEPAMFGITFKYKKPL
                     YAAMIGNAAGGLIAGLLKVACYAFPGSGGMFAVVTFAGPGNNLIFFLIAALVGMIITF
                     ILTYILGIDEKEV"
     gene            6246..7724
                     /locus_tag="PLK68_01455"
     CDS             6246..7724
                     /locus_tag="PLK68_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89107.1"
                     /translation="MAFPKNFLWGGATAANQFEGGWNEDGKLDSTADHFTLGSRTEPR
                     LFTEKIDEDKYFYPSHKASDFYHRYQEDIALLGEMGFKVYRMSINWSRIFPHGDDEEP
                     NQKGIEFYRNVFLELKKYNIEPLVTISHYEMPYHLAEKYDGWYARETIDFYLKYCQTL
                     FNEYKGLVKYWLTFNEINSLILGGNGYFSGGILSSSKKDMGAGVIEDLDTLSTVEQHN
                     DIVKQYQALHHQLVASALATRMAHEISGEYQIGCMIAGITQYPYSCRPEDMLLVQKER
                     RNIFYYCPDIQINGAYPRYAHRYLKENDIHINFGENDEDILKSGTVDFFTFSYYSTGC
                     VTTDKNLEKTNGNLIFGVANPYLEKSQWGWQIDPLGLRYFLNEIYDRYHIPIMVVENG
                     LGQDDKLEIDKTVHDDYRIEYMKQHIQAMSEAVDDGVELVGYTPWGCIDLVAASTGEM
                     IKRYGFVYVDVDNLGNGTYNRYKKDSFYWYKKVIASNGRKLD"
     gene            complement(7821..7896)
                     /locus_tag="PLK68_01460"
     tRNA            complement(7821..7896)
                     /locus_tag="PLK68_01460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7861..7863),aa:Lys,seq:ttt)
BASE COUNT         2829 a          997 c         1416 g         2912 t
ORIGIN      
        1 atacagattt tcttcttctt tattaaaagc aatcttaatg ttattctcaa tcttatcagg
       61 taattcttct aaaacttctt gttttgttct tcttaaaata aatggttcaa ccattttctt
      121 taaacgaatt tgagcatctt tatcttcatc gcgaacgatt ggacgctcaa aatgttctct
      181 aaaataattg taattatata aatagttagg catcaaaaaa tcaaaaattg accataactc
      241 tgcaagtgag ttttcaattg gagtacctgt tagtgcaaaa cgatgagtac catgtaactc
      301 ttttactgct ctggcatttt ttgttgcttg gtttttaatg tattgagctt catcgagaac
      361 aatatattca aatttaaatg gtttatataa ttcaaaatcc cttcttatat aatcatatga
      421 tgtaatgata acatcgtagt tgtttatttg ttcaatcatc tttttacgaa cggataatgt
      481 tccactaata cataggacat tgagatcgtt tgagaatttt tttatttcat cctgccagtt
      541 taatattagg gttgctgggg ttattacaat actagctttg tgattctttt tagcgtcttc
      601 taatacagta atcatttgta aggtttttcc taatcccata tcatcagcaa gaattcctcc
      661 gaatccataa tcgctcattg ttttgatcca tttataacca gtacattggt aatctcttaa
      721 aatatcactg aatggttcag gaacaggata attattgata ttgcagttag ttagatggtt
      781 gatgagttct tggaattgtt ggttacggtt aaaattaatt gtttcgctat cttgtattaa
      841 ttcgtttaag aataatgctt tggatttatc gactatgata gcgccatctt gaatattttt
      901 gttttcaaga tttaaatctt gaattaaatt ataggcttca tttaaagttt gatcttctaa
      961 attcacaaat tcaccatttt ttaaacgatg ataggctttg tgtttttggt atgattctaa
     1021 aatatctcgg atttcatctt ttgaaacatt aatactgtct aaatcaatct ctaatagatc
     1081 attttttaaa cggataccaa tgtttaaaga aatgttttta ggtttattaa tatttttaat
     1141 gtcttcactg acaaatacat cacaatatct atttaattta ggcaaaatat tttttataaa
     1201 atagtatata tcttcatcgt tattggtgat taaatacata ttcgttagtt catcatattg
     1261 cgcgtaatct tcaatcattg ctagtgctgt atcaacgttg agtggcagct tggtttcata
     1321 actgtcaata tatttaccgt caaataatat attaccttca tcatcacgat actcaagatt
     1381 gattgttaaa tcaccaaagt tattcaaatc aacgtaagta agtaaagtga tcacactagg
     1441 catataatcg tcgatattag ctcctgtaat ggcaatataa ggtaagattt gatcaatgat
     1501 atatttgcca aaataaccaa aatcttcttt tgcgacaatg agattttctt tatctaattt
     1561 agaaatcagt tttttagtaa ttttagacat tttaagactg taacgattta aaatgttttt
     1621 gtctaaataa tataaatatt ggttaccaat aattaattgg ccgcctaatg gcaaaagtga
     1681 tatttcaaag taatcttctt tatcttcgat gttgattgtt aaattattta gatcaatagt
     1741 ggtaaaaaac atattgatgt taagatttgt gagatatgtt tcaaaaaatt catcaatatt
     1801 atatttgtta atatcaatag aacgatatct ttcaatatca tcaataaatt cgctattatc
     1861 ccgaataaaa tcaatttgtt ttaatgcttc tgaactaaat gatttacgat tgtaatcggt
     1921 tgttaactga ctaccgtaag tatatacatt gtgataacga tagttatata tgaattcttt
     1981 aagatttttt attaagtaag ttttattttg accaattcga taactgatca agattcgatt
     2041 aaatgaataa ttaatattag gaattaaatc aatcaatgaa tcatttgtta gttgaggatc
     2101 ttgctgatta gtgtagtcgg taattaattt ttttgattct tcgagttttg tttgaattaa
     2161 tttttcttca cgaacgcgtt caaaggcttc aatttttgct ttgtaatcaa ttttttgatt
     2221 aaactgaaaa ggaatttcag acatttctaa actataaaat tttaataata gtaccccaat
     2281 atgaccacat gctaattcat cattatggca ataagggcaa tcacattcaa aattatttat
     2341 ttttcctcca gtatctacga cgatatgaca agtactacta cgaccatata tttcaacagt
     2401 accatcaatg tggtactcat ttccctgttt tagaacggac atgtttgtga ttacattatc
     2461 tttataataa ctgtgagcaa ttttccagtt tttttcatta aatatcctat ttatttcatc
     2521 atctttaata tacatgttct ttccctccga tagaatatta tatcattatt ttaatgatta
     2581 ccaaatagat aacattaaaa aaagataaat aaaaaaagat attttatgac aaggaataaa
     2641 gtgttataat atgaatgcgg ttacttatgc cggtatagct ctaacggtag agcaatccac
     2701 tcgtaatgga taggttgctg gttcaaatcc agttgccggc accataattt tatttcaata
     2761 actttatctg attatttaga aaagttatga atgtctatat atgaagcgat aaactgtatt
     2821 caatttgtcg ctttttcgtt ttatcatatt ttaaaagaaa gcagaaaatt aataaatgta
     2881 atataaagtt gtaatcgatt ttataaattc aaacatttaa ttatagattg attttttaat
     2941 tacatgaaga atatgtgaag aagtagatta tgattattat atatatctaa ttttatttga
     3001 tgatgaaatt aagaatatta tacaggattt taaaataaat gatggttcta cgtatacaga
     3061 tgcaagttct ttgacagagg gaatgatgga ctaaagaatt aacaactata gaaaaagctt
     3121 ttaggtttga ttagataaaa tgtgtatatg ctcaaatttt actagaatat catctgatta
     3181 aaaatgatat ttcaagatat ataaatattt tatgtacatt agctgatttt aaatgtatac
     3241 gataatttaa aaattaataa tatcattgat agttatcata tattattgac aataggttaa
     3301 ataattgtta ttatattggt gtaaaattga acagaaggat tgtaactctt tgaggcatga
     3361 cctattattt aggataatga atcaaagagt ttttttgtat aaaaggagga gttaatatgt
     3421 ttaaatttat gaataaacaa gatggaggaa ttgtttcacc aatagatgga aaatgtataa
     3481 atatagagga tgttccagat aaagtatttt caacaagaat gatgggtgat ggctttgcaa
     3541 ttattccatc agataatatt gttgtttcac ctgttgatgg aataattgtt atgataccaa
     3601 atagtaaaca tgcatttgga attaaaacta gaagtggggt tgaaatatta gtacacgttg
     3661 gtttggatac tgtaaactta aaaggtagag gatttgagat atttgtcaaa gttggcgata
     3721 aggtaaagaa gaatatgcct atattaaaat ttgatgataa tattatggat gaaatgaata
     3781 tagataaaac aactatggtt atatttacag caggttataa tcaaagtata aaattagaat
     3841 gttatgatag ggaagttcat agaggagata ctttgattaa atagaaggag gaatacttcg
     3901 tgaaagtcat caaaaaaata aataataata tagcaattgc agttgatggt gaaaacaatg
     3961 aagttattgt atttgctaaa ggaattggat atggaaatat tccctatgaa ataaaagatc
     4021 tctctgttat tgagcgaacc ttctatgatg ttgacaatag atattatggt ctacttaagg
     4081 aaataccaaa taacattttt aacttagtta caaaaatggt ggatgttgca aaaaagaata
     4141 ttgaagatga attaagtcct aatttagtgt ttattttagc tgatcatatt aattttgcta
     4201 ttattaggga gaaaaatggt atggatattt cgttaccata ttcttatgaa ttagagtatg
     4261 aatatccact attaacaaaa atatcaaagt ggatggtaaa aaaaactaat gaggaattag
     4321 atactcattt accaaaaggt gaagttacaa gtataactat gcattttatt aatgcacttg
     4381 aaggtacaaa tacgaaaatg catgcaggta atattgaaaa taagatatca aaggttatct
     4441 ttaatgttac taacatagta gaaaaacatt ttaattttaa tatagacaag aagagtttta
     4501 attactttag gtttaaaaat catctaaaat attttgttca aagaaaagaa gctagagaaa
     4561 tgttcacaga taacaatcgt gaattatatg agagcatgat agaaaagtat ccagatacct
     4621 atgattgtat cagtaagata gataattatt tttatcagga atataaagaa aaatgtacga
     4681 ccgaggaact attatattta ttagtccatg ttaatagatt gtatataaaa gaggattgtc
     4741 accgaaaggg catcacccca gaaaagtaat actttgatgg ggttttattt tactcattac
     4801 attatttggc gcgcaagata atgttttgta tataaaaagg aggagaaaaa atggcaaaga
     4861 aatatgaaaa acttgctaaa acgattttag attcaatcgg tggaaaagaa aatattacat
     4921 attttcaaca ttgtacaacc agattgcgtt ttaatttaaa ggacaggagt attgtgaaga
     4981 tatctgatat agaaaatgta gatcaggtat taggaagtca atggtccaat gatgaattgc
     5041 agatcattat tggacccgca gttgctgatg cttatgcgga aatttgtgag tttggcaatc
     5101 tccaaagaga agaagcaatt gatgaagatc ttgatggaaa tgcagaaaag aaaaaagtcg
     5161 ctttaaaatc tgtacttatg ggaattatgg atgggatatc aggatgtatc acgccgatta
     5221 ttccaatgat gattggtgga ggaatgatca aagtattcta tatgcttgcg aatatggtag
     5281 gaatcttgcc tgaaacaagc tcaacatatc aaattttata ttggttgggg gatgctttta
     5341 tctatttctt tcccgttttt ttaggcgcaa cagctgctaa aaaatttgga gcaaaccaag
     5401 gtgtaggaat gttgttaggt gcattgctta tttatccaag ttttattgca atggcaactg
     5461 aaggagctgc tatgtcaatt tttggattac cggtttatgt tgcaaattat tcaactaccg
     5521 ttattccaat tattttaact gtttatattt taagtaaagt tgagaaacta attaatcgat
     5581 attgtccaga tattttaaaa tcatttgttg tccctacagg ttctttatta gtcatgttac
     5641 caattatgtt agtagtaacg gcaccgttgg gatactatat tggaacttat gtggcagaag
     5701 cggttatttg gatttatgaa acaattggtt tcttaggggt ttctattttg tgtgcattgt
     5761 tacctttaat ggttatgact ggtatgcata caatgatgac accatattgg acttcggcct
     5821 tcgcctcttt aggatatgat cctttctttt taccagctat gattctttct aatatgaatc
     5881 aatttgcagc gactttagca gtatcattaa aagctaagac aaaaaaagta aaatctactg
     5941 ccatgtcgtg tgcggttact gcaattgttg gtggggtaac agaaccggct atgttcggga
     6001 ttacttttaa gtataaaaaa ccattgtatg cagcgatgat tggaaatgca gctggaggat
     6061 taattgctgg actattaaaa gttgcctgtt atgcatttcc aggatcagga ggtatgtttg
     6121 cagtagttac ttttgcgggt ccaggaaata atttgatatt ctttttaatt gctgcattgg
     6181 taggaatgat tattacattt atattaactt atattttagg aatagatgag aaagaggtgt
     6241 agggagtggc ttttccaaag aattttttat ggggtggcgc aacggctgct aatcaatttg
     6301 aaggtggttg gaatgaagat ggtaaattag attcaactgc tgatcacttt acattaggct
     6361 caagaacaga gccaaggtta ttcactgaaa aaatagatga ggataaatat ttttatccat
     6421 cacataaggc gagtgatttc taccatcgtt atcaagaaga tattgcttta ctgggtgaaa
     6481 tgggatttaa ggtttataga atgtcaatta attggagccg tattttcccg catggtgatg
     6541 atgaagaacc aaatcaaaag ggaattgaat tttataggaa tgtattttta gaattgaaaa
     6601 agtataatat tgaaccactt gtgacaattt cacattatga aatgccttat catttagcag
     6661 aaaaatatga tggctggtat gcacgtgaaa caatagattt ttatttaaaa tattgtcaaa
     6721 cacttttcaa tgaatacaaa ggacttgtta aatattggct aacgtttaat gaaattaata
     6781 gtttgattct tggaggtaat ggatatttct ctggtggaat tctttcgtca tcaaaaaagg
     6841 acatgggagc tggcgttatt gaagatttag atacattatc aacagtagaa caacataatg
     6901 atattgtgaa acaatatcaa gcattacatc atcaacttgt tgctagtgca ttggcgacca
     6961 gaatggctca tgaaattagt ggtgaatacc aaataggatg catgattgct ggaattacac
     7021 aatatccata ttcttgtcga cctgaagata tgttattagt acagaaagaa cgtagaaata
     7081 ttttctatta ttgtcctgat attcaaataa atggagcata tcctcgctat gcacatcgtt
     7141 atttgaagga aaacgatatt catatcaact ttggggagaa tgacgaagat attttaaaaa
     7201 gcggaacagt tgatttcttt accttttcat attattcaac tggttgtgta acaacagata
     7261 aaaatttaga aaaaacaaat ggtaatttaa tatttggagt tgctaatcca tatctagaaa
     7321 agagtcagtg gggttggcag attgatcctt tggggttacg ttatttttta aatgaaatat
     7381 acgaccgtta tcatattcct attatggttg ttgaaaatgg attaggacaa gatgataaat
     7441 tagaaataga taaaacggtt catgatgatt atcgtattga atatatgaaa caacatattc
     7501 aggccatgtc tgaagctgtt gatgatggtg ttgaacttgt tggttataca ccatggggat
     7561 gtattgattt agtggctgca tctactggcg agatgattaa aagatatggt tttgtttatg
     7621 ttgatgtaga taatttgggg aatggaacat ataatagata taaaaaagat tcattttact
     7681 ggtataaaaa agtaattgct tcaaacggaa gaaaattaga ttaagttaat attctgttta
     7741 ttcatttata agttcattga ttctttaaat tggaattaac cataaaaaag atgaacaaaa
     7801 gttcatctta gtgtttcaaa tggtgacccg caggcgattc gaacgcctga ccctctgatt
     7861 aaaagtcaga tgctctaccg gctgagctag cgggtcatag acacttaaca gtgccatata
     7921 aaaataacat agttttgaaa taatgtaaag ctattttggg aaaaaataaa ttaatttagc
     7981 aatgtttttc ttataatgat gaaaattgtt ataattattc agcataaaga gaacatgaat
     8041 tagtaaaaga actagctgat ataataaata gtcctaaaga ttttgatctt taggacttct
     8101 ttttgcttgg gcgggaaggt catatatttt tcgtagctct gagatcgttt gata
//
LOCUS       DAOMTA010000027         8135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_102_length_8135_cov_2.897772, whole genome shotgun sequence.
ACCESSION   DAOMTA010000027 DAOMTA010000000
VERSION     DAOMTA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102_length_8135_cov_2.897772"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..192)
                     /locus_tag="PLK68_01465"
     CDS             complement(7..192)
                     /locus_tag="PLK68_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRM89108.1"
                     /translation="MAKTSMKVKQQRPQKYKVREYTRCERCGRPHSVIRKFKLCRICF
                     RELAYKGEIPGVKKASW"
     gene            complement(210..749)
                     /gene="rplE"
                     /locus_tag="PLK68_01470"
     CDS             complement(210..749)
                     /gene="rplE"
                     /locus_tag="PLK68_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRM89109.1"
                     /translation="MNRLMERYQNDVVKSLVEKFNYSSSMQAPKVEKIVLNIGVGDAV
                     SNSKLLDEAVNELTLITGQKPVITRAKKSIAGFKLREGAPIGCKVTLRGERMYEFLDK
                     LVNISLPRVRDFRGVSNNSFDGRGNYTLGIKEQLIFPEINFDKVNKLRGMDIVFVTTA
                     KSDEEGRELLAQLGMPFKK"
     gene            complement(766..1101)
                     /gene="rplX"
                     /locus_tag="PLK68_01475"
     CDS             complement(766..1101)
                     /gene="rplX"
                     /locus_tag="PLK68_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRM89110.1"
                     /translation="MKIRVGDTVEVIAGKDKGKQGEVLQVLAKQDKVIVEGVNTVTKH
                     IKPSQADPEGGIVTREAPIHVSNVAFYDSKAKAPVKLGYKIVEKDGKKTKVRVNKKTG
                     AEVDKKKKK"
     gene            complement(1116..1484)
                     /gene="rplN"
                     /locus_tag="PLK68_01480"
     CDS             complement(1116..1484)
                     /gene="rplN"
                     /locus_tag="PLK68_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRM89111.1"
                     /translation="MIQNESRLKVADNTGAKEVLVIRNLGGSNRKFSNIGDVVVATVK
                     QAAPGGSVKKGEVVRAVIVRSRYGVGRENGSYIKFDDNACVIIKEDKSPKGTRIFGPV
                     ARELRDADFMKIVSLAPEVL"
     gene            complement(1519..1779)
                     /gene="rpsQ"
                     /locus_tag="PLK68_01485"
     CDS             complement(1519..1779)
                     /gene="rpsQ"
                     /locus_tag="PLK68_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRM89112.1"
                     /translation="MERNNRKVYTGTVVSTKMDKTITVQVETHVKHKLYGKRMKSTTK
                     FHAHDEENIASVGDTVKIMSTRPLSATKRFRLVEIVKKAETV"
     gene            complement(1791..1985)
                     /gene="rpmC"
                     /locus_tag="PLK68_01490"
     CDS             complement(1791..1985)
                     /gene="rpmC"
                     /locus_tag="PLK68_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRM89113.1"
                     /translation="MTVQEIKELDNAALLAKVEEYKKELFGLRFQQATGSLENTARIR
                     TVRKSIARIKTIIRERELNQ"
     gene            complement(1988..2398)
                     /gene="rplP"
                     /locus_tag="PLK68_01495"
     CDS             complement(1988..2398)
                     /gene="rplP"
                     /locus_tag="PLK68_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRM89114.1"
                     /translation="MLMPKRTKYRRPHRVKYEGKAKGGTEVSFGEYGLQATEGAWITS
                     RQIEAARIAINRRLNRGGQVWIRIFPHLAKTKKPLEVRMGSGKGSPEEWVAVVKTGRV
                     LFEVAGVDEELAREALRLASHKLPIKCKIIGKGE"
     gene            complement(2401..3054)
                     /gene="rpsC"
                     /locus_tag="PLK68_01500"
     CDS             complement(2401..3054)
                     /gene="rpsC"
                     /locus_tag="PLK68_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRM89115.1"
                     /translation="MGQKVSPIGLRVGVNRNWDSRWYANDQDFAGLLHEDIKIREYLL
                     KKLKTASVAKVEIERSKNRVTIFVHTSRPGVVIGKDGEAVDALRKEVSKMVKDKQVFI
                     NIVEIKNPDVVAQLVANNIAEQLENRASFRTVQKRAIQRAMRAGTKGIKTSVSGRLGG
                     ADMARAEGYSEGNVPLHTLRADIDYATAEADTTYGKLGVKVWICKGEILPEKKKGDK"
     gene            complement(3066..3398)
                     /gene="rplV"
                     /locus_tag="PLK68_01505"
     CDS             complement(3066..3398)
                     /gene="rplV"
                     /locus_tag="PLK68_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRM89116.1"
                     /translation="MEARAQAKMIRVSPQKARLVVDLVRGKQVKEALGMLEYVNKSAT
                     PAIIKVVKSAAQNAVYNEGAEAEKLYIKEIYVDEGPTLKRFAARAKGSGTRILKRTSH
                     ITCVVEER"
     gene            complement(3418..3696)
                     /gene="rpsS"
                     /locus_tag="PLK68_01510"
     CDS             complement(3418..3696)
                     /gene="rpsS"
                     /locus_tag="PLK68_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRM89117.1"
                     /translation="MARSLKKGPFVDGHLMKKVEALNAAGKKEVIKTWSRRSTIFPQF
                     IEHTFAVYNGREHIPVYVTEDMVGHKLGEFAPTRTYHGHGADDKKAGK"
     gene            complement(3715..4548)
                     /gene="rplB"
                     /locus_tag="PLK68_01515"
     CDS             complement(3715..4548)
                     /gene="rplB"
                     /locus_tag="PLK68_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRM89118.1"
                     /translation="MAIKTYKPVTNGRRNMTSLTYEEITTNKPEKSLVAKVSKNGGRN
                     NQGVITTRHHGGGHKRKYRIIDFKRNKDDIVGTVATIEYDPNRSANIALINYADGEKR
                     YILAPKGLEVGSKIVSGENADIKVGNALPMGNMPEGTVIHNIEMQPGKGGQIARSAGV
                     SAQILGKEERYVIVRLASGEVRKLLAVCRATVGVVGNEDHGLVNYGKAGRMRWKGVKP
                     TVRGSVMNPNDHPHGGGEGRTSIGRKAPMTPWGKKAMGVKTRKNKKASTKLIVRRRNS
                     K"
     gene            complement(4601..4891)
                     /gene="rplW"
                     /locus_tag="PLK68_01520"
     CDS             complement(4601..4891)
                     /gene="rplW"
                     /locus_tag="PLK68_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRM89119.1"
                     /translation="MAHITDVLKKPVLTEKTMTLQANENKYTFDVDVNANKVEIKQAV
                     EAMFGVKVESVNVMNVKPKTKRMGRYEGKTNRRRKAIVKLAEGNEINYFGEE"
     gene            complement(4891..5514)
                     /gene="rplD"
                     /locus_tag="PLK68_01525"
     CDS             complement(4891..5514)
                     /gene="rplD"
                     /locus_tag="PLK68_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRM89120.1"
                     /translation="MLTVKVYNQEGSEVKDLELNEAVFGIEPNKQALFDMVLLQRASL
                     RQGTHKVKNRTEVRGGGKKPWRQKGTGRARQGSIRAPQWRGGGVVFGPTPRSYKFKLN
                     RKVRRLALKSALSTKINDNEFMALEAIKFDAPKTKEMVKVLANLEAPVKTLIVVDEIC
                     PNVARSANNIPGVKLLDAKHVNVYDILNSNKLIMTEAAIKSVEEVLG"
     gene            complement(5536..6264)
                     /gene="rplC"
                     /locus_tag="PLK68_01530"
     CDS             complement(5536..6264)
                     /gene="rplC"
                     /locus_tag="PLK68_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRM89121.1"
                     /translation="MKGILGRKIGMTQVFTTDGLLIPVTVVEADANVVLQKKTVATDG
                     YDAIQVGFEDKREKLANKAELGIVKKANTAPKRFIKEFRYDEMMSYEVGDKITVDSFV
                     AGEVVDVTGTSKGKGYQGVIKRHGQRIGPKGHGSGAHRIVGSMGPIAPNRIAPGKKLP
                     GQMGHVTRTVQNLEVVAVDVENNLLLIKGSVPGPKKGLVIVKSGIKAAGKVNEAHELV
                     DFTPVVEETKEADAATETPVEAAE"
     gene            complement(6286..6594)
                     /gene="rpsJ"
                     /locus_tag="PLK68_01535"
     CDS             complement(6286..6594)
                     /gene="rpsJ"
                     /locus_tag="PLK68_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRM89122.1"
                     /translation="MANNKIRIRLKSFDHKILDNSAEKIIGAAKKSGAQVVGPVPLPT
                     EKEIYTVLRAVHKYKDSREQFEIRTHKRLIDIVNPTQETVDILTRLELPSGVDIEIKL
                     "
     gene            complement(6807..7394)
                     /locus_tag="PLK68_01540"
     CDS             complement(6807..7394)
                     /locus_tag="PLK68_01540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM89123.1"
                     /translation="MTRIYLLRHGQTLFNQKNLIQGSCDSKLTAKGIAQAKAVKEYLD
                     TINFAAVYCSTSERTLDTATYATGGKYPIYPCKEFKEIDFGVVEGEDGSQLFKGMKHD
                     NFVSLLLNEGWSSLEGESGFDFTKRIFTKLDEITAQYPDENILIATHGGTILNTVVNI
                     DHNFVNMDGPANCSITIIDCHGEYRVIEYNLQVAQ"
     gene            complement(7405..8025)
                     /locus_tag="PLK68_01545"
     CDS             complement(7405..8025)
                     /locus_tag="PLK68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRM89124.1"
                     /translation="MKVLLINGSFRRNGCTFTALNEVAKALNTNGIDTEIFHIGTKAI
                     RGCMGCGVCHEKGECIDNSDAVNECLKLFEAADGIVIGSPVHYASSSGMIASFMDRLF
                     YASRFDKRFKVGATVVSCRRGGASATFDQLNKYFTIAQMPIVSSQYWNSIHGNTPEEV
                     KQDFEGMQTMKVLGNNMAFLLKSIALGKEKYGLPDAELKIATNFIR"
BASE COUNT         2238 a         1698 c         1117 g         3082 t
ORIGIN      
        1 atctaattac cagcttgctt tctttacacc tggaatttca cctttgtaag ctaattcgcg
       61 gaagcaaatt ctacaaagtt tgaattttct aattactgaa tgtggtcttc cacaacgttc
      121 acaacgtgtg tattcacgca ctttgtattt ttgaggacgt tgttgtttca ctttcattga
      181 tgtttttgcc atatttttgt gagtcctcct tatttcttga acggcattcc taattgagct
      241 aataactcgc gaccttcttc atcgctctta gctgtagtta cgaatacgat atccattcct
      301 cttaatttgt ttactttatc gaaattgatt tctgggaaaa ttaattgttc tttaatacct
      361 aaagtgtaat ttcctcttcc atcaaatgag ttatttgata cacctctaaa atctcttaca
      421 cgtggcaatg agatatttac taatttatct aaaaattcat acattctttc accacgtaaa
      481 gttaccttac atccgattgg agcaccttca cgtaatttaa atccagcgat tgatttttta
      541 gctctagtga taactggttt ttgtccagta attaatgtta attcattaac agcttcatct
      601 aataatttag agttagaaac tgcatcacca acaccgatat ttaaaacgat tttttcaacc
      661 tttggagctt gcatgctaga agaataattg aatttttcta ccaatgattt tactacgtcg
      721 ttttgataac gttccattaa acggttcatt ctttatcctc ctccattatt tctttttctt
      781 tttatctact tctgctccag tttttttgtt aactcgaact tttgttttct taccgtcttt
      841 ttcaacgatt ttatatccaa gtttaacagg agctttagct tttgaatcat agaatgcaac
      901 attagaaaca tgaataggag cttctcttgt tacaattcca ccttcaggat ctgcttgtga
      961 aggtttaata tgtttagtta cagtattaac tccttcaacg attactttgt cttgttttgc
     1021 aagaacttgt aacacttcac cttgttttcc tttatcttta cctgcgataa cttctacagt
     1081 atcacctaca cggattttca tgttttaagt tcctcctata atacttctgg agctaatgat
     1141 acgattttca tgaaatcagc atctcttaac tctcttgcaa ctggtccgaa gatacgagtc
     1201 ccttttggag atttatcttc ttttataatt acacaagcgt tgtcatcaaa tttaatataa
     1261 gaaccgtttt ctcttccgac tccatatcta cttcttacga taaccgctct tactacttca
     1321 ccttttttta ctgaaccacc tggtgctgcc tgtttaactg ttgctacaac aacatcaccg
     1381 atgttactaa atttacgatt agaaccacct aaattacgga ttactaatac ttctttagct
     1441 ccagtgttgt cagcgacttt aagtctactt tcgttttgga tcattcttgg ttacctcctt
     1501 ttagagtttt aatatatatt aaacagtttc tgctttttta acgatttcta ctaaacggaa
     1561 acgttttgtt gcagataatg gtcttgttga catgattttt actgtatcgc caacactagc
     1621 gatattttct tcatcatggg catgaaactt agttgttgat ttcatacgtt taccatataa
     1681 tttatgtttt acatgtgttt ctacttgcac tgtaatagtt ttatccattt tagtagatac
     1741 aactgtacca gtataaactt tacgattgtt tctttccatt gtatctcctc ctattggttc
     1801 aattctcttt ctctaataat tgttttaatt ctagcgatag atttacgaac cgttctaata
     1861 cgtgctgtgt tttctaaact tcctgtagct tgttggaaac gaagtccaaa tagctctttt
     1921 ttgtattctt ctactttagc aagtaaagca gcgttatcta attctttgat ttcttgaact
     1981 gtcatgacta ttcacccttt ccaatgattt tacatttaat aggtaattta tgagatgcaa
     2041 gtcttaaagc ttctctagca agttcttcat caacacctgc aacttcgaaa agaacacgac
     2101 cagttttaac aactgctacc cattcttctg gagaaccttt cccagatccc atacgtactt
     2161 ctaatggttt tttagtttta gctaagtgag ggaaaattct aatccatact tgtccaccac
     2221 ggtttaaacg acggttgata gcaatacgag cagcttcgat ttgacgagaa gtgatccaag
     2281 caccttcagt tgcttgtaaa ccatattcac caaatgatac ttcagttccg cctttagctt
     2341 taccttcata tttaacacga tgaggtcttc tgtattttgt tcttttaggc atcaacatga
     2401 ttatttatcc cctttcttct tttcaggaag gatttctccc ttacaaatcc aaactttaac
     2461 tcctaattta ccataagtag tatctgcttc tgcagtagca taatcaatat cagctcttaa
     2521 agtatgaaga gggacattcc cttcagagta accttcagca cgagccatat cagctccacc
     2581 taaacgtcct gaaacacttg ttttaattcc tttagttcca gctctcattg ctctttggat
     2641 agcacgtttt tgaactgttc taaaagaagc acgattttct aattgttccg caatattatt
     2701 agcaactaat tgtgctacaa catcagggtt tttgatttcc acaatattga taaacacttg
     2761 tttatctttt accattttac taacttcttt tcttaatgca tcaactgctt caccatcttt
     2821 accgattaca actcctggtc tagatgtgtg aacaaagata gtaacacgat tttttgatct
     2881 ttcgatttca actttagcta cagatgcagt cttaagtttt tttaataggt attcacgaat
     2941 cttgatatct tcatgtaata aacctgcgaa atcttgatcg ttagcatacc atcttgaatc
     3001 ccaattacgg ttaacaccaa ctcgtaatcc gattggactt actttttgac ccataatgtg
     3061 acctcctatc tttcttctac cacacaagtg atgtggctag ttctttttaa aattcttgtt
     3121 ccgctacctt tagctcttgc agcgaatctt tttaatgtag gaccttcgtc tacatagatt
     3181 tctttaatat ataacttttc tgcttctgca ccttcgttat aaacagcgtt ttgtgcagca
     3241 gattttacaa ctttaatgat tgcaggagtt gcacttttat ttacgtattc taacatacca
     3301 agtgcttctt ttacttgctt ccctctcact aagtcaacga ctaatcgagc tttttgaggt
     3361 gaaacacgaa tcattttagc ttgtgctctt gcttccatgt tttaaaatct cctctctcta
     3421 ttttccagct tttttatcat cagccccgtg accgtgataa gttcttgttg gagcgaattc
     3481 tcctaattta tgaccgacca tatcttcagt tacataaact gggatatgtt ctcttccgtt
     3541 atacactgca aatgtatgct caataaattg agggaagatt gttgaacgtc tagaccaagt
     3601 tttgataact tcttttttac cagcagcgtt taatgcttca acttttttca ttaagtgtcc
     3661 atcaacaaat ggtccctttt ttaaactacg tgccattatt tcttcctcct cttattattt
     3721 agaatttctg cgacgtacaa ttaatttagt agacgctttc ttattttttc tagttttaac
     3781 acccattgct tttttacccc aaggtgtcat tggagcttta cgtccgatag aagttctacc
     3841 ttcaccacca ccatgagggt gatcgttagg gttcattaca gaaccacgta ctgtaggttt
     3901 aacacctttc catctcatac gtccagcttt accatagtta actaatccgt ggtcttcgtt
     3961 tccaacaacc ccaacagtag ctctacatac agctaataat ttacgtactt caccagatgc
     4021 taatcttacg attacatatc tttcttcttt acctaagatc tgagcagaca caccagcaga
     4081 acgtgcgatt tgtccacctt taccaggttg catttcgata ttgtggatta cagtaccttc
     4141 aggcatattt cccattggta aagcatttcc tactttaata tctgcgtttt caccagatac
     4201 gatcttactt ccaacttcta atccttttgg agctaaaata tatctttttt caccatctgc
     4261 atagttaatt aatgcgatgt ttgctgatct atttggatca tattcgatag ttgctacagt
     4321 tccaacgata tcatctttat tacgtttgaa gtcaataata cgatatttac gtttatgtcc
     4381 tccaccatga tgacgtgttg taataacacc ttggttatta cgtccaccgt ttttgcttac
     4441 ttttgcaact aaagatttct caggtttatt tgttgtaatt tcttcataag ttaatgaagt
     4501 catattacga cgaccattcg tcactggctt ataagtttta attgccatct ttagtttcct
     4561 cctagttatc tggaattatg tattccaata ttttagttaa ttattcctca ccgaaataat
     4621 tgatttcgtt accttcagct aatttaacga tagcttttct tctacgatta gttttacctt
     4681 cgtatctacc cattctctta gttttaggtt taacgttcat aacattaaca ctttcaactt
     4741 tcacaccaaa cattgcttca acagcttgtt tgatttcaac tttatttgca ttaacatcta
     4801 catcaaacgt gtatttgttt tcgtttgctt gaagagtcat tgttttttca gtaagtactg
     4861 gtttttttaa tacatctgta atgtgtgcca ttatcctagt acctcctcaa cagatttaat
     4921 agcagcttca gtcatgatta acttgttgct gtttaagata tcataaacat ttacatgttt
     4981 tgcatctaat aatttaacgc ctgggatgtt gttagcactt ctagcaacgt ttggacaaat
     5041 ttcgtcaaca acgatcaatg tcttaactgg tgcttctaaa tttgctaata ctttcaccat
     5101 ttcttttgtt tttggagcgt cgaatttaat agcttctaaa gccatgaatt cattatcgtt
     5161 aattttagta gatagtgctg atttaagcgc taatcttcta actttacgat ttaatttaaa
     5221 tttataactt ctaggtgttg gaccaaatac aactcctcct ccacgccatt gtggtgcacg
     5281 gattgatcct tgtctagctc taccagtacc tttttgacgc catggttttt taccaccacc
     5341 acgaacttca gtacggtttt ttactttatg agtaccttgt cttaatgatg ctctttgtaa
     5401 taagaccata tcaaataatg cttgtttatt tggttcaatc ccaaatacag cttcatttaa
     5461 ttctaaatct tttacttcag aaccttcttg gttatatact ttaactgtaa gcatatcgaa
     5521 tcctccttcc ctaaattatt cagctgcttc aactggtgtt tcagttgctg catctgcttc
     5581 tttagtttct tctactactg gtgtaaagtc tactaattca tgagcttcgt ttactttacc
     5641 agctgcttta attccagatt ttacaattac taatcctttt ttaggtccag gtactgaccc
     5701 tttgattaat aataaattat tttctacatc aacagctacc acttctaagt tttgtactgt
     5761 tcttgttaca tgtcccattt gtcctggtaa ttttttacct ggagcaattc tgtttggagc
     5821 gattggtccc attgaaccaa caattctatg agctcctgaa ccatgtcctt taggaccgat
     5881 tctttgacca tgtcttttaa taacaccttg ataaccttta cctttagaag ttccagttac
     5941 atctacaact tcacctgcta cgaagctatc aacagtaatt ttatctccaa cttcataact
     6001 catcatttcg tcataacgaa attctttaat gaagcgctta ggagctgtgt tagctttttt
     6061 cacaatacct aattcagctt tattagctaa tttttctctc ttatcttcga atccaacttg
     6121 gattgcatca tatccatcag tagcaactgt tttcttttgt aatacaacgt tagcatcagc
     6181 ttctactaca gttactggga ttaataaacc atcagttgtg aagacttgag tcattccaat
     6241 cttacgacct aagattcctt tcatgagtta caccttttac ctttcttata atttaatttc
     6301 gatgtctaca ccacttggta attctaatct tgttagaatg tcaactgttt cttgtgtagg
     6361 attcacaatg tcgattaaac gtttgtgagt tctgatttca aattgttcac gagaatcttt
     6421 atacttgtga accgctctta atactgtata aatttctttt tcagttggta gaggtacagg
     6481 acctactact tgagctccag atttcttagc agcaccaata attttttctg ctgagttatc
     6541 tagaattttg tgatcaaatg attttaatct aattctaatt ttgttatttg ccatgatttt
     6601 tctcctttca acttatagga tgtgacttac tcaatacgaa ttccctgaaa catgccgttt
     6661 catgacaacg cctttcggtg tttcagcaac ctcgcacatc tttgcaacgt caacggtagt
     6721 attttaacaa aaaaaagccc ctattgcaag gctttttttc atttatttta attaattttt
     6781 cttcatttaa tcatgctatt tttaatctac tgagcaacct gtaagttata ttcaatcact
     6841 cgatattcac catgacaatc aataatggtg atcgagcaat tagctggacc atccatatta
     6901 acaaaattat ggtcaatgtt aactactgta tttaaaatcg tgcccccgtg agtcgcaatt
     6961 aatatattct cgtcaggata ttgagcagtt atctcatcta gttttgtgaa aattcgtttc
     7021 gtaaaatcaa aaccgctttc tccttctaaa gaactccatc cttcatttaa cagtaatgat
     7081 acaaaattat catgtttcat tcctttaaat aactgactac catcttcacc ttcaactact
     7141 ccaaaatcaa tttccttgaa ttctttacat gggtagatcg gatattttcc accagttgca
     7201 tatgtcgcag tatccaatgt cctttcactt gttgaacaat aaacagctgc aaaatttatt
     7261 gtatctaaat attccttaac agctttagcc tgggctattc ctttagctgt taatttagaa
     7321 tcacaacttc cttggattag atttttttga ttaaataaag tttgcccatg acgtaataaa
     7381 taaattcgtg tcattctaat actcctatct gataaaatta gttgctattt ttagttctgc
     7441 atcaggtaaa ccatattttt ctttacctaa agcaatactt ttgagtaaga atgccatatt
     7501 attacctaat actttcattg tctgcattcc ttcaaaatct tgttttactt cttcgggtgt
     7561 attaccatgt atgctattcc aatattgcga agatacaatc ggcatttgag cgattgtaaa
     7621 atatttattt aattgatcaa aagtagcact ggcaccacct cggcgacaac taactacagt
     7681 ggcaccaact ttaaaacgtt tatcgaatct tgaagcatag aataggcgat ccataaagga
     7741 agcaatcatt ccagaagagc ttgcataatg aacaggactt cctatcacaa ttccatcagc
     7801 agcttcaaat agttttaaac actcattaac agcatcactg ttatctatac attcaccttt
     7861 ctcatggcaa acaccacatc ccatgcaacc cctaatagct tttgttccaa tatgaaatat
     7921 ttcagtatca ataccattag tatttaatgc tttagcaact tcattcaaag ctgtaaaagt
     7981 acagccattt cttctaaaac ttccattaat taataatact ttcataccac atcctccatc
     8041 tttaataaat ataatagcgc aatctataat aattgcaaag aaaaaaggat acgcagaggt
     8101 atccgtaaat aaaaaaagaa ccggcagctt gctat
//
LOCUS       DAOMTA010000028         8069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_105_length_8069_cov_2.671450, whole genome shotgun sequence.
ACCESSION   DAOMTA010000028 DAOMTA010000000
VERSION     DAOMTA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_8069_cov_2.671450"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2234
                     /locus_tag="PLK68_01550"
     CDS             <1..>2234
                     /locus_tag="PLK68_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM89125.1"
                     /translation="GTAVLYDANGKSSKDPGVGYSVFLDPGKEVVLQEVRAPDYYGII
                     HEWWYVGEIKAQHISEIEVQNYLLTNPIGYKYNEAEKLLAGATMGLFSTETGADRLNK
                     LSQTEIDEVIADSEKWAEYDLVSTAVSTDKYFKFENIDSTQTYYVTELKQPAGYNKNT
                     DIRKVTVEVNGKVYFLKDADTNETVTFINYKYRQIWVSKVLKFAGEQSALTGINFKVY
                     PVVESNESVADAFKIGDKWYIKTGEEVTYTTGTISSGKTGSFMTAPSPAGLYVVEEDI
                     TSLPEHVVAPDTTKYLVNLGFDTDNTDLYSDQADASNVIVNTSNYGKLALNKVSSTNS
                     NTYIKATFNIYAKNDEAGHDYSKDTPISTITTNGNQNAVLTDYLKPGNYVLVESLIST
                     DGYVLNPTPREFTIEADKVTGLDGSVYTTVSDAVKNPLVVTNVPKGSVELTKQGTYLD
                     ADGLTNPVALSGVNFNVYKSVKLADGTLDLSSTNLVGTAVSQSNGKLQFKVNGTDVTS
                     KKWLEAGDYILQETTVGTTNKNNGFADYAYLGKFTITPGDITKEIVKVDENGNESGKA
                     DNVISNTSSYGKLEITKVDHYDKTKKLADVVFEVFNESGILVDTIKTNKNGVARTKLL
                     PAGEYTLKEKSTNNDYFLNDTLYGPYTVEALKVTGTDGKIEITNMMKQSLKVIKKDSH
                     TGKEIDKLDGTTFSLYDAQTDGNLVATATYQKDKGIVFDNLRPNTTYYLQEDISPNGY
                     VIKD"
     assembly_gap    2235..2244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2245..7977
                     /locus_tag="PLK68_01555"
     CDS             <2245..7977
                     /locus_tag="PLK68_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRM89126.1"
                     /translation="RIKVTTKTNGEVTTIEVENEPLGSIKIEKISEWSIYGANDSKTT
                     RFPLAGAEFTLYKDVNGDSKLQDDEKVNGIVKNSDTLGIVIFDNLEKGKYLFVETKVP
                     DGYDETVMNDVYAVEVTEGEQNIVYTEAGKTDDAQNHGPIINTTTAGKFTFTKTDPEG
                     KGLSGATFQLMKKNTDGDYENYLDSFTIENTDGKFESSVIETGKYQLVEITAPDNFEI
                     MQPIEFTIEAGKVTTVTSKDANTVVNQALGKVVITKYDDRSNYSNVGNKVLEGVEFGL
                     YQESGDLVQTVKTTGKDGKIIWTDVPAGNYYVKEVATLDGFELDTTVYPVTVESGQST
                     VKEYLPNTDGKIINHSNMGKIVVKKTSDANENLAGAVFKITNPNDSGFVPIIITTNED
                     GIAKTELLPANKEGTTYVVTEIKAPDGYTLDDKYHDIEQSVKVYPVQDETVILSEQSK
                     NYLEFKNKKQSDIMDIDGKIIKYIDGDNNTLLTAKTVETPLLDAADTETFLLREYAQA
                     KNDVPVRSVEVEDSLIKMYYYDANGNSVEYMPDAPYVVNTVTVHSATDKNGNKIQARV
                     YYQTAGSDEWTTNNSLVADVSTNSQGISLEGLNALKIKVVYTNVVSKDLIAENFNATG
                     IDVNVTFNKRPSDATVHEVRRVSNQTSVTYNYTLKDNEGNDQNAQYTKVSDIVNLYYP
                     LKESVTPRVSLGIVSGEKDKTYKPGETIESVITVKNESTKENEIIEQPIISFDLPIGM
                     SLNNNSYSIGDLQTRFQVIVFEHDGDTSGKALEPDEYTVTYTNDVPARVVENNQLVET
                     TSKTKKITIDLGEDFKFKPGMKIQISYKGTSSINDTSTTLWAPAYFTSGKIIALSAEN
                     PYGNSFTVESATGSSYNTLVADSVLDEITNKPDETGDGLKYPNSNGIIKINEQNYLSI
                     YKQVKGIYDDDYLNNNQIAKTAPGEDLDYKIVFKNGEKSDRAVSKARVVDILPFEGDS
                     LVNRTNDNYTARVTNLDKSPILNYVDCLTPGVSYKVYYCVGESEDTKWDEWKQDARTS
                     KTASEELPIVYGNMDDDDWTSGAHQWIEASNDIDLRLVSAIAVEFDFSNAPLEPNQSI
                     ELHVNMSAPEYSTSDLEKVSGKLMSNSALVAVKRTGLDTVNDSDIVENLEVKAEIALP
                     KGTIGDYAFYDMNRDGLQDSNDLPVQGLNVTLHKFVTTINQNEGKVTKELDSETTLTD
                     ASGKYEFTNQDCNVLKDGKTDSSSTNPNDYVGNKYYQYQVEFAIPEDESLYKYEPTQR
                     YAQDVDGKLQIEADSNIGNDLNKDDYCKTELFTLTATKQADGSLVGETNLTLDAGYVA
                     LGSLGDFVWFDENKNGIQDPEETGIKDVIVNLYTVTNGEVSGTPEKTTRTDENGYYLF
                     TDLEDGSYVVEFDIRGVKPTMGGGHAERFYFTKNGGTTDSAIDSNPTITDENENTLIA
                     RSEVIDLAYHTSDMTIDAGLTVYSAISGVAFEDRDYSDIQNYQDSDGKDVDIKLPGTI
                     VELYRVDEEFGIENNIPTELVASTVVGEDGSYLFDYLDSGTYVVKFTYPEGYKVIEAD
                     VGDDDTLDSDVAYHIETNENRMSGYTGVINIPVNTRVDNVDGGARLYSSLGDYVFKDV
                     NVDGLQDETDIVMPDVPVYLFARQKGEEVWTSIANTITDQDGRYRFDNLKGSTYTGIE
                     YRVIFDLPLTTKLTVPYAGDDPELDSNALNEYVPGLGFPTQTIDLAYNTVDLTWDAGI
                     VQSKGSVGDYVWYDTNVNGIQDENGTGIEGIKVILETNLTGNIADENDWQEVGTTYTN
                     SQGYYIFNELSEGYYRVKFEIPSKYKVTLSTQGEDSAEDSDGIYSEDGVWYYTRSFYL
                     DQDGYDMTWDCGVYDPTDTKLTKTGSTATGDSSDINGYVVLGFSSILALGAVYFVSNK
                     KRKAKKAEKI"
BASE COUNT         2963 a         1001 c         1575 g         2520 t
ORIGIN      
        1 gggaacagct gttttatatg atgcaaatgg taaatcctca aaagatcctg gggttggtta
       61 ttcagttttc cttgatccag gtaaagaagt agtattacaa gaagtaagag ctccagacta
      121 ttatggaata atccacgaat ggtggtatgt tggtgaaatc aaagcacaac atattagcga
      181 aatcgaagta cagaactatt tattgactaa tccaatcggt tataaataca atgaggcaga
      241 aaaattactt gctggtgcta ccatgggatt gtttagtact gaaactggtg cagatagatt
      301 gaataagtta agtcaaactg aaatagatga ggtaattgct gattcagaaa aatgggcaga
      361 atatgatttg gtaagtacag ctgtatctac tgataagtac ttcaagtttg aaaatattga
      421 ttctactcaa acttattatg ttactgaatt aaaacagcca gctggctata ataaaaacac
      481 agatattcgt aaagtgactg ttgaagtcaa tggtaaggtg tattttttaa aggatgctga
      541 tactaatgaa acggttacat ttataaatta taaatatcgt cagatctggg ttagtaaagt
      601 tttaaaattt gcaggtgaac aatcagcatt aacaggaatt aatttcaaag tatatccagt
      661 tgtagaatcg aatgaaagcg ttgctgatgc atttaaaata ggtgataagt ggtatattaa
      721 aacgggagaa gaagtaactt atacaactgg tactatttct tcagggaaaa caggcagctt
      781 tatgacagct ccttctccag ctggattata tgtagttgaa gaagatataa cttcattacc
      841 agagcatgtc gttgcaccag atactactaa atatttagtt aatttaggtt ttgataccga
      901 taatacggat ctctattcag atcaagcaga tgcttctaat gtgattgtta atactagtaa
      961 ttatggtaaa ttagcattga ataaggtgtc tagtactaat tcaaatactt atataaaagc
     1021 gacattcaat atttatgcta aaaatgacga ggctggacat gattatagta aagatacacc
     1081 aatttcaact atcacgacaa atggtaatca aaatgctgta ttgacagact atttgaaacc
     1141 tggaaattat gtgctggttg aaagtttgat tagtactgat gggtatgtat tgaatccaac
     1201 accacgtgaa tttacaatcg aagcagataa agttactggt ttagatggta gcgtttatac
     1261 tacggtttca gatgctgtca agaacccatt ggttgtaaca aatgtaccaa agggtagtgt
     1321 tgaattaaca aaacaaggaa cctatctaga tgctgatggt ctaactaatc cggttgcttt
     1381 aagtggagta aactttaatg tatataaatc agttaaatta gctgacggta ccctagattt
     1441 atcgtctact aatttagttg gaacagcagt atcacaatct aatggtaagc ttcagttcaa
     1501 agttaatgga actgatgtta ctagtaaaaa gtggttagaa gcaggtgatt acatcttaca
     1561 agaaactact gtaggtacta caaataaaaa taatggtttt gcggattatg catatcttgg
     1621 taagtttacc attacacctg gagatattac aaaagagatt gtcaaagttg acgaaaatgg
     1681 taatgaatca ggtaaagccg ataatgttat cagtaatact tcatcttatg gcaaattaga
     1741 aattacgaaa gttgatcatt atgataaaac aaagaaatta gctgatgttg tttttgaagt
     1801 gtttaatgaa tccggaatct tagtcgatac aattaaaaca aataaaaatg gtgttgctag
     1861 aacaaaatta ttaccggctg gggaatatac actaaaagaa aaatctacta ataacgatta
     1921 tttcttaaat gatactcttt atggtcctta cacagtagag gctctaaaag ttactggtac
     1981 tgatggaaaa attgaaatta ctaatatgat gaaacaatca cttaaagtaa ttaaaaaaga
     2041 tagtcatact ggaaaagaaa ttgataagtt agacggtaca acatttagtt tatatgatgc
     2101 tcaaacggat gggaatttag ttgctactgc aacgtatcaa aaagataaag gaattgtttt
     2161 tgataattta cgcccgaaca ctacttatta tttacaagaa gatatatcac caaatggtta
     2221 tgtaatcaaa gattnnnnnn nnnncgaatt aaagttacaa ccaaaactaa tggagaagta
     2281 acaactattg aagttgaaaa tgaaccttta ggaagtatta aaatcgaaaa gatatcagaa
     2341 tggtctattt atggagcaaa tgatagtaag actacacgtt tcccattagc tggagctgaa
     2401 tttactttat ataaagatgt aaatggtgat agtaaactgc aagatgatga aaaggttaat
     2461 ggaatagtaa aaaatagtga tactttagga attgtcatct ttgataattt agaaaaagga
     2521 aaatatttat ttgttgaaac gaaggttcct gatggttatg atgaaacagt tatgaatgat
     2581 gtatatgctg ttgaggtaac tgaaggagaa caaaatattg tttataccga agctggaaaa
     2641 acagatgatg ctcaaaatca tggtcctatt attaatacga caacagctgg taagtttaca
     2701 tttactaaaa ctgatcctga aggaaaaggt ttaagtggag ctactttcca attgatgaaa
     2761 aagaatacag atggagatta tgagaattat ttggattcat ttacgataga aaatacagat
     2821 ggtaaatttg aatcttcggt aattgaaact ggtaaatatc agttagtaga aatcactgcg
     2881 ccagacaatt tcgagattat gcagccaatt gaatttacga ttgaagccgg caaagtgaca
     2941 acagttactt ctaaagatgc aaatacagta gttaatcaag cccttggtaa agtggttatt
     3001 actaaatatg atgatcgcag taactattca aatgtgggaa ataaagtctt agaaggtgtt
     3061 gaatttggat tgtaccaaga atctggtgac ttagttcaaa cagtaaaaac tactggtaaa
     3121 gatggtaaaa ttatatggac tgatgttcct gctggaaatt attatgtaaa agaagttgcg
     3181 actttagacg gttttgaact tgatactact gtgtatcctg ttactgttga atcaggacaa
     3241 agtacagtaa aagaatactt accaaataca gatggtaaaa taatcaacca ttctaatatg
     3301 ggtaaaatag ttgttaaaaa gacaagtgat gctaatgaaa acttagcagg ggcagtattt
     3361 aaaataacta atcctaacga tagtggtttt gtaccaatta ttattactac aaatgaagat
     3421 ggaatagcta aaacagaatt actgccagct aataaagaag gtacaactta tgtagttaca
     3481 gaaattaaag cgcctgatgg ttatactctt gatgacaagt atcatgacat cgaacaatct
     3541 gtaaaagtat atccagtcca agatgaaaca gttattttat cagaacagag taaaaactat
     3601 cttgaattta agaataaaaa gcaatctgat attatggata ttgatggtaa gatcataaag
     3661 tatattgatg gtgataataa cactttatta actgcaaaga ctgttgaaac accattatta
     3721 gatgctgcgg atacagaaac attcttattg agagaatatg ctcaagctaa aaatgatgta
     3781 ccagtaagaa gtgttgaagt ggaagatagt ttaattaaaa tgtattatta tgatgctaat
     3841 ggtaattcgg ttgaatatat gccagatgca ccttatgtag ttaatactgt aacagttcat
     3901 agtgcaactg ataaaaatgg taataagatt caagctcgag tatactatca aacagctggt
     3961 agtgatgaat ggacgactaa taatagttta gttgctgatg tctcaacgaa tagtcaagga
     4021 attagtttag aaggtttaaa tgcattaaaa atcaaagtag tatatacaaa tgttgttagc
     4081 aaggacttaa ttgcagagaa ttttaatgct acaggaattg atgtgaatgt gacatttaat
     4141 aaacgtcctt cagatgcgac tgttcatgaa gttagaagag tttcaaacca aacaagtgta
     4201 acatataact atacacttaa agataatgaa ggtaatgatc aaaatgcgca gtatacaaaa
     4261 gtttcagata tagtaaatct atattatcct ttaaaagaat cagtaactcc tcgagtttca
     4321 ctaggaattg tttctggtga gaaggataaa acttataagc caggtgaaac tattgaaagt
     4381 gtaattacgg ttaaaaatga atcaactaag gaaaatgaaa ttattgaaca accaattatt
     4441 agttttgatt tacctatcgg tatgtcacta aataataata gttatagtat aggagattta
     4501 caaactaggt tccaagtaat agtatttgaa catgatggag atacctcagg taaagcatta
     4561 gaaccagatg aatatactgt tacttatact aatgatgttc ctgctcgtgt tgttgaaaat
     4621 aatcaattag ttgaaacgac atcaaaaact aagaagataa caattgattt aggtgaagat
     4681 tttaaattta aacctggaat gaagatacag attagttata aaggaactag ttcaattaat
     4741 gatactagca caacattatg ggcaccagca tactttacta gtggaaaaat tattgctttg
     4801 tcagctgaaa atccttatgg aaatagtttt actgtcgaat ccgctacagg ttctagttac
     4861 aatacacttg ttgctgacag tgtgctagat gaaattacta ataaaccaga tgaaaccggt
     4921 gatggattaa agtatccaaa tagtaacgga atcattaaaa tcaatgaaca aaattattta
     4981 tcaatctata aacaagttaa aggtatttat gatgatgatt atttaaataa taatcagatt
     5041 gctaaaacag ctcctggcga agatttggat tataagattg tatttaaaaa tggtgaaaaa
     5101 tcagatcgtg ctgttagtaa ggcgcgtgtt gtagatattt taccatttga aggtgactcg
     5161 ttagttaatc gtacaaatga taactatact gcaagggtta caaatcttga taaatcacca
     5221 attttaaatt atgtagattg tttaacacca ggtgtttctt ataaagtcta ctattgtgtt
     5281 ggtgaaagtg aagatactaa atgggatgaa tggaaacaag atgcccgtac aagtaaaact
     5341 gcttcagaag aattgccaat tgtatatggt aatatggatg atgatgattg gacaagcggc
     5401 gctcatcaat ggatagaagc atcaaatgat attgatttga gactagttag tgctattgct
     5461 gttgaatttg atttctctaa tgcgccatta gaacctaatc agtcaattga attgcatgtc
     5521 aacatgtctg ctcctgagta cagtacttca gatttagaga aagtatctgg taagttaatg
     5581 tctaactctg ctttagttgc agttaaacgt actggtctag atactgtaaa tgacagtgat
     5641 attgttgaaa atcttgaagt taaagctgaa attgcattac ctaaaggaac aatcggtgat
     5701 tatgcattct atgatatgaa ccgtgatggt ttacaagata gcaatgattt accggttcaa
     5761 ggtttaaatg taacattgca taaatttgtt acaacgatta atcaaaatga aggaaaagta
     5821 actaaagaat tagattcaga aacaactttg actgatgcca gtggtaaata tgaatttaca
     5881 aatcaagatt gtaatgtttt aaaagatggt aagacggatt catcatcaac taatccaaat
     5941 gattatgttg gtaacaagta ttatcagtat caagttgaat ttgctattcc agaagatgag
     6001 tctttatata aatatgagcc aactcaaaga tacgctcaag atgttgatgg aaaactacaa
     6061 attgaagctg attctaatat tggaaatgat cttaataagg atgattattg taagacagaa
     6121 ttatttactt tgaccgcaac taaacaagct gatggcagct tagtaggaga aactaattta
     6181 acattagatg caggatacgt tgcattagga tctctaggag attttgtctg gtttgatgaa
     6241 aataaaaatg gtattcaaga tccagaagaa actggtataa aagatgtgat tgtaaatctt
     6301 tatacagtaa ctaatggtga ggttagtggt actccagaaa aaactactag aactgatgaa
     6361 aatggttact atttatttac tgatttagaa gatggaagtt atgttgttga atttgatatt
     6421 cgtggtgtta aaccaacaat gggtggtgga cacgcggaac gtttctactt tactaaaaat
     6481 ggtggaacaa cagatagtgc aattgattct aatccaacta ttactgatga aaatgagaat
     6541 actttaattg ctcgtagtga agtaattgat ttagcttatc atacaagtga tatgacaatt
     6601 gatgctgggt taacagttta tagtgcaata tctggtgttg catttgaaga tcgtgattat
     6661 tctgatattc aaaattatca agatagtgat ggtaaagatg ttgatatcaa attacctggt
     6721 acgattgttg aattatatcg ggttgatgaa gaatttggaa ttgaaaataa tatcccaaca
     6781 gaattagtcg catctactgt agttggtgaa gatggaagct atttatttga ttatttagat
     6841 tctggaacat atgttgttaa attcacatat cctgaaggat ataaggttat tgaagcagat
     6901 gttggtgatg atgatacgct tgatagtgat gtggcttatc atattgagac taatgagaat
     6961 aggatgtcgg gatatacagg agttattaat attcctgtca atacaagagt tgataatgtt
     7021 gatggtggtg caagattgta tagcagttta ggagactatg ttttcaaaga tgttaatgtt
     7081 gatggtcttc aagatgaaac agatatcgtg atgccggatg taccagtata tttgtttgct
     7141 agacaaaaag gtgaagaagt atggacatca attgcaaata caataactga tcaagatggt
     7201 agatatcgtt ttgataactt aaagggttca acttatactg gtattgaata tcgtgtaatc
     7261 tttgatttac cacttactac aaaattgaca gttccatatg ctggggatga tccagaacta
     7321 gattctaatg ctttaaatga atatgtacca ggacttggtt tcccaactca aactattgat
     7381 ttagcatata atacagtaga tttaacatgg gatgctggaa tcgttcaatc aaaaggttct
     7441 gtgggtgatt atgtttggta tgatacaaat gtcaatggca ttcaagatga aaatggaaca
     7501 ggtattgaag gtattaaagt tattttagaa actaatttaa caggtaatat tgctgatgaa
     7561 aatgattggc aagaagtagg tacaacttat acaaatagcc aaggatatta tattttcaat
     7621 gagctatcag aaggatatta tcgagttaaa tttgaaattc catcaaaata caaagttaca
     7681 ttgtctactc aaggtgaaga tagtgctgaa gatagtgatg gtatttatag cgaagatgga
     7741 gtttggtatt atacccgttc attctattta gatcaagatg gatatgatat gacatgggat
     7801 tgtggagtat atgacccaac agatacaaaa ttaactaaaa caggcagcac tgcaactggt
     7861 gatagttctg atataaatgg atatgttgtc ttaggattta gttcaatatt ggctttagga
     7921 gcagtatact ttgtatcaaa taaaaaaaga aaagctaaaa aagctgagaa aatctagcta
     7981 atttataaag aggcagatta aaacctgcct ctttgtatga taaattaaag ttttttgact
     8041 atttgctaat tttattgttt tttgtgata
//
LOCUS       DAOMTA010000029         8007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_109_length_8007_cov_2.743084, whole genome shotgun sequence.
ACCESSION   DAOMTA010000029 DAOMTA010000000
VERSION     DAOMTA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109_length_8007_cov_2.743084"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /gene="uvrB"
                     /locus_tag="PLK68_01560"
     CDS             <1..1691
                     /gene="uvrB"
                     /locus_tag="PLK68_01560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRM89127.1"
                     /translation="QPEAYIPGRDLYIDKNAKTNYEIEMLRSAAMNSLIEREDVIVVA
                     SVASIYGLGNPEQYKEMIFSLRVDQDIDRKELLTYLVDRQYQRNDIEQTKGTFRVRGD
                     VIEIVPGHTESWLIRIELFGDTVERISEVDPLTGHVLGVYNTYTIYPAYGYVTKKEQI
                     LKACDTITAELENRLETFKAETKLLEHERLEQRTRHDVEMLREVGMCPGIENYSRHID
                     GRDEGQRPYTLIDYFPKDFLMIVDESHVMLPQVRGMYNGDRSRKETLVEYGFRLPSAL
                     DNRPLRFEEFEKIINQVIYVSATPGDYELEHVENKVVEQIIRPTGLLDPVIEVRPTKD
                     QIDDIISEIKIRQDRNERVLITTLTKRMAEDLSAYLKELGIKVAYLHSDTKTLERTEI
                     LRDLRLGKYDVLVGINLLREGLDLPEVSLVCILDADKEGFLRSNRSLIQTIGRAARNS
                     NGEVIMYGDKITDSMAYAIEETNRRRKIQDAYNKEHNITPTTIHKEIRDAIRGQEVID
                     DAASLVKKGKKASKKDKQVMIQELEKQMKDAAKVLDFERAMELRDIIMELQGEK"
     gene            1681..3036
                     /locus_tag="PLK68_01565"
     CDS             1681..3036
                     /locus_tag="PLK68_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM89128.1"
                     /translation="MKNRYTFSPLAKKIFKDIDEHHGRAFLVGGIVRDMLIYNRVDYH
                     DVDVEVYGLSVDELEELLANYGNVNSIGKSFGILKLDVLPNFDFALPRTEIKTGESHQ
                     DFDVTVNQNLDLKVAASRRDLTINALMYEIKTGKIYDFFHGQEDIEKRTLRMVSETTF
                     IEDPLRVLRTAQFASRLDFCIETATKLMCKKMVQNKSLDKLSKERVFQEYSKLLLSQQ
                     PSIGLTFLKEIKALWPCLDVLSKTMQRLDYHPEGDVWRHTLLVTDLAALCCHKTSNPL
                     GFMWSALLHDIGKATVTTKDGHAPGHNEAGVKIFNQEVKAFIPDKQLQKYIKTMIFYH
                     MHLMNMVRNEAKDYSYFKILKGIDGIVTIEDLILITKCDKLGRYKDEHENINRFDYVM
                     KEKMARLSTKAQLPLVDGYDLKALGIEPSFKYSELLDWAYDLQLRGHSKAAILKMVEG
                     R"
     gene            3040..5862
                     /gene="uvrA"
                     /locus_tag="PLK68_01570"
     CDS             3040..5862
                     /gene="uvrA"
                     /locus_tag="PLK68_01570"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRM89129.1"
                     /translation="MEHDKLVIKGARENNLKNIDIEIPKNKLVIMTGLSGSGKTSLAF
                     DTIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIEGLSPAIAIDQKTTSNNPRSTVGT
                     VTEIFDYLRLLYARIGKAYCPEHGIVIESQTIKQMADTIDKYPDGSKLQVLARVVKNQ
                     KGTFKDLFEDLLKDGYIRVQVDGEVKLLDEGIELDKNKKHNIDVVIDRIIKKEGYRSR
                     LIDSLEVGLKLTGGEVVVANLSDGSEALFSEHLACPECGFSVPKLEPRLFSFNNPLGA
                     CPDCRGLGIKNEVDDDLLIPNWDLSINQGGIRYFKTSVGTDRIEWQRFLILCKTYKID
                     LDKPLKDFTKKELRIILEGSDKPISYEIVSSSGNVSKTNRPIEGVKTLIKRRFEETTS
                     SWSKEWYASFMAEHTCPTCGGRRLNDQVLSVRVGGLNISEFTDMSIEKALKFVDELKL
                     NEYEAKIANLVLKEIKDRLGFLNDVGLGYLTLSRKAGGLSGGEAQRIRLATQIGSRLT
                     GVLYVLDEPSIGLHQRDNDKLIATLQNIRDLGNSVLVVEHDEDTMRASDFIVDIGPGA
                     GVHGGEVIVAGTPQEVMDCKKSITGQYLSGRLKIDVPKKRRKGNGNFIQVKGAAANNL
                     KNINVKFPLGCMSVVTGVSGSGKSTLVNEIMGKAMMARIYRSKEKPGKHKDILGLENI
                     DKVIEVSQDPIGRTPRSNPVTYTGVFDDIRDLFAQTNEAKMRGYDKGRFSFNVKGGRC
                     EACQGDGLKRISMHFLPDVYVPCEQCGGKRYNEETLQVTYKDKNIYDVLEMTIEDAVE
                     FFGNLPKIKTKLQTLNDVGLGYIKLGQSATTLSGGEAQRVKLASELQKKATGKTVYIL
                     DEPTTGLHSNDVAKLIEVLQRIVDQGDTVIIIEHNLDVIKVADYIVDLGPEGGDGGGT
                     IVVAGTPEKVAKCEASYTGSFLKKML"
     gene            5876..6811
                     /gene="hprK"
                     /locus_tag="PLK68_01575"
     CDS             5876..6811
                     /gene="hprK"
                     /locus_tag="PLK68_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRM89130.1"
                     /translation="MPKSITVGELLNNEHYVQVTGDEKSLERPIYVAEINRPGFELAG
                     FFKHSDFRRIIIFGDKESAFINEMSEERQREIFPFLINDEVPCIIVAKGNECPAILIE
                     IARKKDFPIFITESATGRVSIELTNILDEALAPETLIHGVFLNIYGKGVIIKGDSGIG
                     KSEIALELVKRGHLLVADDAVELYHLGQSIVGKAPEVLTNLLEIRGIGVIDVSKMFGI
                     SSILDKDKVDLIIQLDRWVPSREYTRVGVEENDSTEEILGVKIPKIVVPVSSGRSMSV
                     IIEAAVMNMRLREYGVDSSKEFVDRILKNIDKNKG"
     gene            6815..7615
                     /gene="lgt"
                     /locus_tag="PLK68_01580"
     CDS             6815..7615
                     /gene="lgt"
                     /locus_tag="PLK68_01580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRM89131.1"
                     /translation="MTFFPDGKTFVQIGSFSIAWYAICIITGAFIAYKLGQYNFKKIG
                     YNKEILSDYFFGLMITGVLGARLWYVIFMWNELYAENPLEIIMFRHGGLAIQGGIFVG
                     LIFSWWYFKKHKIDFMVAADAIMPGVLIAQALGRWGNFFNQEAYGGMVSLDFLKSLHL
                     PNFIIEGMHINGYYYHPTFLYESIANIVGFLLIYFVIRKIQTKQGEQFFSYFIWYGIF
                     RFFIEGLRTDSLYVMGLRTAQLVSIVFVIVGIAGYIYCKKYGKPAIKA"
     gene            7813..>8007
                     /locus_tag="PLK68_01585"
     CDS             7813..>8007
                     /locus_tag="PLK68_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM89132.1"
                     /translation="MKLKYDVGDKPLWAQLFDILLDRIVSKEYNAGDKMPTDLEIMQE
                     FDVSRMTVRQAMNKLINEGYI"
BASE COUNT         2738 a         1018 c         1730 g         2521 t
ORIGIN      
        1 atcaaccgga agcttatatt cctgggcgag atttatatat tgataaaaat gctaaaacaa
       61 actatgaaat tgagatgctg cgcagtgcgg caatgaattc gttaatagaa cgggaagatg
      121 tcattgttgt tgcttcagtt gcttcaattt atggattagg aaatcctgaa cagtacaaag
      181 aaatgatctt ttcactgcgg gtcgatcaag acattgatcg taaagagtta ctaacatatt
      241 tagttgatcg gcaatatcaa cgcaatgata ttgaacagac taaaggaaca tttagagttc
      301 gtggggatgt cattgaaata gttccaggac atactgaaag ctggttaatt agaattgaat
      361 tatttggtga tacagttgaa agaatcagtg aagtggatcc tctgactggg catgtcttgg
      421 gcgtatacaa tacatatacg atctatccag cttatggata tgtaacgaaa aaagaacaaa
      481 ttttaaaggc ttgtgatacg attacggcag aattagagaa tcgtttagaa acattcaaag
      541 cagaaactaa attactagaa catgaacgtt tggaacaacg aacgcgccat gatgttgaga
      601 tgctgcggga agtggggatg tgtcctggaa ttgaaaacta ttcacggcat attgatggtc
      661 gtgatgaagg acaacgccct tatacgttga ttgattattt tcctaaagat tttttaatga
      721 tcgtcgatga aagtcacgtt atgttgcctc aggttcgagg aatgtataat ggggatcgtt
      781 cacgtaaaga gacattagtt gaatatggtt ttagattacc aagcgcgctt gataaccgcc
      841 cattacgctt cgaagagttt gaaaaaatta taaatcaagt catttatgta tcagcaacac
      901 ctggggatta tgagttagaa catgttgaaa ataaagtagt tgaacaaatt attaggccaa
      961 ctggactact tgatccggtt attgaagtac gaccaacaaa ggatcaaatt gatgatatta
     1021 tcagtgaaat caaaattcgt caagatcgta atgagagagt tctaataaca acattgacta
     1081 aacgaatggc tgaggattta agtgcttatt tgaaagaact tggaatcaag gtagcttatc
     1141 tacatagtga cacaaagact ttggaacgaa ccgagatact aagggatcta cgacttggca
     1201 agtatgatgt tttagtcgga attaatttat tacgagaagg tttagattta ccagaagtaa
     1261 gtttagtatg tattcttgat gctgataaag aagggttctt aagaagtaac cgttcattga
     1321 ttcaaacgat tggtcgggct gcccgtaata gtaatggtga agtaatcatg tatggtgata
     1381 agattactga ttcaatggct tatgcgattg aagaaactaa tcgtcgtcgt aagatccaag
     1441 atgcttataa taaagaacat aatattaccc caacaactat tcataaggaa attcgtgatg
     1501 caattcgtgg tcaagaggtc atcgatgatg ctgcaagtct agttaaaaaa ggtaagaaag
     1561 caagcaagaa agataaacaa gttatgattc aagaattaga aaaacaaatg aaagatgcag
     1621 cgaaggtact tgactttgaa cgtgcgatgg aacttcgaga tatcatcatg gagcttcaag
     1681 gtgaaaaata ggtatacttt cagtccactt gctaaaaaga tctttaagga tatagacgag
     1741 caccatggta gagcttttct agttggagga atcgttcggg atatgttgat ctacaatagg
     1801 gttgattatc atgatgtaga tgttgaggta tatgggctta gtgtcgatga attggaagaa
     1861 ttattagcta attatggcaa tgtcaatagt attggtaaat cattcggaat tttaaaatta
     1921 gatgttttac caaattttga ttttgctttg ccgagaacgg aaataaagac aggtgagagt
     1981 catcaggatt ttgatgttac agtaaatcaa aatttagatt taaaagttgc ggcatcacga
     2041 cgtgatctaa caatcaatgc gttgatgtat gaaataaaga caggaaaaat ctatgatttt
     2101 tttcatggtc aagaagatat tgaaaaaaga acattacgaa tggttagtga aacaactttt
     2161 attgaagatc cgttaagagt cttgagaaca gcacaatttg cttcacgtct tgatttctgt
     2221 attgagacgg cgactaaatt aatgtgtaaa aaaatggttc aaaataaaag tttggataaa
     2281 ctaagtaaag agcgggtatt tcaagaatat agtaagctcc tcttaagcca gcagccttca
     2341 attgggttaa cttttttaaa ggagatcaag gctttgtggc catgtttaga tgtattatcg
     2401 aaaactatgc aacggcttga ttaccaccct gaaggagatg tatggcggca tactttatta
     2461 gtaactgatt tagcggcttt atgctgccat aaaacaagta atccattagg ttttatgtgg
     2521 agtgctttat tacatgatat tggtaaagct acagtaacaa ctaaagacgg tcatgctccg
     2581 ggtcataatg aagcgggggt aaagattttt aatcaagaag taaaagcatt tatcccagat
     2641 aaacagttac aaaaatatat taaaacaatg attttctatc atatgcattt aatgaatatg
     2701 gtcagaaatg aagcaaagga ttatagctat tttaaaattc ttaaaggaat tgatggaata
     2761 gttacgattg aagacttaat tttaatcaca aaatgtgata aacttggtag atacaaagat
     2821 gaacatgaaa atattaatcg ctttgattat gttatgaaag aaaagatggc acggctatca
     2881 accaaagctc agttgccatt agttgacgga tatgatttaa aggcgttagg gattgaaccg
     2941 tcattcaagt atagcgagct attagattgg gcttatgatc ttcaattacg ggggcacagt
     3001 aaagcagcaa tcttaaagat ggtagaaggt aggtaaaata tggaacatga taaactagtc
     3061 attaaagggg ctagagagaa taacttaaaa aatattgata tcgagattcc aaaaaataaa
     3121 ttagttatta tgacgggatt atctggttca ggaaaaacat cgctggcatt tgatacaatc
     3181 tatgccgaag gacagcggcg ttatgtggaa tcgttaagtg cttatgcacg ccagttctta
     3241 ggaaacatgg aaaaaccgga tgttgatagt attgaaggtt tgtcaccggc aattgcaatt
     3301 gatcaaaaaa caacatcaaa taatccgcgt tcaacggtag ggacggttac tgaaattttt
     3361 gactatttaa gattacttta tgcgcgtatt ggtaaggcat actgtccaga gcatggaatc
     3421 gtgattgaat cgcagacaat caaacaaatg gctgatacaa ttgataaata tccagatggt
     3481 agtaaattac aagtattagc acgagtagta aagaatcaaa aaggtacttt taaggattta
     3541 tttgaggatt tattgaaaga tggttatatt cgagttcaag ttgatggtga agttaaacta
     3601 ttagatgaag gaattgaact tgataaaaat aagaaacata acattgatgt cgtaattgac
     3661 cgaattatca aaaaagaagg ttatcgtagt cgtttgattg attcacttga agtaggttta
     3721 aaactaacag gtggagaagt agttgtagct aatttaagtg atggcagtga agctttattt
     3781 tctgagcatt tagcttgtcc agagtgtggt tttagtgttc ctaaactaga accaagatta
     3841 ttctcattta ataatccact aggagcttgt ccggattgtc gcggtttggg aataaaaaat
     3901 gaagttgatg atgatttatt gattcctaat tgggatttga gcataaatca aggtggaatt
     3961 agatatttta aaacttcagt aggaactgac cgaattgaat ggcaacgctt tttgatccta
     4021 tgtaaaacat ataagattga tttagataaa ccattgaaag actttactaa aaaagaatta
     4081 agaattatct tagaaggctc agataaacca atttcttatg agattgtttc tagtagtggt
     4141 aatgtttcta aaactaatcg tcctattgaa ggtgttaaaa cattgattaa acgtcgtttt
     4201 gaagaaacga cttcttcttg gtcaaaagaa tggtatgctt catttatggc tgaacatacg
     4261 tgtccaactt gtggaggacg cagattaaat gatcaggttt taagcgtacg agtaggtggc
     4321 ttaaatatca gtgaatttac cgatatgtca attgaaaaag ctttaaaatt cgttgatgaa
     4381 ttaaaattaa atgaatatga agcgaagatt gctaatcttg ttttaaaaga aattaaagac
     4441 cgtttaggtt tcttaaatga tgttggatta ggatatttaa cattatctcg taaagcgggt
     4501 ggattatctg gtggagaagc acaaagaatt cgtttagcga cacaaattgg ttcaagatta
     4561 actggggttt tatatgttct agatgaaccc tctattggac tccatcagcg ggataatgat
     4621 aagttaattg ccactttaca aaatattcgt gatttaggta atagtgttct agttgttgaa
     4681 catgatgaag acacgatgcg agcaagtgat tttattgttg atattggacc gggtgcagga
     4741 gtgcatggtg gtgaagtaat tgtagctgga acgccacaag aagtaatgga ttgtaaaaag
     4801 tcaattacgg ggcagtattt atcaggtcgt ttaaagattg atgtgcctaa aaaacgccgt
     4861 aaaggaaatg gtaattttat tcaagtaaaa ggtgctgctg caaataactt aaagaatatc
     4921 aatgttaagt ttccattagg gtgtatgtct gtggttacag gggtttctgg tagtggtaaa
     4981 agtactcttg taaatgaaat catgggtaaa gcaatgatgg caagaattta tcgctcgaaa
     5041 gaaaagcccg gtaaacataa ggatatttta ggattagaaa atattgataa agttattgag
     5101 gtgagccaag atccaattgg gagaacacca cgttccaatc cggtaactta tacaggagtg
     5161 tttgatgata tccgtgattt atttgcccaa acaaatgagg ctaaaatgcg tggctacgat
     5221 aaaggacgtt ttagtttcaa tgttaaaggt ggacggtgtg aagcttgtca aggtgatggg
     5281 ttaaaacgta tttcaatgca ctttttacca gatgtttatg ttccttgtga acaatgtggc
     5341 ggtaaacgct ataatgaaga aacattgcaa gttacttata aagataagaa tatttatgat
     5401 gttttagaga tgactattga agatgcagta gagttctttg gaaacttgcc aaagatcaaa
     5461 actaaattac agactttaaa tgatgttgga ctaggctata ttaagcttgg tcaaagtgca
     5521 acaactttat ctggtgggga agctcagcgg gttaagctag caagtgaatt acaaaaaaaa
     5581 gcaactggga aaacagtgta tattttagat gaaccaacaa ctggtttaca tagcaatgat
     5641 gttgctaaat taatagaggt attacaaaga attgttgatc aaggggatac agtcattata
     5701 attgaacata atctcgacgt tattaaggta gctgattata ttgttgattt aggtcctgaa
     5761 ggtggcgatg gtggtggtac gatcgttgta gctggaacac cagaaaaagt cgctaaatgt
     5821 gaagcaagtt atactggaag ttttttaaag aagatgttat agggaggaaa caatgatgcc
     5881 aaagtcaatt acagtaggtg aattattaaa caatgagcat tatgttcagg taactggaga
     5941 tgagaaatct ttagagcggc caatttatgt ggctgagatc aatcgacctg gatttgaatt
     6001 agcaggattt tttaaacaca gcgattttcg acgaattata atttttggag ataaagagtc
     6061 tgcatttatt aatgagatgt cggaagaaag acaacgtgaa atattccctt tcttaattaa
     6121 cgatgaagta ccatgtatca ttgttgctaa aggaaatgaa tgtccagcaa tcttaattga
     6181 gattgctcga aaaaaagatt ttccaatttt tattaccgaa tcagcaacag gacgtgtttc
     6241 aattgaatta acaaatattc ttgacgaagc actggcaccg gaaacactga ttcatggagt
     6301 ctttttgaat atttacggta aaggtgttat tatcaaaggt gatagtggta tcggtaaatc
     6361 tgagattgct ctagaattag taaaaagagg acatttatta gttgctgatg atgctgttga
     6421 attatatcat ttaggacaaa gtattgtggg gaaagcccca gaagtattaa ctaatttatt
     6481 agaaattcgt ggaattggtg ttattgatgt atcaaagatg tttggaatta gctcaattct
     6541 cgataaagat aaagttgatt taattattca attagatcgt tgggtaccat ctcgtgaata
     6601 tacacgagta ggggttgaag aaaatgattc aactgaagaa atcttaggtg ttaagattcc
     6661 taaaattgtt gtacctgtat caagtggacg tagtatgtca gtaattatcg aagctgccgt
     6721 aatgaatatg cgtttacgtg aatacggtgt tgattctagc aaagaattcg ttgatcgtat
     6781 tttgaagaat atcgataaga ataaggggta agttatgact ttttttccag atggtaagac
     6841 attcgttcaa atcggttctt tttccattgc ttggtatgca atttgtatta ttaccggagc
     6901 ttttattgca tataaactag gacaatataa tttcaaaaaa atcggttata ataaagagat
     6961 tctttcagac tatttctttg gtttaatgat tactggagtt ttaggagctc gattatggta
     7021 tgtaatcttt atgtggaacg aattatatgc tgaaaatcca cttgagatca ttatgtttcg
     7081 tcatggtggt ttagcgattc aaggtgggat ttttgttggc ttgattttta gctggtggta
     7141 ttttaagaaa cataaaattg atttcatggt tgcagctgat gcaattatgc caggtgtttt
     7201 aattgctcag gcactgggac ggtggggaaa ctttttcaac caggaagcat atggtggaat
     7261 ggttagtcta gatttcctta aatcattgca tttaccaaac tttattattg aaggaatgca
     7321 tatcaatgga tattactatc atccaacctt tttatatgag tctattgcta atatcgtagg
     7381 atttttatta atctattttg tgattaggaa gatccaaaca aaacaaggtg aacaattctt
     7441 tagttatttt atttggtatg gaatctttag attctttatt gaaggacttc gtactgatag
     7501 cttatatgta atgggtctac ggacggcaca attagtttcc attgtttttg taatcgttgg
     7561 aattgcagga tatatatatt gtaaaaaata tggtaaaccg gcgataaaag cgtaatctaa
     7621 attaggtagt taaagtgaat agcactgagc tgcctttttt ttaatttaat aaagtgtgtc
     7681 tatttctatt agttataaca tagatatatt atgtttttat tactatttat agaaactgaa
     7741 ttgtttctct agtataatat aggggaaatt agtaatttaa atagatttta ttataggtgg
     7801 gaggaaaaca aaatgaaatt aaaatatgat gttggagata aaccattatg ggctcaatta
     7861 ttcgatattc ttttagatag aattgtttca aaagaatata atgctggtga taaaatgcca
     7921 acagatctag aaataatgca ggaatttgat gtttctcgaa tgactgttcg tcaagctatg
     7981 aataaattga ttaatgaagg ctatata
//
LOCUS       DAOMTA010000030         7661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_116_length_7661_cov_3.084407, whole genome shotgun sequence.
ACCESSION   DAOMTA010000030 DAOMTA010000000
VERSION     DAOMTA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_7661_cov_3.084407"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2527)
                     /locus_tag="PLK68_01590"
     CDS             complement(<1..2527)
                     /locus_tag="PLK68_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM89133.1"
                     /translation="MKKILKRLATGFLAFATIVTALPTTAVHASEKQYWTESAERVGI
                     VEKVMNDGSIGSTFNEGHMTVEGEDAFCVDINTNFRNGYKTRVDASTRMSADQISDVA
                     LSIEYVNQYAQSHSGLSSQHIYLLKQCVVWQRLSVHLGWQCDNVRASYDEISKAVQDE
                     VYAGAKAFASENKGRYECGGYIYSGEGQDIGQFWAKLAVGNATLKKTSSNTSITDGNG
                     LYSIAGATYGVYSDKDCTKQLATLTTDNSGNTDTVEVKAGTVYIKELSAPAGYKVDTT
                     VYSLNVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDAPQGDASLEGAEFTWKFYA
                     GHYTADNLPSEPTKTWVTKTIAEKDSDGTIHYVSRLSDEYKVSGDSFYTQDGKNVLPL
                     GTLTVEETKAPNGYLLDGAYMQANGNEEQIKGMYLTQITEDGDIAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETSDTKGQGSATLKDAEFEIISLNGNSVLVEGKLYSKNEVVKTIL
                     TDIEGVASTSADLLPYGKYRIEESKAPEGYLTEGAKPIEFEITEDGKIVDLTGTDTSI
                     YNQVKRGDLEGVKIGAGTHQRLAGVPFRITSKTTGESHIIVTDDNGQFSTSSDWASHK
                     NNTNAGKTSEDGIWFGTSEPDDSKGALIYDTYIIEELRCESNKGFELIPAFEIVVSRN
                     NVTVDLGTLTDEYEKEIYIHTTATGKDGEKSIVAGKEVTIVDTVTLDGLEKGTKYQLK
                     GWQMLKEENAELLIDGQRVESDYTFTADSEEMKIEIEYTFNASSLGGQNLVTFEELYD
                     LSNEDEPVKVAEHKDIDDEEQTVLITERIISIHTTATSEDGKKE"
     gene            complement(2816..3883)
                     /locus_tag="PLK68_01595"
     CDS             complement(2816..3883)
                     /locus_tag="PLK68_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM89134.1"
                     /translation="MSYFDLIINITEAFICTLFVYKLFNRKFSTYLIASLFYSGVITA
                     CNFTSSPEIIMIVLSILILFSYSHFLNKANYLQNIFIALFINTLVNVATTLSICFSTL
                     LAKFPIDSVDGYHLMAMISKPILLVLVLATASHIKKYYFLESQVLKYITISIVLLNFI
                     YSVTVDYIFYGDNLNSSLTLLLLLIDALTICLCFVFFEAQREQYRILELQRKSMKIEN
                     LETIQSINENNYQQLRNWKHDIIHVFNAIKYQLTNKNYNEAFKIINEYNHNLNSNNFV
                     QSSGNELLDYLLLQKVNQIKDKEIHLITSCHNTIGPLEDAHFFIIVGNLIDNAIENCD
                     SNFNKQLWVSIGTKPEYYFIM"
     gene            complement(3880..>4411)
                     /locus_tag="PLK68_01600"
     CDS             complement(3880..>4411)
                     /locus_tag="PLK68_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM89135.1"
                     /translation="PKEDGFSFAERLNNYYETKIIVVTNNNDYRANAYNIHPFQFINK
                     QNIKIEIINVCNQLFKSLKKNDKFITGYIYDRKKNIRIKDIYYIIVEDHLCYIFLYEN
                     KKSICIRDTIGNIEKSHLSNGLVRINRSTIINMLHIKDIKKNKICLKNNIELIISLGY
                     NKHFEKHYQSYIWGLL"
     assembly_gap    4412..4441
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4724..5296
                     /locus_tag="PLK68_01605"
     CDS             4724..5296
                     /locus_tag="PLK68_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HRM89136.1"
                     /translation="MIDSLSNKLVNYLDKGNYLDEDKEIYLHGAKLIISEVIGTLLLL
                     FLGLMTNHFIEAVVYEIVLSSTRSILGGYHCKSYAACICTYAGFFLAGVIFLNYYHFT
                     LMTILIVCLIGSINILALGPVDNVNKVASKKKKDVFKRYSKIVLIIYLTIIAALFHIH
                     NGYLDIMVYIFIVINLLMLGGKIDYEKSKK"
     gene            5277..5420
                     /locus_tag="PLK68_01610"
     CDS             5277..5420
                     /locus_tag="PLK68_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HRM89137.1"
                     /translation="MKNLKSKVLKVLSSGLFLVAVQAVSVYSQRHIYQEQEPEALKKY
                     DRY"
     gene            5859..6005
                     /locus_tag="PLK68_01615"
     CDS             5859..6005
                     /locus_tag="PLK68_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89138.1"
                     /translation="MDSERNGGNGNMKTDNALLEQRLSDTNLGLAFTQVKRNKSASGA
                     DGSR"
     gene            5992..6789
                     /locus_tag="PLK68_01620"
                     /pseudo
     CDS             5992..6789
                     /locus_tag="PLK68_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
     gene            complement(7227..>7661)
                     /locus_tag="PLK68_01625"
     CDS             complement(7227..>7661)
                     /locus_tag="PLK68_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM89139.1"
                     /translation="KVSNQLIFSPTLTFVERKDCLVGLQETYPLQFKEVIPIDGSPRY
                     TALVNNECDLVDAFSTDGLLKKFDLKVLTDDKNFFLPYNAIPIINQRIKDECPEIIEL
                     VNQLQNYLNEEVMVDLNYKVDEEKQKTKDVAYNFLLENNLIK"
BASE COUNT         2399 a         1278 c         1478 g         2476 t
ORIGIN      
        1 tctctttctt tccgtcctcg ctggtcgctg tggtatggat agagatgata cgctctgtga
       61 taagcaccgt ctgttcctcg tcgtcaatgt ccttgtgttc agccactttt acaggctcgt
      121 cctcattgga aaggtcgtaa agttcctcaa aggtaacaag gttctgaccg cccagagaag
      181 aagcattgaa cgtgtattct atttcaatct tcatttcctc gctgtcggct gtgaatgtgt
      241 agtcactttc cacacgttga ccgtcaataa gaagctcggc gttttcttct ttcaacatct
      301 gccagccttt caactgatac tttgtgcctt tttccagccc gtcaagcgta accgtgtcaa
      361 cgattgttac ctctttgcca gccacgatag acttctcgcc gtccttgcct gtggctgtgg
      421 tatggatata gatttctttc tcgtattcat cagtcagtgt ccctaagtca acggtcacat
      481 tgtttctgga caccacgatt tcaaaggcag ggataagctc aaagcctttg ttgctctcac
      541 agcgtaattc ctcaatgata taggtatcgt aaatcaaagc acctttgctg tcgtctggct
      601 cgcttgttcc aaaccagata ccgtcctcgc tggtctttcc tgcgttggtg ttgttcttat
      661 gggaagccca gtcggaagaa gtggagaact gcccgttgtc atcagttacg ataatatggc
      721 tctcacctgt ggtcttgctg gtgatcctaa agggaactcc cgcaagacgc tggtgtgtac
      781 cagccccaat cttgacaccc tctaggtcgc cacgcttcac ttgattgtag attgatgtgt
      841 ctgtccctgt aaggtcaacg atttttccgt cctctgtgat ttcaaattca atcggtttcg
      901 ccccctctgt tagatagccc tctggagctt tgctttcctc aatacggtat tttccgtaag
      961 ggagcaggtc agcagaagta gaagctacgc cctcaatgtc ggtaagaatt gtctttacca
     1021 cttcattctt gctgtaaagt ttcccctcga caagtacaga gttgccatta agggaaatga
     1081 tttcaaattc agcgtctttc aaggtcgcac ttccttgacc tttggtatcg ctggtttcca
     1141 aatctctttt ctggattttc acaccgccac ggataacctt gtctgataca tggtactgat
     1201 tgcttccaga cagtacggca atgtcgccgt cctcggtaat ctgtgtaagg tacattccct
     1261 taatctgttc ctcgttaccg tttgcctgca tatatgcacc gtcaagaaga tagccgttag
     1321 gagctttcgt ttcctcaaca gtcagcgttc ctaaaggaag cacgttcttt ccgtcctgtg
     1381 tatagaagct gtccccagat accttgtatt catcagaaag gcgggaaaca taatggatag
     1441 tgccgtcgct gtccttttca gcgattgtct ttgtcaccca tgtctttgta ggctcgctag
     1501 ggagattgtc agcggtatag tgtcccgcat agaatttcca tgtgaactcc gcaccctcta
     1561 gggaagcgtc gccctgtgga gcgtctttct gtgtttccat gtcaatcttg aataattcaa
     1621 tcaaggtgtc tgtaaccttt ggcgtatctg atacgttcaa ggtcgctgtc ttgccagctt
     1681 ccacatttaa ggaatacacc gtggtatcta ccttgtagcc agcaggagca gaaagctctt
     1741 tgatatagac tgtgccagcc tttacttcca cggtatccgt atttccgctg ttatccgttg
     1801 taagtgtggc aagctgtttc gtacagtcct tatcagaata gacaccataa gtcgcaccag
     1861 cgatagagta aagcccgttc ccgtcggtaa tgctggtatt actggaagtc tttttcaatg
     1921 tggcgtttcc tacggcaagt ttcgcccaga actgtccaat atcctgcccc tcgccagagt
     1981 agatataacc gccacattca tagcgtccct tgttctcgct ggcaaaggct tttgccccag
     2041 cgtacacctc gtcctgtacg gcttttgaga tttcatcata ggaagctctg acgttatcgc
     2101 attgccagcc cagatgaacg ctcaatctct gccatacgac gcactgtttc aaaaggtaga
     2161 tatgctgact gctcaatccg ctgtggctct gtgcgtactg attgacgtat tcaatcgaga
     2221 gggcaacgtc agaaatctgg tcggcactca tacgggtgct tgcgtcaact ctggtcttat
     2281 agccattgcg gaagttggtg ttaatatcca cgcaaaaagc gtcctcaccc tcaacggtca
     2341 tgtgtccctc attgaacgtc gaaccgatag agccgtcatt cattactttt tcgacaatgc
     2401 caacacgctc ggcactttct gtccagtatt gcttctcgga agcgtggact gccgttgtcg
     2461 gcaaagcggt aacgatagtc gcaaaggcga ggaagcccgt ggctaatcgt tttaatatct
     2521 ttttcatagc ttttaaaatt ctcctttcgt ttggatatag aaaaagccgt tcatttctga
     2581 acggctggaa atagaaaagg aacgtcaata tcggcgttcc ataatctact tgcgatattc
     2641 aattttaatc ttttcaatac tgatgtgctg taccatatct ttgcatatct gggatttcta
     2701 acgtatcaag gctcattcaa tggtagtaaa atcgtagtaa tttaaagtat ttcaatcctt
     2761 gaatgtgctg ataagtacag tgttttagcg gacaatcaga tttttagttg tttttttaca
     2821 taataaaata atactctggt tttgtcccga tagaaaccca aagttgttta ttgaaatttg
     2881 aatcgcaatt ttcaattgcg ttgtcaatta agtttcccac aataataaag aaatgggcat
     2941 cttccagtgg accaatggta ttatgacaac tagttataag atgaatctct ttatccttta
     3001 tttgattaac tttttgtaat aataaataat ctaataattc atttccagaa gattgaacaa
     3061 agttattgct atttaaatta tgattatatt cattgatgat tttaaaagct tcattataat
     3121 ttttattagt tagttggtat tttatggcat taaagacatg gataatatca tgtttccaat
     3181 ttcttagttg ttgataatta ttttcattga tactttgaat agtttctaag ttttcaattt
     3241 tcatactttt tcgttgtaat tcgagaatac gatattgttc gcgctgggcc tcaaagaata
     3301 caaaacaaag acagattgtt agggcatcaa taagcaataa tagcaatgtt agagaggaat
     3361 taagattatc cccataaaat atgtaatcaa cagtaacgct gtaaataaag tttaataaaa
     3421 caattgaaat tgtaatatat ttaagtactt gactttctaa aaaataatat tttttaatat
     3481 gactggctgt tgcaagtacg agaacaagta agattggttt tgaaatcata gccattaaat
     3541 gataaccgtc aactgaatcg attggaaatt tggctagtaa agtagagaaa caaattgata
     3601 aagtagttgc tacattaaca agagtattaa tgaacaaagc gataaagata ttttgtaaat
     3661 aattcgcttt atttagaaag tgtgaatagg aaaagagaat tagaattgaa agaacaatca
     3721 ttattatttc gggtgatgat gtaaaattac atgctgttat gactcccgaa taaaagagag
     3781 aggcaatcaa ataggtggaa aatttcctgt taaataattt gtatacaaat aatgtgcaaa
     3841 taaaagcttc agtaatattt ataattaaat caaaatagct catagtaacc cccaaatata
     3901 gctttgatag tgtttttcga aatgtttatt atatcctaag gatatgatta gttcgatatt
     3961 attttttaaa caaattttat tctttttaat atcttttata tgtaacatat ttattatagt
     4021 agaacgatta atgcgtacta atccattaga aaggtgggat ttttcaatgt ttcctatagt
     4081 gtcacgaata caaatagatt ttttgttttc atataaaaag atataacata agtgatcttc
     4141 tacaatgatg taataaatat ctttgatgcg aatatttttc ttccgatcgt aaatataacc
     4201 agtaataaat ttatcattct ttttcaatga tttaaataac tgattacaga cattgattat
     4261 ttctatttta atattttgtt tattaataaa ctgaaatgga tgaatattgt atgcgtttgc
     4321 gcgataatca ttattattag taacaactat aatttttgtt tcataataat tattaagacg
     4381 ttcggcaaat gaaaaaccat cttctttagg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nagatagcgt cataaaaaat actcaaatca aaattcaatg aattattata aacatcaagt
     4501 ttatattcaa tattatgaag ctgacattcc ttttctaatg tcgataggat taatttttgg
     4561 tcaaaatgat tatcatcaat aattgcaaac ttatacatag ttatttctcc tttagtgact
     4621 attatatacg aaacttgacg attcgcgaaa taaaatagta ctttacgaca tatatattgt
     4681 attaatttgg tgttatgata ttgtgatgaa aggagatgtg gttatgattg atagtttatc
     4741 aaataagtta gtgaattatt tagacaaagg taattattta gatgaggata aagaaatata
     4801 ccttcatgga gctaagctga ttatttctga agtgattggg acgttgctgc ttttattttt
     4861 gggtttaatg actaatcatt ttattgaagc agttgtttat gaaattgttt tatcaagcac
     4921 aagatcaatt ttaggcgggt atcattgtaa aagttatgcc gcttgtattt gtacatatgc
     4981 tggttttttc ttggcgggtg ttatattttt aaattattat cattttacat taatgacaat
     5041 attaatagtg tgtttgattg gttcaattaa tattttagct ttaggtccag tagataacgt
     5101 gaataaagtt gcgagtaaaa agaagaaaga tgtattcaaa cgttattcaa aaatagtctt
     5161 aataatttat ctaacaataa tagcggcatt attccatatt cataatggat atttagacat
     5221 aatggtttat atatttattg tgattaatct actaatgttg ggagggaaga ttgattatga
     5281 aaaatctaaa aagtaaagta ttaaaagttt taagttcagg tttattttta gttgctgttc
     5341 aagcggtaag tgtatattca caaagacata tttatcaaga acaagaacct gaggcattaa
     5401 agaaatacga cagatattaa taagaaaagg attgagataa attgcgtatc tcaatccttt
     5461 tttgtatgcc gggcatggca tatatctagg tggtgaaagt ccactgtggg ggtgccaacc
     5521 actagccaat atcaagggtg tctatcgtga ggtggaatct gaaagaagat ggaggcaaaa
     5581 tcttggtccg aggaacatga atcacatcag gcatataatg gggacgagtg tgcaaagcaa
     5641 catgaagtca taagaggttg tggaatgaaa ctattatatg taaatgtggc agatatatgg
     5701 aaggaaagag atatgacctt atcctgggag gtctcatgat ggagaaatcc tagtaataat
     5761 gaatcatggg aagtcagcag aggtcgtagt agcaaagaag tggctgtaat ggtcatggag
     5821 cgaaggcccg aaagaagaat acaggggaag tgagtataat ggatagcgaa agaaatggag
     5881 gtaacggaaa tatgaaaact gataatgcat tgcttgaaca gaggcttagt gatactaatc
     5941 taggactagc atttacacaa gtaaaacgaa acaaaagtgc aagtggagca gatgggagta
     6001 gatgaactta aaggttatct agaagaaatt aaggacagta tacgtaacaa gacatataag
     6061 ccagaacttg tacgaagagt agaaatacct aaacctgatg gtggagtaca aaatttagga
     6121 gtaccaacag tacttgatag atttgttcaa caagccatag cccaagtatt aatacctatc
     6181 tacgaatcaa tatttagtga caatagtttt ggctttagac caaataggtg ttgcgaaatg
     6241 gcaattatca aagcgttaga atatatgaat taaggctttc agtaggtagt ggatatagac
     6301 ttggaaaagt tctttgatac atttaatcac gataaactca tctcacttgt catgaagggt
     6361 gtaaaaagtg aggagatagt atctctaatt agaaaattcc tagtgagtgg aattatgatt
     6421 gataatgaat atagagagtc agtcgtagga acaccacaag ggggaaatct aagtccccta
     6481 ctcagtaaca tagttctcaa tgaactagat aaagaaatgg aagcacgagg gctaagattc
     6541 acacgttatg cagacgattg tattatatta gtgggtagca gtaaagccgc cgatagagta
     6601 atggaaaaca ttagtaaatt catagaaaag aaacgtagat ttaaggtaaa catgactaaa
     6661 agcaaaattt tgaaacctaa tgatattaaa tatctaggat ttggtttcta ctatgactct
     6721 ttttcatcta tgtggaaagc aaaaccacat gagaaaactg caaacaaaat tgaagaaact
     6781 aacaaataga agttggtcag tatcttggga atatagaata cttaagataa gacaactgat
     6841 agttggatgg attaattact attggatagg aaattttatg ggtgtatgta gaaaattaga
     6901 taaacaaata aaatttcgaa taagaatgtg cctttggaag aagtggaaga cgaacaaaag
     6961 cagggaaaag caactaataa aattaggagt attaccatgg caagcaaaga cttaggcaaa
     7021 ctgtcgtgag tcatatgcaa gatgtgcaag cacatttcta caaacaaaaa tcacaaacaa
     7081 tttactatat aaaaaagggc taccatcaat ggtagtccag tatcagttaa aacatatttc
     7141 aatatagatt taaaccgtcg tacggcacgt acggtggtgt gagaggtcga aaataaatat
     7201 aaaattattt attttctcct actcgattat tttattaaat tgttttctaa taaaaagtta
     7261 taagcaacat cttttgtttt ttgtttttct tcatctactt tataatttag gtctaccatt
     7321 acttcttcgt ttagataatt ttgtaattga ttaactaatt caatgatttc tggacattca
     7381 tctttgattc tttgattaat gatcggaata gcattgtatg gtaagaagaa gtttttgtca
     7441 tcagttaaca cctttaagtc aaatttcttt aatagtccat cagtagaaaa agcatcaact
     7501 agatcacatt cattattaac taatgctgta tatcgaggtg aaccgtcaat tgggataact
     7561 tctttaaact gaagtggata tgtttcttgt aaaccgacta agcagtcttt tctttcaaca
     7621 aatgttaatg ttggtgaaaa gattaattga ttagaaactt t
//
LOCUS       DAOMTA010000031         7567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_121_length_7567_cov_1.985889, whole genome shotgun sequence.
ACCESSION   DAOMTA010000031 DAOMTA010000000
VERSION     DAOMTA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_7567_cov_1.985889"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="PLK68_01630"
     CDS             <1..1801
                     /locus_tag="PLK68_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HRM89140.1"
                     /translation="YIISKPIKKLSDEVVKANIKNKISFRRTNIAEIDHLANMMEQLN
                     QNVRDTALKFTNLLQMASIKLAGFEYNINTEELFISENFFEVLLKYDVNTSELTMTKF
                     KETFEGYKQYIVSHDYSKKEYLFKIPDDDNYVFVNLRLLVNDNAYTGVIENVTNTIVE
                     KNVIEYERDHDALTGLLNRRAFIRIMNSLFENEINKIKIAALLMLDLDNLKYINDNYG
                     HEIGDNYISKAAETFVNSTPENTIISRISGDEFFVFFYGYDDENVIKQLINKLKEAIS
                     KAFIPLADNSNFHVNASGGIAWYPRDSESFEKLQHYADYAMYKIKRTSKGNLTEFNLN
                     DYIADSFLSESKEELNTIIEDRAIQFYFQPIISSKDGTIYAYESLMRSFMPSLKNPLD
                     ILKIAKEEGRLAEIEELTWSSSLETFANHLRNERINKDCKIFINSISNQILSNKKIDE
                     LEKEYSKYLKNVVLEVTEVEYIDENYHQQKAELMKKWQAELALDDYGSGYNSDRILLL
                     ISPKFIKIDMDIIRNIDSDPDKCKIVENIVNYAHERDMKLIAEGIETIDELKQVIKLK
                     VDYLQGFLLAKPQYLPPRIQEDVIKLIQLLNEK"
     gene            complement(1908..4238)
                     /locus_tag="PLK68_01635"
     CDS             complement(1908..4238)
                     /locus_tag="PLK68_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM89141.1"
                     /translation="MGKIKGLNKQEVAKRISEGKVNLSPKPVVKSNFQIIMGHVFNLF
                     NAYNFIIAAALIAVQAYSSLFFVVIVISNTVIRARQEIKSKNMVAKLNLIVSPKTKVI
                     REGKTISIDNEEIVLDDVVYFETGNQISADSIIIENNVEVDESLLTGEADPISKGPGD
                     HLLSGSFILSGACYGKVEHVGKDNYANKITDQARTRKPVSSVLLNTFNKVTRYTSYLV
                     LPLSILMLYQAYIIRDQGITSTIVNTATALLGMLPKGLVLLTSVSLAASVVKLGKMNT
                     LVQEMFSIETLSRIDVLCLDKTGTLTEGKMEIEQVIKLKNPLNLDLDEIMCSFVKGSL
                     DNNITFKTLSNYFTGPAKYETLDRIAFSSARKWSAIELDSIGTIIVGAPEIIRPDYQL
                     SPRIIEMQKSGARVLLIGHHPTLNILQETLGQTTPIGLIVIKDPIRKDAHEALKFFSD
                     NDVAVKVISGDNPATVSAIASQAGVANAEKYIDASTIVTDQQLEDAILNYNVIGRASP
                     FQKHQMILCLQKHNQKVAMTGDGVNDVLALKDADVSIAMGSGSDIARQVSQFIIIDGK
                     LQTLVEVVREGRLVINNVTRSASMYYLKTIYTILLSILSILMNIPYPFIPFQMTLLDM
                     FIEGFPSFMILFERNIEKPKESIGHHAMRFSLPNALAIVLSVAGIRLLAPTLQLSLAE
                     TFSVLYFTTAFVSIHMIYRIYKPLNWYRGGVLIIDIIGFILSTPIFWPLLEMHVLTPK
                     LIQIILITIVISIPVLIILTRSVSYYLKNLNSKKAL"
     gene            complement(4480..5907)
                     /locus_tag="PLK68_01640"
     CDS             complement(4480..5907)
                     /locus_tag="PLK68_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM89142.1"
                     /translation="MKLFNKFSKKQKITFISVLFILIISTVYLIWQKNNQLLTFVKDP
                     ILEINHEFDYQKIIKNIKNVQTSDIKVDTSKLKNDKIGSYPVTYIYQGKKFTVNINVV
                     DKTKPKFEINNIEIDAGMDVDPTMLVDNIVDETKTTISFKDAYNFSKKGKVKVTVIVS
                     DESNNKSTKHATVTVLSKDTTKPTISGLNDLTVTLNGKVDYLAGVKGNDNRDPHPQLT
                     IDNKKVRLDKLGDYIVTYTCKDRSGNKIVAKRKVSVVEKKEIGVYAQTEEKVIYLTFD
                     DGPSKNTKRILDILDKYHAKATFFVTGTNQNYNYLIKEAYQRGHTIGLHTYSPDYKTV
                     YTSVTAYFNDLERVGNMVKNEIGFIPKYIRFPGGASNTISRKYCPGIMSVLANEVIDR
                     GYQFYDWNYGTGDAGGNNIPVNQIIATATAGNANNQVILAHDTDAKNTTVDALPAIIE
                     HYQALGYSFKSIDDNSYVPHHHINN"
     assembly_gap    5933..5994
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6133..7311
                     /locus_tag="PLK68_01645"
     CDS             6133..7311
                     /locus_tag="PLK68_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HRM89143.1"
                     /translation="MRIDPFNDIDGLILSELVYVDFDKIVPTFPNEQSYCLKDVANNF
                     SQVNNIEELLDEISFTKESITLLQELAKCPRYQNILLSNYINELDYQSIKQFAAITFQ
                     LEDSSIYVAFRGTDDTILGWREDFQMTYQFPVASQQRAVEYLAKIAEIDFSKSLFYLL
                     CHCDYKMKWYQIFRIYITQKISGVKIRVGGHSKGGNLAVYASSCVNSKISKRIIEIYN
                     NDGPGFNQKMLNTDNYRNISTRIKKFIPESSVFGIMLENLEEKIVVKSSAKGLMQHSG
                     FSWSVEGTQFVCATEMSKDSQTMDAAFKSWLSKVDSKTRKDAVTAVFSILEAAKIEKI
                     NDFSEKSLSHLITAIKEIKNMNSDTRNALIQFFKTLIVETYDCSRKEHKKKDTNENNS
                     "
     gene            7365..>7567
                     /locus_tag="PLK68_01650"
     CDS             7365..>7567
                     /locus_tag="PLK68_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89144.1"
                     /translation="MNKFQNALYRFMSGRYGSDQLNNFLLIFALILLILNLFVIRNPY
                     LATIIWIILIINIFRTYSRNIYK"
BASE COUNT         2709 a         1059 c         1151 g         2586 t
ORIGIN      
        1 ttatatcatt tcaaaaccaa taaaaaaatt atcagatgaa gtcgtaaaag ctaatattaa
       61 aaataagatt tctttcagac gtacaaatat tgctgaaatt gatcatcttg caaatatgat
      121 ggaacaacta aatcaaaatg ttcgtgatac tgccttgaaa ttcactaatt tattacaaat
      181 ggcaagtatt aagctagcag ggttcgaata taatataaac actgaagaat tgtttatatc
      241 agaaaacttt tttgaggttt tactaaaata tgatgttaat acttcagaat taacaatgac
      301 aaaatttaaa gaaacatttg aaggctataa acaatatatt gtttcacatg attatagcaa
      361 aaaagaatat ctcttcaaaa ttcctgatga tgataattat gtttttgtga atctacggct
      421 attggtaaat gataatgcat atactggggt aattgagaat gtaactaata caattgttga
      481 aaagaatgtg attgaatatg aacgtgatca tgatgctcta actggacttt tgaatcggag
      541 agcatttatt aggatcatga atagtttatt tgaaaatgaa ataaacaaaa taaaaatagc
      601 agcactattg atgttagatt tagataatct gaagtacatt aatgataatt atgggcatga
      661 aattggtgat aattacattt ctaaagctgc tgaaacattt gtaaacagca ccccagaaaa
      721 tactattatt tcacggattt ctggtgatga attttttgtt ttcttttatg gttatgatga
      781 tgaaaatgta attaagcagt taattaataa attaaaggaa gcaattagca aggcttttat
      841 tcctttggct gataatagta attttcatgt caatgcatca ggcggtattg cctggtatcc
      901 acgggatagt gaatcttttg aaaaactcca acattatgct gattatgcaa tgtataagat
      961 caaacgtaca agtaaaggta atttaacaga gtttaatctc aatgattata ttgcggattc
     1021 ttttttaagc gaaagtaaag aagaattaaa cactattatt gaagatcgag caattcagtt
     1081 ttattttcaa ccaattataa gtagcaaaga tggaactatt tatgcatatg aatcattaat
     1141 gagatcattt atgccatcat taaaaaatcc ccttgatatt ttaaaaatag ctaaagagga
     1201 gggacgttta gcagaaattg aagaattaac atggtcctca tcacttgaaa cctttgccaa
     1261 ccatttacga aatgagcgaa ttaataaaga ttgtaaaata tttattaatt ctatttcaaa
     1321 tcaaatttta agtaataaaa aaattgatga gctagaaaag gagtattcaa agtatttaaa
     1381 aaatgttgtt ttagaagtta ctgaagttga gtacattgac gaaaattatc atcagcaaaa
     1441 agcagaatta atgaaaaaat ggcaagcaga attagcatta gatgattatg gaagtggata
     1501 taatagtgat cgaatcctat tgctgatttc acctaaattt attaaaatcg atatggatat
     1561 aattcgtaat attgatagtg atcctgataa gtgtaaaatt gttgaaaaca ttgttaatta
     1621 tgctcatgaa cgggatatga aattaattgc tgaaggaatt gaaacgattg atgaattaaa
     1681 gcaggtgatt aagcttaaag ttgattatct tcaaggtttt ttattagcca agccgcaata
     1741 tttaccacct cggattcaag aagatgtgat taagttaatt caacttttaa atgaaaaata
     1801 gatatagaag gtacagtaat gtatctaaat atatcttttt gtttagataa atagatttgt
     1861 tttaaatggg agcctgtcca agaaaaactt gaacaggctt attaatgcta taaagctttt
     1921 ttactattta gattcttaag ataataacta acactccttg tcaggatgat taaaacagga
     1981 atgctaataa caatcgtaat taggataatt tgaattaact taggtgttaa tacatgcatt
     2041 tctaaaagtg gccaaaaaat cggtgtagag aggataaagc caataatatc aattattaaa
     2101 actccacctc gataccagtt taatggttta taaatacgat aaatcatgtg aatagacaca
     2161 aaagcagttg taaaatatag gactgaaaaa gtttctgcta acgacaattg taaggttggt
     2221 gcgagtaatc ttataccagc aacgcttaaa actattgcta atgcgttagg taaggaaaaa
     2281 cgcatagcat gatgaccaat tgattctttt ggtttttcga tatttcgttc aaataaaatc
     2341 ataaatgaag ggaaaccttc aataaacata tccaataaag tcatttggaa aggaataaaa
     2401 ggataaggta tattcattaa aatagataat attgaaagta aaatcgtgta aattgttttc
     2461 aagtaataca ttgacgcaga acgtgtaaca ttattaataa ctaggcgacc ttctcgaaca
     2521 acttctacta atgtttgtaa tttaccatca ataataataa attgcgatac ttggcgggca
     2581 atatcggaac cagaacccat cgcaattgaa acatcagcat cttttaatgc taaaacatca
     2641 tttactccat cacccgtcat cgcaaccttt tgattatgtt tttgtaaaca taagatcatc
     2701 tggtgctttt gaaaaggact agcacgacca ataacattat agtttaaaat tgcatcttct
     2761 aattgttgat cagtaacgat tgttgaagca tcaatgtatt tttctgcatt agcaacacct
     2821 gcttgactag cgattgcact aactgtcgct ggattatcac cagatataac ttttacagca
     2881 acatcattat cactgaaaaa tttaagggct tcatgagcat ctttccgaat aggatcttta
     2941 ataacaatta gcccaatcgg tgttgtttgt cctaaagttt cctgtaagat atttaatgtt
     3001 gggtgatggc caattaataa aacacgtgcg ccgctttttt gcatctcaat aattcttggt
     3061 gataattgat aatcaggacg aattatttct ggtgctccaa caataattgt tcctattgaa
     3121 tctagttcaa tggcgctcca tttccgggct gaagaaaaag ctatacggtc taatgtttca
     3181 tactttgcag ggccagtaaa atagttacta agtgttttaa aagtaatatt attatcaaga
     3241 gatcctttaa caaaggaaca cataatctca tccagatcaa gatttaatgg attttttaat
     3301 ttaatgactt gttcgatttc catttttcct tcagtcaagg taccggtttt atctaaacat
     3361 agaacatcaa ttcgtgataa ggtttcaata ctaaacattt cttgaactaa agtgttcatt
     3421 ttccctaatt taacaacact agcagctaaa gatacacttg ttaatagaac tagtccttta
     3481 ggaagcatcc ctagcagagc tgtagcagtg ttgacgattg tgcttgtaat tccttgatca
     3541 cgaatgatat aagcctgata gagcattaaa atacttaatg gtaaaactaa ataactagta
     3601 taacgagtaa ctttattaaa agtgtttaat aagacagaac taacaggctt acgagttctt
     3661 gcctgatcag taattttatt tgcatagtta tcttttccaa catgttcaac cttaccatag
     3721 caagcaccgc tcaaaataaa actaccagat aataaatggt caccaggccc tttagaaatt
     3781 ggatcagctt caccagttaa aagtgattca tcaacttcaa cattattttc aataataatt
     3841 gaatcagcac tgatttgatt accggtttca aaataaacta catcatctag aacgatttct
     3901 tcgttatcga ttgaaatcgt ttttccctca cgaataactt tagtttttgg cgaaacgatc
     3961 aagtttaatt tagcaaccat atttttagat tttatttctt gtcgcgctct aataacagta
     4021 tttgaaatga caataacaac aaaaaataaa cttgaatatg cttgaacagc aattagagca
     4081 gcagcaataa taaaattata ggcattaaag aggttaaaaa catgccccat aataatttga
     4141 aaattacttt taactactgg tttaggggaa aggttcactt tcccttcact aatacgcttt
     4201 gcaacttctt gtttatttaa acctttaatt tttcccataa ataacatcct cataaaaaac
     4261 aaaatttatc taattgtaat ataactacaa ttgctttgca atgaattagc aagaattaac
     4321 caatacataa caattataat cataacacgc tattttaaaa attctatcat aataaatcct
     4381 aagaatctct taataaatct taaaaataaa aatagtttta ttgaaatagg ttccatcttt
     4441 tgaaaaatca aattctagag ccaatttcga aaactacttt taattattaa tatgatgatg
     4501 aggaacataa gaattgtcat ctatactttt aaaagaataa cctagtgcct gataatgctc
     4561 gattattgcc ggaagggcat ctaccgtggt atttttggca tctgtatcat gagcaagaat
     4621 cacttgatta ttggcatttc cagctgtagc agtagcaatg atttgattaa ctggaatatt
     4681 gtttccaccg gcatcacctg taccataatt ccaatcataa aattgataac cacggtcaat
     4741 tacttcgttt gctagaacac tcatgattcc aggacaatat ttacgtgaaa ttgtatttga
     4801 agctccacca gggaaacgaa tatactttgg aataaagcca atttcatttt taaccatgtt
     4861 tccaactctt tctaaatcat taaaatatgc ggttactgaa gtataaacag ttttataatc
     4921 agggctgtaa gtatgtaaac cgattgtatg acctcgttga tatgcttctt tgatcaagta
     4981 attatagttt tggtttgtac cagtcacaaa gaaagttgct tttgcatgat atttgtcaag
     5041 aatatctaaa atacgtttgg tattttttga aggaccatca tcaaatgtca aataaataac
     5101 tttttcttca gtttgagcat aaactccaat ttctttcttt tcaacaactg aaacttttct
     5161 tttagcaact attttattgc cagacctatc tttgcaagta taggttacaa tataatcacc
     5221 taatttatcc aatcttactt ttttgttatc aatagtcaat tgtggatgtg gatcacgatt
     5281 atcatttcct ttaacgccag ctaaataatc aaccttacca tttaaagtga cagttagatc
     5341 attaagacca gaaatagttg gttttgttgt atctttagat aaaactgtaa cagtagcgtg
     5401 ctttgttgat ttattattac tttcatcgct aacaattact gtcactttga cttttccttt
     5461 ttttgaaaaa ttatatgcat ctttaaaaga aatagttgtt tttgtttcat caacaatgtt
     5521 atcaactaac attgttggat caacatccat tcctgcatct atttcaatat tattaatttc
     5581 aaatttaggc ttagttttat caactacgtt aatatttact gtgaactttt tgccctgata
     5641 aatataggta acaggatagc tgccaatttt atcatttttt aatttagatg tgtcgacctt
     5701 aatatcggat gtttggacat ttttaatatt tttaattatt ttttgataat caaactcatg
     5761 gttgatttct agtattgggt ctttgacaaa agttaacaat tggttatttt tttgccagat
     5821 taaataaact gtactaataa ttaaaataaa taatactgaa ataaaggtaa ttttttgttt
     5881 ttttgaaaat ttattgaata acttcatttt tctcgacctt tccataaaca ttnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggttt
     6001 caaaaattta ttgtcaataa atgtcatgaa gttgtataat tttttttaaa tatggtataa
     6061 tgccaaaaaa ggaaagaggg aacaaaaatg tccaatatat atgattattt aaaatggcga
     6121 ggtgatctgt caatgagaat agatccattt aatgatattg atggcttgat tttaagtgaa
     6181 ttagtttatg ttgattttga taaaatagtt cccacatttc ccaatgaaca aagttattgt
     6241 ttaaaagatg ttgctaataa ttttagtcaa gtaaataata ttgaagaatt actagatgag
     6301 atatctttca ctaaggaatc aattacatta ttacaagaat tagctaaatg tccgcgttat
     6361 caaaatattc tgctatctaa ttatattaat gaactggact atcaaagtat aaaacaattt
     6421 gcagcaatta cttttcaatt agaagatagt agtatttatg ttgcttttcg tggcactgat
     6481 gatacaattt taggctggcg tgaggatttt caaatgactt atcaatttcc agttgcttct
     6541 cagcaaagag cagttgagta tttggctaaa attgctgaaa tcgatttttc taaatcactt
     6601 ttttatcttt tatgccattg tgattataaa atgaagtggt atcaaatttt taggatatat
     6661 atcacccaaa agatatctgg cgttaaaatt cgtgttggtg gtcattcaaa aggaggaaat
     6721 ttagcagttt atgcatctag ttgtgtgaat tctaaaatat ctaaacgaat tattgagatt
     6781 tataataatg acggccctgg ttttaatcaa aaaatgctaa atacagataa ttaccgcaat
     6841 atttctacta ggataaaaaa attcattcca gaaagttccg tattcgggat aatgcttgag
     6901 aatcttgaag aaaaaatagt agttaaaagt agcgctaaag gtttaatgca acatagtggc
     6961 tttagctggt cagtagaagg aacacaattt gtttgtgcta ctgaaatgag taaggatagc
     7021 caaactatgg atgcagcttt taaatcatgg ctaagtaaag tcgacagtaa aacacgaaag
     7081 gatgcagtta ctgcagtatt ttcaatttta gaagcagcta aaattgaaaa aattaatgat
     7141 ttttcagaaa aatcattatc acatctaatt acggctatta aagaaatcaa aaatatgaac
     7201 agcgatacaa gaaatgcatt aattcaattt tttaaaacgt taattgtaga gacatacgat
     7261 tgtagtcgta aagaacataa gaaaaaagat actaatgaaa ataattcata aagatttaat
     7321 aatttcaaat tatacttaat gtataatttg aaaagagggt taacatgaat aagtttcaaa
     7381 atgcgctgta tcgctttatg tcaggtcgtt atggatcgga tcaactaaac aattttttat
     7441 taatatttgc attaatttta ttaattttaa atctttttgt aattcgcaat ccatatcttg
     7501 caacaattat ttggataatt ttaattatta atatttttag gacctattct cgtaatattt
     7561 ataaaag
//
LOCUS       DAOMTA010000032         7538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_123_length_7538_cov_2.213150, whole genome shotgun sequence.
ACCESSION   DAOMTA010000032 DAOMTA010000000
VERSION     DAOMTA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_7538_cov_2.213150"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>285
                     /locus_tag="PLK68_01655"
     CDS             <1..>285
                     /locus_tag="PLK68_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89145.1"
                     /translation="DFDQTKVLITNIIKNTADDYDLFLSENSINNLSVHLTIAIIRIK
                     SNNYIPMSNSQIASYKQDHSYPYAKMLCDRLAREFEIDFPEAEISLVSMYL"
     assembly_gap    286..295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <296..743
                     /locus_tag="PLK68_01660"
     CDS             <296..743
                     /locus_tag="PLK68_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89146.1"
                     /translation="LDYRKDSEIDDMKNHRHSFFSFRPFLSKNQKLDLEINSGFDLLD
                     DQVFKILRETMYTIYQDYHKDFRNDDKLFVAIGLHLEPALERLANGQLVENPLKEKII
                     ERHQEEFNYSKILNDVVRHDLNLSFDDDELAFIALHFVVANNRIDE"
     gene            complement(861..1511)
                     /gene="deoC"
                     /locus_tag="PLK68_01665"
     CDS             complement(861..1511)
                     /gene="deoC"
                     /locus_tag="PLK68_01665"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM89147.1"
                     /translation="MNYNKMIDHTVLKADATKAMVAKIIDEAKEYNFASVCVNPTWVA
                     YCAQALVDSDVKVCTVIGFPLGANTSTVKAFETKDAIANGADEIDMVINIGALKDGNT
                     DLVFNDIKAVVDAAAGKCVKVIIETCLLTDEEIVTVCKLAKEAQATFVKTSTGFSTRG
                     ATPEAVSLMKQTVGDDLEVKASGGVRTIEDMEKVVAAGATRIGTSAGCKLVKNNNS"
     gene            complement(1501..2967)
                     /locus_tag="PLK68_01670"
     CDS             complement(1501..2967)
                     /locus_tag="PLK68_01670"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRM89148.1"
                     /translation="MKRLNELFDTDNDMKIYSIHSDSRYVKPYSIFFCIEGLSVDGHR
                     YVEDAVFQGAKVIVHSKNLNYYHDKIIYFRVENTLVELNRVSNLFYDCPSNKMKIIGV
                     TGSSGKTVVASMIKDAMAKYCSSGYIGTISLEYNGRKEECPYTTPEALYLQRKLFEMN
                     RAGVKVVAMEASSHGLALGRVDGINFNIAVMTNIGAEHLDFHGTKEQYVLAKQKLFEM
                     IKPTGWAILNSDDLNFMTLKNNTQGRILTYGIEIESDIMAKNIQLHLDHSEFDISFKG
                     NTFHVNSPVIAMFNIYNVLALTGVLIAMGCDDKMVIDAVENVKPIEGRMELIQTEQQF
                     AVIIDYCQHISNYEAIFEYVDGVRQGHGRIIAVLGAPGKRNYKLRKELGQLANRYLDH
                     VILTQLDDRGEDVYEICKTIQKEIVDINSVIIESRQIAIEQAIEIACKDDIILILGKG
                     HEKFISLDVGQVDYPGDSQIVKEALERVYYKGEDEDEL"
     gene            complement(2967..4253)
                     /gene="mtaB"
                     /locus_tag="PLK68_01675"
     CDS             complement(2967..4253)
                     /gene="mtaB"
                     /locus_tag="PLK68_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRM89149.1"
                     /translation="MKTVAFHTLGCKVNTYESNAMLKIFNEAEYQEVDFKQVADVYVI
                     NTCTVTNTGDSKSRQMIRKAIRKNPKATICVVGCYSQTAPEEIEKIEGVGVVLGTQYR
                     SDIVKYVDEHLETGEMVIKVDNVMNLRKFEDLNIDRFKNTRAFLKIQDGCNNFCTYCI
                     IPYARGRVRSRQKESVLNQAQRLVDNGYVEIVLTGIHTAGYGEDLDDYSFYELLVDLV
                     KIKGLKRLRISSIETSQISDEIIDLIGSNEIIVDHLHVPLQAGSDATLKRMNRKYTTA
                     EYLEKINKIRSYLPNIAFTTDVIVGFPGETDEEFEETYNFIKQVNYSELHVFPYSPRK
                     NTPAAKMKDQVNDQIKHERANRLLQLSKELNHEFALKQIGKTLKVLFEKRDGEYLIGH
                     AGDYLKVKVKTADNLIGEIVTIKIDKYDEILEGRVV"
     gene            complement(4250..5035)
                     /locus_tag="PLK68_01680"
     CDS             complement(4250..5035)
                     /locus_tag="PLK68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89150.1"
                     /translation="MSILDLLNKYRYEIKLNKTGLYNFVTGGNKELAGEGFNYKLKTP
                     EDLFAYEVILNGIRTKIAIDECYNKFMAANYDIFEYVNYEEKRKMFNHDEEKIINNFP
                     SFQDHQSGEIIYIPYLEPFINRYYANDYQLVTLKQHQVYLNSYAKTIDKVIELYGIQP
                     YNSQFSSLQLVGQDDESYYFYHDDFKTVYQFNGQNYRIANEINLIDRYTKEYPNLNLI
                     KEAMVKLANSQDDEEILEFLYENKFVGDKTYKKLIKKLKKVSK"
     gene            complement(5035..5772)
                     /locus_tag="PLK68_01685"
     CDS             complement(5035..5772)
                     /locus_tag="PLK68_01685"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRM89151.1"
                     /translation="MQRYFINEDLTSKQQLILPDDDLHHVKKVMRNINGDEIICIDNA
                     GQVYYGVIEDIEIGLVKIDKRLEENNELDVEITLVYALPKGDKFELVLQKATELGVKR
                     IVPIQTKRCVVKMDEQKFAKKITRYRKILKEAAEQSWRNFIPEITNVIKLEQLDQYLG
                     DHNLVAFEELAKQGEHMVLKQTLDQLSSGDKITIVVGSEGGFELDEIEMMNKLGIKAC
                     SLGKRILRSETAPLYFLSVIGYAREIG"
     gene            complement(6407..7222)
                     /locus_tag="PLK68_01690"
     CDS             complement(6407..7222)
                     /locus_tag="PLK68_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRM89152.1"
                     /translation="MERFQRVVMKFISPQGNLFRYSLSFCVLLALLPALIIFLKVFQT
                     DILSAPNLINLLYDYVPETILAPFVEYVMSQEYTTFVSLIISMGFSIYLASNAFYSFM
                     LISMTDEGFDTYAILVRIKAIVVFVLLVIGLVGLTLFNYLVPINSTIVMLAGLFIVFY
                     CFYRYLSFEKKPLSYGLIGAAFTSACIVLIGVFFFYIVNHYTRYNALYGPLASLVIML
                     ISIYLISSVIYFGYCLNHEYGKSFSKRTYKHQKFYDYGNNFLDKIVEKFGKLI"
     gene            complement(7308..>7538)
                     /locus_tag="PLK68_01695"
     CDS             complement(7308..>7538)
                     /locus_tag="PLK68_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89153.1"
                     /translation="LSDRLSDKKITITVSDRAKAVISEQGYDVTFGARPLKRFIQSRI
                     ETLVAREMIKGDIKNGSHVLVDFDDDFKLAIQ"
BASE COUNT         2564 a         1431 c          845 g         2688 t
ORIGIN      
        1 gattttgatc aaacaaaagt actaattact aatatcatta aaaatactgc tgatgactat
       61 gatttatttc tttcagaaaa ttcaatcaat aatctctcag tacatttaac cattgcaatt
      121 atccggatca aatcaaataa ttatattccc atgagtaaca gtcaaatcgc ttcatataaa
      181 caagatcata gttatccata tgcaaaaatg ttatgcgacc gtttagcacg tgaatttgaa
      241 attgattttc ctgaagctga gatttcattg gtttccatgt atctannnnn nnnnncctgg
      301 attatcgtaa agattctgaa atcgacgata tgaaaaatca tcgtcattct tttttttctt
      361 ttcgaccttt tctatcaaaa aatcaaaaat tagatttaga aataaattct ggttttgatt
      421 tacttgacga tcaagttttt aagattttaa gagaaacaat gtatacaatt tatcaagact
      481 atcataaaga ctttagaaat gatgataaat tattcgttgc tatcggttta catttagaac
      541 cagctttaga acgtcttgca aatggtcaac ttgttgaaaa tcctttaaaa gaaaaaataa
      601 tcgaacgtca tcaagaggaa tttaattata gtaagatttt aaatgatgtt gttagacatg
      661 atttaaatct atcattcgat gatgatgaat tagcttttat cgcattacat tttgttgttg
      721 ctaataatcg catcgatgaa taaaataaat atctaaataa aaatatcttc atttcgttgt
      781 gcttagtcag ttttctaact aaacagtaat aaatgaagat attttttatt tatttaaaat
      841 aaccaactca cttattcgtg ttatgaatta ttatttttaa ccaatttaca tcccgcacta
      901 gttccaattc ttgtagctcc tgcagctaca actttttcca tgtcttcaat tgtccgcact
      961 ccaccagatg cctttacttc taaatcatct ccaacagttt gtttcatcaa agaaacagct
     1021 tcaggtgtag cccctcttgt tgaaaaacca gtcgatgttt taacaaatgt tgcttgagct
     1081 tccttagcta atttacatac tgtaacaatt tcttcatcgg taagaagaca ggtttcaata
     1141 atcactttta cacatttacc cgcagcagca tcaacaactg ctttaatgtc attaaagact
     1201 agatcggtat tcccatcttt tagtgctcca atgttaataa ccatatcaat ttcatcagca
     1261 ccattagcaa tagcatcctt tgtttcaaac gctttaacgg tacttgtatt agctcctaaa
     1321 ggaaatccaa tcacagtaca tactttaaca tcactatcaa ctagagcttg agcgcaataa
     1381 gctacccagg ttggattaac acatactgaa gcaaagttat actcttttgc ttcatcaata
     1441 attttagcaa ccattgcttt agttgcatca gcttttaata cagtgtggtc aatcatttta
     1501 ttataattca tcttcatctt ctcctttata atatactctt tcaagtgcct ctttaacaat
     1561 ttggctatca ccaggataat ctacttgtcc tacatcgagt gaaatgaact tttcatgtcc
     1621 cttaccaaga atcaaaatga tgtcatcttt acaagctatc tcgattgcct gttcaattgc
     1681 aatttgacgt gactcgataa ttacactatt aatatcgaca atttcttttt gaatcgtttt
     1741 acaaatttca taaacatctt caccacgatc atccaattgt gtcaaaataa catgatctaa
     1801 gtatcgattc gctaattgcc caagttcctt acgtaatttg taattacgct tcccaggagc
     1861 ccctaaaacc gcaataattc gcccatgccc ttgtcgaact ccatcaacat attcaaaaat
     1921 agcttcataa ttactaatgt gttgacaata atcaataata actgcaaact gttgttctgt
     1981 ttgaatcagt tccattcgcc cttcgatcgg ttttacattt tcaactgcat caatgaccat
     2041 cttatcatca catcccatag caatcaacac accggttaaa gccaagacat tataaatatt
     2101 gaacattgcg attactgggg aattaacatg aaaagtattt cctttaaatg aaatatcaaa
     2161 ctcactatga tctaaatgta attgaatatt tttagccata atatcacttt caatttcaat
     2221 tccgtaagtc aaaatgcgtc cttgggtatt attttttaaa gtcataaaat ttaaatcatc
     2281 actatttaaa atcgcccatc ccgtcggttt aatcatttca aataattttt gtttagctaa
     2341 aacatattgt tcctttgttc catggaaatc taaatgttca gccccaatat tagtcattac
     2401 agcaatatta aaattaatcc cgtcaacacg tcctaaagcc aaaccatgac ttgatgcctc
     2461 cattgcaaca actttgaccc cagctctatt catttcaaac aattttcgtt gcaaatacag
     2521 agcttctgga gtggtataag gacattcctc tttacggcca ttatattcta atgaaatcgt
     2581 tcctatatat ccactactac aatattttgc catagcatct ttaatcattg aagcaactac
     2641 cgtcttacca ctgctgccag taactccgat aattttcatt ttattacttg gacaatcata
     2701 aaaaagattt gaaacacgat taagttctac taaagtattc tcaacccgaa aataaataat
     2761 cttatcatgg taataattaa gatttttgga atgaacgatt acttttgcac cctgaaaaac
     2821 agcatcttca acataacgat gtccatcaac acttaaacct tcaatgcaaa agaaaatcga
     2881 ataaggtttt acatatcttg aatcactatg aattgaatag attttcatat cgttgtcagt
     2941 atcaaacaat tcatttaagc gtttcattaa acaacccttc cttccaaaat ttcatcatat
     3001 ttatcaattt tgatcgttac gatctcacca attaaattat cagctgtttt aactttaact
     3061 tttaaataat caccagcatg accaattaaa tattctccat ctcttttttc aaataaaact
     3121 tttaaagttt ttccaatctg ttttaaagca aactcatgat ttagctcttt cgataactgc
     3181 aataaacgat tagctcgttc atgcttaatt tgatcattta cctgatcttt cattttagct
     3241 gctggcgtat tttttcgtgg tgaatatgga aaaacatgta attcactata attaacttgt
     3301 ttaataaagt tatatgtttc ttcaaactcc tcgtccgttt ctccaggaaa accaactatt
     3361 acatctgtcg taaaagcaat atttggtaaa taactgcgaa tcttattaat tttttctaaa
     3421 tattcagctg ttgtatattt acgattcatc cgcttcaaag tagcatcact tccagcttgt
     3481 aaaggaacat gtaaatgatc tacgataatc tcatttgagc cgattaaatc aataatttca
     3541 tcactaattt gacttgtttc aatcgatgag attcttaatc gctttaagcc tttgatcttc
     3601 actaaatcta ctagcagttc ataaaaacta tagtcatcta gatcctcacc ataaccagct
     3661 gtatgaattc cagttaagac aatttcaaca tatccattgt caactagcct ttgggcttgg
     3721 tttaacacac tttctttttg tcttgaacgc actcttcctc tagcataagg aataatacaa
     3781 taagtacaaa aattattaca tccatcttga atttttaaaa atgcccgtgt atttttaaat
     3841 cgatcaatat ttagatcttc aaacttcctt agattcatta cattatcaac tttaatgacc
     3901 atttctccag tttccaaatg ctcatcaaca tatttgacta tatcactacg gtattgagtt
     3961 ccaagaacaa caccaacacc ttcgattttt tcaatttcct ccggggctgt ctgactataa
     4021 cacccaacta cacaaatagt cgctttaggg ttcttacgaa ttgccttgcg aatcatttga
     4081 cgtgatttac tatcacccgt atttgtaact gtacaagtat tgataacata aacatcagca
     4141 acttgtttaa agtcaacttc ttgatattct gcttcattaa aaatcttcaa cattgcgttg
     4201 gattcatatg tatttacttt acatcctaat gtatgaaatg ctactgtctt catttactca
     4261 ccttttttaa tttctttatt aattttttgt aggtcttatc accaacaaat ttattctcat
     4321 ataaaaactc taatatttct tcatcatcct gactattagc taatttaacc attgcttcct
     4381 taatcaaatt aagattaggg tattcttttg tatatcgatc aattaaatta atttcatttg
     4441 caatacgata attttgtcca ttaaattgat ataccgtttt aaaatcatca tgataaaaat
     4501 aataactttc atcatcttga ccaaccagct gcaacgatga aaattgtgaa ttgtatggtt
     4561 gtattccata taattcaata actttatcta ttgttttagc ataactattt aaataaactt
     4621 gatgttgttt tagtgttact aattgataat cattagcata atatctgttt ataaacggtt
     4681 ctaaataagg aatataaatt atttctccac tttgatgatc ttgaaaactt gggaaattat
     4741 taattatctt ttcttcatca tgattaaaca tttttctttt ttcttcataa ttcacatatt
     4801 caaaaatatc ataattagct gccataaatt tattataaca ctcatcaatc gctatttttg
     4861 tcctaattcc atttaaaata acttcatatg caaataaatc ttccggtgtc tttagtttat
     4921 aattaaagcc ttctcctgcc agctctttat taccaccagt cacaaaatta tataatcctg
     4981 tcttatttaa ttttatttca taacgatatt tatttaataa atccaatata ctcattatcc
     5041 aatctcccta gcataaccaa ttacacttaa aaaatataat ggagctgttt cacttctcaa
     5101 aattcttttt cctagtgaac aagctttaat tcctaattta ttcatcatct caatttcatc
     5161 aagttcaaag ccgccttcac ttccaactac aatagtaatt ttatcacctg aagataattg
     5221 gtctaatgtt tgttttagca ccatatgttc accttgttta gcaagttctt caaaagctac
     5281 tagattatga tcacctaaat attgatcaag ctgttctagt ttgattacat tagtaatttc
     5341 tggaataaaa ttacgccatg actgttctgc agcctctttt aatatttttc gataacgagt
     5401 gattttttta gcaaactttt gctcatccat ctttactaca caacgctttg tttgaattgg
     5461 aacaatacgc ttgacaccaa gttctgtagc tttttgtaat actaattcaa atttatcccc
     5521 tttgggtaga gcataaacta aagttatctc gacatctaat tcattatttt cttcaagccg
     5581 cttatcaatt ttaactaaac ctatctcaat atcttcaatt acgccataat atacttgtcc
     5641 agcattatct atacagataa tttcatcacc atttatattt cgcattacct ttttaacatg
     5701 atgcaaatca tcatccggta aaattagttg ttgtttactt gttaaatctt catttataaa
     5761 atatcgttgc ataatagacc tccgctaaca ttctaacacg aattatcgac agagaaaagc
     5821 aataaacgac atcacttttt ttactttttc ttctattttt atcagtatta atttagtaaa
     5881 ttaaaaagtt gatttccata agcaaaatgt agtgaactga ataagttgtg gtccaactta
     5941 ttcagttcac tacaaatatc acctttttaa attattattg tcccctatta caatttcaca
     6001 acttgttaaa actcgatagt cactcgctac ctattcacgt aactctggaa ttatttgtgg
     6061 tttggcattt ttaccagtta tttctgtttc tttagcagaa atttttatca ttgcattctt
     6121 ttccgtcata aatgatgacc tatctgtttc attcgttact ttagataata atttaactgt
     6181 ctgagtgtga aattaccctt tgccatctaa tgatatcata gctctcttat cactccctcc
     6241 caataaaaaa accaaatata attttgccta aaaacacaaa aatatatctg gtcttacctc
     6301 ctcaaagcaa caatccttct atggtataaa tataacacgg aaatgaatat tatcaatatt
     6361 tttagtgaaa ccattaataa aaatataatc caatttcagt aaaatttcaa attaattttc
     6421 caaatttttc tacgatttta tccaaaaaat tatttccata atcataaaac ttttgatgtt
     6481 tgtaagttct tttagaaaaa gacttaccat actcatgatt taaacaataa ccaaaatata
     6541 ttacactaga aattaaataa attgaaatca gcatgattac taagctggca agcggcccat
     6601 acaaagcatt atatcgtgta taatgattga caatatagaa aaagaatact ccaattaaaa
     6661 caatgcatgc actagtaaaa gcagctccaa tcaagccata tgataatggc tttttttcaa
     6721 atgataaata tcgataaaaa cagtaaaata caataaataa accagccaac ataacaattg
     6781 tgctgttaat tggtactaaa taattaaata atgttaatcc tactaaacca attactaaca
     6841 aaacaaaaac tacgatagct ttaattcgca ctaaaatcgc ataagtatca aaaccttcat
     6901 cagtcattga aatcaacata aaagaataaa aagcattact cgctaaataa atagaaaatc
     6961 ccattgaaat aatcaatgat acaaatgttg tatattcttg tgacattaca tactcaacaa
     7021 aaggtgctaa aattgtttca ggaacataat catatagtaa attaattaaa ttaggcgcac
     7081 ttaaaatatc cgtttgaaaa acctttaaaa aaataatcag tgccggcaat aaagctaaca
     7141 aaacacaaaa acttaacgaa taacgaaaca agttcccctg gggcgaaata aatttcatta
     7201 caactctttg aaatctttcc ataatccacc atccctcttt attgttaact atatcacaaa
     7261 aatgcataca aaaaaatagt taaaggtgtt caaagaacac ctttaactta ttgaatcgct
     7321 aatttaaaat catcatcaaa gtcaactaat acatgacttc catttttaat atcaccttta
     7381 atcatttcac gagcaaccaa tgtttcgatt cgtgactgaa taaaacgttt tagtggtcgt
     7441 gcaccaaatg ttacatcata accttgttca ctaattacag ctttagctct atcacttact
     7501 gtaatagtga tctttttatc acttaaacga tcagatag
//
LOCUS       DAOMTA010000033         7474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_124_length_7474_cov_2.638631, whole genome shotgun sequence.
ACCESSION   DAOMTA010000033 DAOMTA010000000
VERSION     DAOMTA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_7474_cov_2.638631"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PLK68_01700"
     CDS             <1..1203
                     /locus_tag="PLK68_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89154.1"
                     /translation="EVYQSTNEGLRALVKEGQAELAKFDAGNKNGNIAKDDYEVFEQK
                     FEDYLALADGTEISTEEIGKLLKEVTAIINSIEDSVIYSKNELNELYQKVSLYQEKDF
                     TKDSFVDFNTKLTAIKGLIDNADDYIKVNEAMGQLQTLDSSLVKLDRSKLKTAIESYD
                     NFQETDYTTSSWQTMKTIVDAAKLVHENVSLNQVDVDNAIAALNEINLEKRGNTKELA
                     KLLKEYDEADYTISTWAKFAGVYQAANAMVVDNSDVNQEQVDAMVALVREAAMKLLEL
                     GNSKDLEKVINKITEAIKDLDQANYDQDSWNKLQTMIKAAKELLDSKDVSQAELDAML
                     DSLNQVYDALKPIEVKPGEETETPTNKPVTDTSDKSVKTGDDVNLFGYAGLLGIALMG
                     LICRKKFD"
     gene            1538..2149
                     /locus_tag="PLK68_01705"
     CDS             1538..2149
                     /locus_tag="PLK68_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM89155.1"
                     /translation="MEKILRKGFISLLSLLMVFTTFNQLITNVAAEQEYENIALDKVV
                     VASSAIADNEGSNAVDGKIDTKWEVGNISTNPSIIVDLGKEESFEKITLKWASNFAFR
                     YKLYVAKTDQKYGQVLFHEEGTGGDEEWEMEENTIARYVKIELIEAKSGESTIGLKEL
                     EVLRVKKDTSNDPVNIALNKPAYASGNEANLDRLGPGGAFDGK"
     gene            2139..>3650
                     /locus_tag="PLK68_01710"
     CDS             2139..>3650
                     /locus_tag="PLK68_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM89156.1"
                     /translation="MVNDYNERWSSGNITKSPQWIYVDLEAEMTFSSIHIFWENAFAT
                     KYVLQYSNDVGTDKNWITFVNVDDGKGSWEKFDFDPITARFVRLYATASNGVGSAKPI
                     SVWEMEIYEEVNNSLTVDDIAKDFTIGTITSNMDKMPVYHYDNDNIVAEIFCSDTDFV
                     VEDNGMIHKPLVDKEVLVTYIIKDKRTNATKEMKNIPVIIPGQNSVALDSNPVPTTVP
                     TIQEWNGATGTYQLTNNSRIIVQDESLYKAAQITKDDIKDLFGLELEISNTTVKKGDI
                     LLTKENADTTIGDQGYTLEIGDSITIKSTTYQGIFFGTRSVLQALVASGDALTINKGE
                     ARDYAKYEYRKFMLDVARKYMPMWYLKDFVKYASWYKFTDIQVHLNDTSYNGHSRFRL
                     ESDIPGLTATDGYYTKGEYRDFQYYSEDYGIRIVSEFDTPAHSAVFIDNNSELGFDGT
                     HLDIRLNSDKKETVYKFIADLYEEYMGGDNPVFVTDAFNVGLDEYNSAYKEDMT"
     assembly_gap    3651..3660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3661..6722
                     /locus_tag="PLK68_01715"
     CDS             <3661..6722
                     /locus_tag="PLK68_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM89157.1"
                     /translation="TQYVMDLVYNKYGKTPMAWASMGCVDSDKTKIPDYPIMDAWANY
                     AISLKSLFNQDYKLINATNKYGYIVPGGNNGYPDFAKEEEMYNNLSAGKFRDKMGAGV
                     DVAEGHPKIVGGSISLWNDRGIFNGISVYDVFARTQSILPIYAQTFWYGKDNDKSYDQ
                     FKAEVNTLGTGPNVEMDKEISSKTEKVYDFDMENTSKQGDNLVIKDNSGNGYDAAAIK
                     ATVETTDEGQALKLNGDGYLQMQHSALKWPYTLAFDLKIDESQTGDIVLFEETMPIEE
                     CNQWIDTKYQTRKIYLKEIDGKYKLMYDRDSYHYEHNIELEKGKLYQLAFTSDKKYTN
                     VYLDGVVKSTINGPILTNSGNKWYDSASINLPLQKIGQNLVGTLDNIKVYNRLLNNEE
                     IKNLYDTGIDVIHENIALNKEATASSSYTDYQTPNKAFDGIINQSASGPEQSRWASAR
                     THDQWIQVDLNKVYKVDQIKITWEDAYGVDYELKGSVNGKDWFTIKKVTGNVAKENNH
                     TGLGDIEARYIKLIGTKAANNAKYGYSIYEIEVYENPKNDLLRTISQVKDKLSEMNVG
                     NFHGQIKEEAYQTFTAYLTNLENEVVGKESISEEEMLKFKEELSKKYTNLKKQVVRVD
                     KSALETVLEVAESKLAEGYSEANSTVSSWQSFIQNKEAAEAMADRMNITQSDVDKMVS
                     ELQTALDNLTFRALESSFNELVALIDEVTKMETDYSAEMFKVMKGYLDQANALVALGA
                     GEVVEKDVQANLTNLANEKAILISYRELKVSVEVAKEILDKEAVNLRPATLKVLQDAY
                     EAGRKLIDDNSKELIVLQNAKQEINEAIEGLLEIVERKDLDKLIEQVKDLKEEDYTEE
                     SWKTFKNGYERAVTVNQDLDANEGAIQNAYDELLRAFNELKQVVNKDNLLLQIELAKQ
                     VLANKDKYDIELVKELEELLSQAVALIANDVSSEDVNKMSDALLTKIEAVKASQKEEP
                     KVEEQPGTEVSDKTKKNAKTGDETMLFEFAMISLVALLAVVRLRKKS"
     gene            6834..>7474
                     /locus_tag="PLK68_01720"
     CDS             6834..>7474
                     /locus_tag="PLK68_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HRM89158.1"
                     /translation="MKIKNFKRIYVDIVISCLIIAVVATFFAFKSQTISQEQVLNTLS
                     EISSQSVNVIDKEIQKNVAVLANLSIYISQEDAFDPVKIINKIKKVNEINNFKRIGII
                     DERGQSYTTDDNNILLNEQQMTRFNKAMNGEVSITDTLPDLIDGEEVSVYTLPITFDN
                     DQHCVLFGAYASRYYKETLSVSTFDGLGYSFIIKENGDKIVDSSNKTSIKFSN"
BASE COUNT         2858 a          867 c         1486 g         2253 t
ORIGIN      
        1 gaagtatatc aaagtactaa tgagggatta agagcattag ttaaagaagg tcaagctgag
       61 ctagcaaaat ttgatgctgg taataaaaat ggaaatattg ctaaagatga ttatgaagta
      121 tttgaacaaa aatttgagga ttatttagca cttgcagacg gtacagaaat ttctactgag
      181 gaaattggca agctattaaa agaagtgact gcgataatca actcaataga agatagtgtg
      241 atttattcaa aaaacgaatt aaatgaacta tatcaaaaag ttagtttata tcaagaaaaa
      301 gatttcacaa aggatagctt tgttgatttt aatacaaaat taactgctat taaaggatta
      361 attgataatg ctgatgatta tattaaagta aatgaagcaa tgggacaatt acaaacttta
      421 gatagtagtt tagttaaatt ggatcgaagt aaattaaaaa cagctattga aagttatgat
      481 aattttcaag aaactgatta tacaacgagt tcatggcaaa cgatgaaaac aattgttgat
      541 gctgctaagc ttgttcatga aaatgtaagt ttaaatcaag tggatgtaga taatgcgata
      601 gctgctctta atgaaattaa tttagaaaaa cgaggaaata ctaaagaatt agcgaaatta
      661 ttaaaagaat acgatgaagc tgattataca ataagtacat gggcaaaatt tgcaggagta
      721 tatcaggcag caaatgcaat ggtagtcgat aatagtgatg ttaaccaaga acaagtagat
      781 gcaatggttg cattagttcg agaggctgcg atgaaacttc tagaattagg taattcaaaa
      841 gaccttgaaa aagttatcaa taaaataact gaagcgataa aagatctaga tcaagctaat
      901 tatgatcaag attcatggaa taaacttcaa acaatgatta aggcagcaaa agaattatta
      961 gatagtaaag atgtatctca ggcggaatta gatgctatgt tagattcatt aaatcaagtt
     1021 tatgatgctt taaaaccaat agaagtaaaa cctggtgagg aaacggaaac accaactaac
     1081 aaaccagtaa ctgatacaag tgataaatct gtaaaaacag gtgatgatgt taatttattt
     1141 ggttatgcag gattattagg aatagctcta atgggattaa tttgtcgtaa aaaatttgat
     1201 taattaaaaa cagtattttt gtggtgaccc tctataaagt gatatgtgtc cagaattcta
     1261 gatatctatc actttagggg gtttttatat ggaacattgt gccatgttta tatcttttat
     1321 acgaaacatt gtaccgtaat tgtatagaca catttctttt atgtataaat agtgctatat
     1381 taaaaaggat tgttactttc agtaggcaag accgaaaaag cgagatgctt tttagtcttg
     1441 tcttttttta ctaaaaacat ctggcttcaa aaaaaggagg gacatgcaca gagggaaaat
     1501 aagtaagatt tttattaatc aaagaaaggg aagaattatg gaaaagatat taaggaaagg
     1561 ttttatttca ttattatcac tcttgatggt atttactaca tttaaccaac ttattacaaa
     1621 tgtagctgct gaacaagagt atgaaaatat cgcattggat aaagtggttg tggcgagtag
     1681 tgctattgcc gacaatgagg ggagcaatgc agttgatggt aagatagata ctaaatggga
     1741 agtaggtaat attagtacaa acccaagtat tatagttgat ttagggaaag aggaaagttt
     1801 tgaaaaaatt acattaaagt gggctagtaa ttttgcattt cgttataaac tatatgtggc
     1861 taagaccgac caaaaatatg gacaagtcct attccacgaa gagggtactg gtggcgatga
     1921 agaatgggaa atggaagaaa atacaattgc ccgctatgtg aaaattgaac tgattgaagc
     1981 taagagcgga gaaagtacga ttggtttaaa agaattagaa gttttaagag ttaagaagga
     2041 tactagtaat gatccagtta atattgcttt aaataaacca gcatatgctt ctggcaatga
     2101 agctaatttg gatcgtcttg gaccaggtgg agcctttgat ggtaaatgat tataatgaac
     2161 gctggtcaag tggaaatatt actaagagtc cccaatggat ctatgttgat ttagaagcag
     2221 aaatgacatt ttcatcaata catatttttt gggaaaatgc ttttgcgaca aaatatgtat
     2281 tgcaatattc taatgatgtt ggaactgata aaaattggat aacatttgta aatgtagatg
     2341 atggtaaggg aagttgggaa aaatttgatt ttgatccaat tacagctcgg tttgttcgtt
     2401 tatatgctac agcaagtaat ggtgttggta gtgctaaacc gatttctgtt tgggaaatgg
     2461 aaatttatga agaagtaaat aatagtctta cagttgatga tattgccaaa gattttacaa
     2521 tcggaacaat tacttcaaat atggataaga tgccagtata tcattatgat aatgataata
     2581 ttgtagcaga aattttttgt agtgatactg actttgtagt tgaagataat ggaatgattc
     2641 ataagccatt ggtagataag gaagtgttag ttacttatat tattaaagat aaaagaacta
     2701 atgcaactaa ggaaatgaaa aatattccag ttataattcc agggcaaaat tcagttgctt
     2761 tagatagtaa tcctgtccct actacagttc caacgattca agaatggaat ggagcaacag
     2821 gaacttatca attaactaat aattctagaa ttattgttca agatgaaagt ttatataaag
     2881 cagctcaaat tactaaagat gatattaagg atttatttgg attagaatta gaaattagta
     2941 atacaacagt aaaaaaagga gatattttat taactaaaga aaatgcggat accactattg
     3001 gtgatcaggg atatacatta gaaattgggg atagtattac aattaagtca acaacttatc
     3061 aaggtatttt ctttggaaca cgcagtgttt tacaagcatt agttgcaagt ggggatgcac
     3121 tgacaattaa taaaggtgaa gcacgagatt atgctaaata tgaatatcgt aaattcatgt
     3181 tggatgttgc ccgtaaatat atgccaatgt ggtatttaaa agattttgtt aaatatgcat
     3241 cttggtacaa gttcacagat attcaagtac atttaaatga tacttcatac aacggtcatt
     3301 cgcgttttag attggagtca gatattccag gattaacggc aacagatggt tattatacaa
     3361 aaggtgagta tcgtgatttc caatattatt ctgaggatta tggaattaga attgtttctg
     3421 agttcgatac tccagcacat tcagcagtgt ttattgacaa taattctgaa ttaggatttg
     3481 atggaacaca tttagatata cgcttaaatt ctgataaaaa agaaacagtg tataaattta
     3541 ttgctgatct atatgaagaa tatatggggg gagataatcc ggtttttgta accgatgcat
     3601 ttaatgtagg gcttgatgag tacaatagtg catacaagga agatatgacc nnnnnnnnnn
     3661 atactcaata cgtaatggat cttgtttata ataaatatgg taaaacgcca atggcatggg
     3721 cttctatggg atgtgtagat tctgataaaa caaaaatacc agattatcca attatggatg
     3781 cttgggcaaa ttatgcaatc agtttaaaat cactatttaa tcaggattat aaattaatta
     3841 acgcaactaa taagtatggt tatattgttc ctggcgggaa taatggttat ccagattttg
     3901 cgaaagaaga agaaatgtac aataacttaa gtgctggaaa atttagagat aaaatgggcg
     3961 ctggagttga tgttgctgaa ggacatccaa aaattgttgg gggttcgatt tcattatgga
     4021 atgaccgagg aatctttaat gggatttctg tttatgatgt ttttgcaaga acacaaagta
     4081 tcttaccaat atatgctcaa accttctggt acggtaaaga taatgacaag tcatatgacc
     4141 aatttaaagc tgaggtcaat actttaggta ctggtccaaa tgttgaaatg gataaagaaa
     4201 ttagcagtaa aacagaaaaa gtatatgact ttgatatgga aaatacttca aaacaagggg
     4261 ataatttagt cattaaagat aattctggaa atggttatga tgctgctgca atcaaagcaa
     4321 cagttgaaac aactgatgaa ggtcaggcat tgaaattaaa tggtgatggt tatctacaaa
     4381 tgcagcatag tgcattgaaa tggccgtata ctttagcttt tgatttaaaa atcgatgaat
     4441 cacaaacagg cgatattgta ttatttgaag aaacaatgcc aatagaagaa tgtaatcagt
     4501 ggattgatac aaagtatcaa acaagaaaaa tctatttaaa agaaattgat ggtaaataca
     4561 agctgatgta cgatcgtgat agttatcatt atgagcataa tatagaactt gaaaagggaa
     4621 aattatatca attagctttt acttctgata aaaaatatac taatgtttat ctagatggag
     4681 tagttaaatc aacaatcaat ggaccaattc ttaccaattc ggggaataag tggtatgatt
     4741 cagcttcaat caatttacca ctacaaaaga ttggacaaaa tttagtagga acattagata
     4801 atattaaagt ttataatcgt ttattgaata atgaagaaat taaaaatctt tatgatacag
     4861 gaatagatgt tattcatgaa aatattgctt tgaataaaga agcaacagct tcaagcagct
     4921 atactgatta tcaaacacca aataaagcat ttgatggaat cattaatcaa agtgctagtg
     4981 gtcctgagca atcacgctgg gcgtcagcac gtacgcatga tcagtggatc caggtagatt
     5041 taaataaagt ttataaagtt gatcaaatca agattacctg ggaagatgct tatggagtgg
     5101 actatgaatt aaaaggctca gttaacggta aagactggtt tacgatcaaa aaagtaactg
     5161 gtaatgtagc taaggaaaat aatcatactg gattaggtga tattgaagca cgttatatta
     5221 aattaattgg tacaaaagca gctaataatg ctaagtatgg atattctatt tatgaaattg
     5281 aagtatatga aaatcctaaa aatgatttac ttagaacaat tagtcaagtt aaagataaat
     5341 taagtgaaat gaatgtgggt aatttccacg gtcaaatcaa agaagaagct tatcaaactt
     5401 tcacggctta tttaaccaat cttgaaaatg aagtggtagg aaaagaaagt atcagtgaag
     5461 aagaaatgct taagttcaaa gaagaattgt ctaaaaaata cacaaattta aagaaacagg
     5521 ttgtaagagt tgataaaagt gctttagaaa cagtgcttga agtggcggag agtaaactgg
     5581 cagaagggta cagtgaagca aatagtacag tttcaagctg gcaaagtttc atacaaaaca
     5641 aagaagcagc tgaagcaatg gctgatagaa tgaatattac tcaaagcgat gttgataaga
     5701 tggtatctga actgcaaaca gcattagata atttaacatt tagggcattg gaatcatcat
     5761 ttaatgaact ggttgcactg attgatgaag ttactaagat ggaaactgat tattctgcag
     5821 aaatgtttaa ggttatgaaa ggatatcttg atcaagcaaa tgccttagtt gcactaggag
     5881 caggagaagt agttgaaaaa gatgttcaag ccaatctaac aaatcttgca aatgaaaaag
     5941 ccattctgat tagttaccgg gaattaaagg taagcgttga agttgcaaaa gagattttgg
     6001 ataaagaagc agtaaatctt cggccagcta ctttgaaagt attgcaagat gcttatgaag
     6061 caggaagaaa actaatagat gataacagta aggaacttat agttttacaa aatgcaaaac
     6121 aagaaatcaa tgaagcgatt gaaggacttt tagaaatagt agagcgcaag gatttagata
     6181 aattgattga acaggttaaa gatttaaaag aagaagatta tactgaagaa tcatggaaaa
     6241 cgtttaaaaa tggttatgaa agggccgtca cagttaatca ggatcttgac gctaatgaag
     6301 gtgctattca aaatgcctac gatgaattac taagagcttt caatgaatta aagcaagttg
     6361 ttaataaaga taatttacta ctccaaattg aattggcaaa acaagttctt gctaacaaag
     6421 ataaatatga tattgagtta gtcaaggaat tagaagaatt attaagtcag gcagttgcac
     6481 tgattgctaa tgatgttagc agtgaagatg ttaataaaat gagtgatgct ctattaacaa
     6541 aaatcgaagc tgttaaagct agtcaaaaag aggaaccgaa ggttgaggaa cagccaggga
     6601 ctgaggtatc agacaaaaca aagaaaaatg ctaaaacagg tgatgaaaca atgctctttg
     6661 aatttgcaat gatatcatta gttgcacttt tagctgtggt tagattaaga aagaagagtt
     6721 aattagttaa agggtcatgt tcaaaagcga atatgaccct taattataca attaatcgat
     6781 tttattaatc gataatgata taatattgta gagataaatg tggggtgtat gagatgaaaa
     6841 ttaaaaactt taaaagaatc tatgtcgaca tcgtgatatc atgtttgatc attgctgttg
     6901 tcgcaacttt ttttgctttt aaatctcaaa caatttcaca agaacaggtt ctaaatacgt
     6961 tatcagaaat ttctagtcaa agtgtaaatg tgattgataa agagattcaa aaaaatgttg
     7021 cagttttagc aaatttatca atttatatta gccaagagga tgcttttgat cccgtcaaaa
     7081 ttattaacaa aataaaaaaa gtgaatgaaa ttaataattt taaacgaatt ggaataattg
     7141 atgagagagg acagtcctat acgacagatg ataataatat tttattaaat gaacagcaaa
     7201 tgacccgttt taataaagcg atgaatggtg aagtatcaat tactgataca ttgccggatt
     7261 tgattgatgg tgaagaagtt agtgtttata cgttacccat aacctttgat aatgatcaac
     7321 attgtgtact ttttggagct tatgcatcac ggtattataa agagacttta tcggtttcta
     7381 catttgatgg tttaggatat agttttatta ttaaggaaaa tggtgataaa attgtagatt
     7441 cttctaacaa gacaagcatt aaattttcta actt
//
LOCUS       DAOMTA010000034         7470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_125_length_7470_cov_2.562374, whole genome shotgun sequence.
ACCESSION   DAOMTA010000034 DAOMTA010000000
VERSION     DAOMTA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_7470_cov_2.562374"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLK68_01725"
     CDS             complement(<1..392)
                     /locus_tag="PLK68_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HRM89159.1"
                     /translation="MDNEDLVREYSNNLTTYEQFTNLMETYICNLLNREQISFHSITS
                     RTKSIESLSKKIELKNKYQKLDEITDLSGIRVITYYTDTVDQISKLIENEFIIDRDNS
                     IDKRKSLDPKRFGYRSLHYVVQIDPKYV"
     gene            complement(578..901)
                     /locus_tag="PLK68_01730"
     CDS             complement(578..901)
                     /locus_tag="PLK68_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89160.1"
                     /translation="MKEFPKCPVETTLLLISDKWKVLIIRDLLPGKKRFGELKKSIGT
                     ISQKVLTSNLRAMEESGLISRKVYPEVPPKVEYSLTELGYSLKPILDAMYSWGEDFKK
                     NHQSI"
     gene            1063..1914
                     /locus_tag="PLK68_01735"
     CDS             1063..1914
                     /locus_tag="PLK68_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 silent information regulator family
                     NAD-dependent deacetylase"
                     /protein_id="HRM89161.1"
                     /translation="MFQRESLDILKLSQAIKKCDAILIGAGAGLSSAAGLSYSGNRFK
                     KYFNDFITKYHLQDMYSAGFYPYPSLEKYWAYWSRHIYYNRYLEAPKDTYKKLLNLLI
                     NKDYFVITTNVDHQFQIAGFIKEKLFYTQGDYGLWQCSKPCHQKTYDNYETVVTMIKQ
                     QHDLKIPSSLIPYCPICNAPMTMNLRCDKTFVQDSGWYHAQERYYHFLNKHHYSKIVY
                     LELGVGYNTPGIIKYPFWQLTIENPKAIYACINQDIIDLPSELKKQTIMINDNIHNVL
                     SSLEKLL"
     gene            1911..2678
                     /locus_tag="PLK68_01740"
     CDS             1911..2678
                     /locus_tag="PLK68_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HRM89162.1"
                     /translation="MNQITRLQFLINYLIQENKLEIELPTEQAQLFSLYRSLVNIREA
                     KTATDKFIMIQNKMLKNEIKRKGIIDSSNFKKSMNIWRGDITQLKVDAIVNAANNQME
                     GCFIPGHNCIDNAIHTFAGVQLRNECHQIMSKQRYLEPTGKAKITNAYNLPCRYIVHT
                     VGPIVHNVLTDEKRFLLAECYRNCLKKTEAYGLKSIAFCCISTGVFNFPKKEAAQIAI
                     NTVSTFLKGSQIEKVIFNVFKEDDEMIYQQLLNNKSK"
     gene            2964..>3046
                     /locus_tag="PLK68_01745"
     CDS             2964..>3046
                     /locus_tag="PLK68_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89163.1"
                     /translation="MQAKDNKVPLGEKLSFISIFQSNLDEF"
     assembly_gap    3047..3056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3057..4926
                     /gene="ppk1"
                     /locus_tag="PLK68_01750"
     CDS             <3057..4926
                     /gene="ppk1"
                     /locus_tag="PLK68_01750"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM89164.1"
                     /translation="LYDQMLFYPSSKDNKTGMTGEEQLKACLRRITYLNKKKDRIYQS
                     IMDELKTHGWGIVKYRDLKNKEDRKYFESYFEREILPLISPQVISKRQPFPFLNNREV
                     YVVVQLESKKGKRKMGIVSCANAMDERMIAIPSMQGKFILIEDIILHFISNIFSKYTI
                     KNKGFIRVTRSADIDEDDHSLEGHEDYREMMETLIKQRRKLCPIRLEVSPGLEELEIL
                     MLMNFLNLKKHQVFECNAPLDLKFTSELRDHLRYIHPEMFYKKLEAKNSPLVENTVPM
                     IKQILKKDILLSYPYESMSPFLRLLDEASRDTNVASIKMTLYRVAKKSKIIKSLIEAA
                     ENGKEVVVLVELRARFDEENNIDWSKRLEESGCRVIYGLDGLKVHSKLCLITYKNNQG
                     VHYISQVGTGNYNENTSKLYTDLSLMTSNYEIGEEINSIFNHLCLGETENEVNLLMVA
                     PNCMITKIFEHIDNQIALAKAGKEAYIGFKCNSVTSKEMIDKLIEASQAGVKIDMIVR
                     GICCILPGVEGLTDNIRIISIVGRYLEHSRIYIFGKGSAQKVYISSADLMTRNLSRRV
                     EVAAPILDEKLRKRIITMFNTMLKDNVKACRLLEDGTYKRVKNKEPELNSQEYFFK"
     gene            4961..6028
                     /locus_tag="PLK68_01755"
     CDS             4961..6028
                     /locus_tag="PLK68_01755"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRM89165.1"
                     /translation="MRVGILTSGGDCQALNVTMRGLAKTLYRNVNDIEIIGFLQGYKG
                     LMYEDYKIMKPKDFSGIINIGGTILGTSRCPFKKMRVIEDDFDKVAAMKNTYAKLKLD
                     CLVVLGGNGSIKSANMLSQEGLNVIALPKTIDNDTWGTDYTFGYQSAIDIATTYLDQI
                     HTTAASHNRVFVIEVMGHKVGHICLSAGIASGADVILLPEIPYDIKEVAKAIKTREKN
                     GKKFSIIACAEGALSKEEATLSKKEYKAKVKARNGQSVVYEIAAELEKYIDSEIRVSC
                     IGHAQRGGQPCPYDRMISTQFGVAGARLVMGGDYGKLVILKNNGVTAIPLVESAGKLK
                     YVDAVGAKVKDAKLLGISFGD"
     gene            complement(6068..6643)
                     /locus_tag="PLK68_01760"
     CDS             complement(6068..6643)
                     /locus_tag="PLK68_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM89166.1"
                     /translation="MKTLYMMRHGQTLFNQKRLMQGWCDSPLTELGKRQARCVKEYLE
                     NNEIVFDGAYASSSERACDTLEIVTSLPYQRLKGLKEWNFGIYEGESDDLDPPFPFGD
                     FFKKFGGESENEVSERVSTTVLELMKNTDQETVLIVSHGLSCYCFAKRWEQNQQVSLP
                     NDMPNCVVLKYQFNDEQFSLVEVIDVTSELK"
BASE COUNT         2622 a         1171 c         1167 g         2500 t
ORIGIN      
        1 ttaacatact taggatcaat ttgaaccaca taatgcaacg aacgataccc aaaacgtttt
       61 ggatccagac tctttctttt atcaatagaa ttatcacgat caataataaa ctcattttca
      121 attaatttcg atatttgatc aacagtatcc gtataatatg taattaccct aatacctgat
      181 aaatctgtta tttcatctaa tttttgatac ttgtttttta actctatctt tttagataaa
      241 ctctcaatac tctttgttct actagtgatt gaatgaaacg atatttgttc acgattcaac
      301 aaattacata tatatgtttc catcaaattt gtaaattgtt catatgttgt aagattattg
      361 ctatattctc ttactaaatc ttcattatcc ataattttcc cctctaactc tctagtcata
      421 agttattatc atgcactatt atacttgtta agaaactaga aatttgacac tttatatctt
      481 tatgagtcgt atttttatca atttctattt tttactgaac cccctttaag atcattcagc
      541 catccaaaaa atattaacac tgagactcta tccattatta tattgattga tgatttttct
      601 taaaatcttc accccagcta tacatagcat ccaagattgg ttttaaacta taacctaact
      661 cagtcagcga atattcgact ttaggaggaa cttcgggata aactttacga ctaatcaatc
      721 cactctcctc cattgcccga aggtttgaag ttaatacttt ttgagaaatc gttccgattg
      781 atttttttaa ctcaccaaat ctttttttcc ctggtagcag atccctaata atcaaaacct
      841 tccatttatc acttattaat aataatgtag tttcaactgg acattttgga aattctttca
      901 tatgtgtact ccttatagtt tcttatagta actaagtata ttttataaac tatacttatg
      961 aaagtcaagt tagttacata aaagaaacta agccactttt atgtgcgtac ttcacaaata
     1021 gcaaccattg aaggataata acgatgagaa gggagtgtta taatgtttca aagagaatca
     1081 cttgatattt taaaattgag tcaagcaatc aaaaaatgtg atgcaatctt aattggagct
     1141 ggagctggac tttctagcgc cgctggtctg tcatactcag ggaaccgttt taaaaaatat
     1201 tttaatgatt tcattacaaa atatcattta caagatatgt attcggcagg cttttacccc
     1261 tatcctagtt tagagaaata ctgggcttat tggagtcgtc atatttatta taaccgctat
     1321 ttggaagccc ctaaagatac ttataaaaaa ttattaaacc tactcattaa taaagattat
     1381 ttcgtcatta caacaaatgt tgatcatcag tttcaaatag ctggttttat taaagaaaaa
     1441 ttattttata ctcaaggaga ttacggactt tggcaatgtt caaaaccatg ccatcaaaaa
     1501 acttatgata attacgagac agttgttaca atgatcaaac aacagcatga tttgaaaatt
     1561 cccagttcac taattccgta ctgtccaata tgtaatgctc caatgacaat gaatcttcgc
     1621 tgtgataaaa catttgttca agattccggc tggtatcatg cccaagaacg ttattatcac
     1681 tttttaaata aacatcatta ttccaaaatc gtttatttag aattaggagt tggctataat
     1741 acccctggaa ttatcaaata tccattttgg caattaacta tagagaatcc taaagctatt
     1801 tatgcctgca tcaatcaaga tattattgat ttaccttcgg aattaaaaaa acagactatc
     1861 atgatcaacg ataacattca taatgtctta tcgtctttgg agaaattgct atgaatcaaa
     1921 taacccgatt acaattttta attaactatt taattcaaga aaataaactt gaaattgaac
     1981 ttcctacaga acaagcacaa ctattttccc tttatcgctc attagtaaat attcgtgaag
     2041 caaaaacagc tactgacaaa ttcattatga ttcaaaacaa aatgttaaaa aatgaaatta
     2101 aacgaaaagg aatcattgac tcaagcaatt tcaaaaaatc aatgaatatc tggcgtggtg
     2161 atatcaccca actaaaagtt gatgccattg taaatgctgc caataatcaa atggaaggat
     2221 gctttatccc tggacataat tgtatcgaca atgctatcca tacctttgca ggagttcaat
     2281 taagaaatga atgtcaccaa atcatgtcta aacagcgtta tcttgagccc accggaaaag
     2341 caaaaataac taatgcttat aatcttcctt gtcgttatat cgtccataca gttggtccta
     2401 tcgttcataa tgtactaaca gatgaaaagc gctttttatt agctgaatgt tatcgtaatt
     2461 gtttaaaaaa aactgaggct tatggattaa aaagtattgc tttttgttgt atctctacag
     2521 gagtatttaa ctttcctaaa aaagaagcag ctcaaattgc aatcaataca gtatccacat
     2581 ttcttaaagg aagtcagatt gaaaaagtta tttttaacgt tttcaaagag gacgatgaaa
     2641 tgatctatca gcaattacta aataataaat ctaaatagaa tcgtaatatt tttaggtgtt
     2701 ttgacaacac ctttttcttt tgaatgattt aaatcatttt acatatttat ggttttgatg
     2761 aatactaaaa tcaaaagtgc tatactaata ttaaagaaac gttaaatttg cgtaagagtt
     2821 ttgtaagtaa tccgacttta cttgataatt tttttaataa atattatgtt atttttagaa
     2881 acaaaatagg aggcgccatt tcaatgaata aatgtaaaga aaatcgtgaa ttatcttggc
     2941 ttaaatttaa tgatcgagta ttaatgcagg ctaaagataa taaagtccct ttaggtgaaa
     3001 agttaagttt tatttctatt tttcaatcaa atcttgatga atttttnnnn nnnnnnatta
     3061 tatgatcaaa tgctattcta tcctagttct aaagacaata aaactggaat gactggtgaa
     3121 gaacagctaa aggcatgttt acgaagaatt acttatttaa ataaaaagaa agatcgtatt
     3181 tatcaaagta ttatggatga attaaaaact catggatggg gaatcgtaaa ataccgtgat
     3241 ttaaagaaca aagaagatcg taaatatttt gaaagttatt ttgaacgaga aattcttcca
     3301 ttaatctctc cccaggttat ttcaaaacgt caaccattcc cttttttaaa taaccgtgaa
     3361 gtttatgttg ttgttcagtt agaatcaaag aagggaaaaa gaaagatggg aatcgtttct
     3421 tgtgcaaatg ctatggatga acgaatgatt gcgattccta gtatgcaagg gaagtttatt
     3481 ttgattgaag atatcatcct gcattttatt tctaatatct tctctaaata tacaataaaa
     3541 aataaaggtt tcatcagagt tacaagaagt gctgatattg atgaagatga tcactcttta
     3601 gaaggtcatg aagactatcg agaaatgatg gaaacattaa ttaaacaacg tagaaaatta
     3661 tgtcctattc gtttggaagt atcaccaggc ttagaagagt tagaaatctt gatgttaatg
     3721 aacttcttga acctcaaaaa acatcaggtt ttcgagtgca atgctccatt agatttgaaa
     3781 ttcactagtg agttacgtga tcatttacgc tacattcacc cagaaatgtt ctataaaaaa
     3841 ttagaagcta aaaacagtcc tttagttgaa aatactgttc caatgatcaa acaaatttta
     3901 aaaaaagata ttttattatc atatccatat gagtcgatgt caccatttct ccgtttatta
     3961 gatgaggcta gtcgagatac aaatgttgcc agtattaaaa tgactttata tcgtgttgca
     4021 aagaaatcta agattattaa atcattgatt gaagctgctg aaaacggtaa agaagttgtt
     4081 gttttagttg aattacgagc acgttttgat gaagaaaata acattgactg gtcaaaacga
     4141 cttgaagaat ctggatgccg tgttatctat ggtttagatg gacttaaagt tcactcaaaa
     4201 ttatgtttaa taacttataa aaataatcag ggtgttcatt atattagcca ggtgggaaca
     4261 ggaaactata atgaaaatac atcaaagctt tatactgatt tatcactaat gacatctaac
     4321 tacgaaattg gggaagaaat caacagcatt tttaatcatc tatgtcttgg cgaaacagaa
     4381 aatgaagtta atcttttaat ggttgctcct aactgtatga ttacaaaaat atttgaacat
     4441 attgataatc aaattgcttt agccaaagcg ggtaaagaag cttatattgg atttaaatgc
     4501 aattccgtca ctagtaaaga aatgatcgat aagctaatcg aagcaagcca ggcaggggtt
     4561 aaaattgata tgattgttcg tggtatatgt tgtattttac ctggtgttga gggattaact
     4621 gataatatta gaataatttc tattgttggt aggtacttgg aacattcacg tatttacatc
     4681 tttggtaaag gcagtgctca aaaagtatac atttcttctg ctgatttaat gacaagaaat
     4741 ttatcacgtc gtgttgaagt cgctgcccca attttagatg aaaaattaag aaaacgaatt
     4801 attacaatgt ttaatacaat gttgaaagat aatgttaaag catgccgctt attagaagat
     4861 ggtacttaca aacgagtaaa aaataaagag ccagaattga atagtcagga atattttttc
     4921 aaataattgg caatataaat aataataaag gagtgattat atgcgggtag gtattttaac
     4981 aagtggtggt gattgccaag cactaaatgt aacaatgcgt ggtctagcaa aaactttata
     5041 tcgaaatgta aacgatatag aaattattgg ctttcttcaa ggctataaag ggttaatgta
     5101 tgaagattat aaaattatga agcccaagga tttttctgga ataattaata ttggtgggac
     5161 aattttagga acatcacgtt gtccttttaa aaaaatgcga gtaattgaag atgactttga
     5221 caaagtagct gcaatgaaaa acacatatgc aaaattaaaa ttagattgtc ttgttgtgct
     5281 aggcggaaat ggttctataa aatcagctaa catgctctct caagaaggat tgaatgttat
     5341 agcgctacct aaaacaatcg ataatgatac atggggaact gattatactt ttggctatca
     5401 gtctgcaatt gatattgcaa ccacatacct tgatcaaatt catactaccg ctgccagcca
     5461 caaccgtgtt tttgtaattg aagtaatggg acataaagtt ggccatattt gtttgtcagc
     5521 aggaattgct tcaggcgctg atgtaatttt gttaccagaa attccttacg atattaaaga
     5581 agtagctaag gctattaaaa ctagagaaaa aaatgggaaa aaattcagta ttatcgcttg
     5641 tgcggaaggc gctttatcca aagaagaagc aacactatca aaaaaagagt ataaagctaa
     5701 agttaaagcc cgcaatggtc aatcagttgt ttacgagatt gcagcagaat tagaaaaata
     5761 tattgatagc gaaattcgtg tgtcatgtat tggtcatgcc cagcgcggag gacagccatg
     5821 tccatacgat cgaatgatct caactcaatt tggggtagct ggagcccgtt tagttatggg
     5881 cggagattat ggcaaattag ttatcttaaa aaataatgga gttactgcta ttcccttagt
     5941 tgaaagtgct ggtaaactaa aatatgttga tgctgtagga gcaaaagtta aagacgctaa
     6001 gttactaggt atctcatttg gtgattaaaa agtctaaata tagattaatt tctatatcta
     6061 gactttttta ctttaattca ctagttacat caatgacttc aactaatgaa aattgctcat
     6121 cattaaattg atactttaaa acaacacaat tgggcatatc attaggtagc gatacttgct
     6181 gattttgttc ccatcttttc gcaaaacaat agcaagaaag accgtgtgag actattaaca
     6241 ctgtttcttg atcagtattt ttcataagtt ctaaaacagt tgttgaaaca cgttcactta
     6301 cttcattttc actttcacca ccaaatttct taaagaaatc gccaaatgga aaagggggat
     6361 caagatcatc gctttctcct tcataaatac caaaattcca ttcttttaaa cctttaagcc
     6421 gctgatatgg gaggctagtt acaatttcta gagtatcaca agctctttct gaagatgatg
     6481 cataagcacc atcaaaaaca atctcattat tctccaaata ctcttttaca catcttgctt
     6541 gtctttttcc cagttctgtc aaaggtgaat cacaccaccc ctgcattaac cttttttgat
     6601 taaataacgt ttgtccatga cgcatcatat ataatgtttt catattttga cttctggtat
     6661 attgtaatta acttatttaa ccgtcaattg tatacgcctc ttcctttcat tagatttcat
     6721 ttagaatcga attgtgaata ggtacttttg tttttgcctt ctgtagcccg actacctgga
     6781 aaaggttaat accacatctt tgtatcttaa ttgtaatcag ttagataagt ttttgaactt
     6841 gtatttcgca ccaggtcttt gatactctgt ttgtggtttg atcacatttc attaccttat
     6901 ataaagaaaa gatgtgtttt ccatatttta cactggtatc gatgttgaca aatttagatg
     6961 agactagcga tatccataac ttcagctcta ctgtcaaagt catagaattt gtaggtatag
     7021 accccggtac ttatcagtca atttctcgaa cagcacagtc aaaacgtggt tcgcgctatt
     7081 aagaaaaagc tatatcaatg cattctgcca gtctgcaagt acaatctcac tttcaataag
     7141 tattataaac taaaacgact gtaaggtaaa ttccacagat gtgcccagga ccactcagta
     7201 cgaaaactta caagagtaat ctataaatta ttgtctgaaa acatatagtt tgataaaaac
     7261 tgctcattta aaaatgctga acttaatatt tttaaaaagc tgcatgcagt agtagttttc
     7321 agcgtaacca aaattcactt ttcaaagaac taaaaaatat tttagctaat tatcaaaaat
     7381 tacttgattt atatatagtt aacttttctt gatcatttac aaccgataaa cttgtgtatc
     7441 taaaaaaata cagtatactt aaattatata
//
LOCUS       DAOMTA010000035         7441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_128_length_7441_cov_2.305578, whole genome shotgun sequence.
ACCESSION   DAOMTA010000035 DAOMTA010000000
VERSION     DAOMTA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128_length_7441_cov_2.305578"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..787)
                     /locus_tag="PLK68_01765"
     CDS             complement(176..787)
                     /locus_tag="PLK68_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89167.1"
                     /translation="MKSFLPLSDKSLIIETPPVKQKKLRKNNGWWPFLLEIILYYFFW
                     AIFSFLWPTGFIWSNVFAFILTVIAINLFRLFKSRYLETAVVLLLFIFVAMVVVGFIE
                     RLSTDDSTAVTASANIQEHGPETIYLPGKKYGRRMSRITFPEGQNFRVTVSGAPVMFF
                     GYYPKERYKMTQKHGTYAYISTRCDHLDFRSLTNRSSVVTIDW"
     gene            complement(790..>1137)
                     /locus_tag="PLK68_01770"
     CDS             complement(790..>1137)
                     /locus_tag="PLK68_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89168.1"
                     /translation="KGIKDAPEVEANRLEVETKKRQGELLKIQLENQAFLEEIAQSNS
                     LIYQQEIKTIMETSHCSAKDAMDFLNRQKTLDTVEKAAATGHLTYMDSSNSGNLTQGV
                     SLGYGFNVGKNTK"
     assembly_gap    1138..1154
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1155..1802)
                     /locus_tag="PLK68_01775"
     CDS             complement(<1155..1802)
                     /locus_tag="PLK68_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89169.1"
                     /translation="MLKTSFFSQWLIFCLVVISLSSVAIVFIVKYTGLFWLYWFMIFS
                     PLICAGIIFNSREIVNQTKVVIYEWRGRRMRPFLPGIYFPFRFFSFLSEGIEVPTNLL
                     VLHIFSGSRDGLADIKKYGSPDNLLPKKGGAIRLGYDITCRIINPTKFVYSYYSEAYT
                     QISNLVEKEVTVWVNNQEAEFVYNNFTQENWQELVIKKLASLVEEQMGVILMSFIP"
     gene            complement(1903..2361)
                     /locus_tag="PLK68_01780"
     CDS             complement(1903..2361)
                     /locus_tag="PLK68_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89170.1"
                     /translation="MLLCVCYVNTYSQSIEYIPPKHINYSNIHKSVSVQKIRVNLKRY
                     VGTYVIPAVNEEDEQYPPAFEEGQIIFKLIKGKLIGFAEQRILPMRGLMKTKIEVLSL
                     NHNGIGKYKSSWKNFDRYDNPNTSGKGLSGTIKFSSDRVKFMDYSCRKIR"
     gene            complement(2506..4722)
                     /locus_tag="PLK68_01785"
     CDS             complement(2506..4722)
                     /locus_tag="PLK68_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89171.1"
                     /translation="MYKYNFILKRIFAFLALFLTLSVTINAQDNDIDIADFTHRADAG
                     DADAQYELGKYYYVSNKDKDSEGAEGMAKAFKYASMSANQKNAAGEYLLSLCYLDGYN
                     EDHSKSMEWLQKAADQGYAEAQEILGFYYDFGDDGRVAQNYSKAVEWYQKAANQGNVD
                     AQRHLGICYECGTGVARNYANAYYWFKKAADQGTFIAELDHNDFLEKANSDKLSINTS
                     DPSKPLVFDIADLISQAAAGDAGAADAQYKLGKCYYEGNNVEKNGSKAFKYASMSANQ
                     RNAAGENLLGSCYCNGIGVAQNYFKAVEWYQKAADQSLDKAQYNLGCCYDEGEGVVQN
                     YADAYYWYKKAADQGNKAAIEKCNDADFLAKVKNSNEDIESNGCVAPTISYKDWELKD
                     KIYDTEINEQLLPEEKINNDSIIRKINETYYNNINSAGYTNLTSIKIKQSDINSTVFP
                     IELVSFEDGYTSSLWLDPPSNLKKFYQRESGLISFDFMSSYELTNFTAEQINMITPSA
                     FVSSIKSNQLYIGENEISGLMFDNIPEISCISDSIKDMNALIIASNRDNFIAKLLKVR
                     NKYIIANTLYINTKFELKGNAYFDISNYDINNQLLYIYLSIDYWDCKFKIMQGLYIAT
                     ITVSVPLKEATQLFVPNEKFYTPISFTVQPGASRKGLGITGGGVSCWKVPNMIIVKEP
                     QITVFTKSNYACKFNIVGMKGKMWSDGLNTYRWDLHKIDLPSKECERDVNGIFLKQ"
     gene            4960..5290
                     /locus_tag="PLK68_01790"
                     /pseudo
     CDS             4960..5290
                     /locus_tag="PLK68_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5856..6047
                     /locus_tag="PLK68_01795"
     CDS             5856..6047
                     /locus_tag="PLK68_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89172.1"
                     /translation="MFEGEIDLADNARYQIEAKKPYFVVESKSFNSINELDFKLKETI
                     DNFLQIKVSVEHLISNGQK"
     gene            6089..7058
                     /locus_tag="PLK68_01800"
                     /pseudo
     CDS             6089..7058
                     /locus_tag="PLK68_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         2416 a         1333 c         1194 g         2481 t
ORIGIN      
        1 tatttgaagc tccgtattat ctgtgcagtt gtcaatgcag ttgcaaagat acagaaaatt
       61 tagctaatca caattaaatt gttaatgttc tgttacgata aggctatatt agcacaataa
      121 ttattattta tcaactaaaa aggatagatg attaccacct atccttattt ataaattacc
      181 aatcgattgt caccactgaa gatctattcg tcagcgaacg aaaatcgaga tgatcacaac
      241 gggtactgat ataagcataa gtcccgtgct tttgtgtcat tttataacgt tctttcggat
      301 aataaccgaa gaacattact ggtgcgccgc taacagtgac gcgaaagttc tgcccttctg
      361 gaaaagtaat ccgtgacatc cgtctaccat atttcttccc tggaagatag atcgtttccg
      421 gaccatgctc ttgaatatta gcgctcgcgg ttacggctgt cgaatcatcc gtcgacaatc
      481 gctcgatgaa tccaaccacc accatcgcca caaagataaa gagcaacagg acaacggctg
      541 tttctagata tctagattta aacaaccgga acagattaat cgcgatcacc gtcaagataa
      601 aagcgaacac attactccaa ataaaaccag tcggccagag aaaagaaaag atcgcccaga
      661 agaaataata caagattatt tctagcagaa acggccacca accattgttt ttccttagtt
      721 ttttttgttt aaccggaggt gtttctatga ttaaagactt atccgataat ggtaaaaaac
      781 ttttcatgac tatttagtat ttttaccaac gttaaagcca tatcctaaag aaacaccttg
      841 agttaagttt ccgctattgg aactatccat gtaagtgaga tgtccggtag ccgccgcttt
      901 ttcaaccgta tcaagggttt tttggcgatt cagaaaatcc attgcgtctt tggctgaaca
      961 gtgagatgtt tccataattg tcttaatttc ttgctggtaa attagactgt ttgattgtgc
     1021 aatctcctca agaaatgcct gattttctaa ttggattttc aaaagttcgc cctggcgttt
     1081 tttcgtttca acttccagac gattcgcctc cacttcggga gcatccttga tacctttnnn
     1141 nnnnnnnnnn nnnnaggaat aaaagacatt aaaattacgc ccatttgttc ctcgaccaat
     1201 gatgccaact ttttgatgac taattcttgc caattttctt gagtaaaatt gttatagaca
     1261 aactccgctt cttgattatt cacccaaacc gtcacttctt tttccactaa attactgatt
     1321 tgagtataag cttcgctata ataactataa acaaattttg tgggattaat aatccgacac
     1381 gtaatatcat accctaatct aatagctcca ccttttttag gtagaaggtt gtctgggcta
     1441 ccatattttt ttatatccgc taaaccgtcg cgcgaaccgc taaaaatatg cagtactaat
     1501 aaattagtcg gcacttctat tccctcggat aaaaaactga agaaccgaaa agggaaataa
     1561 ataccaggca aaaacggtct catccgtcgc ccacgccatt cgtaaataac cacctttgtc
     1621 tggttaacaa tttcacggct gttgaagatg atgccagcgc aaattaatgg tgagaaaatc
     1681 ataaaccagt ataaccagaa aagaccggta tacttaacaa taaacacaat agccaccgaa
     1741 cttaagctga tgaccaccaa gcaaaagata agccattggc taaaaaaact agtttttagc
     1801 atcggtcttt catctctgct ctctgtttca aaatttctca ttttttataa tggctatata
     1861 atgttacgcc tcctcatatt attatttatg atctctgatt ttttaacgga tcttgcgaca
     1921 tgaataatcc ataaatttaa ctctatcact tgaaaatttt atagttccac tcaatccttt
     1981 accactcgta ttgggattat cataacgatc aaagtttttc catgatgact tatattttcc
     2041 aatcccatta tgattaagtg aaagaacttc aatcttagtc ttcatcaacc ctcgcatcgg
     2101 taatatgcgc tgctcagcaa aaccaatcag ttttccttta attaatttaa atatgatttg
     2161 tccctcttca aaagctggtg gatattgttc atcttcttca tttactgctg gaatcacata
     2221 agtgcctaca tatcttttta aattaactct tatcttttga actgatactg acttatgaat
     2281 attagaatag ttgatatgct ttggtggaat atactcgatt gattggctat aagtattaac
     2341 ataacataca cacagaagca ctactcctaa aacgatattt agattcttca ttatataaat
     2401 atttgtgcca acctattctc actggaaggc tttttataaa gaggcgtgag aactttgata
     2461 tcattaaaat aatacgcttc atgattatat ttggtgatat tatttttatt gttttaaaaa
     2521 tataccatta acatcccttt cacattcttt gcttggtaga tcgattttat gaagatccca
     2581 tctataggta ttgagcccat cggaccacat tttacctttc attccaacaa tattgaattt
     2641 acatgcataa ttgctctttg taaatactgt gatttgaggt tcttttacta ttatcatgtt
     2701 tggtactttc caacaactta cacctcctcc tgttattcct aatcctttac gagatgctcc
     2761 tggttgtacc gtaaaagata taggtgtgta aaatttttca ttcggtacga ataactgggt
     2821 ggcttctttg agaggaacag atactgtaat tgtagctata taaagtcctt gcataatttt
     2881 aaatttgcag tcccagtaat caatagatag ataaatatat aaaagctgat tgtttatgtc
     2941 ataattagat atatcaaaat atgcattccc cttaagttca aattttgtat ttatatataa
     3001 tgtgttagca ataatgtatt tatttcttac ctttagcagc tttgctatga aattatctct
     3061 gttcgaagct attatcaatg cattcatatc tttgattgag tcagaaatac agctaatttc
     3121 agggatatta tcaaacatta aaccggatat ttcattttca cctatgtata attggtttga
     3181 cttaatactt gaaacaaatg cagaaggagt aatcatgttg atttgttctg ctgtaaaatt
     3241 ggtaagttca taagatgaca taaaatcgaa tgatattaaa cccgattccc tttgataaaa
     3301 ttttttcaaa tttgatggcg gatcgagcca aagagaactc gtatatccgt cttcaaaaga
     3361 tactaactca ataggaaata cagtagaatt tatatcactt tgtttgattt ttatactggt
     3421 taagtttgta taaccggctg aattgatatt attataatat gtttcgttaa ttttacggat
     3481 gatcgaatcg ttattgattt tttcttctgg caataattgt tcattgattt cagtatcata
     3541 tattttatct ttcaattccc agtctttata agatatagtt ggagcaacgc atccattaga
     3601 ttcgatgtct tcgttactat ttttaacctt agcgagaaaa tcagcatcgt tacatttctc
     3661 tatagctgct ttattgcctt ggtcagccgc ttttttgtac caataataag cgtcggcata
     3721 attttgtacc acaccttcac cctcatcata acaacaacca agattgtatt gtgctttgtc
     3781 aaggctttga tccgcagctt tttgatacca ttccacagct ttgaaataat tttgtgcaac
     3841 tccaatccca ttgcaatagc atgaacctaa tagattctct cctgccgcat ttctttggtt
     3901 agctgacatt gatgcatatt taaaagcttt tgagccgttt ttttccacat tattaccctc
     3961 gtaataacat ttgcccaatt tatattgagc atcagcggca ccagcatcac cagcagcagc
     4021 ctggctaatt aaatccgcta tatcaaatac tagtggttta cttggatcag acgtattaat
     4081 ggacaactta tcactattag ctttctcgag aaaatcatta tgatcgagtt cagctatgaa
     4141 agtaccctga tcagctgctt ttttaaacca ataataagca ttggcataat ttcgtgccac
     4201 accagtacca cattcataac atatacctag atgacgttgc gcatcaacat tcccttgatt
     4261 ggcggctttt tgataccatt ccacagcttt ggaataattt tgtgcaactc tgccatcgtc
     4321 accaaaatca tagtaaaagc caagaatttc ttgtgcttct gcataaccct gatcagcagc
     4381 tttttgaagc cattccatac ttttagaatg atcttcattg taaccatcta aatagcataa
     4441 acttaacaaa tattctcctg ctgcattctt ttggttagca gacatagatg catatttaaa
     4501 agcttttgcc atgccttctg ccccttcact atctttatct ttattagaaa cgtaataata
     4561 tttacccaat tcatattgag catcagcgtc acctgcgtct gcgcggtgag taaaatctgc
     4621 tatatcaata tcattatctt gcgcatttat tgttaccgat aaggtaagaa ataatgcaag
     4681 aaatgcgaat atgcgtttta aaataaaatt atatttatac atagtagcta ttttatatta
     4741 atcaagaaat tatactggta tctcattaac acaagacgat atttagggtc tgctaattta
     4801 tttacagtgt agcatatagt aatttgcaag atttcgtaca aagatgattc tgtcgaattt
     4861 tgtctttttg agagccatca gagcaaaata tatctccaaa taaaatatat aaagaaactc
     4921 agacgtatag tttattatgc tcagacattg aatggagctt ttatctatct taccaataat
     4981 tttgagatat ctgctgaaca ggttgaactc ctctataaat atcgttggca agtagaatcc
     5041 tttttcaaat ggataaagtt gcatcttaaa atcaaattct tttggggtac aaccaagagc
     5101 gctgtacgta ttcagatatt tacgaccagc actgcatatt gtatggtggc tattgttgat
     5161 aatgatcttc aattgcacag aaacatctac gaagtgttga gaatttttaa gcacttcact
     5221 tttggataag actccgataa aggaactctt tctcaaacaa tgtctgatgt ccttgaggat
     5281 ggtcaattaa ctcttaaagt gatttttgtt ttgtaattta ctcattttct atcatgtgcg
     5341 caagtgtatt tatataaaat aactatattt gacatttatt atttatatcc tgtttagttg
     5401 ataagtttat aatcaaaatc attagtgtcc cgttaaaatc gagacgagtt aaaaattaaa
     5461 taaacagctc cagagtaagg ggatcaagtt gctctttgac atcattgaaa ttagtattat
     5521 taaacaggtc acgtaaatgt gttttgtctg ttccttacgg acaaaacacc aattagcgaa
     5581 ttatttaaca agactatccc cgagaaaatg tctaatgact attatatacc gagtctattc
     5641 ggtcaagaag atttctaagc ggaacagcag tgatattatt acatgaaaca taattatacc
     5701 atcgaaatat aaaaaaccgg ttattcggct taaagaggtt gcaaaagcga accaaatact
     5761 tgacgaaaat gataaaaggt tggaatatta tttttcaata cacatacaat aaaattcctt
     5821 atacatactg ttggaggaat tggggacata ttgatatgtt tgaaggagaa attgacttgg
     5881 cagacaatgc tcggtatcag atagaagcta aaaagccata ttttgtagta gagagtaaat
     5941 ctttcaacag tattaatgaa ttagatttca aattaaagga aaccatagat aactttcttc
     6001 agatcaaagt tagtgttgaa catttgatat ccaatggtca aaaataacac aataacccgc
     6061 tggtggaatt aaataaaaaa gaatggaaat gaaaaagttg cacatatcgt tgattattag
     6121 tatattagat gtaaaaaaac ttttaatata taatcgtcgt atgcacaact tatatacaat
     6181 attcgttaaa atacttgaga taaacaaaaa gttcgcgatt aatttagtgg ataaaagagg
     6241 aaacatacct cgtcgaggtg tagttcccaa attttcggat ttggaagtta tttcattaag
     6301 tatgacagca gaatctatag ggatagacag tgagaattac ttattttcca agttagagga
     6361 atacaaaatg gatataccaa accttagatc acgtcgccaa tacaatgacc gcaggaagat
     6421 tacttcgtca ttatgtaaca agctgcgtga aaagatagtt tcatatatag atagcggtga
     6481 agatatgtat tgtatagatt ccaagccgat agaagtttgc cgtccggtta gagctaaacg
     6541 ctatagaatg ggtaaagata attatgagaa agctcccaat acaggctatt gtgcatctca
     6601 aggcaaatat tattatggat atacttcacg cgatatgtgg attaagtggc gttattcatt
     6661 cctttgacat gactaaagcc agtgtacatg atatgaacta tttgcaagac gtgaaatacg
     6721 aatatcatga ttgcagtatc tttggagaca gaggatatat tggtacagac gtccaacttg
     6781 atttgtttga gacagcacac attaaaatgg aagttcctta cagattaaat caaaaagatt
     6841 ggaaaccgac atttattcct tttgctaagg caagaaaaag gatagaaact gtattttcgc
     6901 aattatgcga tcaatttatg attgttagaa attatgcaaa acaaactgaa ggattattta
     6961 ccaggattat cagtaaaatt actgctctta cattctttca gtatataaac aaaattaata
     7021 ataaacctat tgacaaagtt aaatatgctc ttctttaatt ctgccaacgg gtcacaatag
     7081 tatattttta tgcttcaata catttctagc acctactatt acgaatatcc taaagtattg
     7141 agagaaggat acgtaatggc ttttgttgtg taataatagt atttggtatg ggcgagctta
     7201 caaattattc cgcggttttg gtgaatgaaa ttctttatcc accaataatt tgcttacctt
     7261 tacaccgcta tttagaggta gagttaaaga ccttcacttt caagttattg tcccaagctc
     7321 ttctctttaa gtccgcgtat gaccccaaca tcaaaatggg agatactgcg attgaaaact
     7381 atttcataag atataaaagg taaagatctc tcttacaaag ggtcatgtat tatgatctaa
     7441 c
//
LOCUS       DAOMTA010000036         7354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_129_length_7354_cov_3.032607, whole genome shotgun sequence.
ACCESSION   DAOMTA010000036 DAOMTA010000000
VERSION     DAOMTA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129_length_7354_cov_3.032607"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLK68_01805"
     CDS             complement(<1..255)
                     /locus_tag="PLK68_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89173.1"
                     /translation="MNKQFDIFSKMKITEMCKTIHDMTYTYINPETKQPTDVPAKHYQ
                     DILKQPVEVMVEDQVKKQFLNIMYKQLKNLKEEEPHLFYQS"
     gene            complement(248..565)
                     /locus_tag="PLK68_01810"
     CDS             complement(248..565)
                     /locus_tag="PLK68_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89174.1"
                     /translation="MNNLDPVIINYKNLTPSDKRIIQAKLSVLGLIGNAKDEYKEDIK
                     GKTALTRLMYESAIETADELESKIKEAIILNMAEMIDGYDYVVDDQDTDDYFYGLECD
                     DNE"
     gene            complement(558..1730)
                     /locus_tag="PLK68_01815"
     CDS             complement(558..1730)
                     /locus_tag="PLK68_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRM89175.1"
                     /translation="MEDNKINTFLLNRGIDSSVPLLNLEEEVLGDIKQHDIFTETSFD
                     MLKIKNQIRGQKKEMMMRNCIVENVNVRALTISGKCTFYNCTFNNVVFTTCSLPNCCF
                     YHCTFNDSAIVESTLSSVLFVNCNDLEEVDFKGNNLSNMKVIGSNLIAIALENQGNKV
                     EGAYIREESFMTGVEEKVEEHKEVEITTRQTVEKITYKNESFGLKELKNTLFSECVFI
                     SCEFDKNMQLDASTDFINCTFKKMDLNIFIFQKVIFDYSTFEDVFMSSRFLHCSFVGC
                     QFKSLSFSDDVAFTSCNFALSNAFEVFDMNKHHMTNIELNRKEVEEDEQLNILLDALY
                     TLLKDRDKELQRSLRLLDRDAVTKDIMADYLMNLNDRQYIEFISEVGTRRRDEEDE"
     gene            complement(1720..3657)
                     /locus_tag="PLK68_01820"
     CDS             complement(1720..3657)
                     /locus_tag="PLK68_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM89176.1"
                     /translation="MDKKKILISWIVSLFIVYFVGMQLAYLFSGNVGITVNPKYLFIN
                     GSYFIGLTMFTLIVPLFLLMNKMNDKDTSDKDNYSSLTDMDYYKKQMTPLYFDKQCHI
                     TTSPMDLRIKKFNSYLNMKLPTKSAHDKSGLIFMSYKDHVYVDDSDSHSLIIGSSGSG
                     KSFSFMLPMLCLMAMTGESGLTVDIKGELSEKTAELFKSQGYDVYFIDFIDPDNSDCW
                     NPLYLGASEYVRQLAIQNEQLERYHKMKDEIDKEYQTLYGDGAIPNYESYFGTNDAGD
                     YIYDKYRQEFRTTADFSSAQEYFRDVVQAITSGKKEANEDNFWNEEAGRVLEGYIHLL
                     AESGNIDVINIPAVARVMLEGDEVKKKTSRGNVSWLEYYLQNHKTNSDLSKEKLMSYV
                     DSAEQTRKSSRNVLTKHLNSIVTNDAIKKMLSNNDIDLENIGNKKTMIYLKVHDEKQT
                     YYPLVTLFIKQLWQSLVKTARSSPNLRLPIPFHIMFDEMGQFPAFDEITNVLTAGRSR
                     GVRLTAVVQGYDQLDNAYGKNTAKTIKNNAKNTVYLLSGDYETLDELSKRCGSHYITR
                     NGRKELERVITPDRLQNFKFGEALILRQREKAFITKVIPFNQYVFYPSLVDYKLRYVA
                     KKEPKYFSIMDDIKEKENSGR"
     gene            complement(3718..4431)
                     /locus_tag="PLK68_01825"
     CDS             complement(3718..4431)
                     /locus_tag="PLK68_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRM89177.1"
                     /translation="MLYIALVAICAGVVQGVTGFGAGIVMMMVLPSIIGSIPSSAGIS
                     TAICTILSLQMAYTYRKHIDFKKIIYPAVLFITIGTTAMYFSTMVNQALMKKVFGAFL
                     IILSIYYLFFNKNRERKKLSLTVSIFCIVISALCDGLFGVGGPLMVLYFLSTTHNTHQ
                     YLGTIQTFFFMTCIYNTCFRIFRGILLPEHLVFVAIGMACIVVGGFIGNKIVTKLDGA
                     LIKKLTYVVIGLAGIINII"
     gene            complement(4538..5275)
                     /locus_tag="PLK68_01830"
     CDS             complement(4538..5275)
                     /locus_tag="PLK68_01830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM89178.1"
                     /translation="MTNKVVLVTGGTRGIGLATVKKYLSEGYKVVVVDLFESDELKEL
                     ENVTFIATDISKEEDCKNAVTVATKKYGHIDILVNVAGIIGERKAFTDIDLKNVMKVI
                     EVNLMGTIYMAHFVSKEMIKRNSGVIVNIGSIDGFMANTEAIGYHASKGGVKMATQAM
                     ARELSPYGIRVVSVAPGWVRTGMIDEPIAKIGGALHMKGRIIEPEEIANVIYLVSLPE
                     ASAINGSTVCADDGYLSFKGVDGFKAY"
     gene            5404..6261
                     /locus_tag="PLK68_01835"
     CDS             5404..6261
                     /locus_tag="PLK68_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM89179.1"
                     /translation="MTDRQLELFIIAASCKSFSKAANKSYVSVSSLVQQIDALEKDLG
                     FELFIRNNRGISLSENGKKFHEAATKILEIYNSTIEECKKLYKIKIAIAPEQIPDFLL
                     KACKTFNIDYPDCKIELIAKPYNEHLNAIENLECDCSIIAEPSKKFLKDLHFIPLYKD
                     TYSFMVPFQHPLASKEIIELSDLNNCTIICGYYPYLKYTFENGLKESNAKIMHSHDEY
                     NFQLKADNMFSQNIMVFHTLWKESHKNILKVVPSSIPAGDIGIVCKQKSIINSFLPYI
                     KQAIKDVNS"
     gene            complement(6353..>7354)
                     /gene="mobL"
                     /locus_tag="PLK68_01840"
     CDS             complement(6353..>7354)
                     /gene="mobL"
                     /locus_tag="PLK68_01840"
                     /inference="COORDINATES: protein motif:HMM:NF037847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HRM89180.1"
                     /translation="YTGSHSVGTYSSSGELNTKEERDALKKEIDQCFHKDGNLCWDYV
                     ISLENDKEAYNLGLENTEQWLSAVKEFLPKMLKEYHIDYNNTLWWFDLHRNTEHPHIH
                     LVFMEKHQTRTRGKLTPREMKNVKRHIYSSLSARAKLKERTGKDYRTFFQEKDMSLKE
                     LMTSIEKKDLKKNPKLKGLYKVLPKTGRLQYNSYNMKQYQPMIKSIIDDLIQSDDELN
                     KTFNEYISKLDQLEEVMNEEGNDIATIKEAELEKFYSKVGNYILQNFKKTEYVEESKK
                     DGNKVNLPYKPKKTYKKKYLTETKLKGYIHKLANEQQSEIDKAIEEYNRRMEEQLLV"
BASE COUNT         2354 a         1361 c          922 g         2717 t
ORIGIN      
        1 tgactgatag aaaagatgtg gctcttcttc ctttagattc tttagctgtt tatacatgat
       61 atttaagaat tgcttcttta cctgatcttc aaccataact tcaactggtt gttttaaaat
      121 atcctggtaa tgttttgcag gtacatctgt aggctgcttt gtttcaggat tgatataggt
      181 ataagtcata tcatgaatgg tcttacacat ttctgtaatc ttcatcttag aaaaaatatc
      241 aaactgctta ttcattgtca tcacactcca atccgtagaa ataatcatct gtatcctgat
      301 catcaactac ataatcatag ccatcaatca tttctgccat gtttaaaata atagcttctt
      361 tgattttgct ttctaattca tctgctgttt caattgcact ttcatacatt aatcttgtta
      421 aggcagtttt acctttgatg tcttctttat attcatcctt cgcatttcca attaaaccta
      481 atacacttaa cttagcctgg atgattcttt tgtcactagg cgttaagttc ttataattaa
      541 tgattactgg atctaaatta ttcatcttct tcatcccttc ttcttgttcc gacttcactg
      601 ataaactcta tatactgacg atcattcaag ttcattaaat aatcagccat gatatccttt
      661 gttactgcat ctctatcaag tagtcttaaa cttctctgta attctttatc tctatcttta
      721 agtaacgtat acaatgcatc taaaagaata ttcagctgtt cgtcttcttc aacttctttt
      781 ctatttaatt caatgtttgt catatgatgt ttgttcatat caaatacttc aaacgcattc
      841 gataaagcaa agttacatga tgtaaaagca acatcatcac tgaaacttaa tgacttaaac
      901 tgacatccaa caaagctaca atgtaagaat ctagatgaca taaagacatc ttcaaatgtt
      961 gagtaatcaa agatgacttt ttggaagatg aaaatattta aatccatttt cttgaaagta
     1021 cagtttataa agtctgtact ggcatccaac tgcatattct tatcaaactc acaggaaata
     1081 aatacacatt cactaaacaa ggtattcttt aattccttta atccaaatga ttcattttta
     1141 taggtaatct tttctactgt ctgtcttgtt gtaatttcaa cttctttatg ttcttccacc
     1201 ttttcttcta ctcctgtcat aaatgattct tctctgatat aagcaccttc aactttattt
     1261 ccctgatttt ctaatgcaat agcaatcaaa ttactaccta tcactttcat attgcttagg
     1321 ttattgcctt taaaatcaac ttcttctaaa tcattacagt ttacgaatag tactgaactc
     1381 aatgttgatt ccacaatcgc tgaatcatta aaagtacagt gataaaagca acagttaggt
     1441 aacgaacatg ttgtaaacac gacattatta aacgtgcagt tatagaaggt acatttacca
     1501 gagattgtta atgccctgac attaacattt tcaacaatgc agtttcgcat catcatctct
     1561 ttcttttgac ctctgatctg atttttaatc tttaacatat caaaagaagt ctcagtaaaa
     1621 atatcatgtt gtttgatatc ccctaaaact tcttcttcca aatttaaaag aggtactgac
     1681 gaatcaatac ctcgatttaa taaaaacgta ttaattttat tatcttccac tattttcctt
     1741 ctcctttata tcatccataa tactgaaata ttttggttct ttctttgcga catatctaag
     1801 tttgtaatca acaagtgacg gatagaatac atactgatta aatgggatga ccttagtgat
     1861 aaaagctttt tctctttgtc taagaattaa tgcttcacca aatttaaagt tttgtaatct
     1921 atcaggtgtt attacccttt ctaattcttt tctaccattc cttgtaatgt aatgtgatcc
     1981 acatcgcttg gataattcat ctaatgtttc ataatcacca cttaacagat aaacagtatt
     2041 ctttgcattg ttcttgattg tcttagcagt atttttgcca taggcattat ctaactgatc
     2101 atatccctga acaacagcag tcagtcttac acctcttgaa cgacctgcag ttaatacatt
     2161 cgtgatttca tcaaatgctg gaaactggcc catttcatcg aacataatat gaaatggtat
     2221 aggaagtctt agatttggtg atgatcttgc cgtcttaaca aggctttgcc ataactgttt
     2281 aataaataat gtaactaatg gatagtaagt ctgtttttca tcatgaacct tcagataaat
     2341 cattgtcttt ttattaccaa tattctctag atcaatatca ttattagaca gcatcttttt
     2401 aatcgcatca ttggtaacaa tactgtttag atgtttagta aggacattac gtgatgactt
     2461 tcttgtctgt tcagcactgt ctacataaga catcagcttt tccttactta gatcactgtt
     2521 tgtcttatga ttttgcaagt aatactctaa ccaggaaaca ttacctcgtg atgttttctt
     2581 tttgacttca tcaccttcta gcataactct agctactgca ggaatattga tgacatcaat
     2641 attgccactt tctgcaagta agtgaatata tccttctaat acacgacctg cttcttcatt
     2701 ccagaagtta tcttcatttg cttctttttt gcctgaagta atagcttgta caacatctct
     2761 gaaatattcc tgagctgatg aaaaatcagc tgttgttctg aattcttgtc gatatttgtc
     2821 gtaaatataa tcacctgcat catttgtacc aaaataagat tcataatttg gtatagcacc
     2881 atcaccatat aaagtctgat attctttatc tatttcatct ttcatcttgt gatagcgttc
     2941 taattgttca ttttgaatag ccaactgtct tacatattca cttgcaccta aatacaatgg
     3001 attccagcaa tcactgttat ctggatcaat gaaatcaata aagtaaacat cataaccttg
     3061 tgatttaaac agttctgcag tcttttctga taattcacct ttaatgtcta ccgttaatcc
     3121 cgattcacct gtcattgcca ttagacataa cattggcagc ataaaactaa atgatttacc
     3181 agaaccagaa gaaccaataa tcaagctatg tgaatcactg tcatcaacat agacatgatc
     3241 tttataagac ataaaaataa gtccactctt atcatgagca gacttcgttg gtaatttcat
     3301 attaagataa ctattaaact tctttattct taaatccatg ggtgatgttg taatgtgaca
     3361 ttgcttatca aaatataatg gcgtcatctg tttcttatag taatccatat ctgttaaaga
     3421 tgaataatta tctttgtcac ttgtatcttt atcattcatt ttattcatca gcaaaaacaa
     3481 aggaactatt aatgtaaaca tcgtcaatcc tataaaatat gaaccattaa taaataaata
     3541 ctttggattc actgtaattc caacattacc tgaaaataag tatgcaagct gcattcctac
     3601 aaaataaaca ataaataaac taactatcca ggatatcaat attttcttct tatccatgca
     3661 tctccttcct acaaggtaaa tttaccttgt aaataaaaaa tgccttttca ggcattttta
     3721 tatgatattt ataattccag ctaatccaat aacaacatag gttaattttt taatcaacgc
     3781 accatctaat ttagtaacaa tcttatttcc aataaatcca ccaacaacta tacatgccat
     3841 accaattgcc acgaatacga gatgttctgg taataaaatc cctctaaata ttctaaaaca
     3901 tgtattatag atacatgtca tgaagaagaa tgtctgtata gttcctaaat attgatgtgt
     3961 gttatgagtt gtagagagga aatataaaac cattaaaggt cctcctaccc caaataagcc
     4021 atcacacaat gcagaaataa caatacagaa aatactgaca gtcagactca actttttacg
     4081 ttctctattt ttattgaaaa ataagtaata aatagacaga atgattaaaa aagcaccaaa
     4141 gacttttttc attaatgctt gattaaccat tgttgaaaaa tacatagcag ttgttccgat
     4201 tgtaatgaat aaaaccgctg gatatataat ctttttaaaa tctatatgtt ttctatacgt
     4261 atatgccatt tgtaaggata aaatagtaca tatagccgta gaaataccag cacttgaggg
     4321 aatggatcct ataatagatg gtaaaaccat catcataact ataccagccc caaatcctgt
     4381 aacgccttgt acaacaccgg cacagatggc tactagtgca atatataaca tctgtttaat
     4441 ctccaattct tatccatttt ctcaattcca caaatgcctc aaaaattagt attgttttgc
     4501 ctcatacatt ttggacaaat cctatcctaa attgagatta ataagcttta aatccatcta
     4561 cacctttgaa tgataaataa ccatcatcag cacaaactgt tgatccattt atagctgatg
     4621 cttcaggtaa tgaaactaaa taaataacat ttgcgatttc ttctggttca ataattctac
     4681 ctttcatatg taaagcccca ccaattttag caattggttc atcaatcatt ccggttctta
     4741 cccaaccagg tgctacagat actacgcgaa taccatatgg agataattct ctcgccattg
     4801 cttgtgtagc catcttaacg ccacctttac ttgcatgata tccaattgcc tctgtattcg
     4861 ccataaagcc atcgatagaa ccgatattaa caatcacacc actattgcgt ttaatcattt
     4921 ctttagaaac gaaatgtgcc atataaatag tacccattag gtttacttca ataactttca
     4981 taacattttt taaatctatg tcagtaaatg cttttctttc tcctataatt ccagctacat
     5041 ttactaaaat atcaatatgg ccatattttt tagtagctac agttactgca ttcttacaat
     5101 cttcttcttt tgaaatatct gttgcaataa acgttacgtt ttctaattct tttaattcat
     5161 cactttcaaa taaatctact acaactacct tatagccttc acttaaatat tttttgactg
     5221 tggctagacc aattcctctt gttccgccag ttactaatac aactttattt gtcatcttta
     5281 tacatctcct ttttttatat tatagagtat gtttagcata atcaatttac gtaaatacaa
     5341 tttcagcata aggcttttac cttaaacaaa aattatatat aatggtactt gggagtgatt
     5401 attatgaccg atagacagtt agaattattt attattgcag catcatgtaa aagtttttct
     5461 aaagctgcta acaagagcta tgtatctgtt tcttctttgg ttcaacaaat tgatgcatta
     5521 gaaaaagatc ttggattcga attatttatc cgcaacaatc gaggtataag tttaagcgaa
     5581 aatgggaaaa agttccatga agcagccact aaaattcttg aaatttataa ttcgacaatt
     5641 gaagagtgta aaaagcttta taaaattaaa attgcaattg ctccagaaca gattcctgat
     5701 ttcttactca aagcatgcaa aacttttaat atcgattatc ctgactgcaa aattgaactt
     5761 atagccaagc catataatga acatttaaat gcaattgaaa atttagaatg tgattgcagc
     5821 attattgctg aaccatcaaa aaaatttttg aaagatttgc actttattcc tttatacaaa
     5881 gatacttaca gtttcatggt tccttttcaa catccattgg catctaagga aataatagaa
     5941 ctgtctgact taaataattg tacaatcatc tgtggttatt acccttatct taaatatact
     6001 tttgaaaatg gtttaaaaga aagtaatgct aagattatgc attctcatga tgaatacaat
     6061 ttccaattaa aagcagataa tatgtttagt caaaatataa tggtatttca cactctttgg
     6121 aaagaatctc ataaaaatat tttaaaggtt gtaccttctt ctattccagc tggcgacata
     6181 ggtattgtat gtaagcaaaa atcaatcatc aactcatttt taccatatat aaaacaagct
     6241 ataaaagacg ttaattcata gttaattaca tgtcgacaca taccaagaat agcatttcgc
     6301 tattctttta ttttttgtct gtatcagtaa cagaccttct gatgtaatat tcctagacca
     6361 ataactgttc ttccattctt cgattgtatt cttcaatagc cttatcaatt tcactttgtt
     6421 gctcattggc tagtttatga atgtatcctt tgagcttggt ttcagttaaa tatttctttt
     6481 tataggtctt tttaggttta taaggtaaat ttaccttgtt accatctttc tttgattctt
     6541 ctacatattc agtcttttta aaattttgaa gtatgtagtt accaacttta gaatagaact
     6601 tttctagttc agcttcttta attgtcgcaa tatcattacc ttcttcattc atcacttctt
     6661 ctaactgatc taacttactt atatattcat taaatgtctt atttagttca tcatcacttt
     6721 gaatcagatc atcaataata gatttaatca ttggttgata ttgcttcata ttgtatgaat
     6781 tgtattgaag ccttccagtc ttaggtaata ctttatacaa accttttagc tttggatttt
     6841 tctttagatc ctttttctca attgatgtca tcagttcctt tagtgacata tctttttcct
     6901 ggaagaaagt tctataatcc ttaccagttc tttcttttag ttttgctctt gcacttaatg
     6961 atgaataaat atgtcttttt acgttcttca tttctcttgg ggtaagtttt cctctagttc
     7021 ttgtttgatg cttttccata aatacaagat ggatatgtgg atgttcagta ttacgatgaa
     7081 gatcaaacca ccataaggtg ttgttgtaat caatatgata ttccttcagc atctttggca
     7141 gaaattcttt gactgcagat aaccattgtt ctgtgttctc taatcccaaa ttatatgctt
     7201 ctttatcatt ctctaatgaa atgacataat cccagcaaag attaccatct ttatgaaaac
     7261 attgatctat ttcttttttt aaagcatcac gttcttcttt ggtatttagt tcaccacttg
     7321 aactataggt tccaacacta tgacttcctg tata
//
LOCUS       DAOMTA010000037         7260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_131_length_7260_cov_2.057321, whole genome shotgun sequence.
ACCESSION   DAOMTA010000037 DAOMTA010000000
VERSION     DAOMTA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131_length_7260_cov_2.057321"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLK68_01845"
     CDS             complement(<1..309)
                     /locus_tag="PLK68_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89181.1"
                     /translation="MDTSEEADEIWEDSFKYVDHNSAEVTAASDALKELSKHMINQNM
                     DSRVTPLQESVELKDRLNQFKTALNSGNTISDDLFTDLINEFTKLQTAAKTYRNEAGD
                     T"
     gene            complement(234..>1423)
                     /locus_tag="PLK68_01850"
     CDS             complement(234..>1423)
                     /locus_tag="PLK68_01850"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRM89182.1"
                     /translation="EIKEDQTNILVGIKGSDGYVDKHVSKNYTLKTSGLYDKLDSYLL
                     ASDNGTITVLGKDTDAAFYGITSLYHIFNQMDSYSIRNFVMEDYADVASRGFIEGYYG
                     NPWSTEDRSELMKWGGYYKLNSYFYAPKDDPKHNSKWRELYSDEEIETKIKPLAQAGN
                     ESKCRFVFALHPYMNNAIRYNSEENYQADLKVMQAKFAQVIEAGVRQIAILADDAGNV
                     GGDNYIKTLKDMTAWIKEMQKTYPDLKLTLPFCTQEYMGNGQGYYTNFPENVQIVMTG
                     GRVWGEVSNNFTTTFTNNVGRGPYMWINWPCTDNSKKHLIMGGYSTFLHPGVDPNKIQ
                     GIVLNPMQQSEPSKVAIFGNACYSWNIWQSANEADQAWTLVKKQMKFGKIHSNMLIII
                     AQK"
     assembly_gap    1424..1433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1434..1710)
                     /locus_tag="PLK68_01855"
     CDS             complement(<1434..1710)
                     /locus_tag="PLK68_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRM89183.1"
                     /translation="MRKIKKNTHKLMSMFLAVLMVFGCFSAWPTQVSAAESDYEIYPK
                     PHLMEYQSGDYVIHQNINIVYEDGIDEYTKARMNEVLAIKKINATISN"
     gene            complement(1940..2770)
                     /locus_tag="PLK68_01860"
     CDS             complement(1940..2770)
                     /locus_tag="PLK68_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89184.1"
                     /translation="MKILKVFNNNSVAAISDELGDIILTGSGIGFQKRIGDEVDESRI
                     EKTYFFKDDQQKRFEQSIETVPAIYFEITNKIVNQANKELDTDFSGEIFLAISDHISF
                     AVKRKKEEIYLPNVVLSETKVLYKKEYKVGLWALDYIEEKTGIRLDDDEAGYIALHLV
                     NFSLDNKANNATKIVTLTKEVLNVIKLSMKVDLEEDSLGYARISTHLKYLAERIFRDE
                     IDELQDTTADIREMLKEDLRLSLCINRIVKLIRDRYDYELSPDEQTYLCIHIKKNAKL
                     "
     gene            complement(2790..>4317)
                     /gene="nagE"
                     /locus_tag="PLK68_01865"
     CDS             complement(2790..>4317)
                     /gene="nagE"
                     /locus_tag="PLK68_01865"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HRM89185.1"
                     /translation="FRNINILNGKVQLNPILIGGMGMMKYMQRLGKSLMLPVSVMPIA
                     ALLKGIGYWIDPTGWGSNNAIAAFLIESGGAIIDNLPILFAVGIAIGMSQERELTVAL
                     SAVVSYMIVSRLLSPPAVSLIKGIPEAEVSAAFENSANAFVGIISGLVVAYNYKKFSK
                     VKLPDALSFFGGKRFVPIISTASMLVISLLLLIVWPFFYECFVSFGEMISKLGPVGAG
                     LYGFFNRLLIPTGLHHALNSVFWFDVAGINDIGKFWGTVSGGIKGVTGMYQAGFFPVM
                     MFGLPAAAIAIYQSARPERKKQVGTILLSAAFASFVTGVTEPLEFSFMFVAPVLYFIH
                     ALLTGIMLFVAAVFKWLAGFGFSAGLIDYILSIKAPFSNDIFMLIPLGLICAFIYYCI
                     FRFMIVRYNLMTPGRETDEDIIEIEEEEYNITLENQDYDEIAIVLIEALGGRNNVQFA
                     DSCITRLRVELGNPDLVDEQAILATGATGIIKIGKNNLQIIIGTEVQFIVDAMNDILM
                     "
     assembly_gap    4318..4340
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4733..5215
                     /locus_tag="PLK68_01870"
     CDS             4733..5215
                     /locus_tag="PLK68_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM89186.1"
                     /translation="MNFFTRLLKNKKNTVCAPACGKVIRLENIPDKAFANKMVGDGLA
                     IEITDNKLVAPCEGIISVIANTKHAFVMTLPNGLHLLVHIGINRAKPDANNFQYHVKA
                     GDYVSLGADIVTLSDNLLKVLNNKVITPIIVCNYESHPIKTFTTASSVETGKTIFTYK
                     "
     gene            complement(5346..5423)
                     /locus_tag="PLK68_01875"
     CDS             complement(5346..5423)
                     /locus_tag="PLK68_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89187.1"
                     /translation="MFAYVTGLGVVAIVGIYWLLRKKEE"
     gene            complement(5579..>7260)
                     /locus_tag="PLK68_01880"
     CDS             complement(5579..>7260)
                     /locus_tag="PLK68_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HRM89188.1"
                     /translation="KYFTYDLGQEITLNSFKAICTDSEWDYPRHGKFSVSTDGETWTE
                     IMLLGNQNENNPGEAENTDEISFVLPSHEISYNAKKVDGLNLQARYLKFEITRTKVGA
                     DKWVRFQELEINNGEFIPSQNNPTFTSSSQETRDGLFSYMVDGDISTMFIPKDANGYV
                     NYSLSSNNQVNTIKIIQNSAVISNAVVKARLFTNASAEQWVTLGTLSQAINEFVLPEN
                     TILLDIKVEWGDVIPNITELSTYKSEVTTLNVDALKALIDNKEDLSSWTAAAAATYGD
                     AYNAGKQVLESEYASQTTVDNAVRAINKAIENKVLKGDLSKLEIIIANAHTDQNNYTA
                     ASWLAYSKAIEAINKSIANGDNTSVADVDKLIANYDLANTNLVFNPSNQEEAIITIEG
                     ENDFVASVTNPEKLYTINSWKMYLEAKAKVEQLIIDNQSTPVHPDEFAKAISELAAAK
                     EKLVVVGDLKDILDTANKVNQELYTTSSYKGLADAIAEATARLENGTAEEIDASVKAL
                     DNALKALVVRAKADEVKEYINSITLVDLSKYTDSSAKIYQEAYNVLKAMIT"
BASE COUNT         2381 a         1357 c          915 g         2574 t
ORIGIN      
        1 agtgtcacct gcttcattac gataagtttt tgcagctgtt tgtaattttg tgaactcgtt
       61 aattaaatca gtaaataaat catcactgat tgtattacca ctatttaaag cagttttaaa
      121 ttgattcaaa cgatctttta attctactga ctcttgtaat ggcgttacac gactatccat
      181 attttgattt atcatatgtt ttgatagttc ttttaaggca tccgaagcag ctgttacttc
      241 tgcgctatta tgatcaacat atttgaatga atcttcccaa atttcatctg cttcttcact
      301 agtgtccaag cctgatctgc ttcattggct gattgccaaa tattccatga ataacatgca
      361 tttccaaaaa ttgccacttt acttggttca gattgttgca ttggatttag aacaatacct
      421 tggatcttat ttggatcaac ccccggatgt aagaatgtac tatatccacc cataatcaag
      481 tgctttttag aattatcagt acaaggccaa ttgatccaca tatatggtcc acgacctaca
      541 ttattagtaa aagttgttgt aaaattattt gaaacttcac cccaaacacg tccaccagtc
      601 ataacaattt gaacgttttc tgggaaattc gtatagtaac cttggccatt acccatatat
      661 tcttgagtac aaaatggtaa tgttaatttt aaatctggat atgttttttg catttccttg
      721 atccatgcag tcatatcttt caaagtttta atgtagttat ctccaccaac atttccagca
      781 tcatcagcaa gaattgcaat ttgacgaaca cctgcttcaa ttacttgagc aaatttagcc
      841 tgcattactt ttaaatcagc ttgataattt tcttcactgt tatatctaat tgcattattc
      901 atatacggat gcaatgcaaa aacaaaacga cattttgatt cattaccagc ttgtgctaac
      961 ggttttattt ttgtttcaat ttcttcatca ctgtataatt cacgccattt agaattatgt
     1021 ttagggtcat cttttggagc ataaaaatat gaatttaatt tataatatcc tccccatttc
     1081 atcaattcag agcgatcttc tgttgaccaa gggtttccat aatatccttc aatgaatcct
     1141 cgactagcta catcagcgta atcttccatt acaaagttac ggattgaata actatccatc
     1201 tggttaaaga tatgatataa actagtgatt ccataaaatg cagcatcagt atctttacct
     1261 aatacagtaa ttgtcccatt atctgaggct aataaatatg aatctaattt atcatataat
     1321 ccacttgttt ttaatgtgta atttttagat acatgtttat caacgtatcc atctgatcct
     1381 ttaataccta ctaagatatt agtttgatct tctttaattt catnnnnnnn nnncatttga
     1441 gatagtagca tttatcttct tgattgccaa tacctcattc attctagctt tagtatattc
     1501 atcaatacca tcttcatata caatattaat attttgatga ataacgtaat caccactttg
     1561 atattccatc aaatgtggtt taggataaat ttcataatct gactcagcag cactaacttg
     1621 tgttggccat gcactaaaac aaccgaaaac cattagtact gccaaaaaca tagacatcag
     1681 tttatgagta ttttttttaa tttttctcat tatctccttt tcctttcccg tatagttaat
     1741 atcgagtccc attctcaaaa attatcaata ttagttatac attatataaa taaatcaata
     1801 cagattttaa acaaaaataa agaccaaata taattatgct aaaaaacata atacatattt
     1861 ggtcttgcct gcttagagta acaatccgta tttacaagta aatattagca aaatatttag
     1921 taaacgtcaa gcgcttccat tataattttg cgttcttttt tatatgtata cataaataag
     1981 tttgctcgtc tggtgataat tcataatcat aacgatcacg aattaattta acaatccgat
     2041 taatacacaa agacaatcgt aaatcttctt tcaacatttc ccggatatcc gctgtagtat
     2101 cttgcaattc atcaatttca tccctaaaaa ttctttccgc taaatacttt aaatgagttg
     2161 aaattcgcgc ataacctaga gaatcctcct ctaagtcaac tttcattgat aatttaatca
     2221 catttagtac ttcttttgtc aaagtaacaa ttttcgttgc attatttgcc ttattatcga
     2281 gtgaaaagtt tactaaatgt aaagcaatat aacctgcttc gtcgtcatct aaccgtatcc
     2341 ccgttttttc ttctatataa tcaagtgccc ataaaccaac tttatattct tttttatata
     2401 atacttttgt ttcactcaaa acaacattag gaagataaat ctcttccttt tttcttttta
     2461 ctgcaaatga aatatgatca cttattgcta aaaatatttc accactaaaa tctgtatcta
     2521 attccttatt agcttgatta acaattttat tagttatttc aaaatatata gcaggtactg
     2581 tttcaatcga ttgctcaaat cgtttctgct gatcatcttt aaaaaaatat gtcttttcaa
     2641 tcctgctttc gtcaacttca tcaccaattc tcttttgaaa accgatcccc gaaccagtta
     2701 aaattatatc acctaactca tctgatattg ctgcaacact attattatta aaaaccttga
     2761 gtatcttcaa tgttattgcc tccatccatt tacattaata tgtcattcat ggcatcgaca
     2821 ataaattgta cttcggtgcc tatgataatt tgtaaattat tttttccaat ttttataatt
     2881 cctgttgctc cagtagctaa tatagcttgt tcatcaacta aatcaggatt acctaattcc
     2941 acacgtaatc gtgtaataca gctatctgca aactgcacat tatttcgccc ccccaaagct
     3001 tctatcaaaa caattgcaat ctcatcatag tcttgatttt ctaatgtaat attgtattcc
     3061 tcttcctcaa tttcaattat atcttcatca gtctcccgac caggtgtcat taaattataa
     3121 cgaactatca tgaatctaaa aatgcaataa tatataaaag cacaaatcaa tccaagtgga
     3181 atcagcataa atatatcgtt agagaacggg gctttaatac tcaaaatata atcaatcaat
     3241 cctgccgaaa agccaaagcc cgcaagccat ttaaataccg cagccacaaa tagcattatt
     3301 cctgttaata gagcatgaat aaaatataat actggtgcaa caaacataaa agagaactcc
     3361 aatggttctg taaccccagt aacaaatgaa gcaaaagcag cagacaataa aatcgttcca
     3421 acttgttttt ttcgttcagg tctagcactt tggtaaatag cgattgcagc tgctggtaat
     3481 ccaaacatca ttactggaaa aaaaccagcc tgatacatac cagttactcc tttaatgcct
     3541 ccagacactg ttccccaaaa tttgccaata tcattaattc ctgccacatc aaaccaaaag
     3601 acagaattga gtgcatgatg taatccagta ggaattaata atcgattaaa aaatccatat
     3661 aatccagctc caactggtcc taatttcgaa atcatttctc caaatgatac aaaacattca
     3721 taaaaaaacg gccaaacaat tagtaataat aaagaaataa ctagcattga agcagtcgat
     3781 attattggga caaaacgctt tccaccaaaa aaagataacg catcaggtaa ctttactttc
     3841 gaaaactttt tataattata tgccactaca agacctgaaa ttattccaac aaatgcattt
     3901 gcagaatttt caaaagcagc agaaacttct gcttcgggaa ttccttttat caatgataca
     3961 gccggtggtg aaagcagcct agaaacaatc atataagaaa caaccgcact tagagctacc
     4021 gttaactctc gttcctgaga cattccaata gcaattccta cagcaaataa aattggtaaa
     4081 ttatcaataa tcgctccccc agattcaatc aaaaaagcag caatagcatt attacttccc
     4141 catcctgttg ggtcgatcca ataaccaatt ccctttaata atgcagcaat tggcatcact
     4201 gatacaggca acatcaatga ttttcccaat ctttgcatgt atttcatcat tcccatcccc
     4261 ccaatcaata taggatttaa ctgtaccttt ccattcaata tatttatgtt tctaaacnnn
     4321 nnnnnnnnnn nnnnnnnnnn attgattatt agtaaattaa tgtgtttttt tgtccgaacg
     4381 aaaatgtact atttttcgtg cagaataata tatccaaacc tattgtatca ctaaagcgct
     4441 tactttgtgt taaaaaaaat acaacgaact aagttcgcac tatgtaagaa attatacctt
     4501 ttattatcaa atcatgtcaa tagatatatc tgtggctata tacattaaat cataactaca
     4561 tattatcatt ttataaaata attggtataa tataagaata tttaaagata ttcttgacat
     4621 ataattattt agtcgatatt ataaatataa cgatgttact attaaattag gcattagtga
     4681 ttattttttg atatcactaa tgcctttttc tatatttaaa aggagataat gaatgaattt
     4741 ttttactaga ctattaaaaa ataaaaaaaa tacagtatgt gctcctgcct gtggcaaagt
     4801 tattcgtctg gaaaatatcc ccgacaaagc atttgctaac aaaatggtcg gtgatggtct
     4861 tgcaattgaa attaccgata ataaacttgt tgcaccatgt gagggaataa ttagtgttat
     4921 tgctaatact aaacacgcat ttgttatgac ccttcctaat ggtcttcatt tactagtaca
     4981 tattggtatc aatagagcaa agccagatgc taataatttt cagtatcatg taaaagccgg
     5041 tgactatgtt tctttaggag cagatattgt tacactaagc gataatttat taaaagtcct
     5101 aaataataag gttattaccc ctattattgt ttgcaactat gaatctcacc ctattaaaac
     5161 atttactact gcttcatctg ttgaaactgg taaaaccatt tttacttata aatgattgag
     5221 tctaaattaa atgaagcccc ttatttggat aaccaaataa ggggcctcat ttcaaaaatt
     5281 aggctcgttt tttatttaca gtataaaaag cgaaatttaa atttcgcttt ttaacatcta
     5341 ttaaattatt cctctttttt tcttaacagc cagtatatac caacaatagc aacaacgcct
     5401 aatcctgtta cataggcaaa aatatttaca tcatcacctg ttttcgcaga atcactagca
     5461 ggaagttcgt ttggagtcgg tgttggtatt ggggctactg ttgctttttc ttctaattta
     5521 gcaaccgctg tttcaaaatt gtttttagcc tcaataaatt ctttagcaga aatatcactt
     5581 aagttatcat agcttttaaa acattatatg cctcttgata tattttagca gaactgtcag
     5641 tgtatttgct taaatccact aatgtaatac tgttaatata ttcttttact tcatcagctt
     5701 tagcacgaac tacaagagct ttaagtgcat tatcaagtgc tttgacacta gcatcaattt
     5761 cttcagcagt tccattttct aatcttgcag ttgcttcagc aattgcatca gctaatcctt
     5821 tgtaactgct agttgtatat aattcttgat taactttatt agctgtatct aaaatatctt
     5881 ttagatcacc aacaactact aatttttctt tagcagcagc taattcacta atagctttcg
     5941 caaattcatc agggtgtaca ggagtgcttt gattatctat aatcaactgt tcaacttttg
     6001 ccttagcttc tagatacatt ttccatgagt tgattgtgta aagtttttct ggatttgtaa
     6061 ccgaagctac aaaatcattt tctccttcaa ttgtaataat tgcttcttct tgatttgaag
     6121 gattaaatac taaattagta tttgctagat cataattagc aattaattta tcaacatctg
     6181 caactgaagt attatcacca ttagcaattg acttattaat tgcctcaata gctttactat
     6241 atgctaacca tgaagccgca gtgtaattat tttgatcagt atgtgcatta gcgataatta
     6301 tttccaattt acttaaatca ccttttaata ctttattttc aattgcctta ttaattgctc
     6361 taacggcatt atcaacagtt gtttgtgaag catactcact ttcaagtact tgcttacctg
     6421 cattataagc atctccatat gttgcagctg ctgcagctgt ccatgaagat aaatcttcct
     6481 tgttatcaat taaagcttta agcgcatcaa cattaagtgt agtgacctca gatttgtatg
     6541 ttgataattc tgtaatatta ggaatcacat caccccactc aaccttaata tctaataaga
     6601 ttgtattttc aggtaaaaca aattcattaa ttgcttgtga taatgttcct aaagttaccc
     6661 attgttctgc acttgcattt gtaaataatc ttgctttaac aactgcatta gaaattacag
     6721 cactattttg aataatttta attgtgttta cttggttatt agatgataaa ctatagttca
     6781 cataaccatt agcatcttta ggaataaaca ttgttgaaat atccccatca accatataac
     6841 taaataatcc atctcttgtt tcttgactac tagatgtaaa ggttggattg ttttgtgaag
     6901 gtataaattc cccattattt atttctaatt cttggaatct aacccattta tcagctccaa
     6961 ctttagttct agtaatttca aattttaaat aacgagcttg cagatttaaa ccatctactt
     7021 tttttgcatt atatgaaatt tcatgacttg gtaaaacaaa actaatttca tcagtatttt
     7081 cagcttcacc tgggttattt tcattttgat tacctaaaag catgatttct gtccaagttt
     7141 caccatcagt agaaactgag aactttccat gacgtggata atcccattcg ctatcagtac
     7201 atatagcttt aaatgaattt aaagttattt cttgacctaa atcatatgtg aaatacttgc
//
LOCUS       DAOMTA010000038         7253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_132_length_7253_cov_2.405251, whole genome shotgun sequence.
ACCESSION   DAOMTA010000038 DAOMTA010000000
VERSION     DAOMTA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_7253_cov_2.405251"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLK68_01885"
     CDS             complement(<1..338)
                     /locus_tag="PLK68_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89189.1"
                     /translation="MFMCLAYFLSIVNNSNNKDLNYYIAKYIIENINHIGSITLQDVA
                     DGCFVSVPTVKQFLKKFGYSNYSIFKERLESELDVRKDQIYRGYKIFNKKRLAVAVSH
                     LVDYPLEFND"
     gene            complement(394..1287)
                     /locus_tag="PLK68_01890"
     CDS             complement(394..1287)
                     /locus_tag="PLK68_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89190.1"
                     /translation="MKYLCLDFGGTGVKYAIIDENFQLYDVNKENKIFQSHDEMITWV
                     VDLFKTIHMKLSGIAISYCGEMNPFTGLIKNGGSYRFNDNKNIKQILWSKCHVPVSIE
                     NDGNCAALAELYRGSLKETSNGAVLVLGTGVGCAIIINGELYRGCNYFAGAATFSLID
                     SKHDFDWQNTFGLIGGVGYLTRNYEKRFALERNSIDGLAFFNKANHNDIASLEILRDY
                     TKNLARYIFNMQMLLDLENISIGGGISTQPLLLTMLKKEVSDLFDCIPVPVTPPEIKV
                     CHYYNDANLIGAFCWYKKANF"
     gene            complement(1412..2611)
                     /locus_tag="PLK68_01895"
     CDS             complement(1412..2611)
                     /locus_tag="PLK68_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HRM89191.1"
                     /translation="MGFTIEDALVQTQEQYHLKLLAGREGCSNAMSWVHMIEDTTIIQ
                     QLWGKELAVTTGLGFQSHDSLFDFIKCLVKYHSVGLVINTGKYIFEIPQDIIDYCNEQ
                     DFPLLTTPWEVHMADLIKDFSMRCLYSQREDNQISKLFQNVFTTPQVVEEVRQQLLGA
                     FDVDGFFQVVLIGIEDSDQFDAIERRRVSFQLELCFEKIQSPYTFFWFDGYFVLIVNN
                     LDPDSLEKINDKMYKRTKKRMPSRFIHLGIGTPMTDFRQVILSYKRARAAVAMAEQFK
                     YPMILFEEMGVYQILFSIEDKQILSGMYQHLLKPLIDYDQKHHSELEKTLFYYLIHDG
                     SQVAMAKNLYMHRNTINYRMTKVKELLNCQLDTFEEKMPYMLALYIKKVIQEDINKDK
                     DLFAAIQ"
     gene            2763..2891
                     /locus_tag="PLK68_01900"
     CDS             2763..2891
                     /locus_tag="PLK68_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89192.1"
                     /translation="MNISYGYYVLTDNFDTCPTWVRDEGYFNCENFSKFFKFFKKK"
     gene            complement(2957..4897)
                     /locus_tag="PLK68_01905"
     CDS             complement(2957..4897)
                     /locus_tag="PLK68_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89193.1"
                     /translation="MALKKLFTPYQIGTVKIPNRLVVPAMVTNYCTIEGKITERYMKY
                     IEEKAKGGWGLIITEDYAVQQYGKGYQRIPGLYKDELIEGNKQLTTMVHKYESKIFCQ
                     MYHPGRQTTRIANEDHMPVAPSAIECPLCQEQPRDITVAEINQLVKDFGSAAKRVKEA
                     GFDGIELHCAHGYLLAEFLSPFVNKRVDNYGGCLQNRVRIVAEIYLEMRKQVGDDFPI
                     IVRLSGNEYVHGGRTEAETYELCTIFEELGFDGIHISNGSYASPGNRAVIAPMFTEHA
                     LNMEISAQVKKLVDLPVIVTNRINDPQMADTLINMDKADFIGMGRGSLTDPDLPNKAK
                     AEKYGNIKMCIGCLQGCEMPLFFNQEVTCLVNPRVGREYENSMDIVEKAKKVMIVGGG
                     PAGLQAAETAAMIGHNVTVYEAQEEVGGQFRSAAYPIGKGELTTLISVFKKNLEDLHV
                     KVHLNTLVTKEMIEQENPDAIILATGARPLVPAVKGIDKENVVNAEDVLLGKIATKVE
                     HIVVCGGGEVGCETATFIAQTHRHVTVLEMKPAVLTDMDPINMSCLLPIMNESGVTAR
                     ANCTVTEILDDGVAFINEQGEKEVISADLVVLAFGYKAYNPLEKIANELCEDVQVVGG
                     AIKAGNALPAVKEGYEAALNIK"
     gene            complement(5038..5856)
                     /locus_tag="PLK68_01910"
     CDS             complement(5038..5856)
                     /locus_tag="PLK68_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM89194.1"
                     /translation="MNSQYKIGDISRLFNLSSEMIRYYEKMGIIDPIRDEKNGYRYYS
                     IFDIYILLECLQYQNLGMKIKEIPNFINFNYREKLQNQLHIFQRRLNKEIDYKVMLKK
                     RVTELAMQLEVADLNLSNYWVKKINKKYAFDFVDGIGDDYDKVNTSEDNIRFLLNDKY
                     INFIDSYVCFNKDKDEWYFAIDEEYFNGLSISYKKKYTIIPAHYCLCTVIEMGPLGQF
                     SHECYEAAVMYAQDKGYHVQLPIRGIIRGRGYEGDRFKRYLEIQIPIIKNSQIA"
     gene            complement(6890..>7253)
                     /locus_tag="PLK68_01915"
     CDS             complement(6890..>7253)
                     /locus_tag="PLK68_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRM89195.1"
                     /translation="FGLAACQTAYESCSSWLEDLKQYLFENTRFVDEYLKEHIPLIKL
                     IIPEGTYLLWLDFRRLQISDKELNDLLIEHARLWLDSGTMFGKEGKGYQRINIACPRA
                     YLRQALNQLKNALEEIEY"
BASE COUNT         2484 a         1341 c          797 g         2631 t
ORIGIN      
        1 ttatcattga attctaatgg ataatcaact aaatgtgaaa ccgctacagc taatcgtttt
       61 ttgttaaaaa tcttataacc tcgatatatc tgatctttac gaacatccaa ttcactttct
      121 aatctttctt taaaaatcga ataattagaa taaccaaatt tttttaaaaa ctgttttact
      181 gttggaactg aaacaaaaca gccatcagcc acatcttgta aagtaatact tcctatatga
      241 ttaatatttt ctataatata tttggcaata taataattta aatctttatt attagagtta
      301 ttaacaattg ataaaaaata agctaagcac ataaacatat tatcttcctc cactcattat
      361 taccattata taatattaat taaatttatt catttaaaaa ttagctttct tgtaccagca
      421 aaaagctcca atcaaatttg catcattata ataatgacac actttgatct ctggtggagt
      481 taccggaact gggatgcaat caaataaatc actcacttct ttctttaaca ttgttaataa
      541 taacggctga gtactaatcc ctccaccaat actaatattc tctaaatcta ataacatttg
      601 catattaaaa atatatcttg caagattttt agtataatcc cgtagtattt ctaaactagc
      661 aatatcatta tgattagcct tgttaaaaaa ggctaatcca tcgatcgaat ttcgctctaa
      721 tgcaaatcgt ttttcataat ttctcgttaa ataacctaca ccgccaatca atccaaatgt
      781 attttgccaa tcaaagtcat gtttagaatc aattaaagaa aacgtagctg ctccagcaaa
      841 ataattacac ccacgatata attccccgtt aataataatt gcgcatccaa ctccagtacc
      901 caatactagt actgccccat tactcgtttc ttttaatgag cctctataca attctgctaa
      961 tgccgcacaa ttaccatcat tttcaatcga aactggtacg tggcatttac tccaaaggat
     1021 ttgtttaata tttttattat cattaaatct ataactacca ccatttttta ttaaacctgt
     1081 aaagggattc atttccccac aataactaat cgcaattccg gataatttca tgtgtatagt
     1141 tttaaataaa tccactaccc aggtaatcat ttcatcatga ctttgaaata ttttattttc
     1201 tttatttaca tcatataact gaaagttttc atcaataatt gcatatttaa ctccggtgcc
     1261 accaaaatct aaacataaat atttcattat ttaatccctc atttcttaac actttaaagt
     1321 tatcatagtt aaaaaaaata taaatactat ataatttttg gttgtgtcaa aaaatgaaac
     1381 atagaaatct aatatgaact gttagctatg attattgtat tgcagcaaat aaatctttat
     1441 ctttgttaat atcttcttga atgacttttt tgatatataa agctaacata tatggcattt
     1501 tttcttcaaa tgtatctaac tggcaattta ataattcctt gactttagtc attctataat
     1561 taatcgtatt tcggtgcata tataaatttt tggccatcgc tacttgactg ccatcatgaa
     1621 tcaggtaata gaacaatgtc ttttctaatt cactatgatg tttttgatca taatctatta
     1681 aaggctttaa taaatgctga tacattcctg ataaaatttg tttatcctcg attgaaaata
     1741 agatttgata aactcccatc tcttcaaaaa gaatcatcgg atatttaaat tgttctgcca
     1801 ttgcaactgc agctcgagct cgtttataac ttaagattac ttgtctaaaa tctgtcattg
     1861 gcgttccaat acctaagtga ataaatcttg aaggcattcg cttctttgtt cttttataca
     1921 ttttatcatt aattttctct aatgaatccg gatctaaatt atttacaatt aatacaaaat
     1981 atccatcaaa ccaaaaaaat gtatatgggc tttgtatttt ttcaaaacat aattccaatt
     2041 gaaaagaaac tctacgtcgt tcaattgcat caaactgatc agaatcttca attccaatta
     2101 aaacaacttg aaaaaaacca tccacatcaa atgcacctaa caattgctgc cgaacctcct
     2161 caacaacttg aggagttgta aaaacattct gaaataattt tgaaatctga ttgtcttctc
     2221 gttgtgaata taaacagcgc atactaaaat ccttaattaa atctgccatg tgtacttccc
     2281 agggtgtcgt taacagcgga aaatcctgct cattacaata gtcaattata tcttgaggaa
     2341 tttcaaatat atatttacct gtattgataa ctaatccaac actatggtat ttaaccaaac
     2401 acttgataaa atcaaataac gaatcgtgac tttgaaatcc taaacctgtt gtaactgcca
     2461 attcttttcc ccataattgt tgaataattg tcgtatcctc aatcatgtga acccaactca
     2521 tcgcatttga acagccctca cgaccagcta acaattttaa atgatattgc tcttgagttt
     2581 gtactaacgc atcttcaatc gtaaatccca ttttactcac ttcctttata tttattttac
     2641 cattatttgt gcatgatgca caaataaatc ttattttttt attatttctg tctctataat
     2701 tattaaaata ttaatatata atataattgt attaaaaata gaacatctag gagggacata
     2761 acatgaacat ttcttatgga tattacgtac taactgacaa ctttgatact tgcccaactt
     2821 gggttcgtga tgaaggatat ttcaattgtg aaaacttttc aaaattcttt aagtttttta
     2881 aaaagaaata aataataatg agcattcata acgaatgctc tttttctttt aagatttaaa
     2941 ttatgctatt agataattat tttatattta aagcagcttc ataaccttct ttaactgctg
     3001 gtaaagcatt acctgcctta attgcgccac ctactacttg aacatcttca cataattcat
     3061 ttgcaatttt ttctaacgga ttataggctt tatatccaaa tgctaagaca actaagtcag
     3121 cagaaataac ttctttttct ccctgttcat taataaatgc cactccatca tctaaaattt
     3181 ctgtcacagt acaattagct ctggcagtta ctcctgattc attcataatt ggtaatagac
     3241 agctcatatt aatgggatcc atatcagtca aaacagctgg tttcatttct agaactgtta
     3301 catgacgatg cgtttgagca ataaatgtgg cagtttcaca accaacttcg ccccctccgc
     3361 aaacaacgat atgctcaact tttgtagcga tttttcctaa taacacatct tcagcattga
     3421 ctacattttc tttatcgata cctttgactg ccggcaccag cggtcttgct ccagtagcca
     3481 aaataatagc atccgggttt tcttgttcaa tcatctcttt tgttactaaa gtatttaaat
     3541 gtacttttac atgcagatcc tctaaattct ttttaaatac agatattaat gttgttaatt
     3601 ctcctttacc aattggataa gctgcagatc taaattgacc gccaacttct tcctgagcct
     3661 cataaacagt tacattgtga cctatcattg ccgcagtttc agccgcctgc agaccagcag
     3721 gtccccctcc aacaatcatt actttttttg ctttttctac aatgtccatt gaattttcat
     3781 attcacgtcc tacccgtgga ttaactaagc aagtgacttc ttgattaaaa aataatggca
     3841 tttcacatcc ttgtagacac ccgatacaca ttttaatatt cccatatttt tctgcctttg
     3901 ctttattagg taaatctgga tcagtcaatg aaccacgccc cattccaata aaatcagctt
     3961 tatccatgtt aattaatgta tctgccattt gcggatcgtt aattcgatta gttacaatta
     4021 ctggtaaatc aaccaatttc tttacttgtg ctgatatctc catattcaat gcatgttccg
     4081 taaacattgg agcaattact gctctatttc caggtgaagc ataagagcca ttagaaatat
     4141 gaataccatc aaaacctaat tcttcaaaaa tcgtacataa ttcataagtt tctgcttcag
     4201 ttcttccacc atgaacatat tcattaccag aaagacggac gataattgga aaatcatcac
     4261 caacttgttt tctcatttct aaataaattt cagcaactat tcgtactcta ttttgtagac
     4321 aaccaccata gttatcaact cgcttattga caaatggtga taaaaattcc gctaataaat
     4381 aaccatgggc acagtgtagc tcaattccat caaaaccagc ttctttaacg cgtttagcag
     4441 cactaccaaa atcttttact aattgattga tttcagcaac tgtgatatct cttggttgtt
     4501 cttgacataa gggacattca attgctgaag gagcaactgg catatgatct tcattagcaa
     4561 ttctagtagt ttgtctacct ggatgataca tttgacaaaa gatttttgat tcatacttat
     4621 gaaccatcgt tgttaattgt ttatttcctt caatcaattc atctttatat aaaccgggaa
     4681 ttctttgata tcctttacca tattgttgaa ctgcataatc ctcagtaata atcaaacccc
     4741 agccgccttt agccttttct tcaatatatt tcatataacg ctcagtaatt ttaccttcaa
     4801 tcgtacaata atttgtcacc attgccggaa ctactaaacg attaggaatt tttacagttc
     4861 ctatttgata aggggtaaat aattttttta aagccatttt aatatcctcc aatcctttta
     4921 taactttatt ttacagtatc aaatgatttg aaggtcaact ctaatttatt atatgtgata
     4981 ttattcacat attctttttt aatcacatat attttactaa aacaacataa aaaactctca
     5041 agctatttga gagtttttta taatcggaat ttgtatttct aaataccgtt taaaacgatc
     5101 accttcatat ccacgccccc taataatccc ccgtattggt aactgtacat gataaccttt
     5161 atcttgagca tacattactg ctgcttcata acactcatga ctaaactgtc ctaacggtcc
     5221 catttctatt accgtgcata aacaataatg agccggaata attgtatact tctttttata
     5281 agaaatactt aatccattaa aatattcctc atcaatagca aaataccatt catctttatc
     5341 tttattaaaa caaacataac tatcaataaa attaatatat ttatcattta ataaaaatcg
     5401 aatattatct tcactagtat taactttatc ataatcatca ccaattccat ctacaaaatc
     5461 aaaagcatat tttttattaa ttttctttac ccaataattt gataaattta aatcagctac
     5521 ctccagttgc attgccaact ctgtgactcg ttttttcaac atgactttat aatcaatctc
     5581 tttatttagt cgcctttgga aaatatgtaa ctgattttga agcttttctc tataattaaa
     5641 attaataaaa ttaggaattt cttttatttt cattcctaaa ttctgatact gtaaacattc
     5701 taataaaata tatatgtcaa aaatactata atagcgatac ccattctttt catctcttat
     5761 aggatcaata attcccattt tttcataata cctaatcatt tcacttgata aattaaacaa
     5821 tcgtgaaata tcccctattt tatactggct attcataata tcagctcctt tacaaataat
     5881 tttatcactt aatcttccta tttaaaaaat aattttatta ttagttttta taaatcaact
     5941 attggtagat ttataaatta attaaaactc gttataatca aaatataaag gagaaatcaa
     6001 tagatggatt gtattaaaat tggaaaattt attgcgacat gctgtaaaga aaaaacttaa
     6061 cccagattga aaaactaaat gtaacaaata aagctatatc aaaatggtcg aaattttatc
     6121 gatgcttcat tttatcacga ttttcgcaat atcttaggca ttactattaa cgaattatta
     6181 gctggtgaac atctcaatac tcaagataat caatattgct atgaaatgaa tcttcacaat
     6241 cgtattaaca gttacaatat cataaatcga aaaaataaaa taattatttt catgattttt
     6301 agtacattga ttttttagtc attacgtatt tacaaataaa aactatatgg atttttatca
     6361 tggattcaat acatctaaaa atcattattt agaaaagcta attcttgatc tatttcattc
     6421 tttatcagtc cctggcaaca cacatccaaa ctttgatgaa ctttattatt ggaaaatatt
     6481 tgaacagtct gatagtagta aaatgtacta atactaagca atattatttt attgaaaata
     6541 tattctaaac aaaataaaag gtatcacgaa ttatatggga gagggtccgt gataccttat
     6601 atgactatta tagcatattt aaaaaaataa gcatttaatc tgattaaact ttaattagtt
     6661 tctccaaaag aaatctagat ttcaagtatt ttagtctaaa tatattatac ttttaatctc
     6721 tattacaaag atttagaaat ctttatcaaa tcaactaaaa caaatctatt ctacatttca
     6781 gttagagcat tgttttttat aataaatcat ttttacaagt catataccta actaaccact
     6841 ataccaataa ttactttaat aatatttcaa gaaattgata cttaaaatat taatattcaa
     6901 tttcttcaag tgcatttttc aattgattca aagcttgtct taaatatgct cttgggcaag
     6961 caatgttaat tctttgatac ccttttcctt cttttccaaa catcgttcca ctatctagcc
     7021 ataatcgagc atgttcaatc aataaatcat ttaattcttt atctgaaatt tgtaatcttc
     7081 taaaatcaag ccaaagcaaa taagttccct ccgggataat caactttatc aatggtatgt
     7141 gttctttcaa atattcatct acaaaccgtg tattttcaaa taaatattgc tttaaatctt
     7201 caagccagct tgaacacgat tcataagctg tctggcaagc tgctaatcca aac
//
LOCUS       DAOMTA010000039         7246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_133_length_7246_cov_2.419135, whole genome shotgun sequence.
ACCESSION   DAOMTA010000039 DAOMTA010000000
VERSION     DAOMTA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133_length_7246_cov_2.419135"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..469)
                     /locus_tag="PLK68_01920"
     CDS             complement(200..469)
                     /locus_tag="PLK68_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89196.1"
                     /translation="MRPDKAQEIKSDTSVANVIANQIPDKDCFKEIKDIAKRAWWLGC
                     DSTHYETRYKDHDINDLKECIDITVATIVFYLKREHYLKMIQKNP"
     gene            936..1397
                     /locus_tag="PLK68_01925"
     CDS             936..1397
                     /locus_tag="PLK68_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89197.1"
                     /translation="MQQLKKFICLLLFLFIMTACQEKIAYDKEWAANFAQQQVKAMDG
                     YRLEGVYFYQGNHIRVNDQKVGKAVVVKAVVRYYDVFKDPNKYELVSVFDESNIDLAG
                     QGYIYGEDGDRLLGVDQIDVYDSNQPIKYLRAMDDKDISEVNRKLNTVQIK"
     gene            complement(1481..2293)
                     /locus_tag="PLK68_01930"
     CDS             complement(1481..2293)
                     /locus_tag="PLK68_01930"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRM89198.1"
                     /translation="MYIKINEATNYIKTQYHGKIDLAIILGSGLGPLADEIENPIELD
                     YRDIPHFPISNLIGHAGKLIIGTLENKTVIAMKGRFHYYEGNDMDIVTLPIRVFKRLG
                     IDNLILTNACGGIREDLNPGQIMLIKDHIGLFAPSPLRGPNLDEFGPRFKDMSEVYNR
                     KLGKLAHQVADDNNISLKDGVYAFFKGPMYETPAEILAYRALGADAVGMSTVPEAIVA
                     RHCDMKTLGISLITNKAAGLSNQELNHQEVVEAANKAEKDLVTLVKGIIKNW"
     gene            2372..2524
                     /locus_tag="PLK68_01935"
     CDS             2372..2524
                     /locus_tag="PLK68_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HRM89199.1"
                     /translation="MERKLKTRHLYRHFKDKLYYVMNIGLDSETLEEVVIYQAMYDDK
                     KHLFVL"
     gene            2610..3842
                     /locus_tag="PLK68_01940"
     CDS             2610..3842
                     /locus_tag="PLK68_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM89200.1"
                     /translation="MNQAAYRLEQTKLTDSKVFRDAVHNYIHVDQPLILDLINSHEMQ
                     RLRRIKQLGGTHQVYQSAEHSRFCHSLGVYFIARKMIFNSAIGAYLNDYDKLTVMCAA
                     LLHDIGHGPFSHCFEDAFDLNHEAYTIKIINGKTEVHDLLEKFDHGFSHRVSSVIEKT
                     HPNKILVQMVSSQLDADRMDYLLRDSYFSGTTYGQFDLSRILRVMAVCDGKIVFKNSG
                     VQAIENYILARYHMYWQVYYHPTARSYEQVLISIFRRMKDLYAAGYDFGDIRYLKPFL
                     DGHVDENDYTKLDEGIVFYYFTVLKEGNDEILKDLCTRFLDRRLFIYHDLLDQHEKQL
                     AESFYEKKGYDPRYYVVSDDQSQVPYRYYGNTEELSEIEILIDEELRFLPEVSEIVGA
                     IVNSKKNKNDHKIFYPEV"
     gene            complement(3852..5729)
                     /locus_tag="PLK68_01945"
     CDS             complement(3852..5729)
                     /locus_tag="PLK68_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRM89201.1"
                     /translation="MKKLVKIFVTILASLAGILLSLYVIAYCMGEPDLNKNRYLKLYD
                     DNQEIYYQSINDYTGHYVTLNNVSNYFKESIVAIEDQRFYDHRGFDPIGIMRALKVNF
                     TNQKKSQGASTITQQYARLLYLTNEKSWSRKIKEAFLTMQLETRLSKDEILEGYINNV
                     YFGHGIYGIENAAQYFYGKSAKELDLNESSMLAGVVNGPTYYSPLNDAKAARKRQSIV
                     LNALIDCKEITVTQKQQVLDSDLNLAETHKVDDNMANNYYKDTVIDELEELGYYNNTY
                     LNQGLNVYTSFNQDYQKVVEKSASEYTKDSKVETSSIVVEPYTSKILAIIGGKDYASS
                     QFNRATQANRQIGSTIKPLLYYLALENGFTPATTFLCEPTTFKLDDNSTYSPSNFNDK
                     YAYKDVTLAQAIAVSDNIFAVKTHLFLGTDNLASLIKKFGIKDVKANASLALGTLNTN
                     IYNLANMYNCLASEGKYNHLYTIEKITNDAGKVLYQHEQEDKQLLEQDSCLELSQLLT
                     STFNSTFSTYLQATMASYQLDNINACKTGSTDVDNLAVAYNPQVLVASWVGYDDNRKM
                     ETADDKTVAKKTVIDVLNYTNENKDVTWYQPTEDLQQIAINPLTGDFDENGAVYWFKK
                     D"
     gene            5785..6165
                     /locus_tag="PLK68_01950"
     CDS             5785..6165
                     /locus_tag="PLK68_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HRM89202.1"
                     /translation="MKKIIKVNYRSIFKYEEHQETVKYDGSGHLEIGDDKIVVSYQDE
                     NKIKIELSENEVKLHNGASVLHLVRDRDILNQYETPYGAIALKTRLISYDNGDNVKIK
                     YELYDGTNLISQVYVMLNYLILEN"
     gene            6167..>7246
                     /locus_tag="PLK68_01955"
     CDS             6167..>7246
                     /locus_tag="PLK68_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRM89203.1"
                     /translation="MKILKFLSNRIVVSIILICVQILWLALFMRYIFTDATLLKGVFI
                     FLSVIVTLYIINNDDDDPSYKIIWLIPILSFPVFGGLLYVVFGNKKPAKKLQEAFANQ
                     EEAIRPFIYPNKMVEQIEDLIAKGQANYLVQENFPIYNNSEIKYYPLGDDTYPDLLKE
                     LAKAKHFIFMEYFIVEEGEMFNTVLTILKQKVKEGVEVRFMYDDMGSLTMLPFRYYQK
                     LESYGIKCIAFNHFVPFISAVMNTRDHRKITVIDGNVGFSGGFNLADEYINAKVKYGH
                     WKDTGVMIKGEAVWNLTLMFLTTWNASLNTFEDYDKYHPRHYICPEISPNGYILPYGD
                     SPLDNKPVGKNVYLNMINQAQKYIYI"
BASE COUNT         2390 a         1013 c         1314 g         2529 t
ORIGIN      
        1 ggaaaaggta tacataattg acagttaaat aagtcaatta caatatacca ggagtaagtt
       61 atggaaaaga atcaaattag ttctgtaaaa gtagaagaag ttattgctat aagaactgtg
      121 acaggtgatg gagcagaaaa aagtccatat actgcagtaa tacagtattg gactaaatcg
      181 ggaaaaatga taggtcaact tagggatttt tctgaatcat tttcaaataa tgttcacgtt
      241 tcaaatagaa aacgatagtc gccacagtta tatctatgca ttcttttaaa tcattaatat
      301 catgatcctt atatcgtgtt tcgtaatgtg ttgaatcaca accaagccac catgctctct
      361 tagcgatatc tttaatttct ttaaagcaat ccttgtccgg aatttgatta gcgataacat
      421 ttgctacact tgtatcgctt ttaatttctt gtgctttgtc tggacgcaag aatgatgcaa
      481 agtcctttat taataattct agagatagtc ggtatccaga accacatatt ttatttaatc
      541 cgatattttc tgcaacagct tattgaccgt aagtttctac aaactttgga gaaatctcat
      601 aaataaattt atcaaaataa tcatctggaa aagaataggg gtagatatct acatcttgaa
      661 gggtaaggtc gtattctata ccatataacc cattgcgtgg attatattcg cctttatatt
      721 ttacaaagaa aatttcatta catgaaattt tttcatggtt tattaaaaat aatgagcttt
      781 acgattagtc cgttaatcgg ttaaatgact agtatttatt aggatttgta ttatttttat
      841 agattttgta aatatcatta gaacttagat aatataaaga ttatttaaaa taaaaaaata
      901 tttttatgat ataatagctt gtggaggaga ggataatgca acaattaaag aaatttattt
      961 gtttattact atttcttttt ataatgacag cctgtcaaga aaaaatagca tatgataagg
     1021 aatgggcggc aaattttgca cagcagcaag ttaaggctat ggatggatat cgtctggagg
     1081 gagtatattt ttatcaagga aatcatatta gggtaaatga tcaaaaggtt ggtaaggcag
     1141 ttgtcgtgaa agcagtagtt agatactatg atgtttttaa ggatccaaac aaatatgagc
     1201 ttgtttctgt ttttgatgaa tcaaacattg atttagcagg acaaggatat atttacggtg
     1261 aagatggaga taggttatta ggggtcgatc aaatagatgt ttatgattcc aatcaaccaa
     1321 ttaagtatct tcgggctatg gatgataaag acattagtga agttaatcga aaattaaata
     1381 ctgtgcaaat aaaataaaaa agcatcttta aattgttccc tatagagtaa acacgataaa
     1441 tgtgttttct atagggtcag tttagtatga tgcttttagt ttaccaattt tttataatgc
     1501 cttttactaa agtaactagg tctttttctg ctttattagc cgcttctaca acttcttggt
     1561 gatttaattc ttggtttgaa agtcctgcgg ctttattagt gatcaacgaa attcccagag
     1621 ttttcatatc acagtgacga gcaacaatgg cttctggtac agtggacatt cccactgcat
     1681 cagcacctaa ggctctatat gctaatattt ctgctggtgt ttcatacatt gggcctttaa
     1741 aaaaagcata gacaccatct tttaaactaa tattattgtc atcggcaact tgatgagcaa
     1801 gttttcctaa ttttcgatta tagacttcac tcatatcttt aaaacgaggg ccgaattcat
     1861 cgagattagg accacgtagt gggcttgggg caaataaacc aatatgatct ttaatcagca
     1921 taatttgtcc aggatttaaa tcttcacgga ttcctccgca agcattagtt aaaattaaat
     1981 tatcaattcc cagtctttta aatacacgaa ttggcagtgt aacaatatcc atgtcattgc
     2041 cttcataata atgaaaacgt cctttcatgg caattactgt tttattttct aatgtaccga
     2101 taattaactt gccggcatgt ccaatgagat tagaaatcgg gaaatggggg atatcgcgat
     2161 aatctaattc gataggattt tcaatttcat ctgcaagtgg gcctagacct gaaccgagaa
     2221 tgattgctaa atcaattttt ccatgatatt gggtcttaat atagtttgtt gcttcattga
     2281 ttttaatgta catatgtcat acctcctaga ctcaattata gcataaactt ttaagatgta
     2341 ttataatgaa aatagaaaaa gtggtgagaa cgtggaacga aaattaaaga ctcgacattt
     2401 atatcgacat ttcaaagaca aactatatta tgttatgaat attgggttag atagtgaaac
     2461 attggaggaa gttgttattt atcaagctat gtatgatgat aaaaaacatt tattcgtcct
     2521 ttagaaatgt ttttatcaaa agttgatata aagaagtacc caaatgtaga ataaacatat
     2581 cgttttgagg aatacgagga ggaagactga tgaatcaagc ggcatatcga ttagaacaga
     2641 cgaaattaac agatagtaag gtttttcgtg atgcagtaca taattatatt catgtagatc
     2701 aaccattgat attagattta atcaatagtc atgaaatgca gcgacttcgg agaatcaagc
     2761 aattaggcgg gacgcatcag gtctatcaaa gtgcggaaca ttcgcgtttt tgtcattctt
     2821 taggggttta ctttattgct cgaaaaatga tttttaatag tgcaatcgga gcctatttaa
     2881 atgattatga taagttaaca gtaatgtgtg cagctttact ccatgatatt ggtcatggtc
     2941 cattttcgca ttgttttgaa gatgcctttg atttaaacca cgaagcatat acaattaaaa
     3001 ttattaacgg aaagaccgaa gtacatgatc ttttagaaaa atttgatcat ggtttttcac
     3061 atagagtaag tagtgttatt gaaaaaacac atcctaataa aattttagta cagatggttt
     3121 cgagtcaact tgatgctgat cggatggatt atttacttag agattcttat tttagtggta
     3181 ctacttatgg gcaattcgat ttatcccgaa ttttacgagt gatggctgtt tgtgatggaa
     3241 agatcgtttt taagaattct ggtgtacaag caatcgagaa ttatatttta gccagatatc
     3301 atatgtattg gcaagtttat tatcacccca cagcccggag ttatgaacag gtattaatta
     3361 gtatttttag gagaatgaaa gatctttatg cagctggtta tgattttgga gatattcgtt
     3421 atttaaagcc gtttcttgat ggacatgtcg atgaaaatga ttatactaaa ttagatgagg
     3481 ggatcgtttt ttattatttt acagtcttaa aagaaggcaa tgacgagatt ttgaaagatt
     3541 tatgtacacg ctttttagat cgacgattgt ttatttatca tgatctgctc gatcaacatg
     3601 aaaaacaatt agctgaatca ttttatgaaa aaaaaggcta tgatcctcgt tattatgttg
     3661 taagtgatga tcaaagccaa gttccctatc gttattatgg aaatactgaa gaactcagtg
     3721 aaattgaaat attaattgat gaagaactcc ggtttttacc agaagtatca gaaattgttg
     3781 gggcaattgt aaattctaag aaaaataaaa atgatcataa gattttctat cccgaagtgt
     3841 aaacttcggg attagtcttt tttaaaccaa tagactgcac cattttcatc aaaatcaccg
     3901 gttagtggat tgattgcaat ttgctgtagg tcttcggttg gctgatacca tgtaacatct
     3961 ttgttttcat tggtatagtt taaaacatcg ataacggttt ttttagcaac agttttgtca
     4021 tcagctgttt ccattttacg attgtcatcg tagccgaccc agctggcaac caggacttgt
     4081 ggattataag ctacagccag gttatcaaca tcggtactac cagttttaca ggcgttgata
     4141 ttatcaagtt gataagaagc cattgttgct tgaagatagg tactaaatgt actattaaat
     4201 gtagatgtta atagctgtga taattctaaa caactatctt gctccagcaa ttgtttatct
     4261 tcttgttcgt gctggtaaag gactttacca gcatcattag taattttttc aattgtatag
     4321 agatgattgt attttccttc actagcgaga caattataca tattggcaag attatatatg
     4381 tttgtattta aagtcccaag tgccagtgag gcatttgctt tgacgtcctt gattccaaat
     4441 tttttgatta atgaagctaa gttatcagtt cctaaaaata gatgtgtttt aacggcgaaa
     4501 atattatcag atacagcaat agcttgagct aacgtaacat ctttatatgc atatttatcg
     4561 ttaaaattac taggactgta agtgctgtta tcgtcaagtt taaaggtagt aggttcacat
     4621 aaaaatgtgg ttgccggagt gaaaccgttt tctagcgcca ggtagtataa caaaggtttg
     4681 attgtactgc cgatttgtcg attagcttga gtggcacgat tgaattgtga gctggcatag
     4741 tctttaccgc cgataatagc aaggatttta ctggtatatg gttcaacaac gatactcgag
     4801 gtttcgactt tactatcctt agtatattca ctagcacttt tttccactac tttttggtaa
     4861 tcttgattaa aacttgtata cacatttaaa ccttgattca aatatgtatt gttgtagtaa
     4921 ccaagttctt cgagttcatc aataacggta tctttatagt aattattagc catattatca
     4981 tctactttat gcgtttcagc aagattaaga tcactatcaa gaacctgctg tttttgagtt
     5041 acggtaattt ccttacagtc gattagagcg tttaaaacga ttgactgtcg ttttcgagct
     5101 gctttagcat catttaaggg tgagtaatag gttgggccat taactactcc agccaacata
     5161 ctgctttcat ttaaatctag ttctttagca cttttaccat aaaaatattg tgcagcattt
     5221 tcaataccat aaattccatg accaaaataa acgttattaa tatatccctc gagaatttca
     5281 tcttttgata aacgtgtttc taactgcatt gtcaaaaaag cttctttaat ttttcgcgac
     5341 caggattttt cgttagttaa gtacaataag cgtgcatatt gctgagttat cgtactcgct
     5401 ccttgagatt ttttttgatt tgtgaagttt actttgaggg ctctcatgat tccaattgga
     5461 tcaaaacccc gatggtcata aaagcgttga tcttcaatag ctacaatact ttctttaaaa
     5521 taattactaa cattatttaa agttacataa tgaccggtat agtcgttgat cgactgataa
     5581 taaatttctt gattatcatc gtataatttt aaataacgat tcttgtttag atccggttcc
     5641 cccatgcaat atgcaataac atatagtgat agaagtatgc cggctaaact tgctaaaata
     5701 gttacaaata tttttactaa ttttttcatc atatcaccaa aagtattctt gacggatttg
     5761 tgttataata tgcacggtga tagaatgaaa aaaataatta aagtaaatta tcgaagtatt
     5821 tttaaatatg aagaacatca agaaactgtt aaatatgatg gttctggtca tctggagata
     5881 ggtgatgata aaattgtagt cagttatcaa gatgaaaata aaataaaaat tgaattaagt
     5941 gaaaatgaag ttaaacttca taatggggct agtgttttgc atttagtaag agatcgtgat
     6001 attttaaatc aatatgaaac tccatatgga gcaattgctt taaaaactag attaattagt
     6061 tatgataacg gggataatgt gaaaataaag tatgaattat atgatggaac taatttgatt
     6121 agtcaagtat atgtcatgct caattattta atattggaga actaagatga aaatactaaa
     6181 gtttttatca aaccggatcg ttgtatcgat tattttaata tgtgttcaga tattatggct
     6241 agctttattt atgcgctata tttttactga tgcgacctta ttaaaaggag tttttatttt
     6301 tttaagtgtg atcgtaactt tatatattat caacaatgac gatgatgacc ccagttataa
     6361 gattatttgg ttaattccca tcttatcttt tccggttttt ggtggtttgt tgtatgttgt
     6421 ttttggtaat aaaaagccag ctaaaaagct tcaagaagcc tttgctaatc aagaagaagc
     6481 tattagacca tttatttatc caaataagat ggttgaacaa attgaggatt tgattgcaaa
     6541 aggacaagct aattatttag ttcaagaaaa ttttcctatt tataataata gtgagattaa
     6601 atattaccct ttaggtgatg atacatatcc agatttgttg aaagaattag ctaaagcaaa
     6661 acattttatt tttatggaat attttattgt tgaagaaggt gagatgttta atacagtctt
     6721 aacgatatta aagcaaaaag ttaaagaggg tgtggaagta cgatttatgt atgatgatat
     6781 gggctctttg acaatgctgc catttcgtta ttatcaaaaa ttggaaagtt atggaatcaa
     6841 gtgtatagca tttaatcatt ttgttccatt tatttcggca gtaatgaata cccgtgatca
     6901 tcgtaaaatt acggtgattg atggaaatgt tggttttagt ggtggtttta atctggctga
     6961 tgagtatatt aatgctaaag tgaaatatgg acattggaaa gatacggggg taatgatcaa
     7021 gggtgaggct gtatggaatt taacactgat gtttctaaca acgtggaatg catcattaaa
     7081 tacatttgaa gattatgata aatatcatcc tcgtcattat atatgtcccg aaataagtcc
     7141 aaatgggtat attttaccat acggcgattc accattggat aataagccgg tggggaaaaa
     7201 cgtttattta aatatgatta atcaagcaca aaaatatatt tatatt
//
LOCUS       DAOMTA010000040         7243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_134_length_7243_cov_2.697830, whole genome shotgun sequence.
ACCESSION   DAOMTA010000040 DAOMTA010000000
VERSION     DAOMTA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_7243_cov_2.697830"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLK68_01960"
     CDS             complement(<1..1051)
                     /locus_tag="PLK68_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRM89204.1"
                     /translation="MFKDYVLSLKKEFAGYNGQKLVMDILAGLTVAAVALPLALAFGV
                     SSGADAGAGLITAIIAGLLIGGLSGASYQISGPTGAMSAILIGLSTTYGLQGVFVASF
                     ISGVMLLIASLFKFGKIVSFIPASVITGFTSGIAIIIATGQIDNFFGVTSKGSNPIEK
                     ILSYRMLGFNVNLEALFFGILVIAIIILWPKKWGNIFPASLAGIIIALIINLVFKFNV
                     AEVGSIPSTLLPKARLSLNSLNLTAITNLIIPAFSIAMLGMIESLLCGASAGKMKNEK
                     LNADQELFAQGIGNMVIPFFGGVPATAAIARTSVAIKAGGQTRLVSIFHAAALLISMF
                     VLGPFMSRIPLSALAG"
     gene            complement(1275..2141)
                     /locus_tag="PLK68_01965"
     CDS             complement(1275..2141)
                     /locus_tag="PLK68_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM89205.1"
                     /translation="MNLRHLLIFKTVVDTGSFTKAAKQLFITQSGVSHAIRELEQQTN
                     AVLFDRLSKAIILTPAGKLLLEKVIPILSLYHDLEKQIDVLEMSAPLKVVSSITIATF
                     WLPKILKQFASSYPNIKVEVQVVSAKEALAVLECGEADLALVEGVVPPGPFIVIDFSS
                     YQLNVLCAPDYFADNEITLKELCTHDLLLREKGSATRDIIDSCLYLRGLAAYPKWTSV
                     NSKVLIEACKAGFGFAVLPTLLVTEELKHGTLKTVETDELLYNKTKLLYHQEKYISEP
                     LAKLIDIIGEAE"
     gene            2246..2815
                     /locus_tag="PLK68_01970"
     CDS             2246..2815
                     /locus_tag="PLK68_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM89206.1"
                     /translation="MKANKLKDLTWLFFINIFISSFTFGGGYVVVSMVRKYFVEKRKI
                     FNESDLITMSAISQTTPGAIAINLAALAGYKVAGTIGTIVSCIGAIIPPITILAVVSL
                     WYQVFSTNHIIMAILKGMQAGIAAIIVDILIGMTRAINEQHSKLLTAMIPITFIASYI
                     FKVNIVVIILITIIVATAQLYYRRRYCQC"
     gene            2809..3336
                     /locus_tag="PLK68_01975"
     CDS             2809..3336
                     /locus_tag="PLK68_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM89207.1"
                     /translation="MLNLFIIFFKIGLFSIGGGYAIIPLIQEQVVNNYHWISANTFTD
                     IITISQMTPGPLAVNTSTFVGIQLAGISGAIVATIGCIFAGVTISIILYLLFTKYQKL
                     EIITNILNTLKATSVGLIMSAGATILILTFVENNSINFLAIIIFVISLLWLQKKKPNP
                     IALMIITGIIGYFVY"
     gene            3977..>5739
                     /locus_tag="PLK68_01980"
     CDS             3977..>5739
                     /locus_tag="PLK68_01980"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HRM89208.1"
                     /translation="MNTNQKILIVGGVAGGASAAARLRRLDESANIIMFEKDEYISFA
                     NCGLPYYIGGEITDQEALTLQTPDSFKARFNVDVRNFNEVTAITPETKTVTVYNHQLQ
                     KEYQESYDKLLLSMGAKPIKPNIPGINSNKVFTLRNIPDTYAIKEYVDTHKPKHAIVV
                     GGGFIGIEMAENLHSTGINVTIVEMANQVISPIDYEMACEVHQHLISKGINLVLETEL
                     QAINEAGNKLTVTLNNQTVDTDMVIMAIGVVPETKIVKKTEIATNSRGAIIVNDKMET
                     SIKDIYAVGDAIEIKNFVTNKASYVPLAGPANKQGRIAADNICGFDRHYQGTQGSSIL
                     KVFDLTVASSGINEKTARELNLNYDKVYTYSANHAGYYPGAVNMSIKVLFDKSTGTIL
                     GAQIVGYDGVDKRMDVLAAAIRAKMTGFDLTELELCYAPPYGSAKDPVNMAGFVIENI
                     LTDKIKQYNWDDVASLPRDGSVILLDTRTELEYANGHIDGYINIPLDSLRTRLHELNL
                     NKPIYVTCQIGLRGYIASRILSQNGFDTYNLNGGYRLYNTIFNQEHDEPKIKTMHPAC
                     PIENPETIKINACGLQCPGPIV"
     assembly_gap    5740..5749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5943..6398
                     /locus_tag="PLK68_01985"
     CDS             5943..6398
                     /locus_tag="PLK68_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016716529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRM89209.1"
                     /translation="MIVFSGDLDKAIASFIIANGAAAMGRKVTMFFTFWGLNIIRRPE
                     KVKIKKNFISKMFAMMMPRGSKKLSLSKMNMGGMGAKMIRTIMKDKNIDSLEDLIKLA
                     QDNGVELIACSMSMDVMGIKQEELIDGVTLSGVATMLANGEESDMSLFI"
     gene            complement(6434..>7243)
                     /locus_tag="PLK68_01990"
     CDS             complement(6434..>7243)
                     /locus_tag="PLK68_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM89210.1"
                     /translation="VYGTTLKSKLQEIYKKYKDLDISSYTPNSIARYQEALNNAVKVY
                     NDDDVTSEDILKAIDLLEDSVKGLTKIVDKMELEETINSASKQLDTAKYTPQSIELLT
                     VTLQKAKEVFNDDNATKHEVDEAINKLNEVVAALVEKADKNNLISIFNTALKLDKEKY
                     TSESLTKVEALLEKVESIIDDENAAQAEVDEMYDQLHQALDQLVIKEDVNTNDKVEGD
                     VTVIPTPEKEIPKDDSTSAAKTGDSVSIQILAGTLLISILGIGILRKKKQS"
BASE COUNT         2499 a         1245 c         1058 g         2431 t
ORIGIN      
        1 caccggctaa agctgataaa ggaattcttg acataaatgg acctaaaaca aacattgaaa
       61 ttagtaaagc cgctgcatga aaaatactaa ctaaacgcgt ttgaccgcca gctttaatcg
      121 caacactcgt tctagcaata gcagctgtag caggaacacc tccaaaaaaa ggaataacca
      181 tattaccaat cccttgtgca aaaagttctt gatctgcatt taacttttca tttttcattt
      241 ttccagcact agcaccgcat aaaagtgatt caatcattcc taacatagca atactaaatg
      301 caggaataat tagatttgtt attgctgtta aatttaaact attcaatgac aaacgagctt
      361 taggcaataa tgtagatgga atactcccga cctctgctac attaaattta aatactaaat
      421 taataatcaa agcaataata attccagcta atgaagccgg aaatatattt ccccattttt
      481 taggccataa aataataatt gctattacta gtatcccaaa aaacagtgcc tctaaattta
      541 cattaaagcc taacatacga taagataaga ttttttcaat cggattgctt cctttcgaag
      601 taactccaaa aaaattatca atttgtccag ttgcaataat tattgcgatt ccactcgtaa
      661 atccagtaat tacactagct ggtataaatg atactatctt accaaactta aataatgaag
      721 caattaataa cattacccca gaaataaaac tagcaacaaa caccccctgt aaaccatatg
      781 tagttgacaa tccaattaaa attgctgaca ttgctccagt tggtccagaa atttgatacg
      841 atgccccaga taagccaccg atcaataatc ctgctataat cgctgtaatc aaaccagccc
      901 cagcatcagc accactactc accccaaaag ccaaagctaa cggcaacgca accgcagcta
      961 cggttaatcc tgccaaaata tccattacta gcttttgacc attataacct gcaaattctt
     1021 ttttcaaact taatacataa tctttaaaca tcctaccctt tatcctttca aaatactttt
     1081 ttattataat gataaatatt agtgatttaa agtaaaaaat aaatatttta ctgtttttta
     1141 ttaatatttc taacttatta taaaaatatc gataaaatca tcaaccataa aaagactact
     1201 acttctactc taaatacatt aaaagaggct ataacaccta aaagtttaaa aaactttatt
     1261 ccttatagcc tcttttattc agcctctcca ataatatcaa ttaatttagc caatggttca
     1321 cttatatatt tttcttgatg atataaaagc tttgttttat tataaagcaa ttcatctgtt
     1381 tccaccgttt ttaaagtacc atgctttaac tcctcagtaa ctaaaagagt aggtaaaacc
     1441 gcaaaaccaa atccagcctt acatgcttca atcaggacct ttgaatttac acttgtccat
     1501 ttaggatatg ctgccaatcc tcgcaaatat aaacaactat caataatatc tctagttgca
     1561 ctcccttttt cacgaagcaa aagatcatga gtacaaagtt cttttaatgt aatttcatta
     1621 tccgcaaaat aatctggtgc acataaaacg tttaattgat aagaagaaaa atctattaca
     1681 ataaagggtc ctggtggaac tactccctct accaaagcaa gatcggcttc tccacattcc
     1741 aaaactgcca atgcttcttt tgcactgacc acttgcacct caaccttaat attaggatag
     1801 ctgcttgcaa attgttttag aatctttggt aaccaaaagg ttgcaatagt aatacttgaa
     1861 acaactttta gaggcgcact catttccaaa acatctattt gtttttctaa gtcatgatat
     1921 agtgataaaa ttggaataac tttttccaat aataatttcc ctgcaggagt caatataatt
     1981 gcctttgata aacgatcgaa taggacagca tttgtttgtt gttctaattc cctaatcgca
     2041 tgcgatactc cagattgcgt aataaataat tgttttgctg ctttagtaaa actccctgta
     2101 tcaactactg ttttaaatat taataaatgt ctaaggttca tagctttctc ctatcatgaa
     2161 taaaagtaat gtatttcatt aaattatatc attttactaa tgtatataca acttatataa
     2221 tcttgatata taaaggagaa tattaatgaa agcaaataaa ctaaaagacc ttacatggtt
     2281 gttctttatc aatattttta ttagcagctt tacatttgga ggtggttatg tggttgtatc
     2341 aatggttcgt aagtattttg ttgaaaaaag aaaaattttc aatgaaagtg acttaatcac
     2401 catgtcggca attagccaaa caacacctgg agcaatcgca atcaatcttg cggcattagc
     2461 tggttataaa gttgccggta ccattggaac tatcgttagt tgcattggag cgattattcc
     2521 gccaattact attttggcag ttgtttcatt atggtatcaa gtttttagta caaatcacat
     2581 cattatggct atcttaaaag gaatgcaagc cggaattgct gctattatcg tggatatcct
     2641 aataggaatg acaagagcta ttaatgaaca acattcaaaa ttattaaccg cgatgattcc
     2701 tattactttc attgccagct atatctttaa agttaatatc gttgtaataa tcttaattac
     2761 aattatcgta gccactgctc aactatacta tcgccggagg tactgccaat gttaaattta
     2821 tttataattt tctttaaaat tggattattc agtattggtg gcggctatgc aatcattcct
     2881 ttgattcaag aacaggtcgt caataactat cattggatca gtgccaatac atttactgac
     2941 attatcacaa tttcgcaaat gacacctggc cctttagcag ttaatacctc tacttttgtg
     3001 ggtattcaac tagctggaat tagtggtgct atcgttgcta caattggttg tatttttgct
     3061 ggagtaacaa tttcaattat cctttatctt ctttttacta aatatcaaaa actagaaata
     3121 attacaaata ttctaaatac tttaaaagct acatcagttg gtttgatcat gtctgccggt
     3181 gcaacaattt taattctaac ttttgttgaa aataactcta tcaacttttt agcaataatt
     3241 atttttgtaa tttctttatt atggttacaa aaaaagaaac ctaatccgat tgccctaatg
     3301 attatcacag gaattattgg atatttcgta tattaagaca aaacccggtt actaaatcta
     3361 ataatgacct ttgatttctt atttattcag ctagttctaa aatgcctaac taaagattat
     3421 ctttatttcc aatgaacatt ttaacaaaat caaaaaaaac tgcctatatg acattaagaa
     3481 gtgtctaata tgaatggttt tggtcttgtc taatttaata tcaacaatcc attttttaca
     3541 ttacatcaac aaattagtca atcaatttaa tatagataat tatctagctt ctaaattact
     3601 ttattaataa cgctgatatt catatcctaa actctaatta attattctga atagataaaa
     3661 ataattgtat tttttattac aaatatttat cacttctttg ataattatca aaatatttag
     3721 ttttagtata gctttcctaa cattattgaa agataacaat cttacttcta atataaatca
     3781 atacttgact tcattgaata ttgaagataa taaatgtata cataattcaa aatacatgaa
     3841 tatctcatta ttaaataatt ttccttacta aaccactatt cttataattg tgtgacctag
     3901 ttacagaact aattttaaat taaaattata ataatcttga aaatgaatca tagattaata
     3961 attatggagg aaaaaaatga atactaatca aaaaattctt atcgtaggcg gagtcgctgg
     4021 tggcgcttca gctgctgctc gtctaagacg tttagatgaa tctgccaaca ttattatgtt
     4081 tgaaaaagat gaatatatat catttgctaa ctgtggttta ccctattata ttggtggtga
     4141 aatcactgat caagaggctt taaccttaca aactcctgac agttttaaag cacgctttaa
     4201 tgtcgatgtt cgcaacttta acgaagttac tgctattact cctgaaacta aaaccgttac
     4261 tgtatataat catcaattac aaaaagaata tcaggaaagt tatgataaat tacttctttc
     4321 aatgggagca aaaccaatca agccaaatat tcctggaatc aattcaaata aagtttttac
     4381 acttagaaat atccctgata cttatgcaat aaaagaatac gttgataccc ataaaccaaa
     4441 acatgcaatt gttgtaggtg ggggatttat tggtattgaa atggcagaaa atttacattc
     4501 aactggaata aatgtaacga ttgtagaaat ggctaatcaa gtcatttctc cgattgacta
     4561 tgagatggct tgtgaagttc atcaacatct tataagcaaa ggaattaact tagttttaga
     4621 aacagaactt caagctatta atgaagccgg taacaagctg acagttactc taaataatca
     4681 aactgtagat acagatatgg taattatggc aattggtgtg gtacctgaaa ctaagattgt
     4741 aaaaaaaaca gaaattgcaa ctaattctcg cggagcaatc atcgtcaacg ataagatgga
     4801 aacatcaatc aaagatatat atgctgtagg cgatgctatt gaaatcaaaa attttgttac
     4861 taataaagca agttatgtac ccctagcagg cccagctaat aaacaaggac gaattgcagc
     4921 tgataatatt tgcggttttg atcgccatta tcaaggaaca caaggttcat caattttaaa
     4981 agtctttgac ttaactgttg caagtagcgg aatcaatgaa aaaactgcta gggaattaaa
     5041 tctcaattac gataaagtct acacatattc agctaatcat gccggctatt atccaggtgc
     5101 ggtcaatatg tcaataaaag tcctttttga taaatcaacg ggaaccattt taggggctca
     5161 gatagttggt tatgatgggg ttgacaagcg catggatgtt ttagcagctg ctattcgtgc
     5221 caaaatgacg ggttttgatt taactgaatt agaactttgc tatgctccac catatggatc
     5281 agcaaaggat cctgttaaca tggctggttt tgtaattgaa aatattctta ctgacaagat
     5341 caaacaatat aattgggatg atgttgcatc actgccccgt gatggcagtg tgattctatt
     5401 agatactcgt actgaattag aatatgcaaa tggtcacatc gatggttaca ttaatattcc
     5461 attggattca ttgcgaactc gtcttcatga gcttaattta aataaaccta tttatgtaac
     5521 ctgccagatt ggacttagag gctatatagc atcacggatt ttaagtcaaa atggttttga
     5581 tacttataat ctcaatggag gatatcgctt atataataca atttttaatc aagaacatga
     5641 cgaacccaaa atcaagacaa tgcatcctgc ttgtcccata gaaaatccag aaacaattaa
     5701 aattaatgcc tgcggtcttc agtgtccagg tccgattgtn nnnnnnnnna ctagaaactg
     5761 ctaaagatgg cgatattatt gaaattcaaa ctacggaccc ggcttttgct acagatctag
     5821 atggctattg tcgtcgtact ggtaatgaac taatagaact tagctgtaat aaaggaatta
     5881 gcagtgctaa aataaagaaa ggacagaaca gtatctcaaa cggaaataaa aataataaaa
     5941 atatgattgt tttctctgga gatttagata aagcaattgc atcatttatt attgctaatg
     6001 gcgcagcggc tatgggacga aaagtcacaa tgttctttac cttctgggga ctaaatatca
     6061 ttcgacgacc agaaaaggtt aaaatcaaaa agaattttat ttcaaaaatg tttgcaatga
     6121 tgatgcctcg tggttcaaaa aaactatcgc tttcaaaaat gaatatgggt ggtatgggag
     6181 ctaaaatgat ccgtactatt atgaaagata aaaatattga cagtttagaa gatttaatca
     6241 aactagctca agataatggt gttgaactaa ttgcctgttc gatgagtatg gatgtaatgg
     6301 gcattaaaca agaagaattg attgatggtg ttacattatc gggagttgct actatgttag
     6361 ctaacggtga agaatctgat atgtcactat ttatataata aaaggagctt ggaattaatt
     6421 ccaagtctcc tttttatgat tgtttctttt ttcttaaaat tccaattcct aagatgctga
     6481 tcaataatgt tcccgccaat atttgaatag atactgaatc accagtttta gctgctgaag
     6541 tactatcatc ttttggtatc tctttttctg gcgtaggaat aactgttaca tcaccttcaa
     6601 ctttatcgtt tgtatttaca tcttctttta tcactaattg atctaacgct tgatgcaatt
     6661 ggtcatacat ctcatcgact tctgcctgtg cagcattttc atcatcaata attgattcta
     6721 ccttctctaa caaggcttca actttagtaa gactttcaga agtatatttt tctttatcaa
     6781 gtttcaatgc agtattaaaa attgatatta aattattttt atcagctttt tcaactaatg
     6841 ctgcgactac ttcatttaat ttattaattg cttcatcaac ttcatgtttc gtagcattat
     6901 catcattgaa aacttcttta gctttttgta aagtgactgt aagtaattca attgattgag
     6961 gggtatattt agctgtatct aattgtttac ttgctgaatt gattgtttct tccaactcca
     7021 ttttatcaac aatcttagta agtcctttaa cactatcttc caacagatca attgctttta
     7081 agatatcttc agatgtgaca tcatcatcgt tataaacttt aacagcatta tttaatgctt
     7141 cttgatatcg cgcaatacta ttaggggtgt atgatgaaat atctaaatct ttatatttct
     7201 tataaatttc ctgtaactta gattttaaag ttgttccata aac
//
LOCUS       DAOMTA010000041         7142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_142_length_7142_cov_2.718358, whole genome shotgun sequence.
ACCESSION   DAOMTA010000041 DAOMTA010000000
VERSION     DAOMTA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_7142_cov_2.718358"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLK68_01995"
     CDS             <1..578
                     /locus_tag="PLK68_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89211.1"
                     /translation="EKFGKIDNDILLPLADHIYFAIKRMDENIMPLNPFINDIRLLFP
                     DEYEVALEGREVINKFISRMINDDEVGFITLHIHSAISSNQVAESMEATRIIHESIIK
                     LENDLNIVIDIQSVSYARLMNHIKFLILRLNKNEELQMDISEFTKDKFPFAYEQASNM
                     CEALSKVLKKKLPETEVGYLALHLERILSSI"
     gene            801..1790
                     /locus_tag="PLK68_02000"
     CDS             801..1790
                     /locus_tag="PLK68_02000"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HRM89212.1"
                     /translation="MVGIILASHGEFANGILQSGAMIFGDQVDVKAVTLQPSEGPDDL
                     KAKMEAAIATFENQDEVLFLVDLWGGTPFNQANGLIAGHEDKWAIVTGLNLPMLIETY
                     ASRMSMETAHEIAKHVTEVAREGVKVKPEELEPETKVAAQAINTQSQGAIPEGTVLGD
                     GHIKYVLARIDTRLLHGQVATTWTKTTQPNRIIVVSDAVSKDKLRKQMIEQAAPPGVK
                     ANVVPIDKMIQVAKDPRFGNTKAMLLFETPQDALRAIEGGVDIKELNIGSMAHSIGKV
                     VVNKAIAMGPEDIETIEKIKAKGITFNVRKVPSDSNENIDTLLKKAKAELKNV"
     gene            1816..2631
                     /locus_tag="PLK68_02005"
     CDS             1816..2631
                     /locus_tag="PLK68_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HRM89213.1"
                     /translation="MSLITIILIIIIALLAGMEGILDEFQFHQPLVACTLIGLVSGHL
                     TEGIILGGSLQMIALGWANVGAAVAPDAALASVASSIIMVLGLEGGATDVQTAISTAI
                     AVAIPLSVAGLFLTMICRTLTIPIVHFMDGAAEQGNMRAIDMWQILAILLQGIRIAIP
                     AAALCVVPAAVVTDALNQMPPWLSGGMTVGGGMVAAVGYAMVINMMSTKETWPFFALG
                     FVLAAIGELTLIALGAIGVALAIIYLGLKENSGSNGGGSTGSGDPLGDILNDY"
     gene            2655..3572
                     /locus_tag="PLK68_02010"
     CDS             2655..3572
                     /locus_tag="PLK68_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM89214.1"
                     /translation="MAEKIKLSKKDRMSVAWRHQFLQGSWNYERMQNGGWCYSIIPAI
                     KKLYPNKEDKVAALKRHMEFYNTHPYVSAPVMGVTLALEEERANGAEINDTAIQGVKV
                     GMMGPLAGVGDPVFWFTLRPILGALGASLALSGNIIGPLIFFFAWNIIRIAFLWYTQE
                     FGYKVGTSIAQDLSGGLLGKITQGASILGMFIIGSLVQRWVSITFTPVVSTVTQSKGA
                     YIDWNSLPSGVDGIKSALEQFASLGSNGLNVEKVTTLQQNLDQLIPGLSALLLTLLCC
                     WLLKKKVSPIVIIVALFIIGILARLGGIM"
     gene            3615..3998
                     /locus_tag="PLK68_02015"
     CDS             3615..3998
                     /locus_tag="PLK68_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HRM89215.1"
                     /translation="MVQSLNTKVDLTVKATSYLGLANYGEVMVGDKAFEFYNEKNIRD
                     YIQIPWEEVNCVMASVMFKGKWIPRFAVVTKNNGNFTFSSRDNKALLRAINKYIPSEN
                     LVRSLSFFQVIKRGLKSLFRKKSRQ"
     gene            complement(4099..4947)
                     /locus_tag="PLK68_02020"
     CDS             complement(4099..4947)
                     /locus_tag="PLK68_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89216.1"
                     /translation="MADSKIIFIDVDGTLVDYENKLPASADKAIKQARKNGHRVYICT
                     GRSKAEVYPYLWDIGLDGMIGGNGSYVEDGDTVVMHQLITAEQCKHIVDWLKSRGLEF
                     YLESNNGLFASENFETRGEPVIQEYSKRKGKEHSDQIKVRDVFPEMIFDGELYRDDLN
                     KVSFILESYQDHLDSIEEFPDLKAGTWGGAGEIALFGDLGVKDITKAHAIDALLEYLG
                     ADIEDTYAFGDAKIDIPMLDYCHIGVAMGSGGDEIKAMADYITDDVDQDGLYNAFVRF
                     GLIEAE"
     gene            complement(5221..5841)
                     /locus_tag="PLK68_02025"
     CDS             complement(5221..5841)
                     /locus_tag="PLK68_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM89217.1"
                     /translation="MRSKKTQYMTFMAMFLAIEIILVVTPLGYIPIGPLSATTMHIPV
                     IIAGITLGKKAGAQLGLVFGLTSLIRATIQPGITSFCFSPFVTVGNISGDWRSVIIAL
                     VPRILLGYLAGVIFEFIKNKFNNENAAAVIGALIGTITNTVLVLGGIYFFFGTAYADA
                     VNIAYSSLLAMLFGVVTTNGIVEALIGAVVTLLAYKAIKPMATNIK"
     gene            complement(6010..6978)
                     /locus_tag="PLK68_02030"
     CDS             complement(6010..6978)
                     /locus_tag="PLK68_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRM89218.1"
                     /translation="MKKINITEIKGIQIGQVENQKAGTGCTVIICKDGATAGVDVRGG
                     GPATRETDLLNPINMVQQINAVMLSGGSAFGLDAASGVMQYLEEHNCGFDMKVAHVPI
                     VCGASLFDLTVGDPKIRPDKAMGYQACLNSEKNEPLKEGNYGAGCGASVGKIMGPQYA
                     MKGGLGTAAIQIDNLQVGAIVAVNACGNIVDYKTNEQLAGIYDSEHNSIIPADEVIFQ
                     QIEKLRQLPSGNTTIGCIITNAKLNKAQCTKIAGIAHNGYARAIQPVHTMSDGDTIFV
                     LSTNEVEVMPDAIGILATDLMAKAVNRAVKKADSAYGLKSYKELHK"
BASE COUNT         2371 a         1174 c         1272 g         2325 t
ORIGIN      
        1 tagagaaatt tggaaaaatt gataatgata tcttattacc attagcggat catatctatt
       61 ttgcaataaa gcgaatggat gaaaatataa tgcctttaaa tccatttatc aatgatattc
      121 gtttgctgtt tcctgatgaa tatgaggttg cattagaagg acgggaagta attaataagt
      181 ttatatcacg aatgatcaat gatgatgaag ttggctttat tacactacat attcattcag
      241 caatttcttc taatcaagtt gctgagtcaa tggaggcaac acgaattatt catgaaagta
      301 ttattaaatt agaaaatgat cttaatattg ttattgatat tcaatcagtt tcatatgctc
      361 gattaatgaa tcatattaaa tttttaattt taagattaaa taaaaatgaa gaattgcaga
      421 tggatataag tgaatttact aaagataagt ttccttttgc atatgaacag gcaagtaaca
      481 tgtgtgaagc attatcaaag gttttaaaaa agaaattacc agagacagaa gtaggatatc
      541 tggcattaca cttggaaaga atattgtcaa gtatttaaaa atatattatt ttgaaataat
      601 aatttttaaa tgatttcttg acagaaaagg cttacaatga taatattgtt tcataaattg
      661 aatattttat agtgtgtaac tgataaagca ggcattaact atttaaaaaa agaacaaaga
      721 tggtattttt gtatctttta ataatagttt gcctgttttt ttgcgttata aagtatttaa
      781 aagaaaggga ggaatttata atggtaggaa ttattcttgc tagtcatggt gagtttgcta
      841 acgggattct acaatccggg gcaatgatct ttggagatca agtcgatgta aaagcagtta
      901 ctttgcaacc aagtgaagga cctgatgatc ttaaagcaaa aatggaagcg gcaatagcta
      961 cttttgaaaa ccaagacgaa gtactatttt tagttgattt atggggtggt acaccattta
     1021 atcaggctaa tggattaatt gctggtcatg aagataagtg ggcaatcgtt acgggtttaa
     1081 atttaccaat gctgattgag acttatgctt ctagaatgtc gatggaaaca gcgcatgaaa
     1141 ttgctaaaca tgttactgaa gttgctagag aaggggtaaa agtaaaacct gaagaattag
     1201 aaccagaaac gaaagtagcg gctcaagcta ttaatacaca gtcacaagga gctattcctg
     1261 agggaactgt attaggggat ggacatatta aatatgttct ggcacgaatt gacacgcgtt
     1321 tattgcatgg tcaagttgca actacttgga ctaaaacaac acaaccaaat cgaatcatcg
     1381 ttgtttctga tgctgtttca aaagataaat tacggaaaca gatgattgaa caagccgcgc
     1441 caccaggagt taaggctaat gttgttccaa ttgataaaat gattcaagtg gctaaagatc
     1501 cacgttttgg taataccaaa gcaatgttat tatttgaaac acctcaagat gcattacggg
     1561 caatcgaagg cggtgtagat attaaagaat taaatattgg ttcaatggct cattcaattg
     1621 gtaaagtggt agtaaataaa gctattgcaa tggggccgga agatattgaa acaattgaaa
     1681 aaattaaagc taaaggaatt acttttaatg ttcgtaaagt accaagtgat tcaaatgaaa
     1741 atattgatac gcttttaaag aaagcaaaag cagaattgaa aaatgtttaa aaataaaatg
     1801 tagggaggaa ttataatgtc tttaattaca ataatcttaa ttattataat tgccttacta
     1861 gcggggatgg aaggaattct ggatgagttc caatttcacc aaccgttagt ggcatgtact
     1921 ttaatcggtt tagtaagtgg tcacttaact gaagggatta ttctaggtgg ttcgcttcaa
     1981 atgattgcat tagggtgggc caatgtaggg gctgctgtag caccagatgc agcattagct
     2041 tctgtagctt catcaattat tatggtatta ggtttagaag gtggagcaac tgatgtgcaa
     2101 acagctattt ctactgcaat cgcagttgca atccctttat cagtagcagg tttattctta
     2161 acaatgatct gtcgtactct tacaatccca attgttcact ttatggatgg ggcagccgaa
     2221 caaggaaata tgcgtgcaat cgacatgtgg caaattttag caatcttatt acaaggaatt
     2281 cgaattgcaa ttcctgctgc agcactttgt gttgttcctg cggcagttgt aactgatgca
     2341 ttaaatcaaa tgccaccatg gctatctggc ggaatgactg ttggtggtgg aatggtagcg
     2401 gcagttggtt atgcaatggt aatcaatatg atgtctacta aagaaacatg gccattcttt
     2461 gcattaggtt ttgttcttgc tgcaatcggt gaattaacat tgatcgcatt aggagcaatt
     2521 ggtgttgctt tagcaatcat ctatttagga ttaaaagaaa acagtggctc taatggtggc
     2581 ggttcaacag gttctggaga tccattaggc gatatcttaa atgactatta aaatcttgaa
     2641 ggaggaaaga aattatggca gaaaaaatta aattatcaaa aaaagatcgt atgtctgttg
     2701 cttggcgtca tcagttcctt caaggttctt ggaactatga acgtatgcaa aatggtggtt
     2761 ggtgttattc aattatccca gcaatcaaaa aattatatcc aaataaggaa gataaagtag
     2821 cggctttaaa aagacatatg gaattttaca atactcatcc atatgtatca gcgccagtca
     2881 tgggtgttac attagcatta gaagaggaac gtgctaatgg agcagaaatc aatgatactg
     2941 caatccaagg tgtaaaagtt gggatgatgg gtcctttagc tggggttgga gatcctgtct
     3001 tctggtttac tcttagacca attttaggag cattaggtgc ttctctagca ttaagtggta
     3061 atattattgg tccattaatc tttttctttg catggaatat cattcgtatc gcatttttgt
     3121 ggtatacaca agaatttgga tataaagttg gtacttcaat tgcacaagac ttatctggtg
     3181 gattattagg taaaattact caaggtgctt caattcttgg gatgttcatt atcggatcat
     3241 tagtacaacg ttgggtaagt attactttta ctccggttgt atcaactgtt acgcagtcaa
     3301 agggcgcata tattgattgg aatagtttgc cttctggtgt agacgggatc aaatcagcat
     3361 tagaacagtt tgcatcatta ggatctaatg gtttaaatgt tgagaaagtt acgaccctac
     3421 aacaaaattt agatcaactt attccaggtt tatcagcatt attattaaca ttattatgct
     3481 gttggctatt gaaaaagaaa gtatcaccaa tcgttattat cgttgcttta ttcattatcg
     3541 gaattcttgc acgtcttggt ggaataatgt aatataataa agaaaataca tggcagctgt
     3601 aaaggaggaa atatatggta caatctttaa atactaaagt agatttaaca gtcaaagcaa
     3661 cttcttatct tggtcttgct aattacggag aagtcatggt aggcgataaa gcatttgaat
     3721 tctataatga aaaaaatatt agagattata ttcaaattcc atgggaagaa gttaattgtg
     3781 taatggcttc agttatgttt aaaggaaaat ggattcctcg ctttgccgtt gttaccaaaa
     3841 ataatgggaa ttttactttc tcatcacgcg ataataaagc attattacgt gctattaata
     3901 aatatattcc ctcagaaaat ttagttcgat cacttagttt ttttcaagta atcaagcgag
     3961 ggttgaaatc attgtttaga aaaaaatcta gacagtaaga aaaagagtcg gttaactaaa
     4021 attagttgat tggctctttt aatttaaaat atataaaaaa cgaaggaaaa atccccttcg
     4081 ttttcattat attatttttt actctgcttc aattaaacca aaacgaacaa aagcattata
     4141 caaaccgtct tggtcgacat catcagttat ataatcagcc atagctttga tttcatcgcc
     4201 accacttccc atagcaacac caatatggca ataatcaagc attggaatat ctattttagc
     4261 atcaccaaag gcatatgtgt cctcaatatc tgcacctaga tactctaata aagcatcaat
     4321 agcatgagct ttagtaatat ctttaacgcc taaatcacca aatagagcaa tttcccctgc
     4381 accgccccat gtaccagctt ttaaatcagg aaattcttca attgaatcaa gatgatcttg
     4441 ataactttca agaataaaac taactttatt caaatcatca cgatataatt caccatcaaa
     4501 aatcatttct ggaaaaacat ctcgaacttt aatttgatca ctatgttctt tacctttacg
     4561 tttactatat tcttgaatta ctggttctcc ccgagtttca aagttttcac tcgcaaataa
     4621 accattatta ctttcaagat agaattcaag tcctcgactt tttaaccagt ctacgatatg
     4681 tttacattgc tcagcagtaa ttaactgatg cataacaacc gtatccccat cttctacata
     4741 actgccattc ccaccaatca tgccatcaag accaatatcc cataaataag ggtatacctc
     4801 agctttactt cgtccagtac atatatacac acgatgtcca tttttacgtg cttgtttaat
     4861 tgctttgtct gctgaagctg gcaatttgtt ttcataatca actaaagttc catcaacatc
     4921 aataaagata atttttgaat ctgccatttt tattttcctc ttttctatat gatatgacta
     4981 tatctttatt ataaaagata tagtcatatt tacaattcct caaagagaag aagaaactat
     5041 tttatctata aattatagaa attagctaaa tgaaaagata agtttctatc attttaattg
     5101 taaaagtaaa aataattttt ataaatacca aaagtataaa taaactataa taaaataaga
     5161 agtgataata acacacttct tactaatagt aatgaattta aagggtaact taaccttaaa
     5221 ttattttata tttgttgcca ttggtttaat tgctttatat gccaggaggg taacgaccgc
     5281 cccaataagt gcctcaacga taccattagt agtcacaaca ccaaataaca ttgccagcaa
     5341 tgaactatag gcaatattaa ctgcatcagc ataagcagtg ccaaagaaaa agtaaattcc
     5401 gcctaggaca agtacagtat ttgtaatcgt tccaattaaa gcaccaatca ctgctgcagc
     5461 attttcatta ttaaacttgt tttttataaa ttcaaagatt actccagcaa gatagccaag
     5521 taatattctt ggaacaagag caattataac gcttcgccaa tccccgctaa tgtttcccac
     5581 tgtaacgaat ggtgaaaaac aaaatgatgt aattcccggc tgaatcgttg cacgaattaa
     5641 actagttagt ccaaagacca aaccaagttg ggctcctgcc ttttttccta atgtaatccc
     5701 agcaatgatt acaggaatgt gcattgtcgt agcacttaaa ggaccgattg ggatataacc
     5761 aagcggtgtt acgacaagga tgatttcaat agcaagaaac attgccatga aagtcatgta
     5821 ctgtgttttt ttagatctca ttttttcctc ctactatcac tctttttaga tgtgatgttt
     5881 attatgagtg tctcgccata tcttcccatt tttctaagag tcatgatgat ctcttgaaaa
     5941 tatcttcttg atacggtttg acgataagat tatagtacaa tttattattg ctgccaactg
     6001 taataataat tatttatgta attctttgta tgattttaaa ccgtaggcgc tgtcggcttt
     6061 ttttaccgca cgattgactg ctttagccat taaatcagtt gcaagaatcc caatggcatc
     6121 tggcattact tcaacttcat tagtacttaa tacaaaaata gtatcgccat cactcatcgt
     6181 gtggacaggc tgaatagcac gggcataacc gttgtgggca atccctgcaa tctttgtaca
     6241 ttgagctttg tttaatttgg catttgtaat aatacaacca atggtagtat taccacttgg
     6301 taattggcga agtttttcaa tttgttgaaa aatcacctcg tctgcaggaa tgatactgtt
     6361 gtgttctgaa tcataaattc cagctaactg ttcattagtt ttatagtcaa caatatttcc
     6421 acaggcatta actgctacaa ttgctccaac ttgaagatta tcgatctgga tggctgcagt
     6481 acctaagccg cctttcattg catattgtgg acccatgatt ttaccaacac tagcaccaca
     6541 tccggcacca tagttaccct ctttaagcgg ttcatttttt tcgctattaa ggcaagcttg
     6601 atatcccatt gctttatctg gacgaatttt aggatcacca actgttaaat caaataaaga
     6661 tgcaccacaa acaataggga catgggcgac cttcatatca aaaccacagt tatgttcttc
     6721 taaatattgc attacaccac tagccgcatc tagtccaaag gcactgccgc cacttagcat
     6781 tacagcattg atctgttgga ccatgttgat tggatttagc aggtctgttt ctctagttgc
     6841 tgggccgcca cctcttacat caacaccagc tgtagcacca tctttgcaga tgattacagt
     6901 gcagccggtt ccagcttttt gattttcgac ttggccgatt tgaatgcctt taatttctgt
     6961 tatattaatt tttttcatgt tatcacccca atttattgta caacataatt actattattt
     7021 ccttatattt tgaagatgac aataatagtt gctaaaaatg taaattatag tttctcgaaa
     7081 atttttaaat atatagtaaa atgatactaa aataaaggag gatggaaaat gattggagat
     7141 aa
//
LOCUS       DAOMTA010000042         7127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_144_length_7127_cov_2.490950, whole genome shotgun sequence.
ACCESSION   DAOMTA010000042 DAOMTA010000000
VERSION     DAOMTA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144_length_7127_cov_2.490950"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>684
                     /locus_tag="PLK68_02035"
     CDS             212..>684
                     /locus_tag="PLK68_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 5"
                     /protein_id="HRM89219.1"
                     /translation="MKRVIREIKKAGSIAIIIAMLLSFVPTGIFALNESNYKEPLVFA
                     DFEGNDSNVDGSNNVAVSFIEGFATGTGKLVAKLELANSGDPSISERSLVITKETSVD
                     VSNYKYLTFWIKDNGTNSAKVHLIDASGNATSGNWTGNVTAGKWSQLSVSLDQFK"
     assembly_gap    685..694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <695..>3221
                     /locus_tag="PLK68_02040"
     CDS             <695..>3221
                     /locus_tag="PLK68_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HRM89220.1"
                     /translation="RNFANKVWNASRFIQMNLPEENIDLSKITGVVIGEWNKGSYLID
                     DVQFTDVLAKDLKLSASKNTGTYNDSFEVTLTAGEGQSIYYTVDGSQPTISSTKYSNS
                     LTIDESMTLKAIVVDNQNISEVYEFDYIIDHQDNRIYTPVVVQTFEDQNNFSAANGAS
                     GTIVTNEKHSGEQSLKYVKTKSEAASTSKGSIKIDFNHAVNAADLKYLIFYIKDTQGS
                     NTLQVSLIDTDGKESDFGWRSPSTTKDKWVQYCIKVSDFNKIDKTKIAGIRIGEWNAG
                     TYYLDDIYFDNYLYSGLPSLVPTKPEANISDGYIFKDRLAVTLKNDNNAPMYYTMDGS
                     TPNVDSTKYSGEIELSKTTTLKTVSFDNGKYSEVVELTYIKDTNIPSDVKADKVAGKY
                     TKPIKVTLSNEDNLAIYYTIDGSTPSKTSSKYTTPISIGETTVLKAVTYQGDSAGNVM
                     TFKYQYPTVPSEVTASIPETKFTSSKTVELISDIDANIYYTTDGSVPSLTSSRYDQPL
                     TISKSMTVKAIAERDGKTSAVTTLDYIIAPVAVQADKPAGTYDGSVVVEFRVPNNDQV
                     EIYYTTDGSVPTVASNHYTQPLRVSENTTFTVGATYKNSNDIGVVTNHTYIINPITEA
                     KAPVITPGSGTYGQRQLVSMSSDTQDSKIYYTVDGSIPSRDSMEFKKPFYVKQDTVVK
                     AITVTKNGISEITVNEIKVNQEASNFLKTDGKVIRNNYGAGEKIQLKGTNVGGWLVME
                     EWQCPTSAPDQKTMLETFTKRFGEAKAWGLINTYQDNWFTEADFITLKEEGVNCLRLP
                     ITYFEMANLDGTLKETAFDRLDWFIEEAAKHGIYTLIDMHGAFG"
     assembly_gap    3222..3241
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3242..3546
                     /locus_tag="PLK68_02045"
     CDS             <3242..3546
                     /locus_tag="PLK68_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRM89221.1"
                     /translation="PDQGDFFGKEENIQKTIKLWEAIAARYNGNEWVAGYDLLNEPGG
                     ALGTEQFEVYDRIYKAVRAIDQDHIIQIQAIWEPTHLPAPTLYGWENVVYQYHFYG"
     gene            3515..4291
                     /locus_tag="PLK68_02050"
     CDS             3515..4291
                     /locus_tag="PLK68_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89222.1"
                     /translation="MLFINIISMDNSKIKYVNEDTNYNVPVFVGEFTFFTNMDSWEYG
                     LSVFDEQGWSYTSWTYKVAGANSSWGMYTMPKNDSTNVNINTDDFETVKAKWSNFDFT
                     RNTSIADVLSKHFKIVSSDLIAPVIEGNDAAVMVGVKATVSEILDLFIKDDQDGVIDI
                     AKADITTDFDCSKAGVYTVTVEASDKAGNISEAAFTITVKEETVIDPDVVEKPDSSKS
                     EVSVNKPVKTGDNENIIGDLMILGLSMIAGVILLKRRKEI"
     gene            4400..6181
                     /locus_tag="PLK68_02055"
     CDS             4400..6181
                     /locus_tag="PLK68_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRM89223.1"
                     /translation="MRILKRISLLLLSLVMIISLSGCTADTNNKDERASFDRFIERQF
                     IETMESDYTTAHVFLEHPENFDVDISKLTVNLGVRLDEESMRNQEKKDADSWAEFKKF
                     ERSKLTKEQQDTYDIYEFQNNLAREMSDNKFDYYQQLFESISGIHYQIPTMLADWSLR
                     NEQDVKDIISLVNDVLPYLQGAIDYTKEQAKRDLLMIDVDAVREYCDNVIKSGENSLI
                     LSDINKNIDTLNLEESVGNAYKEQLKIAFNSSFIPAYQAVKDLMDEVSLTGNNEEGLV
                     KFKNGKEYYELLLQNAVGSDKSVMEIKELMEDSLDKHISKLQENVIKNASVREFLTSG
                     GEIPTTKYTSYDEILDDISAQLFEDFPSVSSLSYNIRDINEEIASSSGVAAYFNIPPL
                     DGNGIKQLRVNPSTGEVSDLDTFSTVAHEGYPGHMYQYAYMYENIQSPYQKALANSSA
                     YTEGYAVYAQFYAYKYLTGIDKNVLEALKENELANYDIMILCDIGIHYEGWLLDDFSD
                     FISEKGINLEEDSLKTQYKQLQANPAAFEPYYVGYEEFMLLKETAQNKLGDKFNDKKF
                     HEAILKSGNAPFTVVERNVAAYIKSAK"
     gene            6280..>7127
                     /locus_tag="PLK68_02060"
     CDS             6280..>7127
                     /locus_tag="PLK68_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300684.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM89224.1"
                     /translation="MYGKLAWEKYNDEQINDIMTFNEGYKNYITKGKTERLCVSETVK
                     LAMAHGYKELNEVDILKPGDKVYVTNMKKNIALFVIGKKPLEDGMRILGAHIDSPRMD
                     LKQNPLYESEGFAMLDTHYYGGVKKYQWVTIPLSMVGVVVKKDGTVINVNIGEDENDP
                     VVGISDLLVHLSADQLKKDGAKVIEGEDLDVTFGSIPLKDHEKDAVKANVLKILKDKY
                     DFDEEDFLSAEIEIVPSGKARDYGIDRSMVAGYGHDDRVCAYTSLMAILDMEMPDYTS
                     CCILVD"
BASE COUNT         2631 a          838 c         1429 g         2199 t
ORIGIN      
        1 aaaaaacgta aaacatatta atagacgata aaaataaata gttatagtca ttgattcata
       61 ataagaagtc taattttatt aaagttaggc ttcttttata ttgcagttaa atattagcag
      121 tgcacaaaga aagcctttac atttataatg atatagtata aactttattt ataccaatga
      181 attataatca ttctattaaa tggagggaac tatgaagaga gtaattagag aaattaaaaa
      241 agctggttcg attgcaatta ttatagcaat gctgttaagt ttcgtaccga caggaatttt
      301 tgccttaaat gagtccaatt ataaagaacc attggtattt gcggattttg aaggtaatga
      361 ttcaaatgtt gacggatcaa acaatgtggc ggttagtttt attgagggat ttgcaacagg
      421 aacgggaaaa ttagtagcta aactagagct tgccaatagt ggtgatccta gtattagtga
      481 acgtagtttg gtaataacaa aagagacatc agtcgatgtt tcaaactaca agtatttaac
      541 tttttggatc aaagacaatg gaacgaatag tgctaaagtc catttaattg atgccagtgg
      601 taatgctact agtggcaact ggacagggaa tgtaacggcg ggtaaatgga gtcaattatc
      661 tgtatcttta gatcaattta aaaannnnnn nnnncaagaa actttgctaa caaagtatgg
      721 aatgcttcaa gatttattca gatgaatctt cctgaagaaa atattgattt aagtaagatt
      781 actggtgttg ttatcggtga atggaataaa ggtagttatt taattgatga tgttcaattt
      841 acagatgtct tggctaaaga tttaaaatta agtgcaagta aaaacacagg tacatataat
      901 gatagttttg aagttaccct tacggctgga gaaggacaat caatctatta tacagttgat
      961 ggaagtcagc caactattag cagtactaaa tatagcaata gtcttacaat cgatgagtct
     1021 atgactttaa aggcaattgt agtcgataat caaaatatta gtgaagttta tgaatttgat
     1081 tatattattg atcatcaaga taatcggatt tatacacctg tagttgttca aacatttgaa
     1141 gaccaaaata attttagtgc agctaatggg gcatcgggaa caattgttac aaatgaaaaa
     1201 catagtggtg aacaaagtct aaaatatgta aaaactaaga gtgaagccgc tagtactagt
     1261 aaaggaagta taaaaattga ttttaatcat gctgttaatg cggcagattt aaaatattta
     1321 attttctata tcaaagatac ccaaggaagt aacacccttc aagtatcatt gattgatact
     1381 gatggaaagg agtcagattt tggatggcga agtccaagta caacaaagga taaatgggtc
     1441 caatattgta tcaaagtatc tgattttaat aagatcgata aaactaaaat agcgggaatt
     1501 agaattggtg aatggaacgc aggaacatat tatctagatg atatttattt tgataattat
     1561 ttatattcgg gattgccaag tttagtaccg actaaaccgg aagccaatat aagcgatggt
     1621 tatattttta aagataggtt agcagtaact ttaaaaaatg ataacaatgc accaatgtat
     1681 tatacaatgg atggtagtac cccaaatgtt gatagtacta aatatagcgg tgaaatagaa
     1741 ttatctaaaa caacaacact caaaacagtt agttttgata atggaaaata tagtgaagta
     1801 gtggaattaa catatataaa agatacaaat atccctagtg atgttaaagc tgataaagta
     1861 gcaggaaagt atacaaaacc aattaaagta acattaagta atgaagataa tcttgctatt
     1921 tattatacga ttgatgggtc gactccttca aaaacaagtt ctaaatatac aacgccaatt
     1981 tctataggtg aaactaccgt gcttaaggcg gtaacttatc aaggtgatag tgctggaaat
     2041 gtaatgacat ttaaatatca atacccaaca gtgccatccg aagttacagc gagcattccg
     2101 gaaactaaat ttactagtag taagacggtt gaattaatta gtgatattga tgcgaatatt
     2161 tattatacaa cagatggcag tgttccaagt ctgacttcat cacgctatga tcagcctctt
     2221 acaattagta aatcaatgac agttaaagca attgctgaac gagatggtaa aacaagtgca
     2281 gtaacaacat tagattatat tattgcgcca gtagctgttc aagctgacaa accagcggga
     2341 acttatgatg gttcagtagt ggtggaattt agagttccaa ataatgatca agtagaaatt
     2401 tattatacaa cagatggcag cgttccaaca gtggcatcaa atcattatac tcaaccgcta
     2461 cgggttagtg aaaatacaac ttttactgtt ggagcaacat ataaaaatag taatgatatt
     2521 ggtgtggtta caaatcacac atatataatt aatccgatta ctgaagctaa ggccccagtg
     2581 attacgccag gtagtggtac ttatggtcaa aggcagttag tgtcaatgag cagcgatact
     2641 caggatagta agatttatta tacagtggat ggctcaatac cttctaggga cagtatggag
     2701 tttaagaaac cgttttatgt taaacaagat actgtggtca aagcaattac agtaacaaaa
     2761 aatggcatta gtgagataac tgtaaatgaa attaaagtta atcaagaagc ttcaaatttc
     2821 ttaaaaacag atggaaaagt aattcgtaat aattatggtg ctggagaaaa aattcaatta
     2881 aaaggaacta atgttggtgg ctggctcgtt atggaagaat ggcaatgtcc aactagcgca
     2941 ccagatcaaa aaacaatgct ggaaacattt acaaaacgtt ttggtgaagc aaaagcttgg
     3001 gggttaatta atacttatca agataattgg tttacagaag ctgattttat aactttaaaa
     3061 gaagaaggag taaattgtct tagattacca atcacttatt ttgagatggc taatttagac
     3121 ggaacgctta aagagactgc atttgatcgt ttagattggt ttatagaaga agcagctaaa
     3181 catggaatct atacattgat tgatatgcat ggtgcatttg gnnnnnnnnn nnnnnnnnnn
     3241 natcctgatc aaggtgattt ttttggaaaa gaagaaaata ttcaaaaaac aattaaattg
     3301 tgggaagcaa ttgcagctcg ttacaatgga aatgagtggg ttgctggtta tgatttatta
     3361 aatgaaccag gtggagcatt aggtactgaa cagtttgaag tgtatgatcg tatttataag
     3421 gcagtaagag caattgatca agatcatatc attcaaattc aagcaatttg ggaaccaacg
     3481 cacctaccag ctccaacatt atatggctgg gaaaatgttg tttatcaata tcatttctat
     3541 ggataatagt aaaataaaat atgtaaatga agatactaat tataatgtac cggtttttgt
     3601 tggtgagttc actttcttta caaatatgga tagttgggaa tatggtttaa gtgtttttga
     3661 tgaacaaggc tggtcatata caagttggac atataaggtt gctggagcaa atagcagctg
     3721 gggaatgtac acaatgccta aaaatgacag taccaatgtg aatattaata ctgatgattt
     3781 tgaaacagtt aaagcaaaat ggtcaaattt tgactttacg agaaatacta gtattgctga
     3841 tgtattaagt aaacatttta aaatagtaag tagtgatttg attgctccgg taatagaagg
     3901 taatgatgcg gctgttatgg ttggtgtaaa agcaacagta agtgaaatcc tcgacttatt
     3961 tattaaggat gatcaagatg gtgtgattga tattgcgaaa gcagatatta caactgattt
     4021 tgattgtagt aaagcagggg tatatacagt aacagtagaa gctagtgata aagcaggtaa
     4081 tataagtgaa gcagctttta caattacggt taaagaagaa acagttattg atcctgatgt
     4141 ggttgaaaaa ccagattcat caaaatcaga agtttcagta aataagccgg tcaaaacagg
     4201 ggataatgaa aatattattg gtgatttaat gattttagga ttatcaatga tagcaggtgt
     4261 tatcttattg aaaagaagaa aagaaattta attagaggta gtaacgagat aaattctcgt
     4321 tactattttt gatataatgc atggttatgt atttaaagaa acgttataat aaatatatgg
     4381 cacaatgagg aggcaattta tgcgaatatt aaaacgaata tcattactat tattatcatt
     4441 agtaatgata atatctttga gtggctgtac agcagatact aacaacaaag atgaacgagc
     4501 gagttttgat cgatttattg aaagacaatt tattgaaaca atggaaagtg attatacaac
     4561 tgcgcatgtt tttttagaac atcctgagaa ttttgatgtt gatatttcaa agttaacagt
     4621 aaatctaggg gtgcgattag atgaagaaag catgagaaat caggagaaga aagatgctga
     4681 ttcgtgggcg gagtttaaga agttcgagcg ttcaaaacta actaaagaac agcaggatac
     4741 ttatgatatt tatgaatttc aaaataatct agctcgtgaa atgagcgata ataaatttga
     4801 ttattatcaa caattgtttg agagtatttc tggaatacat tatcagattc ctactatgtt
     4861 agctgactgg tcgcttagaa atgaacaaga cgtaaaggat attattagtt tagtgaatga
     4921 tgtcttacca tatttacaag gagctattga ctataccaaa gaacaggcaa aacgtgattt
     4981 gctaatgata gatgttgatg cggttagaga gtattgtgat aatgtgatta aaagtggtga
     5041 aaatagttta attttatctg atataaataa aaatatagat actttaaatt tagaagagag
     5101 tgttggcaat gcatataaag agcaattaaa aatagctttt aattcttctt ttattcctgc
     5161 ttatcaagct gttaaagatt taatggatga agtaagtcta accggcaata atgaagaagg
     5221 attagtcaag tttaaaaatg gaaaagaata ttatgaactg ttattgcaaa atgccgttgg
     5281 ttctgataaa tcagtaatgg aaataaaaga attaatggaa gacagtttag acaaacatat
     5341 ttccaagctt caggagaatg tgattaaaaa tgctagtgtt agagaatttc ttactagtgg
     5401 tggtgaaata ccaactacaa agtatactag ttatgatgaa attctagatg atattagtgc
     5461 tcaactgttt gaagattttc ctagtgttag tagtttatct tataatatta gggatattaa
     5521 tgaagaaata gcatcttctt ctggcgtcgc agcatatttt aatattccgc ctttggatgg
     5581 taacggtata aaacaattac gggtcaatcc atcgactggt gaggtatcag atcttgatac
     5641 tttttcaaca gtagcccatg aaggttatcc gggacatatg tatcagtatg catatatgta
     5701 tgaaaatatt caatccccat atcaaaaggc tttagctaat tcaagtgctt atacagaagg
     5761 atatgcggta tatgcccaat tttatgcata taaatatcta acaggcattg ataaaaatgt
     5821 attagaagcg cttaaggaaa atgaattagc aaattatgat ataatgattc tttgtgatat
     5881 tggaattcat tatgagggat ggttacttga tgattttagt gactttatct ctgaaaaggg
     5941 tattaatcta gaggaggaca gtttaaaaac tcaatataaa caattgcaag ctaatccggc
     6001 tgcgtttgag ccatattatg ttggttatga agaatttatg cttttaaaag aaacagctca
     6061 aaataaattg ggtgataagt tcaatgataa gaaatttcat gaggcaattt taaagagtgg
     6121 aaatgcacct tttacagttg ttgaaagaaa tgtagcagct tatattaaga gtgctaaata
     6181 aaagatttta tatgaatcta aaaaacagtt agagtattat gattgtaagt tggaaaaaat
     6241 tggtatacta tatgtatcat aagaaaagga gatgtatata tgtacggaaa acttgcttgg
     6301 gaaaagtata atgatgaaca aattaatgat attatgactt ttaatgaagg ttataaaaat
     6361 tatattacca aaggtaaaac agaacggtta tgtgtcagtg aaactgttaa actggcaatg
     6421 gcacatggat ataaagagtt aaatgaggta gatattttaa aaccgggtga taaagtatat
     6481 gttactaata tgaaaaaaaa tattgcttta tttgttattg ggaaaaaacc gctagaagat
     6541 ggaatgcgaa tcttaggtgc tcatattgat tcaccaagaa tggatcttaa acaaaaccct
     6601 ttatatgaaa gtgaaggttt tgcaatgctt gatacacact attatggtgg ggtaaaaaaa
     6661 tatcaatggg taactattcc actgtcaatg gtaggtgttg tagtaaaaaa agatggtact
     6721 gtgataaatg taaatattgg tgaagatgaa aatgatccag tcgttggaat tagtgacctg
     6781 ttagttcatt tatctgctga tcaattgaaa aaagatggtg cgaaagtaat tgaaggagaa
     6841 gacctagatg ttacttttgg aagtattcct ttaaaggatc atgaaaaaga tgctgtaaag
     6901 gcaaatgttt taaaaattct aaaagataaa tatgattttg atgaagaaga ttttttaagt
     6961 gcggaaattg aaatcgtacc tagtgggaaa gctcgtgact atggaataga tcgtagtatg
     7021 gttgctggtt atgggcatga cgatcgtgtt tgtgcttata catcattaat ggctatttta
     7081 gatatggaaa tgcctgatta tacaagctgc tgtattttag tagataa
//
LOCUS       DAOMTA010000043         7015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_151_length_7015_cov_2.486782, whole genome shotgun sequence.
ACCESSION   DAOMTA010000043 DAOMTA010000000
VERSION     DAOMTA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151_length_7015_cov_2.486782"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLK68_02065"
     CDS             complement(<1..984)
                     /locus_tag="PLK68_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HRM89225.1"
                     /translation="MKRKLNQYIRKSLLVAVTFILVFCNAVTYVNGQQYQISAGEKIK
                     YPSWFDSSGTWSTRMYTVDGKIAYCLEASKHSPGNGTVGEGSYVDNENLQKVLYYGYG
                     GPADTLSTDGVTSVEMAYLLTHIAASYYYAGDLHGVDLEKLRGWGWAEWIEAIPNRPA
                     IPKGEIGFSKNSLKMYQKDGRQRSEEITVIGDADATLTMVLDKDMELHNLTTNQTVTG
                     TVQLSSGQKFYLSSPLTKRDNYNWSSGSLMGTTNSYYRPLVLTIDDETQTIGTMVLGN
                     GETKPTSLQVEYLPVGSLKLIKTNEDKQMLDGAVFNLKGKNNDYNQDHVVKN"
     assembly_gap    1290..1299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1300..2769)
                     /locus_tag="PLK68_02070"
     CDS             complement(<1300..2769)
                     /locus_tag="PLK68_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM89226.1"
                     /translation="MEKWWQKEVVYQIYPKSFKDSNGDGIGDLQGIIEKLDYFSDLGV
                     TSLWLCPIYASPMDDNGYDISDYYAINPMFGTMEDLDELIKKGKARGIKIIMDLVVNH
                     TSDEHPWFKAAIADPTSKYRNYYIFKHGKDGRAPTNWRSIFGGSVWQKVTEDEYYFHA
                     FSKKQPCLNWENPVLRQEIYQMINWWLDKGIAGFRVDAINFIKKDQRYQDGEPDGEDG
                     LVSCIPFARNQPGIEVFFKELKENTFAKHDCMTISEAYGVSYDKLGIYIGENGCFDSM
                     FDFNYSNFDIGDNEEWFIRKDWTVKQFRDLLFTSQVEVNKVGWVGTFLENHDQPRSLD
                     KLIVNKADHGYYSATMIASMYFFLRGVPYIYQGEELGMANCVRRSIDDFDDISSHGQY
                     QRMLEEGFSESEALALVNKRSRDNSRTPMPFDDSQYAGFSDVEPWLALNESYPVINVK
                     AQFNDPDSVYSFYKKMICLRQQSVYSNTLTFGSFIPDECE"
     gene            complement(2915..4117)
                     /gene="ilvA"
                     /locus_tag="PLK68_02075"
     CDS             complement(2915..4117)
                     /gene="ilvA"
                     /locus_tag="PLK68_02075"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRM89227.1"
                     /translation="MLKLEDFKAAKQRVDEVILETHLIHSDAFSAECGNDVYIKPENL
                     QKTGAFKIRGAYNKIVKLDDEAKKHGLIASSAGNHAQGVAYAANKLGVKATIVMPSHT
                     PLIKVEATKAHGADVVLAGEVYDDAYAKAVELQESEGYTFVHPFDDEDVMEGQGTIAL
                     EILEELPDADMILVPIGGGGLISGIAAAAKLIKPDIQIIGVEPEGAASASAAIQEDQV
                     VLLNEVNTIADGTAVQQIGSKTFKYIKEYVDDIVLVNDYELMEAFLLLVEKHKLVAEG
                     SGILALAGLKKLKTKGKKVVSLISGGNIDVLTISSMINKGLISRGRIFTFSVQLPDKP
                     GQLELVSKVLNQCNANVIGVDHNQFKNFARFSEVELRVTVETNGNAHIQMIIDEFNKL
                     GYTITKIN"
     gene            complement(4243..4422)
                     /locus_tag="PLK68_02080"
     CDS             complement(4243..4422)
                     /locus_tag="PLK68_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HRM89228.1"
                     /translation="MLPNDINMLVSFLNTKLRDDDMSLAQILEINDANEIEIMELLDV
                     NGYYFDEKINQIKIK"
     gene            complement(4422..5240)
                     /locus_tag="PLK68_02085"
     CDS             complement(4422..5240)
                     /locus_tag="PLK68_02085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89229.1"
                     /translation="MTYKLIALDLDGTLKSTDKQILPKTKAILQELAKRGVVIVLASG
                     RPTAGLYAEANELELDKTGGYLLSFNGAKVVDYKTKEIIYQKVYDAKTAHQVYERAKQ
                     YNLAVMTYTDELILTEDETDEYVCIEGDINHMPIKHIDNFKDAVNFSVNKVLLTAKPE
                     YAETILDEFKAPYGDSLSIYRSAPFFIEVMAQGIDKAASLQALIERLGIKRDEVISFG
                     DGYNDLSMIEFAKFGVAMANAVDEVKKRAAYITLSNDEEGIYECLKMLSEKGEI"
     gene            5455..5919
                     /locus_tag="PLK68_02090"
     CDS             5455..5919
                     /locus_tag="PLK68_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRM89230.1"
                     /translation="MSLQQAKNYLKQFNREQDIIEFDVSSATVELAAKALNCAPERIA
                     KTLSFKINERAILIVTAGDVKIDNRKYKETFKTKAKMLARDEVLPIIGHDIGGVCPFG
                     VNPDVTIYLDESLKRFQTVFPACGNSNSAIELTIDELSSYIKAEWIDVCKTI"
     gene            6316..>7015
                     /locus_tag="PLK68_02095"
     CDS             6316..>7015
                     /locus_tag="PLK68_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRM89231.1"
                     /translation="MCGFLVVGSSELELQVFNDALNQAAYRGPDHQHVSINNGITWGF
                     NRLSIMDLSSKGNQPFVYKDCVLVCNGEVYNYHSLKAMLETDYTFKSGSDCEVLIPLY
                     HKYGIEILCKFLDAEFAMVIYDAKKDKILAARDPMGIRPMFYGYHKEDGKICFASEAK
                     GLFPLCQEIMPFPPGHYYEDGKFICYNDLSNPKTINNDDLETIATNLRLKLEKAVEKR
                     LQSDAPIGYLLSGGL"
BASE COUNT         2280 a         1396 c          933 g         2396 t
ORIGIN      
        1 atttttaacc acatgatctt gattataatc attatttttc cccttcaaat taaataccgc
       61 cccatctagc atctgtttat cctcatttgt tttgattaat ttcaaactcc ccactggtaa
      121 atattctact tgcaaactag ttggtttggt ttctccattc cctaaaacca ttgttccaat
      181 cgtttgggtt tcatcgtcaa tcgttaaaac taaaggtcga taataactat tcgttgttcc
      241 catcaaagaa ccactcgacc agttataatt gtctcgtttg gttaatggag acgataaata
      301 gaatttttga ccactactta attgtaccgt cccagtaact gtttgatttg tcgttaaatt
      361 atgtaattcc atgtctttgt ctaaaaccat cgttaaagta gcatcagcat caccaattac
      421 cgtaatttct tcactccgct gcctaccatc tttttgatac attttcaaac tattttttga
      481 aaatccaatc tctcctttag gaattgcagg acgattaggg attgcttcga tccattctgc
      541 ccagccccag ccacgtagtt tttccaagtc aacaccatga agatctccag cataataata
      601 gcttgcggca atatgagtta acagatatgc catttcaaca ctagttacac catctgttga
      661 tagtgtatca gcaggacccc catagccata ataaagtact ttttgaagat tttcattatc
      721 tacataactt ccttctccta cagttccatt acctggtgaa tgtttgcttg cttctaaaca
      781 atatgctatt tttccatcta cagtatacat tcgtgttgac caggttccac tactgtcaaa
      841 ccagctagga tatttaattt tctcacctgc actaatttga tattgctgtc cattgacata
      901 agttacagca ttacaaaaca ccaaaataaa agtcactgca actaacagtg actttcgaat
      961 atattgattt aattttcttt tcatctttct gctcctttgt tgatttatat cactaatata
     1021 atgacactct gacaaggaga acgacaataa tatctcacat ttccctccaa caaaaaagag
     1081 atctcattga atgaaatctc ttttatcata ttttatttat tttcttcaag atacataatc
     1141 gtttgatatg gtttcaatac tttaccatca tattctttat aattatttaa aataagctct
     1201 ttagcacaaa taaatggctg ctcttgattc gaaaaattac atacattgac aataagttgt
     1261 ccttgataac ttctctggta gacaatcaan nnnnnnnnnt tcacattcat cgggaataaa
     1321 gcttccaaaa gttaaagtat tactataaac actttgttgt cttaaacaga tcattttctt
     1381 ataaaaacta taaactgaat ccggatcatt aaactgtgcc ttaacattta taactggata
     1441 actttcattt aacgctaacc acggttcaac atcagaaaac ccagcatatt gactgtcatc
     1501 aaacggcatt ggagttctag aattatcacg actgcgttta ttaactagag ccaatgcctc
     1561 actttcacta aatccttctt caagcattct ttgatattgg ccatgtgaac taatatcatc
     1621 aaaatcatca atagatcgcc gaacacaatt tgccattcct aattcttctc cttgataaat
     1681 atagggaacc cctcttaaaa agaaatacat tgaagcaatc attgtagcac tataataacc
     1741 atgatctgct ttatttacaa tcaacttatc taaagaacgc ggctgatcat gattttctag
     1801 gaaagttcct acccagccta ctttatttac ttcgacttgt gaagtaaata gtaaatctct
     1861 aaattgtttt acagtccagt ctttacgaat aaaccattcc tcattatctc caatgtcaaa
     1921 attagagtaa ttaaagtcga acatactgtc aaaacatcca ttttcaccaa tatagatacc
     1981 taatttatca tatgacaccc cataagcctc agaaattgtc atacagtcat gtttagcaaa
     2041 cgtattctcc tttaattcct taaagaaaac ttcaattcct ggctgattac gcgcaaaagg
     2101 gatacaagac actaatccat cttcaccatc aggctcacca tcttgataac gttgatcctt
     2161 tttaataaaa ttaatggcat ccaccctaaa tcctgcaatt cctttatcta accaccagtt
     2221 gatcatttga taaatttctt gccgtaatac agggttttcc caatttaagc atggttgctt
     2281 ttttgaaaaa gcatgaaaat aatattcatc ttcagtaact ttttgccata ctgagccacc
     2341 gaaaatactc cgccaattag ttggcgcacg accatctttt ccatgtttaa atatataata
     2401 attccgatac ttactcgtcg gatcagcgat tgctgcttta aaccatgggt gttcatcaga
     2461 ggtgtgattt acaactaaat ccataattat cttaattcct cgtgcttttc cttttttaat
     2521 taactcatcc aaatcttcca ttgttccaaa cataggatta attgcataat aatcactaat
     2581 atcataacca ttatcatcca ttggtgaagc ataaattgga cataaccata ggctcgttac
     2641 ccctaaatca ctaaaataat ctaatttctc aataattcct tgtaaatcac caattccatc
     2701 accattgcta tctttaaagc ttttaggata aatctgataa actacttcct tttgccacca
     2761 tttctccatt attaaatcac tctcctttta ttcatataaa taaatcatat atcttcatta
     2821 taaaagccta atcccttcaa tatttctaat tttcatatta taaaaataaa tatgataaaa
     2881 aggataagct gctgcctacc cttatgtaaa aatattaatt tattttagta attgtatacc
     2941 caagtttatt aaactcatca ataatcattt gaatgtgcgc attaccatta gtctcgactg
     3001 ttactcttaa ttctacttca gaaaaacgag caaaattttt aaactgatta tggtcaacac
     3061 caattacatt ggcattacat tgatttaaaa ccttcgatac taattccaac tgccctggtt
     3121 tatcaggtaa ttgaacagag aaagtaaaaa ttcgtcctct tgaaataagt cctttattaa
     3181 tcatagacga aatagttaac acatcaatat ttccaccact gatcaatgat acaactttct
     3241 tccctttagt ttttagcttc tttaatcctg ctaaagctaa aatcccagaa ccttcagcta
     3301 ccagcttatg tttttcaact aataacaaga atgcttccat caattcatag tcattgacca
     3361 aaacaatatc atcaacatat tctttaatat atttaaacgt tttagagcca atttgttgca
     3421 cagctgtacc atcggcaatc gtattaacct cattcaataa aactacctga tcctcttgaa
     3481 tagctgcaga agcacttgcc gctccttcag gttcaacccc aatgatttga atatcgggtt
     3541 taattaattt agcagctgca gcaatccccg aaattaagcc accgccacca attggtacta
     3601 aaatcatatc agcatctggt aattcttcaa gaatctccaa tgcgatcgtc ccttgtcctt
     3661 ccataacatc ttcatcatca aacggatgga caaaagtata cccttcactt tcttgtagtt
     3721 caactgcctt agcgtaagca tcatcataaa cctcgccagc taaaaccaca tcagcaccat
     3781 gggcctttgt tgcctcaacc ttgatcaatg gtgtatgact aggcattaca atagttgcct
     3841 taactcctag tttattagct gcataagcaa ccccttgagc atgatttccc gctgaagatg
     3901 caattaatcc atgcttttta gcttcatcat ctaatttcac gatcttatta taggcacctc
     3961 taatcttgaa agcaccagtt ttttgtaaat tttctggttt gatataaaca tcattaccac
     4021 attcagctga aaaagcatcg ctatgaatta aatgtgtctc taaaattact tcatcaactc
     4081 tttgctttgc ggctttaaaa tcttctaact tcaacatttg acatgtcccc cttaattaaa
     4141 ttacattata gcatttatca ttaaaaagta aaagattgtc tttaataata tcagatgatt
     4201 aaaaatataa tcataaaaaa agacaaatca tattgatctg ccttatttaa tttttatttg
     4261 gttaattttt tcatcaaaat aatatccatt cacatccaat aattccatga tttcaatttc
     4321 attagcatca ttaatttcta atatttgtgc caatgacata tcatcatcac gtaatttagt
     4381 atttaaaaaa ctcactagca tattaatatc atttggtaac attaaatttc acccttttca
     4441 cttaacatct ttaagcactc ataaattcct tcttcatcat ttgataaagt gatataagcc
     4501 gctctctttt ttacttcatc aactgcattg gccattgcta caccgaattt tgcaaactca
     4561 atcatactca aatcattata accatcacca aagctaatta cttcatctcg tttaattcct
     4621 aaacgctcaa ttaaagcctg caatgaagcc gctttatcaa ttccttgcgc catcacttca
     4681 atgaaaaatg gtgctgaacg ataaatactt aaagaatcac catatggtgc cttaaactca
     4741 tcaagtattg tctcagcata ttcaggcttt gccgttaata agactttatt tacagaaaaa
     4801 ttcactgcat ctttaaaatt atcaatatgt ttgattggca tatgattaat atcgccttca
     4861 atacaaacat actcatcggt ttcatcttct gtaagaatca attcatcagt ataagtcatt
     4921 accgccaaat tatactgttt cgctcgctca taaacctgat gtgccgtttt agcatcgtac
     4981 actttttgat atatgatttc ttttgtttta taatcaacaa ccttagcacc attaaatgat
     5041 agtaaatagc cacctgtttt atctaattcc agctcattag cttctgcata caatccagca
     5101 gttggacgac ctgaagccaa aacaatcact acacctcttt ttgccagctc ctgcaagatg
     5161 gcttttgtct ttggtaagat ttgtttatca gtactcttca aagtaccatc taaatctaat
     5221 gcaattaatt tatacgtcat ttttctacct ctcaatataa tcaaagttta ttaaaagata
     5281 gttattttgt caaatatatt taaagataat atttgatact agtattataa ccctaaaata
     5341 acctatttta gtctcaaatc aataataaat tagtcttatt atcatctcaa tatttttgct
     5401 tgaaaatatt aataattatg gtagaattgc tttaatttat aaggaggttg catcatgtca
     5461 ctacaacaag ctaaaaatta tttgaaacaa ttcaaccggg aacaagatat tatcgaattc
     5521 gatgtttcta gtgctactgt tgaacttgct gctaaagctt taaactgtgc gcctgaacgt
     5581 attgctaaga cattatcttt taaaatcaac gaacgcgcga ttttgattgt tactgctggt
     5641 gatgttaaaa tcgataatcg caaatacaaa gaaaccttta aaaccaaagc aaaaatgctc
     5701 gcacgcgatg aagtattgcc aatcatcggt catgatattg gaggagtatg cccttttggc
     5761 gttaatcctg atgttactat ctatcttgat gaatcactta agcgatttca aacagttttt
     5821 cctgcctgcg gcaattctaa cagtgctatt gaattaacga ttgatgaact ttctagctat
     5881 attaaagcag aatggattga tgtttgtaaa accatctgat gatggttttt tattattttg
     5941 caattttttc tatcgtattg aagctttttt attgcattta ataaaaaaat aatatatact
     6001 attttcaatt actataaagt aattaaaagc aatgacgagg agagtagtaa tctacagaag
     6061 ctttagcgaa tcagggaacg gtgcaagcct gatagtgaag tagtttatga agaacaccta
     6121 agagctgtct accgaaagca ttatgcaagt aggctagaac gttaccagta acgttactat
     6181 tggcgggtat cgagtactct atgaagtatt ttccgtacca tgtaactata aattcctttt
     6241 ctagttatac ggtactcgct tctttaggcg agtttttata ttctcgcaac aataatttta
     6301 agggggaaac aaattatgtg cggtttttta gtagtaggaa gtagtgaatt agaattacaa
     6361 gtattcaatg atgcattaaa tcaggctgct tatcgtggtc ctgatcatca acatgtaagc
     6421 attaacaacg gtataacctg gggatttaac cgtctttcaa ttatggattt gtcatcaaaa
     6481 ggtaatcagc cttttgtcta taaggattgt gttcttgtct gcaatggtga agtttataac
     6541 tatcatagct taaaagctat gttagaaacg gactatactt ttaagtcagg aagtgactgt
     6601 gaagtactga ttccgctata tcacaaatac gggatagaga tattatgtaa atttttagat
     6661 gccgaatttg caatggtaat ctatgatgcc aaaaaggata aaattttagc tgcacgtgat
     6721 ccaatgggaa ttcgccctat gttctatgga tatcataaag aagacggtaa gatctgcttt
     6781 gcttcagaag caaaaggatt atttccttta tgccaagaaa taatgccatt tccaccaggg
     6841 cattattatg aagatggcaa atttatttgt tataatgatt taagtaatcc taaaacgatc
     6901 aataacgatg atttagaaac aattgcaaca aatttacgcc tcaaattaga aaaagcagtg
     6961 gaaaaaagat tgcaaagcga tgctccaatc ggatatttat taagcggtgg tttag
//
LOCUS       DAOMTA010000044         6938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_156_length_6938_cov_2.540171, whole genome shotgun sequence.
ACCESSION   DAOMTA010000044 DAOMTA010000000
VERSION     DAOMTA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156_length_6938_cov_2.540171"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLK68_02100"
     CDS             complement(<1..950)
                     /locus_tag="PLK68_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRM89232.1"
                     /translation="MIKNFFNKLSIQISAIIIICGTLGVATFCLLYAGKESFFEFTQN
                     LGVISENTEAYANNIENQIIDQNVSITDVDTLDKILGTSDIYSVNLYNKADNLAITGS
                     FATMLDNFIIGSTVYDTEAIYNGDDYSTEIELKDGTIEMYVYSYALAKLVVPYIVSSI
                     VIALFTFLLPTFLFIRRKVNYMTTLKNEVTIMSQGDLDHTIKINSNDEIAELSNQIDN
                     LRLTLKDNFATEEANRKANYELVTALSHDLRTPLTSLMGYLDIIRLKKFKNEDQYNLY
                     LKNSIDKVNQINELANKMFEYFLVFSKDQDTELSKMSLGV"
     gene            complement(943..1644)
                     /locus_tag="PLK68_02105"
     CDS             complement(943..1644)
                     /locus_tag="PLK68_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM89233.1"
                     /translation="MTNNKILIVDDNPEIREVLNVLLSSEGYDVIEAKDGQEAIDMVS
                     DDIDLYILDIMMPIINGYQACVEIRKKSNAPILFLTAKGQESDKTLGFSSGGDDYLSK
                     PFSYNELTTRVKALLRRYYVYQGKMEKEENNDDNIVYNNITINPNSEAVYLNGEQIEL
                     TYLEYQILYLLLSNRKRIFSTQTLYESIWNEPYYYSANNTIMVHIRNLRKKIESDPQN
                     PKIIKTIWGKGYRCD"
     gene            1785..4226
                     /locus_tag="PLK68_02110"
     CDS             1785..4226
                     /locus_tag="PLK68_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM89234.1"
                     /translation="MKKKQFYPIIFLTCLTLVMIIVITPSSSIFGANTDWLSQHVNLA
                     DYIRKTMLETKSFFPDFAFNIGGGQNIYNISYYGLFRPDVLIGCLIPGIAMKDIIITY
                     MIINLVLSVNLTYIWLKRKQFSTTLCIVGAVLLLSSSVLFQSHRQIMFVDYMPGVLLA
                     LIAVDTYIKNQKQGLLIGAVVLIIANSYFFAISALLTIFTYYCFEMYRLKNLITFKKL
                     CLFIQPLLIGVLICGILLIPTAYVMLENHQSSGGSLDLMSLVVPRGDFKALLYNAYGC
                     GLGFVAWAGLVLSLKLKETRKLSIWLLLLLFIPIFSFVLNGFLYPRAKILLPFTPLII
                     YIVIQTINEYKQSQIKLDLKLIVLLILPIVLFYKEPLVILDIIICLVGILLYLRITER
                     TLYLLLVMPLIISYVNNQKESFVTKDTYQQVSNLKNIKVEKDGRYDIFKQSLNTVNQV
                     SNNELRSSIYSSVSNRLYNHFYYDIIKNPISIKNRVACLSNSNIFFQGMMGVKTLYSE
                     NVVPMGYQAIGNNLYQNDKVLPLVYATSNSYDVVQFDRLDYPQTLDTIYNNVVVAGGQ
                     SNYQSKAATVALETVVKEKSDNLQITKLNDGYRINTKKTGKLELGINQDLTNKILIVE
                     SDLAKVKLIKRKDTSITINGVKNKLSSSSAAYPNHNTHFTYVLSQNELLDQLSISFSN
                     GRYDLKNIKVSTIDYDVIKNRNQEIDALVGSYNQDGNLVEGTINVSNDGYLVTSLPYQ
                     NGYTVLIDGKEVAKECVNKAFLGAKISKGQHQIRIIFKAPMKNVGYVCSGVGFIWLVF
                     QGRRKKNEKGFERIN"
     gene            4198..4605
                     /locus_tag="PLK68_02115"
     CDS             4198..4605
                     /locus_tag="PLK68_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRM89235.1"
                     /translation="MKKDLKELISYVFVGGCTTLINFVIYWFVITMMDQGWLFANVIS
                     WVGAVIFAFWANKHFVFKSANETGKEAYQFFILRLGTLLVESGLLFIFIQLLSANEML
                     SKVVVSVITVVSNYGLCKFKIFAAKGGHEYGQN"
     gene            4592..5536
                     /locus_tag="PLK68_02120"
     CDS             4592..5536
                     /locus_tag="PLK68_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM89236.1"
                     /translation="MDKISVIVPCYNEEEVLPLFHKEVTKELETIENIDYEILFIDDG
                     SHDHTIDLLKMICAKDHHCDYYSFSRNFGKEAAMFAGLEKSTGDYCVIMDADLQHPPK
                     LLAPMYQAVSQEGYDCCAGKRMDREGEGRLRNFLSKSFYKVMQKLSKLDMSDGAGDFR
                     MMSRLMVDSILEIREYNRYMKGLFSYVGFETKWLPFNNVERAAGSTKWNFKSLFSYAL
                     EGIFSFSTAPLKIAGIFGVILFVGSIILSLYTAISTLMFGNSVGGYTTIVCLILFLSG
                     TQMLLIAILGEYVSKDYMENKARPIYIVKDTNRRKRYN"
     gene            5653..6498
                     /locus_tag="PLK68_02125"
     CDS             5653..6498
                     /locus_tag="PLK68_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89237.1"
                     /translation="MKKFGNVVLTIVMLCLIMLLCVNLSIKTMSTKAITNAVVVQEAS
                     NGIEEVLNQAFPDVSNENIKKVEEAVKNNSALNDVTSDLLDQITAAVANGSDVDTAAI
                     AAQLSKAVDENIPAIEEAIGKKITTEQREQIQSKITDENGALQNKIVSTVEKVQKTTP
                     GTQKFIKTYKTLSDTPTRIICVVGIILTAVLLGLINKSFYKWTLFSGIAAVISGIVVG
                     LFMPLVVSAMEFTIGNRLLGMSIDIPVGSLRLDGAICAGAGIILIVAYIVLNKKYATF
                     ERHYY"
     gene            6737..>6938
                     /locus_tag="PLK68_02130"
     CDS             6737..>6938
                     /locus_tag="PLK68_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM89238.1"
                     /translation="MIRIMLACAGGMSTSLLMNKMKEEADNRGLEVSVEAIGEKTLEQ
                     HLGEFDVLLLGPQVRYVIPNVKK"
BASE COUNT         2379 a          869 c         1227 g         2463 t
ORIGIN      
        1 attacgccga gtgacatctt agataattca gtatcttgat ctttactaaa gacgagaaag
       61 tattcaaaca ttttatttgc tagttcatta atttgattta ctttatcaat tgaatttttc
      121 aaatataaat tatattgatc ttcattttta aatttcttta gtctaataat atctaaatat
      181 cccattaaag aagttaaagg agttcgcaaa tcatgagaaa gtgctgtaac taattcataa
      241 ttagctttgc gattagcttc ttcagtagca aagttgtctt ttaaggttag gcgaagatta
      301 tcaatttgat tagataattc tgcaatttca tcattactat taattttgat tgtatggtct
      361 aaatcacctt gtgacataat tgtaacttca ttttttaatg ttgtcatata atttacttta
      421 cgtcgaataa ataaaaaggt tggaagtaaa aaggtaaata aggcaatgac aattgaggaa
      481 acgatgtaag gaacgactaa tttagctaaa gcatatgagt aaacatacat ttcaatggtt
      541 ccatctttta actcaatttc tgtactataa tcgtcaccgt tataaattgc ttcagtatca
      601 tatactgttg aaccgatgat aaaattgtca agcatggtag caaaagaacc agtgatggca
      661 aggttatcag ccttattata tagattgaca ctataaatat cgctagtacc taagatctta
      721 tctagagtat cgacatcagt aattgataca ttttgatcaa taatttgatt ttcaatatta
      781 tttgcataag cttcagtatt ttcagagatg acccctaagt tttgggtaaa ttcaaagaaa
      841 ctttctttac cggcataaag tagacaaaaa gttgccaccc ctaaggttcc acaaataatg
      901 ataatggcac taatttgaat acttaattta ttaaagaaat ttttaatcac aacgataacc
      961 cttaccccaa attgttttaa ttatttttgg attttgagga tcactctcta tttttttacg
     1021 aagatttcga atatgaacca taattgtgtt attagcacta taataataag gttcattcca
     1081 aatactctca tacaatgttt gcgttgaaaa aattctttta cgattactta ataaaagata
     1141 aagaatttgg tattctaagt aagttaattc aatttgttct ccgtttagat acactgcttc
     1201 actattagga ttaatagtaa tattattgta aacaatgttg tcatcattat tttcttcttt
     1261 ttccattttc ccttgataaa catagtatcg acgcaacaat gccttcactc ttgtcgttaa
     1321 ttcattataa gagaatggtt ttgataaata atcatcccca ccactagaaa aaccaagtgt
     1381 tttgtcactt tcttgacctt ttgctgttaa aaataagatt ggggcgttag atttttttct
     1441 aatttcaaca caggcttggt aaccattgat tataggcatc ataatatcga gaatatataa
     1501 atcgatatca tcactaacca tatcaattgc ttcttggcca tctttagcct caatcacatc
     1561 ataaccttca cttgagagca aaacatttaa tacttctcta atttcaggat tgtcatcgac
     1621 aattaaaatt ttattgttcg tcatatattc accaactttc gtatttatta tacatcataa
     1681 tggaaattaa gaagatagta aaatgaaaat taagaaatat ttagatgttc tttaggtgat
     1741 tcttaaggaa tatatacgat aatgtgagca ggagaatgat agtaatgaaa aaaaagcagt
     1801 tttacccaat tatattttta acatgtttaa ccttagtgat gatcatagtc atcacgcctt
     1861 cttctagtat ctttggagct aatactgatt ggctatcgca acatgtcaat ctagctgatt
     1921 atattcggaa gacgatgtta gagacaaaat catttttccc cgattttgca tttaatatcg
     1981 gcggtggtca aaatatttat aatatatctt attatggatt gtttcgacct gacgtattaa
     2041 ttggctgtct tattcctgga atagcaatga aagatattat aattacctat atgataataa
     2101 atttagtttt aagtgttaac ctgacctata tatggttgaa acgcaaacag tttagcacaa
     2161 cgctatgtat agttggagct gtattattat tatcttcttc tgtattattc caaagtcacc
     2221 gccaaatcat gtttgttgat tatatgcctg gagtgctttt ggcattgatc gcggtagata
     2281 catatattaa aaatcaaaag caagggttat taattggagc agtggttcta ataattgcaa
     2341 atagttattt ttttgcaatt tcagctttac taacaatttt tacatattat tgttttgaaa
     2401 tgtatcgatt gaaaaaccta attactttta aaaagttatg tttatttatt cagccgttat
     2461 tgatcggagt gcttatttgt ggaattcttt taattccaac tgcctatgtt atgctggaaa
     2521 accatcaaag tagtggcggc tcacttgatt tgatgtcgtt agtagtgcca cgaggtgatt
     2581 ttaaagccct attgtataat gcttatggtt gtggattagg ctttgttgca tgggcaggtt
     2641 tagttctaag cttaaaatta aaagaaacga gaaaattatc gatctggcta ttattgttat
     2701 tatttatccc gatctttagt tttgtcttaa atggtttttt atatcctcgg gccaagattt
     2761 tgcttccttt tacaccattg attatttata tagtaattca aacaatcaat gagtataaac
     2821 aatcacaaat taaattggat ttaaaattaa ttgtgctact tattttacca attgtcttat
     2881 tttataaaga gccattagtt attttagata ttataatttg tttggttggg atattattgt
     2941 atctaagaat aactgaacga actttatatt tattgttggt aatgccttta ataattagct
     3001 atgttaataa tcaaaaagag agttttgtta caaaagacac ttatcaacaa gtttcgaatt
     3061 taaaaaatat taaagtagaa aaagatggtc gatatgatat ttttaaacag tcattgaaca
     3121 ctgtcaatca agttagtaat aacgagctta gaagttcaat ctatagttcc gttagtaata
     3181 ggctctataa tcatttttat tacgatatta ttaaaaatcc tattagtatt aaaaatcgtg
     3241 tcgcttgtct atcaaattca aatattttct ttcaaggaat gatgggagtg aagacgctct
     3301 atagtgaaaa tgtagtaccg atgggatatc aagcaattgg taataatcta tatcaaaatg
     3361 ataaagtatt gccgcttgtt tatgcaacta gtaatagcta tgatgttgtg cagtttgatc
     3421 gtctagatta cccacaaaca ttagacacta tttataataa tgtggttgtt gcaggcggtc
     3481 agagtaatta tcaaagtaag gcagctacgg ttgcactaga aacagttgta aaagaaaaaa
     3541 gcgataattt acaaataact aaattaaatg atggctatcg tattaacact aaaaagacgg
     3601 gtaaattaga attagggatc aatcaagatt taactaataa gattttaatt gtggaatctg
     3661 atttagctaa agttaaatta attaagcgta aagatacgag tattacgatt aatggggtaa
     3721 aaaataagtt atctagttca agtgctgctt atccaaatca taatacgcat tttacatatg
     3781 ttttaagtca aaatgaattg cttgaccaac ttagtattag tttcagtaat ggacgttatg
     3841 atttaaagaa tataaaagtt tctacgattg actatgatgt aatcaaaaac cgtaatcaag
     3901 aaattgatgc attagttggc tcgtataatc aagatggcaa tcttgtagaa ggaacaatca
     3961 atgttagtaa tgatggctat ttagttactt cattaccgta tcaaaatggc tatacggtat
     4021 taattgatgg caaagaagtt gctaaggagt gtgtaaataa agcattttta ggagcaaaga
     4081 tttctaaagg acaacaccag attagaatta tatttaaagc tccaatgaaa aatgttggtt
     4141 atgtttgtag tggtgtgggt tttatatggt tagtatttca agggagaagg aaaaagaatg
     4201 aaaaaggatt tgaaagaatt aattagttat gtatttgtag gtggatgcac aacgctaatt
     4261 aactttgtaa tttattggtt tgtgattaca atgatggatc agggctggct ttttgcaaat
     4321 gtaatctctt gggttggtgc tgttatcttc gctttttggg ctaataaaca ttttgttttt
     4381 aaaagtgcta atgaaactgg aaaagaagct tatcaattct ttattttaag attagggaca
     4441 ttattagttg aaagcggatt attatttatt tttattcagt tattatctgc aaatgagatg
     4501 ttatcaaaag tagtagttag tgtaattaca gtagtttcaa attatgggct atgcaagttt
     4561 aagatttttg ctgccaaagg aggacatgaa tatggacaaa attagtgtaa tcgtaccttg
     4621 ttataatgaa gaggaagtac ttccattatt ccataaggaa gtgactaaag aacttgaaac
     4681 gattgaaaat attgattatg aaatcttatt tattgatgat ggtagtcatg atcatacaat
     4741 tgatttatta aagatgatct gtgctaaaga tcaccattgt gactattatt cattttctcg
     4801 caactttgga aaagaagcag cgatgttcgc tggcttggaa aaatcaaccg gtgattattg
     4861 tgtaatcatg gatgcagatc ttcaacatcc cccaaaattg ctggcaccga tgtatcaggc
     4921 agtaagtcaa gaaggttacg attgttgtgc tggaaaaaga atggatcgtg agggtgaagg
     4981 tagattaaga aatttcttaa gtaagtcatt ttataaggtg atgcaaaaat tatcaaaatt
     5041 ggatatgagt gatggtgcag gagattttag aatgatgagt cgtcttatgg ttgattcaat
     5101 tttagaaatt agagagtata atcgttatat gaaaggttta ttttcatatg ttggttttga
     5161 gacaaagtgg ttaccattta ataatgtaga aagggctgct ggttctacta aatggaattt
     5221 taaaagctta ttttcttatg ctttagaagg aattttctca ttttctactg ctccactaaa
     5281 gatagcagga atatttggtg ttatcctatt tgttggttca attattttat cgttatatac
     5341 agcgatttca acattgatgt ttggaaatag cgtaggtgga tatactacga ttgtctgttt
     5401 aattttattt ttaagtggaa cacaaatgtt attaattgca atattaggag aatatgtgtc
     5461 taaagattat atggaaaata aagctcgacc aatttatatt gttaaagata caaaccgcag
     5521 aaaacggtat aattagagtg tgatttggca ctctttttga cttaagagat aaaaaaaact
     5581 ataataaata cattagaaaa gatatcttat aatttagaaa ttggaacaag gcatcttatt
     5641 gaggagaaag aaatgaagaa atttggaaat gtagtattaa caattgtcat gttatgtttg
     5701 attatgctcc tatgtgttaa tctatcaatt aagacaatgt caactaaagc aattacaaat
     5761 gctgtcgttg ttcaagaggc aagtaacggg attgaagaag tacttaatca agcatttcct
     5821 gatgtaagta atgaaaatat caagaaagtt gaggaagcag taaaaaataa tagtgcttta
     5881 aatgatgtga cgagcgattt attagatcaa attacagcag ctgtagcaaa tggtagtgat
     5941 gttgatactg ctgcgattgc tgcacaatta tctaaagctg ttgatgaaaa tattcctgca
     6001 atcgaagaag caattggtaa aaaaatcact acagagcagc gtgaacaaat tcaaagtaag
     6061 attactgatg aaaatggtgc cttacaaaat aaaattgtaa gtaccgttga aaaggtccaa
     6121 aaaacaaccc caggaacaca aaaatttatt aaaacttata aaactttaag tgatactcca
     6181 acgagaatta tttgtgtggt gggaattatt ttaacagcag ttttacttgg tttgattaat
     6241 aaatcatttt ataaatggac tttatttagt ggaatcgctg ctgttatttc aggtattgta
     6301 gttggtttgt ttatgccatt agttgttagt gcaatggagt ttactattgg aaatcgtcta
     6361 ttaggaatga gcattgatat tccggtaggg agtttacgtt tagatggtgc tatttgtgct
     6421 ggtgctggaa ttatcttgat tgttgcatat atcgttttaa ataagaaata tgctacattt
     6481 gaacgtcatt attattaaga tatagattag gaattcaggg gaaggtagca gtatcttctc
     6541 ttttttatgt aatttatttt ggtttgatat taagatataa ctattagaac tggtagttat
     6601 tcttataatg atgaaataaa tttctcataa tttttatgta aatagggcaa tatattaaaa
     6661 gaaaaattcg ttttaataga aaacgatttt tttatatcca aatagttttt acaatggagt
     6721 caaggaggaa aagaaaatga ttagaattat gttagcatgt gctggaggga tgtctaccag
     6781 cttattaatg aacaagatga aagaggaagc cgataataga ggactggaag tcagtgttga
     6841 agcgattggt gaaaaaacat tggaacagca tttaggcgaa tttgatgtat tactgttagg
     6901 accgcaagtt cgttatgtta ttcctaatgt taagaaaa
//
LOCUS       DAOMTA010000045         6827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_162_length_6827_cov_2.836533, whole genome shotgun sequence.
ACCESSION   DAOMTA010000045 DAOMTA010000000
VERSION     DAOMTA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_6827_cov_2.836533"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /gene="rpmH"
                     /locus_tag="PLK68_02135"
     CDS             <1..105
                     /gene="rpmH"
                     /locus_tag="PLK68_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRM89239.1"
                     /translation="KRSKTHGFRARMATVGGRKVLARRRKRGRKVLSA"
     gene            146..484
                     /gene="rnpA"
                     /locus_tag="PLK68_02140"
     CDS             146..484
                     /gene="rnpA"
                     /locus_tag="PLK68_02140"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRM89240.1"
                     /translation="MKKEFRVRKNEDFSRIIKKKQSMANRSFIIYYLKNDLDHARIGI
                     SVSKKLGKAVIRNKIKRQVRMMLQQTINFDDNYDYIVIIRNKYLDLDFNSNLNELKYL
                     YKKILKRMEK"
     gene            485..1381
                     /gene="yidC"
                     /locus_tag="PLK68_02145"
     CDS             485..1381
                     /gene="yidC"
                     /locus_tag="PLK68_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRM89241.1"
                     /translation="MVLDNKTKKILKVFCLVFLVAILTGCAQNLDANGKLIASRAITS
                     STPWSFDAGWFDFIFVIPLAKGILFINDYVGNVAFGVIGVTIIVNIITLPIMIKSTVS
                     TQKMQLLQPEMEKIQRKYKGRKDQASQMRQNAEIQNLYKKNNVSMFGSFATFLTLPIM
                     FAMWQAVQRIDILYSTYIFGINLGAKPISHIMDLDIAYIVLLVLVAGTQFFAMQITQI
                     MAKRSPKYRPSQQMKSMNTMNNVMTIFIVYLAATMPAAMSLYWITTNVINIIRTVYIQ
                     LFHIEKAKKEVESTTTNFLNKK"
     gene            1390..1989
                     /gene="jag"
                     /locus_tag="PLK68_02150"
     CDS             1390..1989
                     /gene="jag"
                     /locus_tag="PLK68_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRM89242.1"
                     /translation="MKRYTARTVQDAVNTACQELGVTIDELNYEVISETKTLFTKKAE
                     IECYTIPMIQEYIESYIRRFIGDMGFEVETVSYLQDGRIYCNINTSNNSILIGKAGVI
                     LRAINFIVKNAVSNTFKKRFEISVDINGYKEDRYKKVASMAKRFGKQVLRTKAEIKLD
                     PMPADERKVMHQELSRFEHIKTESHGEGKNRHMVISYVD"
     gene            2033..2554
                     /locus_tag="PLK68_02155"
     CDS             2033..2554
                     /locus_tag="PLK68_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM89243.1"
                     /translation="MNIELARATDVNEIEALYGAVVDDLLANVNYPCWKKGIYPTRDE
                     AIFGIEKKELYVMRQNDQIVGSIVINHVQEENYQLAAWKIDAQDHEVYVIHTLAVHPQ
                     FKGLKIAQKLLEYADELAKNNGVKTIRLDVRKGNVPAIKLYERCGYTYVGAINLDFRG
                     SDLGLFELYEKII"
     gene            2613..2918
                     /locus_tag="PLK68_02160"
     CDS             2613..2918
                     /locus_tag="PLK68_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89244.1"
                     /translation="MDKIVINQELLLLLQKNKIIATTSNFNYRDQAVISLIKTLITMG
                     YNSSDILELIDEEMTLLDILEFHQDLVINKQYECCCLINKLKKMIEEVKEETNGIYN"
     gene            2902..3264
                     /locus_tag="PLK68_02165"
     CDS             2902..3264
                     /locus_tag="PLK68_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89245.1"
                     /translation="MEYTIKEVTKKYNLSASTLRYYEKEGLLPKIKKNQSNQRVYDDD
                     DLSWLDIIMCMRKTGMTIAYIKNYIELCQEGDNTINQRYEIFLKQKEILLLQQQELEK
                     NIETVNYKINLYKEKLLK"
     gene            3330..4520
                     /locus_tag="PLK68_02170"
     CDS             3330..4520
                     /locus_tag="PLK68_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM89246.1"
                     /translation="MNKNKLLMFFIINGLFNLAANYAHPITPTVIKNLQLGDYMFGVA
                     FGAMMGLNFLFSPFWGKLNEQINSKTTMLICSIGYAVGQVLFWQANSQNSVIFARMFS
                     GVFTGGSYVSFLTYVVNVTPPEKRGQNLTVLATVASVCAAFGYFIGGMLGEISIDLTF
                     AVQAATLASCGIFFYLFCENDVVSPVKQKVALLIKQANPFNSFKQAKVFIDKKYLIMF
                     TICCLAFLGFTAFEQSFNYYIKDIYNLTSGYNGIIKAAIGIISLVANGTICLWIIKNT
                     NVSRSSMLILLLSSVTMLGVLATNNIIIFIVMNIIFFALNAIIIPVTQDLVASRSNDT
                     NSSIVMGFYNAIKSLGGIIGALMAGFFYTFNPRLPFIFGFVAFLLATVLSYYYYQQNK
                     LHKS"
     gene            complement(4766..5950)
                     /gene="rho"
                     /locus_tag="PLK68_02175"
     CDS             complement(4766..5950)
                     /gene="rho"
                     /locus_tag="PLK68_02175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRM89247.1"
                     /translation="MESIKNDKNKVNTKEEDELGELASGILEIMVDGYGFLRPKNYTQ
                     ESRDIYISQSQIRRFNLKNGDEIVGHARNSKNGERYNALMRVDLVNGQNPDQAKKRIA
                     FEFLTPIYPNERVYLGKDNEQLSMRLIDLISPIGKGQRGIIVAPPKAGKTTLLKDIAL
                     SVSKNYPNIKIFILLIDERPEEVTDIKEALDADNVEVIYSTFDEKSESHIKVAQTTLE
                     RAKRLTEQGYDTMILLDSLTRLTRANNLTVTPSGRTLSGGLDPASFYFPKRLFGAARN
                     TREGGSLTILATALIETGSRMDDMVYEEFKGTGNMELVLSRKLQEKRIFPAIDVQKSG
                     TRREDLLLSKDEYEAMQIIRKSLDQTTIEQATDSLLNMFEKTKDNEVLIKNILVSTKH
                     QK"
     gene            6126..>6827
                     /locus_tag="PLK68_02180"
     CDS             6126..>6827
                     /locus_tag="PLK68_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM89248.1"
                     /translation="MDIMVVINQMIQLFLIIGLGYFMQKKKILNDELNTKLNFIVISI
                     TTPALIFSSVCTTSISEKSMVIYTLAIATAVYVALPVISFVLVKLMRIPMHQKGLYMF
                     MTIFSNTGFMGYPIMKALFGNDAVFYTALFNILFNLEVFTLGVILINYGNDVKMKLNP
                     KNLLSPGVVASIIAIFIYFLEIPIPDVIANSCGMVGDMTTPLAMMIIGATLANIKVKE
                     LFTELRLYYFTIVKQV"
BASE COUNT         2406 a          869 c         1127 g         2425 t
ORIGIN      
        1 aaaagatcaa aaactcatgg ttttagagct agaatggcta ctgtaggagg aagaaaagta
       61 cttgcacgtc gccgtaaaag aggaagaaaa gtcttatctg cttaagaaaa atccacttta
      121 cggtgggttt tttttctaaa aatttatgaa aaaagaattt agagtacgaa aaaatgaaga
      181 ttttagccgt attattaaga aaaaacaaag tatggctaat cgttctttca ttatatatta
      241 cctaaaaaat gatttagatc atgctcgaat tgggatatca gtaagcaaaa aacttggaaa
      301 agcagtaatt cgtaacaaaa ttaagcgtca agtcagaatg atgcttcaac aaacaattaa
      361 tttcgatgat aattatgatt atatagttat tattagaaat aaatatttag atttagattt
      421 caattctaat ctaaatgagc taaaatattt atataaaaaa atattaaaaa ggatggaaaa
      481 ataaatggtg ttagataaca agactaaaaa aatcttaaaa gtattttgtt tagttttctt
      541 agttgctatc ttgacaggat gtgcacaaaa tcttgatgca aacggtaaat taattgcttc
      601 tagggcaatt acaagttcaa ctccttggtc atttgatgca gggtggtttg attttatctt
      661 tgtaattcct ttagctaaag gaatcttatt cattaatgat tatgtaggaa atgtagcttt
      721 tggggtaatt ggggttacaa ttatcgttaa tattatcact ttacctatta tgatcaaatc
      781 aaccgtctca acgcaaaaaa tgcaattatt acaaccagag atggaaaaga ttcaacgtaa
      841 gtataaaggg cgtaaagatc aagcttcaca aatgcgtcaa aatgcagaga ttcaaaattt
      901 atataaaaag aataatgtaa gtatgtttgg atcatttgca acattcctta cattaccaat
      961 catgtttgca atgtggcaag cagtacaaag aattgatata ttatattcta catatatttt
     1021 tggaattaat ttaggggcta agccaatcag ccatattatg gatttggata ttgcgtatat
     1081 tgttttattg gttttagttg caggaacaca attctttgca atgcaaatta ctcaaataat
     1141 ggcaaaacgc tcaccaaaat atcgtcctag tcaacaaatg aagtcgatga atacaatgaa
     1201 caatgtaatg acgatcttca ttgtttactt agctgctaca atgcctgcag caatgtcgtt
     1261 gtactggatt acaacaaatg ttattaatat cataagaaca gtatatattc aattattcca
     1321 tattgagaaa gcaaaaaagg aagttgaatc aacaactact aattttctaa ataaaaagta
     1381 ggtgaaaata tgaaaagata tacggcaaga acagttcaag atgctgtaaa tacagcatgt
     1441 caagaactag gtgtaacaat tgatgaatta aattatgaag taattagtga gacaaaaact
     1501 ttatttacta aaaaagctga aattgagtgt tatacaattc caatgatcca agaatatatt
     1561 gaaagttata ttcgtcgttt tattggagat atgggatttg aagtggaaac agtttcttat
     1621 ctacaagatg gtcgtattta ctgtaatatt aatactagta ataattctat cttaattgga
     1681 aaagctggag ttattctacg tgcaattaac tttattgtta aaaatgccgt aagcaatacc
     1741 tttaagaaaa gatttgaaat tagcgttgat attaacggat ataaagaaga ccgttataaa
     1801 aaagtagcat caatggctaa acgttttgga aaacaagtat tgcgcacgaa agctgaaatc
     1861 aagcttgatc caatgcctgc tgatgaacgt aaagtaatgc atcaagaact ttctagattt
     1921 gaacatatta agacagaaag tcatggtgaa ggaaaaaata gacatatggt tatttcatat
     1981 gtagattaga cacatgttat catgtgtttt ttctttaagg agtgatatga agatgaatat
     2041 agaattagca agggcaactg atgtaaatga gattgaagcg ttatatggag cagttgtaga
     2101 cgatttacta gccaatgtaa attatccttg ctggaaaaaa ggaatttatc caactcgtga
     2161 tgaagcaatt tttggaatag aaaaaaaaga attatatgta atgcggcaaa atgatcaaat
     2221 agtggggagt attgttatta atcatgtcca agaagaaaat tatcaattag cagcttggaa
     2281 gatagatgca caagatcatg aagtttatgt aattcatact ttagctgttc atccacaatt
     2341 taaaggatta aaaatagctc aaaaattatt agaatatgct gatgaattag ctaaaaataa
     2401 tggggtaaaa acaattcgtt tagatgttcg taaaggcaat gtgccggcaa tcaagctata
     2461 tgagcgttgt ggttatacat atgtgggagc aataaatctt gattttaggg gttctgatct
     2521 tggcttattt gaattgtatg aaaaaataat ttaaataatg cttgccctat agttaggttt
     2581 aagtagtatg cttaacttag gaggacgact tcatggataa aatagttatt aatcaagaac
     2641 ttttattgct acttcaaaaa aataaaataa tcgccacaac gtcaaacttt aattaccggg
     2701 atcaagcagt catatcacta atcaaaacat tgattactat gggatataat agtagtgata
     2761 tcttagaatt gattgatgaa gaaatgactt tgctggatat tctagaattt catcaagatt
     2821 tggtaattaa taaacaatat gaatgctgct gtttaattaa taaattaaag aaaatgattg
     2881 aagaagttaa ggaagagacc aatggaatat acaattaaag aagtaactaa aaagtacaat
     2941 ctaagtgctt caacacttag atattatgaa aaagaaggat tactacctaa aataaaaaag
     3001 aatcaaagca atcaaagagt ttatgatgat gatgatctat cgtggttaga cataattatg
     3061 tgtatgcgta aaacgggaat gacgatagca tatataaaaa attatattga gttgtgtcaa
     3121 gaaggtgaca atacgattaa tcaacgatat gaaatttttt tgaaacagaa agaaatatta
     3181 ttattgcagc aacaagagtt ggaaaagaat attgagacag tgaattataa aattaatcta
     3241 tataaagaaa agttattaaa atagatagca agaattattg ctatctattt ttctttcaat
     3301 tataatcaat ataataagta ggtgataaga tgaataaaaa taaattatta atgttcttta
     3361 ttattaatgg actttttaat ttagccgcta attatgctca tccaattact ccaacagtaa
     3421 taaaaaatct tcaactggga gattatatgt ttggtgttgc atttggggca atgatgggat
     3481 taaatttttt attttcacca ttttggggaa aattaaatga acaaattaac agtaaaacaa
     3541 cgatgttgat ctgtagtatt ggttacgcag ttggtcaagt tttgttttgg caagcaaatt
     3601 cacaaaatag tgtgatattt gcaagaatgt tttcaggcgt ttttacagga ggttcttatg
     3661 taagtttttt aacctatgtc gttaatgtta caccacctga aaaaaggggt caaaatctaa
     3721 ctgttttagc aacagttgca agtgtttgtg ctgcttttgg ttattttata ggggggatgc
     3781 ttggggaaat ctcaattgat ttaacttttg cagttcaagc agctacttta gctagctgtg
     3841 gtattttctt ttatcttttt tgtgaaaatg atgttgtttc accagtcaaa caaaaagttg
     3901 ctctgctcat aaaacaagca aaccctttta attcatttaa acaagcaaaa gtatttatag
     3961 ataaaaaata tttaattatg tttacgatct gctgccttgc atttcttggt tttactgctt
     4021 ttgaacaaag ttttaattat tatatcaaag atatttataa tttaacatca ggttataatg
     4081 gaattatcaa ggctgcaatt ggaattattt ctttagttgc taatggtacg atttgtttat
     4141 ggatcattaa gaatactaat gtttctagat cgtcaatgct gattttattg ttgagtagtg
     4201 ttacgatgtt aggggtttta gcaacaaata atataattat atttattgtg atgaatataa
     4261 ttttctttgc attgaacgca atcattatac cagtgactca agatttagta gctagccgtt
     4321 ctaatgatac taatagtagt attgtaatgg gattttacaa cgcgataaaa tcattgggtg
     4381 gaattatagg tgccttgatg gctggtttct tttatacatt taatcctcgc ttaccattta
     4441 tttttggttt tgttgctttc cttttagcaa ctgttttatc atactactat tatcagcaaa
     4501 ataaattgca caaaagttaa aacattgtta taaatgtttt gatttttttt atttaacaag
     4561 agtagaataa aattatagga aatagtaaag ctaatgaatc atgatgaggc tacgtttttt
     4621 agtataatta gtattatggt aataaataag attaaaaata aataaatctg gttagcttaa
     4681 tgcttgcaca accagattta gcagaaattc attatttcaa agaagtgtgt tagtatgtag
     4741 aagtaaataa ctaaaactaa ctatattatt tttgatgttt tgttgaaact aaaatatttt
     4801 ttattaatac ttcattatct ttagtttttt caaacatatt taataatgaa tcagttgctt
     4861 gttcgatcgt agtttgatct aaagattttc gaattatttg cattgcttca tattcatctt
     4921 tgcttaataa taaatcttct cgacgagtac cggatttttg tacgtcaatc gcgggaaaaa
     4981 ttctcttttc ttgtaattta cggcttagta ctagttccat atttccagta cctttaaatt
     5041 cttcataaac catatcatcc atccgacttc ctgtttctat taaagcagta gctaagatag
     5101 ttagactacc accttcacgt gtatttcttg cagctccaaa taagcgttta ggaaaataaa
     5161 atgatgcagg atctagacca cctgataagg tccttcctga aggggtaaca gttaaattat
     5221 ttgctctagt taatcgtgtt aagctgtcta acaagatcat tgtatcatat ccttgttctg
     5281 ttaagcgttt agctcgttca agggtagttt gtgcaacttt gatatgactt tctgattttt
     5341 catcaaaagt agagtagata acttcaacat tatcagcatc caatgcttct ttaatatcgg
     5401 ttacttcttc aggtcgttca tcaataagta agataaatat cttgatgttt ggataatttt
     5461 ttgatacaga taaagcaata tcttttagta atgtcgtttt tccagctttt ggtggtgcaa
     5521 caataattcc tcgttgacct tttccaattg gagaaattaa gtcaattaga cgcattgata
     5581 attgttcatt atctttacct aaatatactc tttcattagg ataaatcggt gttaaaaatt
     5641 caaaagcaat tctttttttt gcttgatctg gattttgtcc gttaactaaa tcaactcgca
     5701 taagtgcatt atatcgttca ccatttttag aattacgtgc atgaccaact atttcatcgc
     5761 cattttttaa attaaagcgt cttatctgtg attgggaaat gtaaatatca cgactttctt
     5821 gagtataatt tttaggtctt aaaaatccat agccatcaac cattatttca agaattccag
     5881 atgctaattc acctaattcg tcttcttctt tggtatttac cttattttta tcatttttta
     5941 tactttccat agtatctcct tcttattgtt ttattacaat taatattcat tataatttag
     6001 cttgctagta ttatacaata aaatttatta atattcgtga ttgtcttttt cattatttgc
     6061 aaatatcatt ctaaactaat taatatcgtg gtatgattct atcaatgaaa gattggggga
     6121 ttatcatgga tattatggta gttattaacc aaatgattca attattttta attattggac
     6181 ttggttattt tatgcaaaaa aagaaaatat taaatgatga attaaatacg aaactaaatt
     6241 ttattgttat ttcaattact acaccagcac ttattttttc atctgtttgt acaacatcga
     6301 ttagtgaaaa gagtatggtc atttatactt tagcaattgc tacagcagtg tatgtggcct
     6361 taccggtcat cagttttgtt ttagttaaat taatgcgaat tccaatgcac caaaaaggat
     6421 tatacatgtt tatgacaatt ttttctaata ctggatttat ggggtatcca attatgaaag
     6481 ccttgtttgg taatgacgca gttttttata ctgctttatt taatatcctt tttaatttag
     6541 aagtatttac tttaggcgtt attttaatta attatgggaa tgatgtaaaa atgaaattaa
     6601 atcctaaaaa tttactttca cccggagtag ttgcttcaat tattgcaata tttatttatt
     6661 ttttagaaat tccaattcct gatgtaattg ctaattcatg tggtatggtt ggggacatga
     6721 caacaccatt agcaatgatg atcattgggg caaccttagc taatattaaa gttaaagagt
     6781 tgttcacaga attaagatta tactacttta cgattgttaa acaagtt
//
LOCUS       DAOMTA010000046         6804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_165_length_6804_cov_2.262261, whole genome shotgun sequence.
ACCESSION   DAOMTA010000046 DAOMTA010000000
VERSION     DAOMTA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165_length_6804_cov_2.262261"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="PLK68_02185"
     CDS             <1..1965
                     /locus_tag="PLK68_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRM89249.1"
                     /translation="KTIEQLQKDGYDCIMNNPEDYLDTLLNAAKKNHHQLKIYQGEAR
                     SAALGRIHAGITSTRIDIKNAMRHYETMMAKVIEPMISISRFQGGYCDQELINYFWKI
                     IFKNQFHDSIYGSSPDSINHTVENRLLNLRHGLNELIWMNFRYLAESVDLSVLKENED
                     ILVLFNTLPYQRNDYAFTSMIVKDKNFVLKRQDGTIVPYEIMNEVKATSHDIEYYNGM
                     ENFHDAGEVLEGTKFKVQIKIAASFLPPMGYEVLKVCFHERTSKRPEGDVVILKNGAE
                     NKYLVMHIQEDGTLSVTNKQTQETYHQLNTFVEKGDDGDEYNYSPCIDDTEIRINDIH
                     PMITCIESSSIEVKYRLEYQIQVPEKVVDHHRVQALKPLKICSDVSLKANSQTIDFVT
                     KINNHSCDHIVRVCFEDIYKAAENCSQDQFGTIIRQNVIKNQKSLKDGATEYVLPIYA
                     MQRFVKLDHQKSIMAVLSKGPLEYEIENNQKICLTLLRSVGKFGKADLLIRPGRSSGY
                     RMDAPSSQLLNQTITSEYSLFFGTKEQMSKMIQIAEVINTPVQTRYLNDISRRENLKL
                     DWSYSAITLDERVEFMALKKAENSEASIFRILNNREVDVADVELFIPINKRCYLCDVQ
                     ENKQTEVENQNGKVIIKNIVSNTFVTVIIE"
     gene            2006..2767
                     /locus_tag="PLK68_02190"
     CDS             2006..2767
                     /locus_tag="PLK68_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89250.1"
                     /translation="MSLIKNFQKYGNNLTELEIECFNKLLTFRDLEPSLTISSLAETL
                     NVSTTTIFRMVKKLDYKTFMDFRYDLLYHRRDEYEYQSPVESTCDSLEKEIKDTIEML
                     RNLDIDDVIKDIVKAKSVLICSSGMNKYVAKILAVKLSLYGIRTIYPDDHWFLYLEAN
                     NLTQDDFVIVLSRGGATEAIIDVMKNAKLSGCKILLVTETRNSPMTKLSNYVMNVSVT
                     KDEGYDIDSRLHIHLAIEYLLRELMNQYLYNKKYL"
     gene            2785..4986
                     /locus_tag="PLK68_02195"
     CDS             2785..4986
                     /locus_tag="PLK68_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM89251.1"
                     /translation="MIEKIVKEMTLKEKVGQLNQHLYGWQCYQKVNGKYELTDLFKEH
                     VKEYGGVGAIYGILRADAWSQINRENGISREDSKIVITMIQEYIKKHSRFEIPALISE
                     ECVHGHMALGAPVFPTNLAMGMTWNPDLMERITHNVSQELAAKGGNLALFTGFDVLRD
                     PRWGRSEECFSEDAYLTSCMTSAAVHGFQKEKNGVAVVVKHLCAQGGCVGGHNSDAAL
                     IGPRELREIHLPPVKAAIDAGAKAVMAAYNEIDGIPCHINKALLFETLRKEYDFSGIV
                     MADGCALDRLLLLDDDILKVGSLALKAGVDLSLWDNVYLRLDEAIKQGYLTEEELDQA
                     VLRVLRLKEELGLWEELNTYSLPEASDLLIQAARECQVLLKNNDSLLPLSSKQKIAVI
                     GPNANHYLNQLGDYTAYQNKSDIVTVYDGICQKTEISPIYVQGCSIRGRKFDIEPALV
                     AAKAADIVILVLGGNSTRLYQDTFENNGAVKANQENQMNCGENIDLASLELEGYQNEL
                     LLALKEVNPHIVTVLIQGRPHVINTVLKHSQAVLASFYPGSRGGEGIADVLFGDYNPS
                     GHLSVSIPQHVGQLPCYYNHKHNGAQKDYVDMPSEALLPFGYGLSYSQFIYRDIKVPK
                     AIQITDLLENGIDLQLEIYNNSTYDGEDVIQVYLKDHQASVVSRVIELKAFNKVKIQA
                     YQSKQISIHLTSDAFAIWNYEMKYLVEPGDVSICIGVDSKHYQEFKLTLVK"
     gene            5164..6468
                     /locus_tag="PLK68_02200"
     CDS             5164..6468
                     /locus_tag="PLK68_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM89252.1"
                     /translation="MIERIIQLITFKEVIGSLMIFIAVLIVASIIWGNRTFSVKTLNQ
                     MIFHLKVPMDGTDDGIYTDWFLHTVPQSFVIVAFTEIIVFNLPLPAFHIYLIAHIFAI
                     GCFAIIGSLLFALYNYQIFGYAFDMLRKTQLYEEHYVDPKGVTLTFPSCKRNIIHIYL
                     ESIENAYLAKTSGGGQDVSYMPELDALANQNINFSHHNQIGGSLTLEGTQWTIASMVG
                     QEAGIPLLVPFNSKSYNDKSNFFSGVYTLGEILEQHGYINEIMMGSDSNFGCTSNFYK
                     QHGNYYISDYNTAVEEGRIAKDYFVFWGYEDKKLFEFAKADITKLAKSGKPFNMELVT
                     IDTHTPDGYVCEDCQHKYKSQYANVIACQSKQVNNFINWCKSQPWYENTTIVITGDHN
                     SFLLILITIMFGHHTTALLIVQLLQNLIKTENFQLLICILLF"
     gene            6474..6671
                     /locus_tag="PLK68_02205"
     CDS             6474..6671
                     /locus_tag="PLK68_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89253.1"
                     /translation="MGVKIDGNKLGLGVNLFSGEKTLIEQYGYRKINQEVKKKSRYYR
                     HKLIGDDIKEREQKELSKRSD"
BASE COUNT         2465 a          847 c         1291 g         2201 t
ORIGIN      
        1 aagacaattg aacaattgca aaaagatggt tatgactgta ttatgaataa ccctgaggat
       61 tatcttgata cactgcttaa tgcagcaaag aaaaatcatc atcaattaaa aatttatcaa
      121 ggtgaagcaa gaagtgctgc actaggtcga attcacgctg gtatcacctc aactagaatt
      181 gatataaaaa atgcaatgcg tcattatgaa acaatgatgg caaaagtaat tgaaccaatg
      241 attagtatct cgcgttttca aggaggctat tgtgatcaag aattaattaa ttatttttgg
      301 aagattattt ttaagaatca atttcatgat tctatctatg gttcttcacc agatagtatt
      361 aaccatactg tagaaaatcg tttattaaat ttaagacatg gtttaaatga gctaatctgg
      421 atgaatttcc gttatttggc agaatcagtg gatttgagtg tactaaagga aaatgaggac
      481 atcttagttc tatttaatac tcttccttat cagcgtaatg attatgcttt tacaagcatg
      541 attgttaaag ataagaactt tgtgttaaaa cgtcaagatg gaacaatcgt cccttatgaa
      601 ataatgaatg aagttaaagc aactagccat gatattgaat attataatgg aatggaaaat
      661 tttcatgatg caggagaagt tttagaagga acaaaattta aagtacaaat taagattgcg
      721 gcatctttct taccaccaat gggctatgaa gttttaaaag tgtgtttcca tgaaagaact
      781 tcaaaaagac cagaaggaga tgttgtcatt ttaaaaaatg gtgctgaaaa taagtattta
      841 gtgatgcaca ttcaagaaga tggaacgctt tctgttacga ataaacagac tcaggaaaca
      901 tatcatcagt taaatacttt tgtcgaaaag ggggatgatg gtgatgaata taattattct
      961 ccatgtatag atgatacaga aataagaatt aatgatattc atccaatgat tacttgtatt
     1021 gaatcatctt ctattgaagt aaagtatcgg cttgaatatc aaattcaagt tcctgaaaaa
     1081 gtagttgatc accatcgtgt acaagcatta aaacccctta aaatatgtag cgatgtctct
     1141 ttgaaggcaa acagtcaaac aatagatttc gtaactaaaa ttaataatca ttcatgtgat
     1201 catatcgtaa gagtatgttt tgaagatatt tataaagcag cagaaaactg tagtcaagat
     1261 caatttggaa caattatccg tcaaaacgta ataaaaaatc aaaagagttt aaaagatggt
     1321 gccactgagt atgttctgcc aatatatgca atgcagcgtt ttgttaaact agatcatcaa
     1381 aaatcaatta tggcagtatt aagtaaagga ccattggaat atgaaattga aaataatcaa
     1441 aaaatttgtt taaccttatt aagaagtgta ggaaaatttg gtaaagcgga tctattgatt
     1501 agacctggac gttcttcagg atatcgtatg gatgcaccat cttcacaatt attaaatcaa
     1561 actataacaa gtgaatattc attgtttttt ggaacaaaag aacagatgag taagatgatt
     1621 caaatagcag aagtaataaa tacaccagtt caaacacgct atcttaatga tataagtaga
     1681 cgtgaaaatt taaaacttga ttggtcttat agtgcgatta cgttggatga acgagtggaa
     1741 tttatggctt tgaaaaaagc tgaaaatagt gaagcttcta tcttccggat tttaaataat
     1801 cgtgaagtag atgtcgcgga tgttgagctt ttcattccta ttaataaacg atgttattta
     1861 tgtgatgttc aagaaaataa acaaacagaa gttgaaaatc aaaatggaaa ggtaatcatc
     1921 aaaaatattg tgtcaaatac tttcgttact gttataatag agtagatttt ttctactcta
     1981 ttttcatata ggaagggtaa tgcatatgag tttaattaag aattttcaaa aatatggaaa
     2041 taatttaaca gaattagaaa ttgaatgttt taataaatta ttaacgtttc gggatttaga
     2101 accaagtttg acgatttctt ctttagcaga aacattaaat gtatctacaa cgactatttt
     2161 taggatggta aagaaattgg attataaaac atttatggac tttagatatg atcttttata
     2221 tcatcgtcga gacgaatatg agtatcaatc tccagttgag agtacatgtg attccttgga
     2281 aaaagaaatt aaagatacga ttgagatgtt acgcaatcta gatattgatg atgtcattaa
     2341 ggatattgtt aaagctaaaa gtgttttaat ctgttcttca ggaatgaaca aatatgttgc
     2401 aaagatatta gcagtgaagt tgagtttata tggaattaga acgatttatc ctgatgatca
     2461 ctggttttta tacttagaag ctaataattt gactcaggat gactttgtaa ttgtgctatc
     2521 aagaggtggt gctactgagg caattatcga tgtaatgaaa aatgccaaac ttagtggttg
     2581 taaaatatta ttggttacag aaacgagaaa ttccccaatg acgaaactgt caaattatgt
     2641 gatgaatgtt tctgtaacta aggatgaagg atatgatatt gattcacgtt tgcatatcca
     2701 tttagcaata gaatatttat taagagaatt aatgaatcaa tacttatata ataaaaaata
     2761 tttataagaa atgagaaaat agagatgatt gaaaaaattg taaaagagat gacgttgaaa
     2821 gagaaagttg gtcaattaaa tcaacattta tatggatggc aatgttatca aaaagttaat
     2881 ggaaaatatg aattaacaga cttatttaaa gaacatgtta aggaatatgg tggcgttgga
     2941 gcaatttatg gcattttacg tgctgatgcc tggtctcaga tcaatagaga aaatggaatt
     3001 agccgtgaag atagtaaaat tgtcattaca atgattcaag aatatataaa aaaacattca
     3061 cgttttgaaa tcccagcatt aattagcgaa gaatgtgtgc acggacatat ggcattaggg
     3121 gcaccggtat tcccaactaa tttagcaatg gggatgactt ggaatcctga tttgatggaa
     3181 agaattacgc ataatgtgag ccaggaactt gctgccaaag gtggaaattt ggcattattt
     3241 acgggatttg atgttttaag agatcctaga tggggccgta gtgaggaatg ttttagtgag
     3301 gatgcttact taacaagctg tatgacaagt gcagcagtac atggattcca aaaagaaaag
     3361 aacggtgtag ctgtagttgt caaacattta tgtgcgcaag ggggttgtgt aggtggacat
     3421 aattcagatg ctgcattaat tggacctcgg gagttgcgag aaattcattt accaccagtt
     3481 aaagcagcga ttgatgctgg cgctaaggca gttatggctg catataatga aatagatgga
     3541 attccttgtc atattaataa agcattgctt tttgaaaccc ttagaaaaga atatgatttt
     3601 tctggtattg ttatggctga cggatgtgct ttagatagat tattgttgtt ggatgatgat
     3661 atcttaaaag ttggcagttt agcacttaaa gcgggagtag atctgagttt atgggataat
     3721 gtttatttac gattagatga agcaataaaa caaggttatt taactgaaga ggaacttgat
     3781 caagcagtat taagggtatt gagattaaaa gaagaattag gattatggga agaactaaat
     3841 acttattcat taccagaagc atcagattta ttaattcagg ccgctcggga atgtcaagta
     3901 ttattgaaaa ataatgattc acttcttcca ttatcctcaa aacaaaaaat tgctgttatt
     3961 ggtccaaatg ctaatcacta cttaaatcaa cttggagact atactgctta tcaaaacaaa
     4021 agtgatattg ttacagtgta cgatggtatt tgccaaaaaa cagaaatttc ccctatctat
     4081 gtacagggat gttcaatacg gggaagaaaa ttcgatattg agcccgcact cgtggcagct
     4141 aaagcggcgg acattgttat tttagtatta ggcggtaata gtacaagatt atatcaagat
     4201 acctttgaaa acaatggtgc ggtaaaagca aatcaagaaa accaaatgaa ttgtggggaa
     4261 aacattgatt tagcttcatt agaactagaa ggttatcaaa acgagctttt attagctttg
     4321 aaagaagtga atccacacat agtaacagta ttgattcaag gcaggccaca cgtcataaat
     4381 acggttctaa agcattctca agcagtttta gcaagttttt atcctggtag tagaggcggt
     4441 gagggaattg ctgatgtctt gtttggagat tataatccaa gtgggcattt aagtgtttct
     4501 ataccgcaac atgtaggaca attaccttgt tattacaatc ataaacataa tggagcacaa
     4561 aaagattatg ttgacatgcc cagtgaagcg ctgttacctt ttggttatgg cttaagttat
     4621 tcacaattta tttatcgaga tatcaaggta cctaaagcca ttcaaataac cgatttactt
     4681 gaaaatggaa ttgatttgca gttagaaata tacaataaca gtacctatga tggtgaagat
     4741 gtaatacaag tttatttaaa ggatcatcag gcttcagttg tttctagagt aattgaactt
     4801 aaggctttta ataaagtaaa aattcaagcc tatcaatcaa aacagataag tattcattta
     4861 acttcagatg cttttgctat ttggaattat gaaatgaaat atcttgttga accaggtgat
     4921 gtttcaattt gtatcggagt cgattctaag cattatcaag aatttaagct tactcttgta
     4981 aaataaatat agtattagtc attttattat tatctaataa aaatatactt tattaattta
     5041 aaattcacat aagaagagta aggtaaatgt aaaaatatgg taaagtttgg tgaatgaaat
     5101 ttttgtttta ggtctattta tatgctagaa taaatagaaa taataagaag aaagatggtt
     5161 gtgatgatag aaagaataat acagttaatt acatttaaag aagtgattgg atcattgatg
     5221 atatttattg cagttttgat cgtagcctca attatttggg gaaatcgaac tttttctgtg
     5281 aagacattaa atcaaatgat ttttcattta aaagttccta tggatggtac agatgatgga
     5341 atttatactg actggtttct tcacacagtt cctcagagtt ttgtaattgt tgcattcaca
     5401 gaaataatcg tttttaatct acctttacca gcatttcata tatatttaat agcgcatata
     5461 tttgcaattg gctgttttgc aattattggt tctttgctct ttgctttgta taattatcaa
     5521 atttttggat atgcttttga tatgcttaga aaaacacaat tatatgaaga acattatgtt
     5581 gatcccaaag gggtaacgtt aacttttcca agctgtaaac gaaatattat tcatatctat
     5641 ttagaatcta tcgaaaatgc ttatttagca aaaacatcgg gtggtggtca agatgtttct
     5701 tatatgcctg aacttgatgc attagcgaat caaaatatta acttttctca tcataaccaa
     5761 atcggtggaa gtttgacctt agagggaaca cagtggacga ttgcttcaat ggttggccaa
     5821 gaagcaggaa ttccgctatt agtaccattt aattcaaaat catataatga taaatcgaat
     5881 ttttttagtg gtgtttatac acttggtgaa attttggaac agcatggtta tataaatgaa
     5941 atcatgatgg gttctgatag taattttggg tgtacatcaa atttttataa acaacatggt
     6001 aactattata tttccgatta caataccgca gttgaggaag gaagaattgc taaagattat
     6061 tttgtttttt ggggttatga agataaaaag ttatttgagt ttgctaaagc ggatattact
     6121 aaattggcta aaagtggtaa accttttaat atggagctag tcacgattga tacgcataca
     6181 cctgatgggt atgtatgtga agattgtcag cataaatata aaagccaata tgcaaacgta
     6241 attgcctgtc aatcaaaaca agtgaataat tttataaatt ggtgcaagag ccagccatgg
     6301 tatgaaaata caactatagt tattactggt gatcataata gttttttact aatcttgatc
     6361 acaattatgt tcggacacca tacaactgct ttattaatag tgcagttact acaaaattta
     6421 ataaaaaccg aaaattttca attattgata tgtatcctac tattttagca gcaatggggg
     6481 taaagataga tggaaacaaa ttaggtttag gagttaatct tttttctggt gagaaaactt
     6541 tgattgaaca atatggttat agaaagatta accaagaggt caaaaagaaa tctagatatt
     6601 atcgtcataa gttaattggt gatgatataa aagaacgtga acaaaaagaa ttaagtaaac
     6661 gaagtgatta aaatcgtaca ctaaaaatag tgtacggttt taatcgtttt ttatttaaaa
     6721 acattgacta tacgcttaga caatagttta gagtaaagga agaaaaggat attattaaag
     6781 catcttttta ataaaaaagg aggg
//
LOCUS       DAOMTA010000047         6793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_166_length_6793_cov_2.210003, whole genome shotgun sequence.
ACCESSION   DAOMTA010000047 DAOMTA010000000
VERSION     DAOMTA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_6793_cov_2.210003"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>456)
                     /locus_tag="PLK68_02210"
     CDS             complement(<1..>456)
                     /locus_tag="PLK68_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89254.1"
                     /translation="NYYFDIAKKIFDSEDDYDDFKIVSSKTSKVLYATKYSYNTINDN
                     NEAVNKNATIRQILFNDNIVYLIYNYHLVGISTKDYQVVFDYEAKDNIWKMALVNNQV
                     IFTTQLGELGSLNKTNGKLNYTVALTTPKEIKDPRYDTLGKVNLNIWDIV"
     assembly_gap    457..466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<467..793)
                     /locus_tag="PLK68_02215"
     CDS             complement(<467..793)
                     /locus_tag="PLK68_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89255.1"
                     /translation="MKKMLTFLIATILILAMSLMPLQAITGINSLVSYGEGNEIEGGC
                     NGKNVDLGEGLSGLMVSNQSELVFIGNDDFKIKVGKPVKEFEIIDDVDNDGLKDVAVY
                     IDSEDDY"
     gene            1084..1512
                     /gene="brxF"
                     /locus_tag="PLK68_02220"
     CDS             1084..1512
                     /gene="brxF"
                     /locus_tag="PLK68_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HRM89256.1"
                     /translation="MNPLTAEIATAQNSDNKLILIIGGPGSGKSKLIHDYSNETGIPI
                     LNLDQIFKDDCSEITTVMNDFIDNYDKEVLLLDNKRVLYAKDSNIDMLTFLKELAKKI
                     IVVATWNGTIADNKLIHIRSKLPANLEYSLKNEQIKFIKC"
     gene            complement(1530..2138)
                     /locus_tag="PLK68_02225"
     CDS             complement(1530..2138)
                     /locus_tag="PLK68_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRM89257.1"
                     /translation="MALKVVPTKGNLIAMKKSLQLANLGYNLMDQKRNVLIKEMMTLL
                     DDVKLIRDQITSSYQEAYDALQEANISMGLITDIVNSTPEDYGISIAYRSVMGVEIPK
                     IAYDKQPLKMTYDIERSNSKVDYAYNCFYNVKQLTVLLAEVENSVYRLANTIRKTQKR
                     ANALRNISIPRFESTIKVISEALEEKEREEFTRQKVIKEMKK"
     gene            complement(2141..3583)
                     /locus_tag="PLK68_02230"
     CDS             complement(2141..3583)
                     /locus_tag="PLK68_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRM89258.1"
                     /translation="MSLQYVGLNEINGPLVVLDHVKGASYDEMVDIQLKDGTTRAGRI
                     VQIEGEKVVVQVFEGTNGLSLENTKTRLLGKPMELPVSKEILGRIFSGAGRPIDGLGE
                     VYAEAEMDINGLPLNPVSRVYPRNYINTGISSIDCLTTLIRGQKLPIFSVSGLPHDRL
                     AVQIVKQAKIADEAGKGFAVVFAAMGVTNDVAEYFKRSFKEAGVMERVVMFLNLSNDP
                     IIERILTPRCALTAAEYLAFKQNMHVLVIYTDVTSYCEALREFSSSKGEIPGRKGYPG
                     YLYSDLASLYERAGIIKDAEGSVTQIPILTMPNNDITHPVPDLTGYITEGQITLDVDL
                     NSAGVYPPVGVLPSLSRLMKDGIGEGFTRADHQDIANQLFACYAHVQDVRSLTSVIGE
                     DELSDMDKKYISFGRLFEEYFINQGFDTNRSIEETLDLGWDLVSVLPREALDRLDNAL
                     LDQYYNHEKAVERFNIKEKKIIKELQEEGE"
     gene            complement(3597..5348)
                     /locus_tag="PLK68_02235"
     CDS             complement(3597..5348)
                     /locus_tag="PLK68_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRM89259.1"
                     /translation="MNNNVIYSINGPVVKVKDTVSFSMLEMVYVGNDSLMGEVISISK
                     EFTTIQVYETTTGLKPGEPVVSTGSPICATLGPGILRNIFDGIERPLKALEKESGAFI
                     QAGSNVDSLDIAKKWDVTMTVKVGDEVKGGTIYATTPETDLIVHKCMVSPLISGKVVE
                     VVEDGQYTINDVVMKIEDAHGKIIECTLCQKWPIKQSRPVTKRLPIGTPLVTGQRVID
                     TLFPIAKGGTAAIPGGFGTGKTMTQHQIAKWCDADIIVYVGCGERGNEMTQVLEEFSE
                     LIDPKSGKPLTDRTVLIANTSNMPVAAREASIYTGVTLAEYYRDMGYHVAIMADSTSR
                     WAEALREISGRLEEMPAEEGFPAYLPSRLSQFYERAGYMETLNGQEGSVSIIGAVSPQ
                     GADFSEPVTQNTKRFVRCFWALDKALAYARHYFAINWTQSYSDYVYDLEKWYSKNVGP
                     KFVSDRQEIAYILAEEAKLNEIVQLMGPDVLPEDQKLIIEVAKVVRVGYLQQNAFHKD
                     DTYVPMEKQLKMMEVILYLNKRCKEAVNQGKVVRRIVDTGIFDQVIKMKYDIPNDNIA
                     KLDTYYHDIDEAIKSVA"
     gene            complement(5364..5960)
                     /locus_tag="PLK68_02240"
     CDS             complement(5364..5960)
                     /locus_tag="PLK68_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRM89260.1"
                     /translation="MEKKEQVFLYMKEEIERLANLEEEKILDEAKRLEEEAYNQIKAE
                     AKKDAEALLAKELVEISSNASVEASSSQEEKTKKLVEKRDEYVANIFSEAKDKLVAFA
                     NSKDYQAYLVKHMEEIGKLYPMSDSTLELREADMKYKDELIKAYGTALEVEVSDKITI
                     GGFIVKNKATNVVVDESLDFALENQKDWFYKTSGLMIK"
     gene            complement(5973..6281)
                     /locus_tag="PLK68_02245"
     CDS             complement(5973..6281)
                     /locus_tag="PLK68_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRM89261.1"
                     /translation="MRFYCISDNVDTQVGLRLVGIEGQVVHKRREFLELLETKLKDDS
                     IGIILITTNLIELAPDVISEIKLKQQKPLLVEIPDRHGESKIGETIDKYVSEAIGVKL
                     "
     gene            complement(6301..6720)
                     /locus_tag="PLK68_02250"
     CDS             complement(6301..6720)
                     /locus_tag="PLK68_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRM89262.1"
                     /translation="MTVLEFILPALVVILLSLPLIKVFTGKVSVKSAKIRLATHVAGF
                     AGALYLAAVTNGVYAEETAVITGTIAQGLGFVAAALATGLSALGAGIAVAAAAPAAIG
                     AFSENEKNFGKSLIFVALGEGVAIYGLLISILIINKI"
BASE COUNT         2185 a         1320 c          917 g         2361 t
ORIGIN      
        1 acaatatccc aaatatttaa attgactttc cccaaagtat catatcgtgg atctttaatt
       61 tctttaggtg ttgttaatgc aactgtataa tttaattttc cattagtttt attaagactt
      121 cccaattctc ctaattgtgt cgtaaaaata acctgattat taactaatgc cattttccaa
      181 atattatctt tcgcttcata atcaaataca acctgataat cttttgtact aattcctact
      241 aaatgataat tataaattaa atagacaata ttatcattaa atagaatttg tctaattgtg
      301 gcattcttat tcactgcctc attattatca ttaatcgtat tataagaata ttttgtagca
      361 tataatactt ttgaagtttt tgaactaacg attttaaaat catcataatc atcttcacta
      421 tcaaaaatct ttttagcaat gtcaaaatag tagtttnnnn nnnnnnataa tcatcttcac
      481 tatcaatata aacagcaaca tctttaagac catcattatc aacatcatca attatttcaa
      541 attctttaac cggcttacca actttgattt taaaatcatc gttaccaata aaaactaact
      601 ctgattgatt agataccatc aatccgctta gtccttcgcc taaatcaaca tttttcccat
      661 tacagccacc ttcaatttca ttaccttcac cataactaac taagctattt atgccggtaa
      721 ttgcctgtag tggcatcaac gacatagcca aaatcaaaat cgtagcgatt aaaaaagtaa
      781 gcattttctt cattctaagc atctcctttt aatatcatgt ttcataacaa aactgtatca
      841 ttccctatag tacagtttca ttatatcatc gcattaactg tatcacaata gatagtacac
      901 ctttttagaa aaactttaga aatacttaat gactataaat cgtcaatatt tcctaggaaa
      961 tactagtaaa attatgatat tgctaggaaa tgacaaatat aaatgataga atgaaaatag
     1021 ataataagtc gattttaaca aggttttttg gattaaagag aatataaaaa ggaggacaga
     1081 cccatgaacc cgttaacagc agaaatcgcc accgcccaaa atagtgataa taaactaatt
     1141 ttaattatcg gtggtcctgg gagtggtaaa tcaaaactta ttcatgacta tagcaatgaa
     1201 accggtattc caattctaaa tcttgatcag atctttaaag atgattgtag tgaaatcacc
     1261 actgtaatga atgactttat tgataactac gataaagaag ttcttttgtt ggataataaa
     1321 agagtccttt atgctaagga ctcaaatatc gatatgttaa cattcttaaa agaattagct
     1381 aaaaaaataa ttgttgtagc aacatggaat ggtactattg ctgataataa actaattcat
     1441 attcgttcta aactaccagc taatcttgaa tattctttaa aaaatgaaca aattaaattt
     1501 attaaatgtt aaaaatagaa gcatagcttc tattttttca tttctttaat aactttttga
     1561 cgtgtaaatt cttctctttc tttttcttcc aacgcttctg aaataacctt aatagttgat
     1621 tcgaagcgcg gaatcgaaat atttcttaaa gcgttagcac gtttttgggt tttacgaatc
     1681 gtattagcta aacgataaac actattttct acctctgcta ataaaactgt taattgtttt
     1741 acgttgtaaa aacaattata agcataatcg acttttgaat ttgaacgttc aatatcataa
     1801 gtcatcttta agggttgttt atcataagca atcttaggaa tctcaacccc cataacactt
     1861 cgataagcaa ttgaaatccc ataatcctca ggtgtactat taacaatatc tgtaattaac
     1921 cccattgaaa tattagcctc ttgaagtgca tcatatgctt cttgatatga agaagtaatt
     1981 tgatcacgaa taagttttac atcatctaat aaagtcatca tttctttgat taaaacgtta
     2041 cgtttttgat ccattagatt atatccaagg ttcgctaatt gcaatgattt tttcattgca
     2101 attagattac ctttagttgg aactactttc aaggccatga ttattcacct tcttcttgta
     2161 attctttaat aatcttcttt tctttaatgt taaagcgttc aaccgctttt tcatgattat
     2221 agtattgatc taacagagca ttatctaaac ggtctaaggc ctcacgagga agaactgaaa
     2281 ctagatccca tcctaaatct aatgtttctt caatactacg gttagtatca aaaccttggt
     2341 taataaagta ttcttcaaat aaacgaccaa agctaatgta ttttttatcc atatctgata
     2401 attcatcttc accaataact gatgtcaatg aacgaacatc ttgaacatgg gcatagcatg
     2461 caaataattg gtttgcaata tcttgatgat ctgcacgagt aaacccttca ccgattccat
     2521 ctttcattaa acgacttaat gaaggtaata ctcctactgg aggataaact ccagctgagt
     2581 ttaaatcaac atctaaagtg atttgccctt cagtaatata tcctgttaag tcaggtactg
     2641 gatgagtaat atcattatta ggcattgtta gaattggaat ttgggttact gaaccttcag
     2701 catctttgat gattcctgct ctttcatata atgaagccaa gtcagaatat agatatccag
     2761 gatatccttt acgaccagga atttcaccct tacttgaaga aaattcacgt aaagcttcac
     2821 aataagaagt aacatcggta taaattacta aaacatgcat attttgttta aatgctaagt
     2881 attcagcagc cgttaaagca caacgtggcg ttaaaatacg ttcaataatt ggatcatttg
     2941 aaagatttaa gaacatgact actctttcca taaccccagc ttctttaaaa ctacgtttaa
     3001 agtattcagc tacgtcatta gttaccccca tagctgcaaa aacaacagca aatcctttac
     3061 ccgcctcatc agcaattttc gcttgtttta caatttgaac tgctaaacgg tcatgtggta
     3121 aaccagatac tgagaaaatt ggtagcttct gtccacgaat caatgtcgtt aaacagtcaa
     3181 ttgaagaaat tccagtatta atatagttac gtggatatac ccgtgataca gggtttaatg
     3241 gcaatccatt gatatccatt tcagcttcag catatacttc acctaaacca tcaattgggc
     3301 gaccagcacc agagaaaatt cttccaagaa tttctttaga aactggtaat tccatcggtt
     3361 tacctaataa ccttgttttt gtattttcaa gtgacaagcc atttgttccc tcaaatactt
     3421 gaacaacaac tttttcacct tcgatttgaa caattcgacc agctcgagtt gttccatctt
     3481 ttagttgaat atcaaccatt tcatcgtatg aagcaccctt aacgtggtct aaaacgacta
     3541 atggcccatt tatttcattt aagcctacat attgtaatga cataatcatt ttcctcctac
     3601 gctacagatt tgattgcttc atcaatatca tgataataag tatctaattt tgcaatatta
     3661 tcatttggaa tatcatattt cattttaata acttgatcaa agattccagt atctacgata
     3721 cgtcttacga ctttaccttg atttaccgct tctttacaac gtttatttaa atataaaatt
     3781 acttccatca tttttaattg tttttccatt ggtacataag tgtcatcttt atggaacgca
     3841 ttttgttgca aatatccaac tcgaactacc tttgcaacct caataattaa tttttgatct
     3901 tctggcaata catcaggacc cattaattga acaatttcgt tcaattttgc ttcttctgct
     3961 aagatataag caatttcttg acgatcactt acaaatttag gcccaacatt tttactatac
     4021 cacttttcaa gatcatagac ataatcacta taactttgtg tccagttaat tgcaaaatag
     4081 tgacgagcat aagctagagc tttgtctaat gcccagaaac aacgtacgaa acgtttagta
     4141 ttttgagtaa ctggttctga gaagtctgcc ccttgaggtg agactgctcc gataattgat
     4201 actgaacctt cttgtccatt taatgtttcc atatatccag cccgttcata gaattgtgat
     4261 aaacgagatg gtaaataagc tgggaaccct tcttcagcag gcatttcttc tagacgacca
     4321 gaaatttcac gaagtgcttc agcccaacga gatgttgaat cagccataat agcaacatga
     4381 taacccatat cacgataata ttctgctaaa gtaactccag tataaatact tgcttcacgt
     4441 gccgcaactg gcatgtttga tgtattggca attaaaaccg tacggtcagt aagtggttta
     4501 ccactcttag ggtcgatcaa ttcactaaat tcttcaagaa cttgagtcat ttcgtttcca
     4561 cgttcaccgc acccaacata aacgataatg tctgcatcgc accatttagc aatttggtgt
     4621 tgggtcattg ttttacctgt accgaaacca cctggaatag cagctgttcc tcctttagca
     4681 atcgggaaca gcgtatcgat aacccgttga ccagttacta gaggtgttcc aattggtaat
     4741 cgttttgtga ctgggcgtga ttgtttaatt ggccattttt ggcataatgt acattcaatg
     4801 atcttaccat gagcatcttc aattttcatg accacatcat tgattgtata ttgaccatct
     4861 tcaaccactt caaccacttt accactaatc aatggactaa ccatacattt atgaacgatt
     4921 aaatcagttt caggtgttgt tgcataaata gttccacctt tgacttcatc gccaacttta
     4981 acagtcattg taacatccca ctttttagca atatctaaag aatcaacatt gcttccggct
     5041 tggataaatg ctcctgattc tttttcaagt gcttttagtg gacgttcaat cccgtcaaag
     5101 atatttctta aaattcctgg gcctaacgtt gcacagattg gcgaaccagt tgaaacaact
     5161 ggttcacctg gcttaagacc agtagtagtt tcatatactt ggatggtagt aaattcttta
     5221 gaaattgaaa ttacctcacc cattaatgaa tcattaccta catataccat ttccaacatg
     5281 gaaaaagaca ctgtatcttt tacttttaca acgggtccgt taatagaata aattacatta
     5341 ttgttcatat attaaccccc tgtctattta atcattaatc ctgatgtttt atagaaccaa
     5401 tccttttggt tttctaaagc aaagtctaat gattcatcaa caacgacatt agtagcttta
     5461 tttttaacaa taaatccacc aatcgtaatc ttatcactta cttctacttc taaagctgta
     5521 ccataagctt taatcaattc atctttatat ttcatatcag cttctctaag ttcaagagtt
     5581 gaatcactca ttggatataa tttcccaatt tcttccatat gtttaactag atacgcctga
     5641 taatctttgc tatttgcaaa agcaactaat ttatctttag cttcactaaa gatattagct
     5701 acatattcat ctcttttttc aactaacttc ttagtctttt cttcttgtga cgaagaagct
     5761 tcaacactag catttgaaga aatttctact aactctttag caagtaatgc ttcggcatct
     5821 ttcttagctt cagctttaat ttggttgtat gcttcttctt caagacgctt tgcttcatct
     5881 aagatttttt cttcttcaag atttgctaat ctttcaatct cttctttcat ataaagaaat
     5941 acttgttctt tcttttccat agcattcccc ttctataact tcacaccaat tgcttctgaa
     6001 acatatttgt caatcgtttc accaattttt gattcaccat gacggtctgg tatttcaaca
     6061 agcaatggtt tttgttgttt taacttgatt tctgagataa catcaggcgc aagttcaatt
     6121 aagttagtag tgatcaagat gattccgatc gaatcatctt ttaattttgt ttctagtaat
     6181 tcaagaaact ctcttctttt atgaactact tggccttcaa ttcctactaa tctaagtcct
     6241 acttgggtat cgacgttatc acttatacaa taaaatcgca taatgatcac attctttcaa
     6301 ttagattttg ttgataatta agatagaaat taataacccg taaatagcaa ccccttcacc
     6361 taaggctacg aaaattaatg atttaccaaa gttcttttca ttttcagaaa atgctccgat
     6421 agccgcagga gcagcagcag caacagcgat cccagctcct aaagctgata aaccagtagc
     6481 taaagcagca gctacgaaac ctaaaccttg agcaattgtt ccagtaatta cagctgtttc
     6541 ttcagcgtat acaccgttag ttacagcagc caaatataat gctccagcaa atccagcaac
     6601 atgagttgct aatctaattt tagcgctttt tacgcttact tttcctgtga atactttaat
     6661 taatggtaat gataataaga taactactaa tgctggtaaa ataaattcta aaactgtcat
     6721 aatgacttcc tcctcgattt ttaatcctct ttaatagttt taaatgaaat tccgtttcct
     6781 tcgtagtatc tac
//
LOCUS       DAOMTA010000048         6786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_167_length_6786_cov_2.365473, whole genome shotgun sequence.
ACCESSION   DAOMTA010000048 DAOMTA010000000
VERSION     DAOMTA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167_length_6786_cov_2.365473"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1535
                     /locus_tag="PLK68_02255"
     CDS             48..1535
                     /locus_tag="PLK68_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM89263.1"
                     /translation="MDFVQTGWALLPPVIAILIALKTKEVYLSLFFGIVSGALLLSNF
                     HIIDTVNVMFDTMIGSLSDTWNIGILIFLIFLGIIVTLMTKAGGSRAYGLWAKEKIKS
                     RQGSLFSTFLLGILIFVDDYFNCLTVGSVMREVTDEFSVSRAKLAYIIDSTAAPICII
                     APISSWAAAVSGYTSGDGFSLFLQTIPFNLYALLTIVMVTYVIKKDFHIGTMKHHEIA
                     AQNGDVHNGVNDYEEGEEMEVYDLLLPVFFLIISCIVGMLYTGGFFEGVSFITAFTNC
                     DASRGLVLGSFFTLVFIFILYLPRKIISYEDFAKCIPMGFKTMVPAMIILTLAWTLGD
                     LVSDKLQAGVFVYNLMQGASISTAILPVCLFLVGTGLAFATGTSWGTFGILIPIATSI
                     FPEGSQMLVIAIASILAGAVCGDHISPISDTTIMASAGAQCNHVNHVTSQIPYAMLVA
                     GACVVGYLVAGFTTNVVLTFVIAIIALFIFIFITKYFYNFKAKKD"
     gene            1633..4497
                     /locus_tag="PLK68_02260"
     CDS             1633..4497
                     /locus_tag="PLK68_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM89264.1"
                     /translation="MEESHNFYLNDYTEFVKQATDIIESNKNSKNYVIMYADLTNFQL
                     VNDFYGFIEGDRFLLEFAKFLSMSPRTILCGRVFSDHFLRLSLFEDNCDIESVTRNYE
                     QKLETFISSQEEHHPDSKIAVAAGLCPVKLEEKAIIKAIDQANIARKEAKKDNHCKLV
                     WFNEDMQKFINKRKILEIEIQNALRNKLFTFYLQPKVNINTGKIVGSEALARLIKDNK
                     VIYPDQFIEIMEKVCRYLRERIDQGKKLIPVSMNMSRMHVYETGFVARVNKIVNKYNI
                     PPYLLEFELTETVILKNLTKVREVADELRSFGYRVSIDDYGTGYSGMNIWQDLNFDIV
                     KLDRSYITKRESNNNRNNIIIPAIVDICNKLQTTIICEGVETLDQCLYMKRFGCNVVQ
                     GYYFSKPVSTVEFEKMIDETDGKFNLPWNKDDFKYTSVDNISQSEIKAITNSIFNFIP
                     GGVAGFSYELELLFVSDSLVELSGYSREELLSGGIEWPKKLFYSEEEFKHVFYHNGLA
                     DRSSKNLVEFKIKAKNGKTVYINMYGGLVDSPEWGSYYLCCFYDVTNEKVNELKSHEL
                     QENINSLLKNINGGIGKMTLDGKGTILFASDGFYKTIGYTRTDFNQPPINNDLSQIVS
                     KEAYFLCNHVIEQIKAGKIDYFEVEVNSKTAQKIWLTIYFSNIHRENGKLVADVFCID
                     STVDHQQMMLEQHCNEMEDNLNIVIENTPGDIVVIKIKDDHITTKFLSHGLSKIFDFE
                     QSELTDILKHNKGFDLVYEDDREVLAKQFIELSKQKSCVNFDYRSYYKNGKVSWHNIN
                     ANFYRQEEDGTIIYHGIITNINTLKEQQERAEKLKDRYNFILSVLAVDVWNFDVRNNR
                     MDSLYMDDKFSIDLPKGAKIVPEKLLEDALILPEDQNKYLNMLTSIRAGQKTAHDKLH
                     CRIKTGEYNLFDLMIRVKDTNSDMAIIMAKNFDQFFNKKE"
     gene            4630..5217
                     /locus_tag="PLK68_02265"
     CDS             4630..5217
                     /locus_tag="PLK68_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM89265.1"
                     /translation="MQIKKEDLKNDIIEAAKVEFLHHGYEGASMRIIASKSHTTLGNL
                     YNYFTNKEELLGAVLEPSIKSLNKLVEVHLNEEIQVHSLEEVDEALDQFGDFFEESDF
                     RYIMDERLIILFELKTTEYKEKRDWFLLKFKQHMAWHLNIEDINSPYIDIITNMFIDC
                     IKHVLVSHDNLEMQKQEFLKVFKMLCTGIVVNEEK"
     gene            5283..5978
                     /locus_tag="PLK68_02270"
     CDS             5283..5978
                     /locus_tag="PLK68_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89266.1"
                     /translation="MIKFEEVSKIYQNGNKKIYALNKVSFTINKGEFVIILGPSGAGK
                     STLLNILGGIDRLDEGKIIIDNEVISNKNENELSDYRAHQVGFIFQFYNLIPTLTVYE
                     NVALMKELKKDIMPVDNVLDQVGLLVHKKKFSHQLSGGEQQRVSIARAVVKNPEIFLC
                     DEPTGALDSDTGAKVLKLLHDMCKEYHKTTIIVTHNTNLQAIADKVIRVKNGQINTIT
                     TNSHPLDVMEVEW"
     gene            5979..>6786
                     /locus_tag="PLK68_02275"
     CDS             5979..>6786
                     /locus_tag="PLK68_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM89267.1"
                     /translation="MLLKKMIRDLWKNKVQFGAIFIMMFLGVFIFSGISSEYHGLEVA
                     LNNYIKENNLADAWLYQKEFSDSTLNELKKQTDYEQRMYVSTSNKAVDNSNIDLYITD
                     SNKISTLKVVNGKSFNNQDDGIWLDAMYAKKNGYQLNDQITLKYQNFELSKKIIGLVY
                     SPENIYLAKEEQLTPNRQKYGFAYVNNNSFPNTLYHPNQLVLSSKKDISKIVDQLLAD
                     NEYKLILQKDHSSVNMLTGEIAQHRSIGLVFSLAFLFIAVLITITTMHRVL"
BASE COUNT         2415 a          750 c         1234 g         2387 t
ORIGIN      
        1 agtactttga ttgttgccaa ttaaagtatt tttttatgga ggaaaagatg gattttgtac
       61 aaacagggtg ggcgttattg ccaccggtca ttgcaatttt aattgcatta aaaactaaag
      121 aagtttattt atcattattt tttggaattg tttcgggggc tttactgtta agtaactttc
      181 atattattga tactgttaat gttatgtttg atacaatgat tggaagttta agtgatacat
      241 ggaatatcgg tattttaata ttcttaatat ttttaggaat tatagttaca ttgatgacta
      301 aagcgggtgg aagtagagca tatggacttt gggcaaaaga aaaaataaaa tcaagacaag
      361 gctcactatt ttcaaccttc ttgttaggaa ttttaatctt tgtagatgat tattttaatt
      421 gtttaacagt tggaagtgta atgcgtgaag taacagatga atttagtgtt tcaagagcaa
      481 aattagctta tattattgat agtactgcgg ctccaatctg tattattgct ccaatttcta
      541 gttgggcggc tgctgtaagt ggttatacaa gtggtgatgg ttttagttta ttcttacaaa
      601 caattccctt taacttgtat gctttattaa caatcgtaat ggtgacttat gttattaaga
      661 aagattttca tattggaaca atgaaacatc atgaaatagc tgctcaaaat ggtgatgtgc
      721 ataatggtgt aaatgattat gaagaaggag aagaaatgga agtttatgat ctattattgc
      781 cagttttttt cttaatcatt agttgtattg tgggaatgtt atacacaggt ggtttctttg
      841 aaggagtatc attcattacg gcttttacta actgcgatgc ttcaagagga ctggtcttgg
      901 gatcattttt tacactagta tttatcttta ttttgtatct tcctagaaag attattagtt
      961 atgaagattt tgctaaatgt ataccaatgg gattcaaaac gatggttcca gcgatgatta
     1021 tacttacttt agcatggact ttaggggatt tagtgtcaga taaattacaa gctggggtct
     1081 ttgtttataa tttaatgcag ggagctagta tctctacagc aattttaccg gtttgtttat
     1141 ttttagttgg aacaggtcta gcatttgcaa cggggacttc atggggaact tttggaattt
     1201 taattcctat cgcgacttcg attttccccg aaggttcaca aatgcttgta attgcgattg
     1261 catctatttt agcaggggca gtctgtggcg atcatatttc accaatttct gatacgacga
     1321 tcatggcttc ggccggagct caatgtaatc atgttaatca cgtcacttct cagatacctt
     1381 acgcaatgct agttgctgga gcttgtgttg ttggatattt agttgctggt tttactacaa
     1441 atgttgtatt gacatttgtg attgcaataa ttgccttatt catatttatt tttataacca
     1501 aatattttta taattttaaa gcaaagaagg actaaatgtc ctttttcttt atactttatt
     1561 ttataattat attaaataac ggttatattt gatataatga aaatagagtg tgaccagaaa
     1621 ggtggtgtta aaatggaaga aagtcataat ttttatttaa acgattatac tgaatttgtt
     1681 aaacaggcga ctgatattat cgaaagtaat aagaatagta aaaattatgt aattatgtac
     1741 gctgatttaa ctaattttca attagttaat gacttttatg gttttattga aggggatcgt
     1801 tttcttttag aatttgctaa atttttaagt atgtctccac ggacaatttt atgtggacgt
     1861 gttttttcag atcatttttt aagactatct ttatttgaag acaattgtga tattgaatct
     1921 gtaacaagga attatgaaca aaaattagaa acttttattt catcgcaaga agaacatcac
     1981 ccggattcaa agattgctgt agcagcagga ttgtgtccag ttaaattaga agaaaaagct
     2041 atcattaaag caattgatca agctaatata gcacgaaaag aagctaaaaa agataatcat
     2101 tgtaaactag tttggtttaa tgaagatatg cagaaattta ttaataaacg taaaatatta
     2161 gaaattgaga ttcaaaatgc tttacgtaat aaattattta cattctatct tcaacctaaa
     2221 gttaatatta atacgggaaa aattgttgga agtgaagcat tagcccgcct tattaaagat
     2281 aataaagtta tttatcctga tcaatttatt gaaataatgg agaaagtatg caggtatctt
     2341 cgagaaagaa ttgatcaagg caaaaaacta attcctgtat caatgaatat gtcaagaatg
     2401 catgtttatg aaactggttt tgttgctagg gttaataaaa ttgttaataa atataatatt
     2461 cctccatatt tattggaatt tgaattaaca gaaacggtta ttttgaagaa tttaaccaag
     2521 gtgagagaag tagctgatga attacgaagt tttggttatc gtgtttcgat tgatgattat
     2581 ggaacaggtt actcagggat gaatatttgg caagatctaa attttgatat tgttaaattg
     2641 gatcgttcat atataactaa aagagagtca aataataatc gcaataatat tattattcct
     2701 gcaatcgttg atatttgtaa taagttacag acaacaatta tttgtgaagg agttgaaaca
     2761 ttggatcaat gtttatatat gaaacgtttt ggatgtaatg tagttcaagg ttattatttt
     2821 tcaaagccag ttagcacagt agaatttgaa aaaatgattg atgaaacgga cggtaaattt
     2881 aatttaccat ggaataaaga tgattttaaa tatacttctg ttgacaatat ttctcaaagt
     2941 gaaatcaaag ctattactaa ttctatcttt aattttattc ctggtggggt ggctggattc
     3001 agttatgaat tagaactact atttgtaagt gatagtttgg ttgaattaag tggttatagt
     3061 cgagaagaat tgctaagtgg aggaattgaa tggcctaaaa agttgtttta tagcgaagaa
     3121 gaatttaaac atgttttcta tcataatgga ttagctgatc gttcttctaa aaatttagtt
     3181 gagtttaaaa ttaaagctaa aaatggtaaa accgtgtata ttaatatgta tgggggatta
     3241 gttgatagtc cagagtgggg ttcatattat ttatgttgtt tttatgatgt tacaaatgaa
     3301 aaagtgaatg aattaaaatc acatgaatta caagaaaata taaatagttt attaaaaaat
     3361 attaacggtg gaattggtaa aatgacactt gatggcaaag gaacaatttt atttgcatca
     3421 gatggttttt ataaaacgat tgggtatact cgtactgatt ttaatcaacc gccaatcaat
     3481 aatgatttaa gtcagatagt tagtaaagaa gcttattttt tatgtaatca tgtaattgaa
     3541 cagattaaag ctggtaaaat cgattatttt gaagttgaag taaattccaa aactgcacaa
     3601 aagatttggc taacgatcta tttttctaat attcacagag aaaatggaaa attagtagct
     3661 gatgtttttt gtatcgatag cactgttgat catcaacaaa tgatgctaga acaacactgt
     3721 aatgagatgg aagataattt aaatattgtt attgaaaata ccccagggga tattgtcgtt
     3781 attaaaataa aagatgatca tattaccact aaattcctta gtcatggatt atctaaaata
     3841 tttgattttg aacaatctga gttaacagat atactgaaac ataataaagg atttgattta
     3901 gtttatgaag atgatcgaga agttttagct aaacaattta tagaattatc taaacaaaaa
     3961 agttgtgtaa attttgatta tcgctcatat tataaaaatg gtaaagtcag ttggcataat
     4021 atcaatgcaa acttttatcg tcaagaagaa gatggaacaa tcatttatca tggaattatc
     4081 actaatatta atactcttaa agaacaacaa gaacgtgcgg aaaaattaaa agatcgttat
     4141 aattttattt tatcagtgtt ggctgttgat gtttggaatt ttgatgttag aaataatcgg
     4201 atggatagtt tatatatgga tgataaattt agtattgatt tacctaaagg agcaaaaata
     4261 gttcctgaaa aattattaga agatgcgctt attttacctg aagatcagaa taaatattta
     4321 aatatgctca cttcaataag agcgggacag aaaacagccc atgataaatt acattgtcga
     4381 attaaaacgg gtgaatataa tttatttgat cttatgataa gagtaaaaga tactaattca
     4441 gatatggcta taattatggc taaaaatttt gatcaatttt ttaataaaaa agaataggaa
     4501 aaaataaggg ctaaattatt taggccctta ttttaataat gaacggttgt taactaatga
     4561 tttttaatga actttgatga ttgttttagc tattaatatc acgtatgatg ttgttgtggg
     4621 ggtgagaaga tgcagataaa aaaagaagat ttaaagaatg atattattga agctgctaaa
     4681 gtggaatttt tgcatcatgg ctacgaaggg gcttcaatga gaataattgc tagtaaatca
     4741 cataccacct tgggaaattt atataattat tttactaata aagaagaatt attaggagct
     4801 gtattagagc catcaattaa gtcattaaat aagttagttg aggttcatct aaatgaagaa
     4861 attcaagttc attcactaga agaggttgat gaagcacttg atcaatttgg agattttttt
     4921 gaagaaagtg attttcgata tataatggat gaacgattaa ttatcctttt tgaattaaaa
     4981 acgacggaat ataaagaaaa aagggattgg tttcttttaa aatttaagca acatatggca
     5041 tggcatctta atattgaaga tatcaattcg ccgtatattg atattattac aaatatgttt
     5101 attgattgta ttaagcatgt attagtaagt catgataatc ttgaaatgca aaaacaagaa
     5161 tttttaaaag ttttcaaaat gttgtgtaca ggcattgttg ttaacgaaga gaaataaaaa
     5221 cttgcagtga tgattcattg tcttttgaat acatgttttt attatgcaaa aaaggagaaa
     5281 atatgattaa atttgaagaa gtatcaaaga tttatcaaaa tggaaataaa aaaatttatg
     5341 ctttaaataa ggtttctttt acgattaata aaggtgaatt tgtcattatc ttaggaccga
     5401 gtggggcagg gaaaagtact ttattaaata ttttaggggg aattgatcgt cttgatgaag
     5461 gaaaaataat tattgataat gaagtaattt cgaataaaaa tgaaaatgaa ttatcagatt
     5521 atcgagctca tcaagtagga tttatatttc aattttataa tttaattcca acactgacgg
     5581 tttatgaaaa tgtagccttg atgaaggaac taaaaaaaga tattatgcca gttgataacg
     5641 ttttagatca agtaggacta ttagtgcata agaaaaaatt ttctcatcag ttatctgggg
     5701 gagaacaaca gcgagtttca attgctcgag cagtggtcaa aaatccagag atttttttat
     5761 gtgatgagcc gacgggagct ttagatagtg atacgggagc aaaagtattg aagttattac
     5821 atgatatgtg taaagaatat cataaaacaa ctatcattgt tacccacaat actaatttgc
     5881 aagctatcgc tgacaaagta attcgagtaa aaaatggtca aatcaataca attacaacaa
     5941 attctcatcc tcttgatgtg atggaggtgg aatggtaaat gttgttaaaa aaaatgattc
     6001 gagatttatg gaaaaataag gttcagtttg gagctatatt tattatgatg tttttaggtg
     6061 tttttatctt ttcaggtata tctagtgaat atcatggact tgaagtagct ttaaataact
     6121 atattaaaga gaataattta gctgatgctt ggctttacca aaaggaattt agtgattcga
     6181 ctcttaatga actaaaaaaa cagactgatt atgaacaaag aatgtatgtc tctacaagca
     6241 ataaagcggt tgataatagt aatattgatc tctatattac tgatagtaat aaaatttcaa
     6301 ctcttaaagt cgttaatggt aaatcattta ataatcaaga tgatggtatc tggcttgatg
     6361 caatgtatgc taagaaaaat ggttatcagt taaatgacca aataacttta aaatatcaga
     6421 attttgaatt aagtaagaaa attattggtt tagtttattc ccctgaaaat atctatctgg
     6481 ctaaagaaga gcaactgaca ccaaatcgtc aaaaatatgg ttttgcatac gtaaataata
     6541 atagttttcc taacacttta taccatccaa atcaactagt cctttcctca aaaaaagaca
     6601 ttagtaaaat agttgatcaa ttattagctg ataatgaata taagctaatt ctccaaaaag
     6661 atcattctag tgtaaatatg ttgactggag agattgctca gcatcgctcg ataggtttgg
     6721 ttttttcatt agctttttta tttattgctg ttttaattac gattacaaca atgcatcggg
     6781 ttttgc
//
LOCUS       DAOMTA010000049         6743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_171_length_6743_cov_2.118870, whole genome shotgun sequence.
ACCESSION   DAOMTA010000049 DAOMTA010000000
VERSION     DAOMTA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_6743_cov_2.118870"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>270
                     /locus_tag="PLK68_02280"
     CDS             153..>270
                     /locus_tag="PLK68_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM89268.1"
                     /translation="MKKLFSSDKLYKIFIYVALITLAVSIIVPVAWVFMASIK"
     assembly_gap    271..280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <281..1030
                     /locus_tag="PLK68_02285"
     CDS             <281..1030
                     /locus_tag="PLK68_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM89269.1"
                     /translation="EFYGNPWSIPAGIYLQNFVNAFEQANMGEYLLNSILTTAMALVI
                     LLVVSLPAAYVLARYNFKGRRFFNTLFMAGLFINVNYIVVPIFLMLLDWDDFIYELLG
                     GNFFLNNIFVLAVVYAATAIPFTVYLLSGYFKTLPKAYEEAALIDGCGYYKTMVRVMI
                     PMAKPSIITVILFNFLAFWNEYIIALTLLPSTSKTLPVGLLNLMQATRGKAEYGMMYA
                     GLVVVMLPTLILYIIVQKKLTQGMTLGGLKD"
     gene            1042..1209
                     /locus_tag="PLK68_02290"
     CDS             1042..1209
                     /locus_tag="PLK68_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89270.1"
                     /translation="MDGKTKIIIMVVVFIVSMALIFIGQKNVGYTGLATELVGLAGLI
                     GVIYTYNKGYK"
     gene            1222..2340
                     /gene="ugpC"
                     /locus_tag="PLK68_02295"
     CDS             1222..2340
                     /gene="ugpC"
                     /locus_tag="PLK68_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRM89271.1"
                     /translation="MANLSLKHIDKIYDNNVQAVFDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISAGELYIDGKLMNDVAPKNRDIAMVFQSYALYPHMSVYDNMAFGLK
                     IAKKPKDEIDQKVRKAAKALDIEQYLDRKPKALSGGQRQRVALGRAIVREPKVFLMDE
                     PLSNLDAKLRVQMRTEISKLYQQLGTTFIYVTHDQTEAMTMGTRIVVMKDGRIQQVAS
                     PAYLYNHPVNKFVAGFIGSPQMNFLNGKINEKDGAIIIELSDVSLKVESTRAKQLREK
                     GYLGKTVIMGIRPEDISVDNDYINEHQEDCFEGKAEVVEFMGSESYIHMVKDEKPFIV
                     KAPGGTTLTNGVIGRFAYKMDKMHFFDIDSEEAIIEGE"
     gene            2343..>3343
                     /locus_tag="PLK68_02300"
     CDS             2343..>3343
                     /locus_tag="PLK68_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase N-terminal domain-containing protein"
                     /protein_id="HRM89272.1"
                     /translation="MEKYGRLTLPTDLDVIDQTIELKNKLGADAIRDCDGTDMPQELM
                     NLDAKIYATYYTTRKDNEWAEANPDEIQQEYLITDRYTARDTSLTIELMKGFHTQQLK
                     VNDIDDPKRWWEVIDRTTGEVVAVDQWEFNKEVGAVTISTIPYHEYTVSFLAFLIWDP
                     VHMYNFITNDWKDAPHQLTFDVRQPKTKEFVKDKLRKFCEENKHVDVIRFTTFFHQFT
                     LTFDDQKREKFVEWFGYSASVSPYILEQFEKWAGYKWRAEYIVDQGYHNSLFRVPSKQ
                     FKDFIEFQQIEVCKLAKELVDIVHGYGKEAMMFLGDHWIGTEPYGKYFETIGLDAVVG
                     "
     assembly_gap    3344..3375
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3376..4519
                     /gene="gnpA"
                     /locus_tag="PLK68_02305"
     CDS             <3376..4519
                     /gene="gnpA"
                     /locus_tag="PLK68_02305"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HRM89273.1"
                     /translation="NGVTMRMISDIKGVRYTEGRFLPYFFPDVFCEGGDPIKEAKDNW
                     LQARRAILRSPLDRIGYGGYLKLAVEWPGFVDTITQVVDEFRQIHDLTKETKAYTSPF
                     KVAILNCWGSIRRWMNNQVHHAIWYREIYSYVGIIECLSGMPIDVEFINFDDIKKEIN
                     PDIKVIINAGTAYTSWSGAENWIDEEVLTTIRKWIDNGGGFIGVGEPTAYQYQGKFFQ
                     LSDVLGVDKEVGFTLSHDKYNTVDPNHFIVEDIEGEIDFGEGMASIYGHGENYQIIRQ
                     HKEFAQLVTNTYGAGRSVYLAGLPYSPQNCRLLLRAIYWAAAKEDEMKKYYVDNVNAE
                     VAAFEAVGKIVVINNSLDALDTHLYVEGNLREKLRLAPMEMRWLDI"
     gene            4570..5493
                     /locus_tag="PLK68_02310"
     CDS             4570..5493
                     /locus_tag="PLK68_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM89274.1"
                     /translation="MNKPVLVIMAAGMGSRYGGLKQIDPIDEDGHIIIDFSIYDARRA
                     GFETVIFIIKKENEEEFKEVIGDRMEQIMNVKYVYQDLANIPAEFSLPAGRVKPWGTA
                     HAIWSCKDLIDGPFAVINADDYYGVTAYKQIYDFLLNHPDGVKYNYAMVGYYIENTLT
                     ENGHVARGVCQVNDKQLLVDIHERTQIVRNGAGAKYTEDDGQSWVELPKGTIVSMNLW
                     GYNKSILLEIEKGINSFFEIGLKENPLKCEYFIPAVVSKLLDQNKVEVTVLESQERWY
                     GVTYREDKPIIQMAIKELKDAGVYPKHLWKE"
     gene            5500..6591
                     /locus_tag="PLK68_02315"
     CDS             5500..6591
                     /locus_tag="PLK68_02315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRM89275.1"
                     /translation="MSEELLNEVVEAFSFEGVLKKKKPYGSGHINDTFLLTFDIERMG
                     MIDIILQRMNTAVFTKPIELMENIANVTRFLRDKIIASEGDPERETLNIIYTLDHKPY
                     YVDSAGGYWRAYKFITCATSYDQVESAEDFYQSALAFGHFQSLLADYPAATLHETIKG
                     FHDTKARFERFKEVVANDICNRAKDVQTEIEFVLAREDVANVLTNCNLPIRVTHNDTK
                     LNNVMIDDKTHKGICVIDLDTVMPGLAVNDFGDSIRFGASTGAEDEIDLDKIECDMAL
                     FEIYAKGFIKGCDGKLTKEEIKALPIGAKVMTFECGMRFLTDYLEGDIYFKIHRKNHN
                     LDRCRTQFKLVKDMESKWEMMNKIVEKYM"
BASE COUNT         2288 a          780 c         1451 g         2182 t
ORIGIN      
        1 agttatttat ataaacaagc ttataacaac gcatcatatg gatatggaat ggcgtttgcc
       61 gcagtagttt ttattttttc atttattttg agttttatta ttaatcgtgc tacagagcgt
      121 gatacgttag agttatagaa aggagcagga aaatgaaaaa gttatttagc agtgataaac
      181 tttataaaat atttatttat gtagctttga ttactcttgc ggtttctatt attgtaccgg
      241 tagcatgggt atttatggct tcaatcaaag nnnnnnnnnn gagttttatg gaaatccctg
      301 gtctattccg gcaggaatct atttgcaaaa ctttgttaat gcatttgaac aagcgaatat
      361 gggagaatat ttacttaatt ctattttaac aacagcgatg gcactggtga ttttattagt
      421 cgtttcatta ccagcggcgt atgtattggc gcgttataat tttaagggaa gaagattttt
      481 taacacctta tttatggcag ggttatttat taatgtaaat tatattgttg tacctatctt
      541 tttaatgtta ttagattggg atgattttat ttatgaatta ctcggaggaa atttcttttt
      601 aaataatatt tttgttttag ctgttgtata cgcagcgaca gctattccgt ttaccgttta
      661 tttattaagt ggttatttta agactttacc aaaggcttat gaagaagcag cgttaataga
      721 tggatgtgga tattataaaa caatggttag agtaatgatt cccatggcaa aaccaagtat
      781 tattactgta attttattta attttttagc attttggaat gaatatatta ttgccttgac
      841 attattacca agtacttcaa aaacattacc tgtaggattg ttgaatttga tgcaggcaac
      901 ccgggggaag gctgagtatg gtatgatgta tgctggatta gtagtagtta tgctgccaac
      961 gttgattcta tatattatag ttcaaaagaa acttacacaa ggaatgacct tgggtggatt
     1021 gaaagattag aggagaaaac tatggatgga aaaactaaaa taattatcat ggttgttgta
     1081 tttatagttt caatggcttt gatttttatt ggacaaaaaa atgttggtta tacaggatta
     1141 gcgactgaac tggtgggctt agctggattg atcggagtaa tttacacata taataaaggc
     1201 tataagtaaa ggagaaacaa gatggcgaat ttaagcttaa aacatattga taagatatat
     1261 gataataatg ttcaagctgt atttgatttt aatctagaaa tcgcagataa ggaatttatt
     1321 gtttttgttg gtccttctgg ttgtgggaag agtactacac ttagaatgat tgcgggatta
     1381 gaagaaattt cagcaggaga gctatatata gatggaaaat tgatgaatga tgtggctcca
     1441 aaaaatcgag atattgcaat ggtgtttcaa tcatatgcat tatatcctca tatgagtgta
     1501 tatgataaca tggcttttgg tttaaaaata gcaaaaaagc caaaagatga aattgatcaa
     1561 aaggttcgta aagcagctaa agctttagat atagaacaat atttagatcg taagcctaaa
     1621 gctttatccg gtggacaaag acaacgggta gcattagggc gagcaatcgt tcgagaacca
     1681 aaagtatttt taatggatga gccattgagt aatttagatg ctaaattaag agttcagatg
     1741 cgaacagaga tttcaaaatt atatcagcaa ttaggaacaa catttattta tgtgactcac
     1801 gatcaaactg aagcgatgac gatgggaaca agaattgttg tgatgaaaga tggtcggatt
     1861 caacaagtag catcaccagc atatttatac aatcatcccg tcaataaatt tgttgctggt
     1921 tttattggga gtccacaaat gaatttttta aatggtaaaa ttaatgaaaa ggacggtgct
     1981 attattattg aactgagtga tgttagttta aaggttgaat caactagagc taagcagctt
     2041 agagaaaaag gatatttagg caagacggta ataatgggaa taaggccgga agatatcagt
     2101 gttgataatg actacattaa tgagcatcag gaggattgtt ttgaggggaa agcagaagta
     2161 gttgaattta tgggttctga aagttatatc catatggtta aagatgaaaa accgtttatt
     2221 gttaaagccc ctggcggaac aactctgaca aatggggtaa ttgggagatt tgcttataag
     2281 atggacaaaa tgcatttctt tgacatagac agtgaagaag ctataattga aggagagtag
     2341 acatggaaaa atacgggaga ttaacgttac ctactgattt agatgtaatt gaccaaacga
     2401 ttgaattaaa aaataaactt ggggctgatg caattcgaga ttgtgatggg accgacatgc
     2461 ctcaagaatt aatgaatttg gatgctaaaa tatatgcaac atattatact acaagaaaag
     2521 ataatgagtg ggctgaagct aatccagacg agatccaaca agaatatttg ataacggacc
     2581 gttatactgc tcgtgatact agtttaacga ttgagttaat gaaaggtttt catactcagc
     2641 agctgaaagt taatgatatt gatgatccta aacgctggtg ggaagtaatt gatcgtacta
     2701 ctggtgaagt agtagcagtt gatcaatggg aatttaataa agaagttgga gcagtaacaa
     2761 tttcgactat accttatcat gaatatacag ttagtttttt ggcattttta atttgggatc
     2821 ctgttcatat gtataatttt attacaaatg attggaaaga tgcgccgcat caattaacct
     2881 ttgatgtccg tcaacctaag accaaggaat ttgttaaaga taaattacgg aaattttgtg
     2941 aagaaaataa acatgttgat gtaattcgtt tcaccacttt ttttcatcaa tttactttaa
     3001 catttgatga tcaaaaaaga gaaaaatttg tagaatggtt tggatatagt gcaagcgtta
     3061 gtccttacat tttagagcaa tttgaaaaat gggcaggtta taaatggcgc gctgaatata
     3121 ttgttgatca aggatatcat aattcattat ttagagtacc atcaaaacaa tttaaagatt
     3181 ttattgagtt ccaacaaatt gaggtttgta aattagctaa ggaattagtt gatattgttc
     3241 atggttatgg taaagaggct atgatgtttt tgggtgatca ttggattgga acagaaccgt
     3301 atggtaaata ttttgaaaca attggtttgg atgctgtagt tggnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnaaatg gtgtaacgat gcgaatgatt tccgatatta aaggcgtacg
     3421 ttatacagaa gggcgttttt taccatattt cttccctgat gttttctgtg agggtggtga
     3481 tccgattaaa gaagcaaaag ataactggtt acaagcaaga agagctattt taagaagtcc
     3541 gctagatcga atcggttatg gaggatattt gaaattggca gtggaatggc cgggatttgt
     3601 tgatacaatt acccaagtag ttgatgaatt tagacaaatt catgatttaa caaaagaaac
     3661 aaaagcctat acctctccat ttaaagtggc aatactaaac tgttggggaa gtattcgacg
     3721 ttggatgaat aatcaggttc atcatgctat ctggtatcgt gaaatttatt cttatgtagg
     3781 gataattgaa tgcttaagcg ggatgccgat cgatgttgag tttattaatt ttgatgatat
     3841 aaaaaaagaa attaatcctg atattaaagt aattattaat gccggtacgg cttacacatc
     3901 atggtcagga gcagaaaact ggattgatga agaggttctt acaaccatta gaaaatggat
     3961 agataatggt ggcggtttta tcggggttgg agaaccaacc gcttatcaat atcagggaaa
     4021 attctttcaa ttaagtgatg ttcttggtgt tgataaggaa gttggcttta cattaagtca
     4081 tgataaatac aatacagttg atcctaatca ttttattgtt gaagatattg aaggagaaat
     4141 tgattttggt gaaggaatgg ctagcattta tggacatggt gaaaactatc aaattattcg
     4201 tcaacataaa gagtttgctc aattagttac aaatacttac ggtgctgggc gtagtgttta
     4261 tttagctgga cttccttatt caccgcaaaa ttgtcgttta ttattaagag caatctattg
     4321 ggcggcagct aaagaagatg aaatgaaaaa atactatgta gataatgtca atgctgaagt
     4381 agctgctttt gaagcggtag gaaagatcgt agttattaat aactcactag atgctctgga
     4441 tacccatcta tatgtagaag gaaatttacg agaaaaatta agattagcac caatggaaat
     4501 gcgttggctt gatatttaat tgaaagactc attttctttg aaagtgggtc ttttaaaagg
     4561 aggataaaaa tgaataaacc agtattagta attatggcag caggtatggg gagtcgttat
     4621 ggcggattaa agcagattga tccaattgat gaggatggtc atataattat tgatttttcg
     4681 atttatgatg ctcgacgagc aggttttgaa actgttatat ttattataaa aaaggaaaac
     4741 gaagaagagt ttaaagaagt tattggagat agaatggagc aaatcatgaa tgttaaatac
     4801 gtatatcagg atttggctaa tattccagcg gaattttcat taccagctgg tcgtgtgaaa
     4861 ccatggggaa ctgctcatgc gatttggagt tgtaaagatt taatagatgg gccatttgca
     4921 gtgattaatg ctgatgatta ttacggagtt acagcgtata aacaaatcta tgattttcta
     4981 ttaaatcatc ccgatggggt aaaatataat tatgcaatgg taggttatta tattgaaaat
     5041 acattaactg aaaatggtca tgtggctcgg ggtgtttgtc aggtaaatga taaacaactg
     5101 ctggttgata ttcatgaaag aacacaaatt gttcgaaatg gtgcaggtgc aaagtatact
     5161 gaggatgatg gtcaaagttg ggttgaactg cctaaaggaa caattgtttc aatgaatctt
     5221 tggggatata ataagagtat cttattagag atagaaaaag gaataaatag tttttttgaa
     5281 atcggtttaa aagaaaatcc gttaaagtgt gagtatttta ttcctgcagt tgtaagtaaa
     5341 ttacttgatc aaaataaggt agaagttacg gttctagagt ctcaagaacg ctggtacggt
     5401 gtcacttatc gtgaggataa accaataatt caaatggcga ttaaagaatt aaaagatgca
     5461 ggtgtttatc ctaagcatct ttggaaggag tgatcaatag tgagtgaaga gttattaaac
     5521 gaagtagttg aagcattttc atttgaaggt gtgttgaaga aaaaaaagcc ttatggaagc
     5581 ggacatatca atgatacatt tttattgact tttgatattg aaagaatggg aatgattgat
     5641 atcattttgc aacgaatgaa tactgcggtt tttactaaac cgattgaatt gatggaaaat
     5701 attgctaacg taacaagatt tttacgagat aaaattattg ctagtgaggg tgatcccgaa
     5761 agagaaacac tcaatattat ttatactttg gatcataaac catattatgt agattcagct
     5821 ggtggatatt ggcgagcata caaatttatt acttgtgcaa ctagttatga tcaagttgag
     5881 agtgctgaag atttttatca aagtgcatta gcttttggac acttccagag ccttttggct
     5941 gattatccgg cagcaacact tcatgagaca atcaaaggtt ttcatgatac taaagctcgc
     6001 tttgaacgtt ttaaagaagt agttgccaat gatatttgta atcgtgctaa agatgttcaa
     6061 acagagattg aatttgtttt agctcgtgaa gatgtggcaa atgtattaac aaattgtaat
     6121 ttgccaatac gggtcactca taatgatacg aagttaaata atgttatgat tgatgataaa
     6181 actcataaag gtatttgtgt gattgattta gatactgtta tgccaggttt agcagtcaat
     6241 gactttggtg attcgattcg atttggagcc agtacagggg cagaagatga aattgattta
     6301 gataaaatcg aatgtgatat ggcgttgttt gaaatttatg ctaaaggttt tatcaaaggt
     6361 tgtgatggta aattaacgaa agaggaaatc aaagcattac cgattggggc taaggttatg
     6421 acatttgaat gtggaatgcg ttttttaact gattatttgg agggtgatat ttatttcaaa
     6481 attcatcgca agaatcataa tcttgatcgt tgtagaactc aatttaagct tgttaaagat
     6541 atggaatcaa aatgggaaat gatgaataaa atcgttgaaa aatatatgtg attatattaa
     6601 agaaggtaaa tttttagcaa tatatgggaa taatttgtaa gcgctaaatt ttatgtaaaa
     6661 agttctttaa aaccaagggg ttaggtgtta taattgattt atagaaattt taaggaggag
     6721 tcataaaatg gcagaaaaat tat
//
LOCUS       DAOMTA010000050         6662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_177_length_6662_cov_2.677161, whole genome shotgun sequence.
ACCESSION   DAOMTA010000050 DAOMTA010000000
VERSION     DAOMTA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_6662_cov_2.677161"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLK68_02320"
     CDS             <1..317
                     /locus_tag="PLK68_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89276.1"
                     /translation="LVNDAIKYIHDHLHEPIKLTAMALQLGVSTSMLYKSFIAILAIP
                     PLTYIHQQKVLYAQRMLAHGKSVTMIANDLGYSSAYHLSKTFKQITGVSPREYKKNIK
                     LL"
     gene            403..2358
                     /locus_tag="PLK68_02325"
     CDS             403..2358
                     /locus_tag="PLK68_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89277.1"
                     /translation="MNLENKYQKLFQPIKINQLEVKNRIFMSPMSTNFATKDGYVTDE
                     MIYYYSRRAKGGVGLIVTEVTMIEPTYKYIAHTLSIQDDSYLEGWQKLANEIHKYDSK
                     VVAQLLHPAYMAIPFPETPQLVGPSEVGPYYAKTPPRPLTKEEISIIVEQFGDGALRL
                     KKAGIDGVEIHAAHAHALLGGFLSPLYNKRIDEYGGDITHRIRLLLEVIANVRKKCGR
                     DFVIIVRISGDDYEAGGQSLHEGCYIAKRLEAAQVDMIHVSGGTTIHRGSSIAPPGTP
                     QASHIDSAREIKKCVNIPVSTVGRLNEPWILEEVLERDLADVCMVGRSLLCDPDFVNK
                     IKNGQEDDIRPCIGCLGCLSSTMLKDHVECGINPSLTSENEETVTPATISQNILVIGG
                     GPAGLEAAYILSKRGHKVTLAERHHTLGGAMIVAGYPIAKQHFAQTTKYFIKRVIDCN
                     IKIELNTIVDQAYLSQHHYDHIIVATGAKPLRLDIFKNHPHTGTGQDVLLGKMWTGKN
                     IVVVGGGSVGCEVADFVAPLVNDRHPNNKKVTVIEMKSNIIMDDTSPSRSILVQRMAD
                     KGVNIITDSKVVKVTPTTLEYQKDGNTITIENVDTIIEATGYYADHELEKTLDQMQLS
                     YNVLGDAATPGKIKNAISDAYHLCKNI"
     gene            2375..>3448
                     /locus_tag="PLK68_02330"
     CDS             2375..>3448
                     /locus_tag="PLK68_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRM89278.1"
                     /translation="MNLLKPIKVGKITLKNRIMFPPMTTGYEERDGSIGEQSFNFYKR
                     LAQGDVAYIVLGDVAPVNTVSPTPKLFHDGQIEAFKKLSDALHEYDCKLGIQIFHPEY
                     DVDALAELFRKGDMQAARAKLHHDMLHYIDEVTEAQLTAIIDKIGACVKRAAAAGVDV
                     IEVHGDRLVGSLCSTILNHRNDNYGGSFENRIRFALEVVKVIKENAPDICIDYKLPII
                     TENPLRGKGGLKIDEAIKLATILEAHGVDMIHVGQANHTGNMNDTIPAMGTQPYCFMN
                     KYTKQIKDVVTIPVSSVGRILTPQNGESLIDNGICDIIGLGRSLLADPDYVKKLKNNE
                     AARIRHCMMCNKGCTDAIQNRQFL"
     assembly_gap    3449..3458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3459..4378
                     /locus_tag="PLK68_02335"
     CDS             <3459..4378
                     /locus_tag="PLK68_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM89279.1"
                     /translation="CIRECLNCNKGCVDAIQNRQYISCVLNAENGYEYKRIITPAATK
                     KKVVVIGGGPAGLEAARVAKTKGHDVILFEQDTRLGGQLNIAAIPPRKAEMNRAINYL
                     SNEMKILNVDLRLGKKATSTDILNEHPDSVIIAVGANNASLPIPGSDLTHVLDAWKVL
                     NYEQFPSGRVVIIGGGLVGAETAEFLAQMGLNVTVIEMLEEIAKEESSTVKPVMFEDF
                     EKHQVKLLTKTKVIEIKNDCIKAANGDGELTIPCDYVILATGAKPNPFDVSELTAHNI
                     GIHLVGDCNEKAADINNAITQGYLAANSI"
     gene            complement(4457..5392)
                     /locus_tag="PLK68_02340"
     CDS             complement(4457..5392)
                     /locus_tag="PLK68_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89280.1"
                     /translation="MSNVKRLINASKSEINKMGPLELKESIYKSEGRVIMGQHLIFAG
                     PGTVMGVTNAELLCSFGADMVMLNTIDLDDFENNPGLCGLTIKELKEKCNCPIGVYLG
                     CPKKGEKRSDEKTLYRLAGMLATKEHILKAVALGVDFIVLGGNPGSGTSIEDVIEATK
                     LVKEICGDDIFLFAGKWEDGIEEKVLGDPLAAYDTKDVIKRLIDAGADCIDLPAPGTR
                     AGITVDMIRELVEYIHRYKPGTLAMSFLNSSVECADEDTIRQVALLMKQTGCDIHAIG
                     DGGFGGCTWPENIYQLSVSIKGKNYTWFRMASNSR"
     gene            complement(5408..>6662)
                     /locus_tag="PLK68_02345"
     CDS             complement(5408..>6662)
                     /locus_tag="PLK68_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89281.1"
                     /translation="ISGAFMKVMPELLGGAVFSLFQGLPLGDWYTNFLSSTGISDALA
                     VGVAVCNLTALYFIFALGYNLGEKFNVNPFQTAIVSLLSLLLVTPFSYNNVDSTNGAV
                     TLIKNVIPVDNLGASGIFTAMLCGIIGAYVFAFAVNHNWKIKLPDSVPEMVSKPFEAV
                     VPAFLVSALFLLVRSGFAVTSYGNIQNFIYTMIQAPLVSLGNSFPAMLISMLILLLLW
                     WVGIHGTSVVLSVMMVIWMEPAITNLNNYMAGEPVTLVTTYMFFFVFCQFIGGPGCLF
                     GLTCDMAIFAKSERFKAFGKVCFVPGMFNIVEPVIFGFPIVLNPIMFIPFILTPLVFM
                     IIGYLLMVTGVVNIPALMLSVMTIPGPIAGFILGGGISLGIMIVIMCLLSCIIYYPFF
                     KICDNQALEAEKKSNQEKTLEENKI"
BASE COUNT         2305 a         1208 c         1107 g         2032 t
ORIGIN      
        1 aattagttaa tgatgccatt aagtatattc atgaccatct tcatgaacca attaaactaa
       61 ccgcaatggc tttacaacta ggtgtttcaa caagtatgtt atataaatct tttattgcaa
      121 ttttagcaat tcctccacta acttatattc atcaacaaaa agttctctat gctcaaagaa
      181 tgctggctca tggtaaaagt gttacaatga ttgcaaatga cctgggatat tcatcagcct
      241 atcatctatc taaaactttt aaacaaatca caggggtcag tccacgtgaa tataagaaaa
      301 atattaagtt attataagca tatacgaaaa tgtatatgtt ttttaattga tacaatatgt
      361 tttatatata tgtaagtgct atcatatctt tataagggag gtatgaattt ggaaaataaa
      421 tatcaaaaac tctttcaacc aataaaaatt aatcaactgg aagtaaaaaa tcgaattttt
      481 atgtcgccaa tgtcaacaaa ctttgcaaca aaagatgggt atgttactga tgaaatgatc
      541 tattactata gcagaagagc aaaaggtggc gttggtctga ttgtaactga agtaacaatg
      601 attgagccaa cctacaaata tattgctcat actttatcaa ttcaagatga ttcctatctt
      661 gaaggatggc aaaaattagc taatgaaatt cataagtatg atagtaaggt cgtagcccaa
      721 ctactgcacc cagcatatat ggcaattcct ttcccggaaa caccacaatt agttggtcca
      781 agtgaggttg gtccatacta tgctaaaact cctccgcgac ctttgacaaa agaagaaatc
      841 agtataattg ttgaacaatt tggtgatggt gccttacggt taaaaaaagc aggaatagat
      901 ggtgttgaaa ttcatgctgc acacgcacat gcattactag gaggattttt atctccactc
      961 tataataaac gaattgatga atacggtggt gacataactc atcgcattcg tctactttta
     1021 gaagtaattg caaatgttcg aaaaaaatgt gggcgtgatt tcgttattat tgttcgtatc
     1081 agcggtgatg attatgaagc cggcggacaa tcattacacg aaggatgtta tattgctaaa
     1141 agattagaag ctgctcaagt tgatatgatc catgtttctg ggggaactac tatccaccgc
     1201 ggcagctcaa ttgcaccccc tggaacacca caagcgagtc atattgacag cgcacgagaa
     1261 ataaaaaaat gtgttaatat ccctgtttca accgtaggac gtttgaatga accttggatt
     1321 ctcgaagaag ttttagaaag agatttagca gacgtatgta tggtaggtcg ctcactctta
     1381 tgtgatcctg attttgttaa taagatcaaa aatggtcaag aagatgatat tcgtccatgt
     1441 attggatgct tgggatgttt aagctcgacc atgttaaaag atcatgttga atgtggaatt
     1501 aatccatcat tgacaagtga gaatgaagaa actgtaacac ctgcgacaat atcccaaaat
     1561 atcctagtta tcggaggagg tccagctgga ttagaggcag cttatatttt atcaaagcgt
     1621 ggacacaaag ttacccttgc tgaacgtcat catacattag gcggcgcaat gatcgttgca
     1681 ggttatccta ttgctaaaca gcactttgct caaactacta aatatttcat taaacgagta
     1741 atcgattgta atatcaaaat tgaattaaac acgattgttg atcaagccta tttatcacag
     1801 catcattatg atcatatcat cgtcgcaacc ggagccaaac cattacgact tgatattttt
     1861 aaaaatcatc ctcatactgg taccggacaa gatgtgcttc ttggcaaaat gtggacaggt
     1921 aaaaatattg tcgttgttgg aggaggctct gttggctgtg aagttgctga ttttgttgcc
     1981 ccattagtta atgaccgcca tccaaataat aaaaaagtaa ctgttattga aatgaaatca
     2041 aatattatta tggatgatac ttcaccatca cgaagtattt tagttcaacg gatggctgat
     2101 aaaggcgtga acattattac tgatagtaaa gtcgttaaag ttacaccaac aactctcgaa
     2161 tatcaaaaag atggcaatac tattacaatt gaaaatgttg atacaatcat tgaagctact
     2221 ggttattatg ctgatcatga gttagaaaaa accctcgatc aaatgcaact tagctacaac
     2281 gtcttaggtg acgctgcaac cccaggaaaa attaaaaatg ctataagtga cgcatatcat
     2341 ttatgtaaaa atatttaatc aaaggagaaa atatatgaat ttactaaaac ctattaaagt
     2401 tggaaaaatt actttaaaaa accgaattat gtttccccca atgacaaccg ggtatgaaga
     2461 aagagatggt tcaatcggtg aacaaagttt taatttctat aaacgtttag cgcaaggcga
     2521 tgttgcttat attgttttag gtgatgttgc tccagtcaat actgtttccc ctacacctaa
     2581 actttttcat gacggtcaaa tcgaagcttt caaaaaatta agcgatgcct tacatgagta
     2641 tgattgcaaa ctagggattc aaatctttca tcctgaatat gatgttgatg ctctagcaga
     2701 attatttaga aaaggtgata tgcaggctgc acgtgctaaa ttacatcatg acatgctaca
     2761 ttatatcgat gaagtaactg aagcccagct tacagcaatt attgataaaa tcggcgcttg
     2821 tgttaagcgt gctgccgccg cgggggtgga tgttattgag gttcatggtg atcgtcttgt
     2881 cggttccctt tgctcaacca ttctaaatca ccgaaatgat aattatggtg gttcatttga
     2941 aaaccgtatt cgctttgctc tcgaagttgt caaagtaatt aaagaaaatg ctccagatat
     3001 ttgcatcgat tataaactgc caattatcac tgaaaatccg cttcgtggta aaggaggcct
     3061 aaaaattgat gaagctatta aattggctac tatcttagag gctcatggag ttgatatgat
     3121 ccatgttggt caagccaatc atactggaaa tatgaatgat accattcctg caatgggaac
     3181 tcaaccatac tgttttatga ataaatatac aaaacaaatt aaagatgttg ttacgattcc
     3241 agtgtcttct gttggacgaa tccttactcc ccaaaatggt gaatccttaa ttgataacgg
     3301 gatttgtgac attattggtt taggtcgttc attattagct gatcctgatt atgttaaaaa
     3361 attaaaaaat aatgaagctg ctcgaattcg tcattgtatg atgtgtaata aaggctgtac
     3421 cgatgcaatc caaaatagac aatttttann nnnnnnnnaa tgcattcgag aatgtttaaa
     3481 ctgtaataaa ggatgcgttg atgcaattca aaatagacaa tacatttcat gtgttttaaa
     3541 tgctgaaaat ggttatgaat acaaacgaat aattacccca gcagcaacaa agaaaaaagt
     3601 tgtagttatt ggagggggac cagcaggttt agaagctgct cgggtagcaa aaacaaaagg
     3661 acatgatgtt attttatttg aacaagacac tagattaggt ggtcaactta atattgcagc
     3721 gattccacca cgtaaagctg aaatgaatcg ggctatcaat tacttatcaa atgaaatgaa
     3781 aatattgaat gttgatttaa gattaggtaa aaaagcaact agtacagata tattaaacga
     3841 acatcctgat agtgttatca tcgctgttgg tgccaataat gcttccctac caattcctgg
     3901 tagtgattta actcatgtgt tagatgcttg gaaagttctt aactatgaac aattccctag
     3961 tggccgtgta gtgattattg gcggtggtct agtcggagcg gaaactgcag aatttttagc
     4021 acaaatgggc ttaaacgtta ctgtcataga aatgcttgaa gaaattgcaa aagaagaatc
     4081 atcaactgta aagccagtaa tgtttgaaga ttttgaaaaa catcaagtta aattattgac
     4141 taaaacaaag gtaattgaaa ttaaaaacga ttgtatcaaa gctgctaatg gcgatggtga
     4201 attaacgatt ccatgtgatt atgttatttt agctactgga gctaaaccta atccatttga
     4261 tgtcagtgaa ttaacagctc acaatattgg tattcatctt gttggtgact gcaatgaaaa
     4321 agctgctgat attaataatg ccattactca aggctattta gcagccaaca gtatttaaaa
     4381 cccttattat ctagactaaa aagaattaat cataacattt aaatgtttaa gattaattct
     4441 tttttattct tattaactat ctactattac tagccattct aaaccacgta taattttttc
     4501 ctttgattga tactgataat tgataaatat tttctggcca agtacatccg ccaaagcctc
     4561 catccccgat agcatgaata tcacatccgg tttgtttcat taaaagggca acctgccgaa
     4621 ttgtatcctc atcggcacat tcgaccgaac tatttaaaaa actcatggct aaagtacctg
     4681 gtttatagcg atgaatatat tctactaatt ctctaatcat atctactgtg attcctgctc
     4741 tagtccctgg agctggtaaa tcgatacagt cagcaccagc atcaataagt cttttaataa
     4801 catctttggt atcatatgct gctaaaggat cacctagtac cttttcttcg ataccatctt
     4861 cccacttgcc agcaaacaag aaaatatcat ctccacatat ttcttttact aatttggttg
     4921 cttcaattac atcttcaatc gatgttccag aacctggatt accacccaat acaataaaat
     4981 ccactcctaa agcaactgct tttaaaatat gttcttttgt tgctaacatt ccagccaatc
     5041 ggtaaagtgt tttttcatca gaacgctttt ctcccttctt aggacaccct aaataaacac
     5101 caattggaca attgcatttt tctttaagct ctttgatagt taagccgcac aatcctggat
     5161 tattttcaaa atcatctaaa tctatagtat taagcataac catatctgca ccaaacgaac
     5221 aaagcaattc agcatttgtc acacccatta ccgtccctgg gccagcaaaa attaaatgtt
     5281 gtcccatgat aactcgacct tccgatttat agatagattc ttttaattct aaaggaccca
     5341 ttttgtttat ctcactcttt gaagcattaa tcaatctttt tacgttgctc ataatttttc
     5401 ctcatttcta gattttgttt tcttcaagtg ttttttcttg attacttttt ttctctgctt
     5461 ctaaagcctg attgtcacaa atcttaaaaa atgggtaata aattatacat gataacaagc
     5521 acataattac aatcatgatt cctaaactaa ttcccccacc taaaataaat cccgcaattg
     5581 gacctggaat tgtcattaca cttaacatta aggcgggaat attcacaaca cctgtaacca
     5641 tcaacaaata accaataatc ataaatacaa gtggtgttaa aataaacgga ataaacataa
     5701 ttggatttaa tacgattgga aaaccaaaaa taacaggctc tacgatgtta aacatccccg
     5761 gaacaaaaca cacttttcca aacgctttaa aacgttcgct tttagcaaat attgccatat
     5821 cgcaagtaag cccaaataaa catcctggtc caccaataaa ctgacaaaaa acaaagaaaa
     5881 acatataagt tgtgactaag gttacaggtt ctccggccat ataattattt aaatttgtaa
     5941 ttgctggttc catccaaatt accatcatga cagaaagtac aactgaagta ccatgaattc
     6001 caacccacca taacagcaat aatattaaca tactaatcaa catggctgga aaactattac
     6061 ctaaacttac taaaggcgct tgaatcatag tataaataaa attttgaata tttccataag
     6121 atgttacagc aaatccagac cttaccaata aaaacaaggc tgatactaaa aatgctggaa
     6181 caacagcttc aaatggttta gaaaccattt ccggtaccga atctggtaat tttattttcc
     6241 aattatgatt aacggcaaaa gcaaaaacat atgccccaat aatcccacat aacatggcag
     6301 tgaagatacc actagcacct aagttatcaa ctgggataac attttttatc aatgttacag
     6361 ctccatttgt actatcaaca ttgttataag aaaatggtgt aaccaacaat agtgataata
     6421 acgaaacaat tgctgtttga aatggattaa cattaaactt ttcacctaaa ttataaccta
     6481 aagcaaaaat gaaatataat gctgttaaat tacaaacagc aacccctaca gctaaagcat
     6541 cagatattcc agtagacgat aaaaaatttg tataccagtc tcccaatggt aaaccctgaa
     6601 ataatgaaaa tacagctcct cctaataact ctggcatcac tttcataaat gcgccactta
     6661 tt
//
LOCUS       DAOMTA010000051         6646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_178_length_6646_cov_2.650432, whole genome shotgun sequence.
ACCESSION   DAOMTA010000051 DAOMTA010000000
VERSION     DAOMTA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_6646_cov_2.650432"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLK68_02350"
     CDS             <1..219
                     /locus_tag="PLK68_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRM89282.1"
                     /translation="PGQQMVIDTGYLAMMDATCKMEIQSVKGVKNKLLGGEGFFNTVV
                     TGPGKVVLQSMPISAVAAGLIPFLPSGK"
     gene            310..1080
                     /locus_tag="PLK68_02355"
     CDS             310..1080
                     /locus_tag="PLK68_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89283.1"
                     /translation="MKPMIFFDVDGTLMDNHDYQVTPSTKKALQKLQENGYKIGIATG
                     RAVNSLKRTGVVDIANWDGFVCNNGQTVLNKNFQIVEELVISPEIVHRCIKIAKDNNI
                     PLALKMEHRIITQEPDENVYTARKFFNSIIPPVGIYQNQKVEAMIAYGPKGYDYAPFK
                     QLEGLHILPGVSTYCDITHADATKATGIQVILKQYNLDKYICFGDSLNDVEMFNHAAI
                     SVCMGQGDAYLKNIATFVTDSIDDDGIYNACINLGLFK"
     gene            complement(1117..1917)
                     /locus_tag="PLK68_02360"
     CDS             complement(1117..1917)
                     /locus_tag="PLK68_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89284.1"
                     /translation="MTTIYYGWTQYAYNMIKSYRDDRGLDGDIYCGLFEDVSYPDETY
                     SSAHWSWIKMLNGDVYASTNASLDTITWFKSLSTSTNDYQLKTVTNPVAGEAAGDNIY
                     AYNLIATVTNSGEKITALEIDMNGKKVDASKLTTEMFKITGYNSDASGLVKGDVQSYG
                     IFGSEDEPVDIEVARVSVNEKGNIILDLATQNGVLNYTSLARNLATKIRYKLASVALP
                     MIVEDNSTTNPDDKQTAVKTGDDLNVLGAGSLMIISLMMVAVSKRKFD"
     gene            complement(1877..2698)
                     /locus_tag="PLK68_02365"
     CDS             complement(1877..2698)
                     /locus_tag="PLK68_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM89285.1"
                     /translation="MKKIKKFLSSLIAIMIVATMVTSNLSALEVNSSFVQNTNTSNTY
                     GYVNYETDQFETYSKVSGSAGNYTSIASKVYKPLSGDTLIVVFHGNGEGGVDGVSNNY
                     SQIAANRLAVTYTTDELQSAFKGAYVLAFQAPDYWYNDYTQQAKTIIDQAIAEFGIKE
                     VFVSGLSAGGLMTQRMLAKYGDFFAGALMSCAAIAKNDQYVEGLGGNYTDSTEYLDAG
                     DAYTSGDTFKKPVDFNQYLANYDEWLENIAVSNVPIFMVHCYYDNNLLWMDSICL"
     assembly_gap    2719..2740
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2795..3625)
                     /locus_tag="PLK68_02370"
     CDS             complement(2795..3625)
                     /locus_tag="PLK68_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89286.1"
                     /translation="MSHRIVVRKHEKIDYQRSTFFTLAHTHYDKDYYMENHWHNSIEI
                     TYVVKGLKVQLMENKEVIAPAGTLLLVNSGVIHDVDVKKGLEGIVLLIDRNYIDHVCP
                     QCIERGFSLEKEPLAKKKIVDYLFKLVEAYENNNKVKANIIVLEIISVLAEQLMLEGH
                     YIKEKHDDESYELVISITEYIDYHYAQKISLDDLARMTCYNKTYLSNIFKKKTGITIF
                     EYLRNVRMQHCLYELKHSDDTIVSIALNNGFANIQIFNRVFKEVYQMTPKQYREKNKK
                     "
     gene            3871..4353
                     /locus_tag="PLK68_02375"
     CDS             3871..4353
                     /locus_tag="PLK68_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HRM89287.1"
                     /translation="MNKGISVKLVVINAMIAGVYAVLTLAISPIAYSEIQFRLSEIIV
                     FLAFYNRRYIPGLTIGCIIANLFSPMGMLDIVFGTLSTIIVCIAMYLIKNRYLAAGAG
                     AIITGMIIGAELWYAFNIPYVINAIYVAVGELIVLIIGALVFKSLEKNDRLMNLLDLK
                     "
     gene            complement(4390..4854)
                     /locus_tag="PLK68_02380"
     CDS             complement(4390..4854)
                     /locus_tag="PLK68_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HRM89288.1"
                     /translation="MRYTGFVISVKDINASKKFYEELFDLEVYQDYGINITFDCGLSL
                     QQDFDWLINIEKEKIIEKANNHEICFEEENFDAFLKRLSVYPQIEYLDDVIEHSWGQR
                     VVRFYDLDKHIIEVGESMKMVINRFIDRGMTTEQISKKMDVSVADLKKLLND"
     gene            complement(4865..6298)
                     /locus_tag="PLK68_02385"
     CDS             complement(4865..6298)
                     /locus_tag="PLK68_02385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM89289.1"
                     /translation="MLKVESKYTKKYLFAILFFIIMSIICVTIFLTNINSGINEKVQE
                     ILLNEVISHRKSLEATISIQYQELEGISEYITSLNTRDMSQLINFTKAFVSKNGFDRI
                     VVAFEDGIGYLSDGSSYDISTRGYYQDTMNGGARNSEPVDSVIDGERRMALSVPIYSQ
                     HGQIIGMVMGSYDVRAISEIEFSNLYNNKGYTYLIDYSGNFLINDQKLHNYQGNFYDY
                     CQESNILSNQQLQQIKTDISNYVSGCVLTKGGKETRYLVYVPLESSEWVICYSVLVSD
                     AFQDYQFIMKYETYLLGAVAMVLTVTVLYIFIVNKKERRVLLKRADYDSLTGLYNRRK
                     LTEEIKRYLKAQSEFGLAILDIDDFKKINDTYGHPIGDIILKEIGQILRDNLGEYLVG
                     RLGGDEFVILIENVQIINIVIQKLELTCNQIAKIKIEEHPDIVVTCSCGLAVAPKDGE
                     TVAELYKAADSALYIAKSLGKNKLAKF"
     gene            complement(6326..6595)
                     /locus_tag="PLK68_02390"
     CDS             complement(6326..6595)
                     /locus_tag="PLK68_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRM89290.1"
                     /translation="MNMKRYEFDAVIKKVPNIDGAYVDFPYDVKKEFNKGRVKVIATF
                     DGIEYLGSLVKMKTPNHIIGLRKDIRKIINKQPGDIVHVTIQERE"
BASE COUNT         2195 a         1202 c          847 g         2380 t
ORIGIN      
        1 cctggtcaac aaatggttat tgatacagga tatctagcta tgatggatgc tacatgcaag
       61 atggaaattc aaagcgttaa aggagttaaa aataaactgt taggtggtga aggttttttt
      121 aatacggttg tcacaggacc aggaaaagta gttcttcaat caatgccaat cagtgccgta
      181 gcagcgggat taattccatt tttaccaagc ggaaaataat ttaattaagg aatatgttta
      241 gtcatattcc ttttttgttt acattcatag gtatgcttgc taaaatatac gcatgaaaag
      301 ggggagctta tgaaaccaat gattttcttt gatgttgatg gaacattaat ggataaccat
      361 gactaccagg ttactccatc aactaaaaaa gcattacaaa aattacaaga gaatggttat
      421 aaaattggaa ttgcaactgg tcgtgctgtt aattcattaa aacgtaccgg ggttgttgat
      481 attgctaatt gggacggctt tgtttgtaat aatggtcaaa ctgtgcttaa taaaaatttt
      541 caaatcgtcg aagaattggt tatttccccg gaaatagtcc atcgttgtat aaaaattgct
      601 aaagataata atattccact cgctttaaaa atggagcatc gaatcattac ccaagagccc
      661 gatgaaaatg tttacactgc acgcaaattt tttaactcta tcatccctcc ggtaggtatt
      721 tatcaaaacc aaaaagttga agcgatgatt gcctatggac caaagggata tgattatgcg
      781 ccatttaaac aattagaagg tttacatatc ttacccggag tctctacata ttgtgatata
      841 actcatgctg atgcaactaa agcaactggt attcaagtta tcttaaaaca atataatctt
      901 gataaatata tttgttttgg cgattcttta aatgatgttg agatgtttaa tcatgctgct
      961 atctctgttt gcatgggaca aggtgacgca tatcttaaaa acattgcaac ttttgtcact
     1021 gacagtattg atgatgatgg tatttataac gcttgtatta acttaggttt atttaaatag
     1081 ttataaaaag ttctacaaaa atgtagaact tttttcttaa tcaaattttc gtttagatac
     1141 agctaccatc atcaatgaaa taatcattaa gcttccagca cctaaaacat ttaaatcatc
     1201 gcctgtttta acagctgttt gtttatcatc aggatttgtt gttgaattat cctcaacaat
     1261 cattggtaga gctactgatg ctagtttata tctaatctta gtcgcaagat tacgagccaa
     1321 agaagtataa tttaatactc cattctgtgt tgctaaatct aatatgatat tacccttttc
     1381 atttactgat actctagcca cctcaatatc tacaggctca tcttcgcttc caaaaattcc
     1441 ataagactga acatcacctt taaccaaacc actagcatca ctattgtatc cggttatttt
     1501 aaacatctca gttgttaatt tagaagcatc aactttctta ccgttcatat caatttcaag
     1561 agccgttatt ttttcaccac tattagttac tgtagcaatt aagttataag cataaatatt
     1621 gtcacctgct gcttctccag ctacaggatt agtaactgtt tttaattgat aatcattagt
     1681 tgaagttgat aaagatttaa accatgtaat cgtatctaaa ctcgcattag ttgaagcata
     1741 aacatcacca ttcaacattt tgatccacga ccagtgagcg ctgctatatg tttcatcagg
     1801 ataactgaca tcctcaaata aaccacagta gatatctcca tcaagccccc tgtcatcacg
     1861 ataggattta atcatattat aagcatattg agtccatcca taatagattg ttgtcataat
     1921 aacaatgtac catgaaaatc ggaacattgc ttacggcaat attttctagc cattcgtcat
     1981 aattagctaa atattgattg aaatcaactg gtttcttaaa ggtatcacca cttgtataag
     2041 catcaccagc atctaaatat tcagtagaat cagtgtaatt tccacctaga ccttcaacat
     2101 attggtcatt tttagcaata gcagcacatg acattaatgc gcctgcaaag aaatcaccat
     2161 attttgctaa catccgttgt gtcattaaac cgcctgctga taatccagaa acaaatactt
     2221 ctttaattcc aaattctgct atagcttgat caataatagt ttttgcttgt tgcgtataat
     2281 cattatacca ataatctggt gcttgaaaag ctaatacata agctccctta aaagcacttt
     2341 gtagttcatc agttgtataa gttacagcta aacgattagc agctatctgt gagtagttat
     2401 tactaacccc atcaacacca ccttctccat taccatgaaa aacaacgatt agagtatctc
     2461 cagataatgg tttataaact tttgaagcaa tgcttgtata attcccagca cttcctgaaa
     2521 ccttactata agtttcaaat tgatctgttt cataatttac ataaccatat gtattacttg
     2581 tgttcgtatt ttgaacaaac gagctattta cctctaatgc acttagatta gaagtaacca
     2641 ttgttgcaac tatcattatt gcaattagcg atgataaaaa ctttttaatc tttttcataa
     2701 catcctcctc tttgacacnn nnnnnnnnnn nnnnnnnnnn gcatggcgga ttttattatt
     2761 attgttgttt atttgtttta atcttcatta atctttactt cttattcttt tcacgatact
     2821 gctttggtgt catttgataa acttctttaa atacacgatt aaatatttga atatttgcaa
     2881 atccgttatt taaagcaata cttacaatcg tatcatcaga atgttttagt tcatataaac
     2941 aatgctgcat tctgacatta cgtaaatact caaaaatagt aatacctgtc tttttcttaa
     3001 aaatatttga taaataagtc ttgttatagc atgtcattct tgctaaatca tctaaactaa
     3061 ttttttgagc ataatgataa tcaatatatt cagtaatcga aataactaat tcataacttt
     3121 catcatcatg cttttcttta atataatgcc cctctaacat taactgttca gccaaaacac
     3181 taataatttc taaaacaata atattagctt taactttatt attattttca taggcttcta
     3241 ctaatttgaa taaataatcc acaattttct ttttagctaa tggttctttt tccaagctaa
     3301 agccacgttc aatacattgt ggacaaacat ggtcaatata attacggtca atcaataaga
     3361 caatcccctc taatcccttc tttacatcaa catcatgaat tactcccgaa ttcactaata
     3421 acaaagttcc tgctggtgca ataacttctt tattctccat cagttgaacc tttaagccct
     3481 ttactacata agtgatttca atactattat gccaatgatt ttccatataa taatctttat
     3541 cataatgtgt atgggcaagt gtaaagaaag tactgcgctg ataatcaatt ttttcgtgtt
     3601 tgcgcaccac tatacgatgt gacatgttaa ccttcctttc gtaattatac atcattatta
     3661 tcgtatcaca tattttatta attaggatta atctttttga taaaaagagc aagtatttaa
     3721 ttttaaatat tgttgtattt atataaaata ttttaattct catattccta tacagaaaga
     3781 ctagtctttt tacttaaaat cgtatataat atccatgttt tcaatgaggt gacatttaag
     3841 tgaaaataat actatgaaag gatataatat atgaacaaag gtatttcagt aaaactggtt
     3901 gtaatcaatg cgatgattgc gggggtttat gctgtactca ctttagcaat ctcacccatc
     3961 gcatactcag aaatccaatt taggctatca gaaataattg tttttttagc attttataat
     4021 cgacgctata ttcctggctt aacgattggt tgcattattg ctaatctatt ttcaccaatg
     4081 ggaatgttag atattgtctt tggaacttta agtacgatta tagtatgtat tgctatgtac
     4141 ttgattaaaa atcgctattt agctgctgga gcaggagcaa ttataacagg aatgattatt
     4201 ggggctgaat tatggtatgc tttcaatatt ccatatgtaa taaacgcaat ctatgttgct
     4261 gttggagaat taattgtttt aattatcgga gcacttgtct ttaaatcatt agaaaaaaat
     4321 gaccgtttaa tgaacttatt agatttaaaa taagatcatt gacggtcttt tattacttta
     4381 atattctttt taatcattaa gcagtttttt aagatcagca actgacacat ccattttttt
     4441 tgaaatctgc tccgttgtca tcccacgatc aataaaacgg ttaattacca tcttcatcga
     4501 ctcacctact tcaataatat gtttatccaa gtcataaaaa cggactactc gttgacccca
     4561 gctatgctct ataacatcat caagatactc tatttgtggg tacacagata atctttttaa
     4621 aaatgcatca aaattttctt cttcaaaaca aatttcatga ttatttgctt tttcaataat
     4681 cttctctttc tctatattaa taagccagtc aaaatcttgc tgcaatgata acccacaatc
     4741 aaacgtaata tttattccat aatcctgata aacttctaaa tcaaatagtt cttcataaaa
     4801 tttttttgat gcattaatgt cttttactga tattacaaat cctgtatatc tcatcttctt
     4861 ctccttaaaa ttttgccaat ttattttttc ctaatgattt agcaatatat aaggcactgt
     4921 ctgcagcctt atatagttcc gctacggttt ctccatcctt tggtgcaact gctaacccac
     4981 aactacatgt gacaacaata tctggatgtt cttctatttt tattttagca atttggttac
     5041 aagtcagttc aagtttttga ataacaatat taataatttg aacattttca attaagataa
     5101 caaactcgtc accgcctaaa cgtccgacca aatattcacc aagattatct cttaaaattt
     5161 gtccaatttc tttcaaaata atatcaccaa taggatgacc atatgtatca ttgattttct
     5221 taaaatcatc aatatctaaa atcgctaatc caaattcact ttgagctttt aaatacctct
     5281 taatttcctc tgttaatttc cgacgattat ataaacctgt caaactatca taatcagctc
     5341 tttttaacaa aacacgacgc tcttttttat ttacaataaa aatataaaga accgtaactg
     5401 ttagaaccat tgcaacagct cccagcaaat atgtctcata tttcataata aactgataat
     5461 cttgaaatgc atcagagact aatacagaat aacaaattac ccactcactt gactctaaag
     5521 gaacatatac taaatagcgt gtttctttac ctcctttagt caaaacacat cctgaaacat
     5581 aattactaat gtctgttttt atctgctgta gctgctgatt acttaaaata ttactttcct
     5641 gacaatagtc ataaaaattt ccttgataat tatgaagctt ctgatcatta atcaaaaaat
     5701 taccagaata atcaattaaa taagtataac ccttgttatt atataaatta gaaaactcaa
     5761 tttcactaat tgcccgtaca tcataagatc ccattaccat accgattatt tgaccatgtt
     5821 gactataaat tggaacagat aaagccatcc tacgctcacc atcaataact gaatctactg
     5881 gttcactatt tctagctcct ccattcatcg tatcttgata atatcctcta gtacttatat
     5941 cataactact accatcactt agatatccaa ttccatcttc aaacgccaca acaattcgat
     6001 caaatccatt tttagaaaca aatgctttag taaaatttat caattgtgac atatcacgtg
     6061 tgtttaagct agtgatatac tcagatatcc cttctaattc ttgatattgt atagaaatcg
     6121 tcgcttccaa acttttgcga tgagaaatca cttcgttaag taaaatttct tgaacttttt
     6181 cattaatccc actattgata ttagttaaaa aaatagttac acatataatt gacattataa
     6241 taaaaaacaa gatggcgaat aggtacttct ttgtgtattt agactctact tttagcattt
     6301 ttttcacccc ttgttttatt tactattatt cacgttcttg aatcgttacg tgaacaatat
     6361 ctcctggttg tttattaatt atttttcgaa tatcttttct caacccaata atatgattag
     6421 gggttttcat ttttactaaa cttcctaaat attcgattcc atcaaatgta gcaatgactt
     6481 ttacacgtcc tttattaaat tcctttttta catcgtatgg aaaatcaaca taagcaccat
     6541 ctatattcgg tactttttta attacagcat caaattcata tcttttcata ttcattttat
     6601 cacttccata tgtaattgta acctatatca agaaaataat ctaatt
//
LOCUS       DAOMTA010000052         6645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_180_length_6645_cov_2.306677, whole genome shotgun sequence.
ACCESSION   DAOMTA010000052 DAOMTA010000000
VERSION     DAOMTA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_6645_cov_2.306677"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLK68_02395"
     CDS             <1..231
                     /locus_tag="PLK68_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM89291.1"
                     /translation="ALMLSGMQAGVGAVIASVAYDMAVGVVKEKEILPIIIMVCAFIA
                     TYLFNVNVIYVILSCGFIGIIQNMVSRWRERQ"
     gene            228..788
                     /locus_tag="PLK68_02400"
     CDS             228..788
                     /locus_tag="PLK68_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM89292.1"
                     /translation="MKLLTLFFSFLQIGAFSFGGGYAALPLIQHQVVDLYHWLTMNEL
                     TDLITISQMTPGPIAINAATFVGLKIDSFYGAIVATLGCILPSCIIVSLIAYIYLKYQ
                     QMSIIQNILKYIRPAVVSLIAVSGLLIIVSCFFGDTVNFANLKFGSVAIFIVALVLLR
                     KQKMNPITVMVIAGIIQIGLYYIGIS"
     gene            873..1778
                     /locus_tag="PLK68_02405"
     CDS             873..1778
                     /locus_tag="PLK68_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRM89293.1"
                     /translation="MYKRILVVVTVLSCLLTGCSSDNNSNQEYPSDYPTSKSYTVDEI
                     LYPEASGALVLDSDVAKVDYSNTNQGYVMAFLKPGVSKRIKIQISKDEQKLNYDLTDE
                     QGVSYPLQLGNGKYLIKILENIEGTQYAIKKSVEVDVILDNELLPFLYPNILVNYKPG
                     DAITTLAIDEVKDEDNDLKRIKKVYEFVANYINYDDDKVALAKQQYLIPNLDEIINNK
                     KGICFDYASMMTAMLRINHIPARLICGGTDKDEYHSWLEVYVKGQGWVNPDIFMDKDT
                     WTIMDPTFASTKYDYEGKYVETARY"
     gene            1790..2818
                     /locus_tag="PLK68_02410"
     CDS             1790..2818
                     /locus_tag="PLK68_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM89294.1"
                     /translation="MKLTMSEKYMFCNQMAMILESGFSLNQGVTMVYEEMDDKNIKGV
                     LQEVAKYLDEQVSFSEAIDLTKAFDDYMVNLVKVGETSGNLDDVMQSLSEYYARIDDI
                     TNKLKQALTYPIILIIMMVVVVGIIVFKVLPIFKDVLNGLGSDLSSYANSFMEFGQIF
                     SLICFAVLLVLVIVIIAGYLYQRVTHVNVLSNVVQKSFLTRKLSRALNKAQITYALSL
                     FISSGYDLQEAMKFVPKLVDDKQLRANLEKCNEDLINGDSFVEVIKKYQIYQGMQLNM
                     IQVGFKTGQVDIIMKQLSNSFQEEVSRAIDQFLNIIEPTIVTLLSLVVGIVLMSVMLP
                     LISIMSSL"
     gene            2829..3128
                     /locus_tag="PLK68_02415"
     CDS             2829..3128
                     /locus_tag="PLK68_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89295.1"
                     /translation="MKNMLKIILLIVIIMVSIYAFNSTSTYSSNEDLKRVSDTINQLS
                     LKCYSIEGKYPKDIEYLKENYGLLLNDEDYQVIYYYEGDNLQPRIEVFKKEKNYE"
     gene            3121..3570
                     /locus_tag="PLK68_02420"
     CDS             3121..3570
                     /locus_tag="PLK68_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HRM89296.1"
                     /translation="MNKHRNIHVLFSLSLFLLFVIGSFFIVTYEIKGYQVINDTCQQE
                     DDLIVPLAYLNTKLKANDSDDSTKIVEIDNTQCLEIKTAKTVTYIYCQDGYLKELYTS
                     NDYHAGLQEGSKLFALDDFKIEQKDRLFKFTVTRDQVSKSISIYLHG"
     gene            3580..3948
                     /locus_tag="PLK68_02425"
     CDS             3580..3948
                     /locus_tag="PLK68_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89297.1"
                     /translation="MKSKVQSFSFLMELIIVILFFAASTTVCASFIVQAKNKQVQGTN
                     LQNALIEAQSMIETMQAYPQADLEQLLEVEKIDENHYQKDNIFIEIDRDMITQGKIMI
                     KNKNEVISELPFVLGGNHDE"
     gene            3941..4321
                     /locus_tag="PLK68_02430"
     CDS             3941..4321
                     /locus_tag="PLK68_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89298.1"
                     /translation="MNKKHTSMRLGIGAPSIFMIFVILVMCVLAILSYLRANSYYESS
                     VRQANITSQYYESESRLLTKYYQLDSQNLEYSLENLQIEYQKEDDLYMLEDKINDSQV
                     LQLSFVQENDSLKIISLKTINLEE"
     gene            4323..5378
                     /locus_tag="PLK68_02435"
     CDS             4323..5378
                     /locus_tag="PLK68_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRM89299.1"
                     /translation="MEIKKLLEEVVTKGASDIFIVAGSPCAIKIDGRISKYSDERLTP
                     HDTERMIRDIYEIANNAGIEEFMRSGDDDFSFSIPNVGRFRVNAYRQRNSCAAVLRVV
                     SFQLPDPEVMHIPKVITDLANQKKGMVLVTGPAGSGKSTTLACIIDQINRNRNTHIIT
                     IEDPIEYLHSHDKSIVSQREVYHDTHDYVSALRAALREAPEVILVGEMRDLETIDIAV
                     TAAETGHLVFSSLHTVGAANTIDRMIDVFPPSQQQQIRVQLAMVLNAVISQQLVPGID
                     GKMIPVFEIMICNQAIRTLIRDGKTHQIDNAISTNRQIGMVTMDDAIVDLYKQNKITK
                     ETAVMYASNPNLIERKF"
     gene            5523..>6645
                     /locus_tag="PLK68_02440"
     CDS             5523..>6645
                     /locus_tag="PLK68_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRM89300.1"
                     /translation="MDKKLVLKIKTLGRLHITDGENEFPQEKKRSVQVELLIVYLILN
                     RNANITNPQLIDFLWSDGNADKPEGALRNLIYRARKEMKQFFKEVDCIKSKGRSYYWN
                     PEVECRVDYEEMLKLCRRVEQEDDPYRKHERCLEMINKYHGEVLPEFNYNDWILEINN
                     LLERNCLEAILKTLTILANNNLYEQVLQICNHQNSQKIMDTRLYEMKLYAYYKTGKTD
                     LALSFYRQIVDYYYSKYGIEVSQRLKEIYEMILDTSPATQIDVEELEKSLTVDDNNDN
                     TFYCDFDVFKNIYQINVRSARRSMKARILTLLTIVDTSGCLDEKAIRQEADILREVIS
                     NSLRKNDVYSKFNMTQYSLILASPDLDGAKIAVDRITNRY"
BASE COUNT         2328 a          800 c         1289 g         2228 t
ORIGIN      
        1 gccttaatgc tttctggaat gcaggctgga gttggggcag taattgcttc agttgcttat
       61 gatatggcag taggggttgt taaagaaaaa gaaatcttac caattataat tatggtatgt
      121 gcttttattg caacatatct ttttaatgta aatgttattt acgtaatctt aagttgtgga
      181 tttattggaa ttatacaaaa tatggtaagc agatggaggg aaagacagtg aaacttttaa
      241 ctttgttttt cagttttctt caaattggtg catttagttt tggaggagga tatgctgctt
      301 taccgctgat tcagcatcaa gtagttgatt tatatcattg gttgacgatg aatgagttaa
      361 cagatttaat aacgatatcc cagatgacac ctggaccgat tgcaattaat gcggcaacat
      421 ttgttggatt aaagatagat agtttttatg gggctatagt agcgacattg ggatgcattt
      481 taccttcatg tattattgta tcgctgattg cttatattta tttaaaatat cagcaaatgt
      541 caattattca aaatatttta aaatatattc gtccggctgt agtgtcgctg attgcagttt
      601 caggactatt gattattgtt agctgttttt ttggagatac agtaaacttt gcaaatttga
      661 aatttggttc tgtagccata tttatagttg ctctggtttt attaaggaaa cagaaaatga
      721 acccgattac agtgatggta atagctggaa tcatacagat tggtttatat tatataggaa
      781 ttagttaagg tagagagagt ttttcttttt gcctttttct tttcaatccc agatattttg
      841 gtgtataatt aaaattaagg aggataatgg taatgtacaa gcgaatacta gtggtagtca
      901 ctgtattatc atgtcttctt actggatgtt caagtgataa taacagtaat caagaatacc
      961 caagtgatta tccaacatca aaaagttata cagttgatga gattctttat cctgaagcat
     1021 caggggcact ggttttagat agtgatgtag cgaaggttga ttattctaat actaatcaag
     1081 gatatgtaat ggccttttta aaaccaggtg ttagtaaacg aataaaaatt caaattagta
     1141 aagatgaaca aaaactaaat tatgatttaa cggatgaaca aggagtgagt tatccactcc
     1201 agttgggaaa tggaaaatat ttgattaaaa ttttagaaaa tattgagggt acccaatatg
     1261 caataaaaaa aagtgtggaa gttgatgtta ttttagataa cgaactgctc ccctttttat
     1321 atcctaatat cttggttaat tataaaccgg gtgatgcgat tacgacgtta gctattgatg
     1381 aagtgaagga tgaagataat gatttaaaac gaataaaaaa agtctatgaa tttgtggcta
     1441 attacataaa ctatgacgat gataaagtag ctttagcaaa acagcagtat ttgattccaa
     1501 atttagatga aataattaac aataaaaagg gcatatgttt tgattatgct tcaatgatga
     1561 cggcaatgtt aagaatcaat catattccag cccgactgat ttgtgggggg actgataaag
     1621 atgaatatca ttcttggtta gaagtttatg ttaaagggca gggttgggtc aatccggata
     1681 tttttatgga taaagataca tggacgatta tggatccgac tttcgcaagc actaagtatg
     1741 attatgaggg aaaatacgtt gaaacagcac gttattaagg agaaaaacga tgaaattgac
     1801 gatgagtgaa aaatatatgt tttgtaacca gatggcaatg atcttagaat cgggatttag
     1861 tcttaatcaa ggggttacga tggtatatga agaaatggat gataaaaaca ttaaaggggt
     1921 tcttcaagaa gttgctaaat atttagatga acaagtaagt tttagtgaag caatcgatct
     1981 aactaaagct tttgatgact atatggttaa tttagtcaaa gtgggtgaaa cgagcggtaa
     2041 tttagatgat gttatgcaat cattaagtga atattatgct cgaattgatg atataacaaa
     2101 taaactgaaa caggctttaa cttatccaat cattttaatt attatgatgg tggtggttgt
     2161 aggaatcatt gtatttaaag tattaccaat ttttaaagat gttttaaatg gactaggcag
     2221 tgatttatca tcgtatgcta atagttttat ggaatttggt caaatttttt cattgatatg
     2281 ttttgcggtc ttgttagtgc tggtgatagt aattattgct ggatatttat atcagcgcgt
     2341 tacccatgta aatgtcttgt caaacgtagt gcagaaatct tttttaacaa ggaaattgtc
     2401 aagagcatta aataaagctc aaattactta tgctttgtca ttgtttattt ctagtggtta
     2461 tgatttacaa gaggcaatga agtttgtacc aaaattagtt gatgataaac agcttagggc
     2521 taatttagaa aaatgcaatg aagatctcat aaatggtgat agtttcgttg aagttatcaa
     2581 aaaatatcaa atttatcaag gaatgcagtt aaatatgatt caggtcggtt ttaaaactgg
     2641 acaggttgat ataatcatga aacaattatc aaatagtttt caagaagaag ttagtcgtgc
     2701 gatagaccaa tttttaaata tcatagaacc aacgattgta actttgttat ctttagtagt
     2761 gggaatagtc ttgatgtcgg taatgttgcc attaattagt atcatgtcct ctttatagga
     2821 ggttatttat gaaaaatatg ttaaagataa tcttactgat agttataatc atggtaagca
     2881 tttatgcttt caattctact tcaacatatt catcaaatga ggatctaaaa cgggttagtg
     2941 atacaattaa tcaacttagt ttaaaatgtt atagtattga aggcaaatat cctaaagata
     3001 ttgagtattt gaaggaaaat tatgggttgc ttttaaatga tgaagattat caggttattt
     3061 attattatga aggagataat ttacaaccac gaatcgaggt atttaaaaag gagaagaact
     3121 atgaataaac atcgtaatat acatgtatta ttttcgctgt cattgttctt gctttttgta
     3181 attgggtctt tctttattgt tacttatgaa ataaaaggat atcaagttat taatgatacg
     3241 tgtcaacaag aagatgattt aattgtgcct ttggcttatt taaacacgaa gttaaaagca
     3301 aatgatagtg acgattcgac taaaattgtt gaaatagata atacgcaatg tttagaaatt
     3361 aaaactgcta agacggttac ttatatttat tgtcaagatg gttatctcaa ggaattatat
     3421 acaagtaatg actatcatgc ggggttacaa gagggtagta agttatttgc attagatgat
     3481 tttaaaatag aacaaaaaga ccgtttattt aaatttacag ttacacgtga tcaagttagc
     3541 aaaagtattt caatttattt acatggatag gaggaaagta tgaaatcaaa agtacagtca
     3601 ttttcgtttt taatggaatt aatcatagtt atcctctttt ttgctgcttc aacaacagta
     3661 tgtgcctcat ttatagtgca agctaaaaat aaacaagtac aagggactaa tttgcaaaat
     3721 gcattaattg aggcgcaaag tatgattgaa acaatgcaag cttatccgca ggctgattta
     3781 gaacaattat tggaagtaga aaaaatcgat gagaatcatt atcaaaaaga taatattttt
     3841 attgaaatag atagagatat gattactcaa ggaaaaataa tgataaagaa taaaaacgaa
     3901 gttatcagtg aattgccttt tgtattagga ggtaatcacg atgaataaga aacatacttc
     3961 aatgcgtttg ggaattgggg cgccatcaat ttttatgatt tttgtgattt tagttatgtg
     4021 tgtactggca atattatcat atttgcgagc taattcttat tatgagtcaa gcgttcgcca
     4081 agctaatatt actagtcaat attacgaaag tgagtcacgg ctgttaacca aatattatca
     4141 attagatagt caaaatttag aatatagttt agaaaatctt caaatcgagt atcaaaaaga
     4201 agatgattta tatatgcttg aagataaaat aaatgattct caagttttac agctgtcatt
     4261 tgtgcaagaa aatgatagtc tcaaaataat tagtttaaaa acaattaatc tggaggaata
     4321 aaatggaaat aaaaaaatta ttagaagaag tagtaactaa aggggcttct gatatcttta
     4381 ttgttgcagg ttcgccttgt gctattaaga tagatgggag aatttcaaaa tatagcgatg
     4441 aacgtttgac accacatgat actgagcgga tgattcgtga tatttatgaa attgctaata
     4501 acgctggaat tgaagaattt atgagaagtg gcgatgatga tttttctttt tctattccta
     4561 atgtagggcg gtttcgggtc aatgcttatc gtcaaagaaa ctcttgtgcc gcagtattac
     4621 gtgttgttag ttttcaactt cctgatcctg aagtaatgca tattccaaaa gtaattactg
     4681 acctagctaa tcaaaagaaa ggaatggtct tagtaaccgg cccggcagga agcggtaaat
     4741 ctacgacatt agcctgtatt attgatcaga tcaatcgtaa tcgaaataca cacattatta
     4801 caattgaaga tcctattgaa tatttgcata gtcatgacaa aagtatcgtt tcacaacgag
     4861 aagtttatca cgatacccat gactatgttt ctgcgttaag agcagcttta cgggaggcgc
     4921 cagaagttat tttagttggt gaaatgcgtg acttagaaac tattgatatt gcagttacgg
     4981 cagctgagac ggggcatttg gttttttcaa gtttgcatac agttggtgct gctaatacga
     5041 ttgatcgaat gattgatgtg tttcctcctt cacagcagca gcaaattcga gttcagctgg
     5101 caatggtact taatgcagtc atctcccaac aattagtacc aggtattgat ggtaaaatga
     5161 tacccgtatt tgaaatcatg atttgtaatc aagcaattag aacattgatt cgtgatggta
     5221 aaacacacca aattgataat gctatatcta ctaatcgcca aattggtatg gttactatgg
     5281 atgatgcgat cgttgattta tataaacaaa ataagattac aaaagagaca gcagtcatgt
     5341 atgcatcaaa tccgaatttg attgaaagaa aattttaatt atatattatt ttggttacaa
     5401 aacggttata ttttatttaa agtcttgaaa aaagacgatt tatttttttg aataagaaaa
     5461 tatcataatt ggaatatagt ataattatga tgttgaaaca tgtaaacaag ggagtgattt
     5521 ttatggataa aaaattagta ttaaaaatca agaccttagg tagactgcat atcactgatg
     5581 gggaaaatga atttcctcaa gaaaagaaac gaagtgttca agttgagttg ttgattgtat
     5641 atttaattct taatcgaaat gctaatataa ctaatccaca actgattgat tttctttggt
     5701 cggatggaaa tgctgataaa ccggaaggag cattaagaaa tctgatttat cgagctcgaa
     5761 aggaaatgaa acagtttttt aaagaagttg attgtattaa atctaaggga cgaagttatt
     5821 attggaatcc tgaagttgaa tgtcgagttg attatgagga gatgttgaaa ctttgtcgtc
     5881 gtgttgaaca ggaagatgat ccttaccgca agcatgaacg ctgtttagaa atgatcaaca
     5941 aatatcatgg agaggtttta ccagagttta attataatga ctggatctta gaaataaata
     6001 atttgttaga acgtaattgt ttagaggcaa ttttaaaaac tttaacgatt ttagctaaca
     6061 ataacttata tgaacaggtt ttacaaattt gtaatcacca aaacagtcaa aaaataatgg
     6121 atacacgatt atatgaaatg aaactttatg cttattataa gacaggtaaa acagatcttg
     6181 cattatcatt ttatcgtcaa atcgtagatt attactatag taaatatgga attgaagtgt
     6241 cacagcgact gaaagaaatt tatgaaatga ttctagatac ttctcctgcg acacaaattg
     6301 atgttgaaga attggaaaag agtctaacag ttgatgataa caatgataat acattttatt
     6361 gtgattttga cgtttttaaa aatatttatc aaatcaatgt tcgttcagca agacgttcga
     6421 tgaaagcacg aattttaact ttacttacga tcgttgatac aagtggttgt ttagacgaaa
     6481 aggcgattcg ccaagaagca gatattttaa gagaagtgat ttcaaatagt ttgagaaaaa
     6541 atgatgttta ttcaaagttt aatatgacac aatattcatt gattcttgcc tcaccggatt
     6601 tagatggcgc aaaaatagcg gttgatcgca ttactaatcg ttata
//
LOCUS       DAOMTA010000053         6612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_186_length_6612_cov_2.659143, whole genome shotgun sequence.
ACCESSION   DAOMTA010000053 DAOMTA010000000
VERSION     DAOMTA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186_length_6612_cov_2.659143"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PLK68_02445"
     CDS             <1..1374
                     /locus_tag="PLK68_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRM89301.1"
                     /translation="CNGQKKCGKCKVKATNRIIEVNHDDLKLLTKKELDQGYRLACSH
                     LYHQGDRFILPQADGVIEDSIYLNDTVVITEPVEKVGIIIDIGTTTVAMKWLNLKSGM
                     IIESQSFFNPQGKFGSDVIARIDFDNRDNDHKLGELIIKEIFSRINVSTKIKEILVCG
                     NATMINLFLKEKVKTIGVSPFDVPILTMTEYPLNYFIKSSVKIEVMTMNHISAYVGSD
                     IVMGIYATNMDKNKENVLLMDLGTNGEMVIGNKHRLLATSCPAGPAFEGVNIECGGPS
                     IAGAVCATKVENNKLVYKTIDNQDANSICGSGLISLIANLLRLGIIDDTGNFLNKQKK
                     YYLNDEVYLSIKDIKAFMLAKAAIQAGKEVLLKELNDEVTTIYIAGGFGNYLEKADLV
                     TLNIISSEEVNKVQYIKNSAISGLYKLILTRDFQRVQHISNQTKVIYLEKDPDFNDYW
                     IDAMVFS"
     gene            1371..2012
                     /locus_tag="PLK68_02450"
     CDS             1371..2012
                     /locus_tag="PLK68_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HRM89302.1"
                     /translation="MIKVIACKIYEYYISQLDLSLDNYEFVYLDIQQHNQPHTLAKNI
                     QKEIDKSRGDEKIIVLYGLCGNALVEIQARNIPVYLVRVHDCLTVLLGSKQRFKQLFS
                     NRLSSSWSCYSLKVNGCNVYEDEQYLKWCQRYDQETADYLKSQLQTQDNVYLIYNYCD
                     DVPFLKNQEQIKIDLKFLKQILLQTSNELVILEKNQKVVISSDIDQVFEIMEE"
     gene            2015..2647
                     /locus_tag="PLK68_02455"
     CDS             2015..2647
                     /locus_tag="PLK68_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HRM89303.1"
                     /translation="MIEEISELIQKGQAKKVVKLVNEALEAGFPATDILNQGLLKGMD
                     AIGVKFKNEEVFVPEVLVAARAMNKGIEILKPYLQDTEHQSKGTVILGTVKGDLHDIG
                     KNLVKIMLEGKGLEVVDLGVDVDAQKFVEEAKIHQAQIICCSALLTTTMPEMKKVVDL
                     VAQEHLNVKVMIGGAPVNQDYCNEIGADYYTDDATSASEVAYAIVGGTLC"
     gene            2641..3738
                     /locus_tag="PLK68_02460"
     CDS             2641..3738
                     /locus_tag="PLK68_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89304.1"
                     /translation="MLTKAKKEQLVEDFEKWWEHKLERPIIQVTLFDEDYQPQKHRYN
                     RGELLEMLYDLDKPVNEVVQAYQETFFSNIYLGDAFPIFYMRSTGVLGAYLGQTYHID
                     VKQGTIWFQEMKGCELEDIHPRLEETYPLYQRSLELIKAFNQFYGDDIAMGIANLGGM
                     MDIVESMRGANNSLIDLYDDPDEVQRLNDDIYKVFEQAYEEMIASIDLNNTLGYTGWI
                     SLLSQKPYFISQCDFCCMIGPEQFDEFVFETLKKEASLIERSFYHLDGPGAVRHLDKI
                     IECGFKGIQWINGAGAKPLNDPCWNEIYQKVHDAGLLLQVNISGKEELEYIDYIVDYL
                     GSPKGIAFICTGSSKDKEVFEAYLEKYHIPN"
     gene            3828..4091
                     /locus_tag="PLK68_02465"
     CDS             3828..4091
                     /locus_tag="PLK68_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89305.1"
                     /translation="MQLPEKVELAFLPTPIYKLEKLSKQFQKNIYIKRDDLTGIETLG
                     NKIRKLEYSLREAFEQGCDLALLVGVCSQIMLVQQPMPQLSYQ"
     gene            4037..4804
                     /locus_tag="PLK68_02470"
     CDS             4037..4804
                     /locus_tag="PLK68_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM89306.1"
                     /translation="MQSNHARATAYAAAKLSMKSCLLLRGNGSSEPVEGNYFLDRLVG
                     ADIVIKEPEIFNRDKDKIMLKLKTAYEAKGYKPYIIPMGASNGIGTLGYVEAFTEILK
                     QEEAMKVEFDTIIDAVGSGGTYAGLYIGNELNRTKKQIIGFNVCDDKEYFIKEITKII
                     KEAQVYFDQEIKTERIKIIDGYVGQGYALSRSEELDAIASLAKLEAVVLDPVYTGKAY
                     YGLINELEKGTFVDSENILFMHTGGIFGLFPKQSQFK"
     gene            complement(4877..5467)
                     /locus_tag="PLK68_02475"
     CDS             complement(4877..5467)
                     /locus_tag="PLK68_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM89307.1"
                     /translation="MNNKTLKIATIGLMAALAYVSFTFLQIKIPTPGGTTSFHLGNTF
                     CVLAALLLGGLPGGIAGAIGMGIGDLLDPVYVTYAPKTIILKLMIGIVTGLVAHKIFK
                     IEKHDNDSHLIIYVVISCMAGMFFNVVGEPIFGYFYNSMILGAPEKAAATLASWNAIT
                     TSVNAVITVALASTIYLVIRPRLAGSGILVKLAPHK"
     gene            5634..>6612
                     /locus_tag="PLK68_02480"
     CDS             5634..>6612
                     /locus_tag="PLK68_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRM89308.1"
                     /translation="MSGLKFKNIEELMDKLNNEYKVLLDVIDNVILVIDNEMKILFVN
                     RKGRKLIGENVLMQPCKALKMDNCSTEKCCIMRYLHGLQPLDNLHKDGSVEKVTVSRF
                     YDNQNNPQGFIIVATDITELSNMKKELLIGEEIYKLALKQANTTLWQYDVLNHTIEQL
                     FCPDEVALGILDINKTYYNIPESLVEAKIISQEDGLRVRKLCQEIEKGRPETSIELKM
                     KRGDGEERWISLKCSTIFDEQGRAVKSIGIGKDITDFVELKSKYEIEREYREALGKDA
                     LSYIEVNLTMNEVIDRKIAKNNFIDFYDGKNYEKSILKLSENSIPSEYGK"
BASE COUNT         2442 a          809 c         1282 g         2079 t
ORIGIN      
        1 tgtaatggtc aaaaaaaatg tggaaaatgt aaggtaaaag ctaccaatcg aatcatcgag
       61 gttaatcatg atgatctaaa actgcttact aaaaaagagc tggatcaagg atatcgttta
      121 gcctgcagtc atttatatca tcagggtgac cgatttattt tgccacaagc agacggagtt
      181 attgaggata gtatatattt aaatgatacc gtagtaatta ccgaacctgt tgagaaagtt
      241 ggaattatca ttgatattgg aacaacaaca gtagcaatga aatggctcaa tttaaaaagc
      301 ggaatgatta ttgaaagtca gtctttcttt aatcctcaag gcaaatttgg aagtgatgtg
      361 attgctcgaa ttgattttga taatcgtgat aatgatcata aattaggtga actaataatt
      421 aaagaaatat ttagtcgaat caatgtgtct acaaaaatca aagaaatatt agtttgtggt
      481 aatgcaacaa tgattaattt gtttttaaaa gaaaaggtaa aaacaattgg agtatcacca
      541 tttgatgtac caatcttaac aatgactgaa tatcctttaa attattttat aaagagttca
      601 gtaaagattg aagttatgac tatgaatcat atttctgctt atgtggggag tgatattgtc
      661 atgggaattt atgctactaa tatggataaa aacaaggaaa atgtattact gatggatttg
      721 ggaactaatg gtgagatggt gataggaaat aagcatcggc tattagcgac atcttgtcct
      781 gctgggcctg cctttgaagg ggtaaatatt gaatgtggtg gtccaagtat tgctggcgct
      841 gtttgtgcga ctaaagtcga aaataataag cttgtctata aaacaattga taatcaagat
      901 gctaatagta tctgtggtag tggtttaatt tctttaatcg ctaatttgct acgtttagga
      961 attattgatg atacaggtaa ttttttaaat aagcaaaaaa aatactattt aaatgatgaa
     1021 gtatatttat caattaaaga tataaaagct tttatgttag caaaagcagc aattcaagcg
     1081 ggaaaagaag tattattgaa ggaattgaat gatgaagtaa caactattta tattgcgggt
     1141 ggcttcggaa attatttgga gaaagcggat cttgttacct tgaatattat tagttcagaa
     1201 gaagttaaca aagttcaata tattaaaaat agtgctatca gtggtcttta taaactaata
     1261 ttgacacgag attttcagcg agttcaacat attagtaatc aaacaaaagt tatctattta
     1321 gaaaaagatc ctgattttaa tgattattgg atagatgcga tggtgttttc atgataaaag
     1381 ttattgcttg caaaatatac gaatattata tttcacaact agacttatca cttgataatt
     1441 atgaatttgt atatctagat atacaacaac ataatcaacc acatacttta gcaaaaaata
     1501 tccaaaaaga aattgataag agcagggggg atgaaaaaat aattgtttta tacggacttt
     1561 gcggtaatgc tttagttgaa atacaagcac gcaatattcc tgtatatcta gttagagtgc
     1621 atgattgttt aactgtttta ttggggagca aacagcgatt taaacaatta ttttcaaatc
     1681 gcttgtcttc ttcatggagc tgttatagtt taaaagttaa tggctgtaat gtttatgaag
     1741 atgaacaata tttaaagtgg tgtcagcgat acgatcaaga aacagcagac tatttgaaat
     1801 cccaattaca aacgcaggat aatgtatacc taatttataa ttattgtgac gatgttcctt
     1861 ttcttaaaaa tcaggaacaa attaaaatag atttaaaatt tttaaaacaa atattattac
     1921 aaactagtaa tgaattagta attttagaaa aaaatcaaaa agttgtaatt agtagtgata
     1981 tcgatcaagt atttgagata atggaggagt agaaatgatt gaagaaatta gtgaattaat
     2041 tcaaaaagga caagctaaaa aggttgtcaa attggtaaat gaagctttag aagctggctt
     2101 tccagcaacg gatattctta atcaggggtt gttgaaaggt atggatgcca ttggtgtaaa
     2161 atttaaaaat gaagaagttt ttgttcccga agtattagtt gcagctcggg cgatgaataa
     2221 gggaatcgag attttaaagc catatttaca agatacggaa catcaaagta agggaaccgt
     2281 tattctcggt actgtaaaag gtgatcttca cgatattgga aaaaatttag ttaagatcat
     2341 gttggaagga aaaggtttag aagtagttga tttaggtgtc gatgtagatg cacaaaaatt
     2401 tgttgaagaa gcaaaaatac atcaagcaca aattatctgc tgtagtgctc ttctaacaac
     2461 gacaatgcca gaaatgaaaa aagtagttga tttagttgct caagaacatt taaatgttaa
     2521 agttatgatt ggtggagcac ctgttaatca agattattgt aatgaaatag gcgctgatta
     2581 ttatacagat gatgccacta gtgcaagtga agttgcttat gcaatcgtgg gaggtacgtt
     2641 atgttaacta aagcaaaaaa agaacagtta gttgaagatt ttgaaaaatg gtgggagcat
     2701 aaattagaac ggccaattat tcaagtaact ttatttgatg aagattatca acctcaaaaa
     2761 caccgatata atcgagggga attattagaa atgctatatg atttagataa gccggttaat
     2821 gaggtagttc aagcatatca agaaacattt ttttcgaata tttatttggg tgatgctttt
     2881 cctatctttt atatgcgctc aaccggagtt ttaggagcct atttaggtca aacttatcat
     2941 attgatgtaa aacaagggac aatttggttt caagaaatga aaggatgtga gttagaagat
     3001 attcatcctc gcctagagga aacatatccc ttatatcaaa gatcattaga actaatcaaa
     3061 gcatttaatc agttttatgg agatgatatt gcaatgggaa ttgctaatct tggaggaatg
     3121 atggatatcg ttgaatcaat gagaggggca aataactcac taattgattt atatgatgat
     3181 cctgatgaag ttcaacgttt aaatgatgac atttacaaag tatttgaaca agcctatgaa
     3241 gagatgattg ctagtattga tttaaataat actttgggtt ataccggatg gatcagtcta
     3301 ttatcacaaa aaccatattt tatttctcaa tgcgattttt gttgtatgat tggtccagag
     3361 caatttgatg agtttgtttt tgaaacctta aagaaagagg catccttgat tgagcgttca
     3421 ttctatcatt tagatggtcc tggtgcagta cgtcatcttg ataagatcat tgaatgtggt
     3481 tttaaaggaa ttcaatggat caatggtgct ggagcaaaac cattaaatga tccttgctgg
     3541 aatgaaattt atcaaaaagt acatgatgcg ggcttactac tacaagttaa tataagtggc
     3601 aaagaagaat tagaatatat tgattatatt gttgattatc ttggaagtcc taaaggtatt
     3661 gcttttattt gtactggcag cagtaaagat aaagaagtct ttgaagctta tttagaaaaa
     3721 tatcatattc ctaattaagt accataatcg tggtactttt tttaaaagct tcacgtgaaa
     3781 ctattcaatt atgatataat aaaaaataag gataaggggg ataaattatg cagttaccag
     3841 aaaaagtaga actggctttt ttaccaacac caatatataa actagagaag ttatctaaac
     3901 aattccaaaa aaatatttat ataaaaagag atgatttaac tggaatagaa acattgggta
     3961 ataaaattag aaagttagaa tattcattga gagaagcatt tgaacaaggt tgtgatttag
     4021 cattacttgt gggggtatgc agtcaaatca tgctcgtgca acagcctatg ccgcagctaa
     4081 gttatcaatg aaaagctgct tattacttcg tggcaatgga agtagtgagc ctgtcgaagg
     4141 aaactatttt ttagaccgct tagttggtgc agatattgtt atcaaagaac ctgaaatttt
     4201 taatcgtgat aaggataaga tcatgctgaa attaaaaact gcctatgaag ctaaaggata
     4261 taaaccatat attattccaa tgggagcaag taatggaatt ggtactttag gttatgttga
     4321 agcttttaca gaaatcttaa aacaagaaga agctatgaaa gtagaatttg atacaattat
     4381 tgatgctgtt ggcagtgggg ggacttatgc aggtttatat attggaaatg agcttaatcg
     4441 tactaaaaaa caaattattg gatttaatgt ttgcgatgat aaggaatact ttattaagga
     4501 gataacaaaa attattaaag aggcgcaagt atattttgat caggaaataa aaacagagcg
     4561 gatcaagata attgatggtt atgttggtca gggatatgct ttaagtcgaa gtgaagaact
     4621 tgatgctatt gcatctttag ctaaattaga agcagtagta ttagatcctg tatatactgg
     4681 taaggcatac tatggtttga tcaatgaatt agaaaaagga acgtttgttg atagtgaaaa
     4741 tattttattt atgcatactg gtggaatctt tgggctattc ccgaaacaaa gtcaatttaa
     4801 ataaaaaaag gtagtattat gaaatggaca accaaataac agtgtccatt tcataatact
     4861 accttttaat aaaagcttat ttatgaggtg ctaattttac caggatacca ctgccagcta
     4921 atcgaggtcg gatgactaaa tagatagtag aagctaaggc gacagtaata acagcattaa
     4981 ctgaagttgt aatagcgttc cagcttgcta aggtggcagc agccttttct ggagcaccta
     5041 aaatcatcga attataaaaa tagccaaaga ttggctctcc aaccacatta aaaaacattc
     5101 cagccataca agaaatgaca acataaatta ttaaatgaga atcattatca tgtttttcaa
     5161 ttttgaaaat tttatgtgca actaatccag taacgattcc aatcattaat ttaaggataa
     5221 ttgttttagg tgcataggta acataaacag gatcaagtag atcaccaatt cccattccaa
     5281 tcgctcctgc aattcctcca ggaagtcccc ctagcaataa tgcagctaag acacaaaagg
     5341 tatttcctaa atgaaatgat gtagttcccc ctggcgttgg tattttgatt tgtagaaaag
     5401 taaaagagac ataggctaat gcagccatca aaccaattgt cgctatttta agtgttttat
     5461 tattcataaa aatcccccta tttattaaat ctataagtat cttaacatta cactggatat
     5521 taaaaaagat acagttttta ataaatttat aaggacagta agaaaatttg caagttttag
     5581 gattgtacaa ttattaatgg ttaagttaca atataattat agtagtctag gaggtgtcag
     5641 gattgaaatt caaaaatatc gaagagttaa tggataaact aaataatgaa tataaagtcc
     5701 ttctagatgt tattgataat gttattctcg taattgataa tgaaatgaag atactttttg
     5761 tgaaccgaaa aggtcgtaag ctgataggtg aaaatgtatt aatgcagcct tgtaaagctc
     5821 ttaaaatgga taattgtagt acggaaaagt gctgtattat gcgttacctt cacggtttac
     5881 aaccattaga taatcttcat aaagatggaa gtgtagaaaa agtaacagtt tcaagatttt
     5941 atgataacca aaacaatccg caaggtttta ttatcgtagc taccgatata accgaattat
     6001 caaatatgaa aaaggagctt ctaataggtg aagaaattta taagcttgct ttaaaacaag
     6061 caaatacaac attgtggcaa tatgatgtgt taaatcatac aattgagcag ctattttgtc
     6121 ctgatgaagt tgcattagga attttagata ttaataaaac ttattataat atcccagaaa
     6181 gtttggttga agcaaagatc atcagtcaag aggatggttt acgagtaaga aaattatgcc
     6241 aagagattga aaaaggcagg cctgaaactt caattgaatt aaagatgaag cgtggagatg
     6301 gagaagaacg ctggatcagt ctaaaatgtt caacaatctt tgatgagcag ggacgtgcag
     6361 ttaaatcaat tggaatcggt aaagatatta cggattttgt tgaactaaag agcaagtatg
     6421 aaattgaacg agaatatcgt gaggctttag gtaaagatgc actgagttat attgaagtta
     6481 atttaacgat gaatgaagtt attgatcgga aaatagcaaa aaataatttt attgattttt
     6541 atgatggtaa aaactatgaa aaatcaattt taaaactaag tgaaaattca attcctagtg
     6601 agtatggcaa ag
//
LOCUS       DAOMTA010000054         6557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_190_length_6557_cov_2.597508, whole genome shotgun sequence.
ACCESSION   DAOMTA010000054 DAOMTA010000000
VERSION     DAOMTA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190_length_6557_cov_2.597508"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PLK68_02485"
     CDS             <1..1030
                     /locus_tag="PLK68_02485"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRM89309.1"
                     /translation="APKCVVLFSHLGKVKTEEDKAKNDLAVVAPCLEKHLGKPVTFVN
                     ATRGPVLEDAIKNAADGAVILVQNTRYEAGESKNDPELGKYWASLGDVFVEDAFGSVH
                     RAHASTAGIPAHLPSAAGFLVEKEIAYIGKAVNDPERPMVAILGGAKVSDKILVIENL
                     LKVADKVIVGGGMCYTFAKAMGHNIGNSLVEDDRIEIAKELIAKAGDKLILPIDSICS
                     DKFAVDGDIKECGEDVPDGYMGLDIGPKSVELFKEALQGAKTVVWNGPMGVFEMEPFA
                     KGTIAVCEALANLDGANTIIGGGDSAAAVMQLGYADKVSHISTGGGASLEYMEGKVLP
                     GIAAIDDK"
     gene            1046..1789
                     /gene="tpiA"
                     /locus_tag="PLK68_02490"
     CDS             1046..1789
                     /gene="tpiA"
                     /locus_tag="PLK68_02490"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRM89310.1"
                     /translation="MRKPIIVGNWKMNKTIAETKAFVEAVDAKVSDSADWGIATPYLA
                     LQAAKEGTKKLLVAAENCHFKDSGAYTGEVSVEMLKEIGVEWVILGHSERRQYFGETD
                     ETVNAKMLQVLKNDMTPIVCVGETLEEYEAGTTKDVVKTQTVAAFKDVCPKCAGRTVI
                     AYEPVWAIGTGKTATNEIAQDVCGYIRSVVAELYGQEVADQVRIQYGGSVKPEGLKTL
                     LEQPDIDGALVGGASLQADSYIAMIENLG"
     gene            1966..2349
                     /locus_tag="PLK68_02495"
     CDS             1966..2349
                     /locus_tag="PLK68_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HRM89311.1"
                     /translation="MKLLKCPICGNVVEMVEDHGVPLMCCGKKMEEVEAGAVDSALEK
                     HVPVLKVEGDCLTAVVGDVLHPMTPEHLISNIWIEFADGSNKKVTLTSDDEPIAKFNI
                     AGKSGKATVYEYCNLHGLWKTEIEL"
     gene            complement(2495..3256)
                     /locus_tag="PLK68_02500"
     CDS             complement(2495..3256)
                     /locus_tag="PLK68_02500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM89312.1"
                     /translation="MKTLQNKVAIITGGGRGIGFGLAKAFAKAGANLVITGRNRNTLH
                     NAKEKLESQYQINVLCIPADGANEDDVNNVINKTIKYYGKINIVVNNAQASKSGVMLK
                     DHTKEDFDLAINSGLYATFFYMRAAYPYLKESHGSVINFASGAGLFGKLGQSSYAAAK
                     EGIRGLSRVAAAEWGPDNINVNVICPLAMTEGLTKWKEEYPDLYEKTIQGIPMQRFAD
                     PENDIGSAAVFFASEAGHYITGETMTIQGGSGLRP"
     gene            3613..>4089
                     /gene="rplK"
                     /locus_tag="PLK68_02505"
     CDS             3613..>4089
                     /gene="rplK"
                     /locus_tag="PLK68_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018580273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRM89313.1"
                     /translation="MSKKVARVMKIQFPAGGAKPGPALAGAGIQMPKFCTAFNDQTRD
                     RMGETVPVVLTIYEDKDFSFVLKTAPAAEMIKKACGIKKGSSNAGTTEVATLSAEKLK
                     EIAEYKMPDLNAIDLESAMKIIAGTARNMGIKIEGFEPLTKLANELKMPQLATQVLQ"
     assembly_gap    4090..4099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4170..4856
                     /gene="rplA"
                     /locus_tag="PLK68_02510"
     CDS             4170..4856
                     /gene="rplA"
                     /locus_tag="PLK68_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRM89314.1"
                     /translation="MAKKSKRYQEAAKLIEKGKAYSIEEAVALVKETSKVKFDAAVDV
                     AFRLGVDPRQADQQLRGALVLPNGTGKSKKVLVVTEGPKAQEAKDAGADVVGGKEILE
                     DIKKGWLDFEVMIATPDMMAELGKLGRILGPKGLMPNPKTGTVTMDVAKAVKETKAGK
                     VTYRTDKEGNVQMTIGRVSFDNDKLVENFTAIYDLLVKIKPSTSKGVYMKNIVVSSTM
                     GPSIKIAAAK"
     misc_feature    4868..4992
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            5030..5629
                     /gene="rplJ"
                     /locus_tag="PLK68_02515"
     CDS             5030..5629
                     /gene="rplJ"
                     /locus_tag="PLK68_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRM89315.1"
                     /translation="MSAEAIKAKSALVEEIAAKLKGAQSAVVVEYRGLSVAEMTELRN
                     NLRAEDVELKVYKNSLVQRATVATEYEGLNAELTGPNAIALGNSDAVAPARVIAKFAK
                     DHEALVIKAAVVEGKLLTVDEVKEISKLPNREGMYSMLLGMLQAPVSKFARVVKAVAE
                     AKPEDGSAAAEEAAPVEEAAPEAPVVEETAEETKEETAE"
     gene            5659..6030
                     /gene="rplL"
                     /locus_tag="PLK68_02520"
     CDS             5659..6030
                     /gene="rplL"
                     /locus_tag="PLK68_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRM89316.1"
                     /translation="MAKLTHEDILAYLEEATILELNDLVKAIEEKFDVTAAAPVAVAA
                     AGAEEAAGEPANVTVTLTEAGGTKVAVIKAVREITGLGLVDAKGLVDKAPSVIKENIP
                     AAEGAEIKEKLEAAGASVEVK"
     gene            6085..>6557
                     /locus_tag="PLK68_02525"
     CDS             6085..>6557
                     /locus_tag="PLK68_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRM89317.1"
                     /translation="MKHYYTNNEDLISEPEQFIFTYRGKELIFTSDHGVFSKKMIDFG
                     SRVLLDAIELDEGKSTLLDVGCGYGTFGVALKSAYPALEIDMIDVNERALLLAKQNLA
                     ANNLEANVYLSSVYENVTNKYDVIVTNPPIRAGKETVTKILVEAKEHLNLYGEIWV"
BASE COUNT         2295 a          929 c         1351 g         1972 t
ORIGIN      
        1 agctccaaaa tgtgtagttt tattctcaca cttaggaaaa gtaaaaacag aagaagacaa
       61 agctaaaaat gatttagctg ttgtagctcc ttgtttagaa aaacatttag gaaaacctgt
      121 tacttttgtt aatgcaacaa gaggaccggt attagaagat gcaatcaaaa atgctgctga
      181 tggagcagta atcttagtac aaaatactcg ttatgaagct ggtgaatcta aaaatgatcc
      241 tgaattaggt aaatattggg cttctttagg tgatgtattt gttgaagatg catttggttc
      301 tgtacataga gcacatgctt caactgctgg tatccctgct cacttaccaa gtgcagctgg
      361 attcttagtt gaaaaagaaa tcgcttatat tggaaaagct gtaaatgatc ctgaaagacc
      421 aatggttgct attttaggtg gagctaaagt ttctgataaa atcttagtta tcgaaaactt
      481 attaaaagtt gctgataaag ttatcgttgg tggaggtatg tgctatactt tcgctaaagc
      541 tatgggtcat aacattggta actctttagt tgaagatgac agaattgaaa tcgctaaaga
      601 attaattgct aaagccggag ataaattaat cttaccaatc gattcaattt gttctgataa
      661 atttgctgta gatggtgata tcaaagaatg tggtgaagat gtacctgatg gatacatggg
      721 attagacatc ggacctaaat ctgttgaatt attcaaagaa gcattacaag gtgctaaaac
      781 tgtagtatgg aatggcccaa tgggtgtgtt cgaaatggaa ccatttgcta aaggaacaat
      841 tgctgtatgt gaagctttag ctaacttaga tggtgctaat actattatcg gtggtggaga
      901 cagtgctgct gctgttatgc aattaggata tgctgataaa gtttctcaca tttcaactgg
      961 tggtggagct tcattagaat atatggaagg taaagttctt cctggtattg ctgcaatcga
     1021 tgataaataa taaggggaaa ttataatgag aaaaccaatt atcgtaggaa actggaaaat
     1081 gaataaaaca atcgctgaaa caaaagcgtt tgttgaagct gtagatgcta aagtatcaga
     1141 cagtgctgat tggggtattg ctactccata cttagcttta caagctgcaa aagaaggaac
     1201 taaaaaatta ttagttgctg ctgaaaactg tcatttcaaa gatagcggag cttatactgg
     1261 tgaagttagt gttgaaatgt taaaagaaat tggagttgaa tgggtaatcc tagggcattc
     1321 tgaaagaaga caatattttg gtgaaactga tgaaacagtt aatgctaaaa tgttacaagt
     1381 attaaaaaat gatatgactc caatcgtttg tgttggtgaa acattagaag agtacgaagc
     1441 tggaactact aaagatgttg ttaaaactca aacagttgct gccttcaaag atgtttgccc
     1501 taaatgcgca ggacgtactg tgattgctta tgaaccagta tgggcaattg gaactggaaa
     1561 aactgctact aatgaaattg cacaagatgt ttgtggatat attcgtagtg ttgttgctga
     1621 actttacggt caagaagttg ctgatcaagt tagaattcaa tatggtggat cagttaaacc
     1681 agaaggatta aaaactttat tagaacaacc agatatcgat ggtgctttag taggtggagc
     1741 ttctttacaa gctgattctt acattgcaat gattgaaaac ttaggataat aattgagaga
     1801 cttcataaag aagtctcttt taatatgtat atttttattt aaatagggta taataaagaa
     1861 ggagttttat taattaaatt aataaagcat attagtaact attattgtta aactatgcta
     1921 tagtgtagtt gcaagggata gataaattaa aaaggaggtt tgaagatgaa attattaaaa
     1981 tgtccaattt gtggaaatgt tgtggaaatg gttgaggatc atggagtacc attaatgtgc
     2041 tgtggtaaga aaatggaaga ggttgaagca ggtgcggttg attcggcact tgaaaaacat
     2101 gttccagttt taaaagtaga aggtgattgc ttaactgctg ttgtagggga tgttttacat
     2161 ccaatgacac cagaacattt aattagtaat atttggattg aatttgctga tggtagcaat
     2221 aagaaggtca cactaacttc tgatgacgaa ccaattgcta agtttaatat tgctggtaaa
     2281 agtggtaaag ctacagtcta tgaatattgc aatcttcatg gattatggaa aacagaaatc
     2341 gaactataga aacctccatt gtggggtttt tatttttgtc cgtatataat aaaaagaaag
     2401 agtgttctgc tgatattatc tttactgtta gtctgggggt tccagtttta ggagtagtat
     2461 caagtgaatc actcttttta gtttaaaatt acgactaagg tcgaagacca ctaccacctt
     2521 gaatcgtcat agtttctccg gtaatgtagt gaccggcttc gctagcaaaa aagactgctg
     2581 cacttccaat gtcattttca ggatcagcaa agcgttgcat tggaatacct tgaatagttt
     2641 tttcatataa gtcagggtac tcttccttcc atttagtaag accttcagtc attgctaagg
     2701 ggcaaattac atttacatta atgttatcag gaccccattc agctgctgca acacggctta
     2761 accctcgaat cccttctttg gcagcggcat aagaagattg cccgagtttg ccaaatagtc
     2821 cagcccctga ggcaaagttg ataacactac catgactttc ttttaaatat gggtaagcag
     2881 ctcgcatata aaagaaggtt gcgtatagac cggaattaat tgctaaatca aagtcttctt
     2941 tagtatggtc ttttaacata acgccagatt tagatgcctg tgcgttattt acaacgatat
     3001 taatttttcc ataatatttg atggttttgt taatgacatt attaacatca tcttcattag
     3061 ctccatcagc aggaatgcat agtacattga tttggtattg agattctaat ttctctttag
     3121 cattgtgtaa agtatttctg tttctcccgg taataactag gttagccccg gctttagcaa
     3181 aggcttttgc taacccaaaa ccaataccac gaccaccacc agtaataata gcgactttat
     3241 tttgtaatgt tttcatatta tctctccttt agttagttat agctaactta tttgatatat
     3301 tagacgtatt tattaatgtt gtccaataat atgcaataat aatattcccg aaaacattta
     3361 ttttattaga tgaataagat aaatgtttta gtataaatct tatagtaaag gaaataaagc
     3421 aggttttatt attagatgat ctaagatatt tgaccataaa taaaaataat ttagagaaaa
     3481 aaataaaaaa gtattgcatt tatcgaataa taatgtataa tccttagttg acgcaatatg
     3541 tgttactatg cgtggaagga caatgtccat ttgaccacag cacggacaaa ttttatatag
     3601 gaggtatgtc acgtgagtaa aaaagtcgca agagtcatga agattcaatt cccagctgga
     3661 ggagctaaac caggtccagc attagcagga gcaggaattc aaatgcctaa attctgtaca
     3721 gctttcaatg atcaaacaag agatcgtatg ggtgaaactg taccagtagt tttgactatt
     3781 tatgaagata aagattttag ttttgtacta aaaactgcac cagcagcgga aatgattaaa
     3841 aaggcttgtg gaatcaaaaa aggttcaagc aatgccggaa ctactgaagt tgctacatta
     3901 tcagctgaaa aattaaaaga aatcgctgaa tacaaaatgc cagacttaaa cgctatcgat
     3961 ttagaatcag caatgaaaat catcgctggt actgctcgta acatgggtat taaaattgaa
     4021 ggatttgaac cacttacaaa attggcaaat gaacttaaaa tgccacaatt ggcaacgcaa
     4081 gttttacaan nnnnnnnnnt aattaaagat taaatattaa tcgtcatttg tggaaggata
     4141 atccgtttga ccactaagga ggaaaaataa tggcaaagaa aagtaaaaga tatcaagaag
     4201 ctgctaaatt aattgaaaaa ggtaaagctt attcaatcga agaagcagtt gcattagtaa
     4261 aagaaactag taaagttaaa tttgatgccg cagttgatgt tgcatttaga ttaggagtag
     4321 atcctcgtca agctgatcaa caattacgag gggcattagt attaccaaac ggtactggta
     4381 agtctaaaaa agttttagtt gttactgaag gacctaaagc tcaagaagct aaagatgctg
     4441 gagctgatgt tgttggtggt aaagaaattt tagaagacat caaaaaagga tggttagatt
     4501 ttgaagtaat gatcgctact cctgatatga tggctgaatt aggtaagtta ggtcgtattc
     4561 ttggacctaa aggattaatg cctaacccta aaacaggaac agtaactatg gatgttgcta
     4621 aagctgttaa agaaacaaaa gctggtaagg taacttaccg tacagataaa gaaggaaatg
     4681 ttcaaatgac aattggacgt gtttcatttg ataatgataa attagtagaa aactttacag
     4741 caatctatga tttattagta aaaatcaaac cttctacttc aaaaggtgtt tatatgaaaa
     4801 acatcgttgt ctcatcaact atgggaccta gcattaaaat cgcagccgct aaatagtagc
     4861 tgctaaacaa ggcgcaatca atatacccaa gacagtaact gggaaacctt aatttgttac
     4921 cgaggtgtta aagagatata tattatatat tgactttgac tctcgtttaa acgagagttt
     4981 tagtttgagt atattgttgc atatataaaa atttacagga ggtgtgacaa tgtcagcaga
     5041 agcaattaaa gcaaaaagcg cattagtcga agaaatcgct gctaaattga aaggagctca
     5101 atctgcagtt gtagttgaat atcgtggttt atctgtcgca gaaatgacag aattacgtaa
     5161 caacttacgt gcagaagatg ttgaattaaa agtttataaa aactcattag ttcaaagagc
     5221 tactgtagca acagaatatg aaggacttaa tgcagaatta acaggaccaa acgcaattgc
     5281 acttggtaat agcgatgctg tagcaccagc aagagtaatc gcaaaattcg cgaaagatca
     5341 tgaagcttta gtaattaaag cggcagtggt tgaaggtaaa ttattaacag tggatgaagt
     5401 taaagaaatt tctaaattac ctaaccgcga aggtatgtac tctatgttac ttggaatgtt
     5461 acaagcacca gttagcaagt ttgcacgcgt agtaaaagct gttgcagaag ctaaaccaga
     5521 agatggtagc gcagcagcag aagaagcagc accagttgag gaagctgcac cagaagcacc
     5581 agttgttgaa gaaacagctg aagaaacaaa agaagaaaca gctgaataat cagctatata
     5641 aattggagga aaataaacat ggcaaaatta acacatgaag atatcttagc ttacttagaa
     5701 gaagctacaa tcttagaatt aaatgattta gtaaaagcaa ttgaagaaaa atttgacgta
     5761 actgctgctg cacctgtagc tgtagctgct gctggagctg aagaagctgc tggagaacct
     5821 gctaacgtaa ctgttacatt gacagaagct ggtggaacta aagttgctgt aatcaaagct
     5881 gtaagagaaa tcactggatt aggattagtt gatgctaaag gtttagttga caaagcacca
     5941 tctgtaatca aagaaaacat tcctgctgca gaaggagcag aaattaaaga aaaattagaa
     6001 gctgctggag cttctgtaga agttaaataa tttatatttg actaaataaa gagcccttcg
     6061 gggctctttt atcttggagg acatatgaaa cattactata caaataatga agatttaatt
     6121 agtgaaccag aacaatttat ttttacttat cgtggtaaag aactaatatt tactagtgat
     6181 catggagttt tttctaaaaa aatgattgat tttggatcac gagttttatt agatgcaatt
     6241 gaattagatg agggtaaatc tactttattg gatgtggggt gcggttacgg cacttttggg
     6301 gttgctctta aaagtgctta cccggctttg gaaatcgata tgattgatgt gaatgaacgg
     6361 gccttgttat tagcaaaaca gaatctagct gctaataatt tagaagctaa tgtttattta
     6421 agtagtgttt atgaaaatgt tactaataaa tatgatgtaa ttgttacaaa tcctccgatt
     6481 cgggctggaa aagaaactgt tacaaaaatt ttagttgaag ctaaagaaca tttaaatctt
     6541 tatggagaaa tttgggt
//
LOCUS       DAOMTA010000055         6499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_197_length_6499_cov_2.265053, whole genome shotgun sequence.
ACCESSION   DAOMTA010000055 DAOMTA010000000
VERSION     DAOMTA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197_length_6499_cov_2.265053"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLK68_02530"
     CDS             <1..444
                     /locus_tag="PLK68_02530"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM89318.1"
                     /translation="ADNLALYAAISCGSELVITNKTGYDEEHVIKELIRNEIVYNKRH
                     AIVIASEKILDVEKLAQRISEETGFSGRSIVLGHIQRGGSPVPEDRILAARMANKAIE
                     LLQDNQSGKCVCLQDGKIVAKDIETALQEVNKSNQMLYQLFDDLV"
     gene            479..1264
                     /locus_tag="PLK68_02535"
     CDS             479..1264
                     /locus_tag="PLK68_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM89319.1"
                     /translation="MKKILFCDMDGTIIDLDGLLYPPDKEKVEKLHQAGHLFAFNTGR
                     NYEEALRSTNKLDLYYDYLVLNNGAHIVDRNDQELFKRVISKQAGIDIIEHCLKYDDM
                     WIYFYDGKKTLGYLRGQTYIYDEVGRSIVTNEHDFIKEYPNVEEFDIIAINQDNQQID
                     EVLKIQKYIDEHYADEAHGTLNMHYLDITPSGCTKGTGISNLVSLLNEDVVSYAIGDS
                     YNDISMFEHADHGYTFNRADELIKKHSDKQVDYLSELIDEMLK"
     gene            1276..2331
                     /gene="hisC"
                     /locus_tag="PLK68_02540"
     CDS             1276..2331
                     /gene="hisC"
                     /locus_tag="PLK68_02540"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRM89320.1"
                     /translation="MSWQDKLREVEPYVAGEQPKIVNMIKLNTNENPYGPSPKIKEVL
                     TSLDIDRLRLYPNSDAVDLRKELANYYGLEEEQVFLGNGSDEVLALIFLTFFNGKEPI
                     LFPDITYSFYPVYCELYQMAYKLVKLDRDFKINLNDFMQPNSGIIFPNPNAPTGLLVD
                     LDFIETILKNNPDSIVVIDEAYIDFGGTSCVSLIDKYDNLVVTQTYSKSRSLAGIRLG
                     IALGSKEAIRHLYDVKNSFNSYPVDYVAQQICLASILDDQDTKAKCAKVIKTREMTKT
                     RLKELGFIVPDSYANFVFVKHPDVEGQELFTALRQVGIIVRHWNTKRIDQYLRISIGT
                     DEEMEKMINFLEEYLNK"
     gene            complement(2359..3084)
                     /locus_tag="PLK68_02545"
     CDS             complement(2359..3084)
                     /locus_tag="PLK68_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HRM89321.1"
                     /translation="MKIIIAPAKVMKTKQVNIETTDILFKDKTTQLHNYLMKFNLDQL
                     HDIMKISFKMADTVYSYFHNEYTAVPALYCYQGTVFKQLSLKDYDADDFVYLEQYLNI
                     LSAYYGILKYNSAITPYRLDMIMKIDFNLYQFWQDEIDNYFKNEDYIISLASKEFTKM
                     LSHPHIINIDFVEDKAGKMVRNSMYVKQARGKMLDLMVKNKISDLDSLKRLTFDGYIY
                     HEELSTDDNYVYIRNGKQTYKKL"
     gene            3153..4160
                     /locus_tag="PLK68_02550"
     CDS             3153..4160
                     /locus_tag="PLK68_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HRM89322.1"
                     /translation="MNNTIIIDKILIHMLDLEHSKIIYSDTFINLTEGTTEYYDKKIE
                     KCLENSGIKELVTGSEHHLLQAGKKMIESNEAFKEESIKITQDLFDLCTKIEEMPNAN
                     LMFVELKVDGKKFVLIIKLNYKTMPMSLIEENDGIRSIRFINQQILPNKTTAVEEAII
                     INVEDNILSIIEKRYMIDGKPGYYLNEQYIKGEPKLTDKQKMSIVNKVVKKVDSEYNV
                     VEGDPLPLVKKELVDLVMDHRPVKPMELAKKVMGNDYNATEEVELIMRDLGIEEDDEI
                     VNVPVSLDRMSRCKLVLDDDRIIELNVEDYLEGVDIVKEMDEGGMTRIILKNIKDIVV
                     K"
     gene            complement(4185..>6499)
                     /locus_tag="PLK68_02555"
     CDS             complement(4185..>6499)
                     /locus_tag="PLK68_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM89323.1"
                     /translation="TFNLLPKTKRIDLKFNYRSNKIVLDSVNYIFNQIMDKDIGGLDY
                     YLDESAKLNYDFLRKEGAKELEDLPEVTAKASQRLDEEARFTSEVILSYQTGTISNDA
                     ELEAKIVAKKIQDMIGHLELDNFNGTKRLADYKDIVVLMRSTRSFIAFKKIFNKFNIP
                     SHIVLSQGFLQAPEIINLVNVLKAFNNRLDDIAFTSLLKGNYVISHFSENFLAQIKID
                     ETISMFDNCINYLEKKLDNYEALETFINYYQDMRNYFNSHSIKESLMKFLEDSNYLPF
                     LASLVNGPQRVANIELMIQKLDEMHDDSLNTITTKFDDMINNGVNLSPAMVSSNDDNV
                     VSFMTIHKSKGLEFPIVFVSNMQNKFNQQDARERIISDKKLGIAIKPRVKCDLEPYQD
                     VIVEYENKYRKVIATAQTSEAINEEMRIFYVALTRASQKLIMTGLIKEPQDLIKWQKY
                     VINNSEDSMINPRCKDKVILYHNARKQNSYLDWLGISLMSHSNIINQGLKKELLDHDQ
                     TDDLVNEIKQNFQTILIHQNKHLIQENTKHSKFSIKILSHHDVEEQIIVSNKSDTILD
                     LSSYHRYSNFEYPYPTNLEKSIAVTRKIEDGDRSFKDISYELDDSPVDAGTRGTIIHS
                     VLEHLDIDVHLDLSDNLNKLKQANLYDNEAWQLIDKYQQHLENFITSPVYQLMVNADY
                     LYREKEFSMLENGQIIHGIFDVVCIKDNQITIIDYKTDNLNKNTSKEILISLHKPQMD
                     YYKKILARVFPQANIQAIVYYLYINKYVTI"
BASE COUNT         2304 a          827 c         1106 g         2262 t
ORIGIN      
        1 gcagataatt tagctcttta tgcagctata tcttgtggaa gtgaacttgt tatcaccaat
       61 aagacaggct atgatgaaga gcatgtaatc aaagaattga ttaggaatga aattgtttat
      121 aataaacgcc atgcaattgt aatcgcaagt gaaaaaattt tagatgttga aaagctagca
      181 caacgaatca gtgaagaaac aggattttca ggtcgctcga ttgttttagg acatattcaa
      241 cgaggcggct caccggtgcc ggaagatcgg atattagctg caaggatggc aaataaagcg
      301 atagaacttc ttcaagataa tcaaagcggt aagtgtgtat gcctgcaaga tggaaaaatt
      361 gtggcgaaag atattgaaac agcgttacaa gaagtaaata aaagtaatca aatgctttat
      421 cagctctttg atgatttagt ctaaactgtt ataatatggc tatatattgg aggattttat
      481 gaaaaaaata ctattttgtg atatggacgg aacaattatt gatttagatg ggttattata
      541 tcccccggat aaagagaaag tggaaaagct tcaccaggct gggcacttgt ttgcttttaa
      601 tactgggaga aattatgaag aagcattacg cagtacaaat aaattagatt tatattatga
      661 ctatttagtt ttaaataatg gagctcatat tgttgatcgt aatgatcaag aattatttaa
      721 acgagttatt tcaaaacaag ctggtattga tatcatagaa cattgtttga aatatgatga
      781 tatgtggata tatttttatg atggaaaaaa gacattaggg tatttgagag gtcaaacata
      841 tatttatgat gaggtaggac gtagtattgt aacgaatgag catgatttta taaaagagta
      901 tccaaatgtt gaggagtttg atataatagc gataaatcaa gacaaccagc agattgatga
      961 agttttaaag attcaaaaat atatagatga acattatgca gatgaagcgc atggtacatt
     1021 aaatatgcat tatttagaca ttacgccaag tggatgtacg aaaggaactg gaattagcaa
     1081 tctagttagt ttacttaatg aagatgttgt aagttatgcg attggggact cgtataacga
     1141 tattagtatg tttgagcacg cggatcatgg ttacactttt aatcgagcgg atgaactgat
     1201 taaaaagcat agtgataaac aagtcgatta tttatcggag ttgattgatg aaatgcttaa
     1261 ataaggggga attatatgag ttggcaagat aaattgcgcg aggttgaacc atatgtagca
     1321 ggggaacagc ctaaaattgt gaatatgatc aagttaaata caaatgagaa tccttatgga
     1381 cctagtccta aaattaagga agtattaaca tctttggata ttgatcgttt aaggttatat
     1441 ccaaacagtg atgctgttga tttacgtaaa gaattagcta attattatgg acttgaagaa
     1501 gaacaagtat ttttggggaa tggttctgat gaggtacttg cgttgatttt tttaacgttc
     1561 tttaatggaa aggaaccaat tttattcccg gatattacat attcttttta tccggtatat
     1621 tgtgaattat atcaaatggc atataaactg gttaaattag accgtgattt caaaattaat
     1681 cttaatgatt ttatgcagcc taatagtgga attatttttc caaatcctaa tgcgccaact
     1741 ggtttgttag ttgatttaga ttttatagaa acaatattaa aaaataaccc tgacagtatt
     1801 gtggtcattg atgaagcgta tattgatttt ggtggaacta gttgtgtctc tttaattgat
     1861 aaatatgata atctagttgt aacacaaaca tattctaaat cacgttcttt agctggaatt
     1921 agactaggaa tagctttagg aagtaaagag gcaatcagac atttgtatga tgttaagaat
     1981 tcatttaatt cttatccagt tgattatgtc gcacaacaaa tttgtttagc gagtatcctt
     2041 gacgatcaag atacaaaagc aaagtgtgct aaagtaatta aaacaagaga aatgactaaa
     2101 acaagattaa aagaattagg ttttattgta cctgacagtt atgcaaattt tgtttttgta
     2161 aaacatcctg atgttgaagg ccaagaatta tttacagcat tgcggcaagt aggaataatc
     2221 gttcgtcatt ggaatactaa gcgaattgat caatacttaa gaattagtat cgggacagat
     2281 gaagaaatgg aaaaaatgat aaatttctta gaagaatatt taaataaata aaaatagatg
     2341 gcacgccatc tatttttatt atagcttttt atatgtttgt tttccatttc taatgtaaac
     2401 ataattatcg tctgttgata attcttcatg atatatatag ccatcaaacg ttagacgttt
     2461 taagctatca agatcactaa tcttattttt aaccattaaa tcaagcattt ttccccgagc
     2521 ttgtttgaca tacattgaat tgcgtaccat tttacctgct ttatcttcaa caaagtcaat
     2581 gtttatgata tggggatgtg atagcatctt agtaaattct ttagaggcta gggaaataat
     2641 atagtcttca tttttaaaat aattatcaat ttcatcttgc caaaattgat ataaattaaa
     2701 atcaatcttc ataatcatat ctaaacgata tggggttatt gcactattgt atttcaagat
     2761 gccataataa gccgataaaa tatttaaata ctgttctaag tatacaaaat cgtctgcatc
     2821 atagtctttc aaactaagct gtttaaaaac agtaccttga tagcagtata gtgcaggaac
     2881 cgcagtatat tcattgtgga aataactata gacagtatcc gccattttaa aagatatttt
     2941 cattatgtca tggagttgat caagattaaa tttcattaaa taattatgta gctgtgtagt
     3001 cttgtcttta aataagatat ctgtagtttc aatatttact tgttttgttt tcatcacctt
     3061 tgccggtgcg ataataattt tcattttaat caccaagatt attatatcgt atttcaacaa
     3121 aatatgatat acttaaccgt ggtgatgaaa gaatgaataa tacaattata attgataaga
     3181 ttttgattca tatgttggat ttggaacata gtaaaattat ttactcagat acatttatta
     3241 atttaactga aggaacaact gagtattatg ataaaaaaat tgaaaaatgt ttagaaaatt
     3301 ctggaattaa agaattggta accggtagtg agcatcactt attacaagct ggtaaaaaaa
     3361 tgattgaaag taatgaggcg tttaaagaag aatcgattaa aataacgcaa gatttatttg
     3421 atttatgtac aaaaattgaa gaaatgccaa atgctaattt aatgtttgta gaattaaaag
     3481 tagatggtaa gaaatttgtt ttaattatta agttaaacta taaaactatg ccaatgagtt
     3541 taattgaaga aaatgatggt atacgtagta ttagatttat taatcaacaa attttaccaa
     3601 ataaaacaac agctgttgaa gaagcaatta ttatcaatgt tgaagataat attttgtcga
     3661 ttattgaaaa acgttatatg attgatggga aaccaggata ttacttgaat gaacaatata
     3721 ttaaaggtga accaaaatta acagataaac aaaaaatgag tattgttaat aaagttgtaa
     3781 agaaagttga tagtgaatat aatgttgttg aaggggatcc cctaccatta gtaaaaaaag
     3841 aacttgttga tttagtaatg gatcatcgtc cggtaaaacc gatggaattg gctaaaaaag
     3901 ttatgggaaa cgactataat gcaactgaag aggttgaatt aattatgcgc gatctgggaa
     3961 ttgaagaaga tgatgaaatt gtcaacgtgc cagtatcgct agatcggatg tcacgttgta
     4021 aattagtttt agatgatgat cgaattattg aattaaatgt tgaggattat cttgaaggtg
     4081 tagatatcgt caaggaaatg gatgagggcg gtatgacgcg aatcatctta aagaatatta
     4141 aagatattgt tgtgaaataa aaatgggtta cggataaccc atttttaaat agttacatat
     4201 ttattaatat atagataata aacgattgcc tgaatattag cttgtgggaa aacacgagct
     4261 aatatttttt tgtaataatc catttgcggc ttatgtaaag aaatcagtat ttctttacta
     4321 gtgtttttat tcaaattatc agtcttataa tcgataattg ttatttgatt atctttaata
     4381 cagacaacat caaaaattcc atgaataatt tgtccgtttt caagcataga aaattctttt
     4441 tcacgataaa gatagtcagc attaaccatt aactgataaa ctgggctagt aataaagttt
     4501 tcaagatgtt gttgatattt atcaattaat tgccaggctt cattgtcata aaggtttgct
     4561 tgtttgagtt tatttaagtt atctgataaa tctaaatgaa cgtcaatatc aagatgttca
     4621 agcacactat gaataattgt tccacgtgtt ccagcatcaa ctggggaatc atcaagctca
     4681 taacttatat ccttaaaact tctatcgcca tcttcaattt tacgagttac agcaatggat
     4741 ttttctaggt tagttgggta tggatattca aaattactat atcgatgata gctgcttaag
     4801 tcaagaatag tatcactttt atttgaaacg ataatttgtt cttcaacatc atgatgacta
     4861 agaatcttga tagaaaattt actatgttta gtattttctt gaattaaatg cttattttga
     4921 tgaatcaaaa tagtttgaaa attttgtttt atttcattaa caagatcatc tgtttgatca
     4981 tggtctagga gttctttttt tagaccttga ttaataatat tactgtgact cattaaagaa
     5041 attcctaacc aatctaaata gctattttgc ttacgtgcat tatgataaag aattacttta
     5101 tctttacaac gtggatttat cattgaatct tcactattat taattacata tttttgccat
     5161 ttgataagat cttggggttc cttaattaag ccagtcataa ttagtttttg tgaagctcta
     5221 gtcaatgcaa cataaaaaat tcgcatttct tcgttgattg cttcactagt ctgtgctgtt
     5281 gcaattactt ttcgatattt attttcgtat tcaacaataa cgtcttgata gggttctaaa
     5341 tcacatttta ctcgcggttt gatcgcaatt cctaattttt tatcactaat gattcgttct
     5401 cgggcatctt gctgattaaa tttattttgc atattagaaa caaaaacaat tggaaactcc
     5461 aatcctttag atttatggat agtcataaaa gaaacaacat tatcatcatt acttgaaacc
     5521 attgcgggag agagattgac tccattattg atcatatcat caaatttagt agttattgta
     5581 tttaaagaat catcatgcat ttcatctaac ttttgaatca ttaattcaat atttgcaact
     5641 cgttgaggac cattgactaa tgaagctaag aaaggtaaat aattactatc ctcaaggaat
     5701 ttcatcaaac tttctttaat agaatgacta ttaaaatagt ttcgcatatc ttgataataa
     5761 tttataaatg tttctaaagc ttcataatta tctaattttt tttcaagata attaatacaa
     5821 ttatcaaaca ttgagatagt ttcatcaatt ttaatttgcg ctaaaaaatt ctcactaaaa
     5881 tgacttatta cataattgcc ctttagtaaa cttgtaaagg cgatgtcatc aaggcgatta
     5941 ttaaaagctt ttaaaacatt aacgagatta attatttctg gagcttgtaa aaaaccttga
     6001 gataaaacaa tatgactagg aatattaaat ttattaaata tctttttaaa agctataaag
     6061 ctgcgagtac tacgcattaa aacaacaata tctttataat ctgccagtct ttttgtgccg
     6121 ttaaaattat ccaattctaa gtgaccaatc atatcctgaa tttttttagc aacaattttt
     6181 gcttctaatt cagcatcatt gcttatagta ccagtttgat atgataggat gacttcgcta
     6241 gtaaagcgtg cttcttcatc aagacgttgt gatgctttgg cggtgacttc tggcaaatct
     6301 tctaattctt ttgcaccttc tttacgtaaa aaatcataat tcaatttggc actttcatct
     6361 aagtaataat caagaccgcc aatatcctta tccatgattt gattaaaaat ataattgaca
     6421 ctgtcaagga caattttatt tgatcggtaa ttaaatttta agtcaatgcg tttagtttta
     6481 ggtagtagat taaaagtaa
//
LOCUS       DAOMTA010000056         6497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_198_length_6497_cov_2.515834, whole genome shotgun sequence.
ACCESSION   DAOMTA010000056 DAOMTA010000000
VERSION     DAOMTA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198_length_6497_cov_2.515834"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>636)
                     /locus_tag="PLK68_02560"
     CDS             complement(<1..>636)
                     /locus_tag="PLK68_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89324.1"
                     /translation="AGNMEEAYKIITSENALPAICGRVCPQENQCEGKCVRGIKGEAV
                     GIGRLERFVADYHMANGKAPELDIKSNGIKVAIIGSGPAGITCAGELAKKGYEVTVFE
                     ALHKTGGVLSYGIPEFRLPKALVQKEVDSVAALGVKFETNVVVGRSITIDELQEQGYQ
                     GIFIGSGAGLPRFQNIPGENLNGVYAANEFLTRVNLMKGYEFPNHPTPVKIT"
     assembly_gap    637..646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(647..840)
                     /locus_tag="PLK68_02565"
                     /pseudo
     CDS             complement(647..840)
                     /locus_tag="PLK68_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793089.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
     gene            complement(840..1688)
                     /locus_tag="PLK68_02570"
     CDS             complement(840..1688)
                     /locus_tag="PLK68_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRM89325.1"
                     /translation="MYKIVKKEVLNDVVELMEVHAPYVAKKCEPGQFIILRVGEDGER
                     IPLTIADFDREKETITIIYQIVGYSTKELAKLNEGDELTDFVGPLGVPTVLHEAKHVI
                     GVAGGVGSAPLFPQLRELANRGVDVDVIIGGREAQYVLLADEFKKFCKNVYIATDDGS
                     LGTKGFVTNVLSDLIEKGESFDEVIAIGPVPMMKAVVNVTKPKNIKTSVSLNPIMIDG
                     TGMCGCCRVSVDGKIKFACVDGPDFDGLQVDFDELMLRQRMFKEEEHTVSENANRMCN
                     LMGGVK"
     gene            complement(1916..2482)
                     /locus_tag="PLK68_02575"
     CDS             complement(1916..2482)
                     /locus_tag="PLK68_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HRM89326.1"
                     /translation="MFEFLAEKITQVLETKNIIVRDRAIYKYGIETWISALASILLLL
                     VVGIIFNCILEVIIYEAVFFILRRFTGGYYCTTHFECMSLYIAIFTLYMVLREYLKVS
                     IVMVCIAIAISLIIIISLSPVQSNDQQLTELEQRKYHLYSTLLSIIVGISCIVLRFLN
                     IPVYSILTYSFSLLAILMLGGKVVNRRS"
     gene            2665..3369
                     /locus_tag="PLK68_02580"
     CDS             2665..3369
                     /locus_tag="PLK68_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM89327.1"
                     /translation="MYNIALIDDDQRILQIVQYKVKSIFNSVGIDPTITCFNNPQKLH
                     MDTYYDVLFLDIDMPSLNGIELAQNYLQNHDDTTIIFITNKYDLVFNAFSVHPFDFVK
                     KENLETGLIQPIKHLISKLNRDNRVISLQDKNGLTTIKCKKILYCESYGHICYIHTTD
                     RVIKTNKYKLSNIESIINCEDFYMINQSYLVHWKYVINIENKSTTLEDGTVLPISKRR
                     FKDSLASYKKYTFRNI"
     gene            3462..4253
                     /locus_tag="PLK68_02585"
     CDS             3462..4253
                     /locus_tag="PLK68_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89328.1"
                     /translation="MATNKIMFFDIDGTILSETTHTIPKSTVEGLQKAKEQGHLIFIN
                     TGRPFSSIDECIKELDPDGYVCGCGTYIRYHDEVLFSKTLSQERCFEVRDLIRKTNVE
                     GVLEGKNTVYFDQNIRHPFLKGVKERYEATPTFNLSTFDDPNLSFDKLAVWFDEHGDI
                     ETFKSEITKDFEYITRAEDFGEIVPLGCSKATGIQFLLDYFGLDKDDAYVFGDSFNDE
                     AMLRYVKHAIVMGNGEPELFKLAYYVTKDIEEDGIYHALQHLNLI"
     gene            4323..5483
                     /gene="tgt"
                     /locus_tag="PLK68_02590"
     CDS             4323..5483
                     /gene="tgt"
                     /locus_tag="PLK68_02590"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRM89329.1"
                     /translation="MLALRRMIMAAITYELKHVCKQSGARYGILHTPHGDVETPMFMP
                     VGTLATVKGISPEMLKEMHSQVVLANTYHLWLRPGEDVVEKAGGLHKFMNYNGPMLTD
                     SGGFQVFSLGKTRKIEEEGVKFKSIIDGSSLFLSPEKAIEIQNKLGADMIMSFDECAP
                     YPCTYEYMKNSMERTLRWAKRGKEAHKNTEKQALFGIVQGGEFPDLREQCAKELVAMD
                     FPGYSIGGTSVGEPKDVMYKMVDDTIKWLPEDKPRYLMGVGNPIDLIECAIRGIDMYD
                     CVLPTRVARHGAIMTSRGRLNINNEKFKYDFTPLDPECDCYACKNYTRAYIRHLHKCD
                     EIFGKTLLSIHNVNFLLKLASDIREAIKEDRLLDFKEEFLDKYGHDVYKRAF"
     gene            5485..>6497
                     /gene="queA"
                     /locus_tag="PLK68_02595"
     CDS             5485..>6497
                     /gene="queA"
                     /locus_tag="PLK68_02595"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRM89330.1"
                     /translation="MKISEFDFNLPEELIAQTPLDKRDTSRLMVLNRNQQTIEHKHFY
                     DIIDYLKPGDILVRNNTKVIPARLFGIKEETNGHVEVLLLKDQGNDMWECLVGNARIV
                     KIDTIITFGDGLLKAQCVEIKEEGIRVFKMIYEGIFYEILDQLGTMPLPPYIKEKLDD
                     QNRYQTVYAKIEGSAAAPTAGLHFTDEIIEKIKAKGIEILDVTLHVGLGTFRPVKVDD
                     VLEHHMHSEYYMIEPDVADKLNQAKQNGQRIIAVGTTSTRTLEANMKKYGKFTSVHEN
                     TDIFIYPGYKYEAIDCLITNFHLPKSTLLMLISAFASKEFIFKAYQEAIDEKYRFFSF
                     GDA"
BASE COUNT         2225 a         1076 c         1021 g         2165 t
ORIGIN      
        1 gtaattttaa ctggtgttgg atgattaggg aattcataac ctttcattaa gttgacccgt
       61 gttaaaaatt catttgctgc atatacacca tttaagtttt caccaggaat attttgaaaa
      121 cgtggtaagc ctgccccact accaatgaaa ataccttgat atccttgttc ctgaagttca
      181 tcaattgtaa ttgaacgccc aactacaaca ttagtttcaa atttaacacc taaagcagca
      241 acactatcta cttctttttg gactaaagct tttggtaaac ggaattcagg gataccataa
      301 gataatactc cacctgtttt atgaagtgct tcaaaaacag ttacttcata accttttttg
      361 gcaagttctc cagcacaagt aattccggct ggtccagaac caatgattgc aactttaatc
      421 ccgttactct taatatctaa ttcaggggct tttccattag ccatatgata atctgcaaca
      481 aatctttcaa gtcgcccaat accaactgct tcacctttaa tcccacggac acatttacct
      541 tcacattgat tttcttgcgg acaaacacga ccacaaatag ctggtaaagc attttcgcta
      601 gtgatgatct tgtatgcttc ttccatattt ccagcannnn nnnnnntgaa taaattcagg
      661 aattggtacg ccaactggac acccttgttt acatggctga tgtttacaat ttaaacaccg
      721 tgtagcttct tccattgcca tttctttagt gtatcctaaa gctacttctt caaaattctt
      781 atttcttaca tctggttctt gttctggcat tactaccttt gttaatgaca tgtttggcat
      841 tatttgacac ctcccattaa attacacata cgattagcat tttcactaac agtatgttct
      901 tcttccttaa acattctttg tcgcagcatt aattcatcaa aatcaacttg aagtccatca
      961 aaatcaggac catcaacaca agcaaattta atttttccat caacactgac acgacagcat
     1021 ccacacatcc ctgttccatc gatcataatt ggatttaaag atactgaagt ttttatattt
     1081 ttaggtttag taacatttac aactgctttc atcattggca ctggtccaat cgcaattact
     1141 tcatcaaagc tttcaccttt ttcaattaaa tcacttaata cattagtaac aaaaccttta
     1201 gttcctaatg aaccatcatc agttgcaatg taaacatttt tacagaactt tttaaattca
     1261 tcagctaata gtacatattg agcttcacgt cccccaatga ttacatcaac atcaacacca
     1321 cggtttgcta attcacgaag ttgcgggaat aatggtgcac taccaacccc accagctaca
     1381 ccgattacat gtttagcctc atgtaatact gttggaactc ctaatggccc aacaaaatct
     1441 gtcaattcat caccttcatt taatttagct aattctttag ttgaataacc aacaatctga
     1501 taaatgattg taattgtttc tttttcacgg tcaaaatcag cgattgttaa tgggattctt
     1561 tcaccatctt caccaacgcg taaaataata aattggcctg gttcgcactt tttagcaaca
     1621 tatggtgcat gaacttccat cagttcaacg acatcattta gtacttcttt tttgacaatc
     1681 ttgtacatac tatttccccc tacattattt tatactttca tattgtatct tgcttgaata
     1741 aaaaaaacaa tgataattcg tagttttttg taagattgta tacatatttt gatcatctct
     1801 cttttcacta ctaataaact atacgattat ctttaacttt aataccaaca acaaaaaaag
     1861 cgttagtaat aactaaccct tataaatatt taaataaatt aatttggaga ggcaatcaac
     1921 tgcgacgatt gactaccttt cctcctagca ttaggatagc aagtaaagaa aacgaatatg
     1981 ttagaattga ataaactgga atatttaaaa atcttaaaac aatacaggaa atacctacaa
     2041 ttatggatag caaggtacta tagaggtgat actttctttg ttccaattca gttaattgtt
     2101 gatcgttcga ttgtactggt gaaagactaa taataattat taatgatatt gcgatggcta
     2161 tgcaaaccat aacaatcgat acttttaaat attcccttaa taccatataa agagtaaaaa
     2221 ttgcaatata cagactcatg cattcaaaat gtgttgtgca ataatagcct cccgtaaatc
     2281 ttcttaaaat aaagaatacc gcctcatata ttattacctc tagtatacaa ttaaagatga
     2341 tccccacaac caacaataat aagatactag ctaatgcaga aatccacgtc tctatcccat
     2401 atttatatat tgcccgatct cttacaatga tattttttgt ttctaacact tgcgttatct
     2461 tctctgctaa gaattcaaac aaaatcttca cccctcgcta ttaaaattat cataatttaa
     2521 atgccctgtc cgcactttta caaattcggg ctatattttg acaaattcgg tcaaaatata
     2581 attttttagg ccaatttaaa gtatcctcag caactatttt attgattgtg tataataagt
     2641 aagagcattt aaaaggagat aattatgtat aatattgcac taattgatga tgatcaaaga
     2701 attttacaaa ttgttcaata taaagtaaaa agtattttta acagtgtcgg tattgaccct
     2761 actattactt gttttaataa tcctcaaaaa cttcatatgg atacatatta tgatgtgctg
     2821 ttcttagata ttgatatgcc atcattaaat gggattgaat tagctcaaaa ctatctccag
     2881 aaccatgatg atactactat tattttcatt actaataaat atgatcttgt ttttaatgct
     2941 tttagtgttc atccctttga tttcgttaaa aaagaaaatc ttgaaacagg attgatccag
     3001 ccaatcaaac atttaatttc taaattaaat cgtgataatc gtgttatatc attacaagat
     3061 aaaaatggtt taacaactat taaatgtaag aaaattcttt attgtgaaag ttatggtcat
     3121 atttgttata ttcacacaac tgatagagtt attaagacaa ataaatataa actatctaat
     3181 atcgaaagca ttattaactg tgaagatttt tatatgatca accagtctta cttagttcac
     3241 tggaaatatg ttatcaatat tgagaataaa agtacaactc ttgaagatgg taccgtgttg
     3301 cctattagta aacgtagatt caaagattct cttgcttcat ataagaaata tacttttaga
     3361 aatatttaat tagcggctta attaaatatt ttttctattt tagataatca attgttgata
     3421 aaagtaagtt attgtataat gcaattatca tggaggtaat tatggcaaca aataaaatta
     3481 tgttttttga tatagatggc acaatcttat cagaaacaac acacactatc cctaaaagta
     3541 cagttgaagg actacaaaaa gcaaaagaac aaggacattt gatttttatt aatacaggca
     3601 gacctttttc atcaattgat gagtgtatca aggaattaga tcctgatggt tatgtttgtg
     3661 gttgtggaac atatattcgt tatcatgatg aagtgttatt ttcaaaaaca ttgagtcaag
     3721 aaagatgttt tgaagttcga gatttgatta gaaaaactaa tgtcgaaggt gttcttgaag
     3781 gaaaaaatac cgtttatttt gatcaaaata ttcgtcaccc ctttttaaaa ggagtcaaag
     3841 agcgctatga agctacacca acttttaact tatcaacttt tgatgaccct aacctgtcat
     3901 ttgataaact agctgtctgg tttgatgaac atggagatat tgaaactttt aagtctgaga
     3961 ttacaaaaga ttttgaatat attactagag cagaagactt tggagaaatt gttcctttag
     4021 gatgctctaa agctacagga attcaatttt tactagatta ctttggtctt gataaagatg
     4081 atgcttatgt ttttggtgat agttttaacg atgaagcaat gcttagatat gttaaacatg
     4141 cgattgtgat gggaaatggc gaaccagaat tatttaaact tgcttattat gtaactaaag
     4201 atattgaaga agatggtatt taccatgctt tacaacattt aaatcttatt tagccctata
     4261 attttatagg gttttattat tgcaaactaa aattgtaaat agagcaagat tgcggtataa
     4321 taatgcttgc tttaaggagg atgattatgg cagcaataac ttatgaatta aaacacgttt
     4381 gtaaacaatc gggtgctcgc tatggaattt tacatacccc tcacggtgat gtcgaaactc
     4441 caatgtttat gcccgttggt acgctagcta ccgtaaaagg aatttcccca gaaatgttaa
     4501 aagaaatgca ctcacaagtg gtgttggcta atacttatca cctatggtta cgtcccggtg
     4561 aagatgttgt cgaaaaagct gggggactac ataaatttat gaattataac ggaccaatgc
     4621 taactgatag tggtggtttc caagtttttt ctctaggtaa aacacgaaaa atcgaagaag
     4681 aaggcgtaaa atttaaaagt attattgatg gaagttcatt gtttttatca ccagaaaaag
     4741 caattgaaat tcaaaataaa ctaggtgctg atatgattat gtcatttgat gaatgtgccc
     4801 catatccatg tacctacgaa tatatgaaaa atagcatgga acgaacttta cgatgggcaa
     4861 aacgtggaaa agaagcacac aaaaacacgg aaaaacaggc tttatttgga attgttcaag
     4921 gtggtgaatt ccctgatttg agagaacagt gtgctaaaga attagtcgcg atggatttcc
     4981 ccggttattc gatcggaggg actagtgttg gagaacctaa agatgtaatg tataaaatgg
     5041 ttgatgatac tatcaaatgg ttgcctgaag ataaaccccg ttatttaatg ggagttggca
     5101 atccaattga tctaatcgaa tgtgccattc gtggaatcga tatgtatgat tgtgtgttac
     5161 ctactcgcgt agctcgccac ggtgcaataa tgacaagcag gggacgttta aatattaata
     5221 atgagaaatt taaatatgat tttacacctc tagaccctga atgcgactgt tatgcttgta
     5281 aaaactatac tagagcatac attcgtcatt tacataaatg tgatgaaatc tttggtaaaa
     5341 cattactatc gattcataat gttaactttt tactgaagtt agcaagtgat attcgtgaag
     5401 caattaaaga agatcgctta cttgacttta aagaagaatt tttagataag tacggtcatg
     5461 acgtatataa gagggctttt taagatgaaa ataagtgaat ttgattttaa tcttcctgaa
     5521 gaactaattg cccaaacccc attagataaa cgcgatactt cacgtttaat ggtcttaaat
     5581 cgtaatcagc agacaattga acataaacat ttttatgata ttatagatta tttaaaacct
     5641 ggtgatatcc tagttagaaa taatactaaa gtaatacccg ctcgtttatt tggaattaaa
     5701 gaagaaacta acggtcatgt ggaagtccta ctattaaaag atcaaggcaa cgatatgtgg
     5761 gaatgtttag ttggaaatgc tcgaatcgtt aaaattgata ctataattac ttttggtgat
     5821 ggccttttaa aagctcaatg tgtcgagatt aaagaagaag gaattcgagt attcaagatg
     5881 atctatgaag gaattttcta tgaaatccta gatcaattgg gaacgatgcc acttccacct
     5941 tatattaaag aaaaactaga tgatcaaaat cgttatcaaa ctgtttatgc taaaattgaa
     6001 ggaagtgctg ctgctccaac cgcaggactt cattttactg atgaaattat tgaaaaaatt
     6061 aaagctaaag gaattgaaat ccttgatgta actttacatg ttgggttagg aacttttaga
     6121 ccggtaaaag ttgatgatgt tctagaacat catatgcaca gtgaatatta tatgattgaa
     6181 cctgatgttg ctgacaaatt aaatcaagct aaacaaaacg gtcaacgaat tatcgcagtt
     6241 ggtacaactt caacaagaac acttgaagct aatatgaaaa aatatggaaa atttacatct
     6301 gttcatgaaa atactgatat ctttatttat ccaggttata aatatgaagc aatcgactgt
     6361 ttgattacca actttcattt gcctaagtca actttattaa tgttgatcag tgcttttgca
     6421 agtaaagaat ttatttttaa agcttatcag gaagcaatcg atgaaaaata tcgtttcttt
     6481 agttttggcg atgcaat
//
LOCUS       DAOMTA010000057         6493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_199_length_6493_cov_2.295744, whole genome shotgun sequence.
ACCESSION   DAOMTA010000057 DAOMTA010000000
VERSION     DAOMTA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199_length_6493_cov_2.295744"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLK68_02600"
     CDS             complement(<1..1067)
                     /locus_tag="PLK68_02600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM89331.1"
                     /translation="MNENFNSAFYKKILDQINSNIYVTDVETDEIVYMNDYMKNTFQV
                     SNTEGKKCWQILQSGMNKRCEFCKIKQLQESNSQKICVWYEKNTITEKTYLNHDTLEL
                     WDNKLYHIQNSIDVTEQLQLSVEATIDELTGIFNRNAGKKRIEELLTAEYLNEDFVVV
                     LYDMNGLKWVNDTYGHLEGDRLLIFVAQNIKKRLKEPNFIFRLSGDEFIIVFLDTTVK
                     GVEVWMDNILLTLNQERIKENIDYDTSFSYGIANIHVDENLTVSDVLSIADSQMYIQK
                     RDYHILMNKKRLTNSQPKYNSIPLFEYNRDHLFESLSETIDDYLFVGNLKTSKFMYSR
                     QMMIDFRLPNQILDNAAAFWG"
     gene            complement(1405..2151)
                     /gene="pgeF"
                     /locus_tag="PLK68_02605"
     CDS             complement(1405..2151)
                     /gene="pgeF"
                     /locus_tag="PLK68_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRM89332.1"
                     /translation="MKLIKWNTVENIEAYTITKELGDMSYNNEDKQLILNNRKQLAKL
                     LKTDLEHMVAPHQTHSANFKEVSLKDGGRGMYCQDDAFNNTDALYTKDTDLFLLSFHA
                     DCTPVLLYCPKTKIIAAIHSGWLGTTKQIVSKVTKHLIENEHCDPKEMLAYIGPCICQ
                     DCLEVMDNVIDLVKQMDFDTTPYYKQTDTTHYLLDNKGLNRQMLLNLGLLKKNITVSP
                     YCTVENDKLFYSYRKYKDSGRNITIIKRQA"
     gene            2226..2618
                     /locus_tag="PLK68_02610"
     CDS             2226..2618
                     /locus_tag="PLK68_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89333.1"
                     /translation="MMFCNSGCGCNNCCGCGGCNSGMNCCRATPIVAPRKVCTSCSNQ
                     YVEQPIICPIECRHINNIVYYPRYYPRYERTFMTQSNGNPFASAANATPFNGAGTGFN
                     AANNTGDSVINGMYNNANNGGSGCGCNR"
     gene            complement(2640..4214)
                     /locus_tag="PLK68_02615"
     CDS             complement(2640..4214)
                     /locus_tag="PLK68_02615"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRM89334.1"
                     /translation="MVELTKSTKYELLIQVRNNRTNYKLLLSCHPMYARIQLTNLDYP
                     TPESPNPLTMLFRKHLEGGYIKKIEQIELDRICHFLFGCHNEFGDYVEYHCYIEIMGK
                     HSNIILCNQDKKILDCLKRITPNINSERFVQPGAMYQLPPMNQNKVDPFTSDFIENNN
                     LTKIYQGMSPILSKEILYRHDNGIDFKEIMDEIKNSDTLYISRVDEKEYFHLIPLTHL
                     NQQAQSYSLFDGLDKHFNLIDQKERIKQQTSNLLKFINNEYQKNVTKLAKLESTLEDS
                     NNSDEYRIIGDLLYSNLHLLKKGMRHVELDNYYDGSKIMVDLDEKLDPKSNAQKYYNK
                     YQKAKNSINVLHEQIDLTKKEIEYFDSLLTLMDNASYYDALEIKEELENLGYLKKKKK
                     TNTIRKNKKPSFETYYTKDGIEICIGKNNLQNDYLTFKHAHRYDTWFHVKDMPGSHVV
                     VKGDQLDEYTIRLASNIAAYYSKGKNSSSVPVNYTLIKTLKKPHGAKPGQVILDNYKT
                     IYIDPDQHCLDELQKK"
     gene            4399..4971
                     /gene="gmk"
                     /locus_tag="PLK68_02620"
     CDS             4399..4971
                     /gene="gmk"
                     /locus_tag="PLK68_02620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRM89335.1"
                     /translation="MQKGMLIILSGPSGVGKGTVREELFKDDSLNLAYSISMTTRKPR
                     PNERDGIDYFFVEEEEFKSKIEEGKLLEWAQFVGNYYGTPKDYVDQLLNEGKNVVLEI
                     EVQGALQVMEKCPDATTIFLVPPSLEELERRIRGRRTEEEEIVQQRLSKARKEIATKD
                     EYKYVVENDDVMAAKDKIAEIIKNHQTNQG"
     gene            5000..5410
                     /locus_tag="PLK68_02625"
     CDS             5000..5410
                     /locus_tag="PLK68_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89336.1"
                     /translation="MEKFKEYFKVFAYIFYPGMVLILYLAQGLEGKLGLDLIFCPTDI
                     IVLLAILGIYMLLDAFKVFDAKVFDFMKKNDGRLKVPFLLAFLLIIAVLMGLYDDLKV
                     YFYLNNDVSINLFIIIALGLGLAGIAFLLNKSEK"
     gene            5535..5951
                     /locus_tag="PLK68_02630"
     CDS             5535..5951
                     /locus_tag="PLK68_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HRM89337.1"
                     /translation="MLDVGLELNVLFDDPFWIGVFYLTNGDKCYVERVVFGQEPSDGE
                     IYVYFLNNYHKLNFVAEFKAKHKDKPKNPKRLQRMIRKQSMNLQTGTKSMQALKRQYE
                     QNKAKNKIIRREQRTAEKEHLFVLKQQKRKAKHRGH"
     gene            6001..>6493
                     /locus_tag="PLK68_02635"
     CDS             6001..>6493
                     /locus_tag="PLK68_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM89338.1"
                     /translation="MVKRVIIGFGILNLLAFLLVFKKPYKKQRYKKYDCAIICGYPAN
                     IDGEPSTIMKSRVEMGVTLYKKDKIKFLILSGGAVKNEYQEAKIMASYAISLGVKKED
                     ILIEGQSRSTYHNLMYSRDIMQINGLKNALVVTNSWHLRKADHYARKFKLDYAMVSAS
                     APKS"
BASE COUNT         2224 a          904 c         1081 g         2284 t
ORIGIN      
        1 tctccccaaa atgctgcagc attatcaaga atttgatttg gcaaacgaaa atctatcatc
       61 atctgacgtg agtacatgaa tttacttgtc tttaaatttc caacaaataa gtagtcatca
      121 attgtttctg ataaactttc aaaaagatga tcacgattat attcaaataa aggaatagaa
      181 ttatacttag gttgtgaatt tgttagcctt tttttattca taagaatatg ataatctcgt
      241 ttttggatat acatttgtga atcagcaata gataatacat cagaaacagt taaattttca
      301 tctacatgga tattagcaat tccataacta aaagaggtat cataatcaat gttttctttg
      361 attctttctt gatttaaagt taaaagaata ttatccatcc aaacttctac acctttaaca
      421 gtagtatcta aaaaaacaat tataaattca tcaccactaa ggcgaaagat aaagtttggc
      481 tctttcaaac gcttttttat attttgggca acaaatatta ataggcgatc gccttcaaga
      541 tgtccataag tatcattaac ccattttaaa ccgttcatgt catataaaac tacaacaaag
      601 tcttcgttaa gatattcagc agttaatagt tcttcaattc gttttttacc ggcatttcga
      661 ttaaaaatac ctgttaattc atcaattgtt gcttctacag ataactgtag ttgttcagta
      721 acgtcaatcg aattttggat atgataaagt ttgttatccc aaagttctaa agtatcatga
      781 tttaaatatg ttttttctgt tatcgtgttc ttttcatacc atacacaaat tttttgtgaa
      841 ttagattctt gaagttgttt gattttacaa aactcacatc ttttgttcat tccagattgt
      901 aaaatctgcc agcatttctt tccttcggtg ttagagactt gaaacgtatt tttcatataa
      961 tcgttcatat atacaatttc atcagtttca acatcggtga catagatatt agagttaatt
     1021 tgatcaagaa tcttcttgta gaatgctgaa ttaaaatttt cattcatcga caaaatcacc
     1081 tcaattatcg tttatatata ttacttttat tatatcattc atcgagaaac atataaaaaa
     1141 attaacaagt aaaaagataa gttaatgtag ttaaaatgtg attttgtaac tgatttataa
     1201 ccaatttatg taattttatg gaaaatttaa ttactaaaaa ataaatatta acaattaaag
     1261 tagaaaaaac tgatttaata aataaaaagg gctataacgc ctagagtgat atgcttcctc
     1321 ctaggtagac aaataaaata ataaaaactt gtcatctaga agggaacata tcaaagattg
     1381 aaaatcttta tttgttatag tcctttaagc ttgtcttttt atgattgtga tatttctgcc
     1441 actatcttta tatttacgat agctataaaa taacttgtca ttttcaacag tacaatatgg
     1501 cgaaacagta atattttttt tcaataaacc taaatttaat aacatttgtc ggtttagacc
     1561 tttgttatca agcaaataat gtgtagtatc tgtttgttta tagtacggag tagtatcgaa
     1621 atccatttgt ttaactaagt cgatgacatt atccattacc tcaagacaat cttggcagat
     1681 gcatggtcca atataggcaa gcatctcttt aggatcacaa tgttcatttt caattagatg
     1741 ttttgtgact tttgaaacaa tttgtttagt tgttcctagc caaccagaat gaattgcagc
     1801 aattattttt gttttaggac aatatagtaa cactggggta caatctgcat gaaatgataa
     1861 taaaaagaga tcagtatctt ttgtataaag agcatcagta ttgttaaagg catcatcttg
     1921 gcagtacata cctctgccac cgtctttaag agatacttct ttaaagttgg ctgagtgtgt
     1981 ttgatgtggt gccaccatgt gctctaaatc agtttttaaa agtttggcta attgtttacg
     2041 attattgaga atcaattgtt tgtcctcgtt attataagac atatcgccta attctttagt
     2101 aattgtatat gcttcaatgt tttcaacagt attccatttg attaatttca taatttagac
     2161 ctttctgtgt aatttgcctt agcaccaaat ttaatcgaac atatagtata ttaaaggagg
     2221 tggaaatgat gttttgtaac tcaggatgtg gttgtaacaa ctgctgcgga tgtggaggat
     2281 gtaacagtgg aatgaactgc tgccgtgcta ctccaatcgt tgcaccaaga aaagtttgta
     2341 caagctgctc taatcaatat gttgaacaac caattatttg cccaatcgaa tgtcgccata
     2401 ttaataatat cgtttattac ccacgttatt atccacgtta tgaacgtact ttcatgactc
     2461 aaagtaatgg aaatccattc gcaagtgctg ctaacgctac tccatttaac ggagcaggta
     2521 caggatttaa cgcagctaat aacacaggtg actctgttat taatggaatg tataacaacg
     2581 ctaataacgg aggaagcgga tgtggatgta accgctaaaa aagatagatg cccaggcatc
     2641 tatttttttt gtaattcatc gaggcaatgt tgatcaggat caatataaat agttttataa
     2701 ttatctaaga ttacttgccc aggtttagcg ccatgaggct ttttcaatgt ttttattaaa
     2761 gtataattga caggaacact actactgttt ttcccttttg aataataggc agcgatatta
     2821 ctagctaatc gaatcgtata ttcatctaat tgatcacctt taacgacaac atgactacca
     2881 ggcatatctt taacatgaaa ccaggtatca tagcgatgtg catgtttaaa agttagataa
     2941 tcattttgaa ggttattctt cccaatacat atttcaatac catctttagt ataatatgtc
     3001 tcaaaagaag gttttttatt tttacggatc gtattagttt tctttttctt ttttaaatat
     3061 cctagatttt ctaattcttc ttttatttct agggcatcat agtatgaagc attatccatt
     3121 agtgttaaaa gactgtcaaa atattcaatt tcttttttgg ttaaatcaat ctgctcatgc
     3181 agaacattga ttgaattttt tgccttttga tacttgttat aatatttttg agcattactt
     3241 ttaggatcaa gtttttcatc taaatcaacc ataatcttag aaccatcata gtaattatct
     3301 agttcaacat gacgcattcc tttttttaat aaatgtaagt ttgagtataa aagatcgcca
     3361 atgattcgat attcatcact attgttcgaa tcttctagag ttgattctaa ttttgcaagt
     3421 ttagtaacat ttttttgata ttcattatta ataaatttca aaagatttga tgtttgttgt
     3481 tttatacgtt ctttttgatc gattaaatta aaatgtttat ccagaccatc gaataatgaa
     3541 taagactgtg cctgctgatt tagatgagtt aatggaatta aatgaaaata ttctttttca
     3601 tcaactctac ttatataaag ggtatcgcta ttttttattt catccataat ctctttaaaa
     3661 tctataccat tatcatgacg ataaagaatt tctttagata ggattggaga catcccttga
     3721 tatatttttg ttagattatt gttttcaata aaatcactag tgaaaggatc aactttattt
     3781 tgattcattg gtggcaattg gtacattgcc cctggttgta caaaacgttc actattaatg
     3841 ttaggggtaa ttcgttttaa acaatcaaga atttttttat cttgattgca aagaataata
     3901 ttagaatgtt tacccataat ttcaatataa caatggtatt caacgtaatc accaaattcg
     3961 ttatgacagc caaaaagaaa atgacaaatt cgatctaatt cgatttgttc aatttttttg
     4021 atataaccac cctcaagatg tttacgaaat aacattgtta aaggattcgg actttccgga
     4081 gtaggatagt ctaaattagt cagctgaatt cttgcgtaca ttggatggca ggagagcagc
     4141 agcttgtaat tagttcgatt attacgaact tgaatcaata attcatattt ggtagatttt
     4201 gttaattcta ccaccagtta ttattttgtt taagtcatca attacttgat gcatcattat
     4261 tccatcatat gccatttttt atcaccgttg taagtataac atagattaaa aataattgac
     4321 attatatagt ttgaaaacaa ttgacaatat aagacgtatt ttgtatgatg aacttatcaa
     4381 aaaaaagggg tgtagacagt gcaaaaagga atgttgataa ttcttagtgg gcctagtggc
     4441 gttggtaaag gaacagtaag agaagaatta ttcaaagacg atagtttaaa tcttgcttat
     4501 tcgatttcga tgacaacaag aaaaccacgt ccaaatgaaa gagatggaat tgattatttt
     4561 tttgttgaag aagaagaatt taaaagtaaa attgaagaag gaaagttatt agagtgggcg
     4621 cagtttgtcg gtaattatta cggaacaccc aaagattacg ttgatcaatt attaaatgaa
     4681 ggtaaaaatg ttgttttaga aattgaagtt caaggggccc ttcaagtaat ggaaaaatgt
     4741 ccagatgcaa caacaatctt tttagtacca cctagcttag aagaactcga acgacgaatt
     4801 cgtggacgtc gtacagagga agaagagatt gtccaacaac gtttatcaaa agcaagaaaa
     4861 gaaattgcga ctaaagatga atataaatat gtagtggaaa atgatgacgt tatggcagca
     4921 aaagataaaa ttgctgaaat tataaaaaat catcaaacaa atcaggggta aatatccctg
     4981 atttttagta ggaggaaaaa tggaaaagtt taaagagtat tttaaggtgt ttgcgtatat
     5041 tttttatcca ggaatggttt taattttata tttggcgcaa gggttagaag gtaaattagg
     5101 gcttgattta attttttgcc caaccgacat tattgtgtta ttagctattt tgggaatata
     5161 catgttgtta gatgcattta aagtatttga tgctaaagtg tttgatttta tgaaaaaaaa
     5221 tgatggacgg ctaaaagtgc catttttact agctttttta ttaattattg cagttttgat
     5281 gggactatat gatgatttaa aagtatattt ttatttgaat aatgatgtaa gtattaattt
     5341 atttattata atcgcattag gtttagggtt agcaggaata gctttcctct taaataaatc
     5401 tgaaaaataa gttataatat ggttgctaag ataaaaacac gaacttgttg gtagtcaagt
     5461 tgtgcatttt gcatcagtaa cctgccctcc tgaggttgtc cgttcttaga agtaaatttc
     5521 aaggaggtag cgatatgttg gatgttggtt tggaattaaa tgttttattt gatgatccgt
     5581 tttggattgg agtattttat ttaacaaatg gagataagtg ttacgttgaa cgagttgtgt
     5641 tcggtcaaga gccaagtgat ggtgagatat atgtttattt tttaaataat taccataaac
     5701 ttaattttgt tgctgagttc aaagcgaaac ataaagataa accaaaaaat cctaagcgat
     5761 tacaacggat gattagaaaa caatctatga acttacaaac aggtactaaa tcgatgcaag
     5821 cgttgaaacg acaatatgag caaaataagg caaagaataa aattattcgt cgtgagcagc
     5881 gaacggctga aaaagagcat ctatttgtat tgaaacagca aaaacgtaaa gcgaaacatc
     5941 ggggccatta ggcccttttg ttacaatttc ccaggaaatg taaaataatg aggtaataaa
     6001 atggtcaaac gagtaataat tggatttgga atattgaatc tactagcatt tttattagta
     6061 tttaaaaaac catataaaaa acagcgttat aaaaaatatg attgtgcgat tatttgtggt
     6121 tatcccgcta atatcgatgg tgagccatca acaattatga aatcacgggt tgaaatgggt
     6181 gttactttat ataaaaaaga taagattaaa tttttaatct tatctggtgg ggcagttaaa
     6241 aatgaatatc aggaagcgaa aataatggct tcatatgcaa ttagtttagg ggtaaaaaaa
     6301 gaagatatcc ttatcgaggg acaatctcga agtacatatc ataatttgat gtatagccgc
     6361 gatataatgc agataaatgg attgaaaaat gctctagttg ttacaaatag ctggcattta
     6421 cgaaaggcag atcattatgc tcggaaattc aaattagatt atgccatggt cagtgcttca
     6481 gcaccgaaat cat
//
LOCUS       DAOMTA010000058         6426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_205_length_6426_cov_2.204050, whole genome shotgun sequence.
ACCESSION   DAOMTA010000058 DAOMTA010000000
VERSION     DAOMTA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205_length_6426_cov_2.204050"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3238
                     /locus_tag="PLK68_02640"
     CDS             <1..3238
                     /locus_tag="PLK68_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM89339.1"
                     /translation="ALSMAVTTVFSANISNVRALTNAEKARELVSKMTLEEKIGQKLM
                     LSFRSGWTMRDGTKISSVQTINDEIHEIIGEYDIGSVILFAANFNSDAKVNVELTDGL
                     QKAAMDKDLGKNSIPLLIATDQEGGIVYRLTGGTALPGNMALGASGNTENAVKAGNII
                     GSELNAVGVNVNFAPDADVNNNPNNPVIGLRSFSSNPQLAAKFVSAYIEGVQSNNVAT
                     AAKHFPGHGNVATDSHTGLPSVPATKEELYKTELVPFQAAIDAGTDMVMTAHIQFPNI
                     VTEEIYSDKKDELMSPPATLSREILTDLLRDEMKFDGVIVTDSMTMQGVANYFDTNER
                     NLLAVKAGVDILDIPFTDISSMKDMESKLIPLINAFVDAYTKEDGYNGIKLSLEELDK
                     SVERILVCKYNRDVMDLANDTTTLDDKKAIASKVVGSVENRETERLISANAVTVVKNE
                     NNVLPLKLTKDSKVVFASTYSRNNNRFILAWQRAKQAGIIPEGADYKILQHYNWTGLN
                     DKVNSAINSDGTNFVGTNKDVLDWGTVLVHASEISSASGIDGYLVACPQLFTKYCKEN
                     GKQTVVISLNHPYDVQAFPNADGILAVYGTTSLGLDITESFGGGTVGATAAFSPNLTA
                     GTEVALGTFGASGKLPVDIPKYVPKSGVYSTDENVYELGYGIEYDAIAKDPDKKGLAD
                     AIAQAKAIDKSVYTTESWEAAAEALNTLKVVLQTAENVNLTHKLAQSVVDENETDLRD
                     KYNTVLSLLVYKSDKKALSDLIAEAKKLDKDHYTIETWQNFEKALENAESIYDGNVPQ
                     DEVDLAKEALEKAKNELLEKNKKELSAALEMAKQVTPEQLDKVVPAVVTEFKAALENA
                     QIIFDKKDANELEINKAFERLSNVMQMLEFYKGDKTQLSALVEKIEKLNKNDYLSATW
                     KNLESVLTTAKDVLNNPDALEIEVSETHDQLVRTFLQLRLKPNKDLLSELINKAENTD
                     QTKYTAVSLLSLKSALVEAKAVLDNNEATQDEVTAVEILLKTALDNLVVKEITLDDPN
                     AQTPPDAKPGNGAITATKTGDDSMMLTFALAGLLALASSVIVLKKKEE"
     gene            3435..4226
                     /locus_tag="PLK68_02645"
     CDS             3435..4226
                     /locus_tag="PLK68_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRM89340.1"
                     /translation="MKQQKWNIHTHTTRCGHATGLDIQYIQSAIEAGFTMLGFSEHLP
                     YPEIRISGARMFYEQKDEYIATIRKLKQDYQDKIDIKVGYEVEYLKDHFNDLMNLKKE
                     CDYMILGQHFKYLIYDYDSYCSDEDVYVYAQQIEEALSKNFITYVAHPDYFMMGRRSF
                     SNACVDAAHRIAKASIRYDTPLEINLNGFGYGKKQYYINNELKACYPYPFREFWEIIA
                     MYGCKVTFGYDAHSPLTLLERERECWALDILSDLPLNFIDHIELK"
     assembly_gap    4686..4718
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5010..5774
                     /locus_tag="PLK68_02650"
     CDS             5010..5774
                     /locus_tag="PLK68_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM89341.1"
                     /translation="MRKYFFFDIDGTLTNSNPGGIILPSTFETLDKLRKNGHFVAIAT
                     GRAHWMAMDFSHESKIDNLVCDGGNGLVINGELLGIEPLDKNICLEIIDECIEKKFPF
                     GVSLGDVPELYTCNEWLSNFKMHTKIIVDPQIDFHRVDNIYKIFIMATHKQEAELTAI
                     HKLGYMRYHGDQLIVEPLEKYRGILKMIEIQGGKPEDIVVFGDGHNDISMMRQAPISI
                     AMGNAIDEVKEVATYITKSNQEDGIEYACKHFGWID"
     gene            5809..6063
                     /locus_tag="PLK68_02655"
     CDS             5809..6063
                     /locus_tag="PLK68_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89342.1"
                     /translation="MELIKWILMIAGILAFLIIGCMILLRSDEKKRSRTKQYQENIDM
                     LNETNEMLDTTLMELESYNEPLKKICEHIDNFNHKFLGQK"
     misc_binding    6114..6333
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     assembly_gap    6132..6141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2368 a          762 c         1261 g         1992 t
ORIGIN      
        1 ggctttatca atggctgtta caactgtatt tagtgcaaac atttctaatg ttagagcgct
       61 aaccaatgca gaaaaagcaa gggaacttgt cagtaaaatg acattagaag agaaaatcgg
      121 gcaaaagtta atgttatctt tccgtagtgg atggacaatg agagatggaa ctaaaatttc
      181 atcggttcaa actattaacg atgaaattca tgagattatt ggtgaatatg atatagggag
      241 tgttatctta tttgctgcta actttaattc cgatgcaaaa gtaaatgtag aattgacaga
      301 tggtttacaa aaagcagcaa tggacaaaga tttaggtaaa aacagtattc ctctattaat
      361 tgctactgat caagagggag gaatcgtata tcgtttaact ggtgggacgg ccttaccagg
      421 aaatatggct ttaggtgcta gcggtaatac tgaaaatgct gttaaagcag gtaatattat
      481 tggtagtgaa ttaaatgcgg ttggtgtaaa tgttaatttt gcgccagatg ctgacgtaaa
      541 taataaccct aataaccccg ttattggatt acgttcattc agtagtaatc cgcaattagc
      601 agctaaattt gtttctgctt atattgaagg agttcaaagc aataatgtgg caactgccgc
      661 taaacatttt ccagggcatg gaaatgtagc tacagattcg catacaggtt taccttcggt
      721 tcctgcgact aaagaagaat tatataaaac agagttagta ccttttcagg cggcaattga
      781 tgctggaaca gatatggtta tgactgctca tatccaattt ccgaatattg taacagaaga
      841 aatttactca gataaaaaag atgagctgat gagtccgcca gctacattat ctagggaaat
      901 tttaactgat ttattaagag atgaaatgaa gtttgacggt gtcattgtaa ctgattctat
      961 gacaatgcaa ggtgtggcta attactttga tacaaacgaa agaaacttat tagctgttaa
     1021 agctggagtt gatattctag atattccatt tacggatatt tcttctatga aagatatgga
     1081 atctaaatta attccactga ttaatgcatt tgtcgatgct tatactaaag aagatggtta
     1141 taatggaatt aaattatcat tagaagaatt ggataaatct gtggaaagaa ttttagtttg
     1201 taaatacaac agagatgtca tggatttagc aaatgataca actacattag atgataaaaa
     1261 agcaatcgct tcgaaagtgg ttggttctgt tgaaaatcgt gaaacagaga gattaatttc
     1321 tgcaaatgca gttactgttg ttaaaaatga aaataatgta ttaccactta aattgactaa
     1381 agattcaaaa gtagtatttg catcaactta ttcaaggaat aataatcgtt ttattttggc
     1441 ttggcaaaga gctaaacagg cgggaattat tccagagggg gcagattata agattttgca
     1501 gcactataat tggacaggat taaacgataa ggtaaacagt gctataaatt ctgacggaac
     1561 taattttgtt gggacaaata aagatgtgct agattggggt actgttcttg tccatgcctc
     1621 agaaatcagt agtgctagtg gaatagatgg atatttggta gcttgcccac aattatttac
     1681 taagtattgt aaagaaaatg gaaagcagac ggttgttatt tcattaaatc atccttatga
     1741 tgttcaagcc ttcccaaatg ctgatggtat tttagcagtg tatggaacta ctagtcttgg
     1801 gctggatatt acggaatcat tcggtggtgg tacagttgga gcaacagcag catttagtcc
     1861 taacttgaca gcaggtactg aagtagcttt aggtacattt ggagcttctg ggaaactacc
     1921 ggtagatatc ccaaaatatg ttccaaaatc tggagtatat tcaactgacg aaaatgtcta
     1981 tgaattagga tatggtatag aatatgatgc aattgctaaa gatccagata aaaaaggact
     2041 agcagatgcg attgcacaag ctaaagctat agataaaagt gtatatacta ctgagtcttg
     2101 ggaggcagct gcagaagctt taaatacttt aaaagtcgta ttgcaaacgg ctgaaaatgt
     2161 taatttgact cataaattgg cacagtctgt tgttgatgaa aatgaaacag atttacgtga
     2221 taaatataat acggttttat cattattagt atataaatct gacaaaaaag ctttatcaga
     2281 tttgattgcc gaagcgaaga aattagataa agatcattat actattgaaa cttggcaaaa
     2341 ttttgaaaaa gcattagaaa atgctgaatc tatatatgat ggaaatgtac cacaagatga
     2401 agttgattta gctaaagaag cattagaaaa agcaaaaaat gagttacttg agaaaaataa
     2461 aaaagaatta tcagctgctc ttgaaatggc taaacaagta acaccagaac agttagataa
     2521 ggttgtacca gctgtggtga ctgaatttaa ggctgcattg gaaaatgctc aaataatttt
     2581 tgataaaaag gatgcaaatg aacttgaaat aaataaagct tttgaacgct tatctaatgt
     2641 aatgcaaatg ttggaattct ataagggcga taaaacacaa ttaagtgcat tagtagaaaa
     2701 aattgaaaaa ttaaacaaaa atgattattt aagcgcaaca tggaaaaatt tagaatccgt
     2761 cttaacaacg gcaaaagatg ttttgaacaa tcctgatgct ttagaaatag aagtaagtga
     2821 aacgcatgat caattagtaa gaaccttttt acaattaaga ttaaagccaa ataaagattt
     2881 gttaagtgag ttgatcaata aagctgaaaa tacagatcaa acaaaatata cagcagtatc
     2941 attattatcg cttaagagtg cattagtaga agcaaaagca gttcttgata ataacgaagc
     3001 aacccaagat gaagtaactg ctgtagaaat attattgaaa acagcattag acaatttggt
     3061 agtgaaagaa attactctag atgatccaaa tgctcaaaca ccaccagatg ctaaaccagg
     3121 taatggtgct ataactgcga caaaaacagg tgatgattct atgatgttaa catttgcctt
     3181 agcgggatta ttggctttag ctagcagtgt gattgtattg aagaaaaaag aagaataatt
     3241 tatacttaag ctaaaaagtc actgtatata gtggcttttt ttgttttggt ttgttttgag
     3301 aaacaggatg atttatttaa taagaaatat gttataatat tcctaatatt attttatagt
     3361 acattggttt aaacaatatt tatttaaaat actaaataaa tttcacattt attagttaga
     3421 aaggggaatt tttaatgaag cagcaaaaat ggaatattca tacgcataca acaagatgtg
     3481 ggcatgcaac tggactagat attcaataca ttcaaagtgc aattgaggct ggatttacaa
     3541 tgttaggatt tagcgaacat ctgccttatc ctgaaatacg gatttctggg gcacgaatgt
     3601 tttatgagca aaaagatgaa tatattgcaa cgataagaaa attaaaacaa gattatcaag
     3661 ataagattga tattaaagtt ggctatgagg ttgagtattt aaaagatcat tttaatgatt
     3721 tgatgaattt gaaaaaagaa tgtgattata tgattttggg acaacatttt aaatatttaa
     3781 tttatgacta tgacagttat tgttctgatg aggatgtata tgtttatgca cagcaaatag
     3841 aggaggcatt atctaaaaat tttattacat atgtagctca tcctgactat tttatgatgg
     3901 ggagaaggag tttttcaaat gcttgtgttg atgcggcgca tcgaatagca aaagctagca
     3961 tccgttatga tacaccgtta gaaattaatc taaatggttt tggatatgga aaaaaacaat
     4021 attatataaa caatgaatta aaggcatgtt atccatatcc gtttcgtgaa ttttgggaga
     4081 taattgctat gtatggttgt aaagtaactt ttggatatga tgctcattca ccacttacgt
     4141 tattagaacg tgagcgggaa tgttgggctc tagatatttt aagtgatcta ccacttaatt
     4201 ttattgacca tatcgaatta aaatgagctt tatagataaa atattattgc taaaaattat
     4261 tattaactat attttttgat tcaaataaga ttagtaattt aagaaaaagg agattagaaa
     4321 tgttgtactc aaagaagata aatatggcag atattagagc aagaatatga gtatgtttct
     4381 aatgttctag aagacaaaaa tgattttgaa aataaaattt taccaggtta tattaattct
     4441 tccaacggga ttggacttct tgagggatgg gttccggaaa cagaagtgtt tttatggaag
     4501 atgatcaaat tattgactga tttaggattg gacattactt gactgaaaag ttggctcatg
     4561 gtgccggaca tattggatat gtaataaaaa agaatataga ggaaacgggc atactgtgaa
     4621 ggattaaagg taactcttca aatagctaaa aatattatta aagaggataa aatatatctg
     4681 tcagtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ttcaataaaa aaatggtgca
     4741 tatattcatc atgaagacga taagaaattt tatacacgaa taaaaaatca gtttaaagct
     4801 actctataat taattaaata ctagtaaagc cggctaaaaa caatgtacaa taaataatgg
     4861 gttattttta aatactaata aataacttga attaaaaaat aactatattt taatttggaa
     4921 aggaaggtat gcatgtctgt taattgctag tttagcaaag ctaggcaatt gagattatta
     4981 gttgcgtact aaagggagtg gaattatata tgagaaaata tttctttttt gacattgacg
     5041 gaacattaac taatagtaat cctgggggaa ttattttacc ctcaactttt gaaacattag
     5101 ataagttaag aaaaaatggt cattttgttg caattgcgac aggaagagcg cactggatgg
     5161 caatggattt tagtcatgaa tcaaaaattg ataatctagt ttgtgatggg ggaaatggtt
     5221 tagtcataaa tggagaatta ctaggaattg aaccattaga taaaaatatt tgtttagaaa
     5281 taattgatga gtgcatagaa aagaaatttc ctttcggtgt atctttaggg gatgttcctg
     5341 aactatatac atgtaatgag tggctaagta attttaaaat gcatacaaaa attattgtag
     5401 acccacagat tgattttcat cgggttgata atatttataa gatttttatt atggctacac
     5461 acaagcagga agctgagttg actgcaatcc ataaacttgg ttatatgcga tatcatggtg
     5521 atcaattaat tgttgagccg ttggaaaaat atcgtggaat tttgaaaatg attgaaatac
     5581 aaggaggaaa acctgaagat atagttgttt ttggtgatgg acataatgat atttcaatga
     5641 tgcgacaagc acctatttca attgcaatgg gaaatgcaat tgatgaggta aaagaagtag
     5701 ctacatatat aactaaatct aatcaagaag atgggattga atatgcttgt aaacattttg
     5761 gatggataga ttagctaaat gtggtatact actagtaggt gatgaaaaat ggaactaata
     5821 aaatggattt taatgatagc aggtattttg gcttttttaa ttattggctg tatgatattg
     5881 ctacgtagtg atgaaaaaaa gcggagtcga actaaacagt accaagaaaa tattgatatg
     5941 ctcaacgaaa ctaatgaaat gttagatact acattaatgg aattagaatc ttataatgaa
     6001 ccgctaaaga agatatgtga acatattgat aactttaatc ataaatttct tggtcaaaaa
     6061 taaccgaaaa cagttgacat aagattattt tttcatataa tacaggtata tataaaaact
     6121 cgttgataaa gnnnnnnnnn ngagtaattt aattaagttt cttagcgaac tggcgttggt
     6181 gaaaggtcag taaacaagat taaatgaacc gcactttgaa gaggactgtt tgaactaata
     6241 gtagggcagt cagtatgtct actttacaga caagagggga tataagactt tttatatcaa
     6301 caagagtggt accgcggaaa tttcgtctct tagcttttaa ttagctaaga gacttttatt
     6361 ttacgcaagg ataggaaagg aaaggtaaga aaaatgaaga aattattaaa agtattatta
     6421 gtatgt
//
LOCUS       DAOMTA010000059         6355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_206_length_6355_cov_2.242857, whole genome shotgun sequence.
ACCESSION   DAOMTA010000059 DAOMTA010000000
VERSION     DAOMTA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206_length_6355_cov_2.242857"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLK68_02660"
     CDS             complement(<1..264)
                     /locus_tag="PLK68_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89343.1"
                     /translation="MLNECKKDTFISDNILSYDKYMDLIDLLGGIESDEDGSQFVKVQ
                     VDKMIKYFLVPVSRGDKLSKEIVKEYIEKNAINKLEKEIENDNE"
     assembly_gap    281..290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<291..520)
                     /locus_tag="PLK68_02665"
     CDS             complement(<291..520)
                     /locus_tag="PLK68_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89344.1"
                     /translation="MENVVNSEYFKLAVVLKSVLGITHHEMKDIRENISKYKKRKEIV
                     QEINDYLNDDEISIFLPDNYKQQAQRLVLIFN"
     gene            complement(567..>1015)
                     /locus_tag="PLK68_02670"
     CDS             complement(567..>1015)
                     /locus_tag="PLK68_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRM89345.1"
                     /translation="ELKFLDREILMTLNMNSKNKIINAHVCSIGSANMGIVDPKSVFK
                     SALLSGANRVMIIHNHPSGDCTPSQEDIDVTNLLIKGGRILEIEVLDHIVIGENEYYS
                     IVGDRQEKYTEINNTDNLQNSYESLELCENNDKKIWLEEDVTEMII"
     assembly_gap    1016..1025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1400..2188)
                     /locus_tag="PLK68_02675"
     CDS             complement(1400..2188)
                     /locus_tag="PLK68_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRM89346.1"
                     /translation="MTDIEKLKKELIEQKQSKFPGNIYHVSQVLFAYNSNKIEGSRLT
                     EEQTEMIFETNSFFPDKDEKVVRVDDITETVNHFKLFDFILDHIDDRLNKDMIIEMNK
                     ILKKGTSDESNPRYNVGGFKIHPNVIGLVNVIKTSKPDEVERDIDVLIEEYNRLGKVT
                     LEDIVDFHLRFERIHPFGDGNGRVGRAIMFKECLKNNIVPFIILDRDKPYYLRGLKEY
                     DRDRTYLFETCLNEQDIYTDLCKQLLNLDFNITVDNEQDEDMEL"
     gene            complement(2420..>2974)
                     /locus_tag="PLK68_02680"
     CDS             complement(2420..>2974)
                     /locus_tag="PLK68_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HRM89347.1"
                     /translation="NERAIHYLTNERKLSDSLVSKLIEDGIVYEDIYHNVVFVGYDVN
                     NIKEKKPVFAIRRGTGNSKFHIDCKGSFKNNGLFYQNFEGIKKIVVTESVIDGLSYIS
                     LNRNNLTAHVLMCSGVGTMLNTLKYNLKNNPALKNLEEIEIMPDLDDAGVIAGNKIKK
                     WYDNEGVFNDNGNTVSLEIKNIRL"
     assembly_gap    2975..2984
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2985..3392)
                     /locus_tag="PLK68_02685"
     CDS             complement(<2985..3392)
                     /locus_tag="PLK68_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89348.1"
                     /translation="MTNVKILSNSEKEYRKTIVSEMLKELTCIEVATNVFNLTVHKSS
                     RSQRYLKPVEHSSMVFDILKNKVYWNARCGSVPLNVIEFYSEYTNTSTYDAINILLEY
                     FYSRNPNEIEQYLYDFSMDESHISQGLVLTQKND"
     gene            complement(3491..4498)
                     /locus_tag="PLK68_02690"
     CDS             complement(3491..4498)
                     /locus_tag="PLK68_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89349.1"
                     /translation="MFKFKKMTNILTEKEAKKIANDFYSRNNSSMFKKHDIFLECWIE
                     ETMDNQKFKIELHTIVSPNYDEYGECEFTSYYDSYKELLEKNIYCHIYRALTDGATTV
                     TPAIENPNNLLMSRIFSPIKIYINSQIVLPVKIMEQLGCHDWINDYYRNKANMEFINV
                     KLGKDKYTKLASQEDFDSAINNYFKEYLKKNNIKLSLKQEKNIGTEFKKLLNDDNFKC
                     ILGLFVTLDEQYCFESFFEKNNKTGIERIIIEHPEESEKFHHGYRWSALYDFIDNLRI
                     NSDEKALIIENREMLLDIYEHYPNHLQTLNHEIRKSYLSDEFETHFRYTEYEFVDERE
                     L"
     gene            complement(4744..>6355)
                     /locus_tag="PLK68_02695"
     CDS             complement(4744..>6355)
                     /locus_tag="PLK68_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRM89350.1"
                     /translation="FFNIPELMNSFREVAEIQTESMLNNALENSKLQRKRAIPPKHVG
                     DKAKVVSIEPSPELEDYIANVVERTEAIHNSAVAPYEDNMLKVTSDSKKASIDLRLID
                     ESYGLTPDNKLTVISEQIYRIYDEYRDQKATQLVFCDSSTPKSQFNVYDEIKNNLIKL
                     GVNDEEIAFIHDYKTTLKKEKLYRDVNRGEVRVLIGSTAKLGAGTNVQQRMIAIHHVD
                     VPWRASDIAQRNGRVFRQGNMNNEVYEFRYATKKSFDAYSWQLIETKSTYMNQLLEGS
                     GCAREIEEDTKATFSYAEIKAIASGNPLIREKLEVDTRLKTLESSLARHRKAIRKSQD
                     DKRNMPEKIKNLEMKFDRISKDYSVFKKHINFEYEMINEKMDIDFKVEYKDKVFTDLK
                     EYGDFIIRLSDMNKEKNSIPIGKMMGFEFFLVFNVGNGWNICFKGANNYLYNAGLLNA
                     VGRVNVGRMIRKIFEIKKDYEKYFIQINNMKENYAKLDDIINKKFESQEELLSLRQRQ
                     KEITNLLTDNNNGVVLEENEAEEIELEI"
BASE COUNT         1879 a         1165 c          700 g         2581 t
ORIGIN      
        1 ttcattatca ttttctattt ccttctctaa cttatttata gcatttttct ctatatattc
       61 tttaacaatt tcttttgaaa gcttgtcacc cctgcttact ggaactaaaa agtatttgat
      121 cattttatcg acttgtactt taacaaattg actgccatct tcatcacttt ctattccacc
      181 taacaaatca ataagatcca tgtatttatc ataagataga atattatctg atataaaagt
      241 atctttttta cattcattca acataaaatt gtcatcatag nnnnnnnnnn ttattaaaaa
      301 taagaactaa tctctgcgcc tgctgtttat aattatcagg taaaaagata gagatttcat
      361 catcattaag ataatcatta atctcctgga ctatttcttt tcttttttta tatttagaaa
      421 tattttctct gatatctttc atttcatgat gagtaattcc taatacactt tttaacacaa
      481 ctgctaattt gaaatactca ctatttacga cattttccat ttttacctcc ttctgcaaag
      541 taaaaaagag gcaaaatgcc tcttttttaa attatcattt ccgtcacatc ttcttctaac
      601 caaatttttt tgtcattatt ctcacacagc tctaaagatt cataactgtt ttgtaaatta
      661 tctgtattat ttatttcagt gtatttttct tgacgatccc ctacaataga ataatattca
      721 ttctcaccta tcacaatatg atcaaggact tctatttcta aaatgcgccc tcccttgatt
      781 aataaatttg tcacgtcaat atcctcctga cttggagtac agtcaccaga aggatggttg
      841 tgtataatca ttaccctatt tgctcctgat agtagagctg atttaaagac actttttgga
      901 tcaactattc ccatatttgc acttcctatc gagcatacat gagcattaat aattttattt
      961 ttactattca tatttaaggt cattaaaatt tctcgatcta aaaactttaa ttcatnnnnn
     1021 nnnnnccagc ggtgttctta tactcttttc acataaaaac tctctttcct taactaatct
     1081 gattccaaca acatcaatac tgttcatttg ctctcttcac cttttttatt ccattttcat
     1141 ctataacaat ctcctcatcg tcctttatat tatctatcag ttcctttaaa atactgtaac
     1201 taattttttt catcctgttc tccttgttat aattctctct cattttcatc ttcttcagca
     1261 tagttttcac gagagattga tgtatttttc atcaagttcc tcgattatat attcatgctt
     1321 cacatttatt agctccttct ttataaaata gtactgtatg taaaaaagag gcagtttgcc
     1381 tcttttttac ataacttaat cataattcca tatcttcatc ctgttcatta tcaacagtga
     1441 tattaaaatc taaattcaaa agctgcttac atagatcagt gtatatgtcc tgctcattta
     1501 aacaggtttc aaacaggtac gtcctgtctc tgtcatattc ttttaatcca cgtaaataat
     1561 acggcttgtc cctatcaaga ataataaaag gaacaatatt attttttaaa cattccttga
     1621 acatgatcgc acgcccaaca cgaccattgc catcaccaaa cggatgaatt ctctcaaagc
     1681 gtaaatggaa gtcaacaata tcttccaatg ttactttgcc tagcctgtta tactcttcaa
     1741 tgagtacgtc tatatctctt tcaacttcgt ctggttttga ggttttaata acatttacca
     1801 gtccaattac attgggatgg attttaaatc ctcctacatt atatctggga tttgattcat
     1861 cagaagttcc cttttttaga attttattca tttcaataat catatcttta tttaacctat
     1921 catcaatatg atccaagatg aaatcaaaga gtttaaaatg atttactgtt tctgtaatat
     1981 catctactct gactaccttc tcgtctttat caggaaaaaa agaattagtt tcaaatatca
     2041 tttcagtctg ttcttctgtt aatcgacttc cttctatttt atttgaatta tatgcaaaca
     2101 atacctgtga aacatgataa atgttaccag gaaattttga ttgtttttgt tctataagtt
     2161 ccttttttaa tttttctata tctgtcattt ccaatctcct atacttatgt tataagtata
     2221 tcactttaaa cataaaatgt aacacaaatt gattattcct ataaaaaaga cgaaatatat
     2281 cttttcctac atctccatgt tatcattgct ttctctatct tctaattctg actgttccct
     2341 taaaattcta ttttcttcat aataaacagt tacacgtaaa aggtcgttta aatccttaat
     2401 tttaagattt ggttttccat catagccgta tattttttat ttctagtgaa actgtattac
     2461 cattatcgtt gaacacccct tcattgtcat accatttctt tattttatta cccgctatca
     2521 caccagcatc atctaaatct ggcataattt ctatttcttc taaatttttt agagcaggat
     2581 tattctttaa attatattta agtgtgttaa gcatcgtacc tactccgctg cacattaata
     2641 catgagcagt tagattattt ctatttagag aaatatagct aagtccatca attacgcttt
     2701 ctgtaactac aatttttttt attccttcaa aattctgata aaatagcccg ttatttttaa
     2761 atgacccttt acagtctata tgaaattttg aattgcccgt acctctgcgg attgcaaaaa
     2821 ctggtttttt ttcttttata ttatttacat catatccaac aaataccaca ttgtgatata
     2881 tatcttcata tacaatgcca tcctctatta gtttgctaac aagtgaatcg cttagttttc
     2941 tttcattggt cagataatga attgctctct cattnnnnnn nnnnatcgtt tttttgtgtt
     3001 aatacaagtc cttggctgat atgactttcg tccatagaaa aatcatacaa atactgctca
     3061 atttcatttg gatttcttga ataaaagtac tctaaaagta tattaattgc atcatatgtt
     3121 gaagtattag tgtattcact gtaaaactca attacattta aaggtactga cccgcaacga
     3181 gcattccagt atactttgtt ttttaaaata tcaaatacca tcgaactatg ttcaactggt
     3241 ttaagatatc gttgacttct actgctttta tgaacagtaa gattaaaaac atttgttgca
     3301 acttctatac atgtaagctc cttgagcatt tcacttacga tagttttacg gtattctttt
     3361 tcagaattgc ttaatatttt tacattagtc atttttattc actcctataa atataaaaag
     3421 aggcaaattg cctcttttta tataattcca ttttgttaca tactttcttt aataacttta
     3481 actatatttt ctataattct ctttcatcta caaattcata ttctgtatat ctaaaatgcg
     3541 tttcgaattc atcacttaaa tatgattttc gaatttcatg attcaatgtt tgaaggtgat
     3601 taggataatg ttcatatata tccagcaaca tttccctatt ttctatgata agtgcctttt
     3661 catctgaatt tattcttagg ttatcaataa aatcatacaa agcgctccat ctatatccat
     3721 gatggaattt ttcagactct tctggatgtt caataataat ccgctctatg ccagttttat
     3781 tattcttttc aaaaaagctt tcgaaacaat actgctcatc tagagtaaca aataagccta
     3841 atatacattt aaaattatca tcatttaaca gtttcttaaa ttcagtacca atattttttt
     3901 cctgcttcaa agacaattta atattattct ttttgagata ctccttaaaa taattattta
     3961 ttgcactatc aaaatcttct tgagatgcaa gctttgtata tttatctttt cccaatttca
     4021 cgtttataaa ttccatattt gctttgtttc tgtaataatc gttgatccag tcgtgacatc
     4081 ctaattgttc catgattttt actggtaaaa caatttgaga attaatatat atttttatag
     4141 ggctaaaaat tcttgacatt aataaattat taggattttc aattgctggc gtaaccgtag
     4201 tcgctccatc tgttaaagcc ctataaatat ggcagtatat atttttttct aataattctt
     4261 tatatgaatc ataataactg gtaaattcgc attcaccata ctcatcataa ttgggtgata
     4321 caatagtatg aagctcaatt ttaaatttct gattgtccat tgtttcttca atccagcact
     4381 ctaagaaaat atcatgcttt ttaaacatac tgctgttatt tctgctgtag aaatcgtttg
     4441 cgattttttt tgcctccttt tcggttaata tgtttgtcat ttttttgaat ttgaacattt
     4501 cttttatttc cttctttctt tgttaaaata taaaaaagag gcaatttgcc tcttttaaga
     4561 tttgctataa cacaattctc ctttattgca taattttaac tatactaatc ttagcatatc
     4621 ctttgtttca aaaaggcaca attttttttc aaaaaagtga acattttttg aataatattc
     4681 actttatttt atttaccttt ttctgttctt tagtaagttt tgtcgtaaca aaacttactc
     4741 tttttaaatt tctagttcga tttcctcagc ttcattttcc tctaatacaa ctccattgtt
     4801 attgtcagtt aaaagattag tgatctcttt ttgtctttgt ctcaaactaa gcagttcttc
     4861 ctgtgattca aattttttat ttataatatc atcaagtttt gcataatttt ctttcatgtt
     4921 attaatttgt ataaaatact tttcataatc tttttttatt tcaaaaattt ttctaatcat
     4981 tctcccaaca ttaactctgc ctactgcgtt taacaatcca gcattgtaca gatagttatt
     5041 cgctccttta aaacagatat tccaaccatt tccaacatta aaaacaagaa aaaattcaaa
     5101 acccatcatt tttccaatag gaattgaatt tttttcttta ttcatgtcag ataatctgat
     5161 gataaaatca ccatactcct ttaaatcggt aaagactttg tctttatatt caactttaaa
     5221 atcaatatcc attttctcat taatcatttc atattcaaaa ttgatatgct ttttaaatac
     5281 agaatagtct ttacttatac gatcgaattt catttctaaa ttttttattt tttcaggcat
     5341 attacgctta tcatcctgtg actttctaat tgcttttctg tgtcttgcca gactggattc
     5401 aagagttttt aagcgtgtgt ctacttcaag tttttctctt attaaaggat taccgcttgc
     5461 aatagcttta atttctgcat acgaaaaagt tgcctttgta tcttcttcaa tttctcgtgc
     5521 acatccactg ccttcaagca gttgattcat atacgttgac ttggtttcta tcagttgcca
     5581 tgagtaggca tcgaatgatt tttttgttgc atatctaaat tcatatactt cattattcat
     5641 atttccctgc ctaaagaccc gtccatttcg ttgtgcaata tctgaggctc tccatggtac
     5701 gtcaacatga tgaattgcaa tcattctttg ctgaacgttt gtacctgcac caagttttgc
     5761 tgttgaacca atcagtactc tgacttctcc tctatttaca tctctataaa gtttttcttt
     5821 ttttagagtt gttttatagt catgtataaa tgcaatttct tcatcattta cacctaattt
     5881 aattaaattg tttttgattt cgtcatatac attgaattgt gatttaggag tagaagaatc
     5941 acaaaaaacg agttgagtag ctttttgatc cctgtactca tcatatattc tatatatctg
     6001 ttctgaaatt acagttaatt tattatctgg agtcaaaccg tagctttcgt caataagacg
     6061 cagatctatt gatgccttct tgctgtcact ggttaccttt aacatattat cttcatatgg
     6121 agcaactgcc gaattatgta ttgcttcagt tctttcgact acatttgcaa tataatcctc
     6181 caactctgga cttggttcta ttgaaactac ctttgcctta tcgcccacat gttttggcgg
     6241 tatagccctt tttctttgca gtttagaatt ttcaagtgca ttattcagca tcgactctgt
     6301 ttgaatttct gcaacctctc taaatgaatt cattaattca ggtatattaa aaaat
//
LOCUS       DAOMTA010000060         6275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_212_length_6275_cov_2.395177, whole genome shotgun sequence.
ACCESSION   DAOMTA010000060 DAOMTA010000000
VERSION     DAOMTA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212_length_6275_cov_2.395177"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLK68_02700"
     CDS             <1..230
                     /locus_tag="PLK68_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89351.1"
                     /translation="DIILLEPQIRYEEVNLERRLKENLTIITVPPISLYASFDGRKVL
                     DYIKRVYNQEIDKKRKKVKESIEKYEINNES"
     gene            208..960
                     /locus_tag="PLK68_02705"
     CDS             208..960
                     /locus_tag="PLK68_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRM89352.1"
                     /translation="MKLIMRADDLGFSEGVNYGIYKSVVDGVITSVGMMPNMASAKHG
                     YELVKDLDIALGQHSNICVGKPLSDPKLIPSLVQKNGNFCSSKEIRARQVDTINVAEC
                     EIELEAQLAKFRAITGRDPDYFEGHAVFSNNFFIALKNVAQRNNLFFENPSLDKEWEK
                     EFGITGLGFMSLDEHGLYDPRTYMEEHLDIIKNNPCAVAIFHPGFLDQYILEHSSFTL
                     IRPMECDFLCSDWLSEWLNKNKIELVNFKNYK"
     gene            complement(1026..1835)
                     /locus_tag="PLK68_02710"
     CDS             complement(1026..1835)
                     /locus_tag="PLK68_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89353.1"
                     /translation="MKEKLLTCGTFAKICGVEKHVLFHYDEINLFKPAYVNDKGYRYY
                     SYRQYDTSKVIIALKKLGMSLKDIQIYLDQRNPQLFLELLNQQEAKLLEYINEMQQIH
                     EMIKNFQTFTTDALNANYDEIKVTYLKETKLLLSANLENTTSKGFADFMNDYTSFIEN
                     NHIITGEFVGVMMNVENIRKNQLSNYSYLFTTTNNLSEQIFVKKGGNYLCGYHHGSYD
                     GLRDSYQRILDYAELHKIKLGKYAFEEYIIFHICEASKDEYLTRITIEIAE"
     gene            1918..2490
                     /locus_tag="PLK68_02715"
     CDS             1918..2490
                     /locus_tag="PLK68_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM89354.1"
                     /translation="MKLILKYLKNYKLLFFFNVISVFGFILVELGIPTIVATMIDVGV
                     TNKDVSFIYMMGGCIALVSLIGVGGTIALGYCCSKISTAITRDIRNDIFAKVQQFTAN
                     EFNQIGTSSMITRTNNDAFQIQQFVNVLLRTALMTPIMFIFSFIMTARASLPLSYIIA
                     ATIPLIILGVVVVAKITKPISENQQSNERI"
     assembly_gap    2562..2571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2572..3762
                     /locus_tag="PLK68_02720"
     CDS             <2572..3762
                     /locus_tag="PLK68_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89355.1"
                     /translation="SENQQSSLDDLNRISRENLSGIRVIRAFNNDQYEQKRFKETNHR
                     FTKYSKKLFKIMTMTQPIFFMLMNVAGLSIYWVAAHLISTGSLEIGQLVAFMDYLFHA
                     MFSIMLFCTVFMMYPRAEVSAKRIEEVFNLDPIIKNKPADSDFDEKVSIEFDHVTFVY
                     PDGEEPVLQDVSFKANKGEMIAFIGSTGSGKSTLVNLVPRFYDVSSGSIKINGKDIRD
                     YDVLELRDKLGVIPQKAVLFSGTIADNIRFGKKDASDEEVEYAAKVAQAYPFIMEKEN
                     GFDEEISEGATNVSGGQKQRLSIARALVRKAQIYIFDDSFSALDFKTDAILRKELKKE
                     MTESIMLVVAQRISSIMEADQIIVLNEGKVVGKGTHHQLLKECQIYHEIATSQLSEEE
                     LANA"
     gene            3755..5533
                     /locus_tag="PLK68_02725"
     CDS             3755..5533
                     /locus_tag="PLK68_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89356.1"
                     /translation="MHKKYSFKKSLKGLIPFIKPYKWQFIIAILMIFAFNISMVLAPT
                     FEGMITTQLASDVAKSSSLTSVDISFGAIIKIVLSLVVIYIIKTVAQMISVVYLTNAI
                     QQAMEDLRNALQNKIRKLPVRYFDNHHFGDVLSRITNDVDAISNALQQSFTQIVSGVL
                     TVTLALTMMYMINPKMAIIGTMIIPLSLLVTKLVVGKSQKLFKNQQDALGELNSTVTE
                     MYTGYNEILLYNQQVESVEKFKKINENLKENAFKAQFVSSTIAPLNALVTYLAIGAVA
                     VVGTADVIAGTFLVGQLQAFIRYIWQINDPLSQISNLSSQIQSAFAALGRVIELLEEP
                     EEVPEANPPKHLSEVAGNVDFEHVKFGYYEENLMKDLNVNVKSGQMVAIVGPTGAGKT
                     TIINLLLRFYDVKGGSIKIDGVDIRDLPREELRSMFGMVLQDTWLYSGTIYDNIRYGR
                     LDARKDEIINAAKMANVHHFIRTLPDGYNSHINEEANNISQGEKQLLTIARAILKDPQ
                     ILILDEATSSVDTRLEKMLQEAMQRVMKGRTSFVIAHRLSTIKSADLILVINNGDIVE
                     QGTHEELLAKQGEYEKLYNSQFAHNS"
     gene            5667..>6275
                     /locus_tag="PLK68_02730"
     CDS             5667..>6275
                     /locus_tag="PLK68_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM89357.1"
                     /translation="MVKILIVEDDNDINNMIHDLLRLNCIESVSAYSGTEALLLLDEH
                     VDLVLLDLMLPGLSGQEVIKEIKKKKDLPVIILTAIDKMDTKLDLFALGADDYLIKPF
                     DNNEPLARVKIQLKHRQVAFSSALAALITYKDIILDENSYKVTCNEIRINLSKIEFKL
                     LKILLESPTRVFTKDILFEMVWDHEDSGDDNTLNVHISKIRSK"
BASE COUNT         2162 a          800 c         1174 g         2129 t
ORIGIN      
        1 atgacattat tttattggag cctcaaattc gttatgaaga agtaaatctt gagcgacgtt
       61 taaaagagaa tctgacaatt ataacagttc cgcctattag tttatatgct tcgtttgacg
      121 gacgaaaagt attggattat atcaaacgag tttataatca ggaaatagat aaaaaaagaa
      181 aaaaagtaaa ggagagtatt gagaaatatg aaattaataa tgagagctga tgacctcggg
      241 tttagcgagg gcgtaaatta tggtatttat aaaagtgtag tagatggtgt aatcactagt
      301 gttggaatga tgcctaatat ggcaagtgct aagcatgggt atgagttggt taaagattta
      361 gatatcgcat taggacagca cagtaatatt tgtgttggaa aaccgttgag tgatcctaaa
      421 ttgattccgt cgttagtcca gaaaaatggt aatttttgtt ccagtaaaga gattcgggct
      481 cgtcaagtag atacgatcaa tgttgctgaa tgtgaaattg agctggaagc tcaattagcg
      541 aagtttcgag caatcacagg tagagaccca gattattttg aaggtcatgc agtattttct
      601 aataatttct ttattgcttt aaaaaatgtt gctcaaagaa ataatttatt ctttgaaaat
      661 cctagcttgg ataaagagtg ggaaaaagaa tttggaatta ctggattagg atttatgagc
      721 ctagatgaac atggattgta tgatcctcgt acatatatgg aagagcattt agatattatt
      781 aaaaataatc cttgtgcagt agctattttc catcctgggt ttttagatca gtatattttg
      841 gaacattcgt cttttactct tattagacct atggaatgtg attttctttg ttccgattgg
      901 ttaagtgaat ggttaaataa aaataagata gaattagtaa attttaaaaa ttataagtaa
      961 aagtaggtaa tttaagatct tcctgcccat tggttattta acttgggagt cttataccta
     1021 cttttttatt ctgctatttc aattgtaata cgagttaagt attcatcttt ggaagcttca
     1081 caaatatgaa aaataatata ttcttcaaaa gcatatttac ctaatttaat tttatgaagc
     1141 tctgcataat cgaggattct ttgatatgag tcacggagac catcataact tccatgatga
     1201 tagccacata aataattacc acccttttta acaaatattt gttcactcag attattagtt
     1261 gttgtaaata aataagagta gttagataat tgatttttac gaatattttc gacattcatc
     1321 attaccccaa cgaattcgcc agtaataata tgattatttt caataaaaga tgtgtaatca
     1381 ttcataaagt cagcgaagcc tttagaggta gtgttttcga gattagcaga taataataat
     1441 ttagtttctt ttaaataagt tactttgatt tcatcataat tagcatttaa agcatcagtt
     1501 gtaaatgttt gaaaattttt gatcatttcg tgaatctgct gcatttcatt aatatactca
     1561 agcaatttag cttcttgttg attcaataac tctaaaaata attggggatt gcgctgatca
     1621 agatatattt gaatatcttt taacgacatt ccgagttttt ttaaagcaat aattacttta
     1681 gatgtatcat attgacgata cgagtaatag cggtaacctt tatcattcac atatgctggt
     1741 ttaaataaat taatctcgtc ataatgaaaa agaacatgtt tttcaacacc gcatatttta
     1801 gcaaaagttc cacatgttaa taatttttct ttcatttaaa taactccttg actataaggt
     1861 taccttatag tttatcatat gatatgttgc ttcacaatac aatgaaagga gaaaaatatg
     1921 aaattaatct taaagtattt aaaaaactac aaattactat tcttttttaa tgtaatttca
     1981 gtttttggat ttattttagt tgaattagga attccgacca ttgtagcaac aatgatagat
     2041 gttggggtta ctaataaaga cgttagcttc atctatatga tgggtggctg tattgcctta
     2101 gtttcactta tcggtgtggg tggaaccatt gccctaggat attgttgttc aaaaatatca
     2161 actgcaatta ctcgtgatat tagaaatgat atctttgcta aagtacaaca atttacagct
     2221 aatgaattta atcagattgg gacatcatca atgattacaa ggacgaataa tgacgctttt
     2281 caaattcaac aatttgttaa cgtattgctt cggacagctt tgatgacacc aattatgttt
     2341 atttttagtt ttattatgac agcgcgggct tcgttaccgt tatcatatat tattgctgct
     2401 acaattccgt tgattatcct tggggttgtc gtagtagcga agattactaa accaatttca
     2461 gaaaatcaac aaagtaatga aagaatataa gttcaaaatc aacggcaagg attttcatgt
     2521 tgccgtaaat gggataagtg gcacaaaggc tgatgtcact gnnnnnnnnn ntcagaaaat
     2581 caacaaagtt cattagatga cttgaatcgt atttcgagag aaaatcttag cgggattcga
     2641 gtaattagag cttttaataa tgatcaatat gaacaaaaac ggtttaaaga aacaaatcat
     2701 cgctttacta aatattcaaa aaaattattt aaaattatga ccatgacaca gccaattttc
     2761 tttatgctga tgaatgttgc tggtttgtca atatattggg tagcagctca tctaatttct
     2821 actggttctt tagaaattgg acagcttgtt gctttcatgg attatttatt ccatgcgatg
     2881 ttttcgatta tgcttttctg tacggtgttt atgatgtatc ctcgggctga agtgtcagcg
     2941 aaacgaattg aagaagtctt caatttagat ccaataatta aaaataaacc tgcagatagc
     3001 gattttgatg aaaaagtaag tattgagttt gatcatgtaa cttttgttta tcctgatggt
     3061 gaggagccgg ttcttcagga tgtttctttt aaagcgaaca aaggcgaaat gattgctttt
     3121 attggaagta ctggttctgg taaaagtaca ttagtgaatt tagtaccaag attttatgat
     3181 gttagtagtg gaagtattaa aatcaatggg aaagatattc gtgattatga tgtattagaa
     3241 ttacgagata aattaggtgt gattcctcaa aaagcagtat tatttagtgg gacaattgct
     3301 gataatattc gttttgggaa aaaggatgct agtgatgaag aagtagaata tgctgctaaa
     3361 gttgcgcagg cttatccatt tataatggaa aaagaaaatg gctttgatga agaaatcagt
     3421 gaaggagcga caaatgtttc gggtggtcaa aaacaacgat taagtattgc tcgggcatta
     3481 gttcgtaaag ctcaaattta tatctttgat gattcttttt cagcattgga ttttaaaaca
     3541 gatgcaattt taaggaaaga gttaaaaaaa gaaatgaccg aatcaatcat gctagttgta
     3601 gctcaaagaa tttctagtat tatggaggca gatcagatca ttgttttaaa tgaaggtaag
     3661 gtcgtaggaa aaggaacaca tcatcaatta ttaaaggaat gtcaaattta tcatgagatt
     3721 gcaacttctc aattaagtga ggaggaatta gcaaatgcat aaaaaatata gttttaaaaa
     3781 aagtttaaag ggtttaatac catttattaa gccgtataaa tggcaattta ttattgctat
     3841 cctaatgatt tttgctttca atatttcaat ggttttagca ccaacatttg aaggaatgat
     3901 cactactcaa ttagcgagcg atgttgctaa aagcagtagt ttaacaagtg tggatattag
     3961 ctttggggca attattaaaa ttgtacttag tttagtagtc atttatatta ttaaaacagt
     4021 tgctcaaatg atttcagtag tttatttgac gaatgcaatt cagcaagcaa tggaagatct
     4081 tcgtaatgct ttacaaaata agattcgtaa attaccagta agatattttg ataatcatca
     4141 ttttggagat gttttaagtc gaattactaa tgatgttgat gctatttcta acgctctaca
     4201 acaaagtttt acgcagatcg tcagtggagt attaactgtt actttagcgt taacaatgat
     4261 gtatatgatt aatcctaaga tggcaattat tggaacgatg attattccat tatcgctatt
     4321 agtaactaag ctagttgtag gaaaaagtca aaaattattt aaaaatcaac aagatgcgct
     4381 tggtgaatta aatagtactg taactgaaat gtatacagga tataatgaaa ttttattata
     4441 taatcaacaa gtggaatcag ttgaaaaatt taaaaaaatt aatgaaaatt taaaagaaaa
     4501 tgcctttaaa gcacaattcg tttcgtcaac gattgcacct ttaaatgcat tagttactta
     4561 tttagcaatt ggggcagttg ctgtagtggg aactgctgat gtaattgcag gaacattttt
     4621 agttgggcag ttacaagctt ttattcgtta tatttggcaa attaatgatc ctttatcgca
     4681 aatatctaac ttatcatcac aaattcagtc tgcttttgca gcactaggaa gggtgattga
     4741 gttattggaa gaaccggaag aagtaccgga agctaatccg cctaaacatt tgagcgaagt
     4801 ggctggaaat gttgattttg aacatgttaa gtttggttat tatgaagaaa acttaatgaa
     4861 agatttgaat gttaatgtta aaagtggtca gatggttgca attgttggac cgactggagc
     4921 aggaaaaaca acaattatta atttactatt acgtttttac gatgttaaag gtggtagtat
     4981 taaaatagac ggcgttgata ttcgtgattt accacgggaa gagttacgtt ctatgtttgg
     5041 aatggtttta caggatacat ggctatattc tggaacaatt tatgataaca ttcgttatgg
     5101 tcgtttggat gctcgcaaag atgagattat taatgctgct aaaatggcca atgttcatca
     5161 ttttattaga actctgccag atggatataa ttcacatata aatgaagaag ccaataacat
     5221 ctcgcaaggg gagaaacaat tattaacgat tgcccgagca atcttaaaag atcctcaaat
     5281 cttgattctt gatgaggcga cttcatcggt tgatacccgt ttagaaaaaa tgctgcaaga
     5341 agcaatgcag cgggttatga aagggcggac aagttttgtt attgcccatc gtctatcaac
     5401 tattaagagt gctgacttga ttttagtaat caataatggt gatatcgtcg aacaaggaac
     5461 acacgaagaa ttattagcaa aacaaggtga gtatgaaaaa ttgtataatt cacaatttgc
     5521 tcataattca taatccagaa gtatctaaac ttagatactt ctttttctta ttagaaatac
     5581 aaagataagg tcttaaaacc aatatgtctt ggagaggtat attgtgttat actatcactg
     5641 tgtaagtgaa ggaatgtagt ataacaatgg taaaaatatt gatcgtagaa gatgacaatg
     5701 atattaataa tatgattcat gatctattaa gattaaattg tatcgaaagt gttagtgctt
     5761 attctgggac agaggcatta cttttattag atgaacatgt agatttagta cttttggatt
     5821 tgatgttacc gggtcttagt ggtcaggaag taattaagga aatcaaaaag aagaaagatt
     5881 taccggtgat tattttaact gcaatcgata aaatggatac aaaacttgac ctatttgctt
     5941 tgggagctga tgactatttg atcaaaccat ttgataataa tgaaccttta gcaagagtaa
     6001 agatacaatt aaagcatcgg caagtagctt tttcatcagc tctagcagct ctaattactt
     6061 ataaagatat tattcttgat gaaaatagtt ataaagtgac ttgtaatgag ataagaataa
     6121 atttatctaa aattgagttt aaactattaa aaatattatt agaaagtcca actcgtgttt
     6181 ttacaaaaga tattttattt gaaatggttt gggaccacga agattcaggt gatgataata
     6241 cgctaaatgt tcatattagt aagattcgta gtaag
//
LOCUS       DAOMTA010000061         6224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_216_length_6224_cov_2.743881, whole genome shotgun sequence.
ACCESSION   DAOMTA010000061 DAOMTA010000000
VERSION     DAOMTA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216_length_6224_cov_2.743881"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLK68_02735"
     CDS             <1..600
                     /locus_tag="PLK68_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRM89358.1"
                     /translation="FVYAGKKVALADPTVKPKGIVLVVETGVKAIDDYMKSIMPKKFA
                     KNYYPYFAMVFCYILVSNLSSLIGFESPTSNYSITFAMTFITFILIQYNALKKKGFFK
                     YVWDTVWPPTNILSVLSPLISISMRLFGNILSGSILMTLIYQFTAWISFHVINFNFLG
                     PIIAPVFHCYFDIFAGCIQTLVFVTLSSILIMMENEDDE"
     gene            675..902
                     /gene="atpE"
                     /locus_tag="PLK68_02740"
     CDS             675..902
                     /gene="atpE"
                     /locus_tag="PLK68_02740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRM89359.1"
                     /translation="MTDVGLIAIGAGIAVCAGLGTGIGEGICASKAVEALGRNPEMEG
                     KIRTLMILGIALTETAAIYGLLISLILLFVY"
     gene            925..1425
                     /gene="atpF"
                     /locus_tag="PLK68_02745"
     CDS             925..1425
                     /gene="atpF"
                     /locus_tag="PLK68_02745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRM89360.1"
                     /translation="MPDIASKLFPNVTTIIIQLLSTGVLLLVFKKYLWVPVQNYFAKR
                     AEFIEGTVNEAKDMNEKARALMEESEEQARQAAVQYREIVNLAKEDALKTKATIQEQA
                     NQEYKAKLDQARREIEAEKAQAKAAMKQEIVEVAIDVATKVMNKEMDTKTNKALVEDF
                     VEEVVN"
     gene            1425..1958
                     /locus_tag="PLK68_02750"
     CDS             1425..1958
                     /locus_tag="PLK68_02750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRM89361.1"
                     /translation="MDAVAVRYAESLFDLAKEMNQVEAYSKDIDLIRTVFESDPSFVP
                     FFSHVLIEDEAKCALLDKSFKGQVNDYVVNFLKLLVRKRRMRYVMEIIEAFKALTNEH
                     FGILEGILYANYDISVQEVKEVEDALSKKENKTIRLRVVNDPSLIGGIKVEINNRVFD
                     GSIKNKVALLKKELLRK"
     gene            1968..3491
                     /gene="atpA"
                     /locus_tag="PLK68_02755"
     CDS             1968..3491
                     /gene="atpA"
                     /locus_tag="PLK68_02755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRM89362.1"
                     /translation="MGLRPDEISALIKEQIKHFDDVIELKDVGTVMTVGDGVSLIHGL
                     DNAMLGELLAFPNDVYGMVMNLDEDCVGAVLLGSESTIKEGDEVKRTGKIMEIPVGDE
                     MLGRVVNPLGQAIDGNGEIITAHTRPIERVASGVMTRKSVDQPLQTGITSIDAIIPIG
                     RGQRELIIGDRQTGKTAIAIDTILNQKDQDIYCVYVAIGQKNSTVAQIVEKLRKGGAM
                     EYTTVVSASASELAPVQYIAPYAGCAIAEEWLDQGKDVLIVYDDLSKHAVAYRTVSLL
                     LKRPPGREAYPGDVFYLHSRLLERACRLNEENGGGSITALPIIETQAGDISAYIPTNV
                     ISITDGQIFLQQELFNAGFRPAIDTGLSVSRVGSAAQIKAMKQVSGSLKLELAQYAEM
                     QAFAQFGSDLDAATKATLDHGAKVREVLKQAQYSPRTVSTQVITLFALKYGFTKTLEV
                     EQVSAFMDQLVEHINMTQRDIIDEINEQKAISADLEKRMKDVMSAFVTQFEKTQTKG"
     gene            3498..4355
                     /gene="atpG"
                     /locus_tag="PLK68_02760"
     CDS             3498..4355
                     /gene="atpG"
                     /locus_tag="PLK68_02760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRM89363.1"
                     /translation="MPGGMQEIKRRIKSVESTKKITKAMELVATSKLRKTRNQLEQSK
                     PYYTNVAQTVAEILANCKGNNDSIYLVENKDIEKEVFIVIASSLGLCGGYNANIFKEI
                     KGAIKPGDYVYSIGSKATSYLLKNHQGVTDHKFDDLNTTFDFKDVTKLVAELTKMYRE
                     KEISKIKIVYTEFVNNLTFRPRIVTLLPVDPSDFDHIEISKKSTLFEPSPEEVLDSLI
                     PMYLQAVIYGYIIESATSENAARRTSMENANDNADELTEQLLLKYNQARQTAITNEIS
                     EIVAGANAQ"
     gene            4367..>5733
                     /gene="atpD"
                     /locus_tag="PLK68_02765"
     CDS             4367..>5733
                     /gene="atpD"
                     /locus_tag="PLK68_02765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRM89364.1"
                     /translation="MSQNIGKIISAKGPVIDIQFDGDNNLPALNTAIEIQNGDELLVV
                     EVAQHIGDDVVRCVAMGPTDGVKRGMDALNTGNPISVPVGNETLGRMFNVLGQPIDGK
                     EALGDSTKKMPIHRSAPTYAEQRTETEILETGIKVVDLICPFIKGGKIGLFGGAGVGK
                     TVLIQEFINNIATEHGGLSVFAGVGERSREGNDLYYEMKESGVLSKTTLVFGQMNEPP
                     GARLRVALTGLTMAEEFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRVPSQAGYQPT
                     LATEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFAHLDAKVVLDRSIAAL
                     GIYPAVDPLNSSSRALDPLVVGTEHYEVAHGVQQILQRFQELQDIIAILGMDELGEED
                     KLTVARARRVRNYLSQPFTVASQFTGMDGKYVRVADTIKGFKEILEGKHDDLPEQAFH
                     NVGTI"
     assembly_gap    5734..5743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5852..>6224
                     /gene="atpC"
                     /locus_tag="PLK68_02770"
     CDS             5852..>6224
                     /gene="atpC"
                     /locus_tag="PLK68_02770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRM89365.1"
                     /translation="MTKFKLKIVTPSGIYQEVEVNQLNLRTTAGQVGILAHHMPLASG
                     IEISEMSYIIDKQRHIFAIGGGFIYVNDDETKIIANSIESKEEIDLNRAKEAKRRAEQ
                     RIKEVTEKTDLLRAEIALKKAI"
BASE COUNT         2200 a          880 c         1271 g         1863 t
ORIGIN      
        1 tttgtctatg ctggaaaaaa agtagcattg gcggatccaa ctgtcaaacc aaaaggaatt
       61 gtattggttg ttgaaacggg agttaaagct attgatgatt atatgaaatc aatcatgcct
      121 aagaaatttg ccaagaacta ttatccatat tttgcaatgg tattttgtta catattggtc
      181 agtaacttat caagtttgat tgggtttgaa tcaccaacct caaactactc tattaccttt
      241 gcgatgacat ttattacttt tatattgatt caatataatg cgttgaagaa aaagggattt
      301 tttaaatatg tgtgggatac tgtgtggccc ccaactaata ttttaagtgt cttatcacca
      361 ttaatttcaa tttcaatgcg tttatttgga aatattttga gtggatctat tttaatgacg
      421 ttgatttatc aatttacagc atggatttca ttccatgtaa tcaactttaa ctttttaggt
      481 ccaattattg cccctgtatt ccattgttat tttgatattt ttgcagggtg tatccaaaca
      541 ttagttttcg taactttatc atcaatctta attatgatgg aaaacgaaga cgacgagtaa
      601 gctctaatct aggtatccta gttagatata aattacaaaa aaatatatag aaaatattgg
      661 aggaaattta aattatgaca gatgtaggtt taattgcaat tggtgcaggt attgcagtat
      721 gtgctggttt aggaacaggt atcggagaag gaatttgtgc gagtaaggcg gtagaagctt
      781 taggtagaaa tcctgaaatg gaaggtaaaa ttagaacgtt aatgattcta gggattgcat
      841 taactgaaac tgcagctatc tatggtttat taatttcatt aattttatta tttgtatact
      901 aatattagct aaggaggaat acgtatgcca gatatagcat cgaaattatt ccctaatgta
      961 actacaatta ttattcaatt actttcaaca ggagttttac ttcttgtctt taaaaagtat
     1021 ttatgggttc ccgttcaaaa ttattttgct aaacgggcag aatttattga aggaactgta
     1081 aacgaagcta aagatatgaa tgaaaaagct agagcactga tggaagaaag tgaagaacaa
     1141 gcacgccaag cggcagttca atatcgtgaa attgttaatt tagctaaaga agatgcttta
     1201 aaaacaaagg caacgattca agaacaagct aatcaggaat ataaagctaa actagatcaa
     1261 gcacgtcgtg agattgaggc tgaaaaagcg caggcaaaag cagcgatgaa acaagaaatt
     1321 gttgaggttg cgattgatgt agcgactaaa gttatgaaca aagaaatgga tacaaagact
     1381 aacaaagctt tggttgaaga ctttgttgaa gaagtggtta actaatggat gcagtagcag
     1441 taagatatgc agaatcacta tttgatttag ctaaagagat gaatcaagtt gaagcatatt
     1501 ctaaagatat tgatttaatt agaactgttt ttgaaagtga tccaagtttt gttccgttct
     1561 ttagtcatgt tttaatagaa gatgaagcta aatgtgcatt attagataag agttttaaag
     1621 gacaggttaa tgattatgtc gttaattttc taaaactttt agttagaaaa agaagaatgc
     1681 gttacgttat ggaaataatt gaagcattta aagcgttaac gaatgagcat tttggaatct
     1741 tagaaggtat tttatatgct aactatgata tcagtgtgca agaagttaaa gaagtggaag
     1801 atgctttaag taaaaaggaa aataaaacga ttcgtttaag agtagttaat gatccatcac
     1861 tgattggtgg aattaaagtc gaaataaata atcgtgtttt tgatggctct atcaaaaaca
     1921 aagttgcatt attgaaaaaa gaactattaa gaaagtaggt gaaaatagtg gggcttagac
     1981 cagatgaaat tagtgcttta attaaagaac aaattaagca ctttgatgat gtaatcgaac
     2041 ttaaagacgt tggaacagtc atgactgttg gcgatggtgt tagtttaatt catggtttag
     2101 ataatgccat gctaggtgaa ttacttgcct tccctaatga tgtttatgga atggtaatga
     2161 acttagatga agattgcgtc ggagccgtac tacttggatc tgaaagtacg attaaagaag
     2221 gagacgaagt taaacgtact ggaaagatta tggaaatacc agttggggat gagatgttag
     2281 gtcgagttgt aaatccttta ggacaagcaa ttgatggtaa tggagaaatc attacagcac
     2341 atactcgccc aattgaaaga gttgccagcg gggttatgac aagaaagtca gttgaccaac
     2401 cgttacaaac aggaattact tcgattgatg caattattcc aatcggtcgg ggtcaacgtg
     2461 agttgattat tggagatcgt caaacaggaa agactgcgat tgcaatcgat actattttaa
     2521 atcaaaaaga tcaagatatt tattgtgttt acgtagcaat tggacaaaag aattcaactg
     2581 ttgcccaaat tgttgaaaag ttacgtaaag gtggcgcaat ggaatataca acagttgtgt
     2641 cagcatctgc tagtgaactt gcaccagtgc aatatattgc accgtatgca gggtgtgcaa
     2701 ttgctgaaga atggttagat caaggtaaag atgttttaat tgtttatgat gatctatcta
     2761 agcatgccgt tgcttatcgt acggtatcat tattattaaa aagaccacca gggcgtgaag
     2821 cttatcctgg agatgtcttc tatttacatt cgagattatt agaaagagca tgccgcttaa
     2881 atgaagagaa tggtgggggt tcaatcacag ccttaccaat tattgaaaca caagcaggtg
     2941 atatctcagc atatattcca actaacgtaa tttcaattac agatggacaa attttcttac
     3001 aacaagaatt attcaatgct ggatttagac cagcaattga tactggactt tcggtaagtc
     3061 gggttggttc agctgcacaa atcaaagcaa tgaagcaagt atcaggttca ctaaaacttg
     3121 aattagcgca gtatgctgaa atgcaagcat ttgctcaatt tggttcagac ttagatgctg
     3181 ctacgaaagc aactttagat catggtgcaa aagtgcgtga ggttttaaaa caagcacaat
     3241 attcaccacg aactgtatca acgcaagtaa ttacattatt tgctttaaaa tatggattta
     3301 ctaagacatt agaagtggaa caggtgtctg cttttatgga tcagttagtt gaacatatca
     3361 atatgacgca aagagatatt attgatgaaa tcaatgaaca aaaagcaatt tctgctgatt
     3421 tagaaaaacg catgaaagat gttatgagtg cttttgtaac acaattcgaa aaaactcaaa
     3481 caaaggggta gttaactatg cctggaggaa tgcaagaaat taagcggaga attaaatcag
     3541 ttgaatcgac taaaaaaatt actaaagcaa tggaattagt cgcaacttct aaacttcgta
     3601 aaacaagaaa tcaattagaa cagtcgaaac cttattatac taatgttgct caaactgtag
     3661 cggagatctt agctaattgt aagggtaata atgatagtat ctatttagtt gaaaataaag
     3721 atattgaaaa agaagtattt attgttattg cttcaagtct aggactatgt ggtggatata
     3781 atgccaatat ctttaaagaa attaaaggtg caatcaaacc aggtgattat gtttatagta
     3841 ttggttcaaa ggcaactagt tatttattaa aaaatcatca aggggtaacg gatcataagt
     3901 ttgatgattt aaatacaact tttgatttta aagatgtaac taagttagtt gctgaattaa
     3961 ctaaaatgta tcgtgaaaaa gaaattagta aaattaagat tgtttatacg gagtttgtta
     4021 ataacttaac atttagacca agaattgtta cgttattacc tgttgatcca agtgattttg
     4081 atcatattga aatctcaaag aaatcaacat tatttgaacc aagtccagag gaagtattag
     4141 acagtttgat tccaatgtat cttcaagcag tgatttatgg ttatatcatt gaatcagcta
     4201 caagtgaaaa tgccgcacga agaacatcaa tggaaaatgc taatgataat gccgatgaac
     4261 ttacagaaca attattatta aaatacaatc aagctcgtca aactgcgatt actaatgaaa
     4321 ttagtgaaat cgtagcagga gctaatgcac aataaggagg tgccgtatgt cacaaaatat
     4381 tggtaaaata attagtgcca aagggccagt aattgatatc caatttgatg gtgataacaa
     4441 tttaccagca ttaaatacgg cgattgaaat ccaaaatggt gatgaacttc tagtagttga
     4501 agtcgctcaa catatcggag atgatgtagt tcgttgtgtt gcaatgggac caactgatgg
     4561 ggtaaaacga ggaatggatg ctcttaatac gggaaatcca atttctgtac ctgtcggaaa
     4621 tgaaactcta ggaagaatgt tcaacgtttt aggacaacca atcgatggta aagaagcatt
     4681 aggtgatagc actaagaaaa tgccaattca ccgatctgca ccgacatatg ccgaacaaag
     4741 aacagaaact gaaattcttg aaacgggaat taaagtagtt gatttgatct gcccatttat
     4801 caaaggtgga aagattggat tattcggtgg tgccggagta ggtaaaacag tattgattca
     4861 agaattcatt aataacattg ctacagaaca tggtggttta tcagtttttg ctggagttgg
     4921 tgaacgtagc cgtgaaggta atgatttata ttatgaaatg aaggaaagtg gtgttttatc
     4981 taaaacaaca ctagtatttg gacagatgaa tgaaccccca ggagctcgtt taagagttgc
     5041 tttaacgggt cttactatgg cagaagaatt ccgtgatgaa caaggtcagg atgtcttatt
     5101 attcatcgat aatattttcc gttttactca agctggatct gaagtatctg ccttacttgg
     5161 acgggtacca tcacaagctg ggtatcagcc aactttagca accgaaatgg gcgctttaca
     5221 agaacggatt acatcaacta aaaaaggatc tattacttca gttcaagcgg tatatgtacc
     5281 tgctgatgac ttaactgatc ctgctccagc aacaacattt gctcatcttg atgctaaagt
     5341 agtattagat cgttcaattg cggctttagg aatttatcct gccgtagatc cattaaattc
     5401 ttcatcaaga gcccttgatc cattagttgt agggactgaa cactatgagg tagcccatgg
     5461 ggttcaacaa atcttacaac ggttccaaga attacaagat attattgcta ttttaggaat
     5521 ggatgagtta ggtgaagaag ataaattaac agtagctcgg gctcgtcgtg tccgtaacta
     5581 cttatcacaa ccatttacag tagctagtca gtttactggt atggatggta aatatgtccg
     5641 tgttgctgat acaatcaaag gatttaaaga aatcctggaa ggtaagcatg atgatttacc
     5701 agagcaagca ttccacaatg taggtacaat tgannnnnnn nnnttttaat aaaataacac
     5761 aaaataacga cgacaacaac taataccatg tcaaaaaaca tacaactaaa cacgattgaa
     5821 gaagctattg aaaaagcgaa gacattagca aatgactaaa tttaagctta agatagtaac
     5881 accaagtggt atctaccaag aagtggaagt taatcagtta aatcttcgca caacagcagg
     5941 acaggtagga atcttggctc atcatatgcc tttagcaagc gggattgaaa tctctgagat
     6001 gagttatatc attgataaac aacgtcatat atttgccatc ggtggtggat ttatatatgt
     6061 taatgatgat gaaacaaaaa taattgctaa ttcaattgaa tctaaagagg aaattgattt
     6121 gaaccgtgct aaagaagcta aacgtcgggc ggaacaaaga attaaggaag taaccgaaaa
     6181 aactgactta ttgcgggctg aaattgcact taaaaaggca atta
//
LOCUS       DAOMTA010000062         6210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_217_length_6210_cov_2.255240, whole genome shotgun sequence.
ACCESSION   DAOMTA010000062 DAOMTA010000000
VERSION     DAOMTA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217_length_6210_cov_2.255240"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLK68_02775"
     CDS             <1..508
                     /locus_tag="PLK68_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM89366.1"
                     /translation="GKELIPISVNISRLTLLESDFTNSLKRIKNKYNIPNNFITLEIT
                     ESIGNIDRSIIATISKRIKDLGFNISLDDFGAKYANMSLLSTLNFDELKIDKSMIDTL
                     VNNDKCQTILHHIIEMCKKINVACVAEGVETEKQIELLVCLGCNIIQGFYYSKPIPLQ
                     EFEDKYHQ"
     gene            654..2075
                     /locus_tag="PLK68_02780"
     CDS             654..2075
                     /locus_tag="PLK68_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRM89367.1"
                     /translation="MISIQELLGLINEFLYSNILIALLVATGVYFTIRTKFVQFRMLP
                     EGIRLLKEKSHHDDGVSSFQALMISTASRVGTGNIAGVATALAAGGAGSIFWMWIIAL
                     IGGASAFIESTLAQVYKEKDGDSFRGGPAYYIEKAIGKRWLGIIFSCLLIACFIFGFN
                     PLQAYNVSSAVEYYFSNNELVAFVIGAVLALATAAVIFGGVHRIGIISSKVVPVMAIL
                     YILLGLYITFSNLNQLPEIFSDIFNQAFDFKAIAGGFAGSCVMHGIKRGLFSNEAGMG
                     SAPNAGATADVSHPVKQGLVQTISVFIDTMLICSTTAFMLLNYGTESGLTGMPYVQQA
                     IFAEVGEFGIHFITISIFLFAFSSLIGNYCYAESNFKFIIDNKKALFIFRIITVIIIF
                     FGAQASFNTIWDLADVLMGFMAIMNIVVILLLGKIAFKCLKDYSIQKKEGKDPIFHPD
                     NLGIKNAEFWHDIEKEYEKPVEV"
     gene            complement(2089..2361)
                     /locus_tag="PLK68_02785"
     CDS             complement(2089..2361)
                     /locus_tag="PLK68_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89368.1"
                     /translation="MALFDDLSKKAAKLTEKTIEKSSELADVAKTRVSIKSAQADLDE
                     KFIELVKLYYEIISNDNIFDEKTAAIVQEIDSINTRIKELEANLDK"
     gene            2568..2990
                     /locus_tag="PLK68_02790"
     CDS             2568..2990
                     /locus_tag="PLK68_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRM89369.1"
                     /translation="MPLFVGTLSAFLTHDAMTTFETIQKPVLSPPGWLFPIVWTVLYI
                     LMGIASYLVLTTESSQHVTLFIYKIQLFFNFIWPIIFFNLRLYLLAFIWLIILWLLIL
                     ITAILFYKTNKLAGYLMLPYLIWVTFAGYLNLSSYLLN"
     gene            complement(3041..3475)
                     /locus_tag="PLK68_02795"
     CDS             complement(3041..3475)
                     /locus_tag="PLK68_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89370.1"
                     /translation="MIEPDTIKLLRECDAGIKMGAASIDDVLDYAHDETLRQHLIDCK
                     REHEQLKEEIQTLLDKFHDEGKEPNPMAKGMSWVKTNAKLVMHESDQTIADLITDGCN
                     MGVKSLNKYLNQYEAADEKTKDITKRLINLEEKLVIDIRQFL"
     gene            complement(3539..4489)
                     /locus_tag="PLK68_02800"
     CDS             complement(3539..4489)
                     /locus_tag="PLK68_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRM89371.1"
                     /translation="MKVVVIGACGHIGSYLVPKLVKGGFKVVAISRGKSKPYINDPAW
                     KMVEQVVLDRNKDEDFAYKVAKMNADIVVDLINFNIEDTKKMVTALKEIKISHYLYCS
                     SIWAHGRAEFLPADPNSLKEPLDDYGVDKYQSELYLKEQYRKNGFPATIIMPGQISGP
                     GWTIINPLGNTDFSVFQKIANGQEIYLPNLGMETLHHVHGDDVAQMFYQAITHRNQAL
                     GESFHAVERESMTLYGYAKAMYRYFNQEPKIKFLSWEKWCKYVNDDELIDHTYYHIAR
                     SGEYSIENAQKLLEYSPKYTTLETVEQAVASYIERGIITL"
     gene            4700..5593
                     /locus_tag="PLK68_02805"
     CDS             4700..5593
                     /locus_tag="PLK68_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89372.1"
                     /translation="MLLQEKMKNTKFSPNEEIVIDFILTKQERIKDYSTTMIAGETYT
                     SPSLLVRISKKLGFSGYNNFKNAFLEEVQYLHKSHLNIDANQPFLKTDSIMNIANKIT
                     QLKTESLNDTLSLIHHDTLQKAVRALQKSNTVKVFGVSNLSFPAEEFVFKLRHIGKNA
                     EVFVLNSNIYQEAMMANSNDLGICLSYSGESGEIIKIANILKKKNVPIIAITSIGENS
                     LTRLADIVLRVTTREKSYSKIGAYSSLESISLILDVLYSCFFTTAYDQHYTFKTELAK
                     NTESREITNLIIQENEQNEKE"
     gene            complement(5620..>6210)
                     /locus_tag="PLK68_02810"
     CDS             complement(5620..>6210)
                     /locus_tag="PLK68_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89373.1"
                     /translation="EVGLRDFAELKELLLSTDLSFEDHSHATSKQARLHDYSHKVRES
                     IIMVEKSIDMDAVIDLCKDINEYQKVAIFGLLKAGAVAFNLQGDLLMLNKQVYTNISY
                     SQQIQYIVAADEDDLIIIFSYTGAYFDYQDLRALKKRLTAPKIWMISSDDREYPECID
                     RTILFKSLQDQNSHPYQLQFIAGLIAQEYSRLHQLK"
BASE COUNT         2002 a         1037 c          866 g         2305 t
ORIGIN      
        1 aggaaaagaa ttaattccta tttctgtcaa tatttctcgc cttacccttc tagaatctga
       61 ctttactaat tccttaaaac gaattaaaaa taagtataat attcctaaca atttcataac
      121 tcttgaaatt actgaaagca ttggaaatat cgatcgtagt atcattgcta cgatttctaa
      181 gcgcattaaa gatctcggtt ttaatatttc actagatgac ttcggtgcta aatatgcaaa
      241 tatgtcatta ttatcaactt taaattttga tgaattaaaa attgataaat caatgatcga
      301 cacattagta aataacgata aatgtcaaac tatcttacat catataattg aaatgtgtaa
      361 aaaaattaat gttgcctgtg tcgctgaagg agttgaaact gaaaaacaaa tagaactgtt
      421 ggtttgttta ggctgcaata ttattcaagg gttttattat tctaaaccaa ttcctttaca
      481 agaatttgag gataagtatc atcaataatt tgtctaaaag tgtatcctac tggatacact
      541 ttttgtgcat tcttatttac agtagaaatc aaacatggta acatacaaaa gtatgattat
      601 aatgcatttt tgcgctttat aatcaagatt aattaatgaa aggaagtgaa tgcgtgatat
      661 caattcaaga attacttgga ttaatcaatg aatttttata tagcaatatt ttaattgctc
      721 tgcttgttgc aaccggggtg tattttacaa ttcgaactaa atttgttcaa tttagaatgt
      781 taccagaagg aattcgactt cttaaggaaa aatcacatca tgatgatggt gtttcatctt
      841 ttcaagcact aatgatttca actgcatcac gagtaggaac tggtaatatt gctggtgttg
      901 ctacagcttt ggctgctggt ggagctggtt ctattttttg gatgtggata attgctctga
      961 ttggtggagc cagtgctttt attgaatcaa ccttagccca agtctataaa gaaaaagacg
     1021 gcgactcatt tcgtggagga ccagcttatt atattgaaaa agctatcgga aaacgctggt
     1081 taggaattat tttttcgtgt ctgttgattg cttgctttat ttttggcttt aatccattgc
     1141 aagcatataa tgttagtagt gccgtcgaat attatttttc aaacaatgaa ttagttgcct
     1201 ttgttattgg tgcagtttta gctttagcta ctgctgcggt tatttttggc ggagtccatc
     1261 gaatcgggat cattagctcc aaagttgtac cagtcatggc tattttatat atattacttg
     1321 gtttatacat aacctttagc aatcttaatc agctaccaga aatatttagt gatattttta
     1381 atcaagcatt tgattttaaa gcgattgcag gaggatttgc cggctcttgt gtaatgcatg
     1441 gaatcaaacg tgggcttttt tctaacgaag ctggaatggg atccgcaccg aacgctggag
     1501 caaccgcaga tgtttctcat cctgtaaaac aaggtttagt acaaaccatc tctgttttta
     1561 ttgatactat gttaatttgt tccactactg cttttatgtt actaaattat gggacagaat
     1621 ctggattaac aggaatgccg tatgttcaac aagctatttt tgctgaagta ggtgaatttg
     1681 gtattcattt tataacaatt tctattttct tatttgcttt ttcttctttg attggaaact
     1741 attgctatgc tgaaagtaat tttaaattta ttattgacaa caaaaaagct ttatttattt
     1801 ttagaattat tactgtaatt ataattttct ttggtgccca agctagtttt aatactattt
     1861 gggatctagc tgatgttttg atgggcttta tggcaatcat gaatattgtc gtaattttat
     1921 tgttaggtaa aattgctttt aaatgtttaa aagactatag tattcaaaaa aaggaaggaa
     1981 aagatccaat tttccaccct gataatttag gaattaaaaa cgctgaattt tggcatgata
     2041 ttgaaaaaga atatgaaaaa cctgttgaag tatgatcctc aacaggtttt atttatccaa
     2101 atttgcttct aattctttta ttcgtgtatt aatactgtca atttcctgaa cgattgctgc
     2161 tgtcttttca tcaaaaatat tatcattact aataatttca taatacagtt tgactaattc
     2221 aataaacttc tcatctaaat cagcctgagc actttttata ctcactctcg ttttcgcgac
     2281 atccgctaat tcagatgatt tttcaattgt tttttctgtc agttttgctg cttttttact
     2341 tagatcatca aataatgcca tgattattcc tccttatatc tagctcatca cataattatt
     2401 ttaccacaac ctcagtaatc tacaatcacc ttttctctaa aactcttttt ctcaggatat
     2461 tataccctaa attttatcaa caagcatata attttatttt tttcatatat ttttgatgag
     2521 gtgattttat aaaaattcaa tggcagaaat taattatatg tattattatt cccctttttg
     2581 taggtactct ttcagcattt cttacacacg atgctatgac cacatttgaa acaattcaaa
     2641 agccagtgct atcaccaccg ggatggctat ttccaatcgt ttggactgtt ctatatattt
     2701 taatgggaat agcatcctat ctagtcttga ctacagagtc ttctcagcat gtaactcttt
     2761 tcatttataa gattcagctt tttttcaatt ttatttggcc tattatcttc tttaatctta
     2821 ggttgtactt acttgccttt atttggctaa taattttatg gctgcttatc ttaataacag
     2881 caatcctatt ttacaaaaca aataaattag ctggctatct aatgctccca tatcttattt
     2941 gggtaacttt tgctggttat ttaaatcttt cgagctatct tttaaattaa gttcaacaat
     3001 aaaaaaagcc atttactata tgtaaaaggc tttttgctat ttataaaaac tgccgaatat
     3061 caattactaa tttttcctca agattaatta atctttttgt aatatctttc gttttttcat
     3121 cagctgcttc atattgatta agatatttat taagagattt tactcccata ttgcatccat
     3181 cagttattag atcggcaatc gtttgatcag attcatgcat aaccaattta gcatttgtct
     3241 tgacccaaga catgccttta gccattggat taggttcttt gccttcatca tgaaacttat
     3301 ctaaaagagt ttgaatctct tcttttaact gctcatgttc tcttttgcag tcaattaaat
     3361 gctgtcttaa cgtttcatca tgtgcataat ctaaaacatc atcgattgaa gctgcaccca
     3421 tcttaatccc tgcatcacat tctcgtaata attttatagt atctggttca atcattattt
     3481 gtacgctcct tttattctat aaaatcatta tgctcttttt atttatattt attcttaatt
     3541 ataaggtaat aatccctctt tcaatatagc tggctactgc ttgttccact gtttctaagg
     3601 ttgtatattt aggtgaatat tctaacaatt tttgtgcatt ttcaatacta tattccccac
     3661 ttcttgcaat atgataataa gtatgatcta ttaattcatc atcattaaca tatttacacc
     3721 atttttccca tgacaaaaat ttaattttag gctcttgatt aaaataacgg tacattgctt
     3781 tagcataccc atataaagtc attgattctc tttcaaccgc atgaaaactc tctcctaatg
     3841 cttgatttct atgagtgatt gcctgataaa acatttgcgc aacatcatca ccatggacat
     3901 gatgtaaagt ttccatacct aaatttggta aatagatttc ctgcccattt gcaatttttt
     3961 gaaatactga aaagtctgta ttcccaagag gattaataat tgtccagcct ggtccagaaa
     4021 tttgacctgg cataattatc gttgctggaa aaccattttt gcgatactgt tcttttaaat
     4081 acaactcact ttgatattta tcaactccat aatcatccaa aggctctttt aaactatttg
     4141 gatcagctgg taaaaattct gctcgaccat gagcccaaat cgatgaacaa tataaataat
     4201 gagatatctt aatttccttc aatgctgtaa ccattttttt cgtatcttcg atattaaagt
     4261 tgattaagtc tacaacaata tcagcattca ttttagcaac tttataagca aagtcttcat
     4321 ctttattacg atctaaaact acttgttcta ccatcttcca agcaggatca tttatgtatg
     4381 gcttactttt accccgacta attgctacaa ctttaaaccc tcccttcact aatttaggca
     4441 ctaaatatga accgatatgt ccacatgccc ctataacaac tactttcata aatcttcctc
     4501 cttttgataa acctatctta attcaaataa agatgacatt aaagctagtt ttatcctttt
     4561 agaaaagctt tctcatttat ctttataaat acaagtatca tttgtaaaag ccttttacaa
     4621 accgctttgc ttaatccatt gcttatcata tttgttatga tagaatataa tagaaacgat
     4681 aataccagga gggcgagcaa tgctattaca agaaaaaatg aaaaacacaa aattttctcc
     4741 aaatgaagaa atagttattg attttatctt aacaaaacaa gagcgaataa aagattattc
     4801 gacaacgatg attgctggtg aaacatatac ttcaccatcg ctattggtta gaatttctaa
     4861 aaaattagga ttcagtggtt ataataattt taaaaacgcc tttttagaag aagttcaata
     4921 tttgcacaaa agtcatttaa atatagatgc caatcaacct tttttaaaaa cagattcaat
     4981 tatgaatatc gctaacaaaa taacacagtt aaaaactgaa agtcttaatg ataccctttc
     5041 attaattcat cacgatacac tgcaaaaagc agtgagagca ttacaaaaaa gtaatactgt
     5101 taaagttttt ggtgtttcta atttatcatt tccagctgaa gaatttgttt ttaaattacg
     5161 ccacatcggt aaaaatgctg aagtttttgt gttaaatagc aatatctatc aagaagcaat
     5221 gatggccaat tctaatgatt taggaatttg tctttcatat tctggagaat ctggtgaaat
     5281 tattaaaatt gctaatattt taaagaaaaa gaatgtccct attatcgcaa taacaagtat
     5341 tggtgaaaat agtttaactc gattagctga tattgtttta cgggttacaa caagagaaaa
     5401 atcatattca aaaattggtg cttattcttc actagaatcc atctcactta ttttagatgt
     5461 attatattct tgctttttta caacagccta tgatcagcat tatactttta aaactgaact
     5521 cgctaaaaat acggaatcac gagaaattac taatttaatt attcaagaaa atgaacaaaa
     5581 tgaaaaagaa tgacttttaa caaaagtcat tctttcctat tatttaagct gatgcaaacg
     5641 tgaatattct tgagcaatta gtccggcaat aaattgtaat tgataaggat gactgttttg
     5701 atcttgtaac gatttaaata aaattgttcg atcaatacat tcaggatact ctctgtcatc
     5761 actcgatatc atccatattt taggcgctgt taatcttttt ttcaaagctc ttaaatcttg
     5821 ataatcaaaa tatgcacctg tatacgaaaa aattataatc aaatcatctt catcagccgc
     5881 aacaatatat tgaatttgtt gagaatagga gatattagta tatacctgtt tattcagcat
     5941 taataagtca ccttgcaaat taaaagctac tgctccagct tttaacaaac caaaaatggc
     6001 taccttttga tattcattga tatccttaca taaatcaatt actgcatcca tatcaattga
     6061 tttttcaacc atgataatac tttctcttac tttatgacta taatcatgca gtcttgcttg
     6121 tttcgaagtt gcgtgtgaat gatcttcaaa agataaatct gttgacaaca acagttcttt
     6181 taattcagca aagtccctta aacctacttc
//
LOCUS       DAOMTA010000063         6201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_219_length_6201_cov_2.533518, whole genome shotgun sequence.
ACCESSION   DAOMTA010000063 DAOMTA010000000
VERSION     DAOMTA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219_length_6201_cov_2.533518"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PLK68_02815"
     CDS             <1..916
                     /locus_tag="PLK68_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89374.1"
                     /translation="GTLSAGGSAFESTYWGGSGLITAMIAGAIAINIINICLKKNIVI
                     KLPDSVPPAISDSFSAIIPYFFITIVCWGIRTLAGINIPVLVGEMLLPVLGAADNVFV
                     YSLQQFLSALLWMCGLHGDNITGAVTNVFTNQWLADNNTAFTAGTLVKDLPYVWTPNL
                     CRLSQWVSSCWPILIYMFMSSKKLPHLKPLATICLPPAIFCIIEPIMFGLPVVMNGFL
                     LIPFILTHTLTGALTYWLTSIGFVGKMYMSLPWATPSPILGYLSAGGSVGGIIIVFIN
                     LAIGLVIFYPFWKAYEKAEVAKMNTAEA"
     gene            979..1398
                     /locus_tag="PLK68_02820"
     CDS             979..1398
                     /locus_tag="PLK68_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM89375.1"
                     /translation="MECPYCHKEIPQDSAFCYHCGKEISADALKQKNKSKLKKNPREN
                     SWAKLGILLFFIGLIGLDFIAGTIFSAVGGNVKIPYILSSFAYLGAIVCGVLSLRVDK
                     QDRKKGFEPNGNKNYAWVSIVISGFVSLVNFSQVILK"
     gene            1415..1879
                     /locus_tag="PLK68_02825"
     CDS             1415..1879
                     /locus_tag="PLK68_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM89376.1"
                     /translation="MYCRKCGAILKDSAKFCDSCGSEVIKVKQRSYAQKYNDNKIKQK
                     MSKKDIERMEKHRDEKNPYIGAALFASVLALILAIVPWNYFGDGIGTSLPMRIVIVVF
                     ALLGDYHVTKAKQVNNLIYSKYGFRIKANIVSLANCLSIFVTVIGLFALFTL"
     gene            1916..2842
                     /locus_tag="PLK68_02830"
     CDS             1916..2842
                     /locus_tag="PLK68_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HRM89377.1"
                     /translation="MGVLFFKPIPRLAIWGHTLVKDYFGYHDFPDGVGQAWSFSCQKG
                     ASNICETAPYQGKTLLELWQEHQELFGHPNEQFPVIISLVGPEDDLSIQVHPDVKAAL
                     KLGYQAGKNEAWYFIEAEPGAKIVYGHNAKDEADLTHYIKEEQWDKLIRHLEVHPDDF
                     VYLPAGLLHALKKGSVVYEIQQATDVTFRFYDYHRKDANGNERELHLKEAIECLSYDQ
                     KEMENKLTTVMTSLENGEQTVFIDNDSFTVTKLELTGENHYYHDNYQLATVVRGSGTV
                     DGVPIKVGDNFLIPQGNKIVFDGHMTIMMTTR"
     gene            2854..4272
                     /locus_tag="PLK68_02835"
     CDS             2854..4272
                     /locus_tag="PLK68_02835"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM89378.1"
                     /translation="MKEEFLWGGALSNVQAEGAYLEDGKGLNVYDTLVVTPEPGIEPM
                     FCDTKVATDHYHHYQEDIDYMAQMGYKAYRFSVVWSRIHPLGNEESPNEAGLAYYEKM
                     VDYLLSKGIEPVVSLVHFDMPDYLLKNYNGFMNKEVINFYARHVEAVVSRLKGKVKYW
                     LTYNEINLAYCQSDLVAGAYLPDGMSKAEMFVQLNINVQIAHARAVEVIKRVDPQAKV
                     GGMIGHAPFYPLTCKSSDIIAADFKNKLHNYFAFDTMCQGELPDYFKQYALNRNITIS
                     LNKADEEVIKSASTKLDYLAFSYYRSSVQATFDEIDDVITLEDAILFDQRNLKNPYYQ
                     ANEWGWQIDSQGLRYSLIDFYHRYHKPLFIVENGIGIDEQLIDQKVYDDQRIDYYQQH
                     IKAIKQAVEHDGVDLIGYLAWSPIDFLSSHKEIRKRYGFVYVDRDFEDLKDLKRYPKK
                     SFYWYKKCIATNGDDLENNIEY"
     gene            4295..4591
                     /locus_tag="PLK68_02840"
     CDS             4295..4591
                     /locus_tag="PLK68_02840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM89379.1"
                     /translation="MFLLVCAAGMSTSLLVNRMKEAAETKEIEFQIEAHPVGQIEKYG
                     EAADVILLGPQVRYELKNVKAKYPNKPVEVINMQDYGMMNGNKVLDTALKLIEG"
     gene            4628..6094
                     /locus_tag="PLK68_02845"
     CDS             4628..6094
                     /locus_tag="PLK68_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89380.1"
                     /translation="MSFPKGFLWGGSISAAQIEGGWNEGGKSPVLVDYCTAGSAKERR
                     QIWYLDQEGKKVHKDWEAVDELPEGCKFQLFDDLHYTNHVASDFYHRYKEDIALLAEM
                     GYTTFNTSISWARIYPHGIKGGVNQEGVEFYRSVFEECRKYGMDPVITLYKYDEPISL
                     LEQHGGWRNRAMIDEFVEFARVCFTEYKDLVNKWMTFNEINIIMPLNDTPKDKAKRDL
                     AYLHNQLVAAARATVVAHEIDKDLKVGAMICGNMNYPLTPDPKDAFAQYKRFQDFFGY
                     SADTQIRGEYPPYAKRIWEEWNFTPEITEQDKKDLKEGKADFLGFSYYASGVITTHNE
                     EQLDITGGNILGSVKNPYLEANAWGWQIDPLGFKHFLHILNDRYQVPLFDVENGIGLI
                     ETEGEDGICHDSARIDYHRRHIQCMKEAVEEGVNLFGYTTWGCIDLVSAGTGQMDKKY
                     GFIYVDMDDQGNGDLHRSRKDSFYWYKKVIASNGEDLD"
BASE COUNT         2070 a          792 c         1288 g         2051 t
ORIGIN      
        1 tggaacttta agtgctggag gcagtgcttt cgaaagtact tattggggtg gttcaggttt
       61 gattacagca atgatcgctg gagcaattgc aattaatatt attaatatat gtttaaaaaa
      121 gaatattgta atcaaattgc ctgattcagt accacctgct atctcagata gtttctcagc
      181 aattattcct tatttcttta ttacgattgt atgttgggga attagaactt tagccggaat
      241 caatattcca gtattggttg gagaaatgtt attaccagtc ttaggagcgg cagacaatgt
      301 ctttgtttat agcttgcagc aattcttatc agcattgcta tggatgtgtg gtctacatgg
      361 tgacaatatt actggtgcag ttactaatgt ctttacaaat caatggcttg ctgataacaa
      421 cactgctttt actgctggaa cattagttaa agatttacca tatgtttgga caccaaactt
      481 atgtcgtcta tctcaatggg tatcatcttg ctggccaatt ttaatttaca tgtttatgtc
      541 tagtaagaaa ttacctcatt tgaaaccatt agcaacaatc tgtttaccac ctgcaatttt
      601 ctgtattatt gaaccaatta tgtttgggtt accagtagta atgaatggtt tcctattaat
      661 tccatttatt ttaactcata cattaacagg ggctttaact tattggttaa ctagtattgg
      721 ttttgtagga aaaatgtata tgtcattgcc ttgggcaaca ccttcaccaa tcttaggtta
      781 tttatctgct ggtggttcag ttggtggtat cattattgta tttatcaatc tagcaatcgg
      841 attagttatc ttctatccat tctggaaagc ttatgaaaaa gctgaagttg ctaaaatgaa
      901 tactgctgag gcatagaatt aattaattaa gaaaacttta acaagttctc ttaattaaaa
      961 aataaggagg aactaattat ggaatgtcca tattgtcata aggaaatacc acaagattca
     1021 gctttctgtt atcattgcgg taaagaaatt agtgcagatg cacttaaaca aaaaaacaaa
     1081 tcaaagttaa aaaagaatcc ccgtgaaaat agctgggcta aattagggat cctcctattt
     1141 tttatcggtt taattggatt ggattttatc gcaggaacaa tatttagtgc agttggtgga
     1201 aatgtaaaga ttccatatat tcttagttcg tttgcttatc ttggggcaat cgtttgtggt
     1261 gtattgtcat tgcgggtaga taaacaggat cgaaaaaaag gattcgaacc aaatggtaat
     1321 aaaaattatg cttgggtatc gatcgttatt agtggttttg ttagtttagt taatttttca
     1381 caagttattt tgaaatagag agcaggtgga tattatgtat tgtcgaaaat gtggtgctat
     1441 acttaaagat tctgcaaaat tttgtgatag ctgtggtagt gaagtaatta aagttaaaca
     1501 acgtagttat gctcaaaaat ataatgataa taaaataaaa caaaagatgt ctaaaaaaga
     1561 tattgaacga atggaaaagc atcgagatga aaaaaatcca tatattggtg cagctttatt
     1621 tgcttcagtt ttagcactta ttttagctat cgtaccatgg aattattttg gtgatggaat
     1681 tggaacaagt ttaccaatgc gaatcgtaat cgttgttttt gctttgttag gtgattatca
     1741 tgtcactaag gcaaaacaag taaataattt aatttatagt aaatatggtt ttagaatcaa
     1801 agctaatatt gtctcattag ctaattgctt atcaatcttt gtaaccgtaa ttggtttatt
     1861 tgctttgttt acactatagg catattaatg cctatttttt ataaggagaa aaattatggg
     1921 tgtattattt tttaaaccga tcccgcgctt ggctatatgg ggacatacgt tagttaaaga
     1981 ttattttggc tatcatgatt ttccggatgg agttggacag gcttggtcat ttagctgcca
     2041 aaaaggggct tcaaatattt gtgagactgc gccatatcaa ggaaaaactt tattagaatt
     2101 atggcaggag catcaagaat tatttggtca tcctaatgaa cagtttccgg ttattatttc
     2161 gttagtagga ccagaagatg atttaagtat ccaagttcat cctgatgtaa aagcggcttt
     2221 aaagttaggt tatcaagcag gcaaaaatga agcttggtat tttattgaag ctgagccagg
     2281 agccaagatt gtttatggac acaatgccaa agatgaagct gatttgactc actatatcaa
     2341 ggaagaacag tgggataaac tgattcgtca tttagaagtc catcctgatg attttgtata
     2401 tcttccggct ggattattac atgcattaaa aaaggggagt gttgtttatg aaatccaaca
     2461 ggctacagat gttactttcc gtttttatga ttatcatcgt aaagatgcaa atggcaatga
     2521 acgtgaattg catttaaaag aagcaattga atgtttgtct tatgatcaaa aggagatgga
     2581 aaataagctt acgactgtaa tgacatcatt agaaaatggt gaacaaactg tttttattga
     2641 taatgactca tttactgtaa caaaactaga acttacaggg gagaatcact attatcatga
     2701 taattatcag ttggcgacgg ttgtgagggg ctctgggact gttgatggag tgcctattaa
     2761 agtcggtgat aatttcttga ttccgcaagg aaacaaaatt gtttttgatg gtcatatgac
     2821 tattatgatg acaacgagat aaaggagaag atcatgaaag aagaattttt atggggcggt
     2881 gctttaagca acgttcaagc tgaaggcgca tatttagaag atggtaaagg gttaaatgtt
     2941 tatgacactc tagtagttac tccagaaccc gggattgaac cgatgttttg tgatacaaaa
     3001 gtagctactg atcattatca tcattatcaa gaagatattg attacatggc tcaaatggga
     3061 tataaagcat atcgcttttc agtagtatgg tcaaggattc acccgcttgg taatgaagaa
     3121 agtcctaatg aagcagggtt agcttattat gaaaagatgg tcgactatct tttatcgaaa
     3181 ggaattgaac cagtagtatc tttagttcat tttgatatgc cggattatct gttaaaaaac
     3241 tataatgggt ttatgaacaa agaagtaatt aacttttatg ctcgccatgt tgaagcggta
     3301 gtttctagac tgaaaggaaa agttaaatac tggctgacat ataatgaaat taatttagct
     3361 tattgtcaaa gtgatctggt ggcaggagct tatttaccag atggaatgtc taaagcagag
     3421 atgttcgtac aattaaatat taacgtacaa attgcacatg ctcgtgctgt agaggtaatt
     3481 aaacgtgttg atccacaagc caaagtaggt ggtatgattg ggcatgcacc attctatcca
     3541 ctcacttgta aaagtagtga tattattgct gcagatttta aaaataagtt acacaactat
     3601 tttgcttttg atacgatgtg tcaaggagaa ctaccagatt attttaagca gtatgcacta
     3661 aatagaaata taacaatcag tctaaataaa gctgatgaag aggtaattaa atcagcatcg
     3721 accaaactgg attatctagc attttcatat tatcgcagta gtgttcaggc tacatttgat
     3781 gaaattgatg atgtgattac tttagaggat gctattttat ttgatcagcg caatcttaaa
     3841 aatccttatt atcaagcaaa tgagtggggc tggcaaattg attcacaggg attacgatat
     3901 tcgttaattg atttttatca tcgatatcat aaaccattgt ttatcgtaga aaatggtatt
     3961 ggaattgatg aacagttaat tgatcaaaaa gtatatgatg atcaacgtat tgattattat
     4021 caacagcata tcaaagcaat taaacaggct gttgaacatg atggtgttga tttaattggt
     4081 tatttagctt ggagtccaat tgatttttta agtagtcata aagaaattag aaaacgttat
     4141 ggatttgttt atgttgatcg tgattttgaa gacttaaaag atttaaaacg ttatcctaaa
     4201 aaatcgtttt actggtataa aaaatgtatt gctaccaatg gtgatgattt agagaataat
     4261 attgaatatt aggctagagg gggaataatg atgaatgttt ttgttagtgt gtgcagcggg
     4321 aatgtcaaca agtctattag ttaatagaat gaaagaggct gccgaaacaa aagaaattga
     4381 atttcaaatc gaagcccatc ctgttggaca aattgaaaaa tatggtgaag cagctgatgt
     4441 tattttatta ggaccacaag tacgttatga attaaagaat gttaaggcta aatatccaaa
     4501 taagccggtt gaagttatta atatgcaaga ttatggaatg atgaatggta ataaggtttt
     4561 ggatactgct ttaaaattga ttgaagggta ataagattaa attaaaataa attttaggag
     4621 gtttattatg agttttccaa aaggtttttt atggggtggt tcaattagtg ctgctcaaat
     4681 tgaaggtggt tggaatgaag gtggaaaatc accagtatta gtagattatt gcacagctgg
     4741 ttcagctaaa gaacgtcgtc aaatttggta tttagaccaa gagggtaaaa aagtgcataa
     4801 agactgggaa gcagttgatg aattaccaga aggatgtaag tttcagttat ttgatgattt
     4861 acattatact aaccatgtgg ctagtgattt ttatcatcgt tataaagaag atattgcctt
     4921 gttagcagaa atgggatata caacatttaa tacatctata tcatgggctc gtatttatcc
     4981 tcatggtatt aaggggggtg ttaatcaaga aggcgttgaa ttctatcgta gtgtttttga
     5041 agaatgtaga aagtatggta tggatccagt tattacactg tataaatatg atgaaccgat
     5101 ttctcttctt gaacaacacg gtggttggcg caatcgggca atgattgatg aatttgttga
     5161 atttgcacga gtttgtttta ctgaatataa agatttggta aataaatgga tgacatttaa
     5221 tgaaattaat atcattatgc cattaaatga tactcctaaa gataaagcta aaagagatct
     5281 agcttatttg cacaatcaat tggttgcggc tgctagggca actgttgttg ctcatgaaat
     5341 tgataaagat cttaaagttg gtgcaatgat ttgtggaaat atgaattatc cacttactcc
     5401 tgacccaaaa gatgcatttg cacaatataa acgtttccaa gatttctttg gatatagtgc
     5461 agatactcaa ataaggggag aatatccacc ttatgctaaa agaatttggg aagagtggaa
     5521 ttttactcca gaaattacag aacaagataa aaaggattta aaagaaggaa aagcagactt
     5581 tttaggattt tcttattatg cttcgggggt aattactact cataatgaag aacaattgga
     5641 tattactgga ggaaacattt taggaagtgt taaaaatccg tatcttgaag caaatgcttg
     5701 gggttggcaa attgatccgt taggatttaa acatttcttg cacattttaa atgatcgtta
     5761 tcaagtaccg ttatttgatg ttgaaaatgg tattggactg attgaaactg agggtgaaga
     5821 tggtatttgt catgattcgg caagaattga ttatcatcgt cgacatattc aatgcatgaa
     5881 agaagcagtc gaagaaggtg taaacctatt tggttataca acttggggat gtatcgactt
     5941 agtaagtgcg ggtaccgggc aaatggataa aaaatatgga tttatctatg ttgatatgga
     6001 tgatcaagga aatggtgatc tacatcgctc aagaaaagat tcattctatt ggtataagaa
     6061 ggtaattgcc agcaatggtg aagatctaga ttagagtcag gagaaagtta tgaaaattgg
     6121 aattggaaat gatcatgttg cggttgaata taaaaatgtg atcagagagt atattgaagc
     6181 aaaatatggc tatgaagtaa t
//
LOCUS       DAOMTA010000064         6179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_223_length_6179_cov_2.666395, whole genome shotgun sequence.
ACCESSION   DAOMTA010000064 DAOMTA010000000
VERSION     DAOMTA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223_length_6179_cov_2.666395"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLK68_02850"
     CDS             complement(<1..469)
                     /locus_tag="PLK68_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89381.1"
                     /translation="METYLEYYIDATDDSNFAFQLSSPEVIEKTDRLSSNIIDWSQAK
                     NSHFKNEYINDAGLKYTWAFEFYDMQGFPRATICLNRIKNMPFTKEEQEIITLALPLL
                     INQYRNYFYEEVKDQNLQSIWEKYQLTSREKEIADMLCKGMKTERISKLLSISI"
     gene            complement(552..677)
                     /locus_tag="PLK68_02855"
     CDS             complement(552..677)
                     /locus_tag="PLK68_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89382.1"
                     /translation="MDADLTYSVYQKIYNLILSCSHIRSPKAFLETLAKEIKSLC"
     gene            815..1693
                     /locus_tag="PLK68_02860"
     CDS             815..1693
                     /locus_tag="PLK68_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM89383.1"
                     /translation="MGEFNYYSKKVCVVTGASSGMGKATTKLLVENGAEVYAMDIQPV
                     DVEGIAKFVQVDLSDKASVDQAFTELPEKIDAFFGVAGLLGQNPPFIKVACIDLVSNK
                     YMMEELLPERMNENGAIALVSSGVGVGWEKEGNKKYYVDVVEAEGWDNTVKAIEAKGI
                     TAFNGGLAYVYAKLAVNYLVAKMQKFYGLRKVRVNAIMPGGTSTSFGSESGLDVNDSS
                     LDSESGYCGYTGRIADPVEMAKPLLFLNSDLASYVSGAILPVDSGTTSEIAAGIRPNP
                     VGDNLDLVYSAIFQAK"
     gene            1896..5786
                     /locus_tag="PLK68_02865"
     CDS             1896..5786
                     /locus_tag="PLK68_02865"
                     /inference="COORDINATES: protein motif:HMM:NF019941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM89384.1"
                     /translation="MNIRKLFKKTMICLTSAFIVGSTCLSGVSAATYTTGSIHHDDWV
                     LTKPDGTTHGHEGQLQVNGSDAYCVDYETTFHKNKTVVAGTYKDIGLTAEKAKRLAVI
                     AYYGHKVSGRTGTDWYAITQGLLWREIEGRTDLEFVSCKTAPTYADQQKCWNAILADV
                     DRYYTAPSFAKNTYTVDADGSITLTDTNNVLSDMVIANDGGLDVSISGNKLTIKGKSG
                     VTGKVNLVLKKNIPVAETGTTIVYTADDCQAVAQFKISDPFQASLSVEIQEYGNLELT
                     KYNEDKSGLVPNTEYRITGVGYDQTFTTDANGKISIERLKVGSYKITEVKASEGYLIN
                     TGSMTVTVEANKTAKAEFTNNEPTGKVTFTKSIDLNQTNNLKGDANVEGVTYQLVADE
                     KITNKAGTKTFFNKGDVVESKKTDANGKITWDDLPLGHYVIKETKTNGTLVLNEETYK
                     VSIEYEGQTVAKVSRMAEGSDRVNMQKIQIFKSGTKDGISDLVKGLQGAEFTFKLKSE
                     VDHVGWDSAPAYAIVVTDKNGKANTSYLPYGTYLVRETKTPADYITAPDFTVSVTKDY
                     TEFDDVEQVKRINVNNRPFTSQLKIVKKDKETGKTVTLNSASFKIKDANGNYVTQKVS
                     GKKYDTFTTNSKNQVVVFDGNNGEVFLPLSLDAGTYSVEEVKVPEGFLELESPVTFTI
                     TNTRNYDVDGDEDPIVTVQVKNDRCYGKLEVKKSIESFDADKTLVNKEDLSGFEFSLY
                     AKKDIVSPIDGSVLLKANEKYGTYLTDKEGKISVSKIPMGSYILKETKTKDGFALDDK
                     ETTITFKQTDTVTKEYTVTADVENKTTKYEFSKKSVTGDDELEGAKLTVTDKDGNIID
                     EWTSGKETHMIEGLTVNQVYTLTEEIAPDGYVKATTIYFTVENDGNVQKITMIDKTVS
                     MSKVDVGGKEVEGATIQVKDKDGNIVDEWVSTTESHLIKGLEEGQTYFLHEEVSADGY
                     VKATDVEFTVSKEKTDDKVTMIDKIVDMSKIDVAGEEVPGAKIQVFDEDGNIVDEWIS
                     TDEPHKIKGLEEGKKYILHEEVGPEGYVVATDVEFTVTEEKENQHLELTDKQLFIKKM
                     DQLDKYVEGAKLQVLDKEGNVIAEWNTKEDYAVSGLKAGETYILHEVEAPENYIKAED
                     IEFTVKDDKENQYVEMIDIKTDDVDITKYDATSKKELPGAHLEIRDKDGKLIEEWIST
                     EEQHKVRLVVGETYTLTETIAPDGYKVAETIEFTVEDNGKVVQQVQMFDELLPARQIV
                     NTGDNSNIMIYVLLASGSAVALLGTYYFERKKEE"
     gene            5845..>6179
                     /locus_tag="PLK68_02870"
     CDS             5845..>6179
                     /locus_tag="PLK68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM89385.1"
                     /translation="MKEGILICGLNGAGKSTIAKQLATHLNYTYLDVEDFYFPVKNDY
                     KNIRSKQEMEAHLLGAIQETQPFILASVIGDFNEEILSYITEIIYIEVPKDIRIERMK
                     QRSINQFGP"
BASE COUNT         2345 a          799 c         1193 g         1842 t
ORIGIN      
        1 caatggaaat ggataacagc ttactgattc tttcagtttt catgccttta cacaacatat
       61 ctgcaatttc tttttcacga gatgtaagct gatatttttc ccaaattgat tgtagatttt
      121 gatcttttac ttcttcgtag aaatagttac gatattgatt aatcaaaagt ggcaaggcaa
      181 gggtaatgat ttcctgttct tcttttgtaa atggcatatt tttaatacgg ttgaggcaga
      241 tggttgcacg agggaaacct tgcatatcat agaattcgaa agcccaggtg tactttaaac
      301 ctgcatcatt aatatattcg tttttgaaat gtgagttttt tgcttgactc caatcgataa
      361 tatttgaaga taatcgatct gttttttcaa taacttcagg tgaagacaac tgaaatgcaa
      421 agtttgaatc atctgttgca tcgatgtaat attccagata ggtttccatc catcctggct
      481 tagtgccatc actaaatgta ttggcgattc ttcctgctgt atcataataa aaaatgacga
      541 cactatcata atcaacatag tgattttatt tcttttgcca gtgtttctaa gaaggcttta
      601 ggtgaacgta tatggctaca agataaaatc aaattgtata ttttttgata aactgaataa
      661 gttaaatcag catccatgat taaaacctcc tttcaaaact ctataaacaa aattatacac
      721 catacataaa aatacgcaat tatgagtatt taaagtttac tagtccactt tgtataattt
      781 atatatcaga taatgaaaaa aaggagacaa aaaaatgggt gaatttaatt actattcaaa
      841 aaaagtttgt gttgtaactg gtgcttcttc tggtatggga aaagcaacta ctaagttact
      901 tgtagaaaat ggtgcagaag tatatgcaat ggatattcaa ccagtagatg ttgaaggtat
      961 tgcaaaattt gtacaggttg atttaagcga taaagcatct gttgatcaag cgtttactga
     1021 attgccagaa aagattgatg ctttctttgg agttgcaggt ttgttaggac aaaatcctcc
     1081 atttattaaa gtagcatgta tcgatttagt atctaacaaa tacatgatgg aagaattatt
     1141 accagaacgt atgaatgaaa atggtgctat cgcacttgtt tcatcaggcg taggtgtcgg
     1201 atgggaaaaa gaaggtaata aaaaatacta tgttgatgtt gtagaagctg aaggttggga
     1261 taatacagtg aaagctattg aagcaaaagg tattacagca tttaatggtg gtctagctta
     1321 tgtttatgca aaattagctg taaactactt agttgcgaaa atgcaaaaat tctatggttt
     1381 aagaaaagta agagtgaatg cgattatgcc aggtggaaca agtacttcat ttggttcaga
     1441 atctggatta gatgtcaatg attcttcatt agattcagaa tcaggatact gtggttatac
     1501 aggaagaatc gctgatccag ttgaaatggc aaaaccatta ttattcttaa acagtgattt
     1561 agcttcatat gtatcaggtg ctattctacc tgtagattca ggaacaacga gtgaaattgc
     1621 tgcaggtatt agaccaaacc ctgtaggaga taatttagat ttagtatata gtgctatctt
     1681 ccaggcaaaa tagactatca acaaaacaaa ataatgacat gaaatgatat tcctactaga
     1741 aaatggtagg aatatttttt atttacaggg taaatttacc ttgtggaaag gaaattatga
     1801 aaaatagaat aagaagtccg tgaaggatat gtatgaacag tatttattga tgctgttctt
     1861 tttttatgca aattaaaacg aaaggatgat taaaaatgaa cattagaaaa ttatttaaga
     1921 agacgatgat atgtcttacc agtgcattta ttgttggatc gacatgttta agtggtgtat
     1981 ctgctgctac ttatacaaca ggttcaatcc atcatgatga ctgggtactt acaaaaccag
     2041 atggcacaac tcatggccat gaaggtcagt tacaagtcaa tggcagtgat gcctattgcg
     2101 tggattatga aacgacattc cacaaaaata agacagtagt tgcaggaacc tataaagata
     2161 ttggattaac tgctgaaaaa gccaaaagat tagcagttat tgcatattat ggccataaag
     2221 tgtctggaag aactggtact gactggtatg cgattacgca aggattactt tggagagaaa
     2281 ttgaaggtag aacagattta gaatttgtat cttgtaaaac tgctccaact tatgctgatc
     2341 aacaaaaatg ctggaatgct atcttagctg atgtggaccg ttattatact gctccatcat
     2401 ttgcgaagaa cacttataca gtagatgcag atggatctat tacattaaca gatacaaata
     2461 atgtattaag tgatatggta attgctaatg atggtggact agatgtaagt atctcaggta
     2521 ataaacttac aattaaaggt aaaagtggtg ttactggaaa agtaaatcta gtattaaaga
     2581 aaaatattcc agtagcagaa actggaacta caattgttta tacagcagat gactgtcagg
     2641 ctgtagctca gtttaagatt tcagatccat tccaagcttc attaagtgta gaaattcaag
     2701 agtatggaaa ccttgaattg actaaatata atgaagataa aagtggatta gttcctaata
     2761 ctgaatatcg tattacaggt gttggttatg atcaaacatt cactactgat gcaaatggta
     2821 aaatcagtat tgaacgatta aaagttggat cttacaagat tactgaagta aaagctagtg
     2881 aaggttattt aatcaataca ggaagcatga ctgtaacagt tgaagcaaat aaaactgcga
     2941 aagctgaatt tacaaacaat gaaccaacag gtaaagttac cttcacaaaa tctattgatt
     3001 taaatcagac aaataattta aaaggtgatg caaatgttga aggtgtcact tatcagttag
     3061 tagctgatga aaagattaca aataaagctg gaacaaaaac attctttaat aagggtgatg
     3121 ttgtagaaag caagaaaact gatgcaaatg gaaagattac atgggatgat cttccattag
     3181 gacattatgt gatcaaggaa acaaaaacta acggaacact tgtattaaat gaagaaactt
     3241 acaaggttag tattgaatat gaaggacaaa cagtagctaa agtttcgaga atggctgaag
     3301 gatcagatag agtcaatatg caaaagattc aaatcttcaa gtcaggaact aaggatggta
     3361 tctcagatct agttaaagga ttacaaggtg ctgaatttac atttaaatta aaatcagaag
     3421 tagatcatgt tggatgggat agtgcacctg cgtatgcaat tgttgtaact gataaaaacg
     3481 gtaaggcaaa tacatcatat ttaccttatg gaacatattt ggttagggaa actaaaactc
     3541 ctgcagatta tattactgca ccagacttca cagtttcagt aacaaaagac tatacagagt
     3601 ttgatgatgt tgaacaggtg aaacgaatta atgtcaacaa cagacctttc acatcacaat
     3661 taaaaattgt taaaaaagat aaggaaacag gtaaaactgt tacattaaac agtgcatcat
     3721 ttaaaattaa agatgcaaat ggtaactacg ttactcaaaa agtatcaggt aagaaatatg
     3781 atactttcac aacaaattca aaaaatcagg tagttgtatt tgatggtaat aatggggaag
     3841 tattcttacc attatcttta gatgctggaa cttacagcgt agaagaagtt aaagtacctg
     3901 agggattcct agaattagaa tcaccagtta cattcacaat tacaaacact agaaactatg
     3961 atgttgatgg tgatgaagat ccaatcgtta ctgttcaggt taaaaatgac agatgttatg
     4021 gtaagttaga agttaaaaag agtatcgaat catttgatgc agataaaact ttagtcaata
     4081 aagaagattt atcaggattt gagttctctt tatatgctaa aaaagatatt gtttctccaa
     4141 ttgatggaag tgtcttatta aaagcaaatg aaaaatatgg aacttactta acagataagg
     4201 aaggtaagat ttctgtctca aagattccaa tgggatcata tatcctaaag gaaacaaaaa
     4261 caaaagatgg attcgcttta gatgataaag aaacaaccat cacatttaaa caaacagata
     4321 cagtaactaa ggaatataca gttactgctg acgttgaaaa caagacaact aaatatgaat
     4381 tctctaagaa atcagttact ggtgatgatg aactagaagg tgctaaatta actgttactg
     4441 ataaagatgg aaacatcatt gatgaatgga cttcaggtaa agaaactcat atgatcgaag
     4501 gtttaactgt caatcaagtt tatacattaa ctgaagaaat tgcaccagat ggatatgtta
     4561 aagcaacaac tatttacttt acagttgaaa atgatggcaa tgttcaaaag attacgatga
     4621 ttgataaaac tgtatcaatg tcaaaagttg atgtaggtgg taaagaagta gaaggagcta
     4681 ctattcaggt caaagataaa gatggaaaca ttgttgatga atgggtatca actacagaaa
     4741 gtcatttaat caaaggcctt gaagaaggac aaacttattt cttacatgaa gaagtatcag
     4801 ccgacggata tgtaaaagct actgatgtag aatttactgt ttctaaagaa aaaacagatg
     4861 ataaagtgac aatgatcgat aagattgttg atatgtctaa aattgatgtt gcaggtgaag
     4921 aagtacctgg tgctaagatt caagtatttg atgaagatgg caatatcgta gatgaatgga
     4981 tttcaacaga tgaaccacac aagatcaaag gattagaaga aggtaaaaag tacatcttac
     5041 atgaagaagt tggtcctgaa ggttatgttg tagctactga tgtagaattc actgttacag
     5101 aagaaaaaga aaatcaacat cttgaattaa ctgataaaca gttattcatt aagaaaatgg
     5161 atcagttaga taaatatgta gaaggtgcta aattacaggt attagataaa gaaggtaatg
     5221 taattgctga atggaatact aaagaagatt atgcagtatc tggattaaaa gctggagaaa
     5281 catatatctt acatgaagta gaagcacctg aaaattatat taaagctgaa gatattgaat
     5341 ttacagttaa agatgataaa gaaaatcaat atgtagaaat gattgatatc aaaactgatg
     5401 atgtagatat cacaaaatat gatgcaacaa gcaagaaaga attacctggt gctcatttag
     5461 aaatcagaga caaagatggt aagttaatcg aagaatggat ttctacagaa gaacaacata
     5521 aagtaagatt agttgtaggt gaaacttaca ctctaactga aacaattgca ccagatggtt
     5581 ataaagtagc tgaaacaatt gaatttactg ttgaagataa tggtaaagtt gtacaacagg
     5641 tacaaatgtt tgatgaatta ctaccagcaa gacaaattgt aaatactggt gataattcta
     5701 atatcatgat ttatgtatta ttagcttcag gcagtgcagt tgcattactt ggaacatatt
     5761 attttgaaag aaaaaaagag gaataaataa ttcagaagtg ctataatttc aaatatcaac
     5821 attttgaatt cgaggtgatg acagatgaaa gagggcatat taatttgtgg tttaaatggt
     5881 gctgggaaaa gtacaatagc taagcaatta gcgactcatt taaactatac atatttagat
     5941 gttgaagatt tctattttcc agtcaagaat gattataaga acattcgaag caaacaggaa
     6001 atggaagctc atttattagg tgcaatacaa gaaacacaac cttttatact tgcaagtgtg
     6061 ataggggatt ttaatgaaga aatcttaagt tatatcacag agattattta tattgaagtt
     6121 ccaaaagata ttcgtattga aagaatgaag caaagatcga ttaatcaatt tgggccaag
//
LOCUS       DAOMTA010000065         6178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_224_length_6178_cov_2.859873, whole genome shotgun sequence.
ACCESSION   DAOMTA010000065 DAOMTA010000000
VERSION     DAOMTA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224_length_6178_cov_2.859873"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..3127)
                     /locus_tag="PLK68_02875"
     CDS             complement(257..3127)
                     /locus_tag="PLK68_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM89386.1"
                     /translation="MFKKKFFIGSLSLMFALSGIGAVQAEGDQGSKSTASKVVELDPN
                     GNAFLTDLTDDGYAELGRYKVEKIKDNIYHWDDGTKKLPGGATDESGVTNNPSSMYFV
                     LEEDGVLLVDLGNGSENDEDIQNAKTIVNSMTGNKPLSIIITHSHGDHTGFGRSETVF
                     ADVDVEKVYISEPDYAAAAEAITQFDSKITKVNHGDTVNIYGAPYVFNVVSAHTEGSL
                     MITDTTHGALFTGDTFGSGFIWALFETNGGNPIAALNEGCALARTILNENPSLSILAG
                     HRWQQFWEDNAQRPNEMSIQYFNDMAQVISGLLDGTTISQPYPEVAWAPDAIELSSNG
                     AKAKIDTLPKYVEAYKASVNEMSEAYVYSGSDKLSIESVNAAAAATFVVYPDGNLTDE
                     EAQKYINESGIKDIVDRAASKVYVARPANGISFTDDDVIGFETIVGKIGVSNNFKLIG
                     IGDGATFINQNLTSYMNFVSGLALINPAQGQEVKVSVPTYLVTDDQAVINSYVKANNA
                     GKVKDGYYENPESHYEIVVVNSDTKTSAADATKDAWNTVLEKFGRIGNYSEVYKQTAT
                     WYSRPLLSGDKEADQARKYQYFDSVDAIDNIERHVVTQDLDKDGTDSLWYEYIPEQSK
                     DAQPGSVPVVILFHGNTNDPRTQYDTSGWAQIASEEGVILICPEWQGHTYQGYTYDPM
                     TDDSNETPDSDVITMLKIIEEKYPQIDQSRIYISGLSAGSRNTTNAGLSDAKYFAAGA
                     GHSGPFGASDLNKEAVAANKDKYDMPIIFFTGDGDEYCKDAFDTTELNAGLQVAQLYQ
                     ELNDMEVTQVEDIKDEDAYLYGVPWTKRYTIEPTAENIAKIDVGAIENAKGVEISMAR
                     IYGWGHWNYTPDAKLMWEFMSKYARDLETGETIRLDLQEPDTPTDNPSDTPNDTEKPA
                     TQPSTSTSVKTGDSTMIAPFVIVASLSLFALVTVIKKSKAIR"
     gene            3502..4161
                     /locus_tag="PLK68_02880"
     CDS             3502..4161
                     /locus_tag="PLK68_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM89387.1"
                     /translation="MKILIIEDDLELREELKVLLDNNGYQGIILKDFKNALEEILVID
                     PDLILLDIKIPFLNGQQLLKKLREKSTIPVIMVTSKDSEIDEILALSYGADDYITKPY
                     NPTILLLHIEAVFKRLHKAEPKLTYQNIRVNLMKSTLEKDDQELLLSKNEMGIFYYLL
                     MNQGKIVTRDDIMNYLWGTDKFIDDNTLTVNMTRLRKRLEKIGLFDVIETRRDQGYML
                     I"
     gene            4158..5168
                     /locus_tag="PLK68_02885"
     CDS             4158..5168
                     /locus_tag="PLK68_02885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM89388.1"
                     /translation="MRIKEYLLDRMAYLVLLIIMIVSVLFFLLMFKVSNALIYAIVLV
                     MLLFSGLLIGYDYYQKHAFYNNFRIKLNQLDKKCLITELIAQPDFLEGKILYEALYEI
                     DKSMYEEIETYQDNLKNFKEYIELWIHEVKLPISSVKLMIHNHEHNERKLKDQVSRIE
                     NYVEQVLYYARQDSSEKDYLIKQCELGTIIRNVIKKNQDSLLYQNIKIEIGQVNCSVL
                     SDSKWLEFIINQIVSNSIKYRRTDKCKIAFNVIRRNNIALEIIDNGLGIKTSDLTRVF
                     EKSFTGENGRLVSSSTGMGLYIVKRLCDKLGHKIIIESKYSEYTKVTISFNDEQYYKV
                     VR"
     gene            5236..6003
                     /locus_tag="PLK68_02890"
     CDS             5236..6003
                     /locus_tag="PLK68_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89389.1"
                     /translation="MENILTIENISKYYGNKSNLTKAISNISMNVDKGEFIAIMGASG
                     SGKTTLLNVISTIDKVTSGHIYIEGQDITKLKGNDLNRFRREELGFIFQDFNLLDTLT
                     AYENIALALQIQNFKAKAIEQQIKVVARKLDIEDILNKYPYEMSGGQKQRVASARAIV
                     TNPKLVLADEPTGALDSKSSKMLLERLQRLNQDYQTTILMVTHDAFSASYASKVIFIK
                     DGKVFNQFNRGEATRKQFFDKIIDVVSLLGGDVSDVI"
     gene            5993..>6178
                     /locus_tag="PLK68_02895"
     CDS             5993..>6178
                     /locus_tag="PLK68_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89390.1"
                     /translation="MLFNLSLKNIRKSFKDYAIYFFTLILGVAIFYIFNSLESQTVML
                     KLNSSTKDIIKLMNNVLS"
BASE COUNT         2106 a          898 c         1001 g         2173 t
ORIGIN      
        1 gcagtaaaag taaagaaacc aagtaatgat gcaatcttaa aacaatttga agaaactgaa
       61 aaattattta ataaagtaat gaataaaggt aagtaaatta taggcaattg ctgtaattag
      121 tgcagttgct ttttattatt aaagaagata gctgtctgtt taatatgttt ttttagtcaa
      181 ttagggataa aaaaacaaag gtttttaata aacctttgtt ttttatattt ctacaattat
      241 ttaaaactgt atgtgattat cttattgctt tgcttttttt aataacagtt actaatgcaa
      301 ataatgataa acttgcaaca ataacaaatg gtgcaatcat tgttgaatca ccagttttaa
      361 cacttgtaga tgttgatggt tgagttgctg gtttttcagt atcgttagga gtatcactag
      421 gattatcagt aggagtatct ggctcttgta gatccaaacg aattgtttca ccagtttcta
      481 aatcacgagc atatttactc ataaattccc acattaattt agcatctgga gtatagttcc
      541 aatgtcccca accataaata cgagccatgc tgatttctac acctttggca ttttcaattg
      601 caccaacatc gattttagca atattttcag cggttggctc aattgtatag cgttttgtcc
      661 aaggaacacc atatagatat gcatcttcat ctttgatgtc ttcaacttga gtaacttcca
      721 tatcatttaa ttcttgataa agttgagcta cttgtaatcc tgcatttaat tctgttgtat
      781 caaatgcatc tttacaatat tcatcaccat caccagtgaa gaaaataatt ggcatatcat
      841 atttatcttt attagcagca acagcttctt tatttaagtc acttgcaccg aatggcccgg
      901 aatgaccagc tccagcagca aagtatttag catctgatag accggcattt gttgtatttc
      961 tacttccggc agataatcca gaaatataaa ttcttgattg atcgatctgt ggatatttct
     1021 cttcaatgat ctttaacatt gtaataacat ctgaatcagg agtttcatta gaatcatcag
     1081 tcattggatc gtatgtatat ccttgatatg tatgaccttg ccactcagga caaattaaga
     1141 ttacgccttc ttcacttgct atttgtgccc atcctgaagt atcatattga gttctaggat
     1201 catttgtatt accgtggaat agaataacta ctgggacact tcctggttga gcatctttag
     1261 attgttcagg aatatattca taccataatg aatcagtacc atctttatct aaatcttgag
     1321 taactacatg acgctcaata ttatcgattg catcaacaga atcaaaatat tggtattttc
     1381 ttgcttgatc agcttcttta tcaccagaaa gtaatggtct agagtaccaa gttgctgttt
     1441 gtttatagac ttcactatag ttaccaattc ttccaaattt ttctaaaaca gtattccatg
     1501 catcttttgt tgcatcagca gcacttgttt ttgtatcgct attaacaaca acgatttcat
     1561 aatgactttc tgggttttca taatatccat cttttacctt accagcatta tttgctttta
     1621 cataactatt gataacagct tgatcatcag ttactaagta agtaggaaca gatactttaa
     1681 cttcttgccc ttgagctgga ttaattaaag caagcccaga tacaaagttc atataagatg
     1741 ttaagttttg attaatgaaa gttgctccat caccaatccc aattaattta aagttatttg
     1801 aaacaccaat tttacctacg attgtttcaa aacctataac atcatcatca gtaaaagaaa
     1861 tgccattagc aggtctagca acataaactt tgcttgccgc ccgatcaaca atatctttta
     1921 ttcctgattc attaatatat ttttgtgctt cttcatctgt taaatttcca tcaggataaa
     1981 caacgaaagt tgcagcagcg gcagcattga ctgattcaat acttaattta tcgctaccac
     2041 tgtaaacata ggcttcgctc atttcattaa ctgaggcctt gtaagcttca acatactttg
     2101 gtaatgtatc tattttagcc tttgcaccat tgcttgaaag ctcaattgca tcaggcgccc
     2161 aggcaacttc tggataaggt tgtgaaatag ttgtaccatc taatagaccg ctaataactt
     2221 gtgccatatc gttgaaatat tgaattgaca tttcgttagg tctttgggca ttgtcttccc
     2281 aaaattgttg ccagcgatga ccagctaaaa tagatagaga aggattttca tttaaaatag
     2341 ttctagctaa agcacatcct tcgtttaaag cagcaattgg gtttccacca tttgtttcaa
     2401 aaagtgccca aataaatcca ctgccaaatg tatcaccagt aaataatgct ccatgagtgg
     2461 tatctgtaat cataagagag ccttcggtat gagcactgac aacattgaat acatatgggg
     2521 caccataaat gttaacagta tcaccatgat taacttttgt gattttacta tcaaattgag
     2581 tgattgcttc cgcggcagca gcataatcag gttcactgat atatactttt tcaacgtcaa
     2641 cgtctgcgaa tactgtttct gatcgtccaa agccagtatg atcaccatga ctatgagtaa
     2701 taataattga taacggctta tttcctgtca ttgaatttac gattgttttt gcattttgaa
     2761 tatcttcatc gttttcagag ccatttccta aatcaactaa taatacaccg tcttcttcta
     2821 gtacaaaata catagatgat gggttatttg ttacgccgct ctcatctgtt gcaccaccag
     2881 gaagtttttt tgttccatca tcccagtgat aaatattatc cttgattttc tctactttgt
     2941 accttcctaa ttcagcatat ccatcatcag taagatctgt taaaaatgca tttccatttg
     3001 gatcaagttc tactacttta gatgctgttg atttggatcc ttggtctcct tcggcctgta
     3061 ccgcaccaat accacttaat gcgaacatca ggcttaggga accaataaaa aattttttct
     3121 taaacatttc ttttttccct ccttcattat aaatatttta tcacagcggc tttatgaaag
     3181 cgattacaaa aactgatggt aagtatcata atttgatacc taatatcata atttgattga
     3241 tatttataca attaaatatg atataatcat taaataactt acttatttat aaattaaaac
     3301 tgttaaatag aaattctaca ctaataacga tatttagtaa ttatatgatt gagacatagg
     3361 atttatccta tgtctttttt tgttttaaaa ttatcgtagc gaatctttct aactaaaaaa
     3421 tgagataata ataacttaca tctttgtaag ataactatgt aataaaaaat aattgtgtat
     3481 aataaaaaag aggagatcgt tatgaaaata ttaattattg aagatgattt agaattacga
     3541 gaagaattaa aagttttatt agataataat ggttaccaag gaatcattct aaaagatttt
     3601 aaaaatgcgt tagaagagat tttagtaata gatccagatt tgattctatt agatattaag
     3661 attccatttt tgaatggaca gcagctatta aaaaaattaa gagaaaaatc aactattcca
     3721 gttattatgg ttacaagtaa agatagtgag atcgatgaaa ttcttgcatt aagctatggt
     3781 gctgatgatt atattactaa accatataat ccaacgattt tattactgca tattgaagct
     3841 gtttttaagc gactccataa agctgaacca aaactgactt atcaaaatat tagggttaat
     3901 ttgatgaaaa gtacgttaga aaaagatgat caggaattat tgttatctaa aaatgagatg
     3961 ggaatttttt attatcttct tatgaatcaa gggaaaatcg taactcgtga tgatattatg
     4021 aattatctat ggggtacgga taaatttatt gatgataata ctttgacagt taatatgact
     4081 agactaagaa aaagacttga aaagattggc ctttttgatg tgattgaaac aaggagagat
     4141 caggggtata tgttaatatg agaataaaag aatacttact agatagaatg gcctatctag
     4201 ttttattaat aattatgatt gtgagcgtgc tattcttctt attgatgttt aaagtttcaa
     4261 atgcattaat ttatgctatt gttttagtta tgcttttatt tagtggtttg cttattggat
     4321 atgattatta tcaaaaacat gctttctata ataattttcg cattaaatta aatcaattag
     4381 ataaaaaatg tttaattaca gaattgattg ctcagcctga ttttctagaa ggaaagattt
     4441 tatatgaggc attgtatgag attgataagt caatgtatga ggagattgag acttatcaag
     4501 ataatcttaa gaattttaaa gaatatattg aattatggat tcatgaagtt aaattaccaa
     4561 tttctagtgt aaagttgatg attcataatc atgaacataa tgaaagaaaa ctaaaggatc
     4621 aagttagtcg aattgaaaat tatgtagaac aagttttata ttatgcccga caagatagta
     4681 gtgagaaaga ttatttgatt aaacaatgtg aattaggaac aattattaga aatgttatta
     4741 agaagaacca agatagttta ttgtatcaaa atattaagat cgaaatcggt caagttaatt
     4801 gttctgtttt aagcgatagc aagtggctag aatttattat taatcaaatt gttagtaata
     4861 gtattaaata tcggcgtaca gataagtgta aaatagcttt taatgtgatt agaaggaata
     4921 atattgcttt agaaataatt gataatggtc tgggaatcaa gactagtgat ctaacgagag
     4981 tatttgaaaa atcatttact ggtgaaaacg ggcgtcttgt ttctagttct acggggatgg
     5041 gcttatatat tgttaagcga ttatgtgata aattaggtca taagattata attgaatcta
     5101 aatatagtga gtatacgaaa gtaacaattt cttttaatga tgagcaatat tacaaggttg
     5161 taaggtgatg taagttattt cgatgttacc aagaccgcct tttttctata atttaattgt
     5221 aaaggagaaa agattatgga aaatattcta acgattgaaa atattagtaa atattatgga
     5281 aataagagta atcttactaa agctatcagc aatatttcaa tgaatgttga taaaggtgaa
     5341 tttattgcta tcatgggggc tagtggttca gggaaaacta cactactgaa tgttatttca
     5401 actattgata aagttacaag tggtcatatt tatattgaag gtcaggatat tacaaaactt
     5461 aaaggtaatg atctaaatag gtttagacgt gaagaactag gatttatttt tcaagatttt
     5521 aatttattag atacgctaac agcttatgaa aatattgctt tggctttaca aattcaaaat
     5581 tttaaagcta aagcaattga acagcaaata aaagttgttg caagaaaatt agatattgaa
     5641 gatattttaa ataaatatcc ttatgaaatg tctggaggac aaaaacagcg tgtagctagt
     5701 gcgcgggcca ttgttacaaa tcctaaactt gttttagcag atgagcctac tggagcgtta
     5761 gattctaaaa gttctaaaat gttattagaa cgacttcaaa gattaaatca agactatcaa
     5821 acaacgattc taatggtaac tcatgatgct tttagtgcca gctatgcttc taaagtgatt
     5881 tttatcaaag atggaaaggt ctttaatcaa tttaatcgag gtgaagctac tcgtaaacaa
     5941 ttttttgata aaattattga tgttgtttct ttgcttgggg gcgatgtaag tgatgttatt
     6001 taatctttca ttaaagaata ttagaaaaag ttttaaagat tatgcaatct atttttttac
     6061 attaatcctt ggagtggcaa tcttttatat cttcaattct ttggaaagcc aaacagttat
     6121 gctgaaacta aatagttcaa ctaaagatat tattaaacta atgaataatg tgttgtca
//
LOCUS       DAOMTA010000066         6169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_225_length_6169_cov_2.401210, whole genome shotgun sequence.
ACCESSION   DAOMTA010000066 DAOMTA010000000
VERSION     DAOMTA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225_length_6169_cov_2.401210"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLK68_02900"
     CDS             complement(<1..303)
                     /locus_tag="PLK68_02900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM89391.1"
                     /translation="MKKILLVCCAGMSTSLLVNRMREVAKTKEIDCKIWSTSEKELPN
                     EFAQEPADVILVGPQIRYLLNNIKKEVNNTVPVELIDMRIYGMMDGEKVLNQALNAL"
     gene            complement(330..1724)
                     /locus_tag="PLK68_02905"
     CDS             complement(330..1724)
                     /locus_tag="PLK68_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89392.1"
                     /translation="MEDFLKDVYTSIYKKWILFQQIDNCQIMLSSKDQNKIILETKYG
                     VANVIFYKFNIIELNVISKIDQESCFFLHFQMNNINHAINLFYEMVECLKTLIKKPKI
                     KILLCCSGGLTTTYFAYKIDEAIQLFALDYEIAATGYNELFKKGEQYNVILLAPQVSF
                     MYAKVKKIFKDKYLLNIPAQVFAKYDVKEILNLVDQELIKKRNKNGQVQLLSIRNKTI
                     TFHRKILCISLFRNRNRIHIAYRLYQSQSDIIVNNETIKQRITIQDIYDVIDTVLLNY
                     PGIEVIGFSTPGIVNNGFATTASINGFDDMNYKKLFTSKYSQKFIITNDVNTAAIGYH
                     ATQNQYSSIVLLFQPMSTKAGAGIIIDNKLINGKHNVAGEMKYLPVNLLEKGANVYKT
                     PEDIIKIVKYISLSIISVIGPEAIVIFCSLLPNIEDLENELKTVLPQEYIPRLIKIDD
                     IQEYIFLGQTIICT"
     gene            1948..>2891
                     /locus_tag="PLK68_02910"
     CDS             1948..>2891
                     /locus_tag="PLK68_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89393.1"
                     /translation="MKNYIFKDFLKYTSLNVLGMIGLSCYILADTFFIANGVGEIGLS
                     ALNLALPMYCLINGLGLMIGIGGGTRYSLLKVNSNRTSQNIIFSNALYFMLILAIIFV
                     VLGLSASTSLANLLGANQDTTIYTATYLKTIMFFAPMFMLNNLLIGFVRNDDNPRLAM
                     MGMLIGSLTNVVLDYIFIFPFGLGMFGAALATGVAPVISISIMSPYVIKKANFKFLKT
                     KLCLNHCKDIFLLGLSALIGELATGIVMLLFNFTLLKYSGNIGVAAYGIIANIAFVIV
                     SMFTGVAQGIQPIISKNLSFKEYRNVNLAYHYGLWTVI"
     assembly_gap    2892..2901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2902..3294
                     /locus_tag="PLK68_02915"
     CDS             <2902..3294
                     /locus_tag="PLK68_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89394.1"
                     /translation="GRKRKKAIIIFAVIVYLSSYFAAAQIVALFNSSSNPELLDLAVR
                     GIHIYFGGFIFAGVNMISATFFSASDKPRQAFIISSLRGFILIAPVIFILSTIFKVDG
                     VWLTYVVTELITSIITIILKRKSDFSLH"
     gene            complement(3281..4627)
                     /locus_tag="PLK68_02920"
     CDS             complement(3281..4627)
                     /locus_tag="PLK68_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM89395.1"
                     /translation="MDAGPESSITLQLILIVVLTLINAYFAASEMAIVSVNKNKIRRL
                     SEEGNKKAKLVEKLLDQPTNFLSTIQVAITLAGFFNSASAATGISVSLANLLKVWSIP
                     YADTIAVVLITILISFITLIFGELVPKRIALQKAEWYSMFCAKPILIISKIAGPFIKI
                     LSWSTKFVLRLFGMDDENVEESLSREEIRSMVESGQETGVFNEIETDMITNIFEFDDS
                     LALNVMTPRTDVYCIDINDPLNENIDQMMSMQYTRIPVYDDSIDNIIGILNMKDFAIE
                     ARQVGFDNVDIRKLLRKPYFVLETKNIDDLFKELQEDRQHIAILVDEYGGFSGIVTVE
                     DLIEEIMGDIEDEYDHDDEPKLQKIDDYNFIVDGNYLIDDLDDELDLKLDNVNHDTIS
                     GFVLHLLGEIPDDNQERSVTYENLTFKITGVKGNRVTKIKLTIKKSEEKESDSSED"
     gene            4806..5612
                     /locus_tag="PLK68_02925"
     CDS             4806..5612
                     /locus_tag="PLK68_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89396.1"
                     /translation="MKNFDYKGSYINKTDHHIYIAPTEELKDIIAHYTITFPSEVPAS
                     SKLFHIIPDASGCFIFQGRSRRDFWGPMSEIVVLENDLQKAPARFFVEFRPGGLYQIS
                     GLHQKKLVNQRQQLRYLDVQLDEELALLYQSCKDYEQLIKAFNKYFTGKRKVNLLPAR
                     LIRAKEIIDQDHGNVSLEKVASDCHLSSRQLLRDFHNYIGLSGKEYAKVVRFNYLLKQ
                     IEEDDFLSLALQGGYFDQAHFNKVFKQITKTTPKKYLSNLADFYNEIYKF"
     gene            5642..5905
                     /locus_tag="PLK68_02930"
     CDS             5642..5905
                     /locus_tag="PLK68_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRM89397.1"
                     /translation="MIESRCGIICSECGFKSRIGCAGCINIEHPFWGEICPVKSCVET
                     KELNHCGECEDFPCKLAKSYAYDEKQGDQGKRLEQCRCWGKIK"
     gene            5902..>6169
                     /locus_tag="PLK68_02935"
     CDS             5902..>6169
                     /locus_tag="PLK68_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HRM89398.1"
                     /translation="MKLEATVLAVKNLEVSKQFYMQLFDQKIILDHGLNITFDGGFAL
                     QKDFAWLVDVPSDWVCDKACNMELYFEVDDFDKFIKKLETYPNIK"
BASE COUNT         2073 a          845 c          966 g         2275 t
ORIGIN      
        1 taatgcgttt aaagcttgat taagaacctt ttcaccatcc atcataccgt aaatacgcat
       61 atcaataagc tcaactggaa cagtattgtt tacttctttt ttaatattat taagtaaata
      121 tctgatttgt ggaccaacaa gaattacatc agcaggttct tgggcaaatt catttggtaa
      181 ttctttttca cttgttgacc agattttgca atcaatttct tttgttttag caacttctcg
      241 catccggttt actaataaac ttgtagacat tcctgcacaa catactaata aaatcttttt
      301 cataattttc tccttaattt ttatttaatt taggtacaaa taattgtttg acctaggaaa
      361 atatattctt gaatatcgtc aattttaatt aaccgaggaa tatattcttg tggtaatact
      421 gtttttaact cattttctaa atcttcaata ttaggtaaaa gactacaaaa gataacgatt
      481 gcctcaggcc caattacact aataatcgat aaagaaatat atttaacaat tttaataata
      541 tcttctggtg ttttatatac attagcacct ttctctaata gatttacggg gaggtatttc
      601 atttcacctg ccacgttatg cttgccattg attaatttat tatcaatgat gataccagca
      661 ccagctttag tactcattgg ctgaaaaagt aacacaattg atgagtattg attttgagta
      721 gcatgatagc caattgctgc ggtattaaca tcgtttgtaa taataaattt ttgtgaatat
      781 ttgcttgtaa acaatttttt ataattcata tcatcaaaac cattaatact ggcagttgta
      841 gcaaatccat tattaacaat tcctggtgtt gaaaaaccaa taacttcaat acctggataa
      901 tttaataaaa cagtatcgat aacatcatag atgtcttgaa ttgtaattcg ttgtttaatt
      961 gtttcattgt ttacaataat atcactttga ctttgataaa gtcgatatgc aatatgaatc
     1021 cgattacgat ttctaaaaag agaaatacat agaattttac gatgaaaggt gatggtttta
     1081 tttctaatcg aaagcaattg aacttgacca tttttgttcc gttttttaat tagttcttgg
     1141 tcaactagat taagaatttc ttttacatca tatttagcaa atacttgtgc aggaatatta
     1201 agtaaatatt tatctttaaa aatcttttta acttttgcat acataaaaga aacttgaggt
     1261 gctagtagga ttacattata ttgttcacct tttttgaata attcgttgta gccagttgct
     1321 gcaatttcat aatcaagagc aaataactga attgcttcat caatcttata agcaaaataa
     1381 gttgttgtaa gaccaccact gcagcataat aagattttta tttttggttt cttgatcaat
     1441 gtttttaaac attcaaccat ttcataaaat agattaattg catgatttat gttattcatt
     1501 tgaaagtgta aaaagaaaca agattcttga tctattttac taataacatt taattcaata
     1561 atattgaatt tataaaaaat aacattagct acaccatact ttgtctccaa gataatttta
     1621 ttttgatctt ttgatgaaag cataatttga caattatcga tctgttgaaa taaaatccac
     1681 tttttatata tcgatgtata tacatctttt aggaaatctt ccattttcac ctcccctttc
     1741 agtaataact gataactaat taaataataa tcaaaattat tggctaataa aatgaagatt
     1801 agtgttttag gctagtttat ttaaaagtta caacttgata aataaaaaag tgattaatct
     1861 agtcgcttta gttgaaatat ttttaaaatt tgattgacta taaggtaatc ttatagttta
     1921 agatgaagaa agactggagg aatgaggatg aaaaattata tttttaaaga tttcttaaaa
     1981 tatacatcgt taaatgtttt aggaatgatt ggtttatcat gctatattct ggcagatact
     2041 ttttttattg caaatggtgt tggtgaaatt ggattatcag ctctaaattt agctttacca
     2101 atgtactgtt taataaatgg cttaggacta atgattggaa tcggtggggg aacacggtat
     2161 tctttgttaa aggtaaatag taatagaacc tcacaaaata taattttcag taatgctctt
     2221 tattttatgc ttattctagc cattattttt gttgttttgg gactaagtgc gtcgacatca
     2281 ttagcaaatc ttttaggggc taatcaagat acaactatct atactgcaac ttatttaaaa
     2341 acaattatgt tttttgcacc aatgtttatg ttgaataatt tattgattgg ctttgttcgt
     2401 aatgatgata atccgagatt agcaatgatg ggaatgttaa ttggtagttt aactaatgtt
     2461 gttttggatt atatatttat tttccctttt ggtttaggaa tgtttggggc tgctttagca
     2521 acaggggtag caccggttat ttcaatttca attatgtctc catatgtaat caagaaagct
     2581 aattttaaat ttcttaaaac aaaattatgt cttaaccatt gtaaagatat ttttttatta
     2641 ggattatcag ctttaatagg agaattagct actggaatag taatgttgtt atttaatttt
     2701 actttgttga agtatagcgg gaatataggt gttgcagcct acggaattat tgctaatatc
     2761 gcttttgtta ttgtatcgat gtttacaggg gttgctcagg gaattcaacc aattattagt
     2821 aaaaatctta gttttaaaga atatcgaaat gtaaatttag cttatcatta tggtctttgg
     2881 acagttatta tnnnnnnnnn nggcagaaaa agaaagaaag ccattattat atttgcagtt
     2941 attgtttatc ttagcagtta ttttgctgct gctcagatcg tagctttgtt taattcaagt
     3001 agcaatccgg aattgctgga tttggcagtt agaggaattc atatttattt tgggggcttt
     3061 atttttgcag gagtaaatat gattagtgcc actttcttta gtgcaagcga taaaccacgt
     3121 caagctttta taatttcatc attacgaggt tttattttaa ttgcaccagt aatatttatt
     3181 ttatcaacta tttttaaagt agatggagtt tggctaactt atgtggtaac agaattgatt
     3241 acttctataa ttactattat tttaaaaaga aaatccgatt ttagtcttca ctagaatcgg
     3301 attctttttc ttctgatttt ttaattgtta atttgatttt tgttacacga ttacctttta
     3361 caccagtaat tttaaatgta agattttcat aagttacact acgttcttga ttatcatcag
     3421 gaatttctcc taataaatga agaacaaaac cactgattgt atcatgatta acattatcta
     3481 attttaaatc taattcatca tcaagatcgt caattagata atttccatca acgataaaat
     3541 tatagtcatc tattttttgt agtttaggtt catcgtcatg atcatactca tcttcgatat
     3601 ctcccatgat ttcttcgatt agatcttcaa ctgtgacgat ccctgaaaac ccaccatatt
     3661 catctacaag aattgcaata tgttggcgat cttcttgtag ttctttaaat aaatcatcaa
     3721 tatttttagt ctctaagaca aagtatggtt ttcgtaatag cttacgaata tcaacattgt
     3781 caaaaccaac ttgtctagct tcaatagcaa aatccttcat atttaaaatc ccaatgatat
     3841 tatcaattga atcatcataa acaggaattc gagtatactg cattgacatc atttgatcaa
     3901 tgttctcatt taatgggtca ttaatatcaa tacaataaac atcagttctt ggggtcatga
     3961 cgtttaaagc taaactatca tcaaattcaa aaatattggt aatcatgtca gtttcaattt
     4021 cattgaaaac tcctgtctct tgtccacttt caaccattga acgaatttct tcccgtgaca
     4081 aagattcttc aacattttca tcatccattc caaataatct taagacaaac ttagttgacc
     4141 aagataaaat tttaataaat ggtccagcaa ttttactgat gatcaaaata ggcttagcac
     4201 agaacattga ataccattcc gctttttgta aagcgattct ttttggaact aattccccaa
     4261 agattaaagt aataaaagaa attaaaatag taatcaagac aactgcgatt gtatcagcgt
     4321 aaggaattga ccaaactttt aaaagattag ctaaactaac agaaataccc gtagcagcac
     4381 tagctgagtt aaagaaacct gctaaagtaa tcgctacttg gatcgttgat aagaagttag
     4441 ttggctgatc taatagtttt tctactaact tagctttctt atttccttcc tcgcttaaac
     4501 gacgaatctt gtttttgttt accgaaacaa ttgccatttc gctagccgca aaatatgcat
     4561 tgattaaggt aagaacaacg attaaaatta attgcaatgt gattgaagac tcggggcctg
     4621 catccataaa ttaaaacact ccttttttat gtaattaaat tttatgagaa ttattctatc
     4681 aaattcatga ttgaatgtaa atatttatag ccaatgaaga ttgttaagat ttgattaaat
     4741 gtcctgccaa tattatactg agtatataaa ataatgcagg taatttaata taaagaggta
     4801 atttaatgaa gaatttcgat tataagggaa gttatattaa taaaacagat catcatattt
     4861 atattgctcc tactgaagaa ttaaaagata ttattgctca ttatacaatt acttttccta
     4921 gtgaagttcc cgcctcatca aaattatttc atattattcc tgatgctagt ggctgtttta
     4981 tttttcaggg acgctctcga agagattttt ggggtccaat gagtgaaatc gtggttttag
     5041 aaaatgattt gcaaaaagcc ccagctcgtt tctttgtaga atttagacca ggaggattgt
     5101 atcaaattag tggtttacat caaaaaaaac ttgtaaatca gcgtcagcaa ttgagatatt
     5161 tagatgtgca gcttgatgaa gagttagcat tactttatca atcatgtaaa gattatgaac
     5221 agttgattaa agcttttaat aaatatttta cgggaaaaag aaaagttaat ttattaccag
     5281 ctcgtttgat tagagctaaa gaaattattg atcaggatca cggaaatgtt agtttagaaa
     5341 aggtagctag tgattgtcac ctttcttcac gacaattatt acgtgatttt cataattaca
     5401 taggcttgag tggtaaggaa tatgctaagg tagtaagatt taattattta ttaaagcaaa
     5461 tagaagaaga tgatttttta tctttagcat tgcaaggagg ctattttgat caggctcatt
     5521 ttaacaaagt gtttaagcag attacaaaaa caactcctaa gaaatattta tcgaacttgg
     5581 cggattttta caatgaaata tataagttct aagttataat ttgcttagga tggtgatgaa
     5641 aatgattgaa tcgagatgtg gaattatatg tagtgaatgt gggtttaaaa gcaggattgg
     5701 gtgtgctgga tgtattaata tcgaacatcc tttttggggt gaaatttgtc cggttaaatc
     5761 gtgcgtagaa acaaaagaat taaatcattg tggtgaatgt gaggattttc catgcaaatt
     5821 ggctaaatcg tatgcttatg acgaaaaaca aggggatcag ggaaaacgtt tagaacaatg
     5881 cagatgttgg ggtaaaatca aatgaaatta gaagcaactg tcttagcagt caaaaatctc
     5941 gaagtttcta aacagttcta tatgcaattg ttcgatcaaa aaataatttt agatcatggt
     6001 ttaaatatta cttttgatgg tggttttgct ttacaaaaag attttgcctg gctcgttgat
     6061 gtccctagtg attgggtttg tgataaagct tgtaatatgg aattatattt tgaagttgat
     6121 gattttgata aatttataaa aaagttggag acatatccaa atattaaat
//
LOCUS       DAOMTA010000067         6150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_228_length_6150_cov_2.132404, whole genome shotgun sequence.
ACCESSION   DAOMTA010000067 DAOMTA010000000
VERSION     DAOMTA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228_length_6150_cov_2.132404"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PLK68_02940"
     CDS             complement(<1..1511)
                     /locus_tag="PLK68_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRM89399.1"
                     /translation="MKKRGKIVSLLVIVAMFFSYIVPIKAQGNWQQHLDQTILKVGYT
                     EVYKDNKPVVPNANLTITITGTGLSGKEYNTVLIDEEAREVEATKQNGVINGDTTQLV
                     LQVPGTLSGGQYQVRTTIDSESVTNGLILLNNVNLSSKISTNVTGYSGVTGDLIDGIQ
                     WAMYTPVTNKPTRENPGIFTFDYGEDLIDVYSLDLYSTYGNDQGPKVAEILYWDDSSQ
                     TWENILNNGSREFEFRWDKNIECEPFRIQFDQVYSTNKIMLKTLDSYTSWENTNDIVE
                     IQVWGYKSGELPSIQISSSQGNYGVKNIVPMVIKNKNKSTTINLELVDFKSHQSLARP
                     VKYQVEMKNGKLNYEFVIPKTVPVGTYSIHVTCEGLDLYSDEYRISLDQDINIENCSK
                     YLDISLVNDQAYKITSITDGDIKSGWSNNGNTSLLFEVKPENNKFANIALKKMRIYTS
                     NKNIETVTLKGIANTNYYRNVNVNENGMINDLAGDFKPDWKSDKNGDYFEVDPG"
     gene            1799..2512
                     /locus_tag="PLK68_02945"
     CDS             1799..2512
                     /locus_tag="PLK68_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM89400.1"
                     /translation="MRKYETISIDLKKRIENGEFEETKKLPTGGQLMKEYDASKNTIS
                     NAINLLVNDGLLFAIHGSGFYIRKPNKGTIKLNNTIGFSAEHPGEKLERDILSFKLID
                     CDTDLAEHLECKVGTPVYAIKRLMYIDDIPFAVEYTYYNKDIIPYLNEDIAKTSIFSF
                     IINDLKLTIGFSEKYIFAKPLSKEDQELLNLEPGAFGLIIDDNVFLSNGKKFNYSKIW
                     YNYKYANFFNNDKSTRKKS"
     gene            complement(2536..2733)
                     /locus_tag="PLK68_02950"
     CDS             complement(2536..2733)
                     /locus_tag="PLK68_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89401.1"
                     /translation="MSAGKYQIIETIMSYTSQYSFAELNKFSVQEMYNILAIFNQEEV
                     PEGCQPGHNQDSHHRNHNQPK"
     gene            complement(2809..>3595)
                     /locus_tag="PLK68_02955"
     CDS             complement(2809..>3595)
                     /locus_tag="PLK68_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89402.1"
                     /translation="MKNDCFSFIKFCNERIANSNDYNIARTIINHIEDIKILSLEKIA
                     EEANISPASVSRFINKAGFESFQAFKYEFELFTRDVKMRRIISHTQRFMRTTIENMSE
                     SLYVDGLANLRQTKLNLDIDKLKEIVKLLKTSKSVTFIGDSHELADFYTLQLEMLVND
                     IPAYLINFYEFEKMYLNQLDHNDTIVFIGVYKEWFSDAQKEILDYAKERKIKIIAFVQ
                     EEDYLKEYADLLYVYGIPDSYNDGYYSLPYLNRLLCEMIYFKL"
     assembly_gap    3596..3605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3758..4894
                     /locus_tag="PLK68_02960"
     CDS             3758..4894
                     /locus_tag="PLK68_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM89403.1"
                     /translation="MKNKLILLSILSVALVSSIRGSIIPGMNLLYQEYSAYPMSTVSL
                     IITIPSIAVIPTSYFCSKFVGTKVSYRTILIAMNAALLFGGTGPFFIDELYSRLFLRL
                     LFGIGIGIAISLNKSIILEYYTDNRQIKYLGYTTIISSLGAIFFQTLVGYLSKISTDF
                     MFLGYLPLVITLILSFYIPDIPLKAPSKSTIKKEKNIKLLTISIFFLILNMLMSSIGI
                     NLSTLFATKSFADIAVITVHANNINSICSISSGLLFNKIYNKFKNNFLPISLFLCALS
                     LAIYIFGKSYISMYITSGIFGFTYNTIILYIFLIAAQTAKEKGNAAVGGYMGIAAGIG
                     GFLPSFLVYLCQLFFNESIYSVLFIAIMFLVFVGFITIKFLPAK"
     gene            complement(4952..>6150)
                     /locus_tag="PLK68_02965"
     CDS             complement(4952..>6150)
                     /locus_tag="PLK68_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM89404.1"
                     /translation="FEHSREGRYWKSYDLITNVFASQALYELSALAKEFGDSDSAIKW
                     ADTSATLTKGINEYLTTEVDGVKIYAELYDIDNNMKFIKGMSWVNWAPMAAQWYAMDE
                     KIMQNTYDIYAKYDSQDYDDYKMLDVVYDFDTQQSGNHIIGKGLAWEIMFNRVKDDNE
                     KLDYLASFILDHSTPGGVYPETYQPNGNFSDVGNQEHASWQHYAMSCAYPELTKTYSL
                     QILEELINEIQVLDAKLYTLDSYAKLSRALDAAISGYNVENITKEQCDALYEALLAAK
                     NELTYLDADYQAVDAAIVAAEKLNKDDYKDFSKVEAAINAVVRGKNITQQAEVDAMAK
                     AINDAIDTLEKKEGSVTPINSIKPVDKKPVVPNTGDENLMSLYISLIGIMAGLFVIIK
                     KRKNIY"
BASE COUNT         2005 a         1096 c          756 g         2283 t
ORIGIN      
        1 ccgggatcaa cttcaaaata atccccattt ttatcacttt tccagtctgg tttaaaatca
       61 ccagctaaat cattgatcat cccattttca ttgacattaa cattacgata ataattagta
      121 ttcgcaatac cttttaaggt aacagtttca atgtttttat tactcgtata aattctcatt
      181 ttctttaatg caatattggc aaacttatta ttttcaggtt taacttcaaa caataaagac
      241 gtattcccat tattactcca accgcttttt atgtcaccat cagttatact tgtaatttta
      301 taggcttgat cattaaccaa agaaatatct aaatatttag aacagttttc aatatttata
      361 tcttgatcta acgaaatacg atactcatca ctataaagat ctaatccctc acaagtaaca
      421 tgaatactat atgtacccac tggtactgtc ttaggaatta caaattcata atttaattta
      481 ccatttttca tctcaacttg atatttgact ggtcttgcta gtgattgatg agatttaaaa
      541 tcgactaact ctaaatttat tgtagtagat ttattcttat tttttattac cattggtaca
      601 atatttttca caccataatt tccttgtgaa gaacttattt gaatagatgg taactctcca
      661 gatttataac cccatacctg tatttcaaca atatcattcg tattttccca actagtatat
      721 gaatctaatg tttttaacat gattttatta gttgaataaa cttgatcaaa ttgaattcga
      781 aatggctcac attcaatatt cttatcccac ctaaattcaa attcacgact gccattattt
      841 aaaatatttt cccaagtttg actactatca tcccaatata atatttctgc aactttaggg
      901 ccttggtcat ttccatatgt tgaatataga tctaaagaat acacatctat taaatcctct
      961 ccataatcaa atgtaaaaat tcctggattt tctctagttg gcttatttgt aacgggcgta
     1021 tacattgccc actgaatacc atcaattaaa tcgcctgtta ctcctgaata tccagtaacg
     1081 ttggtactga tcttactgct taaattaaca ttatttaata aaattagacc atttgtaaca
     1141 ctttcactat caatcgttgt tcttacttga tattgacccc ctgacaatgt tcctggtact
     1201 tgcagcacta attgagtagt gtcaccattg attaccccat tttgctttgt tgcttctact
     1261 tctcttgctt cttcatcaat cagtactgtg ttatattctt taccactcaa tcccgttcca
     1321 gttatcgtaa ttgtcagatt agcatttggt actaccggtt tattatcttt ataaacctct
     1381 gtataaccca ctttcaagat agtttgatct aaatgctgtt gccaatttcc ttgagccttg
     1441 attggtacaa tataactaaa aaacatcgcc actataacta ataacgaaac aatcttacct
     1501 ctctttttca taataaagtt tcctttccaa aataaacact atttcattcg tttatcccct
     1561 ttatttcccc aaactactaa cacatttatt atcaatattc acactccttt tctacatctt
     1621 ttgcgattgt taccaaacaa ttttattgtt atctactttt agaatacaac gttttaatac
     1681 cagtgtcaat acaaaatgta agggatttct tttttgtcct atatatgcaa aattgttacc
     1741 aaacacttta caaaataata attaaatgat atttttataa ataaggatgg gagatattat
     1801 gagaaagtac gaaacaattt ccattgattt aaagaaacga attgaaaatg gtgaatttga
     1861 agaaacaaaa aaactgccta ctggtgggca gctgatgaaa gaatatgatg ctagtaaaaa
     1921 caccatcagc aatgctatta atttgctggt taatgatgga ttgttatttg ctattcatgg
     1981 tagtggtttt tacattcgaa aaccaaataa aggaactatc aaattaaata acacaatcgg
     2041 tttttcagct gaacatcccg gtgaaaaatt agaacgtgat attttaagtt ttaaattaat
     2101 tgactgtgat actgatctag cagaacattt agaatgtaaa gttggtactc cagtttatgc
     2161 aataaaacga ttaatgtata ttgatgatat cccttttgct gttgaatata cttactataa
     2221 caaagacatc attccttatc ttaatgaaga cattgcaaaa acatcaattt tttcttttat
     2281 tattaatgat ttaaaactta cgatcggctt ttctgaaaaa tacatttttg ctaaaccact
     2341 atcaaaagaa gatcaagagc ttttaaacct agaacctggt gcctttggtt taattatcga
     2401 tgataacgtt ttcctttcaa acggcaaaaa atttaattac tcgaaaatct ggtataatta
     2461 taaatatgct aattttttca acaatgataa atcaactcgt aaaaaatcat aaacagtatt
     2521 atagtttatg attttttatt taggctgatt atgatttcta tgatgactat cttgattatg
     2581 ccctggctga cacccttcag gaacctcctc ttgattaaat atagctaaaa tattatacat
     2641 ttcttgaaca ctaaacttat taagttcagc aaaagaatat tgtgaagtat aagacataat
     2701 cgtttcaata atttgatatt tacccgcaga catatgatgt gatgaagcgt tatgatgagt
     2761 ttcttgatca acttgtgaac aatgatatcg attagttcca agcaggtttt ataatttgaa
     2821 ataaatcatt tcacataaca atcgatttaa atatggcaaa gaataataac catcattata
     2881 actgtcagga attccataaa catataataa atcagcatac tcctttaaat aatcttcttc
     2941 ttgaacaaag gcaatgattt ttattttacg ttcctttgca taatctaaaa tctctttttg
     3001 agcatcagaa aaccattcct tatatacacc aataaaaaca atagtatcat tatgatctaa
     3061 ctggtttaga tacatctttt caaactcata aaaattaatc aaatatgccg gaatatcgtt
     3121 gactaacatc tctaactgca gcgtataaaa atccgccagt tcatgactat caccaataaa
     3181 tgtaacactt tttgaagttt tgagtaactt aacaatttct tttaacttat caatatctaa
     3241 atttaattta gtctgtctta agttagcaag accatcaaca tataaagatt cactcatatt
     3301 ttcaatcgtc gtccgcataa atctttgagt atgagaaata attcggcgca ttttaacatc
     3361 cctagtaaac aactcaaatt catatttaaa agcctgaaag gattcaaaac cagctttatt
     3421 gatgaaacga ctaactgaag caggagaaat attagcttct tctgcaatct tctctaatga
     3481 taatatttta atatcctcaa tgtgattaat aatcgttcta gcaatattat aatcattact
     3541 atttgcgatt ctttcattac aaaatttaat aaaactaaaa caatcattct tcatcnnnnn
     3601 nnnnnttttt ccctccaaat tatctttata tttttataat acaacaaaat taaccttaaa
     3661 tatttaattt tatcttaata ttacaacttt ttcacacaat tattgcttta taatttaata
     3721 tttgctaatg ttaattaaaa ggtggtatca acatataatg aaaaacaaac tgatcttgtt
     3781 atcaatatta tctgtggcat tagtttcatc gattagaggc tcgataattc caggaatgaa
     3841 tttgttatat caagaatatt ctgcttatcc tatgtctact gtctctttaa tcatcacgat
     3901 tccctctata gctgttatcc ctacctcata tttttgtagt aagtttgttg gaacaaaagt
     3961 ttcttatcga actatattga ttgcgatgaa cgctgctctt ttatttggtg ggactggtcc
     4021 attctttatt gacgagctct attcaagatt atttttacga ctgctctttg gtattggaat
     4081 cggaattgcc atttctttaa ataaatccat cattttagaa tattacactg ataatcgtca
     4141 aatcaagtat ttaggttata caacgatcat ttccagtcta ggtgcaatct tcttccaaac
     4201 attagttggc tatttaagca agatttcaac tgactttatg tttcttggtt atttaccttt
     4261 agtaataaca cttattctat ctttttatat tcctgatatt cctttaaaag caccatccaa
     4321 atcaactatc aaaaaagaaa aaaacatcaa acttctcacc atcagtattt tctttctaat
     4381 cttaaatatg ctgatgtcat cgattggtat taatttgtca actttgtttg ctacgaaatc
     4441 atttgcagac atagctgtaa taacagtcca tgctaataac attaattcta tttgttcaat
     4501 cagttcgggt ctattattta ataagatata taataaattc aagaataatt tcctccccat
     4561 ttccttattt ctttgcgctt tatctttagc aatttacatt tttgggaaaa gttacatcag
     4621 catgtatatt acttcgggaa tcttcggctt tacctataat accattattt tatatatctt
     4681 cttgattgca gcgcagaccg ctaaagaaaa gggtaatgca gctgtgggcg gatatatggg
     4741 aatagctgct ggaattggtg gctttcttcc ctcatttcta gtttatcttt gtcaattatt
     4801 ctttaatgaa agtatttatt ctgttttgtt tattgctatc atgtttttag tttttgtcgg
     4861 attcatcact attaagtttt tacctgcaaa ataaaatcgt aacatataat tttaaatagg
     4921 tgcaaaagaa gaattcctag gaattcttct tttaataaat attctttctt ttctttatga
     4981 ttacaaacag acctgccatg atcccaatta aagagatata gagactcatt aaattctcat
     5041 cccccgtatt tgggactact ggctttttgt caactggctt aatcgagtta attggagtaa
     5101 cactgccctc tttcttttca agagtatcaa ttgcatcatt aattgctttt gccatagcat
     5161 caacttcagc ttgctgtgta atattttttc cacgaactac agcatttatt gctgcttcta
     5221 ctttggaaaa atctttataa tcatccttgt ttaatttttc agcagctaca atagccgcat
     5281 caacagcctg ataatcagca tctaaataag ttagttcatt ttttgctgct aaaagtgcct
     5341 cgtacaatgc atcgcattgt tctttagtaa tattttcaac attatagcca ctaattgctg
     5401 catctaatgc tcgactgagt ttcgcataag agtctagtgt atagagcttt gcatctaaca
     5461 cctgaatttc attgattaat tcttctaata tttgaagtga atatgttttt gttaattctg
     5521 gataagcaca gctcattgca taatgctgcc aagaagcatg ctcttggttt ccaacatctg
     5581 aaaaatttcc atttggttga tatgtttcag gataaacacc accaggagtg gaatgatcta
     5641 aaataaaact agctaaataa tctaactttt cattatcgtc ttttacgcga ttaaacataa
     5701 tttcccatgc taacccttta ccaataatat gatttccact ttgttgagta tcaaaatcat
     5761 agacaacatc taacatctta taatcatcat aatcttgact atcatatttt gcataaatat
     5821 cataagtatt ttgcatgatt ttttcatcca ttgcatacca ttgagctgcc attggagccc
     5881 aattgaccca tgacattcct ttaataaact tcatgttatt atcaatatca tataattcag
     5941 cataaatttt aactccatca acttcagttg ttaaatattc attgattccc tttgttaaag
     6001 ttgcggaagt atcagcccat ttaattgctg aatcactatc gccaaattct tttgctaaag
     6061 cacttagttc atacaaagct tgtgatgcaa atacatttgt aattaaatca taagatttcc
     6121 aatatcttcc ctcacgtgaa tgctcaaaac
//
LOCUS       DAOMTA010000068         6111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_232_length_6111_cov_2.640357, whole genome shotgun sequence.
ACCESSION   DAOMTA010000068 DAOMTA010000000
VERSION     DAOMTA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_6111_cov_2.640357"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLK68_02970"
     CDS             <1..444
                     /locus_tag="PLK68_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRM89405.1"
                     /translation="VVELAKVPNIVAMKDATDNIAYAMEVLAKTQGMDFDMYSGCDDN
                     ILPFICAGGKGVISVLSNIYPRQTELLAQLALKGDLPKAQELAYALEPVCRYLFIDVN
                     PIMPKAALARMNVCKPTLRLPLIETTEENKKLLFDAMDAFEKLGF"
     gene            446..1189
                     /gene="dapB"
                     /locus_tag="PLK68_02975"
     CDS             446..1189
                     /gene="dapB"
                     /locus_tag="PLK68_02975"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRM89406.1"
                     /translation="MKVLVYGYGLMGKKIAHKVRSQNDMELMGIVSYEFDEKVPEKTY
                     SSLKECDEKADVIIDFSHPNNLNDILTYALANQTKLVIATTGYSQEQLDQIKEASKEI
                     AIFQSYNTSFGVQMVTKMLRQFAKEFYDAGYDIEILEKHHNQKIDAPSGTAELLYEVM
                     AEEIDGVEACYDRSSRHEKRKKEEIGIQSLRGGTIFGEHTIMFAGVDEIIEIKHTALS
                     KEVFVQGAISAAYALNDKDNGLFTLKSLY"
     gene            1202..>1771
                     /locus_tag="PLK68_02980"
     CDS             1202..>1771
                     /locus_tag="PLK68_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRM89407.1"
                     /translation="MVLQTEFAQIKNNNLYIDDVKATELAKKYGTPLYVMSEGHIRHQ
                     FNKLKTKMIEKYENTLPLFASKSFSCIAIYKIAKEYGVGIDCVSAGEISVALKSGFDP
                     KKIYFHGNNKLPSEIEYALENGVENFVIDNFYEIDLVQEIASRLGVKINGIVRITPGV
                     YAGGHDYIRVGAKDTKFGFSLVFVFLANSL"
     assembly_gap    1772..1781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1782..2554
                     /locus_tag="PLK68_02985"
     CDS             <1782..2554
                     /locus_tag="PLK68_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRM89408.1"
                     /translation="SSHDNTYLKAIKKVIDAPNINFDGIHCHVGSQIEDIQAYVLAMN
                     KFVEIAYEIYDIFGIVINKLNAGGGFGIAYTAADNPLEFETVVDTIMEIITKGFDARG
                     LKRPMVLVEPGRYCVGNAGITLYTVGSSKYIKDIRDYITVDGGMTDNIRSSLYAAKYD
                     AIIANKAEMPTDHLITVAGKNCESGDILIKDIMLQDPEPGDILAMFSTGAYHYSMSSN
                     YNQLPKPAVVFTYEGKDREVIRRQTFDDLVYYDIDSKY"
     gene            2597..3001
                     /locus_tag="PLK68_02990"
     CDS             2597..3001
                     /locus_tag="PLK68_02990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM89409.1"
                     /translation="MELTYKNIKEISQERLVDLFKSVEWESANYPAQLVQAIKNYGSV
                     FSAWDNDKCVGLVASMDDSIMVAYVHYVLVNPQYQKYGIGKKLMQMMLEHYKDYHKVC
                     LIGVNTAVGFYEHLGFEVNEKAKPMFYLNKNY"
     gene            complement(3040..3870)
                     /locus_tag="PLK68_02995"
     CDS             complement(3040..3870)
                     /locus_tag="PLK68_02995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM89410.1"
                     /translation="MKDILKQLNTRPKLFEQSTASIWDDPHISKGMLKAHLNENQESA
                     TRKLDFVKKSVAWINTVLPNHHYNNLLDLGCGPGIYAELFYQYGYQVTGIDLSKRSIS
                     YAQASAKQKGFDIIYLRSDYTKSNFRQHYDLVTLIYCDFGVLPPATRKKLLQKIYNTL
                     SPKGALLFDVFTPLQYDGAVEHKNWQINENGFWHNDWHLVLNAFYRYDNVHTFLNQYT
                     VINEDRITTYNIWEHTFSLKELEKDLKNAGFTKLDFYKDVIGQKYDKTSKTICVIAQK
                     "
     gene            complement(4382..4924)
                     /locus_tag="PLK68_03000"
     CDS             complement(4382..4924)
                     /locus_tag="PLK68_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM89411.1"
                     /translation="MEYINIKGLDKPISKLIMGTAWFNPAFEEEIFTMLDQYVAAGGT
                     VIDTGRFYGANKDGEHACESERVLKKWFDSRNNRDQLVIMDKACHPIITPEGCHHPEY
                     WRVKPDIITDDLHYSLLHTGCDHFDIYLLHRDDPSVPVNEIMDRLEQHRQEGLITTYG
                     VSNWELDRVQAAVEYCQQMG"
     assembly_gap    5032..5041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5070..5657
                     /locus_tag="PLK68_03005"
     CDS             5070..5657
                     /locus_tag="PLK68_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM89412.1"
                     /translation="MELGIIHTLSSQYIPDTIRGYLQQYPQTGFKIQTDKTENIIKGL
                     KEHKYDIGFCGKIIAPELVFIPVLYQEFVAVVPPDHELANKQEISLEELVNYDLVGYL
                     DDLVIAQTVKEIFAEHKLEPRYISKQENELLIGGMINQGFGVGIAANTSFLKEFDLKL
                     KKDYRVIYLVYNKVDYISAAAENFINYIAINKINL"
     gene            complement(5683..>6111)
                     /locus_tag="PLK68_03010"
     CDS             complement(5683..>6111)
                     /locus_tag="PLK68_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM89413.1"
                     /translation="GTFSIRIPNPATGGYFHMGDSMIFLSVLILGKRDGAFAGALGGA
                     LADLLCGAAIWIGPTLIIKFIMAWIMGIIWEQYPSKIISAIGGGVFQIIAYTATETFL
                     FTWPAALGALPGLTMQTAGGIIIYVILARALQTTKLLNSD"
BASE COUNT         2111 a          883 c         1110 g         1987 t
ORIGIN      
        1 gtagtagaac ttgcaaaggt tcctaatatt gttgctatga aagatgcaac agataatatt
       61 gcatatgcaa tggaagtttt agctaaaact caaggtatgg attttgatat gtattcagga
      121 tgtgatgata atatcttgcc atttatttgt gccggaggaa aaggagttat ttcagtatta
      181 tcaaatattt atccacgcca aactgaattg ctggctcaat tagcattaaa aggtgattta
      241 cctaaagctc aagaattggc ctatgctctt gagccggtat gtcgctattt gttcattgat
      301 gttaatccaa ttatgcctaa agctgcatta gcaagaatga atgtatgtaa accgacattg
      361 agattgcctt tgattgaaac aaccgaagaa aataaaaaat tattgtttga tgcgatggat
      421 gcatttgaaa agttggggtt ttaagatgaa agtattagta tatggatatg gattaatggg
      481 gaaaaagatt gcccataaag taagaagtca aaatgatatg gaattaatgg gaattgtaag
      541 ttatgaattt gatgaaaaag ttcctgaaaa aacatatagt tcattaaaag agtgcgatga
      601 aaaagccgat gtcatcatcg acttttctca tccgaacaat ttaaatgata ttttaacgta
      661 tgcattagcg aatcaaacaa aattagtaat tgctacgaca ggttatagtc aagaacagct
      721 tgatcaaatt aaagaagcat ctaaagaaat tgctatcttt caatcatata acacatcatt
      781 tggggtgcag atggttacaa aaatgttacg acaattcgca aaagaatttt atgacgcagg
      841 atatgatatt gagatccttg aaaaacatca taatcagaaa attgatgcac cttcaggaac
      901 tgctgaatta ttatatgaag ttatggctga ggaaattgat ggcgtagaag cgtgttatga
      961 tcgcagcagt cgtcatgaaa aacgaaagaa agaagaaatt gggatccagt cattgcgtgg
     1021 gggaacaatt tttggggaac acacgattat gtttgcggga gtagatgaaa ttattgaaat
     1081 caagcatacg gctttatcaa aagaagtatt tgttcaaggt gcgattagtg ctgcttatgc
     1141 actaaatgat aaagataatg gtttatttac tttaaaatca ttatattaat agggggaaaa
     1201 tatggtctta caaacggaat ttgcacaaat taaaaataat aacttataca tagatgatgt
     1261 taaagcaaca gagttagcaa aaaaatatgg aacaccttta tatgttatgt ctgaaggaca
     1321 tattagacat caatttaata aattaaaaac gaaaatgatt gaaaagtatg aaaatacact
     1381 accattattt gcatcaaaat cattttcttg tattgcaatt tataaaattg ctaaagaata
     1441 tggtgttgga atcgattgtg tatcagcggg tgagatttca gttgctttaa aaagtggatt
     1501 tgatcctaaa aagatttatt tccatggaaa taataaatta ccatcagaaa tagaatacgc
     1561 gttagaaaac ggggtagaaa attttgtaat agataatttt tatgaaatag atttagtcca
     1621 agaaattgct tcgagattag gtgtcaaaat aaatgggatt gtcagaatca caccgggggt
     1681 ttatgcagga ggtcatgact atattcgagt tggtgccaaa gatacaaaat ttggtttttc
     1741 gcttgtattt gtttttttag ctaactcgct tnnnnnnnnn nttagcagcc atgataatac
     1801 atatctaaaa gcaatcaaaa aggtaattga tgctccaaat attaattttg atggaataca
     1861 ttgtcatgtt ggcagtcaaa ttgaagatat tcaagcgtat gttttagcaa tgaataaatt
     1921 tgttgaaatt gcttatgaaa tttatgatat ttttggaatt gtaattaata aattaaatgc
     1981 aggtggtggt tttggaattg cttatacagc tgctgataac ccattagaat ttgaaacagt
     2041 agttgatacg atcatggaaa tcattactaa aggatttgat gctcggggat taaaacgtcc
     2101 gatggtccta gttgagcctg gtcgttattg tgtaggaaat gctggaatca cattatatac
     2161 cgtcgggtca tctaaatata ttaaagatat tcgtgattat attacagttg atggtgggat
     2221 gactgataat attcgttcta gcttatatgc tgctaaatat gatgctatta ttgcaaataa
     2281 agcagaaatg ccgacggatc acctaattac tgttgctgga aagaactgtg aatctggaga
     2341 tattttaatt aaggatatta tgttacaaga cccagagccc ggagatattc tggcaatgtt
     2401 ttcaacagga gcgtatcatt attcaatgtc atcaaattat aatcaattgc caaaaccggc
     2461 agtggtattt acgtatgaag gtaaggatcg tgaagtgatt cgtcgccaga catttgatga
     2521 tttagtttat tacgacattg attcaaaata ttaattaaag cggcatatgc cgctttaaac
     2581 atagatggag gaatttatgg aattaaccta taagaatatt aaagagattt cgcaagagag
     2641 attagttgat ttatttaaat cggtagagtg ggagtcagcg aactatcctg ctcaattagt
     2701 acaggcaata aaaaactatg gaagtgtttt tagcgcttgg gataatgata agtgtgttgg
     2761 tctggttgct tcgatggacg atagtatcat ggttgcttat gttcattatg ttttagtcaa
     2821 tccacaatat caaaaatatg gaattggtaa aaaactgatg caaatgatgc tggaacatta
     2881 taaggactat cataaagttt gtttgattgg tgtaaatact gctgttggtt tttatgaaca
     2941 tcttggtttt gaagttaatg aaaaagcaaa accaatgttt tatctgaata agaattatta
     3001 ataaaaaatg actttttaag gagtcatttt tatttagtct tatttttggg caattacaca
     3061 aatagtttta cttgttttat catatttttg tccaatgaca tctttataaa aatcaagttt
     3121 agtaaagccg gcatttttta agtctttctc taattctttc agggaaaaag tatgttccca
     3181 gatattataa gtagttattc tatcttcatt aatgacagta tactgattta aaaatgtatg
     3241 gacattatca taacgataaa aagcatttaa aactaagtgc cagtcattat gccaaaaacc
     3301 attttcattt atttgccaat ttttatgttc taccgctcca tcatattgta aaggagtaaa
     3361 gacatcaaat aataaagcgc ctttggggga taatgtgtta tagatctttt gcaagagctt
     3421 tttcctagta gcaggaggaa ggacaccaaa atcacaataa attaaagtaa ctaaatcata
     3481 gtgctgtcta aagttcgatt tggtatagtc actacgaagg taaataatat caaatccttt
     3541 ttgtttggcg gaagcctgag cataactgat tgaccgtttt gatagatcga taccagtaac
     3601 ctgataacca tactgataga ataattcagc ataaataccg gggccacaac caagatcaag
     3661 taagttgtta taatgatgat taggtagtac agtattgatc caggcgactg atttttttac
     3721 aaagtcaagt ttacgggtag ctgattcttg gttctcgtta agatgagctt ttaacatgcc
     3781 ttttgaaata tgagggtcgt cccaaatact tgctgtactt tgttcaaata atttaggtcg
     3841 cgtatttaat tgttttaata tatctttcat cgtgttttgt cctttcgtat gatccggtaa
     3901 ctagataaaa taaaaaactg tggttatttc cacagttaag agttttgact attaatgtgg
     3961 aaattaaaat aatcatttgg gcataacaat aaaacctgtt tttgacaggt aaaaaattat
     4021 gcatttttga ttatctaagt tttccacgtt tcacttatgt tactgattat gtaacatcaa
     4081 gtgcgccgga caaaatcatc tccttaatag ttaccgtaat taaattatat agtatttgta
     4141 aagtaaaaac aattaataaa agccataact gctatggcat ttattaattt ataatttgtc
     4201 tgttttaaga catagataat caatttcagc aggagttaat cttattttta acgcctcagc
     4261 acatgaatca aattcacttt tattacgact gccaacaata gctgcaacat caaatggctg
     4321 gtcaagtaca tatgcaaggg caatattgat tgaaggaacc cctttgagtt ttcctaatcc
     4381 tttagcccat ttgttggcaa tattctactg ccgcttgaac acgatctagt tcccaattgg
     4441 atacaccata agtagtaatt aaaccttctt gacgatgctg ttctaaacga tccattattt
     4501 cattgacagg gacactagga tcatcgcgat gaagtaagta aatatcaaaa tgatcgcacc
     4561 ctgtatgaag taaactataa tgaagatcgt cagtaataat atctggcttt actcgccaat
     4621 actctggatg atggcagcct tcaggggtaa taattgggtg acatgcttta tccataatta
     4681 ctaattgatc acgattatta cgagaatcaa accatttttt taaaactcgt tcagattcac
     4741 aagcatgttc tccatctttg tttgccccat aaaatctacc tgtgtcgatt actgttcctc
     4801 cagcagcaac atattgatcc agcattgtaa aaatttcttc ttcaaaggct ggattaaacc
     4861 aggctgtacc cataattaat tttgaaatcg gtttgtctaa acctttaatg ttaatatatt
     4921 ccattttttc atctcctaag cagttttttt gattaaataa gtatttaaga taagaccgat
     4981 gattgcaaca ccaatagcaa ctaaatagac attgttatag gcaagggggc cnnnnnnnnn
     5041 nagctatctg aggtccaaat agagagccga ttgaattagg aataattcat acactatcat
     5101 cacaatatat tccagataca atcagaggtt atctccagca atatccccaa acagggttta
     5161 aaattcaaac tgataaaaca gaaaatatta ttaaagggct aaaagaacat aaatatgata
     5221 taggtttttg tgggaaaata attgctccag aattagtttt tattcctgtt ttatatcagg
     5281 aatttgttgc tgttgttcca ccagatcacg aacttgcaaa taagcaggag ataagtttag
     5341 aagaattagt aaactatgat ttagtaggct atcttgatga tttagtaatt gctcaaacag
     5401 ttaaagaaat ttttgcagaa cataaattgg agccacgcta tatttctaaa caggaaaatg
     5461 aattattgat tggtgggatg atcaatcaag ggtttggtgt aggaatcgct gctaatacat
     5521 catttttaaa agaatttgat ttaaaattaa aaaaagatta ccgtgtaatc tatctagttt
     5581 ataataaagt agattatatt tcagcagctg cagaaaactt tatcaactat attgcaatta
     5641 ataaaataaa tttataaaaa attgctgaaa tgcagtaact ctttaatcac tgtttaataa
     5701 ttttgttgtc tgtaaagctc ttgctaagat aacataaata ataatgccac cagcagtctg
     5761 catagttaga ccaggtaaag ctccaagtgc agcgggccaa gtaaataaaa aagtttcagt
     5821 tgctgtataa gctataattt ggaaaacgcc accaccaatt gcacttatta ttttacttgg
     5881 atactgttcc caaataattc ccatgatcca ggccataatg aatttaataa ttaaagttgg
     5941 tccgatccag atagctgcac cacatagtaa atcggctaaa gcacctccta atgcaccagc
     6001 aaaggcacca tctcttttac ctagaatcaa tacacttaaa aagatcatac tgtcgcccat
     6061 atgaaagtat cctccagtag ctggattggg aattctaata ctgaatgttc c
//
LOCUS       DAOMTA010000069         6104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_234_length_6104_cov_2.152587, whole genome shotgun sequence.
ACCESSION   DAOMTA010000069 DAOMTA010000000
VERSION     DAOMTA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234_length_6104_cov_2.152587"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..2365
                     /locus_tag="PLK68_03015"
     CDS             128..2365
                     /locus_tag="PLK68_03015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRM89414.1"
                     /translation="MNENLKKRIIVSLATVSAFSSLAMVPLSAYGEYQGNDGTQKLNN
                     GLTSTSDYNDWYNNQWNNQESGEMDTGKIVLTPGAKATDLNFAWYSEEMGTPTVKIST
                     NQDMSGAKTVTGSADKINKNNSFKNYTASNKVALKDYLVENMTYYYQYSTNGVDWSDT
                     YTYKTHSFSDYQAVLVGDPQIGASGSNGQGTQDDTDIAVNTYAWNKTLQKALGAGGIA
                     ENASFILSAGDQIDYSSSGTNGSGEIIREQEYAGFLYPDVLRSTPLATTIGNHESMVD
                     DYSLHYNNPNASTLGSTESGGDYYYSYGDTLFISLNSNSRNVEEHRQLMKEAVASHED
                     AKWKVVLFHHDIYGSGSPHSDVDGANLRILFAPLMDEFNIDLCLTGHDHSYARTYQIL
                     DGKVIETDGVSENASKAYNPEGTLYIAAGSASGSKFYTLNTVKQYYIAERSNTPEPTF
                     STIDFSGDSLTIKTYDYNGQKYANDVTLSKDGNAKSIEEMKNEVAAIDTVNVTSGSKN
                     RIDEALIAVNTALDTRDDSTAETELQNKWNTTSDPLNYYGYAQNGFANENSTALKRGY
                     SSLLDKTLYENDSNKAVTTATIDEAYNKLATAKNEVVTKAEFAEVQTKFDQIGSTLAQ
                     ISIGDKKDQYTRADVDAFKKSIAALKVDFNEATITKTALNELSTQLDTVTNEFLAKKN
                     TEDITTAPIVTPSTTPSKTPVKTTSSKVKTGDDTSINLAGITAFVSLLGIAGTKLFKK
                     RKIEE"
     gene            2432..3601
                     /locus_tag="PLK68_03020"
     CDS             2432..3601
                     /locus_tag="PLK68_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM89415.1"
                     /translation="MKELLKEFKQLSKKEKIGHFGVWLAILAFLIFLYFFNNAVEKTP
                     LMDQEGAVFEKARVTEIISENRTENGLQQGTQIVNTEILSGDYKGQIVETTNIDSYLY
                     GADCKVGTRVIVQLSEYNGTLSASVYNYDRTNTLYTMVAIFLILLVVIGKRKGFTSAL
                     GLIFTFICIIFLYLPMLYLGFSPFFSAVAVVVLTTLVTMYFIGGFSMKTLCSVLGTIA
                     GVVVAGIFASSFGALGHVSGYNVNDIETLLYIGQNSKLDISGLLFSGILIASLGAVMD
                     VAMSISTTIEEIKYHNPSISRKDLFKSGIKIGGDMMGTMSNTLILAFTGGSLSTLVVF
                     YAYDMPFLQMFNSYDMGIEIIQGIAGSLGVILTVPFVSIIAAILMTKKKKGDFPK"
     gene            3658..4446
                     /locus_tag="PLK68_03025"
     CDS             3658..4446
                     /locus_tag="PLK68_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89416.1"
                     /translation="MTKVLFFDIDGTLTDTHGGLPEIPAGAQRKMKELQERGYLLFLA
                     SGRPYAFIPEILTNFGFDGAVLCNGAHVEMNGQFIYHQPTDLKKTIDLVNHLDENNFE
                     YIIETKRGAYLDPSFKVLEKFFISCNINEEFLIEDFNREDIIAECLKLEVSVPDEHQA
                     KIEEIILNDFNYDKHGTDNAFEIYSNVISKATGVQKVLEYLNLNVQDSYAFGDGLNDL
                     EMIQTVGTGVAMGNAVDELKAVSDLVCDSVHNNGLEKVLNDLFG"
     gene            complement(4490..5638)
                     /locus_tag="PLK68_03030"
     CDS             complement(4490..5638)
                     /locus_tag="PLK68_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HRM89417.1"
                     /translation="MHKLGISVYPDKSPKEEVYAYMEKAAKLGFSRIFTCFLSIPEDK
                     RESYLVEFKEFMDKAHELGFEVAADTNPEVFKIIGATPGNLKPFADLGLDIIRLDGNF
                     GTQGDIQITRNPYGIKIEFNASMDMGVELLINHGGNKDQMIMCHNFFPERYSGLDFNL
                     FMEYNRYWKELNLHTAAFVSSNNANTIGPWEVFCGLPTVEIMRGLPIDLQARTLLATG
                     DVDDILIGNYPATDEELEALSKINYQAIEIRVDEVPEITDNEKLIMYDFAPHWDRYDH
                     SSFMLRSSMPRVKFKEKATVQDSGFTSNTEIAGSKSIPHHDCGKKVFTRGDVLIVNDN
                     LAHYRGELEIVLTEIPNDGERNLVATVKEEEMILLDFVTPGHHFVLKK"
     gene            5743..>6104
                     /locus_tag="PLK68_03035"
     CDS             5743..>6104
                     /locus_tag="PLK68_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRM89418.1"
                     /translation="MNPSLIMSFVVTMIVSAILIPLVMKAGKELGIVAHKNKRTVHKV
                     EVPRIGGYAIYISSLIGAVIFLKTDPQINAILIAGFLVFFVGLIDDVHDLSPKTKLAV
                     ELIAALIVIVYGDIYLKG"
BASE COUNT         2105 a          844 c         1147 g         2008 t
ORIGIN      
        1 taatcgtctt ttttatggtt ttggttaagt tgataaagtg tttgtaaact tcgttgacgc
       61 taaaatctca gtgtgtaaaa taggtggcgt agtacagatt tataacttaa aaaaagggga
      121 ctatgacatg aatgagaatt tgaaaaaacg tattattgtt tctttggcaa cagtatcagc
      181 cttttctagt ttggcaatgg taccattatc tgcttatggt gaatatcaag gtaatgatgg
      241 aacacaaaaa ctaaataatg ggctgactag tactagtgat tataatgatt ggtataataa
      301 ccaatggaat aatcaagaat ccggtgaaat ggatactgga aaaattgtat tgacaccggg
      361 agcaaaagca actgatctaa attttgcctg gtactcagaa gaaatgggaa caccaacagt
      421 aaaaatttct accaatcaag atatgtcagg agcaaaaact gtaacgggca gtgctgacaa
      481 gattaacaag aacaatagtt tcaaaaacta tactgctagt aataaggtag cacttaaaga
      541 ttatctagtg gaaaatatga cttactatta ccaatatagt acaaacggtg ttgattggag
      601 tgatacatat acttacaaaa cacacagttt tagtgattat caggcagtat tagtaggaga
      661 tccacaaatt ggagcttctg gaagtaacgg acaaggtact caagatgata ctgatattgc
      721 agttaatacc tatgcatgga ataagacatt gcaaaaagca ttaggagctg gaggcattgc
      781 agaaaatgct agcttcattt tatcagcagg agatcaaatt gattattcat caagcggaac
      841 taatggttct ggagagatta taagagaaca agaatatgca ggtttcttat atcctgatgt
      901 gttgagaagt acaccattag caacaacgat tggtaatcat gagtcaatgg tcgatgatta
      961 cagtcttcat tataataatc ctaatgcaag tacattaggt tctacagaat ctggtggaga
     1021 ttattattat agctatggag atactctatt tattagttta aatagtaata gtcgtaatgt
     1081 tgaagaacat cgtcagttaa tgaaagaagc tgtagctagt catgaagatg caaagtggaa
     1141 agtggtttta ttccatcatg atatctatgg ttctggttca ccgcattcag atgttgatgg
     1201 ggctaactta agaattttat ttgcgccatt aatggatgaa tttaacattg atttatgttt
     1261 aacaggtcac gatcattctt acgctcgtac ttatcaaatt ctagatggaa aggtaattga
     1321 aaccgacggt gttagtgaaa atgctagtaa agcatataat ccagaaggaa cattatatat
     1381 tgccgcagga tctgcttcag gaagtaaatt ttacacattg aatacagtta aacaatatta
     1441 tattgctgaa cgttctaata caccagaacc aaccttttca acaattgatt ttagtggaga
     1501 ttctttaaca attaaaactt atgattacaa tggtcaaaaa tatgcaaatg atgtaacttt
     1561 atcaaaagat ggtaatgcta aatcaattga agaaatgaag aatgaagtag ctgcgattga
     1621 tacagtcaat gtaacaagtg gctctaaaaa tagaatcgat gaagctttaa tagctgtaaa
     1681 tactgcttta gacacacgtg atgatagtac agctgaaaca gaattacaaa ataaatggaa
     1741 tactacaagt gatccattaa attattacgg ttatgcccaa aatggttttg caaatgaaaa
     1801 tagtactgcc ttaaaacgag ggtattcatc tttattagac aaaacgttat atgaaaatga
     1861 tagtaataaa gctgttacaa ctgcaactat cgatgaagca tataataaat tagcaacagc
     1921 taaaaacgaa gtcgtgacaa aagctgagtt tgctgaagta cagactaaat ttgatcaaat
     1981 tggaagtact ttagcacaaa ttagtatcgg agataaaaaa gatcagtata ctagagcaga
     2041 tgtagatgct ttcaagaaat caattgcagc tttaaaagtt gacttcaatg aagcaactat
     2101 tactaagact gcattaaatg aattgagtac acaattagat acagtgacaa atgaattttt
     2161 agcaaagaaa aatactgagg atattacaac agctccaatt gttactcctt caacaacgcc
     2221 aagtaaaaca ccagtaaaaa caactagcag caaagtaaaa actggtgatg atacgtcaat
     2281 caatttagca ggtattactg ctttcgtatc attgctagga attgctggaa caaaattatt
     2341 taaaaaaaga aaaattgaag aatagtcaag ataaataatg aaataaacaa gacagaactt
     2401 gttctgtctt gttttatata tgggagaagt tatgaaagag ttattaaaag aatttaaaca
     2461 gttaagtaaa aaagaaaaaa taggtcattt tggagtctgg ctggcaattt tagcattttt
     2521 aattttttta tattttttta ataatgctgt tgaaaaaaca ccattgatgg atcaagaagg
     2581 tgctgtattt gaaaaagccc gagtgactga aattatatct gaaaatcgta cagaaaatgg
     2641 acttcagcaa ggaacgcaaa tagtgaatac ggagattttg tcaggtgatt ataaaggtca
     2701 aatagtagag actacgaata ttgatagtta tttgtacggt gccgattgta aggtgggaac
     2761 tagggtgata gtgcagctta gtgaatataa tggaacctta agtgctagtg tttataatta
     2821 tgatcgcact aatacactat atacaatggt cgcaattttt ttgattttat tagttgtaat
     2881 cggtaaacga aaaggtttta cctcagcttt aggattaatt tttacattta tttgtattat
     2941 ctttttatat ctgccgatgt tgtatttagg tttttcaccg ttttttagtg cagttgctgt
     3001 agttgtttta acgactcttg taacaatgta ttttataggt ggcttttcaa tgaaaacttt
     3061 atgttcggta ctaggaacga ttgcgggggt cgttgtagca ggaatctttg caagcagttt
     3121 tggggcactt gggcatgtta gcggctataa tgttaatgat attgaaacgt tactttatat
     3181 tggacaaaac agtaaattag atattagtgg tcttttattt tcaggtattt taattgcttc
     3241 gcttggagca gtaatggatg tagctatgtc aatttcaact acgattgagg aaattaaata
     3301 tcacaatcct agtatctcac gtaaagattt atttaaaagt ggtattaaaa taggtggaga
     3361 catgatggga acgatgtcaa atactttaat tttagctttt acaggtggct ctttaagtac
     3421 attagttgtt ttttatgctt atgatatgcc gtttttacaa atgttcaatt cctatgatat
     3481 gggaattgag atcattcaag gtattgcagg gtcattggga gtcattctaa cagttccatt
     3541 tgtttcgata attgctgcga ttttaatgac aaaaaagaaa aaaggagatt ttccaaaata
     3601 gtaaaaagcc gctagttatc tggcggtttt gctataataa aaaaatgagg tggatatatg
     3661 acaaaagtac tattttttga tattgatgga acattaacag atacgcatgg gggattgcca
     3721 gaaattccag ccggagccca acgaaaaatg aaggaattac aagaacgagg ttatttgtta
     3781 tttttagcaa gtggtcgtcc ttatgctttt attccagaga ttttaacaaa ctttggattt
     3841 gatggtgctg ttttgtgtaa cggagcacat gtggaaatga atggacaatt tatttatcat
     3901 cagcctacag atttaaaaaa gacgattgat ttagtaaatc atctggatga aaataatttt
     3961 gaatatatta ttgaaacaaa acgtggagca tatttagatc cgagttttaa agttttagaa
     4021 aagtttttca taagttgtaa tattaatgaa gaatttttaa ttgaagactt taatagagaa
     4081 gatataattg cagaatgctt aaaattagag gttagtgtac ccgatgagca tcaagcaaag
     4141 attgaagaaa ttatcttaaa tgattttaac tatgataaac atggaacaga taatgctttt
     4201 gaaatctatt ctaatgttat ttcaaaagct actggggtac aaaaagtcct tgaatattta
     4261 aatttgaatg ttcaagatag ttatgctttt ggcgacggtt taaatgattt agagatgatt
     4321 caaacagttg gaacaggagt tgcaatggga aatgctgttg atgaattaaa agctgttagc
     4381 gatttggttt gtgattcggt tcataataat ggacttgaaa aagttttaaa tgatttattt
     4441 ggataaaaaa accaagaatt tcaaatgaaa ttctcggttt tattttttac tattttttta
     4501 aaacgaagtg atgaccaggt gttacaaaat ctaataatat catttcttct tctttaactg
     4561 ttgctactaa attacgttca ccatcatttg gaatttctgt taaaacaatt tccaattcac
     4621 cacgataatg agctaagtta tcattaacaa tcaagacatc accacgtgtg aacacttttt
     4681 taccacaatc atgatgaggg attgatttac tgcctgcgat ttctgtattt gaagtaaatc
     4741 cagaatcttg aactgtagct ttttctttaa atttaactct tggcattgaa gaacgtaaca
     4801 tgaatgatga gtgatcataa cgatcccaat gaggggcaaa atcatacatg atcaattttt
     4861 cattgtcagt aatttcaggt acttcatcaa cacgaatttc aattgcttga taattaattt
     4921 ttgataaagc ttctaactct tcatcagtag ccggatagtt tccgattaag atatcgtcaa
     4981 cgtcaccagt tgctaataat gtacgagctt gtaagtcgat tggtaaacct ctcatgattt
     5041 cgactgtagg taaaccacag aatacttccc atggtccaat tgtattagcg ttgttgctag
     5101 aaacaaaagc agctgtatgt aagtttaatt ctttccaata acggttatat tccatgaata
     5161 gattgaaatc taatcctgaa taacgttcag ggaagaagtt gtggcacatg atcatttgat
     5221 ctttatttcc accatgatta attagtaatt caacacccat gtccatgctg gcattgaatt
     5281 caattttaat accatatgga ttacgtgtga tctggatatc accttgtgtc ccaaagtttc
     5341 cgtctaaacg gatgatgtct aatcctaaat cagcaaatgg ttttaaattt cctggtgtag
     5401 ctccaataat tttaaatact tcagggttag tatcagctgc tacttcgaac cctagttcat
     5461 gagccttatc catgaattct ttaaattcta ctaaataact ttcgcgctta tcttcaggga
     5521 ttgataagaa acaagtaaag attctactaa accctaattt agcggctttt tccatataag
     5581 cgtatacctc ttcttttggt gatttatcag ggtatactga tattcctaat ttatgcattt
     5641 cgttttcctc cttaacacca ttattataaa actatttatt ggttttgtct tgtattttat
     5701 gcaaaaataa ttgataatat acaaaagata ggaagtgaaa agatgaatcc tagtttgatt
     5761 atgtcatttg ttgtaactat gatcgtttca gccattctaa ttccgctagt tatgaaggcg
     5821 ggtaaagaat taggaatcgt agcacacaaa aataagcgaa cagttcataa ggtcgaagta
     5881 cctcgaatcg gtggatatgc aatttacatc agttccttaa ttggggcggt catcttttta
     5941 aagactgatc ctcaaatcaa tgcaattcta attgctggtt tcttggtatt ctttgtaggt
     6001 ttgattgatg atgttcatga tttatcaccg aaaacgaaat tagcagttga attgattgca
     6061 gcactgattg ttattgtata tggtgatata tatttaaaag gttt
//
LOCUS       DAOMTA010000070         6093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_235_length_6093_cov_2.635144, whole genome shotgun sequence.
ACCESSION   DAOMTA010000070 DAOMTA010000000
VERSION     DAOMTA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235_length_6093_cov_2.635144"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..835)
                     /locus_tag="PLK68_03040"
     CDS             complement(101..835)
                     /locus_tag="PLK68_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM89419.1"
                     /translation="MSDNIITIKHLKKHYQALPVLLDINLEVNRGDVISIIGASGSGK
                     STFLRCLNLLEIPDHGEILYCNKNILKEELNLNKYRSDVGMIFQSFNLFNNLTVLENC
                     VIGQMKVLNRNREEAEEKALLYLQKVGMAEFAKKSSTQLSGGQKQRVAIARALCMEPQ
                     VLLFDEPTSALDPQMVGDILKIIKHLALKGMTMIIVTHEMQFARDVSNKVIFMHNGII
                     EEAGTPEQIFEHPRSMGMIQFLSRQY"
     gene            complement(852..1556)
                     /locus_tag="PLK68_03045"
     CDS             complement(852..1556)
                     /locus_tag="PLK68_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM89420.1"
                     /translation="MFDFDKSFTIFLDNFDLFWYGTKITIILALVGTIAGLIIGLLFG
                     AIRATTIDIKDKKIIVLIKKALKIISEIYIWFFRGTPMLVQAMFFYHGLRPFFQWNAL
                     TAGIMIISINTGAYMAEIVRSGIQSVDRGQSEGALSLGMTRVQTMKYIILPQAIRNSF
                     PAIGNQFIINIKDSSMLNVIGVVELFFQSSSIAGSTMSYSATFLITCLVYLCLTSIAT
                     ILLNIIEKRINNPILR"
     gene            complement(1559..>2030)
                     /locus_tag="PLK68_03050"
     CDS             complement(1559..>2030)
                     /locus_tag="PLK68_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM89421.1"
                     /translation="IDFTTPYYQSEMVMIVRGDDASKNYTDIQQFSGKTIVGQMSTSY
                     DTVIDQINGVDHATPKQSYPEMLVALQSGEVDGITAELPVAQGILETNQDLAIVRFEQ
                     NKGFKVDTAVSIGLKEGSRNSTLFKKVQKCIDNISSEKRNEKMGKYSSSQPKGE"
     assembly_gap    2031..2056
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2057..2406)
                     /locus_tag="PLK68_03055"
     CDS             complement(<2057..2406)
                     /locus_tag="PLK68_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM89422.1"
                     /translation="MKKILAFLLIALLISGCESNSGNKSIDEGDVFVVGMECNYPPFN
                     WQTNTQSDTAVELEGSGYADGYDVYIAKEIAESLDKKLVIKKLAWNGLQPALESGEID
                     AIIAGMTADEEREKG"
     gene            complement(2745..>6093)
                     /locus_tag="PLK68_03060"
     CDS             complement(2745..>6093)
                     /locus_tag="PLK68_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HRM89423.1"
                     /translation="QDTYSEVAGESHTGSFSVDSLTLQPGATTTSINLNWYAPAGTTV
                     AKIQFGDQTYDVTAKPLTSPTEIKSDKYTDTGKLVCKTTISNLKPDTKYTYYISNDGG
                     TTWSKEYNYTTPSSNEFTFGFTSDPQIKENKEINSEGWNPSDETNQTGWATMMETLEK
                     QGVDLVVSAGDQVEDQNWGKSSEYEAFFAPEEMASIPYAPAVGNHDRHYMFADHFNLP
                     NQMESLTEVKTTFRGQNSGTSQSHGNYIQATENEIKNNAASNGVTPNSDGQYDFSERR
                     DMETQGNYYYLYNNVLFVTLNTSAYPGGNDEENANNPNVSSASRDNSEAEAIVNNFAQ
                     TLKSATSEYQNQYQWIIVTHHKSTQTVAKHAADSDIENYVDAGFEKLMDEYNVDFVLG
                     GHDHVYSRSYVLKDGQRNSERLDTLNNPQGTIYITGNCCSDMQYYTPFETLDKNNNAD
                     YPILANGKSGSAAYLEGDSLPVGNQEWNQEYSPSYAVFNVENNRISVKVYNLAGDQTN
                     PDSKEIDAFTVTKNSDGGEKTTGYENNSALLALEQSARYDSGTTNSDGGVTEIVDYNT
                     VTGWAYAVNGQSGNLTAITLKNIENKDKIDLLDGNDIDISSLVQSADFTYGDMTSVAV
                     SHDGTSLAVAIQGKETNANGRVALFDCNQDGTITLKQLFEVGVQPDMVVFTNDDGQIL
                     TANEGEPRDGYTGAVDPKGSISVINTKTNKAKTVDFTAFDSQRDALIEKGIVLKKGVL
                     PSTDLEPEYIAVSDSKAYITLQEANAIAKLDLNSLEIEDIYSVGFEDYSKIAIDIDKK
                     DEKYNAQTYDSLKGIRMPDAISVYTVDGIDYLITANEGDSRDWNGYSNEIEVNFGKGK
                     TSPTGKITADNSGLTGKVTFFDTSDYDGLNNENDYLFGGRSSTIFKADEQSLQEIYTT
                     GNDFEVKTAAYLPNNFNCSNDDATIDDRSGKKGPEAEAVTIGQIEDKTFAFIGLERIG
                     GIMVYDITNPEKTEFVNYINSRDFSTDIGGDDSPEGIHFIAGNDSITGKPQLVVAYEV
                     SGTVGVYDLTLQKNSDQSGDDKTDNPSQEPTVEETNNSSNTNTNNKNHSVNTGDASNI
                     AITIISLISTIVLITILMKKDKVLAED"
BASE COUNT         1884 a         1154 c          772 g         2257 t
ORIGIN      
        1 gatgagttta gcatgaataa aattaatgta gcgttaagtt aaatattaaa gatattaaga
       61 tagtattaat atctattaaa aagagaacta attaagttct ctaatattgc cgtgataaaa
      121 attgaatcat tcccatactt cgaggatgct caaatatttg ctctggtgtt cctgcttctt
      181 caataattcc attatgcata aatataactt tattcgaaac atcacgcgca aactgcattt
      241 catgagtcac aataatcata gtcatacctt ttaacgctaa atgtttaatg atttttaaga
      301 tatctcctac catctgagga tctagtgcac ttgttggttc atcaaataaa agtacctgtg
      361 gttccataca taaagctctt gcaattgcaa cccgctgttt ttggccccca gaaagttgtg
      421 tactgctctt tttcgcaaac tcagccatac caactttttg tagatataat aaagcttttt
      481 cttcagcttc ttcacgattg cgatttagta ctttcatttg ccctataaca caattttcta
      541 aaactgtaag attattaaat aaattaaagc tttgaaaaat cattccaaca tcactacgat
      601 atttattaag attaagttct tcttttaaaa tatttttatt acaatacaat atctcaccat
      661 gatcaggaat ctctaataaa ttaagacatc ttaaaaaagt tgatttacca ctgccacttg
      721 ctccaatgat actaattaca tcacctcgat taacctctaa attaatatct agaagtaccg
      781 gcagggcttg ataatgtttt tttaaatgtt taatagtaat aatattatca ctcataaata
      841 atttcctcac tttatcttaa tattggatta tttattcttt tttcaattat atttaataaa
      901 attgtcgcaa ttgaggttaa gcacaaataa accaggcatg ttattaagaa ggtcgctgaa
      961 tatgacattg ttgatccagc aatactgcta gattggaaaa ataattctac aaccccaatc
     1021 acatttagca ttgaactatc tttaatatta ataatgaatt gattcccaat tgcgggaaat
     1081 gaatttctaa ttgcttgcgg taatataata tacttcatag tttgaaccct tgtcattcct
     1141 aatgataacg ctccttcact ttgcccacga tcaacagatt gaattcctga acgaactatt
     1201 tctgccatgt atgctccagt attaatagaa atgatcataa ttccggcagt aagtgcattc
     1261 cattgaaaaa aaggtcgcaa tccatgataa aaaaacatag cttgaaccag cattggagtt
     1321 cctctaaaaa accaaatata aatttctgaa attattttta atgctttttt tattagtaca
     1381 ataatctttt tatctttaat atctatcgtt gttgcacgaa tcgctccaaa tagcagtcca
     1441 attattaaac ccgctattgt tccaactaat gccaaaataa ttgttatttt cgttccatac
     1501 caaaataaat caaagttatc taaaaaaatc gtaaatgatt tatcaaaatc aaacatgatt
     1561 attctccttt aggctgacta ctactatatt tacccatctt ttcatttctt ttttctgatg
     1621 aaatattatc aatgcatttt tgtacttttt taaataaagt actgtttcga ctaccctctt
     1681 ttaaacctat tgaaactgcg gtatctactt taaacccttt attttgttca aatctaacaa
     1741 tcgctagatc ttgatttgtt tctaaaattc cttgggccac aggtaattcc gcagtaattc
     1801 catcgacttc accactttgt aaagcaacca gcatttcagg ataagattgt ttaggtgtag
     1861 catgatcaac tccattaatt tgatcaatta ctgtatcata acttgttgac atctgtccta
     1921 cgatcgtttt accagaaaat tgttgaatat cagtataatt tttactagca tcatcacccc
     1981 gtacgatcat aaccatttca gattgataat atggtgttgt aaaatctatt nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnncctt tttcacgctc ttcatcagct gtcattcctg caataatggc
     2101 atctatttcg cctgattcaa gtgcaggttg caaaccattc catgctaatt tttttataac
     2161 cagtttttta tcaagacttt ctgctatttc ctttgcgata taaacatcat agccatcagc
     2221 gtaaccactg ccttctaatt caactgcggt atcactttgt gtatttgtct gccagttaaa
     2281 cggtggataa ttacattcca ttccaactac aaatacatca ccttcatcaa tactcttatt
     2341 cccactatta ctctcacagc cactaattaa taaagctatt aataaaaaag ctaatatttt
     2401 cttcatttta tcgtttattc ctttcttttt aatattaaat attgaaataa gttaaaccat
     2461 tattttatcc tcctctagct attaaaagtc tagcatgcta ataattaata taggattaaa
     2521 gagttgtttt aaaatattaa aaagacataa ataaagaaca aattaaattc tttctaatta
     2581 acatttatta atgaacatat gcttttttag tagaataatg tattattgat gatttattta
     2641 aatatgtagc caactccttt attttctcag tggcttttta caacgacaaa aaagaattgg
     2701 atttcaaaac caattctttt ttataatatt aattttatta tagattaatc ctctgctaaa
     2761 actttatcct ttttcattaa aatcgttatt agaacaattg tactaattaa agaaattatt
     2821 gtaatcgcaa tgttgctggc atcacccgta tttacagaat gatttttgtt atttgtattt
     2881 gtgttagaac tattatttgt ttcttcaacc gttggttctt gtgatggatt atcagtttta
     2941 tcatcgcctg attgatcact atttttttgc aaagtcagat cgtaaactcc cactgtccca
     3001 gatacttcat aagcaacaac taattgtggt ttccctgtaa tactatcatt acctgcaata
     3061 aaatgaattc cttcaggtga atcatcacca ccaatatctg tgctaaaatc acgagaatta
     3121 atataattaa caaattctgt tttttctgga ttcgtaatgt cataaaccat tataccgcca
     3181 attctctcta atccaataaa agcaaatgtt ttatcttcga tttgaccaat tgtcacagct
     3241 tctgcttcag gacctttttt accagaacga tcatctattg tagcatcatc attagaacag
     3301 ttaaaattat ttggcaaata tgctgcagtt ttgacttcaa aatcatttcc tgttgtataa
     3361 atttcttgta atgactgctc atcagcttta aaaattgttg atgatcttcc cccaaataaa
     3421 taatcatttt cattattaag accatcataa tcactagtat cgaagaacgt gaccttacca
     3481 gttaaaccac tgttatcagc cgttatttta ccagttggtg aagtttttcc tttaccaaaa
     3541 tttacctcta tttcatttga ataaccattc cagtcacgac tatctccttc attagcagtt
     3601 attaaataat cgattccatc aaccgtgtaa acagaaatag catcaggcat ccgaattcct
     3661 tttaaagaat catatgtttg tgcattatat ttttcatctt ttttatcaat atcaattgca
     3721 attttactat aatcttcaaa tcctactgaa taaatatctt ctatttcaag actatttaaa
     3781 tctaatttag caatcgcatt agcttcttgt aatgtaatat atgctttaga atcactaact
     3841 gcaatatatt ctggttctaa atctgttgat ggcaaaacac cttttttcaa aacaattcct
     3901 ttttctatta atgcatctcg ttgactatca aaagctgtga agtcaacagt ttttgctttg
     3961 tttgttttgg tattaataac gcttatacta ccttttggat caactgctcc agtatacccg
     4021 tcccgaggtt ctccttcgtt agctgttaaa atttgtccat catcattagt aaagacaacc
     4081 atatctggtt gaacacctac ttcgaacaat tgctttaaag ttattgttcc atcttgatta
     4141 caatcaaata aagctactct accattagca ttagtctcct taccttgaat cgcaacagcc
     4201 aatgaggttc catcatgact aactgctact gatgtcatat caccatatgt aaaatcagca
     4261 ctttgtacta acgaactaat atcaatgtca ttaccgtcca ataagtctat tttatcctta
     4321 ttttcgatat tttttagggt aattgctgtt aaatttccag attgtccatt aacagcataa
     4381 gcccatcctg tcactgtgtt ataatcaact atttcagtta ctcccccatc actgttagtt
     4441 gttcctgaat cataacgagc tgactgttct agcgctaata atgctgaatt gttttcataa
     4501 ccagttgttt tttcaccacc gtcactattt ttagtaacag taaaagcatc gatttcttta
     4561 ctatcaggat tagtttgatc accagcaaga ttataaacct ttaccgatat tcgattattt
     4621 tcaacattaa atactgcata actaggacta tactcttgat tccattcttg atttccaact
     4681 ggtaatgaat ctccttctag gtaagccgca gaaccacttt taccattagc taagattgga
     4741 taatcagcat tattattttt atctaatgtt tcaaacggtg tataatattg catatctgaa
     4801 caacagttcc ccgtaatata aattgttcct tgaggattat taagtgtatc taatctttca
     4861 ctatttcttt gcccatcttt taaaacatag ctacgtgaat aaacatgatc atgtccgcct
     4921 aagacaaaat ccacattata ctcatccata agtttttcaa atcctgcatc aacatagttt
     4981 tcaatatcac tatctgctgc atgtttagca actgtttgag tagatttatg atgagtgact
     5041 ataatccatt gatattgatt ttggtattca ctagttgcac tctttaatgt ttgagcaaaa
     5101 ttattgacga ttgcttctgc ctctgaatta tctctagagg cacttgaaac attaggattg
     5161 tttgcatttt cttcgtcatt tcccccagga taagcactag tatttaaagt gacaaataaa
     5221 acattattat ataaatagta atagtttcct tgagtttcca tatcacggcg ttcactaaaa
     5281 tcatattgac catcactatt tggtgtcacc ccattagaag ctgcattatt tttaatttca
     5341 ttttcagtag cctgaatata atttccgtga ctttgactag ttccactatt ttgtcctcta
     5401 aacgttgttt taacttcagt taatgattcc atttgatttg gtaaattaaa atgatctgcg
     5461 aacatatagt gtctatcatg atttccaaca gctggagcat atggaattga tgccatttct
     5521 tctggtgcaa aaaaagcctc atactcgctt gattttcccc agttttgatc ttcaacttga
     5581 tcccctgctg acactactaa gtcaacacct tgtttttcta aagtttccat catagttgcc
     5641 caaccggttt gatttgtttc atcacttggg ttccaacctt cagaattaat ttccttgttt
     5701 tcctttattt gtggatcgct tgtaaaacca aaagtaaatt cattgcttga tggagttgta
     5761 taattatatt cctttgacca agttgttccc ccatcatttg aaatataata agtatactta
     5821 gtatctggtt ttaaattact gattgtcgtt ttacatacta attttccagt atcagtatat
     5881 ttatcacttt ttatttcggt tggcgaagtc aatggtttgg ctgttacatc ataagtttga
     5941 tcaccaaact gaatttttgc taccgtcgtt cctgctggtg cataccaatt tagattgatt
     6001 gatgtcgttg ttgcccctgg ctgaagtgtt aaactatcca cactaaatga accagtatgg
     6061 ctttcacctg caacctcact ataagtatct tgg
//
LOCUS       DAOMTA010000071         6086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_236_length_6086_cov_2.869508, whole genome shotgun sequence.
ACCESSION   DAOMTA010000071 DAOMTA010000000
VERSION     DAOMTA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_236_length_6086_cov_2.869508"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PLK68_03065"
     CDS             <1..169
                     /locus_tag="PLK68_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRM89424.1"
                     /translation="CDCSKEKMGKALITVGKDELQAMIDEDHGCELSCQFCNKKYQFS
                     EEELKEIKNRL"
     gene            402..767
                     /locus_tag="PLK68_03070"
     CDS             402..767
                     /locus_tag="PLK68_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HRM89425.1"
                     /translation="MTAAEVTIEATGDKTDVMKSTVTYDFTGYVTESTPIDTYWLPKV
                     KSINVDYDSLKGGSAKYTVDGEKIILKIELDYGEADFDELKKAKLVTTTDSDKKIVYI
                     SLEETIKEQEKGGLTSKEK"
     gene            977..1957
                     /gene="dusB"
                     /locus_tag="PLK68_03075"
     CDS             977..1957
                     /gene="dusB"
                     /locus_tag="PLK68_03075"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRM89426.1"
                     /translation="MFKIGNVEIENPVVMAPMAGITNMAFRKIIKDFGAGLVYSEMVS
                     DKALCYGNAKTIEMLQVDDGEHPVSIQLFGGEVETMVQAAKYIDKHSNCDIIDINMGC
                     PVNKVLKADAGSKLLLYPDKIYEIVKGVVDNVTKPVTVKIRSGFDKEHINAIEVAQLI
                     EKAGASAIAIHGRTRSQMYEGHADWEIIRQVKEAVKIPVIGNGDIKSVEDAKRMLEEI
                     KVDAVMIGRAALGNPWLIKQVVESLKTGETISEPTYQEKITQCLAHARRLMEIEPEKV
                     AMFQMRGHAPWYIKGLKSSARVKNELSKINTFEELEIILKEYQEYLDQLD"
     gene            2019..2867
                     /gene="folD"
                     /locus_tag="PLK68_03080"
     CDS             2019..2867
                     /gene="folD"
                     /locus_tag="PLK68_03080"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRM89427.1"
                     /translation="MIISGKEISVKIKDQLKEEVSKIKETYPRLPKLVVILVGDNQAS
                     QTYVRNKERGCQYIGIESEILRHDASFSEIELLQEINDLNNDDTVDGILVQLPLPQHI
                     NEEKVLDAIVPSKDVDGFHPENVAKLFLGQHSLVPCTPKGMMVLLEEINYDLAGKEVV
                     IVGRSNIVGKPVALLCLQKNATVTIAHSQTKDLKAVCSRADVLIAAIGKPKFFNHEYV
                     KDGAVVLDVGINRDENNKLCGDVDFDDVKDKVSAITPVPGGIGPMTITMLMKNTIEAF
                     YHRNGD"
     gene            2857..3048
                     /locus_tag="PLK68_03085"
     CDS             2857..3048
                     /locus_tag="PLK68_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRM89428.1"
                     /translation="MVTNMEYKLNDIVEMKKAHPCKKSTQWQIIRMGADIKIKCLGCG
                     AIVMFPRREFEKKLKKIIE"
     gene            3213..>3550
                     /locus_tag="PLK68_03090"
     CDS             3213..>3550
                     /locus_tag="PLK68_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HRM89429.1"
                     /translation="MTIKELLEQADTVLKDEKLREMFKRCFLSTLETSVKREGDKTYI
                     ITGDINAMWLRDSSCQVNHYLKYIHNDSELQSIIKGLIKAQVEYVLWDPYANAFLNTE
                     DVEREYHNTT"
     assembly_gap    3551..3579
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3580..4489
                     /locus_tag="PLK68_03095"
     CDS             <3580..4489
                     /locus_tag="PLK68_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HRM89430.1"
                     /translation="ERKYELDSLCYPVKLSYQYYKETGDKSIFNQQYLHFIRTLLNVF
                     ECEQNHSQNSSYTFEREEAKKWNLRKTETLQNKGRGFETRYTGMIWSAFRPSDDACTF
                     NYNIPGNMFCSVVLGYLKEIVELVYQDEYLQERIVDLKFQIDYGIELFGIVRHPKYGK
                     IYAYETDGYGNHVLMDDANVPSLLSIPYLGFADANDEIYKNTRAFILSKENPYYFEGN
                     RAKGIGSPHTWSEYIWPIALSMQGLTSLLQHEREALIQTIIDNTGGTGYCHESFDVND
                     DTQFTRPWFCWADSLFAELVIKTYFE"
     gene            4636..5976
                     /locus_tag="PLK68_03100"
     CDS             4636..5976
                     /locus_tag="PLK68_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89431.1"
                     /translation="MKLIMSLLEKYLMPFSNKITSNKLLNAIKDACILTSPFTVVGSL
                     AGLVSQQANYWFGEWGIPLDGILGSIINAFTNINTVAMGLTGLVVVIASSYNYANQLK
                     GLNKGDKCVPLVACIVSLIAYIATIPNAINVGDSTITAFQINFFNYEGMFAGMIIGLA
                     SSYMYYKLVNSKFTIKLPGQVPPMVLSSFLSIIPFFVIITVFSVIKEIVVLAGFDSIQ
                     QLITQFIITPLNGIGTGLPAVILVIIIMQLLWFFGVHGFSIMWGLISVLWMPIFYEHI
                     QIFVETGSFDKITQVAPNTLCNVYAMIGGSGSTLALVVLLLVLGKKGSAERSIGKVSI
                     IPGLFGINEPVTFGLPIVLNPIMFIPFVFVPVINAIIGYFATSWGLVNHLVVLNSGVE
                     PIFVNAWVLGAFTLSPVILCIVLFILDLVLYYPFVKLQIKQNTLEAQNEGAAVE"
BASE COUNT         2125 a          668 c         1218 g         2046 t
ORIGIN      
        1 atgtgattgt tcaaaagaaa aaatgggtaa ggctttgatt acagttggta aggatgagtt
       61 acaagcgatg attgatgagg atcatggttg tgaattaagt tgccagtttt gtaataaaaa
      121 atatcaattt agtgaagaag aattaaaaga aattaaaaat agattatagt agaatagtga
      181 aaactattct attttttgta attttatgtg attattttgc tacaaatgta ctattatggt
      241 attttttagg taaattttgt gctatgataa tggtatagac aaataaagtg agggaaaatc
      301 aaatgaaaaa actattaagt gtaatgttac tggcattgct attaacagga tgtggtggag
      361 aagaaaaaac cactactatt tgtaaaggaa atattgatta aatgacagca gctgaagtta
      421 cgatcgaagc aactggtgat aaaactgatg tcatgaaaag tacagttact tatgatttta
      481 ctggttatgt aactgaaagt acaccaattg atacttattg gcttcctaaa gtgaaatcta
      541 ttaatgttga ttatgattca ttaaaaggtg gttcagctaa atatactgta gatggtgaaa
      601 aaattattct taaaattgaa ttagattatg gtgaagctga ttttgatgaa cttaaaaaag
      661 ctaaattagt tacaactact gatagtgata agaaaatcgt gtatatttct ttagaagaaa
      721 caattaaaga acaagaaaaa ggtggactta ctagtaaaga aaaataaaaa gaaaggcggg
      781 ggaagccttt ctttttttgt tggaaaccaa catatttagg aatataacta actcgataac
      841 ttacgtctat ttcggggagg aaaatagcgg ggaagggtaa gttatctata aatagtatgg
      901 ctatgaagaa atattttata catttatatt tctttttttt taatttattg ataagatact
      961 tttgaggagt gacataatgt ttaaaatagg aaatgtagaa atagaaaatc cagtagtaat
     1021 ggcaccaatg gcagggatta ctaatatggc ttttcgtaaa attattaaag attttggtgc
     1081 cggacttgtc tattcggaaa tggttagtga taaagcgcta tgttatggaa atgctaaaac
     1141 aatagaaatg ttacaagtag atgatggcga acatccagtt agtattcagc tatttggcgg
     1201 tgaagtagaa acaatggttc aagcagccaa atatatcgat aaacattcta attgtgacat
     1261 tattgatatt aatatgggat gtccagtaaa taaggtttta aaggccgatg ctggtagtaa
     1321 attattatta tatcctgata aaatttatga gattgttaaa ggtgtcgttg ataatgtaac
     1381 taaacctgtt acagtaaaaa tacgtagtgg ttttgataaa gaacatatta atgcaattga
     1441 ggttgctcaa ctaattgaga aggctggtgc tagtgctatt gcaattcatg gtagaactcg
     1501 ttcgcaaatg tatgaaggtc atgctgattg ggaaataatt cgtcaagtca aagaagctgt
     1561 gaaaatccca gtaattggca atggcgatat caagagtgtt gaggatgcta aaagaatgct
     1621 tgaagaaata aaagttgatg cagttatgat tggtcgtgcg gccttaggaa atccatggtt
     1681 gattaaacag gttgtagagt ctttaaaaac tggtgaaaca attagtgaac ctacttatca
     1741 agaaaaaatt acgcagtgtt tagcacacgc acgacgttta atggaaattg aaccagaaaa
     1801 ggttgctatg tttcaaatgc gtggtcatgc gccatggtat atcaagggat taaagtcttc
     1861 agctcgtgtg aaaaatgaat tatctaagat taatactttt gaagaacttg aaattatttt
     1921 gaaagagtat caagaatatt tagatcagtt ggactaagag ttgcaacaag tttatatttt
     1981 ggtataattg aaataataat taaaggggga tttgagtagt gattattagt ggtaaagaaa
     2041 tatctgtaaa aataaaggat caattaaagg aagaagtaag taagataaaa gaaacatatc
     2101 cacgtctacc taagttggtt gtaatactag taggggataa tcaagctagt cagacatatg
     2161 ttaggaacaa agaaagaggt tgtcagtata ttgggattga atcagaaatc ttgagacatg
     2221 atgcatcttt tagtgaaatc gaattattac aagaaattaa tgatttaaat aatgatgata
     2281 ctgttgatgg aattttagtg caattaccat tgccacagca tattaatgaa gaaaaagttt
     2341 tagatgcgat tgttcctagt aaagatgttg atggttttca tcccgaaaat gtggctaaat
     2401 tatttttagg acaacatagt ttggttcctt gtacaccaaa agggatgatg gttttattag
     2461 aagaaattaa ttatgatttg gcgggtaaag aagtagtaat tgttgggcgc agtaatattg
     2521 ttggtaagcc ggtagcctta ttgtgcttac aaaaaaatgc aactgttaca attgcacata
     2581 gtcagacaaa agatttaaag gcagtttgtt cacgtgctga tgtacttatt gcagcaatag
     2641 ggaaaccgaa attctttaat catgagtatg ttaaagatgg tgcagtagta ttagatgtag
     2701 gaattaatcg tgatgaaaat aacaaattat gcggtgatgt tgattttgat gatgttaaag
     2761 acaaagtgtc agcaattact ccagttccag ggggaattgg accaatgacg attacaatgt
     2821 taatgaaaaa tacaattgaa gctttttatc atagaaatgg tgactaatat ggaatataaa
     2881 ttaaatgata ttgttgaaat gaagaaggct catccatgca aaaagtcaac acaatggcaa
     2941 attattagaa tgggtgcaga tattaaaatt aaatgtttgg gatgcggagc gattgtaatg
     3001 ttcccacgcc gtgaatttga aaaaaaatta aagaagataa ttgagtagta tggatgatga
     3061 tttattagaa gatgaattag ataatgtctt agatatatat tggcactttt agtgccaatt
     3121 tttttgtttg attaatcttg taaaaaatat acagtattgg gaatagacaa tttaaggaaa
     3181 gatagctatg attgaaacag gaggaaagca ttatgacgat taaggaatta ttggaacaag
     3241 ctgatacagt attgaaagat gaaaagttac gcgaaatgtt taaacggtgc tttttgtcta
     3301 cgctagaaac aagtgttaaa cgagaaggtg ataagactta tattattacc ggagatatta
     3361 atgcaatgtg gttacgtgat tcaagctgtc aagttaatca ttatttaaaa tatattcata
     3421 atgatagtga attacagtca ataatcaaag gattaattaa agctcaagtt gaatatgttt
     3481 tgtgggatcc ttatgcaaat gcatttttaa atacagaaga tgttgaaagg gaatatcata
     3541 atacgactat nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gagcgaaagt atgaattgga
     3601 ttcattatgt tatccggtaa aattaagtta tcaatattat aaagaaactg gtgataaaag
     3661 tatttttaat caacagtatt tacattttat tagaactctt ttaaatgttt ttgaatgtga
     3721 acaaaatcat agtcaaaatt catcatatac gtttgaacga gaagaagcaa aaaaatggaa
     3781 tttaagaaaa accgaaaccc tccaaaataa aggacgtggt tttgaaacac gttataccgg
     3841 aatgatttgg agcgcattta ggccaagtga tgatgcatgt acttttaatt ataatattcc
     3901 tggcaatatg ttttgcagtg ttgttctggg atatctcaag gaaattgtgg aattagtgta
     3961 tcaggatgaa tatcttcaag agcgaattgt tgatttgaaa tttcaaattg attatggtat
     4021 tgaattattc ggtattgtta gacaccctaa atatgggaaa atatatgcct atgaaacaga
     4081 cggttatggg aatcatgtat taatggatga tgccaatgta ccaagtttat tgtcaattcc
     4141 atatttagga tttgcggatg ctaatgatga aatttacaaa aatacacgag cgtttatttt
     4201 aagtaaagag aatccatatt attttgaggg gaacagggca aagggaattg gatcgccaca
     4261 tacctggtct gaatatatat ggccgattgc gctatctatg caaggattaa caagtttatt
     4321 acagcatgaa agagaagcat tgatccagac tattattgat aataccggag gtactggata
     4381 ttgtcatgaa agttttgatg taaatgatga tactcagttt acaagaccat ggttctgctg
     4441 ggctgattct ttatttgcgg agcttgtaat caaaacttat tttgaataaa taatatctat
     4501 gataaaatag aaaaatgata agaagatttc gatcttcttt ttttgttttt tgattaggaa
     4561 aaattttaca agttctgtaa ttctcttatg gaagcgcttt gtatatactt ttatatgaaa
     4621 gagggaggaa ataaaatgaa attaattatg tcattattag aaaagtattt aatgccattt
     4681 tctaataaaa ttacatcaaa caaattgctt aatgcaatta aagatgcttg tattcttaca
     4741 agtcctttta ccgtggtagg ttcgttggca ggtctagtaa gtcagcaggc aaattattgg
     4801 tttggagaat ggggaatccc attggatggt attttaggaa gtattatcaa tgcctttaca
     4861 aatattaata ctgtggctat gggtttaact ggattagttg ttgttattgc ttcttcatac
     4921 aattatgcta atcaattaaa agggctaaat aaaggagata aatgtgttcc attagttgct
     4981 tgtattgttt ctttgattgc ttatatagca actattccta atgctattaa tgtaggtgat
     5041 agtacaatta cagcttttca aatcaacttc tttaattatg aaggaatgtt tgcgggaatg
     5101 attattggat tggcttcatc atatatgtac tataaactag ttaattcaaa atttacaatt
     5161 aaattacccg gacaagtacc gccaatggta ttaagttcat tcttatcaat tattccattc
     5221 tttgttatca ttactgtatt ttcagtgata aaggaaattg ttgttttagc aggatttgat
     5281 tcaatccaac aattgattac tcaatttatt attactccac taaatgggat tggtacaggt
     5341 ttaccagcag ttattttagt aattataatt atgcaattat tatggttctt tggagttcat
     5401 ggtttttcaa ttatgtgggg attaatttct gtattgtgga tgccaatttt ctatgaacat
     5461 attcaaattt ttgttgaaac aggttctttt gataaaatta cacaagtagc accaaatact
     5521 ttatgtaatg tctatgcaat gattggtggt tctggctcta ctctagcatt agttgtatta
     5581 ttattagtat tagggaaaaa aggttctgct gagagatcga ttggaaaggt ttcaattatt
     5641 cctggtttat ttgggattaa tgaacctgta acattcggat taccgattgt attaaatcca
     5701 attatgttta ttccatttgt atttgtacca gttattaatg caattattgg atattttgca
     5761 acatcatggg gattagttaa ccatttggtt gtattgaatt ctggggtgga accaatcttt
     5821 gttaatgctt gggtattggg ggcatttaca ttgagtccag ttattttatg tatagtatta
     5881 tttattttag atttagtttt atattatccg tttgtaaaat tacaaatcaa gcaaaatact
     5941 ttagaagcac agaatgaagg tgctgccgtt gagtaagata cattttattc cccatgttca
     6001 ttgggataga gagtggttac gttcaacgga ttcatcaaga attaaattag tctattttta
     6061 tgatcgactg attgaaatgt tagaga
//
LOCUS       DAOMTA010000072         6085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_237_length_6085_cov_2.167993, whole genome shotgun sequence.
ACCESSION   DAOMTA010000072 DAOMTA010000000
VERSION     DAOMTA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_6085_cov_2.167993"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLK68_03105"
     CDS             complement(<1..213)
                     /locus_tag="PLK68_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRM89432.1"
                     /translation="MKVNDYLNELRKIKDVAMATVDSDGYPQIRIIDVMAVENNNLYF
                     LTARGKNFYQELLDKNFVSLVALTKDY"
     gene            complement(435..1001)
                     /locus_tag="PLK68_03110"
     CDS             complement(435..1001)
                     /locus_tag="PLK68_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRM89433.1"
                     /translation="MCGRYFLELKALMELEERIDYTFERDLLIAKDYYPSNIVPIIID
                     RDNKLELKKAKWGFSTFDNKLIINARSETLLEKPFFKKEVITHRCLIPASGFYEWDGH
                     KHKFTFEKRSLLLMTGIYRNVNGQTEVTIVTTKANKSMCEIHDRMPLILEEHLNHDWL
                     ENRHIEALLRTVPQSLTITSGFLQSSLF"
     gene            complement(1011..>1501)
                     /locus_tag="PLK68_03115"
     CDS             complement(1011..>1501)
                     /locus_tag="PLK68_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRM89434.1"
                     /translation="FDHHSDMIKPMFGDILSCGSWILNALKDNQYLKQIILIGIAQEQ
                     VDLIDPKYRERVIYLCDNNLGDLKVWKIIDELLKQYPVYFSIDKDVLSEQVVTTDWQQ
                     GQMRLIEFKLILADLIKRGDVIGIDICGECCNEVAMLSEIKNDDSLNAQILEFLKCEL
                     VK"
     assembly_gap    1502..1549
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1550..1759)
                     /locus_tag="PLK68_03120"
     CDS             complement(<1550..1759)
                     /locus_tag="PLK68_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRM89435.1"
                     /translation="MDFTGIYELESFYKHKNINWIDCLDIKGTRGYCSEEAKAKIIKR
                     IGDYLPAGIHFIDSGNFHYVSEFWIR"
     gene            complement(1812..2369)
                     /locus_tag="PLK68_03125"
     CDS             complement(1812..2369)
                     /locus_tag="PLK68_03125"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRM89436.1"
                     /translation="MMMVKRCSWVDEKSEIYKNYHDHEWGVPVYDDEKLYEMFLLETF
                     QAGLSWITILKKREAFRVAFDNFDVIKIANYDDKKVMELLANEKIIRSKRKIGAAINN
                     AKIFINIQTEFGSFSDYLWGFSNHQIIKNKDNNFKTTTKLSDDISMDLKKRGMSFVGS
                     ITIYSYLQAIGIVDDHELECFCYKH"
     gene            2570..3913
                     /locus_tag="PLK68_03130"
     CDS             2570..3913
                     /locus_tag="PLK68_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRM89437.1"
                     /translation="MNNNNNDVTKKYGLFTAITMIVGICIGSGIFFKSDNVLIATNGS
                     IALGVLSFILAAISIIFGCLTIGELASRTDKVGGLISYAEMFLTRKVACAMGWFQTFV
                     YYPTITSVVAWVIGVYINILFNLQASLEFEILIGYIFLLLCFVYNILLPKFGAFIQNS
                     TTLIKLLPLFILGILGIIFGDPINGLANTSINTLSGTGWISALGPIAYSFDGWIVTTS
                     ISHELKDAKKNMPKALMLGPLIVLSIYVLYFIGISCYVTPEVVMTLGDRHVSLAAQNL
                     FGPAFSKMIIIFIIISVIGTVNGLVIGYIRMPYSLSLRKNMFPFSKHLRKIDKRFQMP
                     LNSAICSLIICTIWMFVHYLCTKYNLLYNSDVSEGAIGISYIFYTLLYIRVFKMYLDK
                     EIKSKFKGLVCPILATIGSLIILSGGIQNKLFIFYIIFCILLFLYSLYYYQKNNH"
     gene            complement(3954..4175)
                     /locus_tag="PLK68_03135"
     CDS             complement(3954..4175)
                     /locus_tag="PLK68_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89438.1"
                     /translation="MQKDIFEDAVKLMRCYFISDLRILKKEVYEMLYIVDFKQYDIPN
                     LQQFFAYVFDREISSYEDIEEFLWKESTL"
     gene            complement(4621..4893)
                     /locus_tag="PLK68_03140"
     CDS             complement(4621..4893)
                     /locus_tag="PLK68_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89439.1"
                     /translation="MKKTKSLVGRKYGKLLVLAETNKLEARYKVWECRCECGKITFVN
                     TKKLKRGTITNCGCIPKNKAKKGQKFGDLWQFLQQKKRTQKLVKLG"
     gene            complement(5216..5809)
                     /locus_tag="PLK68_03145"
     CDS             complement(5216..5809)
                     /locus_tag="PLK68_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM89440.1"
                     /translation="MTNIYGYARVSSKDQNEARQIIALSQFPVKKENIYIDKFSGKDF
                     DRPKYSELIKILKEQDILVIKEIDRLGRNYEEILEQWRVITKEIKADIVVLDMPLLDT
                     RTRKENLTGTFIADLVLQILSYVAETERQSIKQRQREGIEAAKKRGVKFGRPCIPIPE
                     EFYDLKEKWLNKKITSREAATTINVSQDTFLRWVHLK"
BASE COUNT         1994 a         1066 c          691 g         2286 t
ORIGIN      
        1 ataatctttt gttagcgcaa ctaatgaaac gaaattttta tcaagtaatt cttgataaaa
       61 gtttttcccc cgagcagtca aaaaatacaa attattattt tccacagcca ttacatcgat
      121 gattctaatt tgtggatagc cgtcactgtc tacagttgcc attgctacat cttttatttt
      181 tctaagttca tttaaataat cattaacttt catcactctt acctccgctc gtctttaata
      241 tacactagtt gatttttata tgaaagaagg cacttttttg taacctagtt actttaacga
      301 aactaaaaaa ctatagctag tcgtgtacaa cttcatagct atagctctaa taataatcaa
      361 acttatactc tataatattg tattaggcac accataagta tactgttcta atatcaaaat
      421 aacaaatcat atgcctaaaa taaactactt tgcaaaaaac ctgaagtaat cgtcaaagac
      481 tgtggtactg tccttaaaag tgcttcaata tgacgatttt ctaaccagtc atgatttaaa
      541 tgctcctcta aaatcaatgg cattcgatca tgaatctcac acatactttt atttgctttt
      601 gttgtaacaa tagtcacttc tgtttgcccg ttaacatttc gataaattcc tgtcattaac
      661 aataacgatc gtttttcaaa agtgaactta tgtttatgac cgtcccattc atagaagcca
      721 ctagctggaa taaggcaacg atgagtaatc acttcttttt taaagaatgg tttttccaat
      781 aaagtttcac ttcgcgcatt aataattaat ttattatcaa aagtactaaa gccccatttt
      841 gcctttttta attctaattt attatctcga tcaataatga ttggtacaat attacttgga
      901 taataatctt tagcaattaa taaatcacgt tcaaatgtat agtcaattct ttcttccaat
      961 tccatcaagg cttttaattc taaaaaatat ctgccacaca taataatctc ctattttacc
     1021 aattcacatt ttaaaaactc taagatttgc gcatttaatg aatcatcatt ttttatctca
     1081 cttaacatgg caacttcatt acaacattca ccacatatat caatcccaat aacatcgcca
     1141 cgtttaatta aatctgctaa aattaattta aattcaatta accgcatctg cccctgctgc
     1201 caatcagtcg tgactacttg ttcacttaaa acatctttat caattgaaaa ataaacagga
     1261 tattgtttca aaagttcatc aattatcttc caaactttta aatcaccaag attattatca
     1321 cacaaataaa taacgcgctc cctatactta ggatcgatta aatcaacttg ttcttgtgca
     1381 attccaatca aaatgatctg ttttaaatat tgattatctt ttaaagcatt taagatccat
     1441 gatccacagc ttaaaatatc cccaaacatc ggtttaatca tatcactatg atgatcaaag
     1501 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cttatccaaa
     1561 attcacttac ataatgaaaa ttacctgaat caataaaatg tattcctgca ggcagataat
     1621 cacctattct tttaatgatc ttcgcttttg cctcctcact gcaataacca cgtgtcccct
     1681 taatatctaa acaatcaatc caattaatat ttttatgctt ataaaaactt tctaactcat
     1741 aaataccagt aaaatccatg acagttaatt tcttttcatt tacaatactt accccataaa
     1801 accaactcca tttaatgctt ataacaaaaa cattctaatt catgatcatc aacaatacct
     1861 attgcttgta aatatgaata aatagttatt gagcctacaa aactcattcc tctttttttt
     1921 aaatccatcg aaatatcatc agataacttc gttgttgttt taaaattatt atccttattt
     1981 ttaataattt gatggttact aaaaccccaa agatagtcag aaaaactacc aaattcagtt
     2041 tgaatattta taaaaatttt agcattatta attgctgcac caatttttcg tttacttctt
     2101 attatttttt catttgctaa taactccatt acttttttat catcatagtt agctatttta
     2161 attacatcga aattatcaaa tgccactcta aaagcttctc gtttttttaa aatagttatc
     2221 catgataatc ctgcttgaaa tgtttctaat aagaacatct catataattt ttcatcatca
     2281 taaaccggta caccccattc atgatcatga taatttttat aaatttctga cttttcatct
     2341 acccatgaac acctttttac catcatcatt ctctcctttt taactatttt atcatgctta
     2401 taagaataag caaatattac acaaaaaaaa actatcccag ttaaaattac tcattcatca
     2461 tatttaactt tttaaatatt ttttccagca gctaaaataa taaattatat tacttttaat
     2521 attgtttagt ttttagtaca atatttcaag atgttagagg agatttaaaa tgaataacaa
     2581 taataatgat gtaacaaaaa aatatggatt atttacagcc attacaatga ttgttggaat
     2641 ttgtattggc tcgggtatat tctttaaaag tgataatgtt ttaattgcta caaacggcag
     2701 tattgcacta ggcgtgctat catttatctt agccgcaatt tcaatcattt ttggttgtct
     2761 tacaatcggt gaattagcat ctcgcactga taaagttgga gggctaatct catatgctga
     2821 aatgttttta actcgtaaag ttgcatgtgc tatgggctgg tttcaaacct ttgtttacta
     2881 tcctacaatt acttctgttg tagcttgggt cattggagtt tatataaaca tattatttaa
     2941 tttacaagcc agtttagaat ttgaaatttt aattggttat atctttttac tactttgttt
     3001 tgtttacaat attcttttac caaaatttgg tgcttttatc caaaatagta caaccctaat
     3061 aaaactactc cccttattta ttttaggaat attgggaatt atttttggtg atcctattaa
     3121 tggtttagca aatactagta tcaatacact aagtggtact gggtggattt ctgcccttgg
     3181 tcccattgcc tattcatttg atggctggat cgtaacaact tctatatctc atgaactaaa
     3241 agatgctaag aaaaatatgc ctaaagcatt aatgctaggc ccattaatcg ttttaagtat
     3301 ctatgtttta tactttattg gtatctcatg ttatgttaca cctgaagtag ttatgacttt
     3361 gggggatcgt catgtcagtt tggcagcaca aaatttattt ggtccagcct ttagtaaaat
     3421 gattatcatt tttataatta tttcggttat tggaactgtt aatggtttag tcattggcta
     3481 tattcgcatg ccatattcat tatctttacg taaaaatatg tttccctttt ctaaacattt
     3541 gcgtaaaatc gataaacgtt ttcaaatgcc cttaaattca gctatctgta gcttgattat
     3601 ttgtacgatt tggatgttcg tccattattt atgtactaaa tacaacctat tatataatag
     3661 tgatgtatct gagggagcta tcggaatatc ctatatcttc tatactttat tatatataag
     3721 ggtatttaaa atgtatcttg ataaggaaat taagagtaaa tttaaaggtt tagtttgccc
     3781 gattttagca actatcggct cattgattat cctatctgga ggtattcaaa ataaactatt
     3841 tattttctat attatatttt gtattttact ttttctatac tcattatatt actatcaaaa
     3901 aaataatcac taaataataa accatctctt agaatcaaga gatggtttat tatttataaa
     3961 gttgattcct tccataaaaa ttcttctata tcttcataac ttgaaatctc tcgatcaaaa
     4021 acataagcaa aaaattgctg caaattcggt atatcatatt gtttaaaatc cacaatatac
     4081 agcatttcat atacttcttt cttcaaaatc cgtaaatctg aaataaagta acaccgcata
     4141 agttttacag catcttcaaa aatatctttt tgcataatta accatccttt caagtcatat
     4201 atctatttct tctctttatc ctgactccgc ttgatttgta atttcccatt ggccttatta
     4261 acttcaaaaa taaacggatt tttctggaac cagagtaaat cagcagcctt taaattccac
     4321 tgttcataag caataataaa agtttgataa actagctctt cagcttctct tcgagtctct
     4381 agagccgatt cgaaatttga atatgttcct aaatcaaaac gtttaccttt aaaaccaata
     4441 ctagctcgat atttattatt tttcagttta taaataccgc atgctccact agtattattt
     4501 ctttttaatt tttgtgagtt aataaactct atacatgtac catcaactaa atgaagatta
     4561 ttataaacac tttcagtaat ctcttttttc tacacccaca accgacatac tgcccgtgca
     4621 ttagcccagt ttcactaact tctgtgttct ttttttctgt tggagaaatt gccataagtc
     4681 gccaaacttc tgtccttttt tagccttgtt tttaggaata cagccacaat tagtgattgt
     4741 ccctcgtttc agtttttttg tattaacaaa tgttattttt ccacactcac atctacattc
     4801 ccatacttta tatcgagctt ctaatttatt tgtttcagcc aagacgagta atttaccgta
     4861 ttttctgcca actagacttt tagtcttttt cattaattga atttactttc agcttagaag
     4921 cagataaaca aatttataat taaacaacat aactgtgtcc tcatttattc gttcatccct
     4981 cctcaataat tctagtacta ataatgtaaa attgtgttta tacttgtctt gcacatagta
     5041 caaattcgtc cgcataaagt agacttattc attcgaaaag ataaagcttt atttttaacg
     5101 aaaaaactat aaaaaacaca aatttctttc tactatgatt aattttgatg tactatctgt
     5161 caattttcat tatacatttg ttgaacgata aagtctacta ttttcataat aatcatcact
     5221 tcaaatgaac ccatcttaaa aatgtatctt gagaaacatt aatagtagtg gctgcttccc
     5281 ttgaggtgat ttttttattt aaccattttt cctttaaatc ataaaactct tctggaatag
     5341 gaatgcatgg tctaccaaat tttacgcctc tttttttagc agcctcgatt ccttcacgct
     5401 gtctttgctt gatactttgt cgttcggttt cggctacata tgaaagaatt tgaagtacta
     5461 gatctgcaat aaatgtccct gtaagatttt cttttcttgt tctagtatct agtagtggca
     5521 tatctaaaac aacgatatct gcttttattt cttttgtaat gactcgccat tgctctaaga
     5581 tttcttcata gtttctgcct agacggtcaa tctctttaat cactaaaata tcttgctctt
     5641 tcaatatttt aattaattca ctgtacttgg gtctatcaaa atccttaccg ctaaacttat
     5701 caatataaat attttccttt tttactggaa attgtgataa agcaataatt tgccttgctt
     5761 cattttggtc ttttgaagaa actcttgcat aaccataaat atttgtcata aactaaaacc
     5821 tcctaaattt atgcagtatt ataaaaatat ctgcataaat attattaaca agttatgcta
     5881 aatctatatt actgaatgta aaaaaagtta gtaataagtc aatgttacta actagctaga
     5941 aatattatta ctttataaat tgattttaat gaataaataa tttattaaaa tcaaaacagg
     6001 aaatatcaag ataggagaaa attatgacta aatcaaatac aattattgaa ttaaaaaaac
     6061 aaggtgtagt tgcagtaatt agagg
//
LOCUS       DAOMTA010000073         6037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_242_length_6037_cov_2.178703, whole genome shotgun sequence.
ACCESSION   DAOMTA010000073 DAOMTA010000000
VERSION     DAOMTA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242_length_6037_cov_2.178703"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLK68_03150"
     CDS             <1..588
                     /locus_tag="PLK68_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRM89441.1"
                     /translation="LRFNDDFDFRSEFVGKWNKKIHSLRVAGIIVAILMIICAILCMI
                     YPVKSVTIIGTIATGLILILGIYQIIDYIAAPPLFRGPGGLVSAICNLLIGLLLICSP
                     IEMTINTFAFIFGFILMVYGLNKLSFAHQLSFFGIESYGWVIFTGIINVLAALAFIIA
                     PLMSTVVLNYIVAAYLLVGGIALLVEVIAMHDLKL"
     gene            739..1605
                     /locus_tag="PLK68_03155"
     CDS             739..1605
                     /locus_tag="PLK68_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HRM89442.1"
                     /translation="MFDFFKKKKDPVEESGKLEMNKEMFLSTMKVITDYTQRDVIKIE
                     LSNDECSIFDSKFGGLPYLGVDDEVPLSTDNKQLRLLGQINFADLPMNSYQLEAGLLQ
                     FWAMDNDLYGLDFENMISQAESRVIYYSSIDKTVSESQILNKYHPEGEGYFPIVDNFK
                     LHFIGSEEGISIGDYHFDGLFTQEWNNLYPNNLISSYYDLPDEILYEDEFNEFSGFGH
                     KMFGYPAFTQEDPRSSEKYDDYILLLQIDSVGIGDKEIMWGDSGICNFFITKKDLENK
                     NFSKVLYNWDCY"
     gene            complement(1602..2015)
                     /locus_tag="PLK68_03160"
     CDS             complement(1602..2015)
                     /locus_tag="PLK68_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HRM89443.1"
                     /translation="MVITNIKASFPNDIKTIWNIVTSLDNYTWRSDLSKLEIIKSQEQ
                     FIEYTKNGYPTTFTITCFRPYQRYEFSMENSNMKGHWIGLFSSHNGITSIDFTENITP
                     KKFFMKPFVKRYLKKQQAAYIRDLTAVLEAIEHRF"
     gene            complement(2027..2869)
                     /locus_tag="PLK68_03165"
     CDS             complement(2027..2869)
                     /locus_tag="PLK68_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89444.1"
                     /translation="MEWIERLNTTIEYIENHLTEKIDYEQLAKIAGCPSYHFQKTFLY
                     MTNISLSEYIRKRRISLAAVDLQTGEDKIIDIALKYGYESPTAFNRAFQAVHGIAPSL
                     VRKKNVQIKSFPALKFTFSIQGLDELSFRIEKKDSFKILGISSLLSKELLENFVQIPK
                     QWDKALNDGTLTQLYRLNNQTPLGLLGVNIHQHEDWRYFIAVSSTNHNQNFEEYHIGS
                     ATWAIFSGHGTNRTLQELQRRVITEWLPTSGYEYANIPDIEVYLKADPNDAIYEYWLP
                     IIKK"
     assembly_gap    2975..3000
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3145..3492
                     /locus_tag="PLK68_03170"
     CDS             3145..3492
                     /locus_tag="PLK68_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM89445.1"
                     /translation="MSDIFIKNIPHEQITTLASQVKVQAGQVVSKTLAQNDALSITIF
                     SFDKGEEIGTHGSSGDAMVTVLEGVGKFTVDGQEYVLKRGETLVMPANKPHSVYGQEA
                     FKMLLVVVFPQIK"
     gene            3514..4098
                     /locus_tag="PLK68_03175"
     CDS             3514..4098
                     /locus_tag="PLK68_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM89446.1"
                     /translation="MKKTLYLMRHGQTLFNARKKIQGWCDAPLTELGIKQAKIAGKYF
                     KDNNIVFDHAYSSTSERACDTLELVCDLPYHRVKGLKEWNFGIYEGESEDLNPPLPYG
                     DFFVNYGGEGELQMRQRIADTLLEIMQKEDHKVVLAVSHGGACRGFMRYWQETSQVDQ
                     KGGLKNCCILKFEFEDNEFRLLEIINHNFDETKG"
     gene            4182..5192
                     /locus_tag="PLK68_03180"
     CDS             4182..5192
                     /locus_tag="PLK68_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM89447.1"
                     /translation="METVRLGRTNIVVNRNGFGALPVQRVNMEEAKVILKKAYDNGIT
                     FFDSARAYSDSEEKIGRALSEVRQKIYISTKTMATTVTDFWKDLETSLSLLKTDYIDI
                     YQFHNPSFCPKPGDGSGLYEAMLEAKKQGKIRYIGLTNHRLHVAREAVECGLYDTLQF
                     PFSYLASKKEEELVDLCKEKDVGFICMKALSGGLIDRSDAAYAYLAQFDNTLPIWGIQ
                     KESELDEFIEYNDNPPIMSEEIKAIIAHDQKELSGEFCRGCGYCMPCPMGIEINQCAR
                     MSLMLRRAPSAGWLSEHWQEEMKKIENCINCGKCMSHCPYGLNTPELLKKNYEDYKTF
                     FK"
     gene            5294..>6037
                     /locus_tag="PLK68_03185"
     CDS             5294..>6037
                     /locus_tag="PLK68_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM89448.1"
                     /translation="MREIDRLVEEIRHLQFDDIEKMYQKCLQLKEIALEQKDDYILCL
                     ASNYIIDYYYSCKSQQETVKLANEMLALNEEKGYPDLLMQAYNLYAIAVCNNDYSLAT
                     GFYLKGLKLAEQLNDYIMKAKFNCNLGDVFVNLGQFDLALPYFLESLEQIKKISPKLP
                     EYKIKRFVLCYLIIIYCEKRRLDQAIALMEENKDLFNDTSFDPIDRLWQALKALICYG
                     HGDVAKALEFIDNILGNEIHGFRANEAIYF"
BASE COUNT         2005 a          706 c         1197 g         2103 t
ORIGIN      
        1 ttaagattta atgatgattt tgattttcga agtgaatttg ttggaaagtg gaataaaaag
       61 attcactcat taagagtagc aggaattatt gttgctattt taatgattat ttgtgctatt
      121 ctatgcatga tttatccagt aaaatcagtt actataattg gtacgattgc aaccgggtta
      181 attcttattt taggaattta tcagatcatt gattatatag cggcaccacc attatttcgt
      241 ggtccaggtg gccttgtaag cgctatttgt aatttattaa ttggtctttt gttgatatgt
      301 tcaccaattg aaatgacaat taatacattt gcttttattt ttggatttat tttaatggtt
      361 tatgggctta ataagttgag ttttgctcat caattaagct tttttggaat tgaaagttat
      421 ggttgggtta tatttacggg tataatcaat gttttagcag ctttagcatt tattattgcg
      481 ccattgatgt caaccgtcgt attaaattat attgtagctg cttatttact tgttggtggt
      541 attgctttat tggtcgaagt aattgccatg catgatttaa aattataatt tttagatatt
      601 taaaaagcat ttcggtaatc ggattataaa ttatcgagat gttttttgtt tgcttgtttt
      661 taagaaggtg ctaaaattaa ataaagaaat gataatggaa gctgctgttt tatgttttag
      721 tgaaaggagt gatgatgagt gtttgatttt tttaaaaaga aaaaagatcc agtcgaagag
      781 tcaggaaaac tagaaatgaa taaagaaatg tttttaagta cgatgaaggt gattacagac
      841 tatactcaaa gagatgttat taaaattgaa ttaagtaatg atgagtgtag tatttttgat
      901 agtaaatttg ggggattacc atacttagga gtagatgacg aagtaccatt aagtacagat
      961 aataaacaat tgcgattgct ggggcaaatt aattttgctg atttaccgat gaatagttat
     1021 caattagaag cagggttatt gcaattttgg gctatggata atgatcttta tggtttagat
     1081 tttgagaata tgatatcgca agcagaaagt cgggttattt attattcaag tattgataaa
     1141 actgtatctg aaagtcagat acttaataaa tatcatcctg aaggtgaggg ttattttcca
     1201 atagttgata actttaagct gcattttatt ggtagcgaag aaggaataag tattggtgat
     1261 tatcattttg atggtttatt tacacaagaa tggaataact tatacccaaa taatttaatt
     1321 tcatcatatt atgatttacc ggatgaaatt ttgtatgagg atgaatttaa tgaatttagc
     1381 ggttttgggc ataaaatgtt tggatatcct gcatttactc aagaagaccc ccgcagtagt
     1441 gaaaagtacg atgattatat tttattacta caaattgata gtgtaggtat tggtgataaa
     1501 gagattatgt ggggggatag tggtatttgt aatttcttta taacaaaaaa agacttagag
     1561 aataagaatt tctcaaaagt cttgtataat tgggattgtt attaaaatcg atgttctatt
     1621 gcttctaaga cagctgttaa atcacgaata taagcagctt gctgtttttt taaatatctt
     1681 ttgacaaagg gtttcatgaa gaatttttta ggtgtgatat tttctgtaaa atcaattgaa
     1741 gtaataccat tgtgagatga aaataatcct atccagtgac ctttcatatt actgttttcc
     1801 atactgaatt cataacgctg gtagggtcta aaacaggtaa tcgtaaaagt tgttggataa
     1861 ccatttttag tgtattcgat aaattgctct tgtgatttga taatttccag ttttgaaaga
     1921 tcactgcgcc aagtataatt atctagggaa gtaacaatat tccaaatagt ttttatatcg
     1981 ttaggaaaag aggccttaat atttgtaatt accataacat aactccttat ttttttataa
     2041 ttggtaacca gtattcgtaa atggcatcat ttggatcggc ttttagataa acttctatat
     2101 ctgggatatt agcatattca tatccggaag taggcaacca ctctgtaata acacgacgct
     2161 gaagctcttg aagagttcta tttgttccgt gaccagaaaa gatggcccag gtagcgctgc
     2221 cgatatgata ttcttcaaaa ttttgattat gattagttga actaacagca atgaaataac
     2281 gccagtcttc gtgttgatga atattgactc ctaataatcc gagcggagtc tgattattaa
     2341 gtcgataaag ttgggtaagg gttccatcat ttaatgcttt gtcccattgt ttagggattt
     2401 ggacaaagtt ttctagtaat tctttactta aaagagagct gattcctagg attttaaagc
     2461 tatccttttt ttcaattcta aaacttaatt catcaagacc ttgaattgaa aaggtaaatt
     2521 ttagagctgg gaatgacttg atttgaacat ttttctttct aacaagtgag ggtgcaattc
     2581 catgaactgc ttggaaagca cggttgaatg cggttggtga ttcatatcca tattttaaag
     2641 ctatatcaat gattttgtct tctcctgttt gtaaatcaac tgcagctaaa gaaatacgtc
     2701 gtttgcgaat atattctgat aatgagatat tagtcatgta aaggaaagtt ttttgaaagt
     2761 gataactagg gcagccagct attttagcga gctgctcata atcaattttt tctgttaaat
     2821 gattttcgat atattcaatt gttgtattta gccgttcaat ccattccatt tatattaccc
     2881 tctttattat aacttcttat taatgatcaa tcctcgcttt ttatataaat aaaaagcgag
     2941 attacacttt tttattttat cttgacttag agctnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 attcaggatt taaatgattt agattatctt aataagatag taaactatca ttttaaagtg
     3061 aagcagtatc gttaatcgct ttatttaact tttatgctat aatataaaca tatcttggga
     3121 ataaatattg ataggaggta agatatgagc gatatattta ttaaaaatat tccccatgaa
     3181 caaataacta cattagcatc gcaagtgaaa gttcaagcgg gacaagtagt tagtaagaca
     3241 ctagcacaaa atgatgcttt aagtattact attttttctt ttgataaagg tgaagaaatt
     3301 ggaacgcatg gctctagcgg agatgcgatg gtaaccgtgc tagagggtgt tggaaagttt
     3361 acagttgatg gtcaagaata tgtcttaaag cgtggtgaaa cattagttat gccggctaat
     3421 aaaccgcatt ctgtatatgg tcaagaagca tttaaaatgt tattggttgt ggtttttcca
     3481 caaataaaat agtaaaaata ggagactgat cgggtgaaga agactctata tttaatgcgt
     3541 cacgggcaaa cgctatttaa tgctcgtaaa aaaattcaag ggtggtgtga tgcccctttg
     3601 acagagttag gaattaaaca agctaagata gcaggaaaat attttaaaga caataacatc
     3661 gtttttgatc atgcttatag ttcaacttct gagcgggcgt gtgatacttt agagttagtc
     3721 tgtgatttac cttatcaccg agtcaaagga ctaaaggaat ggaattttgg aatatatgaa
     3781 ggtgaaagtg aagatttaaa tccgccgctg ccatatggtg atttttttgt caattatggc
     3841 ggtgaaggag aattacagat gcgccaaaga attgctgata cattattaga aattatgcag
     3901 aaggaagatc ataaagttgt gcttgcagtt tctcatggtg gggcatgtcg tggttttatg
     3961 agatattggc aggaaactag tcaagttgac caaaaaggcg gcttgaagaa ttgttgtatt
     4021 ttaaaatttg agtttgaaga taatgagttc agattattag aaattattaa tcataatttt
     4081 gatgaaacga aaggttaaaa cctttcgttt tttacttgac ttaaagttta ctcaagggag
     4141 tataataaaa ataggaaatg tcgaattgta ggaggatgaa aatggaaaca gtaagattgg
     4201 ggagaacaaa tattgttgtt aatcgaaatg gttttggggc tttacctgtt caacgagtga
     4261 atatggaaga agcaaaagtt attttaaaga aagcatatga taatggaatt actttttttg
     4321 actctgctcg ggcttatagt gatagtgaag aaaagattgg tagggctctt agtgaggtac
     4381 gccaaaaaat ttatatttca acaaaaacga tggcaacgac agtaacggat ttttggaagg
     4441 atttagaaac aagtttatcg ttattaaaga cagattacat tgatatttat caatttcata
     4501 atccgtcgtt ttgtcctaag ccaggggatg gatcaggttt atatgaagct atgctagagg
     4561 ctaagaaaca aggaaaaatt agatatattg gtttaaccaa tcatcgttta catgtagcaa
     4621 gagaagctgt tgagtgtggt ttatatgata ctttacaatt tccttttagt tatttggctt
     4681 caaaaaagga agaggaacta gttgatttat gtaaagaaaa agatgttggt tttatttgta
     4741 tgaaggcttt gtcaggtggt ttaatagacc gttctgatgc ggcatatgcg tatttagcgc
     4801 agtttgataa tacattgccg atttggggaa ttcaaaaaga atcagagtta gatgaattta
     4861 ttgagtataa tgataatcca ccaattatga gtgaagaaat caaagcgata attgctcatg
     4921 atcaaaaaga gttatctggt gaattttgtc ggggttgcgg atattgtatg ccatgtccaa
     4981 tgggaataga aattaatcag tgtgctcgaa tgtctttgat gttacgacga gctcctagtg
     5041 caggctggtt gtcagaacat tggcaagaag aaatgaaaaa aatagaaaat tgtattaatt
     5101 gtggcaaatg tatgtcgcat tgtccgtatg gattaaatac acctgaattg ttgaaaaaaa
     5161 attatgaaga ttataaaaca ttttttaaat aattagatat acgattccta gttaaaacta
     5221 ggaatttttt aaccaatatt agaactattt tatgttataa tgaaatcaaa gtgcttaaag
     5281 aaaggagaaa ttaatgcgtg agatagatcg tttagtcgaa gaaatccgtc atttacagtt
     5341 tgatgatatc gagaaaatgt atcaaaaatg tttgcagtta aaagaaatag cactagaaca
     5401 aaaagatgat tacatattat gtttagctag taattacata atcgattact attattcttg
     5461 taaatcgcaa caagaaacag ttaaattagc gaatgagatg ttggctctaa atgaagaaaa
     5521 agggtatcca gatttattga tgcaggctta caacctatat gctattgcag tatgtaataa
     5581 tgattattct cttgctactg gtttttatct caaaggattg aagttagctg agcagttaaa
     5641 tgactatata atgaaagcaa aatttaactg taatttagga gatgtttttg ttaatttagg
     5701 tcaatttgat ttagcgttac cttatttttt agaatcgctt gaacagatca aaaagatttc
     5761 tcctaaactg ccggaatata aaataaaacg ttttgtgtta tgttatctta ttataattta
     5821 ttgtgaaaaa agaagattgg atcaagcgat tgctctaatg gaagaaaata aggatttatt
     5881 taatgatact tcatttgatc cgattgatcg gttatggcaa gcattaaaag cattaatttg
     5941 ctatgggcat ggtgatgtag caaaagcttt agagtttatt gataatatat tagggaatga
     6001 gattcatggt tttcgtgcaa atgaagcaat ttatttt
//
LOCUS       DAOMTA010000074         6016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_244_length_6016_cov_2.467203, whole genome shotgun sequence.
ACCESSION   DAOMTA010000074 DAOMTA010000000
VERSION     DAOMTA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244_length_6016_cov_2.467203"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1011
                     /locus_tag="PLK68_03190"
     CDS             13..1011
                     /locus_tag="PLK68_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM89449.1"
                     /translation="MATIKDVAELANVSSATVSRILNNDKTLSVPEETRQAVFNAAKE
                     LNYIKKRKATKNEFTIGIVQWYSLQQEIDDPYYLQIRQGIENFCLQNNINVIRTFRAD
                     QNYLDVLKGVDGLVCVGKFNNNEISQFKKISNKVIFLDMHSPNSDDSTITLDFHKAVI
                     DALDYLKSLNHHKIGYLGGKEYLEDNVVYQDARKEIFIDYCNKNKIIYKKYLLEGEFT
                     TESGYSMMMDLINQKDLPSAIFCASDPIAIGALRALQEHNLSVPRDISLVGFDDIKAA
                     SFTNPPLTTIYAPASLMGEYGASMVYNILSKYNSPTPMRITLPCTLIERESCKKVD"
     gene            1215..1646
                     /locus_tag="PLK68_03195"
     CDS             1215..1646
                     /locus_tag="PLK68_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HRM89450.1"
                     /translation="MDKQLNKLLADLVVEYHKLQSYHWYIKGKDFFTVHAKLEELYNG
                     VNKAIDEVAEAILMTGFKPAASMQEFLDISAIEEVKGEHIASKDIYKVVLNDFNYLLD
                     RIKSLKNNADNENNYLISSLMDDYIKEFTKSIWMISQVVEN"
     gene            1667..2230
                     /gene="ahpC"
                     /locus_tag="PLK68_03200"
     CDS             1667..2230
                     /gene="ahpC"
                     /locus_tag="PLK68_03200"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HRM89451.1"
                     /translation="MSLIGKEITEFEAQAFHENEFKTIKKEDLLGKWNVLFFYPADFT
                     FVCPTELEDLANKYEEFKNINCEIYSVSCDTHFVHKAWHDTSKTIKKINYPMLADPTA
                     KLAKDLDVYIEADGLAERGSFIINPEGKVVMYEVIAGNVGRNADELFRRVQASQFVYE
                     HGDEVCPAKWKPGEETLKPSLDLVGLI"
     gene            2232..3839
                     /locus_tag="PLK68_03205"
     CDS             2232..3839
                     /locus_tag="PLK68_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89452.1"
                     /translation="MSNLYDAIIVGGGPAGLSAAIYLARAKFSVLVIEKEKIGGQITI
                     TSEVVNYPGILQTSGQELTEAMRQQAQNFGAEFLLVTVKGINLKEPVKKIITDKGEFS
                     SIGIVLATGSHPRKLGFPGEKEFQGHGVAYCATCDGEFFTGKDIFVIGGGFAAAEEAV
                     FLTKYARKVTIIVREEDFTCAKQVSDKAKNHPNIEVHYNTEIIEAKGNHQLEQATFKN
                     NATGETWSYHAEANNTFGIFVFAGYEPATEIFKNQVELNNYGNLIVDQNQKTNLDGVY
                     GAGDVCVKELRQVVTAVSDGATAATSLEKYIPNIVTALKLSPKKIKIKEAVQTSKETS
                     ENDDSFITPKIKQQLAPIFAKLEKQLIIKCSLDHSKLAGEIKSFIEEFTTLSDKLSYQ
                     FEESNSSPAMRFYDENNNYLNISYHAVPGGHEFNSFVIAIYNAVGPKQPLDTQIINEI
                     KQINKPTNIKVVVSLSCTMCPDVVMASQRIAIENNNVDAHVFDLAHFPELKEQYQIMS
                     VPCMIINDQDVYFGKKDIQQVIEIIKK"
     gene            complement(3857..5854)
                     /locus_tag="PLK68_03210"
     CDS             complement(3857..5854)
                     /locus_tag="PLK68_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM89453.1"
                     /translation="MLLKDRRKLIIIGVVAVALIIGAVFFIFSRGKSADDYVKEYYGY
                     LENKEYGKMYNMLTKGSLAKTSQKVFEARYKNIYEGIEAKNIEIKIGEVEDGIVNYTL
                     TMDTLAGKISSENKVEVKDGELVYNEAMILEGLKEDYKIKINTDSASRGKILDRNGKE
                     LATKGEAYSVGLVQGKLNGEADYEAIGKIIGMSRDEIKKVMSASWIKDDSFVPLKEMA
                     KDEEGKAKAQQLLTIKGVKLNTVSVRYYPYGEAASHVTGYLQQVNAEDLKKHKNEGYS
                     ETSLIGRSGIEAAYEADLKGTDGKEIIIIDKNNKLVETLATKKVENGKDIKLTIDADL
                     QQSLYKEYQNDKSASVAMNPKTGEILALVSTPSFSSNDFIFGFSSAEWQALNDDANKP
                     LTNRFRGTYIPGSSMKPITAAIGLESNKIDPDEDFGAKDKWQKDSSWGNYYVTTLHAP
                     KPNNLNNAIIYSDNVYFARAASEIGKEKLIEGYEKLKIGSKIPFELSLNASQYQNKDS
                     KFDDQQLADSGYGQGQLLLNPVQLASIYGAFVNEGTIAQPYLVIDEKPNDAWIKDVFS
                     KDTVKRIKEALVGVISDSNGTGHSIYHQDIELAGKTGTAELKSSQNDTSGSEIGWFTV
                     MTTNSDNPVLITTMVEDVKNRGGSGYVVDHMKAPLGSYLYR"
BASE COUNT         2097 a         1027 c          883 g         2009 t
ORIGIN      
        1 aggagggtaa tcatggctac aattaaagat gttgcagagc tagcaaatgt ttccagtgcc
       61 actgtttcaa gaattttaaa caatgataaa acattatctg ttcctgaaga gacaaggcag
      121 gcggtattta atgcagctaa agaattaaac tatataaaga aaagaaaggc aaccaaaaat
      181 gaattcacga ttggaattgt tcaatggtat tcattgcaac aagaaattga tgacccttat
      241 tatttacaaa taagacaagg tatcgagaac ttttgcttac aaaataatat aaatgttatt
      301 agaactttta gagccgatca aaactatcta gatgttttga aaggtgtaga tggcctagtt
      361 tgtgttggta agtttaataa taatgaaatt tctcaattta aaaagatttc aaataaagtt
      421 atctttctag atatgcatag tccaaatagt gatgacagta cgatcacttt agacttccac
      481 aaagcggtta ttgatgcttt agattattta aaatctttaa atcatcataa gatcggttat
      541 ttaggcggta aagaatatct tgaagacaat gttgtatatc aagatgctcg taaagaaata
      601 tttattgatt attgtaacaa aaataagatt atttacaaaa aatatttact tgaaggtgaa
      661 tttacaactg aatctggtta ttccatgatg atggatctga tcaatcaaaa agatttacca
      721 tcagcgattt tttgtgccag cgatccaatc gcaattgggg ctcttagagc tcttcaagaa
      781 cataatctca gtgttccaag agatatttca ctagttggtt ttgatgatat taaggcagcc
      841 agcttcacta atccaccact gacaacaatt tatgctccag catcacttat gggggaatat
      901 ggagcaagta tggtatataa tattttaagc aaatataatt ctcctactcc aatgagaata
      961 acactcccat gtactttaat tgaacgagag tcttgtaaaa aagtagatta aatctacttt
     1021 ttttatttat taatttattc tctctaaata atacaataaa atactaattt ggcataaata
     1081 aaaaataaag gtgcataata tttgtatcca tcatcattcc atgttaagat ttatacagtt
     1141 agcaagagct aacctaaata actataaatc tttgtcgaca aaaggtaata aaaatagtaa
     1201 aggagtgaaa cacaatggat aaacaattaa ataaactatt agcagattta gtagttgaat
     1261 accacaaatt acaaagttat cactggtata ttaaaggaaa agatttcttt actgtccatg
     1321 caaaattaga agaactatat aacggagtta ataaagccat tgatgaagtt gcggaagcta
     1381 tcttgatgac tggttttaaa ccagcagcat caatgcaaga attcttagat atttctgcaa
     1441 ttgaagaagt caaaggtgaa catattgcat caaaagatat ctataaagta gttctaaatg
     1501 attttaatta tttattagat aggattaaat cattaaaaaa taatgcagat aatgaaaata
     1561 attatttaat ttcatcttta atggatgatt atattaaaga attcacaaaa tctatttgga
     1621 tgataagcca agtagttgaa aattaataaa aaaaaggaga aaaacaatgt cattaatcgg
     1681 aaaagaaata acagaatttg aggctcaagc ctttcatgaa aatgaattta aaacaatcaa
     1741 aaaagaagat ttattaggaa aatggaatgt cttattcttc tatcctgcag attttacatt
     1801 tgtctgccca actgaattag aggatttggc aaataaatac gaagaattta aaaatataaa
     1861 ttgtgaaatt tattcagtat catgtgatac tcatttcgtt cataaagctt ggcacgatac
     1921 atctaaaaca attaagaaaa tcaattatcc aatgttagct gatcctactg ctaaattagc
     1981 aaaagatcta gatgtatata ttgaagctga tggtttagca gaacgtggaa gtttcatcat
     2041 taatccagaa ggaaaagttg ttatgtatga agtaatcgct ggaaacgttg gacgtaatgc
     2101 cgatgaatta tttagacgtg ttcaagcttc acaatttgtt tatgagcacg gtgatgaagt
     2161 atgtccagca aaatggaaac caggagaaga aacattaaaa ccatcactag atctagtagg
     2221 attaatctaa aatgtctaat ctttatgatg ctattatcgt tggcggtggt cctgctggac
     2281 tgtccgctgc gatatatcta gctagagcta aattttcagt tcttgtaatt gaaaaagaaa
     2341 aaatcggtgg tcaaataaca attacttctg aagttgttaa ttatcctgga attttacaaa
     2401 ctagcggtca agagctaaca gaagcaatgc gacaacaggc acaaaatttt ggtgcagaat
     2461 tcctacttgt aacggttaaa gggatcaatt tgaaagaacc agtaaaaaaa atcatcactg
     2521 ataaaggtga attttcaagt attgggattg ttttagcaac aggttctcat ccacgtaaac
     2581 taggttttcc aggcgaaaaa gaatttcaag gtcatggtgt agcctattgt gctacttgtg
     2641 atggtgagtt ctttaccggt aaagatattt ttgtcattgg cggtggcttt gctgctgcag
     2701 aagaagccgt gtttttaacc aaatatgctc gtaaagttac aatcattgtc cgtgaggaag
     2761 atttcacttg tgctaaacaa gttagcgata aagctaaaaa tcatccaaat atcgaagttc
     2821 attataacac ggaaataatt gaggctaaag gtaatcacca gttagaacaa gcaacgttta
     2881 aaaacaacgc aactggtgaa acttggtcat atcatgccga agcaaataac acatttggaa
     2941 tctttgtatt tgcaggttat gagccagcaa ctgaaatatt caaaaatcaa gttgaattaa
     3001 ataactatgg aaacttaatt gtcgatcaaa atcaaaaaac taatcttgat ggtgtttatg
     3061 gagctggtga cgtttgtgtc aaagaattac gtcaggttgt cactgcagtc agtgatggcg
     3121 ctactgctgc gacctcatta gaaaagtata tccctaatat tgttactgct ttaaaacttt
     3181 cccccaagaa aataaagatt aaagaagcag tacaaacaag caaagaaaca agtgaaaatg
     3241 atgatagttt tattaccccc aaaataaaac aacagttggc tccaattttt gctaaattag
     3301 aaaaacaatt aataattaaa tgctccctcg atcatagtaa acttgctggt gaaatcaaat
     3361 catttattga agaatttact actctcagtg acaaactttc ctatcagttc gaagaatcaa
     3421 actcttcacc agcaatgcgt ttctatgatg aaaataataa ttatttaaat attagttatc
     3481 atgctgttcc tggtggtcat gaatttaatt cctttgtaat tgcaatctat aatgcagttg
     3541 gtcctaaaca accgcttgat acgcaaatca tcaatgaaat taaacaaatt aacaaaccaa
     3601 ccaacattaa ggtagttgtc tccctttctt gtactatgtg cccagatgta gtaatggcaa
     3661 gccaaagaat tgcaattgaa aataataatg ttgatgccca tgtatttgat ttggcacatt
     3721 tccccgaact taaagaacaa tatcaaatta tgtcagttcc ttgtatgatc ataaatgatc
     3781 aagatgtgta ttttggtaaa aaagatatcc agcaagttat tgaaattata aaaaagtaag
     3841 cttacgctta ctttttttaa cgatataaat aactacctaa cggtgctttc atatggtcta
     3901 ctacataacc gcttcctcca cgatttttta catcttcaac catcgttgtg attaaaaccg
     3961 gattatcact atttgttgtc attactgtaa accagccaat ctcactacca cttgtatcat
     4021 tttgtgaaga ttttaattct gctgtcccag tcttaccggc taattcgata tcttgatgat
     4081 aaatcgaatg cccagttcca ttactatcac tgatcactcc tactaaagcc tctttgattc
     4141 tcttaactgt atctttgcta aaaacatctt tgatccatgc atcattaggt ttttcatcaa
     4201 taactaaata aggctgagca atcgtcccct cattaacaaa cgctccatat attgatgcta
     4261 actgtacagg gtttaataat aattgacctt gcccgtagcc actgtcagct aattgttgat
     4321 catcaaattt actatcttta ttttgatatt gagaagcatt taaagataat tcaaaaggta
     4381 tttttgatcc aatctttaat ttctcatatc cctcaatcaa cttttccttt cctatttcac
     4441 tagcagctcg agcaaaataa acattatcag aatagataat tgcattattc aaattattag
     4501 gctttggagc atgaagtgtt gttacataat aattacccca actagaatct ttctgccatt
     4561 tatccttagc tccaaaatct tcatctggat ctatcttatt actttctaaa ccaattgctg
     4621 cagtaattgg cttcatacta gagccaggaa tatatgtccc tctaaaacga tttgttaaag
     4681 gtttattagc atcatcattt aatgcttgcc attcagcact actaaaacca aaaataaaat
     4741 cattacttga aaacgacggt gttgatacta aggctaaaat ctcacctgtc tttggattca
     4801 tagctaccga agcactctta tcattttgat attctttata caaagattgt tgcaaatcag
     4861 catcaatcgt taattttata tctttaccat tttcaacttt tttagtagct aatgtttcta
     4921 ctaatttatt atttttatca ataattataa tttctttacc atcagttcct tttaaatctg
     4981 cttcataagc agcctcaatt ccacttcgcc caattaaaga agtttcacta tagccttcat
     5041 ttttatgttt cttcaaatcc tcagcattta cttgctgcaa atatcctgta acatgtgaag
     5101 cagcttctcc ataaggataa taacgaacac ttacagtatt tagcttaact cctttgattg
     5161 ttaatagttg ttgagcttta gcttttcctt cttcatcttt tgccatctct tttaacggta
     5221 cgaacgaatc atctttgatc catgaagcac tcattacttt ttttatttca tcacgactca
     5281 ttccaattat tttgccaatt gcttcataat cagcttctcc atttagtttg ccttggacta
     5341 aaccaacaga ataagcctcc cctttagttg caagttcttt accatttcga tccaaaattt
     5401 ttccccgact agcactatca gtattaattt ttatcttata atcttctttt aatccttcca
     5461 agatcatcgc ttcattataa acaagctccc catcttttac ttcaacttta ttttcagaac
     5521 ttatttttcc tgccaaagta tccatcgtta aagtataatt aacaatccca tcttcaacct
     5581 cgccaatttt aatttcaata tttttagctt caatcccctc ataaatattt ttatatctag
     5641 cctcaaatac tttttgactc gttttagcaa gtgagccctt tgttaacata ttatacattt
     5701 ttccatattc tttattttct aaataaccat aatattcttt tacataatca tcagcacttt
     5761 taccccgtga aaaaataaaa aaaactgcac ctataattaa tgcaactgcc acaacaccaa
     5821 taatgattaa ttttctccga tctttcaata acatacattt ccctctttcc tagctaatta
     5881 gtattatcta gataatattt tagcataatt ttgataacta aacagcattg tttataattt
     5941 aataattata tcagcattca ataaacaatt cgcaaataca tcatacgtct tatgtgatag
     6001 tgtgctataa taagat
//
LOCUS       DAOMTA010000075         6003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_245_length_6003_cov_2.266980, whole genome shotgun sequence.
ACCESSION   DAOMTA010000075 DAOMTA010000000
VERSION     DAOMTA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_6003_cov_2.266980"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLK68_03215"
     CDS             <1..985
                     /locus_tag="PLK68_03215"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRM89454.1"
                     /translation="RMSCTSVLNNLDANKYEITLVGIDLDGGWHYLDAKQTDLAKNTW
                     LDNSSMVEDVYGLLKNQDVAFPVLHGMYGEDGTIQGLFELAKLPYVGCRVMGSSVAMD
                     KIYTKKILDTVGVPQVKSVYVKKRYDEKLVVVTNTFDEIEEIEDYIVRELGMPCFIKA
                     SRSGSSVGCYRCDNQTELIGKLSEAAKYDRHVVVEECIDCIELETAVLGNDDVIVSRV
                     GQIMPHGEFYTFESKYEDEESKTCIPALVDEQIQEQIRQYAIKVFKAVDGHGLSRVDF
                     FLDKKTNKIYLNEINTMPGFTKISMYPQLMNDFGITYPELLDRLIVLALQK"
     gene            1167..1943
                     /locus_tag="PLK68_03220"
     CDS             1167..1943
                     /locus_tag="PLK68_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89455.1"
                     /translation="MNEDYLTIGELAQKMDVTVRTLQYYDREGLLKPAAISKGGRRLY
                     STKDIVKLHQILSFKYLGFSLVEIKTKLFNLDTPQEVAAILNQQKSVIQEQIANLSEA
                     LEATIALSREVQEMNTVDFKKYAEIIELLRLGNKEYWVWKHFDNTITDHIKERFGDDP
                     EAGLRIFSTYQEVLDKLYVLKKQGVSPDSPECFMIAKQWWEMILEFTGGNLELLPELQ
                     KFNDKKNDWNNDLAVKQKEVDNYLTAALEYYFKRIQKEQE"
     gene            1945..2796
                     /locus_tag="PLK68_03225"
     CDS             1945..2796
                     /locus_tag="PLK68_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89456.1"
                     /translation="MDIALQINNLGKAYGKHQVLENISFTVVKGEIFALLGTNGAGKT
                     TTLECLEGIRRYDTGKILINGKLGVQLQSSSLPADITAKEAIMLFAKWQDLEVTDDYF
                     IYLGIKAFLKKQYHQLSTGQKRRLHLAIALLGHPDIIVLDEPTAGLDVEGRNSIHQEI
                     KRLKTQGKTILLASHDMTEVEELCDRIGVLNHGKIVFIGAPDQLHQTMQSKFKLKVRF
                     SKVPRLNEQFEIVSQEQEYYIFETTNLEITLKAIIQLTEEQSIKIMEINTVQPKLEER
                     FLKEVQS"
     gene            2793..3512
                     /locus_tag="PLK68_03230"
     CDS             2793..3512
                     /locus_tag="PLK68_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM89457.1"
                     /translation="MRAFIYQIGLNWKLNLRSKELLVHYYVVPLVFYLFIGGVFISIL
                     PDANKTIIQVMSVFAITMGGVLGSPYPLVEFYHSDIKKAYQVGKIPLWTIAASNFISG
                     IMHLFVMSLIILISAPIIFKAQIPENIFIYLLGAILFIIVSLSIGMVFGVFFKSAAKM
                     GMATQLVFLPSIMLSGIMFPVAMLPNVLQVIGRILPATWGFELMCTNDFLWLNVAVQL
                     IIFMIMLIVASFKIKQIRKED"
     gene            complement(3897..4709)
                     /locus_tag="PLK68_03235"
     CDS             complement(3897..4709)
                     /locus_tag="PLK68_03235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRM89458.1"
                     /translation="MSKKKLKLKRPIKIFLNFLLLLSLVTGTYLFINRKETSIKSPNK
                     STSTKRPRIVNASFIGDLLYEQPYYDWIGTSYNDKGYYDLVKPYFLNDDLTLANMEVP
                     IGGKELGVSGTGYSFNAVNLANNHANDAGPQGRINTLSFFKKQQILTTGIYESKEVRT
                     QIPTKTINDITFSFLGYTYKTNKPDNQNRELIGYYRNLDTMKLDNAHKEIIKQEVAQA
                     KQLSDIVIVSVHWGNEFTYAVNSEQKELANYLNELGVDVIIGHHSHCIQPIE"
     gene            4869..5492
                     /locus_tag="PLK68_03240"
     CDS             4869..5492
                     /locus_tag="PLK68_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRM89459.1"
                     /translation="MTNEIKLEVTCVHCLGVGEKQLYPIINRQDLEAKQNIFEEDLFL
                     YRCPHCGHYQRISYECMYYDEKLKYAVVLSHDGHRFLKKVKIDLTAYQLRFVKSVSEL
                     KEKIVIKENGLDDRIVEIMKHNIQTTLRSKASYQLVLRDGQGLEFVLLYPDNEVIKIF
                     MFNKQDYLTLTKKYNRCLANDYIVDRLFASRTVLRLNKFNNLKLNCH"
     misc_binding    5657..5908
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2036 a          738 c         1185 g         2044 t
ORIGIN      
        1 aagaatgtct tgtacatcgg tattaaacaa tttggatgcg aataaatacg aaatcacctt
       61 agttgggatt gatttagatg gtggttggca ttatcttgat gcgaagcaga cagatttagc
      121 taaaaatact tggttagata acagtagcat ggttgaagat gtttatggat tattaaaaaa
      181 tcaagatgta gcatttccag tattacatgg aatgtatggt gaagacggaa caattcaagg
      241 tttatttgaa ttggcaaaat taccttatgt tggctgtcgg gtgatgggct ctagtgtagc
      301 aatggataaa atctatacaa aaaagatttt agatacagta ggtgttccgc aagttaaatc
      361 agtatatgtg aaaaaacgtt atgacgaaaa attagttgta gtaacaaata cttttgatga
      421 gattgaagaa attgaagact atattgttag agaattagga atgccttgct ttattaaggc
      481 tagtcgttca ggttcaagtg ttggatgtta tcgttgtgat aatcagactg aattgattgg
      541 gaaattaagt gaggcagcta aatatgatcg tcatgtcgta gttgaggagt gcatagactg
      601 tattgagtta gaaacagctg tattaggtaa tgatgatgta attgtttcac gagtcggtca
      661 aatcatgcct catggtgagt tttatacgtt tgaatcgaaa tatgaagatg aagaaagtaa
      721 aacgtgtatt ccggctctgg tagatgagca aatccaagaa caaattcgtc aatatgcaat
      781 taaagttttt aaagctgttg atggtcatgg cttaagccga gttgattttt tcttagataa
      841 aaaaactaat aaaatttatt tgaatgaaat taatacaatg ccaggattta ctaagatttc
      901 aatgtatcca caattaatga atgatttcgg gatcacttat ccggaattat tggatcgctt
      961 gattgtcttg gccttacaaa aataagaact tgttttgaac tgaacttccc aagtaggaca
     1021 gtttataaat agtttctaat tagaggattg tcacctgtaa ttcacaggtg acaatccttt
     1081 taatttagtt cttttttata aataatttca ataattgttg ctcgtgacgt aaggtcatga
     1141 actataatgt ttatagaggt gatgatatga atgaggacta cttaacaatt ggagaattag
     1201 cacaaaagat ggatgtaaca gttcgtacac tacaatatta tgatcgtgag gggttgttga
     1261 aaccagcagc aattagcaaa gggggaagac ggctctattc aactaaagat attgttaaat
     1321 tgcatcaaat tttatcattt aaatatcttg gtttttcact tgtagaaatt aaaactaaat
     1381 tatttaatct cgacacacca caggaagttg cagcgatttt aaatcaacaa aaaagtgtga
     1441 ttcaagaaca aattgctaac ttatcagaag ctttagaagc aacgattgcc ttatctagag
     1501 aagttcaaga aatgaatacg gtagatttta aaaaatatgc tgaaataata gagttacttc
     1561 gtttgggaaa taaagaatat tgggtgtgga aacattttga taatacaatt actgatcata
     1621 ttaaagaacg atttggtgat gatccagagg ccgggttaag aatatttagc acttatcaag
     1681 aagtcttaga taagctgtat gtgttaaaga aacagggggt aagtcctgat agtccagaat
     1741 gctttatgat tgccaaacag tggtgggaaa tgattttaga atttactgga ggtaatttag
     1801 aactgttgcc agagttacaa aaatttaatg ataaaaagaa cgattggaat aatgatctgg
     1861 cagttaaaca aaaagaagta gataattatc taactgcggc tctagaatat tattttaaga
     1921 ggatccaaaa ggagcaagag taatatggat attgcattgc agattaataa tcttggtaag
     1981 gcttatggta aacatcaggt tttagaaaat attagtttta ctgttgttaa aggggaaata
     2041 tttgctttac ttggcacaaa cggtgcgggg aaaacaacga cattagaatg tttagagggg
     2101 attcgacgat acgatacagg taaaatttta ataaatggta agctgggggt tcaactgcaa
     2161 tcatcatcat taccagcgga tattaccgct aaagaagcaa tcatgttatt tgcaaaatgg
     2221 caagatcttg aggtgactga cgattatttt atttatttag gaattaaagc ttttctaaaa
     2281 aagcaatatc accagttatc gacaggacaa aagcgtcgtt tacatcttgc aattgcgcta
     2341 ttaggacacc ccgatattat tgtacttgat gagccaactg ctggacttga tgttgaggga
     2401 cgtaacagta tccaccaaga aattaaacga ctaaaaaccc aaggtaaaac aattttatta
     2461 gcgagtcatg atatgaccga ggttgaagag ttgtgtgatc gaatcggagt attaaatcat
     2521 gggaaaattg tttttattgg agctcctgat caattgcatc aaacaatgca aagtaaattt
     2581 aaattaaagg ttcgtttttc aaaggtgccc cgattaaatg agcagtttga aatagtttct
     2641 caggaacagg agtattatat ctttgagact acaaacttag agataacatt aaaagcaatt
     2701 attcaattaa ccgaggaaca aagtattaaa attatggaaa taaatactgt tcagccaaag
     2761 ctggaagaaa gatttttaaa agaggtgcaa tcatgagggc tttcatctat caaattggat
     2821 taaactggaa attaaatctt cgcagtaaag aactattagt tcattattat gtggttccct
     2881 tagtctttta tttgtttatt gggggtgttt ttatatcgat tttgcccgat gctaataaaa
     2941 cgataattca agtaatgagt gtttttgcta ttaccatggg tggagtctta ggttctccat
     3001 atccgttagt ggaattttat catagtgaca taaaaaaggc ttatcaggta ggaaagattc
     3061 ctttatggac gattgccgca agtaatttta tttctgggat tatgcattta tttgtgatga
     3121 gtttgataat tttaataagt gccccgatta tttttaaagc acaaatacct gaaaatattt
     3181 ttatatattt attgggggcg attttattta tcatagttag tttgagtatt gggatggttt
     3241 ttggtgtatt tttcaaaagt gcagcgaaaa tgggaatggc aacgcaacta gtttttttac
     3301 cgtcgatcat gttatcagga ataatgttcc ctgtagcaat gctccctaac gtattacagg
     3361 tgataggcag aattttaccg gccacatggg gatttgaatt aatgtgtaca aatgattttt
     3421 tgtggcttaa tgtggctgtt cagttaataa tttttatgat aatgctaatt gtagcaagtt
     3481 tcaagatcaa gcagataaga aaagaagatt aaaaaagcct aaattagtac aagctgattt
     3541 agtgcttttt attgtttaaa ctgttgatca atcacattgt taaaactatt ggtaatgaag
     3601 tctttagtta atccttttga ataacgatta tgactttttt ctaatttttc attatattta
     3661 tctataggca ccaatttaaa attttcaaat ttttgatcat aatagttgat gactgggaga
     3721 gaattagtat ttttaattat ggtagaatta ttattctttt caaaagttat ttgcaggatc
     3781 attgaaacat tatatgcatt ggcaaattct ggtgttgctc tagttacctg attatcagct
     3841 gaaataaatg atcctaatga atatacaaca agtgttttat ggttggtagt ttctaactat
     3901 tcaattggct gaatacaatg tgaatgatga ccgataatga catcaacccc taattcgttt
     3961 agataattag ctaattcttt ttgttcacta ttgacagcat atgtaaactc attgccccaa
     4021 tgaacactaa cgataactat atcactaagc tgttttgctt gtgcaacttc ttgttttatg
     4081 atttctttat gagcgttatc gagtttcata gtgtcaaggt tgcgataata accaatcagt
     4141 tctctgtttt gattatcagg cttattagtt ttataagtgt agccaagaaa actgaaggtt
     4201 atatcattta tggtttttgt agggatttgt gttcggactt cttttgattc atagattccg
     4261 gttgttagaa tctgttgttt tttaaagaag cttaatgtat tgatacgtcc ttgaggaccc
     4321 gcgtcgttag catgattatt agcaagatta acagcattaa aactataacc agttccggat
     4381 actcctaatt ctttgccgcc gatagggact tccatattgg ctaatgttag atcgtcattt
     4441 aaaaaatatg gttttactaa gtcataatag cctttatcat tgtaacttgt tccaatccaa
     4501 tcatagtatg gctgttcata taataaatcg ccgataaaag atgcattaac tattcttggc
     4561 cttttagtag aagtggattt gtttggtgat ttgattgatg tttcttttct attgataaat
     4621 aaatacgtcc ccgttacgag ggataaaagc aggagaaaat taagaaatat tttaattgga
     4681 cgttttagtt ttaatttttt ctttgacatt tttatcaccc tttatattgt aacatgatca
     4741 atcatgctaa ttgtcttaaa taagctgaaa aatctttgca tattcttaag ataaattata
     4801 gaagatttgg tagttagatt tatttagttt agtagaatgt atgcaaggac attgaggagg
     4861 aatgaaagat gactaatgag attaagctgg aagttacctg tgttcattgc ttaggggttg
     4921 gggaaaaaca gttatatcca ataattaata gacaagattt agaggcaaaa cagaatattt
     4981 ttgaagaaga tttgttttta tatcgttgtc cgcattgtgg acattatcaa aggatttcgt
     5041 atgaatgtat gtattatgat gagaaattaa aatatgcggt ggtgttgagc catgatggac
     5101 accgtttttt aaagaaagtg aaaatagatt taaccgctta tcaacttcgt tttgtaaaaa
     5161 gtgtaagtga attaaaagaa aaaatcgtta ttaaagaaaa tggtttagat gatcgaattg
     5221 ttgagataat gaaacataat attcaaacta ctttaaggag caaagcgtct tatcaattgg
     5281 tattaagaga tggacaagga ctagagtttg ttttattata tccagataat gaagttatta
     5341 agatatttat gtttaataag caagattatt tgactttaac aaagaagtat aatcgttgtt
     5401 tggctaatga ttatatagta gataggctct ttgcttctag aacggtttta cgtctaaata
     5461 agttcaataa tttgaaacta aattgtcatt agaataaata gatgatattg gtaaaatact
     5521 tgataaaatc aggactttta tattgaatcg acttgtgaaa taattaatta ttaatcagat
     5581 aattaattat ctataaaatt tatgaaaaag ggttgacttc gacaaaatta cgggctatta
     5641 tattcagtaa cataacttaa atactttgat aggaactagt aaataaattt atcgagcata
     5701 gagagattct gggtggtgaa aagaattgtt gatgatttgt tgaactcatc cttgagtgga
     5761 gcgctgaatc aagtaggtgg ctacggagtg caaccgttat ctagcaaaag tatgaactat
     5821 atataatagc gtacttggtg agattaatat cgtgaggtat taataaattg aggtggaacc
     5881 acggggttac tatactctcg tcctcaaaat gacatagtct gtcattttga gtgcgagagt
     5941 ttttttataa gtatttatta tgcaaaaggg aggaagaaaa aatgatgaat tatcaaaagt
     6001 ata
//
LOCUS       DAOMTA010000076         5996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_246_length_5996_cov_2.518600, whole genome shotgun sequence.
ACCESSION   DAOMTA010000076 DAOMTA010000000
VERSION     DAOMTA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_5996_cov_2.518600"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLK68_03245"
     CDS             complement(<1..337)
                     /locus_tag="PLK68_03245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM89460.1"
                     /translation="MIETSVIMATYKEEQSFLKQSIESILNQSYKDFEFIIILDNPEN
                     SEHIKLINKYAKIDNRIRFFINEKNMGLTKTLNKGLTLTKGRYICRMDADDISFNNRL
                     EVQKEYLESN"
     gene            complement(421..1305)
                     /locus_tag="PLK68_03250"
     CDS             complement(421..1305)
                     /locus_tag="PLK68_03250"
                     /inference="COORDINATES: protein motif:HMM:NF020339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GT-D fold domain-containing glycosyltransferase"
                     /protein_id="HRM89461.1"
                     /translation="MVKIIKNVIKNIIYLFKYSIEDREKETLYEALIRVEDVYKYEIM
                     DNLESAAKLDVISAEETIDILLHKPKSFCRFGDGEILLMQGQSIAFQKFDRILSEKMF
                     EILKSNSDKCYVGINYNYFHSTKNLNEFNRRFYMVNAKQYREFLLNYCNNDRKYIAAG
                     FNQMYMIYNIDLDDYYKKLKSLFKDKEIVLFVGKNVLDNIQFDIFELAKSKEIVYGPS
                     KNAFDSYDELFSKSKTYSKDKTLCFILGPTSKVLVYELSKLGYTAWDIGHMAKDYNAY
                     KLKIEKTKDNVVDFYKPD"
     gene            complement(1323..2687)
                     /locus_tag="PLK68_03255"
     CDS             complement(1323..2687)
                     /locus_tag="PLK68_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRM89462.1"
                     /translation="MNRKIMPIMLFMIEVVMYYFICLYFHMDIDLIVGSGTLYFLFLF
                     IYGHYSLNTCLIWNEIQQLVKSSFCFYIALLVLVPQSTGYERKMHLTLMAISMFLISL
                     LASRFLRIAFREQFARKTLVIGTGYEAARLGKISNNNRFALTNIMGYVDVNKTNELFR
                     FEQENIIKHSPIYEYDKIDEAIENLGIEQIIIAIPEADQQVIDKVMSDIYGKVDSVKY
                     LPDVNGTMTFSSEVQDFDGQLLIATSNDEIGLLDKFIKRLIDILAGIAGVMTLLPLMA
                     YVKYKYVKSGDHDNIMFSQERIGKNGKLIKIYKFRSMIPNAEAELERMMEEDPKIKEE
                     YLTNKKLKDDPRITPVGHFLRKTSLDEWPQFVNVLKGEMSFIGPRPYLPHEKEDMGQY
                     YDSIIKLKPGVTGMWQANGRSDVEFSYRCKLDDYYYHNWSIWLDFTIMYKTVKSVVYG
                     KGSL"
     gene            2827..3303
                     /locus_tag="PLK68_03260"
     CDS             2827..3303
                     /locus_tag="PLK68_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM89463.1"
                     /translation="MKFFYTGLNKFMWNHPFIKSCTHFTSRFCPYMVAIFYMLFLLKI
                     YLDRPHNLLGLAAEPIAVFAITAILRIVIDRKRPSEKYDLTPIDGSKKTGHSFPSIHV
                     AMSISIALAVLHFGPNMGLLLSTLAIAISLCRLLSGVHYLTDILASIAIAFIVNLI"
     gene            complement(3571..>4336)
                     /locus_tag="PLK68_03265"
     CDS             complement(3571..>4336)
                     /locus_tag="PLK68_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM89464.1"
                     /translation="GWMSYADEGKMAGTTNQKKRMEAVKIKLSDFDGNSGLTYRTHVS
                     DVGWQNWVSSNERAGTEGQNKPIEAIEISLSRTMSDFFDIYYRMHVSNMGWLGWAKNG
                     ETAGTIGGRIQSEAIEIKLVPRNSTFDRGGVAFVDATITIRDRIVDAAHSRLGCPYVW
                     GGNGPNTFDCSGLVKWCYAQVGISIPRTTGELKSYGTQISVSQALPGDILWKSGHVGI
                     YIGNGQYIHAPQTGDVVKISSVSSGNFTFARRSNEL"
     assembly_gap    4337..4346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4347..4939)
                     /locus_tag="PLK68_03270"
     CDS             complement(<4347..4939)
                     /locus_tag="PLK68_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89465.1"
                     /translation="MKKFLKLFVVVMLTCTTLVFTLGIFTSPLSLSAMELPAINYQAH
                     VEDKGWLDTVHDNEIAGTVNQSKRLEALILNLKENDKSMVRYRAHIADVGWQAWVTSG
                     AQAGTTDKAIAIQAFQAELTNEYKDMYDIYYRVHVPYRGWLGWAKNGEIAGSIGLALR
                     TEAIQIKLVIKGSQISTEGLPSLTKPTLTYSSHVQDMG"
     gene            complement(5599..5730)
                     /locus_tag="PLK68_03275"
     CDS             complement(5599..5730)
                     /locus_tag="PLK68_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89466.1"
                     /translation="MGLCFLMMMKKIEIGSNDFGFTDDIQIKVNDREYLVSAVITKV"
BASE COUNT         2031 a         1104 c          622 g         2229 t
ORIGIN      
        1 cattactttc taaatattct ttttgcacct ctaaccggtt gttaaatgat atatcatcag
       61 catccattct acatatataa cgaccctttg ttaaagttaa tcctttattt aatgtttttg
      121 ttaaacccat atttttttca ttgataaaaa aacgtatacg attatctatt tttgcatact
      181 tatttattaa tttaatatgt tcactatttt cgggattatc gagaattatt atgaactcaa
      241 aatctttgta tgattgattt aaaatagatt ctatagattg ctttaaaaaa gattgctctt
      301 ctttataagt tgccattata actgatgttt ctatcatttt gcacctcact aattctttgt
      361 aataatatca taattattct tatgtaacaa tgaaaatcaa aatccataat aaataatcca
      421 ttaatctggt ttataaaaat ccacaacatt gtcctttgtc ttctctattt ttaatttata
      481 agcattataa tcctttgcca tgtggccaat atcccatgca gtataaccta atttagataa
      541 ctcataaaca agcactttag atgtcggtcc taaaataaag cataaagtct tatccttgct
      601 ataggtttta gatttggaaa ataattcatc ataactatca aaagcatttt tagaaggtcc
      661 atacacaatt tctttacttt tggctaattc aaaaatatca aattgaatat tatctaatac
      721 atttttaccc acgaaaagaa caatttcttt atctttaaac aagcttttta attttttata
      781 ataatcatcc aaatcaatat tgtaaatcat atacatttga ttaaaccctg ctgctatata
      841 ttttctatca ttattacaat aatttaataa aaactctcta tattgttttg cattgaccat
      901 ataaaacctt ctattaaatt catttaaatt tttagtagaa tgaaagtaat tatagttgat
      961 ccccacataa cacttgtcac tgttactctt taatatctca aacattttct cagataaaat
     1021 tctatcaaat ttttgaaaag caattgattg tccctgcatt aacaatattt ctccatcacc
     1081 aaatctacaa aaacttttag gtttatgtaa caatatatct attgtttctt cagcgcttat
     1141 aacatctaat ttagcagcac tttctaagtt atccattatt tcatatttgt aaacatcttc
     1201 cactcttatc aacgcctcat ataatgtttc cttttctcta tcttctattg aatatttaaa
     1261 aaggtatatt atgtttttta ttacattttt aattatttta accattaatt aatcctcact
     1321 ctctataacg aaccttttcc ataaacaaca cttttaacag ttttatacat aattgtaaaa
     1381 tctaaccata ttgaccaatt atgataataa tagtcatcta atttacaccg ataactaaac
     1441 tccacatcac ttcgaccatt agcttgccac atccctgtaa caccaggttt taatttaata
     1501 attgaatcat aatattgacc catatcctct ttttcatgtg gtaagtatgg tcttggccct
     1561 ataaaactca tttcaccctt taatacattt acaaattgtg gccactcgtc taacgatgtt
     1621 ttccttaaaa aatgtccaac aggagtaatc cgaggatcat cttttaattt tttgttagtt
     1681 aaatactctt cttttatttt aggatcctct tccatcattc tttcaagttc agcttcggca
     1741 ttaggtatca ttgatctaaa tttatatatt ttaatcaact ttccattttt cccaatccgc
     1801 tcttgtgaaa acatgatatt gtcatggtca ccgctcttaa catatttata tttcacataa
     1861 gccattaatg gcagtaaagt catcacccct gctataccag ctaatatatc aataagtctc
     1921 ttaataaact tatctaataa tccaatttca tcatttgacg ttgcgattag tagctgtcca
     1981 tcaaaatctt gaacttctga agaaaacgtc attgttccat tcacatctgg taaatactta
     2041 actgaatcaa ctttaccata tatatcgctc attaccttat caattacttg ctgatctgct
     2101 tctggtattg caataatgat ttgttcaata cctaggtttt ctattgcttc atcaattttg
     2161 tcatattcat atataggact atgcttaata atattttctt gttcaaatct aaataattca
     2221 tttgttttat ttacatcaac atatcccatt atattagtta aagcaaatct attattattt
     2281 gatatttttc ctaatcttgc tgcctcgtat ccggtaccaa taactaatgt tttcctcgca
     2341 aattgttctc taaacgcaat tctaagaaat ctagatgcta ataaactaat caaaaacatt
     2401 gatatcgcca taagcgttaa atgcattttt ctttcgtaac ctgtagattg gggtactaaa
     2461 actaataaag ctatgtaaaa acaaaaactt gatttgacaa gctgttgaat ttcattccat
     2521 attaaacaag tatttaatga ataatgtccg taaataaata aaaataaaaa atatagcgtt
     2581 cctgatccca ctattaaatc aatatccata tgaaaatata agcatataaa ataatacatc
     2641 acaacctcaa tcataaataa catgattggc attatttttc tattcataat tccacctcga
     2701 cattcataga acaattatac cttaatacaa ctattatttc tatcttttat cagtttaaac
     2761 aactaataag attgtttaaa ctgataaata ttgtataata attatatact ttataggagg
     2821 ttgtccatga aattctttta tacgggatta aataaattta tgtggaatca cccatttatc
     2881 aaatcttgta ctcattttac ttcaagattt tgtccataca tggttgctat tttttatatg
     2941 ctctttttat taaaaatata tttagatcga ccacacaacc tattaggttt agcggcagaa
     3001 ccaatagcag tatttgcaat taccgctatt ttaagaattg taattgatag aaaacgcccc
     3061 tctgaaaaat atgatttgac accaattgac ggcagtaaaa aaacgggtca ttcatttcca
     3121 agtattcatg ttgctatgtc aatatcaatt gctttagctg ttttacactt tggtcctaat
     3181 atgggattgc tattaagtac ccttgcaata gctatctcat tatgtagact tttatcaggt
     3241 gttcactact taactgatat tcttgcaagt attgctattg cttttatagt taatttaatt
     3301 taataacttt ttaatcacaa gataaaaaat attcttgtga tttttattaa ataatactgg
     3361 gtgggattaa aggatgcata tagtaatagt aaaatatcca acatgtaatt aagaattaga
     3421 aaacaatata tctattttcc aaggttcaaa actaaaatta aaaatagtga tgagtataaa
     3481 aggtagataa ttttgatatt aaacatttaa ctaaataatt tagaaaaaga ctcgacttca
     3541 cttcaaaagt acgagtcttt aatatattct ttataactca ttagatcttc tagcaaaagt
     3601 aaagttacca ctcgatactg aagaaatctt aactacatca ccagtttgag gtgcatggat
     3661 atattgacca ttaccaatgt aaataccaac atgacctgac ttccataaaa tatctcctgg
     3721 taaagcttgt gatacactta tttgagtacc ataactcttt aattcacctg ttgttcgagg
     3781 aatactaatt cctacttgag catagcacca cttaacaagc ccagaacaat caaatgtgtt
     3841 gggaccattt cctccccaaa catatgggca tcctaatctc gaatgcgctg catcaacgat
     3901 acggtcacgt atcgtaattg tagcatctac aaaagccaca ccacctctat caaatgtgga
     3961 gtttctagga acaagtttaa tttcaattgc ttctgattga atccttcccc ctatcgtacc
     4021 tgctgtttct ccattctttg cccaacctaa ccaacccata ttggaaacat gcattctata
     4081 atagatatca aaaaaatcag acattgttct tgataaacta atttcaattg cctcaatagg
     4141 tttattttga ccttcagttc ccgctctttc attgctagag acccaattct gccaacccac
     4201 atcagaaaca tgagttcgat atgttaatcc actattacca tcaaaatcac ttaatttaat
     4261 tttaacagct tccattctct ttttttgatt agtagttcct gccatcttac cttcatctgc
     4321 ataactcatc catcccnnnn nnnnnnccca tatcttgcac atgagagcta tatgttaatg
     4381 ttggttttgt tagtgatggt aatccctctg ttgaaatttg acttccttta ataactaatt
     4441 taatttgaat cgcttctgtt cgaagtgcaa gtcctatcga tcctgcaatc tctccattct
     4501 ttgcccagcc tagccaaccc ctatatggca cgtgtacccg ataataaata tcatacatat
     4561 ctttgtactc atttgtaagc tccgcttgaa acgcttgtat agctattgct ttatctgtag
     4621 tgcctgcttg agctcctgat gtaacccatg cttgccaccc aacatcagct atatgagcac
     4681 gatatcttac catactttta tcattttctt taagatttaa aattaatgcc tctagtcgct
     4741 tactttgatt tacagttcct gcaatttcat tatcatggac tgtatctaac caacctttat
     4801 cttcaacatg agcttgataa tttattgctg gtaactccat tgcacttaat gaaaggggtg
     4861 aagtaaaaat gcctaatgta aaaactagcg tcgtgcatgt aagcattact accacaaata
     4921 actttaaaaa ttttttcata aaacttttct ctgtatatta gaatctaatc tgttatcatc
     4981 aaatcattaa caaaaaattc aatatacttt tcctttcctg atttttattt agaattgtta
     5041 aagtgaatct atattttaaa ccaatccgta cataatttag cttttttcac aacctacttt
     5101 tctattatca aaatatagtt ttattaaata tatagaaatt ttaataaata atttttaaat
     5161 caataaaata ttatatcaaa aaatagaaga gccaaaaact taactctcct acttcttcca
     5221 tataactaat attaatattt tgaatactaa tataaccaat taaaatattt aacatacaaa
     5281 aaacagttaa atactccaat ccaattttaa ataaagacaa tattatcttt ataaaactat
     5341 acttgcactc atgtcacaga tagtttttcg agcattctct cctataatgc ttacgaaata
     5401 atttatggtt atacaataag aaagctattg actattaaaa ttaataagaa aaatgttata
     5461 attaatattt ctggtatata cttttcttca cttaacaaca ttctaaaaac tattaattaa
     5521 ttttataaaa cttaacgact ttagtacagt atatttagag ttactcttct aaacagaatt
     5581 ttttacacat tatatttact aaactttagt aattacagca cttactaaat attctctatc
     5641 attgactttt atttgaatat catcagtaaa gccaaaatca tttgatccaa tttcaatttt
     5701 tttcatcatc attaagaaac acagtcccat aatatgaatt atcaccacat agactaatat
     5761 tatatattcc agcctctaaa tccactccaa ccttaaatac accattattt taaagattta
     5821 atgttgatcg ctcatcaaca aataacactg tttcattaga ttgatatgtc aagcccatat
     5881 cttcgtaatt ttcataaaaa ataaatactt gacctacttt ttatttctag caaatgcatc
     5941 aacacttgcc tcaagatttt ctccatattc tgttgcatac ccagcagctt ctaaaa
//
LOCUS       DAOMTA010000077         5987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_247_length_5987_cov_2.248651, whole genome shotgun sequence.
ACCESSION   DAOMTA010000077 DAOMTA010000000
VERSION     DAOMTA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_247_length_5987_cov_2.248651"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /gene="trkA"
                     /locus_tag="PLK68_03280"
     CDS             complement(<1..907)
                     /gene="trkA"
                     /locus_tag="PLK68_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRM89467.1"
                     /translation="MNVIILGAGKVGKTLIKHMSNEDHDIIVIDQDAQKIEDVVNLYD
                     VIGVVGNGGSYDILMEAGATTANLIICVTASDELNILAGLMAKQMGTRHTIARVRNPD
                     YSKQRDFMRNQLGFSMIINPELEAANEIRRSLSFPSAVKVDTFARGKVELAEFYVEED
                     SYLRDIPLSQLHSITKTNILVCAVSHNEEVIIPDGNYIIKPGDHLFITGSHRDLSRFC
                     LDIGIVTNRIKNVLIVGGGKIAYYLAKQLGVQGIKVKIIEKDIEHCRVLAKKLPFVTV
                     INADGSDEEVLLEEGLETTDAFLALT"
     gene            complement(1250..1915)
                     /locus_tag="PLK68_03285"
     CDS             complement(1250..1915)
                     /locus_tag="PLK68_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM89468.1"
                     /translation="MNSKQYAVLGLGIFGSTVATTLAEYGCEVIAVDQDESCVERVAD
                     EVTKAVVANVTDQEELRAIGIEDIDVAIVAIGTHLEEAVLATMNLKELGVPYVIAKAK
                     NKQFMKILEKVGANKVIRAEKDMGLKVAKSLLRKSIVDLVELDEDYSVVEIKAPLDWV
                     GKNFIDLNIRRVYNMNIIGIKHGDEDHLSLDVAPEYVIQNGDHFLVIGKTKELERFDY
                     MTK"
     gene            complement(1931..3316)
                     /locus_tag="PLK68_03290"
     CDS             complement(1931..3316)
                     /locus_tag="PLK68_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRM89469.1"
                     /translation="MKVSTLKMHHEHHPMRPTRQIAISFLAVILIGSVLLMLPICNNT
                     TPTAYLNNLFIATSATCVTGLVPVVTSEQFNILGQIVIIILIQIGGLGFLTFLNLLLI
                     MVKKKISLTNKLVLQEALNQPSLNDLPKFVKNVIKYTFIVEGLGAIILAFVFIPDFGI
                     VKGIYYSIFHSISAFCNAGFDVLGANSLIGYQNNLIINLVIPGLIIMGGLGFIVWFDV
                     AHCIKKEYGKRSKFSKKHLFKSFSLHTKLVIIATVVLLLAGAVLFYLCEFNNIKTIGG
                     LPLFDQIQISFFQSATLRTAGFASVDMASLHPSTKLMMCVLMFIGGSPAGTAGGIKTV
                     TFAIGVLEVYNIYHGRKEVTAFSRRIPKRLIISMALAIVFLSIFALSITEQAPFIDIC
                     FEVVSAGATVGLSASLTPYLSVFGKIVIIMLMYIGRIGPITMMISFARKSYMQASKKE
                     VRYPDGNILLG"
     gene            complement(3328..3888)
                     /locus_tag="PLK68_03295"
     CDS             complement(3328..3888)
                     /locus_tag="PLK68_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRM89470.1"
                     /translation="MSIIEIALIGVGLAMDAFAVSICKGLAMRRMNYKKAIIIAAFFG
                     VFQALMPALGYVLGTTFANKIAAIDHWIAFILLALIGANMIKEALSSDDDECQDDSLR
                     LGDLIMLSIATSIDALAVGITFAFFNVSLLLSVSMIGIITFIICVIGVKVGNVFGEKY
                     KSKAELAGGLILIVMGAKILIDHLFF"
     gene            complement(3896..5467)
                     /locus_tag="PLK68_03300"
     CDS             complement(3896..5467)
                     /locus_tag="PLK68_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89471.1"
                     /translation="MLLINNVKVPLELADYRKIISQQLNISKNKIFDVKLVKQAVDAR
                     RKNKVHFVCSFNFTVENEDLMIKKYPKLNLSKVVAYDYPVLKSTDEHIVVVGSGPAGL
                     FCAYNLARAHQKVTLIERGNAVEQRKEDIDNFFKTGKLSPDSNVQFGEGGAGTFSDGK
                     LTTGVKDKRKKFILETFVKHGAESDILYVNKPHVGTDYLIKVVKSMRETIIANGGEVL
                     FETKLVDVNLADDQLVNIVVEKNNIKTTMELDKLVLAIGHSARDTYEMLYQKGIKMEQ
                     KSFAVGLRIEHLQSFINEHQYGKYANHPSLKAADYKLAVKTSNGRGVYTFCMCPGGKV
                     INSSSEAGGIVVNGMSNQARDEANANSAVLVTVGPEDFASSHPLAGITYQRELEQKAF
                     ELGGKDYSVPVMRVEDYLNDTLDLKMEEVSCSVQPNVRYAKLSQIFSNEVNLALKEGL
                     QLMNHKFTGFTEKAMLSGVESRSSAPVRLYRDENFQSNIKGIMPIGEGAGYAGGIMSS
                     AIDGLKCSEFILKGE"
     gene            complement(5458..>5987)
                     /locus_tag="PLK68_03305"
     CDS             complement(5458..>5987)
                     /locus_tag="PLK68_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM89472.1"
                     /translation="CKASLLVDGKLIESQNGEVLFSDNGLSGICIMQLSRLLSRYCGN
                     KEISLDLMEEYTSQELERFLKERHDRFGHYYLEGIFNDKLAKILKDIDNLKDIRFKII
                     DTYDYSKAQVMSGGVSLDEIDENLEVIKYPGIYLAGELLDVDGDCGGYNLHFAFASGH
                     HIAKTIMNKQVKKCY"
BASE COUNT         2028 a         1237 c          674 g         2048 t
ORIGIN      
        1 ctgtcaatgc taaaaaagca tctgttgtct ctaaaccttc ttctaataat acctcttcat
       61 cacttccatc tgcattaata acagtaacaa acggtaattt tttagcaagc acacgacaat
      121 gttcaatatc cttttcaatg atttttactt ttattccttg aacccctaac tgtttagcta
      181 aataataagc aatctttcct ccaccaacaa ttaaaacatt ttttatccta tttgtaacaa
      241 tgccaatatc taaacaaaat cttgataagt ctcgatgtga tcctgtaata aataaatgat
      301 cacctggttt aataatataa ttaccatctg gtataattac ttcttcattg tgacttacag
      361 cacatactaa aatattagtc ttagtaatac tatgtaattg acttagcgga atatcacgta
      421 aataactatc ttcttctaca taaaattctg ccaattcaac ttttccacga gcaaaggtat
      481 caacctttac agccgaagga aatgataatg atcttctaat ctcatttgct gcttctaatt
      541 caggattaat aatcattgaa aaacctaact gattacgcat aaaatcacgt tgtttagaat
      601 aatcgggatt tcttactcga gcaattgtat ggcgagtccc catttgtttt gccattaatc
      661 cagctaagat atttaattca tccgatgcag taacacaaat aatcaaattt gctgtagttg
      721 cccctgcctc catcaagata tcataagaac caccattacc tacaactcca atgacatcat
      781 ataagtttac tacatcttct attttttgtg catcctggtc aatcacaata atatcatgat
      841 cctcgttgga catatgctta attaaagttt ttccaacctt accagcaccc aaaattataa
      901 cattcataaa gaatagcctc ccttgataat aaccaaattg tagtccattt tcctatattt
      961 ctcaatagga attcggcaaa tacataagaa gtttaccaaa ttgaaacatt tttacaagga
     1021 cttaataagt tcttataaga ttaacaattt ttatatcatt taattttaaa atagatttct
     1081 cattttcatt aactatgatt cattagtata aatttaaata acttagccta tattagcctt
     1141 cgtaaaccat ttataacaaa ataaaaaagg actaaataaa ctaaatccta tagatgacac
     1201 tcctctttat tttgtgtctt ttctatagga tttagtttaa aaagtgtcct tatttagtca
     1261 tataatcgaa tctttccagt tctttagtct taccaattac caagaaatga tccccatttt
     1321 ggatcacata ttctggagca acatctagag aaagatgatc ttcatcacca tgtttgattc
     1381 caataatatt catattataa acacgacgaa tatttaaatc aataaaattc tttcctaccc
     1441 aatcaagtgg tgctttaatt tcaactactg aataatcttc atcaagttca actaagtcaa
     1501 caatactttt tctcaataaa ctcttagcta ctttaagtcc catatctttt tcagcacgaa
     1561 taactttatt agcccctact ttttcaagaa ttttcataaa ttgtttattt ttagccttgg
     1621 caataacata tggaacaccc aattctttta aattcatagt tgctaaaaca gcttcttcca
     1681 aatgtgttcc gattgcaaca atagcaacat caatatcctc aataccgata gctcgcagct
     1741 cttcttgatc agtaacattc gctacaactg ctttcgttac ttcatcagct acccgctcaa
     1801 cacatgattc atcttgatca acagcaataa cctcacagcc atattctgcc aatgttgttg
     1861 cgactgttga accgaaaatt cctaatccta atactgcata ttgtttacta ttcatcttta
     1921 taccctcttt ttatcctaat aagatattcc catcaggata ccttacctct tttttacttg
     1981 cttgcatata tgattttctt gcaaaagaaa tcatcattgt aattggtccg attcgaccaa
     2041 tatacatcaa cataattatt acaattttac cgaaaacact taaatacggt gttagtgaag
     2101 cacttaatcc taccgttgct cctgcagaaa caacctcaaa acaaatatca ataaatggtg
     2161 cctgttcggt aatactcaaa gcaaatatac ttaaaaatac aattgctaat gccatagaaa
     2221 taattaaacg tttaggaatt cttcttgaaa aagctgttac ttctttgcgt ccatgataaa
     2281 tattgtatac ttctaataca cctattgcaa acgttactgt tttgattcct ccagcagtac
     2341 ctgcaggtga accaccaata aacatcaata cacacatcat caacttcgtt gaaggatgta
     2401 aacttgccat gtcaactgaa gcaaacccag ccgttctaag agttgctgat tgaaaaaaag
     2461 aaatctgtat ttgatcaaat agaggtagtc ctccaattgt tttgatatta ttaaattcac
     2521 acaaataaaa taaaacagct cctgctaata acaatactac tgttgcaata ataaccagtt
     2581 ttgtatgtaa tgaaaaactt ttaaataaat gttttttact aaatttcgac cgttttccat
     2641 actctttttt tatacaatga gcaacatcaa accaaacaat aaatccaaga ccacccatta
     2701 taatcaaacc cggaataacc aaattgataa ttagattatt ttgatatcca atcaatgagt
     2761 tagctcctag cacatcaaat ccagcattac aaaatgctga aatcgagtga aagattgaat
     2821 aataaattcc tttcacgatt ccaaaatcag gaataaatac aaatgctaga atgattgcac
     2881 ctaacccttc aacaataaat gtatatttaa ttacattttt tacaaactta ggcagatcat
     2941 ttaacgatgg ctgatttaaa gcttcttgta aaaccaactt atttgtaaga gatattttct
     3001 ttttgaccat tattaataat aaatttaaaa aagtcaaaaa acctaaccca ccaatttgaa
     3061 ttaaaataat aataacgatt tgtcctaata tattgaactg ttcacttgtt actacaggta
     3121 ctaatcctgt tacacaagtt gctgaagtag caataaataa attatttaaa tatgctgtcg
     3181 gtgttgtatt attacaaatt ggcagcatta acaaaactga accaattaag ataactgcta
     3241 aaaagctaat ggcaatctgt cttgttggtc gcataggatg gtgctcatga tgcatcttta
     3301 aagtcgagac cttcattttt tatcctccta aaagaacaag tggtcaatta atatttttgc
     3361 gcccatcaca attaatatta atccaccggc taattctgct ttactcttat atttttcacc
     3421 aaatacatta ccaactttga ccccaattac acaaataata aaagtaataa ttccaatcat
     3481 gcttactgaa agcaataatg acacattaaa gaacgcaaaa gtaatgccaa cagctagggc
     3541 atctatgctg gtagcaattg acaacatgat caaatcccct aatctcaaac tatcatcttg
     3601 acattcatca tcatcacttg ataatgcttc tttaatcata ttcgccccga ttaaagctaa
     3661 taagataaaa gcaatccagt gatcaatagc tgcaatctta ttggcaaatg ttgttcctaa
     3721 aacataacct aatgccggca ttaaagcttg aaacaccccg aaaaaagcag caatgataat
     3781 agctttttta tagttcattc ttcgcattgc taaaccttta cagattgaaa ctgcaaaagc
     3841 atccattgct aagcccactc caatgagggc tatttctatt attgacattc cttatctatt
     3901 ccccttttaa aataaattca ctacatttta aaccatctat tgcactagac ataattccac
     3961 cagcataccc agcgccttca ccaatcggca tgattccctt aatattactt tgaaaatttt
     4021 catcacgata taagcgtact ggtgctgaac ttctagattc aactcctgaa agcattgctt
     4081 tttctgtaaa ccctgtaaat ttatgattca ttaattgtaa tccttctttt aaagcaagat
     4141 tgacctcatt actaaatatt tgacttaatt tagcatatct cacatttggt tgaacactgc
     4201 agcttacttc ttccattttt aaatccagag tatcatttaa ataatcttca accctcatga
     4261 ctggcacact ataatcttta ccacctaatt caaaagcttt ttgttctaat tctcgttgat
     4321 aggtaatacc agctaatgga tgcgaactag caaaatcttc aggtcccaca gtaaccagaa
     4381 ctgcactatt agcatttgct tcatcacgag cttgattact cattccatta acaacgattc
     4441 caccagcctc actacttgag tttataactt tacctcccgg acacatacaa aaagtatata
     4501 ctccacgacc attactagtt ttgaccgcta atttataatc agcagctttt aaacttggat
     4561 gattcgcata tttaccatat tgatgctcat ttatgaatga ctgcaagtgt tcaattcgaa
     4621 gtcctaccgc aaatgatttt tgctccattt ttattccttt ttgatatagc atttcataag
     4681 tgtctcgagc actatgacca attgctagaa ctaatttatc taattccatc gtagttttaa
     4741 tattattttt ttcaacaaca atgtttacta gctgatcatc tgctaaatta acatcaacta
     4801 attttgtctc aaataacact tcaccaccat tagcaataat cgtctcacgc atacttttaa
     4861 caaccttaat taaataatca gtccctacat gtggcttatt tacatataaa atatcacttt
     4921 cagcaccatg cttaacaaag gtttcaagaa taaatttttt tcgtttatcc ttaaccccag
     4981 ttgttaattt gccatcacta aaagttcctg ctcccccttc cccaaattga acattactat
     5041 caggacttaa tttccctgtt ttaaaaaagt tatcgatatc ttctttcctc tgttcaaccg
     5101 cattgccacg ttcaatcaat gttacctttt gatgagcccg cgccaaatta taagcgcaaa
     5161 aaagtccagc gggaccactg cctactacta caatatgctc gtcagttgat tttaaaaccg
     5221 gatagtcata agcaactact tttgataagt ttaatttagg atattttttt atcattaaat
     5281 cttcattctc gacagtaaaa ttaaaactac aaacaaagtg aactttgttt tttcttcttg
     5341 catcaacagc ctgtttaact agtttaacgt caaatatttt atttttagaa atatttaatt
     5401 gttgtgagat tatttttcga taatctgcca gttccaaagg aacttttaca ttatttatta
     5461 ataacatttt tttacctgct tattcattat tgttttagcg atatgatggc ctgaagcaaa
     5521 agcaaaatgt aaattatatc cgccacaatc accatcaaca tctaaaagct ccccagccaa
     5581 atatattcca gggtatttaa tcacttccaa attttcatct atttcatcca aactaacccc
     5641 accactcata acttgagctt tactgtaatc atatgtatcg ataattttaa agcggatatc
     5701 ttttaaatta tcaatatctt ttaatatttt agctaattta tcattgaaga ttccttctaa
     5761 ataataatga ccaaatctat catgccgctc ttttaaaaat cgttctaatt cttggctagt
     5821 atattcctcc attagatcta aactaatctc tttatttccg caataacgtg ataataatcg
     5881 tgataactgc ataatacata ttcctgataa cccattatca ctaaataaca cttcaccatt
     5941 ttgactttca attaattttc catctactaa taaactcgct ttacatt
//
LOCUS       DAOMTA010000078         5958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_250_length_5958_cov_2.056412, whole genome shotgun sequence.
ACCESSION   DAOMTA010000078 DAOMTA010000000
VERSION     DAOMTA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_5958_cov_2.056412"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..684)
                     /gene="hisF"
                     /locus_tag="PLK68_03310"
                     /pseudo
     CDS             complement(1..684)
                     /gene="hisF"
                     /locus_tag="PLK68_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
     gene            complement(666..1391)
                     /gene="hisA"
                     /locus_tag="PLK68_03315"
     CDS             complement(666..1391)
                     /gene="hisA"
                     /locus_tag="PLK68_03315"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRM89473.1"
                     /translation="MLVIPAIDLKDGQAVRLFKGDYNQKTVYSNEPEKLAENFEKMGA
                     KLLHVVDLDGAKDGECINLETIKKIKQNTSMQVELGGGIRNIETVALYLDEVGIDRVI
                     LGTAAINDPEFLKSAINTYGPEKIVVGVDVKDGFVSTSGWLKISNVPYLEFIKELEKL
                     GVKYIVATDISKDGTLQGPNFDMYEQIARTSTINFVVSGGIKDAQNIKDVASKNYYAC
                     IVGKAYYEGKVDLKEVITCLQNG"
     gene            complement(1385..1993)
                     /gene="hisH"
                     /locus_tag="PLK68_03320"
     CDS             complement(1385..1993)
                     /gene="hisH"
                     /locus_tag="PLK68_03320"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRM89474.1"
                     /translation="MVVIIDYNIGNLSSVISALKRVGIEAVITRDKDIIRQAKAIVLP
                     GVGAFPVAMNNLKKFDLIDVLNERKDAGIPILGICLGMQILFEKGYEVEETKGLGFLD
                     GEVVFMDIDEKVPHMGWNQLHFNQNHPILKNIHENDDVYFVHSFMATCPNEQLIAYSD
                     YGKTNITAIAAKDNVIGCQFHPEKSGAVGQKILLAFKEMIEC"
     gene            complement(2013..2600)
                     /gene="hisB"
                     /locus_tag="PLK68_03325"
     CDS             complement(2013..2600)
                     /gene="hisB"
                     /locus_tag="PLK68_03325"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRM89475.1"
                     /translation="MRIGKIERETKETKILVQLDLDGEGKSEISTGIGFFDHMLTLFA
                     FHGGFDLIVKCEGDLEVDTHHTVEDLGIALGTCLKEALGNKLGIKRYGAFTIPMDETL
                     VTTNLDISGRSFLVYNVNLTCERIGTFETEMTEEFFRALAFNSLITLHINEQYGTNNH
                     HIVEAIFKSLGRALKEAVSIDEANKDKVVSSKGVL"
     gene            complement(2603..>2876)
                     /locus_tag="PLK68_03330"
     CDS             complement(2603..>2876)
                     /locus_tag="PLK68_03330"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRM89476.1"
                     /translation="KIKNAGSIFLGEYSPEPLGDYMSGTNHVLPTGGTAKFYSALGVY
                     DFVKYSSYSYYPKNVLGEFKEDVIKFAKSEGLDAHANSVAVRFEEE"
     assembly_gap    2877..2886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2887..3952)
                     /gene="hisD"
                     /locus_tag="PLK68_03335"
     CDS             complement(<2887..3952)
                     /gene="hisD"
                     /locus_tag="PLK68_03335"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRM89477.1"
                     /translation="MLKIIDYKGNLEAVATKLDSRKESVNKEVNEAVLKIIEDINERR
                     NKALYEYCLKFDGYQINDEKDLIVSEIEKEEALKQIDADYLRILERTKEQITEFHKNQ
                     IDKSWSLFKDNGVIMGQMVRPIERVALYVPGGTASYPSTVLMNAVPAKLAGVKDLVII
                     TPVKEDGKVNPIIIAAAKVSGVDTIYKFGGAQGVAAIAHGTETIKKADKIVGPGNIFV
                     ATAKKLSYGLVDIDMVAGPSEVLVIADENANPKYIAADLMSQAEHDKLASALLVTTSR
                     DLVAKVNGELVRQMAYLSRRDIIEESLVNYGGAIIVDNLNEAFDVSNYLAPEHLEVLV
                     DNPVNMLPKIKFEIIVSDEVW"
     gene            complement(3952..4581)
                     /gene="hisG"
                     /locus_tag="PLK68_03340"
     CDS             complement(3952..4581)
                     /gene="hisG"
                     /locus_tag="PLK68_03340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRM89478.1"
                     /translation="MIKIAITKGRIEKQVCKLLQEAGFDMEPIFNKDRELLIATKDGI
                     EMIFGKANDVVTFLEHGIVDIGFVGKDTLDDVDFEDYYELLDLNIGKCYFAVAAYPEY
                     RNKKFERRKKIASKYTKVAKKYFATKQEDVEIIKLEGSVELGPIVGISDAIVDIVETG
                     STLKANGLEVIEKISDISTRLVANKVSFKFKKDEIMNLVNKLEAVTEGK"
     gene            complement(4574..5554)
                     /locus_tag="PLK68_03345"
     CDS             complement(4574..5554)
                     /locus_tag="PLK68_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRM89479.1"
                     /translation="MEEFKYLIPEESIDAIITNVSKLREIENSLRAIFAKNNYNEVLM
                     PSFEYVDLYTQLDCGFEQEKMFQYINHEGKNVAMRCDFTIPLARFYSTNNYGDEEARY
                     CYFGKVYRKETMHKGRSSEFFQGGVELINKPGIAGDKECLNMIQESLPHLGLKNILVE
                     IGSARFFNRLCTLVGDKAVELTEILKYRDISEMKKFVADNEFDGQLNELLLKLPTAFG
                     DITLLDTAIKGINDEILLKALERLKELYNSLDNKESIIFDLGMVPTMKYYTGLMIKGY
                     SDKSAQPIISGGRYDDLLPRFNKNVGAIGFCYHMNHILKAIDKQGEGNND"
BASE COUNT         1906 a         1260 c          666 g         2116 t
ORIGIN      
        1 acagttgctt cgccaaaatg aaataatgaa gccactaaag cggcatcaca attagtttgt
       61 ttaaagacat caacaatgtc ttgaatactg ccacaaccac cactggcaat tactggaata
      121 tcaacagcat tacataccgc attgatcatg tcgatatcat aacctgcttt agtcccatct
      181 gcatccattg aagtcaatag aatttcccca gcacccaatt cttcacattt agttacccat
      241 tctattagat caattccagt attttcacga ccacctttta cataaacgtc ataaccgtca
      301 tcatcacgtt ttttagcatc gattgcaacg actacacact gtaccccaaa ttcatcagca
      361 gccgctttta ttaaatcagg gtttgcaata gctgccgaat taacagatac tttatctgct
      421 ccactagcta agatcgttcg aaaatcatca agcgtccgga ttcccccgcc aacagctaga
      481 ggaatcgtta attcactagc cccgcgttca ataatatctt taataatatc acgttcttca
      541 taacttgcag taatatctaa aaacactact tcatcagcac attgctgatc ataacgttta
      601 gctaattcaa tgggatctcc aacatccttt tacaacttta ccatttttta tatccaaaca
      661 aggtattatc cgttttgtaa gcatgtaata acctccttga gatctacttt accctcataa
      721 taagccttac caacaataca tgcataatag ttcttactag ctacatcttt aatattttga
      781 gcatctttaa tgccccctga aacaacaaag ttaatagtag aagttctagc gatctgttca
      841 tacatatcaa agttaggacc ttgtaatgtt ccatctttac taatatctgt agcaacaatg
      901 tatttaaccc ctaacttctc taactctttg ataaactcca agtatggcac gttactgatt
      961 tttaaccagc ctgaagttga aacaaagcca tctttaacat caactcctac gacaattttt
     1021 tcaggaccat aggtattaat agcacttttt aaaaactctg gatcattaat tgcagcagtt
     1081 cctaaaatta cccgatctat tcctacttca tctaaatata aagcaactgt ttcaatattt
     1141 ctaatccccc caccaagttc tacttgcatt gaagtatttt gttttatttt cttaattgtt
     1201 tcaagattaa tacattcacc atctttagca ccatctaaat caacaacatg aagcagctta
     1261 gcacccattt tctcaaaatt ctcagctaat ttttctggtt cattactata tactgttttt
     1321 tgattataat cacctttaaa caatctaacg gcttgaccat cttttaaatc tattgctgga
     1381 ataactaaca ttcgatcatc tccttaaacg ctaataatat tttttgtcct acagctccac
     1441 ttttttcagg atggaactgg caaccaatca cattgtcttt agccgcaatt gccgttatat
     1501 ttgtcttacc ataatcacta taagcaatta attgttcatt tgggcatgtt gccataaaag
     1561 aatgaacaaa atagacatca tcattttcat gaatattttt taaaattgga tggttttggt
     1621 tgaaatgtaa ttgattccaa cccatatgag gaaccttttc atcaatatcc ataaagacta
     1681 cttctccatc taggaaacct aatcctttag tttcttcaac ttcataccct ttttcaaata
     1741 aaatttgcat tcccaaacaa attcccaaaa ttggtattcc agcatctttt cgttcattta
     1801 aaacatcaat taaatcaaac ttttttaaat tattcatagc taccggaaaa gcccctactc
     1861 caggtaatac aattgcttta gcttggcgaa taatatcttt gtcacgagta attacagctt
     1921 caattccgac cctttttaaa gcggaaataa cactacttaa attaccaata ttgtagtcaa
     1981 tgattactac catctaaatc cccccttgat ttttataaga ctccttttga tgaaacaact
     2041 ttatctttgt tagcttcatc aatactaacc gcttctttta aagctcttcc taaactttta
     2101 aatatagctt caacaatatg atgattatta gttccatact gttcattaat atgtaaagta
     2161 atcagtgaat taaatgccaa tgcccgaaaa aattcttccg tcatttctgt ttcaaatgtg
     2221 ccaattcttt cacaagttag attaacatta taaactaaga aagaacgccc actaatatct
     2281 aaattggttg ttactagcgt ttcatccatc ggaatagtaa aagctccata tcttttaatc
     2341 ccgagtttat tgccaagcgc ttcttttaaa caagttccta aagcaattcc aagatcttct
     2401 actgtatgat gagtatcaac ttctaagtca ccttcgcatt taacgattaa atcaaaacct
     2461 ccatgaaaag caaatagtgt taacatatga tcaaagaatc cgattccagt actaatttca
     2521 cttttacctt ctccatctaa atctaattgc acaagaattt tcgtttcttt tgtttcacgt
     2581 tcaatctttc caattctcat gactattcct cctcaaatct tactgctact gaatttgcat
     2641 gggcatctaa accttcactt ttagcaaatt taattacatc ttccttgaat tcgcctaaga
     2701 catttttagg ataatagcta taagatgaat atttaacaaa atcatatacc cctaatgccg
     2761 aataaaactt agcagttcct ccagttggta gtacatggtt agttccactc atataatcgc
     2821 ctaatggttc cggtgaatat tctcctaaga aaatagaacc ggcattttta atttttnnnn
     2881 nnnnnnccca aacttcatca cttactataa tttcaaattt aatttttggt aacatattta
     2941 caggattatc tactaatact tctaaatgtt ctggtgctaa atagttggaa acatcaaatg
     3001 cttcattcaa attatcaaca atgattgctc caccataatt aaccaaggat tcttcgataa
     3061 tatctcttct tgataaataa gccatttggc gaactaattc accatttact ttagccacta
     3121 aatcacgact tgtcgtaact aataaagcac tagccagttt atcgtgttct gcctgactca
     3181 ttaaatcagc agcaatatat ttaggattag cgttctcatc tgcaattacc aatacctcac
     3241 ttgggcctgc aaccatatca atatcaacaa gtccataact aagtttttta gccgtcgcaa
     3301 cgaaaatatt tccaggacct acgattttat cggctttctt tattgtttca gtaccatgag
     3361 caatggcagc caccccttga gcgccaccaa atttatagat tgtatcgacc ccacttactt
     3421 ttgctgcagc aataataatc ggattaactt ttccatcttc ttttaccggt gtaataatta
     3481 ctaaatcttt aactcccgct aactttgctg gaacagcatt cattaatact gtagaaggat
     3541 aactagcggt tcccccagga acatataatg ccacccgttc aattggacgc accatctgtc
     3601 ccatgattac cccattatct ttaaataaag accatgattt atctatttga tttttatgga
     3661 attcagtaat ttgttcttta gttctttcaa ggatacgtag ataatctgca tctatttgtt
     3721 ttaatgcttc ttctttttct atctcactaa cgattaaatc tttttcatca ttaatttgat
     3781 aaccatcaaa ctttaaacaa tactcatata atgctttatt tctacgttca ttaatatctt
     3841 caataatttt taatacagcc tcatttactt ccttattaac actttcctta cggctgtcta
     3901 atttagtcgc taccgcttct aaattgcctt tataatcaat tatttttaac attattttcc
     3961 ctccgtaact gcttctaatt tattaactaa attcataatt tcatcttttt taaacttaaa
     4021 actaactttg ttggccacta atctagttga aatatcacta attttttcaa tcacttctaa
     4081 gccatttgct tttaaagtcg accccgtttc tacaatatcc acaattgcgt cactgattcc
     4141 cacgattggt cccaattcta cagatccttc taatttaatg atttcaacat cttcttgttt
     4201 tgttgcaaaa tatttttttg ctactttagt atatttactt gcaatttttt tacgccgttc
     4261 aaatttcttg ttacgatact ctggataagc cgctacagca aagtaacatt tacctatatt
     4321 taaatccaat aattcataat aatcttcaaa atcaacatcg tctaatgtat ctttaccaac
     4381 aaaaccaata tcgacaattc cgtgttctaa gaaagttact acatcattgg ctttaccaaa
     4441 aatcatttca attccatctt ttgttgcaat taataattcc cgatccttat taaaaattgg
     4501 ttccatatca aatcccgctt cttgtaacaa cttacagacc tgcttttcaa ttctaccttt
     4561 agttattgct attttaatca ttattgcctt ccccctgctt atcaatcgct tttaaaatat
     4621 gattcatatg gtagcaaaat ccaattgcac caacattttt attgaatctt ggtaataagt
     4681 catcatatct tcccccagaa atgattggct gcgcactttt atcactatat cctttaatca
     4741 ttaagccagt gtagtatttc attgttggca ccattcctaa atcaaaaatg atactttctt
     4801 tattatccaa agagttatat aactctttta atctttccaa agcctttaac aatatttcat
     4861 catttattcc tttgattgca gtatctaata aagtgatatc accaaaagca gtcggcaatt
     4921 ttaataatag ttcatttaac tgcccatcaa attcattatc ggcaacaaac tttttcattt
     4981 cacttatatc ccgatacttc aatatttctg ttaattcaac tgccttatca ccaaccagtg
     5041 tgcataggcg attaaaaaaa cgtgctgaac caatttctac aagaatattt tttaatccaa
     5101 gatgtggtaa agactcttga atcatgttta aacattcttt atcaccagca attccaggct
     5161 tgttgatcaa ctcaactcca ccttgaaaaa actcactgga acgtccttta tgcatagttt
     5221 ccttacggta tactttacca aaataacaat aacgtgcttc ttcatcacca taattgtttg
     5281 ttgaatagaa acgtgctaat ggaattgtaa aatcacagcg cattgcaaca tttttacctt
     5341 catgattaat gtactgaaac attttttctt gttcaaaacc acaatctaat tgagtatata
     5401 agtcaacata ctcaaatgat ggcattaaca cttcattata gttattcttt gcaaagatag
     5461 ctcttaaact attttcaatc tctcgtaatt tcgatacatt agtaataatt gcgtcaatac
     5521 tttcttccgg tattaaatac ttaaattctt ccattttatt tcccccttta gttacaaaaa
     5581 aacgagtcat tacactaatg actcgctaaa gtaatattgt cattagatac acccaaataa
     5641 tatttaacac aaaatgggtt gtatcaataa actgataaaa ccctaggcaa tccaatgatg
     5701 gtgatgtaat tgtaaatttg ttgttgtttt cataattaac acctctcatt gtatacaata
     5761 tatcatatct aatgataaat gaaaagataa aattaattaa aattatagat ttatcataaa
     5821 gtttatttat ctaacttgtt aaaatcttaa atttttatct tcaacattac cattgccatt
     5881 tataatcaaa tatttaccaa aattcgtgag tattagcaaa tattcgttaa atattagcta
     5941 aaaaaggcga aatatttt
//
LOCUS       DAOMTA010000079         5950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_251_length_5950_cov_2.110263, whole genome shotgun sequence.
ACCESSION   DAOMTA010000079 DAOMTA010000000
VERSION     DAOMTA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_251_length_5950_cov_2.110263"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..799)
                     /locus_tag="PLK68_03350"
     CDS             complement(368..799)
                     /locus_tag="PLK68_03350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM89480.1"
                     /translation="MLIRKIEEVDYQDVYQLVKYELGYQNLEFNKFCWRLDQMLQDTN
                     YQIYVAIIDYRVVGIIGLMLGWGLEIEGKIMRIIALAVEHRYQGQKIGSSLITYSEQY
                     ALSKQVSAITVNSGLERRRAHEFYKKNDYYKKGYSFIKKCE"
     gene            complement(868..1449)
                     /gene="yqeK"
                     /locus_tag="PLK68_03355"
     CDS             complement(868..1449)
                     /gene="yqeK"
                     /locus_tag="PLK68_03355"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRM89481.1"
                     /translation="MMDLTIEEQIKDFYLLNNKERTYQHVLGVAMVAEKLALQYQEDI
                     RACIVAALLHDISAVITPQDMMKIANELQWQLDSAEKIYPFLLHQKISALIAYEYFQI
                     RNLSILKAIESHTTLRSNPSKLDMIIFIADKLAWDQPGEPPFKKNIEMALNDSLEAAC
                     YQYIKYQFDHDLLLFPHCWIIEAYRWLEKSHCK"
     gene            complement(1481..2050)
                     /locus_tag="PLK68_03360"
     CDS             complement(1481..2050)
                     /locus_tag="PLK68_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRM89482.1"
                     /translation="MENLYCYEQKLQSYLDEYHCILNQMINKMTSVELSCSISYNFMV
                     QMIPHHRAAILMSCNLLKYTKNEALQNIAFNIIKEQTRSIENMRRILCDCQNQTNSQE
                     QLYDYQNRLDIIVGTMFTKMAEPSCSNDIDVNFINEMIPHHEGAIAMAKTTLEYPICS
                     ELKTILQAIITSQSNGVRQLKTVLEDITC"
     gene            complement(2155..2231)
                     /locus_tag="PLK68_03365"
     tRNA            complement(2155..2231)
                     /locus_tag="PLK68_03365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2195..2197),aa:Arg,seq:tct)
     gene            complement(2289..2756)
                     /locus_tag="PLK68_03370"
     CDS             complement(2289..2756)
                     /locus_tag="PLK68_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM89483.1"
                     /translation="MKKVVVMSDSHGYHKMIDEVQRLEPDGDYYVHCGDSEAREEQLK
                     GWICVRGNNDWMAPFDDEVVFEVEGVRFLVTHGHRYGYYKREEAMVDDLLRHGCDVLL
                     SGHTHVPQCDEVTGFYLINPGSTTLPRRGSGKSYCTILVDQGKIEVKFKQIFC"
     gene            complement(2803..3720)
                     /locus_tag="PLK68_03375"
     CDS             complement(2803..3720)
                     /locus_tag="PLK68_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRM89484.1"
                     /translation="MKRKLRNGIIICACLMVCFFCIKQDNNRNEKKNTATMTANYQTV
                     VFRDDKNTLVPIEVDFGAEVEDDTKYRNMIEVMKSNDYEYLGLHPILDSNLQVNAMAI
                     NDKSLTFDLSDNLYVNSNQEALDIFEMFSYVFCNGDIEKVNLKIDGNDISTLPNSTVP
                     ASCITNQLGINNFEASTNYIYKTTPVVVYNTETINNKEYYVPVTKRIETNENDIDTKV
                     SIMLNEMDYDKPLSLVDQCSLQDGTLSIHLAANILNDNESIDNTLYNRIVKSASHLEN
                     VKKVSLFVDNQEIDPVQDVNGEVDNRIKM"
     gene            complement(3845..4627)
                     /gene="murI"
                     /locus_tag="PLK68_03380"
     CDS             complement(3845..4627)
                     /gene="murI"
                     /locus_tag="PLK68_03380"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRM89485.1"
                     /translation="MERAIGVFDSGVGGLTVLNSIRTLLPNEIIYIGDNYHCPYGEKT
                     REQLFSYASEIVEYFIKENVKLIVLACNTTSATVLNELQAVYKEVLIIGVIDATVEDF
                     ISRGVDNTLVIATAATINSHKYPDTIDCYQTGIEVFTLPTPKLVPLVEAGMDKKDIYD
                     VLHEYLDSYAGKIKSIILGCTHYPILENQIKDILPDIEYISSSDAVCKNVRDVLIKNN
                     LLNLNKNKFIKIYTTGRVDEFLKSSTGFFDYTDLVVEHIIIK"
     gene            complement(4630..>5670)
                     /locus_tag="PLK68_03385"
     CDS             complement(4630..>5670)
                     /locus_tag="PLK68_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM89486.1"
                     /translation="NRALIEMYLDDEEQDYLVENAIAVDQFIKYIEFPEFKLQYYEFY
                     NALNRTNKYSNYSDLINVGNQLASKLEVSFASNALSRCNTLIKNDLVNAYINQEGFSF
                     DNIDYYQLLRSLYDEGDYTYIADTNTYLSIMKEFDGLEGKKLYDNVKLLSNNFTKGSL
                     ASLFNHDLQPNAKRIYNPSDLALVVNNETYIGGYEPKNQVEIRGIPRVKYSMYLQEDA
                     NNSLMEMYRALNDEGYNDMVLTAAYISYDVASLGSSGILPGYNEYQLGTTINLQKREI
                     SIADFDQTDIYKWLINHCHEYGFILRYPSDKVDVTNHEYSSTTFRYVGKEIASKLHVQ
                     NLALEEYNANEE"
     assembly_gap    5671..5680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2010 a         1106 c          684 g         2140 t
ORIGIN      
        1 taacattttc aaactttttc ataaaaacac ttgaatttta tatagttacc tgcctctttt
       61 aaaaatcatc tttttttatt attatatcaa ggttattaat tgtttcaatt aataaaaata
      121 gagtaacgtc attagttgaa tccctctact cataacgtta actctaaaat ctattattac
      181 gcttttattt acttttaatg tcatcaatga tgattccatc ttcaattgta atgatgcggt
      241 ctgcctgata attcactaat aaaagcagtt tttttatttt taatatcaag caattctaaa
      301 acctcatcaa tattgtattc ctttgtcaaa ggtgaaattc aagaacaatt agtactttat
      361 tcacaactta ttcacatttc ttaataaagc tataaccttt tttataataa tcatttttct
      421 tataaaattc atgtgctcga cgacgttcaa gtccgctatt gactgtgatg gcacttacct
      481 gtttgctcag ggcgtattgt tcactatacg taattaaaga acttccaatt ttttgccctt
      541 gatatctatg ttctactgcc aacgcaataa ttctcataat tttcccttca atttctaacc
      601 cccaacctaa catcaatcca attatcccta ctactctgta atcgataatt gcaacataaa
      661 tttgatagtt tgtatcttga agcatttgat caagtcgcca acaaaattta ttaaattcca
      721 agttttggta tcctaattca tatttaacta attgatacac gtcctgataa tcaacttcct
      781 ctattttacg tattaacatt taataatctc ccttcttcct acagtttaaa cccccttaaa
      841 attttaagca aacaaaaagg ccacatgtta tttacaatgt gacttttcta accatcggta
      901 tgcctcaatg atccaacaat gaggaaataa taatagatcg tggtcaaact gatattttat
      961 atactgataa caagctgctt ctaaagaatc atttaatgcc atttcaatat tctttttaaa
     1021 cggtggttcc ccaggctgat cccaagctag tttatcagca ataaaaataa tcatatctaa
     1081 tttactagga ttagatctta gtgttgtatg tgattcgatt gcctttaaaa ttgataaatt
     1141 cctaatttga aaatactcat aagctattaa agcagatatt ttttgatgca ataaaaatgg
     1201 atatatcttt tctgcactat ctaattgcca ttgtaattca ttagcaattt tcatcatatc
     1261 ttggggagta ataacagcac taatatcatg taataaagct gctacaatac aagctctaat
     1321 atcttcttga tattgcaaag ctaatttttc tgctaccata gccactccaa gaacatgctg
     1381 atatgttctt tctttattat taagcaaata aaaatctttt atttgttctt caatagttaa
     1441 atccatcata gttacctttc catttaatag ccaatattaa ttaacaagtt atatcttcta
     1501 aaactgtttt taattgcctt actccatttg attgagacgt tataatagct tgtaaaattg
     1561 tttttaattc agaacaaatt ggatattcca aagttgtttt cgccattgca atagcacctt
     1621 catgatgtgg aatcatttca ttaataaaat ttacatcaat gtcattactg catgatggct
     1681 cagccatttt tgtgaacata gttcctacga taatatcaag cctattttga taatcataca
     1741 actgttcttg agaatttgtc tgattttgac aatcacataa aattctccgc atattctcaa
     1801 tacttcttgt ttgttctttt ataatgttaa aagcaatatt ttgtaaagct tcattcttag
     1861 tgtattttaa taaattacat gacattaaaa ttgccgcacg atgatgcggg atcatttgaa
     1921 ccataaaatt ataagatatg ctgcacgaca attcaacact agtcatttta ttaatcattt
     1981 gatttaaaat acagtgatac tcatctaaat aggattgaag tttttgttca taacaataca
     2041 aattttccat ctgctcatct cctatttatt tatatgcatc atgagcatca aatgttcctt
     2101 ttatataaaa caaaaaaagc cccaaaccat tggtttagag ccatttttaa tcaatggcgt
     2161 ccacgaaggg attcgaaccc ctgaccgcac gcttagaagg cgtgtgctct atccagctga
     2221 gctacgtaga cctctctctt gactgctcat atataatagc atatctaaaa gagatgtgca
     2281 agcatttttt aacaaaatat ttgtttaaat ttaacttcta tttttccctg gtcaactaga
     2341 atagtacaat aagattttcc actcccacga cgaggcaaag tggttgaacc aggattaatt
     2401 aaataaaaac ccgtcacctc atcacattga ggaacatggg tatgtcctga taataataca
     2461 tcacaaccat gtctcagcaa atcatctacc atcgcttctt cacgtttata atatccataa
     2521 cgatgcccat gagtaactaa aaatcttact ccttcaactt caaaaacaac ttcatcatca
     2581 aaaggagcca tccaatcgtt gtttccacga acacatatcc agccttttaa ctgctcttca
     2641 cgtgcttcac tatcaccaca atgaacatag taatcgccat caggttctaa acgttgtact
     2701 tcatcgatca tcttatgata accatggcta tcggacatta ctactacttt cttcatattt
     2761 atcacctgac attattatac acaattaagg ttcaaagtta aattacattt taattcggtt
     2821 gtctacttca ccatttacat cttgaactgg gtctatctct tgattatcta caaataatga
     2881 aactttttta acattttcta aatgactagc tgatttaacg attcgattat acaaagtatt
     2941 atcaatcgat tcattatcat ttaaaatatt agcagctaaa tgaattgata gtgttccatc
     3001 ttgtaacgaa cattgatcaa ctaatgataa tggtttatca taatccattt catttaacat
     3061 gattgatact tttgtatcaa tatcattttc atttgtttca attcgtttag ttacaggaac
     3121 ataatattct ttgttattga tagtctctgt attataaaca acaactggtg ttgttttata
     3181 aatataatta gtgcttgctt caaaattatt gatacccaac tgatttgtaa tacagcttgc
     3241 tggtactgta ctattcggta aagtactaat atcattacca tcaattttta aatttacttt
     3301 ttcaatatct ccgttacaaa aaacataaga aaacatttca aaaatatcaa gtgcttcctg
     3361 attactgtta acatacaaat tatcacttaa atcaaaggtt aatgatttat cattaattgc
     3421 catggcattc acttgcaggt tcgaatctaa gataggatgt aaacctaaat actcgtaatc
     3481 attagacttc attacctcaa tcatattacg atattttgta tcatcttcaa cttcagcacc
     3541 aaaatctact tcaattggaa ctaatgtgtt tttatcgtct ctaaatacaa ctgtttgata
     3601 atttgctgtc atcgtagcag tattcttttt ttcattccta ttattatctt gtttaataca
     3661 gaaaaagcat accatcaaac aagcacaaat gataatacca ttacgtagtt ttcttttcat
     3721 ccaatcacct caatataata atatgaagtg atcctcttaa aagaccaaat aaatattata
     3781 tatttcttat ttaataaaaa tactttcatc aaaaaaatta gtcggattat aaaatcacga
     3841 ctaactattt tataataata tgttcaacaa ccaaatctgt ataatcaaaa aaacctgttg
     3901 atgattttaa aaattcatca actctacctg ttgtataaat cttaataaat ttatttttat
     3961 taagatttaa tagattattt ttaattaaaa catctcgaac atttttacaa acagcatcac
     4021 tgctggaaat atattcaata tctggtaaaa tatcttttat ttgattctct aaaatgggat
     4081 agtgcgtaca acctaaaata attgatttaa tctttcctgc ataagaatcc aaatactcat
     4141 gcaatacatc ataaatatct tttttatcca ttccagcctc cactaaagga actaacttag
     4201 gtgttggcaa cgtaaacacc tcaattccag tctgataaca atcaatcgta tctggatact
     4261 tatgagaatt aatcgttgct gctgttgcta taacaagagt attatcgact ccgcgactga
     4321 taaaatcttc cactgttgca tcaatcactc caatgatcaa gacttcctta taaacagctt
     4381 gcagttcatt taagaccgta gcactagtag tattacatgc caagacaatc aatttgacat
     4441 tttctttaat aaaatactca actatttcac ttgcataact aaatagttgt tctcgtgttt
     4501 tttcaccata agggcaatga taattatcac caatataaat aatttcatta ggcagcaagg
     4561 tcctgataga attcaagacc gttagaccgc ctacaccgga atcaaatacc ccaatcgctc
     4621 tttccatagt tactcctcgt ttgcgttata ttcttctaaa gctaaatttt gaacatgtaa
     4681 tttagaagca atttctttac ctacatacct aaatgttgta gagctatatt catgatttgt
     4741 cacatcaact ttatcacttg gataccgcag tataaaacca tattcatggc aatgattgat
     4801 caaccactta tatatgtcag tttgatcaaa atcagcaata cttatttccc ttttttgaag
     4861 atttatagtg gtcccaagct gatattcgtt atagcccggt aagattccgc tactacctaa
     4921 agatgccaca tcatagctga tataagccgc tgttaaaacc atatcattat atccctcatc
     4981 attaagtgcc cgatacatct ccattaaact attatttgca tcttcttgaa gatacataga
     5041 atatttgact cgaggtattc ctcgtatctc aacctgattt ttcggttcat aaccacctat
     5101 atatgtctcg ttattgacaa ccaaagctaa atcacttggg ttataaattc gtttggcatt
     5161 aggctgcaaa tcatgattaa agagcgatgc taatgaaccc ttagtaaaat tattactcag
     5221 caatttaaca ttatcatata attttttacc ttctaaacca tcaaactctt tcataattga
     5281 taaataagta ttagtatctg caatgtatgt ataatcaccc tcatcataaa gtgaccgtaa
     5341 caattggtaa taatcaatat tatcgaaaga aaaaccttct tgattaatat aagcattgac
     5401 taaatcattt tttattagtg tattgcatct tgatagtgca ttactagcaa aagaaacttc
     5461 caatttactt gccagctgat taccaacatt gatcaaatca ctatagtttg aatatttatt
     5521 tgttctattt aacgcattat aaaattcata atactgtaat ttaaactccg gaaattctat
     5581 atatttaata aactgatcga ctgcaatcgc attttccact aagtaatctt gttcttcatc
     5641 atctaaatac atctctatta aagcgcgatt nnnnnnnnnn taggacatat tattcttaaa
     5701 ttttctaaac gattatcatt attaattcca ttaacccgat aaaagcgatc atacttgcgg
     5761 tttattgaaa taaaagcaat cgtaaaacaa agtgttacaa ttaaatataa atgccatctt
     5821 ttcaatttca taatatcacc ttgttccatt atataccaac tagtattaaa aaaccataat
     5881 aaaaggaact agattatagt tctaattcct taaatttaat caattcaaaa actgcttgaa
     5941 tccttgaatc
//
LOCUS       DAOMTA010000080         5892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_256_length_5892_cov_2.390269, whole genome shotgun sequence.
ACCESSION   DAOMTA010000080 DAOMTA010000000
VERSION     DAOMTA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_256_length_5892_cov_2.390269"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLK68_03390"
     CDS             <1..1209
                     /locus_tag="PLK68_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM89487.1"
                     /translation="AHAFFMVGGTTSAVQSMIMASVKAGEKIIMPRNVHRSAINAMIL
                     TGAVPIYVNPDVDKRLGISLGMSLDQVEQVIKDNPDAKAILVNNPTYYGICSNLKAIV
                     ALAHKHNMLALVDEAHGTHFYFNDDLPMSAMEAGADMASVSMHKSGGSLTQSSFLLLG
                     ENVNADYVRQVVNLTQTTSGSYLLMASLDMSRKNLAIHGNEIFNRVRRLAGYARTEIN
                     QIGDYYAYCKELINGDSVYDFDVTKLSIFTLDIGLAGIEVYDLLRDEYGIQIEFGDIG
                     NILAYISVGDSEANIERLIGALLEIRRRFKKDRTGMFDHEYINPEVVISPQAAFYGEK
                     ESLPLYQSIDRVCSEFVMCYPPGIPILAPGERITKEILDYIQYAKEKGCFMTGPEDMA
                     INKLNVLKGR"
     gene            1215..2066
                     /gene="speE"
                     /locus_tag="PLK68_03395"
     CDS             1215..2066
                     /gene="speE"
                     /locus_tag="PLK68_03395"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRM89488.1"
                     /translation="MELWFTERHTNGVNFSIKVDCQLFSGQSEFQKIDIFDSKEFGRF
                     LALDGYMMLTEKDEFIYHEMIVHVPMAVHPEVKKVLVIGAGDGGVIRELCRYETIEKI
                     DMVEIDELVVEVSKKYLPMTACCFDDPRLQIYYQDGLKFVRGKENEYDLIIVDSTDPF
                     GPGEGLFTKEFYGNCYKALNDTGIMVNQHESPFYQEDAVAMQRAHKRIVESFPISRVY
                     QAHIPTYPSGHWLFGFASKKYHPIKDFQKTKWNARGMKTKYYNTGIHVGSFALPNYVE
                     ELLRDVE"
     gene            2056..2919
                     /gene="speB"
                     /locus_tag="PLK68_03400"
     CDS             2056..2919
                     /gene="speB"
                     /locus_tag="PLK68_03400"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRM89489.1"
                     /translation="MWNKNIQTFIGLEASFDEAECVIFGAPMDSTTSYRPGTRFASSS
                     MRQESFGLETYSPYQDKDLEDIKVFDGGDLELPFGNPRKALDIIKVTTKTIIKANKLP
                     CMIGGEHLVTLGAFEAVFEKYPEIRVIHFDAHTDLRDEYLGEKLSHASVIRRIYDLIG
                     DNKIYQFGIRSGEREEFYFAREHTNLNKFNFTGLSKAIAACRGYPVYFTIDLDVLDPS
                     VFPGTGTPEAGGVTFMELLEAMIVVSELNVVAMDINELSPVYDQSGGSTAVACKVLRE
                     LLLAMNKGENK"
     gene            2916..>3999
                     /locus_tag="PLK68_03405"
     CDS             2916..>3999
                     /locus_tag="PLK68_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRM89490.1"
                     /translation="MKRTLIIGCGGVATVAIHKCCQNSEIFEEIMIASRTKSKCDKLK
                     AQLDGKTKTIIHTAQVDADNVEELVALMKDFQPEVVLNLALPYQDLKIMDACLEAGCH
                     YVDTANYEPEDTAKFEYSWQWAYREKFEQAGLTAILGCGFDPGVTGVFSAYALKHEFD
                     EINYIDILDCNGGDHGYPFATNFNPEINIREVSANGSYWEDGHWVETKPMEIKREYDF
                     AQVGKKDMYLLHHEELESLGLNIPGIKRIRFFMTFGESYLTHLKCLENVGMTSIEPIE
                     YEGKQIIPLQFLKAVLPDPASLGPRTVGKTNIGCIYQGKKDGQEKKYYLYNVCDHQEC
                     YKEVGSQAVSYTTGVPAMIGTMLLLQG"
     assembly_gap    4000..4028
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4142..5251
                     /gene="nspC"
                     /locus_tag="PLK68_03410"
     CDS             4142..5251
                     /gene="nspC"
                     /locus_tag="PLK68_03410"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRM89491.1"
                     /translation="MKTPYYLIDEQLLEKNLKILHTVKERTGCKILLAQKCFSMFSVY
                     PLIAEYLDGTTASGLYEAKLGYEEMHKENHVFSTAYRDDEIEEIMNICDHVVFNSFNQ
                     WQKYQNLAKQTSTSCGIRINPECSTQIGHEIYDPCAKFSRMGVTLENFKEELLEGIEG
                     LHFHTLCEQNSDDLITTIRAVEEKFGKYLGQMKWLNFGGGHHITRKDYDLEKLIKIIN
                     EIKEKYQVQVYLEPGEAVALNAGFLVSSVLDITHNGMAIAIMDTSAACHMPDVLEMPY
                     RPEIIDSALPNVKTYTYRLGGPTCLAGDIIGDYSFDQPLQVGQQLWFKDMAIYSMVKN
                     NTFNGMNLPSIVLKQKEKLKIIKEFNYCDFKNRLS"
     gene            complement(5269..>5892)
                     /locus_tag="PLK68_03415"
     CDS             complement(5269..>5892)
                     /locus_tag="PLK68_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM89492.1"
                     /translation="IYDLTHNKCRSFSFLEQIEINELLQLALYYRLNIRANFDHKYQM
                     ILNNWLYFLIPLIRIFTKRYPDLTFYKNTAVVPWNKLGKICLLSSPRKLKLFEAAAQT
                     KFPGLYQYYYTNPYCVEVVKAEVNKCYAVKYLCQLNNLNLENVLAFGDSGNDEELLNN
                     AGIGIIMKNGLSSLKKKATYLTFKNNRHEGVLNMLEYLFDDLNKDKN"
BASE COUNT         2028 a          782 c         1204 g         1849 t
ORIGIN      
        1 gctcatgctt tttttatggt aggcggaacg acttcagcag ttcaaagtat gattatggca
       61 agtgtaaaag caggagaaaa aattattatg ccacgtaatg tccatcgtag tgccattaat
      121 gcgatgatct tgactggggc ggttccgatt tatgtaaatc ctgatgttga taaacgatta
      181 ggaatatctc taggtatgtc acttgatcaa gtagagcaag taatcaaaga taatcccgat
      241 gcaaaagcaa tcttagtaaa taatcctacg tattatggga tatgttcaaa tttaaaagcg
      301 attgttgcat tggcacataa gcataatatg cttgctttgg tagatgaagc acatggaaca
      361 cacttttatt tcaatgatga tttaccgatg tcagcgatgg aggctggagc agacatggcg
      421 agtgtcagta tgcataaatc aggtggaagc ttgacgcaaa gctctttctt actgttagga
      481 gaaaatgtaa atgctgatta tgtacgtcag gttgttaatt tgacacaaac aacaagcggt
      541 tcgtatttat tgatggcaag tctagatatg tcacgtaaaa atttagctat acatggtaat
      601 gagattttta acagggttcg tcgccttgcg ggatacgcac gcacagaaat caatcaaatt
      661 ggtgattatt atgcttattg taaggaattg atcaatgggg atagtgttta tgattttgat
      721 gtaacgaaat tatcaatatt tacacttgat atcggattgg ctggaatcga agtatatgat
      781 ttattaagag atgagtatgg gattcaaatt gaatttggtg atattggaaa tattcttgct
      841 tatatctcgg tcggtgatag tgaagcaaat attgagcgtt taatcggagc acttttggaa
      901 atacgccggc ggtttaagaa ggatcggaca ggaatgtttg atcatgaata tattaatcct
      961 gaagtagtta tttctcctca agcagctttt tatggagaaa aagaatcgtt gccgctttat
     1021 caaagtattg atcgagtctg cagtgaattt gttatgtgtt atcctccggg aattccaatt
     1081 ttagcaccgg gtgaaaggat cacgaaagaa attctagatt atattcaata tgctaaagaa
     1141 aaagggtgct ttatgactgg tcctgaagat atggcaataa ataaacttaa tgttttaaag
     1201 gggagatgaa ataaatggaa ttatggttta cggagagaca tacaaatggt gtgaattttt
     1261 cgattaaagt agattgtcag ttatttagtg gtcaaagtga attccagaaa attgatattt
     1321 ttgactcaaa agaatttgga cgatttttag cgctagacgg ttatatgatg ctaacggaaa
     1381 aagatgagtt catttatcat gaaatgattg ttcatgttcc tatggcagtt catcctgaag
     1441 tcaaaaaagt attagtaatt ggagcaggag acgggggtgt aattagagaa ttatgccgtt
     1501 atgaaacgat tgaaaagatt gatatggttg aaatagatga gttagttgtt gaagtaagta
     1561 aaaaatattt accgatgaca gcatgttgtt ttgatgatcc gcgattacaa atctattatc
     1621 aagatgggtt aaagtttgtt agaggtaaag aaaatgaata tgatttgatc attgttgatt
     1681 caacagatcc ttttggaccg ggtgaaggac tatttacaaa ggaattttat ggtaactgtt
     1741 ataaagcatt aaatgataca ggaattatgg ttaatcaaca tgaaagtcct ttttatcaag
     1801 aagatgctgt tgcaatgcaa cgggctcata aacgaattgt tgaaagtttt ccgatttctc
     1861 gagtctatca agcgcatatt cctacctatc cttcagggca ttggttattt ggttttgctt
     1921 caaaaaaata tcatcccatc aaagattttc aaaaaacaaa atggaatgct cgagggatga
     1981 aaacaaagta ttataatacc ggcattcatg taggttcgtt tgcattacca aattatgtag
     2041 aggagttatt aagagatgtg gaataaaaat atacagacgt ttattggact agaggcatcg
     2101 tttgatgaag ctgagtgtgt aatctttgga gccccaatgg atagtaccac ctcatatcga
     2161 ccgggaactc gttttgcttc atcttcaatg cgccaagagt catttggtct agagacatac
     2221 agtccctatc aggataaaga tttagaagat attaaagttt ttgatggtgg tgatcttgaa
     2281 ttaccatttg gaaatccccg caaagcatta gatataatca aagtaacaac aaaaacaatt
     2341 attaaagcaa ataaattacc atgtatgatt ggtggcgaac atcttgttac gttaggagct
     2401 tttgaggcag tatttgagaa atatccagag atacgtgtta ttcatttcga tgctcatacc
     2461 gatctacgtg atgagtattt aggtgaaaaa ctttcccatg cttctgtgat tagacgaata
     2521 tatgatttaa ttggtgataa taaaatatat caatttggaa ttcgtagtgg tgaacgagaa
     2581 gagttttact ttgctagaga acatactaat ttaaataaat ttaattttac aggactatca
     2641 aaagcaattg cggcatgccg cgggtatccg gtgtatttta caattgatct agatgtcttg
     2701 gatcccagtg tatttccagg aacaggaacg cccgaagcag gaggcgtgac ttttatggaa
     2761 ctattagagg cgatgattgt tgtaagtgag ctaaatgtag tagctatgga tattaatgaa
     2821 ttatcacctg tttatgatca aagtggagga tcaacggcag tcgcttgcaa agtattaagg
     2881 gaattattat tagcaatgaa taaaggagaa aataaatgaa aagaacgtta attattggtt
     2941 gtggtggagt tgcaacagta gcgattcaca aatgttgtca aaacagtgag atatttgaag
     3001 aaattatgat tgcgtcaaga acaaaaagta aatgtgataa actaaaagcg cagttagatg
     3061 ggaaaacaaa aacaattatt catacagcac aagttgatgc tgataatgtt gaagagctag
     3121 ttgctttaat gaaagatttc caaccagagg tagttttgaa tcttgcatta ccataccaag
     3181 atttaaaaat tatggatgct tgtttggaag ctggttgtca ttatgtagac accgctaatt
     3241 atgagccgga agatacagca aaatttgagt atagctggca atgggcatac cgtgaaaaat
     3301 ttgaacaggc gggattaaca gcgattcttg gatgtggttt tgatcctggg gtaacaggtg
     3361 tatttagtgc ttacgcttta aagcatgaat ttgatgaaat taattatatt gatattttag
     3421 actgtaatgg tggggatcat ggatatccat ttgcaacaaa ttttaatcca gaaattaata
     3481 ttcgcgaagt atctgccaac ggaagttatt gggaagatgg acattgggta gagacaaaac
     3541 caatggaaat taaacgtgaa tatgattttg cccaagttgg taaaaaagat atgtatttgt
     3601 tgcatcacga agaactggag tcattgggtt taaatattcc gggaatcaaa cgaattagat
     3661 tctttatgac ttttggcgaa agctatttaa cacatctaaa gtgcttagaa aatgtaggaa
     3721 tgacatcgat tgaaccaatt gaatatgaag gtaaacagat tattccattg caatttttaa
     3781 aagcagttct accagatcct gccagcttag gaccacgaac agttggcaaa acaaatattg
     3841 gatgcattta tcaagggaaa aaagacggtc aagaaaaaaa atattattta tataatgtat
     3901 gcgaccatca agaatgttat aaggaagttg ggtctcaagc cgttagttat actacaggag
     3961 ttccggcaat gattggaacg atgctgttat tacaaggtcn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnaa gagagccggt gtttataatg ttgaagaatt tgacccggat ccatttatgg
     4081 aagcattaaa taaatgggga ttgccatgga ttgaagatca taatccggta ttagtagatt
     4141 aatgaagaca ccatattatt taattgatga acaattatta gaaaaaaatt taaaaatact
     4201 tcatacggtt aaagaacgaa ctggttgtaa aattctattg gctcaaaaat gtttttcaat
     4261 gttcagtgtt taccctctaa tagctgaata tttagatggg acaactgcta gtggactata
     4321 tgaagctaag cttggctatg aagagatgca taaagaaaat catgttttta gtaccgccta
     4381 tcgtgatgat gaaattgaag aaatcatgaa tatatgtgac catgtagtct ttaattcatt
     4441 taatcagtgg caaaaatatc aaaatctagc aaaacaaaca agtacttcat gtggaattag
     4501 aatcaatcct gaatgttcaa ctcaaatagg tcatgaaatc tatgatcctt gcgcaaagtt
     4561 ttcacgaatg ggggtaacat tagaaaactt taaggaagaa ctgcttgagg gaatagaggg
     4621 actgcatttt catactctat gtgaacaaaa cagcgatgat ttaataacta ctattagggc
     4681 tgttgaagaa aaatttggta agtatttagg acaaatgaaa tggttgaatt ttggtggtgg
     4741 tcatcatatt actcgtaaag actatgatct tgaaaaatta ataaaaatta ttaatgaaat
     4801 aaaagaaaaa tatcaagtac aagtatattt agagccaggt gaagctgtgg ctttaaatgc
     4861 tggttttcta gtttcgagtg ttctagatat tacccacaat ggtatggcta tcgcaattat
     4921 ggatacttca gcagcttgtc atatgccaga tgtattagaa atgccgtatc gaccagaaat
     4981 tatcgatagt gcactaccaa atgttaaaac atacacatat cgcttaggag gaccaacttg
     5041 tttagctgga gatattattg gtgattattc atttgaccag cctttacagg taggacaaca
     5101 gttatggttt aaagatatgg caatttattc aatggtaaaa aataatactt ttaatgggat
     5161 gaacttacct agtattgttt taaaacaaaa agaaaaactt aaaataatca aagaattcaa
     5221 ttattgtgat tttaaaaatc ggttgtcata aaaaattagg ctgttagcct aatttttatc
     5281 tttattaaga tcatcaaata aatattctaa catatttaag acaccctcat gtcgattgtt
     5341 tttgaaagta agataggtag cttttttctt taatgaggaa agaccatttt tcataataat
     5401 cccaataccg gcattgttta ataattcttc atcattacca ctatctccaa aagctaagac
     5461 attttctaaa tttaaattat ttaactgaca taaatatttt acagcataac atttattgac
     5521 ttctgcttta acaacttcaa cacagtaagg gttggtgtaa taatattgat atagtccagg
     5581 aaattttgtt tgtgcagcag cttcaaaaag ctttaatttt ctaggtgaag aaagtaaaca
     5641 aatttttcca agtttattcc aaggtactac agcagtattt ttataaaaag tgaggtcggg
     5701 ataacgttta gtaaatattc gaatcaaggg gattaaaaag tatagccaat tatttaaaat
     5761 catttgatat ttatgatcaa aattagcacg tatatttaac cggtaataaa gggctaattg
     5821 aagtagttcg ttaatttcta tttgttctaa aaaagaaaaa gatctacact tattgtgagt
     5881 aagatcataa at
//
LOCUS       DAOMTA010000081         5889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_257_length_5889_cov_2.443435, whole genome shotgun sequence.
ACCESSION   DAOMTA010000081 DAOMTA010000000
VERSION     DAOMTA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_5889_cov_2.443435"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLK68_03420"
     CDS             complement(<1..811)
                     /locus_tag="PLK68_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM89493.1"
                     /translation="MKYDFETLVDRSQNGSAKWNGMKDHNPAVAKNIAPLSVADLDLK
                     LAPEIAEGMLEFMQNNPVFGYTNGTAAYYDAVINWMKDKHNYQVEKEWIVLSNGVVPA
                     LSDGVTAFTEENDGVIIFTPVYYPFYRAIELNNRRVQRCPLINHENSYQIDFDKFEEL
                     AKQANTKLLILCNPHNPVGRVWTKEELEKIADICLNNGIIILSDEIHEDLTMPGYTHY
                     PIAVLSEVIGDITVTCTAPSKSFNIAGLQGSNIIIKNKELRAKFIAQQEKKG"
     gene            complement(899..1360)
                     /locus_tag="PLK68_03425"
     CDS             complement(899..1360)
                     /locus_tag="PLK68_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM89494.1"
                     /translation="MDQERFDNMIKVIDESYDLIQEYDSKPRQFGKVMLYPVETHTLA
                     IIGNHPGINASMIAKELKKTLSASSQILKKLEQKGLISRTKNPHNNREYNLYLTVGGR
                     QIFDLHGQFDKKIMKRYFKNLISFSNEEIDIYIKVQRALNKEYAQDNEESL"
     gene            complement(1448..1768)
                     /locus_tag="PLK68_03430"
     CDS             complement(1448..1768)
                     /locus_tag="PLK68_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HRM89495.1"
                     /translation="MDNQNYSNPTKQPILPNENTFNDYDRINDVLLCLKSLLSDYTTF
                     IIECSHQQLANKLIDIQKEVYQIQREVFDLMYSKGWYPLEPETPQKIQKVVQEYTTKE
                     SHLM"
     gene            complement(1761..1961)
                     /locus_tag="PLK68_03435"
     CDS             complement(1761..1961)
                     /locus_tag="PLK68_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89496.1"
                     /translation="MNQPPKMISTKDSGSFNDQLNSLYVLSKKLKAYEESVEDNDIKM
                     TLGRVNSTIKNHYSELFGCLNG"
     gene            complement(2164..2670)
                     /locus_tag="PLK68_03440"
     CDS             complement(2164..2670)
                     /locus_tag="PLK68_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRM89497.1"
                     /translation="MYEKGDLIIYGNQSVCRIENIGVISIGKQPNSRKYYTLNPIFMD
                     GKTYVPIDTQVYMRHLISIEELERLLTRHPKVQKEIIENQNLRQLTDYYKETISQYTC
                     DGLIQLICNLQAKQKNLLLQNKRLSQTDERYKKEAEDLLHQEFAAVLNIPKEAVMAYI
                     ENKIKVAG"
     gene            complement(3253..3948)
                     /locus_tag="PLK68_03445"
     CDS             complement(3253..3948)
                     /locus_tag="PLK68_03445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM89498.1"
                     /translation="MKFYFDVDDTLYDQFIPFKKAFIEIFPKIKTLPIYQIFIKFRKY
                     SDKIFEASQSGQVTINEMYIYRIQAALKEYYLEIDEAHALAFQTKYYVYQQDITTSNA
                     IKDILDLLKDNHIGIGIITNGPACHQMEKLRRLKMSNWVALEDIYISSVVGYSKPDPQ
                     IFNLITDKDCIYVGDSYENDVIGAKNANWKCIWLNKKGLKAMDIKPDYEVNDEEALLN
                     LISKIVKESAQKM"
     gene            complement(3960..>5889)
                     /gene="tkt"
                     /locus_tag="PLK68_03450"
     CDS             complement(3960..>5889)
                     /gene="tkt"
                     /locus_tag="PLK68_03450"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRM89499.1"
                     /translation="LCADTVEKANSGHPGLPLGSAAAAYILWGKVMKHSGTHRKWVNR
                     DRFILSGGHGSSLLYSLLHLYNYGLTIEDLRQFRQLDSLTPGHPEYGHTVGVEATTGP
                     LGAGMAMATGMAMAQAHLAAIFNRPGYPVFDSFTYVLGGDGCLMEGISSEAFSLAGTL
                     KLEKLIVLYDSNNISIEGSTDIAFREDVQGRMKAFGFDTFTVEDGDDLDEILSAINQA
                     RVSQKPAFIEVKTRIGKGCPAKEGKASAHGEPLGEENVRELKRTLGLDEDRTFAIDEE
                     VYLNTAKQQERSEVIYKIWKDMFDDYLVTYPEMKKLWKQYFEVEYNKVLENDVEFWKF
                     ENKPESTRNLSGTAMNRIKELFPNFMGGSADLAPSTKTYLNKVGDFSAENYRGRNLHY
                     GVREQAMTGIGNGIMLYGGLKTFVSTFFVFSDYVKPMARLSALMNLPLIYVLTHDSIG
                     VGEDGPTHEPVEQLTMLRAMPNFNVFRPCDAQETNAGWYLAMTSKTTPTALVLSRQNL
                     PQIKGSSREALKGGYVIDECEGTPEIIIIASGSEVTLAVEAKKRIRGKAIRVVSMPSM
                     DIFKQQSREYRESILPPGIEKRIAIEAGSRMSWGEFVGLKGKYITMDSFGASAPADEL
                     FKRYGFTVDNVVKMINKL"
BASE COUNT         1765 a         1176 c          761 g         2187 t
ORIGIN      
        1 atcctttttt ctcttgttgc gcaataaatt tagctcttaa ttctttattc ttaataataa
       61 tattagatcc ttgcaaacct gcaatattaa aacttttact tggtgctgta catgttacag
      121 taatatctcc aatcacttca cttaaaacag caattggata atgtgtatac cccggcatag
      181 ttaaatcttc atgaatctca tcactaagaa ttataattcc attatttaag caaatatccg
      241 caattttttc tagctcttct tttgtccata cacgccctac aggattatga ggattacaaa
      301 gaatcaataa ctttgtattt gcttgttttg caagttcttc aaatttatca aaatcaattt
      361 ggtaactatt ttcatgattt attaaaggac atctttgtac tcgacgatta tttaactcaa
      421 ttgcccgata gaaaggataa taaactggcg taaaaataat tactccgtca ttttcttcag
      481 taaatgcagt taccccatcg cttaacgctg gtactacacc attagataaa acgatccatt
      541 ctttttcaac ttgataattg tgtttatcct tcatccagtt aattactgca tcgtaataag
      601 ctgctgttcc atttgtatag ccaaatacag gattattctg cataaattca agcatccctt
      661 cagcaatctc tggtgctaat ttcaaatcta aatcagcgac tgaaagtggc gctatatttt
      721 tagcaactgc aggattatga tctttcatcc cgttccattt agcagaacca ttttgactcc
      781 gatcaactaa tgtttcaaaa tcatatttca tttctctacc tccattatta agcacctata
      841 tagttaagta ccttaataat agtatactct cacaatttat aaagtcaaat aaaaagaatc
      901 ataacgattc ttcattgtct tgtgcatatt ctttatttaa agctctttga accttaatat
      961 aaatatctat ttcttcattg ctaaaactaa ttaaattctt aaaataacgt ttcataattt
     1021 tcttatcaaa ctgaccatgt aaatcaaaaa tctgcctccc gccaaccgtt aagtataaat
     1081 tatactcacg attattatga ggattcttgg ttcttgaaat taatcctttt tgttctagct
     1141 tctttaaaat ttgactgctg gcacttaatg ttttttttaa ttctttagca atcatcgaag
     1201 catttattcc agggtgattt ccaattattg caagagtgtg agtttctact ggataaagca
     1261 tcactttgcc aaattgtcga ggtttactat catattcttg tattaaatca taactttcgt
     1321 caattacttt aatcatatta tcaaatcgct cttgatccac aaaaatcacc tcaggtatca
     1381 ttctatcaaa taataaccaa tgttgcaaac aatcataaaa agactggttt taccagtctt
     1441 taaaggatta cattaaatgt gattctttgg ttgtgtattc ttgaactact ttttgaatct
     1501 tttgtggagt ttctggttct aatggatacc agcctttaga atacataaga tcaaagacct
     1561 cacgttgaat ctgatatacc tctttttgaa tatctattaa tttatttgct agttgttgat
     1621 gagagcattc aatgataaaa gtcgtataat cacttaataa tgattttaaa cataatagaa
     1681 catcattgat tctatcataa tcattaaacg tattttcatt tggtaaaatt ggttgtttcg
     1741 ttggatttga ataattttga ttatccattt aaacacccaa ataattcact ataatgattt
     1801 ttaattgtgc tattaacacg tcctaaagtc atcttaatat cattatcttc aacactttct
     1861 tcgtaagcct ttaacttctt cgataataca tataaactat tcaattgatc attaaaagag
     1921 ccactatctt tagtagatat catttttggt ggttgattca taaaattcct ccttcaataa
     1981 tattttggtt cttttataaa acaattatgc ataatcatat acaaaagatt aaaataaaaa
     2041 tattttttat tttctactta taaatttttc aaatctgact aatactagag tatctttatt
     2101 aaaaaataaa aaacattatt atcttcaata atgtttcttt accaaattaa cagccctaaa
     2161 tttttatcct gctactttta ttttattttc aatatatgcc attactgctt ctttagggat
     2221 atttaatacc gctgcaaatt cttgatgcag tagatcttct gcttcttttt tgtaccgctc
     2281 atcagtttga cttaatcgct tattttgtaa caataaattt ttttgctttg cttgaagatt
     2341 acaaatcaat tgaatcaagc catcacaagt atattgactt atcgtttctt tgtaataatc
     2401 tgttaattgt cttaaatttt gattttcaat aatctctttt tgtactttag gatgtctggt
     2461 caataatcgt tctaactcct ctatcgaaat caaatgacgc atatatactt gcgtatcaat
     2521 tggcacatac gtcttaccgt ccataaaaat aggatttaaa gtataatact ttcgggaatt
     2581 gggctgctta ccaatactaa ttactccaat attttcaatc ctacaaacac tttgattacc
     2641 atatataatc aaatctcctt tttcatacac ataaccacct agctttctat taagtatatg
     2701 cttaatatat ctattatacc gcactattag ttactatatg caaaagtaat taaaatatac
     2761 taaattttct tttttatgca gctaaagact acatcttttt atatttgatt ttactattta
     2821 aatcttgtta ataatttcaa cagttttaca tgtttcgtat atcattaggg cttgctttag
     2881 caatgatggt gacagtattt tttagctaaa aacctttatt gttgtaatat cgttaatcca
     2941 actagaattt gttaaaacag taagatagat tgaccatcgt ttaattggta tttcactttc
     3001 cattcaatat tttattccta atgaagatac tattttagtt gaaatattat taggtacttc
     3061 ggtttatttt taccagaata taaatataaa taaattatca atattgacag tgcgctaaat
     3121 atatcgatcg atagttcttt tagatactaa aacagcaata gaaaatcaac ttattagttt
     3181 aatcagtgaa atatttattt catgaccaaa ctaatattat ctactaacta aaaaatgcag
     3241 accattgttt gcctacattt tttgtgcaga ttcttttact attttagaaa tcaaatttaa
     3301 caaagcttct tcgtcattaa cttcataatc aggtttaata tccattgctt taagtccctt
     3361 tttatttaac caaatacatt tccaattagc atttttggcc ccaatcacat cattttcata
     3421 agaatcaccg acatagatac aatctttatc cgtaattaaa ttaaatatct gtggatctgg
     3481 ttttgaataa ccaacaaccg atgaaatgta gatatcctcg agagcaaccc agttgctcat
     3541 ctttagtcta cgtaattttt ccatttgatg acaagcaggt ccattagtaa ttatccctat
     3601 tcctatatga ttatctttta ataaatctaa tatatctttg attgcattac tagttgtaat
     3661 gtcttgttga tatacataat attttgtttg aaatgctaaa gcatgtgctt catcaatttc
     3721 aagataatat tcttttaagg ctgcttggat acgatagata tacatttcat ttattgtaac
     3781 ttgaccagac tggcttgcct caaatatttt atcactatat ttacgaaatt tgataaaaat
     3841 ttgatatata ggcaaagtct ttattttagg aaatatttca ataaatgcct ttttaaatgg
     3901 aatgaactga tcatatagcg tgtcatcaac atcaaaatag aatttcatct ttttaccgtt
     3961 tataatttat taatcatttt tacaacatta tctactgtaa aaccatatct tttaaacagt
     4021 tcatcagcag gtgctgaagc tccgaagcta tccattgtta tatatttgcc ttttaagcca
     4081 acaaactctc cccaggacat tcttgaaccc gcttcaattg cgattctctt ttctatacct
     4141 ggcggcagga tactttctct atattcccgg ctctgctgtt taaatatatc catactcggc
     4201 atacttacta cccttatcgc tttgcctctg atccgtttct ttgcttctac tgccaatgtc
     4261 acttctgatc ctgaggctat gatgattatt tccggtgttc cttcacactc atcaataaca
     4321 tatcctcctt ttaatgcttc tcggctagat cctttgatct gtggtagatt ttgtcttgat
     4381 aaaaccagtg ctgttggtgt tgtttttgat gtcatcgcca gataccatcc agcattagtc
     4441 tcctgtgcat cgcacggtct aaacacattg aagttcggca tcgctctcag catcgtcagc
     4501 tgttctaccg gttcatgagt tggcccgtct tcacctacac cgatactgtc atgcgtcaat
     4561 acatatatca atggcagatt catcaaagct gacaatctag ccatcggttt gacatagtcg
     4621 ctgaatacaa agaacgttga tacaaaagtc ttcaatccac cataaagcat tattccgttt
     4681 ccaatccctg tcatcgcctg ctctcttact ccgtagtgta aattccttcc cctgtaattc
     4741 tcggcactaa agtctcctac tttatttaaa tatgtttttg tagatggtgc taagtctgct
     4801 gaaccaccca tgaaatttgg aaatagttcc tttatccggt tcatcgcagt tcccgacaaa
     4861 tttcttgttg attcaggttt attttcaaat ttccagaatt ccacatcgtt ttcaagtact
     4921 ttattatatt ccacctcaaa atactgcttc cataattttt tcatctctgg atatgttacc
     4981 agatagtcat caaacatatc cttccaaatc ttatagatta cctcgctgcg ttcctgctgt
     5041 tttgctgtat tgagataaac ttcctcatct atcgcaaatg tcctatcctc atcaagtccc
     5101 agtgtccttt ttaattccct gacattttct tcacccagtg gctctccatg tgcactggct
     5161 tttccctctt tagccggaca tccttttcca attctggttt tcacttcaat aaaggccggc
     5221 ttttgagata ccctagcttg atttatcgca cttaatattt cgtcaagatc gtctccatct
     5281 tcaaccgtga atgtatcaaa accaaatgcc ttcattcttc cctgaacatc ttctctaaag
     5341 gcaatatccg tgcttccttc aatcgaaata ttatttgaat cgtataaaac gatcagtttc
     5401 tccagtttta aagtgcctgc aagtgaaaat gcctctgacg agattccttc catcagacag
     5461 ccgtcaccgc ctagtacata agtaaagctg tcaaatactg gatatcccgg tctattaaaa
     5521 atcgctgcca agtgtgcctg ggccattgcc attcctgttg ccatcgccat ccctgctccc
     5581 aatggtcctg tcgttgcctc gacccctaca gtatgtccgt attcaggatg ccccggagtc
     5641 agtgaatcaa gctgtctaaa ctgtcttaga tcctctatcg ttaatccata gttatacaaa
     5701 tgaagcagac tataaagcag tgatgatcca tgtcctcccg acaagataaa gcgatcccgg
     5761 ttgacccact tccgatgtgt tccactatgt ttcatgacct ttccccataa gatataggcc
     5821 gccgccgctg aacccaaagg cagtcccgga tgtcctgaat ttgccttttc aacagtatca
     5881 gcgcataat
//
LOCUS       DAOMTA010000082         5766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_269_length_5766_cov_1.959377, whole genome shotgun sequence.
ACCESSION   DAOMTA010000082 DAOMTA010000000
VERSION     DAOMTA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_5766_cov_1.959377"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="dnaB"
                     /locus_tag="PLK68_03455"
     CDS             <1..900
                     /gene="dnaB"
                     /locus_tag="PLK68_03455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRM89500.1"
                     /translation="RGVIKSVTDRLNLLQKIDGNISGVKSGFRDLDKITSGFQKGDLI
                     ILAARPAMGKTAFALNLAHNAAYKAEEPVAIFSLEMPAEQLVQRVICSMGGIEGSSMR
                     TGEILKTNANKYYAAAERVSKCNMYIDDSPGIKINDIVAKSRKLKSEHGLRMIVIDYL
                     QLITTASKNKENRQQEVSEISRTLKALARELEVPVISLSQLSRSVEQRPNKRPMMSDL
                     RESGAIEQDADIVSFIYREDYYKDPGEESEDNGLTEIIIAKHRNGATGEVNLAFEKNY
                     SRFSDLAQMGPDGTSEGVRDLRS"
     gene            912..1502
                     /locus_tag="PLK68_03460"
     CDS             912..1502
                     /locus_tag="PLK68_03460"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRM89501.1"
                     /translation="MYHQYREGWIEVISGCMFAGKTEELIRRINVLSYAKKNIIVFKP
                     KIDNRYSDSEIVSHSGAKVPCLVVEKAQDILKKIEADTEVVAIDEVQFFDKDIVEVCE
                     YLADKGIRVMVAGLDKDFRGESFGVMPELLTRAEFVTKLTAVCAKCGAPATRTQRLVN
                     GKPAGFEDPIVMVGADESYEPRCRHCHQVPNKPHKF"
     gene            1866..2954
                     /gene="prfA"
                     /locus_tag="PLK68_03465"
     CDS             1866..2954
                     /gene="prfA"
                     /locus_tag="PLK68_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRM89502.1"
                     /translation="MNESMLDRLKTMENRYEELGHMLMDPDIGSDIKKMTEVTKEQSS
                     LQAAYDLYQEYKEVEAGISDAKELAKESDPEIKEMAKMELAELETRLPEIISKLEIEL
                     IPKDPNDNKDVIMEIRGAAGGDEGNIFAGDLYRMYVKYAESQGWKVEVMEAVDAEAGG
                     YSLISFMVKGEDVYGKLKFESGSHRVQRVPKTETQGRVHTSTATVLVMPEMEEVDVEI
                     NKSDLRIDTYRASGAGGQHINKTDSAVRITHLPTGIVAASQDGRSQHDNKDKAMKALV
                     SRIYDFYQQQHDEQVGSERKSKVGSGDRAEKIRTYNYPQNRVTDHRIGLTIQQLDRIV
                     DGKLDDIITALINEDQRLKMEGQHSWQL"
     gene            2942..3799
                     /gene="prmC"
                     /locus_tag="PLK68_03470"
     CDS             2942..3799
                     /gene="prmC"
                     /locus_tag="PLK68_03470"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRM89503.1"
                     /translation="MATVKELIKLAESRLDDASKDVNVAKVLFYHLADKQPHELYLMY
                     DEEVSSELEAKFLAGMEEYYQGKPIQYIKGVENFFGRDFKVNEDVLIPRYETEELVEN
                     ILYRIDDYFAEYQSITLCDVGTGSGAIATSLALEEPRLKVFATDISLKAVTVAKDNAK
                     NLGANIEFMVGDMLEPLLENEIKVDIFVSNPPYIPQEQEIEAMVKDNEPHVALFGGND
                     GLYFYRKIFQGVEPLLQERALLAFEMGFDQRELMEAALQEYFPNDPHEIIKDINGKDR
                     MLFIYRNLK"
     gene            3847..4596
                     /locus_tag="PLK68_03475"
     CDS             3847..4596
                     /locus_tag="PLK68_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89504.1"
                     /translation="MIINKLNDIINNSQTSDPRYMISSFIKQNILIINSLTIKEVADG
                     SHVSKAMVSKFVKELGYDNFAELKESCSMYVGSLGLKDRYFQLDSDFRSNSSDLVVRM
                     NNLLTNTVHQINYNDLDMLVEDIRNCSCLFLLGHGEAKGLCSMIQIELDALQIPIIVV
                     DVDFRKEYRIGDHAVFLIISVNGNTLMYNHRTITKILKQKQKTWLITCNQEIAFAGKK
                     IYVPSVDSTLNKINIRFVTDLIIARLQQNQE"
     gene            4684..>5766
                     /locus_tag="PLK68_03480"
     CDS             4684..>5766
                     /locus_tag="PLK68_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM89505.1"
                     /translation="MAFSKDFLWGGATAANQCEGGYNEGGRGLANVDVVPHGKDRFPV
                     CLGMKEMLDFEEGYYYPAQVGIDFYHHYQEDIKLFAEMGFKTFRLSIGWTRIFPNGDE
                     NEPNEEGLKFYENVFNECHKYGIEPLVTITHFDMPIHLIKKYGGWKNRELIEMYKKLV
                     TVLFTRYKGLVKYWLTFNEINMILHMPFMGAGLMFKEGEDQKEAKYIAAHNELVASAW
                     ATKIAHEIDSNNMVGCMLAGGEYYAYSCHPADVWASINKNRENIMFIDVQARGYYPNY
                     ALKMFEREGINIGITTEDKEILKNNPVDFISFSYYSSRCISTQGNVEKTSGNAFEGTK
                     NPYLKTSEWGWAIDPLGLRITLNTLYD"
BASE COUNT         2016 a          727 c         1222 g         1801 t
ORIGIN      
        1 cgaggggtta ttaaaagtgt tacggatcgt ttaaatttac tccaaaaaat tgatggaaat
       61 atatctgggg taaaatctgg gttccgtgat cttgataaga ttacatctgg ttttcaaaag
      121 ggagatctaa ttattttggc tgcacgtcca gcgatgggga aaacagcctt tgccttgaat
      181 ctagcccata atgcagcgta taaagctgag gaaccggttg ctattttttc cctggaaatg
      241 cctgctgaac agcttgttca gagggttatc tgttcaatgg ggggaataga aggttcttct
      301 atgagaacag gtgaaatttt aaaaacaaat gcaaataaat attatgccgc agcagaacga
      361 gtatctaaat gtaatatgta catagatgat agtccgggta ttaaaatcaa tgatattgtt
      421 gcaaaaagtc gtaaattaaa aagtgagcat ggcttaagga tgattgtaat cgattatttg
      481 caattgatta caactgcttc taaaaataaa gaaaatcgac aacaagaagt atctgagatt
      541 tcaagaacgc taaaggcatt agccagagaa cttgaagtac cagttatttc attatcacaa
      601 ttatcacgtt cagttgaaca acgacctaat aaacggccga tgatgtctga cttaagagag
      661 tctggggcaa tcgagcagga tgccgatatt gtttcgttta tttatcgtga agattattat
      721 aaagatcctg gagaagaatc agaagataat ggattaacag aaatcattat tgcaaaacat
      781 cgtaatgggg caacaggtga agttaatctt gcttttgaaa agaattatag ccgtttttct
      841 gatttggctc aaatgggtcc agacggaaca agtgaaggcg tgcgtgatct tagaagttaa
      901 ggaggaatta gatgtatcat caatatcgtg aaggctggat tgaagtaatt agtggatgta
      961 tgtttgcagg aaaaacagaa gagttgattc gacgcatcaa tgtattaagc tatgctaaga
     1021 agaatattat tgtgtttaag cctaaaatag ataatcgtta ttctgatagt gagatagttt
     1081 ctcattctgg tgctaaagta ccttgtttag tagttgaaaa agcccaggat atcttaaaga
     1141 aaattgaagc tgataccgaa gtagtagcaa tagatgaagt gcagtttttt gataaagata
     1201 tcgttgaagt ttgtgaatat ttagctgata agggaattcg cgtaatggtt gcaggcttag
     1261 ataaagattt tagaggtgag tcatttggcg tgatgcctga attattgact cgggcagagt
     1321 ttgtcacaaa gttaacagcg gtttgtgcta aatgtggggc cccggcgaca agaacacaac
     1381 gtctagttaa tggtaaacca gcaggatttg aagaccctat cgtgatggtt ggggctgatg
     1441 aaagttatga accaagatgt cgtcattgcc atcaagttcc taataaaccg cataaattct
     1501 aattatgaga gatttttata gtttgggtta taaaaatata aagaagcagg ttttaatttg
     1561 gttttagtgc tgtcaagtag acacactggt taaatttaac cttcttttaa aaagtacggc
     1621 ctaaagcaaa ataaagacaa gttacgttag taacttgtct tcgtttttat gattaaaggg
     1681 agggtagttt tcttactaca ctaatatcat aaaatagcta ggtgaactga atgtgaacta
     1741 aataataata ttagaaatat ttaaaagttt taaaatgtaa ataaaagacg atgaattcaa
     1801 acaaaattta ttttgcttat ttagtatgtt tattatataa tatgagaggt ttggaggtat
     1861 aaatcatgaa tgagagtatg ttagatcgtt taaaaacaat ggagaataga tatgaagaat
     1921 taggacatat gctgatggat ccagatatcg gtagcgatat taagaaaatg acggaagtta
     1981 caaaagaaca gtcatcactt caagcagcat atgatttata tcaagagtat aaagaagtag
     2041 aagctgggat cagcgatgct aaagaattag ctaaagaaag tgatcctgaa attaaagaaa
     2101 tggctaaaat ggagttagcg gagttggaaa cacgcttacc tgagattata agcaagttag
     2161 aaattgaatt aattccaaag gatcccaatg ataataaaga tgtaattatg gagattcgtg
     2221 gtgctgctgg cggtgatgaa ggaaatatct ttgcaggaga tttatatcgg atgtatgtaa
     2281 aatatgcaga gtctcaaggt tggaaagttg aagtaatgga agctgttgat gccgaagcag
     2341 gtggctattc attaatttct ttcatggtaa agggtgaaga tgtttatggt aaattaaaat
     2401 ttgaatctgg atctcaccga gtacaacggg ttcctaaaac cgaaacgcaa ggacgtgtcc
     2461 acacttcaac agcaactgtt ctagtaatgc cggaaatgga agaagtagat gttgaaatta
     2521 ataaaagtga tttaagaatt gatacgtatc gtgctagcgg tgcaggaggt caacatatta
     2581 ataagactga ttctgcagta aggattaccc atcttccaac tggaattgtg gcagctagtc
     2641 aagatggacg ttcacaacac gataataaag ataaagcaat gaaagcttta gtttcacgaa
     2701 tttatgattt ttatcagcaa caacatgacg aacaagttgg tagtgaaaga aagagtaaag
     2761 ttgggtctgg tgatcgtgct gaaaaaatca gaacatataa ttatcctcaa aatcgagtaa
     2821 ctgatcatcg tatcggttta acgatccaac aattagatcg tattgttgat ggaaaattag
     2881 atgatattat tacagctttg attaatgaag accaacgttt aaaaatggaa ggtcaacatt
     2941 catggcaact gtaaaagagc tgattaaatt agcagaaagc agattagatg atgctagtaa
     3001 agatgttaat gttgctaaag tgctgtttta tcaccttgct gataaacaac cgcatgagtt
     3061 atatttaatg tatgatgaag aagtcagttc agaactagaa gccaagttct tggcaggtat
     3121 ggaagaatat tatcaaggaa aaccaattca gtatataaaa ggcgtagaaa acttttttgg
     3181 acgtgatttc aaagttaatg aagacgtttt gattccacgt tatgagactg aggaattagt
     3241 ggaaaatatt ttatatcgta ttgatgacta ttttgctgaa tatcaaagca ttactctttg
     3301 tgatgttgga acgggtagtg gggcgattgc aactagtctt gctttagaag aaccgcgtct
     3361 aaaagttttc gcaactgata tttctttaaa agcagttaca gtcgctaaag ataatgcaaa
     3421 gaatctgggt gctaatattg aatttatggt tggagatatg ttagagccat tattagaaaa
     3481 tgaaataaag gttgatattt ttgtttctaa tcccccatat attcctcaag aacaagaaat
     3541 cgaagcaatg gttaaagata atgaaccaca tgtggcttta tttggtggta atgatggact
     3601 atatttttat cgtaaaatat ttcagggagt cgagccatta ttgcaggaac gggctttatt
     3661 agcatttgaa atgggatttg atcaacggga gcttatggaa gcagctcttc aagaatattt
     3721 tcctaacgat ccccatgaaa tcattaagga tattaatgga aaagatcgta tgttatttat
     3781 ttatcgtaat cttaaataag caccgaaagg tgcttttgtt gtataatgaa ataaaggtgg
     3841 gatatgatga tcattaataa gttaaacgat attatcaata atagtcaaac gagtgatcct
     3901 agatatatga tctctagctt tattaaacaa aatattctta taattaattc cttaactatt
     3961 aaggaagtag ctgatggaag tcatgtttct aaagccatgg tctcgaagtt cgttaaagaa
     4021 ttaggttatg ataactttgc tgagttaaaa gaaagttgtt cgatgtatgt cggaagtctt
     4081 ggattaaagg atcgatattt tcagttagat agtgatttta gaagtaatag ttcagattta
     4141 gttgtgcgaa tgaataattt attaaccaat acagtacatc agatcaatta taatgatcta
     4201 gatatgctcg tagaagatat tagaaattgt tcttgcctat ttttgttagg tcatggagag
     4261 gcaaaaggac tatgttcaat gatccaaatt gaattggatg cacttcaaat accgattatt
     4321 gtagtagatg ttgattttag aaaagaatat cgaattggtg atcacgctgt atttttaatt
     4381 atcagtgtta atggtaatac actaatgtat aatcaccgaa caattactaa aatattaaag
     4441 caaaaacaaa agacttggtt aataacttgc aatcaagaaa ttgcttttgc tgggaaaaaa
     4501 atttatgttc cctctgttga ttctacattg aataagataa atatccgctt tgtgacagat
     4561 ttgattattg ccagattgca acaaaaccaa gagtaaatat tttagttcac agtttgtgaa
     4621 ctattaagta tttattcttt ttttatagta aggtaaggat atagtaaaaa ggaggaatga
     4681 aagatggctt tttcaaaaga ttttttatgg ggtggagcaa ctgctgctaa ccaatgtgaa
     4741 ggtggatata atgaaggggg acgtggtctt gctaatgttg atgtagtacc tcatggtaaa
     4801 gaccgttttc ctgtttgctt aggaatgaaa gaaatgctag attttgaaga gggatactat
     4861 tatcccgctc aagttggaat tgatttttat catcattatc aagaagacat taaactattt
     4921 gctgaaatgg ggtttaaaac atttagacta tcgatagggt ggactcgtat ttttcctaac
     4981 ggtgatgaaa atgaaccgaa tgaagaaggc ctaaagtttt atgaaaatgt ttttaatgaa
     5041 tgtcataaat atggaataga gcccttagta acgattactc attttgatat gcctattcat
     5101 ttaattaaga agtacggcgg atggaaaaat cgtgaattaa ttgaaatgta taaaaaatta
     5161 gttactgttc tttttacaag atataaaggt ctagtaaaat attggttaac ttttaatgaa
     5221 atcaatatga tcttacatat gccatttatg ggggcaggac tgatgtttaa agaaggcgaa
     5281 gatcaaaaag aagctaaata tattgcggct cataatgaat tagtagcaag cgcttgggct
     5341 accaagattg ctcatgaaat tgattctaat aatatggtag gatgtatgct ggctggaggc
     5401 gaatattatg catatagttg ccatcctgca gatgtttggg cttctattaa taaaaatcgg
     5461 gaaaatataa tgtttattga tgttcaagca cgtggatatt atccaaacta tgctttaaaa
     5521 atgtttgaac gtgaaggtat caatattggt attaccacag aagataaaga aattttaaag
     5581 aataatccag ttgactttat ttcttttagt tattattcat cacgttgtat ttcaacacaa
     5641 ggaaatgttg aaaaaacttc tggaaatgct tttgagggaa ctaaaaaccc atatttaaaa
     5701 actagtgaat ggggttgggc aatcgatccg ttaggattaa gaatcacatt aaatacttta
     5761 tatgat
//
LOCUS       DAOMTA010000083         5752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_270_length_5752_cov_2.557135, whole genome shotgun sequence.
ACCESSION   DAOMTA010000083 DAOMTA010000000
VERSION     DAOMTA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_5752_cov_2.557135"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>317
                     /locus_tag="PLK68_03485"
     CDS             <1..>317
                     /locus_tag="PLK68_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRM89506.1"
                     /translation="GFKRNNQLLSLCGLNCGLCPMHIDGYCPGCGGGAGNQSCRIARC
                     SLEHDNLTYCYECSSYPCDKYMKFAKYDSFITHQHYHQDLMKAKDGGIDTYNQEQLEK
                     IDI"
     assembly_gap    318..327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <328..530
                     /locus_tag="PLK68_03490"
     CDS             <328..530
                     /locus_tag="PLK68_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRM89507.1"
                     /translation="RKKSFYCLAVNLLELEELKSLIKQIETNSKLSALTKKEQAKYVF
                     DLFQDLAKQHSLILKLNKKSKK"
     gene            complement(574..966)
                     /locus_tag="PLK68_03495"
     CDS             complement(574..966)
                     /locus_tag="PLK68_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM89508.1"
                     /translation="MIINLDYTSEIPIYLQLRNEIVKGIANGQFKYGESLPTVRTLAQ
                     DLQVNNMTVNKAYGLLKQEGYICIDRRHGAKVQPTIDNSHEHQEKLLDELELLASEAI
                     VKGMNKDEFINTCKALLQTIQYDASIVQ"
     gene            complement(963..2336)
                     /locus_tag="PLK68_03500"
     CDS             complement(963..2336)
                     /locus_tag="PLK68_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRM89509.1"
                     /translation="MWILILCLVFINVVMYFSSKRESRYNEGRLFLVTIPDWTYESVE
                     IKEIEKQFKKEHLWVFIISFITFIPLFIFSTKWLLLYFMIVIWFNVSVYYVPYRKARA
                     KLLALKKLRNWPDEKIEKIKIDLSLSAYMEKHPFNLRRYFFVLIIDLSVLGNMIYFHA
                     ENAMYLYMVLQFMVLVLGIVFIKKLPNKTFCKNSEVNITLNLLRRDSFHHCFFFLITG
                     DSIFNLALQFFLLEKLPFVILFLVALIMILCVIIIVIKANHYREKKAKILAHYNECEY
                     TISNDDCWKIGWFGPTYYNKADPRTLISAPNGTQMTFNTAKPAYRIFIIGIWTFVIAL
                     LLWLFGYPYYLDITNNLVNLSLTDQAVVVDSPFYDVSIDLQKVNKAELADDLGKGIRT
                     NGTDTFVYGKGNYTFDRYGKCKVYMASLHPCYIILYTDDITYIVNDDDIQNTKLIYQE
                     IQEVLSQ"
     gene            complement(2474..2716)
                     /locus_tag="PLK68_03505"
     CDS             complement(2474..2716)
                     /locus_tag="PLK68_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM89510.1"
                     /translation="MFLFVSTLIAYFATAVGLVGHTYIVIPWVTPPLLSGYLSTGDIR
                     GTPSQIVITIIGLLVYAPLVSASNKAAKKQGLETAE"
     gene            2795..3196
                     /locus_tag="PLK68_03510"
     CDS             2795..3196
                     /locus_tag="PLK68_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89511.1"
                     /translation="MRPHPNGQSLFLEKINTVTTKINKIIKKNETFVSQYNDDFKDAV
                     RFSVIDYGATVGTAKEMETKFSETIRVASQGYKLEAYMHGPYLEVNPEHRIFFIETPS
                     PRLSKAKLLKAYESQYTEHVFAITKAQSIRP"
     gene            3462..3923
                     /locus_tag="PLK68_03515"
     CDS             3462..3923
                     /locus_tag="PLK68_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM89512.1"
                     /translation="MLGEKLMRLRKKQGYSQQEVADKLSVSRQTISNWECDQALPAVD
                     KAMELAQLYNISLDDLMENEIEIVSNNKTKDLHLLQYLIGKTCTLECTRDAYLLDIST
                     SDGKVLIVDVNDDWVKVQYHRTKKGSFIKKETVTKLIDLSCISGFKVEGEL"
     gene            3923..4252
                     /locus_tag="PLK68_03520"
     CDS             3923..4252
                     /locus_tag="PLK68_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89513.1"
                     /translation="MTTIIAIIALVISICAIYEIEKLKKNDVKNIEKEIKETDRIKNI
                     LAGLKQCDCELIVKETMFLIDVPYSVEGKIIDLDDDWVLIVSMGRKTISRMIRITMIK
                     DVIELTK"
     gene            4347..5309
                     /locus_tag="PLK68_03525"
     CDS             4347..5309
                     /locus_tag="PLK68_03525"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRM89514.1"
                     /translation="MSVKQNVIALLEENRSKVISGQELANQLYVSRAAIWKAIKTLKE
                     EGYNIEATPNKGYVLLENSDVLSKQGIAYYLTEEIDIFSYKTIDSTNTQMKKLAINGG
                     KNHSVIVSEEQSAGRGRFGRSFYSPAQKGVYMSVLLKTGDSLQNATMITIKTAVAVRR
                     AIAKLYDIEVAIKWVNDLYYRGKKVCGILSEAISDFESGMIEAIIIGIGINVSTDNFP
                     LEIASIATSLGLQEANRNQFIAEILNQLFAIIDEDFKLVLNEYRMASCVLHKQITFNQ
                     KGEQFTGLVREINDLGNLVVSSNGAEMVLTAGEVSIIGGNHGAE"
     gene            5296..>5752
                     /locus_tag="PLK68_03530"
     CDS             5296..>5752
                     /locus_tag="PLK68_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM89515.1"
                     /translation="MEQNKLLTTKKITYCAIFTALITIGAFIQIPVPFMDYFTLQFFF
                     VLLAGILLGSKLGALAVLLYVVIGLLGLPIFAAGGGLAYIVRPSFGYLIGFIAGAYVT
                     GIICEKTNEIAAKKYVLAVLSGLLGTYMIGLGYKYIILNYYTGTPITWKL"
BASE COUNT         2116 a          760 c         1019 g         1847 t
ORIGIN      
        1 aggatttaaa cgaaataatc aattattatc tttgtgcggt ttgaactgtg gtttgtgtcc
       61 gatgcatatt gatggttact gtccaggatg tggtggtgga gcaggaaatc aaagttgtag
      121 gattgctcgt tgtagtttgg aacatgataa tctcacatat tgttatgaat gttcatcata
      181 tccttgtgat aaatacatga aatttgctaa atatgattca tttattacac atcaacatta
      241 tcatcaagat ttaatgaaag ctaaagatgg tggtatagac acatataatc aagagcagct
      301 tgaaaagata gatatttnnn nnnnnnngac gaaaaaagag tttttattgt cttgctgtaa
      361 atcttttaga gttagaagaa cttaaaagtc taattaaaca gatagaaact aattcaaagc
      421 tgtcagctct aacaaaaaaa gagcaagcta aatatgtatt tgatttattt caagatttag
      481 ccaaacaaca tagcttaatc ttgaaattaa ataagaagag taaaaaataa tttacccgta
      541 agattagcaa gagtaactaa tttacgggta tttttattgt acaatcgaag catcatattg
      601 aattgtttgt aataaagctt tacaagtatt aataaattca tccttattca tgcctttaac
      661 aattgcttca ctagctagta actctagctc atcaagtaat ttttcttgat gttcatgaga
      721 attatcaatc gttggttgaa ctttagcacc atgacggcga tcaatacaaa tatagccttc
      781 ttgttttagc agcccataag ctttattaac ggtcatatta tttacttgaa gatcttgagc
      841 taaagtccta acggttggta atgactcgcc gtatttaaat tgaccgttag caataccttt
      901 aacaatttca ttacgtagtt gcaaataaat tggaatttca cttgtatagt ctagattaat
      961 aatcattggc taagcacctc ctgaatttct tgatagatga gtttagtatt ttgaatatca
     1021 tcatcattaa cgatataggt tatatcatca gtatacaaaa ttatatagca aggatgcaaa
     1081 cttgccatgt atactttaca cttaccataa cggtcaaaag tataatttcc cttaccataa
     1141 acaaaagtat cggttccatt agttctaatt cctttaccta aatcatcggc taattctgct
     1201 ttattaactt tttgaagatc aattgaaaca tcgtagaaag gactatcaac aactactgct
     1261 tgatcggtta aagaaagatt gacaagatta ttagttatat ctaaataata cggatatcca
     1321 aataaccata gcaataaagc aataacaaat gtccaaatac caataataaa aattcgataa
     1381 gccggttttg cagtattaaa tgtcatctgt gttccatttg gcgctgaaat aagtgtacgt
     1441 ggatcagctt tattataata cgttggacca aaccaaccga ttttccagca atcatcatta
     1501 ctgatggtat attcacattc attgtaatga gcgagaatct ttgccttttt ctctcgataa
     1561 tggttagctt taatgacaat gataattaca caaagaatca ttattaaggc aactaaaaag
     1621 agtattacaa aaggtagctt ttcaagtaag aaaaattgta aagctaagtt aaaaatacta
     1681 tcaccagtga ttaaaaagaa aaaacaatga tgaaaagaat cacgacgtag taaattcaat
     1741 gttatattta cttcactgtt tttacagaat gttttatttg gtaatttttt aataaagaca
     1801 attccaagta ctaagaccat aaattgtaat accatgtaaa gatacatggc attttcagcg
     1861 tggaaataaa tcatatttcc taatacagat agatcaatta ttaaaacgaa gaagtaacgt
     1921 cttaaattaa aagggtgttt ttccatatag gctgataatg ataaatcaat tttaattttc
     1981 tctatttttt catctggcca attgcgcaat ttttttaaag ctaataattt agcacgtgct
     2041 tttctatacg gaacataata gacggaaacg ttaaaccata tcacaatcat aaaataaagt
     2101 aatagccatt tagttgaaaa aataaataaa ggaataaatg ttataaatga gataataaat
     2161 acccataaat gttctttttt aaactgtttc tcaatttctt taatttcaac agattcataa
     2221 gtccaatcag ggattgtaac taaaaataag cgtccttcat tataacgact ctctcgttta
     2281 gaagaaaagt acattactac attaataaaa actaaacata aaattaaaat ccacatatta
     2341 ctcacctctt atatttgtat tatataacat aatacaaata atacaaaata aaataataaa
     2401 atattataac atattttgct gaaaatgatt cgcaacaaaa agcagaatcg attcgattct
     2461 gccaggttga taattattca gctgtttcta aaccttgttt tttagcagct ttattagaag
     2521 ctgatacaag tggtgcataa actaagagtc caattatggt tataacgatc tgtgagggcg
     2581 taccccgtat atcaccagta gatagataac cagaaagtaa ggggggagtt acccatggga
     2641 taacaatata agtatgtcct actaagccaa ctgcagttgc aaaatatgct attaaagttg
     2701 aaacaaatag aaacaataca gaaaggaaca gtataaatag ggttgaaaac gacaggaagt
     2761 ccaaacataa ttggttcact gatattgaag atacttgaga cctcacccaa acgggcaatc
     2821 actttttctt gaaaagataa atacggtaac aactaagatt aataaaatca ttaagaaaaa
     2881 tgaaactttt gtaagtcagt ataatgatga ttttaaagat gcggttcgtt tcagtgttat
     2941 tgattatggc gcaacagtag gaacagctaa agagatggaa acaaaatttt ctgaaacaat
     3001 tagggttgca agtcaaggtt ataagttaga ggcttatatg catggaccat atttggaagt
     3061 caatccagaa catcgaattt tctttatcga aacgccaagt ccaagattat ctaaagcaaa
     3121 actattaaaa gcctatgaat cacagtatac ggagcacgtg tttgctatta cgaaagcaca
     3181 atcaataaga ccttagcatt agatattgct ttagacgagt atcaggctgc atatttacta
     3241 attattcttt tccaagtaat gacgtggtgt attgctaaaa gtaaagaaat tgatttgacg
     3301 aaatggattt atacagattt ttctcaagcg gtcaagagta aaacggctgt ttaagattat
     3361 gtctagaaag gcttgtcaaa aatatttgac aacattccta atatctttgg tagcattatt
     3421 ttgatggttt atttatacta ttaattatag gaggaaagca gatgttaggc gaaaaattaa
     3481 tgcgattaag aaaaaaacag ggctatagtc aacaagaggt tgcagataag ttatcagtga
     3541 gtagacaaac tatttctaac tgggaatgtg atcaagcctt acccgcagtt gataaagcca
     3601 tggaattggc acagttatat aatataagtt tagatgattt aatggaaaat gagattgaaa
     3661 tagttagtaa taataaaact aaagacctac atcttttaca atatttgatc ggaaaaactt
     3721 gtacgctgga gtgcacacga gatgcctatt tgttagatat atctacaagt gatggaaagg
     3781 ttctaattgt tgatgtcaat gatgattggg ttaaagtgca gtatcatcga acaaaaaaag
     3841 gttcgtttat aaaaaaagaa actgttacca aactaattga tctatcatgt attagcggct
     3901 ttaaggtcga gggggaatta taatgacgac tattattgca attattgctt tagttataag
     3961 tatttgtgca atatatgaga ttgaaaaact aaagaaaaat gatgttaaaa atattgaaaa
     4021 agaaattaag gaaacagatc gaattaagaa tattttagca ggattaaagc agtgtgattg
     4081 cgaattgatt gtaaaagaaa ccatgttttt gattgatgtc ccatatagtg tagaaggtaa
     4141 aatcattgat ttagatgatg attgggtttt aattgttagt atgggaagaa agacgattag
     4201 tagaatgatt aggataacca tgattaaaga tgtaatagaa ttgactaagt agacaactaa
     4261 ggttgtcttt ttaattgtaa actaatttta aataattagt ttacaattta gtgaatgttt
     4321 gctatgctta aaaacgaggt gattcaatga gtgtaaaaca aaatgtaatt gctttacttg
     4381 aagaaaatcg tagtaaggta atatccggac aagagttagc caaccaattg tatgttagtc
     4441 gtgcggcaat ttggaaagcg ataaagacat taaaggaaga gggatataat attgaagcaa
     4501 cgccaaataa aggatatgta ttattagaaa atagtgatgt tttatcaaaa caaggaattg
     4561 catattattt aactgaagaa attgatatct tttcatataa aacaatcgat tcaaccaata
     4621 cacaaatgaa gaaattagca ataaatggtg gtaaaaatca tagtgtaatt gtgtctgaag
     4681 aacaaagcgc cggacgtggt agatttggac gaagttttta ttcaccggct caaaaagggg
     4741 tttatatgag tgttttatta aaaacaggag attcgctaca aaatgcaact atgattacaa
     4801 ttaaaacagc agttgcggtt aggcgagcaa ttgcaaagct atatgatatt gaagtagcaa
     4861 ttaagtgggt taatgatttg tattatcgag ggaagaaggt ttgtggtatt ttaagtgagg
     4921 caattagtga ttttgaaagc ggaatgatcg aagcaattat tattggaatt ggaattaatg
     4981 tatcaactga taattttcca ttagaaatag cttccatagc tacttctcta ggattacaag
     5041 aagctaatcg taatcaattt attgcagaaa tcttaaatca gctatttgca attatcgatg
     5101 aggattttaa attggttttg aatgaatatc gcatggcttc atgtgtattg cataagcaaa
     5161 taacttttaa tcaaaagggg gaacagttta ccggtttagt tcgtgaaatt aatgatttag
     5221 gtaatttggt cgttagtagc aatggtgcag aaatggtatt aaccgcgggt gaagtaagta
     5281 ttataggagg aaaccatgga gcagaataag ttactaacaa caaagaaaat aacctattgt
     5341 gcaattttta cggctctaat tacaatcgga gcatttatac agattccggt acctttcatg
     5401 gattatttta cattacaatt tttctttgtt ttgctagctg gaattttatt aggctcaaaa
     5461 ttaggagcat tggcagtatt actgtatgtt gtaattggat tactaggatt accaatattt
     5521 gcggccggtg gaggtttagc gtatattgtt agacctagtt ttggttattt aattggtttt
     5581 attgcaggag cgtatgttac aggcataata tgtgaaaaaa ctaatgaaat agcagcaaaa
     5641 aaatatgttt tggcagtatt gagtggtctt ttgggcactt atatgattgg acttggctat
     5701 aaatatatca ttttgaatta ctatactgga actccaataa cttggaaact ag
//
LOCUS       DAOMTA010000084         5752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_271_length_5752_cov_2.339477, whole genome shotgun sequence.
ACCESSION   DAOMTA010000084 DAOMTA010000000
VERSION     DAOMTA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_271_length_5752_cov_2.339477"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLK68_03535"
     CDS             complement(<1..828)
                     /locus_tag="PLK68_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRM89516.1"
                     /translation="MKKLLIICLVLLTGCTNANAKVNKIEHQDIFDQVQLSDYQNVMI
                     VAHPDDETIWGGMHLLKDKYLVVCLTNGDNEIRSKEFKEVMKKTQNTGLILNYPDKTN
                     GKRDNWKSSYQKIEDDLNYLLSKQHFNLVVTHNPKGEYGHQHHKMTSAIVDDSFNMIS
                     VDGDTSTNDTALVLANGMAGNKKITEEGADYNAFYEGLSYVFTELSKMIAGDGEGCTC
                     LFEVQVKGATTKAEAKTLAKSIVTSSLTKAAIFGHDANWGRILCAMGYSGADFDPEKV
                     "
     gene            1030..2361
                     /locus_tag="PLK68_03540"
     CDS             1030..2361
                     /locus_tag="PLK68_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89517.1"
                     /translation="MNDELLNGSVFKSLMFFAIPFMLSNLLQVLYGAADLFVVGHFAT
                     TSDVSAVSIGSQTMAMLTNLILGFTTGITVLLGQFLGAKKEKELAATVGSSVVLFTMI
                     AVTISIILFLTNHQIVGLMHTPNDAISATRSYLYICSLGTVFIVGYNAVSAILRGLGD
                     SKTPLLFVAIACVINVIVDFILVDSYQMGAAGAAIATVLAQAGAFIFSLIYLKYKGLG
                     FKFNRQDISFNLPMIAKITKVGLPIGLQSALVGISFLLITVIVNGMGLVASASVGVVE
                     KLIEFLMLPAIALGNAVATMTAQNFGAEQYSRAYKSMRYGIAFCLSISLIVTVVCQFD
                     GSIFTRIFSSDQAVIKNAALYLQTYSFDCLCTSFIFCYNGYLNGGGYTVFTMLHSLLA
                     TFILRIPLTLVISKLSGITLFHMGIASPIASMASIIMCVIYIRYLNKKLLF"
     gene            complement(2660..3118)
                     /locus_tag="PLK68_03545"
     CDS             complement(2660..3118)
                     /locus_tag="PLK68_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM89518.1"
                     /translation="MIAIICAIASGITIVLSRSINGYLAKKIGPYQSTFFNYFTGLLT
                     SSILLFFTLLPAFKNISFTKIDLIMLVGGIIGVFNVLILNIVVSKVTPIKLTLITFIS
                     QLLSGMILDYYIYHVFTLNKLIGCIIVIIGLIIYQSADIKVISNEEINSI"
     gene            complement(3115..3534)
                     /locus_tag="PLK68_03550"
     CDS             complement(3115..3534)
                     /locus_tag="PLK68_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM89519.1"
                     /translation="MYLLLSFLCGLIVSTMNIFNGQLSAHCGVYLATVIIHLIGLITF
                     TLSMFLKKQEISFKHHLPFILYTGGVIGVLTVIFNIIAVNNIGTALLTALGLLGQMII
                     SIILESKGWLGSLKRKLTPLKWLSLIIVTIGIGVMVK"
     gene            3616..4275
                     /locus_tag="PLK68_03555"
     CDS             3616..4275
                     /locus_tag="PLK68_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRM89520.1"
                     /translation="MKNREYYINLLKVEQMMSDDNYQYLELCEFSKAEHLLHQGQTLE
                     YLYILVSGRIKSCRTTANGTTVLSAFSNPITVTGEVEFLNHHEVTNDVYALKNTVCFR
                     ISVAQYEDILLHDLIFMRYLARTLSNRLYHANHNTAISINYPVENRLASYLISSAQQL
                     IIKDNFVQVAEMIGCSYRQLQRVLNDFCQCGYLCKVKRGNYLITDESALKALGQDLYY
                     I"
     gene            complement(4299..4793)
                     /gene="nrdG"
                     /locus_tag="PLK68_03560"
     CDS             complement(4299..4793)
                     /gene="nrdG"
                     /locus_tag="PLK68_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM89521.1"
                     /translation="MNYHNITKDDMLNGEGLRVVLWLSGCTHRCPGCHNPQTWDVTSG
                     IPFDAEAKAEIFTELDKDYISGITFSGGDPLHPSSINEVGQLIKEIKERYPDKTIWLY
                     TGFQFDDIKTIDFIKNIDVIVDGRFEITQLDPQLHWKGSANQRVIAVGPTLAFNTIIL
                     HDDN"
     assembly_gap    4879..4888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4889..>5752)
                     /gene="nrdD"
                     /locus_tag="PLK68_03565"
     CDS             complement(<4889..>5752)
                     /gene="nrdD"
                     /locus_tag="PLK68_03565"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM89522.1"
                     /translation="GCRAFLSPWYRRGGMHPADENDTPVFVGRFNIGAISLHLPLIYA
                     KAKQASKPFFDVLDYYLNLIRKLHLRTYDYLGEMKASVNPLAYCEGGFYGGHLGLHDK
                     IKPILKSATASFGITALNELEQLAHKKSLVEDGSFALKTMEHINQMVEKFKEEDGRLY
                     AIYGTPAENLCGLQVQQFRKKHGVIENVSDRDYVSNSFHCHVSEEISPIEKQDKEKRF
                     WDLCNGGKIQYVKYPIDYNMQAFKTLLKRAMRLGFYEGVNLSLSYCDDCGHEELNMDV
                     CPKCGSKNLTK"
BASE COUNT         1866 a          880 c          990 g         2006 t
ORIGIN      
        1 aaccttttca gggtcaaagt cagcaccaga atatcccatt gcacagagaa ttcttcccca
       61 gttagcgtca tgtccgaata ttgctgcctt agtaagactt gaagtaacaa ttgacttagc
      121 gagtgtcttg gcttctgcct tagttgttgc accctttacc tgaacctcga aaagacatgt
      181 gcagccttcg ccgtcacctg caatcatctt ggaaagctct gtaaatacat agcttaagcc
      241 ttcatagaaa gcattataat cagcaccctc ttctgtaatc ttcttgttgc cagccattcc
      301 attagcaaga acaagagctg tatcattagt tgatgtatct ccatcaactg atatcatgtt
      361 aaatgaatca tcaacaattg cacttgtcat cttgtgatgc tgatggccgt attcaccttt
      421 aggattgtga gtaacaacga gattaaaatg ttgtttcgat aaaagataat ttaaatcgtc
      481 ttcaattttt tgatatgatg atttccaatt atcccgctta ccatttgtct tatcaggata
      541 atttaaaata agtccggtat tctgtgtttt tttcatcacc tctttaaatt ctttagaacg
      601 tatttcgtta tcaccattag ttaggcagac aaccaagtat ttatctttta gtaaatgcat
      661 tccaccccaa atagtttcat catcaggatg agctacgatc ataacatttt gataatcact
      721 taattggact tgatcaaaaa tatcttgatg ttcgatttta tttacttttg cgtttgcgtt
      781 agtacatcct gttaggagga ctaaacagat tattagtagt ttcttcatac ttgaattgta
      841 ccactttatt caattaataa aattaaacct ttcttaatat tgcttaagag aaactaaata
      901 aataaaaagt ttgacagtta agggcaaact tggtaaaatt ttcttgctta ccggagggta
      961 taagaaatta tgctggataa ggtagtgggt caaaccacta ttttatccag ctttttttag
     1021 gaggaaaata tgaatgatga attattaaat ggaagtgttt ttaaaagttt gatgttcttt
     1081 gcaattccct tcatgttatc aaacttatta caagtattat acggagctgc tgatttattt
     1141 gtggtggggc atttcgctac aacaagtgat gtttcagcag tttctatcgg tagtcaaaca
     1201 atggcgatgc taactaacct gattcttggg tttacaacag ggatcacagt attattagga
     1261 caatttttgg gagctaaaaa ggaaaaagaa ttagctgcaa cagtgggttc ttcagttgtg
     1321 ttgtttacta tgattgctgt gactatttca attatattat ttttgactaa tcatcagatt
     1381 gtaggattaa tgcatacacc taacgatgca atcagtgcaa ctagaagtta tttatatatt
     1441 tgtagtttag gaactgtttt tattgtagga tataatgcag ttagtgctat tttaagaggt
     1501 ttaggtgata gtaagacgcc tcttttattt gttgcaattg cgtgtgtaat aaatgttatt
     1561 gtagatttta ttttggtaga tagctatcaa atgggagcag cgggagctgc tattgcaacg
     1621 gtattagcac aagcaggagc ttttattttt tcattgatct atctaaaata taaaggatta
     1681 ggttttaagt ttaatcgcca agatattagt tttaatcttc cgatgattgc gaaaatcaca
     1741 aaagttgggt taccaatcgg cttgcaaagt gctttagttg gtatttcttt tttgttgatt
     1801 acggtaatcg tcaatggtat ggggttagtt gctagtgctt ctgttggagt tgttgaaaaa
     1861 ttgattgaat ttttaatgtt accagcaatt gccttaggta atgctgttgc aacgatgaca
     1921 gctcaaaatt ttggggccga gcagtattca cgagcgtata aatcaatgcg ttatgggatt
     1981 gctttttgtc tttcgatttc attgattgtg acagttgttt gtcaatttga tggaagtatt
     2041 ttcacacgaa ttttttctag tgaccaggca gtaattaaaa atgcggcttt atatttacaa
     2101 acatattctt ttgattgttt atgtacaagt tttatctttt gttataatgg ttatttaaat
     2161 ggtggtggat atactgtttt tacgatgctt catagtttgc tggcaacctt tattttaaga
     2221 attccattaa ctttagtaat tagtaaattg agcggaatta ctttatttca tatggggatt
     2281 gcatcaccta ttgcttctat ggcatcgatt atcatgtgtg tcatttatat tcgttattta
     2341 aataagaagt tattatttta atttttttgc ttatctttac aaaatatact atatgaggtg
     2401 agagaaatga taaaaagaga tagctattta atttatagga gaattaagta caaaggaaaa
     2461 tataagcgaa cggtatgtta ttacttgtag ttatcatttt gaatttttta taaggaaatt
     2521 atgaggattt tatgatctca atatagtaat ggtctgtttt ttacaaatgt gttagatata
     2581 tgaaaaagct aagttagaat atttgaataa aatcatgaaa aagattgtca acgacaatct
     2641 aaaataatta ggaaaattat tagatagaat taatttcttc atttgaaatg acttttatat
     2701 cagcagattg atatataatt aatccaataa tgacgataat acaaccaatc aatttgttaa
     2761 gggtgaagac gtgatagata taatagtcta gtatcattcc actaagtaat tgagagataa
     2821 aagtaattag agttagcttg attggagtaa ctttggaaac gacgatgttt aaaattagaa
     2881 cattaaagac accaattatt cctccaacta acattattag atctatcttt gtaaatgaaa
     2941 tgtttttgaa tgctggtaac agggtaaaaa ataagagaat tgaactagta agcaaaccag
     3001 taaagtaatt aaagaaagtg ctttgataag gaccgatttt ttttgctaaa tatccattta
     3061 tggaacggct taaaacaata gttataccgc tagctattgc acatattatt gcaatcattt
     3121 aaccatcact ccaattccaa ttgtgacaat tattaaactt aaccatttaa gtggtgttag
     3181 ttttcttttt aatgacccta gccagccttt agattctagg atgatagaaa taatcatttg
     3241 acccagtaag cctaatgctg ttaacaaagc tgtaccgata ttattgacag cgataatatt
     3301 aaaaattaca gttagtacac caattactcc gccggtgtat aaaataaagg gtaaatgatg
     3361 tttaaaggaa atttcttgct tttttaaaaa catgctcaag gtaaaagtaa ttaaaccaat
     3421 caaatgaata ataacggttg ctagataaac accacagtgg gcagatagtt gaccattaaa
     3481 aatattcatg gtcgatacga ttaaaccaca taaaaaagat aataataaat acatagttaa
     3541 cacctccaaa tttattataa agaaaagata ttgcttttaa atacgacata tgtcatattt
     3601 aaggtggtga tatatatgaa aaatcgagag tattatatca atttgcttaa agttgagcag
     3661 atgatgagtg atgataatta tcagtatctt gaattatgtg agttttctaa ggctgaacat
     3721 ctattacatc aagggcagac attagaatat ttatatattc ttgtatctgg aagaattaaa
     3781 agctgtcgaa cgactgcgaa tggaacaaca gttttatcag ctttttctaa tccaatcaca
     3841 gttactggag aagttgaatt tctaaatcat catgaagtca ctaatgatgt ttacgcactc
     3901 aaaaatacag tttgttttag aatatcagtg gcgcaatatg aagatatttt attacatgat
     3961 ttaatattta tgcgttattt agcacgaaca ctttcaaatc ggctatatca tgctaatcat
     4021 aatactgcta tttcaattaa ttatccagtt gaaaatcgtt tagcttcata tcttatttcg
     4081 agtgctcaac aattgatcat caaggataat tttgttcaag tagcagaaat gatcgggtgc
     4141 agttatcgtc agttacagcg agtcttaaat gacttttgtc agtgtgggta tctttgtaag
     4201 gttaaacgag gcaattactt gattacggat gaaagtgcat taaaagcatt aggtcaggat
     4261 ttgtattata tataaaaact agggtggcta tggccatcct agttatcgtc atgaagaata
     4321 attgtattaa aagcaagcgt tgggcctact gcaattacac gttgatttgc agaacctttc
     4381 caatgtaatt gaggatctaa ttgggtaatt tcaaagcgcc catcaacgat tacatcaata
     4441 tttttgataa aatcaatcgt tttaatatca tcaaattgga aaccggtata tagccaaatt
     4501 gttttatcag gataacgttc tttaatttct ttaatcaatt gaccaacttc attaattgag
     4561 gaagggtgga gcggatcacc accactgaaa gtaattccag aaatgtaatc tttatcaagt
     4621 tctgtaaaga tttcagcttt agcttcagca tcaaaaggaa taccgctagt gacatcccat
     4681 gtttgagggt tgtgacatcc cggacatcga tgggtacatc ctgaaagcca aagaactact
     4741 cggagccctt caccatttaa catatcatct tttgttatat tatgataatt cattacattg
     4801 atttccgatc tgcaatttct gccatttttg catcatttaa acgcgtatcc cctttaactc
     4861 gtgaatatga taaataacnn nnnnnnnntc tttgttaagt ttttacttcc acattttggg
     4921 caaacatcca tatttaattc ttcatggcca caatcatcgc aataggataa tgatagattt
     4981 acaccttcat aaaagcctaa tctcattgca cgtttaagta aggttttaaa agcttgcatg
     5041 ttgtaatcga ttggatattt gacatattga atcttaccgc cattacataa gtcccaaaaa
     5101 cgtttttctt tatcttgttt ttcaatagga ctaatttctt cagaaacatg acaatggaaa
     5161 gagttgctga catagtcacg gtcactaacg ttttcaatta caccatgttt ttttcggaat
     5221 tgttgcactt gaagaccaca gaggttttct gctggggtcc cataaatagc atataaacga
     5281 ccatcttcct ctttgaattt ttcaaccatt tgattaatat gttccattgt ttttaaagca
     5341 aatgaaccat cttcaactaa tgatttttta tgtgctaact gttctaattc atttaaagca
     5401 gttattccaa aagaagcagt agcagatttt aaaataggtt tgattttatc atgtagacca
     5461 agatgcccac catagaagcc tccttcacaa taagcaagag gattaacaga ggctttcatc
     5521 tcaccaagat aatcatatgt tcgtaaatgt aactttctaa ttaagtttaa ataataatct
     5581 aatacatcaa agaaaggttt acttgcttgt tttgctttag cataaataag aggcaagtgg
     5641 agactgatcg ctccaatatt aaagcgtcct acaaatacgg gagtatcatt ttcatcagca
     5701 ggatgcattc ctcctcgacg ataccatggt gataaaaagg cccggcatcc ca
//
LOCUS       DAOMTA010000085         5669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_278_length_5669_cov_2.526363, whole genome shotgun sequence.
ACCESSION   DAOMTA010000085 DAOMTA010000000
VERSION     DAOMTA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_5669_cov_2.526363"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..518
                     /locus_tag="PLK68_03570"
     CDS             39..518
                     /locus_tag="PLK68_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM89523.1"
                     /translation="MKFNEVMHLSFYTDQMDKMRDFYENKLGLKAKIIMRYGAYLGQK
                     SRGAWAKKAITDPDGIAYIFIELAPGQYLELFNKADNQLEHEKPDVRLGYSHFALMVD
                     DIFEARKELIEAGIEIDIEPNKGQSETWQMWIHDPDGNKFEIMQYTDLSLQHQGNVG"
     gene            complement(552..1328)
                     /locus_tag="PLK68_03575"
     CDS             complement(552..1328)
                     /locus_tag="PLK68_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89524.1"
                     /translation="MIRELIDSIKLTKKEQVISEYLEQEPDCVLQYNAKELAALIKVS
                     APTLIRFVQKLGFKGYSDFQVTYTQEKTMYDQMKNIRIDSKSSIRDVIETLPAIYDHV
                     FKETRKLTRFESFVRTINYMLQAKQIDFYANDNNYSEVQSACLKLNTIGIKAQCFNTL
                     NTAYIDNIDPRDILAFVVSHSGKNQTMVDAAYELRKKRIRVIAITGKIDPTLELVCNE
                     ALYIDSSSHHLPHHIMLYGLSIHYILDILVTSLYYKKYKE"
     gene            1547..2491
                     /locus_tag="PLK68_03580"
     CDS             1547..2491
                     /locus_tag="PLK68_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89525.1"
                     /translation="MELHNQVTRLISAKASKISNYTGRELKEAIFKSEGRVLMGQTYL
                     KNPILFPNCTSTELMFAFGADMVLLNGFDFFHPADCPGMQGFDYQELKDLVGGRPMGI
                     YLGCPKEGLDFNNQENSQLYDLAGMVCTKENLLKAKQWGVSFLILGGNPGSGTSIKDV
                     IKWTKEARAICGDDMLIFAGKWEDGISEKVLGDPLADYDAKNIIKQLIEAGADIIDFP
                     APGSRHGITVEMIRELIEFTHRNGALAMSFLNSNVEAADVDTIRQITLMMKQTGCDVH
                     AIGDGGFGGGTWPENIYQMAITLKGKAYTWAQMATPRR"
     gene            2524..>3747
                     /locus_tag="PLK68_03585"
     CDS             2524..>3747
                     /locus_tag="PLK68_03585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM89526.1"
                     /translation="MSFPDKFLWGGSISAAQCEGAWDEDGKSPVQVDFGDPGTTTNNR
                     YIHYLNADGTRGKMRQFDHLPKGAKYELFDDVRYTNHVGIDFYHRYKEDIALFAELGF
                     TTFNTTISWARIFPHGVEGGVNQAGVEFYRNVFKECRKYGIDPVITLYKYDEPVYLEE
                     TYGDWTNREMIHQFVEFAKVCFIEYKDLVNKWLTFNEINILLHFNVKEGKQFAFEELH
                     NQMVAAAEAVKAAHEIDEEIKVGCMIAGFCCYSYTCDPKDVLGSYKLFQEKFAYCADT
                     MVRGYYPAYAKRIWKDNNVSLEISEEDKKILMEGKSDFLAYSYYMSNVFTTHDVEGNL
                     ATAGGQGSLANPYLEASDWGWQIDPTGYEYFLHVLNDRYQVPLFDVENGLGAHDQVED
                     DGSIHDDYRINYHRSH"
     assembly_gap    3748..3757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3758..3974
                     /locus_tag="PLK68_03590"
     CDS             <3758..3974
                     /locus_tag="PLK68_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89527.1"
                     /translation="MKAREEGVNISGYTSWAPIDLVSFTTGQMDKRYGFIYVDMNDDG
                     VGDLHRIKKDSFYWYQKAIKSDGKDLD"
     gene            4696..>5669
                     /locus_tag="PLK68_03595"
     CDS             4696..>5669
                     /locus_tag="PLK68_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM89528.1"
                     /translation="MLKVAYIGFGNSVCRYHLPYVEKRKDFIDVKYIYRLETEINEEK
                     ESWYPGIKFTSSINEIMDDPDVNLVVVNTPDRYHVEYTMQALDHGKHVLCEKPFAMTA
                     KEAKDVFDYAKEKGLVAMANQNRRYDADMRTVRKVIESGVLGDIVEIESHYDYFRPSI
                     ANNKGMGILYGLAVHPIDQIIGEFGKPNRVVYDCRSVDNPGVSDDYYDFDFFYDGFKA
                     IVKTSYYVKLDYPRFIVHGKKGSFLMPALGHNSSEKPKPGAIHVSFDPLPEDKWGTLS
                     YIDDNGNDITKKVPTEIGDYGIIYDNLNDVIVNGAHKLVADEEVIEVL"
BASE COUNT         1932 a          688 c         1186 g         1853 t
ORIGIN      
        1 atttaaacag acctttttat aaaaatgcga ggttaaaaat gaaatttaat gaagtgatgc
       61 atctttcatt ttatactgat cagatggata aaatgagaga tttttatgaa aataaattag
      121 gattaaaagc taaaataatt atgcgttatg gggcttatct tggacaaaaa agccgtggag
      181 cttgggctaa aaaagcaatt actgatccag atgggattgc ttatattttt attgagttag
      241 ccccgggaca atatttggaa ttatttaata aagcagataa ccagctagaa catgaaaagc
      301 cagatgttag actagggtat tcacactttg ctttaatggt tgatgatatt tttgaagcac
      361 gaaaagagct gattgaagct ggtatcgaaa ttgatattga gcctaataaa ggtcaatcag
      421 aaacttggca aatgtggatt catgatccgg atggaaataa atttgaaatt atgcaatata
      481 ctgatttatc tttgcagcat caaggaaatg ttggttaaat aagaccctac gggagcgtaa
      541 ggtcttgttt attattcttt gtattttttg taataaagtg aggtaacgag aatatctaaa
      601 atataatgta tcgataatcc atatagcata atatgatggg gcagatgatg tgaggatgaa
      661 tcaatataca aggcttcatt gcatacaagt tctaaagttg ggtcgatttt tcctgtaatt
      721 gcgatgacac ggattcgttt ttttcggagt tcatatgcgg catcgaccat tgtttgattt
      781 tttcccgagt gactaactac gaaagctaag atgtctcgag gatcgatgtt atcaatatag
      841 gcagtattca aagtgttaaa gcattgtgct ttgataccaa tggtatttag ttttaaacag
      901 gcagattgaa cttctgaata attattgtca ttggcataaa aatcaatttg tttagcttgc
      961 agcatataat ttatcgttcg aacaaacgat tcaaaacgag ttagttttcg agtttcttta
     1021 aaaacatggt catatattgc aggtagggtt tcaattacat cacgaataga actttttgaa
     1081 tcaatccgta tatttttcat ttgatcatac attgtttttt cttgggtgta agtcacttga
     1141 aaatcactat agcctttaaa tcctaatttt tgaacaaagc gaataagggt gggggcacta
     1201 actttgatta aagcagctaa ttctttagcg ttatattgaa gcacgcaatc aggctcttgt
     1261 tctaaatatt cggaaataac ttgttctttt tttgtcagtt taatgctatc gattaattca
     1321 cgaatcattt tatgcactcc tttttaggga ttatttatac ttatatttta acatattaat
     1381 agacatagag aaaacgtttt ctcactacta atgatatttt tgaaattaaa tttcataatt
     1441 atttatatct ttgaaattta attacaaaat atctatatat gattttttta gactactttt
     1501 taaattaaga tttagttgta agaaaataga tataaggaga aaaaagatgg aactacataa
     1561 tcaagtaact agattgatta gtgcaaaagc tagtaaaatt tctaattata ctggtagaga
     1621 attaaaagaa gcaattttta aatctgaagg tagggtgctg atgggacaaa cgtatttaaa
     1681 aaatccaatc ttatttccta attgtaccag taccgaatta atgtttgctt ttggtgcgga
     1741 tatggtttta ttgaatggtt ttgatttttt tcatccagct gattgtcctg gcatgcaagg
     1801 atttgattac caagaattga aggatttagt aggtggtaga ccaatgggaa tttatttggg
     1861 gtgtcctaaa gaagggcttg attttaataa ccaagagaat tctcagttat acgatctagc
     1921 aggtatggta tgtacaaaag aaaatctttt aaaggcaaaa caatggggtg tatcatttct
     1981 cattttaggt ggtaatccgg gaagtggtac atcaattaaa gatgtgatta agtggacaaa
     2041 agaggctaga gcgatttgtg gtgatgatat gctgattttt gctggaaaat gggaggatgg
     2101 cattagtgaa aaagtattag gtgatccact tgctgattat gatgctaaaa atattattaa
     2161 gcagttgatt gaggctgggg ctgatattat tgattttcct gctccgggct cacgccacgg
     2221 tattacagtt gagatgattc gtgaattaat tgaatttacg catcgaaatg gggctcttgc
     2281 tatgagcttt ttaaatagta atgttgaagc agcagatgtt gatacaattc gtcaaataac
     2341 actgatgatg aaacaaacag gatgtgatgt tcatgctatt ggtgatggtg gttttggtgg
     2401 tggaacctgg ccggaaaata tttaccaaat ggcaatcact ttaaaaggaa aggcgtatac
     2461 ttgggcgcaa atggcaacac caagaagata aaaataaaag aaggcttaag gaggataata
     2521 aagatgagtt ttccagataa atttttatgg ggcggttcga ttagtgcggc tcaatgtgaa
     2581 ggagcatggg atgaagatgg taagagtccg gttcaagtag atttcggtga tcccggaaca
     2641 acaactaata atcgttatat tcattattta aatgctgatg gtacaagagg aaaaatgcga
     2701 caatttgatc atttaccaaa gggggcaaaa tatgaattat ttgatgatgt tagatataca
     2761 aatcatgtag ggatagattt ttatcatcga tataaggaag atattgcctt atttgctgaa
     2821 cttggtttta caacttttaa cacgacgatt tcttgggcta gaattttccc gcatggtgtt
     2881 gaaggtggag taaatcaagc aggagttgaa ttttatcgta atgtttttaa agaatgtagg
     2941 aagtatggaa ttgatccagt tataactttg tataaatatg atgaaccagt ctatttggaa
     3001 gaaacctatg gtgactggac aaatagagag atgattcatc agtttgttga atttgctaaa
     3061 gtatgtttta ttgaatataa agatttagtc aataagtggt taacctttaa tgaaattaac
     3121 attttgcttc actttaatgt taaagaagga aaacagtttg cttttgaaga attacataat
     3181 caaatggtag cggcggcaga agcggtaaaa gctgctcatg aaatagatga ggaaatcaaa
     3241 gttggttgta tgattgcagg gttctgctgt tattcatata cgtgtgatcc aaaagatgtt
     3301 ttaggttcat ataagttgtt tcaagaaaaa tttgcatatt gtgcagatac gatggtaagg
     3361 ggatattatc cagcttatgc aaaacgtatc tggaaagata ataatgtatc tttagaaatt
     3421 agtgaagaag ataagaagat tctaatggaa ggtaaatctg actttctagc atattcatac
     3481 tatatgtcaa atgtatttac tacgcatgat gtagagggaa acttagcaac agcaggtggt
     3541 cagggtagtt tagccaatcc atatttagaa gcatctgatt ggggctggca aattgatcct
     3601 acaggttatg aatatttttt acatgtactg aatgatcgat atcaagtacc gttatttgat
     3661 gtggaaaatg gtttgggggc acatgatcaa gtggaggatg atggctctat tcatgatgat
     3721 tatcgaatta attatcatcg cagtcatnnn nnnnnnnaat gaaagctcgt gaagagggcg
     3781 ttaatatttc tggttataca tcatgggcac caattgattt agtatcattt acaacaggac
     3841 aaatggataa gcgatatggg tttatttatg tagatatgaa tgatgatgga gttggtgatt
     3901 tacatcggat aaaaaaggat tcattttatt ggtatcaaaa agcgattaaa tcggatggaa
     3961 aagatctaga ttaagatgtc tgttagagac atctttttta agtatgacga cttgtcatac
     4021 atctagggtg aaagtccttt gtgaatgtag ttgtcgacga atcacatagt gcatcggaaa
     4081 tgtatttgag ccgaagggca tatacacttg gatctaggca ttagaaagcg aaaaggagaa
     4141 gatcacacaa attattagag gaagtcttat ctaagaaaaa tatgatactt gtctataaaa
     4201 gcacaagtag tgtggataat gtagccaatg atgaaataaa agacaaattg aaacaaggtc
     4261 ttacaaacca caacctgtta aaagtattga aattccaaag cctagtcatg gtgttagaaa
     4321 acttggggta aattatggta gagcaagaca aatatcatat gctatcaatt cgtgataact
     4381 agaacatgtg ttgtgcgtgc tgtagcaaaa gaaaggctag cacacaatgg actagttagt
     4441 tgtttagatt attatttatt gagatataaa ttgaaattta attaaactgc cgtatatgga
     4501 accgtatgga cagtggtgta agagagggaa aatatttaaa attttccgct acttgattaa
     4561 tttaatttag tagtatgaaa ttaaattaca gtactgtaaa taaatgtatg aaattcaatt
     4621 gcaagagctg gaaaatcact gttttatagg tttttttcat cataaaataa acctataaaa
     4681 taggaggaaa gaaagatgtt aaaagttgct tatattggat ttggtaacag cgtttgtcgt
     4741 tatcatttac catatgttga aaaaagaaaa gacttcattg atgtaaagta tatttatcgt
     4801 ttagaaacag aaattaatga agaaaaagag agctggtatc ctgggattaa gtttactagt
     4861 tcaattaatg agattatgga tgatcctgat gttaacttgg tagttgttaa taccccagat
     4921 cgttatcatg tggagtatac aatgcaggca ttggatcatg gtaaacatgt tttatgtgaa
     4981 aaaccatttg caatgaccgc taaagaagct aaagatgtat tcgattatgc taaagaaaaa
     5041 ggtctagtag caatggctaa ccaaaatcgt cgttatgatg ctgatatgcg cacagttcgt
     5101 aaagttattg aatcaggtgt acttggagac attgtggaaa ttgagtcaca ttatgattat
     5161 tttagaccat caatagcaaa taataaaggt atgggaattt tatatgggtt agcagttcat
     5221 ccaattgatc aaatcattgg tgaattcggt aaacctaatc gagttgtata tgactgtcgt
     5281 agtgttgata atcctggagt cagtgatgat tattatgatt ttgatttctt ttatgatgga
     5341 tttaaagcaa ttgttaaaac tagttattat gtaaaacttg attatccacg ctttatcgtt
     5401 catggcaaaa aaggaagttt cttaatgccg gcacttggtc ataattcatc agaaaaacct
     5461 aaaccaggag cgatccatgt atcatttgat ccattaccag aagataagtg gggaacattg
     5521 tcttacattg atgataatgg caatgatatt acaaaaaaag tacctacaga aattggggat
     5581 tatggaatta tttatgacaa tttaaatgat gttattgtaa atggggctca taaattagtt
     5641 gccgatgagg aagtaataga agttttaaa
//
LOCUS       DAOMTA010000086         5642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_282_length_5642_cov_2.763200, whole genome shotgun sequence.
ACCESSION   DAOMTA010000086 DAOMTA010000000
VERSION     DAOMTA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_282_length_5642_cov_2.763200"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLK68_03600"
     CDS             complement(<1..483)
                     /locus_tag="PLK68_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM89529.1"
                     /translation="MLKLFKKKEKQNKVLSSMANGKSVAIEDVPDEVFSTKMMGDGIA
                     VIPDDGKIYAPCNGKISMVMDNTKHALGIETEDGMELLIHVGLDTVNLMGEGFATHVS
                     AGDSVETGELLISYDKTDFVTKGINDITMLVIVENGGHEITKYNINENVHIIESPLIE
                     Y"
     gene            complement(485..1816)
                     /locus_tag="PLK68_03605"
     CDS             complement(485..1816)
                     /locus_tag="PLK68_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM89530.1"
                     /translation="MEKKYSVVIAGGGSTFTPGICLLLLDHMDRFPIRKIKFYDNFAE
                     RQETIAKACEIYLKENAPEVEFAYTTDPEEAFTDVDFVMCHIRVGLYAMREKDEKIPM
                     KYNVVGQETCGPGGIAYGMRSIGGVIEILDYMEKYSPNAWMLNYSNPAAIVAEATRRL
                     RPDSKILNICDMPIDLEEKMANMVGLKSRKEMSVGYYGLNHFGWWHKIEDKDGNDLMP
                     EIKKHMAANGFADALSGTNQHVEQSWIETFAKAKDVYALDPETIPNTYLKYYLYPDYV
                     VEHTNPEHTRANEVMEHREKNIFTACRKIIEKGTAVDGGFEPDAHAEYIVDLACAIAK
                     NTKEKMLLIVPNEGAVENFDRTAMVEIPCIVGSNGYERICQGSIPQFQKGLMEQQVSV
                     EKLVVDAWITGSYQKLWQAITLSKTVPSARVAKLILDDLIEANKDFWPELK"
     gene            complement(1836..2327)
                     /locus_tag="PLK68_03610"
     CDS             complement(1836..2327)
                     /locus_tag="PLK68_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89531.1"
                     /translation="MAKRINEKKLKRLDRYYMIAKVFLMVTPFIAYLYLSLLAMMRSI
                     TLPEVLSSEPSVAVIFLIAMINPYIAYLLNIAQRKLKEGDIKFACINFVLLLLAQALT
                     LNSLYFMIIAYLFYVTVKTYDIKVFKTLKEFTMKYTFQYGGGSFIVVAFSTVCLFATL
                     RLM"
     gene            complement(2353..>3100)
                     /locus_tag="PLK68_03615"
     CDS             complement(2353..>3100)
                     /locus_tag="PLK68_03615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HRM89532.1"
                     /translation="PWAWPTLTGLSKVFGCPGIALAFYATAKDKKKKQIAGLLIPITL
                     TAIVCGITEPIEFTFLFIAPVLFVVHAVLAATLATVANALGVVGVLSGGLIEMSALNF
                     IPLMASHWQTYLTLLVVGLVFTGIYFIVFRFLILKFDFKTPGREDDEEEIKFHTKADF
                     KEKQAGGKVEKQSLAAAILEGLGGKDNIVDVTNCATRLRVNVKDEKLVQSDSYFKSIG
                     THGLKATKTNIQVIVGLKVPSVREDFEALL"
     assembly_gap    3101..3118
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3119..3930)
                     /locus_tag="PLK68_03620"
                     /pseudo
     CDS             complement(3119..3930)
                     /locus_tag="PLK68_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301236.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            4180..>4603
                     /locus_tag="PLK68_03625"
     CDS             4180..>4603
                     /locus_tag="PLK68_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HRM89533.1"
                     /translation="MINLCGFLCMYDYMNDLTNQVQNFNKMLTLLKYRGPDDFNIASS
                     EHVLLGHCRLSIIDLNGGKQPFKYTYNDIEYTIVYNGEIYNMNTIKEQLIDEGYHFTS
                     QSDTEVVVASFIAYGPRCLNLFDGIFSFVISYQNKLFVA"
     assembly_gap    4604..4613
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4614..>5642
                     /locus_tag="PLK68_03630"
     CDS             <4614..>5642
                     /locus_tag="PLK68_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRM89534.1"
                     /translation="RDQLGVKPLYYYQKDDLFIFSSEIKCILMYLGRCVVDETGLKEL
                     LGLGPSVSPGKTIYKDIYSLRPAHYMNVFNGYKEINRYWALERRNHNRSYEETVHDIR
                     CLVNHSIRQQLLSDVPVSCMLSGGLDSSIITGVTSQYISKLSTYSVDYQDQDKYFQPY
                     EYQTTRDDHYIDEVKTLYNTKHKTVTLSQKQLVMSLKESLIARDAPGMADIDSSFLLF
                     SKEISHNHKVVLSGECADEIFGGYPWFYRKELYSIDGFPWMRDLDKRMELFSDEIQAL
                     NIKNYVMDKYHQTLNEIDYHDHTYEDANKRKMIYLNMEWFMQTLLTRSDSQTMRSSIE
                     LRVPFANKD"
BASE COUNT         1808 a         1068 c          834 g         1904 t
ORIGIN      
        1 gtattcgatt aaaggagatt ctattatatg aacattttca ttaatattat attttgtgat
       61 ttcatgacca ccattttcaa caataactaa cattgttata tcattgattc ctttagtcac
      121 aaaatctgtt ttatcataag aaattaataa ttcaccagtt tctacactgt caccggcaga
      181 tacatgagtg gcaaaaccct cacccattaa attaacagtg tctaagccaa catgaattaa
      241 taactccata ccatcttcgg tttcaatccc caaagcatgt ttcgtattat ccatcaccat
      301 tgagattttc ccattacatg gcgcataaat tttaccatca tctggaatta cagcaattcc
      361 atcacccatc atttttgttg aaaaaacttc atctggtaca tcttcaatgg ctacactttt
      421 tccgttagcc attgaagata gtactttatt ttgtttttct ttctttttaa atagttttaa
      481 catcttattt caattcaggc cagaaatctt tattcgcttc aattaaatca tctaagatta
      541 atttcgctac tcttgcactt ggtactgttt ttgataatgt gattgcttgc cataattttt
      601 gatagcttcc tgttatccat gcatctacta ctaatttctc tactgatact tgttgttcca
      661 ttaatccctt ttggaattgt ggaattgatc cctgacagat tctttcatat ccgttgcttc
      721 ctacaataca tggaatctct accattgccg tacggtcaaa gttttctact gctccttcat
      781 tcggtacgat caataacatt ttttctttag tgtttttagc aattgcacaa gctaagtcaa
      841 caatatattc agcatgcgca tctggttcaa accctccgtc tacagctgtt cctttttcaa
      901 tgatctttct acaagctgta aagatattct tttctcgatg ctccattact tcatttgccc
      961 gtgtatgttc cggattagta tgttctacta cataatcagg atataaataa tattttaaat
     1021 atgtgtttgg gattgtttct ggatctaatg cataaacatc ttttgctttt gcgaatgttt
     1081 caatccagct ttgttccacg tgctgattcg ttcctgacag tgcatctgca aaaccattag
     1141 ctgccatatg cttcttgatt tccggcatta agtcattacc gtctttatct tcaattttat
     1201 gccaccatcc aaagtggttt aatccataat atccaacaga catctcttta cgtgacttta
     1261 accctaccat gttagccatc ttttcttcaa gatcgattgg catgtcacag atatttaaga
     1321 tcttagaatc aggacgtaat cttcttgttg cttcagctac gatggcagct ggattagaat
     1381 aatttaacat ccaggcattt ggagaatatt tttccatgta atccagaatt tcaataaccc
     1441 cgccaataga acgcatgcca taagcaattc caccaggacc acaagtttct tgaccaacaa
     1501 cattatattt cattggaatc ttttcatctt tttcacgcat tgcatataat ccaacacgaa
     1561 tatgacacat aacgaagtca acatcagtaa aagcttcttc tggatcagta gtataagcga
     1621 actcaacttc tggtgcattt tcttttaaat aaatttcaca ggctttagcg atggtttctt
     1681 gacgttccgc aaaattatca taaaatttaa tttttctaat tggaaaacga tccatatgat
     1741 ctaacaataa taaacaaatc ccaggtgtga aagtacttcc accaccagca ataacaactg
     1801 aatatttctt ttccattttc tttttctcct ctgttttaca ttaatctaag cgtcgcaaat
     1861 aaacaaacgg ttgaaaaggc tactacaatg aagcttcctc caccatactg gaaagtgtac
     1921 ttcattgtaa attccttaag ggtcttgaat actttaatat catatgtttt aactgttaca
     1981 tagaacaagt aagcaataat cataaaatat aaactattta atgttaaggc ctgagctaaa
     2041 agcaataata cgaagttaat acaagcaaat ttaatgtcac cttcttttaa ttttctttgt
     2101 gcaatattta gtaaataagc aatatatgga ttaatcattg caatcaagaa aattactgct
     2161 acacttggct cactgctcag cacttctggc aaagtaattg aacgcatcat cgctaacaac
     2221 gataaatata gataagcaat aaacggtgta accattaaga acactttagc gatcatataa
     2281 tatcgatcta atctttttaa cttcttttca ttaattcttt ttgccatctt caagaccatc
     2341 cttttcattt atttatagta atgcttcaaa atcttctctt acacttggta cttttaatcc
     2401 aacaattact tgaatatttg tcttagtagc ttttaaacca tgagtaccaa ttgatttaaa
     2461 atagctatca gattgaacta atttttcatc ttttacatta actcttaaac gagttgcaca
     2521 gttagtaaca tcaacaatgt tatctttacc acctaatcct tcaaggatag cagcagctaa
     2581 actttgtttt tctacctttc caccagcttg tttttcttta aaatcagctt tagtgtggaa
     2641 tttaatttct tcttcatcat cttcacgacc aggtgtcttg aagtcaaatt ttaaaatcaa
     2701 gaatctaaat acaatgaaat aaatacctgt aaagactaaa cctactacta ataatgttaa
     2761 atatgtttgc cagtgtgaag ccattaatgg tataaagtta agggctgaca tttcgatcaa
     2821 accacctgat aataccccaa caacacctag cgcatttgct actgtagcta aagtagcagc
     2881 taatactgca tgtactacaa acaatactgg tgcaataaat aagaatgtaa attcaattgg
     2941 ttctgtaatt ccacaaacaa tagctgttaa tgtgattgga attaatagac cagcaatttg
     3001 ttttttcttc ttatctttcg ctgtcgcata aaatgctagt gcgatacctg gacatccaaa
     3061 tactttagat aaacctgtaa gtgttggcca tgcccaaggt nnnnnnnnnn nnnnnnnngc
     3121 taatgatttt aaagaatcac ttgaagccgc taaatctggt aaagctgttg cccaagctga
     3181 ataaactcca ccgttgataa ttactgaatc gtagaagaat gggaaataca ttaaatgatg
     3241 taatccaaat ggaattaaga tacgttccat gaagataaag atccataccc cgattgatcc
     3301 agcaccagca acaaatcctt ggaatgcacg cataccatct tgaacgattg gccataatca
     3361 tgcagataaa gaagcaactg gtaataaagc aaagaaacca acaattacta cgaatgatga
     3421 accactgaat gttcctaacc attcaggtaa ttcagtatca aagaatttat tgtgtaaata
     3481 tacaacgata catgaaatca ttaacgctcc gatcatcccc atatctagag ttttaatact
     3541 agcaatcatc gtaagaccac ttgtacctcc agtttctgct gcgaagtcaa ctccgaaagt
     3601 tcctccccat tggcttaaga tcgtacttaa gaaatattga aatgttagat aaagtactaa
     3661 cgcttccata caacatcttg cttgttgttt tttcgcaaga ccgattggta atccaaccac
     3721 gaagattaat ggtaactgat tgaatactgt ccatccccct tgaagaatga cattccatac
     3781 ttgataccac aatccattag ggtcagctag accacccata attgcttcag ttgtaaacaa
     3841 tgttccaatc ccgatagttg ttcctgcaaa agcaaataac agtaccggcg tgaacattgc
     3901 cccaccaaaa cgttgaattt tttgcaacat attttgtcct cccttttctc ttgacacata
     3961 catcgtatca atttgaaagc cgttacacaa tactttgacc aaagttagag acaaggtgat
     4021 attttctatt atttatcaca ttcaccgtga tttttcacgc tagtgcaaaa cccttgatat
     4081 acctaatttt tcgcattttt atggagaatc aaaaaaagaa aacacggtta aaattatctt
     4141 ttgacataat aaaaagtatc attcatataa tgatacgggg tgattaattt gtgtggattt
     4201 ttatgtatgt atgactatat gaacgattta actaatcaag ttcaaaactt taataagatg
     4261 cttacattat taaaatatcg tggacctgat gattttaata tcgccagtag tgaacatgtt
     4321 ttattaggcc attgtcggtt atcaattatt gacttaaatg gcggtaaaca accatttaaa
     4381 tatacttata atgatattga gtatacgatt gtatataatg gtgagattta caatatgaat
     4441 acgatcaaag agcaattaat tgatgaaggg taccatttta ctagtcaaag tgataccgag
     4501 gtagttgttg ctagttttat tgcttacggt ccacgttgtc ttaatttatt tgatggaatt
     4561 ttctcttttg taatttcata tcaaaataaa ctttttgtgg ctcnnnnnnn nnncgtgatc
     4621 aacttggtgt caaaccgctt tattattatc aaaaagatga tttatttatt tttagtagtg
     4681 aaataaaatg tattttaatg tatttagggc gttgtgtcgt tgatgaaaca ggacttaaag
     4741 aattattagg actagggcca agtgtttcgc ctggtaaaac tatttataaa gatatctata
     4801 gtcttcgacc agcacattat atgaatgtct ttaacggtta caaagaaatc aatcgttatt
     4861 gggcacttga gcgacgtaat cataatcgga gttacgaaga aacagttcat gatatccgct
     4921 gtctcgtaaa tcatagtatt cgccaacagt tgttgagcga tgtccctgta tcatgcatgc
     4981 tatctggcgg ccttgattct tcgatcatca ctggtgttac tagccaatat atttccaaat
     5041 tatcaactta tagtgtcgac tatcaagatc aagacaaata cttccagcct tatgagtatc
     5101 aaacaacacg tgacgaccac tatatcgatg aagtaaaaac actgtataac accaaacata
     5161 aaacagtaac tttatcccaa aaacaattag tgatgtcgtt aaaagaatca ttgattgcgc
     5221 gtgatgctcc tgggatggca gatattgatt caagtttctt attattttca aaagaaattt
     5281 ctcataatca taaagttgta ctttccggag aatgtgcgga tgaaattttt ggtggctacc
     5341 catggtttta tcgaaaagaa ctttacagta ttgacggctt tccatggatg cgtgatttag
     5401 ataaacgaat ggaactattc tctgatgaaa ttcaagcact gaatatcaaa aactatgtca
     5461 tggataagta tcatcaaaca ttaaacgaaa ttgattatca tgatcatact tatgaagatg
     5521 ctaataaacg taaaatgatc tatttaaata tggaatggtt tatgcagact ttattgactc
     5581 gttctgatag tcaaacgatg cgtagttcta ttgaattacg agttcctttc gctaataaag
     5641 at
//
LOCUS       DAOMTA010000087         5612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_286_length_5612_cov_2.059924, whole genome shotgun sequence.
ACCESSION   DAOMTA010000087 DAOMTA010000000
VERSION     DAOMTA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_5612_cov_2.059924"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLK68_03635"
     CDS             <1..292
                     /locus_tag="PLK68_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM89535.1"
                     /translation="HRENGESIEACARRELYEETGAVTYTLERLCDYGVVEKTGSKES
                     FGTIFYAEIEKMADLPDSEIEKIFLSKELPECWTYPEIQPYMIDEYKRRRGS"
     gene            293..733
                     /locus_tag="PLK68_03640"
     CDS             293..733
                     /locus_tag="PLK68_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HRM89536.1"
                     /translation="MKKIFIVMMIAGILTGCSDSSTKSITVCKGSFDYGDIENTYEAK
                     GDKVIRIVNVTINDYRDTDYDDETLVSTLESQLNVYKEIDGLEIKLTAKDQIVRQEFI
                     IDIENGDLKKLNEVGLIDLNGDGTVSFEKLTKNAKENKLTCKEK"
     gene            773..1384
                     /locus_tag="PLK68_03645"
     CDS             773..1384
                     /locus_tag="PLK68_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89537.1"
                     /translation="MARKPLKLTKNALMLLGIIALLLITFLVLKFGGTKSEQPEKKVT
                     ETLSGLVVENQVLKVQLLDFVSNKDFDDKYQEVSMDIKADEEVLNYKISNRQVFNKVM
                     QLLPPGEGSPLLNNSSEVPTHEAYILVLTGDIVEYKDSEGKSSYQIANARLDYYKQSL
                     LLENDYDSVYIASIDGKKEKMVKITAYKEALSSPSEYMTMLQW"
     gene            complement(1426..1761)
                     /locus_tag="PLK68_03650"
     CDS             complement(1426..1761)
                     /locus_tag="PLK68_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89538.1"
                     /translation="MQALFLVLHKVEKLDDLLAALQKSGINGGTIIESKGMLNTLKSN
                     DNFIIESLRIFLEDPRETSKTLFFILKNEDVEKARTVIDKTLGGIDKPNTGIMFGIDL
                     TFVAGLNIE"
     gene            1876..3057
                     /locus_tag="PLK68_03655"
     CDS             1876..3057
                     /locus_tag="PLK68_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM89539.1"
                     /translation="MNFEFSNRISGVKASAIREILKMMNDPEIISFGGGNPASETFPV
                     EQLKRISDDIFNTCPNSILEYGITEGDGSFKKAAIEFLSRHETVVKDYDGVMVTSGSQ
                     QIMDFLSKCLCNEGDLVVCENPSFLGALNAFKSNGAKLVGIDMEDDGINLEQLEAVLS
                     GVQKPKFIYLIPNFQNPTGITTSLEKRQAIYTLAKKYQVLILEDNPYGDLRFRNDAIP
                     SIKSLDDEGLVVYAASFSKIIAPGMRVAVMIGHQDLLAKCTVAKQTNDVHTNAWAQSV
                     MARFLNETDMEVHLKKLQAVYEQKCNLMLEEMKKQFSPDCKWTIPDGGMFIWVTLPER
                     IDMPEFVKRAVEHKVAVVPGNAFYDDDNKPCQSFRMNFSTPTNEQIIKGVAILGALMH
                     D"
     gene            3050..3655
                     /locus_tag="PLK68_03660"
     CDS             3050..3655
                     /locus_tag="PLK68_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM89540.1"
                     /translation="MIKNIVFDMGNVILRWDPHYIASKLSNDSLEQDVIERELFASKQ
                     WQKLDQGLLTVEEALKELKTDNPQLIRHALLHWYDYFEPFAEMVALVKELKEQGYHIY
                     LLSNCSLQFDDYYQNIEAFKYFDDFYISARYQLLKPDLEIFKHFLSKFNLKAEECIFI
                     DDIKANVEGAKIAKMHGYQHNGDIKKLRLYLKKQHDLPINY"
     gene            3761..5263
                     /locus_tag="PLK68_03665"
     CDS             3761..5263
                     /locus_tag="PLK68_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM89541.1"
                     /translation="MDKQEENMSNTNDTIESKQKSEMTKKVRSGEKTGVQKKIIIIGI
                     LVSLLVIIYLIGSFYFNKKFLSGTYINDINVSGLTLTEANEKLAEQIDARSLKLIFND
                     EATETVSGSECGVRYNSNNNVKNILKKQNSFGWIGAYFNKSKNVVNDLAEVDETVLRA
                     KVASLGHLQEEVQVAPVDAKVEYLNNEFTITNEVNGSTIDQEKLISEIKLAFSEGKES
                     LNLTEKKCYVEPAVKANDAKLQNLLDAARKYASAAITYKTRSGDVVLDGSTLVTWLSI
                     DESGNYYRDDAVFKEKVTDFVGSLAKINSVGGSRTFVGANNRTITVSGGNYGLRLQNS
                     KEISGLLEDIYANKVGVRTPVTTGREASSDNGGLGDTFVEIDLSGQHMWYHKNGQIVL
                     ESDIVSGTYNIADRRTPAGAYYLYNKERNRVLRGTKLPDGTWPYETPVSYWMPFNKGI
                     GLHDSSSWRSKWGGTIYMNNGSHGCINLPTGIASQLYNSIEVNCPVVCYY"
     gene            5430..>5612
                     /locus_tag="PLK68_03670"
     CDS             5430..>5612
                     /locus_tag="PLK68_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM89542.1"
                     /translation="MENYVIDFATKILNIDSPTGYCKEVIEFVKNEVEQLGYRTGMNN
                     KGNLYIYVDGKNDKTIG"
BASE COUNT         2030 a          651 c         1114 g         1817 t
ORIGIN      
        1 acatcgtgaa aatggggaaa gcattgaagc atgtgcaaga cgtgaattgt atgaagaaac
       61 gggagctgtt acttatacgc ttgaacgtct ttgcgactac ggagttgttg agaaaactgg
      121 atcaaaagag tcttttggaa caatatttta tgccgaaatt gaaaaaatgg cggatttacc
      181 tgattctgag atagaaaaaa tatttttatc gaaggaactg cccgaatgct ggacatatcc
      241 ggaaattcag ccttacatga tcgatgaata taaaagacgg agaggatcat aaatgaaaaa
      301 aatatttata gtaatgatga tagcaggaat tttaactgga tgtagtgatt catctactaa
      361 gagtataaca gtttgtaaag gaagttttga ttatggcgat attgaaaata cttatgaagc
      421 taaaggcgat aaggttatca ggatcgtgaa tgtgacgatt aatgattata gagatacgga
      481 ttatgatgat gagaccttag ttagtacact tgaatcacaa ttaaatgtat ataaagaaat
      541 agatgggttg gaaataaagc ttactgctaa agatcaaatt gttcgtcagg aatttataat
      601 tgatatagaa aatggtgatt taaaaaaact taatgaggtt ggattaatag atttaaatgg
      661 tgatggaacg gtttcttttg aaaaattaac aaaaaatgct aaagagaata aattaacttg
      721 taaagaaaaa taagcaggag atatgatata ataagtaaaa ggaggtgtga ttatggcacg
      781 aaaacctctg aaattaacga aaaatgcgtt aatgttattg gggataatag cgttactatt
      841 gataactttt ttagttttaa agtttggtgg gacaaaaagt gaacaaccag aaaaaaaagt
      901 aactgaaaca ttaagtggat tagttgttga aaatcaggtc ttaaaagttc agttattaga
      961 ttttgtaagt aataaggatt ttgatgataa gtatcaagaa gtttcaatgg atattaaagc
     1021 agatgaagaa gtattaaact ataaaatatc aaatcgtcaa gtttttaata aagtgatgca
     1081 gttattaccg ccaggtgagg gctctccttt attgaataac agcagtgagg tacctactca
     1141 tgaagcgtat attcttgttt taactggtga tattgttgag tataaagata gtgaaggaaa
     1201 gagttcttat caaattgcta atgcacgtct tgattactat aaacaatcat tgttgttaga
     1261 gaatgattat gatagtgtgt atatcgcttc aattgatgga aaaaaagaaa aaatggtcaa
     1321 gattactgca tataaggaag cactatccag tccaagtgag tatatgacaa tgttacaatg
     1381 gtaaataaaa accttagcaa aataaaatgc taaggttttt tagtattatt caatgtttag
     1441 tccagcaaca aaggttagat caataccaaa cataatgccg gtattgggtt tatcgatgcc
     1501 gcctaaagtt ttatcaataa cggtccttgc tttttcaaca tcttcatttt ttaaaataaa
     1561 gaaaagtgtt ttgcttgttt cacggggatc ttcaagaaaa attcgtaatg attcgataat
     1621 aaaattatca ttacttttta atgtattaag catccctttg ctttcaatga tcgtccctcc
     1681 gttaatacca cttttttgta atgcggctaa gagatcatct aatttttcta ctttgtgtaa
     1741 aactaagaaa agtgcttgca tggaattcct cctttatgta catcctatta tatattataa
     1801 accatattat tgctctttgc attagtaaat gtttggttta taataaatag tatacaaata
     1861 agggggtata tttaaatgaa ttttgaattt tcaaatcgga tcagtggtgt taaggcatca
     1921 gcaattaggg aaattttaaa aatgatgaat gatccagaga ttattagttt tggtggtgga
     1981 aatccggcta gtgagacttt tcctgttgaa caattaaaaa gaattagtga tgatattttt
     2041 aatacttgtc ctaattctat tttagagtat ggaattactg aaggtgacgg aagctttaaa
     2101 aaagcagcaa tagaattttt atcacggcat gaaacagtag tgaaagacta tgatggtgtt
     2161 atggttactt ctggttcaca acaaatcatg gattttttat ctaaatgttt atgtaatgaa
     2221 ggggatctag ttgtttgtga aaatccaagc tttttagggg ctttaaatgc atttaaaagt
     2281 aatggtgcca aattagttgg aattgatatg gaagatgatg gaatcaatct tgaacagtta
     2341 gaagcagttt taagtggggt gcaaaaacca aaatttattt atttgattcc taatttccaa
     2401 aatcctactg gaataacaac ttctttagaa aaacgtcaag ctatttatac attagcaaaa
     2461 aaatatcaag tgctgattct tgaagataat ccatatggtg atttaagatt tagaaatgat
     2521 gcaattcctt caattaagtc attagatgat gaaggattag tagtttatgc agctagtttt
     2581 tcaaaaatta ttgcaccggg aatgcgtgta gctgttatga ttggtcatca agatctatta
     2641 gctaaatgca cagttgctaa acagacaaat gatgttcata caaatgcttg ggcgcaaagt
     2701 gtaatggctc gtttcttaaa tgaaacggat atggaggtac acttaaaaaa attacaggca
     2761 gtatatgagc aaaaatgtaa tttgatgttg gaagaaatga aaaaacaatt cagcccagat
     2821 tgtaaatgga caattcctga tgggggaatg tttatttggg tgactctacc agaacgaatt
     2881 gatatgccgg agtttgttaa aagagctgtt gaacataaag tagcagtagt tccaggaaat
     2941 gctttctatg atgatgataa taaaccatgt caatcgtttc ggatgaattt ttcaactcca
     3001 actaatgaac agattattaa aggggtcgct attttaggag cgttaatgca tgattaaaaa
     3061 tattgttttt gatatgggaa atgttatttt gcgttgggat ccgcactata ttgctagtaa
     3121 attatcaaat gatagtttag aacaagatgt gattgaaaga gagttatttg cttcaaaaca
     3181 gtggcaaaag ctagatcaag gattactcac cgtggaagaa gctcttaagg aattaaaaac
     3241 cgacaatccg cagttgatta gacatgcatt attgcattgg tatgattatt ttgagccttt
     3301 tgctgaaatg gtagcattag ttaaagaact aaaagagcaa ggttatcata tttatctatt
     3361 atctaattgc agtctccaat ttgatgatta ttaccaaaat atcgaagctt ttaaatattt
     3421 tgatgatttt tatatttctg cacgctacca attattaaaa cctgatcttg aaatcttcaa
     3481 acattttctt tcaaaattta atttaaaggc tgaggaatgt atttttattg acgatataaa
     3541 agctaatgtt gaaggtgcaa agattgctaa aatgcatggt tatcagcata atggagatat
     3601 caaaaagtta cgattatatc taaaaaaaca acatgattta ccaataaatt attaataatt
     3661 cttaaaaata ccatactttt caactgtttt ggttgacaat taaggggaaa atgctagaat
     3721 gctaatagac aaaataatcg cttatattgg gagaatttag atggataaac aagaggaaaa
     3781 tatgagtaac acaaacgata cgattgaatc aaagcaaaag tcagaaatga ctaaaaaagt
     3841 aagatcaggg gaaaaaacag gggtacaaaa aaagattatt attattggaa tcttagtatc
     3901 attattagtc attatttatt taattggaag tttttatttc aataagaagt ttttaagtgg
     3961 tacatacatt aatgatatca atgtcagtgg tttaacatta actgaagcga atgaaaagtt
     4021 agcggaacaa atagatgctc gttctttaaa attgattttt aatgatgaag caactgaaac
     4081 agtttcagga agcgaatgtg gagttcgcta taatagtaat aataatgtta agaatatttt
     4141 gaaaaagcaa aattcttttg ggtggattgg agcttatttt aataaatcga aaaatgtagt
     4201 taatgatcta gctgaagttg atgaaacagt tttaagagct aaggtggctt ctttgggaca
     4261 tcttcaagaa gaggtgcaag tggcaccggt cgatgctaaa gtggaatatc taaataatga
     4321 gtttaccatc accaacgagg tcaatggttc aacgattgat caagaaaaac taattagtga
     4381 aataaagtta gcctttagtg aaggtaaaga aagtttaaat ttaacggaga aaaaatgtta
     4441 tgtagaaccc gctgtaaaag ctaatgatgc gaaattacaa aatttattag atgcagctag
     4501 aaagtatgca tctgcggcga taacctataa aacacgttct ggagatgtag tgttagatgg
     4561 tagtactttg gttacttggt tatcaataga tgaaagtggt aattattatc gtgatgatgc
     4621 tgtatttaaa gaaaaagtaa ctgactttgt tggaagttta gcgaaaatta acagtgttgg
     4681 tggaagccga acttttgttg gggctaataa tcggacgatt acagttagcg gtggtaatta
     4741 tggtttaaga ctccaaaata gtaaggaaat aagcggttta ttagaagata tttatgccaa
     4801 taaagtgggt gtcagaacac ctgttacaac tggtcgtgaa gcttcttcgg ataatggtgg
     4861 attaggtgat acttttgttg aaattgattt atcagggcaa catatgtggt atcataaaaa
     4921 tggtcagatc gtattagagt cagatattgt ttctggtaca tacaatattg ctgatcgccg
     4981 tacaccagcg ggtgcctatt atttatataa taaggaaaga aatcgtgttc ttcggggaac
     5041 aaaattacct gatggaacat ggccatatga aactccagtt tcttattgga tgccttttaa
     5101 taaaggaatc gggttacatg attcttcaag ctggcgttct aagtggggtg gaactattta
     5161 tatgaacaat ggttctcatg gttgtattaa tttacctacg ggaattgctt cacaattata
     5221 taatagtatt gaagttaatt gtcctgtagt ttgttattat taataatgta aaacagtctt
     5281 aaaattgctg cgatctcaaa gaaagtttaa aagagtagcc ctaatccttt taaattattc
     5341 aaaacgtatc agtaaataag gctgttttta ttgtttaaat tattagtaat tagttatata
     5401 cttgaagaag agaataaagg agttaaatga tggaaaatta tgtaatagat tttgcaacta
     5461 aaatattaaa tattgatagt cctacaggtt attgcaaaga agttattgaa tttgttaaaa
     5521 atgaagttga acaattaggt tatcgtacag gaatgaataa taaaggaaat ctttatattt
     5581 atgttgatgg caagaatgat aaaacaattg gt
//
LOCUS       DAOMTA010000088         5588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_287_length_5588_cov_2.140069, whole genome shotgun sequence.
ACCESSION   DAOMTA010000088 DAOMTA010000000
VERSION     DAOMTA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_287_length_5588_cov_2.140069"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..545
                     /locus_tag="PLK68_03675"
     CDS             63..545
                     /locus_tag="PLK68_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRM89543.1"
                     /translation="MEAAFSNPKLVGCMLLVTAIFLLSIHKFGYKGKRTAKKLNLFDA
                     LRMGIFQLFALLPGISRSGSTLTGGMLGGLSQKAARDFSFFMFMPVSFGAIILKLKDF
                     LTSGTLGALWLPYLVAFIISGIVTYLALHLLFKLLDKKKLNVFSLYCLVVGILAVLFL
                     "
     gene            complement(580..741)
                     /locus_tag="PLK68_03680"
     CDS             complement(580..741)
                     /locus_tag="PLK68_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89544.1"
                     /translation="MIETAYYAQDNYAILKDILTAFDLYKKIIKIDGKEMNITDIIKI
                     NSKKNSFLK"
     gene            complement(1118..2311)
                     /locus_tag="PLK68_03685"
     CDS             complement(1118..2311)
                     /locus_tag="PLK68_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM89545.1"
                     /translation="MEKIKQQFYNMSLKKSLLLILSVALFLIIILSSTTVLLTSSLRQ
                     MILDNRTIHIAVDTAIISENITGTYEISPSNDEYQYSELANQDKFIYLSATVAMIALP
                     TLYLVLGTIFIVKIYYRLKLALPIEQLNIGIANLTNNNLDFQITYNSNDELGRLCSTL
                     EVMRNELIENNQKVWELLDQRKALTASVSHDLRTPITVLKGYLDYLLKNLPEKRVSDD
                     VLLTTLKSMAHSSARLERYVECIQDIQKIEDIEISKTMVVGKDFLEEINNDFMIIAKN
                     NHKDLVLEAKIVSKQLYLDKQIIFKILENLLNNAFRFANNGVKLTIMETVGYLEFVIQ
                     DDGPGFSKKDLENATTLFYSSQTNKGSFGIGLSISKILCEKHGGILKLLNNENGACAI
                     VQIKK"
     gene            complement(2299..2481)
                     /locus_tag="PLK68_03690"
     CDS             complement(2299..2481)
                     /locus_tag="PLK68_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM89546.1"
                     /translation="MNAGQIFDREKIYEVVWGFEANGDSAVIKEHIRKIRMKLARYSE
                     NEYLETVWGVGYRWKK"
     gene            complement(2650..2937)
                     /locus_tag="PLK68_03695"
                     /pseudo
     CDS             complement(2650..2937)
                     /locus_tag="PLK68_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3107..3481)
                     /locus_tag="PLK68_03700"
     CDS             complement(3107..3481)
                     /locus_tag="PLK68_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRM89547.1"
                     /translation="MKNVVKTDKAPGAIGPYSQGINVGDMYFFSGQIPLVPETGEMPE
                     GIEAQAHQSLKNVQGLLESQGLTFANVIKTTVFLDNMDDFVTVNDIYAQYFVEPFPAR
                     SAVEVAKLPKGALIEVEVIATK"
     gene            3640..4830
                     /locus_tag="PLK68_03705"
     CDS             3640..4830
                     /locus_tag="PLK68_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM89548.1"
                     /translation="MKYTFAARFNNITPSGIRAVLEKAAGPDMINFSPGFPDNDAFPA
                     NDIQKISQDVLKEDIYTILQYSRGTTYPPLKKALKAFFNRQEKIFTENDDLMVTSGSG
                     EGLEMAAKVFLNPGESIIVEDPTFVGALNGFISNDAKLLGVPVESDGMNLGLLEEAMQ
                     STPAPKLLYIIPTFQNPTCITTSLEKRRAIYDLCLKYNIIILEDNPYGTLRFKGKTIP
                     TLKSIDTEGIVVYCASLSKIISPGIRLGTIIANKEIIDKFNILKGASAGAVTNWSQHV
                     IARFLETVDMDKHLAHLQSVYGKKSTFMVEMMKKTFHPDVKFTTPEGGMFVWFELPKY
                     ADARTFLNRATTMHIAIVNEETFAVNRRDKMNGFRLSFTSATMEQIETGIAKLGKLTY
                     ELCK"
     gene            4891..>5588
                     /locus_tag="PLK68_03710"
     CDS             4891..>5588
                     /locus_tag="PLK68_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM89549.1"
                     /translation="MKRGLVLAGGGSKGAYQAGCIKALKELGYDFDLVTGTSIGALNG
                     LLVVQEDYNTLYRLWDEITISEVLEDPVNFNFSIDSMLSQTNLIKPFFKSYINEKGAD
                     ITPLKNILHALYNEEKAFNNKIDYGVVAVKYPKLTPIEITKEEMKPVPAIEYAIASAS
                     CFPAFPVHHIDKQGYIDGGYYDNLPISLALKMGADKIIAIELNQDATHDYFLDRPDII
                     FIRPSYDLGGFLDFG"
BASE COUNT         1864 a          945 c          798 g         1981 t
ORIGIN      
        1 tttgcggaca tttaagaaga tataaaaaga tagtcaaaaa gacatatata atttataaat
       61 aaattgaagc agctttttct aaccctaaat tagttggatg catgttactg gttacagcga
      121 ttttcttatt atcaatccat aaatttggct acaaaggaaa acggacagca aaaaaattaa
      181 acttattcga cgctttaaga atggggattt ttcagttatt tgcattactt ccaggaatct
      241 ctcgtagtgg ttctacccta acaggaggaa tgttaggggg attatcacaa aaggccgctc
      301 gtgatttttc attttttatg ttcatgcctg ttagttttgg agcaattatt ttaaaactaa
      361 aagacttttt aacatccggc acacttggtg ctttatggct tccttattta gttgctttta
      421 ttataagtgg aatcgttaca tatttggctc tacacttatt atttaaatta ctagataaaa
      481 agaaattaaa tgtcttttct ttatattgtt tagtagttgg tattttagcc gtattatttt
      541 tgtaaaacag tttaaaatga aatgtctctc cattaaagtt catttcaaaa aactgttttt
      601 tttactatta attttaatta tatctgtaat attcatttct ttaccatcaa ttttgattat
      661 ttttttgtat agatcaaagg ctgtaagtat atccttgaga attgcataat tatcttgagc
      721 ataataagca gtttcgatca tttattcact tctaagctac ctaatcttcc agtccaactc
      781 ttctattgca tcaagagcaa gctttttcgt tcgataatga cccacaattt agccctgaac
      841 aattctttaa atccttttag tggatcataa gataaaatta ttttacacgt tcggcctttt
      901 catcacattg aactattctc tttccaatat gctaaaaacc agttaatagc tgatgatgaa
      961 gttacaataa aaaaatacca ttgatttact ttaatcacaa gtaattcgat ggttattgat
     1021 aaatactttg atcctcacca aatcctagaa ttaccaataa agataattgg caaagttatt
     1081 tttactaaaa actattatta agtaagaatt ccatctctca ctttttaatt tgaacaattg
     1141 cacaagctcc attttcgtta tttaataatt ttagaatacc gccatgtttt tcacaaagga
     1201 tcttactaat cgataatcca attccaaaac ttcctttatt agtctgtgat gaataaaata
     1261 atgtagttgc attttctaaa tcctttttac taaaacccgg cccatcatcc tgaataacaa
     1321 attcaagata acccacagtt tccataatcg ttaacttaac cccattatta gcaaagcgga
     1381 aagcgttatt taacaaattt tctaatattt taaaaataat ctgtttatca agatataatt
     1441 gtttagacac tatttttgct tccaatacta aatctttatg attattctta gcaataatca
     1501 taaaatcatt attaatttct tctagaaaat ctttgccgac aaccattgtc ttactaattt
     1561 caatatcttc tattttttgg atatcttgaa tacattcaac atatcgctct aaacgagctg
     1621 atgaatgagc catacttttt aaggtcgtta ataaaacatc atcagatact ctcttttctg
     1681 gaagattttt taataaataa tcaaggtatc ccttcaatac tgtaatcggg gttcttaaat
     1741 catgtgatac tgatgccgtt aaagcttttc gctgatctaa aagttcccat actttttggt
     1801 tattttcaat cagttcatta cgcattacct ctaaagtact acataaccga cctaattcat
     1861 cattagaatt ataagttatt tgaaaatcaa gattattatt ggttaaatta gcgattccta
     1921 tatttaattg ttcaatcggc aacgccagtt ttaaacgata gtatatcttt acaataaaga
     1981 ttgtccctaa aacaagatat aatgttggta atgcaatcat cgctactgtc gcactaagat
     2041 aaataaattt atcttgatta gccaattcac tatactgata ctcatcattt gagggcgaga
     2101 tttcatacgt acctgtaata ttttcactaa taatagcggt atctactgca atatgaatag
     2161 tccgattatc aagaatcatt tgtcgtaatg atgaagtcaa caaaactgtt gtagaagaca
     2221 aaataataat taaaaataaa gctacactta atattaataa taatgatttt tttaaagaca
     2281 tgttataaaa ttgctgtttt attttttcca tcgataccct acgccccaca ctgtttctaa
     2341 gtattcattt tcactatacc gggctaattt cattcggatt ttacgaatat gttctttaat
     2401 tacagcacta tcaccattag cttcaaagcc ccatactact tcataaattt tttcacgatc
     2461 aaatatttga ccagcattca tagataataa tttaataatt tcaaactcct tattagaaaa
     2521 atcaattttt ttatttttaa taattacggt tctttgacta taatcaataa tcagttccct
     2581 agtaaaatgt cctcgtgatt tatgggagct gcgtttttca cgacgtaaat gagcttgaac
     2641 tcttgcgaaa agttcatcca tcgaaaatgg ttttgtaata taatcatcgc ctcccagcat
     2701 taaaccattg atcatatccc gactagaaat tcgcgctgtt aaaaatataa tcgggcaatt
     2761 cacatgttcc cgaatcatcg tacataattc taaaccattc atctccggca tattgatatc
     2821 taataaaata aggtcaggta aaactgataa ttgttttaaa gcttcattag catcattagc
     2881 taaataaact aaatatcctt cagattgaaa ttgtaatgcc agcatttcta aaatgtctct
     2941 ttcatcatca gaatcttata cattgttttt tccccctata gttattttta ttatacttaa
     3001 gtttgtcaac ttttggtcaa cttcataaaa aaacttaagt attatgcatc aacctgcaat
     3061 cgttagattt ttaaggttca atacaataac ttaagctctt ttataattat ttagttgcaa
     3121 taacttctac ttcaattaaa gcccctttag gtaatttagc tacttctaca gcactacgag
     3181 ctgggaatgg ttcaacaaaa tattgagcat aaatgtcatt tacagtaaca aaatcatcca
     3241 tattatctaa aaatacagtt gttttaataa catttgcaaa tgttaaacct tggctttcta
     3301 ataatccttg aacattcttt aatgattgat gagcttgtgc ttcaatccct tcaggcattt
     3361 cccctgtttc tggtactaat ggaatttgtc ctgaaaagaa atacatatca ccaacattaa
     3421 ttccttggct atatggcccg atagctccag gtgctttatc tgtttttact acatttttca
     3481 tctttaatat cctccttaaa tcattataaa caaataatca ctatttttaa agttttatta
     3541 atgaaaatat tgccattttt atcactgtga taatttatta ttgacttatt acaattcttt
     3601 gctataatgt tgacaataca aaaggaggat agcacgacta tgaaatatac atttgcagct
     3661 agatttaata acattactcc aagtggtatt cgcgctgtac tagaaaaagc agcgggaccc
     3721 gatatgatca acttctcacc gggctttcct gataacgatg cttttccagc taatgatatt
     3781 caaaagattt cacaagatgt tcttaaggaa gatatttata ctatcttgca atattccaga
     3841 ggtaccactt atccaccatt aaaaaaggca ttaaaagctt tctttaatcg tcaagaaaaa
     3901 atttttacag aaaatgatga tctaatggtt acttccggct caggagaagg actggaaatg
     3961 gctgccaaag tatttttaaa cccaggagaa agtatcatcg tcgaagatcc tacatttgtc
     4021 ggagcgctga atggatttat tagcaatgat gctaaactgc ttggtgtgcc tgttgaaagt
     4081 gatggaatga atttaggatt attagaagaa gcaatgcaaa gcactcctgc ccccaaacta
     4141 ctatatatta ttcctacttt ccaaaatcct acctgtatca ctacttcact agaaaaaaga
     4201 agagctatat atgacttatg tttaaagtat aatatcatca ttcttgaaga taatccatat
     4261 ggcacattgc gttttaaagg taaaaccatc ccgactttaa aatcaatcga tactgaagga
     4321 atcgttgttt actgtgcaag cctatcaaaa atcatttctc cgggtatccg gcttggaaca
     4381 ataattgcga ataaagaaat tattgataaa tttaatattc ttaaaggggc tagtgctgga
     4441 gccgtaacta attggtcaca acatgtaatt gcacgctttt tagaaacagt tgatatggat
     4501 aagcatcttg ctcatttaca atctgtatat ggcaagaaaa gtacatttat ggttgaaatg
     4561 atgaaaaaaa cttttcatcc tgatgtaaaa ttcactaccc cagaaggtgg tatgttcgta
     4621 tggtttgaac ttccaaaata tgctgatgct aggacttttt tgaatagagc taccactatg
     4681 catatcgcaa ttgtaaatga ggaaactttt gccgttaaca gacgtgataa aatgaatggt
     4741 tttagattat cttttactag tgcaacaatg gagcaaatag aaacaggtat tgctaagtta
     4801 ggtaaactaa catatgaatt atgtaaataa atccactgat tggtggattt ttatttgttc
     4861 gcgcgtataa ttgaaatgag gtgactaaaa atgaaacgtg gattagtttt agcaggcgga
     4921 ggttctaaag gagcttatca agccggctgt attaaagcat taaaagaatt gggctatgat
     4981 tttgatctag taaccggaac ttcgattgga gctctcaatg gcctattagt cgttcaagaa
     5041 gattataaca ctctttatcg attatgggat gaaatcacaa ttagtgaagt tttagaggat
     5101 cctgtcaatt ttaatttttc aattgactca atgctttcac aaaccaattt aatcaaacct
     5161 ttttttaaat catatatcaa tgaaaaaggt gctgatatca ctcctttaaa aaacattctc
     5221 catgctcttt ataatgaaga aaaagccttt aataataaaa tagattatgg tgttgtagct
     5281 gtaaaatatc ctaaattgac tcctatcgaa ataactaaag aagaaatgaa gcctgtacca
     5341 gccattgaat atgcaattgc ttcagcttca tgttttcccg cttttccagt gcatcatatt
     5401 gataaacaag gttatatcga tggtggttat tatgataatc ttcctatttc gctagcgtta
     5461 aaaatgggtg ctgataaaat tattgcaatt gaattaaatc aagatgctac tcatgattat
     5521 ttcctcgatc gtcctgatat catatttatt cgtcctagct atgatcttgg cggatttctt
     5581 gattttgg
//
LOCUS       DAOMTA010000089         5494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_300_length_5494_cov_2.600110, whole genome shotgun sequence.
ACCESSION   DAOMTA010000089 DAOMTA010000000
VERSION     DAOMTA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_5494_cov_2.600110"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLK68_03715"
     CDS             <1..650
                     /locus_tag="PLK68_03715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM89550.1"
                     /translation="EMKIKYCIVFEEKEQLPKLYEQYYYLMHENQKESIDFCLNSVLY
                     KIELFKAMEEKKDIVKESRLDDLTKIYNRRYFHYKYSEFKNKSRLLGIIIFDLDHFKE
                     YNDSLGHLTGDQILKDFADSLQQDDDRIISCRFGGDEFICICVDCNEQMIISFIEQVY
                     SKFELYGYDKITISSGYYNSFSNCLSKEELINNADYYLYYVKENGKNGYYGFSIE"
     gene            745..2298
                     /locus_tag="PLK68_03720"
     CDS             745..2298
                     /locus_tag="PLK68_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM89551.1"
                     /translation="MSILSVENVSHDFGGRTILENASFRLLNGEHIGLVGANGEGKST
                     FLNIITGKIAPDSGKIEWCKRISTGYLDQHTALHPGKTIYETLQDAFKYYYDLEAEML
                     SMYEQMADCDDETMNQLMEEVGEIQEILDHGGFYTIDVKIKEVAGGLGLNDIGLDKPV
                     DALSGGQRSKVLLTKLLLENPMILILDEPTNYLDEQHINWLINFLKNYDNAFILVSHD
                     VSFLNQVINIIYHLEDGVLTRYKGDYDYYLQQVELKKRQLAADYQKQQKEIADLEDFI
                     ARNKARVATRNMASSRQKKLDKMNLISKPKEKIKPTFSFIEARTPGKVLFDCKDLVIG
                     YDSPLTKEMNLVFERNKKVAIKGVNGLGKTTLLKTLIGMQKPYSGTVEKDPFVEIGFF
                     KQEESAVSQTALDYLWEEFPDRNNGEIRAMLAKCGLTTDHIESLMKVLSGGENAKVRL
                     CKIMNREANVLVLDEPTNHLDVDAKDSLQSAIKAFKGAVILVSHEPEFYLPIVDEVIN
                     LEECSTKII"
     gene            2383..3462
                     /locus_tag="PLK68_03725"
     CDS             2383..3462
                     /locus_tag="PLK68_03725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM89552.1"
                     /translation="MITISVCMIVKDEEMVLERALKSVERIADEIIIVDTGSTDRTKK
                     IALKYTDKVYDFVWCDDFAKARNYSFSKATMDYCMWLDADDIISDSDQEGLINLKKTL
                     SKDTSVVMMKYNAEFDQNNKPTFTYYRERLLRRKDNFYWEGFIHEVISPRGKVLYSDL
                     AITHSKVKNSDVNRNLRIFREKLNEGVMFSPREIFYYARELFYHQLYQEAVAEFKRFL
                     NTKKGWKENNISACQFLGYCFYNLGDMQEAITWFLNSLCYDLPRAEICCDLGKYFFEL
                     KQYEQSIYWSAVALSCERDDTSGAFISPDCYDYIPYMQLCLCYDKLSNYKTAKYYNEL
                     AGKCKPNDPSYLHNKLYFNRIENDE"
     gene            3719..>4222
                     /locus_tag="PLK68_03730"
     CDS             3719..>4222
                     /locus_tag="PLK68_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HRM89553.1"
                     /translation="MKDCNYNGCIWCPCCRCRPPIIIRCPTGPTGATGATGPTGVTGV
                     IGPTGATGPTGVTGMIGPTGATGATGPTGPTGATGEDGATGATGPTGSTGATGEDGAT
                     GPTGPTGPTGVTGATGSTGLTGATGEDGATGPTGPTGSTGPTGATGEDGATGATGPTG
                     PTGATGED"
     assembly_gap    4223..4322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4323..5159
                     /locus_tag="PLK68_03735"
     CDS             <4323..5159
                     /locus_tag="PLK68_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium glycoprotein BclB-related protein"
                     /protein_id="HRM89554.1"
                     /translation="GATGATGPTGATGATGPTGPTGATGEDGATGPTGATGATGPTGA
                     TGEDGATGPTGPTGSTGATGSTGPTGTNGANGDRGPTGPTGITGATGATGSTGPTGST
                     GAAGASAIIPFASGLPVSLTTIAGGLVGTPAFIGFGSSAPGISIIGNTIDLTNPSGTL
                     TNFAFTMPRDGVITSIDVFFSTTAALSLVGSTITIEAKLYESIAPNNTMTVVPGTTVT
                     LTPSLTGVISIGTISKGILTGLNINVPAGTRLMLVLTARASGLSLVNTVAGYVSAGVA
                     ID"
BASE COUNT         1929 a          691 c         1134 g         1640 t
ORIGIN      
        1 tagaaatgaa gataaagtat tgtattgttt ttgaagaaaa agaacagttg cctaaattat
       61 atgaacaata ttattatttg atgcatgaaa atcaaaaaga aagtattgat ttttgtttaa
      121 atagtgtttt atataaaatt gaactattta aagcaatgga agaaaaaaaa gatatcgtta
      181 aggagtctag actagatgat ttaactaaga tttataaccg acgatatttt cattataaat
      241 atagtgagtt taaaaacaaa agtcgactat taggaattat tatttttgat ttggatcatt
      301 ttaaagaata caatgatagt ttaggtcatt taactggtga tcaaatatta aaggattttg
      361 ctgatagttt acaacaagat gatgatagaa taatttcttg tcgttttggc ggtgatgaat
      421 ttatttgtat ttgtgtagat tgtaatgagc aaatgataat tagttttatt gaacaagttt
      481 atagtaaatt tgagttatat gggtatgata aaatcactat ttcatctgga tattataatt
      541 cttttagtaa ttgtttatct aaagaggaat taattaataa tgcagattat tatttatatt
      601 atgttaaaga gaatggaaag aatggatact atggcttttc aattgagtga ttgtttagac
      661 tcgacagtta gtaattttaa tgtataatat agttgaatta ggaagatgat aatcttcctt
      721 taatattgca agttgaggta gaaaatgagt atattaagtg tggaaaatgt cagtcatgat
      781 tttggtggaa gaactatttt agaaaatgca agttttagat tattaaatgg cgaacatatt
      841 ggtttagtag gtgctaatgg tgaggggaaa tctacttttt taaatattat tactggtaag
      901 attgctccgg attcaggaaa gattgaatgg tgtaaaagaa tttcaacagg ttatttagac
      961 caacacacag ctttacaccc aggaaaaact atatatgaaa cattacaaga tgcttttaaa
     1021 tattattatg atctagaagc ggagatgtta tcgatgtatg aacaaatggc tgattgtgat
     1081 gatgaaacga tgaatcagtt gatggaagaa gttggtgaaa ttcaagaaat tttagatcat
     1141 ggcgggtttt atacaattga tgttaaaatc aaagaagttg caggtggtct agggttgaat
     1201 gatattggtt tagataaacc ggttgatgcg ctatctggtg ggcaacgttc aaaagtttta
     1261 ctaactaaat tattattaga gaatccaatg attttaattc ttgatgaacc aactaactat
     1321 ttagatgagc agcatatcaa ttggttaatc aattttttaa agaattatga taatgctttt
     1381 attttagtca gtcatgatgt tagtttttta aatcaagtta ttaatattat ttatcattta
     1441 gaagatggtg tattgactcg ttataaaggt gattatgact actatttaca acaagttgaa
     1501 ttgaaaaaac gtcagttagc tgctgattat caaaagcagc aaaaagaaat tgctgatctt
     1561 gaagatttta tcgcacgtaa taaagcacgg gtagctactc gtaatatggc tagttcacga
     1621 caaaagaaat tagataagat gaatcttatt tctaaaccaa aggaaaaaat aaaaccgact
     1681 ttttctttta tagaagctag aacaccaggt aaagtattat ttgattgtaa ggatttagtg
     1741 attggatatg atagtccttt aacgaaagaa atgaatcttg tttttgaaag aaataagaaa
     1801 gttgctatta aaggtgtcaa tggtttggga aagacaactc tactaaagac cctgattggg
     1861 atgcaaaaac cttatagtgg aacagtagaa aaagatccat ttgttgaaat tggttttttt
     1921 aaacaagaag aatctgcagt ttcccaaact gcattagact atttgtggga ggaatttcca
     1981 gatcgaaaca atggtgagat tagagctatg ttagctaagt gtggtttaac aactgatcat
     2041 attgaatctt taatgaaagt tttatcgggt ggagaaaatg ccaaagttag attatgtaag
     2101 attatgaatc gtgaagctaa tgttttggtg ttagatgaac cgacaaatca tttggacgtt
     2161 gatgctaaag attctttaca aagcgcaatc aaggctttta aaggagctgt tattttagtt
     2221 agtcatgaac cggaatttta tttaccaatt gttgatgaag taattaattt agaagaatgc
     2281 tcaactaaga ttatttagaa atgttcttta tttagaacat tttttgtttt aaatattttc
     2341 acattttaag ggttgttgac atatattata gtggtgaaat atatgataac tattagtgtg
     2401 tgtatgatcg taaaagatga ggaaatggta ttagagagag ctttaaaatc ggttgaaagg
     2461 attgcagatg aaataatcat tgtggatacc ggatcgactg atcgaactaa aaagatagca
     2521 ttgaaatata ctgataaagt atatgatttt gtatggtgtg atgattttgc taaagcaagg
     2581 aattattcat tttcaaaggc aactatggat tactgcatgt ggttagacgc ggatgatatt
     2641 atttcagata gcgatcaaga aggtttgatt aatcttaaaa aaactttatc aaaagatact
     2701 tctgtagtta tgatgaaata taatgcagaa tttgatcaga ataataagcc gacatttacg
     2761 tattatcgtg aaagattatt aagaagaaag gataattttt attgggaagg atttatacat
     2821 gaagtaataa gtcctagagg taaggtgctt tatagtgatc ttgcaattac acatagtaag
     2881 gtgaaaaata gtgatgttaa tcgtaattta agaatattta gagaaaaatt gaatgaaggg
     2941 gtaatgttta gtccacgaga aattttctat tatgctcggg agctgtttta tcatcaattg
     3001 tatcaagaag cagtagctga atttaaaaga tttttaaata ccaagaaggg atggaaagaa
     3061 aataacattt ctgcctgtca atttttaggc tattgttttt acaatcttgg tgatatgcaa
     3121 gaagcaataa cctggttttt aaatagtctt tgctatgatt tgccaagagc agagatatgc
     3181 tgtgatttag gtaagtattt ctttgaattg aagcagtatg agcaatctat ctattggagt
     3241 gcagtagcat tatcgtgtga gcgtgatgat acatctggag catttatatc tccagattgt
     3301 tatgattata ttccctatat gcaattatgt ttatgttatg ataagctaag taattataaa
     3361 acagcaaaat attacaatga attggcagga aaatgtaaac caaatgatcc atcatatttg
     3421 cataataaat tgtattttaa tcgtattgaa aatgatgaat agaagtaaaa aaagatttaa
     3481 aagggactaa tccttaataa aggagtaagt ccttttatta taatatgata aactattatt
     3541 ttttataagg cgttaagggt atacgattta catatgtatc tagtaacttt tttatttaga
     3601 taattacttt agaagtttaa tgaaaaaatt agtcttttat tttttatgag attattgaca
     3661 cagtagttat taaaatattt gcgtatatta tattagatca cataaaggag gtgataagat
     3721 gaaagactgt aattataatg gttgtatatg gtgtccatgt tgccgatgca ggccaccaat
     3781 tattattaga tgtccaaccg gaccgaccgg agcaacagga gcgacaggac caaccggagt
     3841 aacaggcgtg ataggaccga ctggagcaac aggaccaacc ggagtaacag gcatgatagg
     3901 accgaccgga gcaacaggag cgacaggacc aaccggaccg accggagcaa cgggagaaga
     3961 cggagcaaca ggagcgacag gaccaacagg atcaaccgga gcaacaggag aagacggagc
     4021 aacaggacca acaggaccaa caggaccaac cggagtaaca ggagcgacag ggtcaaccgg
     4081 actgactgga gcaacgggag aagacggagc aacaggacca accggaccga ctggatcaac
     4141 aggaccaacc ggagcaacgg gagaagacgg agcaacagga gcgacaggac caaccggacc
     4201 gaccggagca acgggagaag acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnggagcgac aggagcgaca ggaccgaccg gagcaacagg agcgacaggg ccaaccggac
     4381 cgaccggagc aacgggagaa gacggagcaa caggaccgac tggagcaacg ggagcgacag
     4441 gaccaaccgg agcaacggga gaagatggag caacagggcc aaccggaccg actggatcaa
     4501 caggagcgac agggtcaacc ggaccgactg gtacaaatgg tgcaaacgga gatagaggac
     4561 cgacaggacc aactgggata acaggagcaa ccggagcaac agggtcaacc ggaccgactg
     4621 gatcaacagg agcagctgga gcttcagcaa taataccatt tgcatctggt ttacctgtat
     4681 ctttaacaac tattgctggt ggtttagtag gtacccctgc atttataggg tttggtagct
     4741 cagcaccagg aatatcaatc attggtaata caattgattt aaccaatcca tctggcacgt
     4801 taacaaactt tgcttttaca atgccaagag atggagtcat cacttcaatc gatgtattct
     4861 tttcaacaac agcagcatta tcattagtgg gaagtacaat tacgattgaa gcaaaattat
     4921 atgaatcaat tgctccaaac aacactatga ctgtagtgcc tggaacaact gtaacattaa
     4981 ctccttcact aacaggtgta atttctatag gaacgatttc taagggaata ttaactggct
     5041 taaatattaa tgttccggcg ggaacaagat taatgcttgt tttaactgca cgggcatctg
     5101 gattgagttt agtcaacact gttgcaggtt atgtaagcgc aggtgtagca atagactagc
     5161 taaaaaagga gtctctaata acttataata aaaaatatag tttttgtgaa atgtaatatt
     5221 tatgattata ggtatctcgc agttaaaaag ttcttattaa aatgaagtgc accccaaagt
     5281 ttgatatata cttggaatct tggacatgta tattcaaatg aaaggggtgc atcttaatat
     5341 gtaaaaatta tttaaataat tcgtttataa aaatattttt aaaatcataa aaagtgtttt
     5401 atagataaga atataagact atgtcaagta ttatactatt taaataatta caatctaatt
     5461 aaagggagta gtttctattc aattcagaaa ttac
//
LOCUS       DAOMTA010000090         5493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_301_length_5493_cov_2.617323, whole genome shotgun sequence.
ACCESSION   DAOMTA010000090 DAOMTA010000000
VERSION     DAOMTA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_301_length_5493_cov_2.617323"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..1230
                     /locus_tag="PLK68_03740"
     CDS             436..1230
                     /locus_tag="PLK68_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89555.1"
                     /translation="MKREGIHGLIQYLNKTTNNATNTQVAKAIFTNRDKINEISLEKL
                     AGDNYLSQASVSRFIKNQGYKNINEYRWDFIAGQQMLRLNAFNNKKVIISKNNEEIKN
                     DVKQSLQNAFNDIDKLDMTALERLVKIINNYKQVLFIGSEFSLANIYLVQLEMVQYGI
                     NAYSYNDPIIMAENLRSLKEDTLIICISTSGQWYNAPSTKEIRDILFSLNNPKILLTC
                     ITQHTDEAKFDYIYKFGNQRNDEISSYIQLTYFIPIFRNMYIRYID"
     gene            1364..3250
                     /locus_tag="PLK68_03745"
     CDS             1364..3250
                     /locus_tag="PLK68_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRM89556.1"
                     /translation="MKKITKALLALTLSLLMLAAPISVNQLYANENILTEVTSDTINN
                     YTYYEYDSEADGYTSERSNILTPIYYIFAGKQDLTSADKLIEEIGLLDNVHEWAGKVY
                     IINPISTQYNNDDVTAFKKLAGTGVSNIKVIGIDEGATFVNNYISQNCYFIAGMMVYG
                     GTMNSDLTYNVPIPAYLSSTATSAVSYYKQANQTDQSQSFNNYTIYQNSTNPLQIVVN
                     SKTDETLKNAFDNAWETVFSKNYRQHNETTEFYNMPVTDTNLANAEQPYKLIETPIFD
                     RLGIIHNQEINQTVSNMPGKYTWFEYLPNQVIDTKKDSVPLVLTLHGNGNDPRVQADS
                     SGWIELAAKENFIVVSPEWQDASVNFSKCDGLGDEGIINLIDDLKIKYPQIDRSRVYV
                     TGLSAGGAESLLLGVKNSETFAGVGAVSGVNLYSEAITELTNDYKGHETPLLYICGDH
                     DFFQMIPVDGSSQYGTSQLYGFSIWAEDSNTHIYSALQAYQKINDLTVTDMNMDLNPY
                     YGIKLDNQQWTKLGEKDMYTGTLSNNNGVVMELAAIKDHAHWNYKPEAQYIWNFFKNY
                     QRDLLTGELIFVNNGSNTTTVIDKKDDLTTSVKTGDEVEFEYLGILSVITITTFIYFK
                     KKIA"
     gene            complement(3381..4577)
                     /locus_tag="PLK68_03750"
     CDS             complement(3381..4577)
                     /locus_tag="PLK68_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRM89557.1"
                     /translation="MSKKDIVKYIIDEYGVTSPNDITNALKDLLGETLQEMLDTEFDE
                     HMGYDKYDQTAKKDNYRNGSSKKTVKTSQGHIDINIPRDRNASFDPVIIEKYNRDISD
                     IDNKIINLYARGMSTRDISESIKDIYGVDVSAPMISKITDKIIPAALEWQNRPLHTIY
                     PIVFIDCVHFNVKTENMVVKKAAYIVLGVTEDGYKEVLGIWVGENETAKFWLSVLTDL
                     KNRGVKDILIICSDGLTGIKQAIESAYPGTIQQRCIVHLIRNSCKYLSYKDRKEYCHD
                     LKRVYSANTEQSALENLDRCQEKWGNKYPYAFKVWRDNWNEVCSMFNYVPELRKIMYT
                     TNAIESLNSAFRKFTKVRTVYPTDESLFKSLYLAQDKITSKWNVPYANWGVIYSSLQI
                     IFEGRV"
     assembly_gap    4642..4651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4832..5309)
                     /gene="tnpA"
                     /locus_tag="PLK68_03755"
                     /pseudo
     CDS             complement(4832..5309)
                     /gene="tnpA"
                     /locus_tag="PLK68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535475.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1842 a          916 c          782 g         1943 t
ORIGIN      
        1 aatccgttaa atcaatctat tttaaccaaa caagttctcg tttatataaa taataaatca
       61 tggataaaag aaattgatta aaaacgtcat ttatttgaat tgtttaaaaa tgaattgaac
      121 tataaatatc tgttctatat gatttatgat agctcaaatg atgctcttat caaacaatta
      181 tatcaaaagc tctctaaata cattaatcta actataagct cctcgtcgaa cgtgatgatg
      241 ggcttccatc aacaattgaa ataattttaa cagaccatta aaaactcttc ttttgaaatt
      301 actatctctt gtgaactggt aataatcaag ttgattcaaa ataattattg acccaaaata
      361 ttacctagtt aataatgttt attcaaaaac aattaaatat ttcattttta tgctatactt
      421 ttattggtga taaaaatgaa aagagaaggt attcatggtt taattcaata tcttaataaa
      481 actactaata atgcaactaa tactcaggtt gctaaagcaa tctttactaa tcgtgataaa
      541 atcaatgaaa tcagtcttga aaaactagcc ggtgacaatt acttgtctca agcttcggtt
      601 agccggttta taaaaaatca gggttataaa aatatcaatg agtatcgttg ggatttcatt
      661 gctggacaac aaatgctgcg acttaatgct tttaataata aaaaagtgat tattagtaaa
      721 aataacgaag aaattaaaaa tgatgtaaaa caatcattac aaaatgcttt taatgatatt
      781 gataagcttg atatgacagc acttgaaaga ttagttaaaa taatcaataa ttataaacag
      841 gttcttttta ttggttctga attttcattg gctaatattt atctagttca attagaaatg
      901 gttcaatatg gtatcaatgc ttatagttat aacgatccca ttattatggc agaaaatctt
      961 aggtctttga aagaagatac cctaattatt tgtatcagta ccagtggtca atggtacaat
     1021 gctccttcaa ctaaggaaat tagagatatc ttattcagtc ttaataaccc taaaattctt
     1081 cttacctgca ttacacagca tactgatgaa gcaaagtttg attatatcta taaatttggc
     1141 aatcagcgta atgatgaaat tagtagttat atccagctta cttattttat tccaatcttt
     1201 agaaatatgt atattcgtta catcgactag ttcattcagt ttttgaataa actagtttta
     1261 ttttgaattg atcgttcaaa gataattatg ttttattatt gtaagcgctt gtataatttt
     1321 aatgtaactt aaaagttata aatataaaat caggggtgga acaatgaaaa aaataacaaa
     1381 agcattatta gcactaacat tgtcactatt aatgttagca gctccaattt cagttaatca
     1441 attatatgca aatgaaaaca ttttaactga agtaactagt gacacaatca ataattatac
     1501 ttactacgaa tatgatagtg aagcagatgg ctacacctca gaacgtagta atatcttaac
     1561 acctatttat tacatttttg cgggaaaaca agatcttact agcgctgata aattaattga
     1621 agaaatcggt cttctcgata atgttcatga atgggcaggg aaagtatata ttattaatcc
     1681 tatatcaacg caatataaca atgatgatgt cacagccttc aaaaaattag ccggcactgg
     1741 tgtcagcaac attaaagtga tcggaattga tgagggtgcc actttcgtca ataattatat
     1801 tagtcaaaat tgttacttta ttgctggaat gatggtatat ggcggtacaa tgaatagcga
     1861 tttaacttat aacgttccta taccagcata tttaagttca acagcaacat cagctgttag
     1921 ttattacaaa caagctaatc aaactgacca atctcaatca tttaacaact acacgatcta
     1981 tcaaaattct acaaatccat tacaaatcgt ggtaaattca aaaacagatg aaacattaaa
     2041 aaatgcattc gataatgcct gggaaacagt tttttctaaa aactatcgtc agcataatga
     2101 aacaaccgag ttctataata tgcctgttac tgatacaaat ttagctaatg ctgagcaacc
     2161 gtataaacta attgaaacgc caatttttga tcgccttgga atcattcata atcaagagat
     2221 taatcaaact gtctcaaata tgcctggaaa atacacttgg tttgaatatt tacctaatca
     2281 agtaattgat actaagaaag attctgttcc tttagttctt acgcttcatg gaaacggtaa
     2341 tgatcctcgg gttcaagccg attcttctgg ttggattgaa ttagctgcaa aagagaactt
     2401 tatcgttgta tccccagaat ggcaggatgc tagtgtcaat ttttcaaaat gcgatggttt
     2461 aggggatgaa ggaattatta acttaattga tgatttgaaa attaaatatc cacaaatcga
     2521 cagatcacga gtatacgtta ccggcttatc agcaggcggt gccgagtcat tattattagg
     2581 ggtcaagaat agtgaaactt ttgcaggggt tggggctgtt tctggtgtca atctttatag
     2641 tgaagcaatc actgaattaa ccaatgatta taaaggacat gaaacacctt tattatatat
     2701 ttgtggtgat catgatttct ttcaaatgat tcctgttgat ggttcaagtc aatacggtac
     2761 ttcacaatta tatggtttta gtatttgggc tgaagacagt aatacacata tatatagtgc
     2821 cctacaagca tatcaaaaaa tcaatgactt aaccgtgacg gatatgaata tggatttaaa
     2881 cccttattat ggcattaaat tggataatca gcaatggaca aaacttggtg aaaaagacat
     2941 gtatacagga actttgtcta acaataacgg tgtagtaatg gaacttgctg caattaaaga
     3001 tcatgctcac tggaactata aaccagaagc ccaatatatt tggaatttct ttaaaaacta
     3061 ccaacgtgat ctactaactg gtgaactgat ttttgtcaac aatggtagca atactacaac
     3121 agttattgat aaaaaagatg atttgacaac ctctgtaaaa acaggagatg aagttgaatt
     3181 tgaatatcta ggtattctat ctgttattac gataactact tttatctatt ttaaaaagaa
     3241 aatagcataa taaaagagcc tgtaaataat tttgtgtaaa gtcaaatttc attagtagaa
     3301 tgggtagtaa ttacattgag taatcatgta attactattt tttataatgt tatcatagaa
     3361 atattcgttg tcaatgaact ctatactctt ccttcaaaaa taatttgaag acttgaatat
     3421 atcactcccc aattggcgta aggtacattc cattttgagg ttattttatc ctgtgccaaa
     3481 tataatgatt taaataaact ttcatctgtc gggtatactg ttcgtacttt ggtaaacttt
     3541 ctaaaagcac tgttcaagct ttctattgca ttcgttgtgt acatgatctt tcttaactct
     3601 ggaacataat taaacatact gcatacctcg ttccagttat ctctccagac tttaaatgca
     3661 tacgggtatt tattacccca tttttcttga cacctgtcca ggttttctaa tgcactctgt
     3721 tctgtattag cactgtatac tctctttaaa tcatgacagt attcttttcg atctttataa
     3781 cttaaatact tacatgaatt tctaattaaa tgaacaatac agcgttgctg tatcgtaccg
     3841 ggataagctg attcaatcgc ctgtttaata cccgttaatc catcggaaca tataattaaa
     3901 atatctttta cacctcgatt ctttaaatct gtaagtactg acaaccagaa tttagctgtc
     3961 tcattttctc caacccatat acccagtact tctttatagc cgtcttcagt tactcccaaa
     4021 acaatatagg ctgctttttt tacaaccatg ttttctgttt ttacattaaa gtgaacgcag
     4081 tcaataaaaa ctataggata tatagtgtgt aatggcctgt tttgccactc taaagctgca
     4141 gggatgattt tatcagttat tttactaatc attggtgcac taacatctac accatagata
     4201 tctttaatag attcactaat atctcttgta gacatccctc tggcatacag attaattatt
     4261 ttattatcaa tatctgatat atctctatta tatttttcaa taatcacagg atcaaaagat
     4321 gcatttctat ctcgtgggat attaatatca atatggcctt gtgaagtttt tacagtcttt
     4381 ttagaactgc cattacgata attatctttt ttagcagttt ggtcatattt atcatatccc
     4441 atatgttcat caaattcagt atcaagcatt tcctgaagag tttcacctaa taaatctttt
     4501 aaagcattag taatatcatt tggtgaagta acaccatatt catcaataat atatttaaca
     4561 atatcttttt tactcataat aaaaatcctc ctttggtaga tgatatcatt ctaccaatga
     4621 aggattgttt acacaaatta tnnnnnnnnn nctaacaata attcttaaaa aattctaaat
     4681 aaaaaccacg cctaaaagac gtggttttct ctgaggctat aagcctatgt tgcttgctcg
     4741 cgtctcaagg cgctgctttc tcttcgttca agctatcgct tttgactctc gccatccctc
     4801 aaagggattt atttcctcct ttctcctttt gttatcttct tctctctttt ttgttttctt
     4861 tcttcatttc tttatcttca aatggatttt gatattcttt tacactcaat ttatccattg
     4921 ctatatcatg tccctcttgt tcccttatat attttgcgac tgtttgatca tttagtccta
     4981 ctgtactcac ataatatcct tctgaccaaa aatgtctatt tccatattta tatttcaaat
     5041 ttgcatgtcg atcaaatatc attaatgctg acttaccttt caaatacccc ataaatgatg
     5101 atacacttaa ctttggtggt atcattacca acatatgtac atgatctgac atcaaatgcc
     5161 cctctatatt gacataatct tattattatt tctcttatat catttctaag ttgattatat
     5221 atcacttttc gtctatactt tggtgtgaag acgatgtgat atttacacat ccacttcgta
     5281 tgtgccaatg aatttgtttt ttgtgccata cttaatcacc tttcctttct tataatgaca
     5341 gcttgaacaa ctccattata tcggaaaggt gatatctttg tataacttct ttattgctcc
     5401 acccgcatag caggtggttt tttatttcgc atacttcgta tgctcaactg acttaagtct
     5461 atatgttcaa attttcaatt tgaacaggta aaa
//
LOCUS       DAOMTA010000091         5463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_304_length_5463_cov_2.367419, whole genome shotgun sequence.
ACCESSION   DAOMTA010000091 DAOMTA010000000
VERSION     DAOMTA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_5463_cov_2.367419"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLK68_03760"
     CDS             <1..254
                     /locus_tag="PLK68_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HRM89558.1"
                     /translation="KMVTKLSQEGFLEYQKYGIITLTELGKQWGEYFIYRHQVLNNFL
                     CLLNNSHNELEQVEKIEHFLNKKTIDNLALLTKELHNLQ"
     gene            complement(303..920)
                     /locus_tag="PLK68_03765"
     CDS             complement(303..920)
                     /locus_tag="PLK68_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRM89559.1"
                     /translation="MKVILINGSPNAKGCTYTALEEVSKTLKSEGIETEIIHVGHKDI
                     RGCIGCRQCKTKGKCVFNDIVNDIAPKFKECDGIVIGSPVYFASANGTLVSFIDRLFY
                     SMTADKTMKVGAAVVSCRRGGNSATFDELNKYFTISQMPIASSQYWNMVHGNSPEEVQ
                     QDLEGLQTMRTLGKNMAFLIKSIQLGKKEFGLPEKEEHKFTNFIR"
     gene            1035..1433
                     /locus_tag="PLK68_03770"
     CDS             1035..1433
                     /locus_tag="PLK68_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89560.1"
                     /translation="MCTMKEVCKEIGMNYETLKFYCKEGLVPNVKRDKNNYRNFDEKN
                     IAWLKGIQCLRKCGLGVSEIKIYMNYCLQGPTSIPQRKDMLSQTKEHLLKRIAEINEC
                     IEYIDNKQRFYDDVLEGKCRYTSNLIEINE"
     gene            complement(1521..>1700)
                     /locus_tag="PLK68_03775"
     CDS             complement(1521..>1700)
                     /locus_tag="PLK68_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89561.1"
                     /translation="GPEAVVISSPMTPDMDEVKNVLSSFIDKEFLPEFYYIKEASSYM
                     LSGITKLCVDFIEEE"
     assembly_gap    1701..1710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1711..>2241)
                     /locus_tag="PLK68_03780"
     CDS             complement(<1711..>2241)
                     /locus_tag="PLK68_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89562.1"
                     /translation="VILISREQTRIYYRLNDKCEIIDSNLVIKPTMNIYDLYDIIDTI
                     LLKHSYIDMIGIATPGIVKDEKQLKEPTDGRTIDIKADFEDKYGIDVFVYNNANAAVV
                     GFSLEHPEYDNIIFHSQPFGFGVGGQGIISNGKVIRGKNGIAGEIRYFIRRMQLSDDV
                     HKLAWTQHGAVELVTK"
     assembly_gap    2242..2251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2252..2881)
                     /locus_tag="PLK68_03785"
     CDS             complement(<2252..2881)
                     /locus_tag="PLK68_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89563.1"
                     /translation="MTEKKAVFDFKGWIREHHNTPYEIRLENDDLIKLVTEYGEASIQ
                     FTVIEEYTIVEFSIVSNKDHSVKFYLHFELNDENHAKQLYDEMVETLIGLKEEKTLRV
                     LLSCSAGLTTSMFADNLNSVAGMLGLDYHFDAVSYMSIYEEAEKYDVILIAPQIGYML
                     KRLKESITEKPVLQIPTSVFASYDALAALKFIQSELEIFRQEKSDEQAHE"
     gene            complement(3020..3343)
                     /locus_tag="PLK68_03790"
     CDS             complement(3020..3343)
                     /locus_tag="PLK68_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89564.1"
                     /translation="MAAVLGNKKFLSSYSEIAVPSAEAVIMQYTGYDYVSNEDAPTYA
                     CIGTNDYIADHILMKKRLEHLNKLNIPTEFHCYKGLSHGFGLGTGTIAEGWIDDAIYF
                     WQRQS"
     gene            complement(3345..3890)
                     /locus_tag="PLK68_03795"
     CDS             complement(3345..3890)
                     /locus_tag="PLK68_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89565.1"
                     /translation="MNISENTTIQEVLELPAFKTWGRLLFPINRPIALNMTIKELSSS
                     KIYLWYNYIDVNKTIEIINTLNHDAQNHSIFYNIYSKEELLNDASKRDVGLFFFKGSK
                     DAPFAICNAGGGFVYVGAMHDSFPHALELSKRGYNAFALIYRVEHPYEDLAKAISFIY
                     NNANMLKIDKRHYTLGWVSWC"
     gene            complement(3893..4870)
                     /locus_tag="PLK68_03800"
     CDS             complement(3893..4870)
                     /locus_tag="PLK68_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM89566.1"
                     /translation="MMIKRQLGKTNMYVSPIGLGCMGFSHAYGLAEDKERAIQTIKQA
                     YKMGYNFFDTAECYTGENTDGTISYNEELVGEALKEVREQVVIATKFGVEHKGDHLKL
                     DSSPEKIYASIEGSLKKLQTDYIDLYYQHRIDPKVEPEVVAGVMKELIAAGKIKAWGI
                     SETTEEYLRRAHAVCPVTAIQNRYSMMARWYEDLFVVCKELGITYVAFSPMANGLLSG
                     KFTPDSTFEKGDFRNNMPQYQKEGYEKAKKLLNLLKVLSENKNCTMAQLSIAWMLRKK
                     DFIVPIPGSRKLERLEENFRAGEIEVTIDEIKEIDALLDTIDFDVFGGH"
     gene            complement(4885..5301)
                     /locus_tag="PLK68_03805"
     CDS             complement(4885..5301)
                     /locus_tag="PLK68_03805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM89567.1"
                     /translation="MSILFINGSPNKNGNTVALSEQFLKGKNYQTLHLVDYKIYSYGQ
                     HFTDDQFDEVVLAMSKADTIVIGSPLYWHSMSGAVRNLLDRFYQHVDESLLKGKNMYF
                     IFQGYAPTKQQLEAGEYTMQRFANLYGMNYRGMITE"
BASE COUNT         1830 a         1038 c          656 g         1919 t
ORIGIN      
        1 ctaaaatggt aactaaatta agtcaagaag gctttttaga atatcaaaaa tatggaatta
       61 tcacgttaac tgaattaggt aaacaatggg gtgaatattt tatatatcgt catcaggtat
      121 taaataactt tctctgtcta ctcaataaca gtcataacga actagaacaa gttgagaaaa
      181 ttgaacactt tcttaataaa aaaacaattg ataatttagc cttgcttact aaagaacttc
      241 acaatctaca ataaaaacaa caatatggga acaaatccat taccatactg tcgtcttact
      301 ttttatcgaa taaaattagt gaatttatgc tcctcttttt ccggtaatcc aaattccttt
      361 ttacctaatt gaatactttt aattaaaaaa gccatattct taccaagagt tctcatcgtt
      421 tgtagtcctt caaggtcctg ctgtacttct tctggtgaat taccatgtac catattccaa
      481 tattggctag aggctatcgg catttgagaa atagtaaaat atttatttaa ttcatcaaat
      541 gtagcactat taccccctcg acgacaacta actacagccg ccccaacttt cattgtttta
      601 tcagcagtca tactataaaa taaccgatca ataaatgata ctaaggttcc atttgccgac
      661 gcaaaataaa ctggtgaacc aataacaatg ccatcacatt ctttaaattt tggtgcaata
      721 tcattaacaa tatcattaaa tacacatttc cctttcgtct tacattgacg acaaccgata
      781 catccacgaa tatctttatg accaacatga ataatctcag tttcaatccc ttcactcttt
      841 aaggtttttg atacttcctc taaggctgta tacgtacatc ctttagcatt tggacttcca
      901 ttaattaaaa taactttcat ttttctatcc tccttgattt ctctttttat ttatcttaca
      961 attatattgt aatactcggt agttactacc ggtcaataat tttttaaaat aaatcaaaaa
     1021 tagaggtatt tattatgtgt acgatgaaag aagtatgtaa agaaattggt atgaattatg
     1081 aaacattaaa gttttattgt aaagaaggtc ttgttcctaa tgtaaaacga gataaaaata
     1141 attatcgtaa ttttgatgag aaaaatattg cctggttaaa aggaattcaa tgtttaagaa
     1201 aatgtggatt aggcgtaagt gaaataaaaa tatatatgaa ttattgttta caagggccaa
     1261 cttcaattcc ccaacgtaaa gatatgctta gccaaacaaa agaacatttg ttaaaaagaa
     1321 ttgcagaaat aaatgagtgt attgaatata tcgataataa acaacgcttt tacgatgacg
     1381 tacttgaagg aaaatgcagg tacacaagta atcttattga aataaatgag taaatagctt
     1441 tgataattac cactttaaga aaataagtct ttatcttttg tcactaaaag ataaagactt
     1501 atttctatat tagaaatatc ctactcttct tcaataaaat caacacataa ttttgtaatt
     1561 ccgcttaaca tataagagct ggcttcttta atataataaa attcaggtaa aaattcttta
     1621 tcaataaaag aggatagcac attttttact tcatccatat ccggtgtcat tggtgaactt
     1681 atcactacag cttctggtcc nnnnnnnnnn attttgttac gagctctacg gctccatgct
     1741 gtgtccaagc cagtttatga acatcatcag ataattgcat ccgccggata aaatatctta
     1801 tctcacctgc tataccattt ttccctctaa ttactttacc attagaaatg attccttgtc
     1861 cccctacccc aaatccaaat ggctgtgaat ggaaaataat attatcatac tctggatgtt
     1921 ctaaagaaaa acctacaact gctgcatttg cattattata aacgaaaaca tcaattccat
     1981 atttatcttc aaaatcagct tttatatcaa tagttcttcc atctgttggt tctttcaact
     2041 gcttttcatc tttaacaata cccggtgtcg caattccaat catatcaatg taagaatgtt
     2101 ttaataaaat ggtatcaata atatcgtaga gatcgtatat attcattgta ggtttaatga
     2161 caaggttact atcaattatt tcacatttgt catttaaccg ataataaata cgtgtctgct
     2221 ctctactaat caaaataaca annnnnnnnn nttcatgcgc ttgctcatca cttttttctt
     2281 ggcggaaaat ctcaagctca ctttgaataa actttaaagc agctaaagca tcatacgaag
     2341 caaataccga tgttggtatt tgtaataccg gcttttctgt gatactctct tttaaacgtt
     2401 tgagcatata cccaatttgt ggtgcaatta aaattacatc atacttttct gcttcttcat
     2461 aaatactcat ataagatact gcatcaaaat ggtaatctaa ccctaacatt cctgctacag
     2521 aatttaaatt atcagcaaac atagaagttg taagtcctgc cgaacatgat aataaaacac
     2581 gcaaagtttt ttcttctttt aacccaatca atgtttcaac catctcatca tataattgct
     2641 ttgcatgatt ttcatcattg agttcaaaat gaagatagaa tttaacagaa tgatccttat
     2701 ttgaaacaat actaaattca acgattgtat attcctcaat tactgtaaat tgaatcgatg
     2761 cctcaccgta ttcagttact aatttaataa gatcatcgtt ttctaaacga atctcatatg
     2821 gagtattatg atgctctcga atccaacctt taaaatcaaa aactgccttt ttttcagtca
     2881 ttttatccct cttttctctt tataatttta ttttaacatc cataataatg aacatcaaat
     2941 aacattatct ttatgaccaa ataaaaagac gatacaatat taattaactt aattgtaaag
     3001 tcttttttat tcaacatatt tacgattgtc tttgccaaaa gtaaatggca tcatctatcc
     3061 aaccttcagc tattgttcct gtgcctaagc caaatccatg tgatagaccc ttataacaat
     3121 gaaactcagt tggaatattt aatttattta ggtgctctaa ccgttttttc ataagaatat
     3181 gatctgcaat ataatcgttt gttccaatac atgcataagt tggagcatct tcatttgaaa
     3241 cataatcata ccctgtatac tgcataatga cagcttcggc tgaagggaca gcaatttcac
     3301 tatatgaaga taaaaacttt ttatttccca atactgctgc cattctagca ccagctgacc
     3361 caccccaaag tgtagtgtct tttatctatt ttcagcatat tcgcattatt atatataaac
     3421 gaaatagcct tagctaaatc ttcatacgga tgctcaacac gataaatcaa agcaaaagca
     3481 ttgtacccac gcttacttaa ctctaaagca tgtggaaaac tatcatgcat tgctcctaca
     3541 tatacaaaac caccaccagc attgcatatt gcaaaaggcg catctttact tcctttaaaa
     3601 aagaacaatc ctacatctct ttttgaagca tcatttaata attcttcttt agaataaatg
     3661 ttataaaata ttgaatgatt ttgagcatcg tgattgagtg tatttataat ctcaattgtc
     3721 ttattcacat ctatataatt ataccataaa taaattttag acgaacttaa ttctttaatc
     3781 gtcatattta gagctattgg tctattgatt ggaaatagta accttcccca tgttttaaag
     3841 gctggaagtt ctaatacttc ttgaatagta gtattttcac ttatattcat ttttaatgtc
     3901 cgccgaagac atcaaagtca attgtatcta ataaagcatc aatttctttg atttcatcaa
     3961 ttgtaacttc aatttcccct gctctaaaat tttcttctaa acgttctaat tttctgcttc
     4021 ccgggatagg cacaataaaa tccttttttc ttagcatcca tgctattgaa agttgagcca
     4081 tagtacaatt tttattttct gataatactt ttaacaaatt caataatttt tttgcttttt
     4141 catacccttc tttttgatat tgaggcatat tatttctaaa atcccctttt tcaaaggtag
     4201 aatctggtgt aaatttccct gaaagtaatc cattagccat tggagaaaaa gctacataag
     4261 ttattcctaa ctctttacaa acaacgaata aatcttcata ccatctagcc atcattgaat
     4321 atctattttg aatagctgta actgggcaaa cagcatgtgc tcttcttaaa tattcttctg
     4381 ttgtctccga aattccccat gctttaattt tacccgctgc aatcaattcc ttcataactc
     4441 cagccactac ttcaggttca actttaggat ctatcctatg ttgataatat aaatcaatat
     4501 aatcagtctg taatttcttt aatgatcctt ctatcgatgc atatattttt tctggtgagg
     4561 aatctaattt taaatgatcc cctttatgtt caactccaaa tttagttgca ataacaacct
     4621 gttctcttac ctcctttaat gcttcaccta caagctcttc attataagaa atagttccat
     4681 cagtattctc accagtataa cattcagccg tatcaaaaaa attataaccc attttataag
     4741 cctgcttaat agtttgaatt gctctctctt tatcttcagc taagccataa gcatgagaaa
     4801 atcccataca gcctaatcct atcggactga catacatatt cgttttccct aactgtcttt
     4861 ttatcatcat agcattatcc tttcttattc agtaatcatc ccacgataat tcattccata
     4921 taaatttgca aaacgctgca ttgtatattc ccctgcttcc aattgttgtt tcgttggagc
     4981 ataaccttgg aagataaaat acatgttttt gccctttaat aaactttcat caacatgttg
     5041 ataaaaccgg tctaatagat tacgaacagc tccagacata ctatgccaat aaagcggtga
     5101 gccaattaca attgtatcag ctttactcat cgctaaaaca acttcatcaa attgatcatc
     5161 tgtaaaatgc tgaccataac tataaatctt ataatctact aaatgcaatg tctgataatt
     5221 tttacccttt agaaattgct cacttaaagc aaccgtatta ccatttttat taggacttcc
     5281 gttaataaat aatatactca tttttttcac tcctttaata atctattttt tattcatgga
     5341 ttttcctatg aacaaccatc cttacaaaat caggttcaaa gtgtttagcc cgtgaaccac
     5401 tatatcccat atctaaggat gaaatttttt tcatctcatc attattcaac tcaaaatcaa
     5461 ata
//
LOCUS       DAOMTA010000092         5442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_307_length_5442_cov_2.286430, whole genome shotgun sequence.
ACCESSION   DAOMTA010000092 DAOMTA010000000
VERSION     DAOMTA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_5442_cov_2.286430"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..2069
                     /gene="hcp"
                     /locus_tag="PLK68_03810"
                     /gene_synonym="priS"
     CDS             231..2069
                     /gene="hcp"
                     /locus_tag="PLK68_03810"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRM89568.1"
                     /translation="MMENKMFCYQCQETVGNQGCSQVGVCGKTPETAGLQDLLIDVTK
                     GLAEVINEVRCKGKEINNVYDDQVSENLFITITNANFDDQMIIEAVRKTLTLKKELLL
                     QLDDQAVLSNRALWMEMEIEAIEKRMQMVGVLEAADEDIRSLKQLITYGLKGMAAYNK
                     HAHALGFRKDEIDRFIETTLVKIETSTMSLNDYVALTMETGKYGVQVMELLDQANTST
                     YGNPEITEVNIGVRNNPGILVSGHDLKDLEMLLEQTKDTGIDVYTHSEMLPAHYYPFF
                     KKYPNFVGNYGNAWWKQREEFKAFNGPVLLTTNCLVPPLASYQERVYTTGAVGFEGCV
                     HIDKDEHGYKDFSQIIEHAKKCLAPTQIETGKIVGGFAHHQVLALADQIVTAVKNGDI
                     KKFVVMAGCDGRHPTRQYYTDFAQSLPTDSVILTAGCAKYRYNKLDLGTINGIPRVLD
                     AGQCNDSYSLAMIALKLKEVFALEDINELPVIYNIAWYEQKAVIVLLALLSLGVKNIH
                     LGPTLPAFLSPNIVDFLVDNFQISGIQSVQEDLNLFFPQTKKEDKFHRDMLVGSIIGM
                     DPQAAQILSDSGMGCLGCPASQSETLADACLVHGLDVEEILKQLNQ"
     gene            2080..2652
                     /locus_tag="PLK68_03815"
     CDS             2080..2652
                     /locus_tag="PLK68_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89569.1"
                     /translation="MSAFLGPVHYWLYRKIQYQEQLNQKILKRICPQLNEIVAQECGT
                     IQDGSLEEIIDHQAIHQWLSMELMIVEKRFAFIVEHIEKSDFEEVREVLFEAGKEISI
                     NENYHNCIELFKVINNYLIDGMPCDKGIKIMSQEENQIIYEYNEMVHQYLDFEIFQKY
                     RKAWLDGVLSDSHIVFSRLNYNTYMLKMEE"
     gene            2654..3142
                     /locus_tag="PLK68_03820"
     CDS             2654..3142
                     /locus_tag="PLK68_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRM89570.1"
                     /translation="MTNNIIKDLEADHQKILAFVNELEQRLIEFMEANIFDYEQMKRD
                     ILFIREFADKEHHQREEKILFRYMIEYLGKVAENLVRHGMIVEHDLARLYVKQWDEAL
                     QRYHRNHLLIDKLTIISNGHAYCMLLRRHIEKEDTVVYPFAKKHLSEEIFAEMIKENQ
                     NY"
     gene            3267..4583
                     /locus_tag="PLK68_03825"
     CDS             3267..4583
                     /locus_tag="PLK68_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRM89571.1"
                     /translation="MIKKHKPFIAAILIFMIMSILSFFILYGVINDKVAAAKEKIHYF
                     TEAQVSQLDRVLSSYIQTAETLKLLIVDSNGNINDFDRVAHQLYNDDNTFRSIQLAPQ
                     GDVQYVYPLEGNEGAFGNIFEDPERATEAKKARDTGETTLAGPFELYQSGKGIVVRQP
                     IYLEENGENNFWGFAIVVLNVPEIFDSVHLDSFENMGYEYQLWRIDPDNNKKQIILKS
                     DHELLDDTINLSFEVPGNTWTLSVSPINGWVRSSDLMPVIILAICLSLLIPLLVYTLL
                     KINEQRRRMIDISNRDYLTTLYNGRKMNFVLNDLIARQTSFIFVYLDVDKFKVVNDTY
                     GHLAGDVLLKEIALRIMRYLSREDYAFRVGGDEFVIIIKNNSSTKKTLQIIAEQITEK
                     INLDGFEYYPEVSMGYAVYPNDGKSLEEVIKCADSRMYEIKKHKNI"
     gene            complement(4694..>5442)
                     /locus_tag="PLK68_03830"
     CDS             complement(4694..>5442)
                     /locus_tag="PLK68_03830"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF009022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRM89572.1"
                     /translation="SKIEKELTKMKLIIEENEQKMSESAMFILLGAMMQDKRVNISLT
                     SGRSPKTMYDMMIPYVKNQAKFKDIEYYLFDENPYIDEPYGPNWKDMQELFFKAANIP
                     DERIHIMTSNDWQDYDNKIRNAGGIDVMVIGLGYDGHFCGNCPRCTPFDSYTYCIDFK
                     KKQAVNPDYGDRPRQPHTLTMGPKSLMRVKHLVMIVNGKEKAEIFKRFLDEPINQDVP
                     ATILKLHPNFTVICDQAAASLIDPQQYSNL"
BASE COUNT         1906 a          685 c         1017 g         1834 t
ORIGIN      
        1 tatatgcatc aatattattg tgatcgcaaa aagatatcat gtttaggttt atagaatgag
       61 acatatttaa aatatcaaat acagaatata caccatcaga attattagta tgaacatgta
      121 aatcaatcat ttttgttata tctcctttat tttataattt tatcatatat attttttttg
      181 tcaattgtat ttaaaacatt aaaaaatata gagattataa aggagaaatt atgatggaaa
      241 ataaaatgtt ttgttatcag tgtcaagaga cagtaggaaa tcagggatgt agtcaagttg
      301 gtgtttgtgg aaagacccca gaaacggcag ggctgcaaga tttattaatt gatgttacga
      361 aaggcttagc tgaggtaatt aatgaggtac gttgtaaagg taaagaaatc aataatgtat
      421 atgatgatca agtgagtgaa aatctattta ttacaattac taatgccaac tttgatgatc
      481 aaatgattat tgaagctgtt agaaaaacac ttactttaaa aaaagagctg cttttacagt
      541 tagatgatca agctgtactt tcaaacagag ctctttggat ggagatggag atagaagcaa
      601 ttgagaaaag aatgcagatg gttggagttt tagaagcagc agatgaagat attagaagtt
      661 taaaacagct gattacttat ggtttaaaag gaatggcagc atataataaa catgctcatg
      721 cattagggtt tcgaaaagat gagattgatc gttttattga aactactttg gttaagattg
      781 aaacttcaac gatgtcttta aatgattatg tagcattaac gatggaaact ggaaaatatg
      841 gtgttcaggt aatggaatta ttagatcaag ctaatactag cacttatgga aatccagaaa
      901 taacagaagt taatattgga gttcgaaata acccgggaat tttagtttct ggtcatgatc
      961 ttaaagattt agaaatgcta ttagaacaga ctaaagatac gggaatagat gtttatactc
     1021 atagtgagat gttacctgct cattattatc cattttttaa aaaatatcca aattttgttg
     1081 gaaactatgg taatgcctgg tggaaacaaa gagaagagtt taaggctttt aatggaccgg
     1141 tcttattaac aacaaattgt ttagtaccgc cacttgctag ttatcaagaa cgggtttata
     1201 caactggagc agttggtttt gagggatgtg ttcatattga taaagatgaa cacggctata
     1261 aggatttttc acaaatcatt gagcatgcta aaaaatgctt agctcctact caaattgaaa
     1321 ctggtaagat tgttggtggt tttgctcatc atcaagtact tgcgttggct gaccaaatcg
     1381 taacagctgt taaaaatggg gatattaaaa agtttgttgt aatggcggga tgtgatgggc
     1441 gtcatccaac ccgtcaatat tatactgatt ttgctcaaag cttaccgaca gatagtgtta
     1501 tcttaacagc tggttgtgct aaatatcgtt ataataaact agatttagga acaattaatg
     1561 gtattcctcg agtattagat gctggtcaat gtaatgattc ttattcacta gcgatgattg
     1621 ctttaaaact taaagaggta tttgcattag aagatattaa tgagttacca gttatttata
     1681 atattgcctg gtatgaacaa aaggctgtaa ttgttttatt ggcattgtta tcattaggag
     1741 ttaaaaatat tcatttagga ccaactttac cagccttttt atcacctaat attgttgatt
     1801 ttcttgtaga taattttcaa atttctggta ttcaaagtgt tcaagaagat ttaaatttat
     1861 tttttcctca gacaaaaaaa gaagataagt ttcatcgcga tatgctagtt ggaagtatta
     1921 ttggtatgga tccacaagca gcacaaattt taagcgatag tggaatgggg tgtcttggtt
     1981 gtccggcatc gcaatctgaa actttagctg atgcttgtct agtccatggt ttagatgttg
     2041 aagaaatttt aaaacaatta aatcagtagg aggaatttaa tgagtgcgtt tttagggcca
     2101 gtacattatt ggttatatag aaaaattcaa tatcaagaac aattaaatca aaagattctt
     2161 aaaagaattt gtccccagct taatgagatt gttgctcagg aatgtggcac aattcaagat
     2221 ggaagtctag aggaaattat tgatcatcaa gcaattcatc aatggctttc tatggaattg
     2281 atgatagtag aaaaacgatt tgcatttatt gttgaacata tagaaaaaag tgattttgag
     2341 gaagttaggg aagtgttgtt cgaggctggc aaagaaattt caatcaatga gaactatcat
     2401 aactgcatag aattattcaa agtaattaat aattacctta tcgatggaat gccttgtgat
     2461 aaaggtatta aaataatgag ccaagaggaa aatcaaatta tttatgaata taatgaaatg
     2521 gttcatcaat atttagattt tgaaatattt caaaaatatc gcaaagcttg gcttgatggg
     2581 gtactttcag atagccatat agtattttca agacttaatt ataatactta tatgttaaag
     2641 atggaggaat aaaatgacaa acaatataat taaagattta gaagctgatc atcaaaaaat
     2701 attagcattt gttaatgaat tagagcaacg tttaatagaa tttatggaag caaatatttt
     2761 tgattatgaa caaatgaaaa gagatatttt atttattaga gagtttgctg ataaagaaca
     2821 tcatcagcgt gaagaaaaga ttttatttag atatatgata gagtatttag gtaaagttgc
     2881 tgaaaatttg gttcgacatg gtatgattgt tgaacatgat cttgcaagat tgtatgttaa
     2941 acagtgggat gaagcacttc aacgttatca tcgcaatcat ttgttaattg ataaactaac
     3001 aataatttca aatggacatg cttattgtat gttactaaga aggcatattg aaaaagagga
     3061 tacagtagtt tatccgtttg ctaaaaaaca tttatcagaa gaaatctttg ctgaaatgat
     3121 caaagaaaat caaaattatt aagtataata taagtgaaaa gcagctattt ttgtaatagt
     3181 tgttttttta ttaaaattac ttaaattgag gagtgttgca aatagtgata taattatgtt
     3241 ctatagttat gatagggggt agagtgatga taaagaaaca taagccattt attgctgcaa
     3301 ttttaatatt tatgataatg agcattctat cattttttat cttgtacggt gtcattaatg
     3361 ataaggtagc agcagccaaa gagaaaattc attattttac agaggctcaa gttagtcagc
     3421 tagatcgggt cctgtcttcg tatattcaaa ctgctgaaac gttgaagctg ctaattgtag
     3481 atagtaatgg taatattaat gattttgatc gagttgcaca tcaactatat aatgatgata
     3541 atacatttag aagtattcaa ttagctccgc agggagacgt tcaatatgtt tatcctcttg
     3601 aaggtaatga aggagctttt ggaaatatct tcgaagatcc agaacgtgca acggaagcta
     3661 aaaaagctcg tgatactggg gaaactacct tggctggacc attcgaactc tatcaatcag
     3721 gaaagggaat tgttgttcga caaccaattt atttagaaga aaatggagaa aataattttt
     3781 ggggatttgc aattgttgtt ttaaatgtac cagaaatttt tgattcagta catctagatt
     3841 catttgaaaa tatgggatat gaatatcagt tatggcgtat tgatccagat aataataaaa
     3901 aacagattat tttgaaatcc gatcatgaac tattagatga cacaataaat ttaagttttg
     3961 aagttccagg aaatacttgg actttgagtg tttcaccaat taacggatgg gttcgaagta
     4021 gtgatttgat gccagttatt atattagcta tctgtttgtc attgttgata ccgttattag
     4081 tttatacatt actaaaaatt aacgaacagc gccgaagaat gatcgatatt tctaatcgtg
     4141 attatctaac aacgctttat aatggtcgga aaatgaattt tgttttaaac gacttaattg
     4201 ctcgtcaaac ttcgtttatt tttgtttatt tagatgtaga taaatttaaa gttgtaaatg
     4261 acacatatgg tcatttagca ggagatgtgt tattaaaaga aatagcttta cggatcatga
     4321 ggtacttatc acgagaggat tatgcttttc gagtaggtgg tgacgaattt gtcatcatta
     4381 ttaaaaataa ttcttccacc aagaagaccc tccaaataat tgctgagcaa ataacagaaa
     4441 agataaattt agatggcttt gaatattatc cggaagttag catgggatat gctgtttatc
     4501 ctaatgatgg taaaagttta gaagaagtta ttaaatgtgc tgatagtcgg atgtatgaaa
     4561 tcaaaaaaca taagaatata taatataaaa cacagtgatt gttaaatgat atgattcctc
     4621 ataagtagtt tttggtcatt tgccctatct acttgataga gagaatatca aaaatagatc
     4681 actgtgtttt ttgttataaa ttagaatatt gttgtggatc aatcaaacta gcagctgctt
     4741 gatcgcaaat aacagtaaag ttagggtgaa gctttaaaat tgttgccggc acatcttgat
     4801 taataggctc atctaagaag cgtttaaaaa tttctgcttt ttctttacca ttaacgatca
     4861 tgactagatg tttgactcgc attagacttt taggacccat tgtcaaagta tgaggttgtc
     4921 gaggacggtc tccataatct ggattaacgg cctgcttttt cttgaaatca atacagtaag
     4981 tataactgtc aaaaggtgta caacgaggac aattaccaca aaaatgtcca tcatatccaa
     5041 gaccaatcac cattacatca attcctccag catttctaat tttattatca tagtcttgcc
     5101 agtcatttga agtcataatg tgaatacgct catcaggaat attagcggct ttaaaaaata
     5161 attcttgcat atccttccag tttggtccat aaggttcatc aatatatggg ttttcatcaa
     5221 aaagataata ctcaatatct ttaaatttag cctgattttt tacataagga atcatcatgt
     5281 catacatcgt tttaggagaa cgaccggaag taagtgaaat attgacacgt ttatcttgca
     5341 tcatggcacc taatagaata aacattgcac tttcactcat tttttgttca ttttcttcaa
     5401 ttattagttt cattttagta agctcctttt caatttttga tt
//
LOCUS       DAOMTA010000093         5420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_310_length_5420_cov_2.430755, whole genome shotgun sequence.
ACCESSION   DAOMTA010000093 DAOMTA010000000
VERSION     DAOMTA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_5420_cov_2.430755"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..923
                     /locus_tag="PLK68_03835"
     CDS             267..923
                     /locus_tag="PLK68_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRM89573.1"
                     /translation="MEYFEYSDIEIEYLKSRDPVLKQVIEQIGIIKRAVIPDLFAALV
                     NSIVGQQISTKAHQTIWQRINNSFSPITPANFKSLSAEELQKCGITMKKAHYIKEITR
                     RIISDQLDLERLADLSNEEVIEQLCELPGIGRWTAEMILTFSLQRKDIISYSDLAILR
                     GMRMVYHHRRITPQLFTKYKRRYGPYATIASLYLWAVAAGAIPELKDYAPKSKNNVRI
                     "
     assembly_gap    956..986
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <987..1716
                     /locus_tag="PLK68_03840"
     CDS             <987..1716
                     /locus_tag="PLK68_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89574.1"
                     /translation="KFEERVLQQEFTLNDTDDLIVDYIRKNKANLEHLSIQKVAEDLY
                     LVPNSIMRLCKKLGYKGFAEMKFLVNNEVSENLDIQKLLSKNLYKSMELINYKTLEEV
                     AAKIKNARTCHFIGVGESLYFCEMMVDNLRCYDYRAEYYQTYREIEYRIKHCEEKDIL
                     FVISASGENGRLNNWVEEAKQNGIFVISVTHFNENRLAGLAHIPLYYWGDEQKLNGYN
                     VADRTGMMMLLRELTEVFWRMYCV"
     gene            1863..3749
                     /gene="mngA"
                     /locus_tag="PLK68_03845"
     CDS             1863..3749
                     /gene="mngA"
                     /locus_tag="PLK68_03845"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HRM89575.1"
                     /translation="MELSKLTSEQLITLNSDLSSKDEIIKFLVSKLYQAGKISNEEDF
                     YQAVLERESLTPTGIDNGLAIPHGKDGVVREAAFAVVTLKKPVKDWESVVEGNKVQYV
                     FLLAIPQNDRNSVQMQLLAEMMTKMANHTYTEKLYASKTVKEFYQNLDNGVNSDEIKS
                     FDRSIVAVTACAAGIAHTYMAAEALTKAGQELGVNVYVEKQGANGIEDRHTNEMLKNA
                     SAAIFAVDVAVKEEERFSHLPTIKTKVSAPLKDAKKIIETALVKAEQTARGEYVEHSR
                     HQEAGFLETVKEAVMTGISHVIPLIVAGGMIAAICVIFARTFGFTDLMNTEGSWLYLI
                     KSMGSGLLGTLMVPVLSAYMAYSIGDKPALASGFAAGFCANTVGGGFLLGMLGGIIAG
                     YSIRYLKKWIPAKGTFAGFVSFVIYPVLSCLIVGVLLLVVLGKPVAMINTALVDFLGS
                     MAGTNAALLGAVLGIMVSFDLGGPVNKAAYAFCVAAMAEGVLMPYCAFASIKMVSAFG
                     VTFATKFRKDLFTPEEREVGNSTWLLGLAGITEGAIPFMMADPIRVIISLCTGSAVCG
                     AIVAMFNIGLDVPGAGIFSIFVLTADNIPLAMIVWFGAAVIGAIISAVLLVITRKQKL
                     AKAK"
     gene            3760..>5420
                     /locus_tag="PLK68_03850"
     CDS             3760..>5420
                     /locus_tag="PLK68_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HRM89576.1"
                     /translation="MANQVHIVPHMHWDREWYFSAEESKLLLLNNMEEILEMLENNED
                     YPYYVLDGQTAVLEDYLALKPKNLSRLVNLVKKGKLIIGPWYTQTDEMVVGGESIVRN
                     LLYGHKDSQTFGEPMNIGYLPDSFGQSGQMPMILNGFGITRSIFWRGTSERMGSNKTE
                     FYWTSDDGSKVLTQLLPLGYAIGKYLPTDLDELKKRCDKYMPVLQKGATSEHILIPNG
                     HDQMPLQKNIFDVIEQLKRLYPDKNFFLSNYDQIFKELEKKDNYDILHGELLDGKYMR
                     VHRSIFSSRADIKTMNTVIENKITNLLEPLASIAFKLGFEYFDGMMESIWKEIMKNHA
                     HDSIGCCCSDKVHQEISSRFTIANEKVERMIDFYKRKITDAMPKDIALDKLTVFNLLP
                     YPRNRVVRSEVITRMKSFKIVTSEGHEIDYQVINKEIIDAGLIDRQIVHYGNYDPFIK
                     YTIEFKDNFPAMGYQAYLIQEAVELKEILLDKVDFLENEYYQIKINSNGTLKIYDLVN
                     DHTYDSVLLIEDGSDEGDGYDYSPLLEDYIITSKDAKADYQINRS"
BASE COUNT         1857 a          684 c         1117 g         1731 t
ORIGIN      
        1 ttactagagt atttttatcg ttgtgcttta ttagataatt atgaactaac aatttataat
       61 cataaggata aaagcagtaa tataaactat ttgattacat aaggaaacat acttgattta
      121 tataatattt catttattta caaagtttag tacttcaagt atacttgaaa ttaataaagc
      181 taatcaatat agtcaaaaaa gttatttgat tgttttaaaa tgatggtatt gtaactatgc
      241 gataagatta taggttggag tatataatgg aatattttga atatagcgat attgaaattg
      301 aatatttgaa aagtagggat cctgttttaa aacaagtaat cgagcagatt ggtataatta
      361 aacgtgcggt aatacctgat ttatttgcag ctttagttaa ttcgattgtt ggtcaacaaa
      421 tttctactaa agcccatcaa acaatttggc agagaatcaa caattctttt agtccgatta
      481 ccccagcaaa ttttaaatca ctttcagctg aggaattaca aaaatgtggc attacgatga
      541 aaaaagcaca ttatatcaaa gaaataacga gacggatcat tagtgatcaa ttggatttgg
      601 agcgattagc tgatctatca aatgaagagg ttatagaaca gctttgtgag ttacctggaa
      661 tcgggagatg gacagcagag atgattttaa cattttcatt acaacgtaaa gatataatta
      721 gttatagcga tttagcaatt ttacggggaa tgcgcatggt ctatcatcat cggcgaatta
      781 cacctcaact attcactaaa tataaacgtc gctatggacc atatgcaaca attgccagtt
      841 tgtatttatg ggcagttgct gcgggagcaa ttccagaatt gaaagattat gcaccgaaat
      901 caaaaaataa tgttagaata taagttatat tgagagagtt gatatttatc ttgtcnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnngaaa tttgaagaac gggtcttaca acaagagttt
     1021 acactaaacg atacagatga tctaattgtt gattatattc gtaaaaataa agcaaatttg
     1081 gaacatttat caattcaaaa ggtagccgaa gatctttatc ttgttcccaa ctcaatcatg
     1141 cgtctttgta aaaagttagg gtataaaggc tttgcagaga tgaaattttt agttaataac
     1201 gaagtatcag aaaatttaga tattcaaaaa ttattatcaa agaatttgta taaatcaatg
     1261 gaactaatta attataaaac gttagaagaa gttgccgcta agatcaagaa tgcccgaaca
     1321 tgtcacttta ttggtgtcgg tgagtcatta tatttttgtg aaatgatggt cgataatttg
     1381 cgatgttatg attatcgagc tgaatattat caaacttatc gtgaaattga atatcgaatc
     1441 aagcattgtg aagaaaaaga tattttgttt gtaattagtg caagtggtga gaatggtcgt
     1501 ttgaataatt gggtagaaga agcaaaacag aatggaattt ttgttatttc ggttacccat
     1561 tttaatgaaa atcgattggc tggtttagca catattccat tatattattg gggtgatgaa
     1621 caaaaattga atggctataa tgttgcagat cgaacaggga tgatgatgct gcttcgagag
     1681 ttgacagagg tattttggag gatgtactgt gtataaatac acggtacatc cattttttat
     1741 ttaaaaataa gtgtatgatt ttccaaattg aaaagggtta caaaataata tgctatgata
     1801 tttttgtaat cagggaaaga catgtcggtg attaataaaa ttaaaagaag agggcagaag
     1861 aaatggaatt atctaaatta acaagtgagc agcttattac tttaaatagt gatttgtcat
     1921 caaaagatga aataattaaa ttcttagttt ctaaattata tcaggcagga aaaattagta
     1981 atgaggaaga tttttatcaa gcagtattag aacgtgaaag cttaacacca acagggattg
     2041 ataatggtct ggccattcct catggaaaag atggagtagt tagagaagca gcttttgcgg
     2101 ttgtaacttt aaagaaacct gtgaaggatt gggaaagtgt tgttgaaggt aacaaagtac
     2161 aatatgtttt tttacttgca attccccaaa atgataggaa ttcggttcaa atgcaattat
     2221 tggcagaaat gatgacgaag atggctaatc atacatatac agaaaaatta tatgcttcta
     2281 aaacagtaaa agaattttat caaaatctgg ataatggggt taatagtgat gaaataaaat
     2341 cctttgatcg ctcaatcgtt gcggttactg cgtgtgccgc tggtattgcc catacttaca
     2401 tggcagcaga agcattaaca aaagctgggc aagagttggg agtaaatgta tatgtagaaa
     2461 agcagggggc taatggaatt gaagatcgtc atactaatga gatgttaaaa aatgcaagtg
     2521 cagctatttt tgctgtcgat gtagcggtta aagaggagga acgttttagt catttaccaa
     2581 cgattaaaac aaaagtatct gcaccattaa aggatgctaa aaagattatc gaaacagcgt
     2641 tagttaaagc agaacaaact gctcgtggtg agtatgtaga acattcacga catcaagaag
     2701 ccggattttt agaaacagtt aaggaagcgg ttatgacagg aattagtcat gttattccat
     2761 tgatcgtggc tggcggaatg attgcagcaa tttgtgtaat ctttgcaaga acctttggtt
     2821 ttacagattt gatgaatacc gaaggaagtt ggctttattt aatcaaatca atgggaagtg
     2881 gattgttagg aaccttgatg gtaccggtat tatcagctta tatggcttat tcaatcgggg
     2941 ataaaccagc tttagcttca gggtttgcgg ctggattttg tgctaatacc gttggcggtg
     3001 gattcttact cgggatgctt ggtgggatca ttgctgggta ttcaatacgc tatttaaaga
     3061 aatggattcc agctaaagga acatttgccg gttttgtgag ttttgtaatt tatcctgtat
     3121 tgtcttgttt gattgttgga gtcttattgt tagtcgtatt aggtaaacca gttgcaatga
     3181 tcaatacagc attagttgac tttttaggat caatggcagg aacaaatgct gctttactag
     3241 gggctgtttt aggaatcatg gtttcttttg atttaggtgg accagtaaat aaagctgcat
     3301 atgctttctg tgttgctgca atggcggaag gagttttaat gccatattgt gcttttgctt
     3361 caattaaaat ggttagtgca tttggggtga catttgcgac aaagtttaga aaagatttat
     3421 ttacaccaga agaacgtgaa gtaggaaatt caacctggct attaggttta gcaggaatca
     3481 cagaaggggc aattccgttt atgatggcag atccaattcg tgtaattata tccctatgta
     3541 ctggttctgc agtttgtgga gcgattgtag cgatgtttaa tattggtctt gatgtacctg
     3601 gggcaggaat attctctatt ttcgtattaa ctgctgataa tattccatta gcgatgatcg
     3661 tctggtttgg agcggcagta attggagcga ttatttcagc ggtgctactt gtaattactc
     3721 gaaaacagaa attagctaag gcaaaatagg aggaatagca tggcaaatca agtacacatt
     3781 gtcccccata tgcattggga tcgtgagtgg tatttttcag ccgaagaatc aaagttatta
     3841 ttactaaaca atatggaaga aatattagaa atgttagaaa ataatgagga ttatccttat
     3901 tatgttctcg atggacaaac agctgtactt gaggattatc tagcattaaa accaaaaaat
     3961 ttatcacgac tagttaattt agtgaaaaaa ggtaaattaa ttattgggcc ttggtacact
     4021 caaactgatg agatggttgt aggtggagag tcgattgttc gtaatttatt atacggtcat
     4081 aaagatagtc agacttttgg agaacctatg aatattggtt atttaccaga ttcatttggt
     4141 cagtcaggcc aaatgccaat gattttaaac ggttttggaa ttacaagaag catattctgg
     4201 cggggaacga gtgagcgaat gggttcaaat aagacagagt tttattggac tagtgacgat
     4261 ggttcaaaag tcttaactca actattacca cttggttatg caattggaaa atatttaccc
     4321 acagatcttg atgaattaaa aaaacgttgt gataaatata tgccagtgtt acaaaaagga
     4381 gcaactagtg aacatatatt aattcctaat ggtcacgacc aaatgccatt acaaaaaaat
     4441 atatttgatg ttattgagca gttaaaaaga ctttatccag ataaaaactt ctttttaagt
     4501 aactatgatc agatatttaa agaattagag aaaaaagata actatgatat ccttcatggt
     4561 gaactgctgg atggtaaata tatgcgtgta catcgttcga ttttttcatc acgggcagat
     4621 atcaaaacaa tgaataccgt aattgaaaat aaaattacca atctgctaga gccattggct
     4681 tccattgctt ttaaattagg gtttgagtat tttgatggga tgatggaatc gatatggaaa
     4741 gagataatga aaaatcatgc ccatgattca attggatgtt gttgtagtga taaagtacat
     4801 caagaaataa gcagccgttt tacgattgct aatgagaaag tagagcgaat gattgacttt
     4861 tataaacgaa agattactga tgcaatgcct aaagatattg cacttgataa actaacggtc
     4921 tttaatttac tcccttatcc aagaaatcga gtggttcgca gtgaagttat cacgagaatg
     4981 aaaagtttta aaattgttac tagtgagggt catgaaatag attatcaagt tattaataaa
     5041 gaaatcattg atgctgggtt aattgatcgt cagattgttc attatgggaa ttatgatcca
     5101 tttataaaat atacaattga atttaaagat aattttcctg cgatggggta tcaggcttat
     5161 ttaattcaag aagcagtaga gctaaaagaa attttattag ataaagtgga ctttctggaa
     5221 aatgaatact atcagattaa aattaatagt aatggaactt taaaaatata tgatttagtt
     5281 aatgatcata cttatgattc cgttttattg attgaagatg gtagtgatga gggtgatggt
     5341 tatgattatt caccattatt ggaagattat atcataactt ctaaagatgc taaggcagat
     5401 tatcaaatca atagatccag
//
LOCUS       DAOMTA010000094         5388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_315_length_5388_cov_2.140446, whole genome shotgun sequence.
ACCESSION   DAOMTA010000094 DAOMTA010000000
VERSION     DAOMTA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_315_length_5388_cov_2.140446"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PLK68_03855"
     CDS             complement(<1..1309)
                     /locus_tag="PLK68_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE family protein"
                     /protein_id="HRM89577.1"
                     /translation="MIKSLSKIRKQNKDKFKVPKSAQDVVDAEVIYKDGIFKINNRYT
                     KTYRFKDINYSISSKEEKVGLFLDYSDLLNSLDSSTMTKITINNRKIDLSEFKKNVLI
                     PFQYDDLDEYRQEYNEMLMDKISSTDEIVQEKYITITAFKNSIEEARAFFQRTSVELS
                     SHLGKLGSNCEELNAMERLKILHDFYRNGEEENFYFDMNESARKGHHFKDSICPRSPV
                     FKHKYFKLGDKYGRVLYLSEYARYIKDSFVAELCGLNKNMMYSMDLLTVPTDEAVKEV
                     ETKLLGVETNITNWQRRQNANNNFSAVIPYDMEQQRKESKEFLDDLTVRDQKMIFANI
                     TVVHLANSKEELDADSEVLMSIARKYMCDLTPIAFSSRQLDGLSTALPIGVNKLDIMR
                     TLLTESASIFIPFRAQEVMDKGGIWYGQNAITNNLIMCNKELLL"
     gene            complement(1266..1667)
                     /locus_tag="PLK68_03860"
     CDS             complement(1266..1667)
                     /locus_tag="PLK68_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HRM89578.1"
                     /translation="MEVKINKEIREYTESLFFGLNMRQCAFSALACIVAVSIYFLVIE
                     QLGMEITSWLCMLGAAPFAALGFIKYQGMNAEQIVMAAWRSFLLSNTQLVSRPINLYY
                     DCMKSYLLEKQKEELKKNDKKSFKNKKTKQR"
     gene            complement(1698..2012)
                     /locus_tag="PLK68_03865"
     CDS             complement(1698..2012)
                     /locus_tag="PLK68_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89579.1"
                     /translation="MSRKNHQMVNGQLLQMDKQYSALKQKQKEKIALWMYEETYAYYL
                     QYHKMPMSHRCEIVIDKVYERIQDAEIWIPYGEVYRYYIKRKTKIIHRIEKKLNQSDQ
                     SE"
     gene            complement(2073..2909)
                     /locus_tag="PLK68_03870"
     CDS             complement(2073..2909)
                     /locus_tag="PLK68_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89580.1"
                     /translation="MKLDGIIDNLTNSLETWNEKLAEIWMILTQSLETFKGGHIWEVI
                     QKINGALMAIGLALLVLFFVVGVVKTCTNFSEVKRPEQALKLFLRFAIARGLVVYGME
                     IMMAIFKIIQGITSTIMATVGIGTATNTVLPQEIIEAVEDCGFLESIPLWAVSLLGSI
                     FITILAFVMILTVYGRFFKLYMYTAISPVPLSCAAGETTQSVTVTFLKSYAGVCLEGA
                     VIILSCIIFSAFAASPPVVDVDASAVSMVWSYITEIIFNMLILVGTVKMSDHVVKDMM
                     GL"
     gene            complement(2909..3481)
                     /locus_tag="PLK68_03875"
                     /pseudo
     CDS             complement(2909..3481)
                     /locus_tag="PLK68_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361089.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
     gene            complement(3485..3736)
                     /locus_tag="PLK68_03880"
     CDS             complement(3485..3736)
                     /locus_tag="PLK68_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HRM89581.1"
                     /translation="MLNLGIMPVHAADDPIAVVNNLSDFIFGLIRAVGMILLGFGVVQ
                     VGLSLKSHDPSQRANGFLTVAGGIIITFAKEILNIIVGG"
     gene            complement(3905..>4925)
                     /locus_tag="PLK68_03885"
     CDS             complement(3905..>4925)
                     /locus_tag="PLK68_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM89582.1"
                     /translation="MVMEMLRAGEVHEDDDYYQSPLDILFFKLERRNPNHIAVKYYKD
                     YHSGSAKTLKSIQITLAARLEKFNLEAVAKLTATDELDFTSLGERKVALFAIIPDNDT
                     SFNFLISMLYTQLFQQLFWLADTVYQGSLPVHVHLLMDEFANVSLPDDFDKILSVMRS
                     REVSVSIILQNMAQLKALFEKQWESIAGNCDEFLYLGGNEKSTHQYVSELLGKSTIDT
                     NTYGKSTGHSGNYSTNYQNTGRELMTPDEVRMLDNNLAILFIRGERPIIDRKYDITKH
                     PHIDKTPDGKGKEYHHGLVTKSVATISFSKDISFAEELDTGESYMLLSDEEIEEIQNR
                     QSNEK"
     assembly_gap    4926..4935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4936..>5388)
                     /locus_tag="PLK68_03890"
     CDS             complement(<4936..>5388)
                     /locus_tag="PLK68_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM89583.1"
                     /translation="IYASSQMIDDKIFTQNVRISYDSRKHRRNLLSLVVGGSGAGKTR
                     FYAKPNLLQANTSFVVLDPKGELTRDTANILEELGYEIRVLDLINMDRSYCYNPFAYL
                     KTDNDVQKLVTNLFKATTPKGSQSQDPFWDTAASMLLLSLIFYLRYEAP"
BASE COUNT         1675 a         1058 c          812 g         1833 t
ORIGIN      
        1 ttaacagtaa ttctttatta cacatgatta aattattagt aatggcattc tgtccatacc
       61 atatcccacc tttatccatg acctcctgag cacgaaaagg aataaagatc gatgcacttt
      121 cggttaaaag agtacgcata atatctaact tattgactcc aataggtaat gctgttgata
      181 agccatctaa ctgtcggctt gaaaaagcaa ttggagttaa atcacacata tacttacggg
      241 caatgctcat caatacttca ctatcggcat ctagttcctc tttagagttg gcaagatgaa
      301 ctactgtaat attggcaaag atcatctttt ggtcacgaac ggttaagtca tctaaaaatt
      361 ccttactttc ttttcgctgt tgttccatat cataaggaat gactgcacta aagttgttat
      421 tggcattttg tcgtctttgc cagttagtga tattggtttc aacacctaat aactttgttt
      481 ccacttcttt aacagcctca tcagttggta ctgttaataa gtccattgaa tacatcatat
      541 ttttgttaag tccgcataat tcagctacaa aactatcctt gatataacgg gcatactcac
      601 ttaaataaag cactcgtcca tacttgtcac ctaatttaaa gtatttatgc ttaaataccg
      661 gacttctagg gcaaatggaa tccttgaagt gatgtccttt tctagccgat tcattcatat
      721 caaaatagaa gttttcttct tcaccattgc gatagaagtc atgaagtatc tttaatcgtt
      781 ccattgcatt taattcttca cagttagaac ctaatttccc taaatgagaa gatagttcaa
      841 cgcttgttct ttgaaagaat gctcgtgctt cttcaatcga atttttgaaa gcagtaatcg
      901 taatatactt ttcctgtacg atctcatcag tggaagaaat cttatccatc agcatttcat
      961 tgtactcttg acgatattca tctaagtcat catattgaaa aggaataagc acattctttt
     1021 taaactcact caaatcaatc ttacgattgt tgattgtaat ctttgtcatt gtagaactgt
     1081 ctaatgaatt gagtaaatca ctataatcca agaacaatcc tactttttct tctttcgatg
     1141 aaatggaata attgatgtct ttaaagcgat aggtttttgt ataacgattg ttgatcttaa
     1201 agataccgtc tttatagatg acttctgcat cgacgacatc ttgtgctgac tttggtactt
     1261 tgaatttatc tttgttttgt tttcttattt ttgaaagact ttttatcatt cttttttagt
     1321 tcctcctttt gtttttctaa taagtaagac ttcatgcaat cgtaataaag attgatcggt
     1381 ctactgacta attgggtatt gcttaaaaga aaagaacgcc atgcagccat aacgatctgt
     1441 tcagcgttca ttccctgata ttttataaat cctaatgctg caaaaggtgc agcccctaac
     1501 atacatagcc agcttgttat ttccattcct agttgttcaa tcactagaaa atagatagat
     1561 acagctacaa tgcaggcaag tgcagaaaat gcacattgtc gcatattgag tccaaagaat
     1621 agactttcgg tatattctcg gatttccttg ttgattttga cttccatttt aacctccaaa
     1681 taatagaaaa gaccagttta ctcggactgg tctgattgat ttaatttctt ttcgattctg
     1741 tgaatgatct ttgtttttct tttgatatag tatctgtaaa cttctccata gggtatccat
     1801 atctcagcat cttggatacg ctcataaacc ttatcaataa caatctcaca tcgatggctc
     1861 atcggcattt tatgatactg taaataatag gcataggttt cttcatacat ccatagagcg
     1921 attttttctt tctgcttttg tttaagagca gaatattgtt tatccatttg aagtaattga
     1981 ccattaacca tttgatgatt ttttcttgac atatgaattt cacctctttt aatcattata
     2041 gaagttatac agaagtgtat caataattat ttttataatc ccatcatatc tttgaccaca
     2101 tgatcactca ttttcacagt tccaactaaa atgagcatat taaaaattat ctcggtaata
     2161 taactccaca ccattgatac tgcacttgca tcaacatcaa ctacgggtgg cgaagcagca
     2221 aatgccgaaa agataataca tgacagtata atcactgcac cctctaaaca tacaccggca
     2281 taggacttca aaaaggtaac agtaacactt tgagttgttt ctccagcagc acatgataat
     2341 ggtactggag atatagctgt atacatatag agtttaaaga atcttccata tacggttaat
     2401 atcatgacaa aggctagtat cgtaataaat atactgccta atagggatac tgcccataag
     2461 ggtatacttt ctaaaaatcc gcaatcttct actgcctcaa tgatttcttg gggaagtacc
     2521 gtattggttg ctgtacctat accaactgtt gccataattg ttgatgtgat accttgaatg
     2581 attttaaaaa ttgccatcat gatctccata ccatatacaa ccaaacctct tgcaatcgca
     2641 aatcgcaaaa ataactttaa tgcttgttca gggcgtttga cttcgctaaa attcgtacaa
     2701 gtcttaacaa caccaacaac aaaaaataaa accaacaacg ctaaaccgat tgccatgaga
     2761 gcaccattga ttttttgtat gacttcccat atatgtccac ctttaaaggt ttctaaagat
     2821 tgggtgagta tcatccaaat ctcagctaat ttttcattcc atgtttctaa agagttcgtt
     2881 agattatcta taattccgtc caattttatc acgctccttt ctttgaggtt aaatctatat
     2941 cattatctac ctcatttccc catgtatccc agccatcagt tttttgtctg gcaaacagtt
     3001 caattctagg aatatctcct actaattgaa cgatacgctc tcttacttca tctggctttt
     3061 tactgtgttc ttgaatatga gaaataacga cttgatgaac acttttagat atacgttttg
     3121 gtttcccttt tgtagcaagt aaacacattt ccgcattggc tcgtgtccaa taccccatac
     3181 cccaaaaaag cgaatcactt ttcttatttt gcttgatcca cacaaaagca actgttttat
     3241 attgaaagcc ccaagcctca atgacttcaa aagcctgtaa aaaggtagga aaagttcccc
     3301 ataaaaacag catacaatct ttatcagcaa tatcttttac tggtagtttt ttaatttcat
     3361 caatcatcat ggtaggataa tgactttcag cacttctgcc taatcctttc ttggaataga
     3421 cataatacct tcaaggtgga tcagcataaa tgatttgata tttcttatca ggattatcca
     3481 tgctttaccc tcctacaata atatttaaga tttctttagc aaatgtgatg ataatccctc
     3541 cggcaacagt taaaaatcca ttggctcttt ggctgggatc atggcttttt aaagataaac
     3601 cgacttgcac aacaccaaaa cctagcaata tcatacctac tgctcgaatc aaaccaaaga
     3661 taaaatcact aaggttattg actactgcga ttggatcatc tgctgcatgt actggcataa
     3721 tacctagatt taacatcagc acaaatactg catataaaaa gaaaattctc cttgtttttt
     3781 tgtttagttt cataaattat atttacctcc atttttcaat caaataaaaa aggtgtagtc
     3841 atatgctgat aggcaaaaat aactacacca tattgagagt gacaactctc cttattttga
     3901 aaacctactt ctcattagat tgtctgtttt gtatttcttc aatttcttca tcacttaaaa
     3961 gcatatagga ttccccagta tctagttcct ctgcaaatga aatatcttta gagaatgaaa
     4021 tagtcgcaac ggattttgtg actaaaccat gatgatattc ttttcctttt ccatctggag
     4081 tcttatcaat atgaggatgt ttggtaatat catactttcg atcaataatc ggacgttcac
     4141 cacgaatgaa taatatcgca agattattgt caagcattct cacttcatca ggtgtcatga
     4201 gttctcgtcc agtattctga taatttgttg aataatttcc actatgccct gtacttttgc
     4261 cataggtatt ggtatctatg gttgatttcc ctaatagttc cgaaacatat tgatgagtgc
     4321 ttttttcatt tcctcctaga tatagaaatt catcacagtt ccctgctata ctttcccact
     4381 gtttttcaaa caatgccttt agctgagcca tgttttgaag aataatagat acggatactt
     4441 ctcgtgaacg cataacagaa agtatcttat caaaatcatc aggtaaagat acattagcaa
     4501 attcgtccat gagcaaatga acatgcacag gcaaagaacc ttgatacact gtatctgcta
     4561 accaaaacaa ctgctgaaac agttgcgtat aaagcatact gataaggaag ttaaaagaag
     4621 tatcgttatc aggaataata gcaaataaag cgacttttct ttcacctaat gaagtgaaat
     4681 caagttcatc agtagcagtt agtttggcaa ctgcttcaag gttaaatttc tctaatcgtg
     4741 ctgctagtgt aatttgaata cttttcaatg tttttgctga accggaatga taatccttat
     4801 aatacttaac tgcaatatga ttcggatttc ttcgttccag tttaaaaaac aatatatcta
     4861 aaggtgattg gtaataatca tcatcttcat gaacttcacc ggcacgcaac atctccataa
     4921 ccatcnnnnn nnnnnggggc ttcataacga agatagaaga tgagtgacaa taacagcata
     4981 cttgcggccg tatcccaaaa tggatcttga ctttgtgatc ctttcggtgt cgttgcctta
     5041 aatagattgg taaccagttt ctgcacatca ttatctgtct taagataagc aaaaggatta
     5101 tagcaataag aacgatccat attgataagg tctaacacac gaatttcata tcctaattct
     5161 tcaagtatat ttgccgtatc tctagtgagt tctcccttag gatcaagcac tacaaatgac
     5221 gtatttgcct gtaataaatt tggtttggcg tagaaccttg tctttccggc acctgaacca
     5281 cccacaacca aagaaagcaa gtttcttcga tgctttctgc tatcataact gatacgtaca
     5341 ttttgtgtaa atatcttatc atcaatcatt tgagaactgg catatatt
//
LOCUS       DAOMTA010000095         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_320_length_5327_cov_2.350341, whole genome shotgun sequence.
ACCESSION   DAOMTA010000095 DAOMTA010000000
VERSION     DAOMTA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_5327_cov_2.350341"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1065
                     /locus_tag="PLK68_03895"
     CDS             169..1065
                     /locus_tag="PLK68_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM89584.1"
                     /translation="MYKIITDGSCDLSTERAQELDVSVVPFYVSTDGINHQKEIVELG
                     IRDFYQFMIDNPKVFPKTSMPSIQDYLDVFGPLAKDGIDIICICITTKFSGSYNSAMN
                     AKNMLTDEFPDVKIEVIDATVNTVLQGMLVEEAVAYKQMGATFEETVAYINKIKITGR
                     IFFTIGGMDYLVHGGRVGKLSGIAAGALGIKPLILLKEGEIFNNGLTRGRKKSKQKIV
                     EQIINYFKEDNLELSKYRFCIGFGYDYDEAVEFKTTFLAALKGFDPSFSAEVPIRQIG
                     ATIGVHTGPHPLGVGLLEKLQK"
     gene            complement(1137..3224)
                     /locus_tag="PLK68_03900"
     CDS             complement(1137..3224)
                     /locus_tag="PLK68_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM89585.1"
                     /translation="MNRKKIIFSILTIVIILLSSVGIYSCYEVVETSNGKSINFSSNQ
                     ILYDIFDNIYALSFQLDNVSKKEGIPKYLTANEEASDEFYRNNILYCREMMSEKKNIK
                     YYAEGNNNSLGNTNDDIKNIRNNKELKEKYQWYLMIHFDENGQFSYDTLGCLVAGNQN
                     YELVWNNYKQTYFQYLQEYDEDSTLHNPVNTTIYFAVPHKIVSNSIDTIAWYSEKNNR
                     TINVENIVPFAAIAVAAVGLFILLAPYETIKEINIFKYPAKIKLEPLVIGITLAFAGI
                     IVVIYGLILDTINGYYITKLSGIGFGESSELILGAMNVLSWAAFLFLCMFSVFYLKSF
                     FKEGIINSIKKNTVCIWLLNVLRKIIYKASRFDLNDDTNKTILKIVLANCLIISIICC
                     FFTFGIFFALVYSAILFIILRKRFDELKHDYEILLDAVKRLSNGDFDVEINQDIGMFN
                     SLGTEFSNIKDGFEKAVSEEVKSQKMKTELISNVSHDLKTPLTSIITYIDLLKDEQLD
                     YEKRKEYLDTLDRNSLRLKNLIDDLFEVSKVNSGDVKLNLVDVDIIALIQQAKFELID
                     KFNEKSLIFKTAFPNEKIILSLDSLKTYRIFENLLMNIGKYALENTRVYIDIDNSDDE
                     VTITFKNISADEIKVSEDELVERFVQGDTSRNTSGSGLGLAIAKSFTELQKGNFKISV
                     DGDLFKASVTFKK"
     gene            complement(3233..3943)
                     /locus_tag="PLK68_03905"
     CDS             complement(3233..3943)
                     /locus_tag="PLK68_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM89586.1"
                     /translation="MNQDNILVVEDEIEIAKAIEIYLKSQGYNVYIANNGKAGLEMVE
                     RYDIHLAIVDIMMPVMDGIEMTMKIRENYDFPIIFLSAKSEDIDKITGLNIGGDDYVT
                     KPFVPMELLARVSSQLRRYHKYLNMIGKIQEDQAANKYVVGGLELDLDTKEVSVDGKA
                     VKVTPIELKILELLMEKPGRVFSSEQIYENVWHEAAINTETVMVHVRNLREKIEINPS
                     NPQYLKVVWGVGYKIEKQ"
     gene            complement(4019..4696)
                     /locus_tag="PLK68_03910"
     CDS             complement(4019..4696)
                     /locus_tag="PLK68_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRM89587.1"
                     /translation="MDKLNEHKKNLSLLITGFIIITILCLVLYHFFENFLNDPIELKE
                     KLTEFGSWGKLILVAAMAIQVIFVFLPGEIIEIAAGFSYGTIEGMAICLLGAIIGTIV
                     IYAIVNRYGIKLVNHFYDTNKFNEISFLKKEKNLEIILFVIFFIPGTPKDILTYLAPF
                     TRLKLSSFIFITSIARIPSVITSTIGGNAISNQNYRFTIFIFVITGIISIFGLLSYKR
                     YIKKKNL"
     gene            4880..5239
                     /locus_tag="PLK68_03915"
     CDS             4880..5239
                     /locus_tag="PLK68_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM89588.1"
                     /translation="MKNTIQDSESLRKQFLASQKVFNALGDETRMYLLLIMLEGPCDG
                     SRVIDLAPKVNLSRPAVSHHLQILKQAGIVKTRKVKTCIYYYLEPQHCEIDKLINLFQ
                     HIKEIMNNVSRCEMEEE"
BASE COUNT         1874 a          788 c          814 g         1851 t
ORIGIN      
        1 ttaatcaaag tgaggctttt ttaaaacagc tggattttca aaatccagtt gtccaagctg
       61 aaatagaacg gagtttatcg ctgatcaata aaagaaaaag taaagtagtt tattaaaagt
      121 taataattag gggttataat tatagtatta tagagaagag gaaaaagaat gtataagatt
      181 ataacagatg gatcttgcga tctgtcaacc gaacgtgcac aagagcttga tgtgtcagta
      241 gtaccttttt atgtatcaac agatgggatt aatcatcaaa aggaaattgt tgaattgggt
      301 atccgtgatt tctatcaatt tatgattgat aaccccaaag tatttcctaa aacgtcaatg
      361 ccgtcgattc aagattattt agatgttttt ggacctttgg ctaaagatgg tattgatatt
      421 atttgtattt gtattacaac aaaattttcg ggatcatata attcagcaat gaatgctaaa
      481 aatatgctta ctgatgaatt tcctgatgtg aaaattgaag ttatcgatgc taccgttaat
      541 acggtattac aaggaatgtt agtagaagaa gcggttgcat acaagcagat gggtgcaacc
      601 tttgaggaga cggttgcata tattaacaaa attaaaatca ctggaaggat cttctttact
      661 attggtggta tggattattt agttcatggt ggaagagttg gaaaattatc aggaattgcg
      721 gctggagctt tgggaattaa acctttgatc ttgcttaaag aaggagaaat atttaataac
      781 ggattaacac gcggacgaaa aaaatctaaa caaaagatag ttgaacaaat tattaattat
      841 tttaaggaag ataatttgga attgagtaaa tatcgttttt gtataggatt tggttatgat
      901 tatgatgagg ctgtagagtt taaaacaaca ttccttgctg ctttgaaagg gtttgatcca
      961 tcatttagtg ctgaggtacc aattcggcaa attggagcaa cgatcggtgt tcatacagga
     1021 ccacatccac taggtgttgg tctacttgaa aaattacaaa agtaaaatga aaattcaatt
     1081 atgataaaaa gacttgtaga ccttatttgt taggatacaa gtctttttgt atgaaattat
     1141 tttttaaaag ttaccgatgc tttgaataaa tcaccatcaa cactaatttt aaaattacct
     1201 ttttgcagtt cagtgaaact tttagcaata gctaaaccga ggccactacc actagtattt
     1261 cttgaagtat caccttgaac aaaacgctca actaattcat cttcgctaac ttttatttca
     1321 tctgctgata tatttttaaa agtgattgta acttcatcat cactattgtc aatatcgata
     1381 taaacacgtg tattttctaa agcgtatttt ccaatattca ttaagagatt ttcaaaaata
     1441 cgatatgttt ttaaactatc taatgataaa ataatttttt catttggaaa agcagtttta
     1501 aaaattaggc ttttttcgtt aaatttatct attaattcaa acttagcttg ctggatcaat
     1561 gcaataatat cgacatcaac aaggttgagt tttacatcac cactattgac tttactaact
     1621 tcaaaaagat catcaattaa gttctttaat ctaagtgaat tacgatctag tgtatcgaga
     1681 tattcttttc ttttttcata atcaagttgt tcgtctttta acaaatcaat ataagtaatg
     1741 atggaggtta atggtgtttt taagtcgtga gagacattag agattaattc agttttcatt
     1801 ttttgtgatt ttacttcctc gctaacagct ttttcaaagc catctttaat attagaaaat
     1861 tcagtaccta aagaattaaa cataccaata tcttgattga tttcaacatc aaaatcaccg
     1921 tttgataaac gtttaacagc atccaataga atttcataat catgtttaag ttcatcaaat
     1981 cgttttctta aaataataaa taaaatggca gaatatacta aggcaaagaa aataccaaag
     2041 gtaaagaaac aacagattat tgatataatt aagcagtttg ctaaaacgat tttaagaatt
     2101 gttttatttg tatcatcatt taagtcaaat ctactggctt tataaattat ctttcttaat
     2161 acattaagta accaaataca aacagtattt tttttaatag aattaataat cccttcttta
     2221 aagaatgatt ttaaataaaa gacagagaac atacatagga ataaaaaagc tgcccatgat
     2281 agtacattca tcgctccaag gatcagttca cttgattcac caaaaccaat ccctgacaat
     2341 ttagtaatgt aatagccgtt tatggtatcg agaattaaac cataaattac aacgattatt
     2401 cctgcaaatg ccaatgtaat cccaattact aatggttcta atttgatttt agcgggatat
     2461 ttaaagatgt tgatttcttt aattgtttca tatggagcta ataagataaa taagccaaca
     2521 gcagctacag caattgcagc aaatggcaca atattttcaa catttatagt tcgattgttt
     2581 ttttcgctat accaggcaat cgtatcaatt gaattgctta cgattttatg gggaacggca
     2641 aagtagatag ttgtatttac tggattatgc aatgtactat cttcatcata ctcttggaga
     2701 tattgaaaat atgtttgttt ataattattc caaactagtt catagttttg attgccagct
     2761 actaaacagc ctaaagtatc atagctaaat tgaccatttt catcaaagtg aatcattaaa
     2821 taccattgat atttttcttt caattcttta ttgttacgaa tgttttttat atcgtcatta
     2881 gtattaccta agctattatt attgccttct gcatagtatt taatattttt cttttcagac
     2941 atcatttcac gacaatataa aatattattc cgataaaatt catcactagc ttcttcgttg
     3001 gcagtaagat attttggaat accttctttt ttgctgacat tgtcaagctg gaagctcaga
     3061 gcatagatat tgtcaaaaat atcataaaga atttgattac ttgaaaagtt aatacttttc
     3121 ccgttactgg tttctacaac ctcatagcag ctatatatac caacactgct aaggagaata
     3181 attacaattg ttaaaattga aaaaattatt ttttttctgt tcattttatc acctactgtt
     3241 tttcaatttt ataaccgaca ccccagacaa cttttaaata ttggggatta ctagggttaa
     3301 tttcgatttt ttctcgcaaa tttcgcacat gaaccatgac tgtttctgtg ttgattgcag
     3361 cttcatgcca aacattttca taaatttgtt cgcttgaaaa aactctcccg ggcttttcca
     3421 ttagtaattc gagaattttt aattcaattg gagtgacttt aacagcttta ccgtcaacac
     3481 taacttcttt ggtatcaaga tcaagttcta gaccacccac aacatattta tttgctgctt
     3541 gatcttcttg aatcttaccg atcatattta aatatttatg atagcgtcgt aattgtgaag
     3601 aaacacgcgc taggagttcc attggaacaa atggtttggt gacataatca tcaccaccaa
     3661 tatttaatcc tgtaatttta tcaatatctt cagatttagc ggataagaaa ataattggga
     3721 aatcataatt ttcccttatt ttcattgtca tttcaattcc atccattact ggcatcataa
     3781 tatctacgat agcaagatgg atatcatatc tttccaccat ctctaaacca gctttcccgt
     3841 tattagcaat atatacatta taaccttgtg attttaagta tatttcaatg gctttggcaa
     3901 tttcaatttc gtcttctacc actaatatat tatcttgatt catcctcata ccctctttca
     3961 taatattaat tataaagcca atcattaaag aatagtagaa atgatttcaa aagcttttct
     4021 aaagattttt tttcttgatg taacgcttat agctaagaag gccaaaaatg gaaataattc
     4081 cagtaattac aaaaataaat attgtaaaac gataattttg atttgagata gcgttgcccc
     4141 ctattgtact tgtaattaca gatgggattc tagcgatact agtaatgaat ataaaacttg
     4201 aaagttttaa acgcgtaaaa ggtgcaaggt atgttaaaat gtctttagga gtgccgggaa
     4261 taaagaagat gacaaagagg ataatttcaa gatttttttc tttttttaaa aatgaaattt
     4321 cattaaattt gttagtatca taaaaatgat taactaactt gatgccatat ctattaacaa
     4381 tagcatagat aacgatagta ccaataatag caccaagtaa acaaattgcc atcccttcaa
     4441 tagttccata actaaatccg gcagcaatct cgattatttc acctggtaaa aaaacaaaaa
     4501 taacttggat tgccatagca gcaacaagaa ttaattttcc ccaactacca aattcagtta
     4561 acttttcttt caattcaata ggatcattta aaaagttttc aaaaaaatga taaagaacta
     4621 gacacagaat tgtaataatt atgaaaccgg ttattaaaag cgaaaggttt tttttatgct
     4681 catttagttt atccatcttt ttcacctcgt tattagtata gacaacgaat ctaaaacata
     4741 actagcaaaa gttgaaaagt gattctaaag aaaacttaaa gataatatct ctatcttgat
     4801 tttctttacc gataaaataa gtaacagtta ttatgctata atacaagtgt ttaaatattt
     4861 gaacagggag gatgaatcga tgaaaaatac aattcaagat agcgaatctt tgagaaaaca
     4921 atttttagcg agtcaaaaag tttttaatgc acttggtgat gagacaagaa tgtatttatt
     4981 attgattatg cttgaaggtc catgtgatgg tagtcgggta attgatttgg ctcccaaagt
     5041 aaatctttct agaccggcgg tatctcatca tttacaaata ttaaagcagg caggaattgt
     5101 taagacgcgg aaagttaaga cttgtattta ttattattta gaaccgcagc attgtgaaat
     5161 tgataaactg attaatttat ttcagcatat taaagagatt atgaataatg tatcaaggtg
     5221 tgaaatggag gaagagtaat ggaactttta gaagcagttc aaaaacggca ttcagtcaga
     5281 atgtatcaag atagagaaat tccagattct ataaaaaaag aattatt
//
LOCUS       DAOMTA010000096         5305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_324_length_5305_cov_2.382476, whole genome shotgun sequence.
ACCESSION   DAOMTA010000096 DAOMTA010000000
VERSION     DAOMTA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_5305_cov_2.382476"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="PLK68_03920"
     CDS             <1..1718
                     /locus_tag="PLK68_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRM89589.1"
                     /translation="TLMKENGIDIYIIPTSDFHQSEYVGEYFRGRKFLSGFTGSAGTL
                     VISLDEARLWTDGRYFIQAEQQLAGSGIILMKMAMPGVPTIKEYLDQNTDKTVGFDGR
                     VMSYQEVARLSNKLITDVDLVDEVWSERPSISHEPAFIYDEEYCGESRASKLARLRSA
                     MGDCQHHIITSLDDIVWLFNIRGNDVDCNPVVLSYALINQDNAILYVQDNVVDVKTEA
                     ILKRDSIIIRAYNDIYEDVKKLTGKVLLDDQIVNYQIFNNLNCEIKTAPNPTQHFKAI
                     KNETEIKATKNAHIKDGVAMTKFMYWLKNNVGKIELDEVTISDKLAAFRKEQNEFFDL
                     SFDTICGYKANAALMHYKAEPGNCAKVTNEGMLLIDSGGQYLDGTIDTTRTFVLGPIS
                     DIERRDFTVALKAMFRLQAAHFLAGTTGPNLDLLARGIVYEYNLDYRCGTGHGVGHFL
                     NVHEGPNGFRPHDRPGFAKMCAFEPGMITTDEPGIYIENSHGVRHENELLCVEVETNE
                     YGQFLKFEPITMVPFDLDGLDLELLSNHEIKQINDYQQLVFDHVAPFLTNEERAWLQA
                     NLLIK"
     gene            complement(1811..2326)
                     /gene="nrdG"
                     /locus_tag="PLK68_03925"
     CDS             complement(1811..2326)
                     /gene="nrdG"
                     /locus_tag="PLK68_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM89590.1"
                     /translation="MEIRLFGTVNDSIVDGPGLRYAIFTQGCFHNCLGCHNPKSHDIN
                     GGYIRDTSEIINEIDENPLLDGITLSGGEPMLQIEPLKELCKAARLRKLNIVIYSGFT
                     FEQIMDDPNKKSLLELCDMLIDGKFELDKKSLALLYRGSSNQRLINIQQSLSQGEVVE
                     YLADENNEIKI"
     gene            complement(2334..4637)
                     /locus_tag="PLK68_03930"
     CDS             complement(2334..4637)
                     /locus_tag="PLK68_03930"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HRM89591.1"
                     /translation="MIENIRKRDGRLVAFELDKIAGAIYKAFEASGGKYELKNAMNIA
                     KTVETKLEKKKSELPTVELVQDTVEEVLIEQGYVRVAKAYILYRANRTRERDMKSRLM
                     KTFEEITFADSKDSDLKRENANVNADAPMGTMLKYGAEAAKQFNEMFVLNPKHARAHI
                     NGDIHIHDMDFLTLTTTCCQIDLIKLFAGGFSTGHGVLREPNSISIYAALACIAIQSN
                     QNDQHGGQAIPNFDYSMAPGVAKSYLKHFYHNLAKAITLLVKLDGEDIATKVKGEMRL
                     KPTLDKNEAFEEQLKETLKANKVDKFVDEIVEFAITSAYEETDRETYQAMEAFIHNLN
                     TMHSRAGAQVPFSSINYGMDTSSEGRMVMRNILLATEAGLGHGETPIFPIQIFRVKEG
                     INYNEGEPNYDLFKLACRTSAKRLFPNFSFVDAPFNKQYYKGTPETEIAYMGCRTRVM
                     GNVYAPDKEISFGRGNLSFTSINLPRLAIRSKGDVNEFFDKLDGMLDLCIEQLLERFE
                     IQCRRKAKNYPFLMEQGVWLDSDELKPDDEVREVLKHGTLTVGFIGLAETLKALIGVH
                     HGESEKAQILGLQIVAHMRKAMDKATEKYNLNFSLIATPAEGLSGRFVKMDKKLFGEL
                     DGITDREYYTNSFHIPVYYPISAFKKIKLEGPYHELTNGGHISYVEMDGDPTKNIAAF
                     EKIVRAMHDNGIGYGAINHPVDRDPVCGYNGIIDDVCPLCGRSEEEHHGFERIRRITG
                     YLVGTLDRFNDGKKAEESDRVKHETKE"
     gene            complement(4791..>5305)
                     /locus_tag="PLK68_03935"
     CDS             complement(4791..>5305)
                     /locus_tag="PLK68_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HRM89592.1"
                     /translation="GYVYDENPPYELLYSNDLSPEDIGKIHLAEEMLEKFWNSGKMPI
                     TMNTVIRTVASPFYFFLDLGNYYLKQQFKMFKFQNDELFSYLDEYLNRQYQDLLIEDY
                     LRLSKIKPKRWWKARLDKSEARETMHYLIEKYDLDQEEVFRYGVVEKVTDGYLLAIYQ
                     NYQVSVKKYS"
BASE COUNT         1723 a          999 c          763 g         1820 t
ORIGIN      
        1 aaacgttaat gaaagaaaat ggaattgata tttatatcat tccaactagt gattttcatc
       61 aaagtgaata tgttggtgaa tattttagag gacgtaaatt cttatcagga tttaccggtt
      121 ctgctggaac attagtaatc agtctagatg aagcacgttt atggactgac gggcgttatt
      181 ttatccaagc agaacaacaa ttagctggaa gtggcattat tttaatgaaa atggctatgc
      241 ctggggttcc aacaatcaaa gaatatcttg atcaaaacac tgataagact gttggttttg
      301 atggacgagt aatgtcatat caagaagttg ctcgtttaag taataaacta attacagatg
      361 ttgacttagt tgatgaagtt tggagtgaac gccctagcat ctctcatgag ccagctttta
      421 tttatgatga agaatattgc ggtgaatcac gtgcttcaaa acttgcacgt ttgcgcagtg
      481 ccatgggaga ttgccaacac catattatta ctagcttaga tgatattgtc tggttattta
      541 acattcgtgg taacgatgta gattgtaatc ccgtcgtact atcttatgct ttgattaatc
      601 aagataatgc tattctatat gttcaagata acgtcgttga tgttaaaact gaagctattt
      661 taaaaaggga tagtattatc atcagagctt ataatgatat ttatgaagat gttaaaaaac
      721 taactggaaa agtcttatta gatgatcaaa ttgttaatta tcaaatcttc aataatctaa
      781 attgtgaaat aaaaacagca cctaatccaa ctcaacattt taaagcgata aaaaatgaaa
      841 ctgaaatcaa agcaaccaaa aatgcccata tcaaagatgg tgtcgctatg actaaattca
      901 tgtattggtt aaaaaacaac gttggaaaaa tcgaattaga tgaagttacc atctctgata
      961 aattagcagc atttagaaaa gaacaaaatg aattttttga tctaagtttt gatactattt
     1021 gcggatacaa agctaacgct gctttgatgc actacaaagc agaacctgga aattgtgcaa
     1081 aagtaactaa cgagggaatg ctattaatcg attctggtgg tcaatattta gatggaacga
     1141 tcgatacaac aagaaccttt gttttaggtc ctatcagtga tattgaaaga cgtgatttta
     1201 ccgtggcttt aaaagcaatg tttagactac aagcagctca tttcctagcg ggaacaactg
     1261 gtccaaacct agatttatta gcacgtggta tagtttatga atacaatctt gattatcgct
     1321 gcggaacagg acatggagtt ggtcacttct taaacgtcca tgaaggacca aatggattca
     1381 gaccccatga tcgtccagga tttgctaaaa tgtgtgcttt tgaaccagga atgatcacaa
     1441 cagatgaacc tggtatttat atcgaaaata gtcatggtgt tcgtcatgaa aatgaattgt
     1501 tatgtgttga agtcgaaact aatgaatatg gtcagttctt aaaatttgaa ccaattacaa
     1561 tggttccatt tgatttagat ggtttagatc ttgaattatt atctaatcat gaaatcaaac
     1621 aaatcaatga ttatcaacaa ttagtatttg atcatgttgc accattttta acaaatgaag
     1681 agagagcttg gttacaagct aacttattaa ttaaataagt atagtctaaa ggcacacttg
     1741 catagtgcct ttttatttgc tttataaact aatcaccaat ctttaaaaca aaaaaggaag
     1801 ctgtgcttcc ttaaatctta atttcattat tctcatcagc caaatattca actacctctc
     1861 cttggcttag agattgttga atattaatca atctttgatt agatgaacca cgataaagta
     1921 aagctaaact ttttttatct aattcaaatt tgccatcgat tagcatatcg caaagttcca
     1981 ataatgattt tttattagga tcatccataa tttgttcgaa tgtaaaacca ctataaataa
     2041 caatatttag ttttctaagt cgagcagctt tacaaagttc ttttaaaggc tcgatttgta
     2101 acattggttc accacctgat aacgtgattc catctaataa aggattttca tcaatttcat
     2161 taattatttc actagtatct ctaatatacc cgccattaat atcatggctt ttaggattgt
     2221 ggcagcctaa acaattatga aaacaacctt gggtaaaaat cgcatatctt aagcccggcc
     2281 catcaacaat agaatcatta accgtaccaa ataatctaat ttccattaat tattcattcc
     2341 ttggtttcgt gttttacacg atcactttct tcagcctttt tcccatcatt aaaacgatct
     2401 aatgttccta ctaaataacc agtgattcgc cggattcgtt caaaaccatg atgttcttcc
     2461 tcgcttcttc cacataacgg gcaaacatca tcaataattc cattataacc acatactgga
     2521 tcacgatcaa ctggatgatt gattgctcca taaccaattc cattatcatg cattgctcta
     2581 acaatttttt cgaatgcagc aatattttta gtcggatccc catccatctc gacataacta
     2641 atatgaccac cattagttaa ttcatgatat ggtccttcta attttatttt cttaaaagca
     2701 ctaattggat aatatacagg aatatggaaa ctattagtat aatattcacg atcagtaatc
     2761 ccgtctaatt ctccaaaaag ttttttatcc attttaacaa aacgacctga aagtccttct
     2821 gccggagtag caatcaaact aaaattcaaa ttatattttt cagttgcttt atccattgcc
     2881 ttgcgcatat gagcaacaat ttgtaatcct aagatctgag ctttttcact ttcaccatga
     2941 tgtaccccaa tcaaagcttt taatgtttcc gctaatccga taaatccgac tgttaaagtt
     3001 ccatgtttta aaacctcacg aacttcatca tcaggtttta attcatcact atcaagccaa
     3061 actccctgct ccattaaaaa aggatagttt ttagccttac gacgacattg aatttcaaaa
     3121 cgttctaaca attgctcaat acataaatct aacattccat caagcttatc aaaaaactca
     3181 tttacatctc ctttagaacg aattgccaat cttggtaaat tgatagaagt aaaacttaaa
     3241 tttccacgcc caaaactaat ttctttgtcc ggagcataaa catttcccat aactcgtgtt
     3301 cgacatccca tataagcaat ttcagtttct ggcgttcctt tataatattg tttattgaaa
     3361 ggagcatcaa caaatgaaaa attagggaat aaacgttttg cagaggtacg acaagctaat
     3421 ttaaaaagat cgtaattagg ctcaccttca ttatagttga ttccttcttt tactctaaat
     3481 atttgaattg gaaaaattgg tgtttcacca tgacctagcc cagcttctgt tgctaataat
     3541 atattacgca ttaccatgcg cccttcactt gaagtatcca taccataatt gattgaacta
     3601 aacggtactt gtgctccagc tcttgaatgc atagtattaa gattgtgaat aaacgcctcc
     3661 atcgcttgat atgtctctcg atcagtttct tcataagcgc ttgtaattgc aaattcaacg
     3721 atttcatcta caaatttatc tactttatta gctttcagtg tttcttttaa ttgttcttca
     3781 aatgcctcat ttttatctaa agtaggtttt aaacgcattt ctccctttac tttagtcgca
     3841 atatcttcac catctaattt tactaataaa gtaattgctt tagctaaatt atgataaaaa
     3901 tgctttaagt atgattttgc aacacctggt gccatactat aatcaaaatt aggaattgcc
     3961 tgacctccat gttgatcatt ttggtttgac tggatagcaa tgcaagctag agcagcatag
     4021 atactaatgc tatttggctc tcttaaaact ccatggccag ttgaaaatcc tcctgcaaat
     4081 aatttaatta aatcaatttg acaacaagtt gtggttaaag ttaaaaaatc catatcatga
     4141 atatgtatat ccccattaat atgagcacgt gcatgtttag gatttaatac aaacatctca
     4201 ttaaactgtt ttgctgcttc agctccatat ttcagcattg tccccatcgg agcatctgca
     4261 ttcacgttag cattttctct ttttagatca ctatctttac tatcagcaaa agtaatctct
     4321 tcaaaagtct tcattagccg tgacttcata tccctttcac gcgtacgatt agcgcgatat
     4381 aaaatatagg ctttagccac tcgtacatat ccttgttcaa tcaatacttc ttcaaccgta
     4441 tcttgaacta attctaccgt tggtaattca ctcttctttt tttctagctt tgtttcaact
     4501 gttttagcaa tattcatggc attttttaat tcatatttac caccacttgc ttcaaatgct
     4561 ttgtatatag ctcctgcaat cttatctaac tcaaatgcga ctaggcgccc atctctctta
     4621 cgaatatttt ctatcattct atatccctcc atgcactatt aagtatatct tatttgtatt
     4681 tttaattcat gttatatact cctttaccca gcccttttta taatcataaa ataaaacgct
     4741 ctcccattac aggaaaacgt tatctaacgt aatataaccg tttatcatta tcaagaatac
     4801 tttttcacac taacctgata attttgataa atcgctaata agtagccatc agttaccttt
     4861 tcaaccaccc catatctaaa gacttcttct tggtctaagt catatttttc tatcaaataa
     4921 tgcattgttt cacgtgcttc gcttttatct aaacgggcct tccaccagcg tttaggtttt
     4981 atttttgata accgtaaata gtcttcaatc aataaatcct gatactgccg atttaaatat
     5041 tcgtctaaat aactaaataa ttcatcattt tgaaatttaa acattttaaa ttgttgtttc
     5101 aaataataat tgcccaggtc taagaaaaaa taaaaaggtg aagcgactgt tcttatcact
     5161 gtattcattg ttattggcat cttgccacta ttccagaatt tctctaacat ttcttctgcc
     5221 aaatgaattt tcccaatatc ttcagggctt aaatcattac tataaagcaa ttcatagggt
     5281 ggattttcat catatacata cccat
//
LOCUS       DAOMTA010000097         5265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_330_length_5265_cov_2.176967, whole genome shotgun sequence.
ACCESSION   DAOMTA010000097 DAOMTA010000000
VERSION     DAOMTA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_330_length_5265_cov_2.176967"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1240)
                     /locus_tag="PLK68_03940"
     CDS             complement(38..1240)
                     /locus_tag="PLK68_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HRM89593.1"
                     /translation="MLVVYLGIVFLIIVLLLMFRRPLYQSIIGGLIMMVILYKIPFND
                     IFIQITHVFTNWSSLQILISLYLITFLQKMLEKRQQIKLAQQDLNGLFHNRRINAGGA
                     PLFIGLLPSAAAMILCGDIVKDATDGYLKPKDQAFVASWFRHIPESTLPTYAAVLLMA
                     NISGVPLSEFMIGMIIPVFALALIGYFSILVKIPSSTNTPKSQNRMKDFIHLFQHLWS
                     LLMILVLILLFHLSIVSAVLIVIALCCMIYRFKINELLPLITKAFEKKMIVNTFLVLV
                     FKEFIAYTGVLNVLPDLLLSLPIPPYLVFVILFFIGGIISGSSGIIALGTPIAFATIQ
                     GGMPLMVLLMCICHGASQLSPTHICLVVVADYFKVSLGDLIRKTIPKTLLFCLIAIIY
                     YNLLILFL"
     gene            complement(2452..2544)
                     /locus_tag="PLK68_03945"
     CDS             complement(2452..2544)
                     /locus_tag="PLK68_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM89594.1"
                     /translation="MYAPVTGAAIPIENVPDKVFASKMMGREKV"
     gene            complement(2807..4204)
                     /locus_tag="PLK68_03950"
     CDS             complement(2807..4204)
                     /locus_tag="PLK68_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89595.1"
                     /translation="MNMKESFLFGGALAANQCEGGYNLDGKGLSIADVVRGSQQGIPR
                     QVDEKIHEGVYYPSHEAIDFYHRYKEDIALFAQMGFKCLRMSISWARIFPNGDDEIPN
                     ECGLQFYDKVFDELLKYNIQPIVTLSHFETPLHLVEAYGSWRNPKLIDFYVKYANVVF
                     KRYKDKVIYWMTFNEINETMNKKDPFLQAGLKFKEDENDKKVKVIASHNMFIASAKVV
                     DLGHRINPNFKIGCMIQYTPAYTKTCHPNDALAKRFYNIQNYYYTDVMVKGEYTSLCY
                     SQLKRLGVDLTISKEEQEILKKGKVDYIAFSYYFTYVVSYDENQFVIDKKNKYLQTGQ
                     WNRTIDPIGLRVALNELYDRYQVPLFVVENGIPLDDKIDSNNEIIDEERIAFFRNHIM
                     QIKKAIHEDFVNVIGYTTWGPIDIVSVSTGEMNKRYGFIYVDKDNSGKGTLQRFKKKS
                     FAWYKKVIESNGECL"
     gene            complement(4201..>5265)
                     /locus_tag="PLK68_03955"
     CDS             complement(4201..>5265)
                     /locus_tag="PLK68_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89596.1"
                     /translation="SKIFSTVNFTCISCMALWLVFLMGYQLGEKHNKLLSALITTVCF
                     LVVSDQENLLSNLGASALFLAFFVAILSNLIFVNLMRFEKIKIKLPDSVPPMIARSFN
                     SLIPAWITITVFGIFAGTLYGATGLYINTIIYNVIQVPFNQIIGSQIGVSIIVVLSQL
                     LWWTGIHGHMAMNVIAKPVRTAALAANIAAVSAGLVPEEFYTLAYVHLFTNLGGSGIV
                     ISLALAILVFSKRQEYKDITKLSMIPLICGISEPMVFGLPIMLNPIFLIPFVLVPLIT
                     ANIGYYAITSGFLVSSYVESVAGMPMFIQQFLGFSGQWQAILLTVVVLVIGFLSYAPF
                     VILSNKKTESELLLNKENEV"
BASE COUNT         1902 a          924 c          590 g         1849 t
ORIGIN      
        1 gccgacttat tggaagtcgg ttttttaaat aggtatttta taaaaataaa attaagagat
       61 tataataaat aattgctatt aaacaaaaca ataatgtttt tgggattgtt ttacgtatca
      121 aatctcctaa actgacttta aaataatctg ctacaacaac taaacaaata tgagtaggtg
      181 ataactgact tgctccatga catatacaca ttaacaatac cattaatggc atgccaccct
      241 gaatagttgc aaatgctatt ggtgttccta aagctataat tccacttgat ccactaatga
      301 tccctccaat gaaaaataat ataacaaaaa ccaagtatgg tggaattggc aaacttagta
      361 ataaatctgg taatacattt aaaactcctg tataagcgat aaattcttta aatactaaaa
      421 ctaaaaaggt attcactatc attttctttt caaatgcctt tgtaatcaat ggcagcaatt
      481 cattgatttt aaatctataa atcatacaac atagagcaat gactatcaat actgcagaaa
      541 caattgatag atgaaatagt aaaataagca ccaaaatcat taatagtgac cataaatgtt
      601 gaaacaaatg aataaaatct ttcattctat tttggctttt aggtgtattc gtacttgatg
      661 gtattttcac taatattgaa aaatagccaa ttaaagcaag agcgaaaaca ggtataatca
      721 tcccaatcat aaattctgat aatggaacac ctgaaatatt cgccattaat aaaacagctg
      781 catatgtagg taaggtgctt tcaggtatgt gcctaaacca actagcaaca aatgcctgat
      841 cttttggttt taagtaccca tcggttgcat ctttaacaat atccccacat aaaatcattg
      901 cagcagcgga tggtagtaac cctataaata atggagcacc tccagcattg attcttctgt
      961 tatggaaaag cccatttaaa tcttgctgag ctaatttgat ttgttgtctt ttttctaaca
     1021 ttttttgcag gaaagtaata agatataatg aaattagaat ttgtaatgaa ctccaatttg
     1081 taaatacatg agttatctgt ataaaaatat cattaaaagg aattttataa agaataacca
     1141 tcataattaa tccaccaata attgattgat ataatggacg acgaaacatt aataacaata
     1201 caataatcaa aaaaacaatt cccaaataaa caactaacat ataattcctc ctctttaaaa
     1261 ttctcactta ttttatacta gttaataaat gatttgaaga tactcataaa tttatgagta
     1321 tcttataaat ttgttttata ttctagatag gaatatatag attttttgtt tccatttgta
     1381 tgcacctata cttataaaca actttcattg caaatgaaaa agcataactg ataatttcag
     1441 aataagtcct atactaaaac ttaaacttaa taaaagttat gcttttctta tgtttaattt
     1501 tatctttaaa aattaaaatt acgttgattt tatcactatt tatttcttag tttcatctgc
     1561 aggaacaaga taacttcttg aatagattcc acctgttggt ggtaatccat attccaatgc
     1621 ttctacataa tctaaatccg ctgtcttcaa aagtatattt cctcttttta tcattctctt
     1681 ttataataac ttctggatat tcaaattcat tatcatccat aaactcgtca agctgatctt
     1741 gattattaac tcttgttatt aatttatcat ttagatcata aatctctatc atacttttat
     1801 ccccaaaaaa cagtttttta taacagcaat acggtattta tgatattgga aatgtatttt
     1861 tacataagat attttctttt gaatacctga atttttccat aaaaaattga attcatcatt
     1921 acaaatctgt tcgcatttta cgattttttc aataatcggt ctgcactctt ccttcatttt
     1981 tttatatgag caaccgcctt gtcattcgct ctacttaatt ttataagata atcttacagt
     2041 ttattatata gatcttcatt cataaattcg ctaaaatact gacagttttt cacgtctata
     2101 atatttatta agtttcttgc tataatttct ctgtctctca caataatcac tatttaatat
     2161 taacgcatat attttaataa tatcaaccac tctttaatca ttaactatac tgttttcttg
     2221 atatttttat ttttataaat ttgaataaat attgtttttg ctatcagtaa gcgaaaaact
     2281 ttaacaacct taatacaaac taaccaaatt tatttgctaa aaattttaaa agaaatattt
     2341 attattaata tagtcaaagc tattttaaat aaagaattaa aataggacac attataatat
     2401 ataatagcat attcaaaact atttcacatc caaaatctaa aaaatgacaa actatacctt
     2461 ttctctaccc atcatttttg atgcaaatac cttatcaggt acattttcaa tagggatagc
     2521 agctcccgtc actggagcat acaatacaac agattctttt ttttaacaaa ctaaacatta
     2581 ttctaacctc caacaaaaaa actctctgta tgcaaaacaa acacaacaat agttatattt
     2641 agtaatgcct cccagagagt aacaatcttt ttttatttta caagtatatt aacaatttct
     2701 ataagtgttg tcaatggtaa tcttttaatt ggataaaata atcttattta aattgctaat
     2761 cttttaacaa taaaactgtt tttattcctt cttaaggcaa cgttatttac aaacattcac
     2821 cattgctctc aattactttt ttataccatg caaaagattt ctttttgaat ctttgtaaag
     2881 ttccttttcc actgttatcc ttatcaacat atataaatcc atatctttta ttcatctcac
     2941 cagtagatac agaaacaata tctataggtc cccatgttgt atatcctatt acgttcacaa
     3001 aatcttcatg tattgccttt tttatctgca ttatatgatt acgaaaaaaa gcaattcttt
     3061 cttcgtctat aatctcatta ttgctatcaa ttttatcatc tagaggaatt ccattttcaa
     3121 caacaaacag tggaacctga taacgatcat ataattcatt taaagctact ctaagtccta
     3181 taggatcgat tgttcgattc cattgtccag tttgcaaata tttatttttc ttatcaataa
     3241 caaattgatt ttcgtcataa cttactacat atgtaaaata ataactaaaa gcaatgtaat
     3301 ctacttttcc tttttttagt atttcttgct cctctttgct aatagtaaga tcaactccta
     3361 gacgctttaa ttgagaataa caaaggcttg tatattctcc cttaaccatt acatctgtat
     3421 aatagtaatt ttgaatatta taaaaacgtt ttgctaatgc atcatttgga tgacacgttt
     3481 ttgtataagc aggtgtatac tgtatcatac atcctatctt aaaattagga tttatacgat
     3541 gtcccaaatc cactaccttt gctgatgcaa taaacatatt atggcttgca ataactttta
     3601 ctttcttatc attctcatcc tctttaaatt ttaacccggc ctgcaaaaag ggatcttttt
     3661 tattcatcgt ttcattaatt tcattaaatg tcatccaata tataacttta tctttatatc
     3721 ttttaaaaac cacattcgca tattttacat aaaaatcaat cagttttgga tttctccaag
     3781 aaccataagc ttccaccaaa tgtaaaggtg tctcaaaatg cgacaatgta acaataggtt
     3841 gaatattata ttttaacagc tcatcaaaca ctttatcata aaattgtaag ccgcattcat
     3901 taggaatttc atcatctcca ttaggaaaaa ttcttgccca actaatcgac attcttaagc
     3961 atttaaaacc catttgtgca aacaaagcaa tatcctcttt gtatcgatga taaaaatcaa
     4021 tagcctcatg gcttggataa tatactccct catgaatctt ttcatctact tgtctaggga
     4081 ttccttgctg tgatccacgt acaacatctg ctatagataa tcctttccca tctaaattat
     4141 atcccccttc gcactgattt gctgctaatg caccaccaaa taaaaaactt tccttcatat
     4201 tcatacctca ttttccttat ttaaaagtaa ctccgattct gtttttttat ttgaaagtat
     4261 aacaaatggt gcataactca aaaaacctat aacaagaaca actacagtta acaaaatagc
     4321 ttgccattgc ccactaaacc ctaaaaattg ttgaataaac ataggcatac ctgctacaga
     4381 ttccacataa gatgatacta aaaatccaga ggtaattgca taatatccga tattagcagt
     4441 aataagcggt accaatacaa atggtattaa aaatatagga ttcagcataa tcggtaaacc
     4501 aaaaaccatt ggttcagaaa ttccacaaat caatggaatc attgataatt ttgtaatatc
     4561 tttatactct tgtcgtttag agaatactaa aatcgctaaa gctaaagaaa tgacaatccc
     4621 tgaaccacct aaatttgtaa ataagtgaac atacgctaaa gtataaaact cttcaggtac
     4681 taacccagca gacactgcag caatatttgc cgctaaagct gctgtacgca caggcttcgc
     4741 aataacattc attgccatat gtccatgaat tcctgtccac caaaggagtt gtgataaaac
     4801 aacaataata ctaacaccta tttgagaccc aataatttga ttaaatggta cttgtattac
     4861 attatagata attgtattga tatatagtcc tgtagctcca taaagtgtcc ctgcaaagat
     4921 accaaaaaca gtaattgtaa tccaagcagg tatcaaagaa ttaaaactac gcgctatcat
     4981 aggtggtact gaatctggta attttatttt tattttttca aatctcataa gattaacaaa
     5041 tattagattt gataaaatag caacaaaaaa agccaaaaat agggctgatg cacctaaatt
     5101 acttaacaaa ttttcttgat ctgacaccac taaaaaacat actgtagtaa ttagcgctga
     5161 taataattta ttgtgcttct ctcctaattg atatcccatt aaaaaaacta gccacaaagc
     5221 catacaggaa atacaagtaa aatttacagt agagaatatc tttga
//
LOCUS       DAOMTA010000098         5256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_333_length_5256_cov_2.083253, whole genome shotgun sequence.
ACCESSION   DAOMTA010000098 DAOMTA010000000
VERSION     DAOMTA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_5256_cov_2.083253"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLK68_03960"
     CDS             <1..285
                     /locus_tag="PLK68_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRM89597.1"
                     /translation="QLAKNLCLSFEKSLDRKIAEVFIAQKLEDDYTKDEILEMYLNIT
                     YLGEGNYGIKEASNYYYNIEPIELNEEQAKVLVKTLKAPSVYNPSKLAEN"
     gene            513..1232
                     /gene="larE"
                     /locus_tag="PLK68_03965"
     CDS             513..1232
                     /gene="larE"
                     /locus_tag="PLK68_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRM89598.1"
                     /translation="MAYSGGCDSNFLYQVALQTLSKENVLAVLCVGKMMSKEDIDSAR
                     MMAGKGQFIEVPLDVFTIEQFTNNRKDRCYHCKKQVMAKVIEAAQSHDFKITLDGKNA
                     DDEKVYRPGMKACEELGIVSPLALVGLTKAEIRQYSKELQIETYDKPANACLASRFPY
                     DTHLTKEKLEVVDQAEALLHQKGIYYARVRVHDKLARIEVERNNFNLVNDEELILAIK
                     ELGFDYVTLDLEGITSGSYDR"
     gene            1222..1632
                     /locus_tag="PLK68_03970"
     CDS             1222..1632
                     /locus_tag="PLK68_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HRM89599.1"
                     /translation="MIGSEMEVVVIDGQGGGIGRSVIEALKKEVSGTFIIAVGTNSTA
                     TNNMKKGGADAVATGENAIIYNAKNAQIIIGPIGFVFANSMYGEVSPAMAAAISCSEA
                     QKYFIPVSKCTGHVLGVEPKSIQEYIRDLVNILK"
     gene            1690..>2511
                     /locus_tag="PLK68_03975"
     CDS             1690..>2511
                     /locus_tag="PLK68_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRM89600.1"
                     /translation="MNSVLMHMADGLISVNIGVIFMIISFIMIGFSIKKINQEHDDRK
                     IPLMGVMGAFIFAAQMINFTIPGTGSSGHIGGGILLCLILGQYPAFLSLCSVLIIQCL
                     FFGDGGLLALGCNIFNMGILPCFVAYPLIVKPLLKKNITSSRVLLASILGVVVGLEMG
                     AFAVVLQTLASNITELPFTSFVVLMLPIHLAIGLVEGLITSTVVLYVLKDKREIVDHA
                     LNDKHFGTLNIRRQVAVFAVLTILVGGVLSLYASSNPDGLEWSIEGVTGTDEIEAS"
     assembly_gap    2512..2521
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2522..2702
                     /locus_tag="PLK68_03980"
     CDS             <2522..2702
                     /locus_tag="PLK68_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HRM89601.1"
                     /translation="STKDTLAKVQSSTSFLPDYNFSGSDSKLGVSVSGIIGGAATLIL
                     IGGIGYIVVKKKKYE"
     assembly_gap    2858..2875
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2876..3368
                     /locus_tag="PLK68_03985"
     CDS             <2876..3368
                     /locus_tag="PLK68_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRM89602.1"
                     /translation="MISLSKVSFMDLFKRSLIGLPISLCIGFSNLLFSTTIINFYGVN
                     ISTGVLLFITIIIKNILCLMAVFLLMATTKFDSITCELVHLKIPSIFVLQLVMIYRYI
                     FVLVEEALTMIQAYQLKNPQSKGIAFKDMGSFVGSLLVRSFERSNEVYNAMKCRGFDV
                     KQD"
     gene            3429..4145
                     /locus_tag="PLK68_03990"
     CDS             3429..4145
                     /locus_tag="PLK68_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HRM89603.1"
                     /translation="MIEINNLNVLYNNSIRALDNVSIKIEQQKCIAIVGENGSGKSTL
                     LSSLLGLVETQGEIKINGVMLSKETLIQIREVAGLVFQNPDHMIFLPTVRDNLAFGLI
                     NQKLDKIEIDKRIAEFAKLFKIEDLLDRMANHLSGGQKRMVGLASVVVMKPEILLLDE
                     PSAFLDPKSRRIVINMLKELPQQIIFATHDLDMVLDLCDEIILLNNGKIVSSGNPCEI
                     LANQELLEANGLELPQRYQR"
     gene            4149..4889
                     /gene="larB"
                     /locus_tag="PLK68_03995"
     CDS             4149..4889
                     /gene="larB"
                     /locus_tag="PLK68_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRM89604.1"
                     /translation="MKVKEILEMVENHEISVDEAAILIDNPIDYATIDYNRKRRTGTP
                     EIIYGSGKTKEQIAGIIKNMLEHDQIDILATRVDATKAAYLKKLYPNFNYDKEAKTFI
                     LKQSETIQNKGMIVVVCAGTSDIPIAREAVLTAEFLGNEVNLISDVGVAGIHRLFNKM
                     DVIKRANVIIVVAGMEGALASVVGGLVDKPVIAVPTSIGYGANFNGLSALLSMLNSCA
                     SGVSVVNIDNGFGAGYMAHTINCLGGKR"
     gene            4886..>5256
                     /locus_tag="PLK68_04000"
     CDS             4886..>5256
                     /locus_tag="PLK68_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HRM89605.1"
                     /translation="MKVLYFDCSSGISGNMTLGALMELIDEPDYLQKELAKLNIDGYH
                     LHVTKTKKNGITGTYVDVHLEEHPHHHDHEHHHHEHHHHEHHHHVHRNLFDVNKIIDD
                     SGINERAKALAKRIFLRVALA"
BASE COUNT         1780 a          597 c         1074 g         1777 t
ORIGIN      
        1 cagttagcta aaaatctttg tttatctttt gaaaagagct tagaccggaa aatagctgaa
       61 gtttttattg ctcaaaaatt agaagatgat tatacaaaag atgaaattct tgaaatgtat
      121 ttgaatatta catatttagg tgaaggaaat tatggaatta aagaagctag taattattat
      181 tacaatattg agcctataga acttaatgag gaacaggcaa aagtattggt aaagacattg
      241 aaggccccta gtgtttataa tccttctaag ttagctgaaa attaaagtga tagtgatgaa
      301 gtgaattttc tatgttgaat tagcaatgag ggtttgcttc atattgaaat cacttttttt
      361 attttctaaa taaaaaatac ttgtcttaaa gtgtacttta gggtgtatag tgtagttgcg
      421 atcatagcat ggtctaatta gtaaatagat ggggattgtg atattattat gtataaaggt
      481 gaagagatga gtaagttaga taaaaagttg caattgccta tagtggtggc tgcgattcaa
      541 attttttata tcaggtagca ttacaaacct taagtaaaga aaatgttcta gcagtattat
      601 gtgtagggaa aatgatgtcc aaggaggaca tcgatagtgc tagaatgatg gctggtaaag
      661 gtcaatttat agaagtgccg ttagatgtat ttacaatcga gcaatttaca aataatcgta
      721 aagatcgatg ttatcactgt aaaaaacaag taatggcaaa agtaattgaa gcagctcaaa
      781 gccatgattt taagataacc cttgatggta aaaatgctga tgatgaaaaa gtttatcgcc
      841 ctggaatgaa agcttgcgaa gaattgggaa ttgtatcacc gttagcacta gttggtttaa
      901 caaaagctga aataagacag tattcaaaag aattacagat tgagacatat gataaaccgg
      961 caaatgcttg tttagcttca cgttttcctt acgatactca tctcactaaa gaaaaattag
     1021 aagtggtcga tcaagctgag gctttacttc atcaaaaggg gatttattac gctcgtgttc
     1081 gagttcatga taaattagca cggattgaag ttgaacgaaa caattttaat ctagttaatg
     1141 atgaagaatt aatcttagca ataaaagaac ttggatttga ttatgtaaca ttagatttgg
     1201 aaggcataac tagtggaagt tatgataggt agtgaaatgg aagttgtagt tattgatggt
     1261 caaggtggag gcattggccg aagtgtaatt gaggccttaa aaaaagaagt tagtggtact
     1321 tttattattg cggtaggaac aaatagtact gcaaccaata atatgaaaaa aggtggagct
     1381 gatgctgtcg caacgggaga aaatgcaatt atttataatg ctaaaaacgc tcagataatt
     1441 attggaccaa ttggttttgt ttttgctaac tcgatgtatg gtgaagtttc accagcaatg
     1501 gcggcagcaa tcagctgtag tgaagctcaa aaatatttta ttccagttag taaatgcact
     1561 ggacatgtct taggtgttga gccaaaatca attcaagaat atattagaga tttagttaat
     1621 attttaaaat agatcccaag tacaagaagt actagaagaa gcacagtagt gtttgtggaa
     1681 agggaaatta tgaattcagt attaatgcat atggcagatg gtttgatttc tgttaatatt
     1741 ggggttattt tcatgataat tagttttatc atgatcggtt tttcaattaa aaaaattaat
     1801 caagaacatg atgatcgaaa gattccttta atgggagtaa tgggagcatt tattttcgct
     1861 gctcaaatga tcaattttac gattccggga acaggaagta gtggtcatat cggtggagga
     1921 attttattgt gccttatttt aggacaatat cctgcctttt tatcattatg ttctgtttta
     1981 attatacaat gcttattttt tggtgatggt ggtttattgg cgttaggatg taatattttt
     2041 aacatgggga tattaccatg ttttgtagct tatccattaa ttgttaagcc actattaaag
     2101 aaaaatataa cttcttcaag agtattactg gcaagtattt taggagtagt agtgggatta
     2161 gaaatgggtg cttttgcagt ggtcttacaa actcttgcaa gtaatattac ggaattacca
     2221 tttacttcat ttgttgtatt aatgttgccg attcatttag caattggtct agttgaaggc
     2281 ttaatcactt caacagttgt cttatatgta ttaaaagata aacgtgaaat agttgatcat
     2341 gcacttaatg acaagcattt tggaacattg aatataagaa gacaggttgc ggtttttgct
     2401 gttttaacaa tcttagttgg tggagttctt tcattatatg catcaagcaa tcctgatggt
     2461 ttagagtggt ctattgaagg agtcacagga accgacgaaa ttgaagcaag cnnnnnnnnn
     2521 ncagtacaaa agatacttta gcaaaggttc aaagttcaac ttcattttta ccagattata
     2581 attttagtgg cagcgacagt aaattaggtg tttcagtatc agggattatt ggtggtgcag
     2641 caactttaat tttaattggt ggaattggat atatcgtagt aaaaaagaaa aaatatgaat
     2701 aaacttaaag gctcactttt aaaatttggc tatattgaaa agttagctaa tggtgattca
     2761 ctgattcatc gtttaaatcc acttttaaag attattttag cactaatgta tataattttt
     2821 gttatttcga ccagctgctt agattttttt gaattacnnn nnnnnnnnnn nnnnnaatga
     2881 tttcacttag taaagtatca tttatggatt tatttaaacg aagtttaatt ggattaccta
     2941 tttctttgtg tattggattt tcaaatttac tattttcaac cacaataatt aatttttatg
     3001 gtgttaatat atcaactggt gtacttttat ttattacgat cattataaaa aatatcttat
     3061 gtttgatggc agttttttta ctaatggcaa caactaagtt tgatagtatt acttgtgaat
     3121 tagtgcatct taaaatacct tcaatttttg ttttacaatt agtaatgatc tatcgttata
     3181 tttttgtttt ggttgaagaa gcgctaacaa tgattcaagc ttatcaatta aaaaatcccc
     3241 agtctaaagg aatagctttt aaagatatgg gtagttttgt gggaagttta ttagttcgga
     3301 gttttgaacg aagtaatgaa gtttacaatg caatgaaatg tcgtggcttt gatgttaaac
     3361 aagattgaaa attatttttt attaatgatg gcagtaggag ttttaatgat ggtaaaggtg
     3421 gtattttagt gattgagata aataatttaa atgttcttta taataattca attagagctc
     3481 ttgataatgt gtcgataaag attgaacagc agaagtgtat tgctattgtt ggagaaaacg
     3541 gctcggggaa atcaacttta ttatcaagct tattggggct ggtggaaacg caaggtgaga
     3601 ttaaaattaa tggagttatg ttatcaaaag aaacattgat acaaataaga gaggtagcag
     3661 gtcttgtttt tcaaaatcca gatcatatga tttttttacc tacagttcgt gataatttag
     3721 cttttgggtt gataaatcaa aaactggata aaattgaaat tgataaaaga attgcggaat
     3781 ttgcgaaatt atttaagatt gaagatttgc tggatcgaat ggctaatcat ctttctggag
     3841 gacaaaagcg aatggtaggc ttagctagtg tggtggtaat gaaaccagag atattattat
     3901 tagatgaacc atcagcattt cttgatccga agagtcgaag gatcgttatt aatatgttaa
     3961 aagaattgcc ccagcaaatt atttttgcaa cccatgattt agatatggta ttggatttgt
     4021 gtgatgaaat aattttatta aataatggaa agatagtatc tagtggtaat ccctgtgaaa
     4081 ttttagctaa tcaagagtta ttggaggcaa atgggttaga gttgccacaa cgatatcaga
     4141 ggtagacgat gaaagtaaaa gaaatattag aaatggtaga gaaccatgag atttctgttg
     4201 atgaagcagc tattttgatt gataatccaa ttgattatgc gacgattgat tataatcgta
     4261 aacgtcgtac gggaacaccg gaaatcattt atggatctgg taaaacgaaa gaacagattg
     4321 ctggtattat aaaaaatatg ttagaacatg atcaaattga tattttagcg actagagttg
     4381 atgcaacaaa ggcggcttat ttaaagaaat tatatccaaa ttttaattat gataaagaag
     4441 ccaaaacttt tattttaaaa caatcggaaa ctatacaaaa taaaggaatg attgttgttg
     4501 tttgtgcagg aacatcagat attccaattg ctagagaggc tgtgttaaca gctgagtttt
     4561 taggaaatga agttaatttg attagtgatg ttggtgttgc tgggattcat cgactattta
     4621 ataagatgga tgtgatcaaa cgagcaaatg ttattatagt tgtagctggt atggaagggg
     4681 ctttagcatc ggttgttgga gggttagttg ataaaccagt cattgcagtg ccaacatcaa
     4741 ttggttatgg tgctaatttt aatggtcttt cggcattatt atcaatgtta aacagttgtg
     4801 ctagtggtgt tagtgtagtt aatattgata acggttttgg agctgggtat atggctcata
     4861 cgattaattg tttaggagga aaacgatgaa agtattatat tttgattgta gtagcggaat
     4921 aagtggtaat atgactttag gtgcattaat ggaacttatt gatgaacctg attatttaca
     4981 aaaggaatta gctaaattga atatagatgg ctatcattta catgttacta aaacaaagaa
     5041 aaatggtatt actggtactt atgtagatgt tcatttagag gaacatccac atcatcatga
     5101 tcacgaacat caccaccatg aacatcacca ccatgaacat catcaccatg ttcatcgtaa
     5161 tttattcgat gttaataaaa ttattgatga tagtgggatt aatgaaagag ctaaagcatt
     5221 agcaaaaaga atctttttaa gagttgcgtt agcaga
//
LOCUS       DAOMTA010000099         5251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_334_length_5251_cov_2.828907, whole genome shotgun sequence.
ACCESSION   DAOMTA010000099 DAOMTA010000000
VERSION     DAOMTA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_5251_cov_2.828907"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLK68_04005"
     CDS             complement(<1..283)
                     /locus_tag="PLK68_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM89606.1"
                     /translation="MKPTSFENAIRLQFDTLMKKVIDGIIKNYEKELDRRSNREIPFC
                     ELPKIVVNSFPVFDDYELDVTIFDVYGMEARVSGNELCKALQQLPERKRN"
     gene            852..1295
                     /locus_tag="PLK68_04010"
     CDS             852..1295
                     /locus_tag="PLK68_04010"
                     /inference="COORDINATES: protein motif:HMM:NF021219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HRM89607.1"
                     /translation="MLNKKSLFTKLAAVSMVASMMLATPVMAATNDTGDKDAQNGIVK
                     TTYEDENGTWTEGMIGGNPEGVETCWLAYGPLYQYPSEGGTWQYGFWNAKVRSYYTVN
                     RCHGSTVKLNGKTSRSVNTASGKKSIAELWAIQSDSNDQYFYRVC"
     gene            1411..3417
                     /locus_tag="PLK68_04015"
     CDS             1411..3417
                     /locus_tag="PLK68_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89608.1"
                     /translation="MNKHGIRLISFILALFSFIMIFQNLMNDQQQLPLGTTEQLVLNI
                     AETGTTKEQLISELDNITNKHNATLVKVVIPSDEYKNKKDIIYFGDEKPKSSDIIIDN
                     DNIKWLDSSLEGKLISSKDIGNRPLYGNYAVKGNQNFKQDIENWANDNGITIQWLRKV
                     SFIKSLYYNLVHNGVGNVILTVLLLFISVLLTWFISHSKGRSIRLLSGVDIKKIHIED
                     TLTLSKMFICSYLISLIAFWVYIGITNDFKQITLLLSKDIITLLVLVVISALISFGIS
                     MIVKPKTEHLAKREIPFKKFRMLSIGTRIMSIILALLIVPSTITSAYILQQLSNEYSL
                     WENMQSIVSISFSDLDSLSTDKMLPYVDDFFANMADNDNLSLSLSIDKSIQLSEEEYG
                     GYDHIIVTDKSWVDSFDIGIEENKSGGELNKIKLNDLDKPLQDFINVQMPLWTKTEEI
                     QPSGINFYEFSGDKFLALPPNVGYGGSTIQAKNPLVILVDNPVQSLNTLGFMLPACSS
                     GNVVFPDENLLRTELAKTPVKDYVVSIDTIADVALSQAQKFAKEAVFYVIACILIFVS
                     MIFAGILTAQLWVSENRKRIFTLHTFGKSYKEIIMQTFVHESFIAIITVIIGAVISFV
                     IRRPEIITVLSVSVIILILYCLSNFVAYHICTRQAFYKVATRNE"
     gene            3410..4063
                     /locus_tag="PLK68_04020"
     CDS             3410..4063
                     /locus_tag="PLK68_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM89609.1"
                     /translation="MSKKWGVKMEIKMNSISVVIKGKRIFSNITDTMNSGDMVALTGS
                     SGCGKTTLLNCLGLIQPIESGSILINGKDTSKWNDKDKTKFWHKYATFIYQDYGIIED
                     ETVAYNITLDKSKAKSKAVKNILDKVGLNGRDSELAIVLSGGEKQRIGIARAILKNAS
                     IIYADEPTASLDSENRQIVISLLKECASNGAIVILATHDDRLVSECNKVISLNKNRN"
     gene            4075..5211
                     /locus_tag="PLK68_04025"
     CDS             4075..5211
                     /locus_tag="PLK68_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HRM89610.1"
                     /translation="MKKMFSVLLITTIITTLGSPVYASSQNPSDLQECIESSMFYNNE
                     YRLFNEFTYQGMEINLFPETTITKEEALIKYKEAYNYIAEKASALGIVADLDNYEFQQ
                     YIKGYAIPTSTNKELNDKLAEFAEFIDIYENAESNKKIISCLKEKEFNSEELELLLPI
                     SKSSTELSNKITPSDNRESNGYSSNKATQYAKQWWNRTNNTDFPYYANYYKQDLNSND
                     YNDLDENAEGQSAIRRGWSDCTNFVSQCLSYGGFQYRKSGLILPHRQSKNWYYDNGKP
                     SHTWGGANNFYQHWESRAGIAKTSDLLTAGDVISVDFTKDGDIDHTAIITSNINNTNE
                     SKLLTQHSIDRSEFLPTGDYFSLANLYSQDYKIYGYEMDKAPIK"
BASE COUNT         1947 a          687 c          891 g         1726 t
ORIGIN      
        1 tatttctttt tctctctggt aactgttgta acgctttaca tagttcatta cccgatactc
       61 gggcttccat tccatacaca tcaaaaatag ttacatcaag ttcgtaatca tcaaaaacag
      121 gaaaggaatt aaccacaatt tttggtagtt cacaaaacgg gatttcacga ttagaccgtc
      181 ggtctaattc tttttcataa ttctttatga ttccgtcaat aacttttttc attagcgtat
      241 cgaactgtaa gcgaattgcg ttttcaaaag aagtcggttt catgttcaca cctcctccct
      301 gcacaaagcg gagaatcttt atcccccttt ccgcaccaac actgtacagg caggtgtgat
      361 ttgctaacct aatttcaaaa aaagtgaaaa aaagtttttg agcatgaaaa aagtccgtac
      421 aaaacaaatt gttgtagcgg acaattttaa aagtatttat taaaattttt gggatattaa
      481 aacaactata aaaatgaaag tttgtatcat tatcaataac tttatttagt gtacaattaa
      541 aatatgtttt cccatttgta accaatctcc tgtaaaaaaa tataaacgat acatattaca
      601 ttaaaattat aatctatcac atcaaaaaaa ttgagcgaaa acagtcgaaa tgaaaaaata
      661 atataaatat aaaatatttt tctatttgat tctatataat tatagcatta aattgacaaa
      721 attcgtcatt ttatgaaagc gtttattttt tttataattt catattgtta gatattgaca
      781 attcagtggt ttaggactat cataaaggtg tagaataaag tagctaaaag gagccaaaag
      841 gaggaatggt catgttaaac aaaaagtctt tattcactaa actagcagct gtgagcatgg
      901 tagcttcaat gatgttagct acgccagtaa tggcagcgac gaatgacaca ggcgacaagg
      961 acgcacaaaa tggaatcgta aagacaactt acgaggacga aaatggcaca tggactgaag
     1021 gtatgattgg tggtaatcca gaaggagttg aaacttgttg gttggcatat ggaccattgt
     1081 accaatatcc aagtgaaggt ggaacatggc aatacggttt ctggaatgct aaagttcgtt
     1141 cttactacac agttaataga tgtcatggtt caacagttaa gttgaatggt aagacatcac
     1201 gttcagtaaa tactgcatca ggtaagaaat ctatcgctga attatgggct attcaatctg
     1261 attctaatga ccagtatttc tatcgtgttt gttagttaag tatcatattt attgtttgat
     1321 agcttaaatg acaaattaga ttttaggctt cggtgcttgt catcgaagcc tattttattt
     1381 aatgaatagg ataatggagt tgatattttt atgaataaac atggcattag attgattagt
     1441 tttattttag ctttattttc attcattatg atctttcaga atttaatgaa tgaccaacag
     1501 caattacctt taggtacaac agaacaactt gtattaaata ttgctgaaac tggtactacg
     1561 aaagaacaac ttatttcaga gttagataat atcacgaata agcacaatgc tactttagta
     1621 aaagttgtta ttcctagtga tgaatataaa aataaaaaag acatcattta ctttggagat
     1681 gagaaaccaa aaagttctga cattattatt gataatgata atattaaatg gttagattca
     1741 agtttagagg gaaagttaat ttcatctaaa gatattggta atagaccttt atatggaaat
     1801 tatgcagtaa agggaaatca gaattttaaa caggatatag aaaactgggc taatgataac
     1861 ggaattacta ttcaatggtt gagaaaagta tcgtttataa aaagtttgta ttacaattta
     1921 gttcataatg gtgttggaaa tgttatttta acagtattat tattattcat ttcagtatta
     1981 ttgacatggt ttatttcaca ctcaaaagga agaagtataa gattattgag tggagtagac
     2041 attaaaaaaa ttcatattga agatacatta acactatcaa aaatgtttat atgcagttat
     2101 ttaatttcac tcatagcatt ttgggtatat attggaatta ctaatgattt taaacaaatt
     2161 acattattgt tatctaagga tattataaca ctattggtat tggttgttat ttcagcattg
     2221 attagtttcg gtatttcaat gattgtcaaa ccaaaaacag aacatcttgc gaaaagagaa
     2281 attcctttca aaaaattcag aatgctcagt attggcacta gaattatgtc aataatatta
     2341 gctcttttga tagtaccaag caccataaca tctgcttaca ttttacagca gttatctaat
     2401 gagtattctt tatgggaaaa tatgcaaagc attgtaagta tatctttcag cgatttagat
     2461 tcattaagta cagataaaat gttgccatat gttgatgatt tttttgcaaa tatggcagat
     2521 aacgataatt tatccttatc tttatctatt gataaatcta tacaattaag tgaggaagaa
     2581 tacggtggtt acgaccacat aatagttact gataaatcat gggttgattc ttttgatatt
     2641 ggaatagaag aaaataaaag tggtggagaa ttgaataaaa ttaaattgaa cgatttagat
     2701 aaaccactac aggactttat aaatgttcaa atgcctttat ggactaagac agaagaaatc
     2761 cagcctagcg gaattaactt ctatgaattt agcggagata aatttttagc tttaccgcca
     2821 aatgttggct atggtggaag cactatacaa gctaaaaatc cattagttat attagtggat
     2881 aatccagtac aatcattaaa tacattaggt tttatgttac ctgcttgttc aagtggtaat
     2941 gttgtttttc cagatgaaaa tttattgaga actgaattag caaaaacacc tgtaaaagat
     3001 tacgttgttt cgatagatac tattgctgat gttgctttat cacaagctca aaaatttgca
     3061 aaagaagctg tattctatgt gatagcttgt attctaattt ttgtatctat gatttttgca
     3121 ggtattctta ccgcacagtt atgggtaagc gaaaacagaa aacgaatatt tacgttacat
     3181 acattcggaa aatcatacaa agaaataata atgcaaacct ttgtgcatga atcatttatt
     3241 gcgataatca cggtaatcat tggagcagta atttcttttg ttataagacg accagaaatt
     3301 attacagttt tatcagtgag cgttattatt ttgattttat attgcttgag taattttgtt
     3361 gcttatcata tatgtactag acaagcattt tacaaagtag caacaagaaa tgagtaaaaa
     3421 atggggtgta aaaatggaaa ttaaaatgaa tagtataagc gttgtaatca aaggaaaacg
     3481 cattttctca aatatcacag acacaatgaa tagtggagat atggtagctc tcacaggttc
     3541 ttctgggtgt ggaaaaacga cattattaaa ttgtttaggt ttaatacagc caatagaaag
     3601 tggttctatt ttgataaatg gcaaagatac ttcaaagtgg aacgataagg ataaaacgaa
     3661 attttggcat aaatatgcga catttattta tcaagattat ggaattatcg aagatgaaac
     3721 tgtagcatat aacattacat tagataagtc aaaagctaaa agtaaagctg tgaaaaacat
     3781 attagataaa gttggtttaa atggtagaga ttctgaacta gcaatcgtac tctctggtgg
     3841 agaaaagcaa cgcataggta tagcaagggc aatattaaaa aatgcttcta ttatctatgc
     3901 tgatgagcct actgcttctc ttgattctga aaatagacaa atcgtgatta gcttattaaa
     3961 agaatgtgct tcaaatgggg caatcgttat tttagctaca catgatgata ggcttgttag
     4021 tgaatgtaat aaagtcataa gtttaaataa aaatagaaat taggagtaat gattatgaaa
     4081 aaaatgtttt cagttttatt gattacaact atcattacta cattaggtag tcctgtttat
     4141 gcaagtagtc aaaatccaag tgatttacaa gaatgtatag agagctctat gttctataat
     4201 aatgaatata gattattcaa tgaatttaca tatcaaggaa tggaaatcaa tttgtttcct
     4261 gaaacaacaa tcactaaaga agaagctctt attaagtata aagaagcgta taactacata
     4321 gcagaaaaag ctagtgcact aggtattgta gcggatttag ataactatga atttcaacaa
     4381 tatattaaag gttatgctat accaacttct actaataagg aacttaatga taaactagcg
     4441 gaatttgcag aatttataga tatttatgaa aatgcagagt ctaataaaaa aatcatttct
     4501 tgtttaaaag aaaaagaatt taattctgaa gaacttgaat tgttattacc aataagtaag
     4561 agttcaactg aattatctaa caaaataact cctagtgata atagagaatc aaatgggtac
     4621 tcttctaata aagctactca atatgcaaag caatggtgga atagaacaaa taatacagat
     4681 tttccatatt atgctaatta ttataagcaa gaccttaatt ctaatgatta caatgattta
     4741 gatgaaaatg cagagggaca gtctgcaata agaagaggtt ggagcgactg tacaaatttt
     4801 gtttcacaat gtttaagtta tggtggtttt caatatagaa aatcaggatt gattttacca
     4861 catagacaat ctaaaaactg gtattatgat aatggaaagc catcacatac atggggtgga
     4921 gctaataatt tttatcagca ttgggaatcc agagctggta tagcaaaaac atcagattta
     4981 cttacagcag gagatgtaat ttcagtagac tttaccaaag atggagatat agaccatact
     5041 gcaattataa cttccaatat taacaataca aatgaatcta aattgttgac acaacacagt
     5101 attgatagaa gtgaattttt acctacagga gattattttt ctttagcaaa tctatatagt
     5161 caagattata aaatatatgg atatgaaatg gataaagcac caataaaata aaatacgaaa
     5221 ggagcttttg ctcctttcat tttttattta t
//
LOCUS       DAOMTA010000100         5225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_339_length_5225_cov_2.224565, whole genome shotgun sequence.
ACCESSION   DAOMTA010000100 DAOMTA010000000
VERSION     DAOMTA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_339_length_5225_cov_2.224565"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..2486
                     /locus_tag="PLK68_04030"
     CDS             3..2486
                     /locus_tag="PLK68_04030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HRM89611.1"
                     /translation="MATLALLMSAGYVAQDYQVTPTYAETTKIGDSAQLISTATTWKY
                     LDNNVDPGTETDRYAWTKADYNDSEWKSEAGKFGAKKGKLEDLGDGFVPTVLLNQYIN
                     GVNGDDIPAFFFRTTVNISNLDDFSSLSGKLYYDDAAIVYINGVKVASFDEPEGGFES
                     NMSYGGSNASNPKEGVISLTKEQLKDVIKTGQNTIAVELHQGRASSSDIYFEFNNLQV
                     DYGQEETTVEQKALNLTIGEDETKMNLTWYANTNTSGTVQLAKAGAMINGEFPSQFTT
                     VEATNNQANDKGFYYNQATLANLEENTKYVYRVVNGDQVSKIYDFTTKDFDGSYNFIF
                     AGDPQIGASGSASKDTEGWDKTLSDSINKFNPNFILSAGDQVNTASDENQYSGYLDHE
                     ELTSVPQATTIGNHDSSSNAYTQHFNLPNETAKGETAAGTDYWYVYNNTLFMNINTNN
                     TSTAEHKAFMKEAIKENQDVRWKVVVFHHSVYSVASHSVESSILKRREELTPVFDDLG
                     IDVVLMGHDHVYVRSNMMKGMKVSQETKDLTSVTDPEGILYLTANSASGSKYYDIKTN
                     ISTDFVAKMDQSKQRSISNIEVSENSFKVTTYLYNSNDNQWSTLDEFTINKSVETNNQ
                     EITLVPEETANDIRVVAPVGTVEKNSTLSAVEVNEGDLYQGIKNTLNTILGSDKDFKV
                     FDLSLIKNGNIINPLGKVQLSLALPEGYDASKLLIYQVQDSQNNDITLNPITYTIKDG
                     RIIIETASLGQFVLVNNVENKKPDTGNGSNTNLPTVKPSNTNNSTTTTNNKVVTGDNS
                     NLIAWGTLLFTALGCSLVAYKSKKEEKLK"
     gene            2533..3696
                     /locus_tag="PLK68_04035"
     CDS             2533..3696
                     /locus_tag="PLK68_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM89612.1"
                     /translation="MKIFNELKENRVVIIIMLITIIAAGFFNQYLAKDYSRVNNDSTD
                     FVSGKIVEITSSNLEYDQDLKINLGKQVVVVEILEGKSAGKRVEIDNYLTAAHNVEVA
                     IGSKVIISADEPDGIDSYYTVYNFDRGLGMIIFTCVLLLVIIAIGRGKGVKAILGLAY
                     TLYLVIFLLLPTVFSGYSPVLMSIICVALSTIVTLMLLNGASKKTYSAIVATVLGVVL
                     SAGGFYLMSLVLKVNGFSVDEAESLVLINQATGLSIKDILFAGILISSLGAIMDVGMS
                     IVSALSELFHHQPNLTQKQIFDSGIEIGKDMIGTMTNTLILAFTGSAFVSLLVLFSYN
                     VDIKQLLSSNYIAIEFAQGIAGTLGIVLTVPIASFISAWALTNKKSNNNFFKS"
     gene            3761..5203
                     /locus_tag="PLK68_04040"
     CDS             3761..5203
                     /locus_tag="PLK68_04040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM89613.1"
                     /translation="MSFPKGFLWGGATAANQLEGGWNEGGKGISCPDICTGGSHTQSK
                     RITPTLEEGTFYPSHMAIDHYHRFKEDIALFAKMGFKVYRFSIAWTRIFPNGDETTPN
                     EEGLKFYEDLIDECLKYNIEPLITISHYEVPFGLTKKCNAWASREMIDYYMNYCKTIF
                     ERYKGKVKYWLTFNEINSATMPMGAFLSQGILNEQEKSTDFTNQVDDPQLRFQGLHHQ
                     FIASAKAVQLAHEIDPDYKVGNMMIYATSYPLTCDPDDIIKNQQQNHIMNYFCSDVQC
                     RGYYPTYMKRYFKENNIEVTMEPGDEEILKAGPVDFYTFSYYMSVCQTADPTKGAGEG
                     NILGGVANPYLKASDWGWQIDPKGLRYSLNEIYDRYQIPVMVVENGLGAYDEIEADGS
                     INDDYRIDYLRSHIEQMHEAILDGVDLIGYTPWGCIDLVSASTGEYAKRYGFIYVERY
                     DDGTGDFSRREKKSFNWYKKVIETNGEELG"
BASE COUNT         1882 a          683 c         1000 g         1660 t
ORIGIN      
        1 caatggcgac attagcattg ttgatgtcgg ctggatatgt agcgcaagat tatcaagtta
       61 caccaactta tgctgaaaca acaaaaattg gagattcagc gcaattgatt agtacggcaa
      121 caacatggaa atatttagac aataatgttg atccgggaac tgaaactgac cgttatgcct
      181 ggacaaaagc agattataac gattcagagt ggaaaagtga agctggaaaa tttggtgcaa
      241 aaaaaggaaa attagaagat ttaggcgatg gttttgtacc aacagtttta ttaaatcaat
      301 atattaatgg ggttaatgga gatgatattc cagcattctt ctttagaact acagttaata
      361 ttagtaattt agatgatttc agtagtctaa gtgggaaact ctattatgat gatgcagcaa
      421 ttgtttatat caacggtgtt aaggtagcat cgtttgatga accagagggt ggctttgaat
      481 caaacatgtc ttatggcggt tcaaatgcta gtaatcctaa agaaggagtt atttcactaa
      541 caaaagaaca gttaaaagat gttattaaga ctggtcaaaa tacgattgct gtagaattac
      601 atcagggtcg tgcgagcagt tcagatatct attttgagtt taataatctt caagttgatt
      661 acggccaaga agaaacaaca gttgaacaaa aggcgcttaa tttaactatc ggtgaagatg
      721 aaacaaaaat gaatcttacc tggtacgcta atactaatac ttcgggaact gttcagctgg
      781 ctaaagcagg agctatgatc aatggtgaat tcccaagtca atttacaacg gttgaagcta
      841 cgaataatca ggcaaacgat aagggatttt actataacca agcaacattg gctaacttag
      901 aagaaaatac taaatatgta tatcgcgttg ttaatggtga tcaagtgagc aaaatttatg
      961 atttcacaac taaagatttt gatggaagtt ataacttcat ctttgcaggt gatccacaaa
     1021 ttggagcaag tggcagtgct tcaaaagata cagaaggatg ggataaaaca ttaagtgatt
     1081 caattaataa gtttaatcca aacttcattc tttcagctgg tgaccaagtt aatactgcaa
     1141 gtgacgagaa tcaatacagt ggctacttag atcatgaaga attaacaagt gtacctcagg
     1201 caacaacaat cggaaatcat gatagtagtt caaatgctta tacacaacat ttcaatttgc
     1261 caaatgaaac tgctaaaggg gaaactgcag caggaactga ttattggtat gtttataata
     1321 atactttatt tatgaatatc aatacaaata atacaagtac cgcagaacac aaagcattta
     1381 tgaaagaagc aattaaggaa aatcaagatg ttcgttggaa agtagttgtt ttccatcact
     1441 cagtttattc agttgcttct cattcagtag agagttctat tttaaaacgt cgtgaagaat
     1501 taacacctgt atttgatgat ttaggtattg atgttgtttt aatgggacat gaccatgttt
     1561 atgtacgtag taatatgatg aaaggaatga aagttagtca agaaacaaag gatttaacta
     1621 gtgtaacaga tcctgaagga atcttatatt taactgccaa ttcagcaagt ggcagcaagt
     1681 attatgatat caagactaat attagtactg actttgttgc taaaatggat caatcaaaac
     1741 aacgttcaat ttcaaatatt gaagttagtg aaaatagttt taaagtaaca acttatttat
     1801 acaacagtaa tgataatcaa tggtcaactt tagatgaatt cactattaat aaatcagttg
     1861 aaacaaacaa tcaagaaatt acattagtac cagaagaaac agcaaatgat attagagtag
     1921 ttgctccagt aggaacagtt gaaaaaaaca gtactttatc agcagttgaa gtgaatgagg
     1981 gtgacttata tcaaggaatt aaaaatacct taaatactat tttaggtagt gataaggatt
     2041 ttaaagtatt tgatctatca ctaatcaaaa atggaaatat tataaatcca ttaggaaagg
     2101 ttcaattatc attagcacta ccagagggat atgatgctag taagttattg atctatcaag
     2161 tacaagatag tcaaaacaat gatataacac taaacccgat tacatataca attaaagatg
     2221 gacggattat tattgaaaca gcttcattag gacaatttgt tttagttaac aatgtagaaa
     2281 ataaaaagcc agatacaggt aatggttcaa atactaatct tccgacagtt aagccatcaa
     2341 atacaaataa ttctactaca acgacaaata ataaggttgt aaccggtgat aatagtaatc
     2401 ttattgcttg gggaacatta ttgtttacag ctttgggatg ttcattagtt gcttataaat
     2461 caaaaaaaga agaaaagtta aaataactag tggtaatcat tgattaccac taacttttat
     2521 tagaggtcat ttatgaagat atttaatgaa ttaaaagaga atcgtgttgt tattatcatt
     2581 atgctcatta cgataattgc agctggtttt tttaatcaat atttagctaa ggattattct
     2641 cgggttaata acgattcaac tgattttgtt agcgggaaga tagtagaaat aacgagcagt
     2701 aatcttgaat atgatcaaga tttaaaaata aatcttggaa aacaagtagt tgtggttgag
     2761 attcttgaag gaaaaagtgc aggaaaaaga gtagagatcg ataattatct aacagctgct
     2821 cataatgttg aagttgcgat tggctcgaaa gtgattatct cagctgatga accagatgga
     2881 atagactcgt attatacggt ttataatttt gatcgtggct tagggatgat tatttttact
     2941 tgtgtattat tgcttgttat cattgcaatt gggcgcggaa aaggggttaa agcaatctta
     3001 ggattagctt atacattata tttagtaatt tttctattac tgccaactgt attttcaggt
     3061 tactcaccag tattaatgag tattatctgt gttgctttat caacgattgt tacattaatg
     3121 ttgttaaatg gtgcttctaa aaagacttat agtgcaattg tggcaacagt tttaggggta
     3181 gttttatcgg cagggggatt ttatttaatg tcattagtac taaaagttaa tggttttagt
     3241 gttgatgagg cagaaagcct tgtgttaatt aatcaagcta cgggattatc gattaaagat
     3301 attttatttg ctgggatttt aatttcatca ttaggggcaa ttatggatgt tggaatgtca
     3361 attgtttctg ccttatcaga attatttcat catcagccaa acttgactca aaaacaaatt
     3421 tttgattcag gaatagaaat tggtaaagat atgattggta cgatgacaaa taccttgatt
     3481 ttagcattta ctggtagtgc atttgtttca ttattagtct tattttcata caatgtcgac
     3541 attaaacaac tattaagttc taattatatt gcaatagaat ttgctcaagg aattgctggt
     3601 actttaggaa tcgtattaac tgttccaatt gcttcgttta ttagtgcctg ggcattaact
     3661 aataaaaaga gtaataacaa ttttttcaag agttgaaaaa aattttgtaa tatctgaata
     3721 atcagttaaa atgtacatga cataaacaag gaggtattat atgagttttc caaaaggatt
     3781 tttatggggt ggtgcaactg ctgctaacca attagaaggt ggttggaacg aaggtggaaa
     3841 aggaatttca tgccctgaca tttgtactgg tggaagccat actcaaagta aaagaattac
     3901 accaacatta gaagaaggga ctttctatcc atcacatatg gcaattgatc attatcatcg
     3961 tttcaaagaa gatattgctt tatttgctaa aatgggattt aaagtatatc gtttttcaat
     4021 tgcttggaca agaatttttc ctaatggtga cgaaacaaca ccaaatgaag aaggattaaa
     4081 attttatgaa gatttaattg atgaatgctt aaaatataat attgaaccat tgatcacaat
     4141 ctcacattat gaagtaccgt ttggtttaac aaagaaatgc aatgcttggg catcacgaga
     4201 aatgattgac tattacatga attattgtaa aactattttt gaacgctata aaggtaaagt
     4261 aaaatattgg ttaactttta atgaaattaa tagcgcaacg atgccaatgg gagctttttt
     4321 atcacaagga attttaaatg aacaagaaaa aagcactgat tttacaaatc aggttgatga
     4381 tccccaatta cgttttcaag gattgcatca tcaatttatt gctagtgcta aagccgttca
     4441 attagcccat gaaattgatc ctgattataa agttggtaat atgatgattt atgcaacatc
     4501 atatccatta acttgtgatc ctgatgatat cattaaaaat caacaacaaa atcatatcat
     4561 gaactatttc tgtagtgatg ttcaatgccg tggatattat ccaacataca tgaagcgtta
     4621 ttttaaagaa aataatattg aagtaacgat ggagcctggg gatgaagaaa tattaaaagc
     4681 tggaccagtt gatttctata cattctcata ctatatgtca gtatgtcaaa cagccgatcc
     4741 aactaaaggg gctggtgaag gtaatatttt aggcggtgta gcaaatccat atttaaaagc
     4801 atctgattgg ggatggcaaa ttgatcctaa aggattacgt tattcactaa atgaaatcta
     4861 tgatcgttat caaattccag taatggtagt tgaaaatggt ctaggagcat atgatgaaat
     4921 cgaagcagat ggttcaatta atgatgatta ccggattgat tatttgagaa gtcacattga
     4981 acaaatgcat gaagcaattc tagatggtgt ggatttaatt ggttatacac catggggatg
     5041 tattgatttg gtatctgcat caacaggaga atatgctaag cgttatggtt tcatttatgt
     5101 tgaaagatat gatgatggaa caggtgattt ttcaagaaga gagaaaaaat catttaattg
     5161 gtataaaaaa gttattgaaa ctaatggtga agaattggga taatagatgt gcatagcaca
     5221 tctat
//
LOCUS       DAOMTA010000101         5174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_346_length_5174_cov_2.329947, whole genome shotgun sequence.
ACCESSION   DAOMTA010000101 DAOMTA010000000
VERSION     DAOMTA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_346_length_5174_cov_2.329947"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1539
                     /gene="thrS"
                     /locus_tag="PLK68_04045"
                     /pseudo
     CDS             1..1539
                     /gene="thrS"
                     /locus_tag="PLK68_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537936.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            1703..>2442
                     /locus_tag="PLK68_04050"
     CDS             1703..>2442
                     /locus_tag="PLK68_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89614.1"
                     /translation="MKYYLAMDVGGTSIKYGVVNDQGKIINTDKIVTPDSLEKMYQAM
                     GEIYHNCNYEVAGIALSMPGAVNSEVGNIEGASALDYIHGPNIKEDLQKRFNTKVSIE
                     NDANCAALAEVWKGSGSDVDDCMFIVSGTGIGGAVVKDRMIHKGKHLHGGEFGYMVAL
                     NDLDNDSYISWSTAGSTVATVKGVAKELGVDYQTLDGKVIFDQAQDNPIYQKYVDRYY
                     SVLAMGIYNLQYVYDPEKIIIGGAISVRP"
     assembly_gap    2443..2477
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2652..3329
                     /locus_tag="PLK68_04055"
     CDS             2652..3329
                     /locus_tag="PLK68_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRM89615.1"
                     /translation="MKREAPKINTQLRSHTIMVPECIRNASGIVINGKRIKSLLFSTD
                     VAVISNCNADAVIAVYPFTPTMQITNSIIDVAQRPVFAGVGGGTTAGPRVREIALDAE
                     LHGATAVVLNAPTKTEFIQELSDYVDIPVVLSIVSLDENLEERMLHSGATIVNVSGGK
                     NTVAIVKALREISQDFPIIATGGPNLETIQAVIEAGANAITYTPPSNGEIFKAMMEDY
                     RQRCQHQ"
     gene            3412..4290
                     /locus_tag="PLK68_04060"
     CDS             3412..4290
                     /locus_tag="PLK68_04060"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRM89616.1"
                     /translation="MKQYLDMCKYVLENGENREDRTGTGTRSVFGYQTRYDLRDGFPL
                     MTTKKMFLRPIAEELLWFIKGDTNIKYLVDRNVKIWNEWPYEDFKKSPDFNGETIEEF
                     VEKIKTDDEFAKKHGDLGPVYGAQWRDFNYEGVDQLAKLVDSLTNNPFSRRHIICAWN
                     PAQVDNMALPPCHAFLQFYVSADKKYLSCQLYQRSADIFLGVPFNIASYALMTEMLAR
                     TCGYEAKEFIHTIGDAHIYKDHFEVVKQQIAREPLPKCKLVLNPDVKSIFDYKIEDIK
                     IEDYQSHGKLVGKVSV"
     gene            4290..4760
                     /locus_tag="PLK68_04065"
     CDS             4290..4760
                     /locus_tag="PLK68_04065"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRM89617.1"
                     /translation="MINIIVATDEDLLIGKKDSRNGMPWNVPEDLQHFKATTLNKTIL
                     MGLTTYQAIGRPLPNRKTIVVSFEPFEDERVEVRSSLEEVIEEYRSSGEDLFISGGAS
                     IYKQCLPIADQLLISRIPGKHEGETYFPNFDEYGYKLVAQKPFETFTLETYKRG"
     gene            4765..>5174
                     /locus_tag="PLK68_04070"
     CDS             4765..>5174
                     /locus_tag="PLK68_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM89618.1"
                     /translation="MKRIALIVIRVIWRLPYWWGYKVNKYKHIDKYSADERYGFIHQV
                     VAIVTRKARVELECYGVENLPKKSGYFVAPNHQGLFDPLAIFQTHSRPIRAIVKKELA
                     SVILVKDVIKMLEFIPMDRSNVRESAKIIKYVAN"
BASE COUNT         1798 a          655 c         1074 g         1612 t
ORIGIN      
        1 aaaagaaatg aaaaagattt gtaaagatgg caaacgtatt actcgccaag aaatttctaa
       61 agaagaagcc ttagaaatgt tcaaagatga tgaatataaa atagatttga tcaataattt
      121 tgaaaatgat acaactatta gttgttatcg tcaaggagat tttgttgatt tatgtagagg
      181 tccgcatgtt gaaacagtta aagcttgtaa gaactttaaa ttgactaaac attcaggtgc
      241 gtattggaag ggtgacaaag aaaataaggt actacaaaga atttatggtg tttgttttga
      301 aacttctgaa gacttagcta aacatcttga attattggaa gaggcaaaac ggcgggatca
      361 taagaagcta ggtaaagaac taggattatt tatgatgagt gaatacgctc ctgggatgcc
      421 atttttctta ccgaaaggaa tgattttaag aaatacttta gaacaattct ggtacgaaga
      481 acatgctaaa gaaggatatg aattcattaa aacaccaatt atgatgtcac gtgaattatg
      541 ggaaactagt ggtcattggg aaaattataa agaagatata ctagtatggt tgatgaccgt
      601 gaatttgcaa ttaagcctat gaactgtcca ggaagtttgt tagtatataa aaattcctta
      661 cattcataca aagatttacc attaagaatg ggtgaactag gtcaagttca tcgccatgaa
      721 gctagtggag ccttgaatgg tttatttaga gtacgtactt ttactcaaga tgatgcacat
      781 atctatatga cacctgatca aattgaaggt gaaattataa ggttgattaa tttcattgat
      841 cgtgtttatg gaagcttaaa tttaagttat gaaattgaat tatctacccg tcccgagaag
      901 aaatatattg gtgatttagc aatctgggaa aaaagtgaag ctgctttagc tgccgcttgt
      961 aaagcagcag gtaaggatta taaagtaaat cctggagatg gtgctttcta tggaccaaaa
     1021 ctcgatttcc atgttaaaga ttcattaggg agagtttggc aatgcggaac aattcaatta
     1081 gacatgaatt taccagagag attcgatatt acttatattg atgataaagg tgaaaaagtt
     1141 cgtccagtta tgctgcatcg tgttatcttt ggatcgattg aaagatttat tggaatttta
     1201 attgagcatt ttgctggtgt gttcccatta tggttggcac ctgttcaagt taaagtattg
     1261 ccggttaata atgagtatca tttagactat gctaaagaag taactgagtt attaaaggat
     1321 aaaggcttta aagttgaact ggatgctcgt gaagaaaaat tgggttatcg aattcgtgag
     1381 ggacaaatgg aaaaggtacc ttatttatta gtattaggta ataatgaacg tgatgaaaaa
     1441 actgttacat atcgtaaaca tggagagcaa aaacaaatta ctgttccatt tgatgatttt
     1501 gtcgcaatgc taaatcagca gattgttgat aaaaaatagt aaacacttag caaattaaat
     1561 ttgctaagtg ttctttttgg tttgataaac ttctatttta tagatgcgta attaaaatta
     1621 ttagaataaa tttacattat ttatatttcg aagaaagata tagatgtgat ataattaaaa
     1681 tattgaaaag agggataaaa agatgaaata ttatttagca atggatgttg gtggaacgtc
     1741 aataaaatat ggtgttgtca atgatcaggg aaaaatcatt aatactgata aaatcgtaac
     1801 gcccgattca ctagaaaaaa tgtatcaggc aatgggtgag atttatcata attgtaatta
     1861 tgaagtagcg ggaattgcat taagcatgcc aggtgccgtt aacagtgaag ttgggaatat
     1921 tgaaggagca agtgctttag attatatcca tgggccgaat atcaaagagg atctacaaaa
     1981 acgcttcaac acaaaagttt caattgaaaa cgacgctaac tgtgcagcat tagcagaagt
     2041 ttggaaagga tctggaagtg atgtggatga ctgtatgttc attgttagtg gcactgggat
     2101 aggtggtgct gttgttaagg atcgaatgat tcataaagga aaacatcttc atggcggtga
     2161 atttggctat atggtggcct taaatgatct tgataatgat tcctatattt cttggtctac
     2221 tgcaggatct acagttgcaa cagtaaaagg ggttgctaaa gaattgggag ttgattatca
     2281 aactttagat ggtaaagtga tttttgatca agcacaagat aaccctattt atcaaaaata
     2341 tgttgatcgg tactatagtg tcttagcaat ggggatatat aatcttcaat atgtctatga
     2401 tcctgaaaaa attattattg gtggagcaat tagtgtacga ccnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnaaa aatatatgat agtattcctg ttgcaaaaat tcatcctaaa
     2521 gtagttaaat gccgttttgg aaatgaagca aatttaattg gagcagttta tcactttatt
     2581 catagctagt cattaagaat tcatattatt gtattaaaat attattaaat aaaacaaggg
     2641 gaggtaaaaa aatgaaaaga gaagcaccaa aaatcaacac acagctaaga agccatacaa
     2701 ttatggtacc agagtgtatc cgcaatgcca gtggaatagt tattaatggc aaaagaatta
     2761 aatcattatt attctcaaca gatgttgcag taatatccaa ttgtaatgct gatgctgtaa
     2821 tcgcggttta tccatttacc ccaactatgc aaattactaa ttcaattatc gatgtagcac
     2881 aacgtcctgt ttttgcgggc gttggcggtg gaacaacagc aggacctcga gtacgggaga
     2941 ttgctttgga tgcagaatta catggtgcaa ctgcagttgt tttaaatgca cctacaaaaa
     3001 ctgaatttat tcaagaatta tctgattatg ttgatattcc agtagtatta tcaatcgttt
     3061 ctttagatga aaatcttgag gaacgcatgt tacattctgg agcgacaatt gttaatgttt
     3121 cgggtggtaa gaatactgtt gcaatcgtca aagcattaag agagatttct caagattttc
     3181 caatcattgc taccggtgga cctaatcttg aaacaattca ggcagttatt gaagcaggtg
     3241 caaatgccat tacatataca ccgccttcaa atggtgaaat ttttaaagcg atgatggagg
     3301 actatcgaca gcgttgccaa catcaatgat ttgttttggg tagattgata tctacctttt
     3361 ttgttgttca aactggtatt ttattgtaca atagttgctg aggtgtaaaa aatgaaacaa
     3421 tatctagata tgtgtaaata tgtattagaa aacggagaaa atcgcgagga tcgaacagga
     3481 acaggaacac gtagtgtttt tggatatcaa actcgttatg atttgagaga tggtttcccg
     3541 ttgatgacaa ctaaaaaaat gtttttgcgg ccaattgctg aagaattatt gtggtttatt
     3601 aagggtgata caaatatcaa gtatttagtt gatcgcaatg tcaaaatatg gaatgaatgg
     3661 ccatatgaag attttaagaa atcacctgat tttaatggtg aaacaattga agagtttgta
     3721 gaaaaaatta agacagatga tgaatttgca aaaaaacatg gtgatttagg gccggtatat
     3781 ggcgctcaat ggcgtgattt taattatgaa ggggttgatc agttagcaaa attagttgat
     3841 agtctgacta acaatccttt ttcaagaaga catattatat gtgcatggaa tccagcacaa
     3901 gtagataata tggcgctgcc gccatgccat gcatttttac agttttatgt tagtgcagat
     3961 aaaaaatatc tgtcttgtca actatatcaa cgtagtgcgg atattttttt aggggtaccg
     4021 tttaatattg cttcgtatgc tttaatgacg gaaatgttag cgagaacttg tggatatgaa
     4081 gcaaaagaat ttatccatac gattggtgat gctcatattt ataaagatca ttttgaagta
     4141 gttaaacaac aaattgcaag agaaccatta ccaaaatgta aattagtatt aaatccagat
     4201 gtgaaatcaa tttttgatta taagattgaa gatattaaga tagaagatta ccaaagtcat
     4261 ggtaaattag ttggaaaggt aagtgtataa tgataaatat aattgttgca acagatgagg
     4321 atctgttaat tggaaagaag gattcacgaa atggaatgcc ttggaatgtt ccggaagatt
     4381 tacaacattt taaggcaacg actttaaata aaacaatttt gatgggattg actacatatc
     4441 aggcaatcgg acgtcctttg cctaatcgta aaactattgt tgttagtttt gaaccgtttg
     4501 aagatgaacg agtagaagtg agaagctcat tagaagaagt aatagaagaa taccgtagta
     4561 gtggtgaaga tttatttatt tcgggtgggg cttcaattta taaacagtgt ttaccaattg
     4621 cagaccagtt gttaatctca aggattcctg gaaaacatga aggtgaaaca tattttccta
     4681 attttgatga atacggttat aaattggttg ctcaaaaacc atttgagaca tttacattag
     4741 aaacatataa gcgaggataa ttaaatgaag agaattgcat tgattgttat tcgtgttatt
     4801 tggcggttac catattggtg gggatataaa gtcaataagt ataaacatat tgataaatat
     4861 tctgctgatg aacgctatgg gtttatacat caagttgtag caattgtaac acgcaaagca
     4921 agggtagagc tagagtgtta tggggtagaa aatttaccca agaaatctgg ttattttgtt
     4981 gcccctaatc atcaaggatt gtttgacccg ctagcaattt ttcaaaccca tagtcgtcca
     5041 attcgagcaa tcgtaaaaaa agaattggca tcagtcattt tagtaaagga tgttattaag
     5101 atgctggaat ttattccgat ggatcgcagt aatgttcgag aaagtgcaaa aatcattaaa
     5161 tatgttgcta atga
//
LOCUS       DAOMTA010000102         5108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_359_length_5108_cov_2.365328, whole genome shotgun sequence.
ACCESSION   DAOMTA010000102 DAOMTA010000000
VERSION     DAOMTA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_5108_cov_2.365328"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLK68_04075"
     CDS             <1..653
                     /locus_tag="PLK68_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRM89619.1"
                     /translation="ENINYIQIHPTTLYTTKPGRSFLISESVRGEGAHLLNPDMERFV
                     DELLPRDVVSNAIKKEMDKYNVPHVYLSVTHMDPKQIKRRFPHIYDQCLAEGYDMFTD
                     PVPVVPAQHYLMGGIKSDTYGQTSMNNLFAVGETACNGVHGANRLASNSLLESLVFAK
                     RAAKVIADGIDKISLYEKVVDLDGYDKEKLKKENKEIIMNEIKRKDGEFYDKWCKS"
     gene            631..1485
                     /gene="nadC"
                     /locus_tag="PLK68_04080"
     CDS             631..1485
                     /gene="nadC"
                     /locus_tag="PLK68_04080"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRM89620.1"
                     /translation="MINGVNLKVNIDDYILSALKEDITSDDVTTNAVMPEAKLGHVDL
                     ICKQDGIVCGLEVFERTFKLLDENCKFTTKYKDGDVIKNGDYIGEVIGDIRVLLSGER
                     VALNYLQRMSGIATYTSESVAILKGSKTKLLDTRKTTPNNRIFEKYAVKIGGGTNHRY
                     NLSDGVLIKDNHIGAAGSIAKAVAMARDYAPFVRKIEVEVETLEQLQEALEAKADIIM
                     LDNMDNATMKEAVAMIGDKAQSECSGNVTKERLMEIAEIGVDFVSSGAITYAAPILDF
                     SMKNLRPY"
     gene            1623..2990
                     /locus_tag="PLK68_04085"
     CDS             1623..2990
                     /locus_tag="PLK68_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89621.1"
                     /translation="MENESYWQHTITLPKYPQINKNCNYDVVIVGGGISGVSLAYRLN
                     NSNLKVALFESDTLGSKTTGHTTAKVTYLHGAVYADIYQVYGRSKAKQYLESNYEAYQ
                     DIKKIIEMEQIECDFKENIAYVGASDTTNAKKLDCQIRLFKSWGFEVLENRLKNCQIS
                     MGLKQQAIFHPLKYLKGLLAQCKHIDIYEHSLVTGSMHQDGLVCLEVNGFKVQARQVV
                     WMTRYPPNLQHGYFFRIIQEKEHVIFQEGQSNGNSILDLSTNYSKRYLDEQHILMIKR
                     IDDLNQVYWYAQDGKPLRKIPYIGKMNEQEYVAYAYNKWGMTLSHVASKLIYDLIIND
                     ESKYASLYQPSYGNYLKSGADMLKLVKNNYHGMIKNRLVSSKKLKLKKQQGKVIRHQG
                     RLLAVYKDAQERIFYFSPYCPHLKCVVQYNEIDNTWNCPCHGSIFDCHGKLVSGPATK
                     DLKQY"
     gene            complement(3004..3627)
                     /locus_tag="PLK68_04090"
     CDS             complement(3004..3627)
                     /locus_tag="PLK68_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89622.1"
                     /translation="MMEPIYRCGYQIPSLIKLVFKLICFITIPFLITMMVKNLIVTNG
                     IAVDHSFSFIIYPISLLITLGFVYSYRNSSIYLFYNNFMLYKEKLNLHDRMKLFFMKD
                     DLNELENYLSKRNYANLEELNIIVRKVSFMRTNDIGIEIKFKLIFNDQKNFIITVTED
                     TREAYIQFLEPFIKYHVKINDPSNLIDGLKQPLRLTEYFIRNKSMED"
     gene            complement(3608..4258)
                     /locus_tag="PLK68_04095"
     CDS             complement(3608..4258)
                     /locus_tag="PLK68_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRM89623.1"
                     /translation="MKINYDLKFKEELTKIKNTRPRLLLHVCCGPCSGNVIRELADIF
                     EITIYYSNSNIYPNAEYHRRYQELLGFIKQFNQDYHQNITVIEQPYLPKEYLNKLSRY
                     KDEPEGGKRCYLCYQKRMNDAFQYSCEHNFDYWTTVLSVSPHKNSQWINEIGASFAQN
                     KTKFLFSDFKKNNGYLKSVRFADSYHLYRQSYCGCVYSYQDMLKRKKDVEDDGTNL"
     gene            complement(4303..>4539)
                     /locus_tag="PLK68_04100"
     CDS             complement(4303..>4539)
                     /locus_tag="PLK68_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRM89624.1"
                     /translation="EVLANSEAVIAYQLDYDHEIAKMFTALSKKHVTFTACNNALAAN
                     HLTAADIYQFITIVPAGVVELAQKQTEGFAYIKP"
     assembly_gap    4540..4556
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4665..>5108)
                     /locus_tag="PLK68_04105"
     CDS             complement(4665..>5108)
                     /locus_tag="PLK68_04105"
                     /inference="COORDINATES: protein motif:HMM:NF039470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HRM89625.1"
                     /translation="YNVNLKEIYIFFFYYDRIKSRGSENMFSYHKEDFIIRQIQSAAR
                     ALAKLFFNSETVSYTIKDESNYTQSDLIYLKIKELMEQHKYLEAQTLLLEHLDSDDLQ
                     YLKLILYFYDQLDQMNDEQLAKYYLERQIITNNFIDATSKYNCLI"
BASE COUNT         1807 a          644 c          887 g         1753 t
ORIGIN      
        1 tggagaatat taattatata caaattcatc caactacttt atatacaact aaaccgggta
       61 gaagtttttt gattagtgaa tcggttcgtg gtgagggggc tcatttatta aatcctgata
      121 tggagcgttt tgttgatgaa ttattgcctc gtgatgttgt aagtaatgcg atcaaaaaag
      181 agatggataa atataatgta ccccatgttt atttatctgt tacacatatg gatccaaagc
      241 aaatcaaaag gagatttcct cacatatatg atcaatgtct tgctgagggt tatgatatgt
      301 ttacggatcc agttcctgtt gttccggctc aacattattt aatgggagga atcaagagtg
      361 atacttacgg acaaacttca atgaataatc tttttgcagt tggagagact gcttgtaatg
      421 gggttcatgg cgctaatcgt cttgcaagta attctttatt agagagttta gtttttgcta
      481 aacgagcagc caaagttatt gctgatggta ttgacaagat tagcctttac gaaaaagtag
      541 tagatttaga tggctatgat aaagaaaagc taaaaaaaga aaataaagaa ataattatga
      601 atgaaataaa aagaaaagat ggtgaattct atgataaatg gtgtaaatct taaggtaaac
      661 attgatgatt acatcttaag tgcgttgaag gaagatatca cgagtgatga tgttacaaca
      721 aatgcagtaa tgccagaagc taaattagga catgttgatt tgatttgtaa acaggatggg
      781 atcgtttgtg gtttagaggt attcgaacgg acttttaaat tattagatga aaattgtaag
      841 tttacaacaa aatataaaga tggtgatgta atcaaaaatg gtgattatat tggtgaagta
      901 attggtgata ttagagtttt attatctgga gagcgtgttg ctttaaatta tttacaacga
      961 atgagtggga ttgctactta tacaagtgaa tcagtagcaa tcttaaaagg atctaagaca
     1021 aaattattag atacacgtaa aactacacca aacaatcgaa ttttcgaaaa atatgcggtt
     1081 aaaataggtg gtggaactaa tcatcgttat aatttatcag atggagtatt aattaaagat
     1141 aatcatattg gagcagctgg aagcattgct aaagcagttg caatggctcg tgactatgcc
     1201 ccatttgttc gtaaaattga agttgaagtt gaaactttag aacaacttca agaagcactt
     1261 gaggctaaag cagatatcat tatgctggat aatatggata atgcaactat gaaggaagca
     1321 gtggcaatga ttggtgataa agctcagagt gaatgttctg gaaatgttac taaagagcgc
     1381 ttaatggaga ttgcagagat tggggttgat ttcgtatctt caggtgcgat tacttatgct
     1441 gctccaattc tagatttttc aatgaaaaat ttacgaccat attaaataga taaagttaat
     1501 tgggctggtt taaggaataa tcttaaatca gcttttttag ttaataaaaa ttattttatg
     1561 attaattaaa aaaagtgtag tttattgact aattaaaagg gcatacttaa tctggtgata
     1621 aaatggaaaa tgaatcatat tggcagcata cgattacgct gcctaaatac ccgcagataa
     1681 acaaaaattg taactatgat gttgttattg ttggcggtgg gattagtggt gtaagtttag
     1741 cttatcgtct taataacagt aatttaaaag tagcattatt tgaaagtgac acacttggta
     1801 gtaaaacaac tgggcatact acagctaagg ttacttattt gcatggtgct gtttatgctg
     1861 atatttatca ggtctatggc cggagtaaag caaaacagta tttagaatca aattatgaag
     1921 cttatcagga tattaaaaaa ataattgaaa tggaacaaat cgagtgtgat tttaaagaaa
     1981 atattgctta tgtaggagca agtgatacaa caaatgcaaa gaaacttgac tgtcaaatca
     2041 gattatttaa atcttgggga tttgaggttt tagaaaatcg attgaagaat tgtcagattt
     2101 ctatgggact taaacagcag gctatttttc atccattaaa atacctaaaa ggattactag
     2161 cgcaatgtaa gcatattgac atttatgaac attcattagt aacaggcagc atgcatcaag
     2221 atggattggt atgtttggaa gttaatgggt ttaaagttca ggcaagacag gtagtttgga
     2281 tgactcgcta tccccctaat ttacaacatg gttatttttt tagaattatt caagaaaaag
     2341 aacatgtcat ttttcaagaa ggacagagta atggtaatag tattttagat ttatcgacta
     2401 attattctaa acgatattta gatgaacagc atattttaat gataaaacga attgatgatc
     2461 ttaatcaagt ttactggtat gcccaagatg gaaagccgtt aagaaaaata ccttatattg
     2521 gtaaaatgaa tgaacaagaa tatgtggcct atgcttataa taagtggggg atgacgttaa
     2581 gtcatgttgc tagtaaatta atttatgact taattattaa tgatgaaagt aaatatgcct
     2641 ctctatatca gcctagttat ggcaattatt taaaatctgg ggcggatatg ctgaaactag
     2701 ttaaaaataa ttatcatggg atgataaaaa atcgtttagt ttctagtaaa aaattaaagt
     2761 taaagaagca gcagggtaaa gttattcgac atcaaggacg attattagca gtttataaag
     2821 atgctcagga acgaatattt tatttctcgc catattgtcc gcatttaaaa tgtgtggtgc
     2881 agtataatga gattgataat acatggaatt gtccatgtca tggttctatc tttgattgtc
     2941 atggaaaact tgtaagtgga ccggcaacta aagatttaaa acaatattga aaaagcatgg
     3001 agtttaatct tccatgcttt tattacggat aaaatattca gttaatctaa gtggctgttt
     3061 taaaccgtca attagattac taggatcatt gattttaaca tgatatttaa taaaaggctc
     3121 taaaaattgt atataggctt ctcttgtatc ttcagtgaca gtaataatga aatttttttg
     3181 atcgttaaaa attagtttaa actttatttc tattccgata tcattagttc gcataaaaga
     3241 aacttttcta actataatat ttagttcttc aagattagca taattacgtt tagataagta
     3301 attctctaat tcattaagat catctttcat aaaaaataat ttcatccggt catgaagatt
     3361 tagtttttct ttgtataaca taaaattatt ataaaataaa tagatactag aattacgata
     3421 actataaaca aagcctagag taattaataa ggaaattgga taaataataa aactaaatga
     3481 gtgatctact gctattccgt tagtcacaat taaattctta accatcatgg taattaaaaa
     3541 aggaatagta ataaaacaaa ttaacttaaa tactaatttg attaaagagg gaatttgata
     3601 gccgcatcta taaattggtt ccatcatctt caacatcctt ttttcgtttt aacatatctt
     3661 gataagaata tacgcagcca caataacttt gacgataaag atgatagctg tcagcaaatc
     3721 ttaccgattt taaataacca ttatttttct taaaatcact aaataagaat tttgttttat
     3781 tttgagcaaa acttgctcca atttcattga tccattgact atttttatga ggtgaaacac
     3841 tcaatacagt tgtccaataa tcaaaattgt gttcacagct atattgaaag gcatcattca
     3901 tccttttttg ataacaaaga taacaacgtt tccctccttc tggttcatct ttgtatctac
     3961 taagcttatt taaatattct tttggtaaat aaggttgttc aataactgtg atattttgat
     4021 gataatcttg attaaactgt ttaataaagc cgagtaattc ttgataacga cgatgatatt
     4081 cagcatttgg ataaatatta gaatttgaat aataaatcgt aatttcaaag atatcagcta
     4141 gttctctaat tacattgccg ctacaaggac cacaacaaac atgtagtaag agacgtggtc
     4201 gcgtattttt tatttttgtt aattcttctt taaattttaa gtcataattt attttcataa
     4261 attgattata gcatagttta ttcttgaacg tataaataaa ttttatggtt taatatatgc
     4321 aaaaccttca gtttgttttt gtgctaattc aacaacacca gctggaacga tcgtaataaa
     4381 ttgataaata tcagcagcag tgaggtgatt agctgctaaa gcattgttac atgctgtaaa
     4441 agttacgtgt ttttttgata atgcggtaaa catttttgct atttcatgat cataatcgag
     4501 ctgataggcg ataactgcct cactgtttgc aagaacttcn nnnnnnnnnn nnnnnnattt
     4561 tggacttgat aataattgct catattagta acattgccta aacaaagttg ccattttgtt
     4621 aattcatcaa catgaaagat aattttcatc atatactcct ttaattaaat taaacaattg
     4681 tatttactgg tagcatctat aaaattgtta gtgattattt ggcgctctaa ataatattta
     4741 gctagttgtt cgtcattcat ttgatctaat tgatcataga aataaagaat taattttaaa
     4801 tattgtagat catctgagtc gagatgctca agtaataatg tttgggcttc taaatattta
     4861 tgttgctcca ttagttcttt gatttttaaa taaattaaat cgctttgagt ataattagac
     4921 tcatctttaa tagtataact tacagtttcg ctattaaaaa ataatttagc taaagctctg
     4981 gcagcacttt gaatttgacg gataataaaa tcttctttat gatatgaaaa catgttctca
     5041 cttcctctac ttttaattct atcataataa aagaaaaaaa tgtaaatctc tttaagattt
     5101 acattata
//
LOCUS       DAOMTA010000103         5107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_360_length_5107_cov_2.169240, whole genome shotgun sequence.
ACCESSION   DAOMTA010000103 DAOMTA010000000
VERSION     DAOMTA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_5107_cov_2.169240"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLK68_04110"
     CDS             <1..783
                     /locus_tag="PLK68_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRM89626.1"
                     /translation="NYIIDGIFTAIPQQHKDSLKRRIRIPVPIFISALVPKVMMDYIN
                     ERLAVEPTEKFLDDQNHANVEIFIHVSPDGFGTIGHCDICIDNQVISYGNYDYDSIRL
                     FETIGDGVLFIAPRESYIPFCIETDHKTIFSYGVRLTVKQLASVKREIEKLKENTYPW
                     YPRSYKDRNDCNDYASRLYLRTGASFFKFKRGRYKTYFVLGSNCVKLAEAIMGKAGMD
                     IIDLNGIISPGSYQNYLEKEYQRANGVIISKNVYNQLTIDHK"
     gene            1183..2742
                     /locus_tag="PLK68_04115"
     CDS             1183..2742
                     /locus_tag="PLK68_04115"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRM89627.1"
                     /translation="MAKYIFVTGGVVSGLGKGLTAASLGRILKQRGLKVFMQKLDPYI
                     NVDPGTMSPYQHGEVFVTADGAETDLDLGHYERFIDEELNRNSSITTGRIYSNVISKE
                     RRGDYLGATVQVVPHITNEIKAKIYAAASSSNADIVITEIGGTTGLDLGYDNTLYIHT
                     TLLPYIGASHEVKTKPTQHSVKELRGLGIQPDFIVCRSEKYIEQELKDKISLFCNVPT
                     KNVISNYDVEVLYELPMMLLDQHMDDLVLEHLRIEAPQPNMSEWESLIERVKGLDHEI
                     TIALVGKYTQLPDAYLSVNEALRHAGYYENSVVNIEFVDSEEITKDNVAEKLKTADGI
                     IVPGGFGNRGIEGMIDAIEYARINNLPFFGICLGMQLATIEYARNVCGLKDANSLEFD
                     ELTKNPIINLMSDQSLTDMGGTQRLGDYNCELAAGTHARELYGVDMIQERHRHRYEFN
                     NEYKDALVEHGLTIAGINPERNLVEIVEIKEHPYFVACQFHPEFTSRPNRSQPLFQGL
                     ITAAHKRKYHG"
     gene            2895..4382
                     /locus_tag="PLK68_04120"
     CDS             2895..4382
                     /locus_tag="PLK68_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM89628.1"
                     /translation="MRKIKKLFAMMMTLVMTLGIITTNISAIEELKVTSIVANTYVGD
                     GQREVSSFEITVNDINLVNDLKAEDFDITNNVSSVPYDVATNALADDYLDDGISLEIK
                     GNTIILNVKAFDYTGRYNPDFSKNPWQVTCNKYGVLSFNKDNVTTLKTKTLDDAIRGT
                     FTYAGLTREYALYLPKNSDGTNMKNVPLVVWNHGGGEYAGQLENTLVANRGLTAWVEA
                     GYNTAVLQIQVSNPNYSYGTAFDEDKKSLIDQNNALQAALIKKLIAEGTVDQNRVYVT
                     GASSGGGATMRFIMQYPELFAGAIACCSMDPIVWVHYNYQDSYEQIVENFETAFQGQV
                     YTWDETKEMMVSKQVDTASLVELPIYFTHAQNDTTCNVNSSKAMYEALNNLGAKNNHL
                     TIWSNQEMTDEGIAEGLLHWSWVKVLNHNEEGSPMNWLFKQTNASENIPVEKEESNNS
                     KTETTASEEAKKAVKTGDDSTVELMAMMTGLSLLAGTVVLSKKYY"
     gene            4536..>5107
                     /locus_tag="PLK68_04125"
     CDS             4536..>5107
                     /locus_tag="PLK68_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89629.1"
                     /translation="MIKNITLLDSILDVANCVDINDNNTYIARKVIENCVWLKDMSLE
                     DFLETTNINGSQFKRFYKLYDCNNFSILKERIGLWCDIRKEQVVKQCQWQKQEPLARV
                     IFNLTNYNDFNDFFNVELIDRICKQIYQSKRIIMYGAMGLLNLTHDFQIDMKLFGKDF
                     IRSSMYEDKALVPQKDDFVWLFSMMGRTMN"
BASE COUNT         1820 a          673 c         1002 g         1612 t
ORIGIN      
        1 aattacatta ttgatggaat ctttaccgct attccccagc agcataaaga cagtttaaag
       61 agacggatca gaatacccgt accaatcttt atttctgccc ttgtaccaaa agtgatgatg
      121 gattatataa atgaacggtt agcagttgag ccaacagaga agtttttaga cgatcagaat
      181 cacgcaaatg tagaaatttt tattcatgtt agcccagatg gatttgggac aattggacat
      241 tgcgatatat gtatcgataa tcaagtaata tcttatggaa actatgatta cgattctatt
      301 agattatttg aaaccattgg ggatggtgtt ttgtttattg ctccacgaga aagttatatt
      361 ccattttgca tcgaaactga tcataaaacg atttttagtt atggggttcg tttaacagtt
      421 aaacagttag cgagtgtaaa acgagaaatt gaaaagttaa aagaaaatac ctacccttgg
      481 tatccgcgtt catataaaga taggaatgat tgtaatgact atgccagccg tctttattta
      541 cgaacgggag ctagcttttt taaatttaaa agaggacgct ataaaacata ttttgtttta
      601 gggtctaatt gtgttaagtt agctgaagca attatgggaa aagcaggaat ggatattatt
      661 gatttaaatg gaattatttc tccgggtagt tatcaaaact atttagagaa ggaatatcaa
      721 agggcaaatg gtgttattat ttcaaaaaat gtttataacc aattgacaat tgaccataaa
      781 tgagacataa taatggagta ttatggttat gtcaaagaaa gacaataata ctttgcatag
      841 gtgacccctc tcccatataa tcacctatgc agaaaaggaa caagaaattg ttcctttttt
      901 tatacatttt ttttaaaagt tcataaagtg ttcacatttc tccgatatta ttacaaatgt
      961 cgtcaagaca agataatata taagtatagg tcaccccctt cttccccaag aacgacctat
     1021 acattaaagg aagtattccc caatgcttcc ttttttttat ttgttagtgc aaaaaaattc
     1081 ttgcctagat aataaaaatt tgctagaatg taaaagggca acagtgtgtc ttccagagga
     1141 agacattttt attttgatat aaaacaaaaa ggagaataga aaatggcaaa gtatatattt
     1201 gtaacaggtg gggtcgtatc aggccttggt aaaggtttaa ctgctgcatc gttaggtcga
     1261 attttaaaac agcgagggtt aaaagtgttt atgcaaaaat tagatcctta tatcaatgtt
     1321 gatccaggaa cgatgtcacc atatcagcat ggtgaggttt ttgtaacggc agatggtgct
     1381 gaaaccgatt tagatttggg gcattatgaa aggttcattg atgaagaatt aaatcgtaat
     1441 tcatctatta ctacaggacg aatttatagt aatgttatat ctaaagaacg tcgaggggat
     1501 tatctaggag ctacggtaca agtggtaccg catattacaa atgaaataaa agcaaaaatt
     1561 tatgctgcag catcaagttc taatgcagat attgtaatta ctgaaattgg aggaacgact
     1621 ggcttagatt taggttatga taatactctt tatattcata cgacactatt accttatatt
     1681 ggagcgagtc atgaggttaa aactaaacca acacagcata gtgttaagga gctaagaggt
     1741 ttagggattc agccagattt tattgtatgt cgaagtgaga agtatattga acaagagctt
     1801 aaagataaaa tttcattatt ttgtaatgtt ccaacaaaaa acgtaatttc aaattatgat
     1861 gttgaagtat tatatgaatt accaatgatg ttattggatc agcatatgga tgatcttgtc
     1921 cttgaacatc taagaattga ggccccacaa ccaaatatga gtgagtggga gagtttgatt
     1981 gaacgggtta aaggtttaga tcatgaaata acaattgctt tggtaggaaa gtatactcaa
     2041 ttaccagatg cttatttgtc agtaaatgaa gctttaagac atgcgggata ttatgaaaat
     2101 agtgttgtaa atattgaatt tgttgattct gaggaaataa caaaagataa tgtcgctgaa
     2161 aagttaaaaa cagcagatgg aatcatagtt cctggaggat ttggtaaccg cggaattgaa
     2221 ggaatgatag atgcaattga atatgcacga atcaataact taccattttt tggaatttgt
     2281 ttagggatgc agctggcaac gattgaatac gcccgtaatg tttgtggctt gaaagatgct
     2341 aattcattag aatttgatga actaacaaaa aatcctatta ttaatttaat gtctgatcaa
     2401 tcattaactg atatgggtgg aactcaacgc ttaggtgatt ataattgtga actggctgcc
     2461 ggtacacacg ctagagaatt atatggggtc gatatgattc aagaacgtca tcgtcatcgc
     2521 tatgaattta ataatgagta taaagacgct ttagttgaac atggacttac gattgcaggc
     2581 attaatcctg aacgtaattt ggtagaaata gtggaaatta aggaacatcc atattttgtg
     2641 gcttgccaat ttcatccaga atttacaagt cgaccaaatc gttctcaacc gctatttcag
     2701 ggtttgatta cagccgctca taagcgaaaa tatcatgggt aggtattgca cctatccttt
     2761 ttatatagta ccgtaatatg atacttagtg aataaatagt aactttagtc ctaaatcaag
     2821 atacaaactc ccctgattag tcattatcat tatattcgct tacaactata ataattgata
     2881 taggaggaag taaaatgaga aaaataaaaa aactatttgc aatgatgatg acattagtaa
     2941 tgaccctagg cattattaca acaaatatta gtgcaatcga ggaattaaaa gttacttcaa
     3001 ttgttgctaa tacatatgtt ggggatggac aaagagaagt gagttcattt gaaatcactg
     3061 tcaatgatat taatttagta aatgatttaa aggctgaaga ttttgatatt actaataacg
     3121 ttagctcagt accctacgac gttgcgacaa atgctttagc agatgattat ttagatgatg
     3181 gaattagttt agaaattaaa ggaaatacaa ttattcttaa tgtaaaagcc tttgattata
     3241 ctggacgata caatcctgat tttagtaaaa atccttggca ggttacctgc aacaaatatg
     3301 gtgtgttaag tttcaataaa gataatgtga ctacattgaa aacaaaaact ttggatgatg
     3361 caatcagagg aacgtttaca tatgcgggat taactcgtga gtatgcttta tatttgccta
     3421 aaaatagtga tggaactaac atgaaaaatg ttccattagt agtatggaat catggtggtg
     3481 gagaatatgc agggcaatta gaaaatacac ttgttgctaa tagaggttta actgcatggg
     3541 tagaggcagg atataataca gcggtattac aaattcaagt ttctaatcca aactatagct
     3601 acgggacagc ttttgatgaa gataagaaat ctttgatcga tcaaaataac gctttacaag
     3661 cagcgttaat aaaaaaatta attgcagaag gaacagttga tcaaaatcgt gtttatgtta
     3721 caggagcatc aagtggtggt ggcgcaacga tgcgctttat tatgcaatac ccagaacttt
     3781 ttgcgggtgc gatagcttgt tgttcaatgg atccgattgt gtgggtacat tataattatc
     3841 aagattctta tgaacaaatt gttgaaaatt ttgaaactgc tttccaagga caagtataca
     3901 cttgggatga aacaaaagaa atgatggtaa gcaaacaagt tgatactgct tctttagtag
     3961 agttaccaat ttattttaca catgctcaaa atgatactac atgcaatgtt aatagttcaa
     4021 aagctatgta tgaagcttta aataatctag gtgctaaaaa taatcactta acaatctggt
     4081 caaatcaaga aatgactgac gaaggaattg ctgaaggttt attacattgg tcgtgggtaa
     4141 aagtacttaa ccataatgaa gaaggttcac caatgaattg gttatttaaa cagacaaatg
     4201 cttcagaaaa tattccagta gaaaaagaag agtcgaataa ttctaaaact gaaacaactg
     4261 cttctgagga agcaaagaaa gcagttaaaa caggtgatga tagtacagtt gaattgatgg
     4321 ctatgatgac tggattatca ttgttagcgg gaacagtagt attaagtaaa aaatattatt
     4381 aataataaaa atcctctaac tgaaatgaac ctcattattt gaacgttcaa ataatggaag
     4441 attatttcaa atgaggattt tttatttaaa attcgagtaa tttcaccaaa aaattaatga
     4501 aatatagtat actaataccc agaggtgaaa gcgctatgat aaaaaatata actttattag
     4561 atagtatttt ggatgttgct aactgtgtcg atattaatga taataatacc tatattgctc
     4621 ggaaagtgat agagaattgt gtttggttaa aggatatgag tttagaggat ttcttagaaa
     4681 caactaatat taatgggagt cagtttaaac gattttataa attgtatgac tgcaacaatt
     4741 tttcaatctt gaaagaacgg attggcttgt ggtgtgatat tcgtaaagag caagtagtaa
     4801 aacagtgtca gtggcaaaaa caagaaccgc ttgcaagagt tatttttaac ttaactaatt
     4861 ataatgattt taatgatttt tttaatgttg agttaattga tcggatttgc aagcaaatct
     4921 atcaaagtaa gcgtattatc atgtatgggg cgatgggatt gttgaattta acacatgatt
     4981 ttcaaataga tatgaaactg tttggaaagg attttattcg tagttcgatg tatgaagata
     5041 aggctctagt tcctcaaaaa gatgattttg tatggctttt ttcaatgatg ggaagaacga
     5101 tgaatat
//
LOCUS       DAOMTA010000104         5106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_361_length_5106_cov_3.570580, whole genome shotgun sequence.
ACCESSION   DAOMTA010000104 DAOMTA010000000
VERSION     DAOMTA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_5106_cov_3.570580"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLK68_04130"
     CDS             complement(<1..443)
                     /locus_tag="PLK68_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRM89630.1"
                     /translation="MPVNPYNLDMITKICNHLNTACAIITYRHNFHFEYANDLYYQLF
                     KYKRNDGFNYSLPYSNDYQKVKNTITSIIQKKEEFLEIETQSFNKDNDLIWTRSRLSF
                     IYNNQSVYIICLVENITENKKVLKNLEISHQKYLHEGQFKAAIAS"
     gene            complement(519..1313)
                     /gene="thiD"
                     /locus_tag="PLK68_04135"
     CDS             complement(519..1313)
                     /gene="thiD"
                     /locus_tag="PLK68_04135"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRM89631.1"
                     /translation="MKKVLTIAGTDPTGGAGVQADLKTMTAHKVYGMSIITALVAQNT
                     LGVRDIMEVKPDFLAEQFDCVFEDIYPDAIKIGMVSSPVLIEMIVNKLTSQKDCPIVV
                     DPVMVSTSGSRLLADNALRLLKEKLIPLATIITPNIPEAQVLTNLKINTKDDMITAAK
                     MISEWYHGYILIKGGHFEERADDLLYYRGNITWLTGEKINNPNTHGTGCTLSSAIASN
                     LALEYSIEESVTRAKVYITGALKANLNLGHGSGPLDHCWNINQTLK"
     gene            complement(1316..1954)
                     /locus_tag="PLK68_04140"
     CDS             complement(1316..1954)
                     /locus_tag="PLK68_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM89632.1"
                     /translation="MIKGYIIDMDGTLLDSMHIWNELGSRFLELKGITPEANLKDILA
                     PLSINQAIKYIAETYQLKEPLDVLINEVNSLLNHIYLSEIPLKPGALEFITNCFNHHK
                     KLCLLTANNYQATINILDKYNLTSKFDEIITCDHTTLDKRSGEAYNYAISALHLHKDE
                     CIVIEDALHAIIAAKKQGFTVWAVADQSNQDDWDEICKISDLNLKNLSEMER"
     gene            complement(1947..2594)
                     /gene="thiE"
                     /locus_tag="PLK68_04145"
     CDS             complement(1947..2594)
                     /gene="thiE"
                     /locus_tag="PLK68_04145"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM89633.1"
                     /translation="MLKLNFTRNMLELYLVSDRSWLNDRPLEEDIEQAILGGVTMVQL
                     REKNLTDEEFTIQAKKVKTICSKYHIPFIINDNVAVALAVDSDGIHIGQDDQPVKRVR
                     KIIGPHKIIGVSAHNLKEALAAKEDGADYLGVGAMFNTSTKDDATAVSFTQLHEITTK
                     IGLPVVAIGGINQDNCLLLKGTKIDGIAVVSAIMSAPDIKEAAAKLKAHARGIYD"
     gene            complement(2572..3396)
                     /gene="thiM"
                     /locus_tag="PLK68_04150"
     CDS             complement(2572..3396)
                     /gene="thiM"
                     /locus_tag="PLK68_04150"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRM89634.1"
                     /translation="MFEKVLEEIKLRNPIVHCITNYVTVNDCANAILAVNGSPIMADD
                     IHEVEEITTICNALVINIGTLNERTVASMIKAGKMANHLNHPVVLDPVGAGASKLRTN
                     TAKKLLEEINFSVIRGNISEIKALAMNMTSTQGVDANINDIVTAENLNEVISFAKKFS
                     QETGAVIAITGAIDIVANQEKTYVITNGCAMMSRITGTGCMLSAILGATTAIGQNDLL
                     ETTAYTIAMMGYCGELADQRVRNDNSGTSSFRMHLIDALSTINYYQLKAGVKIELH"
     regulatory      complement(3457..3562)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3702..>5106
                     /locus_tag="PLK68_04155"
     CDS             3702..>5106
                     /locus_tag="PLK68_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM89635.1"
                     /translation="MKNNYDVVVVGAGPAGIMACYELYLKQPELNVLLIDKGQDVMKR
                     HCPIKEKKIKSCPIIRNNEPGCLPACSITAGFGGAGAYSDGKFNITSEFGGWLTDYLS
                     TNEVEDVINYVDNLYLKHGATREITDPTTDKVKEIEHRGYAVGLKLLRAKVRHLGTEE
                     NLRIMTEMSTELKEHIDMAFRTAVKEVLVEDGHAVGVVLENDEVIKAKKIVLAPGRDG
                     SAWLTKVLKQHGLELYNNQVDIGVRVETSNIVMEEINSNLYEGKFVYNTSVGTKVRTF
                     CSNPSGHVVIENHSGTMLANGHAYHDPKLGSKNTNFALLVSHTFSEPFNEPNEFAHEV
                     SRLANKLSNGSVMVQRYGDIKKGRRTTYKRLKEGYTEPTLAEAVPGDLGLVLPYNTMK
                     SIIEMIEALDNVTPGIANEHTLLYGVEAKFYSARPKVREGFECEIDDLYVAGDGAGLT
                     RGLAQAGANGIIVARHII"
BASE COUNT         1596 a          904 c          984 g         1622 t
ORIGIN      
        1 tctgatgcta ttgctgcttt aaactgtccc tcatgaagat atttttgatg actgatttct
       61 agatttttta aaactttttt gttttcagta atattttcga ctaaacaaat tatatatact
      121 gactgattat tgtatataaa ggatagtctg gaacgagtcc aaatcaagtc attatcttta
      181 ttaaatgatt gagtctcaat ttcgagaaat tcttctttct tttgaatgat actagtaatt
      241 gtatttttta ctttttgata atcattagaa tagggaagag aatagttgaa gccgtcatta
      301 cgcttgtatt taaaaagttg ataataaaga tcattagcat attcaaaatg aaaattatga
      361 cgatatgtaa tgattgcaca agcagtattt aaatggttac agatcttagt gatcatatca
      421 agattatatg gattaactgg cattctattt tcctccttat taaattagaa aaaatagctc
      481 tacctcatta tactactaaa gcaattatat cggttgaatt attttaatgt ttgattaata
      541 ttccagcaat gatctaatgg tccactgcca tgacctaggt taagattagc ttttaaagcc
      601 ccggtaatat agactttagc tctagtaacg ctttcttcaa ttgaatattc taaagctaga
      661 ttggaagcaa ttgctgaaga aagggtacag ccagtgccat gagtattagg attattaatt
      721 ttttctcctg tcaaccaagt aatatttccc cggtaataaa gaaggtcatc agcccgttct
      781 tcaaaatggc cccctttgat caaaatgtag ccgtggtacc attcactaat catctttgcg
      841 gctgtaatca tgtcatcttt agtattaatt tttaaatttg ttaaaacctg tgcttcaggg
      901 atattagggg taataatagt agctaatgga ataagctttt ctttcaagag tcttagagca
      961 ttgtcagcta ataaacgaga tccgctagta gaaaccataa caggatcaac aacgatagga
     1021 caatcttttt gtgatgttaa tttatttacg atcatttcaa ttaatactgg actggaaacc
     1081 attccaattt taattgcatc aggataaata tcttcaaaga cacagtcaaa ctgttcggct
     1141 aaaaaatcag gttttacttc cataatatct ctaacaccta atgtgttttg ggccactaag
     1201 gcagtaataa tactcatccc gtagacttta tgagctgtca tggtttttag atccgcttga
     1261 actcctgctc cacctgttgg atcagttcct gcgatagtta gaactttttt cataatcatc
     1321 tctccatttc tgataaattt tttaaattta aatcacttat tttgcaaatt tcatcccaat
     1381 catcttgatt actttgatct gcaacggccc atactgtaaa accttgcttt tttgcagcaa
     1441 ttattgcatg aagggcatct tcaataacaa tgcattcatc tttatggagg tgtaatgcag
     1501 agattgcata attgtaggcc tcgccactgc gtttatctaa agtagtatga tcacatgtaa
     1561 taatttcatc aaattttgat gttagattat atttatcaag aatgttaatt gttgcttgat
     1621 aattatttgc agttaataag cacagttttt tatgatggtt aaaacagtta gtaataaatt
     1681 ccaaagcccc tggttttaag gggatttcgc ttaaataaat atgattcaat aaactattaa
     1741 cttcattgat taaaacatca agaggctctt ttaattgata tgtctcagcg atgtatttaa
     1801 ttgcttggtt aattgatagt ggtgccaaga tatctttaag atttgcctca ggggtaattc
     1861 cttttaattc gagaaaacga ctgcctaatt cattccaaat gtgcatcgaa tcaagcaaag
     1921 ttccatccat gtcaatgata taacctttaa tcatagattc ctcgggcatg tgcttttaat
     1981 ttagcggctg cttcttttat atcaggagcg ctcataatgg ctgaaacaac agcaatacca
     2041 tcaatttttg ttccttttaa gagtaggcaa ttgtcttggt tgataccacc gattgctaca
     2101 acaggtaagc caatttttgt agttatttca tgtaattggg taaagctcac tgctgtagca
     2161 tcatctttag ttgaagtatt gaacatggct cccactccta aataatcagc accatcttcc
     2221 ttggcggcaa gagcttcttt taagttgtga gcagaaaccc caataatttt atgaggaccg
     2281 attatttttc gaactctttt aacaggctga tcatcttgac cgatatgaat tccatcactg
     2341 tctacagcca atgctacggc tacattatcg ttgatgataa agggaatatg gtatttagaa
     2401 caaattgttt tgactttttt tgcttgaata gtaaattctt catcagttaa atttttttcg
     2461 cgtaattgaa ccattgttac tccacctaaa atagcttgtt caatatcttc ttcaagtgga
     2521 cgatcgttca accagctgcg gtcgctgact aaatatagtt ctagcatgtt tctagtgaag
     2581 ttcaatttta acaccggctt tcaattgata ataattaatt gtactcaagg catcgattaa
     2641 atgcattcta aaactgcttg ttccactatt gtcattacga actctttgat cagctaactc
     2701 accacagtat cccatcattg caatcgtata agcagtagtt tctaaaagat cattttgacc
     2761 aatagcggtt gttgctccaa gaattgccga taacatacaa cctgttcctg tgattcgtga
     2821 catcattgca caaccgttag tgattacata ggttttttct tgattggcaa caatatcaat
     2881 tgctccagtg attgcgatta cggcaccggt ttcttgacta aatttcttag caaaactaat
     2941 aacttcatta agattttcag ctgttacaat atcattgata ttggcatcga ctccttgagt
     3001 tgaagtcata ttcattgcta gggcttttat ttcggaaata tttcctcgaa taacactaaa
     3061 attaatttct tcaagtagtt tttttgcagt attagtccga agtttgctag ctccagcacc
     3121 aacaggatcg agaacaacag ggtgatttaa atggttagcc atttttcctg ctttgatcat
     3181 tgaagcaact gtcctttcat tcagcgtacc aatattgatt actaaagcat tacagattgt
     3241 tgtaatttcc tcaacttcat gaatgtcatc agccataatg gggctgccat taacagctaa
     3301 gatggcatta gcacaatcat tgacagtaac atagttagta atgcaatgca caattggatt
     3361 gcgtaattta atttcttcta aaactttttc aaacatattc ttcctcactt tcttaaagca
     3421 ctaaaaagag aatatgccaa gtgccatatt ccctacttat tgttagtgag tatgtcccta
     3481 cgttggcatt atccaaatca ggttagcggt cgaagttcac aacttcctct cagcctgttt
     3541 acaagctccc gtgctttaac tatatcatag cattttcaaa ctatttcagc aaactaagta
     3601 tcaaaatttt tttgcgttga taataaaaga agattgaaat gcataattat atcgagtata
     3661 attagtaggg ttagcgttaa aacccagaac ggagcgataa aatgaaaaat aattatgatg
     3721 ttgtggttgt tggagcggga ccagcaggaa tcatggcctg ctatgaactg tatttgaaac
     3781 agccggaatt gaacgtattg cttatagata aaggtcagga tgttatgaaa cgtcactgtc
     3841 caatcaagga aaagaagatc aagtcctgtc caatcatcag gaacaatgaa ccgggctgtc
     3901 ttcctgcctg ttcgattaca gccggatttg gcggtgccgg tgcctattct gacggtaaat
     3961 tcaatattac cagtgaattt ggcggctggc tgactgacta tctttcaacc aatgaggttg
     4021 aagatgtaat caactatgtt gacaatcttt atttaaaaca cggagcaacc agagaaataa
     4081 cggatcctac gactgacaag gtcaaagaaa ttgaacatcg aggctatgca gtaggattaa
     4141 aactgctgcg ggccaaggta agacatttag gaacagaaga gaatctgcgg atcatgacag
     4201 aaatgtcaac ggaactgaaa gaacatattg atatggcatt tagaacagct gtaaaggaag
     4261 tactggttga agacggacat gctgtcgggg tagttctgga aaatgatgag gtcatcaaag
     4321 cgaagaagat cgtactggca ccgggacgag acggttctgc ctggctgaca aaagtactga
     4381 aacagcatgg actggagctt tataataatc aggtcgatat aggagtgcgg gtagagacca
     4441 gcaatatcgt aatggaagag atcaacagca atctgtatga aggtaaattt gtctataata
     4501 caagtgttgg aacaaaagta agaacattct gttcaaatcc gtcagggcat gtagtaatag
     4561 agaatcacag cgggacgatg ctggcgaacg gacatgccta tcatgatccg aagctgggga
     4621 gcaagaatac caactttgcg ctgctggtat cccatacctt cagcgagccg ttcaatgaac
     4681 cgaatgaatt tgcacatgag gtgagcagac tggcgaacaa gctgtcaaac ggatcagtca
     4741 tggtgcaaag atatggagat atcaagaaag ggagaagaac gacttacaag cgtttgaaag
     4801 aagggtatac agagccgacc ttagcagagg cggtaccggg agatctgggg ctggtattac
     4861 cgtacaacac gatgaaatca attatcgaga tgattgaggc actggataat gtaacaccgg
     4921 ggatagcgaa tgagcatacg ttgctgtatg gagtggaagc gaaattctat tcagcgcggc
     4981 caaaggtaag agaagggttt gaatgtgaaa tagatgatct gtatgtagca ggtgatggag
     5041 ccggattaac aagagggctg gctcaggcag gagcaaatgg aatcatcgta gcacgtcata
     5101 ttattg
//
LOCUS       DAOMTA010000105         5105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_362_length_5105_cov_2.737822, whole genome shotgun sequence.
ACCESSION   DAOMTA010000105 DAOMTA010000000
VERSION     DAOMTA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_5105_cov_2.737822"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1516
                     /locus_tag="PLK68_04160"
     CDS             107..1516
                     /locus_tag="PLK68_04160"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM89636.1"
                     /translation="MKLKENFLWGGATAANQYEGGYNEGGRGLSINDVEKGAKHGVPR
                     EIHEYIHENTYYPSHVATDFYHHYQEDIKLFAQMGFKCFRMSISWSRIFPRGDEEQPN
                     EEGLAFYDKVFDELLKYGIEPVVTLSHYETPLTLVNEYGSWRSRKLVDFFEHYCKTVF
                     SRYKDKVKYWMTFNEINGCLEVARPWHQAGIVYRDDEDHYQTILQASHHMFVASAKAV
                     IAGHEINPNFKIGCMLIYPTTYAATCNPEDQIMMRNKMLNTFYYGDVHVRGRYTNTCT
                     SLLHKHGVEIKMEPGDEELLAKGKVDYIGFSYYFSAVEGNDAEEIEGNVVKGGRNPYL
                     KMTDWGWQIDPLGLRTALNELYDRYQLPLFIVENGMGAVDTVEVDGSINDAYRIDYIK
                     EHIKAFKDAVEIDEVDLMGYTPWGCIDLVAASTGEMRKRYGFIYVDKDDEGNGTLARK
                     PKKSFYWYQNVIKTNGEEL"
     gene            1580..2083
                     /locus_tag="PLK68_04165"
     CDS             1580..2083
                     /locus_tag="PLK68_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HRM89637.1"
                     /translation="MENYCRKMAWLLYNRGLVDKENVEDLRFLLELILTQVITIISVY
                     IIGLLFMDALSILIICACFVAGRKYLDGYHADTFRNCYLLTMLNFIFCIFMSRIVAYV
                     EIFYFIGIGISLYLLFKYRYKKIIIFNLFYILIIVLFNTGIVYQINMVNYFVIVLMRV
                     IRGEELK"
     gene            2080..>2529
                     /locus_tag="PLK68_04170"
     CDS             2080..>2529
                     /locus_tag="PLK68_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM89638.1"
                     /translation="MISIGIIDDEQVYLDKIKDILITNFDDIRVYSYNSASKIDNDLD
                     FILLDIDMPDIDGIIFSKQHRNYRIVFVTNYDTRIKEAFGPNVYGYVSKGNLEEELVE
                     KVKEVIEVIKCDYYVTFKVNGIDINIRIDDIIYCQYLGNHIVSIVYHD"
     assembly_gap    2530..2539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2540..2750
                     /locus_tag="PLK68_04175"
     CDS             <2540..2750
                     /locus_tag="PLK68_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM89639.1"
                     /translation="ININNSSLKKVKEVLNEYFIEISQDIIINKHRIINFEDRYVYLD
                     GINSKFEVSVRKRKLVRKSFYETFR"
     gene            2747..3982
                     /locus_tag="PLK68_04180"
     CDS             2747..3982
                     /locus_tag="PLK68_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRM89640.1"
                     /translation="MIEVISEYFYCLIYVIALTFTASYLLEEKVKFKRVVIMAPLYAI
                     VNYLVAGATSNNFWEYMIVNSIIIVIDFCYICILFKKTNVIFYVSLFQLFYIFTVNSL
                     IHVINIIIGFNREVTLSFSIQRLIMVFLINILTVIFIVVLDKLKIIPTQKVINRQVKL
                     FIMLDILVYYAMVIIYNIGVVRLVNLITVLVLILLFLWIMFLKILSMYVETTIRNEEL
                     IMEDISNKYISKYLDFYNQESDNLRKLKHDLKNHQLVLESLDKKNQYTQYIDEVFKGI
                     GQVTYIESGNIYIDACLYAKQQEYPEIIFDFDISVAGLVFNEKDLTSLIFNLIDNACN
                     EALKHNKLVSVMIRYTNNLLIIRIKNTCPIKPNFSTDKGEGHGYGLKIIKNIVNKYHG
                     DLFIDYHDDQVIFNIKINT"
BASE COUNT         1859 a          497 c          961 g         1778 t
ORIGIN      
        1 tataaatcgc taatcaataa tattagcgat tttcttttta aattatgaaa aaataaatat
       61 tgtcaaataa atgatgataa aatcatggta atttggaggt actaaaatga aattaaaaga
      121 aaatttttta tggggtggcg ctacagctgc gaatcaatat gagggtggct ataatgaagg
      181 tggtcgtgga ttaagtatta atgatgttga aaagggtgct aaacatggag tacctcgtga
      241 aattcatgag tatattcatg aaaatacata ttatccatca catgtagcta cagattttta
      301 tcatcattat caagaagata ttaaactatt tgcccaaatg ggtttcaaat gttttagaat
      361 gtcgatttca tggtcaagaa ttttcccacg tggtgatgaa gaacaaccta atgaagaagg
      421 tttggctttt tatgataaag tatttgatga gttattgaaa tatggaattg agccagttgt
      481 aaccctatca cattatgaaa caccattgac tttagttaat gaatatggtt catggagaag
      541 tcgtaaatta gttgatttct ttgagcatta ttgtaagacc gtattttcta gatataagga
      601 taaggttaaa tactggatga cttttaatga aattaatggc tgtttagagg tggctcgtcc
      661 ttggcatcaa gcgggaatcg tttatcgtga tgatgaagat cattatcaaa caattctcca
      721 agctagtcat catatgtttg tagcaagcgc taaagcagtg attgcagggc atgaaattaa
      781 tccaaatttt aagattggct gtatgttaat ttatccaaca acatatgcag caacttgtaa
      841 cccggaagat cagattatga tgagaaataa gatgctaaat actttctatt atggtgatgt
      901 tcatgttcga ggacgttata caaatacatg tacttcatta ttgcataaac atggtgttga
      961 aataaaaatg gagcctggag acgaagaact tttagcgaaa ggtaaagttg attatattgg
     1021 ttttagttat tatttttcag ctgttgaggg taatgatgct gaagaaattg aaggaaatgt
     1081 tgttaaaggt ggacgtaatc cgtatttaaa gatgacagat tggggttggc aaattgatcc
     1141 attaggatta agaacagctt taaatgagtt atatgatcgt tatcaattac ccttgtttat
     1201 tgtagaaaat ggaatgggag cagtagatac agttgaagtc gatggttcaa ttaatgatgc
     1261 ttaccgtatt gattatatta aagaacatat caaagcattt aaagatgcag ttgagattga
     1321 tgaagttgat ttaatgggat ataccccttg gggatgtatt gacttagtgg cagcaagtac
     1381 cggtgagatg agaaaacgtt atggttttat ctatgttgat aaggatgatg agggtaacgg
     1441 aacccttgca cgtaaaccaa agaaatcatt ctattggtat caaaatgtta taaaaacaaa
     1501 cggtgaggag ctgtaatgac ttaggtcgtt acagtttttt gtagaatgtg ttaaaatatt
     1561 ctacaaaggg gagagtttta tggaaaatta ttgtcgcaaa atggcctggc tcttatataa
     1621 tagagggcta gtcgataaag aaaatgttga agatctgcgc tttttgctcg aacttatttt
     1681 gacacaggtt ataacgatca tatcagtcta tataattggt ctattattta tggatgcact
     1741 gagtatcttg attatttgtg cctgttttgt agccgggcga aaatatctag atggttatca
     1801 tgccgatact tttagaaatt gttatttact gacgatgtta aactttattt tttgtatttt
     1861 tatgagtagg atagtagcat atgttgaaat attttatttt ataggaatag gaatatcatt
     1921 atatttatta tttaaatata gatataaaaa aataattatt tttaatttat tctatatatt
     1981 gataatagta ttatttaata caggtatagt ttatcaaatc aatatggtta attattttgt
     2041 aatagtattg atgagagtga taagagggga ggaattaaaa tgatctctat tgggataatc
     2101 gatgatgaac aagtatattt agataaaatt aaagatattt taatcacaaa ttttgatgac
     2161 attagagtct attcgtataa ttctgcgagt aaaatagata atgatctaga ttttatatta
     2221 cttgatattg atatgcccga tatagatgga attatttttt caaaacaaca tcgtaattac
     2281 agaattgtat ttgtgactaa ttatgatact cggatcaagg aagcttttgg acctaatgta
     2341 tatggatatg tatcaaaagg aaatcttgaa gaagaattag tagagaaagt taaagaagtg
     2401 attgaggtaa tcaaatgtga ttattatgtt acgtttaagg taaatggaat agatataaat
     2461 attaggattg acgatataat ttactgtcag tatttaggta atcatattgt ttcaatagtc
     2521 tatcatgatn nnnnnnnnna attaatatta ataatagttc tttgaagaaa gtaaaagagg
     2581 ttttgaatga atattttata gaaataagcc aggatataat aataaataaa catcgaatta
     2641 ttaattttga agatagatat gtgtatctag atggaatcaa cagtaaattt gaagtaagtg
     2701 taagaaaaag aaagttagtg agaaaaagtt tttacgagac atttagatga tagaagttat
     2761 tagtgaatat ttttattgtt taatttatgt tatcgcactt acatttactg cgagttatct
     2821 attagaggaa aaggttaaat ttaaaagagt agttataatg gcaccgctat atgcaattgt
     2881 aaattatctt gtagcagggg ctactagtaa taatttttgg gaatacatga ttgtgaattc
     2941 tattatcata gtcatagatt tttgctatat ttgtatttta tttaaaaaaa caaatgtaat
     3001 attctatgtt tctttattcc agttatttta tatatttact gttaattctc taatccatgt
     3061 tattaatatt attattggat ttaaccgtga ggttacatta tcatttagta ttcaaaggct
     3121 tatcatggtt tttttaataa atatattaac agttattttt attgttgttt tggataaatt
     3181 aaagattatt cccactcaga aagtaataaa tcgtcaagta aaattattta tcatgctcga
     3241 tatattagtt tattatgcga tggtaattat atataatatt ggtgtagtaa gattggttaa
     3301 tttaattacg gtattagtat tgattctatt atttttgtgg ataatgtttt taaaaatatt
     3361 gtctatgtat gttgaaacaa caatcagaaa tgaagaattg ataatggagg atatatctaa
     3421 taaatatata agtaagtatc ttgattttta taatcaggaa agtgataatt taagaaaatt
     3481 aaagcatgac ctaaagaatc atcaacttgt attagaaagt ctagataaaa agaatcaata
     3541 tactcaatat attgatgaag tatttaaggg aattggtcaa gtaacatata ttgaaagtgg
     3601 taatatttat attgatgctt gtttatatgc taaacaacaa gaatatcctg agataatttt
     3661 tgattttgat ataagtgttg cgggtttggt atttaatgaa aaagatttaa catcattaat
     3721 atttaattta attgacaatg catgtaacga agcattaaaa cacaataagt tagtatcagt
     3781 gatgattaga tatactaata atttattaat cattagaatt aagaacactt gcccgattaa
     3841 gcctaatttt agtacggata aaggtgaagg gcatgggtat ggattaaaaa taattaaaaa
     3901 tattgttaat aaatatcatg gtgatttgtt tatcgattat catgatgatc aagtaatatt
     3961 taatattaaa ataaacactt aaggaggtat ttttaacata tgaggaggtg taaaaaaaca
     4021 ttatctcgat ttatgttata ttttagaagt gaggaggata tgtagatgaa aaatatgatt
     4081 aaaaaagtta ttaaaaaaag aatagaaaaa gtaaaactta atggttataa ttgttcacct
     4141 tgtgattatt atagaccaaa aaaaccaagt aaataatagt acaaactttt gggagagggt
     4201 aaatgtacta aatagtaata tttattaatg gcatagccaa agcaaaaatt tatataaata
     4261 agattcaata taatcaattt ctataaagat agaatcgtcg tacatataca ttaaatggga
     4321 taaagcgtat ataataaaaa gtgattttta ttattactga gggttgttag catggctttt
     4381 ttattataga gacgagattt tgaacctttg tttagtaaat tatgttataa aatagctgga
     4441 ttaataatat gttcgattaa aaagagatat taattattta atatctcttt ttagcgtgtg
     4501 ccgggcatgg ctggcatgaa ttcagttaga gataaaccaa ccacaggtag ttaccgtcaa
     4561 gcgtagaaaa ccacaacctg ttgacagtaa tgacatgggt gaaggaagta atgtgatgta
     4621 gcgattcttt tgaacatgtg aacaggaact taatgaggct aaatggtgga taaggttgcg
     4681 ctacaaacca aagtccaaat ggtatatgta agactaagac agtaaatcaa gacattgtga
     4741 aagaattagg attcatgctc tacttcgaga ggctttatgg acgaggaagt ctagtaaact
     4801 ttaaaatcaa tgatagtatc ctcggtagaa aaaggtttat cgtaagaagt tagatgaagt
     4861 catagtaggt agagatactg gaggactaaa acgtttatag agtgcaaatt tagtaatgaa
     4921 gacatcttta aatgtgctaa tatagaatta gggcggtata gtaggatgaa tgtcataaac
     4981 tttattatat taccgaaaat attaggtatt aagaaaggga aagacctgga ttggtacatc
     5041 gcctaaatta ctatcttaag agtttgtaat ttgatataaa tgtagagccg tatactagac
     5101 ctgca
//
LOCUS       DAOMTA010000106         5097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_364_length_5097_cov_2.475010, whole genome shotgun sequence.
ACCESSION   DAOMTA010000106 DAOMTA010000000
VERSION     DAOMTA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_5097_cov_2.475010"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="miaA"
                     /locus_tag="PLK68_04185"
     CDS             <1..847
                     /gene="miaA"
                     /locus_tag="PLK68_04185"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRM89641.1"
                     /translation="AKHFNGEVISGDSMQIYKTMDIGTAKVTDDEMEGIVHHLIDVKE
                     PTESYSVKDFQDEVRLKIKEIISRGKLPIIVGGTGLYIKAALYDYEFSESQTNHQEYV
                     EKYQDYSNEELYNYLIEIDKASAKELHPNNRQRVLRAIAIYESTGVKKSETLAKQNHE
                     LIYNAKFIGLTLERESLYQRIDQRVDLMMEQGLLQEIDGLMKKNYTREMQSMKAIGYK
                     EWFAYYQGTQTLDETLELIKKNSRNYAKRQYTWFNNQVPVIWFNVNLNNFNETINAVI
                     SELEG"
     gene            959..>1629
                     /locus_tag="PLK68_04190"
     CDS             959..>1629
                     /locus_tag="PLK68_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM89642.1"
                     /translation="MMFNDNLNKYRKQKGLSQEELAFRLGVSRQSVSKWESGQSTPEL
                     ERIIEIADLFGISLDELIGHESNDYVTVDREELRSVVRHLFTYEYKSKFKIGNVPLIH
                     INLGRGFRIAKGIIAIGNIAVGLFSFGALALGVFSLGGLAIGLLTLAGIAIGGIGLGG
                     LSIGYFAVGGMAIGIYAIGGMAIATKLAIGGMAHGYVAIGSSANGVHTLVSTNCSLEM
                     ISNFI"
     assembly_gap    1630..1639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1819..3009)
                     /gene="thiC"
                     /locus_tag="PLK68_04195"
     CDS             complement(1819..3009)
                     /gene="thiC"
                     /locus_tag="PLK68_04195"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRM89643.1"
                     /translation="MNYTTQMNAAKKGIITPEMKIVAAKEKMNIEELKTLIAQGKVVI
                     PANKYHTCINVNGIGSMLKTKINVNLGTSRDWKDLDMELKKANDAVNMGVESIMDLSS
                     YGDTQKFRRKLTKECPAIIGTVPIYDAVVYYHKPLKEITARQWIDIVEMHARDGVDFM
                     TIHVGINKNTAQRFKENKRLTNIVSRGGSIIFAWMEMTGQENPFYEYYDEILDICQKY
                     DVTMSLGDACRPGSIEDAGDISQIEELVTLGELTKRAWQKDVQVIVEGPGHMALNQIE
                     ANIKIQQTICQGAPFYVLGPLVTDIAPGYDHITAAIGGALAAANGAAFLCYVTPAEHL
                     RLPDLNDVKEGIIASKIAAHAADIAKGIKGAKDLDYQMSCARKNLDWEKMFELAIDPK
                     KART"
     regulatory      complement(3077..3184)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3222..4487)
                     /locus_tag="PLK68_04200"
     CDS             complement(3222..4487)
                     /locus_tag="PLK68_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRM89644.1"
                     /translation="MSSTLANRMRPQNLKDIIGQQHLVGPNAILTKFVQKSHPFSIIL
                     YGSPGCGKTTIAMALANDLDIPYRIFNASTGNKKEMDAIIEEAKLSGSLFVIIDEVHR
                     LNKARQDDLLSHMESGLLIVAGCTTANPFHSINPAIRSRCHILEVRPLTVDEIVAGLN
                     KAMTSPNGLNSKYTIEKEALHSIAKLCSGDIRYGYNCLEICTIVCENNHITQADLKTA
                     AIKTNVVYDKDEDNYYDTLSGLQKSIRGSDPNGAMYYFAKLIESKDIESLERRLITTA
                     YEDIGLANPNACMRTVIAMQAAKTIGFPEARIPIASAIIDLCLSPKSKSSENAIDAAL
                     TSLNERSLKTPSYLRLTPVGLEDDEKYDYSRPELWEYIQYLPKELGNTQFYVPWMTSN
                     YEKALAENYRRILKHGRTSDIKKLNQQNK"
     gene            4564..5016
                     /gene="dtd"
                     /locus_tag="PLK68_04205"
     CDS             4564..5016
                     /gene="dtd"
                     /locus_tag="PLK68_04205"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRM89645.1"
                     /translation="MRLVVQKVSQSSVKIEGEIVGAIDKGYMVLVGITNGDDELLVEK
                     MVDKLVNLRIFEDENDKLNLSLLDVGGSVLSISQFTLYANCKKGRRPSFIDAAKPDIS
                     SPLYDFFNKKLEEKGINVERGVFGAMMEVSLINDGPVTIILDSNELFY"
BASE COUNT         1655 a          764 c          938 g         1730 t
ORIGIN      
        1 agctaagcat tttaatggcg aagtgattag cggtgattcg atgcaaatat ataaaacaat
       61 ggatattgga acggctaaag taacagatga tgaaatggaa ggtattgttc atcatttgat
      121 tgatgttaag gaaccaactg aaagctatag tgtaaaagat tttcaagatg aagttcgttt
      181 aaaaataaaa gaaataatat cacgtggtaa attgccgatt attgtaggtg gtacaggatt
      241 atatattaaa gcagcacttt atgattatga atttagtgaa agtcaaacca atcatcaaga
      301 atatgttgag aagtatcaag attattcaaa tgaagagtta tacaattatt taattgagat
      361 tgataaagca agtgctaaag agcttcatcc taataatcgt caacgggtct taagagcaat
      421 tgcaatttat gaaagtacag gggtaaaaaa aagtgaaaca cttgccaaac aaaatcatga
      481 attaatttat aatgctaaat ttattgggct gacattagaa cgtgaaagtt tatatcagcg
      541 gatcgatcaa cgtgttgatt tgatgatgga gcagggattg cttcaagaaa tagatggctt
      601 aatgaaaaag aattataccc gtgaaatgca aagcatgaaa gcaatcgggt acaaggaatg
      661 gtttgcttat tatcagggaa ctcaaacact tgatgaaact ttggagttga taaaaaagaa
      721 ttctcgtaat tatgcaaagc gtcaatatac ttggtttaat aatcaagttc cggttatttg
      781 gtttaatgtg aatctaaata attttaatga gacgattaat gcagttatca gtgaattaga
      841 agggtaatta tatcctttta ttgtttatgc aactgttagt tgacagattg cctagccgta
      901 gtttattgtg atttggaata aaaaaactat aatcatggtt gtcaggagga agaaataaat
      961 gatgtttaat gacaacttaa ataaatatcg aaaacaaaag ggattgtcgc aagaggaatt
     1021 agcttttcgg ttaggagtat cgagacaatc ggtaagtaag tgggaatcag gtcaatctac
     1081 acctgaatta gaacgaataa ttgaaattgc tgatctattt ggtatatctt tagatgaatt
     1141 aattggacat gaaagtaatg attatgttac agttgaccgt gaagaattga gaagtgtagt
     1201 gcgtcatctc tttacttatg aatataagag taaatttaaa attggaaatg ttccattaat
     1261 tcatattaat ttaggtcgtg gttttagaat tgcgaaagga attattgcaa ttggcaatat
     1321 tgcggtaggg ctttttagtt ttggggcttt agcacttggt gtatttagtc ttggcggtct
     1381 ggcaattggt ttattgacat tagcaggaat tgctataggg ggaattggct taggagggct
     1441 aagtattggc tattttgctg taggtggaat ggcaatagga atctatgcaa ttggaggtat
     1501 ggcaattgcc accaaactcg caattggggg tatggctcat ggttatgttg caattggaag
     1561 ttctgcaaat ggtgttcata cattagttag cactaattgt tcactagaaa tgattagtaa
     1621 ttttatcttn nnnnnnnnna aaatagttga atttttatta ctctttatat attaaaagcc
     1681 gtctgaaaac agacgacttt tttattcatc ataaatatta acaatttctc catttaaaat
     1741 tcgattcata ttttttatag cacagaaatt accgcacata ctgcaagtat cttctttttc
     1801 aggttttgct tgttggcgtt atgtacgtgc ttttttagga tcaatggcta attcaaacat
     1861 tttttcccag tctaaatttt ttctggcaca actcatttgg tagtccaaat cttttgcccc
     1921 tttgatacct ttcgcaatat cggcagcatg tgctgcaatt ttagatgcga tgattccttc
     1981 tttaacatca tttagatcag ggagacgaag atgttcagca ggagttacat aacatagaaa
     2041 agcagcacca ttagcggcag ccaatgcacc gccaattgca gctgtgatat ggtcataacc
     2101 tggggcaata tcagtaacta gtggaccaag aacataaaaa ggggctccct ggcaaatggt
     2161 ttgttgaatt tttatattag cttcgatttg atttaaagcc atgtgacctg gaccctcaac
     2221 aattacttga acatcttttt gccatgcacg ttttgtaagt tctcccaaag tcaccaattc
     2281 ttcaatttga cttatatcgc cagcatcttc gattgaacca ggacggcaag catcacctaa
     2341 actcattgtt acatcatatt tttgacaaat atcaaggatt tcatcatagt attcataaaa
     2401 aggattttct tgaccagtca tttccatcca agcaaaaata attgaaccgc ctcgagaaac
     2461 gatattagta agacgcttat tttctttaaa acgttgagct gtatttttat taattcctac
     2521 atgaattgtc ataaaatcta ctccgtcgcg agcatgcatc tcaacaatat cgatccattg
     2581 tcgagcagta atttctttta agggcttatg ataataaact acagcatcgt agattggcac
     2641 tgttccaatt attgcgggac attctttggt taatttgcga cggaattttt gagtatcgcc
     2701 atatgaactt aaatccatta ttgattcaac tcccatattg acagcatcat tagctttttt
     2761 aagttccata tctaaatctt tccagtctct agaagttcca agatttacat taatttttgt
     2821 tttaagcatt gaaccaatcc catttacatt aatacaggta tgatacttat tggctgggat
     2881 aacaactttt ccttgggcta ttagtgtctt caattcttca atattcattt tctctttagc
     2941 cgcaacaatt ttcatttcag gagtaatgat tccctttttt gctgcattca tttgtgttgt
     3001 gtaattcatt ttttatctcc attctttttt gtatagaaaa aggtttggac caaaataggt
     3061 tcaaaccgaa cataaacaaa aatagtggcc agatccctac gttggcatta cccaaatcag
     3121 gttatacagg tcgaagttag attacttcct ctcagctcgc atacgagctc cctttctata
     3181 taaataagtc tacctttact gcacgcaaat gtcaattttt attacttatt ttgttgattt
     3241 agttttttta tatcactggt acgcccgtgt ttcaaaattc ttcgataatt ctcagctaaa
     3301 gctttttcat aattactggt catccatggt acatagaact gtgtatttcc tagttctttt
     3361 ggaagatact gaatatattc ccacaattct ggccgactat aatcatactt ttcatcatct
     3421 tctaaaccaa ccggcgtaag acgtaagtaa gaaggggttt ttaaagaacg ttcatttagt
     3481 gaagttaacg cagcatcaat tgcattttca ctagattttg attttggtga taaacataaa
     3541 tcgataattg cactagcaat aggaattcga gcttctggaa aaccgattgt tttagcggct
     3601 tgcatagcaa ttaccgtacg catacacgca tttggattgg ctaaaccaat atcttcataa
     3661 gcagttgtga taagtcgccg ttctaaagat tcaatatctt ttgattcaat cagtttagca
     3721 aaataataca tagcaccatt aggatcacta ccacggattg atttttgcaa accacttaat
     3781 gtatcgtagt aattatcttc gtctttatca tagacaacat tagttttaat agcagcagtt
     3841 ttcaaatcag cttgagtaat atgattattt tcacaaacaa ttgtacaaat ttctaaacag
     3901 ttataaccat agcgaatatc gccactacat aattttgcaa tactatgcaa tgcttctttt
     3961 tcaatcgtat atttgctgtt taaaccattt ggtgaagtca ttgccttatt taaaccagct
     4021 acaatttcat caacagttaa aggtctaact tcgagaatat gacaacgaga acgaatcgct
     4081 ggattaattg aatgaaatgg gttggctgta gtacatccag cgactattaa taatccactt
     4141 tccatgtgtg aaagaagatc atcttggcgc gctttattaa gtcgatgaac ttcatcaatg
     4201 attacaaaaa gactaccact taacttagct tcttcaataa ttgcatccat ttctttttta
     4261 tttccggttg aagcgttgaa gatgcgatat ggaatgtcaa gatcatttgc aagggccatt
     4321 gcaattgttg ttttgccaca gccggggcta ccgtataaaa taattgaaaa tggatgactt
     4381 ttttgaacaa atttagttaa aatagcattt ggtccaacta aatgttgttg tccgattata
     4441 tcttttaaat tttgaggtcg cattctattg gcaagggttg atgacatatt atcttccttt
     4501 cgtttttaaa atcagttata ataattataa catttttaaa gattgaaaga taggtggatt
     4561 aaaatgagat tagttgtaca gaaagttagt caaagtagtg ttaagattga aggagaaatt
     4621 gtaggagcta ttgataaagg atatatggtt ttagttggga taactaatgg cgatgacgag
     4681 ttattagttg aaaagatggt tgataaatta gtgaatttga gaatatttga agatgaaaat
     4741 gataaattaa atctatcttt attagatgta ggaggaagtg ttttatcaat ttcacagttc
     4801 acattatatg ctaattgtaa aaaaggacga agaccaagtt ttatcgatgc tgctaaacca
     4861 gatatttcta gtccgcttta tgattttttt aataaaaagt tagaagaaaa gggaattaat
     4921 gtggaacgag gagtatttgg agcaatgatg gaggtatcat tgattaatga tgggccagtg
     4981 acaattatct tagatagtaa tgagttattt tattaacaaa ttgagtaaac ttattgattt
     5041 tatatttttt tacaaaaaaa gacaaaaaag gctttacaag acaaaaggga tatggta
//
LOCUS       DAOMTA010000107         5082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_368_length_5082_cov_2.120549, whole genome shotgun sequence.
ACCESSION   DAOMTA010000107 DAOMTA010000000
VERSION     DAOMTA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_368_length_5082_cov_2.120549"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..703
                     /locus_tag="PLK68_04210"
     CDS             86..703
                     /locus_tag="PLK68_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89646.1"
                     /translation="MRKETEIILKDNFNYQEIILLNELANIYRSKIRNTIYHEKIWKY
                     DQSLKGLGGYACPLNVIVNPFNQFNEYRNVFRSLQYARSDMYIGSRARFVITDSGLHI
                     ESLIKILVSKNSKLKFIKNTRMLGKNISFLSDKNILEYKLCYKIKHLTNLYNLAKHDT
                     DHKNNITFDYDDGIIFYFACRKIGNELLKILDHHTYNKSYKISFK"
     assembly_gap    884..902
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<903..1391)
                     /locus_tag="PLK68_04215"
     CDS             complement(<903..1391)
                     /locus_tag="PLK68_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89647.1"
                     /translation="MNKRIFSNALEFKEQLLEIKNDFNNYFNQMSNTTYISFEDIEDD
                     KLVLYNILEEYFEVLKTHVTNKTERRFLKVVMLDINRKIDISKNFKLFDNSFSNISWI
                     VVLFVFIIVLLVSLMSFFNSLLEQFIISMVNYFLGKNTVINLKKIIENVILFVSFLIS
                     VGI"
     assembly_gap    1784..1802
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1809..3380)
                     /locus_tag="PLK68_04220"
     CDS             complement(1809..3380)
                     /locus_tag="PLK68_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89648.1"
                     /translation="MLFLYGEPPFSSSDTPSTFNPEMKCRPLSKEDIKKIMTEFANSA
                     IIAKNAGFDAIEIHGHAGYLVDQFMSPVWNKRTDEYGGSAENRMRFATEIVQSIKQAV
                     DLPIIFRIALDHLFAEGRTLDESMELIEILEKAGVDALDIDAGCYERIDYIFPTAYLG
                     DACMDYVCSEARKHVNIPIMNAGNHTPESALRLIESKDADFVMFGRQFIADPDTVNKL
                     MNDHEEDVRPCIRCNEECVGRIVGRLTKLSCAVNPQACEEERFAIKPCSQVKNIVVIG
                     AGPAGLEAARVAAAEGHNVEIYEKTNMIGGQLSVAATPKFKDQLKKLVKWFEVQLKKL
                     DVIIHFNYEVKADDQILKNADQIIPPINGINNENVISVIDAHMHKGMIKGENVIMCGG
                     GLSGIDSAIELASEHGKNVTIIEMADSIAKDVLFINQASIFAKIDEYKINVITGAKVV
                     EFNNDGITYQKDEQEVVCKADTIIRAFGMKPNRELAEEIRNTYPTKTRIVGDSEKIGK
                     VANAIRDGFYAGMSL"
     gene            complement(3340..3708)
                     /locus_tag="PLK68_04225"
     CDS             complement(3340..3708)
                     /locus_tag="PLK68_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89649.1"
                     /translation="MKYDALFTPFKIGNMEVKNRFVMAPMGTNSSHIDGCIANDEIDY
                     FEARAKGGVGLIIMGCQFLNPDLAQGSLEGVLQNSYVIPQLTAVVEATHRWGAKICCQ
                     ISCGTGRNAFPVWRTTILFK"
     gene            3840..5021
                     /locus_tag="PLK68_04230"
     CDS             3840..5021
                     /locus_tag="PLK68_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89650.1"
                     /translation="MKDMYEFLFNGLINHNLHQFMKQLYEYFHHPMVLCDVNYVVLAQ
                     HPNQQIGDMLFDHMQEHQKVAVEMLPFIQLGNYQKDLDQNNNVIYVDYGVGQTIPRII
                     AAITDNDNIIGYLCILFADGKPSSEIFSFISKLAKTIASIICHSKTGYNYNRYEYFAI
                     MNYLFSNDNINKHDLEKLLPAQPNNFSRDYCIITTKVGLKSNEIVYLNQLHQHIITLP
                     NTYSYLQDNCLYILITGLNRATKNNIINKVKSLITAVNIYKLKFGISYIFDNLLDINI
                     YKQQALKAYDLSKQQFTFFEDIALESFFTTSSYKIYLHPLITKLKQIDESTKNDYYHT
                     LKSYICNFGDSHKTIEQLAIHRNTLLYRLEKIKELTNVDLHDEKLCTLLLISFFLAEN
                     N"
BASE COUNT         1826 a          847 c          573 g         1798 t
ORIGIN      
        1 caatttactt atagactaga aaatgaaata agaaaagaat tatttgacta tatcattcaa
       61 aaatattgat tatatgaggt gacatatgcg aaaggaaaca gagattattt taaaggataa
      121 ctttaattac caagagatta tcttattaaa tgaattagct aatatttata gatcaaaaat
      181 aaggaacacg atttatcatg aaaaaatatg gaaatatgat caatcactaa aagggctagg
      241 tggttatgcc tgccctttaa acgtaatagt taatcccttt aatcaattta atgaatatag
      301 aaatgtattt agaagcttac aatacgcaag aagcgacatg tatattggct ctagagctag
      361 atttgtgata actgattcgg gactacatat tgaatctcta ataaagatat tagtctctaa
      421 aaattctaaa ctaaagttta taaaaaatac acgaatgtta ggtaaaaata ttagctttct
      481 ttcagacaaa aacatcttag agtataaact atgctataaa attaagcatc taaccaattt
      541 atataatcta gctaaacatg atacagatca taaaaataat attacctttg attatgatga
      601 tggtattata ttttattttg catgtagaaa aattggcaat gagctactaa aaatccttga
      661 ccaccataca tataataaat cctataaaat ttcttttaaa taattataag tttaaataaa
      721 ataaacacta ttttaaggtt aatgctacat agcttataaa taaagcagca gtagaaacta
      781 atactgataa aaatgtgtaa tacaactgtc tttcataatt gcttctagga aaatgcaatg
      841 aaatttcagg taaaatattt attattttat ttccaatttt cttnnnnnnn nnnnnnnnnn
      901 nnaataccaa ctgaaattaa aaaagaaaca aataaaataa cattttctat aatttttttc
      961 aaatttatta ctgtattttt acctaggaaa taattaacca tagatattat aaattgctct
     1021 aataaagaat taaaaaaact cattaagctt actaacaaaa caattataaa tacaaataaa
     1081 acaacaatcc aactaatatt tgaaaatgaa ttatcaaaca gtttaaaatt tttagaaata
     1141 tctatttttc tattaatatc taacataaca acttttaaaa atcttctctc tgttttattt
     1201 gtcacatgtg tttttaaaac ctcaaaatat tcttcaagga tattatataa aactaactta
     1261 tcatcttcta tatcctcaaa agatatataa gttgtattac tcatctgatt aaaataatta
     1321 ttaaaatcat tttttatttc tagaagctgt tctttaaact ctaaagcatt tgaaaatatt
     1381 cttttattca aattatattc tatttctaac ttgttaatta gatcattttg tatattaaca
     1441 tcacaatcat catcgcagca ttttatcata aaatcatgaa aatcaaaaaa atagtctctt
     1501 aatttttttt aatttgggga attgtccaat atattttttt tagatttgct gcatgttgat
     1561 atcttcagct ataatagaat ttacttttga ttcttgtctt cttctttttc tatccattta
     1621 gtcgtaatat ccttcgtaat tttcttcaca aatatttttt aacaatccca gtaatatctt
     1681 taccatattt ctcctacttt ctcttaaaaa taagtcatct atatacacta ttttactaaa
     1741 ttacggagct ttttgtaaac ataatataca ttaaaaaagt tcgnnnnnnn nnnnnnnnnn
     1801 nnttattttt ataaagacat tcccgcatag aatccatcac gtatagcatt agcaacctta
     1861 ccaatttttt cactatcccc aacaattctt gttttagttg gataagtatt tctaatttct
     1921 tcagctaatt cacgatttgg tttcattcca aatgctctaa taatcgtatc cgctttacaa
     1981 acaacttctt gttcatcttt ttgataagta atgccatcat tattaaactc aacaacttta
     2041 gcaccggtaa tcacattaat tttatattca tcaatctttg caaaaattga agcctggtta
     2101 ataaataata catctttagc aatactatca gccatctcaa ttattgtaac atttttacca
     2161 tgttcactag caagttcaat ggcactatca atacctgaca atcccccacc acacataatg
     2221 acattttcac ctttaatcat acctttgtgc atatgtgcgt caattacact aattacattc
     2281 tcattattga taccatttat tggaggtata atttgatctg cattttttaa aatttgatca
     2341 tctgctttta cttcataatt aaagtgtatt attacatcca atttttttaa ttgtacttca
     2401 aaccatttaa ctaatttctt taattgatcc ttaaattttg gtgtcgcagc aacacttaat
     2461 tgtccaccaa tcatattagt cttttcataa atttccacat tatgtccttc agcagcagct
     2521 actctagcag cttctaatcc agcaggtcct gcaccaatca caacaatatt ttttacttga
     2581 ctacatggct taattgcaaa acgttcttct tcacatgctt gaggatttac tgcacaactc
     2641 aacttagtaa gacgtccgac aattctccct acacattctt cattgcagcg aatacatggg
     2701 cgaacatcct cttcatgatc attcattaat ttatttactg tatctggatc cgcaataaat
     2761 tgacgaccaa acataacaaa gtcagcatct ttagattcaa ttaatcgaag tgctgattca
     2821 ggtgtatgat ttccagcatt catgattgga atattcacat gtttacgcgc ttcactacaa
     2881 acataatcca tacaagcatc acctaaatat gcagttggga aaatataatc aattcgttca
     2941 taacatcctg cgtcaatatc taaagcatct accccagctt tttctaaaat ctcaattaat
     3001 tccatacttt catccaaggt tctaccctct gcaaatagat gatctaaagc aattctaaat
     3061 ataattggta aatcaactgc ctgttttata gattgcacaa tttctgtagc aaaacgcatt
     3121 ctattttcag cactaccacc atattcatca gttcgcttat tccatacagg cgacatgaat
     3181 tgatcaacca aatagcccgc atgtccatga atttctattg catcaaaacc agcgttttta
     3241 gcaattattg cgctattagc aaactctgtc ataatttttt taatatcttc tttactcaat
     3301 ggacgacact tcatttcagg attaaatgtt gatggtgtat cacttgaaga gaatggtggt
     3361 tctccataca ggaaaagcat ttcttccagt tccacaacta atttgacaac aaatttttgc
     3421 cccccaacga tgtgtcgctt caactacagc tgtcaattga ggaataacgt aactattttg
     3481 taagactcct tctaatgatc cttgtgctaa atcaggattt aaaaattgac atcccataat
     3541 gatcaatcca accccacctt tagctcgcgc ttcaaaataa tcaatttcat cattagcaat
     3601 acatccatca atatgtgaag aattagttcc cattggtgcc ataacaaaac gatttttcac
     3661 ttccatatta cctattttaa aaggtgtaaa taatgcatcg tatttcatat aaattacctc
     3721 cgtcgatatt taaattttat ctttcttgtt aaaaaaatac catgggcaaa aatacaaata
     3781 tttaattact ggttgtgcaa attgcacaac tacatttata atataaagga ggtgagctta
     3841 tgaaagatat gtatgaattt ctattcaatg gattaataaa tcataatctg catcaattta
     3901 tgaaacaatt atacgaatat ttccatcatc caatggtctt atgtgatgtg aattatgtag
     3961 ttttggcaca acatccaaat caacaaattg gagatatgct ctttgatcac atgcaagaac
     4021 atcaaaaagt agcagttgag atgctcccat ttatccaatt aggtaattat caaaaagatt
     4081 tggatcaaaa taataatgtt atctatgtcg attacggagt tgggcagaca attccacgaa
     4141 ttattgcagc aataacagac aatgataata ttattggtta cttatgcatt ttatttgcag
     4201 atggcaagcc atcatctgaa atattcagtt ttatcagtaa attagctaaa actatcgcat
     4261 ctattatttg tcacagcaaa accggctata attataatcg ttacgaatat tttgcaataa
     4321 tgaattactt attctcaaat gacaatataa ataaacatga tttagaaaaa ttattacctg
     4381 cacaacccaa taattttagc agagattact gtattattac tacaaaggta ggcttaaaaa
     4441 gcaatgagat agtgtattta aatcaacttc atcagcatat tattacttta ccaaatacat
     4501 attcttatct ccaagataac tgcttatata ttttaattac cgggcttaat agagcaacaa
     4561 aaaacaatat tattaataaa gtaaaaagtt taataactgc tgtaaatatt tataaattaa
     4621 agtttggtat tagttatatt ttcgataatc tgttagatat taatatttat aaacaacaag
     4681 cactgaaagc ttacgattta agtaaacagc aatttacttt ttttgaagat attgctttag
     4741 aatcattttt tactactagt tcatacaaga tatatctcca tccacttatt acaaaactaa
     4801 aacaaattga tgaatcaact aagaatgatt attatcatac tttaaaatcg tatatttgta
     4861 attttggtga ctctcataaa acaattgaac aattggctat ccaccgcaat acactacttt
     4921 atcgtttaga aaagatcaag gaacttacca atgttgattt acatgatgag aaattatgca
     4981 cattgctgct aatcagtttt tttcttgctg aaaacaatta aatcattatc agcataaagt
     5041 ttgttaattt atttcaatat tttggtataa taacaattga gc
//
LOCUS       DAOMTA010000108         5080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_369_length_5080_cov_2.301095, whole genome shotgun sequence.
ACCESSION   DAOMTA010000108 DAOMTA010000000
VERSION     DAOMTA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_5080_cov_2.301095"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLK68_04235"
     CDS             <1..553
                     /locus_tag="PLK68_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM89651.1"
                     /translation="VLSIAVSFVLTIILYKRDMAKQPTQDSELPTEAKEVKTNIITAP
                     LAGKVASITEAPDEIFAQKMMGDGVVIFPTENILAAPIDGEITMIFPTKHALGIKSND
                     GIEILIHVGLDTVKLEGKPFNLFVTEGQKVKQGDKLMEIDFAMVEAAGCPIATPLVIT
                     SQNQFEIINLGTCSLNQDIIKLD"
     gene            565..1887
                     /locus_tag="PLK68_04240"
     CDS             565..1887
                     /locus_tag="PLK68_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRM89652.1"
                     /translation="MTCLNNEQIKKLEKQASNDEYRLNYHLMPPTGWLNDPNGLCQFE
                     GIYHLYYQYAPDDCFGGDKYWGHYSTKDFITFKNEPVALFPDCSLDAGGAYSGSTIIK
                     DNKMYVYYTGNVKYPGKHDYIHTGRDHNTIMVISDDGINFGEKVCLMKNDDYPDDLTL
                     HVRDPQIIKNGDHYYMILGARTNQDVGCCLLYRSDDLVNYKLVNRIISNEPFGYMWEC
                     PNLVKFDEQMILFCCPQGVETAGYDYENLYQNGYYLINGDLEKDYRLSEFIEFDHGFD
                     YYAPQLFIDEQGRTIIIGWMGMPDVPFTLPRELTFKNNKVYQLPISETKSLRKAKEII
                     NLSNHEKISCKGNIYELYLPINNRDFTLTLRNDAVIDYHSNLLTFSMGASGYGRDSRH
                     IVIDDLSNMTIYSDALTTRIYDHSSNLEISIDTEIQCTYYELNSYQIV"
     gene            2019..2984
                     /locus_tag="PLK68_04245"
     CDS             2019..2984
                     /locus_tag="PLK68_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM89653.1"
                     /translation="MEIKNNQKTIVKMMVFLCLCITLMIFGFYSYPDFKATVGQQRFG
                     ATYMTMNNPFFEVINNEIKKAVEANGDVLITLDPILDVDKQNEQILDLIDQRVDAIFV
                     NPIDAKKIEIGLKAAKKAKIPVIVVDAPIYDESLVNCSIASNNYDAGVLCAKDMMSKR
                     DHAKIILLEHATAKSAVERIQGFLDTIASNANYQVINRADCDGQIEIAMPTMKQMLKE
                     TPAVDVVMALNDRSALGALAAIESMEIDNVLVYGVDGSPDVKELINTGLIQATAAQSP
                     VKMGQLAYQKAKALLENKKIEKKIEVPVELISRDNISNFELTGWQ"
     gene            2981..4294
                     /locus_tag="PLK68_04250"
     CDS             2981..4294
                     /locus_tag="PLK68_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM89654.1"
                     /translation="MMQYSEYRSVSSIKNMMIIINFIIILFEASIILFSTKYVCNNLM
                     GRDFLDTLAYLPKNPTKVFIYSIIGFALLVMIMFIRKSENFQVRNGRVICNGLEIILC
                     FWIIYNLYMGYNGIALLVFADIIFNTKNGRNTMVIIGFILIIFLLSNYDIISNIIPMV
                     SLDSYIQVYDAATKTAILIAKNILESTNLVLFIMFLIVYIANQIRENENISKELSMIN
                     EVNKQLKDYAAVTEKIGESNERKRLAREIHDTLGHALTGIAAGIDACIAMIDIDPNVT
                     KQQLLVVSKVVREGISDVRRSLNKLRPGALEEHTLKEAIQKMIKEFSDVSEVEIMLDY
                     QLDKVDFENTKEDIIFRIVQESITNALRHGRAKKVEINIYQRVSDLEIVIADNGVGCD
                     DLKLGYGLKQMQERAAILNGRLEYYSKEGFTVKVTIPMKEGERYD"
     gene            4287..4961
                     /locus_tag="PLK68_04255"
     CDS             4287..4961
                     /locus_tag="PLK68_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM89655.1"
                     /translation="MIKVLIADDQELIRESLKIVLNTHEDLQVIDTVEDGFGVLDSLK
                     RNIPDVILMDIRMPRMDGVYCTKMVKEAYPDVKIIILTTFDDDDFVFSALKFGASGYL
                     LKGVSMDGLYQAILTVVSGGAMINPDIATKVFRLFSKMANTNYAINVNDDNVRELNKP
                     EWKVIQQVGFGLSNKEIAQKLFLSEGTVRNYLSSILSKLELRDRTQLAIWAVQTGQTT
                     KDLDDD"
BASE COUNT         1788 a          613 c          986 g         1693 t
ORIGIN      
        1 ggtcttaagt attgcagtgt catttgtttt aacaattata ttatataaac gtgatatggc
       61 taaacaacct actcaagata gtgaactgcc aactgaagct aaagaagtaa aaacaaacat
      121 tatcactgca ccgctagctg ggaaagtagc ttcaataacg gaagcaccag atgagatttt
      181 tgcacaaaaa atgatgggtg atggggtagt tatctttcct acagaaaata ttttagcagc
      241 tccgattgat ggtgaaatta caatgatctt tcctacaaag catgctttag gaattaaaag
      301 taatgatgga attgaaattc ttattcatgt aggtttggat actgtaaaac ttgaaggaaa
      361 accatttaat ttatttgtta ctgaaggtca aaaagtaaaa caaggtgata aattaatgga
      421 aattgatttt gcgatggttg aagcagcagg atgtccaatt gcgacaccat tagttattac
      481 aagtcaaaat caatttgaaa ttattaatct tggaacatgt agtttaaatc aagatataat
      541 caaattagac taggagaaat agttatgacc tgtttaaata atgaacaaat caaaaaatta
      601 gaaaaacaag caagtaatga tgaatatcgc cttaattatc atttaatgcc acctactgga
      661 tggttaaatg atcctaatgg attatgtcag tttgaaggga tttatcatct ttactatcaa
      721 tatgcaccag acgattgctt tggaggtgat aaatattggg gacattatag tacaaaggat
      781 tttattactt ttaaaaatga acctgttgcc ctgtttcctg attgttcttt agatgctgga
      841 ggtgcttatt ctggctcaac aataatcaag gataataaaa tgtatgttta ctataccggt
      901 aacgtaaaat atcctgggaa acatgattat attcatactg gtcgtgacca taatacaatt
      961 atggttataa gtgatgatgg aattaatttt ggtgaaaaag tatgtctaat gaaaaatgac
     1021 gattatccag atgacttgac tttgcatgtt cgagatccgc aaattattaa aaatggtgat
     1081 cattattata tgattttagg agctagaaca aatcaagatg ttggctgttg tttattatat
     1141 cgaagtgatg atctggttaa ttacaaacta gttaatcgca ttatttctaa tgaaccattt
     1201 ggctatatgt gggaatgccc taatctagtt aaatttgatg agcagatgat tttattttgc
     1261 tgtccgcaag gagtagaaac agcaggttat gattatgaga atctatatca gaacggttat
     1321 tatttaatca acggtgatct tgaaaaagat tatagattaa gtgaatttat tgaatttgat
     1381 catggctttg attattatgc tccacaatta tttattgatg agcaaggaag aactattatt
     1441 attggctgga tggggatgcc cgatgtccct tttacgcttc cacgtgagct aacctttaaa
     1501 aataataaag tatatcaatt accaataagt gaaactaaat cgttgagaaa agctaaagaa
     1561 ataattaatt tgtctaatca tgaaaaaata tcttgtaaag gcaatatata tgagttatat
     1621 ttaccgataa ataatcgtga ttttactcta acattacgaa atgatgcagt tattgattat
     1681 cacagtaatt tgttgacatt ttcaatgggt gcaagtggtt atggtcgtga ttcacgacat
     1741 atcgttattg atgatctatc aaatatgacg atttatagtg atgcacttac aacacgtata
     1801 tatgatcata gtagcaacct tgaaatatct attgacacag aaattcaatg tacttactat
     1861 gagctaaata gttatcagat agtttaggta tctgaagagg tcgtgactag gttacgactt
     1921 ttttattttc ttagtatgac atttgtcact ttaaaattgt gatgaaagca gttactatat
     1981 aattacaaat gtaatgtata aagaaatagg gggaatttat ggaaatcaaa aataatcaaa
     2041 aaacaattgt taaaatgatg gtatttcttt gcctctgtat tacgttgatg atttttggat
     2101 tttatagtta tccagatttt aaagcaacag taggacaaca gcgttttggg gcaacctata
     2161 tgacgatgaa taatccattt tttgaagtga ttaataatga gataaaaaaa gcggttgaag
     2221 cgaatggaga tgtcttgatt acacttgatc caattttaga tgttgataag cagaatgaac
     2281 aaatattaga tttgattgac cagcgtgttg atgcgatttt tgttaatccg attgatgcta
     2341 aaaaaataga aattggatta aaagcagcaa aaaaagctaa gattccggtt attgtagttg
     2401 atgcccctat ttatgatgag tcattagtaa attgttcaat tgcttctaac aattatgatg
     2461 caggtgtttt atgtgcaaaa gatatgatga gtaaacgaga tcatgctaag attattttat
     2521 tagagcatgc tactgcaaag tctgcggtag aacgtattca gggatttctt gatactattg
     2581 ctagtaatgc taattatcaa gttataaatc gtgctgactg tgatggtcaa attgagattg
     2641 caatgccaac gatgaaacag atgctaaaag aaactcctgc tgtagatgtt gttatggcat
     2701 taaatgatcg tagtgcttta ggagcattgg cagcgattga atcaatggaa atagataatg
     2761 ttttagttta tggagtcgat ggttcgcctg atgttaaaga attgatcaat actggactga
     2821 ttcaggcaac agcagctcaa tcacctgtca aaatgggaca gttagcttat caaaaagcga
     2881 aagcgttatt agaaaataag aagattgaaa agaaaataga agtaccagta gagttgatta
     2941 gccgtgataa tatttctaac tttgagttaa cggggtggca atgatgcaat acagtgagta
     3001 tcgctctgta tcgtcaatta aaaatatgat gatcataatc aattttatta ttattttatt
     3061 tgaagctagt attattttat tttcaacaaa atatgtttgt aataatttga tggggcgcga
     3121 ttttttagat acattagcat atttaccaaa gaatccgact aaagtattta tttattcaat
     3181 tataggattt gcattgttgg taatgattat gttcattaga aaatctgaga attttcaagt
     3241 tcgaaatgga cgagtcattt gtaatggatt agaaataatt ttatgtttct ggattattta
     3301 taatctttat atgggctata atggaatagc tttattagtg tttgcagata taatttttaa
     3361 taccaaaaat gggcgcaata caatggttat tatcgggttt attttaatta tttttttgtt
     3421 atctaactat gacattattt ctaatataat tccaatggtt tctttagaca gctatataca
     3481 ggtttatgat gccgcgacga aaactgcgat tttaatagct aaaaatattc ttgaatcaac
     3541 aaacctagtc ttgtttatta tgtttttaat tgtctatatt gcaaatcaaa ttagagaaaa
     3601 tgagaatatt tcaaaagaac tttctatgat caacgaagtt aataaacaat tgaaggatta
     3661 tgctgcggtg acagaaaaaa ttggcgagag taatgaaaga aaacgactgg cacgagaaat
     3721 tcatgataca ctcggtcatg ctttgacagg aattgcagct ggaattgatg catgtattgc
     3781 aatgattgat atcgatccta atgtaactaa acaacagctt ttagttgttt ctaaggttgt
     3841 ccgtgagggg attagtgatg tccggcggtc acttaataaa ttgcggcctg gagctttaga
     3901 agagcacaca ttaaaagaag caattcaaaa aatgattaaa gaatttagcg atgtttcaga
     3961 ggttgagatt atgcttgatt atcagttaga taaagttgat tttgaaaata ctaaagaaga
     4021 tattattttt agaattgttc aagaaagtat tacaaatgca ttaagacatg gacgagctaa
     4081 aaaggttgaa atcaatattt accaaagggt atctgattta gaaattgtta ttgcagataa
     4141 tggagttggc tgcgatgatc taaaattagg ttatgggtta aaacaaatgc aagaacgggc
     4201 tgctatttta aatggacgtt tagaatacta tagtaaagaa ggttttacag ttaaggttac
     4261 aattccaatg aaagaggggg aacgatatga ttaaggtatt aatcgctgat gatcaagaat
     4321 taattaggga atcattgaaa attgttttaa atacacacga agatttacaa gtgatcgata
     4381 cagtagaaga tggttttggt gtattagaca gtctaaaacg aaatattcca gatgttattc
     4441 taatggatat tagaatgcca agaatggatg gggtatattg cacaaagatg gttaaagaag
     4501 cctatccgga tgttaaaata attattttga caacatttga tgatgatgat tttgttttta
     4561 gtgcattaaa atttggagct agtggatacc ttttaaaagg agtatcaatg gatggattgt
     4621 atcaggctat tttaactgtt gtttctggag gtgcaatgat caatccggat attgcgacaa
     4681 aagtatttag acttttttcg aagatggcaa atactaatta tgcaatcaat gttaatgatg
     4741 ataatgttag ggaattaaat aaaccagaat ggaaagtaat tcaacaagtt ggttttggtt
     4801 tatcaaataa ggaaattgct caaaaattat ttttatctga aggaaccgtt cgaaattatt
     4861 taagctctat tttatcaaag ctagaacttc gtgatcgtac tcagttagct atctgggcag
     4921 tccaaacagg tcaaacaaca aaggatcttg acgatgatta aaaagaataa aataatgatt
     4981 attgtcttag ttttagttat gggaatcggg ttaatatatt accacaatca aaagaaagtt
     5041 ctaacaatcg gaatatttgc gggaagtaat tgggatgttc
//
LOCUS       DAOMTA010000109         5046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_375_length_5046_cov_2.438189, whole genome shotgun sequence.
ACCESSION   DAOMTA010000109 DAOMTA010000000
VERSION     DAOMTA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_5046_cov_2.438189"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLK68_04260"
     CDS             complement(<1..1055)
                     /locus_tag="PLK68_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM89656.1"
                     /translation="MRNIPIGEVLKEYGYINDEQLNVALEAQKSNRSKRLGQHLIDLG
                     FVSEYQMLEALSDKLAEPLIELSEIKVDIDAVQKIPRAMADKYNIIAIDLTDQQLTIV
                     TSDPLNFYGIEDVRLVTGMHLNVCLATKAEVSKAIDRYYNDVAALDIADDIKLNTIVV
                     EDTLDLFNESEDDTPVVKLVNTLLSRGYVNNASDIHIEPFEDKVIIRMRVDGMLVDYL
                     TLQKNIQNSLIVRIKILSNLDIAEKRLPQDGHFVGRVEGLELNMRVSVIPTVFGEKIV
                     MRYLNSNTPITRSDTYGMTLDNYNKIESMINMPHGIIYVTGPTGSGKTTTLYMLLESI
                     SKRQINISTIEDPVEKN"
     gene            complement(1073..>1324)
                     /locus_tag="PLK68_04265"
     CDS             complement(1073..>1324)
                     /locus_tag="PLK68_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRM89657.1"
                     /translation="GDGSAPGLSASSGWQIISRDWYLESAYIKDDVVYVTNGGRSIFI
                     TFNKTIDLTIDLVNGTNINGNRIDIDNPYSYFNKKNSMY"
     assembly_gap    1325..1334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1340..2527)
                     /locus_tag="PLK68_04270"
     CDS             complement(1340..2527)
                     /locus_tag="PLK68_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRM89658.1"
                     /translation="MKQFKNNNGMTLIEVTVVLLISTVLLMIVGSILVTSLNFFRDQK
                     LSDQNKQKLDGIAEYVDNEINYVTDLAIDNEAPYEREGWHYLSIIDGKLYRDGIKVFD
                     DKYYDNYTVSIKVKGYDGYRLDVKYDFNKKSELVYGTAKSYSFDNLKLKVEAGGEDYL
                     VSASNQVEVNDTYRIYYIKGINIIDTNDKPDVEEPDESNDNITVADQVHCINTYNNRG
                     VFDGVQRHYMIGDFVYYKGYWWQLITQENNYGFGAEPDREQKWKKIDKNYDRFSAYTV
                     NDVIYYPENGHYYKCLKQLDNKGGDSGYGPTGWNGIHENYWEDLGTTNPTTDSGHDCL
                     ELSTKNRIKTVMNKLDSLTKEQINKIPAYLNTVVYPVGSWVYEDVDGRKQYYLKVNAV
                     CSF"
     gene            complement(2565..3497)
                     /locus_tag="PLK68_04275"
     CDS             complement(2565..3497)
                     /locus_tag="PLK68_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM89659.1"
                     /translation="MINNKGMTLVEEIVALAIISIASLIMLVGFSTAANVFADSTRYK
                     DITNKQYAALLGNENTDKDINVSNEDAKVIIKVADKVITVKTNQSNATSKKDSQTMLS
                     KLNFNNINLSNTPQTVANCNDFLDSVLSFTTIKQFDEWIAEQGIDVASYNGWYKNNDC
                     YRYYYYNRYGAAHLTLEQDIINECNRIFDEVHQTATNQNEKKARIGDKTLYIKPYFCG
                     GIWQVGIDSEDGYFLMASELPGISGGTQWRTNLIYARGSWYYKVFAFGTSDQNSYVDV
                     AGFSNAKATVDSLFDGSISEKINLSDQSQWVKIK"
     gene            complement(3494..3958)
                     /locus_tag="PLK68_04280"
     CDS             complement(3494..3958)
                     /locus_tag="PLK68_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89660.1"
                     /translation="MIKNNKGSGLTWAVMIVMILLLIIGASLTFAYSSYNRSIDNRNQ
                     LQCDLYAKSALDSLIKAIGDNETDLIPKSSAFEDNIYPEISLPNAKGEVSQAVIHIVD
                     KEQEKDNDKKIIPLIYIEITYEYNGRTSNVQAVMQKIGGIWKVINYDGGEAA"
     gene            complement(3976..4608)
                     /locus_tag="PLK68_04285"
     CDS             complement(3976..4608)
                     /locus_tag="PLK68_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HRM89661.1"
                     /translation="MNKLSRREQILIYILVLLMVVVVGWYFMISPAMAKNTTLKQQRD
                     NLEFELESKKAVYETNIDVNAAIKKAKDELVDYHNKFMSIMNDYEIDVYFSELAAEYN
                     LTPVDLTINEASEIEIKSFSVALQEAKAESNSKSETTSNKSEEKEENPKTLVANVQQK
                     VAGSPTNIARYIGKISENTGVALTQLQYTYNRDSTKEYTILTYNLYMIEK"
     gene            complement(4601..>5046)
                     /locus_tag="PLK68_04290"
     CDS             complement(4601..>5046)
                     /locus_tag="PLK68_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89662.1"
                     /translation="ISDLEKSYKDSDYEEKYASLQEIQKTIDNLKKVNANIASYPNLS
                     EDIYNACYKAALQGKITSSNYEQTTGELTLVIETSQVPYTKQIVKNLMDLNIFTKVRY
                     SGYKEVEKEDESSNSGSIYMPSTTPEKVVVYQMTVICSLGGGINE"
BASE COUNT         1565 a          985 c          574 g         1912 t
ORIGIN      
        1 aagttttttt caactggatc ttcaatcgtt gaaatattga tttgcctttt agaaattgat
       61 tctaacaaca tatagagagt agttgttttc ccactcccag ttggtcctgt tacatatatg
      121 attccatgtg gcatattgat catactttca atcttattat aattatcaag cgtcattccg
      181 taagtatcac ttcgtgtaat tggagtattt gaatttaagt accgcataac gattttttcg
      241 ccaaatacgg taggaattac agatacacgc atatttaatt ccaatccctc tactcttcct
      301 acaaaatgac catcttgggg taatcttttt tcagcaatat caagattaga tagaattttg
      361 attcttacaa taagtgaatt ttgaatattt ttttgtagtg tcaaataatc caccaacatc
      421 ccatcgaccc gcattcttat aattacttta tcttcaaacg gttcaatgtg aatatcacta
      481 gcattattga catatcctcg agataataac gtattaacta gttttacaac aggtgtatca
      541 tcttcagatt cattgaagag atcaagtgta tcttcaacaa caatagtatt taacttaata
      601 tcatctgcaa tatctaatgc tgcaacatca ttataataac gatctatcgc tttagatacc
      661 tctgctttag ttgctaaaca aacatttaaa tgcattccag taacaagacg tacatcttca
      721 attccataga agttcaatgg atcacttgtt acaatagtta attgttgatc agttaaatca
      781 atcgcaataa tattatattt atcagccatt gcacgcggta tcttttgtac tgcatctata
      841 tcaaccttta tttcgcttaa ttcaattaac ggttctgcca acttatcgga caaagcttct
      901 aacatttgat attcacttac aaatcccaga tcaattaaat gttgacctaa gcgtttactt
      961 cgattagact tttgcgcttc taatgccaca tttaattgct catcatttat atatccatat
     1021 tcctttaata cttcaccaat tggtatattc ctcatatatt tcgtccttcc tatcaataca
     1081 tagaattctt tttattaaaa taactatatg gattatcaat atcaattcta tttccattaa
     1141 tatttgtacc attgactagg tctattgtca gatcaatagt tttattaaat gtaataaata
     1201 ttgatctacc accatttgta acataaacta catcatcttt aatatacgca ctttccaaat
     1261 accaatctct tgaaattatt tgccagccag atgatgctga aaggcctgga gcactgccat
     1321 caccnnnnnn nnnnatgtat taaaaggaac aaacagcatt tacttttaaa taatattgtt
     1381 ttcttccatc aacatcctca tatacccaac tgcctacagg atacacaact gtatttaaat
     1441 atgctggtat tttgttaatt tgttctttag tcaaactatc taatttgttc attaccgttt
     1501 ttattctatt ttttgtgctt aactctaagc agtcatgtcc actatctgta gtaggattcg
     1561 ttgttcctaa atcttcccaa taattttcat gaatcccatt ccatccagta ggtccatatc
     1621 cagaatcgcc acctttattg tctaattgtt ttaaacactt atagtaatgt ccattttccg
     1681 gataataaat tacatcatta actgtatatg cactaaatct atcgtaattt ttatcaattt
     1741 ttttccattt ttgttctcta tccggttctg caccgaaacc ataattattt tcttgagtga
     1801 ttaattgcca ccaataacct ttataataaa caaaatcacc aatcatataa tgtctttgaa
     1861 ctccatcaaa tacacctcta ttattataag tatttataca atgtacttga tcagctactg
     1921 taatattatc attactttca tcaggttctt ctacatcagg cttatcatta gtatctatta
     1981 tatttatccc cttgatataa taaatcctat acgtatcatt tacttcgacc tgatttgatg
     2041 cactaactaa ataatcttct cctccagcct caacttttaa tttcaaatta tcaaaagaat
     2101 aacttttagc agtaccatag actagctcac tttttttatt aaaatcatac tttacatcta
     2161 gacgataccc gtcatacccc ttaaccttaa ttgacacggt ataattatca tagtatttat
     2221 catcaaacac ctttattcca tcacgatata atttaccatc aattattgat aaatagtgcc
     2281 atccttctct ttcatacggt gcctcgttat cgattgctaa atctgtaaca taattaattt
     2341 cattatcaac atactcagca attccatcta atttttgttt attttgatcg cttaattttt
     2401 gatctcgaaa aaaattaagt gatgtaacaa gaatcgagcc aacgatcatc aagaggaccg
     2461 tagagatcaa taaaacaact gtgacttcaa ttaatgtcat accattatta ttcttaaatt
     2521 gtttcatctt aatctcccct tccctcttta tttctaaact atgattactt tatttttacc
     2581 cactgacttt gatcagataa atttattttt tcgctaatac taccatcaaa taaactatct
     2641 acagttgcct tagcattact gaaccctgcc acatccacat agctgttttg atcacttgta
     2701 ccaaatgcaa atactttata ataccagctg ccacgagcat aaatcaaatt agttcgccat
     2761 tgtgttccac cactaattcc tggtaactct gatgccatta aaaaatagcc atcctcgcta
     2821 tcaattccaa cttgccagat accgccacaa aaatatggtt taatatatag tgttttatca
     2881 ccgatacgag cctttttctc attttgatta gttgcagttt gatgaacctc atcaaaaatc
     2941 cgattacatt cattaataat atcctgttct aatgttaaat gcgcagcccc atatctatta
     3001 tagtaataat agcgataaca gtcgttgttt ttataccagc cattatacga tgccacatca
     3061 attccttgct ctgctatcca ttcatcaaat tgtttaatag ttgtaaacga aagtacacta
     3121 tctaaaaaat cattacaatt agctactgtt tgtggcgtat ttgaaagatt tatgttatta
     3181 aaattcaatt ttgataacat agtttgacta tcttttttac ttgtagcatt actctgattt
     3241 gtcttaacag taataacttt atcagcaact ttaataataa ccttagcgtc ttcattagaa
     3301 acattaatat ctttatcagt attctcattt cctaataagg cagcatactg tttattagtt
     3361 atatctttat accgagtact atcagcaaaa acatttgcag cagtagaaaa accaaccagc
     3421 attattaatg aggcaattga aattattgcc aaagctacta tttcttctac taacgtcatt
     3481 cctttattat ttatcatgcg gcttcacctc cgtcataatt aataactttc catataccgc
     3541 ctattttttg cattactgct tgaacattac tggtacgacc attatattca taagtaatct
     3601 caatatatat taacgggatt atctttttat cattatcctt ttcttgctct ttatctacaa
     3661 tatggataac cgcttgggat acttctcctt tagcatttgg taagctgatt tcaggataaa
     3721 tgttatcttc aaaagcacta cttttaggaa ttaaatccgt ttcgttatca ccaattgctt
     3781 ttataagtga atctaaagca ctctttgcat atagatcaca ttgtaattga tttcgattat
     3841 cgatcgaacg attataactt gaataagcaa atgttaaaga ggcccctata atcaataata
     3901 atatcattac gatcatgacc gcccatgtaa gccctgagcc tttattattc ttaatcatat
     3961 ttagcctcct tcatatcatt tttcaatcat atataaatta tatgttaaaa ttgtatattc
     4021 tttagtacta tccctattat aagtgtactg taattgagtt aatgcaactc ctgtattttc
     4081 agatatctta cctatataac gagcaatgtt tgtagggctt cctgcaacct tttgctgcac
     4141 atttgcaact aacgttttag ggttttcttc tttttcttcg ctcttatttg aagttgtttc
     4201 gcttttactg ttactttcag cttttgcttc ttgtaaggcc acgctaaaag atttaatttc
     4261 tatttcacta gcctcattga ttgttaaatc aactggtgtt aggttatact cagctgccaa
     4321 ttctgaaaaa taaacatcta tttcataatc attcataatc gacataaatt tattatgata
     4381 atcgactaac tcatctttcg cttttttaat tgcagcatta acatctatat tagtttcata
     4441 taccgccttt ttactttcca actcaaattc aagattatcc cgctgttgtt ttaatgttgt
     4501 attttttgcc atagcaggag aaatcataaa ataccagccc acaacaacta ccattagtaa
     4561 tactaagata taaatcaaga tttgttcacg tctacttaat ttattcattg atccctcctc
     4621 ctaaactaca gataactgtc atttgataaa caactacctt ttccggggtt gtactaggca
     4681 tatagataga accagaatta ctagattcat cttctttttc cacttcttta taaccactat
     4741 atcttacttt tgtaaaaata tttagatcca tcaggttttt aacaatttgt tttgtgtatg
     4801 gaacttgact tgtttcaata accaaggtta gttccccggt tgtttgttca taattacttg
     4861 aagtaatttt gccttgtaaa gccgctttat aacaagcatt ataaatatct tcacttaagt
     4921 taggataaga agcaatatta gcattaactt tttttagatt atcaattgtt ttttgaattt
     4981 cttgcaatga agcatatttt tcttcataat cactatcttt ataacttttt tctaaatcac
     5041 ttattt
//
LOCUS       DAOMTA010000110         5039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_378_length_5039_cov_2.209872, whole genome shotgun sequence.
ACCESSION   DAOMTA010000110 DAOMTA010000000
VERSION     DAOMTA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_378_length_5039_cov_2.209872"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLK68_04295"
     CDS             <1..205
                     /locus_tag="PLK68_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89663.1"
                     /translation="TVLPYSLQTLEAAMHQEELPKSYFTFVTILAAQMGVGGDDSWGA
                     PILEEYCIKGEEDVEYAFEICKR"
     gene            317..>1146
                     /gene="pyk"
                     /locus_tag="PLK68_04300"
     CDS             317..>1146
                     /gene="pyk"
                     /locus_tag="PLK68_04300"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM89664.1"
                     /translation="MLKNEVMSKTKIVCTIGPASDNKEMLTKLVKAGMNVMRLNFSHG
                     THPEHQAKIDLITEINKELDTSVAILLDTKGPEIRTGDFIDGSTEFKKGQVVTICQED
                     IVGTSDRFTITYKELYKDVKPGGFILVNDGQVELLVDHVEGTDIVCVCANNGIVKNKR
                     GINVPGIKLGFDYLSPKDIDDLTYGCTQPFNYVAASFVRRAQDVFDVKKLLVENGRPD
                     IQIIAKIENSEGVENIDEILKIADGIMVARGDLGVEVPAEDVPLIQKEVITKCKDMGK
                     "
     assembly_gap    1147..1156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1157..1747
                     /locus_tag="PLK68_04305"
     CDS             <1157..1747
                     /locus_tag="PLK68_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM89665.1"
                     /translation="ATQMLESMQQNPRPTRAEVSDVANAIFDGTDAIMLSGESASGLY
                     PQEAVMTMSKIALKTENSLDYDALHRQAVRTAPQDTSEAICMSVAEIASKFQVAAIIA
                     FTESGFTARKMSRYRPEARIIAATPEVATTRALAINWGVKPVKCKTMKTRSSMMDYAE
                     IIAKENGVESGELILVTGGKPGLKGDTSYLELVRVK"
     gene            1800..2435
                     /locus_tag="PLK68_04310"
     CDS             1800..2435
                     /locus_tag="PLK68_04310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM89666.1"
                     /translation="MLLKDKMKNGQLYREFGHENIEDQEYEKEIERQRLNCKARMFEY
                     NHCHPDNKKEKQDILRGLLGHAGENIWIEAPAYFAYGCNTYIGENFYANFNLVVVDDI
                     EVHIGNNVMVAPNVTLSVTGHPVDPEYRRGGTQFSLPIVIGDDVWIGANSVILPGVTI
                     GDNSVIGAGSVVTQDIPANSVAYGVPCRVIREINDYDKEYYRKGKKVNLDW"
     gene            2491..2754
                     /locus_tag="PLK68_04315"
     CDS             2491..2754
                     /locus_tag="PLK68_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89667.1"
                     /translation="MDPLQDEYYMIDNQLDSRQPIIPFWFWWLFPPPWQPGPPWQPGP
                     PGPGPRPPWQPGPPGPGPRPPWQPGPPGPGPRPPGLPPRPPRW"
     gene            2872..3777
                     /locus_tag="PLK68_04320"
     CDS             2872..3777
                     /locus_tag="PLK68_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89668.1"
                     /translation="MNYLTLDIGGSSIKYALLNETGEFIEKGSTIAPHQSIEQFVEVI
                     GELYDKYAEQIVGMAISMPGAIDPQKGFAYTGGAYKYIKDMEIVKILQERCPLPITIG
                     NDAKCAANAEVGFGCLKDVDDAAVIILGTGIGGCIVRDGKVHIGKHFSSGEFSWMRTN
                     GEDGNNPECVWAATNGIPGLLKAVQESVGTKEQYNGKEIFEMANSGDKKVLAGLDKFC
                     NRLAVQIYNLQALFDPEKIAIGGGISAQPLLLELVEKHIEEMYQTGLKANSPIARPVV
                     VPCQYRNDANLLGAFYQHLHTCKKN"
     gene            complement(3907..>5039)
                     /locus_tag="PLK68_04325"
     CDS             complement(3907..>5039)
                     /locus_tag="PLK68_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM89669.1"
                     /translation="ESIFTILAKKAALRRENGKEVIDFSTGTPNIPPTKRIREVLANA
                     ALDPKNYVYAINDLPELIKAVIAWYKKRYEVILEDDEICSLLGSQEGLAHLAMTLIND
                     SDIVLVPNPSYPIFKDGPLLANANIVDMPLKEENNYLIDFDKIDPTIAKKAKFMIVSY
                     PNNPTCAIANDKFYLRLIDFAKKYQIIVLHDNAYSELLFEGLGRSFLSYPGAKDVGIE
                     FNSLSKTYGLAGARIGFAVGNKAIIEQLKTLKSNMDYGMFIPIQLAAIEAITGEQNCV
                     IKTRESYQRRRDIFLKCAQEIGWNIKETKGTMFIWAKIPSLYQKSIDFVNDLFEQTGI
                     LFIPGSAFGSEGEGYIRIALIQDEEIIKKAFIKMKATNIFKN"
BASE COUNT         1682 a          756 c         1009 g         1582 t
ORIGIN      
        1 aactgttctt ccatatagct tgcagacttt agaagctgca atgcatcaag aagaattacc
       61 aaaatcatat tttacctttg taactatttt agctgctcaa atgggtgttg gcggtgatga
      121 tagttggggt gctccaatac tcgaagaata ctgtattaaa ggtgaagaag atgttgaata
      181 cgctttcgaa atttgtaagc gttaatcaac taaaaatctt ttgataaatg ttatcaaaag
      241 tctcgcaagt gttttttaga tgggctataa taaacatgta tgataaattg agaaggaaaa
      301 ggatggtaat gacgatatgc taaaaaacga agtaatgagt aaaacaaaaa ttgtatgtac
      361 gataggacca gcatcagata acaaagagat gttaactaaa ttagttaaag ctggaatgaa
      421 tgtaatgaga cttaattttt ctcatggaac tcatcctgag catcaggcaa aaattgattt
      481 gattactgaa atcaataagg agttggatac aagcgttgct attttattag atactaaagg
      541 gccggaaatt agaacaggtg attttatcga tggttcaact gaatttaaaa aaggtcaggt
      601 tgtcacaatt tgtcaagaag atattgtcgg aacaagtgat cgttttacaa tcacttataa
      661 agaattatat aaggatgtaa aacctggtgg gtttattttg gtaaatgacg gtcaagtgga
      721 attgctagtt gatcatgttg aaggtacaga cattgtatgt gtttgtgcta ataacggaat
      781 tgtaaaaaat aaaaggggaa tcaatgtacc gggaattaaa ttaggtttcg attatctatc
      841 accaaaagat attgatgatt taacttatgg atgtacacaa ccattcaatt atgtggctgc
      901 ttcttttgta agacgtgcac aagatgtatt tgatgtaaaa aaacttttgg ttgaaaatgg
      961 tcgacctgat attcaaatca ttgctaagat tgaaaatagc gaaggtgttg aaaacataga
     1021 tgagattcta aaaatagctg atgggattat ggttgctcgt ggtgatttag gcgttgaagt
     1081 acctgcagag gatgtcccat tgattcaaaa ggaagtcatt acgaaatgta aggatatggg
     1141 aaaattnnnn nnnnnngcta ctcaaatgct tgaaagtatg cagcaaaatc ctcgcccgac
     1201 tcgtgcagaa gtaagtgatg ttgccaatgc tatttttgat ggaacagatg caatcatgct
     1261 atctggggaa tctgcatcag gcctatatcc acaagaagca gtaatgacaa tgagtaagat
     1321 tgctttaaaa acagaaaata gcttagatta tgatgcatta catcgtcaag ctgtaagaac
     1381 tgctccacaa gatacatctg aagctatttg tatgtcagtt gcagaaattg cctctaaatt
     1441 ccaagttgca gcgattattg cttttactga aagtggattt actgctcgaa aaatgtctcg
     1501 ttatcgtcct gaagctcgga tcattgctgc tacacctgaa gtagcaacta ctagagccct
     1561 tgcaattaac tggggagtaa aaccagtaaa atgcaaaaca atgaaaacac gttcaagtat
     1621 gatggattat gctgaaatca ttgcaaaaga aaatggtgtt gaatcgggag aacttatttt
     1681 agttactggt ggtaaaccag gacttaaagg ggatactagc tatttagagt tagttcgtgt
     1741 taaataatat taaggatatg acacccagct tagctgggtg tttatcataa ggaggatata
     1801 tgttattaaa agataaaatg aagaacggtc aattatatcg ggaatttggc catgaaaata
     1861 tagaagatca agagtatgaa aaagaaattg aacgtcaaag attaaattgt aaagcaagaa
     1921 tgttcgagta taatcattgc catcctgata ataaaaaaga aaaacaggat attttaagag
     1981 ggttactagg ccatgctggt gaaaatatat ggattgaagc tcccgcttat tttgcctacg
     2041 gctgtaatac ctatattggt gaaaattttt atgctaattt taatttggtt gttgttgatg
     2101 atattgaagt tcatattggt aacaatgtaa tggttgctcc aaatgttaca ttatcagtaa
     2161 ctggtcatcc tgttgatccg gaatatcgaa gaggtggaac gcaattttca ttgccgattg
     2221 taattgggga tgatgtttgg atcggtgcaa atagtgttat tttaccgggg gttacgattg
     2281 gtgacaatag tgttattgga gctggtagtg ttgtaaccca agatattcca gcaaattctg
     2341 ttgcttatgg ggtaccatgc cgagtaattc gcgaaattaa tgattatgat aaagaatatt
     2401 atcgtaaagg aaaaaaagtt aatcttgatt ggtaaaagcc catgccttct ttaaagggat
     2461 acatattgta taataaagtg aggtgtttaa atggatccgc ttcaagatga atattacatg
     2521 attgataatc agttagatag tcggcagcca attattccat tttggttttg gtggttattt
     2581 cctccaccat ggcaaccggg accaccatgg cagccaggcc caccgggacc gggaccacga
     2641 cccccatggc agccaggccc accaggaccg ggaccacgac caccatggca gccaggtccc
     2701 ccaggaccag ggccacgacc cccaggactg ccaccacgtc cgccacgatg gtgaaatagt
     2761 aaaaaatagg tacacaaatg tgtatctatt ttcgtgtttt gataatttat tgaatcttgt
     2821 tggaaatatc gttatactta aaagtaagag cgaaatatga aaggagataa tatgaattat
     2881 ttaacattag atattggtgg tagttcaatc aagtatgctt tattaaatga aacgggagag
     2941 tttattgaaa aaggaagtac aattgcacct catcaaagta ttgagcagtt tgttgaggta
     3001 attggagaac tgtatgataa gtatgctgaa caaattgttg gtatggcaat ttctatgccg
     3061 ggtgcaatag atcctcaaaa gggctttgct tatactggtg gtgcatataa atatataaaa
     3121 gatatggaaa ttgtaaaaat tttacaagaa cgttgcccgt tgcctattac gattggtaat
     3181 gatgctaaat gtgcggctaa tgcggaagta ggatttggat gtttaaaaga tgttgatgat
     3241 gctgctgtaa taattttagg aactggaata ggcggttgta ttgttcgtga tggtaaagtt
     3301 catattggta aacatttctc ttcaggagaa ttttcatgga tgcggactaa tggggaagat
     3361 ggcaataacc ctgaatgtgt atgggcagct acgaatggaa ttcctggctt attaaaggca
     3421 gttcaagagt ctgttggaac aaaagaacaa tataatggta aagagatttt tgaaatggca
     3481 aatagtggag ataaaaaggt acttgctggg cttgataaat tctgtaatcg tttagctgtt
     3541 caaatttata atttacaagc actttttgac ccagaaaaaa ttgcgattgg tggtggtatc
     3601 tcagcacaac cattattatt agaattagtt gagaaacata tagaagaaat gtatcaaact
     3661 ggattaaagg ctaattcacc aattgcacgc cctgtagttg ttccttgtca atatcgtaat
     3721 gatgctaatc tattaggcgc tttttatcaa catttacata catgtaagaa aaattaacat
     3781 aatgaagcaa ggcccaaacg ggtttgcttt tctattatat ctataaataa tgataaaagt
     3841 agtttgagca ataaaacctc tttttgattc taaccaaaaa gaggtttgct aattgatatt
     3901 taaaatttaa tttttgaaaa tattagttgc tttcatttta atgaaagcct tttttattat
     3961 ttcttcatct tgaattaaag caattctaat atatccttct ccttcgctgc cgaaagcact
     4021 accaggaata aataaaatac cggtttgctc aaataaatca ttaacaaaat caattgattt
     4081 ttgatataag ctaggaattt ttgcccaaat aaacatagtt ccctttgtct ctttaatatt
     4141 ccagccaatt tcttgagcac attttaaaaa gatatcgcga cgccgctgat aagactctct
     4201 tgtttttata acacaatttt gttcaccggt aatggcttcg attgctgcta attgaatagg
     4261 gataaacatg ccataatcca tattagattt taatgtttta agctgttcaa taattgcttt
     4321 gttgccaact gcaaatccaa ttcgagcacc agcaagccca taagtttttg ataaggaatt
     4381 aaattcaata cctacatctt tagcgccagg atatgataaa aagcttctac ctaagccttc
     4441 aaataatagt tcgctataag cattatcgtg taatacaata atttgatact ttttagcaaa
     4501 gtcaattaat cttaaataaa acttatcatt tgcaatcgca caggtagggt tgttaggata
     4561 tgaaacaatc ataaattttg cttttttagc aatggttgga tcaattttat caaagtcaat
     4621 caaataatta ttttcttctt ttaaaggcat atcgacaata tttgcattag caagcaacgg
     4681 cccgtcttta aaaataggat agctaggatt aggaactaag acaatgtcac tatcgttgat
     4741 caatgtcatt gctagatgtg ctaagccttc ctgagaaccc agtaatgaac aaatttcatc
     4801 atcttctaaa atgacctcat aacgtttttt ataccaagcg attacagcct tgattaattc
     4861 tggtaaatca ttaatcgcat aaacatagtt tttaggatct agggcagcat tagcaagcac
     4921 ttctcttatt cttttggtag ggggaatatt tggcgtaccc gtactaaaat caattacttc
     4981 ttttccattt tcacgtctta atgctgcctt ttttgctaaa attgtaaaaa tactttctt
//
LOCUS       DAOMTA010000111         5033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_379_length_5033_cov_2.470671, whole genome shotgun sequence.
ACCESSION   DAOMTA010000111 DAOMTA010000000
VERSION     DAOMTA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_5033_cov_2.470671"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..587)
                     /gene="rlmH"
                     /locus_tag="PLK68_04330"
     CDS             complement(108..587)
                     /gene="rlmH"
                     /locus_tag="PLK68_04330"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRM89670.1"
                     /translation="MKIKIVCVGRLKEKYLVAGMQEYLKRLQAYCKVEVYEVADESIP
                     DNCSLANETLIKAKEGRKMLDKIKQDDYVILLDVSGTQIDSVELSNKMEKAMISGKST
                     IAFVIGGSLGHGEEMLDRANFRLSFSKMTLPHQLMRLVLVEQIYRAFKIMKNETYHK"
     gene            complement(577..1326)
                     /locus_tag="PLK68_04335"
     CDS             complement(577..1326)
                     /locus_tag="PLK68_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM89671.1"
                     /translation="MEFHILASGSKGNSTFIYDNGVGILIDCGIARKQLLYKLGNLGF
                     QESNINYVLLTHDHYDHNKNISIFDQRICFCGKGCIKGIDSSHEIKPYKSFQLAHFEI
                     MPLSISHDATSPLAFIITGVNESILYMTDTGYVSQKNRKYLNNLDYYIIESNHDVEML
                     MATKRPYFLKQRIHGDLGHLNNEYSAKMMVELIGDKTKEIVLAHLSEEANTKEKALET
                     YRKIFNQNNLEFDNIKVASQIDVVSGGNYED"
     gene            1469..2053
                     /locus_tag="PLK68_04340"
     CDS             1469..2053
                     /locus_tag="PLK68_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM89672.1"
                     /translation="MPRIITIAREYGSGGRLIAQKVAQKVGLVYYDNEVIDLAAREMG
                     MDVDAIRKVAEQKSSSFMYTMSSSAFSLPLNDQVFVMQSKIIRHLANHDSCIIVNGCA
                     DYILEDYDDVLSIFIHAPLESRIRRVKEDYQEVHDDYKKYVTKRDKGRSNYYNYYTTK
                     KWGHLKNFDLTINSDLGIDEVATIVADLFLKGEK"
     gene            2053..>2877
                     /locus_tag="PLK68_04345"
     CDS             2053..>2877
                     /locus_tag="PLK68_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89673.1"
                     /translation="MTQENKMGTMPVNKLLISMSLPMIISMLVQAMYNIVDSVFVAQI
                     SENALTAVSLAFPLQNLMIAFAGGTAVGVNALLSRSLGEKNQDHVNQTAVNSVFIFLV
                     TAVIFMIAGLTLSNLFFNVQTTNTEIVNAGTQYSMIVVGCSIGLFCQFLFERLLQATG
                     RTLFTMVTQGLGAIINIILDPIFIFGLCGFPKMGVAGAALATITGQIIACLLALFFNL
                     KFNHDIHFKFKRFRPNAHIVKQIYSVGIPSIIMQSIGSVMTFGMNTILITFSTTATA"
     assembly_gap    2878..2897
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2898..3400
                     /locus_tag="PLK68_04350"
     CDS             <2898..3400
                     /locus_tag="PLK68_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89674.1"
                     /translation="FKLQSFVFMPVFGLNNGMIPIIAYNLGAKQKKRMFDTIKLAMIY
                     ATGMMIIGVIFFETIPQTLLGFFNASEAMIKIGTPALRIIAIHFIFAGFSIVCSATFQ
                     AVGKGTYSLLTSLIRQLLVLLPCAYVLSLTGNLDLIWLCFPIAEIFSAVTSFILMKRT
                     RRHLEF"
     gene            complement(3467..3670)
                     /gene="rpmE"
                     /locus_tag="PLK68_04355"
     CDS             complement(3467..3670)
                     /gene="rpmE"
                     /locus_tag="PLK68_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRM89675.1"
                     /translation="MKKGIHPDYKKVKIVCTSCGNEFEGGSVLDEVRVDTCSNCHPFY
                     TGKQRFASADGRVDKFNKKYGLK"
     gene            complement(3777..>4355)
                     /locus_tag="PLK68_04360"
     CDS             complement(3777..>4355)
                     /locus_tag="PLK68_04360"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610686.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM89676.1"
                     /translation="IPDRIEAGTFLVMAAAAGEKVIIQNVIPQHLEAVTSKLKEIGVK
                     MDIVGDSIIVHGGLDNLKPIDIRTQVYPGFATDLQQPLTALLTQCQGASQVVETIYPE
                     RFGHCYQLNSMGAKIDQEEAMCYINGPTPLHGARVYATDLRCGAALIVAALMADGVTE
                     IGNVYHIDRGYENIDHKLISLGAVITRRQVED"
     assembly_gap    4356..4365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4366..5032)
                     /locus_tag="PLK68_04365"
     CDS             complement(<4366..5032)
                     /locus_tag="PLK68_04365"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610686.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM89677.1"
                     /translation="MEGGHKLNGTVVVSGAKNATVALIPAIVLADSPVTVYGVPNISD
                     VDALGVLLRELNCEVDKNDDVLVVDPSNLKNIPLVSDAVDKLRASYYLMGALLGKCKK
                     VTMKMPGGCFLGPRPIDLHIKGFEALGAKVSYSDNGTYTIEADRLVGNKIYLDFASVG
                     ATINILLAAVKAEGKTTIENSAKEPEIIDVVNLLTKMGAKIRGAGTDTITIEGVETLC
                     GCDH"
BASE COUNT         1549 a          955 c          729 g         1770 t
ORIGIN      
        1 ccagttcttc tttgactctt gtatagattt cttcgaagcc atcatctaaa aatacgattt
       61 tatcaatata ctggcttttt tcatctatat tcttttttat taatacctta tttatgatat
      121 gtttcgtttt tcataatttt aaatgcccga tagatttgtt ctactaagac tagcctcatc
      181 aattgatgcg gtaaagtcat cttagaaaaa cttagcctaa aattagcacg atcaagcatt
      241 tcttcaccat gaccaagtga accaccaatt acaaatgcaa tcgtactttt accagaaatc
      301 atggcttttt ccatcttatt tgataattca acagaatcta tttgcgttcc cgacacatct
      361 agcaaaataa cataatcatc ctgcttaatt ttatcaagca tcttacgccc ttctttagct
      421 ttaattaatg tttcattggc cagtgagcag ttatccggta tactttcatc tgctacttca
      481 taaacttcta ctttgcaata ggcctgcaat ctctttaaat attcctgcat tcctgccact
      541 aaatattttt cttttaaacg cccaacacaa actattttaa tcttcataat tacctccgct
      601 gaccacatca atttggctag ctactttaat attatcgaac tctagattat tttgattaaa
      661 aatcttacga taggtttcta atgccttctc tttagtatta gcttcctcac tcaaatgcgc
      721 taaaacaatt tctttagttt tatcaccgat taactctacc atcatctttg cactatattc
      781 attgtttaaa tggcctagat caccatgaat acgctgtttt aaaaaataag gtcgtttcgt
      841 tgccatcagc atctcaacat catgattact ttcaataata taatagtcta gattattaag
      901 atatttccga tttttttgag atacataacc agtatctgtc atatacaaaa tagattcatt
      961 cacaccagta atgataaaag ccagcggact tgtcgcatca tgagatatac ttaaaggcat
     1021 gatttcaaaa tgagctaatt gaaaactttt atatggcttg atttcatggc tgctatcgat
     1081 tcctttgata catcctttac cacaaaaaca gattctttga tcaaatatac taatattttt
     1141 attatggtca taatgatcgt gtgttaataa aacataatta atattacttt cttgaaaacc
     1201 aagattgcca agtttatata ataattgttt tctagcaatc ccacaatcaa tcaatattcc
     1261 cacaccatta tcataaataa aggtactgtt tccttttgaa ccacttgcta aaatatgaaa
     1321 ttccattgtc ttctcctttt attgttttat tatacaaact tgtatgcaca aaaacaataa
     1381 atttgtcttg gaaaaagcag gatgtgaaaa tattgtcatt aattttaaat tttatgataa
     1441 aataagaata taaaggggga ttttcaatat gcctagaatt attactattg ccagagaata
     1501 cggttctggc ggacgcttaa ttgcacaaaa agttgcgcag aaagtcggac ttgtatacta
     1561 tgataatgaa gtcatcgact tggcagctcg cgaaatggga atggatgttg atgcaatccg
     1621 taaagtagct gaacaaaaat catctagctt tatgtataca atgtcatctt ccgctttttc
     1681 attaccacta aatgatcagg tttttgtaat gcagtctaaa atcattcgtc atttagctaa
     1741 tcatgattca tgtatcattg ttaatggatg tgctgattat atccttgagg attatgatga
     1801 tgttttatct atttttattc atgcaccact agaatcacga attcgccgag ttaaagagga
     1861 ttatcaagaa gtacatgatg attataaaaa atacgttact aaaagagaca aaggtcggag
     1921 taattattac aattactata caacaaaaaa atggggtcat ttaaaaaatt tcgatttaac
     1981 gattaacagt gatttaggaa tcgacgaagt cgcaacgatc gttgctgatt tgtttttaaa
     2041 aggagaaaaa tagtgaccca agaaaataag atgggaacaa tgccggtcaa caaactgcta
     2101 atttcaatgt ctctaccaat gattatctca atgttagttc aagcaatgta taatatcgtt
     2161 gatagtgtct ttgttgccca aataagcgaa aacgctttaa ctgctgtttc tttagcattt
     2221 ccattgcaaa acttgatgat tgcctttgcc gggggaactg cagttggcgt caatgcttta
     2281 ttgtcacgta gtttaggtga gaagaatcaa gatcatgtta accagacagc agtcaattca
     2341 gtatttattt ttcttgtcac ggcggttata tttatgatcg ccggactaac attgtctaac
     2401 ctctttttta atgttcaaac aactaatact gaaattgtta atgccggtac ccaatattca
     2461 atgatcgtag ttggctgttc aattggattg ttttgtcaat ttctatttga acgattatta
     2521 caggcaactg gaagaaccct gtttactatg gttactcagg gattaggtgc cataatcaat
     2581 ataattttag accctatttt tatttttggt ttatgtggct ttcctaaaat gggtgtcgct
     2641 ggtgctgcac ttgcgacaat aaccggtcaa atcattgctt gtcttttagc tctatttttc
     2701 aatcttaaat ttaatcatga tattcatttt aaatttaaaa ggtttcgacc taatgctcat
     2761 attgttaagc aaatatattc agtaggaatt ccatctatca tcatgcagtc tatcggtagt
     2821 gtcatgactt ttggtatgaa tactatctta attacttttt ctaccacggc tactgcgnnn
     2881 nnnnnnnnnn nnnnnnnact tcaaattaca aagctttgtc tttatgcctg ttttcggtct
     2941 taataacggt atgattccaa tcattgccta taatcttggg gctaaacaaa agaaacgaat
     3001 gtttgataca ataaaattgg caatgatcta cgctactggc atgatgatca ttggtgttat
     3061 cttctttgaa acaattccgc aaactctttt aggtttcttt aacgcctctg aggcaatgat
     3121 aaaaattgga acaccggctt taagaataat tgctattcat ttcatttttg caggtttctc
     3181 aattgtttgt agtgcaactt tccaagctgt tggtaaaggt acatatagtc ttttaacttc
     3241 gttgatcaga caattattag tattattacc ttgtgcttat gtattatcat taacaggtaa
     3301 cttagactta atttggctat gtttcccgat tgcggaaatt ttctctgcag tgacttcttt
     3361 tattttaatg aagcgaaccc gtagacattt agaattttaa gacttatgaa tcttcataag
     3421 tcttttttgc agcaaaaaag gagcttgcgc tcctttactt gtgtaattat ttaagtccgt
     3481 attttttatt gaatttatca acacgtccat cagcactagc aaatctttgt ttaccagtat
     3541 agaatggatg acagtttgaa caagtatcaa cacgtacttc gtctaaaact gatccacctt
     3601 caaattcgtt tccacaagaa gtacaaacga tttttacttt tttgtaatct ggatgaattc
     3661 cttttttcat aaatatcaat ctccttccgc cttatagcat atagctacaa agtaagttgc
     3721 tttagtataa taacattaaa atatctagat ttcaagtcat ttttttaaat taatcactaa
     3781 tcttcgactt gacgtctagt aatcacagct ccaagtgaaa ttaacttatg atcaatattt
     3841 tcatagccgc gatcaatatg ataaacattt ccaatttcag taacaccatc agccattaaa
     3901 gctgccacga tcaacgctgc accacaacgt agatctgtcg cataaacacg tgctccatgt
     3961 aatggcgtag gaccattaat ataacacatt gcttcttctt gatcaatttt tgctcccatt
     4021 gaattcagct gataacaatg tccaaagcgt tcaggataaa tcgtttcaac tacttgtgaa
     4081 gctccctgac attgagttaa taacgctgtt agcggttgtt gtaaatcggt tgcaaaacca
     4141 ggatatacct gtgtcctaat atcaattggt tttaaattat caaggccgcc atgaacaata
     4201 atactgtcac caacaatatc cattttaacc ccgatttctt ttagtttaga agttactgct
     4261 tctaaatgtt gaggtataac attttgaata attacttttt ctcctgccgc tgcagccatc
     4321 actaaaaatg tcccagcttc aattcggtca ggaatnnnnn nnnnncatga tcacaaccac
     4381 aaagagtttc tacaccttca attgtgatcg tatctgttcc agccccacga atcttagctc
     4441 ccattttcgt aagtaaatta acaacatcaa taatttcagg ttcctttgca gaattttcta
     4501 ttgttgtttt tccttctgct ttaaccgctg ccaacaggat attgattgtt gctcctactg
     4561 aagcaaaatc caaataaatt ttattcccaa ctaaacgatc agcttcaatc gtataggttc
     4621 cattatcact ataactaact tttgcaccta atgcttcaaa tcctttaata tgtaaatcga
     4681 ttggtcgtgg tcctaaaaaa cacccaccag gcatcttcat cgtcactttt ttgcattttc
     4741 ctaataaagc tcccattaaa taataagatg ctcttaattt atcaactgca tcacttacta
     4801 aaggtatatt ttttaagtta gaaggatcaa ccactaacac atcatcattt ttatctactt
     4861 cacagtttaa ttctcttaac aatactccta gtgcatcgac atcagaaata tttggtaccc
     4921 catatactgt tacaggacta tctgctaaga caatcgcagg aattagagct accgttgcat
     4981 tttttgcacc gcttaccacc acagttccat tcaatttgtg tccaccttca atg
//
LOCUS       DAOMTA010000112         5022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_382_length_5022_cov_2.587477, whole genome shotgun sequence.
ACCESSION   DAOMTA010000112 DAOMTA010000000
VERSION     DAOMTA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_5022_cov_2.587477"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..2241)
                     /locus_tag="PLK68_04370"
     CDS             complement(85..2241)
                     /locus_tag="PLK68_04370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM89678.1"
                     /translation="MGESNFTESDRKIVYVKDEITGIKVQYLKVVFSNSLEDDVFRVY
                     VNSEEIDFNKKIISDDGEIQIEVAIPKDCKQLYITCNNDESIERIYTLDISLVFKFKI
                     TLLKLVSIIRRSIIVFGRGMRFLWREHHMIIPPSLWKKYFLRFFMRVKERGEILWNPF
                     IQDDYHKWINIFENDEHIEVFEYNPLMSILIPVYNVEKKYLVECLESILNQTYQNFEV
                     CLVDDCSTNEETIETIKEYENKDSRIRVNRRKRNGHISRASNDALDMAKGEFVCLVDN
                     DDMLAANALYENVALLNKYENVDFIYSDEDKLDIRGSRCEPHFKSDFAPDTLLGINYI
                     CHFAVLRTSLVRYLGGFTVGLEGVQDHDLFLRVSEQTKNIYHIPKILYHWRMVEGSTS
                     MAIDNKGYAVNKGIRAVEEALKRRNVSANVQSIGGSTVYRVEYNYKEEPSVSIIIPTR
                     DFADITENCLKSIYELTEYTNYEVVVVDNNSVNQETFDLFKRYQEANTNFRVVKADMD
                     FNYSAINNLAVDTCISDVIILLNNDTEVITPNWLKLLVSYAIQDHIGAVGSKLLYPDM
                     TIQHGGVLLGLGNAVAAHAFISHPRDDEGVYGRLKIPYNYSAVTAACLAVERKKYLQV
                     NGLEEKLKVAYNDIDFNLKLLDAGYYNLFIPQVELIHYESKTRGLDSTSEKYKQFVSE
                     NNFMYEKWSSYIENDPYYNSNFSRMGVYMLDREKRR"
     gene            complement(2243..3025)
                     /locus_tag="PLK68_04375"
     CDS             complement(2243..3025)
                     /locus_tag="PLK68_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM89679.1"
                     /translation="MKLIRNLYQYRELLKSNIKKEIRGKYKGSMLGVLWSFLNPLLMV
                     MVYFLVFPFLFRDTVDNYLVYLVIGVIPWTFFTTTMNQGIMTIRVNEGIIKKVYFPRE
                     ILPISVATSGLVNFFISSIIMILFCLFGGVGLSWHIILFPVFAIVQYFIIMGLVLGLS
                     AINVYVKDTEYIIQFFVNMLFYGTPILYDLSTFDKFPTLLMKIINLNPFKHLMVIYRD
                     IFMYHNIPDLGAIVYVMGFAIVCFLGGLAIFRKLEKGFAEEV"
     gene            complement(3030..4157)
                     /locus_tag="PLK68_04380"
     CDS             complement(3030..4157)
                     /locus_tag="PLK68_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89680.1"
                     /translation="MENIENAIEVRNMSKDFKLVFDKPATLKERLVFWNREKPQWHHV
                     LKDINLEIKKGDTIALVGTNGSGKSTLLKLMTKILYPTKGTLETAGKLTSLLELGAGF
                     HPDFTGRENIYFNASIFGLTKTEIEKRIDEIIEFSELGSFIDNPVRTYSSGMYMRLAF
                     SVAINVDAEILLIDEILAVGDQHFQDKCFAKLKELRDSDKTIVIVSHSLDMIRKLCTR
                     AVWIYKGELKADGSPNEVIDKYLDQVVKDHASQIGDYIAETISCILTVETPVEGEVKD
                     NSSNIIVSGWEVSNTMNTNVDIYLDNKYIGRAERHKRNDVLMHYQEANGGASMNAQAG
                     WEIKVPVEDIEGNHIIYVRVSNGSEMIADKQVQISLKGKSR"
     gene            complement(4264..>5022)
                     /locus_tag="PLK68_04385"
     CDS             complement(4264..>5022)
                     /locus_tag="PLK68_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89681.1"
                     /translation="IKKTVDFLRKEKFPEEFGLTETTILVRKNNSKKVDDVMGLWFKL
                     LMKYSIRDQLSFDYSVYRVGADIQRLDLNVFDNKWFGWVKHNKKIDFNVCRVYFDDYV
                     DIYESKLLDIPIKKEDGYSIYFKVTKKCSYIELHMGQLMGHRLKNLSIHSNKNIEIAT
                     NCCIAIDNYYIMGNDPLVIRLIGNFEINDKVEVNFNLSKILIDDWANIFSKLNKDFEG
                     ILMQKNYEIETLQAEYNSLKNRKIIKMLDKLKRK"
BASE COUNT         1669 a          961 c          494 g         1898 t
ORIGIN      
        1 tattatctac ccatcctcta attacaagag ttgggttttt tataccgcaa ataattgcct
       61 catcgaattt atatctcaag cgaatcacct tcttttctca cgatcaagca tataaactcc
      121 cattctacta aaattagaat tataatatgg atcattttca atataactag accatttctc
      181 atacataaaa ttattttcac tcacaaactg tttatatttt tcactagttg aatcaagtcc
      241 cctagtcttt gattcatagt ggattaactc aacttgagga ataaatagat tgtaatatcc
      301 tgcatctaat aatttaagat taaaatcaat atcattataa gctaccttta atttttcctc
      361 taatccatta acttgcaaat actttttccg ttctacagct aaacaagctg ctgtcacagc
      421 tgaataatta tatggtattt tcaatctacc atatacccct tcatcatctc ggggatggct
      481 tataaaagca tgtgctgcca cagcattacc taaaccaagt aagactccac catgttgaat
      541 tgtcatatcc ggatataata atttagagcc tacagctcca atgtgatctt gaatagcgta
      601 agatactagc aattttaacc aatttggtgt tattacttca gtatcgttgt ttaataatat
      661 aatgacatca gaaatacaag tatcaactgc taaattatta attgcagaat aattaaagtc
      721 catgtctgct ttaacaaccc taaaattggt atttgcttct tgatatctct taaacaaatc
      781 aaacgtttcc tgatttacac tattattgtc tactactact acctcataat tagtatactc
      841 tgtaagttca taaattgatt tcaagcaatt ttcagtaata tcggcaaagt ctcttgtagg
      901 aataattatt gaaacactag gttcttcttt ataattgtac tctactctat aaactgtact
      961 cccaccaata ctttgaacgt tagcagaaac atttcttctt tttagtgctt cttcaactgc
     1021 tcgaattcct ttatttactg catatccttt attatcaatt gccatagatg ttgatccttc
     1081 aaccattcgc caatgatata atattttagg aatatgataa atattttttg tttgctcact
     1141 cactcgtaaa aatagatcgt gatcctgtac accttctaat cctactgtga atcctcctaa
     1201 atatctcact aaactagtcc ttaatacggc aaaatggcaa atataattta ttcctagtaa
     1261 tgtatctggt gcaaaatcgg atttaaaatg tggttcacat cttgaccctc taatatctaa
     1321 cttgtcctca tcactatata taaaatctac attttcatat ttatttaata gtgctacatt
     1381 ttcatatagt gcattagctg ctaacatatc atcattatca actaaacaaa caaattcacc
     1441 tttagccata tctaaagcat catttgatgc cctagaaata tgtccatttc tttttcttct
     1501 attaacacgt atacgactat ctttattttc atattctttt atggtttcaa ttgtttcttc
     1561 attcgtagaa caatcatcga ccaaacatac ttcaaaattt tgatatgttt ggtttaaaat
     1621 tgattctaag cattctacta aatatttttt ttccacatta tatactggaa taagaataga
     1681 cattagtgga ttatactcaa atacttcaat atgttcatca ttttcaaaaa tattaatcca
     1741 tttatgataa tcatcttgaa taaacggatt ccacagtatt tctccacgct cttttacacg
     1801 cataaaaaat cttaggaaat attttttcca tagtgatggt ggaataatca tatgatgttc
     1861 tctccacaaa aatctcattc cccttccgaa tacaattata gatcttctta ttatagaaac
     1921 aagctttaac aaggttatct taaatttaaa cactaaagaa atatctagtg tatatatcct
     1981 ttctattgat tcatcattat tacaagtaat atatagttgt ttgcaatcct ttggaatcgc
     2041 gacttctatc tgaatttctc catcatctga tattattttt ttattgaaat caatttcttc
     2101 actatttaca tacacacgaa aaacatcatc ttctaatgaa ttagaaaaaa caactttaag
     2161 gtattgaact ttaattccag taatttcatc tttaacatat actatttttc tatctgattc
     2221 agtaaaattg gattcaccca tcttatacct cctctgcaaa gcctttttct aattttctaa
     2281 atattgccag cccaccaaga aaacaaacta ttgcaaatcc cataacatat acaattgcac
     2341 ccaaatcagg aatattatga tacataaata tgtcacgata aattaccatt aaatgcttaa
     2401 atgggtttaa attaataatt ttcattaata atgttggaaa tttatcaaat gtacttaaat
     2461 cgtacaaaat aggtgtacca taaaaaagca tatttacaaa gaactgaata atatactcag
     2521 tatctttaac atatacatta atcgcactta accccaaaac taatcccatg ataataaaat
     2581 attgcacaat cgcaaacact ggaaataaaa ttatatgcca tgataatcca actccaccaa
     2641 ataaacaaaa taatatcata atgatagatg atataaaaaa gtttactaat ccacttgttg
     2701 ctacagatat aggcagtatt tctctaggaa aatacacttt cttaataatt ccttcattga
     2761 ctcttattgt cataatccct tgattcattg ttgttgtaaa aaaagtccat gggataacgc
     2821 caattactaa gtatactaaa taattatcta cagtatccct aaataaaaaa ggaaatacaa
     2881 gaaaatagac cattaccata agaagtggat ttagaaatga ccaaagaacc cctaacatag
     2941 accccttgta cttacctcta atttcttttt taatatttga ttttagtagt tctctatatt
     3001 gatatagatt tctaattaac ttcataacat tacctactct taccctttaa acttatttgt
     3061 acttgcttat cagcaatcat ttctgacccg ttactcactc gtacatatat tatatgattg
     3121 ccctctatat cttccacagg cacctttatt tcccatccag cttgtgcatt catacttgca
     3181 ccaccatttg cttcttgata gtgcataagc acgtcatttc ttttatgacg ttcagcacgt
     3241 ccaatatatt tattgtctaa atatatatcc acattagtat tcattgtatt acttacttcc
     3301 cagcctgaaa caataatatt gctactatta tctttgactt caccctctac aggggtttcc
     3361 actgttaaaa tacagcttat tgtctctgct atataatctc caatttgact tgcatgatcc
     3421 tttaccactt gatctaaata cttatctatt acttcatttg gagagccatc tgcttttaac
     3481 tcgcctttat agatccaaac tgctctagta catagttttc tgatcatatc taaactatgg
     3541 gatactatga caattgtttt atcactatca cgtaattctt ttagcttagc aaaacattta
     3601 tcctgaaagt gttgatcacc cactgccaat atttcatcaa taagcaatat ttctgcatca
     3661 acattaattg ctaccgagaa agctaaacgc atatacatac ctgatgaata tgttcttact
     3721 gggttatcaa taaatgaacc taattctgaa aattcaatta tctcatcaat tcttttttct
     3781 atttcagttt tagttaatcc aaaaattgag gcattaaaat atatattttc tctaccagta
     3841 aaatcaggat gaaatcctgc tcccagttct aacaatgatg ttagttttcc agctgtttcc
     3901 agagtacctt tagtcggata caaaatttta gtcattaatt ttaaaagagt agattttcca
     3961 ctaccatttg ttcctactaa agcaatagta tctccttttt ttatctctaa atttatatct
     4021 tttaaaacat gatgccattg tggtttttct ctattccaaa aaaccaacct ttctttcaat
     4081 gtagcaggtt tgtcaaatac aagtttgaaa tctttactca tatttcttac ttcaattgca
     4141 ttttctatat tttccatgtt atccaccttt ataaatatat attagttcta ctataacaat
     4201 tcactgcatt ttactgtatt tttcagtttt ttgcaagttt ccttctatta tattggatat
     4261 attctatttt ctctttaact tatctaacat tttaattatt tttctatttt tcaagctatt
     4321 gtactcagct tgcaaagtct caatttcata attcttctgc attaatattc cttcaaaatc
     4381 tttattcaat ttactaaata tatttgccca atcgtcaatt aatatttttg acaaattaaa
     4441 attcacctct actttatcgt taatttcaaa gtttcctatt aacctaataa ctaacggatc
     4501 attacccata atataataat tatcaattgc aatacaacaa tttgtagcaa tttctatatt
     4561 tttattagaa tgtatactta aatttttaag cctatgtccc attaattgtc ccatatgcaa
     4621 ttcaatataa gaacattttt ttgttacttt aaaatatata ctgtacccat cctccttttt
     4681 tataggaata tctaataact tactttcata tatgtcaaca taatcatcaa aataaactct
     4741 gcaaacatta aaatcaattt ttttattatg ttttacccac ccaaaccatt tattatcgaa
     4801 aacatttaag tctaatcttt gtatatctgc acctactcta tacacactat aatcaaatga
     4861 taattggtcc ctgatactat atttcatcaa caatttaaac cataacccca ttacatcatc
     4921 tacttttttt gaattattct ttctaactaa aatagttgtt tctgtaagtc caaattcttc
     4981 tggaaatttt tcttttctta aaaaatcaac tgtctttttt at
//
LOCUS       DAOMTA010000113         5017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_384_length_5017_cov_2.452237, whole genome shotgun sequence.
ACCESSION   DAOMTA010000113 DAOMTA010000000
VERSION     DAOMTA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_5017_cov_2.452237"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..616
                     /locus_tag="PLK68_04390"
     CDS             44..616
                     /locus_tag="PLK68_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HRM89682.1"
                     /translation="MTKKIIVETSARHIHLSDADLETLFGKGYQLTNKKNLSQPGQFA
                     CEEKVTVVGAKGEQKMSVLGPTRKATQVELSLTDARALGLQAAIRESGDIEGTAGCKL
                     VGPAGEVEISCGVIAAKRHIHMTPADAVEFGVTDKQIVKVEIETEGRSLIFGDVVCRV
                     SENYATAMHIDTDESNAAGCGREVYGTIIK"
     gene            687..>1222
                     /locus_tag="PLK68_04395"
     CDS             687..>1222
                     /locus_tag="PLK68_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRM89683.1"
                     /translation="MKILDTLGKTKKAIGGIIAILLVALLIFYAGTRFASSSEPKISS
                     TGLSQQLQEIEELATMSYNYTKVGKFSNNLTFNGWDIPLTQKSFLITYDGKLKAGVKM
                     DKIEVAINNNIITVSIPEIEILSNEIDESSIEVYDETKNVFNPISVNDYTTFAKKQKE
                     AVAEEAIENGLLSEAATKT"
     assembly_gap    1223..1232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1349..>2821)
                     /locus_tag="PLK68_04400"
     CDS             complement(1349..>2821)
                     /locus_tag="PLK68_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM89684.1"
                     /translation="NECYDENGVNLWQWMSVQTKADKVIASGSQKAYIIGDDWGPAVT
                     KTVISLDKAIDADSVVAENFKVVEEKEATINWGTGEIGIATADRNVTAAYTSDAQGNK
                     VTGSSKYITIEMYVSPSEGSPFIYNLKTGFNSWCTPYKLNVSLAKGATMSSGDEVVTD
                     LNIKADIDVAGDGKICPQGEVFEMKAYTAKDGTTYSYADYTPAKDDKKNALVIWLHGA
                     GEGGTDPYIDILGNEVTSLVSKEFQSLFEGAYVLAPQSPTMWMDDGTGAYQNGDKGSM
                     YAESLFEMIDAYVKANDDIDPNRVIIGGCSNGGYMTMEMVLKHPTYFAAAFPICEAFQ
                     DQYITDDQINAIKDMPIWFTYAKNDGTVDPTLCVEPTVARLLAAGANNIHVSVFDDVH
                     DTTGRFFNEDGTPYQYNGHWSWIYFDNNECYDENGVNAWQWLAKQIKTAAPVETPDQP
                     TTPDQPANSVKTGDDVNFAGLGAIMMLTLAGIYVSRRKYN"
     assembly_gap    2822..2864
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2865..4050)
                     /locus_tag="PLK68_04405"
     CDS             complement(<2865..4050)
                     /locus_tag="PLK68_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM89685.1"
                     /translation="MFKKILSCLMSAMMVLGLTILPTHALEGTATGTQKAFVVGDDWG
                     AGVTKSIITFDKKIKADSISKTDFLVKETATNKVSDRTILDAYASDAQGNKVTTDSNI
                     VTVEMYISPSEGNPISWNNLTWKNAWADPYQLEVTLAGGETLVSENETITTIDVDPTI
                     NVAEDGKICPQLDGFKMSEFTNDGTTVSYALYTPENDSHKNALVIWNHGVGETGTDVQ
                     IDLLGNEVTALAGDEFQNTMDGAYVLVPQRRSYDSTSNAEAIYQLILKTLRENPDIDQ
                     DRIIIGGCSAGGAMTMTMIIAHPELYAAAYPICPATQSANVSDETIESLKDLPIWFTH
                     AKNDSTVAIATTTEPLVERLRAAGAEVHTSIFDDVHDTTGRFSNEDGTPYQYDGHWSW
                     TYF"
     gene            complement(4130..4963)
                     /locus_tag="PLK68_04410"
     CDS             complement(4130..4963)
                     /locus_tag="PLK68_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89686.1"
                     /translation="MYKYEKVEHDNELPTKLHDFYIEDNIGEPIEKHWHRSIEILIPL
                     YGSFILWINGNKVKITAGNIYIINSQDIHAIQAIEGERIYKGYALQIKYDYLRESYHD
                     IDKIYFQQPNQQINKLLMAKIIDIINFYESDDPYNNIRVKSHIQMLVFLLLDNLSKKR
                     IGYLEVKDSKYKDRITKIIKYLENNYQEDLSVKMIADEFGLSEGYLSKLFKESLGATV
                     KEYLSRVRLWHAEEQLVETDYPVIDIAIGNGFPNVKSFNQAFKRKNVITPAKYREKMR
                     K"
BASE COUNT         1626 a          901 c          732 g         1705 t
ORIGIN      
        1 cgataaaagg agtaatatag aataaggaaa aaggagaatg actatgacta aaaaaattat
       61 cgttgaaact tcagcaagac acattcattt atctgacgct gacttagaaa cattatttgg
      121 aaaagggtat caattaacta ataagaaaaa tctttctcaa cctggacaat ttgcatgtga
      181 agaaaaagtt actgtagttg gagctaaggg tgaacaaaaa atgtctgttt taggaccaac
      241 tcgtaaagct actcaagttg agttatcatt aactgatgct cgtgctttgg gattacaggc
      301 tgctatcaga gaatctggag atatcgaagg aactgcagga tgtaaactag taggaccagc
      361 tggtgaagtt gaaatttctt gtggcgttat cgctgctaaa agacatatcc atatgactcc
      421 tgctgatgcg gttgaatttg gagttactga taaacaaatc gttaaagttg aaatcgaaac
      481 tgaaggacgt tcattaattt tcggtgatgt tgtttgtcgt gttagtgaaa attatgctac
      541 agctatgcat attgacacag atgaatcaaa tgcggctgga tgtggtcgtg aagtatacgg
      601 aacaatcatt aaataataaa gcctttgggc ttttttattt tctcaataat tgtcgaatta
      661 tgttataatg tccctaggtg ataaaaatga aaatattaga cacattagga aaaacaaaaa
      721 aagctattgg tggaatcatt gcaatcctac tagttgcatt actgattttt tacgcgggaa
      781 ctcgatttgc aagcagcagt gaaccaaaga tatcgtcaac tggtctgagt caacagctcc
      841 aagaaatcga agaattggca acaatgtcct ataattatac taaagttggt aaattttcta
      901 ataatttaac atttaatggc tgggatattc ctttaactca aaaaagtttt ttaattacat
      961 atgacggtaa acttaaagct ggtgtcaaaa tggataaaat tgaagtagct attaataata
     1021 atataattac cgtttcaata ccagaaattg aaattttaag taatgaaatt gacgagagta
     1081 gtattgaagt atatgatgaa acaaaaaatg tattcaaccc aatttctgtc aatgattata
     1141 ctacctttgc taaaaaacaa aaagaagcag tagctgaaga agcaatcgaa aatggtttat
     1201 taagcgaagc agcaactaaa acnnnnnnnn nngtacaatt aaaaaatatt tacatgcaat
     1261 tccgggaatt gatggcaact atgagatcaa agttaaattt ttagaaacaa aaaaagagag
     1321 ctagaaattc aaatctagct cttttaattt aattatattt acgtcgagat acataaattc
     1381 ctgcaagagt tagcatcata atagctccta atcctgcaaa attaacatca tcaccagttt
     1441 tcactgaatt cgctggttga tctggagttg ttggttgatc tggggtttct actggagcag
     1501 cagttttaat ttgtttagct aaccattgcc acgcatttac accattttca tcataacatt
     1561 cattattatc aaaatagatc catgaccagt gaccattata ttgatatggt gtaccatctt
     1621 cattaaagaa tcttccagtt gtatcatgca catcatcaaa tactgataca tgaatattat
     1681 tagccccagc agctaataat cttgctactg ttggttctac acataaagtc ggatcaactg
     1741 taccatcatt tttagcatat gtgaaccaaa ttggcatatc cttaattgca ttaatttgat
     1801 catctgtaat atattgatct tggaatgctt cgcagattgg gaaagccgct gcaaaatatg
     1861 ttggatgttt taaaaccatt tccatagtca tatacccacc attagaacat cctccaataa
     1921 taacacgatt tgggtcaata tcgtcattag cttttacata agcatcaatc atttcaaata
     1981 aactctcagc atacattgaa cctttatcac cattttgata agcacccgtt ccatcatcca
     2041 tccacatagt tggactttgt ggtgctagta cataggcacc ttcaaataag ctttggaatt
     2101 ctttagatac taaagatgtt acttcatttc ctaaaatatc aatgtatgga tccgttccac
     2161 cttcacctgc accatgtaac cagataacaa gagcattttt cttatcatct ttggctggtg
     2221 tataatcagc atatgaatac gttgtaccat ctttagcagt ataagccttc atttcaaaaa
     2281 cttcaccttg tggacaaatc tttccatctc ctgcaacgtc aatatctgct ttaatattta
     2341 aatcggttac tacttcatca cctgaactca ttgtagcacc ttttgctaaa cttacattta
     2401 atttataagg tgtacaccat gagttaaatc ctgtttttaa attataaata aatggtgatc
     2461 cttcacttgg agaaacatac atttcaattg taatatattt agatgaaccg gttactttat
     2521 ttccttgggc atcagaagta taagctgcag ttacattacg atctgcagtt gcaataccaa
     2581 tttcaccagt tccccaatta attgttgctt ctttttcttc aacaacttta aagttttcag
     2641 caactacaga atcagcatca attgctttat ctaaactaat tactgttttt gttactgcag
     2701 gcccccaatc atcaccaata atataggcct tttgagaacc acttgcaatt actttatcag
     2761 ctttagtttg tactgacatc cattgccata agttaacacc attttcatca taacattcat
     2821 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaaaat aagtccatga
     2881 ccaatgtcca tcatattgat atggtgttcc atcttcatta gagaatcttc cagttgtatc
     2941 atgcacatca tcaaaaattg atgtatgtac ttcagcacct gctgcccgta atctttctac
     3001 aagaggttct gttgtagtag cgatggcaac agttgaatca ttttttgcat gagtaaacca
     3061 aattggtaaa tctttaagtg attcaattgt ttcatcagac acattcgcac tttgagtagc
     3121 aggacaaatt ggatatgctg ccgcatataa ttctggatgc gcaataatca tggtcattgt
     3181 cattgctcca cctgcagaac atccacctat aataattcta tcttgatcaa tatcaggatt
     3241 ttctctaagt gtttttaaaa tcaactgata aatagcttca gcattagatg tactatcata
     3301 tgatcttctt tgtggcacta aaacatatgc tccatccatt gtattttgga attcatcacc
     3361 agccaaagcc gtaacttcat ttcctaataa atcgatttga acatctgttc cagtttcacc
     3421 aacaccatga ttccaaataa ctaaagcatt tttatggcta tcattttctg gtgtatataa
     3481 agcatatgaa acagtagtac catcattggt aaattcactc attttaaaac catctaattg
     3541 aggacaaatt ttaccatctt cagctacgtt aatagttgga tctacatcaa ttgttgtaat
     3601 tgtttcattt tcagaaacta gagtttcacc acctgccaaa gtaacttcta actgatatgg
     3661 gtcagcccat gcatttttcc aagttagatt attccaagaa attggattac cttcacttgg
     3721 agaaatatac atttctactg taacaatatt tgaatcagta gttactttat ttccctgagc
     3781 atcagaagca taagcatcta aaattgttct atcacttact ttgtttgtgg cagtttcttt
     3841 aactaaaaaa tctgttttac tgatagaatc tgctttgatt tttttgtcaa aagtgataat
     3901 ggatttagtg acaccagcac cccaatcatc acctacgaca aaagcttttt gagttcctgt
     3961 tgctgttccc tctagggcat gggttggcag tattgttaaa cccagtacca tcatagcact
     4021 cattaagcaa cttaaaattt tcttaaacat cctttctcct cctttttcaa tatatatatt
     4081 atataatttt aagcgcttac attaatggcg ttttattatc taatgaatgt cattttctca
     4141 tcttttcacg atactttgcg ggagttatca cattttttct tttaaaagct tgattgaatg
     4201 atttcacgtt aggaaagcca ttgccaatag caatatcaat aaccggatag tctgtttcca
     4261 ctaattgttc ttccgcatgc cataatcgaa cacgactcaa atattctttc actgttgccc
     4321 ctaagctttc tttaaataac ttagatagat atccttctga taatccgaat tcatcagcaa
     4381 tcattttaac tgagagatct tcttgataat tattttctaa atacttaata atctttgtaa
     4441 ttcgatcttt atatttacta tctttaacct ctagataccc gattcttttt ttactaagat
     4501 tatctaataa taagaaaaca agcatctgaa tatgactttt aactcgaatg ttattatacg
     4561 gatcatcact ttcataaaaa ttaataatat caattatttt agccataagt aatttattga
     4621 tttgttgatt aggctgttga aaataaattt tatctatatc atgatacgat tccctcaaat
     4681 aatcatattt tatttgtagg gcgtagccct tatatatacg ctcaccctcg atagcctgaa
     4741 ttgcatgaat atcttgagaa ttaataatat aaatatttcc agctgttatt ttaactttat
     4801 taccatttat ccataaaata aatgaaccat ataatggtat cagaatttca atacttcgat
     4861 gccaatgctt ttcaatcggt tctccaatat tatcttcgat ataaaaatca tgtaatttag
     4921 tcggcagctc attatcatgt tcaacctttt catatttata catattagta ccttcctcca
     4981 atattaccat ttaataatat cacatattta actttat
//
LOCUS       DAOMTA010000114         4998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_388_length_4998_cov_1.974509, whole genome shotgun sequence.
ACCESSION   DAOMTA010000114 DAOMTA010000000
VERSION     DAOMTA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_4998_cov_1.974509"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            531..2798
                     /locus_tag="PLK68_04415"
     CDS             531..2798
                     /locus_tag="PLK68_04415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM89687.1"
                     /translation="MMNNKNTAKKMSINENAILEKYLNTLATPISKHKPDKKLSFIWA
                     NDAFYKMTGHEKKDFKMIWQDLKTYYKPYTDDFIILENQLAKLQEHRDLHQECIIRIP
                     FRHDEIKWIKLSISLSFEVITIAYNDISNLINRQNTLISTKNVYIDNFQWMMAENAGN
                     VYISDIDTYELLYLNKVACETLGTRAEQAIGKKCYEIIQGRSSPCPFCTNKYLKKDQT
                     YEWEFYNPLLKRNFIIKDRMLNWHGHSARIELSHDTYSTEYKLAKKDQEREAILKTIS
                     AAMVRVDARDYSSILWCNDIFLKMIGYTKEQFKSERYELKSYMHPEDLKRAKQIVHKL
                     RNSKESVVFEGRVYTRNKEERIWTLTLCYVDERDSWDGIPCFYSMGLDITDERQKIKT
                     LQHKAEKDALTGIYNRSETENQIKNFIIEHHDSKGALFMIDTDNFKQINDTLGHIAGD
                     MVLGEMATAMKKKVRDSDVVGRIGGDEFIIFMKNITSHLDAEKKAEELQKTFAHLFDK
                     EKSKIQVTSSIGIALYPKDGHNFLELYANADKALYQAKIQDKNTYIVYNEKSCNQQSI
                     TAYSSLGATIDSQQSYSETSVSLADYAFRILFQNKDLKTAINLILEITGKRYDVSRAY
                     IFETMTDKEHCSNTYEWCNEGITAVKEQLQNISYHDMDEYERVFDENSIFYCHDVTSL
                     SKKQRQLFEAQGIHSTLQCAYYEQEMIAGFVGFDECTGKRIWTKEELRMLTLISQIIS
                     IFLKHKGLSDQVSKL"
     gene            3241..3618
                     /locus_tag="PLK68_04420"
     CDS             3241..3618
                     /locus_tag="PLK68_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HRM89688.1"
                     /translation="MSQKIKRGDIIDVKITGIQPYGAFASLPDNSTGLIHISEISDKF
                     VKSIDSFVKVGESIKVKVIDFDQETNHAKLSLKAIDNRYRRRNKRVYYKNPRRLIVET
                     PNGFKPLALAMEKWLKQGITEES"
     gene            3620..4900
                     /locus_tag="PLK68_04425"
     CDS             3620..4900
                     /locus_tag="PLK68_04425"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRM89689.1"
                     /translation="MITLDLSKAKLSEDLESYKDKVKEIHDMIHNKTGAGNDFLGWVE
                     LPENYDKAEVELIKETAASLREKTDVLLVCGIGGSYLGARAAIEAINGLYPTNDVEII
                     YVGNTFSSNYIKQVADYIKDKDFAINVISKSGTTTETSIAFRIFKEMCETKYGKVGAK
                     ERIVATTDKAKGALKTLATEEGYTTFVIPDDVGGRYSVLTPVGLFPIAMAGIDVDAML
                     KGAKDAMEKYGNADLDQNDAYKYGVARQILHKAGYPAEMFVTYELQLAMVAEWWKQLY
                     GESEGKEGKGILPTSATFSTDLHSLGQFIQEGTKVLYETIMQIKEPAGDITIPSDKDD
                     LDGLNYLAGKSVDYVNKKACEGTVDAHVNTGNVPNILITLDKMDAYGFGYMVYFFEMS
                     CAMSVYLLGVNPFNQPGVEVYKANMFKLLGKPGY"
BASE COUNT         1857 a          717 c          820 g         1604 t
ORIGIN      
        1 tgtaacacag ataaagagcc ccactaaact gaggttcttt atctgtctga caaatgctct
       61 aactacgtac atatatgcta aaattacaaa gctagacaca atattttaat ttaaatataa
      121 ggattgattt ctcactcata atgaagtcaa tctttttaat tatgttttga ttttttgttc
      181 ttaataacaa aaatatcctt ctatttccta ctttgttcta atgattcata ttcataaaat
      241 atttcattct tcataaaaaa tcctctatga cttaattacc aaaacaaaac tttactataa
      301 taccttcgct tctatcaaat tttagtctct ctattaaatc attatactat ctgaccacta
      361 ccaaaattaa caaaaattta ttgtaaaata ttttttatta gatatttcaa tattacattt
      421 taacttcatt aactattcag cagaacgaaa agatagcgtt ttcaaattac tatgttataa
      481 taaaagaatg atatgtgtga tataaatgtg atatatcagg aggggtggtt atgatgaaca
      541 ataaaaatac tgcaaagaaa atgtcgatta atgaaaatgc tattttagaa aaatatttaa
      601 atactctggc aactccaatt agtaaacaca aacctgataa aaaactctca tttatatggg
      661 caaatgatgc tttttataaa atgaccgggc atgaaaaaaa agattttaaa atgatttggc
      721 aggatttaaa aacatactac aaaccttata cagatgattt tattattctt gaaaatcaat
      781 tagcaaagtt gcaagagcat cgtgatttac atcaagaatg tataatccga attcctttta
      841 gacatgatga aataaaatgg attaaattat ctatttcact ttctttcgaa gtaataacaa
      901 ttgcatataa cgatattagt aatttaatca atagacagaa tacactcatt tcaactaaaa
      961 atgtatatat agataacttt caatggatga tggcagaaaa tgccggaaat gtttatatca
     1021 gtgatattga cacatatgaa cttctatatt taaataaagt tgcatgtgaa acgcttggta
     1081 ctagagctga acaagcaata gggaaaaagt gttatgaaat catacaaggt agaagttccc
     1141 cctgtccttt ctgtacaaat aaatatttaa aaaaggatca aacttatgaa tgggagtttt
     1201 ataacccttt attaaaaaga aattttatta tcaaggatcg tatgttgaat tggcatggcc
     1261 actctgcccg tattgagctt tcccatgaca cttatagtac tgaatataag ctagcaaaga
     1321 aagatcaaga acgagaagca attttaaaaa ccatttctgc cgcgatggta cgtgttgatg
     1381 caagggatta ttcttcaatt ctctggtgca atgatatttt tcttaaaatg attggttata
     1441 caaaagaaca atttaaaagt gaacgttatg agttaaaaag ctatatgcat cctgaggact
     1501 taaaaagagc caagcagatt gttcataaat taagaaattc aaaagagagc gttgtattcg
     1561 agggacgagt ctatacccgt aataaagaag aacgtatctg gacactgaca ttatgctacg
     1621 tagatgaaag ggacagctgg gatggtatcc cttgttttta tagtatgggt ctagatatta
     1681 cagatgaaag acaaaaaata aagacattac agcataaagc agaaaaagat gcattaactg
     1741 ggatatacaa ccgctctgaa accgaaaatc aaataaaaaa ctttattatt gaacatcatg
     1801 actctaaggg agcactcttc atgattgaca cagataattt taaacaaatc aatgatacat
     1861 tgggacatat tgctggagat atggtattag gtgaaatggc aacggctatg aaaaagaaag
     1921 tacgtgatag tgatgtcgtt ggtagaattg gtggtgacga gtttattatt ttcatgaaaa
     1981 atattactag ccacttagat gcagaaaaga aagctgaaga actacaaaaa acatttgccc
     2041 atctatttga caaagagaaa agtaagattc aggtaacaag cagtattggt atcgctttat
     2101 atccaaaaga tggacataat tttttggaat tgtatgcaaa tgctgataaa gccctttacc
     2161 aagccaaaat ccaagacaag aatacatata tcgtatataa tgaaaaatct tgtaatcagc
     2221 aaagtattac cgcatattct tcactaggtg caactatcga ttcacaacaa tcttatagtg
     2281 aaacatctgt ttcattagca gattacgcct ttcgtattct atttcaaaac aaagatttaa
     2341 aaacagctat taatctgatt cttgaaatta ctggaaaacg ctatgacgta agccgtgctt
     2401 atattttcga aaccatgaca gacaaagaac attgctccaa tacatatgaa tggtgtaatg
     2461 aagggatcac cgctgtaaaa gaacaactac aaaatatttc ttatcatgat atggatgaat
     2521 atgaaagagt attcgatgaa aattctatct tctactgtca tgatgtcacg tcattaagca
     2581 aaaaacaaag acaactgttt gaagcccaag gaattcattc tacactacag tgtgcgtatt
     2641 atgagcagga aatgattgcc ggtttcgttg gctttgatga atgtacagga aaacgaatat
     2701 ggacaaaaga agagcttaga atgcttactc tcatatcgca aattatatca atctttctaa
     2761 agcataaagg attaagtgac caagtatcaa aattgtaatc tagttattgt tttagactac
     2821 taataaataa aatatatcag taatttaaag atatttagaa aataatctta tatacacaaa
     2881 taggctggac aataattcca gcctatttat attcattaat tctctaatcg tcttttaaaa
     2941 ctagtaaagc catcttactt ctaccagcaa atcattcatt tccttttcac ataattttag
     3001 aataactatt cttctactac attctttaaa ttgttattcc tggttctaga tggcgtaata
     3061 ctttattctt agcttaaatt taattttaac aaccttaaaa atgtattctt ttacttaata
     3121 aaaaaatata ctaaatttat tggatctatt gccttatcaa tgatttttgt attatttata
     3181 aaaaatgaaa taattttatt tattttgtgc tataatatcc acgcagggag tgattattaa
     3241 atgagccaaa agattaaacg cggtgatatc attgatgtca aaattactgg tatccagcca
     3301 tatggagctt ttgccagttt accagataat tcaacaggat taatacatat ttccgaaata
     3361 tctgataaat tcgttaaaag catcgatagt tttgttaagg tgggtgaatc aatcaaagta
     3421 aaagttattg attttgatca ggaaactaac catgctaaac ttagcttgaa agccattgat
     3481 aatcgttatc gtagacgcaa taaacgtgtt tactataaga atcctagacg attaatcgtt
     3541 gaaacgccta atggtttcaa accattagct cttgccatgg aaaaatggct taaacaagga
     3601 atcacggagg aaagttaaaa tgattacatt agatttatct aaagctaaat taagcgagga
     3661 tttagaatca tacaaggaca aagtaaaaga aatccatgat atgattcaca acaaaactgg
     3721 agctggtaat gattttcttg ggtgggttga attacctgaa aattatgata aagctgaagt
     3781 tgaattaatt aaagaaacag ctgctagtct aagagaaaaa acagatgtgt tactagtttg
     3841 tggtattggg ggatcttact taggtgcacg tgcagcaatt gaagcaatca acggattata
     3901 tccaactaat gatgttgaaa tcatttatgt aggaaatact ttttcatcaa attatattaa
     3961 acaagtagct gattatatta aagataagga ttttgctatc aatgtaattt ctaaatcagg
     4021 tacaactaca gaaacttcta tcgcatttag aattttcaaa gaaatgtgcg aaactaaata
     4081 cggtaaagtt ggagctaaag aaagaatcgt tgctacaaca gataaggcta aaggtgcatt
     4141 aaaaactttg gctactgaag agggatatac gacttttgta attcctgatg atgttggtgg
     4201 ccgttattca gttttaacac cagtaggatt attcccaatc gcaatggctg gaattgatgt
     4261 agatgcaatg ttaaaaggtg ctaaagatgc gatggaaaaa tacggtaatg ctgatcttga
     4321 tcaaaatgat gcttataaat atggtgttgc tcgtcagatt ttacacaaag ccggataccc
     4381 tgctgaaatg tttgtaactt acgaattaca actagctatg gtagctgaat ggtggaaaca
     4441 actttacggt gaatcagaag gtaaagaagg taaaggtatc ttacctacta gtgctacttt
     4501 ctcaactgat ttacattctt taggacaatt tatccaagaa ggtacaaaag ttctttatga
     4561 aacaatcatg caaattaaag aaccagctgg agatatcact attcctagtg ataaagatga
     4621 tttagatggt cttaattatt tagcaggtaa atctgtagac tatgtaaata aaaaagcttg
     4681 tgaaggaact gttgatgctc atgttaatac tggtaatgta cctaacattt tgattacttt
     4741 agataaaatg gatgcttatg gttttggtta catggtatat ttctttgaaa tgtcatgtgc
     4801 tatgtctgta tatttacttg gtgttaatcc atttaatcaa ccaggtgttg aagtgtacaa
     4861 agcaaatatg tttaaattac ttggtaaacc aggctattaa ttattatgac gatctttgat
     4921 cgtcttttca tttgcataaa tatttaaaat atggccaaaa tgtaaaagaa gtgcttatta
     4981 tattaaacta attataaa
//
LOCUS       DAOMTA010000115         4991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_390_length_4991_cov_2.407212, whole genome shotgun sequence.
ACCESSION   DAOMTA010000115 DAOMTA010000000
VERSION     DAOMTA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_390_length_4991_cov_2.407212"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..983)
                     /locus_tag="PLK68_04430"
     CDS             complement(3..983)
                     /locus_tag="PLK68_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRM89690.1"
                     /translation="MKTLITTLNSKYIHKSLSLRLLYVATKEYHEVDFKEYTIKEDLD
                     TIANDIIEQKVQVVAFSVYIWNVDLIKILIDKLKKMDPQLVIVIGGPEVSYEIDYFLD
                     NFKIDYLISGEGEVAFKELLDCLETQQPINIQGISRKDKRDYQQTKPVDLQYLETLES
                     PYLLKRDLADMDKRILYFETSRGCPYQCQYCLSSLEKGLRFFSLDYLKAQLSLLLDSG
                     VKTIKLLDRSFNAKTAHALAILEFIFKHYRPGIQFQFEINGDVLDQRIIDYINDYAPR
                     GLLRFEIGIQSTYEPTNEIVKRYQNFERLSEVIKQLQQNHKIDFHLDLIS"
     gene            complement(1024..2271)
                     /locus_tag="PLK68_04435"
     CDS             complement(1024..2271)
                     /locus_tag="PLK68_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM89691.1"
                     /translation="MFKKKRDYGYYSSYGTSSRSRLKVGRVALVGAVGVVLVIGIVVV
                     LNLTRIKLLIKGYSFSQASEILSLTKEQENEILSHDKLDHITNWIANSKKVSLYDEYE
                     KYLGMNKKMEYADVVTYINGLFKEEVPKLKSMGYSEKVIWNLISDKVSKEDLQYLIDH
                     NYTAKDLEPYRKVKGYQIQNLEAYINAYNNYKDYNYAVCITNYPFLISSNGEPKESYT
                     ITNPDDLLVLVKKGFYLPESYEPKLVDPEIPVAPDCQNPKMTKETSDALTKMYEAAKK
                     EGLELVVNSAYRSYQTQVDTMADFERRFGGQYANEYVAQPGASEHQTGLGIDLTSQSV
                     VEGKKITFGDTDEYKWVIKNCAKYGFIIRFENGTDGITGIAHEPWHLRYVGKDVAKKI
                     VEKEWTFEEYCLYNNVMPKVTKK"
     gene            complement(2301..2855)
                     /locus_tag="PLK68_04440"
     CDS             complement(2301..2855)
                     /locus_tag="PLK68_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRM89692.1"
                     /translation="MIKLTTPLTVEKIKQLHAGDEVLLSGTIYTGRDAAHKRLMALIE
                     EGKELPFHLEDQVIFYVGPTPSKPGKVFGSGGPTTSGRMDAFAPTMIKLGLRSMIGKG
                     YRQQAVKDAIIKYHGVYFGAIGGAGAMMSNCIKECTVIAFDDLGPEAIRRLEVEDMPL
                     VVVIDSNGNDQYELGRNDYLSTCK"
     gene            complement(2852..3694)
                     /locus_tag="PLK68_04445"
     CDS             complement(2852..3694)
                     /locus_tag="PLK68_04445"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRM89693.1"
                     /translation="MREIKCEDITKTVKQLCMEAACNLPSDVFKALKDKTDTEPYPLA
                     KKTLEVLIDNADLAKDNMMPICQDTGMAFVYITIGQEVHVDGDLKAAINEGVRQGYEE
                     GYLRKSVVDDPLFERINTKDNTPAIIYFDLVCGDEFKIVVAPKGFGSENMSQIKMLKP
                     SDGLQGVKDFVMKVVNDAGPNACPPMVIGVGIGGSFDKVTMLSKQAMMREIGTHHEDS
                     RYAALETELLEMINATGIGPAGYGGKTTALSLNIETHPTHIAGMPVAVSICCHVARHK
                     EASL"
     gene            complement(3731..4546)
                     /locus_tag="PLK68_04450"
     CDS             complement(3731..4546)
                     /locus_tag="PLK68_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89694.1"
                     /translation="MKSLFYRLIIFLDTASEADTNYNIAWYMAHNFSKVAKMGISQLA
                     KECYVSPATISRFCRALGYENYAHLKQECSSFASDSKKFNNLINVPLDMMKDKPQECT
                     KYYSDQVCDAISKLSQRLDWNVIDKVLQAIHDSDSVAFFGTQFSHSAALHFQTDLLML
                     EKFTLAYMETERQIDCARLMDENSVAIIVTVNGFYARSNSKVLQYLKKSKCKVVLMTN
                     NPGIDIGINVNYTIVLGDSEQRKIGKHTLLTAVELMSLRYYSLYYPGIQEKII"
     gene            complement(4844..4984)
                     /locus_tag="PLK68_04455"
     CDS             complement(4844..4984)
                     /locus_tag="PLK68_04455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRM89695.1"
                     /translation="MDQNLELADASMMPQLAAALQEAGFSENEIEKVFYKNVLRVYSQ
                     IL"
BASE COUNT         1612 a         1016 c          606 g         1757 t
ORIGIN      
        1 ccttacgata tcaaatctaa atgaaaatca attttatgat tttgctgtaa ctgtttaatt
       61 acctcactta accgttcaaa attttgatat cttttaacga tttcatttgt cggctcatat
      121 gttgactgaa ttcctatttc aaatcgtaat aatcctcttg gagcataatc attaatataa
      181 tcaataattc gctgatctag tacatcacca ttaatttcga attgaaattg aattcctggt
      241 cgataatgct taaaaataaa ttctaaaatc gccaaagcat gagctgtttt agcattaaag
      301 ctccgatcta gcagcttaat tgtcttaact ccactatcaa gcaataatga taattgcgct
      361 tttaaataat ctaaactaaa gaagcgtaat cctttttcta atgaagataa acagtactgg
      421 cattgatatg gacagccgcg acttgtttca aaataaagaa tccgcttatc catatcagca
      481 agatcacgtt ttaataaata aggtgattca agtgtttcaa gatattgtag atcaaccggc
      541 ttagtttgtt gataatctcg cttatctttt ctgctaattc cttgaatatt aatcggctgc
      601 tgtgtttcta aacaatctaa cagttcttta aaagccacct caccttcacc actaattaaa
      661 tagtcaattt taaagttatc tagaaaataa tcaatttcat aacttacttc cggtcctcca
      721 attacaataa ctaattgtgg gtccattttt tttaatttat ctatcaatat ttttattaaa
      781 tcaacattcc aaatatagac actaaaagcc acaacctgta ctttttgttc aataatatca
      841 tttgcaattg tatcaagatc ctctttgatc gtatattctt taaaatccac ctcatgatat
      901 tctttagtag ctacataaag caatcttaac gataaagact tatgtatata ttttgaattt
      961 aaagtagtaa ttaatgtttt catttcttta cctcattaaa ataatctaga ggtaacccct
     1021 agattatttt ttcgtaactt ttggcattac attgttatat aaacaatatt cttcaaaagt
     1081 ccattccttt tcaacgattt tttttgctac atctttacca acataacgta aatgccaagg
     1141 ctcatgagca attcccgtta ttccgtcagt accattttca aaacggataa taaagccata
     1201 tttagcacaa tttttaatta cccatttata ttcatcagta tcaccaaaag tgattttttt
     1261 accttctaca acactttgac tagttaaatc aattcctaaa ccagtttgat gttcacttgc
     1321 tccaggctga gcgacatatt catttgcata ctgcccgcca aaacgtcttt caaaatcagc
     1381 cattgtatca acttgtgtct gatatgaacg atacgcacta ttaacaacta attctaatcc
     1441 ttctttttta gctgcttcat acattttagt taaagcatct gatgtttctt tagtcatttt
     1501 cggattttga caatctggag ctactggaat ttccggatct actaatttag gttcatagct
     1561 ttctggtaaa tagaaacctt tttttactaa aactaataaa tcatcaggat tagtaattgt
     1621 atagctttct ttaggctcac cattagatga aatcaaaaat ggatagttag taatacatac
     1681 tgcataatta tagtctttgt aattattata agcgttgata tacgcttcaa gattttgaat
     1741 ttgataacct tttactttgc gataaggctc taaatccttg gcagtataat tatgatcgat
     1801 caaatattgt aaatcttctt ttgatacttt atcactaatc aaattccaaa taactttctc
     1861 actatacccc atacttttta gtttaggtac ttcttcttta aataatccat taatatatgt
     1921 aacaacatca gcatattcca tctttttatt catgcctaga tatttttcgt attcatcata
     1981 taaagaaact tttttagaat ttgctatcca attagtaata tgatcaagtt tatcatggga
     2041 taaaatttca ttttcttgtt cttttgttaa tgacaaaatt tcacttgctt gtgaaaatga
     2101 atagcccttg attaataatt taatccgtgt caaatttaac acaacaacaa ttccaataac
     2161 taagacaacc ccaacagccc caacaagagc aacgcgtcca accttaaggc gacttcgact
     2221 acttgttcca taactgctgt aatatccata atctcttttc tttttaaaca ttattatatt
     2281 acctctttaa tactttctct ttatttacat gtacttaaat aatcgttacg ccctaattca
     2341 tattgatcgt taccattact atcaatcact actactaatg gcatatcctc aacttctaaa
     2401 cgtcgaatcg cttcaggtcc aagatcatca aaagcaatta ctgtacattc tttaatgcaa
     2461 ttagacatca tcgctccggc tccaccgata gcaccaaaat acactccatg atatttaatg
     2521 attgcatctt taactgcctg ctgacgatat cccttaccaa tcatcgaacg taatcctaac
     2581 ttgatcattg ttggtgcaaa agcatccatt ctcccagacg ttgtaggtcc tccactccca
     2641 aatactttac caggtttact tggcgttggt cccacataaa aaattacttg atcctctaaa
     2701 tgaaaaggta attcctttcc ttcttcaatc aaagccatca aacgtttatg agccgcatca
     2761 cgacccgtat agatagttcc gcttagtaac acttcatcac cagcatgtaa ttgttttatt
     2821 ttttcaacgg taagcggtgt tgtcaattta atcataatga agcctcctta tgacgcgcta
     2881 catggcagca aatacttact gcaacaggca ttccagcaat atgagtagga tgagtctcaa
     2941 tattcaatga tagagccgta gtttttccac cataaccagc tggtccaatt cctgtagcat
     3001 tgatcatctc taataattca gtttctaaag cagcatatcg gctatcttcg tgatgggttc
     3061 caatttcacg catcattgct tgttttgaca acatcgtaac tttgtcaaat gaccctccga
     3121 tccctacacc aatcaccatc ggcgggcaag cattaggtcc agcgtcatta accactttca
     3181 ttacaaaatc tttaactccc tgcaatccat cacttggttt taacatttta atttgtgaca
     3241 tgttttcaga cccaaatcct tttggagcta caacaatttt aaattcatca ccacaaacaa
     3301 gatcaaaata aataattgct ggggtattat ctttagtatt gatccgttca aataatggat
     3361 catcaactac tgatttacga agataacctt cctcgtaccc ttgacgcact ccttcattaa
     3421 tagcagcttt taaatcacca tccacatgaa cttcttgtcc aattgtaata tacacaaaag
     3481 ccataccagt atcttgacaa atcggcatca tattatcttt agctaaatca gcattatcaa
     3541 tcaatacttc caaagtcttt ttagctaagg gatatggttc tgtatctgtc ttatctttaa
     3601 gtgccttaaa cacatcactg ggtaaattac aagctgcttc catacatagt tgttttactg
     3661 tttttgtaat atcttcacat ttaatctcac gcataacttt ttccttccat aaaaaaaggt
     3721 taaaaccttt ttagataatt ttttcctgaa ttcctggata atataaagaa taatatcgca
     3781 gtgacatcaa ttcaactgct gttaataacg tatgtttacc aatctttcgc tgttcgctat
     3841 cacctaatac aatcgtataa ttaacgttta ttccaatatc tatccctgga ttatttgtca
     3901 ttaaaacaac cttacatttt gatttcttta aatactgtaa aactttcgaa ttactccgtg
     3961 cataaaatcc atttactgtc acaataattg caacactatt ttcatccatc aaacgcgcac
     4021 aatcaatctg acgttctgtt tccatatatg ccagtgtaaa cttttccagc attagtaaat
     4081 cagtttgaaa atgcaacgct gcagaatgag aaaactgagt tccaaaaaaa gctactgaat
     4141 cactatcatg aatagcctgt agaactttat caatcacatt ccagtctaac cgctgtgaca
     4201 attttgaaat agcatcacat acttgatcac tataatattt agtacattct tgaggtttat
     4261 ctttcatcat gtccaaagga acattaatta aattattaaa ctttttgcta tctgaagcaa
     4321 atgaagaaca ttcttgtttt aaatgagcat aattttcata tcctaaagca cgacaaaaac
     4381 gtgaaatcgt tgccggggat acataacatt ctttagcaag ctgactaatt cccattttgg
     4441 ccactttcga aaaattatga gccatatacc aggcaatatt ataatttgta tcagcttcac
     4501 tagctgtatc taaaaagata attaaacgat aaaataagct tttcatcctc attcctccac
     4561 ttatgtgtat tatacactat atttcgccat tttaaaatag ctcgatatct atccgaacga
     4621 ataattatca catattttaa gatattctat atacattttc accattgttt agttatatac
     4681 taacaatgta gtcaaaagaa atattcgatt ttcccttcaa tataatgact acaaacaata
     4741 tataaagata tccttccctg atatcatgta ttattagtat cctttccctg ataactaata
     4801 ataaaagcga caagtgattt ccccacttgt cgtttttctg tttctataaa atttgactat
     4861 aaacacgtaa aacgttctta taaaaaactt tctcaatttc attttcacta aagccagctt
     4921 cctgcagtgc agcagctaat tggggcatca tcgaagcatc agctaattca aggttttgat
     4981 caataccatc a
//
LOCUS       DAOMTA010000116         4979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_393_length_4979_cov_2.470959, whole genome shotgun sequence.
ACCESSION   DAOMTA010000116 DAOMTA010000000
VERSION     DAOMTA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_4979_cov_2.470959"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..584)
                     /locus_tag="PLK68_04460"
     CDS             complement(42..584)
                     /locus_tag="PLK68_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89696.1"
                     /translation="MITVANSAEYFINEFTQEIYDQVLDHVDFKSLECSCGAKGSFVK
                     IGCYPRFYKTATNKICIRIQRVMCKHCGKTHAVFVECMVPSSMLLLTTQIEMLRSYYN
                     HRLEEFLSSYPTIDRPNAIYVIKNYERKWVNYLKKSGFTLKSKEREIQRYFFEKYQVQ
                     FLQMKCFSNSSSSRLLNHLV"
     gene            complement(784..2070)
                     /locus_tag="PLK68_04465"
     CDS             complement(784..2070)
                     /locus_tag="PLK68_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HRM89697.1"
                     /translation="MSVFKVIVDIFQNYVFNQPFIFLSIVAMIGLILQKKSIDKIISG
                     SVKTGIGYLILSVGTSTIAGVVTPIATLLEKIMGIETVATGMGTNAFTEQWASTIAII
                     MVIGFFINLILARFTPFKYVFLKAHQTYYLIFVYLAVAVEVFANPNNTLVIIIGGLLT
                     GIYCTLAPALAQPFLRKVTGSDDFAYGHTTTFGVITGSLVGNLFKKHKNESSENININ
                     PKLNFLKDITVSTALVMTILYIVAVCLAGFDYVEANLSNGQPAILYAIVSGVQFGVGI
                     TIVLNGVSMMVSEITEAFKGISEKIVPNAVPALDCPVVFNFAPTAVMLGFLSCLGTVI
                     LCTIIFGAIGWYALTPPVITTFFGGGPAGVFGNSTGGWRGAILAGVVAGLLLSFGQAL
                     TVGVLSTTVADFARWSNDFDYSVFPAFFKWILQLFA"
     gene            complement(2083..2379)
                     /locus_tag="PLK68_04470"
     CDS             complement(2083..2379)
                     /locus_tag="PLK68_04470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM89698.1"
                     /translation="MKSFKVITVCGAGVGTSTLLRMNIDKTFKKFALPLEVTVENKGL
                     SMSKGLMCDAVFTFESFADELRSCYEDVIVINNLMDMNELEEKIKKLLEKKNLL"
     gene            complement(2383..>3314)
                     /locus_tag="PLK68_04475"
     CDS             complement(2383..>3314)
                     /locus_tag="PLK68_04475"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HRM89699.1"
                     /translation="QNSISLVTFKNGVKFGHSVNDPVRVLLALAAKSDEEHLKLFQEV
                     ALCLMDRKYLHKIFNARSYQDILKDNKNIDAIKGGLIVSCYADSAINPYMDNSIAIQC
                     LAQSCVAGGAKAIRTNLEHVKAIAEVVDVPLIGIKKIYKGDDPLHSSFRITPTMDEVD
                     QLVAAGVDGIAIDGTQRERYDDLSLEEFVNKIKNKYPELFVIADISTVEEGIRASKAG
                     VDAVGTTLSGYTPYSKNPIIFGTVPSPDPDYEIIKELKTAGVSRVIAEGRINDGSKMK
                     KCIEAGAFAVVIGTSISEPAKIVKTILHDAKEG"
     assembly_gap    3315..3339
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3340..3527)
                     /locus_tag="PLK68_04480"
     CDS             complement(<3340..3527)
                     /locus_tag="PLK68_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM89700.1"
                     /translation="MIKYIKENCVDFYKECHDWKEAIVYAGYLLEKNGYIDHQYINDM
                     VNIIEKNGPYIVVMPGVA"
     gene            complement(3520..>4979)
                     /locus_tag="PLK68_04485"
     CDS             complement(3520..>4979)
                     /locus_tag="PLK68_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89701.1"
                     /translation="KKLTYLSYKDICIHLAVSLTRIELGYDIILHQDYLIEISKTEGY
                     HDAQFICSQIMQVLNIKMSQSEVAIITILLNSARYTTLKRYVIYDWANIQMIATTLAF
                     KVGEKLNVNFSRDEELINSLTLHLGPTVFKLQNQVPVVNPSLTVIKKNYHEVFIALLE
                     TIDELGYKELKGIKEDDIAFLTLHFCAALERKNRFKDVYNVVIVCVHGFGTASLMKEM
                     ICSRFKNIIVKKTVTEERILNIDLSNIDFIISSIDLQIMQCLVVKVNVILKESDYRLI
                     ENAMEKIVHQNIQESDTFMDGILSIVKRNCEIVNYQQLFDDFSEYFKDLGIDAGIKLQ
                     PLLNEYLTADKVLLCDTVDHWETAVCCAGQMLVKSQDIAECYIKSMIDTVKKSGSYMV
                     IDEGIALIHGEIDYGVNQTSMSLLIIKNGVKFNHDKYDPVHIILCLAAKDNYTHMKAL
                     NNFIKFLENIRNNGIERYFELDYVLDQINEVSKYD"
BASE COUNT         1667 a          971 c          608 g         1708 t
ORIGIN      
        1 ccaatttcga acataaaact tatgtgtata tagcaaatta gctaaaccaa atggttcagt
       61 aggcgtgacg aggatgaatt tgagaaacat ttcatctgta aaaattgtac ttgatatttt
      121 tcaaagaaat atctctgaat ctctctttct ttacttttta gggtaaatcc agattttttt
      181 agataattta cccacttccg ttcataattt ttaataacat aaatagcatt tggacgatca
      241 atagttggat aactacttaa aaattcttct aaccggtgat tatagtaaga tcttagcatc
      301 tcaatttgtg tagtcagcaa aagcattgat gaaggtacca tgcactcaac aaaaactgca
      361 tgtgtttttc cacagtgttt acacatcaca cgctggattc gaatacaaat tttattagtt
      421 gcagttttat aaaatcttgg ataacatcca atcttaacaa aactcccttt ggccccacaa
      481 ctacactcca ggcttttaaa atcaacatga tctaacactt gatcatatat ttcttgagta
      541 aattcgttaa taaaatactc ggcactatta gcaactgtta tcattttctt tatcccctta
      601 aattatccct ttctttaaaa tatcctattt tcatcagaat tgcaattatt tagatagtca
      661 aagtttttta attaagttaa ttttaaggag ctaaatacac aaatataaaa gacagtcagt
      721 ttctttttct tttcttgaca atcttaataa acttaaaaag ggataatttt atcccttata
      781 attttaagca aacaactgta gtatccattt gaaaaacgct ggaaatacag aataatcaaa
      841 atcatttgac catctagcaa agtccgctac tgttgttgat aaaacgccta ccgttaacgc
      901 ttgtccaaat gacaataaca gtccagcaac gactccagct aaaatagccc cacgccagcc
      961 tcctgtagag tttccaaaaa cacctgctgg tccacctcca aaaaaggtag taataaccgg
     1021 tggtgtcaga gcataccagc caattgcacc aaagataatc gtacacaata taactgtacc
     1081 taaacaactt aaaaaaccta gcataactgc agttggagca aagttaaaaa cgacaggaca
     1141 gtctaatgct ggtactgcat taggtacaat tttttcagaa atacctttaa acgcttctgt
     1201 gatctctgaa accatcattg aaacaccatt caatacaatt gtaatcccca ctccaaattg
     1261 aacgccagaa acaatcgcat ataaaattgc gggctgtcca ttacttaaat tcgcttcgac
     1321 ataatcaaaa ccagccaagc atactgccac gatatatagg attgtcataa ctaaagcggt
     1381 agaaactgta atgtctttta aaaagtttaa tttaggatta atattaatgt tttcagacga
     1441 ttcattttta tgttttttaa ataaatttcc aactaatgaa cccgtaataa ctccgaatgt
     1501 tgtggtgtga ccataggcaa aatcatcact gccggtaacc tttctcaaga aaggctgggc
     1561 caatgctgga gctaaagtac agtagattcc tgttaataga ccaccaataa taataactaa
     1621 cgtattattt ggattagcaa acacttctac agctacagct aaataaacaa agatcaaata
     1681 atatgtttga tgtgctttta aaaacacgta cttaaatggt gtaaatctag ctaaaattag
     1741 atttataaaa aaaccaataa ccataataat tgcaatcgtc gatgcccact gttcagtaaa
     1801 tgcatttgtt cccattccag ttgcaactgt ttcaatcccc ataatttttt ccagcagcgt
     1861 agctatcggc gtaactacgc ctgcgattgt gcttgttcct acacttagaa ttaaataacc
     1921 gattcccgtt ttgactgatc cagaaataat tttatctatt gatttttttt gtaaaataag
     1981 accaatcatc gcaacaatac ttaagaaaat aaacggctga ttaaacacat aattttgaaa
     2041 aatatctaca ataactttaa atactgacat aatctccctc ctctacaata aattcttttt
     2101 ttctaataat ttttttattt tttcctctaa ttcattcata tccattagat tattgattac
     2161 aataacatcc tcataacaag aacgtaattc atcagcaaac gattcaaaag taaatactgc
     2221 atcgcacatt agtcctttag acattgataa tcctttattc tcaactgtta cctctaatgg
     2281 taaagcaaat tttttaaaag ttttatcaat attcattctt aacaaagtgc ttgttccgac
     2341 tccggcacca cataccgtga taactttaaa acttttcatt tttcatccct cttttgcatc
     2401 atgtaatatt gtttttacta ttttagctgg ctcacttatg gatgttccga taacaactgc
     2461 aaaagctcct gcctctatac atttcttcat cttactaccg tcattaattc ttccttcggc
     2521 aatgactctt gatactccgg ctgtttttag ctctttaata atttcataat ctggatctgg
     2581 tgatggcact gttccaaata taattggatt tttagaatat ggcgtgtatc ctgataaagt
     2641 agtacctact gcatcaactc ccgctttaga tgctctgatt ccctcttcca ctgtagaaat
     2701 atctgcaata acaaataact ctggatattt attttttatt ttattaacaa attcttctaa
     2761 tgataaatca tcataacgtt ctctttgagt gccatctatg gctataccat caacgcctgc
     2821 tgcaactagc tgatcgactt catccattgt tggtgtaatt ctaaaactag aatgaagcgg
     2881 atcatctcct ttatagattt ttttaatacc aataagcgga acatctacaa cttctgcaat
     2941 tgcctttaca tgttctaaat ttgttcttat cgccttagct cctccagcaa cacaactttg
     3001 agccaggcat tgaattgcaa tagaattgtc catataggga tttattgctg aatcagcata
     3061 acaagaaact atcagtcccc ctttaatagc atcaatattt ttattatcct ttaaaatatc
     3121 ttgataagaa cgggcattaa atattttatg taaatatttt ctatccatta gacatagtgc
     3181 cacttcttga aacagcttta aatgctcctc atcactttta gctgcaagcg ctaacaatac
     3241 tcgaactgga tcattaacag agtgtccaaa cttcactcca tttttaaaag ttactaagct
     3301 aatactattt tgatnnnnnn nnnnnnnnnn nnnnnnnnna aggcaacccc tggcatgact
     3361 acaatatagg ggccattttt ttcaataata ttcaccatat catttatata ctggtgatca
     3421 atatagccat ttttctctaa tagataacca gcatagacaa ttgcttcttt ccaatcatgg
     3481 cactctttat aaaaatccac acaattctct tttatatatt taatcatatt tacttacctc
     3541 attaatctgg tctaaaacat agtctagttc aaaataacgt tctatcccat tatttcttat
     3601 attctctaaa aatttgataa aattatttaa tgctttcatg tgcgtataat tatctttagc
     3661 cgctaaacat aaaataatat gcactggatc atatttatca tgattaaatt tgacaccatt
     3721 tttaataatt aacagactca ttgatgtttg attaacccca taatcaattt ctccatgaat
     3781 aagggctatt ccttcatcaa taaccatata tgaccctgat tttttcaccg tatctatcat
     3841 tgatttaata taacattctg caatatcttg actcttaact agcatttgtc cggcacagca
     3901 gactgcagtc tcccaatgat cgaccgtatc acatagtaga actttatcag cagttaaata
     3961 ttcatttaat aatggctgca gtttgattcc tgcatcaatt cctaaatctt taaaatattc
     4021 actgaaatca tcgaaaagct gctgatagtt tacaatctcg cagtttctct taacaatact
     4081 taaaattcca tccataaaag tatcactttc ttgaatattt tgatgaacaa ttttttccat
     4141 agcattttca atcaatcgat aatcactctc ttttaaaatt acattgactt taacaaccaa
     4201 acactgcatt atttgtagat caatacttga aataataaaa tcgatattac tgagatctat
     4261 atttaaaatc cgttcttcag ttactgtttt cttcacaata atatttttaa atctcgagca
     4321 gatcatctcc ttcattaaag aagccgtccc aaaaccatga acacaaacaa ttacaacatt
     4381 atacacatct ttaaaacgat tttttctctc taaagcggca caaaaatgaa gcgttaaaaa
     4441 agctatatca tcctctttaa ttccttttaa ttccttatag cctaattcat caattgtttc
     4501 taataatgct ataaaaactt catgataatt tttcttaata actgtcaaac tcggattaac
     4561 aaccggtact tgattttgca gtttaaatac tgtaggacct aaatggaggg ttaaagaatt
     4621 gatcaattcc tcatctcgac taaaatttac attaagtttt tctcctactt taaaggctaa
     4681 agtagttgct atcatttgta tattagccca atcatagata acatatcgct ttaaagtcgt
     4741 atatctagca ctatttaata agatcgtaat aatagcaact tctgattgtg acatcttaat
     4801 atttaatacc tgcattattt gtgaacaaat aaattgagca tcatgatatc cttctgtttt
     4861 tgaaatctca atcaaataat cttgatgtag gatgatatca taccctaatt ctattcttgt
     4921 caaagaaaca gctaaatgga tacaaatatc tttatatgat aaataggtta atttctttt
//
LOCUS       DAOMTA010000117         4969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_395_length_4969_cov_2.568580, whole genome shotgun sequence.
ACCESSION   DAOMTA010000117 DAOMTA010000000
VERSION     DAOMTA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_4969_cov_2.568580"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="brxC"
                     /locus_tag="PLK68_04490"
     CDS             complement(<1..1077)
                     /gene="brxC"
                     /locus_tag="PLK68_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRM89702.1"
                     /translation="MKIKNMFKDDINRSINGVIQVEQEENNVIKQEVEEYVITSELKK
                     HFNKFFSSYSDSFDHPTDNTGIWITGFFGSGKSHFIKMLSYLLENKNVDGKTTVEYFR
                     DKYNDELSFMPIVKSTSVPTETILFNIGAQSIGAKDETAVLRVFTRVFYDHLGFYGSD
                     VKLAKLEQFVTKQGKMDEFKAKFEEINGDDWISAREEYVFWEDDIIEVLQDVLGMSED
                     AAKHWFDGTEEVHISIDQLAKEVKEYVDSKPKGFRLLFMADEVGQFVGSNLNMLLDLQ
                     TIVERFGSVCRGQVWVVATGQEALDEMIKVRTDAFSRIMARFSVKLSLTSSSVGEVIE
                     KRLLSKTDEAYENLIDAINDNHIEI"
     gene            complement(1090..>1287)
                     /locus_tag="PLK68_04495"
     CDS             complement(1090..>1287)
                     /locus_tag="PLK68_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HRM89703.1"
                     /translation="GDVVLLSGVGKVFPFMRVHGLLETMQPLFNDVPILVLYPGEFDG
                     HYVKLFNRLKANEYYRAFKSI"
     assembly_gap    1288..1297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1298..1655)
                     /locus_tag="PLK68_04500"
     CDS             complement(<1298..1655)
                     /locus_tag="PLK68_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HRM89704.1"
                     /translation="MSNISERLDNFKELIQESDFLEGKGLSNEVNIRIFCYDPKEEMV
                     IRHFLKQLESDQLKCNLIHYDLYEVFLQICEDKRILPKIPALEEKRGKEFIRKQFEKS
                     CDAKTFASKMQYENHQL"
     gene            complement(1659..2267)
                     /locus_tag="PLK68_04505"
     CDS             complement(1659..2267)
                     /locus_tag="PLK68_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HRM89705.1"
                     /translation="MNINTPYKTITREQFLFHEMRITARLLLEDKTDKEIIDEIVDNN
                     LFQYPTERMIKNLAGVCLKRLHELDDMTLVDVLANGSSESAKQICLYAMMNYYHLVRD
                     FMTTVVAEKYSSKDFYFSKMDVNVFFTRLQEQNDVVATWSDSTVNKCKQVLVKVLVEN
                     EYLDSPRSETLNPVLIDYQLKNALIDKGEKASLAAFNCFEGE"
     gene            complement(2257..2484)
                     /locus_tag="PLK68_04510"
     CDS             complement(2257..2484)
                     /locus_tag="PLK68_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM89706.1"
                     /translation="MDVCYNKLWKLLIDKKMNKTTFAKEVGISANTLAKLSKDENVNM
                     AILAKICRYLECSVDDILDVLPEREIENSEY"
     gene            complement(2927..3553)
                     /locus_tag="PLK68_04515"
     CDS             complement(2927..3553)
                     /locus_tag="PLK68_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRM89707.1"
                     /translation="MGQAYNIDRVNIIKRNIKSMNYRYIAKSTFSNSNSRNTQFKYVN
                     FRGSHFKKVKFGNVIFQGCDFWGTSFNKCTFKNVQFIDCTFVGAIFKNSKQEKISIEY
                     CCIVNSKIDFTYNNIKSEVLLEYPSVVIDPALKNSIISLKDNLHLKKNKMLYISDKKL
                     NLLNIYLLKKRFGEKTVLLLQKLTEKNCKLITTYTALEKTLFLISKTI"
     gene            complement(3680..4792)
                     /locus_tag="PLK68_04520"
     CDS             complement(3680..4792)
                     /locus_tag="PLK68_04520"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM89708.1"
                     /translation="MSQKLDSILSTYSYDARQKNQLRLADEKGLDIDIIKDPRYDWEQ
                     MREIALAMEFGLDPAPLCNPNISSEAMENIRIHLFENQGVYENAKADIHLKRTRILTI
                     IIVITLLLSSIAALLYAKKDYIMAYYEDIDLVLTDDHITLSYDDTFHPIDYIKKYDEK
                     NTITLPKLQKMDQLTDYEYVYEVSNGVKTTTKTLIVTVIDDQKPTINLKQEEVTLTQG
                     DTFNPLDYVASVNDNYSKLKKEDITFEGNVDTNQQGKFTFQYNLKDSSNNKTTATLNV
                     TVKEKPKPVNQSPNTNNSNPTKTNQTTQHQQQSNKPKPSNKVFYIRDYNYDIASCKNA
                     AVSYMDSQLHSTSATYGELQPYQENGTTVGYKVVFR"
     gene            complement(4773..>4969)
                     /locus_tag="PLK68_04525"
     CDS             complement(4773..>4969)
                     /locus_tag="PLK68_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89709.1"
                     /translation="HAAYWTLIDRYYDEIEHREGLIRYLDYCKTHHIYHDYLIESCEC
                     ELPDIFDYVEEPYTYEPEIR"
BASE COUNT         1556 a          854 c          629 g         1920 t
ORIGIN      
        1 aatttcaata tggttatcat tgattgcatc aataaggttt tcatatgctt catctgtttt
       61 agataataaa cgtttttcaa taacttcacc tactgatgat gaagttaaac ttaatttaac
      121 actaaatcta gccatgatac gtgagaatgc atctgtacgt actttgatca tttcgtctaa
      181 tgcttcttgt ccggttgcta caacccatac ttgtcctcta catactgaac caaaacgttc
      241 tacaatagtt tgtaaatcta ataacatatt taaattagaa ccaacgaact gaccaacttc
      301 atcagccatg aataataatc taaatccttt tggtttagaa tctacatact ctttgacttc
      361 ttttgctaat tgatcaatag aaatatgtac ttcttcagtt ccatcgaacc aatgtttcgc
      421 agcatcttca gacataccta atacatcttg taatacttca ataatatcat cttcccagaa
      481 cacatattct tctctagcag aaatccaatc atcaccgttg atttcttcaa attttgcttt
      541 gaattcatcc attttgcctt gtttagtaac gaactgttct aatttagcaa gttttacatc
      601 actaccatag aatcctaaat ggtcatagaa tacacgtgta aatacacgta atacggcagt
      661 ttcatctttt gcaccaatac tttgagcacc aatgttaaat agaattgttt ctgttggtac
      721 acttgttgat tttacaattg gcataaaaga taattcatca ttatatttat cgcggaaata
      781 ttctactgtt gtttttccgt cgacattttt attttctaat aagtaagata acatcttgat
      841 aaagtgagat ttcccacttc caaagaaacc agtaatccag atacctgtat tatctgtagg
      901 atgatcaaat gaatcactat agctgctaaa gaatttatta aagtgtttct ttaattctga
      961 tgtgataaca tattcttcta cttcttgttt aattacatta ttttcttctt gttctacttg
     1021 gataacacca ttgattgatc tattgatgtc atctttaaac atatttttaa tcttcatact
     1081 cttctactcc taaattgact taaatgctct atagtattca tttgctttta atctattaaa
     1141 tagttttaca tagtggccat caaattcacc tgggtataaa actaatattg gtacatcgtt
     1201 gaataatggc tgcattgttt ctaataaacc atgcactctc ataaacggga atactttgcc
     1261 caccccagat aacaagacta catcaccnnn nnnnnnncta attgatgatt ttcatattgc
     1321 atcttactag caaaggtttt agcatcacag cttttttcaa actgttttct aataaattct
     1381 ttacctcgtt tttcttccaa tgctggaatc ttaggtaaga ttcttttatc ttcacatatt
     1441 tgaagaaata cctcatataa atcatagtga attaaattgc actttagttg atctgattct
     1501 agctgtttca agaaatgtct gattaccatt tcttcttttg gatcataaca gaagattcta
     1561 atattaactt cattgcttaa tcctttacct tcaagaaaat cactttcttg aatgagttct
     1621 ttgaaattat ctaatctttc actaatatta ctcatcttct actctccttc aaagcagtta
     1681 aaagctgcta agcttgcttt ttcaccttta tcaataagtg cattttttaa ttgataatct
     1741 attaatactg gattcaatgt ttccgatcta ggtgaatcta aatactcatt ttcaactaac
     1801 accttcacca atacttgttt acatttatta actgtgctgt ccgaccaagt tgcaactaca
     1861 tcattttgtt cttgtaatcg tgtaaagaaa acattcacat ccatttttga aaagtaaaaa
     1921 tcctttgaac tatacttttc agcaacaact gttgtcataa agtcccttac taagtgataa
     1981 taattcatca ttgcatatag acaaatttgt tttgcacttt cactagatcc attggcaagg
     2041 acatccacca acgtcatatc atcaagctca tgtaatcttt ttaaacatac accagctaaa
     2101 ttcttaatca ttctttctgt tggatattgg aataagttat tatcgacgat ttcatctatt
     2161 atttctttat ctgtcttatc ttctaataat aatcgagctg taattctcat ttcgtgaaat
     2221 aagaattgct ctcttgtaat tgtcttatat ggtgtattaa tattcactat tttcaatctc
     2281 tctttcaggt aagacatcta gtatatcatc cacactacat tctaaataac ggcagatttt
     2341 tgctaatata gccatattta cattctcatc ttttgataat ttagctaatg tattggcact
     2401 aatgccaact tctttagcaa aagttgtctt attcattttc ttatctataa gtaacttcca
     2461 taatttgtta taacaaacat ccatatttga tacctccaac tgtttaagca aatattattt
     2521 tatcatattt tactatatat tgagaagaga tattggtata caccaatatt tattttattt
     2581 ttctaatatc cattatagta aaaaaatcca atgttaagta tgctaacagc atacttaaca
     2641 ttggttgtta aacgattcat atatatacat atctaattat gcaacatttt atttataatc
     2701 aataaacgca aataacaatt catcttaata agaccccaaa caatcaagag atgactttgt
     2761 aagaatttgt aaacctaaaa aagagataaa cccaataata gttgccaaat catttaccat
     2821 agacatccct ccttgaatgt tcgctatcat gttacagtcc ccactcactt ataaaaagta
     2881 tcattagcat ctcaatctct cgattgagtg gggcaggaca tgaatattat atagttttac
     2941 tgatcaaaaa taaagttttt tctagtgcgg tgtatgtagt tatcagtttg caattcttct
     3001 ccgttaattt ttgtaataat aaaacggttt tctccccaaa acgtttttta agtaaatata
     3061 tattaagtaa gttcaacttt ttatccgaga tataaagcat tttatttttc ttcaaatgaa
     3121 gattgtcttt caaagatata atactgttct ttagcgctgg atcgattact actgatggat
     3181 actctaataa cacttctgat ttgatgttgt tatacgtaaa atctatcttt gaattcacga
     3241 tacaacaata ctcaatagaa atcttttctt gtttagaatt tttaaagatt gcgcctacga
     3301 atgtacaatc gataaattgt acattcttaa aagtacactt attaaaagac gtcccccaaa
     3361 aatcacatcc ttgaaatata acattgccaa atttcacttt cttaaaatgt gatcctctaa
     3421 aattcacata tttaaattgc gtatttcgag agttagaatt actaaatgta ctcttagcta
     3481 tatacctata attcattgat tttatatttc tcttaatgat attaactcta tcaatattat
     3541 aagcctgtcc catagtttcc cttctttcta tttgcgttaa gttattgcaa tgaacaaaaa
     3601 cactatgatg attaatttat gtaccactat gcctaaagcc aattatatta tttttctatt
     3661 gctaatacta taggcttact tacctaaata caactttata cccaacagta gttccatttt
     3721 cctgatacgg ttgtaattca ccatatgtag ctgatgttga atgtaactga ctatccatat
     3781 aagaaacagc tgcattctta catgatgcaa tatcgtagtt atagtctctg atatagaata
     3841 ctttatttga tggttttggt ttgtttgatt gttgttgatg ttgtgttgtt tgattggttt
     3901 tagttggatt ggaattattc gtatttggtg attgatttac tggttttggc ttttctttaa
     3961 ctgttacatt tagtgtagca gttgttttat tatttgaaga atcttttagg ttgtattgga
     4021 aggtaaattt accttgttga tttgtatcaa cattaccttc aaaagtaata tcttcttttt
     4081 ttaacttgct gtagttatca ttaacggatg ctacataatc taatggattg aaggtatctc
     4141 cttgtgttaa agtgacttct tcttgtttta gattaattgt tggcttttga tcatctataa
     4201 ctgtaacaat taaagttttt gtagttgtct ttactccatt actaacctca taaacatatt
     4261 cataatcagt aagctgatcc attttttgaa gctttggtaa cgtgattgta ttcttttcat
     4321 catatttctt tatgtagtca attggatgaa aggtatcatc ataggataag gtaatatgat
     4381 catcagtaag tactaaatca atatcttcat aataagccat aatgtaatct ttctttgcat
     4441 ataataaagc agctattgat gataataata gtgtgataac aatgatgatg gttagtatcc
     4501 tggttctttt taaatgaata tcagcctttg cattttcata gacaccttga ttttcaaata
     4561 aatggattct aatgttttcc attgcttctg atgaaatgtt aggattacac aatggagctg
     4621 gatcaagtcc aaactccatt gctaatgcta tttctctcat ttgttcccag tcatatctag
     4681 gatctttgat gatatctata tccaatcctt tttcatcagc aagtcttagc tgattttttt
     4741 gtcttgcatc ataactgtag gttgaaagaa tactatctaa tttctggctc ataggtatat
     4801 ggttcctcaa cataatcaaa gatatctggt agttcacatt cacatgattc tattagataa
     4861 tcatgataga tatgatgagt tttacagtaa tctagatatc tgattaatcc ttcacgatgt
     4921 tcaatttcat cataataacg atcaattaat gtccaatagg ctgcatgag
//
LOCUS       DAOMTA010000118         4902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_413_length_4902_cov_2.378791, whole genome shotgun sequence.
ACCESSION   DAOMTA010000118 DAOMTA010000000
VERSION     DAOMTA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_4902_cov_2.378791"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..561
                     /locus_tag="PLK68_04530"
     CDS             142..561
                     /locus_tag="PLK68_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89710.1"
                     /translation="MRIDFFSTSEGMDQRAANHGVYSIELVGRGKKICLYIGESVWIA
                     SRCGVHLYELYEDPGYFGLTKDDLNNDEFTLKFSVIESISDKKTLLGIGEYKQLELEA
                     IKSNRPLTQLETSDRQIRNKEEKIKKVQGELLNQGFK"
     gene            625..1407
                     /locus_tag="PLK68_04535"
     CDS             625..1407
                     /locus_tag="PLK68_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89711.1"
                     /translation="MYTVYIPKYKGENPDFNIIKVIKLYMRSTDYDCGILSAPGYMNK
                     NVNTTDMYFTKTGKIFHKSRLGKNRNVSVGIFNGVNGTTLINVGKKPGKITCREYHEN
                     SIKSHGLNLIRIKCKKSSDHRKMIFFLENPDMRFISVEDMEKRDESRNEELSLANYSD
                     FLTNTKVRAIMIGSSNQSLQTYYGGSKGLSADKGEADVFMFVDDSDVKDAFVNTDDKL
                     LQDSIVISKSIKVPGNLGNERVADEKYLKSILEDFLKNSLEQ"
     gene            1437..2306
                     /locus_tag="PLK68_04540"
     CDS             1437..2306
                     /locus_tag="PLK68_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89712.1"
                     /translation="MSIADIIQIILSGLSLLATIAVSFSIYYLQRKHEKEIQKIEENL
                     QKRKLKEKAELFIINNNSEIDYLPWCIFASNLHKLETHHRKIYKEYCKCSLEVQNEIL
                     RIQGFEVKQLDENNDWLDKCLELLRKDILEHKLGRDVLYDGAKYFKRGYRIYKRLEWG
                     DIEYSNIFRPIEENWGFKKTNLRNCTSYIEEYLYFLYSDSKPVLYNSSPLPPIDYVCE
                     AIGFENNKEEIVCAWIMELVQAISINLLNNRFVNSRENSQKIKYTDATIETYEYKYYS
                     AIHALYNLYFNCK"
     gene            2683..3150
                     /locus_tag="PLK68_04545"
     CDS             2683..3150
                     /locus_tag="PLK68_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM89713.1"
                     /translation="MFSIFSKKVDNNLYAPVKGKCIDITDVSDAGFSSKIMGDGIAII
                     PDDNIICSPCDGELKMMFHTGHAFGITMKNGLEILVHIGIDTVNLDGKGFKILKKSNS
                     MVKKGEPIVEIDLDLIKKEYDPSTMIIITNKKSFEKLKVNAVVDLHDKIIKAE"
     gene            3153..3980
                     /locus_tag="PLK68_04550"
     CDS             3153..3980
                     /locus_tag="PLK68_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89714.1"
                     /translation="MKLIKKLNNNYALALDTNGENIIVEGKGIGFIRMPCELEDLSII
                     SRAYYDTKTQDIELVKNIPEEVLSICERVVDFVSTKIGDKLNPNLTFILADHIEFCIE
                     RYENNIEVKMPIYYDIEYLYPAESAAADFALKLILEDMNVLLPESEKTGIALNIINSE
                     LKLVDESETNHEFVELCISIVEKVLEIEIEKTSFNYVRFVTHLEYLSKRIYDKKNIDT
                     ENTQLYEWVSNNYPEIKKCVDVICQVLKKKKIYMNREEKLYLMLHINRVCSREDCNH"
     assembly_gap    4145..4154
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4173..>4902
                     /locus_tag="PLK68_04555"
     CDS             4173..>4902
                     /locus_tag="PLK68_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HRM89715.1"
                     /translation="MNGTIIDMNNQELAKKILENIGGETNVSHATYCLTRLRITYKDR
                     GLIKDDEIRAVPGLIGTKYLGTQYQIIIGPGVESVYKEFCKLAHLEQKPEVDVNGNYL
                     TEETKEKFSLKRLGNKVLETIAACVTPMISIITLAGLLKMIVAILGPNMLNFMDPTSD
                     LMQLLSFVGDAGFYFMPVFAAYASAKRFGTNIQLSLFLACVLLHPTLIEIVKAGNPFT
                     VYGIPMTLTSYSTSFIPILLIVWAQ"
BASE COUNT         1931 a          514 c          893 g         1554 t
ORIGIN      
        1 tttcattaat attagatttg acaggaataa agaaaaataa actataatta ttagttgata
       61 acttattctt tgaaaaaaat gacgtaattg tttgttcatg ttataatttt aacgaaaact
      121 aggatttaag gaggcaatac attgagaata gattttttta gtacttctga gggcatggat
      181 caacgagctg caaaccatgg tgtatattcg atagaattag tgggcagggg taaaaagatt
      241 tgtttatata ttggggaatc ggtctggata gcatctagat gcggagtaca tttgtatgaa
      301 ctatatgaag accccgggta ttttggatta actaaagatg atcttaataa tgatgagttt
      361 actttaaagt ttagtgtcat agaaagtatt tctgacaaaa aaactttact aggtattgga
      421 gaatataagc agttggaact ggaagccatt aaaagcaata gaccactaac acaactggaa
      481 acgagtgaca gacaaataag aaataaagaa gaaaaaataa aaaaagtcca aggtgaatta
      541 ttaaatcaag gatttaaata gacaaattaa gtataaaaca atttttatat agtcagtagg
      601 cattaattat gagggagaga aaagatgtat acagtatata taccaaaata taagggagaa
      661 aatccagatt tcaatataat taaagtaatt aagttatata tgcgttcaac agattatgat
      721 tgtggtattt tatcggcacc agggtacatg aataaaaatg taaatacgac agatatgtat
      781 ttcactaaaa cggggaaaat atttcataaa tcgaggcttg gtaaaaatcg aaatgtttct
      841 gttggtattt ttaatggggt gaatggtaca actttaataa atgtaggtaa aaaacctgga
      901 aagattacat gtagagagta tcatgaaaat agtataaagt cacatggatt gaacttaata
      961 agaataaaat gtaaaaaaag cagtgatcat cgaaaaatga tatttttttt agaaaatcca
     1021 gatatgcgat tcatatctgt tgaagatatg gagaaaagag atgaaagtag gaatgaagaa
     1081 ttatcattag cgaactatag tgacttttta actaatacaa aggttagggc tataatgata
     1141 gggtcaagca atcaaagtct tcaaacatat tatggaggtt caaaagggct gtcagcagat
     1201 aaaggggaag cagatgtatt tatgtttgtt gacgattcag atgttaaaga tgctttcgtt
     1261 aatactgatg ataaattgtt acaagatagt attgttattt caaaaagtat aaaggttcct
     1321 ggaaatttgg gaaatgaaag agtggcggat gaaaaatatc tgaaatctat tcttgaggac
     1381 tttttgaaaa acagtttaga acagtaaatg aattataagg aaaggagatt taaattttgt
     1441 caatagcaga tataatacaa attattttaa gtggtctatc cctactagca actattgcag
     1501 tttctttttc aatatactat ctacaaagga aacacgaaaa agaaatacaa aaaattgagg
     1561 aaaatttgca aaaaagaaag ttaaaagaaa aagcagaact tttcattatt aataataatt
     1621 ccgaaattga ttatttgccc tggtgtattt tcgcttcgaa tcttcataaa ttagaaactc
     1681 atcatagaaa aatatacaaa gaatattgta aatgttctct ggaagtacaa aatgaaatac
     1741 taagaataca aggatttgag gtaaaacagt tagatgaaaa caatgactgg ttagacaaat
     1801 gtcttgaatt gttaagaaaa gatattcttg aacataaact aggtagagat gttttgtacg
     1861 atggagcaaa atattttaaa agaggttata gaatatataa gagattagaa tggggtgata
     1921 ttgaatactc aaatatcttt agacctatag aagaaaattg gggatttaaa aaaacgaatt
     1981 taagaaattg tacaagttat atagaggaat atttgtattt tttatatagt gattcaaaac
     2041 ctgtattata taattcgtct cctttacctc caattgatta cgtgtgtgag gctataggct
     2101 ttgaaaataa taaagaggaa atagtttgtg cctggataat ggaattagtt caggcaatat
     2161 ctattaatct actaaacaat agatttgtta actctcgtga aaatagtcaa aaaataaaat
     2221 atacggatgc aacaatcgaa acatatgagt ataaatatta cagtgcgatt catgcgctat
     2281 ataatttgta tttcaattgt aaatagtatt agaattttaa atattgtaaa aaaaaagatt
     2341 aatcgaatta ttaaagtgta cattgtattt ttgtagatag tgataattga ttacgatagt
     2401 tcaggtatga atatagggat attagtagat tttaataaga cagctaaaat agtaattgat
     2461 gtctagtaat ataaatctta taaaagcttg acaatattaa cggttagtag tagactaaaa
     2521 gtgtaataga tatggactgt aactcttatt gaggcataac cattcaattt taattgatga
     2581 ttatgcttag atgagagttt ttaatataaa tatttatatt gaaaatcttc attaatcata
     2641 attatcatat gtttatatta tttttgaaat ggagaatttg aaatgtttag tatatttagt
     2701 aaaaaagtag acaataatct atatgcgcct gttaaaggaa agtgtataga tattacagat
     2761 gtaagcgatg caggtttttc ctcgaaaata atgggagatg gaatagctat tatcccggat
     2821 gataatataa tctgttctcc ctgtgatggt gagttgaaaa tgatgtttca tacaggacat
     2881 gcctttggca taacaatgaa gaatggtttg gaaattcttg tacatattgg aattgataca
     2941 gtaaacctgg atgggaaagg atttaagatt ctaaaaaaat caaattcaat ggtaaagaaa
     3001 ggagaaccaa tcgtagaaat agatttagat ttaataaaaa aagaatatga tccttcgaca
     3061 atgattatta ttacaaataa aaagtcattt gaaaaattaa aagttaatgc ggtagtagat
     3121 ttacatgata aaattatcaa ggcggaataa aaatgaaact tataaaaaaa ctcaacaata
     3181 attatgcact ggctctagat acaaatggtg aaaatattat tgttgaagga aaaggaatag
     3241 gatttatcag gatgccatgt gaattggaag atctgtcaat tatttccaga gcttattatg
     3301 atactaaaac acaggatata gaactggtta aaaatattcc tgaggaagta ttatctatat
     3361 gcgaaagagt ggtagatttt gttagcacta aaataggcga taaattaaat ccaaatttaa
     3421 catttatttt ggctgatcac attgaattct gtattgaacg atatgaaaat aacattgagg
     3481 ttaaaatgcc gatttattat gacatagaat acttgtatcc tgccgaatca gctgccgcag
     3541 attttgcctt gaaactgatt ttggaagata tgaatgtact gcttccagaa agtgaaaaaa
     3601 caggtattgc attaaatatt attaattcgg aattgaaact agttgatgag tcagaaacga
     3661 atcatgaatt tgttgaattg tgtatttcaa tagttgagaa ggtattagaa attgaaattg
     3721 aaaaaacaag ttttaattat gttagatttg taacacatct tgaatattta tctaagcgga
     3781 tttacgataa aaaaaatatt gatacagaaa acacacaact ttatgaatgg gtaagcaata
     3841 attatcctga aataaaaaaa tgcgttgatg taatatgcca ggttttgaaa aagaaaaaaa
     3901 tatatatgaa tcgagaagaa aaattgtacc tgatgctgca tattaacaga gtatgttctc
     3961 gagaggactg caaccattaa atgggcataa cctcatctaa aaatagagtg atctatttta
     4021 aggtgaggtt ttattattag aagataaatt ataaatatat gaatattatt taaagaatat
     4081 ggttaattga gttaaaaaat gataaataat acgcgcggtt tattgttttg aatataaata
     4141 aaggnnnnnn nnnncctgta aatcacgcga taatgaatgg tacaattata gatatgaaca
     4201 atcaagaact agcaaaaaag attttagaga atattggcgg agagacaaat gtaagtcacg
     4261 ctacttattg cttaacaaga cttagaatta catataagga taggggacta attaaggatg
     4321 atgaaattag agcagttcct ggacttatag gaactaaata tctgggaaca caatatcaga
     4381 ttattattgg tccaggtgtt gaaagtgtat ataaggaatt ttgcaaatta gcacatttgg
     4441 aacaaaaacc agaagtggat gtaaatggaa attatttaac agaagaaaca aaagaaaaat
     4501 tttcattaaa aagattaggt aataaagttt tagagactat tgctgcttgt gtaacaccaa
     4561 tgatttcaat aattacatta gctggacttt taaaaatgat cgttgctatt cttggaccaa
     4621 atatgctaaa tttcatggat ccaacaagtg atcttatgca gttgttaagc tttgtcggtg
     4681 atgctggatt ctattttatg cctgtattcg ctgcgtatgc ttcagcaaaa cgttttggaa
     4741 caaatataca actatcttta tttttagcct gtgtactgct acatcctaca ttaattgaaa
     4801 ttgtgaaagc tggaaatcca tttactgttt acggtatacc tatgacatta acatcatatt
     4861 caacaagctt tattccaatt ttattaatag tatgggctca at
//
LOCUS       DAOMTA010000119         4900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_414_length_4900_cov_2.365325, whole genome shotgun sequence.
ACCESSION   DAOMTA010000119 DAOMTA010000000
VERSION     DAOMTA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_414_length_4900_cov_2.365325"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="recA"
                     /locus_tag="PLK68_04560"
     CDS             complement(<1..967)
                     /gene="recA"
                     /locus_tag="PLK68_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRM89716.1"
                     /translation="MAEAKTKENKEEAKKVQALDDAIKQIEKKYGKGSVMKLGDRAAV
                     SVDVIPTGSLTLDLALGIGGYPKGRIIEIYGPESSGKTTLTLHAIAECQKQGGRAAFI
                     DAEHAIDPVYAQNLGVNIDELILSQPDSGEQGLEIAETLVRSGAIDLVVVDSVAALVP
                     QVELDGEMGDSQMGLQARLMSKALRKIAAELNKSECTIIFINQLREKIGIMFGNPETT
                     TGGRALKFYSSVRVEIRRSEAIKLGTEIVGNKVNIKVVKNKVAPPFKTTQVDIIYGKG
                     ISRDGEVLDLAVEKDIVEKSGAWYAYKGEKIGQGRENAKTFLSTHS"
     gene            complement(1006..1464)
                     /locus_tag="PLK68_04565"
     CDS             complement(1006..1464)
                     /locus_tag="PLK68_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRM89717.1"
                     /translation="MDELAKTLIQYNISISSVESFTVGNFAAMLGSIPGISKVYKGSL
                     VTYQSTTKERLLGISHEIISKYGVVSKEVASLMCVNGKQILDSDVCVSFTGNAGPDAM
                     EGKPVGLVYIGILYQGVNIYELNLKGTREEIQKQAIDFVIRKLNEKIKVN"
     gene            complement(1464..2057)
                     /gene="pgsA"
                     /locus_tag="PLK68_04570"
     CDS             complement(1464..2057)
                     /gene="pgsA"
                     /locus_tag="PLK68_04570"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRM89718.1"
                     /translation="MNLPNKLTVTRVLLVPFLILIYMFPYDSAGITVPVYHVLETNIS
                     LVNIIILFIFIIASITDYFDGKIARKEKLITTFGKFADPIADKLLINTIFLLLASDQT
                     INIIIPIIMISRDTIVDAIKMSAASKQVVVAASKLGKLKTVSQMIALGFLLVNNFPFT
                     VLGVDVANALAWLATVISVISGIDYFLKNREMLTETM"
     gene            complement(2057..3049)
                     /locus_tag="PLK68_04575"
     CDS             complement(2057..3049)
                     /locus_tag="PLK68_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89719.1"
                     /translation="MENISEHVRREREARGVTIEELAKGTFISVAVLKDIESGKFDKY
                     KGDELYLKMYLRKIAKYLGLEEKELDAEFEALTQEIQLEEIRQEDLNKRLVEENKKNI
                     TISGKVSDTFKDLKNVKPKKSISNKRVYEDRYLLRYFKYALVGVVCVAIIFVVWYAIV
                     AGRSNTEAPDFKDNNTPTVEGNNDAGKQDSDDNKNSNTDDKNEDDKKAETPTVEITKN
                     GELDYSIKLDPSMTTFKFKMEFVGRTWSQLNVNGSDYSGFKSGIYNNANKSNATDAAP
                     EIVELDIPVENFQNLELKLGYFMGHRFYINDQPLEIDASEYDGGNHTLKITRVQ"
     gene            complement(3432..4577)
                     /gene="fucO"
                     /locus_tag="PLK68_04580"
     CDS             complement(3432..4577)
                     /gene="fucO"
                     /locus_tag="PLK68_04580"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HRM89720.1"
                     /translation="MANRFILNETSYHGSGAIREIVTEVKARGFKKAMVCSDPDLIKF
                     GVTKKVTDLLDAANLDYVVYSDIKPNPTIENVQSGVKALQEAKADYMIAIGGGSSMDT
                     AKAIGIIDKNPEFSDVRSLEGVAETKNPCTPILAVPTTAGTAAEVTINYVITDAQKDR
                     KMVCVDPHDLPVVAFVDPDMMASMPKGLTAATGMDALTHAIEGYITAGAWEMSDMFHI
                     KAIEIIARSLRGAVENTPEGREGMALGQYIAGMGFSNVGLGIVHSMAHPLGALYDTPH
                     GVANAIILPTVMEYNAPATGTKYKDIAEAMGVDTTGMDQEAYRKAAVDAVKKLSQDVG
                     IPADLKEIVKVEDLDFLSQSAYDDACRPGNPRETSVAEIKELYQSLL"
     gene            complement(4718..>4900)
                     /locus_tag="PLK68_04585"
     CDS             complement(4718..>4900)
                     /locus_tag="PLK68_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRM89721.1"
                     /translation="VRTVVTADIDFLLKMYCKGSIDMTVEWVLGDMPISIDDIVKLLI
                     EALPQRLEPFILNIKK"
BASE COUNT         1565 a         1006 c          613 g         1716 t
ORIGIN      
        1 cactatgagt acttaagaaa gtcttagcat tttcacgccc ttgacctatt ttttcacctt
       61 tatacgcata ccatgctcca ctcttttcaa caatgtcttt ttctactgcc aaatcgagta
      121 cttcaccatc acgagaaatt cctttaccat aaataatatc tacttgtgta gttttaaacg
      181 gtggagcaac tttattttta acaactttaa tattcacttt atttccaaca atttctgttc
      241 ctaatttaat tgcttcacta cgacgaattt cgacccgaac agaagaatag aattttaatg
      301 ctcttcctcc tgttgttgtt tctggattac caaacataat cccaatcttt tctctcaatt
      361 gattaataaa aataatcgta cattcagatt tatttaattc tgccgcaatt tttcttaacg
      421 ctttagacat caaacgtgct tgaagtccca tttgcgaatc acccatttca ccatcaagtt
      481 ctacttgagg cactaatgcc gcaaccgaat cgacaacaac taaatcaatc gcacctgaac
      541 gaaccaaagt ttcagcaatt tctagtcctt gttctccaga atcaggctgt gaaagaatta
      601 attcatcaat attaactccc aaattttgcg cataaacagg atcaatagca tgttctgcat
      661 caataaaagc agcacgtcct ccttgttttt gacattcagc aatggcatgt aatgtcaatg
      721 ttgttttacc actagattca ggtccgtaaa tctcaataat tcttccttta ggatatccgc
      781 cgattcctaa agctaaatct aaagttaaag aaccagtagg aattacatca acactaacgg
      841 cagcgcgatc acctaatttc attacagaac cttttccgta ttttttttca atttgcttaa
      901 ttgcatcatc caaagcttgg acttttttag cttcttcttt attctcttta gtctttgctt
      961 ctgccataat attcatcctc ctactattat atacctatat aaatactaat ttacttttat
     1021 tttttcattc aattttcgaa ttacaaaatc aattgcctgt ttctggattt cttcacgagt
     1081 tccctttaaa ttcaattcat agatattgac accttggtat aaaattccaa tataaactaa
     1141 tcctactggc ttcccttcca ttgcatcagg cccagcattg cctgtaaatg atacacagac
     1201 atcactatca agaatttgtt ttccattaac gcacatcaac gaagctactt ctttacttac
     1261 aactccatat tttgaaatta tttcatgcga aattcctaat agtctttcct ttgttgtact
     1321 ttgatatgtt actaaacttc ctttgtaaac cttagaaatg ccaggaatac ttcctagcat
     1381 tgcagcaaag ttacctactg taaaactctc tacactagat atacttatat tatattgtat
     1441 taatgtttta gctaattcat ccatcacatt gtctccgtaa gcatctcgcg attttttaag
     1501 aaataatcaa tcccgctaat aactgaaatt actgtagcta gccatgctaa agcgttagct
     1561 acatcgactc ccagtacagt aaaaggaaaa ttattaacta ataaaaatcc taatgcaatc
     1621 atttggctga ctgtctttag tttaccaagt ttacttgccg ccacgactac ttgtttactc
     1681 gcagctgaca ttttaatcgc atcaactata gtatcccgag aaatcattat aatagggata
     1741 ataatattga ttgtctggtc acttgccaat aataagaaaa tcgtattaat taacaactta
     1801 tcagcaattg gatcagcaaa tttaccaaat gttgttatta acttttcttt tcgagctatt
     1861 tttccatcaa aataatcagt aattgatgcg ataataaaga taaataaaat gataatattt
     1921 actaatgata tatttgtctc caaaacatga tagacaggaa ccgtaattcc tgcactatca
     1981 tacggaaaca tataaataag aattaagaag ggaactaaca atacccttgt aactgttaat
     2041 ttattcggta gattcattat tgcacccgcg taattttaag ggtatgatta cccccatcat
     2101 attcacttgc atcaatttct agaggttgat cgttaatgta gaaacgatgt cccataaaat
     2161 atcctaattt taattccaaa ttttggaaat tctctaccgg aatatctagt tcaactattt
     2221 ctggagctgc atcagttgca tttgatttat tagcattatt gtaaataccg ctcttgaagc
     2281 cgctataatc actaccattg acatttaatt gtgaccatgt ccgaccaaca aattccattt
     2341 taaacttgaa agtagtcata cttggatcaa gtttaatact ataatctaac tcaccatttt
     2401 tagttatttc aactgtaggc gtttcagcct tcttatcatc ttcattttta tcatcagtat
     2461 tagaattttt gttgtcatca ctgtcttgtt taccagcatc attatttcct tcgacagttg
     2521 gtgtattgtt atctttaaaa tctggcgctt cagtattaga acgccctgca acgattgcat
     2581 accaaacaac aaaaatgatg gccacacaaa ccacaccaac taaagcatat ttaaaatatc
     2641 taagcaaata gcgatcttca taaacacgtt tattagagat agatttttta ggttttacat
     2701 tttttaaatc tttaaatgta tcacttacct taccagaaat agtaatgttc tttttatttt
     2761 cttcaactaa ccgtttattt aaatcctctt gacgaatttc ttctaactga atttcttgtg
     2821 tcagtgcttc aaattcagca tctaattctt tttcttctaa ccctaggtat ttagcaattt
     2881 tccgtaaata cattttcaaa tataattcgt cacctttata tttgtcaaac ttacctgatt
     2941 ctatatcttt taagacagct actgaaataa atgttccttt ggccaactct tcaatcgtga
     3001 cccctctagc ctcacgttct cgtctaacat gttcactaat attttccatg agctatcctc
     3061 cttttttcca taattataaa aaagcctata ataaacacat aaattatatc ataattatta
     3121 tttatgacaa taacctaatc acatttattt tatgaaagat tttaaaaaaa tataccttaa
     3181 aaaaaccacc ttatatatga tagtttataa atataaacta tttataaact aattgaaatt
     3241 aattgagtaa tttttttaat ccatttatta gtctacaagt tgacaatata aacggtcaag
     3301 taatttttaa caaacacatt aaatcacaaa atcaaaaaaa ggctatggat tgatgtgacc
     3361 cccaaaagtt agctcaaaaa tctaactatc agaatactgg tacaaaactt acagcctttt
     3421 tctaatactt attataataa agattgatat aattctttaa tttcagcaac gctagtttct
     3481 cgtggattac cagggcggca agcgtcatca tatgctgatt gtgataaaaa gtcaagatct
     3541 tcaactttaa caatttcttt taaatcagct ggaattccca catcttgaga taatttttta
     3601 accgcatcaa cagcagcttt acgatatgct tcttgatcca ttcctgtagt atcaacaccc
     3661 attgcctcag caatatcttt atacttagtc cctgttgcag gagcattata ttccattact
     3721 gttggtaaaa taatcgcgtt agccactcca tgaggtgtat cgtataaagc acctaatggg
     3781 tgagccattg agtgaactat tcctaaacca acatttgaaa atcccattcc agcaatgtat
     3841 tgtcctaatg ccataccttc acgaccctca ggagtatttt caacagcacc tcttaatgaa
     3901 cgagcaataa tttcaatcgc tttaatgtgg aacatatcac tcatttccca tgctcctgcg
     3961 gtaatataac cttcgatagc atgagttaaa gcatccattc ctgtagcagc agtcaatcct
     4021 ttcggcatac ttgccatcat gtctggatca acaaatgcaa caactggtaa atcatgagga
     4081 tcaacacaaa ccatctttcg atctttttgt gcatcagtaa ttacataatt gattgtaact
     4141 tcagctgcag tcccagcggt tgtgggaaca gccaaaatag gtgtacaagg atttttagtt
     4201 tcagctacac cttcaagact tcttacatca ctgaattcag gatttttatc aatgattcca
     4261 atcgctttag cagtatccat tgaagaacct ccaccaatag cgatcatata atccgctttg
     4321 gcttcctgta aagcttttac accgctttga acattttcaa tagttggatt tggtttaata
     4381 tcagaataaa ctacatagtc taaatttgct gcatctaata agtctgtgac ttttttagta
     4441 actccaaatt taattaaatc cggatctgag cataccattg cttttttaaa gccacgagcc
     4501 tttacctcag ttacgatttc cctgatagca ccacttccat gataacttgt ttcatttaaa
     4561 ataaatctgt ttgccatatt aaaatctcct tttgttttat ttcttgataa aattttaaca
     4621 tggatttttc aatatattca agttacagat gtaagcgttt gtaacatttt cgtgattgtc
     4681 atgcaattta gttgcagtaa taaaaaagta tgtttcccta cttttttata tttaaaataa
     4741 acggctctaa ccgttgcggt aatgcttcaa tcaataattt tacaatatca tctatactaa
     4801 tcggcatatc acccaatacc cattcaacgg tcatatcaat cgatcctttg caatacatct
     4861 ttaacaaaaa atcaatatca gcagtaacaa cggtacgaac
//
LOCUS       DAOMTA010000120         4893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_416_length_4893_cov_2.364613, whole genome shotgun sequence.
ACCESSION   DAOMTA010000120 DAOMTA010000000
VERSION     DAOMTA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_4893_cov_2.364613"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLK68_04590"
     CDS             complement(<1..905)
                     /locus_tag="PLK68_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89722.1"
                     /translation="MNKKNLLLSVALSGTMLMSNGITALTAQNAVYSPGVTVEKNTNP
                     DWDADYTATFVYEDKDARDAERVTVSGNFQFYSLNDPVVKNYEKTADPTGAKVYSAYD
                     YDKDNAMFNVSGGQNNDTPIYELEETGDERFEITLPLPGNQYFYDYTVEYSDGSKVTI
                     QDPVNPSKKNSLTDHDSGHSVFYVGNSENTTAGQEYIYPRADGQTGKYEFIEYKDAHN
                     TDEETQSLGVYLPYNYDPSKTYKTIYVSHGGGGNEAEWMEIGSLPNIMDNILAEKEAA
                     ESVVVTMDNSHFGWDYDLIAKNFKE"
     gene            1379..1687
                     /locus_tag="PLK68_04595"
     CDS             1379..1687
                     /locus_tag="PLK68_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HRM89723.1"
                     /translation="MNGTCKNLLLDKRENKYIRKTYFNRNLKTTSVNQKWTTDVSDFK
                     TAMSKLYLSPILDMHSRKIVGYDISTTPSLFQTYRMLDMAFSKFFHSNQGWQYQHFSY
                     "
     gene            complement(1983..2600)
                     /locus_tag="PLK68_04600"
     CDS             complement(1983..2600)
                     /locus_tag="PLK68_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM89724.1"
                     /translation="MKIKTLLLGNMQTNGYVVSDENHHCLIIDPGANGKKVVHYLTEN
                     ELVPEAVLLTHGHFDHIGAVDYLYEHYHCPIYLHQDDLEMLDNPQLNLSVYENPFTVK
                     APVQSSHEEMKFGDFDVQWLHLPGHCPGSSMIYLKDENIIFSGDVLFKGSIGRFDFPN
                     SSKYETIESINKIKEYDFDAVIYPGHGPNSTLSEERLNNPYLKKS"
     gene            complement(2637..2786)
                     /gene="rpmG"
                     /locus_tag="PLK68_04605"
     CDS             complement(2637..2786)
                     /gene="rpmG"
                     /locus_tag="PLK68_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM89725.1"
                     /translation="MRENIIYKCTECGEENYINTKNKRNHPDRMEINKYCPRCNKKTV
                     HKEKK"
     gene            complement(2859..>4893)
                     /locus_tag="PLK68_04610"
     CDS             complement(2859..>4893)
                     /locus_tag="PLK68_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRM89726.1"
                     /translation="LQDNIVSRRLIGLLCFVIAVTSVMAIRLGIIQFKQADELAVKLE
                     QYGTTTYTSDAPRGEIVDREYVKLVENTSVICATYYAPKKIDNDVLKESAAFLAKNIN
                     IDVNDKKIISDRAKKDYFILAFKDVADGLISDKEKEEIKAQENADKRLYSLKLERITD
                     ELISQYMDEATLKYTHFLYLMQNCTSGSSILAEGLTDEEASIIGENADILPGVTVTTD
                     WARQYSNSTEFASVLGKVTTKKQGLPVELKNELLALGYQNDSRVGTSGLEEQYEDILR
                     GNDSTYTLNYDSSGNPIITSTKAGTAGSNIRISIDWELQQLANQKIEEELKACNSSNK
                     YFNKMFFILMDPYTGEIIVMAGKTIDKATGEVSDYASGNYLDANKIGSTIKGGSIYTG
                     FKEGVIGPNTYFVDEPIKIKGTKAKKSWKSFGTINEVDALAYSSNVYMFRIAMLLGGA
                     NYVYDGPLKINEEAFDTLRNDLGELGLGVKTGLDVSNEALGYRGKQRTGGLLLDAMIG
                     QYDTYTNIQLAQYACTLANGGKRIQPHLLLDSYTTDEDGEIQVNYEANTNVLDDVSNQ
                     ATAFSQIKQGMRACVTRTEGTAHSWNSKPYVTYAKTGTAEDYTTDDGKTGTTDYPNHL
                     QIGYVQTTEDSRPEVAFACMSYRQTTATSGSSSAPIVAQAVIDRYWEKYHSTN"
BASE COUNT         1543 a          927 c          673 g         1750 t
ORIGIN      
        1 ttttctttga aatttttcgc aattaaatca taatcccatc caaagtgact gttatccatt
       61 gttacaacaa cactttctgc tgcttctttt tccgcaagga tattatccat aatatttggc
      121 aatgaaccaa tttccatcca ttcggcttca tttccgccac cgccatgtga tacataaatc
      181 gttttatatg tttttgaagg atcatagttg tatggtagat aaacacctaa tgactgtgtt
      241 tcttcatctg tgttatgtgc atccttgtat tcaataaatt catatttccc tgtttgaccg
      301 tcagctcttg gataaatata ctcctgtcct gctgttgtat tttcactgtt tccaacatag
      361 aaaacactgt gtcccgaatc atgatcagtt aagctgtttt tctttgatgg atttaccgga
      421 tcctgaatcg taacttttga accgtcacta tactctactg tataatcata aaaatactga
      481 ttgcccggta acggcagtgt aatctcgaat ctttcgtctc ctgtttcctc cagttcataa
      541 atgggtgtat cgttattttg tccgccgctt acattgaaca ttgcgttatc cttatcatag
      601 tcataagcac tgtaaacctt ggctccggta ggatcagctg ttttttcata atttttaaca
      661 accggatcat ttaatgagta aaactgaaaa ttacctgaaa ctgttactct ttctgcgtca
      721 cgtgcatctt tgtcttcata aacaaaggtt gcggtataat cggcatccca gtctggatta
      781 gtatttttct ctaccgttac gcccggacta tatacagcat tttgagccgt taatgcggta
      841 ataccattgg acattagcat agttcctgat aatgccactg acaataacaa attcttttta
      901 ttcatattat cccttccttt cactaacatt ctatccgaag aaggaataag aacgccacat
      961 tcctttatgc aagctgcttt cattttttgc aagcaggttt tataatttga aagagcatgc
     1021 gggcagaaaa gttaatatat tcattaatta tataagctag tagaatatca agttgcaata
     1081 tatttacact aagataatta tatcatgact cattttttat tatttcaacc atgagattct
     1141 ttcctaaaat aaactcacag cgatcaacac gcattattgt aaaatactac aataattgaa
     1201 ttattaaaaa aatcgtaaaa aattatagcg aaccgaataa gttgtccaac ctattcggtt
     1261 cactttttat atgtccaaac ttacgaataa acaaagaata gtggtactat gtcacatatg
     1321 ggtttatatg cacttaatcc tactcctaag caacgttata actcttataa ggggggaaat
     1381 gaatggaact tgtaagaact tacttcttga taaacgagag aataagtata ttagaaaaac
     1441 atattttaat agaaacctta aaactacatc ggttaatcaa aaatggacaa ctgatgtttc
     1501 tgattttaaa acagctatga gtaaattata tttatctcca attttagata tgcatagtag
     1561 aaaaatcgta ggatacgata tatcaactac accaagtcta tttcaaacat accgaatgtt
     1621 agatatggct ttctctaaat ttttccatag taatcaagga tggcagtacc aacatttctc
     1681 atattaaaat aaattaaaag aaaaaggaat tacttaatca aagtctcgaa agggaacggt
     1741 caggataatt tactaataga aaacttcttg gaaaaaatga agaatgaaat gttttatgga
     1801 tttgaagata cattcaaatc attagaagag ttaaaacaag ctatgataga ttatatcgaa
     1861 taccataata attctataat tacagtaaaa agaaaaggct taacccctat acaaattagg
     1921 aatcaagcct tacagttaat ttaaattatt ttttgtccaa caaaggcagt tcactataaa
     1981 aattacgact tttttaaata tggattattt aggcgttctt cacttaacgt agagtttggt
     2041 ccatgaccag gataaataac agcatcaaaa tcatattcct ttattttatt tatgctttcg
     2101 attgtttcat actttgatga atttggaaaa tcaaagcgtc caattgaccc tttaaacaat
     2161 acatctccag aaaaaattat attttcatct tttaaataaa tcattgacga tcctggacaa
     2221 tgtcctggta aatgtagcca ttgtacatca aaatccccaa acttcatctc ctcatggctt
     2281 gactgaactg gtgctttaac agtaaaagga ttttcataca ctgataaatt taactgagga
     2341 ttatctagca tttctaaatc gtcttgatga agatagatag gacagtggta atgttcgtat
     2401 aaatagtcaa ccgcaccaat atgatcgaaa tgtccatgag ttaataaaac agcttctgga
     2461 actagttcat tttccgtcaa ataatgcaca actttcttac cattagctcc aggatcaatg
     2521 attaagcaat gatgattctc atcacttacc acatagccat tagtttgcat attacctagc
     2581 agtaatgttt ttattttcat cttcttcctc ctataaagaa aaaaagccaa taaggcttat
     2641 ttcttttctt tatgtacagt ttttttatta catcttggac agtatttgtt gatttccatt
     2701 ctatctggat gatttctttt attttttgtg ttgatatagt tttcttcacc acattcagta
     2761 catttataaa tgatattttc tctcattagt tacacctcca ttagtaaact tccgtctaat
     2821 atattatcat agtatttaaa taattactag cacttttttt aatttgttga gtgatacttc
     2881 tcccagtaac gatcaattac tgcttgagca acgatcggag cagaactaga tccgcttgtt
     2941 gcagtagttt gtcgataact catacaagca aaagcaactt ctggtctaga atcctccgtt
     3001 gtttgcacat aacctatttg taaatgatta ggataatcgg ttgttccagt cttaccatca
     3061 tcagttgtat aatcttctgc tgttccggtt ttagcataag ttacatatgg ttttgaattc
     3121 caagaatgtg cagttccttc agttcgggta acacaagctc tcattccttg tttaatttgg
     3181 ctaaacgccg tcgcttgatt actaacatca tctaatacat ttgtatttgc ctcataatta
     3241 acttgtattt ctccatcctc atcagtagta tatgagtcta aaagtaagtg gggctggatt
     3301 cttttcccgc cattagccaa tgtacatgcg tattgtgcta attgaatatt tgtgtaagta
     3361 tcatactgac caatcattgc atctaataac aacccacctg tacgttgttt tcctcgatat
     3421 cctaatgctt catttgaaac atctaatcct gttttaactc ctaaccctaa ctcacctaaa
     3481 tcatttctta aagtatcaaa agcttcttca tttattttca acggcccatc ataaacataa
     3541 tttgccccac ctaaaagcat tgctatccta aacatataaa catttgatga ataagccaga
     3601 gcatcaactt cattaatagt tccaaaagat ttccaagact ttttagcttt tgttccttta
     3661 attttgattg gctcatcaac aaaataagtg tttggtccaa ttactccttc tttgaaacca
     3721 gtataaattg aacctccctt aattgttgaa ccgattttat ttgcatctag gtaattccct
     3781 gaagcataat cactaacttc accagttgct ttatcgattg ttttgccggc cattacaata
     3841 atttcacccg tatatggatc cattagaata aaaaacattt tattaaaata tttattactg
     3901 ctattacaag cttttaattc ctcttcgatt ttttgattag ccaattgttg aagttcccaa
     3961 tcaattgaaa tacgaatatt tgaacctgca gttcctgctt tagtacttgt tattattggg
     4021 tttcctgagc tatcataatt taaggtatag gtactatcat tccctcgtaa aatatcttca
     4081 tactgctcct ctaagcccga agttcctact ctagaatcat tttggtatcc caatgctaat
     4141 aattcatttt ttaattctac aggtaaccct tgttttttag tcgtcacttt gcctaaaact
     4201 gaagcaaact ctgttgaatt tgaatattga cgtgcccaat ctgttgtaac tgtcaccccg
     4261 ggtaagatat ctgcattttc accaataata ctagcctcct catcagttaa accttcagcc
     4321 aaaattgacg aaccactcgt acaattttgc atcaagtata aaaagtgagt atattttaat
     4381 gttgcttcat ccatatattg actaattaat tcatcagtaa ttctctctaa tttaagacta
     4441 tataatcttt tgtctgcatt ttcttgagct ttgatttctt ctttttcttt atcgctaatc
     4501 aatccgtcag caacatcttt aaaagctaaa ataaaataat ccttttttgc tcgatcagaa
     4561 atgatttttt tatcatttac atcaatattg atattttttg ctaaaaaagc agctgattct
     4621 tttaaaacat cattatcaat ttttttaggt gcataatatg ttgcacaaat tacacttgta
     4681 ttttccacta attttacata ttcacggtca acaatttctc ctctaggagc atcactcgta
     4741 tatgtagtag taccatattg ttccagcttt actgccaatt catcagcctg tttaaattgt
     4801 ataattccta aacgtattgc cattacggaa gtaacagcaa taacaaaaca aagtagtccg
     4861 attaatcgcc gactaacaat gttatcttgt aaa
//
LOCUS       DAOMTA010000121         4866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_421_length_4866_cov_2.381833, whole genome shotgun sequence.
ACCESSION   DAOMTA010000121 DAOMTA010000000
VERSION     DAOMTA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_4866_cov_2.381833"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLK68_04615"
     CDS             <1..216
                     /locus_tag="PLK68_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM89727.1"
                     /translation="NIIIRCIKRICQEKRNKKVSIYCGIIMMIVTAIYLGCSFTTNAW
                     HISWIVYPIGGIICGIVWLLFEAHEED"
     gene            327..>901
                     /locus_tag="PLK68_04620"
     CDS             327..>901
                     /locus_tag="PLK68_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89728.1"
                     /translation="MSLGKRIQSYRKQKGLSQEQLASRLNISRQALSKWESDINVPNI
                     DKIMDVAKALEITLNELLGLEEDSNDEYAKLESILNQVVLTQNNEIIRNKKYLKMGLV
                     IGTGIFIILALVMWSVLNDIKGIKSTIGNNNAGLSNQINIINNQLSSLENSLFDQLQE
                     QLQAQGSLLSSFELQIEDIDFKTQTMKVKIDL"
     assembly_gap    902..911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <912..1483
                     /locus_tag="PLK68_04625"
     CDS             <912..1483
                     /locus_tag="PLK68_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRM89729.1"
                     /translation="HYEDTSSVVVVLDYEHYDDRSYLLTLSDGHFKTSEDLPIDNILN
                     AAVTIGTDSKQAQLLKNVADYILPELKLNYGEVYEIIYQQDSLNITLNPDDTVNLNES
                     GILSKYKKDNKLVKFDGFYYLNDQKHTVKVTTDKQRNILFSIPANDFKNGDKLKIVIN
                     LVDKTGITSQIEKNFIVAIIDDSVSLYEE"
     gene            1649..2314
                     /locus_tag="PLK68_04630"
     CDS             1649..2314
                     /locus_tag="PLK68_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRM89730.1"
                     /translation="MYKYLEPYAAMIEFLGKALGDNVEIALHDLTSKEQEIVAIANNH
                     NSGREVGAKLSNLSLHYLEEKQYLDNDYVMNYKTTGPDGKLMRSATYFIKEPGKEMPV
                     GMLCINVNISDLEYLTSTIKKILGIKDEKDIEFKMDNPVEILSSPLDEMIDLYIKECL
                     EKMGFPSYFLAERLNVDEKIKVVKYLQEKGTFKVKGAIVLVAEKLAVSEPTVYRYLKK
                     MEK"
     gene            2324..3964
                     /locus_tag="PLK68_04635"
     CDS             2324..3964
                     /locus_tag="PLK68_04635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRM89731.1"
                     /translation="MKELVYVSGHKNPDTDSICSAIGYAYLLKAIDRYNAVPVRLGEV
                     SRETEYVLKRFNLEIPTLLKTVKQKVEDLNYDKVTVFSKELTLKTAWSLMKQQNLKSA
                     PILDDHGQLLGLLSTSNIVEGYMEKWDSSLLKDAHTPIENVIDTLEASVIYLNHNLKT
                     IEGDLHIAAMRGEEASKRVRPGDIVIIGGDRDDAVNSMIDAEVSLIILTGSLGVEAEV
                     LNKLKEKGISVISTSFNTYLTSQQIIQAIPVEYIMQKGDLKLFSTDDTLDHVKEVMSE
                     TRYRSYPVLDLNNRCVGSISRFALLKGLRKKVILVDHNERGQSIPGIEEADILEIVDH
                     HRVADIQTIGPLLFRGEPLGSTATIVTKIFDENDVEIPQSIAGALLGAIISDTLLFKS
                     PTCTPVDTKAARKLAKIAGVDIEEFAMEMFKAGTSLVGKTVEEIFNQDFKKFPFEQGT
                     VGVGQVNSMDIEGFMPYKADMLDYMDKFAEDNRLDFTLLLLTDIINANSEIFVAGPKP
                     YLVEEAFNIKLNDHQATLKGVISRKKQVVPAITAVMSK"
     gene            3966..4235
                     /locus_tag="PLK68_04640"
     CDS             3966..4235
                     /locus_tag="PLK68_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM89732.1"
                     /translation="MRLDKFLKVSRIIKRRTLSKEISESSRVKVNGKVAKPSTKLKVG
                     DEIEIEFGRSRLTVKVKELREHVLKEDSTMLYEIINEERIERDLN"
     gene            4490..>4866
                     /locus_tag="PLK68_04645"
     CDS             4490..>4866
                     /locus_tag="PLK68_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-biosidase"
                     /protein_id="HRM89733.1"
                     /translation="MKGLKKLFSAMLVLTMLFGTIANVGMAKIYAAEEGMKRVFSIDA
                     GRKYFSEEQLLQIIDKAYLNGYTDVQILLGNDALRFFLDDMSITVDGKTYASEAVKKA
                     ITAGNDHYYKDPNGNALNETEMNR"
BASE COUNT         1802 a          547 c          921 g         1586 t
ORIGIN      
        1 aacattatta ttagatgcat taaaagaata tgccaagaaa aaaggaataa aaaagtctca
       61 atatattgtg gtatcattat gatgattgtt acagcaattt atcttggctg ctcatttact
      121 acaaacgcct ggcatatttc atggattgtt tatcctatcg ggggaattat ttgtggcatt
      181 gtttggctcc tttttgaagc acatgaagaa gattagaaaa atgctaatct tttttgtttt
      241 gcgcggataa atagcaaaat gtaaattatt gtatgtagtt atattaagag ggtcatgtta
      301 agattaaagg gtaggagggg ataattatga gcttaggaaa acggattcag tcgtatcgaa
      361 aacaaaaagg attatcacaa gagcagctgg catcaagatt aaatatttcc agacaagccc
      421 tatcaaaatg ggaatctgat ataaatgtcc ccaatattga taagattatg gatgtagcaa
      481 aagctctaga aatcacgctt aatgaattac ttggtttaga agaagattca aatgatgagt
      541 atgctaagtt ggaaagtatt ttaaatcaag tggtgttaac tcaaaataat gagattataa
      601 gaaacaagaa atatttaaaa atgggtttgg taattgggac gggaatattt ataattcttg
      661 ctcttgttat gtggagtgtt ttaaatgata ttaaaggaat taaaagcacg attggaaata
      721 ataatgcagg tttaagtaat cagatcaata taatcaataa ccaattatca tcattagaaa
      781 attctctttt tgatcagctt caagaacaat tacaagccca aggttcttta ctaagctctt
      841 ttgagcttca aattgaggat attgatttta aaacacagac aatgaaagtc aaaattgatc
      901 tnnnnnnnnn nagcattatg aggatactag tagtgtggta gttgttttag attatgagca
      961 ttatgatgat cggtcatatc tattaacatt aagtgatgga cattttaaga caagtgaaga
     1021 tttacctatt gataatattt taaatgctgc cgtgacaatt ggaacagata gtaaacaagc
     1081 acagttatta aaaaatgttg cagattatat attgcctgaa ttgaaactta attatggtga
     1141 agtttatgaa ataatctatc agcaggattc gttaaacatt acgttaaacc cagatgatac
     1201 tgtaaatttg aatgagagtg gtatattgtc aaaatataaa aaagataata aattagtaaa
     1261 attcgatggg ttttattatt taaatgatca gaaacatact gttaaagtta caacagataa
     1321 gcagagaaat attctttttt caattcctgc taatgacttt aagaatggtg ataaactaaa
     1381 aatagtaatt aatttggtcg ataaaacggg gatcacttct caaattgaaa agaattttat
     1441 tgtagcaatt atagatgatt cagttagtct gtatgaagaa taaagcacta gtattatatt
     1501 agtgcttttt atcatgataa aactaaataa catttagata ttatagtgat aataaagtta
     1561 gaaatttcta aaacaattat tatcatgata aaaaatactg ttattttgtt tagaattcgt
     1621 gtataattgc catgaaggag tgataactat gtataaatat ttggaaccat atgcagcaat
     1681 gatagaattt ttagggaagg ctttaggcga taacgttgaa attgcgttac atgatttaac
     1741 ttcaaaagag caagaaatcg ttgcaattgc taataaccat aactcaggaa gagaagttgg
     1801 ggctaaatta tcaaatttat cattacatta tttagaagaa aaacagtatt tagataatga
     1861 ttatgtaatg aattacaaga caactggacc agatggaaaa ttaatgcgtt cagcaactta
     1921 ttttattaaa gagcctggta aggaaatgcc agtaggtatg ctttgtatca atgtaaatat
     1981 ttctgattta gaatatttaa catcgacgat taaaaaaatc ttaggtatta aagatgaaaa
     2041 agatattgag tttaagatgg ataatccagt tgaaatttta tcttcaccgt tagatgaaat
     2101 gattgatctt tatattaaag aatgtttaga gaaaatgggt ttcccaagtt atttcttagc
     2161 agaacgtttg aatgttgatg aaaagatcaa ggttgttaaa tacttacaag aaaaaggtac
     2221 ttttaaagta aaaggagcta ttgttttagt agctgaaaaa ttggcagttt ctgaacctac
     2281 agtatatcgt tatttaaaaa agatggaaaa ataggagagt aaaatgaaag aattagtata
     2341 tgtaagtggt cataaaaacc ctgataccga ttcaatctgt tcggcaattg gatatgctta
     2401 tttattaaaa gcaattgacc gttataatgc cgtaccagta agattgggtg aggtaagtcg
     2461 ggagactgag tatgttttaa aaagatttaa tttagaaata ccaactttat taaaaactgt
     2521 taagcaaaaa gtagaagatt taaactatga taaagtaact gtattttcaa aagaattaac
     2581 attaaagact gcatggtctt taatgaaaca acagaactta aaatcagcac caatcttaga
     2641 tgatcatggg caattattag gattattatc aacttctaat attgttgaag ggtatatgga
     2701 aaaatgggat agtagtttat taaaagatgc gcatacacca attgaaaatg taattgatac
     2761 attagaggca tcagtaattt atttaaacca taatttaaaa acaatcgaag gtgatcttca
     2821 tattgcagca atgcgtggtg aagaagctag taaacgtgtt agaccaggag atattgtaat
     2881 tattggtggt gatcgtgatg atgctgttaa ttcaatgatt gatgctgagg tttcattgat
     2941 tattttaact gggtctttgg gtgttgaagc tgaagtacta aataaattaa aagaaaaggg
     3001 aatcagtgtt atttcaactt cttttaatac atatttaact tcacaacaaa ttattcaggc
     3061 aattccggta gaatatatca tgcaaaaggg agatttaaaa ttattctcaa cagatgatac
     3121 attagatcat gtaaaagagg tcatgagtga aacaagatat cgtagttatc cagttcttga
     3181 tctaaataat cgttgcgttg gaagtatttc tcgttttgct ttattaaaag gattaagaaa
     3241 gaaagttatt ttagttgatc ataatgagcg tggtcaatca attcctggaa ttgaagaagc
     3301 tgatatttta gaaattgttg accatcatcg tgttgccgat attcaaacga tcggaccatt
     3361 attatttagg ggtgaaccat taggatcaac ggcaacgatc gtaacaaaga tttttgatga
     3421 aaatgatgta gaaattcctc aatctattgc tggggcttta ttaggagcaa ttatttcaga
     3481 tacattatta ttcaaatcac caacatgtac acctgtagac actaaggctg ctcgtaaatt
     3541 agctaagatc gctggggttg atattgaaga atttgctatg gagatgttta aagcaggaac
     3601 ttcattagtt ggtaaaacag ttgaagaaat ctttaatcaa gatttcaaaa aattcccatt
     3661 tgaacaagga actgttggtg ttggacaagt taattcaatg gatattgaag gatttatgcc
     3721 atataaagca gatatgcttg actatatgga caaatttgca gaagacaacc gtttagattt
     3781 tactttatta ttattaacag atattattaa tgctaatagt gaaatctttg ttgctgggcc
     3841 taaaccatat ttggttgaag aagcatttaa tattaaatta aatgatcatc aagcgacatt
     3901 aaaaggggta atttctcgaa aaaaacaagt tgttccagca atcacagcag taatgagtaa
     3961 ataagatgag attagataaa tttttgaaag tatcgagaat tattaagcgt cgtactttat
     4021 caaaggaaat tagtgaaagt tcacgggtaa aagttaatgg taaagtcgct aaaccaagca
     4081 caaaacttaa agttggtgat gaaatcgaaa ttgaatttgg acgtagtcgt ctgactgtta
     4141 aggttaaaga acttcgcgaa catgtcttaa aagaagatag tacgatgtta tatgaaataa
     4201 ttaatgaaga acgaattgag cgagatttaa attagaaacc agtattggtt tcttttttat
     4261 aaaatgatat atttttatta tattggttga cttttactca ttagaaaagt tatcataata
     4321 tatggcgata ggattgttac tattagatta ggcaagacca aattggagaa atacgaataa
     4381 cgtgtagcta tatgcagttg ttttcgtata aatttcaatt tggtctttta tattttattt
     4441 aataggatta attatattat cgctgttcta tacggaaaag gaggataaga tgaaaggatt
     4501 aaaaaaatta ttttcagcta tgctggtgct gacaatgctt tttgggacga ttgcaaatgt
     4561 tggtatggca aagatttatg cagctgaaga aggaatgaag cgggtgtttt ctattgatgc
     4621 cgggagaaaa tacttttcag aagaacagct tttacaaatt attgacaaag cttatttaaa
     4681 tggttacaca gatgttcaaa ttttgttagg gaatgatgca ttacgttttt tcctagatga
     4741 tatgtcaatt actgttgatg gtaagacata tgcaagtgaa gcggtgaaaa aagcaattac
     4801 agcaggaaat gatcattatt ataaagatcc aaacggtaat gctttaaatg agactgaaat
     4861 gaatcg
//
LOCUS       DAOMTA010000122         4856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_423_length_4856_cov_2.138096, whole genome shotgun sequence.
ACCESSION   DAOMTA010000122 DAOMTA010000000
VERSION     DAOMTA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_4856_cov_2.138096"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLK68_04650"
     CDS             complement(<1..726)
                     /locus_tag="PLK68_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRM89734.1"
                     /translation="MADKRDYYEVLGVSKQASADEIKRAYRKKAKQYHPDVNKEPGAE
                     EKFKEVQEAYEVLSDANKKATYDRFGHAAFEQGAGNGGAGGFGGFGFEDVDLGDIFGS
                     FFGGGGRSRQRNTGPRRGDDRLMRLNISFMEAVNGVKKDIKVTYDAPCTHCNGSGAKN
                     PNDVQTCSRCGGRGTVQQQQSSPFGTFVTETTCPDCNGTGKVVKEKCPHCHGRGYETK
                     TVTVQLDIPAGINSNQQLRVAGKG"
     gene            complement(787..2604)
                     /gene="dnaK"
                     /locus_tag="PLK68_04655"
     CDS             complement(787..2604)
                     /gene="dnaK"
                     /locus_tag="PLK68_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRM89735.1"
                     /translation="MGKIIGIDLGTTNSCVSVMENGEVKVIANPEGNRTTPSVVSFKN
                     GEIIVGEAAKRQAVTNPDTIMSVKRHMGTDHKEHANGKDYTPQEISAMILQNLKATAE
                     AYLGETVTQAVITVPAYFNDAQRQATKDAGKIAGLEVERIINEPTAAALAFGLDKLDQ
                     EQKVLVYDLGGGTFDVSILDLADGTFEVLATAGDNKLGGDDFDQVIINWLVQEFKNNE
                     GADLSKDPMAMQRVKEAAEKAKKDLSGTMQTQISLPFISAGAAGPLHLELTLTRAKFD
                     ELTRDLVLRTETPVRQALKDAGMDPTEIHQVLLVGGSTRIPAVQESVKKLLGKDPNKS
                     VNPDEVVSMGAAIQGGVIAGDVKDVLLLDVTPLSLGIETMGGVMTVLIERNTTIPTTK
                     SQVFSTAADNQPAVDINVLQGERSMAKDNKQLGLFKLDGIEPAPRGVPQIEVTFSIDV
                     NGIVNVKAKDLKSQKEQSIVIQNSTGLSDEEIDRMVKEAEANKAEDEQKRKDIETRNK
                     AEQMINEIDKALAEQGDKIDATQKESAEKLKDELKAALDNNDMATLEAKMSELEQMAQ
                     QMASYAYQQQGGAGASDANAGTANAQDDNVVDADFEEKN"
     gene            complement(2622..3173)
                     /gene="grpE"
                     /locus_tag="PLK68_04660"
     CDS             complement(2622..3173)
                     /gene="grpE"
                     /locus_tag="PLK68_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRM89736.1"
                     /translation="MNVVAKEKETVVDEESTEKTAEETVETKEDEITVEDQLKNLEDE
                     VNTWKTDYYKVFADMENLKKRLQNEHANAMKFMMQSFIEELLPVVDNFERSLAVVDPS
                     DEIKNFLKGYEMIYNQLMEVLKSQGVEVIKTEGEEFDPNFHQAVMTVKDDNFKTNMIV
                     EELQKGYKLKDRVIRASLVKVSE"
     gene            complement(3176..4216)
                     /gene="hrcA"
                     /locus_tag="PLK68_04665"
     CDS             complement(3176..4216)
                     /gene="hrcA"
                     /locus_tag="PLK68_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRM89737.1"
                     /translation="MLTARQLLVFKCIVDEFIETAEPVGSKALMTKYQLPYSSATIRN
                     EMSFLEEYGYLEKTHTSSGRVPSVEGYRFYVDNLAKRDLDEGIKNQVAQIFSDRHRGL
                     NEIIHESCEMLSELTHLTSVVLGPDSSEDTLQQINIVPLNDNRVTAIIITNQGYVENK
                     VFDLNRNHKIDDLVSCVAVMNDLLIGTPIDQVAFRLERDVKPVLSAKIKEHEELFNAF
                     LEAFVRFASSNVYFSGKENMLYQPEYNDVNKLRRIVSAFENSQVWNALQPLSDEEGVT
                     VRIGSDSPINEIEDVSVISASFRTGERTKGSISVIGPTRMPYEKVVSLVEYISRNIEE
                     AFFDESDDSNDE"
     gene            complement(4402..>4856)
                     /locus_tag="PLK68_04670"
     CDS             complement(4402..>4856)
                     /locus_tag="PLK68_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRM89738.1"
                     /translation="NSCVYNVGVLLDDIDMDVIRRFGFQSGKDVNKFDGIDYEVDCQN
                     IKQITEGIAASFSVKVISMTDVGTHIMFVGDVIDCKVINEGEVLTYANYHNKKNGTTP
                     KNASSYQADTSKHGWRCTVCGFILEADELPEDFICPVCKQPASVFEKI"
BASE COUNT         1458 a         1031 c          632 g         1735 t
ORIGIN      
        1 acctttgcca gctacgcgca attgttgatt agaattaata ccagctggta tatctaattg
       61 tactgtcact gtcttagttt catatccacg tccatgacaa tgtggacatt tctctttaac
      121 aacttttcca gttccattac agtcaggaca agtagtttca gttacaaatg tcccaaatgg
      181 tgaactttgc tgctgttgaa cagttccacg tccaccacag cgtgaacacg tttggacatc
      241 attaggattt ttagccccac taccgttaca atgagtacat ggtgcatcat aagtaacttt
      301 aatatctttc tttacaccat ttactgcttc catgaaactg atatttaaac gcattaagcg
      361 atcatcacca cgacgtggtc ctgtatttct ttgacgtgat cttccgccac caccaaagaa
      421 agatccaaaa atatctccta aatcaacatc ttcaaatcca aatccaccaa aaccaccagc
      481 accaccatta ccagcacctt gctcaaatgc agcatgacca aagcggtcat aagtggcttt
      541 tttattagca tctgataata cttcataagc ttcttggact tctttgaact tttcttcagc
      601 accaggctct ttgttgacat caggatgata ttgttttgct tttttacgat aagctcgttt
      661 gatctcatca gctgaagcct gtttacttac acctaagact tcataataat ctcttttatc
      721 agccatattt tcaccctctt tttatactat ttaaaaccaa ggcggcaaag ccttggcttt
      781 aaatgattag ttcttttctt cgaaatctgc atcaacaaca ttatcatctt gtgcatttgc
      841 tgtaccagca tttgcatcac tagctcctgc tccaccttgt tgttgatatg catatgaagc
      901 catttgttgt gccatttgtt ctaattcaga cattttagct tctaatgtag ccatatcgtt
      961 gttatctaaa gctgccttta actcatcttt taatttttca gcagattctt tttgagtagc
     1021 atcaattttg tcaccttgtt ctgctaaagc tttatcgatt tcattaatca tttgttcagc
     1081 tttatttctt gtttcgatat ctttacgttt ttgttcatct tcagctttat tagcttcagc
     1141 ttctttaacc attctatcaa tttcttcatc acttaaacca gttgagtttt gaataacgat
     1201 agattgttct ttttgagatt ttaaatcttt agctttaaca tttacgatac cattaacgtc
     1261 aatgctgaaa gttacttcga tttgaggtac accacgtgga gctggttcaa taccgtctaa
     1321 tttaaataaa cctaattgtt tattatcttt agccattgat ctttcacctt gtaaaacgtt
     1381 gatatctaca gcaggttgat tatcagcagc agttgagaat acttgagatt tagttgttgg
     1441 aatagttgta tttctttcaa ttaatactgt cattacacca cccatagtct caatacctaa
     1501 tgataatgga gtaacatcta gtaataatac atctttaaca tcaccagcaa taaccccacc
     1561 ttgaattgca gcacccatag atactacttc atcagggtta acagatttat ttggatcttt
     1621 tcctaataat tttttaactg attcttgaac agcaggaata cgagtagaac ctcctactaa
     1681 taatacttga tgaatttcag ttgggtccat accagcatct ttcaatgctt gacgtacagg
     1741 agtttcagtt cttaaaacta aatcacgagt taattcatca aattttgctc tagttaatgt
     1801 taattctaag tgtaaaggcc ccgctgcacc agctgagatg aatggtaatg aaatttgtgt
     1861 ttgcatagtt cctgataagt cttttttagc tttttcagca gcttctttaa cacgttgcat
     1921 tgccattgga tctttcgata aatcggctcc ttcattattt ttgaattcct gaaccaacca
     1981 attgattata acctgatcaa aatcatcacc accaagttta ttatccccag cagtagctaa
     2041 tacttcaaaa gttccatcag ctaagtctaa gatagaaacg tcaaatgtac ctccacctaa
     2101 gtcatatact aatacttttt gttcttgatc taatttatct aatccaaacg ctaaagcagc
     2161 agcagttggt tcattgataa tacgttctac ttctaatcca gcaattttac cagcatcttt
     2221 agtagcttga cgttgtgcat cgttaaagta tgcaggaact gtaataactg cttgtgttac
     2281 agtttcacct aaatatgctt cagctgttgc ttttaagttt tgtaagatca ttgctgaaat
     2341 ttcttgtggt gtatagtctt taccatttgc atgttcttta tgatcagtac ccatatgacg
     2401 tttaactgac ataattgtat ctggattagt tactgcttga cgtttagcag cttcaccaac
     2461 gataatctct ccatttttaa atgatactac tgaaggagtt gttctgtttc cttctggatt
     2521 agcgattact ttaacttcac cgttttccat aacactgaca catgaatttg ttgtacctaa
     2581 gtcaatacca ataattttac ccataattaa attcctccta tttattcact tacttttact
     2641 aaagatgcac gaataacgcg atcttttaac ttatatcctt tttgtaactc ttcaacaatc
     2701 atatttgttt taaagttatc atcttttacg gtcataactg cttgatggaa gtttggatca
     2761 aactcttcac cctcagtttt aattacttca acaccttgtg actttaatac ttccatcagt
     2821 tggttataaa tcatttcata acccttcaaa aagtttttaa tttcatcact aggatcaact
     2881 acagctaacg atctttcaaa attatctaca acaggtaata attcttcaat aaatgattgc
     2941 atcataaatt tcattgcatt agcatgttcg ttctgtaatc tcttcttaag attttccata
     3001 tcagcaaaca ctttataata atcagttttc caagtattta cttcatcttc taaattctta
     3061 agttgatctt caactgtaat ttcatcttct tttgtttcaa ctgtttcttc tgcagtcttt
     3121 tctgtagatt cttcatctac tactgtttct ttttccttag ccactacatt cacctctatt
     3181 catcgtttga atcatctgat tcgtcaaaga acgcctcttc aatattcctt gaaatatact
     3241 ctactaagga aactactttt tcataaggca ttcttgttgg cccaattact gaaatagatc
     3301 ccttggttct ctctcctgtt ctaaatgaag ctgagatgac cgatacatcc tcaatttcat
     3361 taatcggtga atcagaacca attcttaccg ttactccttc ttcatcagat aaaggttgta
     3421 aggcattcca tacttgagag ttttcaaatg cactaacgat tcttctcaat ttatttacat
     3481 cattatactc aggctggtat aacatatttt cttttcctga aaaatataca ttgctcgaag
     3541 caaatcgaac aaaagcttca agaaaagcat taaataattc ctcatgctcc ttgattttag
     3601 ctgataacac cggttttaca tcacgttcta aacgaaatgc tacctgatca atcggtgtcc
     3661 ctatcaataa atcattcatc actgcaacac aactcaccaa atcatcaatt ttatgattac
     3721 ggtttaaatc aaacaccttg ttttcaacat agccttgatt cgtaataata atcgcagtaa
     3781 ctcggttatc attcaaagga acaatattga tttgctgcaa agtatcttcg ctagaatctg
     3841 gtcccaagac taccgatgtt agatgcgtta gttcacttaa catctcacaa ctttcatgaa
     3901 taatttcatt caaaccacga tgacgatcgc taaaaatttg cgctacttgg tttttaatcc
     3961 cttcatctag atcccgcttt gcaagattat caacatagaa tcgatatcct tcaaccgaag
     4021 gaactcgtcc ggaagatgta tgagtttttt ctaaataacc atactcttca agaaaactca
     4081 tctcattacg aatggttgca cttgaataag gcaattgata ttttgtcatc aaagcttttg
     4141 acccaactgg ttcagccgtt tcaataaact catcaacaat acatttaaat actaatagtt
     4201 gcctcgctgt aagcatcagt ttcaccaccc ttagcactca tctttccttt gtgctaaata
     4261 cattatacat caataattag cactgtcaac acctttgtgc taaaaaataa aaaaagaatt
     4321 ataaatccga tttctattta aaacaactct ataatcaatc taattccttg ataaaatcac
     4381 aaaaaaacag ccctaaggct gttaaatttt ttcaaaaaca cttgccggtt gtttacaaac
     4441 tggacaaata aaatcttcag gcaattcatc tgcttctaaa ataaatccac aaacagtaca
     4501 tctccaacca tgttttgaag tatctgcttg ataactagaa gcatttttag gtgttgttcc
     4561 atttttctta ttatgataat ttgcataggt taatacttca ccttcattaa tcactttaca
     4621 atcaataaca tcgccaacaa acataatatg tgttccaaca tctgtcattg aaataacttt
     4681 aacactaaat gaagcagcta ttccttcggt gatttgttta atattttgac aatcaacttc
     4741 ataatcaatc ccatcaaatt tgttaacatc tttaccagac tgaaaaccaa atcttctgat
     4801 aacatccata tcaatatcat caagtaatac cccaacgtta taaacacaac tatttt
//
LOCUS       DAOMTA010000123         4853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_426_length_4853_cov_2.513547, whole genome shotgun sequence.
ACCESSION   DAOMTA010000123 DAOMTA010000000
VERSION     DAOMTA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_426_length_4853_cov_2.513547"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..791
                     /locus_tag="PLK68_04675"
     CDS             180..791
                     /locus_tag="PLK68_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM89739.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVEN
                     YSGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVAD
                     LPTTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIRQGLTRSGYKPTG
                     KKANEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAQVI"
     assembly_gap    964..973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1018..1902)
                     /locus_tag="PLK68_04680"
     CDS             complement(1018..1902)
                     /locus_tag="PLK68_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HRM89740.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
     gene            complement(1958..3433)
                     /gene="msr(E)"
                     /locus_tag="PLK68_04685"
     CDS             complement(1958..3433)
                     /gene="msr(E)"
                     /locus_tag="PLK68_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HRM89741.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            complement(3926..4198)
                     /gene="nadS"
                     /locus_tag="PLK68_04690"
     CDS             complement(3926..4198)
                     /gene="nadS"
                     /locus_tag="PLK68_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HRM89742.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKT
                     GLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
     gene            complement(4191..4511)
                     /locus_tag="PLK68_04695"
     CDS             complement(4191..4511)
                     /locus_tag="PLK68_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM89743.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGG
                     IRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILHQLVKE
                     QFHG"
     gene            4651..>4853
                     /locus_tag="PLK68_04700"
     CDS             4651..>4853
                     /locus_tag="PLK68_04700"
                     /inference="COORDINATES: protein motif:HMM:NF025918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HRM89744.1"
                     /translation="MQNKLDNIIQELKELSGNSRLNIELPDDIFISAYERKIGFIFPK
                     DYKKVLKEISNIFYGTIELASLT"
BASE COUNT         1499 a          953 c          832 g         1559 t
ORIGIN      
        1 ctaaaattaa cataatacac gttatacgaa ttcccaaggc atggctttta gtcatgcttt
       61 ttttatcaaa agactatacc tgaatgatag cgagccaaat cgcatacaat atctatcatt
      121 agacttaatg caataattaa atgatagtat tatcattaat ctaatagaat taagattaaa
      181 tgatagaaat gaatacacgt atttatctac gagcttcaac caaagatcaa gatgcagaaa
      241 gggctttgca gattcttcag gatctaaacc aaaacttgaa tttgggtgaa accattgtgt
      301 acgtggaaaa ctatagtggt acgaagttag accgacctga attgaataag ctactgtcag
      361 aagcaaatca aggtgacaca ttgttagttg aaagtattga ccgcttatca cgcctaaccc
      421 aacgagattt tcaagagctg aagcgcagga tccaggagaa gggacttcgt ttagtcgtag
      481 cagatcttcc tactacctac caaatgattc aaaccagtga cagcattact cattcaatct
      541 tggaactcat caataatatg ttgattgatc ttctggcaac gatggctcgt ctggacaatg
      601 agaaacgtat agaacgtatt aggcagggcc tgacacgttc gggttacaaa ccaacaggca
      661 agaaggcaaa tgaggctaaa cataaacgaa taaaagaatt gctagtagtt ggcaatatga
      721 ctaaggaaga aattgccaaa gcagtgaatt gtggagttgc aactgtctat cgagttgctc
      781 aagttatcta aaagaggctt atctcaacgc gcccccaatg gggcgccgct gcattcaggt
      841 tacttcacat aagaaatttt atgttaaatg attgttagaa atgccccttg atcgaggcat
      901 tttctataaa gaatactaaa aactaattaa tggttttggt attaaaagaa aagtaagtat
      961 gtgnnnnnnn nnnccaagtt tcaaaacaag gcagttctca gcaatcatat ttttaaatta
     1021 tataactccc aactgagctt ttgctccaac gataaggctt tcattttgag tttctaaggc
     1081 aaataaacca tacatcaaag gagaggccgc tgctctttct aaagtctgtt catatagttt
     1141 attccaaact ttacccccta gtttttcata ctcgatgatt agagttttga ggctttcttc
     1201 tccaaacaaa gttacatgcc cagcaaaatc aatcgctggg tcatctatat gggctgttga
     1261 ccaatcaata acgcctgaaa cagctccatc ctttgaagct agtacatgcc cagcatataa
     1321 atcgccatgt ataaattggg tgaaatctgc ccatagaaca tcattatcca accattttct
     1381 gtagcgggtt tccaattgct cacttatacc aatttcagat tttactaact gcaaattgtt
     1441 tgctatttca ggtcttaaat ctgaaggttt cataattttc aaatcatttt cccgaacttc
     1501 tttttcagga atactatgga tttcaaataa ggtttttgcc aaagatgtta tgtatttcgg
     1561 gctatctttg tccatattcc aaattatttc ataggtttca gcatccaaat ttaaaacagg
     1621 attatcttta agtatgggat aagccactaa ttctgtagat gaaattctcc aatcaggaac
     1681 ctctacagaa agatgttttt ttaccaattc taaaatgcgt ttttctttct tgatttgttc
     1741 cctcatgcca tcacgacgag gaatacgcag caaccattgt tgcccctttg tatcaagagc
     1801 aaaaacgacc ttaaaatcaa tgcccatttc attgaaattc attttgtccg taagcaacaa
     1861 gccgtgtgct tcagcaagtg attgaatatc ttgaattgtc atttttaatt tcctttaaag
     1921 agttcaataa ttaatgttcg gattagattg gctatcatta acaatctctc tcaaaagtct
     1981 tgatgatttt gtggtctttg atctcgtaga taatgtcagc aatattatcg accaattgct
     2041 tgtcatgaga tacgaagata atagttcctg cataggactt catcattgtt tctaatgcgg
     2101 caatactttt taggtcaaga tagtttcctg gttcatccat aagcaaaata ttatattttc
     2161 ctaaaagcat tttggataaa agcagtttga tgatttcacc tcccgataag tcggataagt
     2221 ttttttgaat atcattcgct ccgatcccca ttgaagccaa tactgcacga atttccgcaa
     2281 ctgtgtactc gcactcttcc tgcataaagg agagcacaga tttatgcgtg ttaaatttat
     2341 atcctgtttg tgtaaagtag ccaatttcag cttttggaga tatggttaat ccatcagcac
     2401 gttctgatat catttttaac aaggacgttt tccctgttcc attcgatcca gttatagcga
     2461 ctttagcgcc aagcggtatt ataaagttag cgtcatcaaa gatagtacgg ctaccaaatt
     2521 ttaagctcag accatctgcc gtaatcggga acttattgtg cagttctagg gctgaacttt
     2581 gacgaaaacg aatagaacgc aaatgctctg gtgcttgaat atcttctaat gcagccaaac
     2641 gcttttccat actcttagct gcctgataca gttttctttg cttggtgcca gtcatttttg
     2701 catgcccaag tcgtccagca ctttcggtag agtttttgga tttttctcct tttttcttat
     2761 tgtctaatcg attagcttgc tggcgttttt cttgcacagc agattctaat cgctcccgtt
     2821 ccttcatcat cagctcatat tctacggctt ggtgttgtcg ctcttcttct ttttgacgca
     2881 agtaatccga gtaaccaccc caatattccg taattttacc gtcttttaac tcccatatct
     2941 tgtctacaac catatcaaga aaatatcggt catgactgat aacaagtaat gctccatcaa
     3001 atgctttaag ttgaccaata agtagatcta ttccattgag atcaaggtgg ctggttggtt
     3061 catccgctag aatgccatgt acttgttggg aaaatgcggc agcaattttt gcacgagttt
     3121 cctctccgcc actcattgtg tcgttttgta cattggaaac accaaggcga gataacattg
     3181 cccggtcttc gaccgtttct atttcgattc cgcccagttg gctgatatgt gcaaaatcac
     3241 caaaacgctg taatgtcgct tcggctaaaa caatttcgcc attaagtact ttgagtaaac
     3301 tactctttcc tgctccgtta tcacccacaa gaccaatacg gtcataagag tgaatttcca
     3361 attcatcaat atccaaaaca tcacgcccag cataatccaa gcgtatgttt ctcgctttaa
     3421 taattaaact catttttatt tactcctgtt tagctcttga aattttttat gcagcaaaca
     3481 ggatttaggt gaaaacaaaa gctagcatca cagtgtcctc ccaaaaaaaa agctattcat
     3541 ccacagggtg gacaaatagc tagtcaatta agttataact ggaaactatg cactaaaagc
     3601 atacttataa attaagcata cttttacttt atatatccgc atatattctt aaagacacaa
     3661 caaaagccca ccattataaa atagtgtcac tatgcaaata gttgtgtctt aacgaatgcg
     3721 gatagaatgc ataaacttac ctaaaaaata aaattcagtt ttatgataac tttactgtta
     3781 aaagaagtct agacaacctt gttttatgct tggatataag gcttatttta aaataatagt
     3841 agtgaagatt ttctaaaatt aacataatac accttatacg aaataaaaat gggagcctta
     3901 agctcccatt ttttaagtaa ttttttcaaa taaggcttgg gtgagcatct aaaattcgaa
     3961 tcaatgtagc tgctggtcca gttggatagc gtcgtccctg ttcccaattt tgcagggtac
     4021 gggggctaat atgtagacgt gcagcaaatt cattttggct caagccagtt ttttcacgta
     4081 cttctttaat atcaggcaat tcgagttctg taactcggct tgctttaact tctttacgtt
     4141 tgatagctcc agcttctttg attgaagcaa ccaagtcatc aaataagttg ttatccatga
     4201 aattgctcct tcacgagttg gtgcagaatg gcggtttcct tatctgttaa attatctttt
     4261 actgattttg gataagccac taagaaaaag atctgatcat cctcggtgac ccaataataa
     4321 atcacccgta tcccaccact tttccctttg ttaccttgag cacaacgtac tttgcgaata
     4381 ccaccgccat tcttgattag gtcacctcta tcaggctgta ccaaaagatc ttgctgaagt
     4441 tgacgatatt cctcatcaga tacaaggtct ttaatttgct tggtaaagat gctggtttca
     4501 ataaataaca tggactcttt atacgtcaat ggcgtatttg aattttagca gtttccataa
     4561 caaaaaacga gagctggtag atctcgtttc gcataacagc cattatgtta aattgtagta
     4621 agaactttaa tgcgatagag cagaatgtat atgcaaaata agcttgataa cattattcaa
     4681 gaattaaaag aactatctgg gaatagtcgt ctaaacatag aactacctga tgatattttc
     4741 atatctgctt atgaacgaaa aataggtttt atttttccaa aagattataa aaaagttttg
     4801 aaagagatta gcaatatctt ttatggaaca attgaattag cgtctcttac aga
//
LOCUS       DAOMTA010000124         4853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_427_length_4853_cov_2.206336, whole genome shotgun sequence.
ACCESSION   DAOMTA010000124 DAOMTA010000000
VERSION     DAOMTA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_427_length_4853_cov_2.206336"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..374
                     /locus_tag="PLK68_04705"
     CDS             174..374
                     /locus_tag="PLK68_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89745.1"
                     /translation="MVWNLVTDAITVILAMKVDKKAAQVVDEVLMTDNKIVFCSCHKK
                     NIKYFYLIILNKVSDYKEIEKK"
     gene            420..1265
                     /locus_tag="PLK68_04710"
     CDS             420..1265
                     /locus_tag="PLK68_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89746.1"
                     /translation="MEYKHELVIPNRSIPVKIFSFSAHNDSRIIPAHWHRSAEILYVK
                     RGKLNVWMNEQKYVLKEADFIYINSKEVHSTQSPENNEVIVLQIPGDFLETFSNRESL
                     MIHCNTIDIKQCQEFDEIRKLLCSIYSYSLGKNEVYYLKVYSLVFELGYILIKKFRVK
                     NETLDIQSKKYLDLLSEICTYIKINYQDNLTLNNVSSQFGYSPQYLARIFKKYTGQTF
                     LIYLNSVRLNYAYKEMMNSDLSLLTIAENNGFSSVKSFSKIFKNIYGEVPSVYRKKIN
                     NIGQK"
     gene            1396..>2443
                     /locus_tag="PLK68_04715"
     CDS             1396..>2443
                     /locus_tag="PLK68_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89747.1"
                     /translation="MKKIELLFEKVSPFMNKLANNPTLKGISNGMMGSIIITIVGSLC
                     LLITVFPVDAVKNFVESIGIKSVLVGVNSVTIKCLALYITVMVTNSLVKSYDPNEDGL
                     IAGMISLICFFIVTPLGQTTNEVIALPITWLGAAGVFSGLIISIITAKMFLLIKHKGW
                     TIKMPSSVPPMVSKTLEGLIPGVFISIIFIVVSCIFSLTSYGSMHQFVYSFIQTPLQG
                     LGGSLGAMCIFTIVAQLLWFFGIHGTNVISPIYTPIWLSMDLANQAAVAQNGLGSGDN
                     IIGLAFFNTFTWGGCVIGFVFLMAFCAKSKQIKSIGRLSLIPAIFGVTEPVVFGAPLV
                     LNFTLFIPFVFGNVI"
     assembly_gap    2444..2453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2454..2682
                     /locus_tag="PLK68_04720"
     CDS             <2454..2682
                     /locus_tag="PLK68_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM89748.1"
                     /translation="ISYAAIAIGLVPTLMGTTTVFGLPLGFFAAIQGSWEIIVLQLVV
                     MIIVGVFWYPFFKKYDKDMCRQEQENLAIND"
     gene            2698..>4853
                     /gene="bglX"
                     /locus_tag="PLK68_04725"
     CDS             2698..>4853
                     /gene="bglX"
                     /locus_tag="PLK68_04725"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HRM89749.1"
                     /translation="MKQQELKKLLNSLTLDEKIGQLVQLNGNFFDSNEKVATGPITKI
                     GINKEKIQLTGSILNTMGANELKRLQDKYLAKSRTKIPMLFMADILNGFKTIFPIPLG
                     LGCSFEPELVKKTAQIAAKEAAVSGIHVTFSPMVDMARDARWGRVMETYSEDNLLNCL
                     YSKAMVEGYQGDHNPYSTISACVKHFAGYGAPIAGRDYNTVELSERTLREFYLPSYKA
                     AVDAGCDLVMASFNTIDGIPVTANSRLLRNVLRDEWGFEGVVISDHSGVKELICHGIA
                     EDEKEAAKLALEAGCDIDMMTATYSNYLKELVENHQLDERYIDEAVMRVLELKNKLGL
                     FENKYRGANEELEKKYIMCEEFRQFAKEVVTKTCVLLKNDSVLPLNRDNKIALVGPYG
                     MSKDVCGMWSINVDSKQAVTVFDGLKKKYSQVSCAKGAGVIDDDSLFESFNKYSSTEN
                     IIVEDQQGLIHEAIEIAKDSDAIIVAIGEHPQQSGEGGSRGELSIAPSQIRLLKEMHK
                     LNKPIVTLIFSGRPLALKDVSDNSDAILQCWFPGTEAGDGIAEIICGEVNPSARLTMT
                     FPYSVGQCPIYYNEFSTGRPAKTSKHSRRFTSRYIDIPNDPYYCFGYGLSYSCFCYGE
                     IQLDQSIMTKTTKIKACIEVTNTSSVEGIETVQLYIRDLVGSVVRPVKELKDFKKITL
                     QAGETKKVEFTIDDNMLKFVRADYSFDSEPGKFELY"
BASE COUNT         1700 a          566 c          921 g         1656 t
ORIGIN      
        1 caataacctt tctgcggtag tcaaagctat gggaagaaag caaaagtaca cattaacata
       61 ttttaatttt acatattaaa tagaccgtaa ttaaccacac gtatatagaa ttgatagaaa
      121 agaataagtt aattacaata tattagtaaa gagtatgata taggatgatc tttttggtgt
      181 ggaatcttgt gactgatgca ataactgtta ttttagctat gaaagtagat aaaaaagcag
      241 ctcaagttgt tgatgaagtt ttaatgacag acaataagat tgttttttgt tcgtgtcata
      301 aaaaaaatat aaaatatttt tatctcatta ttttaaacaa agtaagtgat tataaagaga
      361 tagaaaaaaa ataaaaaaaa gtgtatactt ttaaatatac aatataaggg atggatatta
      421 tggaatataa acacgagtta gttataccaa atagaagtat accagttaaa atatttagtt
      481 tttcagcaca taatgattca agaatcatac cagcacattg gcatcgttct gctgaaattt
      541 tatatgtaaa aagaggcaaa ttaaatgtct ggatgaatga acaaaaatat gttcttaaag
      601 aagctgactt tatttatatt aactctaaag aagttcattc aactcaaagc ccagaaaaca
      661 acgaggttat tgtgttacag atacctggag attttttgga gacattttca aatagagaat
      721 cattaatgat tcattgtaat acgattgata ttaaacaatg tcaagaattt gatgaaatca
      781 gaaaacttct ttgtagcatt tattcttata gtttaggaaa aaacgaagtt tattatttaa
      841 aggtttattc tttggtgttt gaacttgggt atattctaat aaaaaaattt cgtgttaaaa
      901 atgaaacatt ggatattcaa agtaaaaaat atttagattt attatcagag atatgcactt
      961 atataaagat aaattatcaa gataatttga ctttaaataa tgtttctagt caatttggat
     1021 attcaccaca atatctagca agaatattta aaaaatatac gggtcaaaca tttttgatat
     1081 atttaaacag cgttagatta aattatgcat ataaagaaat gatgaattca gatttgtcac
     1141 ttttaacaat tgctgaaaat aatggatttt caagtgttaa atcttttagt aaaatattta
     1201 agaatatata tggggaagta ccgagtgtat atcgtaaaaa aataaataat ataggtcaaa
     1261 aataattgat ttaatatatc aggttaaatt ttgacctttt tttagttaat aatgagaatg
     1321 tatattctta aatttactat tataataatt ttgtaatgtt aagcgctaac atatatatat
     1381 aaggaggaga atataatgaa aaaaatagaa ttactatttg aaaaggtaag tccatttatg
     1441 aataaacttg caaacaatcc aacattaaaa ggtatttcga atggaatgat gggttcgatt
     1501 attattacga tcgttggttc tttgtgtttg ttaataacgg tatttcctgt tgatgcggtt
     1561 aaaaattttg ttgaaagtat tggaataaaa tctgtattgg taggagttaa ttcagttacg
     1621 attaaatgtt tggcattgta tattacagtt atggttacaa attctttggt gaaatcgtat
     1681 gatcctaatg aagacggttt gattgcgggt atgatttctt tgatctgctt ctttattgtg
     1741 acacccttag gtcaaacaac aaatgaggtt atagccctac caattacttg gttaggggca
     1801 gcaggagtat tttccggttt aattatttct ataataacag caaaaatgtt cttattaatt
     1861 aaacataaag ggtggacaat taaaatgcct tcatcagtac caccaatggt atctaaaact
     1921 ttagagggct taattcctgg tgtatttatt tctattattt ttattgttgt tagttgtatt
     1981 tttagtttga cttcttatgg aagtatgcat caatttgttt attcatttat tcagactcca
     2041 ttgcaaggat taggtggaag tcttggtgca atgtgtattt ttacaattgt tgcacaatta
     2101 ttatggttct tcggaattca tggaacaaat gttatttctc caatctatac acctatctgg
     2161 ttatctatgg atttagctaa tcaagcagca gtggcgcaaa atggcttagg atcaggtgat
     2221 aatattattg gattggcttt ctttaatacg tttacttggg gaggatgtgt tattggattt
     2281 gtttttctca tggctttttg tgcaaaatca aaacaaatta aaagtattgg acgtttgtca
     2341 ctgataccag ctatatttgg tgtgacagaa cccgtcgtat ttggagctcc attggtatta
     2401 aattttacat tatttatacc ttttgtattt gggaatgtta ttgnnnnnnn nnnaatttca
     2461 tatgctgcta ttgcaattgg acttgtacca acattaatgg gtacaacaac agtatttggg
     2521 ttacccctag gtttttttgc agccattcaa ggtagttggg aaattattgt attacaatta
     2581 gtagttatga taattgttgg ggtgttttgg tatccatttt ttaagaaata tgataaagat
     2641 atgtgtcgtc aagaacaaga gaatttggca attaatgatt aaatcgggag gtttataatg
     2701 aaacaacaag aattaaagaa acttttaaat agcttaacat tagatgaaaa aattggacaa
     2761 cttgtacagt taaatggtaa tttctttgat agtaatgaaa aagtagcaac tggtcccatc
     2821 actaagattg gaatcaataa agaaaagatt caattaactg gatctattct aaatactatg
     2881 ggcgctaatg aacttaaaag gttgcaagat aaatatttgg caaaaagtcg taccaagatt
     2941 cctatgttat ttatggctga catacttaat ggttttaaaa ctatttttcc tattccgtta
     3001 gggttaggct gttcttttga accggaactt gtcaaaaaaa cggcacaaat cgcagcaaaa
     3061 gaggcagcag tttctggtat acatgttacg ttttctccaa tggttgatat ggcaagagat
     3121 gcacgttggg gtagagttat ggagacatat agtgaagaca atcttcttaa ttgtttgtat
     3181 tcaaaagcta tggtagaagg atatcaagga gatcataacc catattctac aatttctgcg
     3241 tgtgtaaaac attttgcagg ttatggagcc cctattgcgg gacgcgatta taatacagta
     3301 gaattgagtg aaaggacgtt aagagaattt tatctaccct cttacaaagc tgctgtagat
     3361 gcaggatgtg accttgttat ggcatcattt aatacaattg atggtattcc agtgacagca
     3421 aactcaagat tattaagaaa tgttttaaga gatgaatggg gatttgaagg cgttgttatt
     3481 tctgatcatt ctggggtgaa agaattaata tgtcatggga tagcggaaga tgaaaaagaa
     3541 gctgctaaac tagcattgga ggctgggtgt gatatagata tgatgactgc tacttattca
     3601 aattacttaa aagagttagt tgaaaatcat caattagatg aaagatatat tgatgaagcg
     3661 gttatgaggg tactagaatt aaaaaataaa ttaggtcttt ttgaaaataa atatcgaggg
     3721 gcaaatgagg aattggaaaa gaaatatatt atgtgtgaag agtttagaca gtttgcgaaa
     3781 gaagttgtta caaaaacatg cgtcttatta aaaaatgatt ccgttttgcc tttgaataga
     3841 gataataaaa tagcacttgt tggaccatat ggtatgtcta aagatgtttg tggaatgtgg
     3901 tccattaatg ttgactcaaa acaagcagtt acagtatttg atgggttgaa aaagaaatat
     3961 agccaggtga gttgtgcaaa aggtgcaggt gttatagatg atgatagttt gtttgaaagt
     4021 tttaataaat actcatctac tgaaaatatt atagttgagg accagcaagg acttatacat
     4081 gaagctattg agatagctaa ggattctgac gctattattg ttgcaattgg tgagcatcct
     4141 caacaaagtg gtgaaggagg atcaagaggt gagctttcaa ttgctccatc acaaatacgt
     4201 ttacttaaag aaatgcataa attaaataaa ccaatcgtta cactgatttt tagtggtaga
     4261 cctttagcat taaaagatgt ttctgataat agtgatgcaa tattgcaatg ttggttccca
     4321 ggtactgaag ctggtgatgg gattgctgaa attatttgtg gtgaagttaa tccgagtgca
     4381 aggttgacaa tgacttttcc atattcagtt gggcagtgtc caatttatta taatgaattt
     4441 agtacaggaa gacccgcaaa aacaagtaag catagccgta gatttacttc acgatatatt
     4501 gatattccta atgacccata ttattgtttt gggtatggat tatcgtatag ttgcttttgc
     4561 tatggagaaa ttcaattaga tcaatctata atgactaaaa ctacaaagat aaaagcatgc
     4621 atagaagtta caaatacttc cagtgttgaa ggtattgaaa ctgttcaact ctatataaga
     4681 gatttagtgg ggtctgttgt tcgccctgtt aaagaattga aagactttaa aaaaatcact
     4741 ttacaagcag gagaaacaaa aaaggttgag tttactattg atgataatat gctgaaattt
     4801 gttagagctg attattcctt tgatagtgag ccaggaaaat ttgaacttta tat
//
LOCUS       DAOMTA010000125         4852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_429_length_4852_cov_2.191995, whole genome shotgun sequence.
ACCESSION   DAOMTA010000125 DAOMTA010000000
VERSION     DAOMTA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_429_length_4852_cov_2.191995"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLK68_04730"
     CDS             complement(<1..776)
                     /locus_tag="PLK68_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM89750.1"
                     /translation="MNIFSRLDNLTDLTTNEQTLVDYMKNNPERFINMSADEISAACF
                     ISIPTIYRLCKKLELNGLAQLKVMVSTSIRDYLKEKKTIDYNYPFSQNETQYQITMKM
                     KELYEQTLIASNNLIDLDQLRLIASALKNAQFIDMYTSAGNLYFVENFKFQMSEIGRF
                     VNVPVEEYQQLLAAASSDKEHIAIVVSFEGRGMIVDKIVKLLKKNNTPIILISSTTLK
                     SLVSLCDYNLYLSPYENHYNKISSFSTRLSLLYLLDCIYT"
     gene            complement(804..1172)
                     /gene="crcB"
                     /locus_tag="PLK68_04735"
     CDS             complement(804..1172)
                     /gene="crcB"
                     /locus_tag="PLK68_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRM89751.1"
                     /translation="MLECLAVGIGGFIGAVSRYLMGLIIIKDTTFPFMTMMINIIGAF
                     VIGLVVALASKYNLESRWILFLKTGVCGGFTTFSTFSLESMNLLSDGKFLMAGGYILL
                     SVSLCLLFVYLGNLCVKVLL"
     gene            complement(1240..>2467)
                     /locus_tag="PLK68_04740"
     CDS             complement(1240..>2467)
                     /locus_tag="PLK68_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM89752.1"
                     /translation="QQSVFEGSAWQYDEDTEMYYLHLYTKEQPDLNWENEKVRNEVYK
                     MMEWWLDKGIDGFRMDVINQISKDFEKMDKAIINDPHMYEIISNGPRVHEFLQEMHDR
                     VLAKYDTMTVGETADVSVEDALLYAGFDRRELKMIFQFEHMSLDKGPNLTYQRPKLAD
                     LKVVFERWQTGLNGKAWNALYWDNHDRPRAVSKYGDDSTPFYLEKSAKMLALFMFWMQ
                     GTPYIYQGEEIGMTNAYYQSMTQYRDVDALNKYEVFKQNYDEETIIAYFGKRSRDNAR
                     MPIPWDNSRFGGFSIVQPWLAPSQKYTNITVENALKDPNSVYYFYRDLLRLRKEYEVL
                     IYGDYQQLLKEHLQVYAYRRTLNEQQVIVICNYSKDEVEIDLEFIQGTLLISNYEDDL
                     KQILRSYEAKAYLAGK"
     assembly_gap    2468..2500
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2501..2870)
                     /locus_tag="PLK68_04745"
     CDS             complement(<2501..2870)
                     /locus_tag="PLK68_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM89753.1"
                     /translation="MQKSWWQEAAVYQIYPRSFQDSNNDGIGDLQGIISRLDYIKNLG
                     VDVIWLCPVYQSPNYDNGYDISDYQDIMADFEELLKQAHQKGLKIIMDLVVNHTSFKH
                     RWFVESRKSKDNEYRDYYIWR"
     gene            complement(2948..3733)
                     /locus_tag="PLK68_04750"
     CDS             complement(2948..3733)
                     /locus_tag="PLK68_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89754.1"
                     /translation="MQHLYEVVETSFKYPIKIWNHQFNRSKVMKPHWHDEIELIYCNQ
                     GSFELSVNGEKKFVNTKQLVLINSNSIHRLRFLEDQTKIITLLLSTELFDEFKLVQCE
                     FDLSLIKDQEQLEQLIIYLGDHLDQETLVIHNRMMQIYELLLEECSVYKLAITKKKLP
                     IIKQIVTYIEQHYDDELNLTILANHFHFSEVYLSRMFKEKTGITLLSYINKIRLNHAY
                     NDLMNTSLTINEIALNHGFKNVKAFNKIFKEYYHDIPSKYRKG"
     gene            complement(3759..>4413)
                     /locus_tag="PLK68_04755"
     CDS             complement(3759..>4413)
                     /locus_tag="PLK68_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM89755.1"
                     /translation="RNRCGNLLAKRDDVQPDSVAGVPDSGIAHAVGYSNVSGIPFARP
                     FIKYTPTWPRSFMPSSQSQRNMIAKMKLIPVKELIKNKSLLLIDDSIVRGTQLGETTE
                     FLYESGAKEVHVRPACPPIMFGCKYLNFSRSSSELDLVTRRVIQKLEGDNVSDEILKE
                     YADPDSERYKNMCEEIRRRSKFTSLRYHRLDDMIESIGLDKCKLCTYCWDGKEDEDC"
     assembly_gap    4414..4423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4424..>4852)
                     /locus_tag="PLK68_04760"
     CDS             complement(<4424..>4852)
                     /locus_tag="PLK68_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM89756.1"
                     /translation="EELKDDCFAKGATHFLEMSSGEVNPTELVAALISQKDSIVEGIK
                     YVQDVVKGSMSIMILTSEGIYASRDKLGRTPVIIGEKPEGYCATFESSAFLNLGYHHY
                     RDLGPGEIVFMTPSKIEVKQGPGDKMKICSFLWVYYGYPT"
BASE COUNT         1621 a          944 c          580 g         1664 t
ORIGIN      
        1 catgtataaa tgcaatctaa caaataaagt aatgataacc ttgttgaaaa tgaagaaatc
       61 ttattataat gattttcata tggacttaag taaagattat aatcacaaag actaactaat
      121 gactttagcg tcgttgaaga aattaatatg attggagtat tgttcttttt caacaattta
      181 acaattttgt caacaatcat tccccgtcct tcaaagctaa caacaattgc aatatgttcc
      241 ttgtcacttg aggcagctgc taaaagctgt tgatattctt caaccggaac attaacaaaa
      301 cgaccgattt ctgacatttg aaacttaaag ttttcaacaa agtaaagatt accagctgaa
      361 gtgtacatat cgatgaactg agcatttttt agagctgaag caatcagtcg taattgatcc
      421 aagtcaatta gattattcga ggcaatcaaa gtctgctcgt ataattcttt cattttcatt
      481 gtaatctgat actgcgtctc attttgtgaa aatggatagt tataatcaat tgttttcttt
      541 tctttcaaat aatctcttat tgatgttgat accattactt taagctgtgc taaaccatta
      601 agctctagtt ttttacacag acgataaatc gttggtattg aaataaagca agcagcacta
      661 atttcatcag cactcatatt aataaagcgt tctggattat ttttcatata atcaactaaa
      721 gtctgttcat tggtagttaa atctgttaaa ttatccaatc gactaaatat attcattttc
      781 tcagccccct tatcctcgta tcattataat aacactttga cacataaatt tcctaaataa
      841 acaaataata aacataagct aacactcagt aaaatatatc ctcccgccat caaaaactta
      901 ccatcactaa gcagattcat actttctagt gaaaaagttg aaaatgttgt aaagccgcca
      961 cataccccag ttttcaaaaa taagatccag cgtgattcaa gattatactt acttgctaac
     1021 gctacaacta atccaattac aaatgcccca atgatattga tcatcatagt cataaaagga
     1081 aaagtagtat ctttaattat aattaatccc attaaatatc ttgatacagc accaataaag
     1141 ccaccaattc caactgctaa acattccaac ataattatct cctctatata atcttatttt
     1201 atgattattc aaaaatttaa gcaagtaaaa aagggaagtt tacttccctg ccaaataagc
     1261 ttttgcttca tagcttctta atatttgttt taaatcatct tcataattac taatcagcaa
     1321 agttccttga ataaattcta aatctatttc cacttcatct ttactataat tacaaataac
     1381 tataacctgt tgttcgttta atgttcttcg ataagcataa acttggagat gttcttttaa
     1441 aagctgctga tagtcaccat agatcaatac ctcatattct tttcttaatc gcagtagatc
     1501 acgataaaaa taatataccg agttagggtc ttttaaagcg ttttcaactg taatatttgt
     1561 atatttttga cttggagcga gccacggctg aacaatacta aaacctccaa acctactgtt
     1621 atcccacggt attggcatcc gagcattatc acgggaacgt ttaccaaaat aagcaatgat
     1681 cgtttcttca tcataattct gtttaaaaac ttcatactta tttaatgcat caacatcgcg
     1741 atactgtgtc attgattgat aatatgcatt tgtcattcca atttcttcac cttgataaat
     1801 atatggtgtt ccttgcatcc aaaacatgaa taatgctagc attttcgcag atttttcaag
     1861 ataaaaaggg gttgaatcat caccatattt agaaactgca cgtgggcggt catgattatc
     1921 ccaatataaa gcattccatg ctttaccatt caaacctgtt tgccaccgtt caaaaactac
     1981 ttttaaatct gctaatttag gacgctgata agttaaattt ggtcctttat ctaacgacat
     2041 atgttcaaac tgaaaaatca tcttcaattc acgacgatca aaccctgcat ataatagagc
     2101 atcttctaca ctgacatcag ctgtttctcc cactgtcatc gtatcatatt tagctaaaac
     2161 acgatcatgc atctcttgaa gaaattcatg tactcgtggt ccattagaaa taatttcata
     2221 catatgcgga tcattaataa tggctttatc cattttttca aaatctttag atatttggtt
     2281 aataacatcc atcctaaaac catcgatacc tttgtctagc caccactcca tcattttata
     2341 tacttcattt ctaacttttt cattttccca attcaaatca ggttgttcct ttgtatataa
     2401 atgtaaataa tacatttctg tatcttcatc atattgccag gccgatccct caaaaaccga
     2461 ttgctgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cacgccaaat ataataatca
     2521 cgatactcat tatctttaga tttacggctt tcaacaaacc agcgatgctt aaatgaagta
     2581 tgatttacaa ctagatccat aataattttt aaaccttttt gatgtgcttg tttcaataat
     2641 tcttcaaaat cagccataat atcctgataa tcactaatat cataaccatt atcataattt
     2701 ggtgattgat atactggaca taaccaaata acatcaaccc ctaaattttt aatataatct
     2761 aaacgactga taattccctg caagtctcca atcccatcgt tattactatc ttgaaaactc
     2821 cgcggataaa tctgataaac agcagcctcc tgccaccaac ttttttgcat tatccttcac
     2881 ctcttttata tttaaacata acaaaaggtc aaaatcttgg cccttttata ctataatagg
     2941 caaaatttta accttttcga tattttgaag gaatatcgtg ataatattct ttaaatattt
     3001 tattaaatgc tttcacattt ttaaacccat gatttaatgc aatttcatta attgtgagac
     3061 tagtattcat caaatcatta taagcatgat ttaaacgaat tttattaata taagataata
     3121 atgtaatccc cgtcttttcc ttaaacattc ttgataaata aacctcacta aaatgaaaat
     3181 gattagctaa aatcgtcaaa ttcaattcat catcataatg ctgttcaata taagtgacga
     3241 tttgtttaat tatcggtagt ttcttttttg tgattgccaa cttataaaca ctacattctt
     3301 ccagcagcag ctcatatatt tgcatcattc gattatgtat aactaaagtt tcttgatcaa
     3361 gatgatcacc taaataaata attagctgtt ctaattgctc ttgatccttt attaaagata
     3421 aatcaaattc acattgtacc agtttaaatt catcaaataa ctcagttgat aaaagtaatg
     3481 ttattatttt cgtttgatcc tctaaaaatc ttaatctatg aatactatta ctattaatca
     3541 atactaactg cttagtatta acaaacttct tttccccatt cactgatagt tcaaaagaac
     3601 cttgattaca ataaattaac tctatttcgt catgccaatg cggcttcatt actttactgc
     3661 gattaaactg atgattccaa atcttaatag gatatttaaa gctagtttca acaacttcat
     3721 ataaatgctg cattatcatg cctcacaaaa aggattgctt agcaatcctc atcttctttc
     3781 ccatcccaac aatatgtaca taatttacat ttatctaaac cgattgattc gatcatgtca
     3841 tctaaacgat gataacgcaa tgatgtaaat ttagagcggc gacgaatttc ctcacacata
     3901 tttttatatc tttcactatc tgggtcagcg tattctttta aaatctcatc actaacatta
     3961 tcaccttcta gtttttggat aacacgacga gtcactaaat ctaattctga actagagcgt
     4021 gaaaaattta aatatttaca cccgaacatg attgggggac aggcaggacg aacatgaact
     4081 tctttagccc cactttcata taaaaattct gttgtttcac ctaattgtgt tccccgaaca
     4141 attgaatcat caatcaataa taaactttta tttttaatta attctttaac cggaattaat
     4201 ttcatcttag caatcatatt tctttgactt tgactagatg gcataaatga ccgtggccaa
     4261 gtcggtgtat atttgataaa tggacgagca aagggaatac ctgaaacatt tgaataacct
     4321 actgcatggg caattcctga atccggtacc ccagcaacgc tatctggctg tacatcatca
     4381 cgttttgcta ataaattacc acaacgatta cgcnnnnnnn nnnatgttgg atatccataa
     4441 taaacccata agaatgaaca aattttcatt ttatctccag gaccttgttt aacttcaatt
     4501 ttacttggtg tcatgaaaac aatttcccct ggtccaagat cacgataatg atgatatcct
     4561 aaatttaaaa atgcactact ttcaaatgtc gcacaatatc cttctggttt ttccccaata
     4621 atcactggtg ttcttcctaa tttatcacgt gaagcataaa ttccttcaga tgttaatatc
     4681 ataatactca ttgagccttt aacaacgtct tgaacatatt taattccttc tacgatacta
     4741 tccttttgac taatcaatgc cgctactaat tctgtaggat tgacttcacc cgaactcatt
     4801 tccaagaaat gagttgcccc tttagcaaaa caatcatctt ttaactcttc ca
//
LOCUS       DAOMTA010000126         4851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_430_length_4851_cov_2.970392, whole genome shotgun sequence.
ACCESSION   DAOMTA010000126 DAOMTA010000000
VERSION     DAOMTA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_4851_cov_2.970392"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1245
                     /locus_tag="PLK68_04765"
     CDS             55..1245
                     /locus_tag="PLK68_04765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRM89757.1"
                     /translation="MKKINKKRVAFASLVIICLIIIIALIINLTILPLFNNSNGVTKN
                     KQAKAKKESYDTVSLVAVGDNIIHERVFQYASTNGTYDFTPCYKHIKKYIQDADLAFI
                     NQETILGGDTLKITGYPAFNSPSELAKNLIDTGFNMINGATNHSFDRDFEGVKAASQT
                     WRQYQDIIYTGTYDSQSDRDTIRIIEKNGIKFALLSYTQSLNEYNTNPYKLLKQTAYA
                     VPLLEDTAAIKADVQKAKEQADVIIVSAHWGDENEAEVTAKQQEYAQLFADLGVDLVI
                     GTHPHIIQPVTWVNGQSGNKTLIAYSLGNFLSTMETQDTQLEGMLSLNFIKKDAKIFI
                     DDITWTPLINHFGDNTVEVYPLAKYPDDKLAKHFVLHDKPNIIQQYKAKTRDVIGDKI
                     TIKD"
     gene            1250..2116
                     /locus_tag="PLK68_04770"
     CDS             1250..2116
                     /locus_tag="PLK68_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM89758.1"
                     /translation="MNKKRLNKKKVLLVFIPLIIAGTIIYWFLPSNSTKIEEKQPKKT
                     VNNDPQYYQTSLKERYENYQKTNPDLSTEEIITRVNMNLDYPFYEVIIPQNKPLELNT
                     IVNKYYKLDDNFTPDDLIYINDTYANTSDPAYKYRKHQMRKVVYDDFIALKEACKTKG
                     FNLYVVSGYRSTTWQTEIYNHMVNTYSVAKADQTCSHPGHSEHTTGLACDIALDNYSF
                     EDVIKHPQYQWFLGQLANYGFIIRYPENKDTLTGYSYESWHLRYLGKDLAKKVTASNL
                     TYDEYYAQNYAK"
     gene            2248..2739
                     /locus_tag="PLK68_04775"
     CDS             2248..2739
                     /locus_tag="PLK68_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89759.1"
                     /translation="MDIITNVLPFLVFLALLWFTYTNISLAVAMYRAKKDDSTLSSCL
                     SNTGKYFYIGLTIFYIALFVGSVALMVLALINNKLDDLYLPLNVLTIGTLAIGFLLQQ
                     IILVGHRQMMIGKIKLDYRKIKRVSYPKAKKLRFVYGQRTYETPLRFIDDFKLKKALQ
                     KAR"
     gene            complement(2776..3525)
                     /locus_tag="PLK68_04780"
     CDS             complement(2776..3525)
                     /locus_tag="PLK68_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89760.1"
                     /translation="MEEIIKTANLKKVYGLDTPYPKTALNGVSISVNKGTFACIMGTS
                     GSGKTTLINILSTIDEATSGKLLIFDQNIVGLSDKEKANIRKKYMGFIFQDYNLIDSL
                     KVIDNILFSLKLNKKNIINEAEIKQIISSLGIEELLDKYPFECSGGQQQRIAIARALV
                     CKPKILFADEPTGNVDSIRAKQLMEYFTEINRKYGITIVMVTHDCLVASYASEMYYVE
                     DGKIINHIFKGNDSFEKFYNRIARISMQIKL"
     gene            complement(3513..>4851)
                     /locus_tag="PLK68_04785"
     CDS             complement(3513..>4851)
                     /locus_tag="PLK68_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM89761.1"
                     /translation="KKGGIGMLQISLQMLWKERKKALNLGITITITLCVCIIFLQFFN
                     NPIINYLVNILKDTTKFYGYEAITEYEVMRFYDGMYKGTMVIILLVIFISLIMYSCNY
                     YNKTNSRIVGLLKIMGCRDREILFFQMIQLIVITLISYLIAVGISFLLIPLCQALAYL
                     YMGVSENIFYYALETYVDSLPLVLLLLVFMTFMQISYSIRGVIPDLLKNDEIVSFKKR
                     RRITIVANVNYIVLFIIGTFTIYISDLNQGLIFPAGLYVIGAYNLGVMCFPNLLEKWI
                     KLKNIDAKESLIFNNFILNLQQMKSVILMFVLSSAILLTMMCTNLDDLQYTILFQLGY
                     VLTNIVLSFTLINRFRINQINKKQYYRNLSRLGLNYEEIRYMTKKEKTLMYEMIAVLS
                     LVYLSNLNIAFVYRDKMGVLSALFLILELIIPLFISYRVAVYQEEMRIRKWKK"
BASE COUNT         1796 a          818 c          642 g         1595 t
ORIGIN      
        1 tatataataa ctatataata aaacaaatta aaactattgt atagaggtaa tcatatgaag
       61 aagatcaata aaaagagggt agcctttgca tcactggtaa taatctgctt aatcataata
      121 atcgcactta ttatcaattt aactatctta ccattattta ataattcaaa cggtgttaca
      181 aaaaataagc aagctaaagc aaaaaaagaa agctatgata ctgtatctct ggttgctgtt
      241 ggtgataata ttattcatga gcgtgttttt caatatgcca gcactaacgg tacatatgac
      301 tttacccctt gctataaaca tataaaaaaa tatattcaag atgctgatct tgcttttatt
      361 aatcaagaaa caattttagg tggtgatacc ttaaaaatta caggatatcc ggcttttaat
      421 tcaccaagtg aattagctaa aaatttaatt gacactgggt ttaatatgat caatggtgct
      481 actaatcatt ctttcgaccg tgattttgaa ggtgttaaag cggcttctca gacttggcgc
      541 caatatcaag atattattta tactggtaca tacgatagcc aaagtgatcg tgatacaatt
      601 aggatcattg aaaaaaacgg tatcaaattt gcacttttat cttataccca aagtctcaat
      661 gaatataata ctaatcctta taagttgtta aaacaaactg cctatgctgt acccttattg
      721 gaggatacag cagcaatcaa agcagatgtt caaaaagcca aagaacaagc agacgtaata
      781 atcgtctctg cccattgggg tgatgaaaat gaggcggaag taactgctaa acaacaagag
      841 tatgctcaat tatttgctga tttaggagtt gatttagtaa tcggaactca tcctcatatc
      901 attcaacctg ttacatgggt gaatggccaa agtgggaata aaacactcat tgcttacagt
      961 ttaggaaatt ttttaagtac aatggaaact caagatacac aattagaagg aatgttatca
     1021 ttaaacttta ttaaaaaaga tgctaaaatt tttatagatg acattacctg gacaccacta
     1081 atcaatcatt tcggtgataa tactgttgaa gtttatcctc ttgcaaaata tccagatgat
     1141 aaattagcta aacattttgt tttacatgat aaacctaaca ttattcagca atacaaagcc
     1201 aaaacaagag atgttattgg tgataaaata actataaagg actaaatcca tgaataaaaa
     1261 acgcttaaat aaaaagaaag tattattagt ttttattccc cttattattg ccggtactat
     1321 aatctactgg ttcttgccta gtaattcaac caaaatagaa gaaaagcaac cgaaaaaaac
     1381 agttaataac gatccccaat attatcaaac ttcgttaaaa gaacgttatg aaaactatca
     1441 aaaaactaat cccgatcttt cgacagaaga aataattacc cgagttaaca tgaatcttga
     1501 ttatcctttc tatgaagtaa tcattcctca aaataagcca ttagaattaa atacaatcgt
     1561 aaataaatat tataaattag atgataactt cactcccgac gatcttattt acatcaatga
     1621 tacttatgcc aatacaagtg atcctgccta taaatatcga aaacatcaga tgcgaaaagt
     1681 tgtttatgat gattttattg cattaaaaga agcatgtaaa actaaaggct ttaatctata
     1741 cgtcgttagt ggctatcgtt cgacaacctg gcaaaccgaa atctataatc atatggtgaa
     1801 tacatacagc gttgctaaag ctgaccaaac ttgtagccac cccggccata gcgaacatac
     1861 gaccggtcta gcttgtgata ttgcacttga taattacagt tttgaagacg ttataaagca
     1921 tccccaatat cagtggtttc ttggacaact agctaactac ggctttatta ttcgctatcc
     1981 agaaaacaaa gatacactta ccggatatag ctacgaatct tggcatcttc gctatttagg
     2041 aaaagattta gctaaaaaag tgactgctag taatttaaca tatgatgaat attacgcaca
     2101 aaattatgca aaatagcctg tttcagtact caggctatta ttttttcact aaaatttatg
     2161 atgattatta aacttttatc aatttttata taggctaatt catgattttc tagtataata
     2221 gagatattga aagaaggagt atgactaatg gatattatta caaatgtatt accttttttg
     2281 gtatttttag ccttattatg gtttacatac acaaatattt ctttagcagt agcaatgtat
     2341 cgcgcaaaaa aagatgatag taccctatca tcatgtctaa gcaatactgg taaatatttt
     2401 tacattggcc taacaatttt ctatattgcc ttatttgtgg ggagcgttgc tttaatggta
     2461 ttagcgctaa tcaacaataa attagacgac ctatatttac cactaaatgt cttaacgatc
     2521 ggaacactag cgattggttt tcttttacag caaatcatct tagttgggca ccgtcaaatg
     2581 atgatcggta agatcaaact ggattatcgt aaaattaaac gtgtttcata tccaaaagca
     2641 aaaaaattaa gatttgttta tggacaaaga acttatgaaa ctccacttcg ttttattgat
     2701 gatttcaaac taaaaaaagc tttacaaaaa gctcgttaaa ctcttatggg tagcaaaaaa
     2761 tgctaccctt ttagtttaca atttaatttg cattgatatc cttgcaattc gattataaaa
     2821 tttctcaaaa gaatcgttcc ctttaaaaat atgattaatt atcttcccat cctcaacata
     2881 atacatttct gatgcatatg atgcaaccaa gcaatcatga gtaaccataa caatcgtaat
     2941 accatattta cgattaatct ctgtaaaata ctccattaat tgcttagccc taatactgtc
     3001 tacgttccct gtcggctcat cagcaaaaag aatttttggc ttacatacaa gtgcacgagc
     3061 aattgcaatt cgctgctgct gaccccctga acattcaaaa gggtatttat ctaaaagttc
     3121 ttcaatacct aaactgctaa ttatttgctt gatttctgct tcattgataa tattcttttt
     3181 attaagtttt aaagaaaata aaatattgtc aattactttt aaagaatcta tcaaattata
     3241 atcttgaaaa ataaatccca tatatttctt tctaatattt gctttttctt tatccgacaa
     3301 cccaactata ttctgatcaa agattaataa ttttcctgaa gtagcctcat caattgttga
     3361 tagaatattt atcaatgttg ttttaccaga acctgaagtc cccataatac atgcaaaagt
     3421 ccccttattt actgaaattg aaactccatt taatgccgtt ttaggatatg gtgtatctaa
     3481 gccatacact ttttttaaat tagccgtttt aattatttct tccattttct tatcctcatt
     3541 tcctcttgat atacagcaac tcgatatgaa ataaacaatg gaataataag ctctaatata
     3601 agaaataatg cagacaaaac tcccatttta tcacgataga caaaagcaat atttaaattt
     3661 gataaatata ccaatgataa gaccgcaatc atttcataca tcaatgtttt ttcttttttt
     3721 gtcatatatc tgatttcttc ataattaaga cctaatcgac ttaaattacg ataatattgc
     3781 tttttattaa tttgattaat tctaaaacga ttaatcaatg taaagcttaa aacaatattt
     3841 gttagcacat accccaattg aaataagatc gtatactgta aatcatcaag attagtacac
     3901 atcattgtta ataaaattgc agaagataat acaaacatta aaataactga tttcatctgc
     3961 tgcagattca aaataaagtt attaaaaatt agactttctt tagcatcaat attctttaat
     4021 ttaatccact tttctaataa gttaggaaaa cacataacac ctaaattata tgctcctatt
     4081 acatataaac cggctggaaa aatcaatccc tgatttaaat cactgatgta gatagtaaaa
     4141 gtacctataa taaataatac aatataatta acgttagcta ctatcgttat tcgccttctt
     4201 ttcttaaacg agacgatttc atcattttta agtaaatcag gaataactcc gcggatagaa
     4261 tacgatattt gcataaaagt cataaaaact aatagaagca gtaccaaggg caaagaatcg
     4321 acatatgttt ctaaagcata ataaaagata ttttcactaa cccccatata aagataagca
     4381 agtgcctgac ataatggaat cagcaaaaaa ctaattccca cagcaattaa ataactaatt
     4441 aaagtgatta caatcaactg aatcatctgg aaaaataaaa tttctcgatc acgacacccc
     4501 attattttca atagaccaac aatccgtgaa tttgttttat tatagtaatt acaagaatac
     4561 attattaatg aaataaaaat cactaataaa ataatgacca ttgtcccttt atacattcca
     4621 tcataaaatc gcatcacttc gtattcagta attgcttcat aaccataaaa ctttgttgta
     4681 tcttttaaaa tatttactaa atagttaatt atgggattat taaagaattg gagaaaaata
     4741 atacatacac ataaagttat tgtaattgta attccaagat ttaaagcttt ttttcgctct
     4801 ttccatagca tctgtaaaga aatctgcagc atcccaatcc ctcctttttt a
//
LOCUS       DAOMTA010000127         4850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_432_length_4850_cov_1.903233, whole genome shotgun sequence.
ACCESSION   DAOMTA010000127 DAOMTA010000000
VERSION     DAOMTA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_432_length_4850_cov_1.903233"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="PLK68_04790"
     CDS             complement(<1..2351)
                     /locus_tag="PLK68_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM89762.1"
                     /translation="MKRKYSVKGMTCSACENHVHNSVCKLPGVEHVEVNLLTNSMNVE
                     FDETKVDDSMIIKAVKDGGYQASSYDDALVDNDDLSNLKRKLIYCFIALGLLMYVSMS
                     SMLSYPIPNIIRDNVYFNIILQIIFLIPILILKKDYFVNGFKNLIHFDPTMDSLIALG
                     AGAAIVYSIYSVVLALSGSLMEMHLVHNVYFESAGMIVTLISFGKYLEAKSKKKTTDA
                     IGKLLELAPDVACRFNDGKEEIVKISELKIDDLVLVRANETVPVDGKIVQGFSSIDES
                     MITGESLPIEKEVGSLVIGGTNNLQGTFIYTVTRTVEDSTLAKIIELVEEASSSKAPM
                     TRTIDKVVKYFVPTVIVIALITFVGWLIMGEDLNFAITSAIAVLVISCPCALGLATPV
                     AIMVSTGVGATNGILIKSAEVLENENNIDVVVFDKTGTLTKGMAQVSDIYSHKLELAQ
                     VIAIIASLEKGSSHVLANAFIQKAAEMNLELKEITDFESLSGLGIRGTIDNVEYAVGN
                     LRMMKESGIDLSRYQEKIDLYLIQGKTLVFLAHDQELIGLVSIFDDIKDTSRQAIQRL
                     KEMKIKTVMLTGDLKGTANAINKQLGLDEVIAEVLPQDKENVIQQLQAQGNSVLMVGD
                     GINDAPALVRSDVGVAIGKGNDIAIDAADVILMKDDIRDIVASIELSKRTIINIKENL
                     FWAFIYNVIGIPIAAGIFYYSFGLRLDAMVGSLCMSLSSVCVVTNALRLKRFKPYYQK
                     ENKKMKKEIVIEGMMCQHCKKHVEEALNGLADTTAAVDLENNLA"
     gene            complement(2452..2988)
                     /locus_tag="PLK68_04795"
     CDS             complement(2452..2988)
                     /locus_tag="PLK68_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM89763.1"
                     /translation="MSLDQIIKQRRSIRKYKKKPVSNEQIQLLIQAAVEAPSWKNSQT
                     ARYHVITSQPLLNEFKERCLPEFNQENCKDAPVLIVTSFIPNRSGFERDGTPSNELAN
                     GWGCYDLGLHNQNILLKATELGLGSLVMGIRDIDKIKGLLDISKEEIIVSVIAIGISD
                     IEPERPKRKTVEDITRFY"
     gene            complement(3001..4218)
                     /locus_tag="PLK68_04800"
     CDS             complement(3001..4218)
                     /locus_tag="PLK68_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM89764.1"
                     /translation="MSSVASISAIALINSKMYETVYNEARSDELTGLLNRKYFYEALN
                     NEYEKLGERQLSLILVSVDDVRLYNQLYGNKEGDIVLINVARIMDDYVNTQGYTARLG
                     GKEFGIILPNYGPLEAKDLAENIREQIMNMNKRDSDYTLKVVTASFGISSIPVSANTI
                     KQLLDYADQALYQSKRHGKNRVSVYNAGVVESTSGVTIVDAHREKQNIYLEYAPTIYA
                     LTAAIDAKDHYTFTHSENVAYYATKLAIGCGYDSELVEIINEAALLHDIGKIGISENI
                     LKKTSKLSDEEYQIMQSHVEASVNIIRHLPSLDYVIPIVLTHHERYDGRGYPRRISGE
                     DIPAGGRIMAIADSFDVMTSRRSYKEPYSLEYALNELKRNRGLQFDPDLVDKFIELIN
                     SGIIEVRGNQNNE"
     gene            complement(4182..>4850)
                     /locus_tag="PLK68_04805"
     CDS             complement(4182..>4850)
                     /locus_tag="PLK68_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM89765.1"
                     /translation="SYVIAGMLSIILFANLVGTFQNILFEHFGLITKNYYVLVIAVAF
                     TIVTAIIYTMLKKFIDAIFIKEEIQQSDSLKEFSNYVSKTLQVNEIVGAMGEIILKCV
                     ETKQVYICLLDDNGDYSIEYSSSPLRKNNYIINHENPVVIKLMNNDDMIFYSEFRHSI
                     NYRSMWDSEKQLFENLNIEGIIPVKQEQLIGMILLAPKPRRSGYSIEKLVSCLQLLQL
                     VPLL"
BASE COUNT         1607 a          989 c          578 g         1676 t
ORIGIN      
        1 gcaagattat tttctaaatc aactgcagca gttgtgtctg ctaagccatt taaagcttct
       61 tcaacatgtt ttttacaatg ttgacacatc attccttcaa ttacaatttc ctttttcatc
      121 ttcttattct ccttttgata gtatggttta aagcgtttta atcgtaacgc attagtaaca
      181 acacaaactg atgataatga catgcataaa gaaccaacca tggcatctaa tcttaatcca
      241 aaactataat agaaaatacc ggctgcaatt ggaatcccta tcacattata aataaatgcc
      301 caaaataaat tttctttaat attaataatt gttctttttg ataattcaat cgatgccact
      361 atatcacgaa tatcatcttt catcaagata acatcggctg catctatcgc aatatcattt
      421 cctttaccga tagcaacacc cacatcacta cgaactagcg caggagcatc attgattcca
      481 tcaccgacca ttaaaacact attgccctgt gcttgcaatt gttggattac attttcttta
      541 tcttgtggca agacttcagc aattacctca tctaaaccta attgtttatt aatagcattt
      601 gcagtacctt ttagatcacc agttagcata actgttttaa ttttcatttc ttttaaacgc
      661 tgaattgctt gacgacttgt atctttgata tcatcaaaaa tactcaccaa ccctatcagc
      721 tcttgatcat gagctaaaaa tactaatgtt ttaccttgaa ttaaatataa atcaattttt
      781 tcttgatatc gacttaaatc aattccactc tctttcatca tccttaaatt accaactgca
      841 tattcaacat tatcaattgt tcctctaatt cccaaaccac ttaatgattc aaaatcagta
      901 atctctttta attctaaatt catctcagca gctttttgaa taaaagcatt cgctaaaaca
      961 tgactagaac ccttttctag cgatgcaatg atcgcaatta cttgtgctaa ttctaatttg
     1021 tgactataaa tatcactaac ttgagccatt cctttagtta gagtccctgt tttatcaaaa
     1081 acaactacgt caatattatt ttcattttct aatacttctg cacttttgat taggatccca
     1141 ttagttgctc caactcctgt tgacaccatg attgctactg gagttgccaa accaagagca
     1201 catggacaag aaataactaa taccgcaatt gcgctagtaa tagcaaaatt caaatcttct
     1261 cccattatca accatcctac aaaagtgatt agggctatta cgattactgt aggaacaaaa
     1321 tatttaacaa ccttatcaat agttctagtc attggtgctt tggaactaga tgcttcttca
     1381 accaattcaa taattttggc taatgtacta tcctcaaccg ttcttgttac agtatatata
     1441 aaagtacctt ggagattatt tgttccccca atcactaaac tacctacttc tttttcaatc
     1501 ggaagacttt caccagtaat cattgattca tcaatagaac taaatccttg tactatctta
     1561 ccatcgactg gcactgtttc atttgccctt actagaacca gatcatcaat ttttaattcg
     1621 gaaattttaa caatttcttc tttgccatca ttaaaacgac aagcgacatc aggagccaat
     1681 tctaataact tcccaatcgc atcagttgtt ttcttctttg atttagcctc aagatatttc
     1741 ccaaaactaa tcaatgtaac aatcatacca gcagattcaa aataaacatt atggactaaa
     1801 tgcatctcca ttaaagatcc gcttaaagct aaaacaactg aataaatact atagacaata
     1861 gcagctcccg caccaagtgc aatcaacgaa tccatagttg gatcaaaatg gatcaaattc
     1921 ttaaaaccat taacaaagta atcttttttc aaaatcaaaa ttggaatcaa aaagattatt
     1981 tgtaatatga tattaaaata aacattatcc ctaataatat tagggattgg ataactgagc
     2041 attgatgaca ttgatacata catcagtaat ccaagtgcga tgaaacaata aattaatttc
     2101 cttttcaaat tacttaagtc gtcattatca actaatgcat catcataact actcgcctga
     2161 tatccaccat cttttacggc tttaataatc atgctatcat caacttttgt ttcatcaaat
     2221 tcaacattca ttgaatttgt taataaattc acctcaacat gttcaactcc cggtaattta
     2281 caaacactat tatgaacatg attttcacaa gctgaacaag tcatcccttt tacactatat
     2341 ttacgtttca taagacctcc tcccccctcc tagggggtac ggatatagta tagcccaaaa
     2401 taaaatttta tgcaaaagaa atgcacaaaa aaactattac aaaagtaata gttaataaaa
     2461 tcttgtaata tcctcaaccg ttttacgttt aggccgttct ggctcaatat cactaatacc
     2521 tatagcaata actgaaacaa ttatttcttc tttagaaata tctaacaatc ccttgatttt
     2581 atcaatatcg cgaatcccca tgaccaatga tcctaatcct aattctgtcg cttttaaaag
     2641 aatattttga ttgtgaagtc caagatcata acagccccag ccatttgcca gttcgttact
     2701 aggcgttcca tcacgttcaa atcctgagcg atttggaatg aaagatgtta ctattaaaac
     2761 cggtgcatct ttacaatttt cttgattaaa ctccggtaaa catctctctt taaactcatt
     2821 caataaaggt tgtgatgtaa taacatgata tcgagctgtt tgactatttt tccaagatgg
     2881 tgcttcgact gctgcttgaa taagcaattg tatctgctcg ttagaaacag gcttcttttt
     2941 atattttcta atacttcggc gctgcttaat aatttggtct aatgacatta tttttcctcc
     3001 ttactcatta ttttgattac ctcttacttc aattatacca gaatttatca gttcaataaa
     3061 cttatcaact aaatcgggat caaattgcaa tccccgatta cgctttaatt cattaagagc
     3121 atactctaat gaatatggtt ctttataact ccttcttgaa gtcattacat caaaagaatc
     3181 cgctattgcc ataattcttc ctccggccgg gatatcttca ccagaaattc ttcttggata
     3241 tccacgtcca tcataacgtt cgtgatgtgt taagacaatt ggaataacgt agtccaatga
     3301 aggtaaatgt cgaataatat taactgaagc ttccacatgc gattgcataa tttggtattc
     3361 ttcatcactt aatttagaag ttttctttaa aatattttct gaaatcccaa ttttaccaat
     3421 atcatgaaga agtgcagctt cattaataat ctcgactaat tcactatcat atccacatcc
     3481 gattgctaat tttgtagcat aataagcaac attttcagaa tgggtaaaag tataatggtc
     3541 ttttgcatca attgcagctg tcaatgcata aattgtcggc gcatactcta agtaaatatt
     3601 ctgtttttct cgatgtgcat caacaatcgt cacaccactt gtactttcaa ccactcctgc
     3661 attataaact gatacacgat ttttaccatg tcgctttgac tgatatagcg cttgatctgc
     3721 ataatctaat aattgcttaa ttgtattggc agataccgga atagaactaa taccaaagct
     3781 tgcagtaact acttttaaag tatagtcact atctcgttta ttcatattca ttatttgctc
     3841 gcgaatgttt tcggccagat cttttgcttc taaaggtcca taatttggta aaataatccc
     3901 aaattccttt ccccctaaac gtgctgtata accttgtgta tttacataat catccataat
     3961 tcgtgctaca ttaattaaga caatatctcc ttctttatta ccatacaatt gattataaag
     4021 ccgaacatca tctacactta ctaaaatcaa tgataattgg cgttctccca atttttcata
     4081 ttcattattc aaagcctcat aaaaatattt acgattcaac agtccagtta attcatcact
     4141 acgtgcttca ttataaactg tctcatacat ttttgaatta attaaagcaa tggcactaat
     4201 tgaagcaact gaagacaaga aactaatttc tctatactat accccgagcg ccgtggtttg
     4261 ggagccagca ggatcatccc aattaattgc tcttgtttaa ctgggataat tccttcaata
     4321 tttagatttt caaataattg tttctcactg tcccacattg aacgataatt tattgaatgt
     4381 ctaaattcac tataaaatat catatcatcg ttattcatta atttaatcac cacaggattt
     4441 tcatgattaa taatatagtt attttttctt aacggtgaac tgctgtactc aattgaataa
     4501 tcaccattgt catctaaaag acaaatatac acttgtttgg tttcgacaca tttcaaaatt
     4561 atttcaccca ttgccccgac aatttcattt acttgcaaag tttttgaaac atagttagaa
     4621 aactctttta aactatctga ttgctgaatt tcttctttta taaaaattgc atcaataaat
     4681 ttttttaaca ttgtataaat tatcgcagtt acaatcgtaa aagctactgc aattactaat
     4741 acataataat ttttagtaat caaaccaaaa tgttcaaata aaatattttg aaatgtacca
     4801 actaaattag caaataaaat aattgacaac atcccagcta ttacataact
//
LOCUS       DAOMTA010000128         4838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_434_length_4838_cov_2.181267, whole genome shotgun sequence.
ACCESSION   DAOMTA010000128 DAOMTA010000000
VERSION     DAOMTA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_434_length_4838_cov_2.181267"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..991)
                     /gene="pfkA"
                     /locus_tag="PLK68_04810"
     CDS             complement(32..991)
                     /gene="pfkA"
                     /locus_tag="PLK68_04810"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM89766.1"
                     /translation="MVKCIGVLTSGGDAPGMNAAVRAVARTCLNKGIEVYGVRLGYKG
                     LYEGDFIKFDRHSTRNIINLGGTILKSARFPEFKDPEIRKVAIEQMKKVGMEALVVIG
                     GDGSYNGALKLTEMGVNCIGIPGTIDNDIPDTDFTVGFDTALNTIVDALDRLRDTSSS
                     HQRCTILEVMGRRCGDLAINAGIADGAEMIITSEIGFDESKVIERLKASKESDKKHAI
                     VVITEHITDVHELAKKVEAATGFETRANVLGHMQRGGRPSARDRVLASRMGVYAVELL
                     EAGKGGLCVSQVNGEIKGLDIEETLSHKRKTDFGIYEDAMKLR"
     gene            complement(1099..2007)
                     /locus_tag="PLK68_04815"
     CDS             complement(1099..2007)
                     /locus_tag="PLK68_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM89767.1"
                     /translation="MKSIQDINLFNNDDSFNKNKENSINTLMNDHNIIKVLQEFNLSR
                     RDIEENWIEFLDYKEDLDVCIGCKSLKSCPKISKGMVRLFNYQDGEVKLSLQPCRYGQ
                     AHFDDQKILNDILLKNVNNKILLTKPSDLTIIDDPNGNGRVVIKMMMDYIKNPTNKGF
                     YLYGEGGNGKSTVMGFLIRCLVTKGYKCGYIHFPTFLMDLKSSFGNEGVNSSIELMKN
                     LDYLVIDDVGGESVTSWSRDEILSAIIAYRLQNQRATFFTSEYSLEKLKKLYTLKAGD
                     KERVERLISRMKAVSIPVELKGKDLR"
     gene            complement(2017..3261)
                     /locus_tag="PLK68_04820"
     CDS             complement(2017..3261)
                     /locus_tag="PLK68_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRM89768.1"
                     /translation="MSELMMSDLYTVNASYPLDSIHMRSIQLLYQPLIGNNACGLYLS
                     LYAELDQLSLTKALSLHSRLTKITGLSLTELNEASLKLEAIGLVSKYIKDSDNKRVYL
                     YALKTPLTPTKFFNHAILGPLLLQRLGQEELYRTKLCFKSSAFSIEGFNNVTAKFTDV
                     FDINLLNGCDDVLVEKDYFNTNYGSIESDYDLTLFYQGLADYQIKRSCITPDDEAVIK
                     QLGTLYRINAIDMQGLVKDCLQNDKLIHSALISKCRDYYDLNMPETFKEIYHKQAIVH
                     KSVTGDDALSKHISYLESINPYQLLKDKQGGREPLKHDLQIVESIMTSLYLEPGVMNV
                     LIELTLSQCDNALSRAFMQARASQWKRKKIKTVKDAMDEANVYLKYRRNNNDDNEETE
                     INMVDNDNVDMDELDEFLSQFE"
     gene            complement(3263..3832)
                     /gene="coaE"
                     /locus_tag="PLK68_04825"
     CDS             complement(3263..3832)
                     /gene="coaE"
                     /locus_tag="PLK68_04825"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792773.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRM89769.1"
                     /translation="MKVLGITGSIATGKSTVTNYLKQRGYLVVDSDKLAYDALTIDEV
                     CIKQTKNRFDLPAGPIDRKALGRIIFNDKQAKKDLEAIIHPYVIKKMQEIIVLNQHLD
                     LIFLDIPLLFESNLEYLCDAVIVVYLKEDEEIKRLMKRDNIDEDYARLIIGNQMSIEE
                     KKMRADIVLDNNQGLDELYQQIETLLKGR"
     gene            complement(3833..4780)
                     /locus_tag="PLK68_04830"
     CDS             complement(3833..4780)
                     /locus_tag="PLK68_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM89770.1"
                     /translation="MINWGIVGAGRIAHRFCEALAQDSRANLEAVSCRTLEKAKAFQE
                     KHPCNKAYDSFQAVLDDPEIEAVYIALPHLYHFEWVKKAILANKKVLVEKPATMNTAE
                     MEEIKDLVKKHHILFMEAMKTRFVPGYQEAKSLVSEGIIGELECLETSFCGKVDYNQN
                     SYLFDIKQGGCLLDLGIYNIAYLDDYFKVPLDTVDVTCKYHDCGVDTYVKAILNFGKQ
                     TGIVECAMDRNKENQAVLTGTLGKMIIRPIHRPLDLEVILNSGEIFKYHIEYDHDDFY
                     SEIVHFNDLIENGQVESTIMSLDHSINCAKILEAVREMM"
BASE COUNT         1574 a         1004 c          550 g         1710 t
ORIGIN      
        1 taattttcct caccttacat ttttatttat attaacgaag cttcattgca tcttcgtaaa
       61 taccaaaatc agttttacgt ttatgtgata atgtttcttc aatatctaat cctttgattt
      121 ctccattgac ttggcttaca cataaaccac ctttaccagc ttctaataat tccactgcat
      181 atacacccat gcgagaagct aaaacacgat cgcgagctga aggacgtccc cctctttgca
      241 tatgccctaa aacgttcgca cgagtttcaa aacctgttgc agcttctact tttttagcta
      301 attcgtgaac atcagtaata tgttcagtaa taactacgat tgcatgtttt ttatcagatt
      361 cttttgatgc ctttaatctt tcaattactt ttgattcatc aaaaccaatc tcactagtaa
      421 taatcatttc agccccatca gcaattccag cattaatagc taaatcacca caacgacgtc
      481 ccattacttc taagatagta caacgttgat gagaacttga agtatctctt aaacgatcta
      541 atgcgtcaac aattgtattt aaagctgtat caaaaccaac agtaaaatca gtatctggaa
      601 tatcattatc aattgttcct gggataccaa tacagttaac acccatttca gtaagtttta
      661 aagctccatt ataacttccg tctccaccaa taacgaccaa agcttccatc cctacttttt
      721 tcatttgttc aattgcaact tttctaattt caggatcttt aaattccggg aaacgtgctg
      781 atttcaaaat cgttccccct aagttaataa tattacgagt actatgacga tcaaatttaa
      841 tgaaatctcc ttcatataac cctttgtatc ctaggcgtac tccgtatact tcaatacctt
      901 tatttaaaca tgttcttgcc actgcacgaa cagctgcatt cattccagga gcatctccac
      961 cagatgttaa tacaccaata catttaacca ttgtttttcc tcctaataac cacattttta
     1021 tttccaagac attttaacac aaaaagattg atgtttccat caaatcttta atattttaca
     1081 ctaaaactca aacttttttt agcgtaaatc cttacctttt aattctactg gaatactaac
     1141 cgctttcatt cttgaaatta gtcgttcgac tctttctttg tctccagctt ttaaagtata
     1201 aagctttttt agtttttcca aagagtattc actagtaaaa aaagtagctc tttgattttg
     1261 tagccgataa gcaattattg ctgataatat ctcatcacgt gaccatgacg taactgactc
     1321 accaccaaca tcatcaataa ccaaataatc taaatttttc atcagctcaa ttgaactatt
     1381 gactccctca ttaccaaaag aactttttaa atccattaga aaagttggaa aatgtatata
     1441 accacattta tatcccttgg ttacaagaca ccgaatcaag aagcccatta ctgtactttt
     1501 accatttccg ccttcaccat ataaataaaa gcctttatta gtaggatttt taatataatc
     1561 catcatcatt ttaataacta ctcgtccatt gccattagga tcatcaatga ttgtcaagtc
     1621 actcggcttc gttaataata ttttattgtt aacatttttt aataaaatat cattcaaaat
     1681 cttttgatca tcaaaatgtg cctgaccata acggcaaggc tgtaatgaaa gctttacttc
     1741 gccatcctga taattaaata gacgaaccat tcctttagaa atcttagggc aagattttaa
     1801 tgatttacag ccaatacaaa catccaaatc ttctttataa tcaagaaatt caatccaatt
     1861 ttcttcaata tcacgacgac ttaaattaaa ctcctgcaat accttaataa tattatggtc
     1921 attcattaaa gtattaatac tattttcttt atttttatta aaactatcat cattattaaa
     1981 caaattaata tcttgaatac tcttcatctt gccctcctat tcgaactgac ttaaaaattc
     2041 atctaactca tccatgtcta cattatcatt atcaaccata ttgatttcag tttcttcatt
     2101 atcatcgtta ttattacgac gatattttaa gtatacatta gcttcatcca tcgcatcttt
     2161 aactgtcttg attttttttc gtttccactg tgaagctcgt gcttgcataa acgctcttga
     2221 taaagcatta tcacattggg ataatgttaa ctcaattaac acattcatca caccaggttc
     2281 tagatacaat gaagtcatta tcgattcaac gatttgtaaa tcgtgcttta aaggctctcg
     2341 ccccccctgt ttatccttta acagttgata agggttaata ctttctaaat aactaatatg
     2401 cttacttaac gcatcatcac ctgtaacact tttatgaaca attgcctgct tatgataaat
     2461 ttctttaaat gtttctggca tattcaaatc ataataatca cggcatttgc taatcaaagc
     2521 actgtgaatt aatttatcat tttgaagaca atctttaact aatccttgca tatcaattgc
     2581 attaatacga tacaaagttc ctaactgttt gattacggct tcatcatcgg gagtaataca
     2641 tgatcttttt atttgataat cagcaagccc ttgatagaac aatgttaaat cataatcact
     2701 ttcaatactc ccataattag tattaaagta atctttttca accaggacat catcacaccc
     2761 atttagtaaa ttaatatcga atacatcagt gaacttagca gttacattat taaatccctc
     2821 aatactaaac gctgatgatt taaaacataa ttttgttcga tatagctcct cttgccctag
     2881 acgttgtaat aggagtggtc ctagaatagc atgattaaaa aatttagttg gtgttagggg
     2941 tgtttttaaa gcatataagt atactctttt attatcacta tctttaatat atttagaaac
     3001 taatcctatc gcttcaagtt ttaaactcgc ttcattaagc tcggttaaac ttaaaccggt
     3061 tattttagtt aaacggctat gaaggctaag ggctttagtc aatgataact gatctaattc
     3121 agcataaaga cttaaatata atccacaagc attattacca atcaaaggtt gatatagtag
     3181 ttgaatacta cgcatatgaa tactatcaag aggataactt gcattaaccg tatataaatc
     3241 agacatcatt aactcgctca tcttatcttc ctttcaataa tgtttcaatt tgttgatata
     3301 attcatcaag accctgatta ttatctaaaa caatatccgc tctcattttc ttttcttcaa
     3361 tactcatttg gttaccaatg atcaaccttg cataatcttc atcaatatta tcacgcttca
     3421 tcaatctttt tatttcttcg tcctctttta aatatacaac aattactgca tcacataaat
     3481 attccaaatt actttcaaat aatagcggta tatctaaaaa aattaaatca agatgctgat
     3541 taagcacaat tatttcttgc attttcttga ttacataagg atgaattatt gcttccaaat
     3601 ccttttttgc ttgtttatca ttaaaaataa tccgacctaa agctttacga tcaattggtc
     3661 cagctggtaa atcaaagcga ttttttgttt gtttaataca cacctcatca atagttagtg
     3721 catcgtaagc taatttatca ctatcaacca ctagatagcc tcgctgtttt agataattcg
     3781 tcaccgtact tttaccagtt gcaatactgc ctgttattcc taaaaccttc acttacatca
     3841 tctcccgtac tgcctctagt atcttagcac aattgatgct atggtcaaga ctcattattg
     3901 tactttcaac ctgaccattt tctattagat cattaaaatg tacgatttca ctataaaaat
     3961 catcatgatc atattcaatg tgatatttga atatttcacc actatttaaa ataacttcta
     4021 aatcaagcgg ccgatgaatt ggtcgaatta tcattttacc aagcgttcct gtcaatactg
     4081 cctggttttc tttatttcga tccatcgcac attcaacaat accagtttgt ttcccaaaat
     4141 ttaaaatagc ttttacatat gtatcaacac cacaatcatg atatttacaa gtaacatcga
     4201 cagtatccaa aggtacttta aaataatcat ctagataagc aatattataa atacccaaat
     4261 ctaataaaca accaccctgc tttatatcaa acagataaga attctgatta taatcaactt
     4321 taccgcaaaa actagtttca agacattcaa gttcaccaat aattccttca ctaaccaaac
     4381 tttttgcttc ttgataacca ggtacaaaac gagttttcat cgcttccata aataaaatat
     4441 gatgcttttt aactaaatct tttatttctt ccatttctgc agtattcatt gtagctggct
     4501 tctcaactaa aactttctta ttagcaagaa tagctttttt gacccattca aaatgatata
     4561 aatgcggtaa ggcaatataa accgcttcaa tttcaggatc atccaaaaca gcttgaaaac
     4621 tatcataagc tttattacat ggatgttttt cttgaaaagc ttttgccttc tctagcgttc
     4681 gacaactaac ggcttctaaa ttagcacgtg aatcttgagc cagagcttca cagaaacgat
     4741 gagcgatccg accagctcca actattcccc aattaatcat tttaccttct ccttttttga
     4801 caatgcttac agtaataagt cccacgacct tttattgc
//
LOCUS       DAOMTA010000129         4827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_438_length_4827_cov_2.300503, whole genome shotgun sequence.
ACCESSION   DAOMTA010000129 DAOMTA010000000
VERSION     DAOMTA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_4827_cov_2.300503"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLK68_04835"
     CDS             <1..342
                     /locus_tag="PLK68_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HRM89771.1"
                     /translation="QEQRIVAISDIVSALCGKRSYKTSFSKEKTCEILNQMAQAGEID
                     SMIVAQVIDNYDLIEGKIWKEAAAVLEKYNTIIVTYSDLLNRKQELIEAKWLYLVFRY
                     SKTGIVNLKID"
     gene            443..1078
                     /locus_tag="PLK68_04840"
     CDS             443..1078
                     /locus_tag="PLK68_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM89772.1"
                     /translation="MAKRLGLYFLGILILGFGIVLNTKTGLGVAAINSVPFGIREMTN
                     LSLGMATTILYIIFVGVQLLIYQKLDFKVLLQIPFSYFMGYVLDFYNNLLNFTVTSLP
                     VALVLLAIAILATALGAYVVVTLDLIPNPADGMVKALSQVIDKEFGKTKLLFDCGMML
                     VTIITTYCLAGKIIGIGIGTILSAGFIGQIIVIYNRYFTKHLTLIVENSRA"
     gene            1177..2718
                     /locus_tag="PLK68_04845"
     CDS             1177..2718
                     /locus_tag="PLK68_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HRM89773.1"
                     /translation="MNGYQLRIDVEGCADPIWRRIKIPAIISFEDLHQLIQTLFGFED
                     YHLYEFRIKELGIWIPGGETDIENQINDDVEIVDNLERIADYLKKEMVIYYHYDFGDD
                     WQLLITVEQELEGVEHYPVVVEFCGNNLIEDCGGIERYQEIILSRDDLDINFDLEDIN
                     SCLSLLGVDEILNNSPLKNEFIEILEQLKELVKKREFTDNQVIKLVSNQTTYWVILKT
                     LEGYVIELFETYNDLLEGFYNLANEGINNAFCNCWTILLSEQELDFDVALDEDNYLAA
                     FRNEAGYIPCLLEVEEARVILELLKEFANGIKEDQNRSESDEIIEIYVEDGKFKESAI
                     FVHEPQIDLNRYDFGYLGEHELETKRENTERLCVDVVCLPMTNTDKTNELNVYAVIAG
                     ESDYLIKEVDFPSLQIIGEALIDGLLEYANRYGKPQRIVVNNLNVLFLVLNFISQYDI
                     DYFEDEITLEIEGAIMDAFGLETADEAFNDPFIQELLEQLDGKSEEEIEEKINEILAN
                     MELLN"
     gene            2819..>3637
                     /locus_tag="PLK68_04850"
     CDS             2819..>3637
                     /locus_tag="PLK68_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRM89774.1"
                     /translation="MMNCIREVNENVYWIGGNDRRLSLFENIFPIPRGISYNAYVVLD
                     EKTILLDTVDWSIGHLFFDNLETALQGRSLDYIVINHMEPDHCACLKEVINRYPEVVI
                     VGNAKTFTMIDQFFGIEINKLVVKENDTLTTGKHTFTFVMAPLVHWPEVMVTYDSYDK
                     TLYSADAFGTFGALDGALFNDEVDFEHEWLDDARRYYANIVGKYGPQVQMLLKKASSL
                     EIATICPLHGPVWRNNIDYIVSKYDCWSKYEPEEKGVVLAYGSIYGNTENVMEII"
     assembly_gap    3638..3647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3858..4199
                     /locus_tag="PLK68_04855"
     CDS             3858..4199
                     /locus_tag="PLK68_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89775.1"
                     /translation="MYDVSKVHVSNLISEVFHYSHLILAAPTYNSGSFPPMENFLSDM
                     IALSLKKRSVALLENGTWGALCAKHMRTKLETMKDMEIINEPITIKSTLKTEQVDDID
                     NLVTTIVASIG"
     gene            4288..4746
                     /locus_tag="PLK68_04860"
     CDS             4288..4746
                     /locus_tag="PLK68_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89776.1"
                     /translation="MDIDGLRNMMFVFKTKSKRNIIQLFNFRSSKANNIDETQIKEVN
                     DYLYIPIDLKNWLDIDTNKCLERVLTTLLHLTDPKSGRPGASIATIVAGYDENHQSNF
                     IFTTDYIDGKHTVIGYYENGDEVIYRDSIVLRGKNCLDKYNDLSSKWQIK"
BASE COUNT         1681 a          483 c          952 g         1701 t
ORIGIN      
        1 caagagcagc ggattgtagc aataagtgat attgtaagtg cactatgtgg aaagcgcagt
       61 tataaaactt cttttagtaa agaaaagact tgtgaaattt taaatcaaat ggcacaagca
      121 ggagaaatag actcaatgat cgttgctcaa gtaattgata actacgattt aatcgaggga
      181 aaaatctgga aagaggctgc ggcagtttta gaaaagtata atactattat tgtgacatat
      241 agtgatctat tgaacagaaa acaagagttg attgaagcaa aatggctata tttagttttt
      301 cgatattcaa aaactggaat tgtcaattta aaaattgatt gatgaagtat tcttcttttg
      361 gaaggatatt ttttcttttg ttgaaaaaga actgataata tcaagtaata ttgtaaaata
      421 aagaaaaata aggagggtgt ttatggcaaa aaggttagga ttatattttt tagggatatt
      481 gattttagga tttggaattg ttttaaatac aaaaactggt ttaggggttg cagcaattaa
      541 ttcagttcca tttggaatta gagaaatgac taatttatca ttagggatgg caacaactat
      601 tttatatatt atttttgttg gagtgcaatt attgatttat cagaaattag attttaaagt
      661 tttattacaa attccatttt cttattttat gggatatgtt ttagattttt ataataactt
      721 attaaatttt acagttactt cattacctgt tgcattagtt ctattagcga ttgctatttt
      781 agctactgct ttaggagcat atgttgttgt aacgttagat ttaattccta atcctgctga
      841 tggtatggtc aaagcattaa gccaggtgat tgacaaagag tttggtaaga caaaattatt
      901 atttgattgt ggaatgatgc ttgtcacaat tattactaca tactgtttgg cggggaaaat
      961 tattggaatt gggataggaa caatattatc tgctggattt attggccaaa ttattgtgat
     1021 ttataatcgt tatttcacaa aacatttaac attgattgtt gaaaatagtc gagcgtgata
     1081 attatttata aatccctttt atcttctcaa tataaaggcg ttacttcaat aatataattt
     1141 gttataatag tttatataga actgaggaga taaaagatga atggatatca attgaggata
     1201 gatgtggagg ggtgtgcgga tcctatatgg cggcgtatta aaatacctgc aataattagt
     1261 tttgaagatc tgcatcagtt aatccaaact ttatttggtt ttgaagatta tcatctatat
     1321 gaatttagga tcaaggaatt agggatctgg attcctggtg gtgaaacaga tattgaaaat
     1381 cagataaatg atgacgttga gatagttgat aatcttgaac ggattgcgga ttatttaaaa
     1441 aaagaaatgg taatttacta ccattatgac tttggtgatg attggcaact tttaattacg
     1501 gttgaacaag agttagaggg cgttgagcac tatcctgttg tagttgaatt ttgtggtaat
     1561 aatttgatag aggattgtgg gggaattgaa cgttatcaag aaattatatt aagtcgagat
     1621 gatctggata ttaattttga tctagaagat attaatagtt gtttatcatt gttaggggtt
     1681 gatgaaatat taaataatag cccgttaaaa aatgagttta ttgaaatttt agagcagtta
     1741 aaagaattag tgaagaaacg tgagtttact gataatcaag tgattaaact agtatctaac
     1801 caaactacat attgggttat tttaaaaaca ttagaaggtt atgtgattga attatttgaa
     1861 acttataatg atcttttaga gggtttttat aatcttgcta atgaaggaat aaataatgca
     1921 ttttgcaact gctggactat tttattatct gaacaggaac ttgattttga tgtggcatta
     1981 gatgaggata actatttggc tgcttttcgt aatgaagcgg gatatattcc atgcttatta
     2041 gaagttgagg aagcaagggt aattttagaa ttattaaaag aatttgcaaa tgggattaaa
     2101 gaagatcaaa atcgtagtga gtctgatgaa attattgaaa tatatgttga agatggtaaa
     2161 tttaaggaga gtgctatttt tgttcatgaa ccacaaattg acttaaatcg ctatgatttt
     2221 ggctatttag gtgaacatga gcttgaaact aagcgtgaaa atacagaacg tttatgtgtt
     2281 gatgttgtct gtttaccaat gactaataca gataaaacca atgagttaaa tgtttatgcc
     2341 gtaattgccg gagaaagcga ttatttaatt aaagaagttg attttcctag tttacaaatt
     2401 attggtgaag ctttaattga tggattgctt gaatatgcta atcgttatgg taaaccacaa
     2461 cgaatcgtag taaataattt aaatgttctt ttcttagtct tgaattttat tagtcaatat
     2521 gatattgatt attttgaaga tgagataacg ctagaaattg aaggggcgat aatggatgct
     2581 tttggccttg aaactgctga tgaggcattt aatgatcctt ttattcaaga attgttggag
     2641 cagttagatg gtaaaagtga agaagaaatt gaagaaaaaa taaatgagat attagctaat
     2701 atggagctgc taaactaatc tttgagggat aatagtttag ggtttggaaa taatataagc
     2761 atcatattag taaaattaga atattaggtt tatactaaaa gtgatttagg aggctgtgat
     2821 gatgaattgt attagagaag taaatgagaa tgtttattgg attggcggga atgatcgtcg
     2881 cttaagctta tttgaaaata tttttccaat tccaagggga atttcatata atgcttatgt
     2941 tgtgttagat gaaaagacca ttttattaga tacggttgat tggtcgattg gacacttatt
     3001 ttttgataat cttgaaacag cattgcaagg aagatcattg gattatattg ttattaatca
     3061 tatggagcct gatcattgtg catgtttaaa agaagtgata aatcgttatc ctgaagtcgt
     3121 gattgtagga aatgctaaaa catttacaat gattgatcaa ttttttggga ttgagatcaa
     3181 taagttagta gttaaagaga atgatacttt aactacagga aaacacactt ttacttttgt
     3241 gatggcacca ctcgtacatt ggccagaagt aatggtgaca tacgatagtt atgataaaac
     3301 attatatagt gctgatgctt ttggaacatt tggagcatta gacggagctt tatttaatga
     3361 tgaagttgat tttgaacatg aatggcttga tgatgctagg agatattatg ctaatatcgt
     3421 tggaaaatat ggaccacaag ttcaaatgtt attaaaaaaa gctagtagtt tagaaattgc
     3481 cacaatttgt ccgcttcatg gacctgtatg gcgtaataat attgattaca tagttagcaa
     3541 atatgattgt tggtctaaat atgaaccaga agaaaaaggt gttgttttag cttatggttc
     3601 gatctatggt aatactgaaa atgtaatgga aataattnnn nnnnnnnctg gtttaatcca
     3661 ttttcttgtg tgataattca tctacattat tttttcaagc tacaaaatta tgctataaat
     3721 atgaactgag cgttagttta aaatactttc ttttcattga ttttattgaa ggaaactata
     3781 attgttttaa tgtgaatttt ctgtttttac gaataattgc ttcaaaattg aagcaagccg
     3841 gggtaaaaaa tgttagaatg tatgacgtat ctaaagtgca tgtttctaat ttaattagtg
     3901 aagtgttcca ttatagtcat ttgattttag cagcgccaac atataatagt ggaagttttc
     3961 caccaatgga aaacttcctt agtgatatga ttgctttatc attaaagaaa agaagtgtag
     4021 cgttattaga aaatggaaca tggggagcat tatgcgcaaa acacatgcgt actaaattag
     4081 agacgatgaa ggatatggaa atcatcaacg aaccaatcac aattaaatca actttgaaaa
     4141 ctgagcaagt ggatgatatt gataatttag ttactacaat agttgcttca attggataat
     4201 agaaagccgg tgatcattat gatcaggctt ttttattttc taataagtta tatatttatt
     4261 atataactta ttagaaggag aacaattatg gatatagatg gtctaagaaa tatgatgttt
     4321 gtttttaaaa caaaatcaaa gaggaatata attcagttat ttaattttcg aagctcaaaa
     4381 gcaaataata ttgatgaaac tcaaattaaa gaagtaaatg attatttata tattccgata
     4441 gatttaaaga attggcttga tattgataca aacaaatgtt tggagagagt tttaacaacg
     4501 ttgttgcatt taacagatcc taaaagtggt agacctggcg catcaatagc gacgattgtg
     4561 gcaggatatg atgaaaatca tcagtcaaat tttattttta caactgatta tattgatgga
     4621 aagcatactg ttataggtta ttacgaaaat ggtgatgagg ttatttatcg tgacagtatt
     4681 gttttaagag gaaagaattg tttagataag tataatgatt taagtagcaa atggcaaatt
     4741 aaataaaaag tagacgttga aataaatcta tatggtttat tttaatgtct actttatttt
     4801 attaataatt agctaatgat actcttt
//
LOCUS       DAOMTA010000130         4826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_440_length_4826_cov_1.920981, whole genome shotgun sequence.
ACCESSION   DAOMTA010000130 DAOMTA010000000
VERSION     DAOMTA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_440_length_4826_cov_1.920981"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1690)
                     /locus_tag="PLK68_04865"
     CDS             complement(109..>1690)
                     /locus_tag="PLK68_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89777.1"
                     /translation="VTQGSLKEIRDTMFEHMEKLPIRYFDTHNHGDIMSIYTNDTDTL
                     RQMISQSVPQVIVSATTIVCVLISMIVMSIPMTIISVLMVCVMLLVSKHVTNRSGRYF
                     FAQQTNLGKVNGFIEEMMEGQKVVKVFTHEEEAKFDFDKVNEELFESAYQANKYANIL
                     MPLIGNLGYVSYVLVAVVGGVLAINGYMDLTVGTLAAFLQLNRSFNQPIGQISQQINM
                     VLMALAGAERIFALMDEEVEDDLGHVSLVNAKENADGTLSPVKERTGLWAWEHKRPDG
                     SIEYVRLAGDVRFHDVTFGYNENKTILYDMNLFAKPGEKLAFVGATGAGKTTITNLIN
                     RFYDIQKGSITYDGIDIKLIKKADLRRSLGIVLQDTHLFTGTVMDNIRYGKLDATDEE
                     CIEAAKLANAHEFIMHLEHGYQTILSGDGSSLSQGQCQLLAIARAAVANPPVLILDEA
                     TSSIDTRTESIVQSGMDKLMEGRTVFVIAHRLSTIKNSDAIMVLDQGRIIERGDHDKL
                     IKEKGTYYQLYTGGLELD"
     assembly_gap    1691..1700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1701..1965)
                     /locus_tag="PLK68_04870"
     CDS             complement(<1701..1965)
                     /locus_tag="PLK68_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89778.1"
                     /translation="MSNSRGKTMKRLFKFVVKRHKKTCFAILLLIIVSSIASVMGTIF
                     IKSLIDDYITPYINMANPDFGPLTNAILKMIAIYAVGVIATFSY"
     gene            complement(1958..3691)
                     /locus_tag="PLK68_04875"
     CDS             complement(1958..3691)
                     /locus_tag="PLK68_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89779.1"
                     /translation="MLKTLLAQIKEYKKDTILTPVLVVVEVILEVVIPLLMAMIIDKG
                     IEVRDMNMVVKLGIITLLASFISLAAGGLAGKYAAKASTGFAKNLRKAMYYNIQDFSF
                     ANIDKYSTAGLVTRMMTDVTNVQNAFQMLIRACIRAPLMMVSAMIMAFTINAQIAMVF
                     LVSIIFLGVLLVFFMTRAHPYFKRVFNTYDDLNASVQENVNGIRVVKAYVREEHEDEK
                     FKKTSTLIYKLFVKAENYLVFNMPLMQLTVYGCIIGISWFGAHLIVGGNLTTGELTSL
                     FTYVMMILMSLMMFSMVFVMVVMSIASAQRISEVINEKSTLHNPAEPDYEIKDGSIDF
                     NNVNFSYFDDQEEINLRDINVHIKSGQTIGIIGGTGSAKSTFVQLIPRLYDVTKGEVL
                     VGGKDVRKYDLETLRNEVAMVLQKNVLFSGTIKENLRWGNKEASDEEIVEACKLAQAD
                     EFIQRFPDKYDTYIEQGGTNVSGGQKQRLCIARALLKKPKILILDDSTSAVDTKTDAL
                     IRSAFKKVIPGTTKLIIAQRISSVEDADLIIVLDDGQISAMGTNDELLESSAIYKEIY
                     ETQKKGGTLSE"
     gene            complement(3747..4175)
                     /locus_tag="PLK68_04880"
     CDS             complement(3747..4175)
                     /locus_tag="PLK68_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM89780.1"
                     /translation="MPNKKIGFLIKQVFYLHEQHLNKQFSKFGLTASQTFTLIYLFKS
                     HDKGISVNQKDIEKELDISNPTVTGILNRLEVKGLITRVPCRHDARAKNIEVTEKALE
                     LDKQLRIVFQQSDEKLVESLSKEEIDNLQSYLIKILRSNS"
     gene            complement(4303..>4826)
                     /locus_tag="PLK68_04885"
     CDS             complement(4303..>4826)
                     /locus_tag="PLK68_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM89781.1"
                     /translation="VLIFVADYQKWYDAFKYYHGEEQMRSPSYGDFLLAFSDTLIAAQ
                     NAVVAAESLGIGSCFIGDIIEQYEVHRELLNLPQYTAPVGMLVMGYPTEQQQHRTKPA
                     RFDSKYVVSVDRYEKLVKMFDDRAVLANRSSGGRSFVDDIYNRKWTDPFIEEMSRSSK
                     LWIEQWGKEDEES"
BASE COUNT         1645 a          963 c          604 g         1604 t
ORIGIN      
        1 actggatatt tagtaggtac tttagatcgg ttcaatgatg ctaaagctgc tgaagaaaaa
       61 gatcgggtca aacatgcata aaaaagcagc tcaaatcgag ctgctttttt aatctaattc
      121 taagccacca gtgtataact gataataagt tcctttttct ttaatcaatt tatcatggtc
      181 accacgttca atgatccttc cttgatcaag taccatgatg gcatcagaat ttttaattgt
      241 tgataaacga tgagcaataa caaaaaccgt tcgtccttcc atcagtttat ccattccact
      301 ttgaacaata ctttctgttc tcgtatcaat acttgatgtt gcttcatcta agatcaatac
      361 cggtggatta gcaaccgcag cccgagcaat agctagcagc tgacattgtc cttgtgataa
      421 acttgaacca tcgccactca aaatagtttg atacccatgt tctaaatgca taataaattc
      481 atgagcattg gctagttttg cagcttctat acactcctca tcagtagcat caagtttacc
      541 ataacgaata ttatccatta ctgtaccagt aaataaatgt gtatcttgca aaacgattcc
      601 taacgatctt ctcaaatctg cctttttaat taatttaata tcaattccat cataagttat
      661 tgaacctttt tgaatatcat aaaaacggtt gatcaaattc gtaatagttg ttttaccagc
      721 tcctgtagct ccaacaaaag caagcttttc accaggttta gcaaataaat tcatatcata
      781 caaaattgtt ttattttcat tatatccaaa tgtaacatca tgaaaacgaa catcaccagc
      841 taaacggaca tattcgattg aaccgtctgg tcgcttatgc tcccaagccc aaagtccagt
      901 acgttcttta actggagata atgtaccatc agcattttct ttagcattaa ctaaagaaac
      961 atgaccaaga tcatcttcaa cctcttcatc cattaatgca aagattcttt ctgcacctgc
     1021 caatgccatc aacaccatat ttatttgttg agaaatttgt ccaattggct gattaaaaga
     1081 gcggtttaat tgtagaaagg cagctaatgt ccctaccgtt aaatccatat agccattaat
     1141 cgcaagaaca ccacctacaa ccgcaactaa cacataactt acgtatccta agtttccaat
     1201 taacggcatt aaaatattag catatttatt tgcttgatat gcactttcaa ataattcttc
     1261 attgacttta tcaaaatcaa atttagcttc ttcttcatga gtgaagactt taacaacttt
     1321 ttgtccttcc atcatttctt caataaaacc attaacttta cctaaattag tttgttgagc
     1381 aaagaaatac cgcccactac gattagttac atgtttagaa actaataaca ttacacaaac
     1441 catcaaaaca ctaattatcg tcattggaat agacattaca atcattgaaa taagaacaca
     1501 aacgatcgtt gttgctgaaa caataacctg aggtacactt tgcgaaatca tctgtcttaa
     1561 agtatcagta tcattagtat agattgacat gatatcccca tggttatgag tatcaaaata
     1621 acgaatcggt aatttttcca tatgttcaaa catcgtatca cgaatttctt ttaacgatcc
     1681 ctgagtcact nnnnnnnnnn tataactaaa agtagcaatt actcctactg cataaattgc
     1741 aatcattttc aaaatcgcat tcgtcaacgg tccaaaatca ggatttgcca tattaatata
     1801 tggtgtaata taatcatcga ttaagctttt gataaagatt gttcccataa cactagcaat
     1861 actactaaca atgattaata acaatattgc aaagcaggtc tttttatgtc ttttaacaac
     1921 aaatttaaac aatcttttca ttgtttttcc ccgactatta ctcacttaaa gttcctccct
     1981 tcttttgtgt ttcataaatc tctttataaa ttgcactaga ctccaatagt tcatcattag
     2041 tacccattgc actgatctga ccatcatcta aaacaatgat taaatcagca tcttcaactg
     2101 aactaattct ttgagcaata attaattttg ttgttccagg gataactttt ttaaacgcac
     2161 tacgaatcaa tgcatctgtt ttagtatcaa ctgcactagt tgaatcatct aaaattaaaa
     2221 ttttgggttt ctttagcaat gcacgagcaa tacacaatct ttgcttttgc cccccagaaa
     2281 cattagttcc gccttgttct atataggtat catatttatc tgggaaacgt tgaataaact
     2341 catctgcctg agctaattta caagcctcca caatttcttc atcactagct tctttattac
     2401 cccagcgcaa gttttcttta attgttccag aaaacaaaac atttttttgt aataccattg
     2461 caacttcatt tcgcaatgtt tccaaatcgt attttctaac atctttaccg ccaactaaaa
     2521 cttctccttt agttacatca tataaccgtg gaataagctg aacaaaagtt gatttagcac
     2581 taccggttcc accaataatc ccgattgttt gtccagattt aatatgaaca ttgatatcac
     2641 gtaaattaat ttcttcttga tcatcaaaat atgagaaatt aacattatta aaatcaattg
     2701 aaccatcttt aatttcataa tcaggctcag caggattatg cagcgttgat ttttcattga
     2761 ttacttcact aatacgttgg gctgaagcaa ttgacataac taccatgaca aataccattg
     2821 aaaacatcat taaactcatt aaaatcatca ttacataggt aaataaacta gttaattctc
     2881 cagtagtaag atttcccccg acaattaagt gtgctccaaa ccaagaaatt ccaataatac
     2941 atccatatac agttaactgc attagcggca tattaaaaac taaatagttt tctgccttta
     3001 caaatagttt ataaatcaaa gttgatgtct ttttaaattt ctcgtcttca tgttcttcac
     3061 gaacataagc tttaacaacc cgaattccat taacattttc ttgaactgaa gcgtttaaat
     3121 catcatatgt gttgaatact cttttaaaat acggatgagc tctagtcata aagaaaacta
     3181 aaagaactcc taaaaagata attgacacta aaaaaaccat agcaatctga gcattgatcg
     3241 taaaagccat gatcatggca ctaaccatca tcaatggggc acggatacaa gctcttatta
     3301 acatttgaaa agcattttgg acattagtaa catccgtcat cattcttgta accaatccag
     3361 cagttgaata tttatcaata ttagcaaaag aaaaatcttg aatattgtaa tacattgcct
     3421 ttcttagatt tttagcaaaa cctgttgagg ccttagcggc atatttacca gccaaacctc
     3481 ctgccgctaa agaaataaag gaagcgagca atgtaatgat tcctaattta acaaccatat
     3541 tcatatctct aacttctatt cccttatcaa tgatcatcgc cattaaaagt ggaattacaa
     3601 cttccaaaat aacttcaacc acaacaagta caggtgttaa gatcgtatct tttttatact
     3661 cttttatttg ggctaaaagt gtttttaaca tttatatacc tccctaaaaa ttaaatagcg
     3721 tgctatgaat aacagtaaaa taaatattaa gaattacttc ttaatatctt aatcaaataa
     3781 ctttgtaaat tgtctatttc ttcttttgat aatgattcaa ctaatttttc atcagactgt
     3841 tggaaaacaa tccgtagctg tttatccagt tctaaggcct tttcagttac ttcaatattt
     3901 ttagcacgag catcatgacg acacggtacc cgggtaatta atccctttac ttctaaacga
     3961 ttcaaaattc cagtaactgt aggattagaa atatctaatt ctttttcaat atctttttga
     4021 ttcacactaa ttcctttgtc gtgagattta aacaaataaa ttaatgtaaa tgtctgtgaa
     4081 gcagttaatc caaatttact aaattgttta tttaaatgct gttcatgtaa ataaaaaact
     4141 tgtttgatta aaaatccaat ttttttattt ggcattttac cacctccaaa ataaatagca
     4201 cgctatatat tatatgcaaa tattagcaaa tgtcaactat ctttcaataa ttatttatca
     4261 tctttataag agctgataaa cgataaaaag ctaagaatca tcttagcttt cttcatcctc
     4321 ttttccccat tgttcaatcc ataatttaga tgaacgagac atttcttcaa taaatggatc
     4381 agtccatttg cgattataaa tatcatcgac aaaacttcgt cccccactgc tgcgattagc
     4441 taaaactgct cgatcatcaa acatttttac taacttttca taacgatcaa cactaacaac
     4501 atatttagaa tcaaaacgag caggcttagt tcgatgttgc tgctgttctg tcggataacc
     4561 cattacaagc atccccactg gagctgtata ttgaggtaaa tttaataatt cacgatgtac
     4621 ttcatactgc tcaatgatat caccgataaa acaacttcct attcctaaac tttcagctgc
     4681 aacaacggca ttttgagctg caatcaaagt atcactaaat gctaataaaa aatcaccata
     4741 tgatggcgaa cgcatctgtt cttcaccatg ataatattta aatgcatcat accatttttg
     4801 ataatcagca acaaagatta aaacta
//
LOCUS       DAOMTA010000131         4825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_442_length_4825_cov_2.710692, whole genome shotgun sequence.
ACCESSION   DAOMTA010000131 DAOMTA010000000
VERSION     DAOMTA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_4825_cov_2.710692"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1767
                     /locus_tag="PLK68_04890"
     CDS             256..1767
                     /locus_tag="PLK68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM89782.1"
                     /translation="MAFIKKIKKLKKSVKITGGVIIGVIVVFAIFSFYYRDKWYPNTS
                     INGIDVSSMTYEESKNVLKNQIESYALEIGAEGNQKLTIAGKDINLSADFEKNLESDY
                     KNHRSQRSIFGIFSGYNHDIDLKISYDQNKLSEIVNGSVLINGNEEYQIVQSTNAHIE
                     YDETTKSGKMVKATIGNELNLEKFSNLITTSISKLTTKIDLTDQDKYAEVYQQPVSDI
                     SDKHLEEMLNTYNNYLLNWINWDMGEGKVETMTPDDIKNWLSCNDKGEVVLDKEAMSE
                     WIEEFCLRYKTVGKKRNFTTHNGNVIQISGGDYGWRLDYEKIVKQVEAAITEKTDSKL
                     IEAYLSEQSKKNQKALTTELEPTYSNKAYQKDYENFENDWDTQNYSEIDLTEQRVYVY
                     RDGQLAYSCICVSGLPTEKNDRITRTGVWYIKEKKPEKVLVGEDYETPVKYWIRIMWT
                     GTGYHALDRSDWANWTPDLYKVKGSHGCLNLQEEDAKKLYELIRMNDPVFIHY"
     gene            1970..2362
                     /locus_tag="PLK68_04895"
     CDS             1970..2362
                     /locus_tag="PLK68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRM89783.1"
                     /translation="MNEVYEFLKKCGTYYLATVEGYKPRVRPFGTIDLYNNRLYIQTG
                     KVKAVSRQIKENPQIEICAMDDGKWIRIETTAYLDNNIDAQKHMLAAYPSLQKMYQPG
                     DGNTEVFYLRNVTAKICSFTEEPKIFKF"
     gene            complement(2428..3453)
                     /gene="rfbB"
                     /locus_tag="PLK68_04900"
     CDS             complement(2428..3453)
                     /gene="rfbB"
                     /locus_tag="PLK68_04900"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRM89784.1"
                     /translation="MKILVTGGAGFIGGNFVHYMVETYPEDMIVNLDLLTYAGNLETC
                     KPVEGKPNYKFVKGDIADREFIFKLFEEEKFDVVVNFAAESHVDRSITDPEIFVKTNV
                     MGTTTLLDAAKEFGVKRYHQVSTDEVYGDLPLDRPDLFFTETTPLHTSSPYSSSKASA
                     DLFVLAYHRTFGLPVTISRCSNNYGPYHFPEKLIPLMISRALADEELPVYGKGDNVRD
                     WLHVYDHCVAIDLIIRKGRVGEVYNVGGHNERTNLEVVQTILKALNKPESLIKYVEDR
                     KGHDRRYAIDPTKLETELGWKPKYNFDTGIQQTIQWYLDNKEWWQNILSGEYQNYFEK
                     MYAGKVK"
     gene            complement(3454..4029)
                     /gene="rfbC"
                     /locus_tag="PLK68_04905"
     CDS             complement(3454..4029)
                     /gene="rfbC"
                     /locus_tag="PLK68_04905"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRM89785.1"
                     /translation="MKKIETKIPGVVIIEPDIHGDHRGYFMETYSTSNFHELGIDNVF
                     VQDNMSFTAKKGTLRGLHFQNDPMAQAKLVSCTKGTVIDVAVDIRKGSPTYKQWVAVE
                     LSEENKKMFFIPRGFAHGFLTLTDNVEFRYKVDNLYSKEHDRGIRYDDPSVNVDWGGL
                     LNGIEPILSDKDKNGPTLDESDCNFKFEGDK"
     gene            complement(4041..>4825)
                     /gene="rfbA"
                     /locus_tag="PLK68_04910"
     CDS             complement(4041..>4825)
                     /gene="rfbA"
                     /locus_tag="PLK68_04910"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRM89786.1"
                     /translation="IYYPLSILMEAGIKDILIISTPDDTPRFEELLGDGSQFGISLQY
                     KVQPSPDGLAQAFILGEEFIDGEGCAMVLGDNIFHGHGLGKRLREAANKFSGATVFGY
                     YVDDPERFGVVEFDENGKAVSLEEKPANPKSNYAVTGLYFYDKNVVEFAKSIKPSARG
                     ELEITDLNKIYLDNGTLDVTLLGQGFTWLDTGTHESLVDATNFVKTVETHQNRKIACL
                     EEIAYNNGWISKDQLNTSYELYKKNQYGKYLKDVLDGKFIDQ"
BASE COUNT         1723 a          709 c          792 g         1601 t
ORIGIN      
        1 acaatccctt ataaaaaatc aattcaaaat attcgtaaag taattgataa aattgaagct
       61 ggtaaaaaac ttactgaagc tgatgtaaaa ggattaagtt agccgacgat tatagtcggc
      121 tttttatgtt aaatttgtta aatttattta agattgttga tttttttgtt aattaagcga
      181 attgattgaa accgatatta tttgtgctat aatgagataa aatttaaggt ttgttgttgt
      241 aaggagagaa gtagattggc gtttattaag aagataaaaa aattaaaaaa gagtgttaaa
      301 attactggtg gagtaattat aggagttatt gttgtctttg ctattttttc attttattat
      361 cgtgataagt ggtatcctaa tacatctatt aatggaatcg atgtatcaag catgacttat
      421 gaagaaagta aaaatgtatt aaagaaccag attgaaagtt atgctttgga aataggggca
      481 gagggaaatc aaaagcttac gattgcaggg aaagatatta atttaagtgc agattttgaa
      541 aaaaatctag aaagtgacta taaaaatcat cgcagtcaac gtagtatttt tgggattttt
      601 agtggttaca accatgatat tgatttaaaa atatcttatg atcaaaataa attaagtgaa
      661 atagttaatg gttcagtgct tatcaatggg aatgaagaat atcaaattgt gcagtcaaca
      721 aatgctcata ttgaatacga tgaaacaacg aagagtggaa aaatggttaa agcaacaatt
      781 ggaaatgaac taaatctcga aaaatttagt aacttaatta caacgtcaat aagtaaatta
      841 actacaaaga ttgatttaac tgatcaagat aaatatgcag aagtttatca acagccagtt
      901 agtgatatta gtgataagca tttagaagag atgttgaata cgtataataa ttatctatta
      961 aattggatca actgggatat gggagaaggc aaagttgaga cgatgacacc agatgatatt
     1021 aaaaactggt taagctgtaa tgataaaggt gaagttgtcc ttgataaaga agcaatgagt
     1081 gagtggattg aagaattttg tttgcgctat aagacagttg gaaagaaacg taattttacg
     1141 actcataatg gaaacgtgat tcagatttca ggtggtgact atggctggcg ccttgattat
     1201 gaaaagatcg ttaaacaagt agaagcggcg attactgaaa aaacggacag taaattaatt
     1261 gaggcctacc tcagtgagca aagtaaaaag aatcaaaaag ctttaacaac ggaattagaa
     1321 ccaacttatt ctaataaggc atatcaaaaa gattatgaaa actttgaaaa tgattgggat
     1381 actcaaaatt atagtgaaat tgatttaact gaacaaagag tatacgtgta tcgtgatggt
     1441 caattagctt attcttgtat ttgtgtaagt ggattaccaa ctgagaaaaa tgatcgtatc
     1501 acacgtacag gggtatggta tatcaaagag aaaaaaccag agaaagtttt agttggcgaa
     1561 gattatgaga ctccggttaa atattggatt agaattatgt ggacaggaac tggatatcat
     1621 gctttggatc gaagcgactg ggcaaattgg actccagatc tgtataaagt aaaaggatca
     1681 catggatgtt tgaatcttca agaagaggat gccaaaaaat tatatgagct gattagaatg
     1741 aatgatccag tttttattca ttattaaaat tattaaaaag cattttctta gttaaactaa
     1801 aggaatgctt ttttaatttt aattatttta aattatttat tataaatgct gataagagag
     1861 attaaagaat aaaattataa ctaatataat gatttaacta agtttataat gattagattg
     1921 tactaatgag ttgaggggag taaaatatat attataagga ggagctggta tgaatgaagt
     1981 ttatgaattc ttgaagaaat gtggaactta ttatttagct actgttgagg gttataaacc
     2041 gcgggttagg ccatttggga caattgattt gtataataat agattgtata tccaaactgg
     2101 taaagttaaa gctgtttcgc gacaaattaa agagaatcct caaatagaga tttgtgctat
     2161 ggatgatgga aaatggatta gaattgaaac aactgcttat cttgataata atattgatgc
     2221 acaaaaacat atgttagcag cctatccatc attgcaaaaa atgtatcagc ctggagatgg
     2281 aaatacagaa gtattttatc ttcgtaatgt aactgcaaaa atctgttctt ttacagaaga
     2341 accaaaaatc ttcaaatttt aattaatttg ataataaaaa aattgttgaa ctcatttatg
     2401 ttcaacaatt tttaatttaa ataacaatta ttttactttt ccagcgtaca ttttctcaaa
     2461 atagttttga tattcaccag aaagaatatt ttgccaccat tctttattat ctaaatacca
     2521 ttgaatggtt tgttggatac cagtatcaaa gttatatttt ggtttccatc caagttctgt
     2581 ttctaattta gttggatcaa tagcataacg acgatcatgc cccttacgat cttcaacata
     2641 tttgattaaa gattcaggtt tatttaaagc ttttaaaata gtttgaacaa cttctaaatt
     2701 agttctttca ttatgaccac caacattata aacttcacca acacgacctt ttctaataat
     2761 taagtcaatt gcaacacagt gatcgtatac atgtaaccag tcacgaacat tatcaccttt
     2821 accatatact ggtaattctt catcagctaa agctcttgaa atcattaatg gaattaactt
     2881 ttcagggaaa tgatatggac catagttatt tgaacatcta gaaatagtta ctggtaatcc
     2941 aaaagtacga tgataagcta aaacaaataa atctgcagaa gcttttgatg aactataagg
     3001 actagatgtg tgtaatggtg ttgtttcagt aaagaacaaa tcaggtcgat ctaaaggtag
     3061 gtcaccatat acttcatcag tagatacttg atgatatctt tttactccaa attctttagc
     3121 tgcatctaat aaagtagttg ttcccataac atttgtttta acgaagattt caggatcagt
     3181 aatacttcta tcaacatgtg attctgctgc gaagttaaca acaacatcaa atttttcttc
     3241 ttcaaataac ttaaagatga attcacggtc agcaatatca cctttaacaa atttataatt
     3301 cggtttacct tcaactggtt tacaagtctc taagtttcca gcataagtta ataaatctaa
     3361 gttaacgatc atatcttctg gataagtttc aaccatatag tgaacaaagt ttccaccgat
     3421 gaaacctgct ccacctgtta ctaaaatttt catttattta tctccttcga atttaaaatt
     3481 acaatcactt tcatctaatg taggaccatt tttatcttta tctgataaga ttggttcaat
     3541 tccatttaat aatccgcccc aatcaacatt gaccgaagga tcatcgtaac gaattccccg
     3601 atcatgttct ttagagtata aattatctac tttgtatcta aattcaacat tatcagttaa
     3661 tgttaagaaa ccatgagcaa atcctcgagg aataaagaac atcttcttat tttcttcact
     3721 caactctact gcaacccatt gtttataagt tggagaacct ttacgaatat caacagcgac
     3781 atcaataaca gttcctttag tacagctaac taatttagct tgggccattg gatcattttg
     3841 gaaatgcaag cctcttaaag tccctttttt tgctgtaaaa gacatgttgt cttgaacaaa
     3901 gacattatca attcctaatt catgaaaatt acttgttgaa tatgtttcca tgaaataacc
     3961 gcggtgatca ccatgaatat ctggctcaat gataaccaca ccagggattt tagtttcaat
     4021 ttttttcatt tgtctacctc ttattgatct ataaatttac cgtctaatac atcttttaaa
     4081 tatttaccat attggttttt cttatacaac tcatatgatg tattaagctg gtctttggaa
     4141 atccacccgt tattataagc gatttcttca agacatgcaa tcttccggtt ttgatgagtt
     4201 tctacagttt taacaaagtt agttgcatcg actaaacttt catgagttcc tgtatctaac
     4261 caggtaaatc cttgtcctaa taaagttaca tctaaagtac cattatctag atagatttta
     4321 tttaaatcag taatttctaa ttcgccacgg gcacttggtt taatactttt agcgaattca
     4381 acaacatttt tatcatagaa atatagccct gtaacagcat aattagattt aggattagct
     4441 ggtttttctt ctagtgaaac agctttacca ttttcatcaa attcaacaac cccaaatctc
     4501 tcaggatcat caacataata tccaaatacg gttgctccac taaacttatt agcagcttct
     4561 cttaaacgtt tacctaagcc atgaccatgg aaaatattat cccctaagac cattgcacat
     4621 ccttcaccat caataaattc ttcaccaaga ataaatgcct gagctaatcc atcaggagag
     4681 ggttgtactt tgtattgaag tgaaatccca aattggctgc cgtcacctaa tagttcttca
     4741 aaacgaggag tatcatcagg tgtcgagatg attaggatat ctttgattcc agcttccatc
     4801 aaaatactta atggataata aatca
//
LOCUS       DAOMTA010000132         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_447_length_4810_cov_2.611987, whole genome shotgun sequence.
ACCESSION   DAOMTA010000132 DAOMTA010000000
VERSION     DAOMTA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_4810_cov_2.611987"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2417
                     /locus_tag="PLK68_04915"
     CDS             156..2417
                     /locus_tag="PLK68_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM89787.1"
                     /translation="MRDPDKKKTISNEVIFIVIGIVGVILLVPIFITFVFWYLDTNVL
                     QQVDENFLNTFVSHSKEILIISVSLYALAFYYVVTFFRTVKPKKTDMLASQCWSTVSE
                     QKKFFGSTVVDASLNLEEGGSPINYLPNENIILYEKGAVHDIVVGTTRCGKSRKIVRQ
                     LVMIVSMAGESMIFNDPKKEMYFDFRNFLEKKKDYKVNCLDFRNLQYSDGWNPLDSII
                     DCLQDRDDPDIDNADQYSQDMVTSIVVDSGKGEQIWIDGQKALIKGIILANCQANVSE
                     DKKNFYSVYQTLALLGGEQAYNNNPNDKKMKLSAYMNSLDETDIARTAYTTITNSPEK
                     TRGSFMTSALATLQLFSSIKLMKVLSKSDFSFREFTEGKRALFIVNPDEKKTYDRIAG
                     ICFDQSYQTLVFEANRMQGRRLKKKVHMIYDEFGNMPTIDAMQSKMTVALSRGIVYHL
                     YVQGFDQLNDRYDESIARIIRGNCNLTYFISSADLNTCKEVAESIGDETIWGPSHSAN
                     YNPIASSTGSGVNYSMSKRQLVDANELMVADNRDGNGIIVKRTYFSPSKVYLPDCSEY
                     PWYEEMKTDETEISKADRELNWAVPRCFELRETEIRQAVYTNEDCNSISMSERRRPIL
                     TQKNSMPNEKELYWYWSMRNDLSESVKKHVIQHIANCNKILSRKEIMKYLKSKEFFDY
                     INPIDVADKQRHSTESADRVKMVKTSLQSFDNNSSQKQSTLMKKIDDLSIPQLRELAS
                     VARVIRDGKERNG"
     gene            2410..3714
                     /gene="mobL"
                     /locus_tag="PLK68_04920"
     CDS             2410..3714
                     /gene="mobL"
                     /locus_tag="PLK68_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HRM89788.1"
                     /translation="MAEIIFNAEFVKTEYAGNSCKNIKKQINTDEMVDYYERKEACDY
                     TVDENEADDTYQINSQDAFNYYDYRIGSTGGFNRNGNLEKGEANRLMEKYQPKILWRF
                     VFSFEKEFAQNSNIITKTNMQKLMTKSMEKNIRALGFDPQNVEWGAYYHTNTDNPHCH
                     VWMFEKEPTKRYYKISKKKFNKVRSNVIRTMALNSEIYIERDNLKEELLNCIKNDELI
                     KLFKSSKNNSKKCFKKNNGIINLLLDLNDKLPLNGSLKYNSKNIRPYHDDIDKIVDII
                     LNTNNVKLFYCQYKKHLQKEKEIFNNRYFSDEEKMEQNRSFENKDKELRTRIANMVLQ
                     NIKNYRLDNEHYSKLENKIKKETIKTSDKKHSYINKFSAVNASKHSMSCRSSLLQAGV
                     LDELSKAIMESSNASSIIEKELDDMVKKAQKEIRANSFERSL"
     gene            3777..4019
                     /locus_tag="PLK68_04925"
     CDS             3777..4019
                     /locus_tag="PLK68_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89789.1"
                     /translation="MDKQIRELNKKIAALEKKINDAQNQIDRMEKIKKEAKEELTPLN
                     RVKEKLTAIQNELENTIQKKEVTNDNEETDRSLQRY"
     gene            3979..4584
                     /locus_tag="PLK68_04930"
     CDS             3979..4584
                     /locus_tag="PLK68_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRM89790.1"
                     /translation="MTMKKLIEVYNGINKTYYCRYDLNAFGLSFKKTGKYSGKWVGKA
                     DEDKANAIKEYCIKNKLRVNITDLAYTRAHNYREVYFENNKGIFGDGRYYHCVYCGKI
                     LKKDKVTVDHFFPINKVKNSPYSSINIRLLKKFGIEDINDKRNLVCACKSCNSSKGSK
                     GGIWLVRGYLGRFFILWVLFYTLLLYFIGYYLIYAFNCFIK"
     gene            4598..>4810
                     /locus_tag="PLK68_04935"
     CDS             4598..>4810
                     /locus_tag="PLK68_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89791.1"
                     /translation="MKINGVTIVNFKSDNNLYQCDVIFDGKFLGCYKQKSSYSDYCSF
                     DKKLLYESMVKFKESKLYKNNCFVTDD"
BASE COUNT         1873 a          582 c          916 g         1439 t
ORIGIN      
        1 ccaaaagtga atattcctac ttatttttga ttcagtattc attcaaaaaa tgatttctaa
       61 atcatttttc aagaatattc actttttgtc ggacgaattt atcatattga ggcattatgc
      121 ctctttttat ttattcaaag aaaggggatg tgtaaatgag agatcctgat aaaaaaaaga
      181 ctatctcaaa tgaagtgata ttcattgtga taggaattgt tggtgttatt ttgttagtgc
      241 cgatttttat aacgtttgta ttctggtatc tggatacaaa tgtgcttcaa caggttgatg
      301 aaaatttttt aaataccttt gtatcccatt caaaagaaat attaattatc agtgtaagtt
      361 tatatgcatt ggcattttat tacgtagtga cttttttcag aacggtaaaa ccaaaaaaga
      421 cggatatgct tgcaagtcag tgttggtcta ctgttagtga gcagaaaaaa ttttttggtt
      481 caaccgtggt ggatgcaagt cttaatcttg aggagggagg ttcaccgata aattatctac
      541 ctaatgaaaa cataatccta tatgaaaagg gagctgtaca tgatattgta gttggaacaa
      601 cacgatgcgg aaaatctaga aagattgttc gccagcttgt aatgatagtt tccatggcag
      661 gtgaatcaat gatatttaat gaccctaaaa aagaaatgta ttttgatttt agaaattttc
      721 tggagaagaa aaaagattat aaagttaact gtcttgattt tagaaattta caatacagcg
      781 acggatggaa tccgttagat tcaataattg actgtttaca ggatagagat gatccagata
      841 ttgataatgc agatcagtac agtcaggata tggtaacatc aatcgttgtt gatagtggga
      901 aaggtgaaca aatctggata gacggtcaaa aggcacttat taaaggaatt atattagcaa
      961 actgtcaggc taatgtatct gaagataaaa agaattttta ttctgtttat cagacgcttg
     1021 ctcttcttgg aggtgagcag gcatacaaca ataatcctaa cgacaagaag atgaaacttt
     1081 ctgcatatat gaattcactt gatgagacag atatagcaag aacagcttat acgacaatta
     1141 ctaattcacc tgaaaagacg agaggttctt ttatgacaag tgcattggca actttacagc
     1201 tcttttcgag tattaaactg atgaaggtac ttagtaagag tgatttcagt ttcagagaat
     1261 ttacagaggg caagcgtgct ttgtttattg ttaatcctga tgagaaaaaa acttatgata
     1321 gaattgcagg tatatgtttt gaccagtcat atcaaactct agtctttgaa gctaatagaa
     1381 tgcagggaag aagattgaaa aaaaaggtac acatgattta tgatgaattt gggaatatgc
     1441 ctacaattga tgcgatgcag agcaaaatga ctgtagccct ttctagagga attgtatacc
     1501 acttgtatgt gcagggattt gatcagctga atgatagata cgatgaatcg attgcaagaa
     1561 ttattcgggg aaactgtaac cttacttatt ttatctcaag tgcagatttg aatacatgca
     1621 aggaagttgc tgagagtatt ggtgatgaaa caatctgggg tccaagccat agtgccaatt
     1681 ataacccgat tgcttcttct actggcagtg gtgtgaacta ttcaatgtct aaaagacagc
     1741 ttgttgatgc aaatgaatta atggtggcgg acaatcgtga tggaaatggt attattgtta
     1801 agaggactta cttttctcct tcaaaagtat atttacctga ctgctcagag tatccatggt
     1861 acgaagaaat gaaaactgat gaaacggaaa ttagtaaagc agacagggaa ctaaactggg
     1921 cagtcccgag atgttttgaa ttgcgagaaa ctgaaataag acaagcggtt tatacaaatg
     1981 aagattgtaa ttcaattagt atgagtgaaa gaaggagacc aatactgact caaaaaaatt
     2041 caatgcctaa tgaaaaagaa ctatactggt actggtctat gagaaatgat ctgtctgaaa
     2101 gtgtaaaaaa gcatgttatt cagcatattg ccaactgcaa caaaatacta tcaagaaaag
     2161 aaataatgaa atatctaaaa tcaaaagaat tttttgatta tataaatcca atagatgtag
     2221 cagataaaca gaggcattca actgaatcgg ctgatagagt aaaaatggta aagacatcat
     2281 tgcagtcatt tgataataac tcatcccaaa agcagtctac gttaatgaag aaaatagatg
     2341 atttatcaat accgcagtta agagaactag caagtgttgc tagagtaatt agggacggta
     2401 aagaaagaaa tggctgaaat tatttttaat gctgaatttg ttaaaacaga atatgcaggc
     2461 aacagctgta aaaatattaa aaaacaaatt aatactgatg agatggttga ttactatgag
     2521 cgaaaagaag catgtgatta tactgttgat gaaaacgaag ctgatgatac atatcagata
     2581 aattcacaag atgctttcaa ttattatgat tatcgaatcg ggtctactgg tggatttaat
     2641 agaaatggta atcttgaaaa aggagaggcg aacaggctga tggaaaaata tcagccaaaa
     2701 atattatggc gctttgtgtt ttctttcgaa aaggaatttg cccagaacag taatataatt
     2761 actaagacta atatgcagaa attaatgact aaatctatgg agaaaaatat tcgtgcatta
     2821 ggttttgatc cgcaaaatgt cgaatggggc gcatattatc ataccaatac agataatccg
     2881 cactgtcatg tgtggatgtt tgaaaaagaa ccaacaaaac gttattataa aatctcaaaa
     2941 aagaaattta ataaagtacg gtcaaatgtt attagaacaa tggcacttaa tagcgaaatt
     3001 tatatcgaac gagataattt aaaagaggaa ttattaaatt gtattaaaaa tgatgaatta
     3061 ataaaattat ttaagtcttc aaaaaataat tcaaaaaagt gttttaagaa aaataacgga
     3121 attatcaatc ttcttttaga tctcaatgat aaattaccgc taaacgggag tttaaaatat
     3181 aattcaaaaa acattcgacc atatcatgat gatattgata aaattgttga cataatttta
     3241 aatacaaata atgttaaatt attttactgt caatataaaa aacatcttca aaaagaaaaa
     3301 gaaatattta ataaccgtta ttttagtgat gaggagaaga tggaacagaa ccgctccttc
     3361 gagaataagg ataaggagct gaggactaga atagccaata tggtattgca gaatattaaa
     3421 aattatcgtt tggataatga acattattct aaattggaaa ataaaatcaa aaaggaaaca
     3481 atcaaaacga gtgataagaa acattcgtat ataaataaat ttagtgctgt taacgcatcg
     3541 aagcattcta tgagttgtcg aagttctctg ttgcaggcag gtgttttaga tgaattatca
     3601 aaagcaataa tggaaagcag taatgcttca agcataattg aaaaagaatt agacgatatg
     3661 gtaaaaaaag cacagaaaga aattagggct aattcatttg agcgctcatt ataaaaataa
     3721 atcatataaa agatgcagtt tgcctctttg atattttaag aaaggatgaa taagttatgg
     3781 acaaacaaat tagagaatta aataaaaaaa tagcagcgtt ggagaaaaaa attaatgatg
     3841 cacaaaatca aatagatagg atggagaaaa taaagaaaga agcaaaagag gaattaacac
     3901 cattaaacag agttaaagaa aagttgacag caattcaaaa tgaacttgaa aatactatcc
     3961 aaaaaaaaga agtgactaat gacaatgaag aaactgatag aagtctacaa cggtattaat
     4021 aaaacatatt actgcaggta cgatttaaat gcatttgggt tatcttttaa aaaaacagga
     4081 aaatattctg gtaagtgggt cggcaaggca gatgaagata aagcaaatgc aatcaaagag
     4141 tactgtataa aaaataaact gagagtaaat ataacagatc tggcgtacac aagggctcat
     4201 aactacagag aagtatattt tgagaacaat aaaggaattt ttggtgatgg aagatattat
     4261 cattgtgtat attgtggaaa aatcttaaaa aaagataaag ttacagtaga tcactttttt
     4321 cctatcaata aagttaaaaa cagtccgtat tccagtatta acataagact actaaaaaaa
     4381 tttggaatcg aagatattaa tgataaaaga aacctcgtat gtgcctgcaa gagttgtaac
     4441 tcgtcaaaag gatcaaaagg tggaatttgg cttgtgagag gctatcttgg aagatttttt
     4501 atcttatggg tattatttta tacattactt ctatatttta taggatatta tttaatatat
     4561 gcttttaatt gttttattaa gtagtggggt aaaaatcatg aaaataaatg gtgttacaat
     4621 agtgaatttt aaaagtgata ataatctcta tcaatgcgat gtaatatttg atggaaaatt
     4681 tttaggatgt tataaacaga aaagcagtta tagtgattat tgttcttttg ataaaaaatt
     4741 actgtatgaa agcatggtaa aatttaagga gagtaaactt tataaaaata attgtttcgt
     4801 tacagatgat
//
LOCUS       DAOMTA010000133         4797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_450_length_4797_cov_2.481442, whole genome shotgun sequence.
ACCESSION   DAOMTA010000133 DAOMTA010000000
VERSION     DAOMTA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_4797_cov_2.481442"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..937
                     /locus_tag="PLK68_04940"
     CDS             164..937
                     /locus_tag="PLK68_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaA family SipW-dependent biofilm matrix
                     protein"
                     /protein_id="HRM89792.1"
                     /translation="MKNKKSVVASIVLSGALVVVGTLAYFTQTHTVDNKLKTKGFGSD
                     IVEKFTPKEFNPGATVTKEVRVDNTGDYALVARAKWEESWTRNGEEFKAVAYPDTNNE
                     SVVDKNGMSDKWVDGNDGWAYYNEMIGVNGHTENFLTSITLKNSADVVGTDIKNFYYT
                     TAATEPDKTSIGTDSKTQWVKISEEEFKALDDENNNIKATFKRAEVKSNGLYDNAEYT
                     LTITAQVSQANKEAAATWITDATNQTVKNFLNGLPTVNN"
     gene            1170..1898
                     /locus_tag="PLK68_04945"
     CDS             1170..1898
                     /locus_tag="PLK68_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaA family SipW-dependent biofilm matrix
                     protein"
                     /protein_id="HRM89793.1"
                     /translation="MKRKKSLIAIISLSAFLVIGGAVAYYNSTTNVDNKLKTGQFKSE
                     VVEKFTPKDNWEPGDKVTKEVGVENTGTGNIVSRAMWSETWKTADGEDITVTSADPRA
                     SVVEKEYGNKWIYSNSDGYFYYDGIISSGGRAEDKFLKSIMLSGDVDFAETKKTVVYY
                     STMTTGEPSKITNNPETGWAVAPNTGIPNNATYNKTVVTGNGKYASANYTLTITVQVY
                     QANKQAVAGTAFETVVPETYALLD"
     gene            1919..2695
                     /locus_tag="PLK68_04950"
     CDS             1919..2695
                     /locus_tag="PLK68_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaA family SipW-dependent biofilm matrix
                     protein"
                     /protein_id="HRM89794.1"
                     /translation="MKNKKSLSALISLAAIVLVVGTLAYFSRDLTVENRLKTAKYDTT
                     IEEEFIPTDKWAPGVEINKKVTVKNTGNVDIVVRAKLTELWKRSENLMDPNDPDKILS
                     EEGEILPNSFVDENGISHDAAIKNFVQNMVYEYEDVKDNLADYHNKWIHYGDYYYYLG
                     VIGEDEASNGLLNSVKMNPLLDATVSGSHTVVEADEQGTTTVTKKYQYGKYGYDSADY
                     TLTVEARTVQASKAAIRDAFGDNVMAVYLADHFATIAEQQ"
     gene            2718..3431
                     /locus_tag="PLK68_04955"
     CDS             2718..3431
                     /locus_tag="PLK68_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaA family SipW-dependent biofilm matrix
                     protein"
                     /protein_id="HRM89795.1"
                     /translation="MKNKKSLGGILLLALTVGVVGSLAYFSQELTKTNEFKTAKYDTT
                     IEEEFTPPTDWLPGVEVNKDVTVKNSGNVDVVVKATLTESWTRGDETLSNTFTDENGL
                     TQNAALLALPNVVKYTDDIELAAQAGKWVEYEGTYYYMGAIKGGNSSALLLDNVTLNP
                     LLDVTDKSVTTVVTTDENGTKKEITTTEKGKYGYDDANYKLTVTANTIQATASAIKTW
                     GSNPVVDYIVANYATIAEK"
     assembly_gap    3644..3679
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3680..4637
                     /locus_tag="PLK68_04960"
     CDS             <3680..4637
                     /locus_tag="PLK68_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89796.1"
                     /translation="TPIVEDKYNEVAIGNLVWALMPLSNDELERIEESHRIRPYLVVA
                     KDENYFYGYYCSTKQKARYLATFKLDKNIYDHRKNTYVYLTNTYKIPRTNFRDIYNRI
                     GINNLIMIERKLIVNSRYLEGLIHFDVPIIYCVGDIIRVGDQLYYIYQTDISNLYVNK
                     TKFVREAALRYEFDYSETIIFDCKKSYELVNMLSPKHIEIVYENKRKYKHEQKRKQVK
                     QYKNVFNYPRGSVFEDCNGDKIIYLYSRSNHHYGINTKINEFFPYICDIQNIEKANII
                     GTINDGKMMIMLERLLDQNINPHNIVTMIYQEIMDERPRSRV"
BASE COUNT         1838 a          565 c          921 g         1437 t
ORIGIN      
        1 accggaaaaa aaccaaataa agatatactg ttaattgtaa aagaaaagtt ttcttcgata
       61 gttatattaa tagaataaga gttataaata taaccagaag caaaccatca attgttatca
      121 ttgatttgta aaatgaaata aaaaataaaa aggagtggaa atcatgaaaa acaagaaaag
      181 cgtagtggca tcaattgtat tatcaggggc attggtagtt gtaggaactc tagcatattt
      241 tactcaaaca catacagttg acaataaatt aaaaacaaaa gggtttggct ctgatattgt
      301 cgaaaaattt acacctaaag aatttaatcc aggagcaact gttacaaaag aggtacgagt
      361 tgataataca ggagattatg ctttagtagc acgtgctaaa tgggaagaat catggacacg
      421 caacggagaa gaatttaaag cagtagctta tccagacact aataatgaaa gtgttgttga
      481 taaaaatggt atgagcgata aatgggttga cggtaatgat ggatgggcat attataatga
      541 gatgattgga gtaaatggac atacagaaaa tttcctaact tcaattactt taaaaaatag
      601 tgctgatgta gttggaacag atattaaaaa cttttattat acaactgcag caactgaacc
      661 ggataaaaca tcaattggaa ctgactcaaa aactcaatgg gtaaaaattt ctgaagaaga
      721 atttaaagca ttagatgatg aaaataacaa tattaaagcg acatttaaac gtgctgaagt
      781 taaatcaaat ggtttatatg ataacgctga gtatacatta acaattactg ctcaagtttc
      841 acaagctaat aaagaagctg ctgcaacttg gatcactgat gcaacaaatc aaactgtaaa
      901 aaatttcttg aatggattac ctacagtaaa caactaatga agccacgaac actctaacaa
      961 ttgctagagt gtttttcttt ataagttaag acaattattg atttttagat tcaataaaaa
     1021 cattaaaata gaatattaag gaaagtgaaa tataaccaga agcaaaccat gattatttat
     1081 aataatgttg taatatgaca aaagtatatt tgttggttat taataccaca tatcggttat
     1141 taaatatttg agaaaggggt acgaaaatta tgaaacgaaa aaagagtttg attgcaatta
     1201 tttcgttatc agcattttta gttataggtg gggcagttgc ttattataat agcacaacga
     1261 atgttgataa caaattaaaa actggacagt ttaagtcaga agttgtggaa aaatttacac
     1321 caaaagataa ttgggaaccc ggagataaag ttactaaaga agtaggtgtt gaaaatactg
     1381 gaactggtaa tattgtttct cgagcaatgt ggagtgaaac atggaaaaca gctgatggtg
     1441 aagatattac agttacaagt gctgatccaa gagctagtgt tgttgaaaaa gaatatggta
     1501 ataaatggat atattcaaat agtgatggat atttctatta tgacggaata atttcttcgg
     1561 gtggtagagc cgaggataag tttttaaaat caatcatgtt aagtggagat gtcgattttg
     1621 ctgaaactaa gaaaacagta gtatattatt cgacgatgac aactggtgaa ccaagcaaaa
     1681 taactaataa tcctgaaaca ggatgggcag ttgccccaaa tacaggcatt cccaataatg
     1741 caacttacaa taaaacagtt gtaactggta atggtaagta tgctagtgcc aactatactt
     1801 taacgattac agttcaagtt tatcaagcta ataaacaagc agtcgcagga accgcttttg
     1861 aaactgtagt tcctgaaacg tatgcattat tagattaaaa agaaagtgag tgatagaaat
     1921 gaaaaataag aaaagcctat ctgcccttat ttcattagct gcaattgtat tggttgtagg
     1981 tacattggct tacttttcta gagacttaac tgttgaaaat agacttaaga cggcaaagta
     2041 tgatacaacg atcgaagaag aatttattcc aactgataag tgggcaccag gtgttgaaat
     2101 taataaaaaa gttacagtta aaaatactgg aaacgttgat attgtggtaa gagcaaaatt
     2161 aactgagcta tggaaacgta gtgaaaatct gatggatcct aatgatccag ataaaatctt
     2221 gagcgaggaa ggtgaaattt taccaaattc attcgttgat gaaaacggca tttcacatga
     2281 tgcggcaatt aaaaattttg ttcaaaatat ggtttatgaa tatgaagatg ttaaagataa
     2341 tctagcagat tatcataata aatggattca ttatggtgat tattactact atttaggtgt
     2401 aattggtgaa gatgaggcat caaatggatt attaaatagt gtgaagatga atccgttatt
     2461 agatgcaaca gttagcggca gtcatacagt cgttgaagca gatgaacaag ggactacaac
     2521 tgtaactaaa aaatatcaat acggaaaata tggatatgat agcgcagatt atactttgac
     2581 agttgaagcg cggacagtcc aagcttcaaa agcagcaatt agggatgcgt ttggtgataa
     2641 tgtgatggct gtgtatttag ccgatcattt tgctacgatt gctgaacaac aataaagaaa
     2701 aggaaaggtg aagaagaatg aaaaataaaa aaagtttagg tggaatatta ttattagcat
     2761 taacagtagg agtagttggg tcgttagcgt attttagtca ggaattgaca aaaacaaatg
     2821 aatttaaaac agcaaaatat gatactacaa ttgaagaaga atttactcca ccaactgatt
     2881 ggttaccagg agttgaagta aataaagatg taacagtaaa aaacagtggt aatgttgatg
     2941 tagttgttaa agcaacatta acagagtcat ggacccgcgg tgatgaaact ttaagcaata
     3001 catttactga tgaaaatggt ttaacacaaa atgcggcttt attggcattg ccaaatgttg
     3061 tgaaatatac tgatgatata gagttggcgg cacaagcagg taaatgggtt gaatatgaag
     3121 gaacttatta ttatatggga gcaatcaaag gcggtaatag ttcagcttta ttattagata
     3181 acgttacttt aaatccacta ttagatgtaa ctgataagag tgttactacg gttgtaacaa
     3241 ctgatgaaaa tggaacaaaa aaagaaatta caactaccga aaaaggtaag tacggatatg
     3301 atgatgctaa ttataaatta acagtaactg caaatacgat tcaagcaaca gcttcagcta
     3361 ttaaaacttg gggaagtaat ccagttgtag attacattgt tgctaattat gcaacaattg
     3421 ctgaaaaata agatataaat atagaaagaa cgaactgtac ccctcacttc agttcgtttt
     3481 ttcaattata taatgagtaa caaaaagatt ataggattaa tgcttctata atctttttgt
     3541 attctttgat tgttgggtat ataattatct tggggtggga agatgaaatt gtttaaaaga
     3601 ttattaaagt attttagtag actagaaaaa gaggaaatta tagnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnt acgccaattg ttgaagataa atataatgaa gtggcgatag
     3721 gaaatctagt atgggctttg atgccgttaa gtaatgatga gttagaacgt atagaagaaa
     3781 gtcatcgaat taggccctat ttggtcgttg ctaaggacga aaattatttt tatgggtatt
     3841 attgttctac aaaacaaaag gcccgttatt tagctacatt taagcttgat aagaatatat
     3901 atgaccatcg taaaaataca tatgtctatt taacaaacac ttataagatt ccgcgaacta
     3961 attttcgaga tatttacaat cggattggaa ttaataattt aataatgatt gagcgaaaac
     4021 tgattgttaa ttcacgctac ctagagggat tgattcattt tgatgttccg attatttatt
     4081 gcgttggtga tattattaga gtgggtgatc aattatatta tatttatcaa acagatattt
     4141 caaacttata tgttaataag actaagtttg ttcgtgaagc agcattacga tatgaatttg
     4201 attacagtga aacgattata tttgattgta aaaaatctta tgagctggtt aatatgctat
     4261 caccaaaaca tattgaaata gtatatgaaa ataaacgaaa atataagcat gaacaaaaaa
     4321 gaaagcaagt taagcagtat aaaaatgttt ttaattatcc tcgcggaagt gtttttgagg
     4381 attgtaatgg tgataagata atttatttat atagccgttc taatcatcat tatggaatca
     4441 atacaaaaat caatgaattt tttccatata tatgtgatat tcaaaatatt gaaaaagcga
     4501 atattatcgg tactattaat gatggaaaaa tgatgatcat gctggaacgt ctgttagacc
     4561 aaaatattaa tccacataat attgttacaa tgatttatca agagataatg gatgagcgtc
     4621 ctagaagcag agtgtagaaa tctgcttttt ttattttttt tgcttctaag taggttttta
     4681 tgaataattg ctttataata taaacataag attttataga gaaagtaatg gtgataatat
     4741 gtatgttgca acaagtagac aaatgaaggc ttacgatcag gctttattaa atgaggg
//
LOCUS       DAOMTA010000134         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_453_length_4786_cov_2.625238, whole genome shotgun sequence.
ACCESSION   DAOMTA010000134 DAOMTA010000000
VERSION     DAOMTA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_453_length_4786_cov_2.625238"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..498)
                     /locus_tag="PLK68_04965"
     CDS             complement(127..498)
                     /locus_tag="PLK68_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89797.1"
                     /translation="MGQAKQRGTLEQRVKQAQEQKDDFYGTERSVAEVLAELGLPKES
                     TVKGYVIHLPEKDEFVATINDNNISFSVAYARTPELAMIYDEPQKAISDAKKISKHKL
                     LVCVLFETSNQYMINDIWANY"
     gene            679..1260
                     /locus_tag="PLK68_04970"
     CDS             679..1260
                     /locus_tag="PLK68_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89798.1"
                     /translation="MSITPRAVINMVKNTITSQENHVKHEADSSYLKKEKEQIYVQFQ
                     NERIEIWHNGEHQKGIEIKNQVDMKDAMAKAGEIIKEINGHLDNIVRQKQSPKVDKTF
                     ENEVTSKIGSNAVVVKQQPGKQDGIIIAENKDFVAHRLGENSKYVMFHSKASLDQVPP
                     VGAKVTITVPNDQNAKTKVVKSLDNSQTISKSR"
     gene            1417..1887
                     /locus_tag="PLK68_04975"
     CDS             1417..1887
                     /locus_tag="PLK68_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89799.1"
                     /translation="MIDNDCHDHNDIIHIKRQDNNQHSPSLGIPGTFGLIIFFGVCYL
                     ISQVWNAIENWQLYEAPAKYVLFIYHYMIYEPLAFGKIINDFLIVNTLTPFDNLNFVI
                     KWAFVILYYFGLFCVYLKYVSFLKNMGYKKYRFLIILSPAIFLMVYYFIEWLFS"
     gene            complement(2066..2587)
                     /locus_tag="PLK68_04980"
     CDS             complement(2066..2587)
                     /locus_tag="PLK68_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89800.1"
                     /translation="MQYNFTHELSELLHFTAGVGTAARLFSAYGSEFAHPQARPIDKV
                     YHLKKQSFLSRIFNKPQYLPQPTTDQKVFDRVVMSMESSQDLMWITDTIHNFNSLAEA
                     INSGNPENINKTCDRFITYFKWLMDHDQHTQKGSKGNPAETFSKDRFRGLIEIERAIN
                     AINSIKSKLLITE"
     gene            complement(2592..3404)
                     /locus_tag="PLK68_04985"
     CDS             complement(2592..3404)
                     /locus_tag="PLK68_04985"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRM89801.1"
                     /translation="MNKPTPQVYEALQHVYDTFNDRLFDGKLENSLITLQRKANSFGY
                     MSYNRFISIDDKNQFAHELALNPEFFGVKPLIELFQTICHELVHLYQMSYGKPSQKGY
                     HNKEFAAMMKERGLMASSTGRPGGNDTGQHMADYPIPGGQFLEVSNDLFNEGIIVAWY
                     DRFMPKNTSIHTMLEDQQIATDLTQGISESLLVIPHLPTEIDLSGDLDSIPLGATEPV
                     IQFVNRPLKPPKSKFKFTCPKCKNNLWGKESLNVICGDCSVSFELQINEYED"
     gene            complement(3420..3605)
                     /locus_tag="PLK68_04990"
     CDS             complement(3420..3605)
                     /locus_tag="PLK68_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89802.1"
                     /translation="MEHRKALKLMGKKISFIFNNETRIQHSFYWEVWGLLSEIGDHVV
                     SDEDLRKIKAMNAEFIG"
     gene            complement(3631..3873)
                     /locus_tag="PLK68_04995"
     CDS             complement(3631..3873)
                     /locus_tag="PLK68_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89803.1"
                     /translation="MALVIDNGHLLYDENDDRCKVFVKQSQGMLFGFGVFIDKGCPSE
                     TKKYWGKWDWNNQEKSLLSIMESGGKWDGWEVNNVN"
     gene            complement(3894..4310)
                     /locus_tag="PLK68_05000"
     CDS             complement(3894..4310)
                     /locus_tag="PLK68_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89804.1"
                     /translation="MTGPKLFQPTQQDLDTMKDKLLQQLNQEESFKQDAQAKGVPILK
                     RLVEVAKSDTGQSLVVRKFLLGCYDGQRFPFDLTDFRQLDLNLFQDCMAILAMDRSPT
                     REVHEYLDEPEYKGLVNGGALFEQWAKTVKNFSSTD"
BASE COUNT         1550 a          864 c          753 g         1619 t
ORIGIN      
        1 tgattaaatt tacatcattt tagtatgata cttttatagc tagtatgaca ctctattgtt
       61 tggtgtgata cttaattgtt tgcttacact atatgcaatt tttgacataa tagatttaaa
      121 ctcagactaa taattagccc aaatatcatt aatcatgtat tggtttgaag tttcaaataa
      181 aacacaaacc aatagcttat gttttgaaat ctttttagcg tctgaaatcg ctttttgtgg
      241 ttcatcatat atcatcgcta attcaggtgt ccgagcataa gcaactgaaa aagaaatatt
      301 attgtcatta attgtagcta caaattcatc tttttcaggt agatggataa cataaccttt
      361 tactgtactt tcttttggta agcctagttc agctagaact tctgctacac ttctttcagt
      421 tccataaaaa tcatcttttt gttcttgagc ttgctttact ctttgctcaa gtgtacccct
      481 ttgtttagct tgtcccattt attcaacctt ttggtttaca aatatattgt ttaagtttag
      541 aacattaatg tctagtttac gacctgattg tcaaactaca tccaactttc caatatgaaa
      601 ctttattgat tagtatgaca ctctattgtt tagtgtgatt cttaattgtt tgcttacata
      661 tgtaagaggg ttttagtaat gagtataacg cctagagctg taataaatat ggtgaagaac
      721 accatcacat cacaagaaaa tcatgtaaag catgaagccg acagttcgta tttaaaaaag
      781 gaaaaagaac agatttacgt tcaattccaa aatgaacgaa tcgagatatg gcataacggt
      841 gaacaccaaa aaggtatcga aataaagaac caggtagata tgaaagatgc tatggccaaa
      901 gctggtgaaa ttatcaaaga aattaatggt catttggata atatcgttag acagaagcaa
      961 agtcctaaag tggacaaaac ttttgaaaat gaggtaacaa gtaagattgg cagcaatgcg
     1021 gttgtagtga agcaacagcc aggaaaacag gacggtatca ttattgctga aaataaagat
     1081 tttgtggctc accgcctggg cgaaaatagt aaatacgtca tgttccattc aaaagcatca
     1141 ttggatcaag tgccaccagt gggcgcaaaa gtaacgatta cagtgcctaa tgatcaaaac
     1201 gccaaaacaa aagttgtgaa atccttagac aacagccaaa caatttcaaa aagtcgataa
     1261 ttccacaaaa cactatatat agtatcggtc gaaacaatct aaaccactat atatagtgtt
     1321 tataagcaac caatagtaaa gtgatattgc ctattttttg agtagtggta aaattatagt
     1381 attaatatgg cagcagccat gaagcggaga aacgccatga tagataatga ttgtcatgat
     1441 cataacgata taattcacat caaacgccag gataataacc agcatagtcc tagtcttggg
     1501 ataccaggta cattcggcct aatcatcttt tttggtgtct gttatcttat ttctcaagtt
     1561 tggaatgcta ttgagaactg gcagctatac gaagccccag ctaaatatgt acttttcatt
     1621 tatcactata tgatttacga acctttagca tttggaaaaa ttataaatga ctttttaatt
     1681 gtaaataccc tcaccccttt tgataattta aattttgtta ttaagtgggc ctttgtaatt
     1741 ctttattatt ttgggttgtt ttgcgtttat ttgaaatatg taagttttct taaaaatatg
     1801 ggttataaaa agtatagatt cttaattata ctttccccag ctattttttt aatggtttat
     1861 tattttatag aatggctgtt tagttaaatt atttttttct aaatcagacc attcaactat
     1921 atttttattt gctggcaaca acattccatt taataaatta aaaaatagta ttgctttttg
     1981 taggagacta aaaaagtacc ctataacact tttcaggctt gatgtagaag ttatctgcat
     2041 gttgaatatt cattagcccc tccctttact ctgttatcaa aagcttagat ttaatcgaat
     2101 ttatggcatt aatggctctt tctatttcta tcaaacctct aaatctatct ttagaaaagg
     2161 tttccgctgg attgcccttt gaaccttttt gcgtgtgttg gtcatgatcc attaaccatt
     2221 taaaatatgt aataaaacgg tcacaagttt tattgatatt ttcaggattt cctgaattga
     2281 tagcttccgc tagtgagtta aagttatgga ttgtatcagt gatccacatt aaatcttgac
     2341 tggattccat actcatcaca actcgatcaa atactttttg atcagtagta ggttgtggta
     2401 aatattgtgg tttattaaaa attctactta aaaaactctg tttttttaag tgatatacct
     2461 tatctatagg tcttgcttgt ggatgtgcaa attcactgcc gtaagcacta aatagccgtg
     2521 cagctgttcc tacaccagca gtaaaatgaa gtaattcact tagctcatgt gtgaaattat
     2581 attgcattgt tttaatcctc atattcattt atttgcaact caaaagaaac ggaacaatca
     2641 ccacaaatta catttaatga ttccttgccc caaaggttgt ttttacattt tggacacgta
     2701 aacttgaatt tactttttgg cggttttaac ggtctattta cgaattgtat gactggctca
     2761 gtcgcaccga gagggatact atcaagatca ccacttaaat caatttcagt tggtaaatga
     2821 gggatcacaa gcagactttc acttattccc tgggttaaat ctgttgctat ttgctgatct
     2881 tcaagcatgg tgtgaattga tgtgttctta ggcatgaatc tatcgtacca agcaacaata
     2941 atcccctcat tgaaaagatc attactcact tccaaaaact gaccgcctgg gatcgggtaa
     3001 tcagccatgt gctggcctgt atcgttccca cctggacgac cagtagaact tgccatcaaa
     3061 ccacgttctt tcatcatggc tgcaaattct ttattgtgat agcccttttg tgacggcttg
     3121 ccatagctca tttgatacag atgcaccagt tcatggcata tcgtttggaa taactcaatt
     3181 aagggtttta caccaaaaaa ttcaggattt agagctaatt catgagcaaa ttgattcttg
     3241 tcatcaatag aaataaatcg gttgtaagac atataaccga atgaatttgc ctttcgttga
     3301 agtgtgatta agctattctc tagctttcca tcaaataacc tatcattgaa agtatcataa
     3361 acatgctgta aagcttcata aacttgtggt gtaggtttat tcataaatca aacaccattt
     3421 caaccaataa attcagcatt cattgctttt atttttctta aatcttcatc actaacaaca
     3481 tgatcaccaa tttcagaaag tagcccccaa acttcccaat aaaatgaatg ctgaatacgt
     3541 gtttcattgt taaaaatgaa tgatattttt ttgcccataa gtttaagcgc tttacgatgt
     3601 tccattttca cctccccaat aaagctctaa ttaattaaca ttattaactt cccaaccatc
     3661 ccattttcca ccactttcca tgatgcttaa caaagatttt tcttggttat tccaatccca
     3721 tttaccccaa tatttttttg tttcactagg acagccttta tcaataaaaa ccccaaaacc
     3781 gaaaagcatt ccttgagatt gttttacaaa aactttacag cgatcatcat tttcgtcata
     3841 aagtaaatga ccattatcta taaccagtgc cattaaaatt ctcctattac ctgtcaatca
     3901 gtagagctaa aatttttaac tgttttagcc cattgttcaa acaatgcgcc accattcacc
     3961 aaccccttat attcaggctc atctaaatac tcatgcactt ctcttgttgg agagcgatcc
     4021 atagctagga tagccatgca gtcttgaaac agattcaagt caagttgacg aaaatccgtt
     4081 aagtcaaatg gaaatctttg accgtcatag cagccaagca gaaatttacg aactacaagg
     4141 ctttgccctg tatcagattt tgccacttca accaatcgtt tgaggatcgg tacacccttt
     4201 gcttgggcat cttgtttaaa cgattcttcc tggttcaatt gctggagtaa tttgtctttc
     4261 attgtgtcca ggtcttgctg tgttggttga aatagtttcg ggccagtcat atcaattctc
     4321 ctttttgaat tattaacatt acgatattga taaaactaaa caaccctctt tattgaacaa
     4381 tgtgacaggg catgtcgcat aatttaattt tagttatatc gtacgatata attatacttc
     4441 taaataaaaa atctatcaat agactatacc taaatgaaaa tcttatataa tgatagacaa
     4501 ggctatcatt aaagtattta taatagctaa acgattgttt tatcataaaa catttaaaat
     4561 taatgtttaa tgatagtgct tggcgacaca taacctgtca atgaccgaca catagttgtc
     4621 attggcaacc aaattggaaa attgcgaaat ggtaactaga atttacgtta gagccagtac
     4681 aaaagatcaa gatgcagaac gtgctttaca aatgctaaaa gattttacca ctggcgaatt
     4741 ttcaacctac atcgaaaatt atactggaac aaaattaaat cgaccc
//
LOCUS       DAOMTA010000135         4784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_454_length_4784_cov_2.584690, whole genome shotgun sequence.
ACCESSION   DAOMTA010000135 DAOMTA010000000
VERSION     DAOMTA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_454_length_4784_cov_2.584690"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLK68_05005"
     CDS             complement(<1..235)
                     /locus_tag="PLK68_05005"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRM89805.1"
                     /translation="MKMTFRWYGTDDPVSLEYIKQIPNITGIVSAVYDIPVGEVWPIE
                     KIEKLKAEVNLVGLELEVIESVPVHEDIKLKRND"
     gene            complement(253..1014)
                     /locus_tag="PLK68_05010"
     CDS             complement(253..1014)
                     /locus_tag="PLK68_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM89806.1"
                     /translation="MKLNRTLLRAIEILELLSRSKEGYTLTELSLIFDYPKSSVFDII
                     KTLVYKNMVVEDNQTGITKYKIGLASFLIGSSYLNNIDIVNIAKSNLIDFANKMNATT
                     FMAVLDENMVTYIYKYESENSIITTANVGTRKSLHCAALGKAMLAYKSEEEINKIIDK
                     IDFISYTYFTIKTKEKLIEELAEVRQRGYAKDDRENTLQQIAVAAPLFDHEGHVVAAI
                     SCVGFYESSIDLDDLGLLIKDVGKQISYKLGYNPQ"
     gene            complement(1181..1477)
                     /locus_tag="PLK68_05015"
     CDS             complement(1181..1477)
                     /locus_tag="PLK68_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89807.1"
                     /translation="MASKVKKKQNLQGLTEQQKHIIKLRNELNKPDPHQVKAFTLYKI
                     ITYVFNVLFPPYALYRIWCKKSEFTKIERYAQSVVAVTILCMFVLLQLERYKII"
     gene            complement(1467..2258)
                     /locus_tag="PLK68_05020"
     CDS             complement(1467..2258)
                     /locus_tag="PLK68_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM89808.1"
                     /translation="MKLNRTALRTIKILEYIANQKDGCTLLEITEALDIPKSSAFDIV
                     KTLLYKKMIVEDQNHGKLKYKMGINAFIIGSGNIERLDLVEAAKNQLIETANHFNATA
                     FLAILDNKMVTYLYKYEAPHRIVTNANIGTRKPIYSTALGKCLLAFQRKPEVIKDILK
                     EIDFKPLTEYTITSPQKYLKELKRVKNIGYAVDFQEDSIYQICIAAPIFNHNKNVVAA
                     ISCTFLYDTNLRIKEIGEEMKRIALTISKKLGYIDDTGRRNIHGK"
     gene            complement(2314..3633)
                     /locus_tag="PLK68_05025"
     CDS             complement(2314..3633)
                     /locus_tag="PLK68_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM89809.1"
                     /translation="MKISDSLTEKLLVVASKISNQKHMYAIKTAFTTLMPVIITGAFC
                     TLIVNVVCSTETTGISLAKVQGFSWLEMFTDLFNAANYATLNFFTIAAVVLIGLELGV
                     KNGIKGFMTGIVAVCSFVACLSTNIVATVGEESITVAGIAKDYTASKGLFLGMIIALL
                     SVELFTKLCKSKYLKINMPDSVPSNVTSSFNNLFPFALTIIIFAAINYTVVQLAGLSL
                     YDIIYTFIQKPLQAVVQGLPGVLLLMLVAQIFWCVGIHGNQIIKPVRDPLLNAAILAN
                     TDIVANPNYNPADLNIISMSFWDTYGNIGGSGCVVGLLIACLVFSKREDYKQVAKLSI
                     APNIFNISETLHFGLPIMLNPLLMIPFIITPLATMAFGYFMTIIGFSDILVYAFPWTT
                     PPFINAWIASGGSIGTVITQALCIVISIAIYTPFVIIANKQKDIVSQ"
     gene            complement(3769..>4784)
                     /locus_tag="PLK68_05030"
     CDS             complement(3769..>4784)
                     /locus_tag="PLK68_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HRM89810.1"
                     /translation="LSGKYDLEKTAVIISQTGGGCRATNYIAFIRKALQDAGMPQVPV
                     ISANLQGLENNPGFKLTLPLIKKVVIGAMYGDIFMRVLYRVRPYEVIPGSANDLYQSW
                     VERCQENVKNGSIKQFRKNVYQIVKEFDELPLLDIKKPRVGLVGEILVKFHPTANNEI
                     VEIIEREGGEAVMPDLIDFFQYCFYNTDFENEHFHASKNSARICNLAIKFVDLLRHDM
                     IKALKKSNRFDPPASIQHLAKKASSIVSIGNQTGEGWFLTGEMIELIESGAPNIVCMQ
                     PFGCLPNHVTGKGAIKALRKAYPESNIVAIDYDPGASEVNQLNRIKLMLSTAFKNMNK
                     KPE"
BASE COUNT         1581 a          884 c          609 g         1710 t
ORIGIN      
        1 aatcatttct ctttaattta atatcctcat gcaccggaac actttcaata acttccagct
       61 ctaagccaac taaatttact tcggctttta gcttttcaat cttttcaatc ggccatactt
      121 ctccgaccgg tatatcatat actgcactca caattcctgt gatattagga atttgtttaa
      181 tatattctaa acttacagga tcatccgttc cataccagcg aaatgtcatt ttcataatta
      241 ccaactcctt ccttactgag gattatagcc taatttatag gaaatctgtt tacccacatc
      301 tttaataagt aatcctaaat catctaaatc gatactactt tcataaaaac caacacaact
      361 gatggcagca acaacatgtc cttcatgatc aaaaagtggt gcagccacag ctatttgttg
      421 cagagtgttt tctcgatcat cttttgcata gccccgttgc cttacctctg ctaattcttc
      481 aataagtttc tctttagttt tgattgtaaa atacgtatat gaaataaaat caatcttatc
      541 aatgatttta ttaatttcct cttcgctttt ataagctaac atagcttttc ctaatgctgc
      601 acaatgaagt gattttcttg taccaacatt agctgttgta ataatactgt tttctgactc
      661 atatttataa atataagtca ccatattctc atctaaaaca gccataaaag ttgttgcatt
      721 catcttgttt gcgaaatcaa ttaaattact tttagctata tttacaatat ctatattatt
      781 taaataacta cttccaataa gaaacgatgc taacccaatc ttatattttg taataccggt
      841 ttgattatct tcaacaacca tatttttata tactaatgtt ttgatgatat caaaaacact
      901 tgactttgga taatcaaata ttaatgatag ctccgttaaa gtataccctt ctttacttct
      961 tgaaagtaat tctaatattt ctattgctct taataatgta cgatttagct tcattttttc
     1021 ctccatctcc attgccatca atttagtata taccaattaa tttttagcaa gtttataatt
     1081 acgaatttga ttcgtaatat cgtctattta tactataatt tcttttgtag tggaggtcaa
     1141 cattctttac ttgattttat aaattgataa cgatttccca ttaaataatt ttatatcttt
     1201 ctaactgcaa taaaacaaac atacataaaa ttgtcactgc aacaactgat tgggcatagc
     1261 gttctatttt tgtgaattcc gatttcttac accagattct atataatgca tatggcggaa
     1321 ataaaacatt aaaaacataa gttataattt tatatagtgt aaaagcctta acttgatggg
     1381 gatcaggttt gtttaattca ttccgcaatt ttataatatg tttttgttgt tctgttaaac
     1441 cttgcaaatt ttgtttcttt tttactttac ttgccatgaa tattcctcct gcctgtatca
     1501 tctatataac ctaacttttt agaaatagtc agtgctatcc ttttcatttc ttcaccaatc
     1561 tctttaattc ttaaattagt atcatataaa aaagtgcagc ttattgcagc aacgacattt
     1621 ttattgtgat taaaaatagg tgctgctata caaatttgat aaatagaatc ttcctgaaaa
     1681 tcaaccgcat atcctatatt tttaaccctt tttaattctt taagatactt ttgagggcta
     1741 gtaatcgtat attctgttaa cggtttaaaa tcaatttctt ttaaaatatc tttaataact
     1801 tctggttttc tttgaaaagc taacaaacat tttcctagtg ccgtactata gattggtttt
     1861 ctcgtaccaa tattagcatt agtgactata cgatgtggcg cttcatactt atataaatat
     1921 gtaaccattt tgttatctaa gatagcaaga aatgccgttg cattaaaatg attagcagtt
     1981 tctatcaatt gatttttggc tgcttctaca agatctaagc gctcaatatt cccgcttcca
     2041 ataataaaag catttattcc cattttatat tttaatttcc catgattttg atcctcgaca
     2101 atcatttttt tatacagtaa tgtcttaaca atatcaaaag cacttgattt aggtatatct
     2161 aaagcttctg ttatttctaa caaagtacat ccatcttttt gattagctat atattcaaga
     2221 attttaattg ttcttaaggc tgtacgatta agtttcattt aattttcctt ctctaatgta
     2281 tgtacgaaaa gccgctaatg cggcttcagc cttctattgt gaaacaatat ccttttgttt
     2341 attagcgata ataacaaatg gagtataaat agcaatggaa ataacaatac atagtgcttg
     2401 ggtaattact gttccaatac tgccacctga agctatccag gcattaataa atggcggggt
     2461 tgtccaaggg aaagcatata ctaatatatc tgagaatcca ataatcgtca tgaaatatcc
     2521 aaatgccatt gttgctaatg gggtaataat aaaaggaatc atcaataatg gattaagcat
     2581 aattggtaaa ccaaaatgca aagtttcaga aatattgaaa atatttggag caatagacaa
     2641 cttagctact tgtttataat cttcacgttt tgagaagact aaacatgcaa tcaacaaacc
     2701 aacaacacaa cctgaaccac caatattacc ataagtatcc caaaacgaca tagaaataat
     2761 attaagatca gccggattat agttcggatt tgcaacaata tccgtattag ctaaaattgc
     2821 tgcattcaat aacgggtccc ttactggttt aataatttga ttaccatgaa tacctacaca
     2881 ccagaaaatt tgtgctacta acattaacaa taagactcct ggtaaccctt gtactactgc
     2941 ctgcaatggt ttttgaataa aagtatagat gatatcatat aaacttagtc ccgctaattg
     3001 aaccactgta tagttaatcg ctgcaaagat aataattgtc aatgcaaatg gaaataagtt
     3061 attaaaactg cttgttacat ttgaaggtac tgaatctggc atattaatct tcaaatattt
     3121 acttttacat aattttgtaa atagttctac cgaaagcagt gcaataatca ttcccaaaaa
     3181 caatccttta gaagccgtat aatctttggc aattccagca accgtaattg actcttctcc
     3241 tactgtagct acaatatttg tacttaagca tgcaacaaaa ctacaaaccg ctactattcc
     3301 cgtcataaac ccttttattc catttttaac tcccagctct aatccaatta aaacgacagc
     3361 tgcaattgta aagaaattta aagtagcata atttgctgca ttaaatagat cagtgaacat
     3421 ttccagccaa gaaaaccctt gcacctttgc taaactaatc cctgttgttt ccgtcgaaca
     3481 aactacatta acaatcaacg tacaaaaagc tccagtaata attaccggca tcagtgttgt
     3541 aaaagctgtt ttaattgcat acatatgttt ttgattacta atcttgcttg ctacaactaa
     3601 taatttttca gttaacgaat ctgaaatttt cactttcctt ctcctttcat atatacgaac
     3661 ttagttctta tatatgtatt ttagaaaata aagttatcaa tgtcaacgaa tcaagtgtat
     3721 ttgtgtacaa tatccgtata tacgtaaaat agtacaaaaa aaaccagatt actctggttt
     3781 tttattcatg ttcttaaatg cagttgaaag cattaattta atacgattta attgattaac
     3841 ctcacttgca ccaggatcat agtctattgc cacgatatta ctttcaggat aagctttgcg
     3901 taaagcttta attgcccctt tacccgtaac atggtttggc aaacatccga atggctgcat
     3961 acaaacaata ttaggagctc ctgattcaat tagttcaatc atctcacccg ttaagaacca
     4021 tccttctccc gtctgatttc caatcgaaac aatcgaacta gcttttttag ctaaatgttg
     4081 gattgatgca ggaggatcaa aacgattaga tttctttaaa gctttgatca tatcatgacg
     4141 aagtaaatca acaaacttaa ttgctaaatt acaaattcgt gcagaatttt tagaagcatg
     4201 aaaatgttca ttttcaaaat cagtattata gaaacaatat tggaagaaat ctattaaatc
     4261 aggcattact gcttcgccac cttcacgttc aattatttct acaatttcat tatttgcagt
     4321 tggatgaaat tttactaaga tttcaccaac taaaccaacc cgtggttttt ttatatctaa
     4381 tagtggtaat tcatcaaatt ctttaacaat ttgataaaca ttttttctaa attgtttaat
     4441 acttccattt tttacatttt cttgacatcg ttcaacccat gattgatata aatcatttgc
     4501 acttcctgga attacttcat aaggtcgaac tcgatataat actcgcatga aaatatcacc
     4561 gtacattgcc ccaattacta cctttttaat tagtggcaaa gttaatttaa atccaggatt
     4621 attttccaaa ccttgaagat tggcagaaat aacaggtact tgaggcattc ctgcatcttg
     4681 caatgcttta cggataaagg cgatataatt agtagcacgg catcctccgc cagtttgcga
     4741 tataattaca gcagtctttt ctaaatcata cttaccactc aata
//
LOCUS       DAOMTA010000136         4766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_462_length_4766_cov_2.490129, whole genome shotgun sequence.
ACCESSION   DAOMTA010000136 DAOMTA010000000
VERSION     DAOMTA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_4766_cov_2.490129"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1138)
                     /locus_tag="PLK68_05035"
     CDS             complement(<1..>1138)
                     /locus_tag="PLK68_05035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM89811.1"
                     /translation="RIYPNGNGEVNEAGLQFYDNLINECLKYGIEPMVTVYHWDMPQA
                     LEEQYHGWENRQIVDDYVNYATTLFKRYGDRVKYWITMNEQNIFTSFGWLEGMHPPGK
                     VDDMKMFYQVNHHANMAHAKSVIALKELWPEAKVGASFAYSPSYAIDNKPENAMAKAD
                     YDDLKNYYWMDVYAYGRYPRAALAYLEANGVAPVFEEGDNAIMKEAAQLVDFMGVNYY
                     QTTTVEFNDIDGVGTSHEMNTTGKKGTAKVQGVPGLYKNPQNANLPTTDWDWTIDPMG
                     IRMCCREITSRYDLPIVISENGLGAFDKLEDGEVHDPYRIAYLKAHIEELKKACDDGC
                     RVLAYCTWSYTDLLSWLNGYQKRYGFVYVDREEDENSGTLNRYK"
     assembly_gap    1139..1151
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1152..1401)
                     /locus_tag="PLK68_05040"
     CDS             complement(<1152..1401)
                     /locus_tag="PLK68_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89812.1"
                     /translation="MYFKDSKGFPSDFLWGSASAAYQVEGAWDSDGKGVSNWDKFVRI
                     PGKTFKGTTGDKAVDHYNRYKEDVALMAEMGLKTYRFSI"
     gene            complement(1391..3610)
                     /locus_tag="PLK68_05045"
     CDS             complement(1391..3610)
                     /locus_tag="PLK68_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM89813.1"
                     /translation="MDNKLKIGLLVDDSDNIFTTEIWRGASYAAGKLDVNLVVFFGGF
                     VGSSNIYGNSRYEFQKNTTYKFSKTKDLDLLIISVSSIVHGNNRLKELFVKEFEGVPI
                     VTLNHKFGNNSLVTFDNAKGIEDAVTSLIQEQQCRKIGMIAGPYENKGSDERLAAYKK
                     TLKSHGIVIDEKRIIHTATFERGYPEYAKKLLDLNPDLDAIVCASDNLAFDAYQVLKE
                     RGIRIGEDVQVVGFDDVQEASKVNPPLATIRAEAAQLGFYAVMDGVLSLRGEIPAEFT
                     TLVDVDFIKRDSAAKVGYLEERLYRQIVNYTKSDKDKIVNILMEYIFNNNHSIFIIEA
                     RKRVIDLVTFLVELHNDDLFEEKTYTKFSNLIQELIDLDSVEFIDLKRILKVIDVVSQ
                     RLSDYRNNLSIIHFNQKILQIIGMHYNTILSERTRISCEEKRLVNILSRGMLSVRNEY
                     DNYNTIFECLKQLNIGNARLYLYEKPITSYMTQFTVLPNEVILKGSIINGKIIIPDDK
                     ILVKTDRIFSEKRLFSNCNEYVVNSVYSGTTQYGIFVCDLKYRDFVDLDFVSSQLGTV
                     VNTINLVEKLDNLSKHDELTGLWNRRGFIDKVQGYMNINKGALIFVDLDGLKIINDTY
                     GHEGGDEAIITGAKILKDAFDEFGVIGRIGGDEFAVFLPNQSDFALSEIDQLINDKTI
                     YHNTLIKRNFKVELSYGISLFDQYQDLTVRELLDKADREMYCHKRNKKGNRRKKDVF"
     gene            complement(3624..4529)
                     /locus_tag="PLK68_05050"
     CDS             complement(3624..4529)
                     /locus_tag="PLK68_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM89814.1"
                     /translation="MDKKGKKVVTSLAVLAGLASGAGALNAFANKTVKAMLYRHHKED
                     DKPSILETKYDAQSIYIKNHQGIRLRGVLIEAVDAKKTLVILHPFALEAKDMTLYVPF
                     FKERYPDWNILLVDACAHGQSDGYIRGLGIKDVKDLVCWNEYLLKTYGKEHEIILYGK
                     EAGANTILKAASKHLLKNVKAIISDGAYTSVYDILGYRMIKDYKVPKFPTMRLIKRKI
                     KQEIKVNIKEDYAEMVKHNDIPTLYIHMKEDDFVPLSMVYPLYNANRGSKVLFVLKDE
                     RYLYELEETDEFRKTLANFITKYVN"
     gene            complement(4522..>4766)
                     /locus_tag="PLK68_05055"
     CDS             complement(4522..>4766)
                     /locus_tag="PLK68_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89815.1"
                     /translation="GYDLLINLFSKDYNTFVFVCFPISTFPPEYREPIREELEKAIRF
                     YAGRMEHDEYRLSSFEQVSNINRLLMENYKEYGKNG"
BASE COUNT         1550 a          955 c          573 g         1675 t
ORIGIN      
        1 ttcttataac gatttaaagt accgctattt tcatcttctt cacgatcaac gtaaacaaaa
       61 ccataacgct tttgatatcc atttaaccag cttaataaat ctgtatatga ccatgtacaa
      121 taagcaagaa cacggcatcc atcatcacaa gccttcttca attcctcaat atgtgctttt
      181 aaataagcaa ttcgatatgg gtcatgtact tccccatctt ctaacttatc aaaagctcct
      241 aaaccatttt cactaataac aattggtaaa tcatagcgtg atgtaatttc acgacaacac
      301 attctaattc ccattggatc aattgtccaa tcccaatcag ttgttggaag attagcattt
      361 tgaggatttt tataaagtcc tggtacacct tgaactttag cagtaccttt tttaccagtc
      421 gtattcattt catgacttgt tccaacacca tcgatatcat taaattcaac tgtagttgtt
      481 tgatagtagt taactcccat gaaatcaact aattgtgcag cttctttcat gatagcattg
      541 tcaccttctt caaaaacagg ggcaacacca ttagcctcta aataagcaag agcagcgcga
      601 ggataacgcc cataagcata aacatccatc caataataat tcttcaaatc atcataatct
      661 gctttagcca ttgcattttc tggtttattg tcaattgcat aagatggact ataagcaaaa
      721 cttgctccaa ctttcgcttc tggccataat tcttttaatg caattacact tttagcatgt
      781 gccatatttg catgatgatt aacttgatag aacattttca tatcatctac ttttcctggg
      841 ggatgcattc cttctaacca gccaaagcta gtaaagatat tctgttcatt catggtgatc
      901 cagtatttaa cacgatcacc atatcgttta aataaagtag ttgcatagtt tacataatca
      961 tcaacaattt gacgattttc ccaaccatga tattgctcct ctaaagcttg aggcatatcc
     1021 cagtgatata ccgtaaccat tggctcaatt ccatatttta aacattcatt aattaaatta
     1081 tcatagaatt gtaaaccagc ttcattaacc tcaccgtttc catttggata aattcttgnn
     1141 nnnnnnnnnn ncaattgaaa aacgataagt ttttagaccc atttctgcca ttaaagcaac
     1201 atcttcttta taacgattat aatggtctac agctttatca cctgttgttc ctttgaaagt
     1261 tttcccaggg atacgtacaa atttatccca gttggaaact cccttaccat cactatccca
     1321 agctccttct acttgatagg ctgccgacgc actgccccaa agaaagtcac ttggaaagcc
     1381 tttactgtct ttaaaataca tcttttttcc tcctatttcc ttttttatta cgcttatggc
     1441 aatacatctc ccgatctgct ttatctaaga gttcacgaac tgttaaatct tgatattgat
     1501 caaaaagact aattccataa gataattcta ctttaaaatt acgttttatt aaagtgttat
     1561 gatagattgt tttatcattt attaattgat caatttctga aagagcaaaa tcactttgat
     1621 ttggtaaaaa tactgcaaat tcatcaccgc caatacgccc aattacacca aattcatcaa
     1681 aagcatcttt aagaatctta gctcccgtga taattgcttc atcaccacct tcatgaccat
     1741 aagtatcatt gataattttt aaaccatcaa gatccacaaa aatcaatgca cctttattaa
     1801 tattcatata tccttgtact ttatcaataa aacctcggcg attccatagc cccgtcagct
     1861 catcatgttt tgataaatta tctaacttct caactaaatt gatcgtattt acaactgttc
     1921 ctaattgact agaaacaaaa tctaaatcta caaaatcacg atatttcaaa tcacaaacaa
     1981 atattccata ctgtgttgtt ccagaataaa ctgagttaac aacatattca ttacaattac
     2041 taaacaatct cttttctgaa aatattcgat cggttttaac cagaatttta tcatcaggaa
     2101 taataatctt accattaatt atacttcctt tcaaaataac ctcattgggt aaaactgtaa
     2161 attgtgtcat ataactagta attggttttt catacaaata taatcttgca ttaccaatat
     2221 tcagctgctt taaacattca aaaatagtat tgtagttatc atactcgttt cgaacactga
     2281 gcattcctct tgataaaata ttaaccagcc gtttctcttc acaagaaatc cttgttcttt
     2341 cacttaaaat agtattataa tgcatgccaa taatttgtag gattttttga ttgaaatgaa
     2401 taattgatag attattacga taatcactaa gacgctgact aaccacatca ataactttta
     2461 aaattctttt taaatcaata aactcaacac tatctaaatc tattaattct tgaattaaat
     2521 ttgaaaattt tgtatatgtt ttttcttcga ataagtcatc attatgaagt tcaactagaa
     2581 aagtaactaa atcaattacg cgtttgcggg cttcaataat aaaaatactg tggttattat
     2641 taaaaatata ctccattaaa atattaacga ttttatcttt gtcgctcttt gtataattaa
     2701 ctatttgacg atataatcgt tcttctaaat aacccacttt agcagccgaa tctcgtttaa
     2761 taaaatctac atcgacaagt gttgtgaatt cagctggaat ttctcctcgc aatgataaaa
     2821 ccccatccat gacagcataa aatcctaact gtgctgcttc ggcacggata gttgctagag
     2881 gcgggtttac ttttgatgct tcttgaacat catcaaaacc aactacttga acatcttcac
     2941 caattcgaat ccctcgttct tttaaaactt gatacgcatc aaaggcaaga ttatcacttg
     3001 cacaaacaat agcatccaaa tcaggattca aatccaataa tttcttggca tattcaggat
     3061 aaccacgttc aaatgttgct gtatgaataa ttcttttttc gtcaatgacg attccgtgac
     3121 tttttaatgt ttttttatat gcagctaatc gctcatccga ccctttattt tcataaggac
     3181 cagcaatcat cccaatttta cgacattgct gttcttgaat taaactagtt actgcatcct
     3241 ctatcccttt agcattatca aatgttacta gtgaattatt tccaaattta tggtttaaag
     3301 taacaattgg cacaccttca aattctttta cgaataattc ttttaaacga ttattcccat
     3361 gaacgatact tgagacactt ataattaata aatctaaatc ttttgtcttg gaaaatttat
     3421 aagttgtatt tttttgaaac tcataccttg aattcccgta gatgtttgag cttcctacaa
     3481 atccaccaaa aaatacaact aaattaacat ccaattttcc ggctgcataa ctcgctcccc
     3541 gccaaatttc agtagtaaag atattatcgc tatcatctac cagcaagcca atttttaatt
     3601 tattgtccac aaataccacc agcctaattt acatacttag taatgaaatt cgctaatgtc
     3661 tttctaaatt catcggtttc ttctaattca tataaataac gttcatcttt taaaacaaat
     3721 aatactttgc taccacggtt cgcattatat aatggataga ccatacttaa tggtacaaaa
     3781 tcatcttctt tcatgtggat atataatgtt ggaatatcat tatgtttgac catttcagca
     3841 taatcttctt taatattaac tttaatttcc tgtttaatct tacgcttaat taaacgcatt
     3901 gttggaaatt ttggtacttt ataatcctta atcattcgat agcctaaaat atcatacaca
     3961 cttgtatagg ctccatcact aataatagct ttaacatttt ttaataaatg tttactagct
     4021 gcttttaaaa tagtattagc accagcttct ttaccatata aaattatttc atgctctttt
     4081 ccgtatgttt ttaacaaata ttcgttccag caaacaagat cttttacatc tttaatacct
     4141 agtccacgga tatatccatc actttgacca tgggcacaag catcaactaa taagatattc
     4201 caatcagggt atcgttcttt aaaaaacggt acatataaag tcatatcttt agcctccaga
     4261 gcaaatggat gcaagattac taaagttttt ttcgcatcaa ctgcttcaat aaggacacct
     4321 cttaatctaa taccttggtg attttttata taaatgcttt gcgcatcata ttttgtttct
     4381 aaaatacttg gtttatcgtc ttctttatga tggcggtata acattgcttt aacggtttta
     4441 ttagcaaagg catttaaagc tccagccccg gatgctaacc cagctaaaac tgctaatgag
     4501 gtaactactt tttttccttt tttatccatt tttaccatac tccttataat tttccattaa
     4561 taatcgattg atgttactaa cttgttcaaa agagcttaga cggtactcgt catgttccat
     4621 tcttcctgca tagaaacgaa tggctttttc taactcttca cgaattggtt cacgatactc
     4681 tggtgggaaa gtagaaatcg ggaagcaaac aaaaacaaat gtattataat cttttgaaaa
     4741 taaattaatc aataaatcat atcctt
//
LOCUS       DAOMTA010000137         4743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_464_length_4743_cov_2.189846, whole genome shotgun sequence.
ACCESSION   DAOMTA010000137 DAOMTA010000000
VERSION     DAOMTA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_4743_cov_2.189846"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..997
                     /locus_tag="PLK68_05060"
     CDS             62..997
                     /locus_tag="PLK68_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89816.1"
                     /translation="MDKLIVIEHMDKVFGNYKAIDDLSFSLEGGKIYGMIGPNGAGKS
                     TTMSLLMGLIFPTKGSGLIKGYPLGSNEAKAIMGYSPEFPNFYSDMSCLEYLVYMGML
                     SDLSYDEALKRTKELLEEFDLVEHQLKRVAKFSTGMKKKVGLIQAMMHKPEILLLDEP
                     TANLDPTSRYEIIQTLKRLVKQRKMTVLISSHVLTELEMIIDHVIMINKGHVVLNQPI
                     DVVQNEFNQEKLLVSCNHNEHLQTYLETKGYFYTLNNGVLRVSIDDKQKCKKEIVKFI
                     YENDYELDLLKEDTISLEGLYQQLVEENDHESTIQ"
     gene            978..1889
                     /locus_tag="PLK68_05065"
     CDS             978..1889
                     /locus_tag="PLK68_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM89817.1"
                     /translation="MKVLFSKTFIKMFKLIPVVACVIISGFFGFILSNQYLGLMSQKP
                     TYLSLSQDILMFYAVLAFVLLVGMLIWIICSNSGSGLFASEIHEGTMRLLLSKKISRL
                     ELVIGKVGGMLVGSFVYLVISFATFLMVFIILSGVEKDILRMVLAATLAFISYGIILI
                     FILGGLATFLSSCFKKKVPAILILVALAALIFGIIPFARIFLTNTGKYDTYHLYLFDI
                     NYHLSLIFNQFLMLVGDFNTMLGDLSMFGMFTNLYSYGVTDFDLGNRAVYTLNQSLNS
                     GLITAVYLALASGLYVLSYRRMDKKDI"
     gene            2130..3014
                     /locus_tag="PLK68_05070"
     CDS             2130..3014
                     /locus_tag="PLK68_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89818.1"
                     /translation="MYLVFDIGGTAIKYCWMNEAGEIFNKQEILSTAVDSLDKFIETV
                     AKIYHESNRKVEGIALSCPGVIDAANGTIKVVVAYPYLQGICLTELISKACDNIKVSL
                     ENDAKCAGLAEAWIGSAQAYDDAIIVVLGTGIGGAIIKNKQIHHGAHLFAGEISTLIV
                     DYDKETNQVLTWSDIASTTALCKRAAEALAVTSIDGRRVFELANNADEVVLEVLKNFC
                     LDIAIQLYNLQYSYDPGVICIGGGISKQPLLIKLIKEAVEIIANETNQLLKPNVTTCK
                     FYNEANLIGALSYFLSIK"
     gene            3161..3919
                     /gene="aroD"
                     /locus_tag="PLK68_05075"
     CDS             3161..3919
                     /gene="aroD"
                     /locus_tag="PLK68_05075"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRM89819.1"
                     /translation="MGVCKVKNIVIGEGMPKICVPVVSNNHQDIITDLIRLQSLDIDL
                     IELRIDYFNELLDHQKLTELFKAIASMAIKQGIILTYRSVPEGGNGKLSNDEYMQLYS
                     IALESGAFDIYDVELSSGTNTIINLSNLIHQNDKKIIMSSHDFTRTPSIDTMLGKIKQ
                     MDSLEADIIKIAVMPEDYRDVLLLLETTLKANELYDKPLVTMSMSSKGIATRILGEQF
                     GSAITFGKDNNSSAPGQIEVHALKEVLKVIHHNN"
     gene            3984..4361
                     /locus_tag="PLK68_05080"
     CDS             3984..4361
                     /locus_tag="PLK68_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89820.1"
                     /translation="MLINQNELEKLCKYLYPKFFDYKDISLYDLNIKIDDYLHLSANL
                     NYYNIETKLRAIARVRVEDKIIIDINGVIKYGFINLDLNKVLKETVKDLPYLTINDES
                     IIIDNEYVREISLKDEYINIELK"
     gene            complement(4370..>4743)
                     /locus_tag="PLK68_05085"
     CDS             complement(4370..>4743)
                     /locus_tag="PLK68_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRM89821.1"
                     /translation="SYLYNVPWEYMYHNDIEKGLLRDAFKDFLPSDIYNRKKNPYPKT
                     HSPLYRDLIVELLKESLNDENNLLLRLFNRDKLIDLINSGGESFKYPWFGQLMTGPQL
                     LAYFYQIYLWGRIYHVEIELE"
BASE COUNT         1658 a          549 c          868 g         1668 t
ORIGIN      
        1 gagaaggtat atttgtagat gactattaaa tgagtcaatt acaatatacc aggtgttaaa
       61 catggataaa ttaattgtga ttgaacatat ggataaagtt tttggtaatt ataaagcaat
      121 tgatgatttg tcttttagtt tagagggagg aaaaatttac ggtatgattg ggccgaatgg
      181 agcaggaaag tcaacaacta tgtcattgct gatgggcttg atttttccga ccaaagggag
      241 tggtttgatc aaagggtatc cgttaggttc taatgaagca aaggcaataa tgggatattc
      301 tccagaattt cctaattttt atagcgatat gagctgttta gaatatttag tctatatggg
      361 gatgttgtct gatcttagct atgatgaagc tttaaaacga actaaggaat tattagaaga
      421 atttgattta gttgaacatc agttgaaacg tgttgctaaa ttttcaacag ggatgaaaaa
      481 gaaagttgga ttgattcaag caatgatgca taagccggag attttattac tggatgaacc
      541 aaccgctaat ttagatccaa ctagtcgtta tgaaattatt caaactttaa aacgtttagt
      601 aaagcagcgg aagatgacgg ttttaattag ttcacatgtt ttaacggaat tagaaatgat
      661 cattgatcat gttattatga tcaataaagg tcatgtagtt ttaaatcaac ctattgatgt
      721 agttcaaaat gaatttaatc aagaaaaatt acttgtaagc tgtaatcata atgagcattt
      781 acaaacatat ttagaaacta aagggtattt ttatacgctc aataatggag ttttaagagt
      841 aagtatagat gataaacaaa aatgtaaaaa agaaattgtt aagtttattt atgaaaatga
      901 ttatgaatta gatttattaa aagaggatac gatttctcta gaaggactat atcagcagtt
      961 agtggaggaa aatgatcatg aaagtactat tcagtaagac atttattaaa atgtttaagc
     1021 ttattccggt tgttgcgtgt gtgattatta gtggcttttt tggttttata ctatcaaatc
     1081 aatatcttgg tttaatgagt caaaagccaa cttatctttc tttatcacaa gatattttaa
     1141 tgttttatgc agttttagct ttcgttttat tagtaggaat gttgatctgg attatttgtt
     1201 cgaatagtgg ttcgggactt ttcgcaagtg aaattcacga ggggacaatg agattattat
     1261 tatcaaaaaa aatatcacgt ttagagttag taatcggtaa agtaggtggt atgctagtgg
     1321 gcagttttgt ttatttggtt atttcttttg cgacattctt aatggtcttt attattttaa
     1381 gtggtgttga aaaagatata ttacggatgg tattagcagc tactcttgcc ttcattagtt
     1441 atggaataat tttaattttt attcttgggg gattagcgac ttttctatca tcatgtttta
     1501 aaaagaaagt acctgctatt ttaattctag tagcattagc tgcactaatt tttggaatta
     1561 tcccttttgc ccgaattttc cttacgaata ctggaaaata tgatacctat catttatatt
     1621 tatttgatat taattatcat ctttcattga tctttaatca atttttaatg ctggttggag
     1681 attttaatac aatgttaggt gatctaagca tgtttggaat gttcacaaat ctttactcat
     1741 atggtgtaac tgattttgat ttaggaaatc gtgctgttta tacattgaat cagtcactaa
     1801 atagtggttt gattacagca gtttatctag ccttagctag tggactgtat gttttatctt
     1861 atcgccggat ggataaaaaa gacatttaat ttagtactat ataataatac aatgaatctt
     1921 attatttgag taatgttttt gactaccgct tttttagtag caaagctgta tatccctttg
     1981 aacaatagag gttcctttac ggattataaa actagaagtt atttacataa taattttcta
     2041 gtttttttat gagaactttt ttcaaaataa gattaataaa cattccgatt aaaatctatg
     2101 atattattaa ttagtaggag gattgagcga tgtatctagt atttgatatt ggtggaacag
     2161 caataaaata ttgctggatg aacgaagcgg gagaaatttt taataaacaa gaaattttat
     2221 caacagcagt agacagctta gataaattta tagaaactgt tgctaaaata taccacgaaa
     2281 gtaataggaa agttgaagga attgctttat catgtcccgg tgttattgat gcagctaatg
     2341 gaacgatcaa agtcgttgtt gcgtatccat atttacaggg aatctgttta actgagctaa
     2401 tttctaaagc ttgtgataat ataaaggttt ctttggaaaa tgatgctaag tgcgctggat
     2461 tggctgaagc ctggattggg agtgcacaag cttatgatga tgcaataatt gtagttttag
     2521 gaactggaat tggtggagcg attataaaaa ataaacaaat tcatcatggt gcacatttat
     2581 ttgctggtga aataagtacc ctgatcgttg attatgataa agagactaat caagtattga
     2641 cttggtcgga cattgcttct acaacagctt tatgtaagcg ggcagcggaa gctttagcag
     2701 tcacgtcaat tgatggaaga cgtgtttttg aattggctaa caatgcggat gaagttgttc
     2761 tggaggtact gaaaaatttt tgtctggata ttgcaataca gttgtataac cttcagtata
     2821 gttatgatcc aggagtaatc tgtattggtg gaggaatttc taaacaacct ctattgatca
     2881 agctgattaa agaggcggtt gaaataattg ctaacgagac taatcagctg cttaaaccaa
     2941 atgtaacgac atgtaaattt tataatgaag caaacttaat tggagcgcta tcatactttc
     3001 ttagcataaa ataatttgtt tttttaccct aaaaaagtat catattatca tcaatagtgt
     3061 tatagtataa aaggtgaata ttggggaatg ttcattcagt attcttatta aaagaaacat
     3121 attgatgtta aaatgaatat ggtttttagg aggatgattt atgggtgttt gtaaagttaa
     3181 aaatattgta attggtgagg gtatgccaaa gatatgtgta ccagttgtta gcaacaatca
     3241 tcaagatatc ataacagact tgattagatt acagtcatta gatatagatt tgattgaact
     3301 tagaattgat tatttcaatg aactgttaga tcatcaaaaa ttaaccgaat tatttaaagc
     3361 aattgcttca atggcaatta aacaaggaat tattttaact taccgaagtg ttcctgaagg
     3421 aggaaatggt aaattaagta atgatgaata catgcaatta tattcaattg ctcttgaaag
     3481 tggagcattt gatatttatg atgttgaatt atctagtgga actaatacca ttattaattt
     3541 aagtaattta attcatcaaa atgataaaaa aatcattatg tctagtcatg attttactcg
     3601 tactcctagt attgatacaa tgttaggaaa gataaaacaa atggattctt tagaagctga
     3661 tataattaag attgcagtga tgccagaaga ctatcgtgat gttttattgt tattagaaac
     3721 aacactaaaa gctaatgagt tatatgataa accacttgtt acaatgtcta tgtcaagcaa
     3781 aggaattgca acaagaattt taggtgaaca atttggctca gcgatcacat ttggaaaaga
     3841 taacaatagc tctgctccag gtcaaattga agttcatgct ttaaaagaag tgttaaaagt
     3901 gattcaccat aataattaag gactatatgt cctttttttt gcaccttttt ataatttaca
     3961 cataaaataa aataggtggt taaatgttaa taaatcagaa tgaattagaa aaattatgta
     4021 aatatttata tccaaaattt tttgattata aagatattag cctttatgac ttaaatatta
     4081 agatcgacga ctatttacat ctaagtgcaa atttaaatta ttataatata gaaacaaagc
     4141 tgagagcaat agctcgggta agagtcgaag ataagattat tattgatata aacggagtta
     4201 ttaaatatgg atttattaat ttagatttaa ataaagtttt aaaggaaacg gtcaaggatt
     4261 tgccatattt aacgatcaat gatgaaagta ttattattga taatgagtac gttcgggaga
     4321 tatctttaaa agatgaatat ataaatatag aactaaaata atcgtaagat tattctagtt
     4381 ctatttcaac atgataaatc cgaccccaga gataaatctg atagaaatat gctaataatt
     4441 gaggaccagt catcagttga ccaaaccatg gatatttaaa ggattctccg ccactattaa
     4501 ttaaatcaat taatttatca cggttaaata aacgtagaag tagattattt tcatcattta
     4561 atgattcttt taacaattct acgattaaat cacgatataa tggtgaatgg gttttaggat
     4621 aaggattctt ttttcgatta tagatatcac ttggtagaaa atctttaaaa gcatctcgta
     4681 atagaccttt ttcaatatca ttgtgataca tatattccca tggcacatta tataaatatg
     4741 aaa
//
LOCUS       DAOMTA010000138         4741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_466_length_4741_cov_2.262484, whole genome shotgun sequence.
ACCESSION   DAOMTA010000138 DAOMTA010000000
VERSION     DAOMTA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_466_length_4741_cov_2.262484"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PLK68_05090"
     CDS             complement(<1..1603)
                     /locus_tag="PLK68_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRM89822.1"
                     /translation="MRSSIMKKSIIVAIILLILFGTLATLYINNLSDNLYSETDEYLN
                     DLTNQTAKAIKNRINENLTQLTTISLIIQQDGILQEPLLDYLNQLAQRDGVKRFGIAD
                     LQGNVVTSDHQTFNISDRDYFKDSLNGKTVTSKSITDYTDGEAINVYSVPIYKHNEVS
                     GVLFATFYTDKLSSILSSATYNNVGYAFIFNENGDVVLSNKKTDDFNNIESLNGIDLN
                     DIDINGKGIINFRDENNTLNYLIYANIEGNDWLVASVFPKEAVTGKFQNFIQTAYLTW
                     LIIGVGSAVVVAYFYFIQGKNKLQITKLAYEDEITGHYNYYRFIEYCQNINHLSKYVL
                     VNCDVKGFKWFNEIYGEEIANRLLKQIIICIDTQCQNEEFCCRQSADHFVILLDSDDF
                     EQIKNRLFVLANYIRGEFAKEYNTSPYYFHFGVYKILEDDVDINLAFKKTQYTSNDLK
                     RLSKDDVSFYQEEVFQKELYALQIEKEFQDSLKANHFKAYIQPKVNLQTGKVCSGEIL
                     TRWHHPEYNIISPGDFIPVYEKNGML"
     gene            complement(1713..2144)
                     /locus_tag="PLK68_05095"
     CDS             complement(1713..2144)
                     /locus_tag="PLK68_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89823.1"
                     /translation="MKKQYDLCCNIAQTLNIIGDKWTLLILHAVKEKKHTYKEIHESL
                     PGIPTNLLSNRLKELCQDDLLQCELYSKHPPRYRYSLTAKSIDLDDIYNALIIWGDRH
                     LDKSYKCISHDGCQGEIEIVYRCKECGEIINKEDLKIAPKE"
     gene            complement(2171..3331)
                     /locus_tag="PLK68_05100"
     CDS             complement(2171..3331)
                     /locus_tag="PLK68_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRM89824.1"
                     /translation="MATHISLALVFVEGLVSFLSPCVLPIIPIYMGYLAGNSNEKRNS
                     NKKVLLFTISFIFGILLAIFLMNASINLLQSFLKEHMTLFVRIGGILIVLLGIYQLGF
                     IKINFLQRTFRFSLKTDNKMNVMVAFIMGFTFSFAWTPCIGPALSSILLLAASSGDFW
                     YSNFLMIIYAFGFTLPFLVLGLFTNKALNWLNSHRDIVKYTTKIGAVILIIFGLMMFS
                     GKLNTISNYMSPSQGQVSTNQAENSDDSVNYGNALLNQDDKPISLADYHGKVVFLNFW
                     ATWCPPCQREMPEIQKLSEKYQNSEDIAILTVVMPGGQEMDAAGIKKFLKEKGFTMPV
                     IFDDGRLSSSFQITSLPTTYMFDRDGNVYGSVVGQLSSDMMENIIDRTLKGK"
     gene            complement(3333..3641)
                     /gene="trxA"
                     /locus_tag="PLK68_05105"
     CDS             complement(3333..3641)
                     /gene="trxA"
                     /locus_tag="PLK68_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM89825.1"
                     /translation="MSNVLYPNKQEFDELIKNEKVLVDFFATWCGPCKMIGPNIEKLA
                     EENADATVIKIDVDKHPEIAAVYGVQTIPTLIAFKKGQIVNQKIGFIPYPEILAMITS
                     "
     gene            complement(3666..>4741)
                     /locus_tag="PLK68_05110"
     CDS             complement(3666..>4741)
                     /locus_tag="PLK68_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM89826.1"
                     /translation="AKLADLQNVDVITATAKFVDDHHVAICTENEEEIIFGDLIFVNT
                     GATPVMPPIKGLATANKVYTSASLMKLEELPKTLAIIGGGYIGLEFASMYARYGSEVT
                     VYESNDRLIAREDRDIAEEIQRILEKQGVKFVFNANIQALANEGEEVLVTYNDQVSQR
                     FAGILMATGRKANTADLNLDAAGIEVNQRGEIVVNKYLQTTKKNIFALGDVKGGLQFT
                     YISLDDSRIVMDYLFGDKKRSTLNRGNIAYSVFISPTFSRIGLSEVEAKAAGYEVIVT
                     KLLTAAVPKANVLKKPEGLLKAIIDKKTERILGCVLLCEHSEELINLVTLVMNNDLSY
                     KVLKNQIFTHPTMAEALNDLFDL"
BASE COUNT         1630 a          877 c          591 g         1643 t
ORIGIN      
        1 ccaacattcc gtttttttca taaaccggga taaaatcacc aggtgaaata atattgtact
       61 caggatgatg ccaacgtgtt aaaatttcgc ctgagcaaac cttccctgtt tgaagattta
      121 ctttcggttg aatgtaagct ttaaaatgat tagcttttaa cgaatcttga aactcttttt
      181 caatttgcaa tgcataaagc tctttttgaa aaacttcttc ttgataaaag ctcacatcat
      241 cttttgataa acgttttaaa tcattactag tatattgagt cttcttgaaa gccaaattaa
      301 tgtcaacatc atcctcaaga attttataaa caccaaaatg gaaataatat ggtgaggtat
      361 tatattcttt cgcaaattca cctctaatat aattggctaa cacaaataaa cgattcttga
      421 tttgttcaaa gtcatcacta tccaataaaa ttacaaaatg atcggcagat tggcgacagc
      481 aaaattcttc attttgacat tgagtatcta tgcaaataat aatttgtttt aataatctat
      541 tagcaatctc ttctccatat atttcattaa accatttaaa tcctttaaca tcacaattta
      601 ctaaaacata tttagataaa tgattgatat tttgacaata ttcaataaac cgatagtaat
      661 tataatgacc agtaatctca tcttcataag ctaatttagt aatttgcagc ttatttttcc
      721 cttgaataaa ataaaaataa gcaactacga ctgcactgcc aaccccaatt ataagccagg
      781 taagataagc tgtttgaata aaattttgaa acttcccagt tacagcttct ttgggaaaaa
      841 cagaagcaac tagccaatca ttaccttcaa tattagcata aatcaaataa ttaagagtat
      901 tattttcatc acgaaagttt attatgccct tcccatttat atcgatatca ttaagatcaa
      961 tgccattcaa cgattcaata ttattaaaat catctgtctt cttattactt aaaacaacat
     1021 caccattttc attaaaaata aatgcatagc caacattatt ataagttgca gacgacaaaa
     1081 tacttgataa cttatctgta taaaaagtag caaacaaaac cccactaact tcattatgtt
     1141 tatatattgg tacagaataa acattgattg cctcaccatc cgtataatca gtaatacttt
     1201 tcgaggttac agttttacca tttaatgaat ctttaaaata atctcgatca gaaatattaa
     1261 aagtctgatg atcagaggtt actacatttc cttgtaaatc tgctatacca aaacgcttta
     1321 ccccatccct ttgtgccaac tggtttagat aatcaagtaa tggctcttga agaattccat
     1381 cttgttgaat aattaaactt atcgtcgtta attgggtcaa attttcattg atccggtttt
     1441 ttattgcttt agctgtctga ttggttaaat cattcaaata ctcgtcagtt tcactataca
     1501 gattatctga caagttatta atatacaaag ttgctaaagt tccaaagaga attaatagta
     1561 ttattgcaac aataattgat ttcttcatta ttgaacttct cattatcttc accactcttt
     1621 cttctattat agctaattct ataaagaaaa gcattaaaat aaaaaggcca tagcctaaat
     1681 aagttaaata aactcatttg actataacca tattattctt tgggagcaat ttttaaatct
     1741 tccttattaa taatttcacc acattcttta caacgataaa caatctcgat ttcaccttga
     1801 catccatcat ggctaataca tttataactt ttatctaaat ggcgatcacc ccagataatc
     1861 aaagcattat aaatatcatc aagatcaata cttttagctg ttaaactata tcgataacgg
     1921 ggaggatgtt ttgaatatag ttcacattgt aataaatcat cttgacagag ctccttaagt
     1981 cgatttgata ataaatttgt agggatacct ggtaatgact catgaatctc tttataagta
     2041 tgtttttttt cttttaccgc atgaagaatc aataatgtcc atttatcacc aattatattt
     2101 aatgtttgag cgatattaca acaaagatca tattgctttt tcattttatt cacctcagtt
     2161 caatcatatc ttacttgcct tttaacgttc ggtcaataat attttccatc atatcactcg
     2221 ataattgtcc aactacagag ccgtatacat tcccatcacg atcaaacata tatgttgttg
     2281 gtaagcttgt aatctgaaag ctactgctta aacgaccatc atcaaatata actggcatag
     2341 taaagccttt ttcttttaaa aactttttaa ttccagcagc atccatttcc tgacctcctg
     2401 gcattacaac tgttagaata gctatatctt cactattttg atatttttct gaaagttttt
     2461 gaatttccgg catttctctt tggcagggag ggcaccaagt tgcccaaaaa ttcaaaaata
     2521 ctacctttcc atgataatca gctaagctaa tcggtttatc atcttgattc aataaagcat
     2581 tgccataatt aaccgaatca tcactatttt cagcttggtt agtggatact tgcccttgtg
     2641 aaggtgacat ataattgcta attgtattaa gtttgccact aaacatcatc aaaccaaaga
     2701 taatcaaaat aacagcgcct attttagttg tatatttaac tatatcacga tggctattta
     2761 accagtttaa agctttattt gtaaacaatc ctaaaactag aaatggtaaa gtaaaaccaa
     2821 aagcataaat aatcattaga aaattactat accaaaaatc acctgaacta gctgctaaaa
     2881 gtaagattga tgacagcgct ggcccaatac aaggagtcca agcaaaacta aatgtaaatc
     2941 ccatgataaa cgccaccatc acattcattt tattatctgt tttcaaagaa aaacggaaag
     3001 ttcgttgtaa aaaatttatt ttgataaatc ctaactgata aattcctaat aaaacgataa
     3061 gaataccgcc gattcgaaca aataaagtca tgtgttcttt taaaaaactt tgtaataaat
     3121 ttattgacgc attcatcaga aaaattgcta aaagtatccc aaagataaag ctgattgtaa
     3181 ataataatac ttttttattt gagtttcttt tttcgttact attaccagct aaatagccca
     3241 tataaatagg aattattggt aaaacacagg gtgaaagaaa cgatactaac ccttcaacaa
     3301 aaactaatgc aagacttata tgtgttgcca ttttaacttg taatcattgc taagatttca
     3361 ggatatggaa taaatccaat tttttgattt acaatctgtc cttttttaaa ggctattagt
     3421 gtcgggattg tttgaacccc atataccgca gcaatttctg gatgtttatc aacatcaatc
     3481 tttatcactg tagcatcagc attttcttcc gctagttttt caatattagg accaatcatc
     3541 ttacatggtc cacaccatgt tgcaaaaaaa tctactaata ctttttcatt tttgattaat
     3601 tcatcaaatt cttgtttatt tggatataat acattactca tttttatttt ctcctttttt
     3661 ctattttata aatcaaacaa atcatttaac gcttcagcca ttgttggatg tgtaaagatt
     3721 tggttcttta ggaccttgta agataaatca ttattcatca ctaaagttac taaattaatt
     3781 aattcttctg aatgttcaca taacaataca caacctaaga ttcgctcagt ttttttatca
     3841 atgatcgctt tcaacaatcc ttctggcttt tttagaacat tagctttagg tactgcagct
     3901 gttaataact tagtcacaat cacttcataa ccggcagctt tagcttctac ttcacttaaa
     3961 ccaattcttg aaaaagttgg tgaaataaat accgaataag caatattacc acgatttaaa
     4021 gtagaacgct ttttatcacc aaatagataa tccataacaa ttcttgaatc atctaaagaa
     4081 atataagtaa actgaagacc accttttaca tcaccaagag caaaaatatt tttctttgtt
     4141 gtttgcagat acttattgac tacaatttca cctcgttggt taacctcaat acctgcagca
     4201 tcaagattta aatcagctgt atttgcctta cgaccagtag ccatcaatat cccggcgaag
     4261 cgttgtgaaa cttggtcatt ataagtaact agaacctctt cgccttcatt agcaagagct
     4321 tgaatattag cattaaaaac aaacttaaca ccttgttttt ctaaaattct ttgaatttct
     4381 tcagcaatat cccgatcctc acgagcaatc aaacgatcat tactttcata tactgttacc
     4441 tcgctaccat aacgtgcata catactagca aattctaaac caatatatcc accgccaatg
     4501 attgctaagg tttttggcag ttcttctaat ttcatcaaac tagcacttgt gtatactttg
     4561 ttagcagttg ctagcccctt aattggaggc ataactggag tagctccagt attaacaaag
     4621 atcaggtcac caaaaattat ttcttcttca ttttcggtac agatagctac atggtggtca
     4681 tccacaaatt tggcagttgc tgtaattaca tcaacatttt gtaaatcagc taattttgca
     4741 t
//
LOCUS       DAOMTA010000139         4722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_472_length_4722_cov_2.348618, whole genome shotgun sequence.
ACCESSION   DAOMTA010000139 DAOMTA010000000
VERSION     DAOMTA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_472_length_4722_cov_2.348618"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLK68_05115"
     CDS             complement(<1..664)
                     /locus_tag="PLK68_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRM89827.1"
                     /translation="MKAYLILEDGSVFEGTSIGAVKEVVSEFVFNTSMTGYLEVLTDP
                     SYAGQSVVMTYPLIGNYGVCLEDQESSKPHVEGFIVNELARLGSNFRKNIDLEEYLIQ
                     HDIPGIQGIDTRNLTKIIRNIGCMNGMITTTKYENLDEVMKRIHAFKVTGVVEQTTCK
                     ESYTIGTGDKRVALLDYGAKANIAKSLVKRGCQVTVYPASTPASVILKEKYDGIMLSN
                     GPG"
     gene            complement(1023..4241)
                     /gene="carB"
                     /locus_tag="PLK68_05120"
     CDS             complement(1023..4241)
                     /gene="carB"
                     /locus_tag="PLK68_05120"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM89828.1"
                     /translation="MPKREDINTVLVIGSGPIIIGQACEFDYSGTQACKALRGLGYKI
                     VLVNSNPATIMTDPETADVTYIEPLNVERLTQIIEKERPDALLPNLGGQSALNLCSEL
                     NEAGVLEKYNVQVLGVQVDAIERGEDRLEFKKEMDKLGIEMARSEVSTTVEEALAIAE
                     RLGYPVVVRPAYTMGGAGGGLVYNVEELKTVVSRGLQASLVGQCLIEESILGWEELEV
                     EVVRDANNNMITVCFIENIDPLGVHTGDSFCSAPMLTISQEVQDRLKDQAFRIVESIG
                     VIGGTNVQFAHDPETDRIVVIEINPRTSRSSALASKATGFPIALVSAMLAAGLTMEDI
                     PCGKYGTLDKYVPDGDYVVIKFARWAFEKFKGAEDKLGTQMKAVGEVMSIGKTYKEAF
                     QKAIRSLEIGRYGLGYAKNFNKLSKEELLHMLTVPTSERQFIMYEALRKGATVEELFE
                     LTQIKHYFIEQMKELVEEEENIKSYEGKELPTEVLKQAKQDGFSDKYLSQILNVEENV
                     IREERLANNINQAWEPVHVSGTKDSAYYYSTYNAEDNSDIHHDKKKIMILGGGPNRIG
                     QGIEFDYCCVHAALALKKLGFETIIVNCNPETVSTDYDTSDKLYFEPLTLEDVLSIHE
                     KEKPVGVIAQFGGQTPLNLAAQLKKNGVNILGTTPETIDMAEDRDLFNAMMEKLEIPM
                     PESGMAVNVEEALEIAKRIGYPLMVRPSYVLGGRGMEVVYDDESLEQYMKAAVGVTPD
                     RPILIDRFLNNAMECEADAISDGETVFVPAVMEHIELAGIHSGDSACILPSKHIPIRH
                     LETIKEYTKKIAKEMNVRGLMNMQYAIADDKVYVLEANPRASRTVPLVSKVCNINMVK
                     IATDIITRELTGRPSPVPTLTEKKIPHIGVKQAVFPFNMFPEVDPVLGPEMRSTGEVL
                     GIASSYGAALYKAEEGAKTILPTEGKVLISVSDLDKPEVVELAQGYYDAGFTIVATGN
                     TYNLIKESGIPVEKIKKIHEGRPNISDALTNGELAMIINTPHGKQSAHDDSYIRKAAI
                     KMRIPYMTNIAAAKASLEGIMEMKIHGSHEVKSLQEYHLAIK"
BASE COUNT         1515 a          919 c          600 g         1688 t
ORIGIN      
        1 ctccaggacc atttgaaagc atgattccat catacttttc ttttagaata actgacgctg
       61 gcgtactggc aggatagact gtaacttgac atccacgttt cactaaactt ttagcaatgt
      121 tagcttttgc tccataatct aataaagcaa ctcttttatc tccagtacca attgtataag
      181 actctttaca tgtagtttgt tccacaactc cagttacttt gaaagcatga atccgtttca
      241 ttacttcatc aagattttca tatttagttg ttgttatcat tccattcata catccaatat
      301 tacgaataat tttcgttaga tttcgagtat caattccttg aatcccagga atatcatgtt
      361 ggattaggta ttcctctaaa tcaatatttt ttctaaaatt acttcccaat ctagctagtt
      421 catttacaat aaatccctct acatgtggtt tacttgactc ttggtcttct aaacaaaccc
      481 cataattccc aattaatgga taagtcatta cgactgactg accagcataa gaaggatctg
      541 ttaacacttc taaatatcct gtcatcgaag tattaaatac aaactcacta actacttctt
      601 taactgcacc aatacttgtt ccttcaaata cactgccatc ttctaaaatt aagtatgctt
      661 tcatcattca tcctccttaa ttactataaa aaaacatatc aatataactg atatgtttaa
      721 taacaataaa aatataaaaa tgagttcata atatcaaatt tttcacttac taattcttta
      781 aaaaacacca acatatgcct cattcctttc ttattcttgt atagtatagt agaaatcatt
      841 tgaatattca agatattttt atactttttt attatcacga gtcaaattta ttgatataga
      901 ttaatatttt caagcctaat aataaaaaat cattgataca aacacaataa aatcccacct
      961 ccatagattt agtgaaatta tctcatttat ctactttggt gggaatagct taaatttaaa
     1021 ttttatttaa ttgctaaatg atactcttgt aaagatttaa cttcatgact accatgaatt
     1081 ttcatttcca taattccttc taatgatgct ttagcagcag caatattagt catatatgga
     1141 attctcattt taattgcagc tttacgaata taactatcat catgagcact ttgtttacca
     1201 tgaggtgtat taatgatcat tgctaattca ccattagtta aagcatcact aatatttgga
     1261 cgaccttcat gaattttttt gattttttct actggaatac cactttcttt gatcaagtta
     1321 taagtattcc ctgttgctac aatagtaaat ccagcatcat aatatccttg tgcaagctca
     1381 acaacttctg gtttgtctaa gtcactaaca ctgatcaaaa cttttccttc agttggtaaa
     1441 attgttttcg ccccttcttc agctttatat aaagctgctc catatgaact ggcaattcct
     1501 aatacctctc cagttgaacg catttcaggt cctaaaacag gatctacttc cgggaacata
     1561 ttgaatggga aaactgcttg tttaactcca atatgaggaa ttttcttttc tgttaaagtt
     1621 ggtactggtg aaggacgccc agtcaattca cgagtaataa tatctgtcgc aatttttacc
     1681 atattaatat tacatacctt tgaaactagt ggtactgttc ttgaagctct tggattggct
     1741 tctaatacat atactttatc atcagcaatt gcatattgca tattcattaa accacgaaca
     1801 ttcatttctt tagcaatttt ctttgtatat tctttaattg tttctaaatg acggatagga
     1861 atatgtttag agggtaagat acatgctgaa tcacctgagt ggataccagc taattcgata
     1921 tgttccataa ccgcaggaac aaaaacagtt tcaccgtcac tgattgcatc agcttcacat
     1981 tccatagcat tatttaagaa acggtcgatt aagattgggc gatcaggagt taccccaaca
     2041 gctgctttca tatattgttc aagactttca tcatcataaa caacttccat tccacgacca
     2101 cctaaaacat aagaaggtct taccattaat ggatagccga ttcttttagc aatttctaat
     2161 gcttcttcaa catttactgc cattccagat tcaggcattg gaatttctaa tttttccatc
     2221 attgcattaa ataagtcacg atcttctgcc atatcaatag tttctggtgt tgttcctaaa
     2281 atattcacac catttttctt taattgagca gctaagttaa gtggtgtctg tccaccaaat
     2341 tgtgcaatta cacctactgg tttttctttt tcatgaatac ttaaaacatc ttctaatgtt
     2401 agtggttcaa agtataattt atcagatgta tcatagtcag ttgatacagt ttcagggtta
     2461 cagttaacaa taattgtttc aaatcctaat ttctttaatg ctaaagcagc atgaacacag
     2521 caatagtcaa attcaattcc ttgtccaata cgattaggtc caccacctaa aatcataatt
     2581 ttctttttat cgtgatgaat gtcgctatta tcttcagcat tatatgttga ataataataa
     2641 gcactatcct ttgttccact tacgtgtact ggttcccatg cttgattaat attattagct
     2701 aatctttctt ctctaattac attttcctca acattcaaaa tttgacttaa atatttatct
     2761 gagaatccat cttgttttgc ttgttttagt acttcagtcg gtaattcttt accttcgtat
     2821 gattttatat tttcttcttc ttcaactaat tctttcattt gttcaatgaa ataatgtttg
     2881 atttgtgtta gttcaaataa ttcttcaact gttgctccct ttcttaacgc ttcatacata
     2941 atgaattgtc tttcgctagt tggaacagtt aacatatgta ataattcttc tttagataac
     3001 ttattgaagt ttttagcata ccctaagcca taacgtccaa tttccaatga acgaattgct
     3061 ttttggaaag cttctttata agttttgccg atactcataa cttccccaac agctttcatt
     3121 tgagtaccaa gtttatcttc agcccctttg aatttttcaa atgcccaacg tgcaaacttg
     3181 attacaacat aatcaccatc aggtacatat ttatctaaag ttccatattt accacaagga
     3241 atatcttcca tagtcaaacc agccgctaac attgctgaaa ctaaagcaat tgggaatcct
     3301 gttgcttttg atgctaaagc tgatgaacga gatgttcttg gattaatttc aataacaacg
     3361 attcgatcag tttctggatc atgagcaaat tgaacatttg tccctccaat tacaccaatt
     3421 gattcaacaa ttctaaatgc ttggtctttt aaacgatctt gcacttcttg gctaattgtt
     3481 aacattggag cgctacagaa tgaatctcca gtatgaactc ctaatggatc aatattttca
     3541 atgaaacata ctgtgatcat gttgttgtta gcatcacgaa caacttcaac ttctaattct
     3601 tcccaaccta aaatagattc ttcgattaaa cattgaccaa ctaaactagc ttgtaacccc
     3661 cgagatacaa ctgtttttaa ttcttcaacg ttatatacca gtccaccacc ggcaccaccc
     3721 atagtataag cagggcgcac aactactgga taacctaacc gttcagcaat tgctaaggct
     3781 tcttcaacag ttgttgaaac ctcactacga gccatttcaa tgcctaattt atccatttct
     3841 tttttaaatt ctaaacggtc ttcaccacgt tcaatcgcat caacttgaac tcctaatact
     3901 tgaacgttat atttttctaa aacaccagcc tcatttaatt cagaacataa gtttaatgct
     3961 gattgcccac ctaagttagg aagtaacgca tctggtcttt ctttttcaat gatctgtgtt
     4021 aatcgttcta catttaatgg ttcaatataa gtaacatcag cagtttccgg atctgtcatg
     4081 atcgttgcag gatttgagtt cactaacacg atcttataac ctaaccctct taaagcttta
     4141 cacgcttgag ttcctgaata atcaaattca caagcttgac caataataat tggacctgaa
     4201 ccaattacta atactgtatt aatatcttct ctttttggca tttttgttcc tccgtataat
     4261 cttgattaat ttcacaaaaa aacatatcaa gtaaatgata tgttaattat aaaaaataga
     4321 aatataaaag aattgaaaat gatcagtaat agtcttaaca atgcgttcac caaatacttt
     4381 aaacatcata atcactcctt cataacttta tccatattag tatacaaaac taaatgacac
     4441 tattcaacaa atttataatt agttatcatt gtaagaaacc ttcttcaaaa tccatatttt
     4501 tatttcaaat ctagttattg cgtttaaaac aaaacaagct gtaaatacta tataaaagga
     4561 tggttttaat gaaaaattta acacacacta aaataataaa tgaaatcatt gaaaatgttc
     4621 gtgatgatat gcaggatgaa gaaataattc atcttgttct tgaaaaaaca gttgctttac
     4681 ctgatggtaa ggatgaaaca ctaggacaaa aaattgctga ta
//
LOCUS       DAOMTA010000140         4721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_473_length_4721_cov_2.502572, whole genome shotgun sequence.
ACCESSION   DAOMTA010000140 DAOMTA010000000
VERSION     DAOMTA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_473_length_4721_cov_2.502572"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLK68_05125"
     CDS             <1..234
                     /locus_tag="PLK68_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89829.1"
                     /translation="KANKIYRIGSNSILVENNYYIFQLTHFPSNLYTPINKSHRFSVK
                     IEINNLQKYIKHNKNMIKSVLFDFIKGVLLRYT"
     gene            604..1782
                     /locus_tag="PLK68_05130"
     CDS             604..1782
                     /locus_tag="PLK68_05130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRM89830.1"
                     /translation="MNNNYELGKVIVALNINITSKDVLDYYIDLVLDGIDYEKAEIIF
                     HSKNTESLNEGTNIVLIYLKDKNPHAVTNAVAKLSSSPYIVYAEPDYIEEMHIISNDP
                     LYNQLWGIQKINAPLAWDYTTGDSSISVGVIDTGIDQNHPDIRENMWTTWNGRLIYGW
                     NFADNSSDSMDLDGHGSHVAGTIGAVGNNRIGITGVCWQVRVAALKFGLDVASAIAAI
                     DFANYYKISILNASWGGRAYSQALKDAIDQYDGLFVASAGNDGTNNDVDPMYPASYDC
                     KNIISVAAVDPYDTLARFSNYGLKTVDIAAPGTNILSLDLAGEYSPLNGTSMAAPHVA
                     GAAALLKSSMPNISTITLKRIILSSAMENPELKGKILTGGILDMETMFKLANCWHGRE
                     "
     gene            1885..2778
                     /locus_tag="PLK68_05135"
     CDS             1885..2778
                     /locus_tag="PLK68_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM89831.1"
                     /translation="MYNPQLETFIKVVDRGSFSKAAEDLFISPSAVIKQINILETNLG
                     LKLLKRTHQGVSLTPAGASLYRDAKYLIKYAKDSIVRAHKAMEKEEMVIRIGTSNTTP
                     SQFLIDLWPRIHELCPNLRIQLIPFENTPENAREILANLGDNIDLVAGVFEKDNFKQR
                     GCDSLEIKKESICLALSIYDELSNKDKLSLQDIHGRELMLIKRGWNKYVDEMRDDLIK
                     NDPTIIIKEFEFFDISVFNECENNKSLLMSVKSWKNIHPLIKTIPVEWDYYMPYGLLF
                     SLEPSRQVIEFVNAVKRVLDI"
     gene            2996..3852
                     /locus_tag="PLK68_05140"
                     /pseudo
     CDS             2996..3852
                     /locus_tag="PLK68_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            4019..>4721
                     /locus_tag="PLK68_05145"
     CDS             4019..>4721
                     /locus_tag="PLK68_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM89832.1"
                     /translation="MMPYILILVILTLLWLGAFWRIKRKQNINLLAGFLFFMFLSTFA
                     SFLVMLGINYSNRLVYLLVGVIFLFALFVLLFGVYILIAFLLINSVIILRKERFSLSH
                     ILTLLVAVGIILLITLSSFLGRINPPLPIMALWSGLIVMILFLTFHIFLFIETLILTN
                     LFHPRKNLNYIIVLGSGLINGNVSPLLAKRIQAALKFAKKQEKKKGFAPYLLMSGGQG
                     SDETRAEALAMKEYAI"
BASE COUNT         1696 a          568 c          892 g         1565 t
ORIGIN      
        1 aaggcaaata aaatttatag gatagggagt aattctattc ttgttgagaa taattattat
       61 atatttcaac tgacacactt tccatctaat ctttatacgc ccataaataa aagtcataga
      121 ttttccgtaa aaatagaaat aaataatctt caaaaatata ttaaacataa taaaaatatg
      181 attaaatcag tactttttga ttttataaaa ggtgtactat tacgttacac atgaagaaaa
      241 aatagataaa caatcaggca agatgtaata aggatattgt tttatttagt acacaagttc
      301 tcaatattaa aaaaggtaag ttgaattgaa atttattttt tttagtacgt ataattagtt
      361 gaaaaaatta acgacaggag aaaatatatg aaattataaa aaatataaag ataatttgat
      421 taagttttgg attgacactt tcacatgtta agagtcaatt ttttaattac catagtttag
      481 caaacaaaat tcactttatt cctgctatac agttttattt tcaacaaagc gctgcaataa
      541 atatagtaat gaattggaaa tagtatcata aaatataaag tatataggta aggaggaagt
      601 aaaatgaata ataattatga gcttggaaaa gtaattgtag cattaaatat aaatattact
      661 tctaaagatg tactagacta ttatattgat cttgttttag atgggattga ttatgaaaaa
      721 gcagaaatca tcttccattc aaagaatact gaaagtctaa atgagggaac taatatagtt
      781 cttatttatt taaaagataa aaatcctcat gcagttacta atgcagttgc aaaattatca
      841 tcaagtccat atattgttta tgcagaaccg gattatattg aagaaatgca tattatctca
      901 aatgatcctt tatacaatca gctttgggga attcaaaaaa tcaatgcacc gcttgcatgg
      961 gattatacaa ctggtgatag tagtatcagt gttggagtga ttgatacagg aatagatcaa
     1021 aatcatccgg acataagaga aaatatgtgg actacgtgga atggaagatt aatttatggc
     1081 tggaactttg cagataatag tagtgattca atggatctag atggtcatgg tagtcatgtt
     1141 gcagggacga ttggtgcagt tggaaataat cgtattggta ttactggagt atgctggcaa
     1201 gtaagagtag cagctttaaa atttggtctt gatgttgctt cggcaattgc ggcaatagat
     1261 tttgcaaatt attataagat ttctatttta aatgccagtt ggggtggacg agcttattca
     1321 caagctttaa aagatgccat tgatcagtat gatggcttat ttgttgcttc ggctggaaat
     1381 gatggaacta acaatgatgt tgatccaatg tatccagcat catatgattg taaaaatatt
     1441 atttctgtag cagcggtcga tccctatgat actttagccc gtttttcaaa ttatggtctt
     1501 aaaactgttg atatagcagc ccctggaaca aacattctca gtcttgacct tgctggtgag
     1561 tatagcccct tgaatggaac ttcaatggca gccccacatg tagcaggagc agcagcgtta
     1621 ttaaaatcaa gtatgccgaa tatctcaaca ataactttaa aaagaattat tttatcaagt
     1681 gcgatggaaa acccagaact taagggaaag atattaacgg gaggaatatt agatatggaa
     1741 acaatgttta aactagctaa ctgctggcat ggacgagaat aacaatatat atctaataaa
     1801 aatgtgatga ataagaaaaa taggttataa aaacatttta actattcttt aaataattta
     1861 aaatagaata aaggaagtga gaatatgtat aaccctcaat tagaaacatt tataaaggtt
     1921 gttgatcgcg gtagtttctc taaagctgct gaggacttat ttattagtcc tagtgctgta
     1981 attaaacaga taaatattct agagacaaat ttgggtttaa aacttctaaa aagaactcat
     2041 caaggggtat ctttgacacc agcaggagca tctttataca gggatgccaa atatttaatt
     2101 aaatatgcaa aggattcaat tgtacgagca cacaaggcga tggaaaaaga agaaatggta
     2161 ataagaattg gaacatcaaa tacaacacca agtcaatttc ttatcgattt atggccgcgg
     2221 attcatgaat tatgtcctaa tttaagaatt caattaattc catttgaaaa tactccggaa
     2281 aatgctcgag aaatactagc taatttaggc gataatattg atttggttgc tggtgtattt
     2341 gaaaaggata atttcaaaca gcggggctgt gattctctag aaataaaaaa agaatcaatt
     2401 tgtcttgctt tatctattta tgatgaatta agtaacaaag ataagctgtc tttgcaagat
     2461 attcatggac gtgaattaat gctgattaaa agaggctgga ataaatatgt tgatgaaatg
     2521 cgtgatgatt taattaaaaa tgatcctact attataatca aagagtttga gttttttgat
     2581 atctctgttt ttaatgaatg cgaaaataat aaaagtttgc ttatgagtgt caagagctgg
     2641 aaaaatattc atcctttaat taaaactatt ccagttgaat gggattatta tatgccttat
     2701 ggtttattgt tttcattaga gccttcacgg caagttattg agtttgttaa tgcagtaaaa
     2761 agagttttag atatatagta tcgatataac cctaaggtta tatcgatact acttattgag
     2821 acttctatgt ttataaataa ttatatatta taaagatgca aaagaaatat aaatatagga
     2881 gaagataaaa atgagaaaaa cgagaatatt aggtcaagga ttagaagtat ctgctattgg
     2941 gcttggatgt atgggaatgg atcatgctta tggggctcct gctgatcgtg aagagatgat
     3001 taaattaatt agacatgcgg ttactttagg atgcaatttt tttgatacag ctgttgtata
     3061 tggtgaagca aatgaagttt tattaggtaa agcattggag attttccctc gagatgaagt
     3121 aattattgct acaaaatttg gtatttatgg acaagaaatc gttgatggta aaccacaaaa
     3181 tattttaaat agtaagccgg attcgattag agaacaatta gagggatcgt taaaacgact
     3241 aggcgttgat tatattgatc tatattatca acatcgagtt gatcctgaag tagaaccaga
     3301 aatagtggct agcgtgatga aagagttaat agctaaagga aaaataaaac attggggttt
     3361 atcaaatgca ccattagatt atttaaaaag agcacatgcg gtatgtccag taacttgtat
     3421 tgaaaatcag tggtctttag acagcctgaa aaggaagtgt ttaaagtttg tgaggagtta
     3481 aatgtaggat ttgttgctta ttctccacta ggaaacggat ttttatcagg taaatatacg
     3541 ccagcaacaa agtatgctga aggtgatttt agaaataata tgggacgttt taatcctgaa
     3601 gtaatgaaac gtaatcaggc cttattagat ttagtgcaag aaattgctga acgtaaaaat
     3661 gctactagcg cacaaattgt tctagcttgg gagattaatc aaaaagattg gattgttcct
     3721 attccaggaa caaccaaaat acatcgtttg gaagaaaatt taggagcaat ggaagttgag
     3781 ttgactgaac aggagatggc ggctattaat caagcattag acaatcttga tattgatgaa
     3841 acacattttt aagcagagaa aagtacaaag gaatttgtac tttttagtgt tatacgttta
     3901 atcctgaata atgaataata tagaaactat cacggtgtat ttagttttga aaaagtgtat
     3961 tatttggtat atcggcgtta ttagtctata atagagttga aagaaagccg gtggtaaaat
     4021 gatgccctat atattgatct tggttattct aacactttta tggttaggcg cattttggag
     4081 gataaaaaga aagcaaaata taaacttact agctggcttt ttatttttca tgtttttaag
     4141 tacctttgct agttttttag taatgttagg aattaattat tcaaatagat tagtatattt
     4201 attagttggt gtaatttttt tatttgcctt gtttgtttta ttatttggag tctatatctt
     4261 aattgcattc ttattaataa atagtgttat cattcttcga aaagagaggt tcagtctttc
     4321 gcatattttg actttgttgg tagcggtagg gataatttta ctgattacac tatcatcatt
     4381 tttaggtagg attaatccac cattaccaat tatggcattg tggagtggcc tgatcgtaat
     4441 gatcttattt cttacttttc atattttctt atttatagaa actttgatat taacaaatct
     4501 ttttcatcct agaaaaaatt taaattatat cattgtttta ggaagtggac taattaatgg
     4561 aaatgtttct cctttattag caaagcgtat acaagcggct ttaaagtttg ctaaaaagca
     4621 agaaaagaaa aaaggatttg caccttattt gttgatgtcc ggaggacaag gaagtgatga
     4681 aacgagagcc gaagctctag caatgaaaga gtatgcgatt g
//
LOCUS       DAOMTA010000141         4719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_475_length_4719_cov_2.018439, whole genome shotgun sequence.
ACCESSION   DAOMTA010000141 DAOMTA010000000
VERSION     DAOMTA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_475_length_4719_cov_2.018439"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLK68_05150"
     CDS             complement(<1..243)
                     /locus_tag="PLK68_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM89833.1"
                     /translation="MKINEIIKEKRLALGYTQEQLAKFLNLTTPAVNKWERGISYPDI
                     TILPALARILKTDLNTLLSFKDDLSDYEVVLFLNDLA"
     gene            complement(342..>1186)
                     /locus_tag="PLK68_05155"
     CDS             complement(342..>1186)
                     /locus_tag="PLK68_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89834.1"
                     /translation="SIINIVLDLIFITQFDMGAVGAAYATVIAQFLSGAACYIYMKKK
                     TDVLTFEHDDKKFSKKHSIRLLQMGLPMAMQFSITAIGSVVIQSAVNTLGSDVVAAVT
                     AAIKISVMLTQPLETLGLTMATYGGQNLGANKIGRIFAGLRVSCIIGAAYCAIVFIFV
                     YFTSDYLSLLFIDAKEVVIMAEIKQYLLINSMGYYILCILFILRNLLQGLGYSFLAMF
                     GGVAEMIARCIVAFFFVSSFGFNAICFANPLAWLFANIVFIGGWIYKRKELKVIQSSE
                     KVTV"
     assembly_gap    1187..1196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1197..1744)
                     /locus_tag="PLK68_05160"
     CDS             complement(<1197..1744)
                     /locus_tag="PLK68_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89835.1"
                     /translation="MKKDMTTGSPLKLIIAFGIPLVIGNIFQQFYSMVDTIIVGKYVG
                     KTALAAVGSTGSLNFMIIGFGIGICSGFGIPIAQSFGGKKIQDMKKYIVNSFYLCMLI
                     TAIMTIATVIALPSVLELMQTPSDIYQQAYDYIVVIFIGLFATMLYNILSSILRAIGD
                     SRTPLYFLILSSETTLRFNTTHR"
     gene            2044..2193
                     /locus_tag="PLK68_05165"
     CDS             2044..2193
                     /locus_tag="PLK68_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM89836.1"
                     /translation="MVILGANGAGNSILMKLLTGAYLPEYGQIILHSNIKVAHLNQYA
                     TLNQE"
     gene            2300..2542
                     /locus_tag="PLK68_05170"
     CDS             2300..2542
                     /locus_tag="PLK68_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM89837.1"
                     /translation="MTLAGRYQEKLINSGYYTIDTSINMVTAGLGINLIGLDKKINKI
                     SGGQCAKVILAKLLLEKLDVLLLDELTNFLDTKHIN"
     gene            3288..3482
                     /locus_tag="PLK68_05175"
     CDS             3288..3482
                     /locus_tag="PLK68_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89838.1"
                     /translation="MYVAIITSHLLILDEPTNHLDQEIKKALAKAIKNYSSSAILVCH
                     EPDFYQELVDHIIKIENCNF"
     gene            complement(3620..4600)
                     /locus_tag="PLK68_05180"
     CDS             complement(3620..4600)
                     /locus_tag="PLK68_05180"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HRM89839.1"
                     /translation="MIKIGIVGYGNLGRGVECAVLHSQDMELTGVFTRRNPKSVKTHS
                     DVPVYSMDKVYDMKDQIDVLVLCGGSANDLPKQTVELAEYFNVVDSFDTHAKIPEHFA
                     NVDEASKKNKHISIISVGWDPGLFSLNRLYGQAILPQGHDYTFWGKGVSQGHSDAIRR
                     IDEVKDARQYTIPVEAALQSVRNGENPTLVTRQKHTRECFVVAEEGADLQRIEEEIKT
                     MPNYFADYDTTVHFISEAELLRDHQGIPHGGVVLRSGTTGFEQENKHVIEYKLTLDSN
                     PEFTSSVLVAYARAAHRMYQEGQHGCKTVFDIAPAYLHPESGDELRKKLL"
BASE COUNT         1715 a          801 c          596 g         1597 t
ORIGIN      
        1 agctaaatcg tttaaaaaca acacaacttc ataatctgac aaatcatctt taaatgacaa
       61 tagagtattt aagtctgttt ttaggattct tgctagagct ggtaaaatag taatatctgg
      121 ataactaatc ccccgctccc atttattgac tgctggcgta gtaagattta aaaacttggc
      181 taattgctct tgtgtatacc ctaaagctaa tcgcttttct ttaattattt cattaatttt
      241 cattaatttt cacctcatta tagtaatata ataacatatg cattctaaga tacaattgac
      301 cgttagttac tattgctggg aaaaaataaa cgaattgtta attaaactgt tactttctcg
      361 ctactttgaa ttacttttaa ttctttacgt ttataaatcc atccaccaat aaagacaata
      421 ttcgcaaaaa gccacgctaa tggatttgca aaacaaattg cattaaaacc aaatgatgaa
      481 acaaaaaaga aagcaacaat acagcgagct atcatttctg caacaccgcc aaacattgct
      541 aaaaaactat aacctaatcc ctgtagtaaa ttacgcaata taaataaaat acataagata
      601 taatatccca tcgaattaat taacaaatat tgtttaattt ctgccataat aacaacttct
      661 ttagcatcga taaataacaa tgatagataa tcagaagtaa agtaaacaaa tataaataca
      721 atagcacaat aagcagcgcc aataatacaa cttactcgta atccggcgaa aatccgtcct
      781 attttatttg cacctaaatt ctgaccgcca tacgttgcca ttgttaaccc taaagtttct
      841 aatggctgag tcaacataac agaaatttta atcgcagcag taactgcagc tacaacatct
      901 gaacctaaag tattaacagc agattgaatc acaacactgc caattgcagt aatcgaaaac
      961 tgcattgcca ttggtaagcc catttgtaat aatcgaatac tgtgtttttt actgaatttt
     1021 ttatcatcat gttcgaaagt taaaacgtcg gttttctttt tcatataaat atagcaggct
     1081 gctccagata gaaattgggc aattactgtt gcataggcag ctcctactgc tcccatatca
     1141 aattgagtaa taaaaatcaa atctagaaca atattaatta ttgatgnnnn nnnnnncggt
     1201 gtgtggtatt gaaccttaag gtagtttccg atgataaaat caaaaaatat aacggtgtcc
     1261 ttgaatctcc gattgctctt aaaatacttg ataaaatatt atatagcatt gtggcaaata
     1321 atccaataaa gataaccacg atataatcat atgcctgctg ataaatatca cttggtgttt
     1381 gcatcaactc taaaactgat ggtaaagcaa taacagtagc aattgtcata attgctgtaa
     1441 tcaacataca taaataaaat gaattaacta tgtatttttt catgtcttgt atttttttac
     1501 caccaaaact ttgggcaatt ggaattccaa aaccactaca tataccaatt ccaaaaccaa
     1561 taatcataaa gtttagtgat ccggtcgacc ccacagctgc taaagctgtt ttcccaacat
     1621 atttcccaac aataattgta tcaaccatac tataaaactg ttgaaaaata ttacctatta
     1681 ctaaaggtat cccaaaagca ataatcagct taagaggact acctgtcgtc atatcctttt
     1741 tcatcatttt ccctccttaa acatgtttat ttattataca ttattttcta acaatgttaa
     1801 gaaaatttat aaatatgaaa aactatttat atcaagtaat cactttatta aaaaatatag
     1861 tttaatacat ttattgactg aaattgacag tatctttaat ttattatata ttcatttgca
     1921 gaaggtaata tttatgttaa tatttactgc tttattagat aggaaaaaaa gaaaatgagt
     1981 ttattaacaa ttaaagattt atctcatgat tttagagata aaatatgata taaaaatgaa
     2041 catatagtca tcttaggagc caatggtgct ggtaatagca tattaatgaa attgcttacc
     2101 ggtgcttatt taccagagta tggtcaaatt attttgcatt ctaatattaa agtcgcccac
     2161 cttaatcaat atgctactct taatcaagaa taaataatca aagaatatct tagtcctgtc
     2221 tacaatgaag aatatttttt agaacaaaaa atgctaacac tgtatcaaaa aaagaagttt
     2281 taataataat gaagaacttt tgactttagc tggtagatat caagaaaaat tgattaacag
     2341 tggttattat actatcgata cttcaataaa tatggtaact gcaggtcttg ggattaatct
     2401 aattggactt gataaaaaaa ttaataaaat cagtggtgga caatgtgcta aagtgattct
     2461 agctaaattg ttattagaaa aactagatgt tttattattg gatgaactta ctaatttttt
     2521 agacactaaa catattaatt agcgagttga ctttttaaat aattttatgg gcacctatat
     2581 tgttatctcg cacgattttg attttctaga taaaattact gattgtattt taaatattga
     2641 ttattataca ataaaaaata tcgtggaaaa tattcatctt tttaaaacaa aaaaacgtct
     2701 tcgcactgat tatctaagac gttatcaaaa acaacaaaaa catataaaaa acggaacaat
     2761 ttatccgcaa aaatatagct gggaataatt cagaaataac tcaaggaaga cgtaaacaat
     2821 tagctcactt tgaacgtcta acatcaccta ttacaattat aatactagat tttaaattta
     2881 aaaatctacc acttttaacg aaacaaattc ttaaagcagc taatttaacg attggatatc
     2941 ataagccttt actttcaaag atcagctttt cactgtccgc tcaagaaaaa ttgccattac
     3001 tggatttaac agtgttggaa aatcaacact taaaaaacgt tactaggtga aattattctg
     3061 atatcaggaa cctttatttt taatgatcaa ataaaaattg cttattataa tcaagattta
     3121 atttggacta atgattatca aacaccgtta aaaatagttc acgatagtca tcctaaatta
     3181 ggcattaaga caattcgaac caatcttgca catttcaata ttacgaccga tcttattgat
     3241 aaaccaatta tccaattaaa tggtggtgag cagacaaaag taaaattatg tatgttgcta
     3301 ttattactag ccatttattg attttagatg aaccaactaa tcatctagat caagaaataa
     3361 aaaaagcatt ggcaaaagca attaaaaatt attctagtag tgctatttta gtttgccatg
     3421 agcctgattt ttatcaagaa ttagttgatc acataattaa aatagaaaac tgtaatttct
     3481 aataaaaacc tacatagtag ttaatgatct gatgcctctt taatattcag cgtttcattt
     3541 atttacacag tctactttag aagtaccata ccataacatc tactagtagg ttttagattt
     3601 attaaatttg aaaattttac tataataatt tttttcttaa ttcatcacca gattcaggat
     3661 gtaagtaagc aggcgctata tcaaaaacag ttttacatcc atgttgaccc tcttgataca
     3721 tccgatgcgc tgctcgagca tatgcaacta atacacttga cgtgaattcc ggatttgaat
     3781 ctaaggttaa tttatactca attacatgtt tgttttcttg ttcgaatcca gttgtaccac
     3841 ttcttaatac aacccctcca tgaggaattc cttggtgatc acgtaataat tcagcttcac
     3901 ttataaaatg aacagtagta tcatagtctg caaaataatt aggcattgtc ttaatttctt
     3961 cttctattct ttgtaaatca gccccttctt cagcaacaac aaagcattca cgagtatgtt
     4021 tttgacgtgt tactaaagtt ggattctccc cattacgaac actttgaaga gcagcttcta
     4081 ctggaatagt atactgtctt gcatccttaa cttcgtcaat tcttctgatt gcatcagaat
     4141 gtccctgact aacccctttt ccccaaaaag tataatcatg accctgcggt aaaatagctt
     4201 gtccataaag acggtttaat gaaaataacc ctggatccca gccaacagaa ataatactta
     4261 tatgtttatt ctttttactt gcctcatcaa cattagcaaa atgttcaggg attttagcat
     4321 gtgtatcaaa actatcaact acattaaaat attctgctaa ttctactgtt tgttttggta
     4381 aatcattggc actaccacca cataaaacaa gaacatcaat ttgatctttc atatcataaa
     4441 ctttatccat actataaaca ggtacatcac tatgtgtttt tactgattta ggatttcttc
     4501 ttgtaaaaac cccagttagt tccatatctt gactatgtaa tactgcacat tctaccccac
     4561 gtcctaaatt tccgtatcct acaattccta tttttatcat gtttccactc cttatatttt
     4621 ttaatatttt atcattcttt tatcaaaaaa acacgaatat gaaatttttc ttacatcaat
     4681 atattttttt ctcatattta aatggttttg aaagtagaa
//
LOCUS       DAOMTA010000142         4700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_477_length_4700_cov_2.433369, whole genome shotgun sequence.
ACCESSION   DAOMTA010000142 DAOMTA010000000
VERSION     DAOMTA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_4700_cov_2.433369"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLK68_05185"
     CDS             complement(<1..612)
                     /locus_tag="PLK68_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89840.1"
                     /translation="MKTDTETLRKRGFLTNTEAEPYFLYSKEELLELLKDKTAVNRTA
                     ALFILRSFVDINELDEILLRMLVKEKALYTKLEICDILTTGNELTIKRMIPYMGTIRG
                     NQHRTIPEKVSKKKSYPLPRDIIARTMAKMNPDYFSTILEIINYPEDKVVAEAIDAIG
                     WMVFYHQELATAKNYQTVIQSFERYHDNELMKWKLIICLSAFNQ"
     gene            complement(700..2397)
                     /locus_tag="PLK68_05190"
     CDS             complement(700..2397)
                     /locus_tag="PLK68_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM89841.1"
                     /translation="MVVNKTIFRYFLTVLLVTLVLSSSVSMVILSSQMLENTKHDMLY
                     AVKLVDYQLDESHDLKAQVDALNPLAYNDQTRLTVIDTNGEVLADSGSEEIDENHKGR
                     EEVKQALSEGVGYATRYSSTVKRNMLYVAVFNKGYIVRLALPYNGIFDNLPTLVRPLG
                     VGAIMSLVIALFLSKRFANTLTAPIQDITTQVTKMKDYRELEFDSYKYDEFNIIASKL
                     EEQAKTIHDTMKKLKSEQIKINGILDQMKEGFVLLDSDLTVLMVNRKAQKLYGHTIKL
                     NCSIKDFIFDFKIINALDHLSDEQQVVEVEKEKEFYNCYVAKVDYGVTLLFVNITEQH
                     NAMKMRQEFFSNVSHELKTPMTSIRGYSELLETGVINDKDASKKALDKIHDEVNNMST
                     LINDILMISRLENKDVDVIKHPVHLTPLVDEIIDTMQVEIDKKHLQVDKELEDITYTS
                     NHQHMHQLLSNLITNAIKYNVDGGKIIIKSYQFGRNIIIEVSDTGRGISKIDQGRVFE
                     RFFRCDQGRDKETGGTGLGLAIVKHIVQYYQGNITLTSKLHEGTTFKVTLPMEEEVI"
     gene            complement(2384..3067)
                     /locus_tag="PLK68_05195"
     CDS             complement(2384..3067)
                     /locus_tag="PLK68_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM89842.1"
                     /translation="MGERIFVVEDDENIREIINLALVSNGYEVVQFDNAIDALAEIDK
                     KAPSLAIFDLMLPKMSGIDAIKEIRETDSELPILILSAKDREIDKVNGLDSGSDDYMT
                     KPFGILELQARVRSLLRRHVTSDIIRTKHLTVDKQTRLVKLDDQKLELTNKEYQLLVY
                     LMDNKHRVVEREELLNEIWGYDFIGESRALDVHIRALRSKLNDDGHKYIKTIRSVGYR
                     FYEEGDGSE"
     gene            complement(3080..3739)
                     /gene="phoU"
                     /locus_tag="PLK68_05200"
     CDS             complement(3080..3739)
                     /gene="phoU"
                     /locus_tag="PLK68_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRM89843.1"
                     /translation="MLRTNFENDLNKLHVDLDKMCHLVILAIENCIVAFKSGDRELCR
                     DILAGDKVINDMERTIEARCLSLILKQQPVASDLRNVSTALKVVTDLERIGDQGADIA
                     EILLDTDVTCPYKMVEHIPNMAHLAKNMVKQSIEAFHQHDLKKASEVKKLEDDMDGLF
                     EEVKVELIQIVNESKETIDLAINFLMIAKYFERIGDHAVDICEWVEFNQTGTVDNYRL
                     I"
     gene            complement(3749..4504)
                     /gene="pstB"
                     /locus_tag="PLK68_05205"
     CDS             complement(3749..4504)
                     /gene="pstB"
                     /locus_tag="PLK68_05205"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRM89844.1"
                     /translation="MENKIEAKNLDLYYGEKHALKNVSLDIKTNKITAFIGPSGCGKS
                     TFLKTLNRMNDYVKDIKITGSVVLDGEDIYDSRVDTTVLRKKVGMVFQQPNPFPMSIY
                     DNIAYGPRIHGIKNKKQLDKIVKDSLEAAALYEEVKDRLHSSALGLSGGQQQRLCIAR
                     ALAVEPDVILLDEPTSALDPISTLKIEELLMELKEKYTIAIVTHNMQQASRIADYTAF
                     FLVGEMVEYGKTKDVFAMPKDKRTEDYITGRFG"
     gene            complement(4506..>4700)
                     /locus_tag="PLK68_05210"
     CDS             complement(4506..>4700)
                     /locus_tag="PLK68_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HRM89845.1"
                     /translation="VLPKNIFTHLSSSGATLTIQLYLEMAKANYESAFVIALVLIVIV
                     LGLNMLAKLITNKFDVNRVD"
BASE COUNT         1523 a          910 c          558 g         1709 t
ORIGIN      
        1 ttgattaaac gccgataaac aaatgattaa tttccatttc atcaattcat tatcatgata
       61 acgttcaaat gattgaataa ctgtttgata atttttagca gttgccaatt cttgatgata
      121 aaaaaccatc cagccaattg catcaattgc ttctgcaacc actttatctt caggataatt
      181 aatgatttct aaaatcgtag aaaaataatc aggattcatt ttagccatag ttctagcaat
      241 aatatcccgt ggcaaaggat agcttttttt ctttgatacc ttttcaggaa tcgtccgatg
      301 ttgattacct ctaattgtcc ccatataagg aatcattctt tttattgtca actcatttcc
      361 ggtagtcaaa atatcgcata tttctaattt agtatataag gctttttctt taaccaacat
      421 tctaagtaag atttcatcaa gttcattaat gtcaacaaag cttctaagaa taaataacgc
      481 cgcagtacga tttacggctg ttttatcttt taatagttct aataactcct cttttgaata
      541 taaaaagtag ggttcggctt ctgtatttgt taaaaagcca cgttttctaa gtgtttcagt
      601 atctgttttc atagtcttat tataccaaaa attaattcgc ttaaaaagtt tattactact
      661 tcaatctaca aataaaaagc cacagttatc aaactgtgac taaataactt cttcttccat
      721 tggtaaagtt actttaaatg ttgttccttc atgtaattta cttgttaatg taatatttcc
      781 ttgataatat tgaacgatat gtttgacaat cgctaatcct agaccagttc cccctgtttc
      841 tttatcacga ccttgatcac acctaaagaa acgttcaaat actcgtcctt gatcaatttt
      901 tgaaattcct cgtccagtat cactaacttc aatgataata ttacgaccaa attggtatga
      961 cttaattata attttaccac catcaacatt atacttaatt gcattagtaa tcaaattact
     1021 taataactga tgcatatgct gatgatttga tgtatatgta atatcttcta attctttatc
     1081 tacttgtaaa tgtttcttat caatctctac ttgcatcgta tcaattatct catctactag
     1141 tggtgttaaa tgaactggat gcttgattac atcaacatct ttattttcta atctcgatat
     1201 cattaaaata tcattaatta aagtagacat attattcact tcatcatgaa ttttatccaa
     1261 tgcttttttt gaagcatctt tatcattgat aactcccgtt tctaataatt cactatatcc
     1321 acgaatcgaa gtcattggtg ttttcaactc atgagaaaca ttagagaaaa attcctgacg
     1381 catcttcata gcattatgct gctcagtaat atttacaaac agcaaagtta ccccataatc
     1441 aaccttagcg acataacagt tatagaattc tttttccttt tcaacttcaa ctacctgttg
     1501 ttcatcactt aaatggtcaa gtgcattaat aattttaaag tcaaaaataa agtctttgat
     1561 tgaacaattt aacttgattg tatgaccata taatttttga gctttacgat taaccattaa
     1621 aactgttaaa tcactatcta ataagacaaa gccctctttc atttgatcaa gaatcccatt
     1681 gatttttatt tgttcacttt taagcttttt catagtatca tgaattgttt tagcctgttc
     1741 ttccaatttt gaagcaatga tattaaactc atcatattta taactatcaa attctagttc
     1801 acgataatcc ttcatttttg taacctgtgt cgtaatatcc tgaattggcg ctgtcaatgt
     1861 attagcaaat cgtttcgata agaataaagc aatgactaac gacattatcg cgcctactcc
     1921 tagcggtcta accaaagttg gaagattatc aaaaatacca ttatatggta aagccagacg
     1981 aactatatat cctttattaa atactgccac atagagcata tttcttttaa cagtacttga
     2041 gtaacgggtt gcataaccaa ccccttcact tagagcctgt ttaacttctt cacgaccttt
     2101 atgattttca tcaatttctt cactaccact atctgctaat acttcaccat tagtatcaat
     2161 gacagttaat ctagtttgat cattgtaagc aagtggattc aatgcatcta cttgtgcttt
     2221 taaatcgtgt gattcatcta attgataatc aaccaactta accgcgtaaa gcatatcatg
     2281 tttagtattt tccaacattt gacttgataa aataaccatt gaaacactgc ttgataaaac
     2341 tagagtgact aataaaactg tgagaaaata gcgaaaaata gttttattca ctaccatcac
     2401 cttcctcata aaaacgatat ccaacactac gtatcgtctt aatatactta tggccatcat
     2461 catttaattt acttcgtaaa gcacgaatat gaacatctaa agcacgagat tcaccaataa
     2521 aatcatatcc ccaaatttcg tttaataatt cttcacgttc aactacacga tgtttattat
     2581 ccattaaata gaccaataat tgatactctt tattagttaa ttctaatttc tgatcatcta
     2641 attttactaa acgtgtttgc ttgtcaacag taagatgttt agttctaata atatcgctag
     2701 taacatggcg tcttaataat gagcgaacgc gagcttgcaa ttcaagaatc ccaaacggtt
     2761 tagtcatata gtcatcagaa ccgctatcta aaccattaac cttatctatc tcacgatctt
     2821 tagcacttaa aattaagata ggtaattcac tatcagtttc acgaatttcc ttgattgcat
     2881 caattccgct catttttggt aacattaaat caaaaatagc taatgatggt gcttttttat
     2941 caatttctgc cagtgcatcg atagcattat caaattgaac aacttcataa ccattgctta
     3001 ctaatgctaa attaattatc tcgcggatat tttcatcatc ttcaactaca aatatacgtt
     3061 cacccatgtt cagtccccct tagatcaaac gataattatc aactgttcca gtttggttaa
     3121 attcaaccca ttcacaaata tctacagcat gatcaccaat tctttcgaaa tatttagcaa
     3181 tcattaggaa gttaatcgct aaatcgatcg tttcctttga ctcatttact atttgtatta
     3241 attctacctt aacttcttca aatagtccat ccatatcatc ttccaatttt tttacttctg
     3301 aagctttttt taaatcatgc tgatgaaaag cttcaattga ttgtttaacc atatttttag
     3361 caagatgagc catattaggt atatgttcaa ccattttata agggcaggtt acatctgtat
     3421 ctaataaaat ttctgcaata tctgctcctt ggtcaccaat tctttctaaa tctgtaacga
     3481 cttttaaagc tgtagacaca tttcttaaat cactagctac aggctgttgt tttaaaatta
     3541 acgatagaca acgagcttct atcgttcttt ccatatcatt aatcactttg tctccagcta
     3601 aaatatcacg gcataattca cgatctcctg atttaaatgc cacaatacag ttttcaattg
     3661 ctaaaataac tagatgacac atcttatcaa gatctacgtg taatttgttt aaatcatttt
     3721 caaaatttgt tcttaacatt tttttctcct atccaaatct tccggtaata taatcttcag
     3781 tacgtttatc tttaggcatt gcaaaaacat ccttagtttt tccatattca accatctcac
     3841 ctactaagaa aaacgcagta taatcagcga tacgactcgc ttgctgcata ttatgtgtaa
     3901 caattgcaat cgtatacttt tctttaagtt ccattaacaa ttcttcaatt tttaatgttg
     3961 aaattggatc caatgcactt gttggttcat ctaataagat cacatcaggt tctactgcta
     4021 aagcacgagc aatgcacaaa cgttgctgtt gtcctccaga taatcctaaa gcactgctgt
     4081 gcaaacgatc ttttacctct tcatataaag cagctgcttc taatgaatct ttaacaatct
     4141 tatctaactg ttttttattt ttgattccat gaattcttgg tccatatgcg atattatcat
     4201 aaatactcat cggaaacgga tttggctgtt ggaaaaccat tccaactttt ttcctcaaaa
     4261 ccgttgtatc aacacgacta tcataaatat cttcaccatc aagaaccaca ctaccagtta
     4321 tcttaatatc tttaacataa tcattcatgc gatttaatgt ctttaaaaat gttgatttac
     4381 cacaaccgga aggcccgata aaagccgtaa ttttatttgt tttaatatct agactaacat
     4441 tttttaaagc atgcttttca ccataataca gatctagatt ttttgcttca attttatttt
     4501 ccattctaat ccaccctatt tacatcaaat ttatttgtaa tcagttttgc taacatattt
     4561 aatcctaaaa caataacaat taaaactaag gcaatcacaa aggccgattc ataattagct
     4621 ttagccatct ccaaatataa ttgaatcgtt agtgttgcac ctgaacttga taaatgtgta
     4681 aagatatttt ttggcaaaac
//
LOCUS       DAOMTA010000143         4682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_487_length_4682_cov_2.427059, whole genome shotgun sequence.
ACCESSION   DAOMTA010000143 DAOMTA010000000
VERSION     DAOMTA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_4682_cov_2.427059"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..823
                     /locus_tag="PLK68_05215"
     CDS             44..823
                     /locus_tag="PLK68_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89846.1"
                     /translation="MIKAIFFDVDGTLISKSRSVLSEGVIAALKQLQEKGIKLFIASG
                     RHYLELDELGINAQFTFDGYLTLNGGYCFNQEGVIYKNPINSKDVERIVDHVTKHQLA
                     CSFVEGDDLYINLINDLVVAAQTAINTSLPPKKDVSRALINDVYQIDPFVTKEEIEEL
                     VALTQHCKYTQWHDGAYDIIPKQGGKQEGISAILDHYKIKVEETMAFGDGHNDIDMLQ
                     FVGTGICMENGCAETKAVCDYITDNVDNDGIVSALRYFGLL"
     gene            898..1419
                     /locus_tag="PLK68_05220"
     CDS             898..1419
                     /locus_tag="PLK68_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89847.1"
                     /translation="MKKIMKILVTTLCLCLVTACGARDKDVNLEKSGTKYSTDDGVSF
                     YYPSNYEITANATDTDTVEFSKDNNTLYFKVIKDETDNVVEDKDELYTGEIEQSGASE
                     IEVSKPVLDSGLDVYQYVFVHSDTGIRAKEIVYFSDDNTYIYGYRAAKDDFEDNDKDM
                     TVYLQSFSMATGK"
     gene            1678..3015
                     /gene="cysS"
                     /locus_tag="PLK68_05225"
     CDS             1678..3015
                     /gene="cysS"
                     /locus_tag="PLK68_05225"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRM89848.1"
                     /translation="MKIFNTLTNKKEEFKPLREGEVSIYVCGPTVYNYVHIGNTRPMI
                     VFDVLRRTFEYLGYKVTFVSNFTDVDDKIIKAAKAEGITEKELTDKYIAAYEDVRRNL
                     NLLFPTYAPRVTNTMDAIIKFIDNLVKSGYAYEVDGDVYFRVSKIDEYGQLSGIKIED
                     LVAGASERIDENDKKEESTDFALWKKTDEGIRFDSPWSKGRPGWHTECVVMINDIFEG
                     GKIDIHGGGQDLKFPHHENEIAQSMACHHHPIAHTWMHNQMINIDNQKMSKSLGNVIW
                     AKDMVAELGCNVVKWFMLSSHYRNPLNLTEEVLNSVKKEVAKVDNVIKSVSLYLQVNH
                     IANENYNKAAVDGMVGALEDDLNTSLALTQVLDQVKVLNQVMRVKEKNDKAIAIEYQT
                     LLKMTAVIGFVFEPRKLNAAELEIYQAWLEAKQNKDFETADKLRTQLIEKGII"
     gene            3015..3392
                     /locus_tag="PLK68_05230"
     CDS             3015..3392
                     /locus_tag="PLK68_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRM89849.1"
                     /translation="MRPELINASVLAYLGDSIFEVLVRDYLVKESGFVKPNDLQREAV
                     KYVSASSHAAFMHDMLDEEFFSADEVGTYKRGRNTKGSKNESLDHMHSTGFEAVIGTL
                     YLEENFDRIKVIFERYKQYINNK"
     gene            3430..4155
                     /gene="rlmB"
                     /locus_tag="PLK68_05235"
     CDS             3430..4155
                     /gene="rlmB"
                     /locus_tag="PLK68_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRM89850.1"
                     /translation="MKQYIYGKNTILEALKGDKSVYTVYVQNNAKDNGIIEQCKRKKI
                     PFKIVDKSEFIKKLGNVSHQGVMAEIEEYRYYSIDEIINTIPDGKQPLLLMLDGLEDP
                     HNLGAILRTCDAIGVDGVIIGKNRSVGLNGTVAKVSTGAIDHVKVAQVTNLTRTLEDL
                     KKRSFWVVGCDLDKSQDYRQVDYNMPLVIVIGSEGFGISRLVKKSCDINVVLPMVGHV
                     NSLNASVATAVILYQVYNSRNPL"
     gene            4172..>4682
                     /locus_tag="PLK68_05240"
     CDS             4172..>4682
                     /locus_tag="PLK68_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRM89851.1"
                     /translation="MNGYEIEELYYLYRQGCPIAQALLIEYCYWQIKMMMPAYCYTMT
                     SYQSDYKDYLQVVIIRCLLALDNYRPDRGMQVRSFISMVIQNTISSLIVKNQGKVVRE
                     RQTLFSLDDYCSDDERIRYVDAIADNKSPDLLLIDQEQQKRVDAYILNVCSAWEQTVI
                     AYHKCGFKDQ"
BASE COUNT         1619 a          548 c         1029 g         1486 t
ORIGIN      
        1 aaaagacaga gcaatctgtc ttttcctttc caaaggagaa ggtatgataa aagcaatttt
       61 ttttgatgta gatggaacat tgatttctaa aagcagatca gtgcttagtg agggtgtaat
      121 agcagcttta aaacagcttc aagaaaaggg aatcaaatta tttattgcaa gtggacgtca
      181 ctatttagaa ttagatgagt tagggataaa tgctcaattt acatttgatg gttacctaac
      241 attgaacgga gggtattgtt ttaatcagga aggggttatt tataaaaatc ccattaattc
      301 taaagatgta gaaagaatcg ttgatcatgt aactaaacat cagttggctt gtagttttgt
      361 tgaaggagat gatttgtata ttaatttaat taatgatctg gttgttgctg cacagacggc
      421 aatcaatacc tcattaccac cgaaaaaaga tgtttcacga gccttgatta atgatgttta
      481 tcaaatcgat ccttttgtaa caaaagagga gatagaagaa ctggtagctt taacgcaaca
      541 ttgcaaatat actcagtggc atgatggagc ttatgatatt attcccaagc aaggtgggaa
      601 gcaagaagga atcagtgcta tcttggatca ttataaaatc aaggttgagg aaacgatggc
      661 ttttggtgat ggacataatg atatagatat gctccagttt gttggtacgg gaatttgtat
      721 ggaaaatggc tgtgcagaaa caaaggcagt gtgtgattac ataacagata atgtcgacaa
      781 tgatgggatt gtgtcggctt tacgatattt tggcttatta taaaataatt gtgggctaaa
      841 taaggttgta atcggttgga gagttgtgta aaatgggata gtagaggagt ggttgtaatg
      901 aaaaaaataa tgaaaatact tgttacaact ttatgcttgt gtcttgtgac agcttgtggc
      961 gcgcgggata aagatgttaa cttagaaaaa agtggaacta agtatagtac tgatgatgga
     1021 gttagttttt attatccaag taattatgag attactgcaa atgcaactga tacagataca
     1081 gtagaattct ctaaggacaa caacacattg tattttaaag taattaaaga tgaaactgat
     1141 aatgttgttg aagataaaga tgaattatat actggtgaaa ttgagcaaag tggtgcttcg
     1201 gagattgagg tatccaaacc ggttttagac agtggtcttg atgtttatca atatgtattt
     1261 gttcatagtg atactggaat tagagccaaa gagatagttt actttagtga tgataatact
     1321 tatatttatg gatatcgtgc tgctaaagat gattttgagg ataacgataa agatatgaca
     1381 gtatatttgc agagtttttc tatggcaaca ggtaaataag ttgtatttca tgaatgattt
     1441 tgttaagata aacatatatt taaattatag gtaaacgaag tagattatta gaagttaata
     1501 gagatagcct gatggtggaa acggctaaat ggaaataatt gaactgccct tatctgagtc
     1561 ttttgaaaag aaagaacgtg aactagcgtt aatagtaatc ttaaagcatc tattatcggg
     1621 tggaagaaca caatggtgtc ctgatagtaa tatgcttttt tatttatgga ggtaatgatg
     1681 aaaatattta acacccttac aaataagaag gaagaattta aaccgcttcg cgaaggtgaa
     1741 gtaagtattt atgtatgtgg gccaacggtt tataattatg ttcacattgg taatactcgc
     1801 ccgatgatcg tctttgatgt acttagaaga acatttgagt atttaggata taaagtgact
     1861 tttgtatcaa actttactga tgttgatgat aaaatcatta aggcggctaa ggcagaagga
     1921 ataactgaaa aagaacttac tgataaatat attgctgctt atgaggatgt tcgtcggaat
     1981 ttgaatttat tattcccaac ttatgcacct agagttacga atacgatgga tgcaatcata
     2041 aaatttattg ataatttagt taagagtggt tatgcttatg aggttgacgg ggatgtttat
     2101 tttagagtat ctaaaattga tgagtatgga caattatcag ggatcaaaat cgaagattta
     2161 gtagctggag cgagtgaacg aatcgatgaa aatgataaaa aagaagaatc aactgatttt
     2221 gcattatgga aaaagacaga tgaaggtatt cgctttgata gtccatggtc aaaaggacgt
     2281 cctggctggc atactgaatg tgtagttatg atcaacgata tttttgaagg tggtaagatt
     2341 gatattcacg gtgggggaca ggatttgaaa ttcccacatc atgaaaatga aattgctcaa
     2401 tcaatggctt gtcatcatca tccgattgca catacttgga tgcataacca aatgatcaat
     2461 attgataatc aaaaaatgtc taaatcacta ggaaatgtta tttgggcaaa agatatggtt
     2521 gctgaattgg gctgtaatgt agttaaatgg tttatgttat caagtcatta tcgtaatcca
     2581 ttaaatttaa cggaagaagt tttgaatagc gtgaaaaaag aagttgctaa agttgataat
     2641 gtaatcaaat cagtttcttt gtatttacaa gttaatcata tcgctaatga aaattataat
     2701 aaagcggcag ttgatgggat ggttggagct ttagaagatg atttaaatac atctctcgca
     2761 ttaacacaag tattagatca ggtaaaagta ttgaatcagg tgatgcgtgt gaaagaaaag
     2821 aatgataaag ctatagcaat tgaatatcaa acactattaa aaatgactgc agtgattggc
     2881 tttgtttttg aaccgcgtaa attaaatgca gcggaactag agatatatca agcatggctt
     2941 gaagctaaac aaaataagga ttttgaaact gctgacaaat taagaactca attaattgaa
     3001 aaggggatca tttaatgcgc ccggaattaa tcaatgcaag tgtattagct tatctgggtg
     3061 acagtatttt tgaggtgcta gtacgtgatt atttagttaa agaaagtggt tttgttaaac
     3121 cgaacgatct tcagcgtgaa gcagtaaaat atgtatcggc aagctcacat gctgcattta
     3181 tgcatgatat gctggatgaa gaatttttta gtgctgatga agttggaact tataaacggg
     3241 gacgcaatac caaagggagt aaaaatgaaa gtcttgatca tatgcattca acaggatttg
     3301 aagcagtcat tgggacttta tacttagaag aaaactttga tcgaattaaa gtgatctttg
     3361 agcggtataa acaatatatt aataataaat aaaatcgaga taaatttgag acgaaattag
     3421 gtgaaaaata tgaaacaata tatatatggt aaaaacacaa tattagaagc tttaaaggga
     3481 gataagagtg tatatactgt gtatgtccaa aacaatgcta aagataatgg cataattgag
     3541 caatgtaaaa gaaaaaaaat tccattcaag attgttgata agtctgaatt tatcaagaaa
     3601 cttggaaatg ttagtcatca gggagtgatg gcggaaattg aagaatatcg ctattatagt
     3661 attgatgaga tcatcaatac tattcctgat ggtaaacagc cactattatt aatgcttgac
     3721 gggttagaag atccacataa tctaggagca attttaagaa cttgtgatgc tattggggtt
     3781 gatggtgtta tcattgggaa aaatcgtagt gttggtttaa atgggacggt tgcaaaggtt
     3841 tcaacgggtg ctattgatca tgttaaggtt gctcaagtta caaatttgac tagaacatta
     3901 gaggatttaa aaaagcgttc tttttgggtt gttggctgtg atttagataa atcccaagat
     3961 tatcgtcagg ttgattataa tatgcctttg gtaatagtaa tcggttcaga aggttttgga
     4021 atatcaagac tagttaaaaa aagttgtgat atcaatgttg tcttgcccat ggtagggcat
     4081 gttaattcgt taaatgcttc tgtggcaaca gcagtgattt tatatcaggt atataattct
     4141 cgtaatcctt tatagtagga aggaggtaac catgaatggt tacgaaatcg aagaattgta
     4201 ttatttatat cgtcaggggt gtccgattgc acaggcactt ttgattgaat attgctactg
     4261 gcaaattaag atgatgatgc cggcgtattg ttatacgatg acctcatatc agagtgatta
     4321 taaagattat cttcaagtag taattattcg ttgtttattg gctttggata attatcgtcc
     4381 tgatcgtggc atgcaggtta gaagttttat ttcaatggtg atccaaaata cgattagttc
     4441 tttaattgtc aaaaaccaag gtaaagtagt tagggaacgg caaactttat tttctttgga
     4501 tgattattgt agtgatgatg agcgaatcag atatgtagat gcaatcgctg ataataagag
     4561 tccagatcta ctattaattg atcaagaaca acaaaagagg gttgatgcgt atattttaaa
     4621 tgtttgctcg gcttgggaac aaacggtaat tgcttatcat aagtgtggtt ttaaagacca
     4681 gg
//
LOCUS       DAOMTA010000144         4680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_489_length_4680_cov_2.128649, whole genome shotgun sequence.
ACCESSION   DAOMTA010000144 DAOMTA010000000
VERSION     DAOMTA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_4680_cov_2.128649"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(489..1871)
                     /locus_tag="PLK68_05245"
     CDS             complement(489..1871)
                     /locus_tag="PLK68_05245"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM89852.1"
                     /translation="MEKKPVKIVTIGGGSSYTPELMEGFIKRYDELPIKEIWLVDIEA
                     GKEKLEIVGAMAQRMWDASPYDVKVHLTLDRREALKDADFVTTQFRVGLLNARIKDER
                     IPFSYGMLGQETNGAGGIFKAFRTIPVILSIVEDMKELCPNAWLVNFTNPSGMVTEAV
                     MKYGKWDRVIGLCNVPVGAMMAEPETIGTTLDKLIYKFAGLNHFHWHRVFDLDGTDVT
                     GKIIDAMYEGKDSGIPANIHDIPFFKEQLKQMNLIPCGYHRYYYRQQEMLAHGLEEYN
                     GIGTRGQQVKQTEAELFELYKDPNLNYKPEQLTKRGGTHYSDAACETIASIYANKNTH
                     LVVTTKNNGAVPDLPADCAVEVSAHIGSAGALAIAFGPLEPAQRGWLQCMKNMELCVE
                     EAAVTGDYGLALQAFILNPQIPSGENAKRVLDELLIAHKKHLPQFADKIAELEAAGVT
                     VKDEIARDLD"
     gene            complement(2047..3156)
                     /locus_tag="PLK68_05250"
     CDS             complement(2047..3156)
                     /locus_tag="PLK68_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM89853.1"
                     /translation="MPRKQTFKRRPNKAGTVIKLSGKRRRPWCAKITTGKDIITGRQI
                     QTVLGTFETWDEADDALTLYRLGQKNKITDTEAEALAPDTFQKLVDQREKNMPTFKEI
                     FDIIYQEELCILSKSAAQGYRSWIKHFNSIYHHKISQISLADLQEIFDRDKAGYGTKV
                     HMKVLVSKIFEYAVIHKYINRDDDYTEYIKCGKAKESTKHYAFSNDEIRALMSDNSDT
                     AKIILIYIFTGLRANELLNIPRKNICLNTEFPYLVSGSKTDAGKNRVIPIHTFIEPFV
                     KQLLMKKKKRIIDCTYYQFSSMFSSFLTNHNMKHTIHDTRDTFATLCQSNNVDLFIRK
                     RILGHKMKDITFDTYTSTVIETLYKEINKIKVPKL"
     gene            complement(3307..3636)
                     /locus_tag="PLK68_05255"
     CDS             complement(3307..3636)
                     /locus_tag="PLK68_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89854.1"
                     /translation="MKKVLSLLLVLGLCFGLAGCGGSSKPDNMTDESYNAAKEVLEIA
                     DKYLDDKIDKDEAADSIRSTMDGFEGDGVGDIKVRERAKQITSSLYISESSVQEARDN
                     LAEVLEN"
     gene            complement(3718..>4680)
                     /locus_tag="PLK68_05260"
     CDS             complement(3718..>4680)
                     /locus_tag="PLK68_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HRM89855.1"
                     /translation="FILPFFQALGYDVFNPLEFIPEFTADVGIKKHEKVDYAILQEGK
                     PLILIEAKSCNEKLDKHDSQLFRYFGTTESKFAILTNGIIYKFYSDLDQPNVMDSQPF
                     YVLDMMDLSDQAIQYLANFNKCNLDIDSIMNTASDLKYLSLTKTAFKELIENPTDEFI
                     KLLLNSGVYDGLKNQKVIDKFKPIVKRGINQYINDKMSSKFKETLSSNDDEVIEENNE
                     PDEEVSKINTTIDELNGFAIVKAILRTEVEAKRITYKDTESYFGILLDNNIRKWICRI
                     YINTKAKYVIISDENKKGIKHDLGTLDDLYNLSNELKDSLKKYL"
BASE COUNT         1428 a          809 c          662 g         1781 t
ORIGIN      
        1 tctattgtct tttagacctt cttcacctaa ttctaaatat ttctttaccc attgatatac
       61 ttgtgagtaa gaaacctcaa aaagttcagc tgctgattta tattctttct cataatctaa
      121 ataatattat acaattttaa ttatttcttc taatgttgct ttttttgatt tggtcatgta
      181 gacatctcct ttaggatcat aatctgttaa tttttagtga ctattataac tgatagccca
      241 acttcataaa gatgaagaag aatgagtatt gtatttattg cttaaatctc tcaaagaatc
      301 tttactattt aaatgctctt gaattattat ttgttttatt tctttaattc tgttcttata
      361 ctctaaaacc gcttgtatct ttttttattg tatattacgt tccataagaa aagatccctc
      421 ttttcatgga caactttatt taagttgtct attttagaga gatcatatca aattcacgaa
      481 ttttttattt agtctaaatc tctagcgatt tcatctttta ctgtaacacc agctgcttct
      541 aattcagcga tcttatcagc aaattgtggt aagtgttttt tatgagcaat taataattca
      601 tctaaaacac gtttagcatt ttcaccagaa ggaatttgtg gatttaagat aaatgcttgt
      661 aaagctaatc cataatcacc agttacagca gcttcttcaa cacataattc catatttttc
      721 atgcattgta accaaccacg ttgagcaggt tctaatggtc caaaagcgat agctaaagct
      781 ccagctgaac cgatatgagc agatacttct acagcacaat cagcaggaag atcaggtaca
      841 gcaccattgt ttttagtagt tacaacaagg tgagtatttt tattagcata gattgatgcg
      901 atagtttcac atgcagcatc tgaatagtga gtaccaccac gtttagttaa ttgttcaggt
      961 ttgtaattta aatttggatc tttatataat tcaaataatt ctgcttcagt ttgttttact
     1021 tgttgtccac gagttccaat tccattatat tcttcaagcc catgagctaa catttcttgt
     1081 tgacgatagt aatatctatg atatccacaa ggaattaaat tcatttgttt taattgttct
     1141 ttaaagaatg ggatatcatg aatatttgca ggaataccag aatctttacc ttcatacatt
     1201 gcatcaatga tttttccagt tacatctgta ccgtctaaat caaaaacgcg gtgccagtgg
     1261 aaatggttta atccagcaaa tttataaatt aatttatcta aagttgttcc gattgtttct
     1321 ggttcagcca tcatagcccc aactggtacg ttacataaac caattacacg atcccattta
     1381 ccatatttca taactgcctc agtaaccata ccactaggat ttgtgaagtt tactaaccat
     1441 gcatttggac ataattcttt catgtcttca acaatgctaa ggattacagg aattgttcta
     1501 aatgccttga agattcctcc agctccattt gtttcttgtc ctaacatacc ataagagaat
     1561 ggaattcttt catctttaat acgagcgttt aataaaccaa ctctgaattg agtagtaacg
     1621 aaatcagcat cttttaaagc ttcacgacga tctaaagtta aatgtacttt aacatcgtaa
     1681 ggagaagcgt cccacatacg ttgtgccata gcaccaacga tttctagttt ttctttacca
     1741 gcttcaatat ctactaacca aatttctttg attggaagtt catcatatct tttaataaat
     1801 ccttccatta attcaggagt ataactacta cctccaccaa ttgttacaat ttttacaggt
     1861 ttcttttcca tcttatttcc tccgtatatt tattttacac ataaagcata cacgtttatt
     1921 caaataaatg ttttgttttt tcaagacttg aaaatatcta tccatcttat tgacgataaa
     1981 tcaagggttt caaacgatta gaagcagtta aatgtacctc gtaagtaaca aataagtaac
     2041 aaaaaatcaa agcttaggca ccttgatttt gttgatttct ttatataatg tttcaattac
     2101 ggtagatgta taagtatcaa aggttatatc tttcattttg tgaccaagaa tacgttttct
     2161 tataaacaga tcaacgttgt tagactgaca taatgttgca aatgtatccc tagtatcatg
     2221 tattgtatgc ttcatattat gattagttaa aaaagaggag aacatagatg aaaactgata
     2281 gtaggtacag tcaattattc ttttcttttt cttcatcaac agctgtttaa caaaaggttc
     2341 gataaaagta tgaatgggaa ttactcgatt tttacctgca tcagtttttg atccagatac
     2401 taaatagggg aattctgtgt ttaaacaaat atttttacgc gggatattca gcagttcatt
     2461 tgctcttaaa ccagtgaata tataaataag tattatttta gccgtatcgc tgttatcgct
     2521 catcaatgct cttatttcgt catttgagaa agcataatgt ttagtgcttt cttttgcttt
     2581 gccacatttt atatattcag tgtaatcatc gtcacgattt atatatttgt gaataactgc
     2641 atattcaaat attttgctaa ctaaaacttt catatgtact tttgtaccgt aaccagcttt
     2701 atcacgatca aatatttcct gcagatcggc caaacttatt tgactgatct tgtggtggta
     2761 tatactatta aaatgtttaa tccacgatct ataaccttga gcagcacttt ttgataatat
     2821 gcataattct tcctgatata taatatcaaa gatttcttta aaggtaggca tattcttttc
     2881 acgctgatca acaagcttct ggaatgtatc gggtgcaagt gcttcagctt ccgtatcagt
     2941 aatcttattt ttttgaccaa gcctgtataa agtcaaagca tcatcagcct catcccaggt
     3001 ctcaaaggtt cccagtactg tttgtatctg tctaccggta ataatatctt ttccagtagt
     3061 tattttagcg caccagggac gtcttcgttt acctgatagt tttattactg ttcctgcttt
     3121 atttggcctg cgcttaaaag tctgctttct aggcataatg aaaacacgtc ctttctataa
     3181 tttgccttag acgtgtttaa atgatataat taagcacgta aaaggacttt tggttagtta
     3241 ctttacatta gtgatattgg cgtatcactt tgaccatccc attgcagtgg ggtggtttta
     3301 tttattttaa ttttctagaa cttctgctaa attgtctctt gcttcttgaa cagaggattc
     3361 gctaatataa agtgaagatg ttatttgttt tgctctttct ctaactttga tatccccaac
     3421 tccatctcct tcaaacccat ccatagtaga gcgaatagaa tcggctgctt catctttatc
     3481 gattttatca tcaagatatt tatcagcgat ttctaaaacc tctttagcag cattgtatga
     3541 ttcatcagtc atattatctg gtttagaact tcctccacat cctgctaatc caaagcaaag
     3601 ccctagaact aataataaac ttaatacttt cttcataatt taattctccc ttcaattttt
     3661 tattttatat aaacactatg aagtgtgttt gccgtatttg gttatgtata gatttaatta
     3721 gagatatttt tttaaagagt ctttcaattc attagataaa ttgtatagat cgtcaagggt
     3781 acctaaatca tgttttattc cttttttatt ttcatcagaa ataattacgt atttagcttt
     3841 agtgttaatg taaatacggc aaatccattt acgtatatta ttgtctaata aaataccaaa
     3901 gtaactttct gtatctttat atgtaatacg cttagcttct acttcagtac gtaaaatggc
     3961 tttaacaatt gcaaaaccat ttaactcgtc aatagtggtg ttgattttgc ttacttcttc
     4021 gtctggttca ttattttcct caatgacttc atcatcatta ctgcttaatg tctccttgaa
     4081 ttttgaagac attttgtcat taatatattg attaattcca cgttttacaa taggtttaaa
     4141 tttatcaata actttttgat ttttcaatcc atcatacacg ccgctgttta ataaaagttt
     4201 tataaattca tcagttggat tttcgataag ttctttaaat gctgtttttg ttaaactgag
     4261 atattttaaa tcagatgcag tattcattat actgtcaata tccagattac atttattaaa
     4321 gttagctaaa tattgaattg cttgatcgct taaatccatc atgtccaaaa catagaatgg
     4381 ttgtgagtcc ataacatttg gctgatctaa atcactataa aatttgtata taattccgtt
     4441 tgttagaatt gcaaatttag attcagttgt accaaaatat ctaaataatt gtgaatcatg
     4501 tttgtctaat ttttcgttgc atgattttgc ttcgattaag attaatggct taccttcttg
     4561 caatatagca tagtcgactt tttcatgttt tttaattcca acatcagctg taaattcagg
     4621 tatgaattca agcggattga aaacgtcata accgagagcc tgaaaaaatg gtaaaataaa
//
LOCUS       DAOMTA010000145         4655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_492_length_4655_cov_2.572391, whole genome shotgun sequence.
ACCESSION   DAOMTA010000145 DAOMTA010000000
VERSION     DAOMTA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_492_length_4655_cov_2.572391"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PLK68_05265"
     CDS             complement(<1..1531)
                     /locus_tag="PLK68_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRM89856.1"
                     /translation="MAISRLNLVSINFDKYRYNEVLLKLFSHDDFHPELASKFIDSVT
                     GLSVYNQDNLYEEILVRLNDASNKYHFKLEEVPVETNQINVLRVKEYLDDLLLDVERI
                     DAVKVQLDRMIIENQEAIIQLQHVADIDVDFDDLFSCKYLQVRFGKIPVNNVSKLDYY
                     ESLPFVFKAFHNDNQYTWCMYITTPGDAPEVDNIFSSLYFERIHIPAFVHGSPELAIG
                     EIQEEADVAKEQSEKLKERIDKMFANDRDKLNEIYTIAKHLNDVFIMQKYVVVIGDKL
                     SVNGFVPTRSVKKFKEDFETLEKVTVDIKPANSDVRLSPPTLLKNNWFSRPFRMFVEM
                     YGVPKYNDADPTLLVALSYTLLFGIMFGDVGQGVILSLVGFVAYKKFGLQLGAVGVRL
                     GISSMLFGVVFGSVFGNEEILIPLFNAMSPDNTMTLLIAAICLGIILIIISMAFNVIL
                     SFKKKNFGEAVLSQNGICGLVFYISILGLVINMFLGLGFVNVIYVVGLIALPLFLIFL
                     KE"
     gene            complement(1544..2905)
                     /locus_tag="PLK68_05270"
     CDS             complement(1544..2905)
                     /locus_tag="PLK68_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89857.1"
                     /translation="MAIIKTHFFNISFEHKDLMKMLIKMTEYQDEMFPQDSKKIANNV
                     KGVSVMDEANPYNEPLDNIYHILNRLNLESNVQDNEFKEINLHNANKLIDDINDKIDN
                     IVKIKEDITKEKEENDEAIILLKNLEESKISVDDVKNTKYITCRFGKIPVNEFTKIQY
                     YRDYEFIFRELNRSKQYVWIVYAGLTSSISEIDNAFSSMSFEPISLPEFAHGKVHEAI
                     DELTEESTAMEQYIAKMDSKLEDIKKEYKEEVLETFTRLYNLKRLFDKCRYVVDFSQK
                     ASIYTFSSFDLKEAEAKFDDIDSVKVMELPVNIYENRNIVAPVLVRNNSLMQPFENIL
                     SVTLGDTFDPTTIVALLTMLIGAVCVGDIGVGALLIVLGFLFTIKKPNNFGGMLKRLG
                     AAVLIGGLFYGTAFYRIELYQPLASLPLHVIHTFLFGFSLWIIAMVILIIVKKVTRKS
                     IKI"
     gene            complement(2919..3950)
                     /locus_tag="PLK68_05275"
     CDS             complement(2919..3950)
                     /locus_tag="PLK68_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRM89858.1"
                     /translation="MALSSNALCAKAKAMYGYRIGEEGYSDLCRKQSLSEMVTYLKSQ
                     TKYSGVLEDINVRNVHRRQVEAALNKEYFERCARLMKYAPKKNQDFYSQEVIGIEVQL
                     IVDKVVSIKEKDQASFSLEIPDYLASKMSFNIYGLINVDNYKDLVTYLRDTRYYKILA
                     DFDFTAPIDINALERQMKSLFYSTYVAAIKKNFKGKEQKELLDILSTSIELINITKIY
                     RFKKYFKESNETIKNSLYLEHCRMSSSMLDTLIEARSAKELMELLANSKYKLYLGDKD
                     YAYIEYYVEEVRYNIAKRYMRFSSNAPLVYLTYSILQKVEVDNLKHIIEGIRYGRDAS
                     SIEEMLIFA"
     gene            complement(3963..4271)
                     /locus_tag="PLK68_05280"
     CDS             complement(3963..4271)
                     /locus_tag="PLK68_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89859.1"
                     /translation="MDDVIQRLVEIDRKCVERVEAAKAKKLDAQTNMNEKRKEIYDGF
                     VTEQNKKIEAHKAELMNKNKEEAKQLDQDYNDTVANLENLYNQNKDKWINQIVERCLK
                     "
BASE COUNT         1585 a          886 c          542 g         1642 t
ORIGIN      
        1 gttcctttag gaaaattaga aatagtggta aggcaatcaa tccaactaca taaataacat
       61 taacaaatcc taatcctaaa aacatattta taactaatcc caaaatagaa atataaaaga
      121 ctaatccaca aattccattt tgacttagca ctgcttctcc aaaattcttc tttttaaaac
      181 tcaaaataac attaaacgcc atcgaaataa taattaaaat tattcctaaa caaatcgcag
      241 cgatcaataa tgtcatcgtg ttgtctggcg acattgcatt gaataacgga atcaaaattt
      301 cctcatttcc aaataccgaa ccaaagacaa ccccaaaaag catactggaa attcctaaac
      361 ggactccaac cgcacctaat tgtaaaccaa actttttata agccacaaat ccaacaagtg
      421 ataaaataac cccttgtcct acgtcaccaa acataattcc gaataacaag gtatatgata
      481 atgccactaa taaagttgga tcggcatcat tatatttagg taccccatac atttcgacga
      541 acattctaaa cggccgtgaa aaccaattat ttttaagcaa tgttggtggc gataaacgaa
      601 catcactatt agctggttta atatcaactg ttactttctc taatgtttca aaatcttctt
      661 tgaatttttt aacactacgt gttggaacaa aaccattgac agaaagttta tcaccgatta
      721 caacaacata tttttgcatg ataaagacat catttaaatg ttttgcaatc gtatagattt
      781 cattcaactt atcacggtca ttagcaaaca ttttatcaat tcgctctttt aatttttcac
      841 tttgttcttt agcaacatca gcttcttctt gaatctcacc aatagcaagt tctggtgaac
      901 cgtgaacaaa ggccggaata tgaatccgtt caaaatacaa tgatgagaaa atattatcaa
      961 cttctggtgc atcaccaggc gttgtaatat acatacacca agtatattga ttatcattgt
     1021 gaaatgcttt aaagacaaat ggtaatgatt cataataatc taatttagaa acattattta
     1081 ctggaatttt tccaaatcta acctgaagat atttacagct aaacaaatca tcaaaatcaa
     1141 catcaatatc agcaacatgt tgtaactgaa tgattgcttc ctgattttca ataatcatcc
     1201 gatctaattg tacttttaca gcatcaattc gctcaacatc aagcagtaga tcatctaaat
     1261 attcttttac ccgcagcaca ttgatttgat tagtttcaac tggcacttct tctagcttaa
     1321 aatgatactt atttgatgca tcattcaatc gcaccaagat ttcttcataa agattatctt
     1381 gattgtaaac acttagtccc gttacactat caataaactt agaggccaac tcaggatgaa
     1441 agtcatcatg gctaaaaagc tttaataaaa cttcattgta acgatactta tcaaaattaa
     1501 tactaactaa gtttaatctt gaaatcgcca ttatatccct cctctatatc ttgattgatt
     1561 ttcgcgtaac ttttttcaca ataattaaaa tcaccatcgc aatgatccaa agactgaatc
     1621 caaataagaa agtatgaata acatgtaatg gtaaacttgc taacggctgg tacaattcaa
     1681 ttcgataaaa cgccgttcca taaaataaac caccaattaa aacagcagct cctaaacgtt
     1741 ttaacatacc accaaaatta ttaggttttt taattgtaaa taagaaccct aatacaatta
     1801 ataaagctcc aacaccaata tcaccaacac aaacagcacc gataagcatc gttaataaag
     1861 caactatcgt cgttggatca aaagtgtcac caagtgttac actcaagata ttttcaaacg
     1921 gttgcattaa actattattt cttactaata ctggtgccac aatatttcta ttttcataga
     1981 tattaactgg tagttccata acttttacac tatcaatatc atcaaatttt gcttcagctt
     2041 cttttaaatc aaaactactg aaagtgtaga tcgaagcttt ttgtgaaaag tccacaacat
     2101 aacgacattt atcaaatagt ctttttaagt tatataatct tgtaaatgtt tctaaaacct
     2161 cttctttata ttcctttttt atatcctcta gtttagaatc cattttagca atatattgtt
     2221 ccatagctgt tgactcttca gttaattcgt caattgcttc atgaacttta ccatgagcaa
     2281 actccggtaa gctaattggc tcaaatgaca ttgatgaaaa tgcattatca atttcagaaa
     2341 tactgcttgt aagtccagca taaacgatcc aaacatattg tttagaacga ttcaattccc
     2401 taaaaataaa ttcataatca cgataatatt gaatttttgt aaattcattc acaggaatct
     2461 ttccaaatcg acaggtaata tatttagtat tcttaacatc atcaacactt atttttgatt
     2521 cttctagatt tttcaacaaa atgattgctt catcattttc ttctttttct tttgtaatat
     2581 cttctttaat tttgacaata ttatcaattt tatcattaat atcatctatt aatttatttg
     2641 cattatgcaa gttgatttct ttaaattcat tatcttgaac attactttca agatttaaac
     2701 gatttaagat gtgataaatg ttatctagtg gttcattata cggattagct tcatccataa
     2761 ctgatacccc tttaacatta tttgctattt ttttagaatc ttgaggaaac atttcatctt
     2821 gatactcggt catcttaatt agcattttca tcaaatcttt atgttcaaag gaaatattaa
     2881 agaaatgtgt ttttataata gccattcatt aatccctcct atgcaaagat cagcatctct
     2941 tcaatactag atgcatctcg tccatatcta attccctcaa taatatgttt tagattatca
     3001 acctctacct tttgtaagat tgaatatgtt aaatatacta acggtgcgtt actagaaaaa
     3061 cgcatatatc ttttagcgat attatatcta acttcttcaa cataatattc gatataagca
     3121 tagtctttat cacctaaata cagcttatat ttagaattag ctaataattc catcaattct
     3181 tttgcactac gagcttcaat taatgtatcc aacattgatg aagacattcg acaatgctct
     3241 aaatataaag aatttttaat tgtttcattc gattctttaa aatatttttt gaatcgatag
     3301 atcttcgtaa tattgatcaa ctcaatcgat gtcgataaaa tatctaataa ttctttttgt
     3361 tctttaccct taaaattctt tttaatggca gccacataag tactataaaa tagtgatttc
     3421 atctgacgtt ctaaagcatt aatatcaatc ggtgctgtaa aatcaaaatc agctaatatt
     3481 ttataatacc tagtatctct taaatatgtt actagatcct tatagttatc aacattgatc
     3541 aatccataaa tattgaaact cattttgctt gctaaataat caggtatctc caaagaaaaa
     3601 ctcgcttgat ctttttcttt aatactgaca accttatcaa caatcaattg aacctcaatt
     3661 ccaatgacct cttggctata gaaatcttga ttcttttttg gtgcatactt cattaatctc
     3721 gcacaacgtt caaagtattc tttatttaaa gcagcctcta cttggcgacg gtgaacgtta
     3781 cggacattaa tatcttctaa aacaccacta tactttgttt gcgattttaa gtatgttacc
     3841 atctcactaa gtgactgttt ccggcataga tcactataac cttcctcacc aatgcggtat
     3901 ccatacatcg ctttagcctt agcacatagt gcatttgacg ataaagccat accttcacct
     3961 tcttacttta aacatctttc gacaatctga ttgatccatt tgtccttatt ttgattataa
     4021 agattctcta aattagctac agtatcatta taatcttgat caagctgctt tgcttcttct
     4081 ttgtttttat tcattaattc agctttatgc gcctcaatct ttttgttttg ttcggtgaca
     4141 aaaccatcat atatctcttt tcttttttca ttcatgttgg tttgcgcatc taatttctta
     4201 gcttttgcag cttcaacacg ttcgacacat ttacggtcta tttctactaa cctttgaatt
     4261 acatcatcca tagtataagc cccctttgcc tgagataagt atactcctat attttaaaaa
     4321 tgcaacgata ataatctatc aattgaaaaa aatagtcatt ttcgctgtat ccctagtctg
     4381 acaagggttt acaacgattt aacaaacttt ttaaagactt atattttttt cgtgaaaaat
     4441 aattatcatg cttttttaac atgataatta tagcgctaag tttgataaaa tcactagaca
     4501 atctatgcga tttgtctagt aaacaagatt ttgaaaacgc ttcgaataaa aggtcctgca
     4561 attaacagtt gtagtggtaa agctgcaata aaattttttg gaatatttgt caaccacacc
     4621 attaaagtct gttcactgat tccagcctgt tcaac
//
LOCUS       DAOMTA010000146         4646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_495_length_4646_cov_2.865171, whole genome shotgun sequence.
ACCESSION   DAOMTA010000146 DAOMTA010000000
VERSION     DAOMTA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_495_length_4646_cov_2.865171"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="ssb"
                     /locus_tag="PLK68_05285"
     CDS             complement(<1..479)
                     /gene="ssb"
                     /locus_tag="PLK68_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM89860.1"
                     /translation="MINRVVLVGRMTRDPELRRTPQGDAVTSFTLAVNRNFTSRDGQQ
                     QADFINCVVWRKPAENVERYCSKGSLVGVEGRIQTRSYDNTQGQKVYVVEVICDSVQF
                     LETRAARERQPQPQQQMQQPYQQPQNNDNFYDMKTVELEKEFDNSINTYDIMEDDIQ"
     gene            complement(495..776)
                     /gene="rpsF"
                     /locus_tag="PLK68_05290"
     CDS             complement(495..776)
                     /gene="rpsF"
                     /locus_tag="PLK68_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRM89861.1"
                     /translation="MNKYEIMFIVKPDVEDDARNALIESFKGILTANGGTVDNVDEWG
                     LRDLAYEIKDYKKGYYVVVDATCTAADVAEFERLSNINASMLRHLTLRK"
     gene            complement(911..2296)
                     /locus_tag="PLK68_05295"
     CDS             complement(911..2296)
                     /locus_tag="PLK68_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM89862.1"
                     /translation="MKERNNNFITHKIIIIIVLLGLIMGALVYQLRLAGEGETTITAK
                     ELKGQIVDITHETISLRDDNNIVYTVDCQKAKINGDELQYGNLVTIKYTGKLEQTTAI
                     QAIDVLGLNVQAVQVRNGGTGNTDATIASHKIAAMVEKMTLEQKIAQLFLARCPESQA
                     VELLSQYQLGGYMLYNRDFHNRTREEVIENIQSYQKAVTIPMLIAVDEEGGTVVRVSN
                     NLRSNKFRSPQDVFKAGGMDAIISDATEKSEFLKEFGINVNIGPVADVAMSKDDFIYQ
                     RSFGTDPNETAEFVKNVVKAMNDIKMGSVLKHFPGYGNVADNHTAICHDSRDYDSLVN
                     NDFLPFKAGISAGANSILISHIVVDSIDDQNLASLSPRVSKILRDDLNYHGVIIADDI
                     SMASAKAFGSEGEVALKAIKAGNDLIMTSNPQEHISALIAAAKNDEICLNSLDRSVMR
                     ILTWKSQLGIL"
     gene            complement(2297..2932)
                     /gene="ispD"
                     /locus_tag="PLK68_05300"
     CDS             complement(2297..2932)
                     /gene="ispD"
                     /locus_tag="PLK68_05300"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRM89863.1"
                     /translation="MNYSAIILCAGKGSRSGLDYNKMLYRFGDQTVYQMTMKTFLNDP
                     RCKQLVVVTKADEITDLKQLVTDKRIVYVYGGKERQDSVYNGLQVVDQDHVLIHDGAR
                     PYITTEKIDDLLLCLKEYDACLLMVPVKDTIKLCCDDTVEVTLPRARLMQAQTPQAFK
                     TSLIKVCYQQAQDSGFIATDDASLVEEFSTAPVKVVIGSYENIKITTPEDL"
     gene            3062..4006
                     /locus_tag="PLK68_05305"
     CDS             3062..4006
                     /locus_tag="PLK68_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRM89864.1"
                     /translation="MASLGVFLYSMHLMSTSLDTLANDKLENILYKLSSNKYLGVITG
                     TAITAIIHSSSATTIILIGLLNSHLISLNQATWIILGANIGTTVTGLMIALDLGQSAI
                     YLCVLGLLLMFLKNKIAYLGRVLMALGLIFLAMDSMAVALAPLQTSPYFINMFGHLDN
                     PLTAILVGTVFTALIQSSTASVGVLQTIYQKGLISFAMATNVIYGQNIGTCITAVLAS
                     LNGDRSSKRLSAIHILINVLGTIIFVILAKFIPLVSFIESLTPNYMMQIAYMHTFFNI
                     ISTIILLPFDNLLIYLANKVIPLSKQEVTYAHKPSRLY"
     gene            3978..4556
                     /locus_tag="PLK68_05310"
     CDS             3978..4556
                     /locus_tag="PLK68_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRM89865.1"
                     /translation="MLTNLLAYINTYGYKAIFLLIAIENLFPPIPCEIILTFGGFMTT
                     ITNLTPLGVITVASLGSYLGAVILYWFGYLINLERLEKLLVRFYFKRHDLSRSLNWFE
                     QYGKISVLIGRLIPIVRSLISLPAGITKMNFFSFSFYTLIGTIIWNSILVVLGIILGN
                     NWILISKYVKQYALIIAIITLLIILWKKRRLN"
BASE COUNT         1420 a          940 c          630 g         1656 t
ORIGIN      
        1 aactgaatat catcttccat gatatcatat gtattaattg aattatcaaa ttctttttct
       61 aattcaactg ttttcatatc atagaaatta tcattatttt gtggttgttg gtacggttgt
      121 tgcatttgtt gttgtggctg tggttgcctt tcacgagcag ctcttgtttc taagaattgc
      181 acactatcac aaataacttc tacaacatat accttttgac cttgagtatt gtcatagcta
      241 cgtgtttgga ttcttccttc aacaccaact aaacttccct tagaacaata tctctcaaca
      301 ttttcagcag gtttacgcca cacaacacaa ttaataaaat ctgcttgttg ttgaccatct
      361 ctgcttgtaa aattacgatt taccgctaaa gtaaaagaag taaccgcatc accttgtggt
      421 gttcttctaa gttcaggatc tctagtcatt cgtcctacta ggactactct attaatcatc
      481 tagtacgcct ccttctattt gcgaagagtt aaatgacgta acatagatgc attgatgttt
      541 gataatcgtt caaattcagc aacatcagca gctgtacaag tagcatcaac aactacatag
      601 tacccttttt tgtaatcttt gatttcataa gctaaatcgc gtaatcccca ttcgtcaacg
      661 ttatcaacag ttccaccgtt agcagttaaa attcctttaa agctttcgat taaagcgttt
      721 ctagcgtcat cttcaacgtc aggttttaca ataaacataa tttcatactt gttcatttct
      781 atacctccta tggtctattg gcttatctct ttggataagc aggcagtaaa tataatattt
      841 ttacatgctc aattataata gcacaccaat caaaacaaag caatgattta tttgattaaa
      901 tcatcatatt ttacagtatc cctaactgac ttttccaagt taagattcgc attacagaac
      961 gatccaacga attgagacat atttcatcat tcttagctgc tgcaataagt gccgagatat
     1021 gttcttgagg attagaagtc ataattaaat cattccctgc cttaatcgct ttaagcgcaa
     1081 cttcaccttc acttccaaag gcttttgcac tagccattga aatatcatca gcaataataa
     1141 cgccatgata atttaagtca tctcgtaaaa tcttactcac tctaggtgac aatgatgcta
     1201 gattttgatc atcaatacta tcaactacta tgtgactaat caaaatacta tttgcacctg
     1261 cagatattcc agctttaaat ggtaagaaat cattattgac aagcgaatca taatcacgtg
     1321 aatcatggca tatagcagta tgattatcag caacgttccc atatccggga aaatgtttta
     1381 aaacacttcc catcttaata tcattcattg cctttacgac atttttcaca aattcagctg
     1441 tttcattagg atcagttcca aatgaacgtt gatagataaa atcatcttta ctcattgcaa
     1501 catcagctac cggacctata tttacattaa ttccaaattc ttttaaaaac tccgactttt
     1561 ctgtagcatc actaataata gcgtccatcc cgcctgcttt aaaaacatct tggggagaac
     1621 gaaatttgtt actacgtaaa ttattactaa cccgtactac agttccgccc tcttcatcta
     1681 ctgcaatcaa catcgggata gtaactgctt tttgatatga ctgaatattt tcaattactt
     1741 cctcacgggt ccgattatga aaatcacgat tatataacat atagccacct aattgatatt
     1801 gtgacaacaa ctcaactgct tgactttcag gacaccgagc taaaaataat tgtgctattt
     1861 tctgctctaa cgtcatcttt tcaaccattg ctgcaatctt atgcgaagct atcgtagcat
     1921 ccgtattacc agttccacca tttctaactt gaaccgcttg aacatttaag cctaaaacat
     1981 caattgcttg tatcgcggtt gtttgctcga gtttacccgt atatttgata gttactaagt
     2041 tcccatactg taactcatca ccatttattt tggccttttg acaatccacc gtataaacaa
     2101 tattattatc atcacgcaaa ctgattgttt catgtgttat atcaactatc tgtcccttta
     2161 actctttagc tgttatcgta gtttcgcctt cccctgctaa gcgcaactga taaactaacg
     2221 cccccatgat caatcctaat aaaacaatga ttataatgat tttatgagta ataaaattat
     2281 tatttctttc cttcatttaa agatcctcag gagtagttat tttaatattc tcataactgc
     2341 ctattacaac tttgactgga gctgtactaa actcttcaac caatgaggca tcatcagtag
     2401 caataaaccc actatcttga gcctgttgat aacaaacttt aattaaactc gttttaaagg
     2461 cttgcggagt ttgagcttgc atcaatcttg ccctcggcaa agtcacctca acagtatcat
     2521 cacaacaaag tttgatcgta tctttaactg gtaccatcaa taaacaggca tcatattctt
     2581 ttaaacataa taataaatca tctatttttt ctgtcgttat atatggtcta gctccatcat
     2641 ggattaatac atgatcctgg tcaactacct gcaggccatt ataaacacta tcttgacgct
     2701 ctttacctcc ataaacataa acaattctct tatctgtcac taactgtttt aaatcagtta
     2761 tctcatccgc ttttgtaact acaacaagct gcttacagcg cggatcattt aaaaaagttt
     2821 tcatcgtcat ttgatacaca gtttgatcac caaaacgata taacatttta ttataatcca
     2881 gtccactccg gcttcctttt ccagcacata aaataatcgc actataattc atcaatatca
     2941 catcctttca ctcattgtaa catttaatga ttatcttgaa aacaaacatt atttatcgat
     3001 ttattaatat aattagtaat aataagggag tgattttatt actaaaaata taattggttt
     3061 tattgctagt ctcggtgttt ttctttatag tatgcatcta atgtcaacta gccttgatac
     3121 cttagcaaac gataagcttg aaaatatttt atataaacta tcttcaaata aatatttagg
     3181 tgttatcacc ggaacagcca tcacagcaat aattcatagt tcctctgcta caactattat
     3241 tctcatcggt ctccttaata gtcatctaat ttcattaaat caagcaactt ggattatttt
     3301 aggtgctaat atcggaacta ctgtaactgg actcatgatt gctttagatt taggtcagtc
     3361 agcaatctat ttatgtgtcc ttggattatt actaatgttc ttaaaaaata aaattgctta
     3421 tttaggacgt gtcttaatgg ccctaggatt gattttttta gcaatggaca gtatggccgt
     3481 agctctggca ccattacaaa catccccata ttttattaat atgtttggtc atttagacaa
     3541 tcctttaacc gctattttag ttggaacagt ttttacggcg ttgattcaaa gctcaactgc
     3601 atcagttggc gtcttgcaaa ctatttatca aaagggatta attagttttg caatggctac
     3661 caatgttatt tatggtcaaa atattggcac ttgtataacc gcagttttag catcacttaa
     3721 tggcgatcgc agttcaaaac gtttaagtgc tattcatatc ttaattaatg tactcggaac
     3781 tattattttt gtaatcttag caaaatttat cccactagtt tcattcatcg aatccttgac
     3841 tccaaattat atgatgcaaa ttgcttatat gcatactttc tttaatatca tttcaactat
     3901 tattctatta ccatttgata acttattgat ctatctggct aataaagtaa tcccattatc
     3961 aaaacaggag gtcacttatg ctcacaaacc ttctcgctta tattaacacc tacggctaca
     4021 aggcaatctt tcttttaatt gcgattgaaa atttatttcc gcctatccca tgtgaaataa
     4081 ttttaacttt tggcggtttc atgacaacga tcacaaacct aacacctcta ggcgtaatta
     4141 cagttgcttc gcttggttct tatttaggcg cagtgattct atattggttt ggctacttaa
     4201 tcaatctaga acgactcgaa aagcttctag tccgatttta ttttaaacgt catgatctat
     4261 cccgttccct taactggttt gaacaatatg gaaaaatctc tgttcttatt ggtcgcttga
     4321 ttccgattgt tagatcattg atttctctgc ctgccggaat tactaaaatg aacttcttta
     4381 gtttctcatt ttatactttg attggtacaa tcatttggaa tagtatttta gtagttcttg
     4441 gaattatttt aggaaataac tggatcttaa ttagtaaata tgttaaacaa tacgcactga
     4501 ttattgcaat catcacacta ttaattattt tatggaaaaa gcgaagactc aattaacttt
     4561 gggccttcgc tttattttga aaatatttat tagctgcttt aacaattcct cttccttgaa
     4621 gtttcatatc atattccttt aaaatc
//
LOCUS       DAOMTA010000147         4628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_503_length_4628_cov_2.156571, whole genome shotgun sequence.
ACCESSION   DAOMTA010000147 DAOMTA010000000
VERSION     DAOMTA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_4628_cov_2.156571"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..333)
                     /locus_tag="PLK68_05315"
     tRNA            complement(249..333)
                     /locus_tag="PLK68_05315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Leu,seq:cag)
     gene            907..2346
                     /gene="pepD"
                     /locus_tag="PLK68_05320"
     CDS             907..2346
                     /gene="pepD"
                     /locus_tag="PLK68_05320"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HRM89866.1"
                     /translation="MAVLDTEILVNYYFEEICKIPHGSYNEEKIADFVEAFAKEKGFR
                     YHRDHLNNIVIYKEASQGYENHETLMLEAHMDMVNEKNKDSNHDFEHDPLDLYLEDGY
                     VSANGTTLGADDGYGVAYMLAILSDPNVKNPPLECVFTVAEEVGLDGALGFDASLLKA
                     TRMIGLDSENEGEICTTSSGGCDVMITKELYFTSNENPTYTLLIKGLSGGHSGGEIHR
                     GKGNANKLAARVMYGMIKANLDIQLVDLNGGLKNNAIPRECAIVFASTSDFKLLQEVA
                     DEYQDYFSEEFEISDPGVKVELSLNNDIAQQTLSIKESKDIIKLMMAAKSGFVERSLV
                     IEDLTTVSLNMGVVSIKDKQLKIDYLLRSPMKSAVMNMVDELDIIADAFGGTITPANY
                     YPGWNYDQHSKLRDLFKAFYFKRTGAEVKEVATHGGLETGIFKGKMPALDIITMGPNM
                     ADIHTPDERMEVASFVNCYEILKDFIATL"
     gene            2455..3825
                     /locus_tag="PLK68_05325"
     CDS             2455..3825
                     /locus_tag="PLK68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89867.1"
                     /translation="MFKMKVDLTKDPILKSLLIFAFPLFVANVFQQLYNTMDTMIVGN
                     FLGDTSLAAIGACGAIYELLVGFALGVGNGLSIVTARSFGAKDENLLKKSVAGSIVIG
                     ILLTIILMLFSQFCLYPLLELLNTPANIINEAYDYIFMITIFVGVMFAYNLCAGLLRA
                     IGNSVMPLVFLLISSVLNVGLDLLFITQFNMGIQGAAIATVIAQGVSAILCFYYIYKK
                     CPILLPHKEHFKISRELYQELAGQGFSMGLMMSIVSTGTVILQTAINKFGYLIIAGHV
                     TARKLNSFCMMPAATLGLALSTFVSQNKGANQGLRIRQGVRYANLIAVGWSVIATVVL
                     FFIAPTLVQLLSGSSSAVVIDNGSLYLMLNAPFYCMLGILLNLRNSLQGLGRKIIPLV
                     SSIIEFIGKIVFVWLFIPLLGYFGVIICEPVIWCCMCLQLAYSFYRDPYIKEYRNIKK
                     EAVTGK"
BASE COUNT         1525 a          578 c          857 g         1668 t
ORIGIN      
        1 aacttggatt cattgtttta tatgttccta aaattccttg atttatatat agttagtttt
       61 tctattttaa gaaggctgat cttcgtgttc taatcatatt tacttacctc gcttttctta
      121 tattctatca gatagtaacc tagagtaaaa gttttttcac tttagtgtga gtttagcagg
      181 gggctttagg ctattaaata aatgcaaaaa agtttgtaga taacaatcga caaactttaa
      241 attaattatg gtacaggaaa tgggacttga acccacacga gaaaatctca ctagaacctg
      301 aaactagcgc gtctgccatt ccgccactcc tgcattatta ataagtacaa taattgttga
      361 gaagatcttg agataatttg gttgcatgga ggatagttaa tattgagaaa aaagagtaag
      421 caaaaacata tgttttaaaa acaaaaacca ttactaaacc aatgaatcct tctacaagaa
      481 atataattaa ataaatgtaa gggaggcagg ttattaaaaa tggtttgcta atagcaatgt
      541 tttttaacaa actgaatttt tttagtatta gcaagacaat atataatcca tagctagata
      601 ttgaccataa aactgttcta aagatcaaat caaaaagaat tgtttctatt ttgaaaaaag
      661 gatagcacca aatataacct agacacatta taatcaaata aatactgctt agtattaatg
      721 cctgtctttt cattggctag tcctcctaaa agctatacat gattgtgata tttaatgaca
      781 ggttaatcta aattaaaata attattaaag tcctttaata atattattat acatcattta
      841 atttgatttg ttaattagtt aaacattttg ttataataaa aatagtgaaa atggaggtta
      901 ggtataatgg ctgttttaga cacagagatt ttagttaatt attattttga ggaaatttgt
      961 aaaattcccc atggctctta taatgaagaa aagattgctg actttgttga agcattcgcg
     1021 aaagaaaaag gttttagata tcatcgggat cacttaaata atattgttat ttacaaagaa
     1081 gctagtcaag gatatgaaaa tcatgaaaca ctgatgttgg aagcacatat ggatatggtc
     1141 aatgaaaaga ataaagatag taatcatgat tttgaacatg atccattaga tttgtatctt
     1201 gaagatggct atgttagtgc taatgggacg acattaggag cagatgatgg ctatggagtt
     1261 gcttatatgt tggcaatctt aagtgatcct aatgttaaaa atccaccttt agagtgtgtt
     1321 tttacagttg ccgaagaagt tggtcttgat ggagcgttag gttttgatgc aagtttatta
     1381 aaggcgacaa gaatgattgg gctagatagt gaaaatgaag gagaaatttg cacaacttca
     1441 tcaggaggct gtgatgtaat gattactaag gaattatatt ttactagtaa tgaaaatcca
     1501 acttatacat tattaataaa aggattatca ggaggacatt ctggaggtga aattcatcgc
     1561 ggtaaaggaa atgccaataa attggctgct cgagttatgt atggtatgat caaggcaaat
     1621 ttagatatcc agctagtgga tctaaatggt ggattaaaaa ataatgcgat tccccgtgaa
     1681 tgtgcaattg tttttgctag tacaagtgat tttaagttac ttcaggaagt ggcagatgag
     1741 tatcaagatt attttagtga agaatttgaa attagtgatc ctggtgttaa agttgagtta
     1801 tcattaaata atgatattgc acaacaaacg ttgagtataa aagaatcaaa agatattata
     1861 aaattaatga tggcagctaa gagtggtttt gtcgaacgtt cattagtgat tgaagatttg
     1921 acaacggttt cattaaatat gggtgtagta tcaattaaag ataaacaatt aaaaatagat
     1981 tatttactac gttcaccaat gaaaagcgct gtgatgaaca tggttgatga attagatatt
     2041 attgcagatg cctttggtgg cacaattact ccagcaaatt attatcctgg ttggaattac
     2101 gaccaacatt caaaattacg tgatttattt aaagcatttt attttaaacg tactggagca
     2161 gaagtaaaag aagtagctac tcatggtgga ctagaaacgg gaatctttaa agggaagatg
     2221 ccagcgctag atattattac gatgggtcct aatatggcag acatccacac tccagatgaa
     2281 cgtatggaag ttgcatcatt tgtaaattgt tacgaaatat taaaagattt tattgcgaca
     2341 ttataaacca agtgttaact tggttttttc attctttgaa aagggatatt gttgaaaagt
     2401 ggcgataata ggaatacaat aatatgagtt ataatagata aaagagggaa aattatgttt
     2461 aaaatgaaag ttgatttaac caaagatcca attttaaaat cattgttgat ctttgcgttt
     2521 ccattatttg tggctaatgt ttttcaacaa ctttacaata ctatggatac gatgatcgta
     2581 ggaaactttt taggggatac atcattggcg gcaattggag catgtggtgc tatttatgaa
     2641 ttattagttg gttttgcttt aggggtagga aatggattaa gtattgttac agcacgaagt
     2701 tttggtgcta aagatgagaa cttgttaaaa aaatcagttg cgggatcaat cgtaatagga
     2761 atcttgttaa ctattatttt aatgcttttt tcacagtttt gtttatatcc gctattagaa
     2821 ttattaaata caccagcaaa tataattaat gaggcatatg attatatttt tatgattact
     2881 atttttgttg gggtaatgtt tgcttataat ttgtgtgctg gattattgcg agcaattggc
     2941 aatagtgtaa tgccgcttgt ttttttactt atttcttcag tcttgaatgt aggattagat
     3001 ttattattta ttactcaatt taatatggga attcaaggtg ctgctattgc aactgttatt
     3061 gctcaaggag tttcggcaat tctttgtttt tattatattt ataaaaaatg tcctattttg
     3121 cttcctcaca aagagcattt taaaattagc agggaactat atcaagaatt ggcagggcaa
     3181 ggtttttcga tgggattaat gatgtcgatt gtttctacag gaactgttat tttgcaaacc
     3241 gccatcaaca aatttggtta tttaattatt gctggacatg ttactgctcg taaattaaat
     3301 agtttttgta tgatgcctgc agcaacattg ggtctagccc tatctacttt tgtttcacaa
     3361 aataaagggg caaatcaagg attgcgaatt cgacaagggg tccgttatgc taatttaatt
     3421 gcagtagggt ggagtgttat tgcaacagtt gtattattct ttattgcccc aactctagtt
     3481 caattattgt cagggtcaag tagtgcagta gtaattgata atggttcatt atatttaatg
     3541 ctaaatgctc cattttattg tatgttagga atcttattga atcttagaaa ttctttacag
     3601 ggattggggc gtaagattat tcctttagtt tcaagcataa ttgaatttat tgggaaaata
     3661 gtatttgttt ggctgtttat tccattgtta ggatattttg gggttattat ttgtgaaccg
     3721 gtaatttggt gttgtatgtg tttacagtta gcttattctt tttatcggga tccatacatt
     3781 aaagaatata gaaatattaa gaaagaggct gtaacgggta aataattatg ttgtggtttt
     3841 gtcttgtgca aaaagcactc tatgatgaac gcttttttca gacttttgac aaagtgatac
     3901 tccaaaacga gtatcgcttt ttttatattg actaacttat agaaaaatag cgtttttgga
     3961 ttacatacag tgttgttccc ctatcttgct attattttta gaatgtgata cgcaaaaatc
     4021 attagtcctt gatgctgatt ctccttaagc cctttttact cttgtatatc tcaaattgtg
     4081 atgttttttg caattctatg gatttaattt gtatttccta tatcctaact tatttgtagc
     4141 gctgtattct aaaatcttta tgttgggaca tacatattca tattttttaa tgtagctttt
     4201 tttgtgattg atcttttata tggtgcatac atcttttgtt tgttttctaa tatcgtctta
     4261 caaattgtca gtgtgatata ttctggattt aaacatatat tttttgtcct atctttatat
     4321 tcttcattta gtaacgcata cgttggaaaa acgatacact gttatgcacg ttgcctgtga
     4381 cgcaatagtt taatccaacc aatctgtttt tatcacaaaa tgtttgatgg gaatatacta
     4441 ttcatataga tatgccggga aaccgtaaca gctgtttcaa aatagatcct gatgtaacat
     4501 ttatgcatat aaaatacgat tactataagc atacaaatgt atttaaatct gaatataata
     4561 tccaatttgg gataagtgat ggcattataa gaattatata tatcagcagt aacggtaatg
     4621 atttaaaa
//
LOCUS       DAOMTA010000148         4626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_504_length_4626_cov_2.066944, whole genome shotgun sequence.
ACCESSION   DAOMTA010000148 DAOMTA010000000
VERSION     DAOMTA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_504_length_4626_cov_2.066944"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1376
                     /locus_tag="PLK68_05330"
     CDS             219..1376
                     /locus_tag="PLK68_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM89868.1"
                     /translation="MEQILIKNFGPIEEVDIKDIRKVTIFIGESGSGKSTIMKVVSLF
                     RWIYKMVNIRSFLKYSGIKKSPFRFLFINLLKNSSMLGYLKPDTVLEYHNGSCTLTWD
                     PKTKKLKGTSVYIPKEELSLEKISFISDKRNLISDLVDNNTSIKRNMFYPGETYSDFE
                     TAVREVKEIDVLNLGVRFTVKKTSQGLKYTIVSETESDGFGIKLNEASSGTQNLVPMN
                     VIVEYYSKYYNLVSSINNAILSYVSRSDSLSDFKAVKNVGEFANKNVHLIIEEPELSL
                     YPSSQRSLMDFLIKNIYGDVQREYNISLMMATHSPYIVNSLNVLLRRGRIENNKQASI
                     KEDDISVFKVYHGRIQNLIAKDSKNGETIVNTMDLSEEMESIFKEYVSLRR"
     gene            1382..2038
                     /locus_tag="PLK68_05335"
     CDS             1382..2038
                     /locus_tag="PLK68_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89869.1"
                     /translation="MRDLLETELAASYGSQQMISVQEDTKSGLFGVNDPKACSDCINR
                     TSYFYRSCEDKKVSKHKSKKLVKIICLEDVFQQFRNVPSLTKGGCCDILMYSDDKVVV
                     MDMTCTRPEYLETHKKDGQEVEGKRDIVYRQIEDSVRKLRGCDVINTKLDSFTDRVAI
                     FAVRKKIFAVEESETDAAMGMKSYSKMSDDFSSKMGLHTDMGNGFFFIVQEYPKVFEW
                     "
     gene            2057..3907
                     /locus_tag="PLK68_05340"
     CDS             2057..3907
                     /locus_tag="PLK68_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89870.1"
                     /translation="MEVNVKQAQKIFFSKSSFEMIYFEAFVNALDAKATEFTIKIDLP
                     NKDQTLNLSLELLDNGVGFDNVRFSKFCKLFDVEEQSHKGLGRLVYLCYFDQVHVESF
                     FEKTKKRIFDFNENFNEDCELFDVPETNSRTALKLTSFSGNRLAKNQFIDPLYIKKVL
                     LENFYMKFYKAKLAERPINVVIQLTNGSVFSEQKISVADLPEFQKEPLTDQMNLFDKM
                     ELYYFIKEVPPAEHDVITAIAVDDRSMKVDIIANENLPVNYQMIFLLISESFKGDSDS
                     TRMNLTMDDVSMSRVRSIFKAAIASVVKKEIPKVEEANKQRINYLINTFPHLKGYFET
                     DDVGYSSQTDVLKKAQDHFFKDQREILCAKDLTDEQFEKSLNLSARSLAEYILFRQNV
                     IKKMKSLTGDNKEADLHNLIAPKNSEFMEGELSKDLYSNNVWVLDDKFMSYCTVLSEA
                     EMSKVIDVITKGEVKDNDDDRPDITLFFSADPNKGGNMVDIVVVELKRLGIKAEQNSI
                     VEFQLDTRTQRLAEYYGKRIQRMWFYGIVDFDNRYEMHLINNGFRPLFSKGSVYFRSK
                     PIFTDKSQSFNVVQNTYILDFKALVEDANSRNSTFLSILQNQFVAIKKNS"
BASE COUNT         1598 a          661 c          892 g         1475 t
ORIGIN      
        1 gtctgagcag cttttctgat gtagtgatac ctgccattat atatttcctg attgctttga
       61 tggatagtct tgactatttt aatatgtttt attttgatag tttaatgtta tttcttttgt
      121 cgtcatgaaa taaatttata actttgtagt ttataggaat gtccattgtg tgctattaat
      181 agaaaaatag gtggtggaca cataaaatag tttgttgtat ggaacaaata ttaataaaaa
      241 actttggacc gattgaagag gtcgatatta aagatatacg taaggtcacc atatttattg
      301 gtgaatctgg aagcggaaag agtaccatca tgaaagtggt atcacttttc cgttggatat
      361 acaagatggt aaacataaga tctttcttaa aatattccgg tatcaaaaag tcaccatttc
      421 gttttttgtt catcaatctc ctgaaaaata gtagtatgct tggctatctt aaaccagata
      481 cagttcttga atatcataac ggctcgtgca cattgacatg ggatccaaag acaaaaaaac
      541 ttaaaggtac ttctgtttat attccgaaag aagaactaag tcttgaaaaa atatcattta
      601 tctctgacaa aagaaatctc atttctgact tagttgacaa taatacatcc atcaaaagaa
      661 atatgttcta tcctggcgag acttattctg attttgaaac agcggtaaga gaagttaagg
      721 aaattgatgt acttaacctt ggcgtacgct ttactgtaaa gaagacatct caaggattaa
      781 aatatacaat tgtctccgaa actgaaagtg atggttttgg tatcaaactt aatgaagcat
      841 cttcgggtac acaaaatctg gtaccgatga atgtcattgt ggagtattat tcaaaatatt
      901 ataatctggt ttcctccatc aataatgcaa tactttctta tgtatctagg tctgacagcc
      961 ttagcgattt caaggctgta aagaatgtag gagagtttgc caataagaat gttcatctta
     1021 ttattgaaga acctgaactc agtctttacc cttcaagtca gaggtcatta atggattttc
     1081 tgattaagaa tatatatggt gatgtgcaac gggaatacaa tatttcgtta atgatggcca
     1141 cacatagtcc ttatattgtc aactctctga acgtccttct gcgcagaggc aggattgaga
     1201 acaacaaaca agcttcaata aaagaagatg atatttccgt attcaaagta taccatggtc
     1261 gaatccagaa tctgatagct aaagattcaa aaaatggcga aacaatcgtc aatacgatgg
     1321 atttgagtga ggaaatggaa agcatcttta aggaatatgt atcgttgagg agataggtaa
     1381 aatgagggac ttattggaaa cggaattagc tgcttcttat ggcagtcaac aaatgatatc
     1441 agttcaagaa gacactaagt ccggcctttt cggagtgaat gatcctaagg catgtagtga
     1501 ttgcataaac cgtacttctt acttttatcg ttcttgcgag gataaaaagg tttctaaaca
     1561 taaatcaaaa aaacttgtaa aaataatttg tctggaggat gtatttcagc agtttaggaa
     1621 tgtgccctct cttacaaaag gcggttgttg tgatatactg atgtattcag atgataaggt
     1681 tgtcgtgatg gatatgactt gcaccagacc ggaatatctt gagacacaca aaaaagatgg
     1741 tcaagaggtt gaaggtaaac gtgatattgt ctatcggcag atagaagatt ccgttaggaa
     1801 gttaagagga tgtgatgtta ttaacacaaa actggattcc tttacagacc gtgtagcgat
     1861 ctttgctgta aggaaaaaga tatttgcagt ggaagaaagc gaaactgatg cggctatggg
     1921 aatgaaaagt tattctaaga tgtctgacga tttcagttcc aaaatgggat tacatactga
     1981 catggggaat ggtttctttt tcattgtcca agaatatcct aaagtttttg agtggtaatg
     2041 ttcaaaaaaa taatttatgg aagttaatgt taaacaagca cagaagattt tcttctcaaa
     2101 gtcatccttt gagatgattt actttgaggc atttgtaaat gcattggatg ccaaagccac
     2161 tgagttcaca ataaagattg acctgccaaa caaagatcaa acattgaatc tttctttgga
     2221 actactagat aatggagttg gttttgataa tgttagattt agtaaattct gtaaactttt
     2281 cgatgttgaa gaacagtctc ataagggact gggacgtctc gtttatttgt gttactttga
     2341 ccaagtacat gtagagagtt ttttcgagaa aacaaaaaaa cgtatttttg attttaacga
     2401 aaactttaat gaggattgtg aacttttcga tgtacctgaa acaaacagtc gaacagctct
     2461 caaacttaca tcattctctg gaaataggct tgcaaaaaat caatttatag atcctttata
     2521 cataaaaaaa gtattacttg agaatttcta tatgaagttc tacaaagcca aattggcaga
     2581 aagacctatt aatgtagtga ttcagttaac caatggaagt gttttttcag aacaaaagat
     2641 ttcagttgct gacttacctg aatttcagaa agaaccctta acagaccaga tgaatttgtt
     2701 cgataaaatg gaattatact attttatcaa agaagtccct ccagcagaac atgatgtcat
     2761 tactgctatt gctgttgatg atagaagcat gaaagtcgac ataatagcga acgaaaatct
     2821 acctgttaat tatcagatga tatttctgct tatatctgaa tcctttaaag gagattcaga
     2881 tagtacgaga atgaatttaa caatggacga tgtttcgatg tctcgggtta gatctatctt
     2941 taaggcagcc atagcatcag ttgttaaaaa agaaattccc aaagtggaag aagcaaataa
     3001 gcaaagaata aattacttga taaatacttt tccacatctt aagggatatt ttgagacaga
     3061 tgatgtgggc tattcgtctc agactgatgt cctcaaaaaa gctcaagatc atttttttaa
     3121 ggatcaaaga gagatattgt gtgcaaaaga cttaacagat gaacaattcg aaaagtctct
     3181 taatctctca gctagatctt tagctgaata cattctgttt cgtcaaaatg ttatcaagaa
     3241 gatgaagtct ttgactggcg acaacaaaga agcagactta cacaatctta tagcaccaaa
     3301 aaacagtgaa tttatggaag gggagttgtc taaagattta tacagcaaca acgtttgggt
     3361 acttgatgat aaattcatga gctattgtac ggttctcagc gaggcagaaa tgtcaaaggt
     3421 cattgatgtt attacgaaag gggaagttaa agataatgat gatgacagac ctgacattac
     3481 attgttcttc tctgcagatc ctaataaagg aggaaatatg gtagatatag ttgtagtgga
     3541 gttaaaaaga ctaggcataa aggcagagca aaactctatt gtagagtttc agctggatac
     3601 cagaactcag cggttggcag aatactacgg aaaacgaata caacgaatgt ggttttatgg
     3661 aatagtagat tttgataata gatatgaaat gcatttaata aacaatggat ttcgaccatt
     3721 attctcaaaa ggtagtgtat attttaggtc aaagcccatt tttacggata agtctcaatc
     3781 ttttaatgtt gtgcagaata cctatatttt agattttaag gctcttgttg aagatgcaaa
     3841 ttcaaggaat tcgacattcc taagtatttt gcagaatcaa tttgtagcga ttaaaaaaaa
     3901 tagctaaatt aatcaaaata tttgaagctt gggtaaatag agattataaa atagaatact
     3961 gataagttga tgatgttgta tagtttataa ttatataagc aattgcacaa tttgttaggc
     4021 ttaatgtagt tctcaactaa gatgaagttt aaactataaa atatatttca cctccctttg
     4081 gcgaaattac acataactgt tttattatca gtattttgac tacggggagg tgaaaattta
     4141 acctccccat tatgcttttg gcaggcgaaa gcattgataa tatataccaa cttaacttaa
     4201 tttgcagatc aatctaactt aaattggtct gcaaattaag ttgaattgat tgccattatt
     4261 tatgctatca gggctgacac attaccattt agtaagtata tttcattata cataattaac
     4321 gtaatgtatt catatacagt catttaaata tttgtatata taaatattat ttactatctt
     4381 tgtacttgat ataaatgtaa aatctgtaaa gaatatggaa ttatttacga tattgaatga
     4441 agtaaaagac ccaagaaggg accatttgaa ggagcacagt ttagagtgta tcttttatta
     4501 ctatagcatc tgtgattggt ggtgcggaaa gctggtatga agtggaggag tttggaaaaa
     4561 tggaggagtc ttttttccgt tccaggataa aagacttcaa gggagttcct tctcatgata
     4621 cattta
//
LOCUS       DAOMTA010000149         4586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_512_length_4586_cov_1.946811, whole genome shotgun sequence.
ACCESSION   DAOMTA010000149 DAOMTA010000000
VERSION     DAOMTA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_4586_cov_1.946811"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1393
                     /locus_tag="PLK68_05345"
     CDS             20..1393
                     /locus_tag="PLK68_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM89871.1"
                     /translation="MKEREKFSSRLGFILISAGCAIGLGNVWRFPYVVGQYGGALFVL
                     IYLFFLIALGAPIMTMEFAVGRASQKSAALAFHSLVPKRKFWGAIGKFQMAGNYMLMM
                     FYTTVGGWMIAYFFKMIKGDFNGITPAQVGDIFNNSLQDPFMQVFWMIVVVVLGFGIC
                     SLGLQNGVEKITKIMMSSLLIIILILVIRAVTLPGGRDGLAFYLIPNLVAIKQYGLLE
                     IIFAAMGQAFFTLSLGIGAIAIFGSYIGKERRLFGETISVCTLDTLVALLSGLIIFPA
                     CFAFGVNPESGPGLVFITLPSIFNEMWLGQVWGCLFFVFMSFAALSTIIAVFENIISF
                     GMDLFNWSRKKAVVVNFIIILILSLPCALGFNILSDITPFGIGTTIQDLEDFLVTYNF
                     LPLGSLAYLLFCTCRYGWGFDGFLQEANTGTGIKFPKWTKNYLKFILPCVIIFIFIQG
                     YYTFFIK"
     gene            complement(1472..1981)
                     /locus_tag="PLK68_05350"
     CDS             complement(1472..1981)
                     /locus_tag="PLK68_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HRM89872.1"
                     /translation="MDSVILIIILIALVEASFKHVDIFNEFIEGVKEGSKLLITLFPT
                     MMAFTLWVTCFQYCGLIELLEIGFKSIFAILNIPIDIFMMMIVRPFSSNGSLTILNNI
                     FLKYGVDHPYSILGSIIQTGSDTTFYVVTLYFGSIKLQNNRYALKLGLWLDAIACLLA
                     TIAYLLFIG"
     gene            complement(1974..2546)
                     /locus_tag="PLK68_05355"
     CDS             complement(1974..2546)
                     /locus_tag="PLK68_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM89873.1"
                     /translation="MAKVFKFGLIFFVIIAFCTNNQNKMFEAILSSPQQVFDLIKVIV
                     LSACLWNGFLNIIKASGLIKQLSFLLKPILKLIYGNTVEDDQVYLYLSTNFIANLLGV
                     GSLASISGLKAMQSLTKYQRDPKTPCKEMMLLVILNTTGLSIIPTTMMTLRQSYGSHD
                     ILGFFGYSLTIGFIITIVGIIASKVIEHYG"
     gene            complement(2539..3546)
                     /locus_tag="PLK68_05360"
     CDS             complement(2539..3546)
                     /locus_tag="PLK68_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRM89874.1"
                     /translation="MKLGLIISLMMTLVNTNVSAVPFQGKSYIVMEASHQIVIEGSNQ
                     DYIQSVASISKIMTCIIAIENMELDTIITVDDTINKAWGSGVYIHIGDKISLRDLLYG
                     LMLRSGNDAAVMIAKAVGKEIPKFVDMMNDKAKELQLHSTTFSNPTGLDEEDNGNQST
                     VYDMARLMAYCHQNPIFNEIVSTKEYKREDGNGTWHNKNKLLTNYEYCIGGKTGFTKK
                     ARRTLITMARKDDLDLIIVTFNCGNDFEFHQKKFEECYANLKATKIFSKGIVEFKQQQ
                     YYLDFDVYVNKKPTDKVEVSLNNNEIVILLNNQPIAKQKVVPYNYFLGFKMVLKDLFY
                     G"
BASE COUNT         1523 a          627 c          781 g         1655 t
ORIGIN      
        1 aatagaaggg tttaatttca tgaaagaaag agaaaaattt tcatcaagat taggttttat
       61 cttgatatca gcagggtgcg cgattggact tggaaatgta tggcgttttc catatgttgt
      121 tggtcaatat ggtggggcac tatttgtatt aatttattta ttttttttaa ttgctttagg
      181 agcaccaatt atgacaatgg aatttgcagt cggacgtgca agccaaaaaa gtgcggcatt
      241 agcatttcat tcactggtac ctaagagaaa attttggggt gcaattggaa aatttcaaat
      301 ggctggaaat tatatgttga tgatgtttta tacaaccgtc ggaggctgga tgattgctta
      361 tttttttaaa atgataaaag gtgactttaa cggtattact ccagcacaag ttggagatat
      421 atttaataat tcgctccaag atccatttat gcaagtattc tggatgattg tagtggttgt
      481 tttgggattt ggaatttgtt ccttgggcct tcaaaatggt gtagaaaaga ttactaagat
      541 catgatgagt tcattattaa ttattatttt gattttggtg atacgtgctg ttactttacc
      601 tggtggtaga gacggtttgg cgttttactt aattcctaat ttagtggcaa ttaaacaata
      661 tggtctttta gagattattt ttgctgcaat gggacaagca tttttcactt taagtttagg
      721 aattggggca attgctattt ttggaagcta tattggcaaa gaacggcgtt tatttggtga
      781 gacaataagt gtatgtactt tagatacgtt agtggcatta ttatcaggct taattatttt
      841 tcccgcatgt tttgcttttg gagtaaatcc tgaaagtgga ccgggtctgg tatttattac
      901 gttgcctagc atttttaatg aaatgtggtt aggacaagta tggggatgtt tattttttgt
      961 ctttatgagt ttcgcagctt tatcaactat aattgcagta tttgaaaata ttatttcatt
     1021 tgggatggat ttatttaatt ggagtcgtaa aaaagcagtt gtagttaatt ttattattat
     1081 tttaatttta tctttacctt gtgcccttgg ctttaatatt cttagtgata ttactccatt
     1141 tggtatcgga acaacaattc aagatcttga agatttcttg gttacatata atttcctgcc
     1201 acttggttca ttagcgtatt tgttattttg cacatgtcgt tatggctggg gatttgatgg
     1261 ttttctccaa gaagctaata caggaacagg aattaaattc ccaaaatgga caaaaaatta
     1321 tttaaaattt attcttcctt gtgtgattat ttttatattt attcaaggat attatacgtt
     1381 tttcattaaa taattgtttg ataaaaggtg ttgctttaag taacaccttt tatgcaagtt
     1441 atttgttgaa ttttgataat atttgattgt tctaaccgat aaagagtaaa tatgcaatag
     1501 ttgcaagaag gcaagcaatc gcatcaagcc aaagacctaa ttttaaggcg tatcgattat
     1561 tttgaagttt tatactgcca aaatacaagg ttacaacata aaatgtagta tcgctgcctg
     1621 tctgaataat ggagccaaga atgctataag gatgatcaac cccatacttt aaaaagatat
     1681 tatttaaaat agttagtgat ccatttgaag aaaatggtct aacaatcatc atcataaaga
     1741 tatcaatagg aatattcaaa atagcaaaaa tactcttaaa accgatttct agtagttcaa
     1801 ttaggccgca atactgaaaa catgtgaccc atagtgtaaa agccatcatt gttggaaata
     1861 aagtaattag gagtttactg ccttccttta ccccttcgat aaattcatta aaaatatcaa
     1921 cgtgtttaaa acttgcttcg actagagcta ttaaaatgat aattaagatt acactatcca
     1981 taatgttcaa tcaccttact tgcaataata ccgacgattg taatgatgaa accaatagta
     2041 agactatagc caaagaagcc aaggatgtcg tggctgccat aactttgtct taacgtcatc
     2101 attgttgtag gaatgattga aaggccagta gtgtttaaaa taactaaaag catcatttct
     2161 ttgcatggag tctttggatc acgttggtat tttgtcagag attgcattgc ttttaaacca
     2221 ctaatagaag ccaaagaacc aactcctaat aaattagcaa taaaattagt tgagagatat
     2281 agataaactt gatcatcttc gaccgtatta ccatagataa gttttaaaat cggcttaagt
     2341 aaaaacgata attgtttaat aagtcccgaa gctttaataa tatttaaaaa cccattccaa
     2401 agacaagcac ttaaaacgat tactttgatt aagtcaaaaa cttgttgtgg tgaacttaaa
     2461 attgcttcaa acattttatt ttgattatta gtgcaaaaag caattatcac aaagaaaatt
     2521 aaaccaaatt taaaaacttt agccatagaa taaatccttc aggaccattt tgaaacctaa
     2581 aaagtaattg tatggaacaa ctttttgttt agcaattggc tggttattta ataaaatcac
     2641 aatttcatta ttatttaatg aaacttcaac tttatcagtt ggttttttat taacatagac
     2701 atcaaagtct aaatagtact gttgctgttt aaattcaacg ataccttttg agaatatttt
     2761 agttgctttt aaattagcat aacattcttc aaatttcttt tgatgaaatt caaaatcatt
     2821 tccacaatta aaagtaacaa taattaaatc aagatcatct ttacgagcca tggtaattaa
     2881 ggttcgtcgt gcttttttgg taaaaccagt ttttccacca atacaatatt catagttggt
     2941 taataattta tttttgttat gccaagtccc attaccgtct tcgcgtttgt attctttagt
     3001 actgacaatt tcattaaaaa ttggattttg atggcaatag gccattaatc gtgccatatc
     3061 ataaacagtg gattgattcc cgttatcttc ttcatctaaa cctgttggat tagaaaaagt
     3121 tgtgctatgc aactgaagtt cttttgcttt gtcattcatc atatcaacaa attttggaat
     3181 ttctttacca actgctttag caatcattac agcagcatca ttgccacttc taagcatcaa
     3241 accatatagt aagtcacgta gactgatttt atcacctata tgtatataga caccacttcc
     3301 ccaggcttta tttattgtat catcaactgt aatgatagta tcaagttcca tgttttcaat
     3361 cgcaataata caggtcataa tttttgaaat tgaagcaacg ctctgaatgt aatcttgatt
     3421 actgccttcg attacgattt gatgactagc ttccatgaca atataacttt ttccttggaa
     3481 gggtacagca ctgacgtttg tatttactag tgtcatcatt aaacttataa ttaatcctaa
     3541 tttcattttc ttcacccctt taaacatatg ttgaacttgg aaaaatagaa gttttagagt
     3601 agaaaataaa actgtcaaaa tatcctaata tttgtaatta taatattaat tgtattaaat
     3661 ctaggtaaga tatataacta taagcattga atttgtttca ataacccaaa atttaatatt
     3721 agtctttgtg gcatgaggag aatccctacc gatggttaca gttcatgacc catattttat
     3781 aatatggctg agttagtaaa attctaatat cgacggttaa agttcagatg agaaaagaca
     3841 ttttctagaa ttctatcgaa ggcattttat tatgttttcg gttttttatt ttaggaggaa
     3901 gaaagttgaa aagaggaaag acaaggtgat tagtgttgaa cgcaatttta ggggcattat
     3961 aggcgatgct gatggtattt tgattcccat tacctttaat accaccattt ttatattttg
     4021 atacagctta tgtttacagg tgaattacaa agttttatgg ttaagtattg ctttggttta
     4081 accagcagcc ttgatctatc gttatgataa aactaaaatt gttactttta aaggaacgtg
     4141 ggttgaaatt attattaatg taggagttgt tctctttaat taaaagtatt attgtatatt
     4201 tgattacatt attggtatat aaaaaactaa gtactataat tcatcatcat acaggtgact
     4261 aaaagaaaag agaggtgatt tttgaagatg gagctgtttt attttttaaa aaattaggaa
     4321 attggaaaat tatgtattag cgactagtgt agatgattat gtaacggttc gtaatattag
     4381 ttgtttattt taaaacagat aaaaatttta gaaaaactca acaattattt aaaaatcctc
     4441 aagttgcatt atgcaatgga agaattcaaa taaaagggct gacgaaaaat ataggcttag
     4501 tgattgaaaa actgggacgt aaatttgagc agttatatcg tcagtattta tgacaaaatt
     4561 acaatgcgta tagttatgaa aaacag
//
LOCUS       DAOMTA010000150         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_517_length_4575_cov_2.317257, whole genome shotgun sequence.
ACCESSION   DAOMTA010000150 DAOMTA010000000
VERSION     DAOMTA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_517_length_4575_cov_2.317257"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1040)
                     /locus_tag="PLK68_05365"
     CDS             complement(120..1040)
                     /locus_tag="PLK68_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRM89875.1"
                     /translation="MDNLFNVSTAPHIRSSITTIMYDVIISLLPATFFGIYQFGFNAF
                     LVITTAIITAVLSEYLYQRLMKLPITIKDGSAAITGLLLALCLSSSLPLWMVALGSIF
                     AVIIVKQLFGGLGQNFMNPALAARCFLLISFAGKMNSFVSIDASTSATPLAMIQNGQN
                     VNLLKMFIGNTSGTIGETSVIALLLGAIYLIYRKVISPKIPLCILFSFSLIILINNNF
                     NSLFLLQQICGGGLMIGAFFMATDYVTSPITPTGKIIYGLVIGILAAVFRLYGNTPEG
                     MSFAIIITNLLVPLIEKVTVPKIKGSGGKA"
     gene            complement(1040..2368)
                     /gene="rsxC"
                     /locus_tag="PLK68_05370"
     CDS             complement(1040..2368)
                     /gene="rsxC"
                     /locus_tag="PLK68_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRM89876.1"
                     /translation="MRILSFKGGIHPHDNKSLSANHPIKLLNPGDELIYPLSQHIGAP
                     AKAIVAKGDHVLVGQIIANGNGMISANVASSVSGTVKTIDLRLAPTGFFVDCIVITND
                     HQYQSIPEYNTTNDYHNYSSMQIRELIAHAGIVGLGGAGFPSAIKNTPKDDDSIKYVI
                     INGAECEPYLTSDYRLMLEETDKLIAGIKIQLQLFKNAKAIIAIEDNKPEALAHLKKV
                     VVDEDKIEIAALPTKYPQGSERVLIKVLTNQTIVNGMLPADVGCIVSNVASIIAIYEA
                     VALNIPLIEKIVTVTGDAIFQPQNFKVRLGTKYQTLVDACGGFKVHPQKIISGGPMMG
                     TALFNLDVPVSKTSSALVCLSEDEVSKTAPTACIHCGRCVDVCPIGLIPQLLYRYART
                     GDKENFLKVHGNDCMECGCCTFTCPAKRNMTQSFKKIKKAINDDRKKGAK"
     gene            complement(2496..3080)
                     /locus_tag="PLK68_05375"
     CDS             complement(2496..3080)
                     /locus_tag="PLK68_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRM89877.1"
                     /translation="METKTKTNLLRAFAGECQARMRYEMAAKVAGKNGMAILQDMLYF
                     TANQEKEHAIVFYNYLKDVFKDANVSMEANYPVDLDQDLSSLLEQAAQHEYDEATSIY
                     KQFGDEAKEEGYVEIATTFYMICEIEHYHHERFKKYHNLLTSGKLFNDQSTVKWMCMN
                     CGFIYEGTDALTICPVCKHPQGYSLRLDESQFHL"
     gene            complement(3217..3609)
                     /locus_tag="PLK68_05380"
     CDS             complement(3217..3609)
                     /locus_tag="PLK68_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HRM89878.1"
                     /translation="MQETVLLYNIDKTDAGKAIISILEKLNVEVIIVKSSDLMSPIGY
                     ILGADNFERGTEALTEIPQDDMMVMAGFEDKQVDLLLQIFKEANIPFIPLKAIVTQTN
                     VNWTFMQLLKNVKTEYMELTGMNKDMIN"
     gene            3709..>4189
                     /locus_tag="PLK68_05385"
     CDS             3709..>4189
                     /locus_tag="PLK68_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Mg2+ transporter"
                     /protein_id="HRM89879.1"
                     /translation="MRYYLEKEITNSYKEGSLVIDILSKEDFINQYQKENHANHVIRH
                     IGDIMYCKVELFYDVICGAFVIPDKKDLKIKHCFSFYLTEQKMIFIDDNDYVMKLIEK
                     MKDTYTNNRLSLNKFFHDLIFLITVDDGMYLQEYADRLQVIEDKIAFNFNSQINNEII
                     "
     assembly_gap    4190..4199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4242..>4575
                     /locus_tag="PLK68_05390"
     CDS             4242..>4575
                     /locus_tag="PLK68_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRM89880.1"
                     /translation="MIDILSDNESDFLNDYECHLFSMQARRIDRLDNQLNALKDYSLQ
                     IKEMYQNKIDTHQNKIMTVLTVATTIFFPLSIITGWYGMNFKNMPELNNPYGYIIVIG
                     ASILVVIIE"
BASE COUNT         1561 a          729 c          722 g         1553 t
ORIGIN      
        1 tttttttttt tgtctagcaa ttggttcttt ggtaatagaa ttaaccatag ctagagataa
       61 actagcagca actgtaatta caacaaaaac gagaatatct tttagaagat tttttttcat
      121 tatgctttac ctccactacc ttttatttta ggcactgtta ctttttcaat caaagggaca
      181 agtaaattag taatgataat agcaaatgac attccctcag gagtattacc atagagccta
      241 aaaacagcag ctaatattcc aataaccagt ccataaataa ttttaccggt tggagtaatc
      301 ggacttgtca catagtcagt agccatgaaa aatgccccaa tcattaaacc accaccacaa
      361 atttgttgta ataaaaatag actgttaaag ttattattga tcaaaatgat taaagaaaat
      421 gaaaatagga tacacaacgg tattttagga ctaattactt ttcgataaat taggtagata
      481 gcgcctaaaa gcagagcaat tactgatgtt tcaccaatcg taccactagt atttcctata
      541 aacattttga gaagattgac attttgtcca ttttgtatca ttgccagcgg agtagcacta
      601 gtacttgcgt cgatgctaac aaagctgttc atttttccgg caaatgaaat taataaaaaa
      661 catcgagctg ctaaagcagg attcataaag ttctgaccaa gtccaccaaa aagttgctta
      721 acaattataa ctgcgaatat acttcccaaa gcaaccatcc acaatggcaa tgagcttgat
      781 aggcataacg ctaataataa gcctgtgatg gctgcagagc cgtctttaat cgtgattggc
      841 agcttcatca atcgttgata caaatattca cttagaacag ctgtaataat agcagttgta
      901 ataacaagaa acgcattaaa tccaaattga taaattccaa aaaaagttgc tggtaataaa
      961 gaaatgatta cgtcatacat aatagttgta attgaactac gaatatgggg agcagtagac
     1021 acattaaata aattatccat tattttgctc ctttcttgcg atcgtcatta attgcttttt
     1081 ttattttttt aaatgattgt gtcatgtttc gctttgcagg acatgtgaag gtacagcagc
     1141 cgcactccat acaatcgttg ccgtgaacct ttaaaaagtt ttctttatcg cctgttctgg
     1201 cataacggta tagtaattgt ggaataagac cgatagggca aacatcgacg catcggccgc
     1261 agtgaatgca cgcagttgga gcggttttag atacttcatc ttcacttagg caaactaaag
     1321 cagaagaagt tttagaaact ggtacatcta aattaaataa agcagtaccc atcatgggac
     1381 caccagaaat gattttttga ggatgtactt taaaacctcc acaagcgtca acaagggtct
     1441 gatatttagt tcctaggcga accttaaaat tttgtggttg aaagattgca tcaccagtta
     1501 ccgttacaat cttttcaatt agtggaatat ttaaggcaac ggcctcatag atagcaataa
     1561 tagatgcaac attgctgaca atacagccaa catcagctgg caacatccca tttacaattg
     1621 tttgattagt taatacttta atcaatacgc gttcacttcc ttgagggtat tttgttggta
     1681 aggctgctat ttcaatttta tcttcatcta caacgacttt ttttaaatga gctaatgctt
     1741 ctggcttatt atcttcaata gcaataattg ctttggcatt tttaaataac tgtaattgaa
     1801 ttttgatgcc agcgattaat ttatctgttt cttctagcat taaacgatag tcagaagtta
     1861 aatagggctc acattctgca ccgttgataa ttacatattt aattgagtca tcatctttag
     1921 gggtattttt tattgcgctg gggaagccgg cgccacctaa accaacaatt cctgcgtgag
     1981 cgataagttc acgaatttgc atactgctat aattatgata atcatttgtt gtgttgtatt
     2041 ctggaatact ttgatattgg tgatcgttag taataacgat acaatcaaca aaaaaaccag
     2101 taggggcaag tcgtaaatca attgttttaa cggttccaga gacagaacta gcaacattgg
     2161 cagaaatcat accgtttcca ttagcaataa tttggccaac taaaacatga tcacctttag
     2221 caacgatagc tttagctgga gcaccaatat gctgtgataa tggataaatc agttcatctc
     2281 caggatttaa tagtttgatt ggatgattgg cggaaagact tttattatca tggggatgga
     2341 ttccgccttt aaaacttagt attctcattt gaattaaact ccttttttgg actatatttt
     2401 aattatagac tttttataat taatattaaa tagtttagac aagttaaaaa attaacttaa
     2461 aaagcaatcc tttcaagagg attgctttta ctagtttata aatgaaattg agattcatca
     2521 agacgtagac tataaccttg tgggtgttta caaactgggc agatcgttag tgcatcagta
     2581 ccttcataaa taaaaccaca gttcatacac atccatttta cagtagactg gtcattaaat
     2641 aatttaccac tggttaataa attatgatat tttttaaaac gttcatggtg atagtgttca
     2701 atctcacaaa tcatgtaaaa agttgttgca atttcaacat aaccttcttc tttagcttca
     2761 tcaccaaatt gcttgtagat agatgttgct tcatcgtatt cgtgttgtgc tgcttgttct
     2821 aataacgaac ttaaatcctg atctagatca acaggataat tagcctccat tgaaacatta
     2881 gcatctttaa aaacatcttt aagataattg taaaaaacta tcgcatgctc tttctcttga
     2941 tttgcagtaa aataaagcat atcttgtaaa atagccattc cattcttacc tgcgacttta
     3001 gcagccattt cataacgcat tcgagcttga cattcaccag caaaagctct taataaattt
     3061 gttttggttt ttgtttccat atataagtta cctccacttt tatttttaac aaaataaaag
     3121 aagattaaac ttaatcttct aagaatcaaa taatctcttt aataataatt aaaatattat
     3181 aacgctgata tagatactgc taacaagaca aataatctag ttgatcatat ctttattcat
     3241 tccagttaat tccatatatt cagttttaac atttttcaat aattgcataa atgtccaatt
     3301 tacatttgtt tgagttacaa ttgcctttaa cggaatgaaa ggaatgtttg cttctttaaa
     3361 aatttgtaat agcaaatcaa cttgtttatc ttcaaaacca gccataacca tcatgtcatc
     3421 ttgtggaatc tctgttaatg cttcagttcc acgttcaaaa ttatcagcac ctaaaatata
     3481 cccaatcggg ctcattaaat cacttgattt aacaatgatt acttcaacat tcagtttttc
     3541 aagaattgaa ataattgctt taccagcatc agttttatca atattgtata ataatacagt
     3601 ttcttgcata tgtattgcct cgctttcgtt taataggtta tcatatttat ggttaacgtt
     3661 caactaagtt gacttaatgt gctataattt atccgggtag gtgagataat gcggtattat
     3721 ttagaaaaag aaattacaaa tagttataaa gaaggaagct tagttattga tattcttagt
     3781 aaagaagatt tcataaacca gtatcaaaag gaaaatcatg caaaccatgt gattaggcat
     3841 attggggata ttatgtactg taaagtagaa ttattttacg atgtgatttg cggagcgttt
     3901 gttatacctg acaaaaagga tttaaaaata aaacattgtt ttagttttta tttaacagaa
     3961 caaaaaatga tttttattga tgataacgat tatgtgatga aacttattga aaaaatgaaa
     4021 gatacatata ctaataatcg tctaagtttg aacaaatttt ttcatgattt gattttctta
     4081 ataactgttg atgatggaat gtatttacaa gaatatgctg atcgtttaca ggtcatcgaa
     4141 gacaaaatag cttttaattt caatagtcaa ataaataatg agataatctn nnnnnnnnng
     4201 aactattaat tttaaattcc tattataatc agttggggga tatgatcgat attttaagtg
     4261 ataatgaatc agatttctta aatgattatg aatgtcattt attcagcatg caggcacgta
     4321 ggattgatcg tttggataac cagttaaatg ctttaaaaga ttattctttg caaataaagg
     4381 aaatgtatca aaataaaatt gatacccacc aaaataaaat tatgactgtt ttaacagttg
     4441 caacaactat tttcttcccg ttatcgatta tcacaggatg gtatggaatg aattttaaaa
     4501 atatgccaga gttaaataat ccttatggtt atattattgt tattggagca agtattttag
     4561 ttgtaattat tgaaa
//
LOCUS       DAOMTA010000151         4561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_521_length_4561_cov_2.135819, whole genome shotgun sequence.
ACCESSION   DAOMTA010000151 DAOMTA010000000
VERSION     DAOMTA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_4561_cov_2.135819"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..>541
                     /locus_tag="PLK68_05395"
     CDS             279..>541
                     /locus_tag="PLK68_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM89881.1"
                     /translation="MKVKNSVEQAICLLIIIAHSNDEKPLKSYNISDSLGVSDSYLKK
                     IIRQLVVAGLITSEAGKKGGVCLSRKPDKITLLDIFEAIEGKEP"
     assembly_gap    542..551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(745..951)
                     /locus_tag="PLK68_05400"
     CDS             complement(745..951)
                     /locus_tag="PLK68_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89882.1"
                     /translation="MKEKSSVFMTLGTAVGLTIAVIIFVIGFVNKETSSWSALWFIVF
                     AFIGRVIGYGLDRIVDKNNQKKHG"
     gene            1046..1699
                     /locus_tag="PLK68_05405"
     CDS             1046..1699
                     /locus_tag="PLK68_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM89883.1"
                     /translation="MINTIIFDLDGTLIDSLVDLSNTVNVILTEKGYPTHTLDEYRYF
                     VGNGVLKLLERALPADHQGDITAVKKRFDEIYGEICLENTKPYPGITKLINTLADQGY
                     NLAVVTNKPQDHAVKIVKTLFPGCFKYIFGSSIRHPKKPDPCLTNLVINLFDVRKNEV
                     VYIGDSDVDILTAKNTKVRSIGVSWGFRGRQELLENGADLVVDHADEIKEAINDWCK"
     gene            1683..2126
                     /locus_tag="PLK68_05410"
     CDS             1683..2126
                     /locus_tag="PLK68_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HRM89884.1"
                     /translation="MIGVSSCLAGCKCTYSGDDKLIKLVKEMVMRNEAIMICPEVLGG
                     LSIPRSPCEIRDGKVVDINGVDWTKEYRLGAQKSLEILQEHDVDVVLLKAKSPSCGKN
                     YIYDGTFSHTIINGDGITCQLLEKHGIIVFNDDNIDEFLKYIERR"
     gene            2131..3066
                     /locus_tag="PLK68_05415"
     CDS             2131..3066
                     /locus_tag="PLK68_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRM89885.1"
                     /translation="MGHVLKINPVFKEMIWGGHKLRDVYGYDIPSDNTGECWAISAHK
                     NGDCTISDGEFAGKTLSSLWDEHRELFANIEGDQFPLLVKIIDACNDLSVQVHPNDEY
                     AKAHENSLGKTECWYVLNTDEGTKMVMGHHAKTKEELVKAIENDDYDNLLNKFDIKEG
                     DFFYIPSGTIHAICSGSLIYEAQQSSDITYRVYDYHRKDAQGNERQLHVQQSIDVATV
                     PYVPLAADSMVETAIENGTRTKLVSSEFFTVNKYEMTGKNTIVNDKPFQLVTVIKGNG
                     VINGNSVKMGDNFVVCADQDAVEYDGTMTVMICTL"
     gene            3066..3356
                     /locus_tag="PLK68_05420"
     CDS             3066..3356
                     /locus_tag="PLK68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89886.1"
                     /translation="MYCSNCNHEFKAASFAYKARQRCPECGTLMQIQFPSGMGFIPII
                     VAILPAVYMVSVLQFDLLVGLSVFILVYWPIEVIMNILLIYFDKYRLYEVDQ"
     gene            3461..>4561
                     /locus_tag="PLK68_05425"
     CDS             3461..>4561
                     /locus_tag="PLK68_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRM89887.1"
                     /translation="MAHLKFKFETLNAFCNEAFEKFGFTSDESKTITDVLLLSDVYGI
                     ESHGMQRLSRYHKGIEKGMIKVDAKPEIVFETPVSAVIDGHAGMGQLISKFAMEKCIE
                     KAKTVGMAIVTVRNSNHYGIAGYYAKMACDEGLMGMSFTNSEAIMVPTFARKAMLGSN
                     PIALAMPAKPYPFFFDASTTVVTRGKLEMYRKAEKELPNGWALDKDGNPSIDAPDVLD
                     NIANKIGGGIMPLGGSTEQLGSHKGYGYGMFCEIFCSILSQGLTSNHTHTNGIGGTCH
                     GFIAIDPKIFGNPEDIETHFSTFLQELRDAPKAEGQPRIYTHGEKEVEATKDRMENGI
                     DVDVKTVLEMKDLSNFVGVDFEKYFGKLDIEDN"
BASE COUNT         1580 a          593 c          902 g         1476 t
ORIGIN      
        1 aattgcaatg atggctacgg ccactatttt cagccaccaa tttttacgaa atgctgacca
       61 ctcttttttt atcatttttt ctcctttcca ttaaattata gttttatctt gaaatttaat
      121 atattaatag atataatcta tccatggata aagtttatct attaataaag catattttaa
      181 tcatctacta taagtttgtc aatcaattta taaaaaatat tttctagatt attaatctcg
      241 aataatgtta caatatatag caaaggggaa atgaagtaat gaaagtaaaa aatagtgtcg
      301 aacaagcaat ttgtttatta ataattatcg cccatagcaa tgatgaaaaa cctttaaaaa
      361 gttataatat ctctgatagt ttaggtgttt ctgattctta tttaaaaaaa attattaggc
      421 aattagttgt agctgggctg attacatctg aagcagggaa aaaaggtggt gtatgcttaa
      481 gccggaaacc tgataagatt actttgctgg atatttttga ggctatcgag ggtaaagagc
      541 cnnnnnnnnn ngtttgcaaa agcaacggga ctagtagaac gtgtttttgt taatgaatta
      601 aaagaaatca tggatcaaaa gaaggcgatg attttagatg catttaatgc tgcagaagca
      661 agttataaag aaaaattaaa aacgatcaca ttaaaaatgg ctatggtaga acgaaaaaag
      721 gattcttaag cgaatcctta aatattaacc atgctttttt tgattgtttt tatcgacaat
      781 tcgatctaag ccatatccaa ttacacgtcc aataaatgca aatacgataa accagagtgc
      841 gctccatgaa cttgtttctt tattaacaaa gccaataaca aaaataataa ctgcgatcgt
      901 taatccaaca gctgtaccta atgtcataaa tacactactt ttttctttca tcctttcact
      961 cctcgattct aaattatacc acaacaaagt gcgcatacaa gtaaaatatg tgttataata
     1021 ctgtcgtaaa taatggagtg ataagatgat aaatacaatt atatttgact tagatggtac
     1081 attgatagat tctttagttg atctttcaaa tactgttaat gttattttaa cagaaaaagg
     1141 atatcctacg catacccttg atgaatatcg ttattttgtt ggaaatggtg tattaaaatt
     1201 gcttgagcgg gctttacctg ctgatcatca gggggatatt acagctgtaa agaaacgttt
     1261 tgatgaaatt tatggtgaaa tttgtttaga aaataccaaa ccatatcctg gaatcacaaa
     1321 attaattaat actttggctg atcaaggata taatttggct gttgtgacta ataaaccgca
     1381 agaccatgca gttaaaattg ttaagacttt atttccagga tgttttaaat atattttcgg
     1441 tagtagtatt agacatccta aaaagccgga tccctgttta acaaatctag ttattaatct
     1501 ttttgatgtg cgtaaaaacg aagtcgtcta tataggcgat agtgacgttg atattttaac
     1561 tgcgaaaaat acgaaggtta gatcaatagg cgtatcatgg ggatttagag gacgtcagga
     1621 gctgcttgaa aatggtgcag atcttgttgt tgatcatgcc gatgaaatta aagaggctat
     1681 aaatgattgg tgtaagtagt tgtctggcag gatgcaaatg cacttatagt ggtgatgata
     1741 aattaattaa attagtcaaa gagatggtta tgcgaaatga agcaataatg atttgtcctg
     1801 aagtgttagg ggggttgagt attcctcgtt ctccttgtga gataagagat ggaaaagtag
     1861 ttgatattaa tggtgttgat tggactaagg aatatcgatt aggagcacaa aagagtttag
     1921 aaatattaca agaacatgat gttgatgtag ttttgttaaa ggcaaaaagt cctagttgtg
     1981 gaaaaaacta tatatatgat gggacgttta gtcataccat tataaatggt gatggtatta
     2041 cttgccaact actagaaaaa catggcataa tagtattcaa tgatgataat attgatgaat
     2101 ttttaaaata catagaaaga aggtaaaaaa atgggacacg tattaaaaat taatccagtt
     2161 tttaaagaaa tgatttgggg aggtcataaa ttaagagatg tttatggcta tgatattcct
     2221 agtgacaata ctggtgagtg ttgggcaatc tcagctcaca aaaatggaga ttgtactatt
     2281 agtgatggag aatttgctgg aaagacatta tctagtttat gggatgaaca tcgagagtta
     2341 tttgctaata ttgaaggtga ccaatttcca ttattggtaa aaattattga tgcttgtaac
     2401 gatttatctg ttcaagttca tccaaatgat gaatacgcta aagctcatga aaattcttta
     2461 ggtaaaacag aatgctggta tgttctaaat accgatgaag gaacaaaaat ggtaatggga
     2521 caccatgcta agactaaaga agagttagta aaagcgattg aaaatgatga ctacgacaat
     2581 ctgttaaata aatttgatat caaagaagga gacttcttct atattccttc aggtacgatt
     2641 catgctattt gcagtggttc attaatttat gaagcacaac aatcttcaga tattacatat
     2701 cgtgtttatg attatcatcg taaagatgct caaggaaatg aacgtcaatt gcatgtacaa
     2761 caatcgattg atgttgcaac agttccatat gttccacttg cggcggatag tatggttgaa
     2821 acagcaattg aaaatggaac gaggacgaag ttggtatcaa gtgaattttt tacagttaat
     2881 aaatacgaaa tgactggtaa aaatacgatt gttaatgata aaccattcca attagtcact
     2941 gttattaaag gtaatggtgt tattaatggg aatagtgtaa aaatgggcga taactttgta
     3001 gtttgtgctg atcaagatgc cgtagaatat gatggtacaa tgacagttat gatctgtaca
     3061 ttataatgta ttgttcgaac tgtaatcatg aatttaaagc agcttctttt gcctataaag
     3121 caagacaaag atgtcctgag tgtggaacgt tgatgcaaat ccagtttcct tcgggaatgg
     3181 ggttcattcc tatcattgtt gcaatacttc cagcagtata tatggtttca gtactgcaat
     3241 ttgatttatt ggttggatta agtgtcttta ttcttgttta ttggccaatt gaagttatta
     3301 tgaatatctt attaatatat tttgataaat accgcctata tgaagtagat cagtgatcta
     3361 ctttttttga acaataatgt aataaatcat tggcggaaat aatgactaaa tgggaacttt
     3421 gtgatagaat gttgacgata acgattaagt gaggtaaatt atggcacatt tgaaatttaa
     3481 atttgaaaca ttaaatgctt tttgtaatga agcatttgaa aaattcggtt ttacaagtga
     3541 tgaaagcaaa acaatcacag atgtattatt attgtcagac gtatatggaa ttgagtcaca
     3601 tggtatgcag cggctttcac ggtatcacaa agggattgaa aaaggcatga ttaaggttga
     3661 tgcaaaacct gagattgttt ttgaaacccc tgtttcggca gtgattgatg gtcatgctgg
     3721 aatgggccaa ttgattagta aatttgcaat ggaaaaatgt attgaaaaag ctaaaacagt
     3781 agggatggca attgtaacgg taagaaattc taatcattat ggaattgctg gttattatgc
     3841 aaaaatggct tgtgacgagg gattaatggg gatgtcattt actaactcag aagctattat
     3901 ggttcctact tttgcccgta aagcaatgct aggatctaat cctattgctt tagcaatgcc
     3961 agctaaacca tatccattct tttttgatgc ttctactaca gtagttactc gtggtaagtt
     4021 agaaatgtat cgaaaagctg aaaaagaatt accaaatgga tgggcactag ataaagatgg
     4081 taatccttct atagatgcac cagatgtttt agacaacatt gctaataaaa tcggtggtgg
     4141 gattatgcct ttaggtggtt caacggaaca attaggaagc cataaaggat atggttatgg
     4201 aatgttctgt gagattttct gttctatttt atcacaagga ctgacttcga atcatacaca
     4261 tacaaatggc attgggggaa catgtcatgg atttattgct attgatccta aaatatttgg
     4321 aaacccagaa gatattgaga cacatttttc aacgttcctg caagaattaa gggatgctcc
     4381 aaaagctgaa ggacaaccgc gtatatatac tcatggtgaa aaagaagttg aggctacaaa
     4441 agatcgcatg gaaaatggaa ttgacgttga tgtcaagaca gtattagaga tgaaggatct
     4501 atctaacttt gttggtgttg attttgaaaa atactttggt aaattagata ttgaagataa
     4561 t
//
LOCUS       DAOMTA010000152         4530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_532_length_4530_cov_2.461006, whole genome shotgun sequence.
ACCESSION   DAOMTA010000152 DAOMTA010000000
VERSION     DAOMTA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_4530_cov_2.461006"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLK68_05430"
     CDS             <1..505
                     /locus_tag="PLK68_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM89888.1"
                     /translation="KNVGIDSRKIISYLPDENYFQDWMYIKDVLSIFSDLYEDFDKEN
                     CLTLMNRFKLDKGMKIKEMSKGMKEKFQLSLVMSRKAKLYILDEPIAGVDPAAREVIL
                     DVILNNYEEDALVLISTHLISDLETIFDDVVFLKDGEIVLHQSTEDLRLERKQSIDEA
                     FREVFRC"
     gene            499..1227
                     /locus_tag="PLK68_05435"
     CDS             499..1227
                     /locus_tag="PLK68_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HRM89889.1"
                     /translation="MLKLLKYEMIQSYRQYFLTLGIFLILCVLAPLLPDFISQVLSSL
                     MIFAMLGISIAVLVNVITNFNRSMYKRPGYLTLTLPVSTEKLVGAKFIGSLIWVFVSS
                     IVLSLGILIIVFLIGNVPLNTLFDLFGELLKALGNNFGLVVINLIDSIAMVSSMILSF
                     YAIITLTKTKYIPKHKTVIGIIVYVALLILGSSLLMWQPIESFVMSLDSTASVWFSIV
                     LNLVLATAFYFFTVYLIDHKIEVE"
     assembly_gap    1309..1318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1319..1591
                     /locus_tag="PLK68_05440"
     CDS             <1319..1591
                     /locus_tag="PLK68_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM89890.1"
                     /translation="LPGSKLASVRELALEYGVNPNTIQKALSELERTGIIYSRRALGR
                     FVSEDSNLISELKKDVSLDKVKVFIEEMRKLGFSKIEVIKMIEELE"
     assembly_gap    1657..1666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1671..2117
                     /gene="spx"
                     /locus_tag="PLK68_05445"
     CDS             1671..2117
                     /gene="spx"
                     /locus_tag="PLK68_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HRM89891.1"
                     /translation="MIRIYTAPSCASCRKVKSWLKEHNIPYVEKNIFSTLLREIELKE
                     LLERSENGTDDIISKRSKIIKENDIDIDSMSISELIKFIQENPSVLKRPIMIDERRFQ
                     VGYNAEEIRVFIPRELRKLAECPSSEVCPQFISEPCSIKEAYFKDR"
     gene            complement(2167..2733)
                     /locus_tag="PLK68_05450"
     CDS             complement(2167..2733)
                     /locus_tag="PLK68_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRM89892.1"
                     /translation="MEENLEIEFKVLIDKEIYQQIYNDHHIDHQYSQTNYYLIHPKLT
                     ELKYMLRIRQKQENYELTLKQPQSYGNLETNLKIDKITKEKIITHDFVTNEIFDLLKP
                     LGLDSTMFKTDCVLTTTRCEIKTTDGLICLDQSLYNGITDYELEYEVFDYHHGKQVFL
                     DFIDQYNLKYSRNCPSKVKRLMDSLKDD"
     gene            2787..3569
                     /locus_tag="PLK68_05455"
     CDS             2787..3569
                     /locus_tag="PLK68_05455"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRM89893.1"
                     /translation="MKQYALVVKQDEMSANIAEKIKKGLTGIMEYNPDDPDLVISVGG
                     DGTMLLSVHQYMEQKVSFVGVHTGTLGFFTDYQKDEITELIAAIKADHYQMTPRHLLE
                     VDVYHKAGKETYLALNEMRIDHGYTTQVIDVYIDDELLEVFRGNGLCVSTPSGSTAYN
                     KSIGGAVIYPGSPLMQLTEVAAIQHNAYRSLGASLILDENKVIKLKGQHFNRVYLGID
                     HLSYHLDDVEKIEIRISKKVVKFIEYKEMSFIQRIRRAFISE"
     gene            3566..4402
                     /locus_tag="PLK68_05460"
     CDS             3566..4402
                     /locus_tag="PLK68_05460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM89894.1"
                     /translation="MKFIVAMKESGMLLREFLSQQELSKKAVKLIKMHGKILVNGQRQ
                     TVRYCLRYGDVVELLWPEEVSTMEPYPLALKICYEDENFLVIDKPAGLPSIPTKRYPR
                     NTLANAVVYYYQSQGIKATVHLVNRLDKDTQGLLLIAKNSYAHYLLSRDIKQVRRVYH
                     CVVEGVLTGQGTITAPIIKDKKSVKRLIHPAGKFAVTHYRGLEVSANQSKIECILETG
                     RTHQIRVHLSSINHPLVGDTLYDSIYQESYYLDSIALSFIDPFTQRLIEVKKDSKQFE
                     KN"
BASE COUNT         1541 a          534 c          857 g         1578 t
ORIGIN      
        1 gaaaaatgta ggaattgatt cacgaaaaat tatttcatat ttaccggatg agaattattt
       61 tcaagattgg atgtatatta aagatgtctt aagtattttc agtgatttat atgaagattt
      121 tgataaagaa aattgtttga ctctgatgaa tcgttttaaa ttagataagg gaatgaaaat
      181 taaagagatg tctaaaggaa tgaaagaaaa attccaacta tcattggtga tgtcacgcaa
      241 agctaaatta tatattcttg atgaaccgat agctggtgtt gatccagctg ctcgtgaagt
      301 tattctagat gttattttga ataattatga agaagatgct ctggtactta tttcaacaca
      361 tttaatcagt gatttggaaa ctatttttga tgatgtcgtg tttttaaaag atggtgaaat
      421 cgttttgcat caatcaacag aagatttaag attagaaaga aaacagtcaa ttgatgaagc
      481 ctttagggag gtatttagat gttaaaatta ttaaaatatg agatgattca atcgtatcgt
      541 caatattttt taactttagg aatatttttg attttatgtg ttttggcacc gttgttgcca
      601 gattttatta gtcaagtatt gagttcattg atgatttttg caatgttagg tatatcaatt
      661 gcagttttgg tcaatgttat tacaaatttt aatcgttcaa tgtataagcg acctggttat
      721 ttaaccttga cgttgcctgt aagtactgaa aaattggttg gggctaaatt tataggtagt
      781 ttaatttggg tgtttgttag ttcgattgtt ttatcactgg gaatattaat tattgtattt
      841 ttaattggta atgttccttt gaatacttta tttgatttat ttggtgagtt gttaaaggct
      901 ttaggtaata attttggttt agtagtaata aatttaattg attcaattgc gatggttagt
      961 tcaatgatat tatcgtttta tgctattatt acattaacaa agactaaata tattcctaaa
     1021 cacaaaactg ttattggaat tattgtttat gtagcacttt taattttagg aagcagctta
     1081 ttaatgtggc agccaattga gagctttgta atgagtttgg atagtactgc ttctgtttgg
     1141 tttagcattg ttttaaattt agtattagca acagcatttt atttcttcac tgtttattta
     1201 attgatcata aaattgaagt agagtaggtg gtagaatgaa aaatgacttc gatcctaatt
     1261 taccaattta tattcaggtc atggaagaaa taaaaaaaga gatttttgnn nnnnnnnntt
     1321 accgggaagt aagcttgctt cagtaagaga gctagcgttg gaatatggag ttaatcccaa
     1381 tacaattcaa aaagctttaa gtgaattaga aagaacggga ataatttata gcaggcgagc
     1441 tttaggacgt tttgtcagtg aagacagtaa tttgatcagt gaattaaaaa aagatgtttc
     1501 acttgataaa gttaaagttt ttattgaaga aatgaggaaa ctaggttttt caaaaataga
     1561 ggtaattaaa atgatcgagg aacttgaata atccaagttc ctcgatttgc atattatttt
     1621 actatgatta acgaaaatga tagaaaaata ataaaannnn nnnnnntatt atgattcgta
     1681 tttacacagc accgagttgt gcatcatgtc gaaaggttaa gtcttggctg aaagaacata
     1741 atattcctta tgtggaaaaa aatatttttt caaccttgct tagagaaatt gaattaaaag
     1801 aactattgga acgaagcgaa aatggaacag acgatatcat ttctaagcga agtaaaatca
     1861 ttaaggaaaa tgatattgac attgatagca tgtcaattag tgagctgatc aagtttattc
     1921 aagaaaatcc atcagtttta aaacgcccaa ttatgattga tgagcgaaga ttccaggtag
     1981 gttataatgc agaggaaatc cgggtattta ttcctcgtga gttacgtaaa ttagcagaat
     2041 gtccaagtag tgaagtttgt ccacaattta ttagtgaacc gtgctcaatt aaagaggctt
     2101 attttaaaga tagatagcag tgttatgaaa cgctgctttt ttagttctag taaaaaagat
     2161 gattatttaa tcatctttta aactatccat taaacgtttt accttgctag gacaattacg
     2221 agaatacttt aaattatatt gatcaataaa gtctaaaaat acctgtttac catgatgata
     2281 atcaaatact tcgtattcta attcataatc agtaattccg ttataaagac tttgatctag
     2341 acaaatcaag ccatcagtag tcttaatttc acaacgtgta gtagtaagta cacagtctgt
     2401 tttaaacatc gtactatcta atccaagggg ttttagaagg tcaaaaattt cattagtaac
     2461 aaaatcgtga gtaatgattt tctctttagt tattttgtca atctttagat tagtttctaa
     2521 atttccatat gattgtggtt gctttaaggt taattcataa ttctcttgtt tttgacggat
     2581 tcgcagcata tattttaatt cagttaattt agggtgaatc aaatagtaat tagtttgact
     2641 atattgatgg tcgatgtggt gatcgttata gatttgctgg tatatttctt tgtcgatcaa
     2701 tactttaaat tctatttcta agttttcttc catagtagcc tccattagta tatactatag
     2761 tatatacgct aaggttggtg aaaacaatga aacaatatgc attagtagta aaacaggatg
     2821 aaatgtctgc taatattgct gaaaaaatta agaaagggtt aaccggaatt atggaatata
     2881 atcccgatga tcctgatttg gttattagtg ttggtggtga tggaacaatg cttttgtcag
     2941 tgcatcaata tatggagcaa aaagttagtt ttgtaggtgt tcatacggga acccttggtt
     3001 tttttacaga ttatcaaaaa gatgaaatta ctgaattgat tgcggctata aaagctgatc
     3061 attatcaaat gacgccccga catttattag aagttgatgt gtatcataaa gcaggtaaag
     3121 aaacatattt agcattaaat gagatgcgca ttgatcatgg gtatacaact caagtaattg
     3181 atgtctatat tgatgatgag cttttggaag tatttagagg aaatggtctt tgtgtttcta
     3241 caccatcagg atcgacggct tataataagt caattggtgg agctgttatt tatccaggaa
     3301 gtcccttaat gcaactgaca gaagtcgccg ctatacaaca taatgcttat cgaagtttgg
     3361 gtgctagttt aattttagat gaaaataagg tgattaagtt aaaaggacag cattttaatc
     3421 gagtttattt gggaattgat cacttatctt atcatcttga tgatgttgaa aaaattgaaa
     3481 ttagaatttc taagaaagta gtaaagttta tagaatataa ggaaatgtct tttattcaac
     3541 gaattagaag ggcttttatc agcgaatgaa atttattgtc gcaatgaaag aaagcggaat
     3601 gttgttaaga gaatttctta gtcaacagga gcttagtaaa aaagctgtta aattaattaa
     3661 aatgcatggg aagattcttg ttaatggtca gcgtcaaacg gtacgatatt gtttgcggta
     3721 cggtgatgtt gttgaattat tgtggcctga agaagttagt actatggaac catatccgct
     3781 agctttaaaa atttgctatg aagatgaaaa ttttttagta attgataagc cggcaggatt
     3841 accaagtatt cctacaaagc gttatcctcg aaatacttta gccaatgcag ttgtttatta
     3901 ctatcaaagc cagggaataa aggcaactgt tcatttggtt aatcgattgg ataaagatac
     3961 ccaaggatta ttgttgatag ctaaaaacag ttatgctcat tatttattgt ctagagatat
     4021 caagcaggta cgccgtgtct atcactgtgt agtagaggga gtattgacgg gacaaggaac
     4081 gattacagcg ccaattatta aagataaaaa aagtgttaaa agactgattc atccagctgg
     4141 gaaatttgct gtaacccatt atcggggact ggaggtaagt gctaatcaga gtaaaataga
     4201 gtgtatctta gaaacaggaa gaactcatca aattagggtt cacctatcta gtatcaatca
     4261 tccgttagtt ggggatacac tttatgattc gatttatcaa gaatcttact atttggatag
     4321 tatagcatta agttttattg acccttttac acaacgtctg attgaagtaa agaaagattc
     4381 aaaacaattt gaaaagaatt aaaatgattc ctcataaatg gatagcaaat agctgaaatt
     4441 aaatgaagga atctttttta tcgaaataaa aagatgattt atcatttaat aaatcatctt
     4501 atataattaa taagttaata gcgctttttt
//
LOCUS       DAOMTA010000153         4529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_533_length_4529_cov_2.234689, whole genome shotgun sequence.
ACCESSION   DAOMTA010000153 DAOMTA010000000
VERSION     DAOMTA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_533_length_4529_cov_2.234689"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLK68_05465"
     CDS             complement(<1..670)
                     /locus_tag="PLK68_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89895.1"
                     /translation="MADQSTDLIKGSIYKSILWFSIPLLIGNLFQQLYNTVDSYVVGN
                     YVNSNALAAVGASTPVINMLVGFFMGLATGAGVVISQYFGARRIEKMQRAVHSSLALT
                     GVLCVVFTVVGLLCTQPLLKAIGVPQEVLPHSSMYLMIYFCGISFGLVYNMGSGILRA
                     IGDSKRPLIYLIVASVVNIALDFLFVCSFNWGIAGVGIATVIAQAISAVMVMYQLIHT
                     KEDYR"
     gene            812..1663
                     /locus_tag="PLK68_05470"
     CDS             812..1663
                     /locus_tag="PLK68_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM89896.1"
                     /translation="MEKKIIFFDVDGTLVSDTGGIEHVPESAKRAIALTRAKGNLVYL
                     CTGRSKAEIYDFILECGIDGVIGAGGGYIEIGNQMLYHKKVTNKAVNHMVDYFDTNNF
                     DYYVESNGGLFASKNLVKRLERIIYGDYENDPQAKARYEKKDSHFINALIEGQEMRRD
                     DINKACFLENPNIPFNNIIKEFDREFNVIHCTVPAFGDNSGELSVPNIHKANAIETLI
                     NYLGIERKNTYAFGDGMNDKEMLEYVNIGIAVGNAKEGLKAVADEITDNIDNNGIYNS
                     MKKHNLI"
     gene            complement(2064..2816)
                     /locus_tag="PLK68_05475"
     CDS             complement(2064..2816)
                     /locus_tag="PLK68_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRM89897.1"
                     /translation="MNAVKIINYLESIFPLNLQMSWDKCGIQVGDCNQQVTSIMVALN
                     ADIESLQKAIDQNCQMLVTHHPFLLEEIMNLDFHNHHGKFIEMAIKNNILVYSLHTCL
                     DRGKDGISMNDWLINALGVHDVSCYDEFQVGKMALLNQPCMTSELVKKVKDTFNVPVR
                     LAGKEVEIKTIAICGGSGADDLEQLAGRVDAYITGDSKHRHAKYALDHDIVLIDVPHH
                     LEVIMEKRLASLLSNLGITVKEANSQDYYSYY"
     assembly_gap    2918..2927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2928..3488)
                     /locus_tag="PLK68_05480"
     CDS             complement(<2928..3488)
                     /locus_tag="PLK68_05480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM89898.1"
                     /translation="MKLSKRLQLIADVISKYKQGSVLADIGSDHGYLPCYLVKNKIIT
                     CAYACDVAQGPLDSAKETIKQYGLEDKVFALLGNGLNPILDREVDMISIAGMGSYLIS
                     EILEEHREYLRNVKLMFLQANANNDHLRKYLFANDWIIIDEQMVKDAGHIYEVMVVTA
                     RQNKAITYNRKDEEFGPILINNQTPLF"
     gene            complement(3490..>4529)
                     /gene="rpoD"
                     /locus_tag="PLK68_05485"
     CDS             complement(3490..>4529)
                     /gene="rpoD"
                     /locus_tag="PLK68_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRM89899.1"
                     /translation="VEDEVLLAGVPAELDDDIGLDDDLGLDGDTPDLDFAGDFDMMTG
                     DTIHMYQVADADAELDQNQLGSNVKINDPVKMYLKEIGRVELLTHDEEIDLAKKILEG
                     DEDAKKELAAANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDYTKGFKFST
                     YATWWIRQAITRAIADQARTIRIPVHMVETINKLTRIQRQLIQELGREPSAEEIAEKM
                     DGMTPEKVREIQKISLEPVSLETPIGEEDDSHLGDFIEDEGAMSPDDYAANELLKDEL
                     NEVLLELTDREEKVLRLRFGLDDGRTRTLEEVGKEFSVTRERIRQIEAKALRKLKHPS
                     RSKRLKDFLDR"
BASE COUNT         1532 a          865 c          624 g         1498 t
ORIGIN      
        1 ctcgataatc ttcttttgtg tgaatcaact ggtacattac cattaccgca ctaattgcct
       61 gagcaattac tgttgcgatt ccaactccag cgattcccca attaaaacta caaacaaata
      121 aaaaatctaa agcgatgtta acaacactgg caacgatcaa ataaattaaa ggtcgttttg
      181 aatcaccgat tgctcttaaa attcctgaac ccatattata aacaaggcca aaagaaatac
      241 cacaaaaata aatcatcaaa tacatcgatg aatgaggtaa aacttcttgt ggtactccaa
      301 tagcctttaa aagcggttgg gtacacaata gccctacaac cgtaaaaaca acacataaaa
      361 ctcctgtaag agccagtgat gaatggacag cacgctgcat tttttcaatc cgtcgtgccc
      421 caaaatattg ggaaattaca actccagccc ccgttgccag tcccataaaa aagcctacaa
      481 gcatattaat tactggggta gaagctccta cagcagctaa tgcattactg ttaacatagt
      541 tgccaactac ataagaatcg actgtgttgt ataattgctg aaataaatta ccgatcaata
      601 atggaattga aaaccataaa attgatttat aaatactccc cttaattaaa tccgtacttt
      661 gatctgccat aaatctccct cgctttaaca tgaaaatgat acaccttatt aaccagcatt
      721 acaagaattg cttaaacaat tcttgttcaa aatatgaaaa tcaattatat ctaaatatca
      781 cgatgttata atttatacaa ggagaaatta tatggaaaaa aagattatat tttttgatgt
      841 tgatggcaca ctcgtaagtg ataccggcgg gattgaacat gtacctgaat ctgccaaaag
      901 agcaattgct ttaactagag ctaaaggaaa tttagtatac ttatgtactg ggcgcagcaa
      961 ggctgaaatc tatgatttca ttcttgaatg tggaattgac ggagtaattg gagctggcgg
     1021 tggctatatt gaaataggaa atcaaatgct ctatcataaa aaagtgacaa ataaagcagt
     1081 taatcatatg gttgactatt ttgatactaa taattttgat tactatgttg aaagtaatgg
     1141 cggtttattt gcaagcaaaa atctagttaa aagacttgaa agaattatat atggtgatta
     1201 tgaaaatgat cctcaggcca aagctcgcta tgaaaaaaaa gacagccatt ttattaacgc
     1261 tctaattgaa ggtcaagaga tgcgtcgtga tgacattaat aaagcctgct ttttagaaaa
     1321 tccgaatatt ccatttaata atattattaa agaatttgat cgagaattca atgtaatcca
     1381 ttgtactgtt cctgcttttg gtgataacag tggcgaatta tcagtcccaa acatccacaa
     1441 agctaatgct attgaaacat tgatcaacta cctaggaatt gaacgtaaaa atacttatgc
     1501 atttggtgat ggaatgaatg acaaagaaat gcttgaatat gttaatattg gaatcgccgt
     1561 cggcaatgct aaagaagggt taaaagcagt tgctgatgaa ataactgaca atatagataa
     1621 taacggtatt tacaattcta tgaaaaagca taatttgatt taaacatata attgaaatat
     1681 aatattatta attaagactg tattgccgta aaggaatcga atcattgggt tcctttcttt
     1741 ttttatagtt gattgatttt tagaacataa aataagagta aaatttgaac atattgtaca
     1801 aatttctaat tcatataatc gatatcatca cttaagtgcc taactaggtt ccctaagacc
     1861 atatcctaat aaaattatcc tcaatatcaa tattttaaac agttatttta atacaactaa
     1921 aaacacacat cccattatat gcttacatca atagctccaa ttatgttata gcataaaagt
     1981 caaaaaccaa gatacctact ttatgtaaat atcttggcta taattactta cattcaactt
     2041 tatgacatgc atcaaaatac tttttaataa tatgaataat aatcctgact atttgcttct
     2101 tttacagtaa tcccaagatt actcaataaa ctagcaagtc gcttttccat aattacttca
     2161 agatgatgag gaacatcgat taagacaata tcatgatcca atgcatattt agcatgacga
     2221 tgtttactat cacccgttat atatgcatcc actcgaccag ctagctgttc aagatcatcg
     2281 gccccactac caccacaaat agcaattgtt ttaatctcta cttctttacc agcaagacgt
     2341 actggtacat taaaagtatc tttaactttc ttaactaact cgctagtcat acaaggctga
     2401 tttaaaagtg ccattttccc cacttgaaat tcatcataac agctaacatc atgaacacct
     2461 aaagcattaa taagccaatc attcatcgaa ataccatctt tgcctcgatc aagacaagta
     2521 tgtaggctat aaacaagaat attgtttttt attgccattt caataaactt tccatgatga
     2581 ttatggaaat caagattcat tatttcttct agcaaaaatg gatggtgtgt aactaacatt
     2641 tgacaatttt gatcaattgc cttttgaaga ctttctatat cagcatttaa tgcaaccatt
     2701 atacttgtta cttgttgatt acagtcacct acttgaattc cacatttatc ccacgacatt
     2761 tgtaagttaa gtggaaagat tgattccaaa taattaatta ttttaacggc attcatggag
     2821 cacctcttca atcattttca ttttatcagc aatttcatga taacgcggat gatcatgtgg
     2881 taatgtattt tgaatctttt cataaacttg atactgtnnn nnnnnnnaaa caatggagtt
     2941 tgattgttga ttaaaattgg tccaaactct tcatccttac gattatatgt aattgcttta
     3001 ttctgtctcg ctgtaactac catcacctca tatatatgcc cagcatcctt aaccatctgt
     3061 tcatcaatga tgatccaatc attagcaaat aaatatttac gtaaatgatc attattggca
     3121 ttagcttgta aaaacatcag tttaacattt cttaaatact cacgatgctc ttcaagaatc
     3181 tcactaatta aataagatcc cattccagca atactgatca tatccacttc acgatcaaga
     3241 attgggttca gcccgtttcc caatagtgca aaaactttat cttctaatcc atattgtttg
     3301 attgtctctt ttgcggaatc taatggtccc tgtgctacat cacaagcgta agcacaagtt
     3361 ataatcttat ttttaactaa gtagcatggc aaatagccat gatcacttcc aatatctgct
     3421 aaaacacttc cttgtttata tttactaatt acatcagcaa ttagctgtaa tctttttgat
     3481 agtttcattt taacgatcta agaagtcttt taatcttttt gaacgtgaag gatgttttaa
     3541 tttacgaagt gccttagctt cgatttgacg aattctttcc cgggttacac taaattcttt
     3601 ccctacttct tcaagcgttc tagttcttcc atcatctaaa ccaaatctta agcgtaatac
     3661 tttttcttca cgatcagtta attctaataa gacttcattc aattcatctt ttagaagttc
     3721 attagcagca taatcatctg gcgacattgc accttcatct tcgataaaat ctcctaaatg
     3781 agaatcatct tcttcaccta ttggtgtttc caaagatact ggttctaaag aaattttttg
     3841 tatttcacga actttttctg gtgtcattcc atccattttt tcagcaattt cttcggcact
     3901 aggctcacgc cctaattctt gaatcaattg acgctgaatt cttgtcaatt tattaattgt
     3961 ttctaccata tgtacaggaa tacgaattgt tctagcttgg tcagcaatag ctcttgtaat
     4021 agcttgacga atccaccatg ttgcataagt tgaaaattta aatcccttag tataatcaaa
     4081 tttctcgact gctttgatta aacccatgtt accttcttga atcaaatcta aaaataacat
     4141 tcctcttcca acatatcttt tagcaatcga tacaactaaa cgtaagttag cagcagcgag
     4201 ttcctttttt gcatcttcat ctccttctaa aatcttttta gcaaggtcta tctcctcatc
     4261 atgagtaagc agttcaacgc gtccaatctc ttttaaatac attttaactg gatcattgat
     4321 cttgacatta cttcctaatt gattttgatc taattccgca tcagcatcag caacttgata
     4381 catatgaatc gtatcacctg tcatcatatc gaaatcacca gcaaaatcta aatctggagt
     4441 atcaccatct aatcctaaat catcatctag tcctatatca tcatctaatt cagcaggtac
     4501 accagctaat aatacttcgt cttctacat
//
LOCUS       DAOMTA010000154         4526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_534_length_4526_cov_1.983002, whole genome shotgun sequence.
ACCESSION   DAOMTA010000154 DAOMTA010000000
VERSION     DAOMTA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_534_length_4526_cov_1.983002"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLK68_05490"
     CDS             <1..339
                     /locus_tag="PLK68_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89900.1"
                     /translation="RYEIPLFIVENGLGYDDQVDENGYVEDDYRITYLREHIKEMKSA
                     ILEDGVDVIGYTPWGCIDLVSAGTGEMKKRYGFIYVDRDDQGNGTLKRSKKKSFAWYK
                     KVIATDGEDL"
     gene            336..1220
                     /locus_tag="PLK68_05495"
     CDS             336..1220
                     /locus_tag="PLK68_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89901.1"
                     /translation="MNIAVFDVGGTFIKHCLMIDGKITQAGKISTPQDSQESFLKAIK
                     AVLDKMSNIEGIAFSLPGVIDVYRQYIYAGGSLRYNDNCDLKEWENYFNLPIQAENDA
                     RCAAIAELEQGNMQGIQNGLVLTFGTGVGGGIIINGDIFKGSHLISGEVSMIFAHRIS
                     DVTNHHLFGALGSIKNLIDKIAAAKGVKTDDGRLIFDWIKTGDLISCELFDDYCDDVI
                     WQLHNIQCILDPQRICIGGGVSENQIFIDGIKTAVKRFYQSLPIPFPQPEIVKCKYCN
                     DANMVGAYLHYLRKNDER"
     gene            complement(1232..2425)
                     /locus_tag="PLK68_05500"
     CDS             complement(1232..2425)
                     /locus_tag="PLK68_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89902.1"
                     /translation="MIQTKQELINLLPTIYQNLNTPLFLISPDLKIIASPKKFLKLEI
                     NYFQQIFNFTIMKQHEIYIHFHQNATYFFFCTKLDEIPYICVGPIFNRKITTQDSPAE
                     YELFQHVISSYTLDDFFNLPSITVETKNHFIFIYQIITGKILDSKLLKITFKGSKDNP
                     LKQENSLEKEIFQIRETPLHEFSYTYEQKILNYIQNEDSTSARILMIELLQIKDERHL
                     SKNQLQSAKYKVVAAIAVFTRGVISIGVPVDKAYSLSDVYIVKVDQSNTINQLHKLIS
                     DAIIDFTQLVKRYRNIQNPYWVKICKNYISHNLHKNITLLDLAKVTEMNTTYLSTQFK
                     KTTGQSIKQYINHKKIQEAQFLIKNSQYSLAQIADILQFSSQSHFNKVFKQIVGKSPI
                     QYKNS"
     gene            2502..>3465
                     /locus_tag="PLK68_05505"
     CDS             2502..>3465
                     /locus_tag="PLK68_05505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM89903.1"
                     /translation="MFKNNFLWGGATAANQFEGGYNLGGRGLATSDTKTNGSMNKKRK
                     HSFLDSNGKIVYLHGSEKIPQAYQSVIDEKMYYPSHRAVDFYHRYEDDIALLAQMGFK
                     CFRMSISWTRIYPRGNESSPNQAGLDFYDHIFDECLKYGIEPIVTLNHFDMPIYLADH
                     QNGWLNRQTVEYFIQYCKTVFKYYRGKVKYWMTFNEINLLRGYDTLGVHEMTPARYYQ
                     ALHHIFVASAKAVILGHKIDSNNQIGMMLANILTYSETCNPRDVALELDVSRKLKYFY
                     SDVQCRGYYPSYIVNSLKQQGITINSLEDDDNLLKNGCVDYIGFS"
     assembly_gap    3466..3475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3476..3964
                     /locus_tag="PLK68_05510"
     CDS             <3476..3964
                     /locus_tag="PLK68_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89904.1"
                     /translation="SGVVTTRKDAEMTLGNGIKMAANPYLKESEWKWPIDPIGLRISL
                     NLLWDRYQKPLFIVENGLGANDQIAEDGKIHDEYRIDYLREHIIEMKKAVDEDGVELI
                     GYTPWGCIDLVSAGTGEMKKRYGFIYVDMDDNGKGTLKRSCKESFYWYQKVIKSNGES
                     LS"
     gene            4022..>4526
                     /locus_tag="PLK68_05515"
     CDS             4022..>4526
                     /locus_tag="PLK68_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM89905.1"
                     /translation="MLLKQMKYFISVVECNSFTEAAEQCYISQSAISQQIKALEQELG
                     VDLIKRNNRQFTLTPAGEYFYRHGVELVSEIDNLKRETVRRGQDQELSLKIGYLRCYG
                     AQELHHAIAKFSKTYSEVSLSIVNGTHEELYDLLRSGQVDLVISDQRRAFNDDYYNYE
                     LLYSDCYI"
BASE COUNT         1556 a          515 c          886 g         1559 t
ORIGIN      
        1 cgttatgaaa tcccgttatt tattgttgaa aatggcctcg gatatgatga ccaggtggat
       61 gagaatggct atgttgaaga tgattatcgg attacttatt taagagaaca tattaaagaa
      121 atgaaaagtg ctattcttga agatggtgtt gacgtaattg gctatacacc ctggggatgt
      181 attgatcttg tctctgcagg gactggagaa atgaaaaaac gttatggttt tatctatgtt
      241 gatcgtgatg atcagggaaa tggaacatta aaacgtagta agaaaaaatc atttgcctgg
      301 tataagaaag ttattgctac tgatggagaa gatttatgaa tatagcagtt tttgatgtcg
      361 gtggtacatt tattaaacat tgcctgatga ttgatggtaa aataacgcaa gctggcaaaa
      421 tatcaactcc tcaagatagt caagaaagtt tcttaaaagc aattaaagca gttttagata
      481 aaatgagcaa tattgaagga attgcctttt cgttgccagg ggtcatcgat gtttatcggc
      541 agtatattta tgcgggtggg agtctaagat ataacgataa ctgtgatttg aaagaatggg
      601 aaaattattt taatttgcct attcaggctg aaaacgatgc tcggtgtgca gccattgcag
      661 aacttgaaca aggaaatatg caaggcattc aaaatggttt agtacttact tttggaactg
      721 gggtaggtgg tggaattatt attaatggtg atatttttaa aggaagtcat ttgattagtg
      781 gcgaagttag tatgattttt gcacatagaa tcagtgacgt aactaatcat catctttttg
      841 gagcattggg tagtattaaa aacctaattg ataaaatagc agctgctaaa ggtgttaaaa
      901 cggatgatgg cagattgatc tttgattgga tcaaaacagg tgatttgatt agctgtgagc
      961 tttttgatga ctattgtgat gatgtgattt ggcagcttca taatattcaa tgtatcctag
     1021 atccacaacg catttgtata gggggtggag ttagtgaaaa tcaaattttt attgatggaa
     1081 ttaagacggc agtaaaacga ttttatcaat cattgcctat tccatttcct cagccagaga
     1141 tcgttaagtg caagtactgt aatgatgcta atatggtagg cgcttatctt cactatttaa
     1201 gaaaaaacga tgagagataa actttcatcg tttaactatt tttatattga atagggcttt
     1261 ttccaacaat ttgtttaaaa actttattaa agtgactttg acttgagaat tgtaaaatat
     1321 cagcaatttg agctaaagag tattgagagt ttttaattaa aaattgagct tcttggattt
     1381 ttttatgatt aatgtattgt ttaattgatt gaccggttgt ttttttgaat tgggttgata
     1441 agtaagtagt attcatctct gttaccttgg ctaaatctaa taaagtaata ttcttatgta
     1501 aattatgaga aatatagttt ttgcaaattt ttacccaata gggattttga atatttcgat
     1561 aacgtttaac taattgtgta aaatcaatga ttgcgtcaga aataagtttg tgaagttgat
     1621 taatagtatt agactggtcg actttaacaa tatacacatc acttaacgaa taagctttat
     1681 cgacaggaac accgatactg attactccac gagtaaatac cgcgatagct gcaacaactt
     1741 tatatttagc tgactgaagc tgatttttag ataaatgacg ttcatctttg atttgaagca
     1801 gttcaatcat taagattcta gcactggtac tatcctcatt ttgaatataa tttagaattt
     1861 tttgttcata agtataacta aattcatgca aaggtgtttc gcgaatttga aagatttctt
     1921 tttcaagaga attttcttgt tttaaaggat tatccttaga acctttaaaa gtaattttta
     1981 gtaatttaga atcaagaatt ttaccagtaa ttatttgata aataaaaata aaatgatttt
     2041 ttgtttctac agtaatgctg gggagattaa agaaatcatc tagcgtatac gatgagatta
     2101 catgttgaaa taactcgtac tcagcaggtg aatcttgggt tgttattttt cgattaaaaa
     2161 taggtcctac acaaatataa ggaatttcat ctagcttagt acaaaaaaag aaatatgtgg
     2221 cattttgatg aaagtgaata tatatttcat gctgtttcat aattgtaaaa ttgaaaattt
     2281 gttgaaaata atttatttct aattttaaaa atttttttgg agaagcaatg atctttaaat
     2341 caggagaaat taaaaatagt ggagtattta aattttgata aatagtggga agtagattga
     2401 ttaattcttg tttcgtttga atcataaaat catcctttca atattattgt ataatgattt
     2461 gtttttagaa gatataaaat tttaagaaaa gaggataaaa aatgtttaaa aataattttt
     2521 tatggggtgg agctactgct gctaaccagt ttgaaggtgg ctataattta ggaggacgtg
     2581 gtcttgcgac atcggatact aaaacaaatg ggtcaatgaa taaaaaacga aaacatagtt
     2641 ttttagattc taatggtaag attgtttatt tacatggtag tgaaaaaatt ccccaagctt
     2701 atcaaagtgt tattgatgaa aaaatgtatt atccaagtca tagagcagtg gatttttatc
     2761 atcgttatga agatgatatt gctttacttg ctcaaatggg ttttaagtgt tttagaatgt
     2821 caatatcatg gacacgtatt tatcctaggg gaaatgaaag ctcacctaat caagcaggtc
     2881 tagatttcta tgatcatatt tttgacgaat gtttgaaata tggaatagaa ccaattgtta
     2941 ctcttaatca ttttgatatg ccaatttatt tggctgatca tcaaaatggc tggttaaatc
     3001 ggcaaactgt tgaatatttt atacaatatt gcaaaactgt ttttaaatat tatcggggca
     3061 aagtaaaata ttggatgact tttaatgaaa ttaatttgtt aaggggatat gatacattgg
     3121 gagtacatga aatgaccccc gctcgctact atcaagcctt acatcatatt ttcgttgcta
     3181 gtgcaaaagc agttatttta ggacataaaa tagattcaaa taatcaaatt gggatgatgt
     3241 tggcaaatat tcttacatat agtgaaactt gcaatcctag agatgttgct ttagaattag
     3301 atgtttcgcg taaattaaaa tatttttaca gtgatgttca atgtcgagga tattatcctt
     3361 cttatattgt aaattcttta aaacagcaag ggataacgat taacagttta gaagatgatg
     3421 ataacctttt aaaaaatggt tgtgttgatt atattggttt tagctnnnnn nnnnntctgg
     3481 agtagtaaca actcgcaagg atgccgaaat gacattaggc aatggtatta aaatggcagc
     3541 aaatccttac ttaaaagaaa gtgagtggaa atggccaatt gacccgatag gattaagaat
     3601 ttccttaaat ttattatggg atcgttatca aaaaccatta tttatcgttg aaaatggttt
     3661 aggtgcaaat gatcaaattg ctgaagatgg aaaaatccat gatgaatatc gcattgatta
     3721 tttaagagaa catattattg aaatgaaaaa agctgtagat gaagatggag tagaattaat
     3781 tgggtataca ccatggggat gtattgattt agtttcagcg ggaactggag aaatgaaaaa
     3841 acgatatggt tttatttatg ttgatatgga tgataatggt aaaggaacat taaaaagaag
     3901 ctgcaaagag tcattttatt ggtatcaaaa agtaattaaa agtaatggcg aaagcttaag
     3961 ctagctttaa ttgtaaaaaa tatgatataa tttttataca agttttttgg gaggataata
     4021 tatgttgttg aaacaaatga aatattttat tagtgtagtc gagtgtaata gttttacaga
     4081 agcagctgaa caatgttata tatctcaatc tgcaatttcc caacaaatta aagcattaga
     4141 gcaggaatta ggtgttgatt tgatcaaacg taataatcga caatttacct tgactcctgc
     4201 tggtgagtat ttttatcgtc atggagtgga gctggtaagc gaaatagata atttaaagag
     4261 agaaactgta cgtcgcggac aagatcagga gttatcttta aagataggat atttaagatg
     4321 ttatggagct caagaattac atcatgcaat cgctaaattt agcaaaacat attctgaggt
     4381 atcactatca attgttaatg gaactcatga ggagttatat gatcttttac gctctggaca
     4441 agttgatttg gtaatcagtg atcaacgacg agcatttaat gatgattatt acaattatga
     4501 gttattgtat agtgattgtt atattg
//
LOCUS       DAOMTA010000155         4516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_535_length_4516_cov_2.891504, whole genome shotgun sequence.
ACCESSION   DAOMTA010000155 DAOMTA010000000
VERSION     DAOMTA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_535_length_4516_cov_2.891504"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..3700
                     /gene="rpoB"
                     /locus_tag="PLK68_05520"
     CDS             29..3700
                     /gene="rpoB"
                     /locus_tag="PLK68_05520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM89906.1"
                     /translation="MENKITEAKSKITRRNYSRISGSLELPNLVEIQTNSYKWFKEVG
                     IKEVFEDIYPITNFNETLSLEFVDCTFDEAKYSVEESKDRDANYAAPIRASLRLVNSG
                     TGEIKEQEVFMGDFPLMTDSGTFIINGAERVIVSQLVRSPGAYFADALDKSGKTVFNG
                     SIIPSRGTWLEFETDAKDVLNVRIDRNRKMPGTVLLRALGLSSNDEIIDVFGEHEYII
                     NTLAKDNTTNTEEALIEIYNKLRPGEPATLEGANNLLYTRFFDCKRYDLAKAGRFKFR
                     KKLSLLDRIAGRILAEDLVDVDGNVVYPEGTVVTQEVSDTIKPILEAGAHTRELDTNP
                     RLESNGVIQVLDVYVDDTKTKKMRVIGTDLSLDSKFVTISDMMAAYSYMFNLVDIYDA
                     LDLTAEDRVNLMARIGLLDDIDHLGNRRVRSVGELIQNQFRIGLSRMERVVKERMSLS
                     EVDSITPQSLTNIRPLTAAIKEFFSSSQLSQFMDQINPLAELTNKRRLSALGPGGLSR
                     DRAGYEVRDVHASHYGRICPIETPEGPNIGLISTLASYAKINQYGFIETPYRKVNNCV
                     IDEHDVRYLTADEEKNYIIAQANVRTDKDGTILDAQVIARHLGENIMAKREEVDYIDI
                     SPKQIVSVATSCIPFLENDDATRALMGANMQRQAVPLLNPHTPFVGTGMEHQAARDSG
                     AAVVSREDGIVTYVDAKKVIVEDNEGVEHRYRLSKFKISNNGTCINHKPIVKTGEKVL
                     KGQVLADGPAMEQGELALGQNVLVGFMEWNGYNYEDAVIMSERLVKDDVYTSVHIDEY
                     AIECRDTKLGPEEITRDIPNVGDEARKNLNSDGIIMIGAEVKEGDILVGKVTPKGQAE
                     LSAEEKLLLAIFGEKSREVKDNSLRVPHGGAGIVHDIKVFERKNGDELQPGVNKVVKV
                     YIVQKRKISEGDKMAGRHGNKGVISKILPIEDMPHLEDGTPLDIMLNPLGVPSRMNIG
                     QVLELHLGYAARQLGLYIATPAFDGLHPSDLEDIMAEAGMSKDGKQPVISGRTGEYFD
                     NNISVGIMYMIKLAHMVDDKLHARSVGPYSLVTQQPLGGKAQNGGQRFGEMEVWALEA
                     YGAAYTLREILTVKSDDVVGRVKTYEAIVKGQPLPEPGLPESFRVLKKELQALALDIR
                     LLDENDNEVDMRNIEEEEHRFPRSIDKDEVIETPETEDELEEEILEDDLDVEEEEDFE
                     DIDEDFDEELEEIEESESL"
     gene            3712..>4516
                     /locus_tag="PLK68_05525"
     CDS             3712..>4516
                     /locus_tag="PLK68_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRM89907.1"
                     /translation="MANTNKFSAIQIGLASPEKIREWSYGEVKKPETINYRSQKPEKD
                     GLFCEKIFGPSKDWECSCGKYKKVRYKGVVCDRCGVEVTKSAVRRERMGHIELATPIA
                     HIWYLKGIPSRMGLILDMSPKQLEEIIYFVSYVVIDKGSTPLEYKQVLSERDYRKCFE
                     QFGHTFEAQIGAEAIQTLLQQVDLDAEFAKVTKELKEAQGQKRTKLLKRLEAIEAFRT
                     STNEPEWMILEALPVIPPDLRPMLQLDGGRFATSDLNDLYRRVITRNNRL"
BASE COUNT         1549 a          640 c          948 g         1379 t
ORIGIN      
        1 gagaaatata aaggggtgga gttaatacgt ggaaaacaaa ataactgaag caaaaagcaa
       61 gattactcgt cgtaattatt caagaattag tggatcttta gaacttccaa atttagtaga
      121 aattcaaaca aattcatata aatggtttaa agaagtaggt attaaggaag tatttgaaga
      181 tatctaccca attactaact ttaacgaaac attgtcgtta gaattcgtag attgtacatt
      241 tgatgaagca aaatattcag tcgaagagag caaagaccgt gatgctaact atgctgcacc
      301 aattagagca tctttacgtt tagttaattc tggtactggt gaaattaaag aacaagaagt
      361 atttatggga gatttcccat tgatgactga ctcaggaact ttcatcatta acggtgctga
      421 acgtgttatc gtatcacaat tagttcggtc tccaggggca tattttgctg atgccttaga
      481 taaaagtggt aaaacagtat ttaatgggag tattatccct tcaagaggta cttggctaga
      541 atttgaaaca gatgctaagg atgttttaaa cgtgcgtatc gatcgtaata gaaagatgcc
      601 gggaactgta ttattaagag ctttaggtct ttcaagtaac gatgaaatca tcgatgtttt
      661 tggtgaacac gagtatatca tcaacacttt agctaaagat aatacaacta atactgaaga
      721 agctttaatt gaaatttata ataaattaag accaggtgaa ccagcaacat tagaaggtgc
      781 taacaactta ttatatacgc gtttctttga ttgtaaacgt tatgatctag ctaaagctgg
      841 acgctttaag ttcagaaaaa aattgagcct tttagatcgt attgcgggac gtattttagc
      901 agaagattta gttgatgttg acggaaatgt agtataccca gagggtactg tagttactca
      961 agaagttagt gatacaatta aaccaattct tgaagctgga gctcatacac gtgaacttga
     1021 taccaaccct agattagaat caaacggtgt gatccaagta ttagatgttt atgtagatga
     1081 tactaaaact aaaaagatgc gagttattgg tactgattta tctttagata gtaagtttgt
     1141 tactatttct gatatgatgg ctgcttattc atatatgttt aacttagtag atatctatga
     1201 tgcgttagat ttaacagctg aagatcgtgt taacttaatg gctcggattg gattattaga
     1261 tgatattgac catttaggta atcgtcgtgt tcgttctgtt ggtgaattaa ttcaaaatca
     1321 atttagaatt ggtttatcaa gaatggaacg ggttgtaaaa gaaagaatgt cattgtctga
     1381 agttgattca attacaccac aatcacttac taatattcgt ccgttaactg cggcaattaa
     1441 ggaatttttc tcttcttcac aattgtctca atttatggat caaatcaatc cccttgccga
     1501 acttactaat aaacgtcgtt tatcagcatt agggcctggt ggtctaagcc gtgatcgtgc
     1561 tggttatgaa gtccgtgacg tacatgcgtc tcactacgga agaatttgtc caattgaaac
     1621 acctgaaggt ccaaacatcg ggttgatttc aacattagct tcttatgcta aaattaatca
     1681 atatgggttt attgaaacgc catatcgtaa agtaaataat tgtgttattg atgaacatga
     1741 tgttcgttat ttaactgctg atgaagaaaa aaattatatt attgcgcaag caaatgttag
     1801 aactgataaa gatggtacga ttctagatgc ccaagttatt gctcgtcact taggagaaaa
     1861 tatcatggct aagcgcgaag aagtagacta tatcgatatt tcaccaaaac aaattgtatc
     1921 ggttgctact tcttgtattc cgttccttga aaatgatgat gctactcgtg ctttaatggg
     1981 tgccaacatg caacgtcaag ctgtaccatt gttaaatcca catacgccat ttgttggaac
     2041 tgggatggag catcaggcag ctcgtgattc aggtgcggca gtagtatcac gtgaagatgg
     2101 aattgtaaca tatgttgatg ctaaaaaagt aattgttgaa gataatgaag gggttgaaca
     2161 tcgctaccgt ttatctaagt ttaagatttc aaacaacggt acatgtatta accacaaacc
     2221 aatcgttaag actggggaaa aagtattgaa aggtcaagtt ttagctgatg gtcctgctat
     2281 ggaacaaggt gaacttgctt taggacaaaa cgtattagta ggattcatgg aatggaatgg
     2341 atataactat gaagatgcgg ttattatgtc tgaaagatta gtgaaagatg atgtttatac
     2401 atctgttcat attgatgagt atgcaatcga atgtcgtgat actaaattag ggcctgaaga
     2461 aattactcgt gacattccta acgttgggga tgaagctcgt aaaaacctaa atagtgacgg
     2521 gatcattatg attggtgctg aggttaaaga gggtgacatt ctagttggta aagtaacacc
     2581 aaaaggtcaa gctgagcttt cggcagaaga aaaattatta cttgcaatct ttggagaaaa
     2641 atctcgtgaa gttaaagata attcattaag agttcctcat ggtggggctg gaatcgttca
     2701 tgacattaaa gtatttgaaa gaaaaaatgg tgatgaatta caaccaggtg ttaataaagt
     2761 tgttaaagtt tatattgttc aaaagagaaa aatttctgaa ggggataaaa tggcaggacg
     2821 tcatggtaat aaaggggtaa tctctaaaat tttaccaatt gaagatatgc cacacttaga
     2881 agatggaaca ccattggata ttatgttaaa cccattaggg gtaccttctc gtatgaatat
     2941 cgggcaagta cttgaattac atttaggtta tgctgctcgt caattaggtt tatatattgc
     3001 aacaccagcc ttcgatggtt tacatccaag tgatctagaa gatatcatgg ctgaagcagg
     3061 aatgtctaaa gatggtaaac aacctgttat ttctggtcgt actggtgaat attttgataa
     3121 taatatctca gttggaatca tgtatatgat taaattagcc cacatggttg atgataaatt
     3181 acatgctcgt tcagttggtc catactcact agttacgcaa caaccattag gtggtaaagc
     3241 acaaaatggt ggacaaagat ttggggaaat ggaagtttgg gcacttgaag catatggtgc
     3301 tgcatataca ttacgtgaaa tattaacagt aaaatctgac gatgttgttg gtcgtgttaa
     3361 gacttatgaa gcaattgtta aaggacaacc attaccagaa ccaggattac ctgaatcatt
     3421 tagagtgtta aagaaagaat tgcaagctct tgcattagat attcgtctat tagatgaaaa
     3481 tgataatgaa gtagatatgc gtaatatcga agaagaggaa catcgtttcc ctcgtagtat
     3541 cgataaagat gaagtgattg aaactcctga aacagaagat gaattagaag aagaaattct
     3601 tgaggatgat ttagatgttg aagaagagga agatttcgaa gatatcgacg aagattttga
     3661 tgaagaatta gaagaaatcg aagaaagtga atcattatag gaggaataaa gatggcaaat
     3721 acaaataaat tctcagcaat tcaaattggc ttagcttccc ctgaaaagat tcgcgaatgg
     3781 tcttatggtg aggttaaaaa acctgaaact attaactatc gttctcaaaa accggaaaag
     3841 gacggattat tttgtgaaaa gatttttggt ccttcaaagg actgggaatg tagttgcggt
     3901 aaatacaaaa aagtacgtta taaaggtgta gtatgtgatc gttgtggagt tgaagtaact
     3961 aaatcagctg ttcgtcgcga aagaatggga catattgaat tagcaacacc aatcgctcat
     4021 atttggtatt tgaaaggaat tccatcaaga atgggactta tcttagatat gtcaccaaaa
     4081 caattagaag aaattattta ctttgtttct tatgttgtta ttgataaagg aagtacacca
     4141 ttagaataca agcaagtatt atctgaaaga gactatcgta aatgttttga acaatttggt
     4201 catacttttg aagcccaaat tggggctgaa gcaattcaaa cattattaca acaagttgat
     4261 ctagatgctg aatttgcaaa agtaactaaa gaacttaaag aagctcaagg tcaaaagaga
     4321 acaaaattat taaaacgatt agaagcaatt gaggctttta gaacttcaac aaatgaacca
     4381 gagtggatga ttcttgaagc tttaccggta ataccacctg atttgcgtcc aatgttacaa
     4441 ttggatgggg ggcgttttgc tacttctgat ttaaacgatt tatatcgtcg tgttattact
     4501 cgtaataatc gtttaa
//
LOCUS       DAOMTA010000156         4514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_536_length_4514_cov_2.163265, whole genome shotgun sequence.
ACCESSION   DAOMTA010000156 DAOMTA010000000
VERSION     DAOMTA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_4514_cov_2.163265"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..>591)
                     /locus_tag="PLK68_05530"
     CDS             complement(141..>591)
                     /locus_tag="PLK68_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM89908.1"
                     /translation="SLEEIKKGAKIAVPNDASNTSRAYVLLQKAKYITLDPDVDISSV
                     TKDDIIKNPYEIEFPEMDSTMIPQALDEFDYAVITGSIVYNAGIDASSALLQEDVLEH
                     LLLQVVVKEKNKDTKWAKAIVEAYRSKEFKEYLDKNNNGLWYVPSYN"
     assembly_gap    592..601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<602..947)
                     /locus_tag="PLK68_05535"
     CDS             complement(<602..947)
                     /locus_tag="PLK68_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM89909.1"
                     /translation="MKKRILSLLLVMVFVLAGCQSSSDKSEDKTTIIYGKAAGPYTVL
                     FEDAIIPILKKEGYQFKCIEFSDLLQNDTALNEGEIDVNVEQHTAYMKNFNESQDGDL
                     VALTAIPTVPAGI"
     gene            complement(958..1626)
                     /locus_tag="PLK68_05540"
     CDS             complement(958..1626)
                     /locus_tag="PLK68_05540"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HRM89910.1"
                     /translation="MIENYLNIPADKLLEAAGQTIYMLGISLFFGTLIGIPLGLLLVL
                     TRKNGVKANHTIHFLVSGLVNIVRSVPFVILLVFIAPLTKVLVGTRIGTKAAIVPLVF
                     YIAPYLARLIESSILEVQPGILEAAKAMGANTLEIIRYFLLPEAKASLVLALTTGTIG
                     LLGATAMAGTIGGGGVGDLALTYGYQRFNNTLMFVTVIILIIFVQLIQTLGNHLSKKI
                     RTHE"
     gene            complement(1619..2641)
                     /locus_tag="PLK68_05545"
     CDS             complement(1619..2641)
                     /locus_tag="PLK68_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM89911.1"
                     /translation="MIKIEHVNKTFTTKSGEVPALQDVSLEVEAGDIYGVIGYSGAGK
                     STLLRMVNLLERPENGAIYINNQNIIDLKERELNILRKDIGMIFQQFNLLESQTVYQN
                     IAIPLILSKLDKKAIEVRVKQLLSFVELEDKRDTYVSQLSGGQKQRIGIARALATEPK
                     ILLCDEATSALDPQTTEAILLLLKKINCELGITILLITHEMNVIKKICNKVAVMKSGK
                     IIEKGDTLEVFSNPQQEMTKRFVETVISNVIPTTLLKELDYRYPVLKLTFFGESTKHD
                     VISKINKHFKITTTILFASVNEVGEEILGILTIQLKGEQDEVIRAINYIQKQDVKIER
                     VELNYD"
     gene            complement(2634..3788)
                     /locus_tag="PLK68_05550"
     CDS             complement(2634..3788)
                     /locus_tag="PLK68_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM89912.1"
                     /translation="MEDDSLQKELEYQFEWFHRNPELSYEEYETTKRIKTLLQEHDIE
                     VLDLPLKTGLVAVIRGGYPGKVIALRSDIDALPVNEETTLSYKSEIEGKMHACGHDFH
                     LTTIYGVALLLNENAAQLHGTVKLLFQPAEESSLGALKIIETGVLDDVNAIFGIHSTS
                     QFEVGTIGIKAGTVSAAVDRFKINLKGFGSHAAHPQMAKDPIVAAAALVNSLQTIVSR
                     NMDPFNSSVLSITHLQAGNTWNVIPEQALIEGTVRTLTSEERELFGKRLKEITYGISQ
                     AYDLDTEIEWIAGPPATVNDEEWSNFAKAIAQAEKIAIGIPEATLGGEDFAFYLEKIR
                     GTFIKIGTGKTYPNHHPKFKVNPAALSLAAKYLSQLAKQALLKLEADEND"
     gene            complement(4078..>4514)
                     /locus_tag="PLK68_05555"
     CDS             complement(4078..>4514)
                     /locus_tag="PLK68_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM89913.1"
                     /translation="LLNNVDSIIGVLGALGAGIAYTCVRKLGKQGVNGAKIVFFFSCF
                     SCLSVLPYLIINYQPMSLEQFLILLGAGLMAAGGQFAITAAYNNAAGKDISIYDYSQI
                     LFAAILGFIFLNQIPDIWSIVGYIIIIGAALGVYLYQRRIAN"
BASE COUNT         1450 a          911 c          565 g         1578 t
ORIGIN      
        1 caagcattag aattattgaa atccatggat attgatataa gccaaatcaa tttattttct
       61 attcaacaaa cgagttcatc atgaattttt aattttttag gtcggctttt tttataaatt
      121 tctttcacac ttatcccctt ttaattatag ctaggtacat accataaacc attattattt
      181 ttatctaaat attctttaaa ttcttttgaa cgataagctt caacaattgc tttagcccat
      241 tttgtatctt tatttttttc ttttacaact acttgtaaca ataaatgttc taaaacatcc
      301 tcctgtaata acgcactcga agcatcaatt cctgcattat aaactatgct gccggtgatt
      361 acagcataat caaactcatc taaagcttga ggaatcattg ttgaatccat ttctggaaac
      421 tctatttcat aaggattttt aataatatca tcttttgtca cggaactaat atccacatct
      481 ggatctaaag taatatactt tgctttttgt aataaaacat atgctctaga tgtatttgaa
      541 gcatcgtttg gtacagcaat cttagcgccc tttttaattt cctctaagct tnnnnnnnnn
      601 naaattcctg caggtacagt tggaattgct gtcaaagcta ctaaatcacc atcttgtgat
      661 tcattaaaat ttttcatgta agctgtatgc tgctcaacat tgacatctat ctcgccttca
      721 tttaaggctg tatcattttg caataaatct gaaaattcga tgcatttaaa ttgataacct
      781 tcttttttca aaattggaat aatggcatct tcaaataaga ctgtatatgg ccccgctgcc
      841 ttaccataga taattgttgt cttatcttca cttttatcac tactactttg gcatccggcc
      901 aaaacaaaaa ccatcactaa taataaactc aatattctct ttttcatttt tcttccccta
      961 ttcatgtgtt ctaatttttt tagataaatg atttcccaat gtttgaatta attgtacaaa
     1021 gataattaaa atgattacgg taacaaacat tagagtatta ttaaaacgct gataaccata
     1081 agttaatgct aaatcaccaa caccaccgcc gccgattgtt ccagccatag cagttgcccc
     1141 caataaaccg atcgttccag tagtaagtgc taaaactaac gaagctttcg cctctggtaa
     1201 taaaaaataa cgaatgattt ccaaagtatt tgcccccatt gctttagcag cttctaaaat
     1261 tcccggttga acttctaata tagaactttc tatcaaacga gccaaatatg gtgcaatata
     1321 aaatactaat ggaacaattg cagcctttgt tcctattctt gttccaacca gcacttttgt
     1381 tagcggagca ataaatacta ataagattac aaatggcaca gatctaacaa tatttactaa
     1441 cccactcaca agaaaatgaa ttgtatgatt ggctttaact ccattttttc tagtaaggac
     1501 taaaagtaag cctagcggta ttcctatcaa ggttccaaaa aataatgata ttcccaacat
     1561 ataaattgtc tggcctgccg cttcaagtaa tttatcagca ggtatattta aataattttc
     1621 aatcataatt caattccacc ctctctatct tcacatcttg tttttgaata taatttatcg
     1681 ctcgaataac ttcatcctgt tcacctttta actgaattgt taaaattcct aaaatctctt
     1741 ctcctacttc attaactgaa gcaaataaaa ttgttgttgt aattttaaaa tgcttattaa
     1801 ttttagaaat tacatcatgc tttgtacttt caccaaaaaa agtaagtttt aaaaccggat
     1861 accgataatc taattctttt aaaagtgtgg ttggaattac attgctaata actgtttcaa
     1921 caaatctctt tgtcatttct tgttgtggat tactaaaaac ctctaaagta tcacctttct
     1981 caatgatttt accagatttc attaccgcta ctttattaca aattttcttg attacattca
     2041 tctcatgagt tattaacaaa attgttatcc caagttcaca attaattttt ttaagcagca
     2101 ataaaattgc ttctgttgtc tgtggatcta aagcactagt tgcctcatca cagagtaaaa
     2161 tcttaggttc tgtagctaat gctctagcaa taccaattct ttgtttttga cccccagaca
     2221 gttgtgatac ataagtatca cgcttatctt ccaattccac aaaagataat aattgtttaa
     2281 ctcttacttc aatagctttt ttatccagtt ttgaaagaat caatgggata gcaatgtttt
     2341 gatagactgt ttgtgattct aataaattaa actgttgaaa aatcatacct atatcttttc
     2401 gtaaaatatt taattctcgt tcttttaaat ctataatatt ttgattatta atatagattg
     2461 ccccattttc aggacgttcc aataaattta ccattcgtaa taacgttgat ttaccagccc
     2521 cagaataacc gattacaccg taaatatcac ccgcttctac ttctagagag acatcttgca
     2581 atgctggaac ttcaccactt ttagttgtaa aagtcttatt tacgtgttca attttaatca
     2641 ttctcatctg cctccaattt taaaagagcc tgtttcgcca attggctaag atattttgca
     2701 gctaacgata aagctgcagg atttacctta aacttcggat gatgattagg ataggtcttt
     2761 ccggttccga ttttaataaa tgttcccctg attttttcaa gataaaatgc aaaatcttcg
     2821 cctcctaaag ttgcttcggg tatccctata gcaatttttt ctgcttgagc aattgcttta
     2881 gcaaaattac tccattcttc atcattgacg gttgcaggag gccccgctat ccactcaatt
     2941 tctgtatcaa gatcataggc ttggctaatc ccataagtta tttcctttaa tcgttttcca
     3001 aacagctccc tctcctcgct agtcagcgtt ctaacagtcc cttcaattaa ggcttgttca
     3061 ggaataacat tccacgtatt ccctgcttga agatgtgtaa tacttaaaac actactatta
     3121 aaaggatcca tatttcgact aacgatcgtt tgtaagctgt taactaatgc agcagctgca
     3181 acgattggat ctttagccat ttgtggatgg gctgcatggc ttccaaagcc cttcaaatta
     3241 attttaaatc gatcaacagc agctgaaact gtccccgctt taattccaat tgttcccact
     3301 tcaaactgcg aagtactatg aattccaaaa attgcattaa catcatctaa aacccctgtc
     3361 tcaataattt ttagagctcc taatgaagac tcttcagccg gttgaaataa taattttact
     3421 gttccatgta attgagcagc attttcattt aacaataaag caacaccata aatcgttgtt
     3481 aaatgaaaat catgaccaca agcatgcatc tttccttcaa tctctgactt atagcttaat
     3541 gtcgtttcct cattaactgg taaagcatca atatcacttc gtaaagcaat tactttacca
     3601 gggtatccac ctctaataac tgcaactaat ccagttttta atggtaaatc taaaacttca
     3661 atatcatgct cttgtaataa tgttttaatt cttttagtag tttcatattc ctcataagat
     3721 aattccggat tacgatgaaa ccactcaaat tgatattcta attctttttg taaactgtcg
     3781 tcttccattc taattccccc tttttaataa caaaagtggg atgtttatgt aaacatccca
     3841 ctaccccatt taatatagta ttaagaatat ttgcatacac gaaaatgtac cctatacttt
     3901 tgtatacagc tgcacataca catatacatc atatctcttc tgctttttaa catcatcgtt
     3961 cgctcctctt cttttcctac tatatcgata ggtttgctat gtaaagtatc ttaacacact
     4021 ataaatactc cgtcaagcaa aaaaataaat gtcaatatgt ttgcataaaa aaaatagcta
     4081 attagctatt cttcgttgat ataaataaac tcctaaagca gcaccaataa taatgatata
     4141 tccaactatg ctccatatat caggaatttg atttaagaaa ataaatccta atatcgctgc
     4201 aaataatatt tgactataat catagattga aatatccttg ccagctgcat tattatacgc
     4261 cgcagtaatt gcaaattgac caccagctgc cattaaccct gcacctagta agattaaaaa
     4321 ctgttctaaa ctcatcggct gataattaat tattaaatat ggtaacacac tgagacaaga
     4381 aaagcatgaa aagaaaaata caatttttgc accatttacc ccctgcttgc ctagcttccg
     4441 cacacaagta taagctattc ctgctcctaa tgcccccaaa acaccaataa ttgaatccac
     4501 attatttaaa agat
//
LOCUS       DAOMTA010000157         4510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_539_length_4510_cov_2.412346, whole genome shotgun sequence.
ACCESSION   DAOMTA010000157 DAOMTA010000000
VERSION     DAOMTA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_539_length_4510_cov_2.412346"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLK68_05560"
     CDS             complement(<1..197)
                     /locus_tag="PLK68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89914.1"
                     /translation="MNQSLLEQLKIITAEEQAILNNKVNIQKSIYTGNDQFIVESKKM
                     LDKQTLISVRTHTRFADFPLHR"
     gene            complement(414..1238)
                     /gene="rhaD"
                     /locus_tag="PLK68_05565"
     CDS             complement(414..1238)
                     /gene="rhaD"
                     /locus_tag="PLK68_05565"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HRM89915.1"
                     /translation="MEILKTQFIQGFMRMCSDGYKLGWHERNGGNLTYRIKVDEIALI
                     REGLDETTEDYPIGTSVPALANEYFLVTGSGKFFRNVDIDPLDSIGIIKIDTRGENYK
                     ICWGLINGGKPTSELPSHLMNHEVKKLKNENYRVIYHCHATNVIALTFILPLKDEIFT
                     RELWEMATECPVVFPDGVGVVPWMVPGGRDIAIATSELMKKYDVAIWAHHGIFCAGSD
                     FDITFGLAHTVEKSAAILVKMLSMSSTKLQTISPANFRLLAKDFNVVLPEKFLFDK"
     gene            complement(1249..>2074)
                     /locus_tag="PLK68_05570"
     CDS             complement(1249..>2074)
                     /locus_tag="PLK68_05570"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRM89916.1"
                     /translation="TLSNPDKEIREFWIRHCKACIRISEYFAKELNSPCLMNIWIPDG
                     LKDIPADRLGPRQHFMESLDEILSIDYDKEKVLVCLESKVFGIGMESYTVGSSEFTIN
                     YAQTRGILPLMDNGHYHPTEVVSDKLSAMLLFNQKVALHVTRPVRWDSDHVVLFDDET
                     KEIAKEIVRNDALDRVLVGLDFFDASINRISAWTVGMRNMQKALLNALLIPFDDLKKM
                     QSEGDFTKIMALQEELKLYPLGDVWNYICKITQVPEGLEWFKEIKKYEHEVLVNRK"
     assembly_gap    2075..2095
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2096..2501)
                     /locus_tag="PLK68_05575"
     CDS             complement(<2096..2501)
                     /locus_tag="PLK68_05575"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HRM89917.1"
                     /translation="MNVVERYKSAREIYKKVGVDTDLAIRKLLNFPISMHCWQGDDVV
                     GFDGAGALSGGIQTTGNYPGKARTPEELMADIDKVLSLVPGKHRINLHASYAIFENGE
                     VVDRDQIEPKHFIKWVEFAKERGLGLDFNPTIF"
     gene            complement(2504..3910)
                     /gene="rhaB"
                     /locus_tag="PLK68_05580"
     CDS             complement(2504..3910)
                     /gene="rhaB"
                     /locus_tag="PLK68_05580"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HRM89918.1"
                     /translation="MEYYLAVDIGASSGRHILGSYQNGKLVLEEIHRFENNIRVIDEH
                     LSWDVDYLFHEIIAGMKKCKELDKIPLSMAIDTWAVDYIFLDRHGKRIGNAISYRDHR
                     TDNIDQEVYKIVSAEELYERTGIQKQLFNTLYQLMAVKKYTPEILTQVSSMLMIPDYF
                     SYLLTGRQVCEYTNASTTQLVSSNTRNWDRELIEQLGYPQTMFKEIIEPGTIIGNLTD
                     IVQESVGFDTKVIATASHDTASAVVSVPALREDFIYISSGTWSLMGMERNIADCSLES
                     MQANFTNEGGYNHRFRYLKNIMGLWMIQSLRRELEETLSFNELCQMAKANQNFPSRVD
                     VNDCCFLSPDSMIKAIQDYCQNSNQPIPKTAGELANVIYQSLAISYAATIEEIEMLTR
                     KKYEAIYIVGGGGNAEYLNELTAKATGKVIYTGPVEATATGNIVVQMLNNQVFASLVE
                     ARKCIKNSFEIKKYKEEK"
     gene            complement(4078..>4510)
                     /locus_tag="PLK68_05585"
     CDS             complement(4078..>4510)
                     /locus_tag="PLK68_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89919.1"
                     /translation="VEISLRAFNETKYRVNFKADLNTNLMNRYPCFSGAVKYHFRYSF
                     ITKDVLLEISEAYEIVEVIVNGKLAGVKIAPEYLFDISEYLEIGENSFEIIVINTLAR
                     NQHDAMSQFLALEPMGITGTLKFYTKNVDDEVIVNRDKNLI"
BASE COUNT         1478 a          875 c          563 g         1573 t
ORIGIN      
        1 cgatgcaagg ggaagtcagc aaaacgagta tgggttctaa ctgaaatcaa agtttgttta
       61 tccagcattt ttttactttc aacaataaac tgatcattac cagtataaat tgatttttga
      121 atatttactt tattatttaa gattgcctgt tcttcagcag ttattatttt cagctgttcc
      181 aaaaggcttt gattcatatt tatcttcccc cttttattta tgataacttt gtaaattcta
      241 aaacgcaatc gatttaaact aaaataaaga ttatttaaat tattaattaa ataatcttta
      301 ttttcaaaca tgattaacca ccagcattaa aagagaggtt ttaatcaatg ataaataact
      361 ggagatattt ttgattgttg gtcaaaaaac acatagttaa tcaagacaat attttattta
      421 tcaaataaaa atttttctgg tagaacaaca ttaaaatctt tagctaacaa tctaaaatta
      481 gctggtgaga tagtctgtaa tttagttgat gacatcgata acattttgac taaaattgca
      541 gctgattttt caactgtatg agcaagaccg aaagtaatat caaagtcgct tcctgcacaa
      601 aagataccat gatgagccca gattgccacg tcatattttt tcattaattc agatgttgcg
      661 atggcaatat cacgacctcc aggaaccatc caagggacta cccctacacc atcaggaaaa
      721 acaactggac attcagtcgc catttcccat aactcccgag taaatatctc atcttttaat
      781 ggtaaaataa atgttaaagc aattacatta gtagcatgac aatgataaat gacacgataa
      841 ttttcattct ttaatttttt tacttcatga ttcattaagt gtgatggcaa ttcgctagtt
      901 ggttttccac cattaataag tccccaacaa atcttataat tctcaccacg agtatcaatt
      961 ttaataatac ctatactatc taatggatca atatcaacat ttctaaaaaa tttacctgaa
     1021 ccagttacta aaaaatattc attagctaaa gctggaacac tcgtcccaat cggataatcc
     1081 tcagttgttt catctaaccc ttctcttatt aaagcgattt catctacttt aattcgatag
     1141 gtaagatttc caccatttcg ctcatgccag cctaacttat agccgtcgct gcacattctc
     1201 ataaaacctt gaataaattg tgtttttaag atttccatta ttttactcct attttctatt
     1261 tactaaaact tcatgttcat attttttaat ttctttaaac cactctagac cttcaggtac
     1321 ttgagtaatt ttacaaatat agttccatac atcacccaat gggtataatt tcaattcttc
     1381 ttgtagcgcc atgatttttg taaaatcacc ttcagactgc atttttttca aatcatcaaa
     1441 tgggattaat aaggcattta ataaagcttt ttgcatattt ctcattccta ctgtccaagc
     1501 actaatacga ttgatgcttg catcaaaaaa atctaaacca actaatactc gatctaaggc
     1561 atcgttacga acaatttctt tagcaatctc ttttgtttca tcatcaaata atacaacatg
     1621 atcactatcc cagcgaactg gtctagttac atgcaaagct actttttgat taaataacaa
     1681 cattgcagat agtttatcag atactacttc ggtaggatga taatgtccat tatccatcag
     1741 tggcaagatt cctcttgttt gagcataatt aatcgtaaat tcactagacc caacagtata
     1801 cgattccatc ccaattccaa atacttttga ttctaaacaa accaatactt tttctttatc
     1861 ataatcaatt gataaaattt catctaatga ttccataaaa tgttgccgtg gtcctaaacg
     1921 atcagcggga atatctttta atccatcagg aatccaaata ttcattagac atgggctatt
     1981 taattcttta gcaaaatatt cagaaatacg aatacaagcc ttacaatgtc tgatccaaaa
     2041 ttcacgaatt tctttatccg gattagataa tgttnnnnnn nnnnnnnnnn nnnnnaaaaa
     2101 atcgttggat taaaatctag ccccaatcct ctttctttgg caaactctac ccatttaata
     2161 aaatgcttag gttcgatttg atcacgatct accacttcac cattttcaaa aatggcataa
     2221 ctcgcatgaa gattaattct atgtttacct gggactaatg ataatacttt atcaatatca
     2281 gccattagtt cttcgggcgt tctagccttt cctggataat taccggttgt ttgaatgcct
     2341 cccgacaacg cccccgctcc atcaaatcca actacatcat caccttgcca acaatgcata
     2401 gaaatgggaa aattaagtaa ttttcttatt gctaaatcgg tatcaacacc aactttttta
     2461 taaatttctc tagcactttt atatctttca actacattca tttttatttt tcctccttat
     2521 actttttaat ttcaaacgaa tttttaatac atttacgtgc ttcaacaagt gaagcaaaaa
     2581 cttgattatt aagcatttga acaactatat taccagttgc agttgcttct accggtcccg
     2641 tataaattac tttaccagta gcttttgctg tcaattcatt taaatactca gcattcccgc
     2701 caccaccaac aatatagatt gcttcatatt tttttcttgt tagcatttca atttcttcaa
     2761 ttgtagctgc ataacttatt gcaagactct gataaatcac atttgccagt tctccagcag
     2821 ttttaggtat cggctgatta gaattttgac aataatcttg gattgcttta atcattgaat
     2881 ctggtgataa aaaacagcaa tcatttacat ctacacgtga agggaaattt tgattggctt
     2941 ttgccatctg gcaaagctca ttaaaactta acgtttcttc tagctcacga cgcaatgatt
     3001 gaatcatcca aagtcccata atgtttttta aatatctaaa acgatgatta taaccacctt
     3061 cgtttgtaaa attagcttgc atactctcca atgaacaatc tgcaatattt cgttccattc
     3121 ccattaacga ccatgttcct gaggaaatat aaataaaatc ttctcgaaga gctggaacgc
     3181 ttactactgc tgaagcagta tcgtgactgg ctgtagcaat aacttttgta tcaaaaccta
     3241 cactttcttg aacaatatct gttaaattac ctattatggt gcctggttca atgatttctt
     3301 taaacatagt ttgtggatat cctagctgtt cgattaattc acgatcccaa tttctagtat
     3361 ttgaactcac taattgagta gtcgaggcat tagtatattc acaaacctgc ctacctgtta
     3421 acaagtaact aaagtaatca ggaatcatca gcatcgaaga aacttgggtt aaaatttcag
     3481 gcgtatactt ttttacagcc attaattgat aaagtgtatt aaataactgt ttttgaattc
     3541 ctgttcgctc atacagttct tccgccgaaa ctattttata gacttcttga tcaatattgt
     3601 cagtacggtg atcacgataa gaaatagcat tgccaattcg tttaccatgc ctatctaaaa
     3661 agatataatc aactgcccaa gtatcaattg ccatactaag tggtatttta tctaattctt
     3721 tgcatttttt cattcccgca atgatttcat gaaacaaata atcaacatcc caagataaat
     3781 gctcatcaat cactctaata ttattttcaa aacgatgtat ttcttccaaa acaagtttac
     3841 cattttgata acttcctaaa atatgtcttc cgctcgaagc accaatatct actgccaaat
     3901 agtattccat cttatacttt cctcctctat tacacccata ttataaattt aacctgtcct
     3961 cattttaagg acggtacttg tctaaaaaag tgtctttatt tgcaaaataa aatagaaagg
     4021 ccaaattacc tttctataaa aattactgtt gttaacatta atgaatgtaa tttgaaatta
     4081 aattaagttt ttatctctat ttacaataac ctcatcgtca acatttttcg tatagaactt
     4141 gagtgttccc gtgattccca ttggttcaag tgccaaaaac tgtgacattg catcatgctg
     4201 attacgagct aatgtgttga taacaattat ttcaaacgag ttttcaccaa tttcaagata
     4261 ttcacttata tcaaataaat attctggagc tattttgact cccgctaact ttccattaac
     4321 gattacttca acaatttcat atgcttcact aatttctaat aaaacatctt tagttataaa
     4381 tgaatatcta aaatgatact tgacagcacc cgaaaaacat ggataacgat tcattaaatt
     4441 agtgtttaaa tcagctttaa aatttactcg atatttagtt tcgttaaatg ctcttaaaga
     4501 aatctcaaca
//
LOCUS       DAOMTA010000158         4497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_542_length_4497_cov_2.652859, whole genome shotgun sequence.
ACCESSION   DAOMTA010000158 DAOMTA010000000
VERSION     DAOMTA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_542_length_4497_cov_2.652859"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..340
                     /locus_tag="PLK68_05590"
     CDS             182..340
                     /locus_tag="PLK68_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89920.1"
                     /translation="MLFIEYTYNQFCPNAKILDIGCGDGTIWLNNQNKISNNISITLA
                     DISLKMLE"
     gene            380..526
                     /locus_tag="PLK68_05595"
     CDS             380..526
                     /locus_tag="PLK68_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM89921.1"
                     /translation="MEEADCYYLPYLDKSFDVVIANHVFMYSDDLPKALQEINRILKD
                     DGIL"
     gene            551..892
                     /locus_tag="PLK68_05600"
     CDS             551..892
                     /locus_tag="PLK68_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89922.1"
                     /translation="MKEREVMLKDFDSKISFNQEILYQRFGYENGKTKLEKYFQDIKL
                     YDRKEVYEITDLDLYYQFILSGKGLSLNLEPLYKKKKQLYEYMQKYLNKNNLFYLTTH
                     AGMFVARKRKK"
     gene            889..1532
                     /locus_tag="PLK68_05605"
                     /pseudo
     CDS             889..1532
                     /locus_tag="PLK68_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
     gene            complement(1558..2067)
                     /locus_tag="PLK68_05610"
     CDS             complement(1558..2067)
                     /locus_tag="PLK68_05610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRM89923.1"
                     /translation="MIIRPEEKQDFNEIYTVVKAAFVTAEHSDGNEQDLVIALRTGNN
                     YIQKLSLVAKIDNQLVGYIMFTTAKVGNDTVLVLAPLAILPAFQKQGIGSALINKGHQ
                     IAKELGYEYCLVLGSEHYYPRFGYLPAEQFGIKVPKGIPSINFMAKKLIFRAGKISGE
                     VKYAKEFGI"
     gene            complement(2121..>4497)
                     /gene="gyrA"
                     /locus_tag="PLK68_05615"
     CDS             complement(2121..>4497)
                     /gene="gyrA"
                     /locus_tag="PLK68_05615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM89924.1"
                     /translation="DRAIPDVRDGLKPVQRRIIYGMSDLGCTPGTPYKKSARIVGEVM
                     GKYHPHGDFSIYEALVRMAQDFSYRYMLVDGHGNFGSIDGDGAAAQRYTEARMSKISS
                     ELVRDLKEDTIDFIPNYDGEEQEPAVLPARIPNLLINGSTGIAVGMATNIPPHNLVEV
                     IEAILAVAKDPDITIMELMENYIQGPDFPTGGYILGKTAIKKAYETGNGLIVMRAKTE
                     IEEKGSKKVIVVTEIPYMVNKARMIEKMSQLVREKKIEGITEIRDESNRDGIRVVIEL
                     RRDIQPEVILNQLYKLTSLQTTFGANCIALVNNEPKTLNLKEMITLYLDHQEEVIKRR
                     TKFRLTKAEDRAHILEGLKRALDQIDAIIDLIRSSRTTEIIQTRLMEEFNMSDKQAKA
                     IREMQLQRLAGLERQKIEDDLNKLLEIIADLKDILENRERLLSIIHDELEEIKDKYGD
                     KRRTEIIQGTFDLEDEDLIPVEDVIISLTNNGYVKRMPVDTYKTQNRGGRGIKGMATN
                     SDDIVSSLINMSTHDDLLFFTNFGKVYRLKGYNIPEFGRSAKGLPVVNLLNLDKEEQV
                     KSMISINRKQIEENNDYYLFFVTASGLVKRVHINEFERIRQSGKIAISLRDDDQLVSV
                     KLTGGNDQILIAASNGKLVRFEETNVRPMGRSASGVKGINVDGSEVIGMTTDKEGKYI
                     MVVTERGYGKMSPLEEYRLSNRGGKGVKTINATERNGQIVALRAVNGDEDLLIITDDG
                     IMIRLPMEQVKTAGRATQGVRLIKVNAENKVSSVEVVEKAAETEEDKSEEE"
BASE COUNT         1488 a          797 c          593 g         1619 t
ORIGIN      
        1 acattacatt ttgctactat tgatcattgc acctctacca attatttaat tttatcctcc
       61 atgagtttat caaagcaatt tgttcttgtc aataaaattg ttctaaagtg agaacatttt
      121 atatttattt aactattgac aggaacgtaa cgtcacttgc tagaatgaaa aagcaaggga
      181 ggtgttattt atcgaataca catataatca attttgtccc aatgccaaga tactagatat
      241 tgggtgtggt gatggaacta tttggttaaa taatcaaaat aaaatttcta ataatatttc
      301 gattacttta gctgatattt ctctgaaaat gcttgaataa tgtcaaaaca atactcaaca
      361 tttaccccaa attaaaacaa ttgaagaagc tgattgttat tatttaccat atcttgataa
      421 aagttttgat gtcgttatag caaatcatgt tttcatgtat tctgacgact taccaaaagc
      481 attacaagaa atcaatcgta ttttaaaaga tgatggaatt ctctaatgtt caactattgc
      541 taaagatatg atgaaagaaa gagaagttat gttaaaagat tttgattcaa aaatctcatt
      601 taatcaagaa attttatatc aacgctttgg ttatgaaaat ggtaaaacaa aattagaaaa
      661 atattttcaa gatataaaat tatatgatcg aaaagaggtt tatgaaataa ctgacttgga
      721 tctttattat caatttattt taagtggtaa aggtctaagt cttaatcttg agccactata
      781 taaaaagaaa aaacaactat atgaatatat gcaaaaatat ttaaataaaa ataatctttt
      841 ttatttgact acccatgccg gaatgtttgt tgcaagaaag aggaaaaaat gaagataatt
      901 ggtagtgatt ttgacggtac aattactaca aataaagtaa ttctacctga aactagaaaa
      961 caaattaaac aatttagaga tgctggtaac atctttatta tcgtaaccga aagaagtcct
     1021 cagaaattca aagatggtat taagaaatat gatattgatt tctttgatta tgtcatttgt
     1081 gctaatggag ctgttacatt agatcaacaa ctaaaagtaa taataaaata ataaccgcaa
     1141 atacgatgtc ccagagtatt gtaatcaata acctagaaga tttagaaaat ataagaaatg
     1201 aaattatttc gtttgtctta gaatttgatt ctattgccca aagaaagcag tatacatttg
     1261 gccaaattga aaatattaat gtctttcata atacccgcta tactgatatt ttagctaaag
     1321 atatttctaa agcatctgaa ttattaaaac ttttctatca attaaaagct gagcaacttt
     1381 atacaattgg agatggtgaa aatgatattt gtatgctgca atgtactgat aacagtttta
     1441 cttttaatca tgttgaaaca gttattaaaa atagtgcaca gtatcatttc gatacaatag
     1501 aacaaatgtt agcttttatt aatcaaaaat aactctcaca attaagtgag agttatttta
     1561 tattccaaat tcttttgcat atttaacttc gccactaatt ttaccagctc taaatatcag
     1621 tttttttgcc ataaaattaa ttgaaggaat tcccttaggc actttgattc caaactgttc
     1681 agctggtaaa tatccaaatc gtggataata gtgctcacta cctaaaacta aacaatattc
     1741 atatcctaat tctttagcaa tttgatggcc tttgttaatt aaagcactac caatcccttg
     1801 tttttgaaat gctggcaaaa tagctaatgg tgctaaaact aatactgtat catttccaac
     1861 ttttgctgtt gtaaacataa tataaccaac gagctgatta tcaattttag caactaatga
     1921 taatttttga atataattat taccagtcct taaagctata actaaatctt gttcatttcc
     1981 atcactatgt tcagctgtta caaaagcggc ttttactaca gtatatattt cattaaaatc
     2041 ttgtttttct tctggtctta taatcatttt attttccttt ttcaaagaat taaaagtatc
     2101 gctaagcgat acttttaatt ctattcttct tcagatttat cttcttctgt ttcagcagct
     2161 ttttcaacta cctctactga agataccttg ttttctgcat ttactttaat taaacgtact
     2221 ccttgtgtag ctcgaccggc tgttttaact tgttccattg gtaaacgaat catgattcca
     2281 tcgtcagtaa tgattaataa atcttcatcg ccattaacag cacgcaatgc aacgatttga
     2341 ccatttcgct ctgttgcatt aatagtttta acccctttac cgccacgatt tgacaaacga
     2401 tactcttcta atggcgacat cttaccataa ccacgttctg tcacaaccat gatatattta
     2461 ccttctttat cagttgtcat tccgataact tcgctaccat caacattgat tcctttaacc
     2521 ccactagcac ttcgtcccat tggccgaaca ttagtttctt caaaacgaac taatttaccg
     2581 ttagaggcgg cgattaagat ttgatcatta ccacctgtta atttaaccga aactaattga
     2641 tcatcatctc ttaagcttat cgcaatcttt ccagattgac gaattcgttc aaattcattg
     2701 atgtgaactc gttttactaa accgctagca gttacaaaga ataaataata atcattattt
     2761 tcttcaattt gcttacgatt aatactaatc atcgatttaa cttgttcttc tttatcaaga
     2821 tttaataaat ttacaacagg taaaccttta gccgaacgtc caaactctgg aatattatat
     2881 ccttttaaac gatatacttt accaaaattt gtaaagaata atagatcatc atgagtcgac
     2941 atattaatta acgaacttac aatatcatcg ctattagttg ccattccctt gattcctcgt
     3001 ccaccacggt tttgtgtttt gtatgtatct actggcattc tcttaacata tccattatta
     3061 gttaaagaaa taataacgtc ttctactgga attaaatctt cgtcttctaa atcaaatgtt
     3121 ccttgaataa tttccgtacg gcgtttatca ccatatttat ctttaatttc ttctaactca
     3181 tcatgaatga tcgataataa acgttctcta ttttctagga tatcttttaa atcagcaata
     3241 atttctaata atttatttaa atcatcttca attttttgtc tttccaaacc agctagacgt
     3301 tgtaattgca tttctcgaat tgctttagct tgtttatcag acatattaaa ttcttccatc
     3361 aatctagttt gaatgatttc agtagtacga cttgatctaa ttaaatcaat aatagcatca
     3421 atttgatcaa gagctctttt taatccttct aaaatatgag cccggtcttc agctttagtt
     3481 aatctgaatt tagtacgtcg tttaattact tcttcttgat gatcaaggta taaagtaatc
     3541 atttccttta gatttaatgt ttttggttca ttattgacca aggcaatgca gttagccccg
     3601 aaggttgtct gcaatgatgt taatttatat aattgattca aaattacttc tggttgaata
     3661 tcccgacgta attcaattac aactcggata ccatcgcgat ttgattcatc acgtatttct
     3721 gtaattcctt caattttctt ttcccgaact aactgtgaca ttttttcaat catccgagct
     3781 ttattgacca tataaggaat ttcagtaaca acaataactt ttttacttcc tttttcttca
     3841 atttccgttt ttgctcgcat gacaattaga ccatttcctg tctcataggc ttttttaatt
     3901 gctgttttac ctaaaatata accaccagtt ggaaaatcag gaccttgaat ataattttcc
     3961 attaattcca tgattgtgat atccggatct tttgcaactg ctaaaattgc ttcaatcact
     4021 tctactaagt tatgaggtgg aatattagta gccatcccaa ctgcaatccc cgttgaacca
     4081 ttaattagta agttgggaat acgagccggt aaaaccgccg gttcctgttc ttcaccatca
     4141 tagtttggaa taaaatcaat tgtatcttcc tttaaatcac gaaccagctc actagatatt
     4201 tttgacattc ttgcctcagt ataacgttgt gctgctgctc catcaccatc gattgagcca
     4261 aaattcccat gaccatcaac taacatataa cgatatgaga aatcttgagc cattctaact
     4321 aaagcttcat atattgaaaa gtcaccatga ggatgatatt tccccataac ttctccgaca
     4381 atacgggcag atttcttata tggtgtacct ggagtacatc ccaaatcact catcccgtaa
     4441 ataattcttc tttgaactgg ttttaaacca tctcgaacat ctggtatagc acgatct
//
LOCUS       DAOMTA010000159         4489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_544_length_4489_cov_2.227109, whole genome shotgun sequence.
ACCESSION   DAOMTA010000159 DAOMTA010000000
VERSION     DAOMTA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_544_length_4489_cov_2.227109"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLK68_05620"
     CDS             complement(<1..406)
                     /locus_tag="PLK68_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM89925.1"
                     /translation="MEKYIDLHMHSKYSDDGEFTPTQLVEQCHKAGIKVMAIADHNSV
                     KAIDEAKEAAAKLNIKYIPSVEIDCTYKGINLHVLGYGVDYHHADFVTLEENVLNQEL
                     ACSKEKVKLTNMLGFDVDQAALDALSDNGVYTG"
     gene            complement(407..1393)
                     /locus_tag="PLK68_05625"
     CDS             complement(407..1393)
                     /locus_tag="PLK68_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM89926.1"
                     /translation="MVKITELKTNNIHQVRLCFYNGEIWTKNDLAKHTGLSLAATTNI
                     LQLLLKDDEIKLVGEAQSTGGRKSKQYILNKDYYHLGNVSLKRDDQYYYFLVKITDLL
                     GNILKEEKLISEEGSIDELLEAISNLICDDYKVTVLALSIPGVCKDGKVGICDFEALR
                     NCELLKLLKEHFNLDIIMDNDVNAASIGFGIHYPNANNLALMYQPKIKYVGCGILINH
                     RLCSGYSNFAGELSYLPFMSHHEQDEMLFKAPNDLLLKQLATICCVINPEIIGVCSDV
                     FKEFDSSQLINYLPAEHWPKIIDIDNLDQLIKDGLYSLGIEVLKNKMRKRDR"
     gene            1524..2081
                     /locus_tag="PLK68_05630"
     CDS             1524..2081
                     /locus_tag="PLK68_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRM89927.1"
                     /translation="MSDSLITKRALAKTLKELCQYRNFEKISINDLTNKCGLNRQTFY
                     YHFQDKYDLLQWLYYDELFADIENIITFDNWDQCLLTVLEKIYQEKDFYISTINTNEQ
                     YFYHDLYNLAQKCFYDAITKLDVNKTVSPQEKNFFSEFYAYGIAGTVLRWIKTNMKNE
                     PAKLAHGLKKIATQSETFAASLLNN"
     gene            2192..3877
                     /locus_tag="PLK68_05635"
     CDS             2192..3877
                     /locus_tag="PLK68_05635"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HRM89928.1"
                     /translation="MKTKTKLLTGAALLAGAGAVAAKKYADNKDTVRKPVEITNQQFY
                     LIGGGLASMAAAAYLIQDGHVDGKNIHIFEGMKILGGSNDGAGSIKDGFVCRGGRMLN
                     EETYENFWELFDRIPSLDHPGQSVTKEILDFDHLHPTEARARLIDRHGKILDVKSMGF
                     DNNDRLTLGKLMITPESKLDDITIEQWFKDAPHFFTTNFWYMWQTTFAFQKWSSVFEL
                     KRYMNRMIFEFPRIETLAGVTRTPYNQFESVILPIKKYLDSHHVNFVTNATVTDIDFK
                     DDDTITVKVLYLNKDGKDEKIILNDNDICIMTNACMTDSATLGDYKTPAPKPLEKPIS
                     GELWYKVAQKKPNLGNPEPFFGNIKETNWESITVTFKGNEFLKIIEEFSTNIPGSGAL
                     MTFKDSSWLMSMVVAAQPHFKAQDANTTIFWAYGLYTDRLGDYIKKPMKDCTGEEIFD
                     ELLYHLHLIDRKEEIKKDIINVIPCMMPYVDAQPRKMSDRPHVVPKGSTNFAMISQFV
                     EIPEDMVFTEEYSVRAARIAVYTLLGINKPICKVTPYNKDPKVLKKALETAYR"
     gene            complement(3890..4006)
                     /locus_tag="PLK68_05640"
     CDS             complement(3890..4006)
                     /locus_tag="PLK68_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM89929.1"
                     /translation="MNVVLTGDNTNITPYIVIMVIAIVVIAGVLIYRKKKQK"
     gene            complement(4131..>4489)
                     /locus_tag="PLK68_05645"
     CDS             complement(4131..>4489)
                     /locus_tag="PLK68_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89930.1"
                     /translation="DDQQIDRLTKNGSSILGLFKVIVENERFVLMPVNHLNAKDRSVY
                     FNEIEKVKMTLSTGAATRITINRAIIYQIDLNIYFHDGSLFTLEFRSFRGVVSLIKKF
                     AELHIYVYDPMNLFNG"
BASE COUNT         1522 a          798 c          629 g         1540 t
ORIGIN      
        1 cccccgtata aacaccatta tcacttaaag catctaacgc tgcttgatca acatcaaaac
       61 ctaacatatt tgttaatttt accttttctt ttgaacatgc tagttcttgg ttcaatacat
      121 tttcttctaa cgtcacgaaa tccgcatgat gataatcaac cccataacct aaaacatgta
      181 ggttgattcc cttataagta cagtcaattt cgacactagg aatatattta atatttaatt
      241 tagctgctgc ttctttcgct tcatcaatcg ctttaacact attatgatca gcaattgcca
      301 taaccttaat tcctgctttg tggcattgtt caactaattg agtcggagta aattcaccat
      361 catcactata ttttgaatgc atgtgtaaat caatatactt ttccatttat ctatctctct
      421 ttcgcatttt attttttaat acttcaattc ctaaactata caatccatct ttaattaatt
      481 gatctaaatt atcgatatca ataattttag gccaatgttc tgcgggtaaa taattaatta
      541 attgactcga atcaaactct ttaaatacat cactgcaaac accaataatt tccggattga
      601 taacacaaca aatcgttgct aattgtttta atagtaaatc atttggagct ttaaacaaca
      661 tttcatcttg ctcatgatgg ctcataaacg gcaaatatga tagttcaccc gcaaagttag
      721 aatatccgct gcataatcga tgattaatta aaataccaca tccaacatat ttaatcttag
      781 gctgatacat taacgctaaa ttgttagcat ttggataatg aattccaaac cctatgctag
      841 ccgcattaac atcattgtcc attattatat caagattgaa atgctctttc agcagcttta
      901 acaactcaca atttcgtaac gcttcaaaat cacaaattcc aacttttcca tctttacaca
      961 ctcccgggat acttagtgct agcactgtta ctttataatc atcacaaatt aaattactta
     1021 tggcttctag taattcatcg attgaaccct cttcactaat aagcttttct tcctttaaaa
     1081 tattccctaa taaatctgta attttaacaa gaaaataata atattgatca tctcttttta
     1141 aacttacgtt acctaaatga taataatctt tatttagaat atattgtttt gatttgcgac
     1201 caccagttga ctgcgcttct ccaacaagct taatttcatc atcttttagt aataattgta
     1261 agatgttcgt tgttgctgca agtgatagac cagtatgttt agctaaatcg tttttagtcc
     1321 aaatttcacc attataaaaa cataatcgta cttgatgaat attattcgtt tttaattctg
     1381 taattttaac caaatatctc cccccttttt ttgcctttcg tcacaataaa atgaaagatt
     1441 atatacttat tataacaaga ttattattaa tgtaaatgtt ttattcataa aaatttgtta
     1501 caataatcat cgaggtgaac tatatgtctg attctttaat cactaaacgg gctttggcta
     1561 aaactttaaa agaactttgt cagtatcgaa actttgaaaa aatttccatt aacgatctaa
     1621 ctaataaatg tggtctaaat cgtcaaactt tttattatca cttccaagat aaatatgatt
     1681 tattacagtg gttatattat gatgaattat ttgcagatat cgagaatatc attacttttg
     1741 acaactggga tcaatgtcta ttaacagttt tagaaaaaat atatcaagaa aaagattttt
     1801 atattagcac tatcaatacc aacgaacaat atttttatca tgatctatat aatctggctc
     1861 agaaatgttt ttatgatgcg attactaagc ttgatgtaaa caaaaccgtt tccccacaag
     1921 aaaagaattt ttttagtgaa ttttatgctt atggaattgc cggaacagta ctccgctgga
     1981 taaaaaccaa tatgaaaaat gagcctgcaa aacttgcaca cggactaaaa aaaatcgcta
     2041 ctcaaagtga aacttttgct gctagtttat taaataatta gacaattaat tatatctgtc
     2101 tagtatttag acaaagttaa atctttgttt aaatactttt tattttttct ggctatacta
     2161 gtatttgtta gaagaaagaa ggatattaaa aatgaaaact aaaacaaaat tattaactgg
     2221 agcagctctt ctagctggag ctggagcagt tgcagcaaag aaatacgctg ataacaaaga
     2281 tacagttaga aaacctgttg aaattacaaa ccaacaattt tatttaattg gtggcgggct
     2341 tgccagtatg gccgcggctg cctatttgat tcaagacggt catgttgatg gtaaaaatat
     2401 tcatattttt gaaggcatga aaattcttgg tggtagtaat gatggtgccg gatcaattaa
     2461 agatggattt gtttgtcgtg gcggacgaat gttaaatgaa gaaacttatg agaatttttg
     2521 ggaattattc gatcgaattc cttcacttga ccatcctggt caaagcgtaa ctaaagaaat
     2581 actagatttt gatcatttac atccaactga agctcgggca cgtttaattg atcgtcatgg
     2641 aaaaattctt gacgttaagt caatgggctt tgataataac gatcgcttaa ctttaggaaa
     2701 attaatgatt actcctgaat ctaaacttga tgatattacg attgaacaat ggtttaaaga
     2761 tgcacctcat ttctttacaa ctaacttctg gtatatgtgg caaacaactt ttgcctttca
     2821 aaaatggtca agtgtttttg aattaaaacg ctatatgaac cgcatgattt ttgaattccc
     2881 acgtatcgaa acattagccg gcgtaacaag aacaccatat aatcaatttg agtcagttat
     2941 tttaccaatc aagaaatact tagatagtca tcatgtcaac tttgtaacaa acgctactgt
     3001 tacagatatt gattttaaag atgatgatac tattactgtt aaagtgcttt acttaaataa
     3061 agatggcaaa gatgaaaaaa tcattctcaa tgataatgac atttgtatta tgactaatgc
     3121 atgtatgact gacagtgcta ccttaggaga ttacaaaact cctgctccta aacctcttga
     3181 aaaaccaatt tctggcgaac tctggtataa ggttgcccaa aagaaaccaa acctcggtaa
     3241 tcctgagcca ttctttggta acattaaaga aacaaactgg gagagtatta ctgtgacatt
     3301 taaaggaaat gaattcctta aaataattga ggaattctca actaacatcc ctggtagtgg
     3361 tgctttaatg acctttaaag actcatcatg gctaatgtcg atggtcgttg cagcccaacc
     3421 gcatttcaaa gcacaagatg ctaacactac aattttttgg gcttacggct tatacactga
     3481 tcgtttaggc gactatatta aaaaaccaat gaaagattgt actggagaag aaatttttga
     3541 tgaattacta tatcatcttc atttaattga tcgtaaagag gaaattaaaa aagacatcat
     3601 caatgtcatt ccatgtatga tgccatatgt agatgcccag cctcgaaaaa tgagcgatcg
     3661 acctcatgtt gttcccaaag gatctactaa ctttgcgatg atttctcaat ttgtagaaat
     3721 ccctgaggat atggtcttta ccgaagaata ttctgtaaga gccgcaagaa ttgcagttta
     3781 tacattacta ggaatcaata aaccaatctg taaggtcaca ccatataata aagatcctaa
     3841 agtcttgaaa aaagctttag aaaccgctta tcgctagcgg tttctttttt tatttttgtt
     3901 tctttttgcg ataaatcaat actcctgcga taacaacgat agcaattacc ataattacta
     3961 tgtatggtgt aatatttgta ttatcacccg ttaacactac attcatttat attccccctt
     4021 ttcctcatta cgctttaaca tagcgatctt tttttcgcat ttcttcaact tgaaattgtt
     4081 ttttaatttt ctcaaggtta attgcaaaat atttttcaat cgcccaacga tcatccattg
     4141 aataaattca tcggatcata tacatagata tgtaattctg caaatttctt tatcaatgat
     4201 acaacccctc gaaaacttct aaattctaaa gtaaataatg aaccatcatg aaaataaata
     4261 tttaaatcaa tttgataaat tatcgcacga ttaatagtga ttcgtgttgc tgcacctgtg
     4321 gataaagtca tctttacttt ttcaatctca ttaaaataaa cgctcctatc tttagcattt
     4381 agatgattaa ccggcattaa tacaaaacgc tcattctcca caatcacctt gaataatcca
     4441 agaatactac taccgttttt tgttaacctg tcaatttgct gatcatcat
//
LOCUS       DAOMTA010000160         4488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_545_length_4488_cov_2.645387, whole genome shotgun sequence.
ACCESSION   DAOMTA010000160 DAOMTA010000000
VERSION     DAOMTA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_545_length_4488_cov_2.645387"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="PLK68_05650"
     CDS             <1..1785
                     /locus_tag="PLK68_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRM89931.1"
                     /translation="FSNLYLGETVDNNLEANYYAQVQIDDCKRNLVLSEIPKIKRCGD
                     ILPQSFINQSDGIIVDFGKMISGFINFACTAHKNQEIVLQYSEAMVDNHLVYSTCLAG
                     SVGERIGEHVIAGGTGAPAIAIQTDRLICKEGYNQFTNKFTYHSFRFVRITGIELTQL
                     QQIYATYVHTDLKKIAKITVDNQELQQLYDAATRTKLNNVHGTFEDCARERLGYGGDM
                     VALATSNLYTFELDKLYKKIIKDFRFEQTAMGGIPETAPYMGIQSNGTAKGEGPILWQ
                     LVYPYLVYKHYQYYGDLSLLKQEYPYLKKQLDYLLDYNLNKLVECCLGDHGSILIAGQ
                     FRKPTPDKLLLGYCTVLLFLRYNILIYQAIKKDTVFYQQRYQELKILTINKFKNSDGS
                     FGEGSQSGYAFAIELGLDDPKKLCALFVEKVKKDDYVFNSGIFAMALSYEILSKYGYD
                     EVIENWLLRKEAPSYQQMLKSGNQVLAELFVGEHLSLNHAMFASYQQWYYQGLAGIKI
                     TDQAVGFDHIVFNPYFSKKVNDFTCQLDTKQGLITSSWHRCGHEINWELVVPVKKVNY
                     QIKINNRYQEIKRISKNNQIIIKLIDSV"
     gene            complement(1895..2875)
                     /locus_tag="PLK68_05655"
     CDS             complement(1895..2875)
                     /locus_tag="PLK68_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89932.1"
                     /translation="MDSFKLDHFLRTYSRTEIKHKNGFATQYATKGKYRLINNIYQFN
                     TDNVPDSDTIIVYKNQRFDDVPTHVHDHIEINYMYSGSCSQIIDGHEVILKKGQMTLI
                     DTRTPHAIGYTDENDILINFIIKKDYLNNSFFSKLTDNNLITSFFINAINQDNTDLNY
                     LIFNTENNQRLQMFILEFIYEYFYPTLNSTEIKKSLFVLIILEMINTLDTSIILESIT
                     KSSTIIILALQYLEKNFLTCSLDSTAKYLNINPCYLTTLLKKNFNYSYKELIIKLKMQ
                     YASKLLLNSSYSIDQIAHECGYQNLSFFYKKFKETYYCLPKEYRNRNKKR"
     gene            3020..>3797
                     /locus_tag="PLK68_05660"
     CDS             3020..>3797
                     /locus_tag="PLK68_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HRM89933.1"
                     /translation="MIREDIMERLVCFSDEEINNLNGFVGIDKSIFISEQSNVVDADK
                     LLKANQQFAVRKHARFCEYPKHRHNYLEFMYVYGGEMVTIIDDQEIVIKQGELLLLNQ
                     NIEHAIKYTNENDIIFNFIIKPEFLEFLSGMAEEQNEVFSFIFDALYSYDNKGEYLIF
                     KVSNNEIVRNHIEAIITNIYQQQLNHSFTLKLLVGLLLTELMNNPHLIETYESNNYNK
                     LVVISILKYITLNYQEGSLSVLAKQIHQPDYKICKLIKEHT"
     assembly_gap    3798..3807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3808..3995
                     /locus_tag="PLK68_05665"
     CDS             <3808..3995
                     /locus_tag="PLK68_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM89934.1"
                     /translation="TFKQLIQEERLKAAANLLKTTSLPIVEIMQEVGYENITYFYKIF
                     KEKFKITPSIYRNHNLR"
     gene            4112..4270
                     /locus_tag="PLK68_05670"
     CDS             4112..4270
                     /locus_tag="PLK68_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HRM89935.1"
                     /translation="MAKKVDYTQLAKEVIRAVGGKENINGVTNCMTRLRFVLKDDSIP
                     NSEEVKLI"
     gene            4286..>4488
                     /locus_tag="PLK68_05675"
     CDS             4286..>4488
                     /locus_tag="PLK68_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HRM89936.1"
                     /translation="MNKGGQYQVIIGTHLNEVIKFVNQELGFKGDEKATEDKVEEKGN
                     LFNRFFKVISGCIMPMIGPMLQGG"
BASE COUNT         1596 a          510 c          798 g         1574 t
ORIGIN      
        1 tttagtaatc tttatcttgg cgaaacggtc gataataatt tagaagcaaa ttattatgcc
       61 caagttcaaa tagatgattg taaacgtaat ttagttttaa gcgaaattcc taaaatcaag
      121 cgatgtggtg atatattacc acaatcattc attaatcaaa gtgatgggat aatcgttgat
      181 tttggtaaaa tgatttcagg ttttattaat tttgcttgta cagcccataa aaaccaagaa
      241 atagtattgc agtatagtga agccatggta gacaatcatt tagtctattc tacttgttta
      301 gctgggagtg taggggagcg aattggtgaa catgtaattg ccggagggac cggggctccg
      361 gcaatagcga ttcaaacaga tcgtcttatt tgtaaggagg ggtataatca gtttacaaat
      421 aagtttacgt atcattcttt tcgctttgtt cggattactg gaattgaatt gacccagttg
      481 caacagatct atgcaacgta tgtgcatacg gatttaaaaa aaattgccaa aattacagtt
      541 gataatcaag agttacaaca attatatgat gctgccacaa gaactaaatt aaataatgtt
      601 catggaacat ttgaggattg tgctcgtgag cgtttaggct atggggggga tatggtagcc
      661 cttgctactt caaatctata tacttttgag ttagataaat tatataaaaa gattattaaa
      721 gattttcgat ttgaacaaac tgccatgggt ggaattcctg aaactgctcc atatatggga
      781 attcaatcta atggtacagc aaaaggagag ggaccgattt tatggcaatt agtttatcca
      841 tatcttgtct ataaacatta tcagtattat ggcgatttga gtttactcaa gcaagaatac
      901 ccatacttaa aaaaacaatt ggattatttg ctagattata atttgaataa actagttgag
      961 tgttgtttag gggatcatgg gagtatttta attgctggtc aatttcgcaa accaacaccg
     1021 gataaacttc tattaggtta ctgcacggta ctattatttt tacgctataa tattttaatt
     1081 tatcaagcga ttaaaaaaga tactgttttt tatcaacagc gatatcaaga actcaagata
     1141 cttacgataa ataagtttaa aaattctgat ggtagttttg gtgaaggaag tcaaagtggt
     1201 tatgcttttg caattgaatt agggctggat gatcctaaaa aattatgtgc tttatttgta
     1261 gagaaagtaa aaaaggatga ttatgtcttt aatagtggta tttttgcgat ggctctaagt
     1321 tatgaaattt tatctaaata cggatatgat gaggtaatcg aaaactggtt attgcgtaaa
     1381 gaggcaccaa gttatcaaca aatgcttaaa agtggtaatc aggttttagc agaattattt
     1441 gttggtgaac atttatcatt gaatcatgca atgtttgcaa gctatcaaca atggtattat
     1501 caaggattgg caggtataaa gattactgat caggcagttg gattcgatca tatagtattt
     1561 aatccctatt tttccaaaaa agtaaatgat tttacatgtc aacttgatac taaacaaggg
     1621 ctgattactt cttcttggca tcgatgtggt catgaaatta attgggaatt agtagttccg
     1681 gtaaaaaaag taaactatca aattaaaatt aataatagat atcaagagat aaaaaggatt
     1741 agtaaaaata accagattat cattaagtta attgacagcg tataaattaa aagtaatcag
     1801 agatgttttt taaagaacaa atctttagat gaaatcaaaa gtttgttaat aaaattaaaa
     1861 agatagaaat tttgaaatta acctcactat ttttctatct ttttttgttt ctgttacgat
     1921 attcttttgg taaacaataa taggtttctt taaatttttt atagaagaaa cttagatttt
     1981 gataaccaca ctcatgagct atttggtcaa ttgaataact actattgagc agcagttttg
     2041 atgcgtactg catttttaat ttaataatta attctttgta agaataattg aaattctttt
     2101 tcaataatgt tgttagataa caaggattga tgtttaaata cttagctgtt gaatcgaggc
     2161 tgcaagtaag gaaatttttt tctaaatatt gtaatgctaa aatgatgatt gtactggatt
     2221 tagtaatgga ttctaagatg atagaggtat ctagagtatt gatcatttct aagataatta
     2281 agacaaaaag actttttttt atttcggttg aatttaaggt tggataaaag tattcataga
     2341 taaattcaag aataaacatt tgcaatcgtt gattattttc tgtattaaag atcaagtaat
     2401 ttaaatctgt gttatcttga ttaattgcat taatgaaaaa tgatgtaatt aagttattat
     2461 cagttaattt agaaaagaaa ctattattta gataatcttt tttgataatg aaattaatca
     2521 aaatatcatt ttcatcagta tagccaattg catgtggcgt tctggtatca attaaagtca
     2581 tttgtccttt ttttaaaata acttcatgcc catcgataat ttggctacag cttccagagt
     2641 acatataatt tatttcaata tgatcgtgga cgtgagttgg aacatcatca aagcgttgat
     2701 ttttatagac tataatagta tcggagtctg gcacattatc ggtattaaat tgatagatat
     2761 tattgattaa acgatattta cctttagttg catattgagt tgcaaatcca tttttatgtt
     2821 tgatttctgt tcgactatat gttcttaaaa aatgatctaa tttaaaacta tccatcgttt
     2881 tcctccttat cttttttatt atatcactaa atctaaagtt tcgtcagtaa attactaaat
     2941 agacgttata gtatcttatt tttatattgt tagaatataa agtatagata tgatagcgtt
     3001 tactaataga ggtgatgaaa tgatcagaga agatattatg gaacgattag tttgttttag
     3061 tgatgaagaa attaataatt taaatggttt tgtaggaatt gataaaagta tttttataag
     3121 tgaacagtca aatgtagttg atgctgataa attattaaaa gctaatcagc agtttgctgt
     3181 tcgtaaacat gcacgttttt gtgaatatcc aaagcatcgt cataattatt tagagtttat
     3241 gtatgtttat ggtggtgaaa tggttactat aattgatgat caagagattg tgattaaaca
     3301 aggagaatta ttattactaa atcaaaatat tgaacatgca attaaataca ctaatgaaaa
     3361 tgacattatt tttaatttta ttattaaacc agagtttctt gaatttttat caggtatggc
     3421 agaagaacaa aacgaggttt ttagctttat ttttgatgct ttgtattcat atgataataa
     3481 gggtgaatat ttaattttta aggttagtaa taatgaaatt gtccgtaatc atattgaggc
     3541 aatcataact aatatatacc agcaacaatt aaatcatagt tttacattaa aattattagt
     3601 gggattattg ttgacagaat tgatgaataa tccacacttg attgagacat acgagagtaa
     3661 taattataat aaactagttg ttatctctat tttaaaatat attacactta attaccaaga
     3721 aggatcgtta tcagtgttag ctaaacagat tcatcagcca gattataaga tttgtaagtt
     3781 gatcaaagaa catacggnnn nnnnnnncta cttttaaaca gttgattcaa gaagaacgtt
     3841 taaaagcagc ggccaatctt ttaaagacta ccagtttgcc aattgttgaa ataatgcagg
     3901 aagttggcta tgaaaatatt acgtacttct ataaaatttt taaggaaaaa tttaaaataa
     3961 cacctagtat ttatcgtaat cacaatcttc gctaagcaaa ataggataat atcattgcca
     4021 agattagata ttatttgtaa ccgtttaact tgctattatg aatatgtcga aaataagaca
     4081 agtaaaaata ttttaaagag gagatgtagt tatggctaaa aaagtagatt acacgcagtt
     4141 agctaaagag gtaatcagag cagttggggg aaaagaaaat attaatggtg ttactaattg
     4201 tatgacccgt ttaagatttg tattaaaaga tgatagtata ccaaatagtg aagaagtcaa
     4261 gctaatttag gatgtaaaag gagttatgaa taagggaggt caatatcagg tgattattgg
     4321 tacccatctt aatgaggtaa ttaaatttgt taatcaagaa ctaggcttta agggtgatga
     4381 aaaagcaact gaggataaag ttgaagaaaa aggcaatttg tttaatcgtt ttttcaaagt
     4441 aatcagtgga tgtattatgc caatgatagg gccaatgttg cagggggg
//
LOCUS       DAOMTA010000161         4481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_549_length_4481_cov_1.903977, whole genome shotgun sequence.
ACCESSION   DAOMTA010000161 DAOMTA010000000
VERSION     DAOMTA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_549_length_4481_cov_1.903977"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1171
                     /locus_tag="PLK68_05680"
                     /pseudo
     CDS             247..1171
                     /locus_tag="PLK68_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
     gene            1306..>2325
                     /locus_tag="PLK68_05685"
     CDS             1306..>2325
                     /locus_tag="PLK68_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRM89937.1"
                     /translation="MLIQKRNGLFQDYDNNKIETAIKKAFCSLSETIKDDELKKMIAD
                     IEKHIIADMSVEAIQDLVEETLMKHGYLQVAKAYILYRQKHTEQRLIIDELLALLGDQ
                     NLKKVLLKIHKDYPQEEYSLQLLLVKFKTFYKEGMSHFEALKMLMKASVELISKEAPK
                     WEMISARFLTYDVNQQVDQKMNTAGRYTFYERINYLAQQGYYGQYILESYSKDEIDML
                     GQYISESRNELFTYSGLELIVKRYLIQNHDREIIEKPQEMFMSIAMHLAMNESNRVKW
                     AKEIYDILSTLKVTMATPTMSNARKPFHQMSSCFIDTVDDSLAGIYKSIDNFAKVSKN
                     GGGMGL"
     assembly_gap    2326..2354
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2355..>3557
                     /locus_tag="PLK68_05690"
     CDS             <2355..>3557
                     /locus_tag="PLK68_05690"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRM89938.1"
                     /translation="SSIRGFKGAAGGVIRWLKLVNDTATAVDQLGVRQGAAAVYLDAW
                     HKDLPEFLQIRTNNGDDRMKAHDIFPAVCYPDLFWKLAKNDLEASWYMMCPHEIHEVK
                     GYYLEDCYGEEWERKYYECVEDNRIPKRVMTVKEIVRLIIKSLVETGTPFTFNRDHVN
                     NFNPNPHQGMIYCSNLCTEIAQNMQAMEILPSEEIEVNGETIIVEKTKPGDFVVCNLA
                     SLTLGHLDVLNDDELRHVISVVIRALDNVIDLNYYPIPFAKITNQKYRAIGLGTSGYH
                     HMLVKLKMSFESDEHLQFIEQLYEKINYFALEASCDLAKEKGSYSLFNGSDFETGAYF
                     IKRRYQSKAWQNLQAKIKENGLRNGYLLAIAPTSSTSIIAGTTAAVDPIMKKYFMEEK
                     KGSMITRVA"
     assembly_gap    3558..3567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3568..3821
                     /locus_tag="PLK68_05695"
     CDS             <3568..3821
                     /locus_tag="PLK68_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89939.1"
                     /translation="LDSETFWLYKNAHYIDQEWIVKAAGIRQRHLDQSQSVNLYMTNE
                     FTFRKLLNLYIKAWEYGVKTLYYVRSQSLEVEECESCSS"
     gene            3831..>4234
                     /locus_tag="PLK68_05700"
     CDS             3831..>4234
                     /locus_tag="PLK68_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRM89940.1"
                     /translation="MELKQKALFNAKGDIDVRKRRMINGNTTNLNDFNNMKYQWVSDW
                     YRQAMNNFWIPEEINLTQDIRDYRLLSEAERTAYDKILSFLVFLDSLQTANLPNIGEY
                     ITANEINLCLTIQAFQEAVHSQSYSYMLDSICS"
     assembly_gap    4235..4244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4245..>4481
                     /locus_tag="PLK68_05705"
     CDS             <4245..>4481
                     /locus_tag="PLK68_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRM89941.1"
                     /translation="TKRDEILYQWKFDEHLLKRNEFIGEQYNAFLEHKDSFHLMKTIM
                     ANYILEGIYFYSGFMFFYNLGRMGKMSGSAQEIRY"
BASE COUNT         1600 a          540 c          850 g         1442 t
ORIGIN      
        1 atgatgatgg tagcggaaca tataatcgat ataaaaagaa aagttttgat tggatgaaag
       61 aagttattgc aacaaatgga gaaaagatat aaggagggaa ataaaattcc ctttttttaa
      121 attttaatcg ggtctattta taatttaggt taatatattt aaaggaggaa tgattaggag
      181 gaatcataga tgtgtacagc aataaagata tattcagata atggagatat ttattttggt
      241 cgaacaatgg atttttcatt tgaattaaat cctgaacttt attatattcc tgcaaaatac
      301 cagtggcgca atgttctaaa tactcatgat attgaaaata agtacaatat tcttggtatc
      361 gggcaagatg tctctcctgt tgctttaagt gatggtgtta atgaccatgg atttgcagtt
      421 gcggcattat atttttctgg gtttgctcaa tatgatgggc taaaaaaaga taatacatca
      481 attatcccga tcgctgcttt tgaactagtt tattttttat taagtcaatg tgcttcagta
      541 aaagaagctc aagaattaat aaaagtcatc aaaattattg gtgtagaaga tagtattaca
      601 catatagcag ctcctcttca ttggataata agcgatgcaa ataacaattg tatggtgatt
      661 gaaaaaacaa tagatggtct acatgcgatt aataatccta ttggtgttct ggcatatgac
      721 taatttacga aattattaca atttatcgcc atatcaattt caagaagcaa tatggaacaa
      781 tataaaactt actccatttg gacaaggtgc aggaacatta ggtttaccag gggattatac
      841 accaccatca cgttttatta gagcagcatt tcaaaaaaca tatacagata ttccaattga
      901 tcgtcatcaa gctgtgataa cttgtttccg gatcatggaa acggtcagta ttcctaaagg
      961 ggtcgtcatt acagcagatc aaacttctga ttatactcaa tatacgatgt ttattaactt
     1021 agcaactcga gaatattatt ttaaaacata ttggaataat cagataacaa gagttgaatt
     1081 tcctcaatat gatgaaaaag atatgaaaat gctttcattg ggaccgttaa atcaaacgat
     1141 tgaatttaaa actagaagta tttttttata aaaggatgtg ttgttagata catctttttt
     1201 tgtttatatt tatgattatt gtgaaagaat aaaatatgct taagcatatt cgttgaagat
     1261 aatcgaatga ctatgctaga atggcattct aaggatgtga aaaaaatgtt gatacaaaaa
     1321 agaaatggtc tttttcaaga ctatgataac aataaaatag aaacagcaat aaaaaaagct
     1381 ttttgcagtt tatctgaaac aataaaagat gatgaattaa aaaagatgat tgcagatatt
     1441 gaaaaacata ttatagctga tatgtctgtt gaagcaattc aagatttggt tgaagaaaca
     1501 ttgatgaaac atggatatct tcaagtagca aaagcttata ttttatatcg tcaaaagcat
     1561 acggagcagc gattgataat tgatgaactg cttgccttat tgggagatca aaatctaaaa
     1621 aaggttctat tgaagattca caaagactat cctcaagaag aatattcatt gcaattatta
     1681 ttagtgaaat ttaaaacatt ttataaagag ggtatgagtc attttgaagc gttgaaaatg
     1741 ttgatgaaag cgagtgtgga attaattagt aaagaagcac ctaaatggga gatgatttca
     1801 gctcgatttt taacttatga tgtaaatcaa caggttgacc aaaaaatgaa taccgctggt
     1861 agatatactt tttatgagcg gattaactat ttagcacaac aaggttatta tggacaatat
     1921 attcttgaaa gctattcaaa agatgaaata gacatgttag gacagtatat tagcgaatcg
     1981 cgaaatgaat tatttacata tagtggtctt gaattgattg ttaaaagata cttgattcaa
     2041 aatcatgacc gcgaaattat tgaaaaacca caggaaatgt ttatgtcaat tgctatgcat
     2101 ttggccatga atgaatcaaa tagagttaag tgggcaaaag agatttatga tattttaagt
     2161 actttaaaag tgacgatggc aacaccaact atgtctaatg cgagaaagcc atttcatcaa
     2221 atgagttcat gttttattga tactgtcgat gattcattag caggaatata taaaagtata
     2281 gataattttg ctaaagtttc aaaaaacggt ggtggaatgg gactgnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnaagtag tatcagaggt ttcaaaggag cagcaggagg tgtgattcga
     2401 tggcttaaac ttgtaaatga tactgcaaca gcagtagatc aattaggagt tcggcaggga
     2461 gcagcagcag tatatttgga tgcatggcat aaagatctac ccgaattttt acaaattaga
     2521 acaaataatg gcgatgatcg aatgaaagca catgatattt ttcctgcggt ttgttatcca
     2581 gatttatttt ggaaattagc taaaaatgat ttggaagctt catggtacat gatgtgtcca
     2641 cacgagatac atgaagttaa aggttattat ttagaggatt gttatggtga agagtgggaa
     2701 aggaaatatt atgagtgcgt tgaagataac cggattccta aacgcgttat gacagttaaa
     2761 gaaattgttc gtttaattat taaatcacta gttgaaacag gaactccttt cacatttaat
     2821 cgcgatcatg ttaataattt taatcctaat cctcatcagg gaatgatata ttgcagtaat
     2881 ctttgcacag aaattgctca aaatatgcaa gcgatggaga tcttaccgtc tgaggaaatt
     2941 gaggttaatg gtgaaactat tattgttgaa aaaacaaaac caggtgattt tgtagtttgt
     3001 aatttagctt cattaacatt aggtcatctt gatgtcttaa atgatgatga attgagacac
     3061 gttatcagtg ttgtaattag ggctttggac aatgtaattg atttaaatta ttatccaatt
     3121 ccttttgcca aaataactaa tcaaaaatat cgtgcgattg gattaggaac aagtggttat
     3181 catcatatgc tggtaaaatt gaaaatgtca tttgaaagtg atgagcattt acagtttatt
     3241 gagcagcttt atgaaaagat taattacttt gcgttggaag cttcatgtga tttagctaaa
     3301 gaaaaaggaa gttactcatt gtttaatggt agtgattttg aaacaggtgc atactttata
     3361 aaacgtcgct atcaaagtaa agcatggcaa aatcttcaag ctaaaatcaa agaaaatggt
     3421 ttaagaaatg gatatctttt agcgattgcc ccaactagtt caacttcaat tattgctgga
     3481 acaacagcag cggttgatcc tatcatgaaa aagtatttta tggaagagaa aaagggaagt
     3541 atgattacta gagttgcnnn nnnnnnnact tagattctga aactttttgg ttatataaaa
     3601 atgcgcacta tattgatcaa gagtggattg ttaaagcagc gggaattagg cagcgtcatc
     3661 tcgaccagtc acaatcagtt aatttatata tgacaaatga gtttactttt agaaagttgc
     3721 ttaatttgta tattaaagct tgggagtatg gtgttaaaac attatattat gttagaagcc
     3781 agtctttaga agttgaagaa tgtgaatcat gcagttcata ggaggtaata atggaactta
     3841 aacaaaaagc attatttaat gctaaaggtg atattgatgt aagaaaacgt cgaatgataa
     3901 atggaaatac aacaaatctt aatgatttta ataatatgaa atatcagtgg gtcagtgatt
     3961 ggtaccgtca ggcgatgaat aatttttgga ttccagaaga aataaatttg actcaagata
     4021 ttagagatta tcgtttgtta agtgaggcag aacgaaccgc atatgataag attctttcat
     4081 ttttagtttt cttagattct ttacagacag ccaatcttcc taatattggt gagtatatta
     4141 cagcaaatga aattaatctt tgtttaacca ttcaagcatt tcaagaagcg gtccattcac
     4201 aaagttatag ctatatgctt gattcaattt gttcnnnnnn nnnnactaaa cgagatgaaa
     4261 ttttatatca atggaaattt gatgaacatt tgttgaagcg aaatgaattt attggtgaac
     4321 agtataatgc atttcttgaa cataaagata gttttcattt aatgaaaacg attatggcta
     4381 attatatttt ggaaggaatt tatttttatt caggatttat gttcttttat aatttagggc
     4441 gaatgggtaa gatgtcggga tcagctcaag agattcgtta t
//
LOCUS       DAOMTA010000162         4478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_550_length_4478_cov_2.037079, whole genome shotgun sequence.
ACCESSION   DAOMTA010000162 DAOMTA010000000
VERSION     DAOMTA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_4478_cov_2.037079"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLK68_05710"
     CDS             complement(<1..508)
                     /locus_tag="PLK68_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM89942.1"
                     /translation="MLARHTKILELITENKKMEVTKLSQLLSVSQVTIRKDLIQLENS
                     GLIVREHGFATLNSSDDINNRLAYHYDIKQRIAKLAVESIEDGETVMIESGSCCALVA
                     LEIAQTKKDVTIITNSAFIADYIRKVAKIRIILLGGEYQNESQVMVGPITKKCVESFF
                     VDKLFIGTD"
     gene            complement(626..1288)
                     /locus_tag="PLK68_05715"
     CDS             complement(626..1288)
                     /locus_tag="PLK68_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM89943.1"
                     /translation="MEVTEYAKDLYILDDGQVREFLIIGRNEAILIDTGFPETKIINE
                     VQKLTSFPLKVLLTHGDGDHCGGLDSFQECYVHQGDKGLIKTTIKINCLKEKDLIEIG
                     DYCFEVIEIPGHTYGSIAFLDIQKKLLIVGDTVQLGPIYMFGEHRNLDLYIHSLEKLN
                     TYQNQIETILPSHNRYPLTKEYIGHCLSDARLLKAHKLLGVKHQFLPCREYRGKKISF
                     YY"
     gene            complement(1360..3576)
                     /locus_tag="PLK68_05720"
     CDS             complement(1360..3576)
                     /locus_tag="PLK68_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRM89944.1"
                     /translation="MEKQKMVFESGNELAAYAAKQINYHVMGYYPIKPSTQIAENLDL
                     MKAEGKHEIALIAAEGEHSAAGICYGASVAGARVFNATSANGLLYALEQFPVQSGTRF
                     PMVMNVACRTVSGPLSIKGDHSDIMYMLNTGWIILFAHSPQAVYDMNICALKIAEKVK
                     LPVVIAFDGFFTSHQKNKCQVFEDDQVVQNFVGKYLPEYQILDFEHPVTVGSYMNEPD
                     LMNNKYQLHLAMEEAREVIPAIFKEYETISNRAYQYVESYQNEDCDVLMFVLGSGFSS
                     AKRAVDELRKDDKKVGVVTINVLRPFPSKELIKHFKVPKTVIVCDRQDSYGANGGNMS
                     LEIKAAMQEAGITTRVITRIYGLGGRDFYKDDAKALLLMGFQKDVKLFDYLHIYPGKI
                     EQPITQFFKPIKETPDDFKCVYNEEKQIMEVKPFTLNQIAKMPQRLSGGHGACPGCGI
                     PVNVNLLLSAISGNVVLLFQTGCGMVVTTSYPKTSFKVPYVHNLFQNGAATLSGIVEA
                     FNQKVKRHEYPEGEITFIMVSGDGGMDIGMGSALGAALRNHHMIIFEYDNGGYMNTGY
                     QLSYSTPLGAKSSTSHLGQDQFGKSFFNKDMPAIMAATNIPYIATVAESNPIDFVRKA
                     IKAKAYAKEFGLAYLRTLSACPLNWGDQPNLEKSVIEAGVNSCYFPLFEIEQGKYNLT
                     YDPQKAKKKIPLIDWFAMMQRTKHLSDPKYTKIVQAAQDEVDRRFEILKKRADDQI"
     gene            complement(3551..>4478)
                     /locus_tag="PLK68_05725"
     CDS             complement(3551..>4478)
                     /locus_tag="PLK68_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRM89945.1"
                     /translation="FIRYCDEDKEITVNTPVTRPHLLGLFHEAMIGKLQVTAGVDENS
                     CIVINSGEQPDVIRDKLKLCAGTIYCIDAIYLAMKIKCKVNMIMLGALAKASGFIPLE
                     TVLELIEATLGKKYPQLLQKNKEGIIEGYNGVVSKTFNDDGKYPFIPYREIENDWGYQ
                     NAPLGGVNPCFGSTISNDLSASREGYIPLFLQEKCIQCAQCYITCPDMVFQFEPGIYK
                     GRKMMVNKGLDYHHCKGCLRCVEICPTQALVSGVEREHSNLKWFIRNKDLIVEHMDYE
                     DVGANSWITSDSFLEVEKITEVKSDGKTKDGI"
BASE COUNT         1465 a          930 c          550 g         1533 t
ORIGIN      
        1 catcagttcc aataaaaagt ttatcaacaa aaaaagattc aacacatttt tttgtgatcg
       61 gtcctaccat tacttgtgat tcattttgat attcaccacc aagtaaaata attctaattt
      121 tagcaacttt acgaatataa tcggcgataa aagcagaatt agtaataatc gtaacatctt
      181 ttttggtctg cgcaatttct aaagcaacca atgcgcaaca agaacctgac tcaatcatta
      241 cggtttcacc atcttctatt gattctaccg ctaatttagc aattctttgc ttaatatcat
      301 aatgataagc taaacgatta tttatatcat cactagaatt taaagttgca aatccatgct
      361 cacgaacaat caatccacta ttttccaatt gaattaaatc ttttctaatt gttacttgtg
      421 aaacactgag taactgagaa agtttagtta cttccatttt cttattctct gttattaatt
      481 ctagtatttt tgtatgtctt gccaacatct taaccatcct catcaccgct cttttctttt
      541 attaccttat catattaata tttcaaatac aataaaaaca actttcgttt caatcatatt
      601 attctcaaaa cgaaagttat tatttctaat aatagaacga aattttttta ccacgatatt
      661 cccgacatgg taaaaattga tgtttaacac ctaaaagttt atgtgccttt aacaatctag
      721 catctgatag acaatgacca atatactcct tagttaatgg ataacgatta tgactaggca
      781 aaattgtttc aatttgattt tgatatgtat ttaatttttc taagctatga atatacaaat
      841 ctaaatttcg atgctcacca aacatataaa tcggtcctaa ttgtacagta tcaccaacaa
      901 ttaataattt cttctgaata tctaaaaatg caatacttcc ataagtatgt ccaggtatct
      961 caattacctc aaaacagtaa tcaccaattt caatcaaatc cttttctttt aaacaattaa
     1021 tttttattgt tgttttaatt aaacccttat ctccctgatg tacataacat tcttgaaaac
     1081 tatcaagtcc cccacaatga tctccatcac catgagttaa cagtacttta agcggaaaac
     1141 tagtaagttt ctgaacttca ttaattatct tcgtctcagg aaaaccagta tcaatcaata
     1201 tcgcttcatt tctaccgata attagaaatt ctcgtacctg accatcatct aaaatatata
     1261 aatctttagc atattcagtt acttccacaa ttaatcacct tcttttactc attataaaag
     1321 gttattgaca caacaagcaa taacctttta tattatttat taaatttgat catctgccct
     1381 ctttttcaaa atttcaaaac gtcgatcaac ttcatcttga gcagcctgaa caatttttgt
     1441 atactttgga tcacttaaat gtttagttcg ctgcatcatt gcaaaccaat caattaatgg
     1501 aatctttttc tttgctttct gtggatcata tgtaagatta tacttaccct gctcaatttc
     1561 aaacaaagga aaataacaac tatttacccc tgcttcaatt acactttttt ctaaatttgg
     1621 ctgatcaccc caatttaacg gacaagcaga taaagttctc aaatatgcta acccaaactc
     1681 tttagcataa gccttagctt taatcgcttt tctaacaaaa tctattggat tagattcagc
     1741 tacagtagca atatatggaa tatttgttgc agccatgatt gctggcatat ctttattaaa
     1801 aaatgatttt ccaaactgat cttgaccaag atgagaagtc gaacttttag cccctaatgg
     1861 tgttgaatat gataattgat aacctgtatt catataacca ccattatcat attcaaaaat
     1921 aatcatgtga tggtttctta aagctgcacc tagtgcactt cccattccaa tatccattcc
     1981 tccatcacca ctaaccatga taaaagtaat ttctccttcg ggatattcat gtcgttttac
     2041 tttttggtta aacgcttcta cgattcctga aagtgtcgct gccccatttt gaaataaatt
     2101 atgaacatac ggtactttaa aagaagtttt tggataggaa gttgttacaa ccattccgca
     2161 gcctgtttga aataataaaa caacatttcc actgattgca cttaataata aattgacatt
     2221 gacgggaatc ccacatcctg gacaagcacc atgaccccca gataaacgtt ggggcatctt
     2281 tgcaatttga ttaagcgtaa acggcttaac ttccattatt tgtttttctt cattataaac
     2341 acacttgaag tcatcaggag tttctttgat tggtttaaaa aattgagtta ttggttgttc
     2401 aattttaccg ggataaatat gtaaataatc aaataatttt acatcttttt gaaatcccat
     2461 taataataat gcttttgcat catctttata aaaatcacgg ccacctaagc cataaattct
     2521 cgtaattaca cgggtagtga ttcctgcctc ctgcattgcc gcctttattt ctaaagacat
     2581 atttccacca ttggcaccat aactatcctg acgatcacaa acaatcactg ttttaggcac
     2641 tttaaaatgc ttaattaatt ccttacttgg aaatggacgc agtacattga ttgtaactac
     2701 tccaactttt ttatcatctt tgcgtaattc atcaacagct cgttttgcac tgctaaatcc
     2761 agaacctaga acaaacatta agacatcaca atcttcattt tgataacttt ctacatactg
     2821 ataagcccga ttagaaattg tttcatattc cttaaaaata gccggaataa cctctcgagc
     2881 ttcctccatt gcaagatgaa gctggtattt attattcatt aaatccggtt cattcatata
     2941 agagccaact gtgactggat gttcaaaatc aagaatttga tattcaggca gatatttacc
     3001 tacaaaattt tggacaactt gatcatcttc aaagacttga catttatttt tttgatggct
     3061 agtaaaaaaa ccgtcaaacg caataactac tggtaattta actttttcag caatttttag
     3121 agcacaaatg ttcatatcat aaactgcctg gggactatga gcaaataaaa taatccatcc
     3181 agtatttaac atatacatga tatcgctgtg atcacccttg attgaaagtg gaccagaaac
     3241 tgttcgacaa gctacattca ttaccatcgg aaatcgtgtt ccagattgaa ctggaaactg
     3301 ttctaatgca tataacaaac cattggcact agtcgcattg aaaactcttg ccccagcaac
     3361 gcttgcacca taacaaattc cagccgcact atgctcacct tcagcagcaa ttaaagcaat
     3421 ctcatgctta ccctcagctt tcattaaatc taaattctca gcaatctgcg ttgatggttt
     3481 aatgggatag tatcccatta catgataatt aatctgttta gcggcataag ctgccaattc
     3541 attcccactt tcaaatacca tcttttgttt ttccatcact ttttacctcc gttatttttt
     3601 ctacctccaa aaacgaatca ctcgttatcc acgaatttgc tcccacatct tcataatcca
     3661 tatgctcaac aattaaatct ttgttacgaa taaaccattt taaatttgaa tgttcgcgtt
     3721 cgactccact aaccagtgct tgagttggac aaatttcaac acaccgtaaa catcctttac
     3781 aatgatgata atccaaacct ttatttacca tcatcttacg tcctttataa atacccggtt
     3841 caaactgaaa aaccatatcc ggacaagtaa tataacattg tgcacattga atacattttt
     3901 cttggagaaa caatggtata tagccttccc gtgatgcact taaatcattc gaaatagtac
     3961 ttccaaaaca aggattcaca ccaccaagtg gtgcattttg ataaccccag tcattttcta
     4021 tttcacgata aggaatgaac ggatacttac catcatcatt aaacgtttta ctaacaacac
     4081 cattataacc ttcaattatc ccttctttat ttttttgtaa tagctgagga tattttttac
     4141 ccaaagtagc ttcaatcaat tctaaaactg tctctaaagg aataaagcca ctcgctttag
     4201 ctagtgcccc cagcatgatc atattgactt tacattttat tttcattgct aaataaattg
     4261 catcaataca atatatcgtt ccagcacata attttaattt atctctaatt acatctggtt
     4321 gctcaccgct gttgatgaca atacaactat tttcatcgac tcctgctgtt acttgtaact
     4381 ttcctatcat cgcttcatga aaaagcccaa gcaaatgggg tcgcgtaacg ggagtattaa
     4441 cagtaatttc tttatcttca tcacaataac gaataaac
//
LOCUS       DAOMTA010000163         4476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_551_length_4476_cov_2.224836, whole genome shotgun sequence.
ACCESSION   DAOMTA010000163 DAOMTA010000000
VERSION     DAOMTA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_551_length_4476_cov_2.224836"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLK68_05730"
     CDS             <1..441
                     /locus_tag="PLK68_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HRM89946.1"
                     /translation="GNKFFLLLSFFAFLVWFEIIYGIFFRDDVKKRRLTKNEQLKYAK
                     IDRTRDVKKSLLRVDFNKTGLVEHRMSKQALKIKMTPIIDKINKRTKDIPILNNTQKV
                     LIPAKCWNIGDKKCYVRAGFPITGNRKCIYVDPGDVHNLVIGTT"
     gene            490..1875
                     /locus_tag="PLK68_05735"
     CDS             490..1875
                     /locus_tag="PLK68_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM89947.1"
                     /translation="MCGESVVVNDPKGELAKHTLQKFKDNGFNTVVINFVDPEFSNCW
                     NPFKLAFDEWKKADEIYIKKYEEWKIKNKNGILEYELLGLDTKEIENKLRKPKPNYSK
                     AMDLLRDACNTICLEENPKDPIWTETARDMLVGCGALMCEEGKVEYMNATSFKKIIEI
                     GKKQLPGIRNSKTILKAFLDSERSITDYSVNYLEGYVGSSGDTSTGFESQFNNKLSIL
                     TANEDIQRITSHSDFDFRQIGSEKTAIFLIVHDEKKTYYPLVTLFIKQLYEVLVRTAR
                     DEINLRLKVPINLILDEFGNMPALPDVDAMLTAARSRGIRLTAIIQGLEQLEKHYGKM
                     AETLKGNFTNTIYLLSGSDSTVEEISKKAGSERVWNKDMRKYEDQRIFTPERLKDFKM
                     GEILFLRQRHKNPYYTRLLPYDKYVFFDKSKFYLNLPIVEKPDVELFNIEYEYLKRHG
                     KADKFDRFARN"
     gene            1889..3313
                     /locus_tag="PLK68_05740"
     CDS             1889..3313
                     /locus_tag="PLK68_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89948.1"
                     /translation="MSWDEKEQAKKIREEYNIKYNNYMTLNLEQLNLCEDKETNASDF
                     ECESRTFADRIIVGKDRKRFAVKNSYFEYINFRKFECEETVFINCFFKGVKFIDCDVP
                     NIKFINCNFDDFEIVRSNFIGSIFSGCLFIRSRVEESTLDESIFPLSNYYDIEIDTDK
                     SKPNYSTVKGVLASEQKEVSSIETILKSDEVVPIEVETIENKIYKHETIVIEELKNNK
                     FVKCEFIECVFENDKETDNYINDLSIEFWNSNFKKCLFNATFFKTVFDGSNFLECRFK
                     DKYIRCSFCDTVWNKNILEDTAVFNYSMIINAVLDFDFTSIPERNRKNVGIGTTANSK
                     NIVNNLSEKDRQIEQLNKENKLLKEELEKLDCKKKNLISFEEFDDQLLNEAFRLIIAR
                     CQRPEPVEFDLWNEIKAHIDSLSDLDFIQFVNSINNYKLSSNNLIDEDRQEKNMDNPD
                     NNYIHEVAQEEKKEKLPGLEDGVV"
     gene            3316..4401
                     /locus_tag="PLK68_05745"
     CDS             3316..4401
                     /locus_tag="PLK68_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM89949.1"
                     /translation="MTKLEDLKHLAFSPLQMNVLRKAEKDGLNLEWLANTNLDYLQME
                     EIYLCLKYDVDPSSISDPDIPAESMRSLREHLFEMQGVYEDERIKINKQKTKLIILIV
                     SFVLVLSSLIAVVYINRDYISLYFEDINLTLKADKVEIGMSKSFNPSDYIKEYDKRYD
                     LTLPKNKKFEVPGEYTVIYKLSNKVKSIKKKLIVKVIDDIEPVLVLNKTNLTLEYGQN
                     IDLKDYIEKAEDNIDGNLVEKVDYNQIDTSKPGNHVVTYSVKDKAGNHVSAEMSINIK
                     EKPKVTEKSTNSNTENNVTTNKSNNSNNSKSGQENIPSQFDKFFSGNSIDVYNQANSY
                     AEEIFSSGKVKGFSVNPTGEGFQVSFS"
BASE COUNT         1737 a          490 c          849 g         1400 t
ORIGIN      
        1 ggtaacaagt tttttttatt attatctttt tttgcttttt tagtatggtt tgaaattatt
       61 tacggaatat tttttagaga tgatgtcaaa aaaagacgac ttacaaaaaa tgaacagctt
      121 aaatacgcta aaattgatag aacaagagat gtaaagaaat ctttactaag agttgatttt
      181 aataaaactg gacttgttga acatagaatg tcaaaacaag cattaaaaat caaaatgaca
      241 ccaattattg ataaaataaa taaaaggaca aaagatatcc ctatcttaaa taatacacaa
      301 aaggtgttga ttcctgctaa atgttggaat attggtgata agaaatgtta tgtacgtgct
      361 ggatttccaa ttacaggaaa tagaaaatgt atttatgttg atccaggtga tgttcataat
      421 ttggttatag gtactacttg atcaggaaaa acatggactt ttgtaaatga atatttagag
      481 ttgtgtgcta tgtgtggaga aagtgtagtc gtgaatgatc ctaagggaga actcgcaaaa
      541 cataccttac agaaatttaa agataatggg ttcaacactg ttgtaataaa ttttgttgat
      601 cctgaattta gcaactgctg gaatccattt aaacttgcat ttgatgaatg gaaaaaagca
      661 gatgaaatat atataaaaaa atatgaagaa tggaaaatta aaaataaaaa tggaatatta
      721 gagtatgaat tgcttgggct ggatacaaaa gaaattgaaa ataaattaag aaaacccaag
      781 ccaaattatt ctaaagcaat ggacttatta agagatgcct gcaatacaat ctgtttagag
      841 gaaaatccta aagatccaat ctggactgaa acggctaggg atatgcttgt tggctgcggt
      901 gccctaatgt gcgaagaggg taaggtggag tacatgaatg cgacttcatt caaaaaaatt
      961 attgaaattg gaaagaagca gctgccaggt ataagaaatt ctaaaacaat actgaaggct
     1021 tttcttgact cggaaagaag tataaccgac tacagtgtta attatctgga agggtatgta
     1081 ggatcaagtg gagatacatc gactggattt gaatctcagt ttaacaacaa gttgagtatt
     1141 ttgacagcta atgaagatat tcaaagaata acaagtcata gtgactttga ttttcgtcaa
     1201 atcggtagtg aaaagactgc tattttcctg attgttcatg atgaaaagaa aacatactat
     1261 cctttagtta cactgtttat caaacaacta tatgaagtgc ttgtacgtac tgcaagagat
     1321 gagattaatc taagattaaa agtacctatt aatttgattt tagatgaatt tggtaatatg
     1381 ccagcattac ctgatgttga tgcaatgttg acagctgcta gatcacgtgg tattagacta
     1441 acagcaatta ttcaaggact agagcaactg gaaaaacatt atggaaaaat ggcggaaaca
     1501 cttaaaggta attttacaaa tacaatttat ctactatctg gttctgatag cacggttgaa
     1561 gaaatatcta aaaaggcagg cagtgagcgt gtttggaata aggatatgag aaaatatgaa
     1621 gatcaacgta tttttacccc ggaacgtctt aaggatttta aaatgggaga aattttgttt
     1681 cttaggcaga gacataagaa tccttactac actaggttac ttccgtatga taaatacgta
     1741 ttttttgata agagtaagtt ttatttgaat ttacctatag ttgaaaagcc agatgtagaa
     1801 ttatttaaca ttgaatatga atatctaaaa agacatggta aagcagataa atttgataga
     1861 tttgcaagaa attaaggagt gagtaaaaat gagctgggat gaaaaagaac aagctaaaaa
     1921 aattagagaa gaatataata taaaatacaa taattatatg actttaaacc tggaacaatt
     1981 aaatctatgt gaagataaag aaactaatgc aagtgacttt gagtgtgagt caagaacatt
     2041 tgctgatcgc ataattgtag ggaaagatag aaaaagattt gcggtcaaga atagttattt
     2101 tgaatacatt aattttagaa agtttgaatg tgaagaaaca gtatttatta attgtttttt
     2161 caagggagtt aaatttatag attgtgatgt accaaatatt aaatttatta attgtaattt
     2221 tgatgacttt gagattgtaa gatctaattt tattggaagt atattttctg ggtgcttatt
     2281 cattagaagt agggtcgaag aaagtacttt ggatgagagt atatttccgc tttcaaatta
     2341 ttacgatata gaaattgata cagataagag caagcctaat tattcgactg ttaaaggagt
     2401 attggctagt gaacaaaaag aggttagcag tatagaaaca attttaaaat cggatgaagt
     2461 agtgccgatt gaagtagaaa ccattgaaaa taagatatat aagcatgaaa cgattgttat
     2521 tgaggaatta aaaaataata aatttgtaaa atgtgagttt atcgaatgtg tttttgaaaa
     2581 cgataaagag actgataatt atattaatga tttatcaatt gaattttgga attctaattt
     2641 taaaaaatgt ctatttaacg caactttttt taagaccgtt tttgatggct ctaattttct
     2701 agaatgtaga tttaaagaca agtatataag atgtagtttt tgtgatactg tatggaataa
     2761 aaatattctt gaagatacag cagttttcaa ttattcgatg attattaatg cggtattgga
     2821 ttttgatttt acatctatac ccgaaagaaa tagaaaaaat gttggaatag gtacaacagc
     2881 aaatagcaaa aatattgtta ataatctatc agagaaagat agacaaattg agcagctgaa
     2941 caaagaaaat aagctattaa aggaagagct ggaaaagtta gattgtaaaa agaaaaattt
     3001 aatttcattt gaagagtttg atgatcaact gttaaatgaa gcctttagac tgattattgc
     3061 acgttgtcag aggccggagc ctgtagaatt tgatttatgg aatgagatta aggcacacat
     3121 tgacagttta tcagatttgg attttataca gtttgttaat agtatcaata actataagtt
     3181 gtcatcaaat aatcttatag atgaggatag acaagaaaaa aatatggata accctgataa
     3241 caattatatt catgaggtcg ctcaagaaga aaagaaagaa aaattaccag gtttagaaga
     3301 tggagtggtt taatgatgac taagttagaa gatttgaaac atcttgcttt ttcaccacta
     3361 caaatgaatg ttttacggaa ggcagaaaaa gacggattaa atcttgagtg gttggctaat
     3421 acaaatttag attatcttca aatggaagaa atttatttat gcttaaaata tgatgtagat
     3481 ccatcttcaa tatctgaccc tgatatacca gctgagtcga tgagatcttt gcgtgaacat
     3541 ctgtttgaaa tgcagggcgt ttatgaggat gaaagaatca aaatcaataa acaaaaaaca
     3601 aagctcataa ttttaattgt gagttttgtt ttagtacttt cttcactgat agcagttgta
     3661 tatattaaca gggattatat atcattgtac tttgaagata ttaacctgac tttaaaagct
     3721 gataaagttg aaatagggat gtcaaaatct tttaatccta gtgattacat aaaagagtat
     3781 gataaaaggt atgacttaac actcccaaag aataaaaaat ttgaagtacc tggggaatac
     3841 acggttattt ataaactctc taataaagta aaatccataa aaaagaagct gattgtgaaa
     3901 gttattgatg atattgaacc agtcttagtg ttaaataaaa cgaatttaac attagagtat
     3961 ggtcaaaata ttgatcttaa agactatatt gaaaaggcag aagacaatat agatggaaat
     4021 cttgtagaaa aagttgatta taatcaaatt gatacttcta agcccggtaa tcatgttgtt
     4081 acttatagtg taaaagacaa agctggtaat catgtttcgg cagagatgag tatcaatata
     4141 aaagaaaagc caaaagttac tgaaaaatct acaaacagta atacagaaaa taatgttact
     4201 actaataaat ctaataattc aaataattca aagtcaggac aggaaaatat tccttcacaa
     4261 tttgataaat ttttttcagg taatagtata gatgtgtata atcaagcgaa ttcctatgct
     4321 gaagagattt ttagtagtgg aaaggtaaaa ggattttctg tgaacccaac aggtgaaggg
     4381 tttcaagtca gtttttcata atatgaggag gtaagaagaa tggcgttgtt tgaaacagtt
     4441 agagatatta tgtttgcaaa aattatggaa gccaca
//
LOCUS       DAOMTA010000164         4452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_558_length_4452_cov_2.394815, whole genome shotgun sequence.
ACCESSION   DAOMTA010000164 DAOMTA010000000
VERSION     DAOMTA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_558_length_4452_cov_2.394815"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..556)
                     /locus_tag="PLK68_05750"
     CDS             complement(257..556)
                     /locus_tag="PLK68_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89950.1"
                     /translation="MNIYYIIGAVAIILILLFVYWYSHRSNKEPHEYILVVSFDDGYY
                     YDLCEDERFKKLAQFEKNNNEFIAKTKLNEVYLKKEIGKILEMDLAKLHVIIKRW"
     gene            complement(786..1175)
                     /locus_tag="PLK68_05755"
     CDS             complement(786..1175)
                     /locus_tag="PLK68_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89951.1"
                     /translation="MTIAEVSKKFGISLTTLRYYEKIGLMNPVAKNISGHRDYQEPDL
                     RRINFIKCMRAAGMTIEQIKLYVDLFNEGEHTISQRKDIMIEQLGNLEAQVEELQSII
                     SYLKHKIDNYESILVKREMEQRNKLKG"
     gene            complement(1229..2383)
                     /locus_tag="PLK68_05760"
     CDS             complement(1229..2383)
                     /locus_tag="PLK68_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM89952.1"
                     /translation="MKEKLWTKKYLLSVIILFAICLCSNIVLSVLTIFAKNLTGLDTY
                     AGLMTSIFTLAALCVRFIAGILLDKFGCKKVILGGITLMMAAAFFFIDCKQIELAIIY
                     RIMQGIGFGIASTGASTYVAKMCHPNRLLEGVSYASIANSLTGVVGPSIAYSILGANY
                     DRFKLLFIVSLLICILTFGLMLLGKDVSIVSIENQNKEKETINWLILIVPILVLFFNS
                     LTQSAITSFVSLYAISLEFAGAGMFFSVNAIGMISSRFIMNRLVIHFGEFKMLLLNSL
                     LFFISVYLIGQVTRMYQLLFLALPAGFATGAVAPIVNTFLIKRMPESKNGIANATYYA
                     AMDIGYAIGSVVWGIIAAFSGYRMIFYLGALMQIVGVILCLAQMKIYRLK"
     gene            complement(2386..3399)
                     /locus_tag="PLK68_05765"
     CDS             complement(2386..3399)
                     /locus_tag="PLK68_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM89953.1"
                     /translation="MEIRKLGSNFEISAIGLGCMGMTHGFGDASDQKEMIEVIRGAYQ
                     AGITMFDTAECYQGVSETGEILYNEDLVGQALSIYPRDSYQIATKCGIKVIDGKQVLD
                     ARPEVIRTSLSESLKRLKTDYVDLYYLHRVDPKTPIELVAQTMKELMAEGKIKHWGLS
                     EAGVETIRKAHAICPLTVVESEYSMIFREPEKNGLLDTLKELKIGFIPFAPLGKGFLT
                     ATIDPNQTFSKNDTRSKQPRFKKENMAINQVLVELIKKLAKEKQVTPAQIALAWVMAQ
                     GDWIVPIPGSRKLSRIEENIKASKVILDEVDLKNIKRALDNMDLKAERWDPNSDNAKR
                     VGK"
     gene            3659..>4452
                     /locus_tag="PLK68_05770"
     CDS             3659..>4452
                     /locus_tag="PLK68_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM89954.1"
                     /translation="MKAFSQMNNNELCELKKELEKRYLDFKARDLKLDMSRGKPASEQ
                     LDLSLGLLKQEDYMIDGIDCRNYGGLEGLPAMRRFFGELLDVNEKNVIVGGTSSLTLM
                     YDYLSQAMLFGVMGNTPWSKLDKVKFLCPVPGYDRHFAFCEHFGIEMINIPMNSDGPD
                     IETIKKHLQDESVKGMFCVPKYSNPQGITYSDDIIKAIASLTPAAKDFRIIYDNAYCV
                     HDLNEHGDTLLNIFNELPKYQHEDMVIMVASTSKISFAGAGVSCIVA"
BASE COUNT         1546 a          821 c          563 g         1522 t
ORIGIN      
        1 tcttaaccgt ttacggatac ataaaagtcc taaaatcatc aaaagagccg aaatataggc
       61 aggaattaaa tttacatcat tgatcgttaa tttaagacca tacataaaaa taaatgctaa
      121 tgcgattaaa ccaatcccca taattattac ctctctctac ttcaagtata tctaataatt
      181 tagattgttt catgcctttt ggtctaaatt gcattaaatt agcctgaatt acaaaaaatc
      241 acagtaaata ctgtgattac caacgtttta taattacatg cagcttagct agatccatct
      301 ctaaaatttt accaatttcc ttttttaaat aaacttcatt taattttgtc ttggcaataa
      361 attcgttatt attcttttca aattgagcta attttttaaa tctctcatct tcacataaat
      421 cataataata gccatcgtca aatgatacaa ctaaaatata ctcatgtggt tctttattac
      481 tacggtgact ataccaatat acaaataata agattaaaat gattgcaacc gccccaataa
      541 tataataaat attcattctt ctacctccga ctatatcaat ttaactaaaa aaactttacc
      601 ttattattag taacaaattt gactttgaat aattttttta cttaatcatc taaattataa
      661 cattttataa aaagaaaatc ccgtagaata ataacattct acgagatctg ggaagtaatt
      721 catttactat ttatattcta tcatatgata agtatttttt ctaccaatcc agctttaaaa
      781 acaaactacc cttttagttt attccgctgc tccatttctc tttttactag aatactttca
      841 taattatcta ttttatgctt taaatagctg atgatcgact gtaattcttc aacttgtgct
      901 tctaaatttc ctaattgttc gatcataata tcttttcttt gagaaatcgt atgctctcct
      961 tcattaaata aatctacata caactttatt tgctcaattg tcattccagc agcccgcata
     1021 catttaataa aattgattcg acgcaaatcc ggttcctgat aatctcgatg accactaata
     1081 ttttttgcta caggattcat taatccaatt ttttcataat atcgtaatgt tgtcaaacta
     1141 atcccaaatt ttttactaac ttctgcaatt gtcattttca tcaccctctt tatataatta
     1201 taagtcataa tttgtagacc ttcaattttt attttaaccg ataaatcttc atttgagcta
     1261 aacaaagaat gacaccaact atttgcatta atgccccaag ataaaaaatc atccgataac
     1321 cactaaaggc agcaataatt ccccaaacaa ctgaaccaat tgcataccca atatccattg
     1381 cagcataata tgtcgcatta gcaataccgt ttttactttc cggcattcgc ttaattaaaa
     1441 atgtattaac aatcggggca acagctccag ttgcaaatcc agcaggaagc gctaaaaaca
     1501 ggagttggta catcctagtt acttgaccaa ttaaatatac actaataaaa aataataaag
     1561 aatttaataa caacattttg aactcaccaa aatgaataac tagtctattc ataataaaac
     1621 gcgaactaat cattccaatt gcatttactg aaaaaaacat tcctgctcca gcaaattcaa
     1681 gactaattgc atatagagaa acaaatgagg taattgcact ttgcgtcaag gaattaaaga
     1741 ataatactaa aatcggaaca attaaaataa gccaattaat agtttctttc tctttatttt
     1801 gattttctat gctaacaatt gatacatctt tacccagcag cattaaccca aaagttaaaa
     1861 tacaaattaa taatgaaaca ataaaaagca atttaaagcg atcataatta gctcctaaaa
     1921 tactataagc aatcgatggt cctactaccc ctgttaaact gttggcaata gaagcataac
     1981 taaccccttc taataatcga ttaggatgac acattttggc aacatatgta ctagccccag
     2041 ttgaagctat tccaaaccca atcccttgca ttattcgata aataatcgcc aattcaattt
     2101 gcttacagtc aataaagaaa aatgctgcag ccatcatcaa cgttatacca cctaatatta
     2161 cttttttaca cccaaattta tctaacaaaa tacccgcaat aaaacgaaca cacaaagctg
     2221 ccaaagtaaa aattgatgtc atcaatcctg cgtatgtatc taatccagtt aaatttttag
     2281 caaaaatcgt tagaaccgac aatacaatat tggaacaaag gcatattgcg aaaagaatta
     2341 ttactgataa tagatacttt tttgtccaaa gtttttcttt cacgactatt ttcctactcg
     2401 tttagcatta tcagaatttg gatcccaacg ttctgccttt aaatccatat tatctaatgc
     2461 tctttttata tttttcaaat caacctcatc taaaataact ttgctggctt taatattttc
     2521 ttcaatccga cttagctttc gacttcctgg aataggtacg atccaatcac cttgagccat
     2581 tacccaagct aaagcgattt gagctggagt tacctgtttt tcttttgcta acttcttgat
     2641 caattcaact agtacttgat ttattgccat attctctttt ttaaatcgtg gttgtttact
     2701 tctagtatca tttttagaaa aagtttgatt agggtcgatc gttgctgtta aaaatccttt
     2761 acctaatggt gcaaatggaa taaatcctat tttcaattcc tttaatgtat caagtaatcc
     2821 atttttttca ggttctctaa aaatcatcga atactcactc tcaactactg taagtgggca
     2881 aatagcatga gcttttctta ttgtttcaac gcctgcttct gataatcccc aatgctttat
     2941 ttttccttcc gccatcaatt ctttcattgt ttgagcaaca agctcaatcg gtgttttagg
     3001 atcaacacga tgtaaataat ataaatccac ataatctgtt tttaaacgtt tcagtgattc
     3061 tgataaagaa gtgcgaatta cttcaggacg tgcatctaaa acctgctttc catcaatcac
     3121 cttgattcca cacttagttg cgatctgata actatcacgg ggatagatac tcaatgcctg
     3181 tccaactaaa tcttcattat ataaaatttc tcctgtttca ctaacacctt gataacactc
     3241 tgcagtatca aacattgtaa ttcctgcttg ataagcaccg cgaattactt caatcatttc
     3301 tttttgatca ctcgcatcac caaaaccatg ggtcattccc atacatccta gaccaattgc
     3361 tgatatttca aaattactac cgagttttct tatttccatt ttctcttcct ccaatatctt
     3421 tgataaatac atcctaatac ttagagtcaa ctataagtca atagttttaa ctaaaaaaga
     3481 acaactccct tattcataaa attaatagat tgtttttcgt aaaatacatt attttactcg
     3541 ttttactaaa tagatataaa tgttgataaa tttaagcaaa taatcttgaa aaattaacat
     3601 ataatggtac aatacatacg gtcttaaata tacaagctga ttacaggggg aaaagccaat
     3661 gaaagcattt tcacaaatga acaataatga gttatgtgaa ttaaaaaaag aattagaaaa
     3721 aagatatctt gatttcaaag caagagattt aaaattagat atgtcacgcg gtaaaccagc
     3781 tagtgaacaa ttagatttat cactcggttt attaaaacaa gaagattata tgattgatgg
     3841 cattgattgc cgtaattatg gtggccttga aggtttacca gcaatgcgaa gattctttgg
     3901 tgaattatta gatgtaaatg agaaaaatgt tattgtaggt ggaacgtcta gtctaacatt
     3961 gatgtatgac tatcttagtc aagctatgtt attcggtgtc atgggaaata ctccatggtc
     4021 aaagttagat aaagtaaaat ttttatgtcc agttcctgga tatgaccgtc actttgcttt
     4081 ttgtgaacat ttcggaattg agatgatcaa tattccgatg aatagtgatg gtcctgatat
     4141 tgagacaata aaaaaacatc ttcaagatga aagtgttaag ggaatgttct gtgttcctaa
     4201 atattctaat cctcaaggaa tcacatatag tgatgatatt attaaagcaa ttgccagttt
     4261 aacaccagca gcaaaagatt ttagaattat atatgataat gcttactgtg ttcatgattt
     4321 gaatgagcat ggcgataccc ttttaaatat atttaacgaa ttgcctaaat atcagcatga
     4381 agatatggtc atcatggtcg cttcaacatc aaaaatcagt tttgctggag cgggtgtatc
     4441 atgtattgtt gc
//
LOCUS       DAOMTA010000165         4444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_560_length_4444_cov_2.143313, whole genome shotgun sequence.
ACCESSION   DAOMTA010000165 DAOMTA010000000
VERSION     DAOMTA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_4444_cov_2.143313"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLK68_05775"
     CDS             complement(<1..621)
                     /locus_tag="PLK68_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM89955.1"
                     /translation="MLLINKTLIRMSEGIRGWIAIITGLKILTLVGTVMFAKTISSFL
                     SDLYHPKMSQEQLLAAIISALIASCLILIADLLTGEAEYRCGAKARINLRKEIFEKML
                     QLDVGKIERIGASNTISSVGDGVELMQVYYTKYLPSLLYCFFAPVYLFFQLKDTSLLV
                     ATILLIISLSLPLVNNYFRKMIDQLKKEYWTSFQDLTSYYLESLKSL"
     gene            complement(809..1084)
                     /locus_tag="PLK68_05780"
     CDS             complement(809..1084)
                     /locus_tag="PLK68_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89956.1"
                     /translation="MDQRVKKAYFEEIEYQTKQINRLKIWLKNLLIISSLIIAIIFFV
                     DKISMIITVISYIALIIIIIALIIINLAIRNGSMNVNNIIIKMEHIK"
     gene            complement(1100..1936)
                     /locus_tag="PLK68_05785"
     CDS             complement(1100..1936)
                     /locus_tag="PLK68_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM89957.1"
                     /translation="MEKKLSKKTLTKSFHNWYYGNLTCFSQEHMQTFGYLCSMLPIVE
                     ELYDKKDARAKAIKTYTAFFNTEPQVGSVIVGMTAGLEEARANGAEDVDDETINGLRA
                     GLMGPLAGIGDSLVVGTIIPILLGVAMGMSTGGSPLGAIFYIIVWNLFAYFGMKLLYF
                     KGYELGGKAVDFLVGPQGEALRESITMLGGIVIGAVAATWVSVKTSFSMTAAGAKEPF
                     LDLQKTLDGVYPGFLTALFVLLCWYLLSKKKMSPIKVMLLLVVIALIGVLVGFFNPGL
                     TY"
     gene            complement(1938..2774)
                     /locus_tag="PLK68_05790"
     CDS             complement(1938..2774)
                     /locus_tag="PLK68_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM89958.1"
                     /translation="MEISWIQAALLGLFACLSSMPGLGGTSFGNYTLGRPLVGGLVCG
                     IILGDIQTGILVGIAMQVVYIALVTPGGTVSADVRAVSYIGIPLAMIAIKSYGLDAAS
                     SDGAALATSFGTMVGTLGTVLFYGTATINLAWQHIGWRAVENGDYKKLYIVDMVLPWI
                     SHILCSFIPAMIMCKMGAPMVDLIKTYLPMDGVAMKTLFTVGSLLPCVGIAILLKQIV
                     TKAIDFIPFFFGFTLAAALGINLVSATVVAGMFALINYRIKMLTLGKVAVAVVDDDEE
                     DI"
     gene            complement(2802..3293)
                     /locus_tag="PLK68_05795"
     CDS             complement(2802..3293)
                     /locus_tag="PLK68_05795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM89959.1"
                     /translation="MSVSFVRIDDRMIHGQTCTRWAREYPCDGLIAVNDKAATNDVLK
                     AAYKAASGKKTFVWTLADFAKKSQKVLDSDTKYFLIAKNPIDMCTILVDQGFDPGVKT
                     VIVGPCNDREGATKLGNNQSITQAEADAFERIHKAGYTIKFALLPDVSIGTWDDFKSK
                     FGY"
     gene            complement(3306..3731)
                     /locus_tag="PLK68_05800"
     CDS             complement(3306..3731)
                     /locus_tag="PLK68_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM89960.1"
                     /translation="MKYVILVSHGNFAEGLLDSLKMLTGNHDDVIAIGLKDGITADQF
                     AIDFTEIITKIPKESGIIVLADIIGGSPLSTAMNVLAQSGLIDKTTVLGGMNLPLALT
                     TILMKDVLDTDALITNVTSEATGAIKQFVLETNDEDDDI"
     gene            complement(3873..>4444)
                     /locus_tag="PLK68_05805"
     CDS             complement(3873..>4444)
                     /locus_tag="PLK68_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM89961.1"
                     /translation="NALNRGFNITSNDFDQGKVAFAPMQFSQYRTYKPYPYRVSKYST
                     FEWDVIEMPQSSEQHLTQLSSLMMGMSMRTSAPELAWQLLKEFTYSQASQQDIYEYSQ
                     GVSPLKEVTESKQVLKLLDEDTLGDSKIDMRVLSQVMEYPTTNTRFRKYEAAMKIADN
                     QINQALQNNLDLDTSLTALQKEITNYLNE"
BASE COUNT         1500 a          905 c          551 g         1488 t
ORIGIN      
        1 caaacttttt aagctttcta aataataact tgttaaatct tgaaaactag tccaatattc
       61 tttctttaat tgatcaatca tttttctaaa ataattattt actaacggta aacttaatga
      121 aataatcagt agtattgtcg ctactagcag cgaagtatct tttaactgaa aaaataaata
      181 aactggcgca aagaaacaat acagcaaact tggtaaatac ttagtgtaat aaacctgcat
      241 tagctccact ccatcgccaa cactactaat tgtattactt gccccaatcc tttcaatttt
      301 accaacatct aattgaagca ttttttcaaa aatctcttta cgcaaattaa tccgtgcttt
      361 ggccccacag cgatattcgg cttctccagt taatagatca gctattaaaa tcaaacatga
      421 agcaatcaat gcactgatta ttgctgccag caactgctct tgtgacattt tcggatgata
      481 taaatcactt aaaaaggatg aaatagtttt agcaaacatt actgttccta ctaatgttaa
      541 tattttaagt ccggtaatta ttgcaatcca acctctaatt ccttcggaca ttctaattaa
      601 tgttttattt attaacagca tagtacacct ccaatgtact tatgttactc aagatttaat
      661 agtgtgtcaa actaaaaaca aatgtttcga aaaaaactag gaaattttaa aatttcctag
      721 tttccagcta atataagctc tattaaaatc atcagagccc ctttttttac taaaccataa
      781 atactccttt aggggcttgt ttaatttatt atttaatatg ttccatttta ataataatat
      841 tgtttacatt cattgaacca ttacgaatcg ccaagttaat aataatcaaa gcaattatga
      901 ttattattaa agcaatgtat gaaatcacag taataatcat acttatttta tctacaaaaa
      961 atatgatagc tataataaga gaggaaataa ttaataaatt ttttagccat atttttaatc
     1021 gattaatttg tttagtctga tattctattt cttcaaaata tgcttttttt actctttgat
     1081 ccattttaag ttctcctttt taatatgtta atcccgggtt aaagaatcca accaatactc
     1141 caataagagc aataaccact aataataaca ttactttgat tggtgacatt ttctttttag
     1201 acaataaata ccaacataat aacacgaata aagcagttaa aaatccagga taaacaccat
     1261 ctaacgtctt ttgtaaatct aaaaacggtt ctttagctcc agcagcagtc atcgaaaaac
     1321 ttgtttttac actaacccat gtagctgcta ctgccccaat cacaatacca cctaacattg
     1381 taattgattc tcgtaaagct tctccttgag gtccaactag aaaatctaca gctttaccac
     1441 ccaattcata acctttaaaa tataataact tcattccaaa ataagcaaat aaattccaaa
     1501 caataatata gaaaatagcc cctaatggtg aaccacctgt agacattccc attgcaaccc
     1561 ctaataaaat aggaattatc gttccaacta ctaaagaatc tcctattcct gctaacggtc
     1621 ccatcaatcc tgcacgtaat ccatttattg tttcatcatc tacatcttca gcaccattag
     1681 cacgagcctc ctctaacccc gctgtcattc caacgatcac ggaacctact tgcggttcgg
     1741 tgttaaaaaa ggcagtatac gttttaatgg cttttgcccg agcatctttt ttatcataca
     1801 attcttcaac aattggtaac attgaacaca aatagccaaa tgtttgcata tgttcttgag
     1861 aaaaacatgt taaattccca taataccaat tatgaaaaga tttggttagg gtcttttttg
     1921 ataatttctt ttccatctta tatatcctcc tcgtcatcat caactacagc aactgcgact
     1981 ttaccaagtg tcaacatttt aattcgataa ttgatcaatg cgaacattcc cgcaacaaca
     2041 gttgcagaaa ctaagttaat tcctaaagca gctgctaaag taaaaccaaa gaaaaatgga
     2101 ataaaatcaa ttgctttagt aacgatttgt tttaataaga ttgcaattcc gacacatggt
     2161 aataatgaac cgactgtaaa taatgttttc attgcaaccc catccattgg taaataggtt
     2221 tttatcaagt caaccatcgg agctcccatt ttacacatga tcatcgcagg aataaatgaa
     2281 cataaaatat gagaaatcca aggtaatacc atatctacta tatataattt tttataatct
     2341 ccattttcta ctgcccgcca gccaatatgc tgccatgcta gattaatcgt tgcagtacca
     2401 tagaataaaa ctgttcctaa agttccaacc attgttccaa atgatgttgc aagtgcagct
     2461 ccgtcacttg aggcagcatc tagaccatag cttttaattg caatcatagc aagtggaatc
     2521 cctatataac ttactgcccg tacatcagcc gaaactgttc cccctggagt aacaagagcg
     2581 atataaacta cttgcattgc aatccctact aaaattccag tttgaatatc acctaaaata
     2641 attccacaaa ctaaaccacc aactaaaggt cgacctaatg tgtagttacc aaatgatgtt
     2701 ccccctaaac ctggcatact tgataagcaa gcaaataatc caagtaaggc ggcttgaatc
     2761 caacttattt ccataataaa aatccccttt acatttatat tttaataacc aaatttactt
     2821 ttgaaatcat cccatgtccc aatcgaaacg tctggtaata aagcaaattt tattgtataa
     2881 ccagctttat ggatacgttc aaacgcatca gcctccgctt gagtaattga ctggttatta
     2941 cctaatttag tagccccttc acgatcatta caaggtccta caataacagt tttaacacca
     3001 ggatcaaatc cctgatctac taaaatcgta cacatatcaa ttggattttt agcaatcaag
     3061 aaatattttg tgtcactatc taaaactttt tgtgattttt ttgcgaaatc tgctaatgtc
     3121 caaacaaatg ttttcttacc acttgctgct ttataggcag cttttaaaac atcattagtt
     3181 gctgctttat catttacagc aattaatcca tcacaaggat attctctagc ccaacgcgta
     3241 caagtttgac catgaatcat ccgatcatca attcttacaa atgaaactga cataatcttc
     3301 tcctcctaaa tatcatcatc ttcatcattt gtttctaaaa caaattgttt aattgcacct
     3361 gttgcctcac tcgtcacatt tgtaattaaa gcatcagtat ctaaaacatc tttcattaat
     3421 attgttgtta aagctaatgg taaattcatt cccccaagta ctgtcgtttt atcaatcaat
     3481 cctgattgtg ccaatacatt cattgcagtt gataaaggtg aaccacctat gatatcagct
     3541 aaaacaataa ttccactttc tttaggaatt ttagtgatta tctcagtaaa gtctatagca
     3601 aactgatcag ctgtaattcc atcttttaat cctattgcaa ttacatcatc atgattacct
     3661 gtaagcattt ttaaagaatc tagtaaccct tcagcgaagt tcccatgact aactaatatt
     3721 acatatttca tttttagcct ccctttcttt tgacatctat atcttatcaa gtaacccctt
     3781 tcaatacatc tgagcattgt catttctttt cataaacaaa tgtcattttt ttaatgacat
     3841 ttgtcataat aaaaaacgaa tagtcatact tgctactcgt ttaaataatt agttatttct
     3901 ttttgtaaag cagttaatga agtatctaaa tccagattgt tttgaagtgc ttgattaatc
     3961 tgattatcag caatcttcat tgctgcctca tattttctaa atcgcgtatt tgtggttgga
     4021 tactccatta cctggcttaa tacccgcata tcaattttac tatcaccaag cgtatcttca
     4081 tctaacaatt tcaaaacctg tttagattcg gtcacctctt ttaatggaga taccccttga
     4141 ctatattcat aaatatcctg ctgtgaagct tgactataag taaattcttt taatagttgc
     4201 caagctaact caggagcact agttcgcatc gacatcccca tcattaaaga tgataattgt
     4261 gttaaatgtt gctcagaact ttgaggcatt tcaatcacat cccattcaaa tgtagaatat
     4321 ttacttactc gataaggata aggcttatat gttcgatatt gagaaaactg cattggtgca
     4381 aaagctactt tgccttgatc gaaatcattg gaagtaatat taaagccgcg atttaaagca
     4441 ttca
//
LOCUS       DAOMTA010000166         4438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_561_length_4438_cov_2.766598, whole genome shotgun sequence.
ACCESSION   DAOMTA010000166 DAOMTA010000000
VERSION     DAOMTA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_561_length_4438_cov_2.766598"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1571
                     /locus_tag="PLK68_05810"
     CDS             243..1571
                     /locus_tag="PLK68_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM89962.1"
                     /translation="MLFDNKALKKLIIPLVVEQLLVITVGFADTVMVASVGEAAVSAV
                     SLVDAINILLINIFAALGTGGAVVAAQFIGQGSKDKAKYSAKQLILITALLSVIIMVI
                     VIAFNVPLLHMVFGNVEVDVMKNAEIYFLFSAMSYPFIALYNSGAALFRAIGNSKISM
                     INSAVMNVINIILNAIFIFVFRWGVFGAVLATLIARAVACIVILKMLSHRDNDVCVND
                     YLHWKFDFMYIKKILAIGIPSGLENGMFQLGKILVQSLIATFGTYSIAANAVSNNLAQ
                     MQIIPGMAMSLAMVTVVGQCVGANDYKQAKYYVKKLLKITYLSMAGLIVVLIIATPSI
                     LTFYSLSKETTDLAYQCIFLHAIIAALIWPTSFTFPNALRAANDAKFTMIVSAASMLI
                     FRLCFSYVLGQYMNLGLIGVWIAMFIDWGVRCIFFIWRYFSGKWMNRQLV"
     gene            complement(1760..2248)
                     /locus_tag="PLK68_05815"
     CDS             complement(1760..2248)
                     /locus_tag="PLK68_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HRM89963.1"
                     /translation="MTKRITYLSLLIAIAIILGYLETFIPINFGIPGAKLGLANLIGL
                     IALYRFGFRDALIITLLRVFIIASVFTNYYMFLYSLSGALLSLVIMVLLKKTKLFSTL
                     IISISGAIFHNLGQILVALIFYGFNIIYYLPYLIILSLITGSVIGILGQIILAKLPKQ
                     LS"
     gene            complement(2258..2617)
                     /locus_tag="PLK68_05820"
     CDS             complement(2258..2617)
                     /locus_tag="PLK68_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRM89964.1"
                     /translation="MFKKKDFIIIFIIFVVIIGALLFNHFYFSQKAAYVKITVNNQVY
                     RTVPLEIDQTIKISDKNMIVINHGSVHMKDATCPDKLCIKQGTISKNGEQIICLPNQV
                     VIAIVSNQYNDVDSSSK"
     gene            2646..3620
                     /locus_tag="PLK68_05825"
     CDS             2646..3620
                     /locus_tag="PLK68_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM89965.1"
                     /translation="MKKRWFIKILIILIIFCGCANKQPLSQTQLLLDTQVTITLYDRA
                     SSNILDECFAICKDYELLFSRTNPKSELYQLNHQDKTKPIKISKELAKVINIGLEYSK
                     LSNGTFDITVGQLIDLWDFKADTPKLPETSAIAGALTSIGYRGITLNDSTISFSNPNT
                     IIDLGAVAKGYIADKIKEYLIEQGVDSAIINLGGNVLCVGKKNSDDFTIGITDPKGSS
                     DILKLKINDQSVVTSGIYQRYFEVEGKYYHHILNPKTGYSYDNGLASVTIISNHSVDG
                     DALSTVCFTLGKEQGLALVNQLAGIEAVFIDTNNALYYSDHAKDYVIQ"
     gene            3692..>4438
                     /locus_tag="PLK68_05830"
     CDS             3692..>4438
                     /locus_tag="PLK68_05830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM89966.1"
                     /translation="MNTKIAFFDIDGTLVNVPNGMLHPTDETIRVLNEFKNQGNKIVI
                     ATARGEVPESVANIEFDGYICNDGHYIRFNNEILIDEQFDNGMVQKQLDVYAKYNGRS
                     MFNGRGGAWCSFLDDELVIKHRAMFQGTTERPTDVNEVFKTSDVKAVSCCVLFDSAAQ
                     LWAAYHELEDEFTMIPYDTGLIRMDVYCKGFTKGTACEYLYQKLGIDYENTYAFGDGI
                     NDVEMLQLVKHGIAMGNAIPKLKSVASEITD"
BASE COUNT         1505 a          539 c          874 g         1520 t
ORIGIN      
        1 aaaatgaagt gtgtacactt aaacgaattg cttttgcagg aatagaactt gatcaaaaat
       61 tagctttagg ggaatatcga gacttgacct caacagaatt gataactttg tttaagcatt
      121 gttagactaa taatttgttt ttagacctca tttgtgaggt ttttttgctt ttccagaaaa
      181 tgaaatttac tttgttcaaa agaatatggg cgtataatag gtaactgtag ggggcgtgag
      241 gaatgttatt tgataacaaa gcattaaaaa aattaattat tcccttggtt gtggaacaat
      301 tattagtaat tacagtaggt tttgcagata ctgttatggt tgctagcgtc ggcgaagcag
      361 cagtatcagc agtttcgctg gttgatgcaa ttaatatttt attaatcaat atttttgctg
      421 cattaggaac agggggagca gtagttgcag cccagtttat tggacagggt tctaaagata
      481 aggcaaagta ttcagcaaaa cagttgattt tgattacagc attattgtca gtaattatca
      541 tggtgatagt tattgcattt aatgtgccat tattacacat ggtttttgga aatgttgaag
      601 tagatgtaat gaaaaatgca gaaatctatt ttttattttc agcaatgtcg tatcctttta
      661 ttgcattata taactctgga gcagcattat ttagggcaat tggtaactct aaaatatcga
      721 tgattaatag tgcagtcatg aatgttatta atattatttt aaatgcaatt tttattttcg
      781 tttttagatg gggggtattt ggtgccgtat tagcaacgct gattgccaga gcagtagctt
      841 gtatcgtaat tttaaagatg ttaagtcatc gggataatga tgtatgtgta aatgattatc
      901 ttcattggaa atttgacttt atgtatataa aaaagatttt agcaattggg ataccttcgg
      961 gattagaaaa tgggatgttt cagctaggga aaatcttagt acaaagttta attgcaactt
     1021 ttgggacata ttcaattgca gctaatgctg tttcgaataa cttggctcaa atgcagatta
     1081 ttcctgggat ggcaatgtct ttggcgatgg tgacggttgt aggtcaatgt gtaggtgcaa
     1141 atgattataa acaagcaaaa tattatgtta aaaagttatt aaaaattact tatctttcaa
     1201 tggctggatt aattgtagtt ttaattattg caacaccttc aattttaaca ttctattcat
     1261 tatcaaaaga aacgacggac cttgcatacc aatgtatctt tttacatgca attattgcag
     1321 cattaatctg gcctacgtca ttcacatttc caaatgcttt aagagcagct aatgatgcta
     1381 agtttacgat gatagtttca gctgcatcaa tgctaatatt tagattatgc tttagttatg
     1441 ttttgggaca atatatgaat cttggattaa ttggtgtttg gattgcaatg tttattgatt
     1501 ggggagttcg ttgtattttc tttatctggc gctattttag tggaaaatgg atgaatcggc
     1561 aattagttta ataaaaaaag attgacttaa agtaatgttt aaggagtata gtactatcgt
     1621 agcaaagtaa gtgatcatgt agattatttt tctctcccaa tataaaatat tacatgatca
     1681 tttcatttgc tgctaaaaat gagagttata ttgttgtttg aaaaggaaga aattcctttt
     1741 tttagtaaaa aacaattcat tatgatagtt gtttaggtag tttggctaaa ataatttggc
     1801 ctaagatacc aatgacacta cctgtaataa gtgataagat tattaaataa ggtagataat
     1861 aaataatatt aaaaccatag aaaattaagg ctactaggat ttgtcctaga ttatgaaaga
     1921 ttgcgccgga aatactaatg atcaaagttg aaaagagttt ggtttttttt aaaagaacca
     1981 taatgactaa acttaataaa gcaccgctta aactatataa aaacatatag tagttagtaa
     2041 aaacactggc aataataaaa actcgcagga gagtgatgat taaagcgtct ctaaatccaa
     2101 agcgatatag tgcaattaat ccgatgaggt tagcaaggcc gagttttgca ccaggaattc
     2161 caaagttaat aggaataaag gtttcaagat aaccgagaat aattgcgatt gcgattagta
     2221 aagataaata ggtaattctt ttagtcattg gggcctccta tttggatgat gaatcaacat
     2281 cgttatattg attagaaaca attgcaataa caacttgatt aggcaaacaa ataatttgtt
     2341 caccattttt agatatcgtt ccttgtttga tacataattt atcagggcaa gtagcatctt
     2401 tcatatgtac acttccgtga ttgatcacga tcatattttt atcactaatc ttaattgtct
     2461 gatcaatctc tagaggaaca gtacgatata cttgattatt aacggtgatt ttcacgtaag
     2521 cagctttttg actgaaataa aaatgattaa aaagcagtgc cccgataata acgacaaaaa
     2581 ttatgaaaat aataatgaag tctttcttct taaacatagc tacctccaga atgaaaggaa
     2641 atgacatgaa aaaacgttgg tttataaaaa ttttaataat cttaattata ttttgtggat
     2701 gtgcgaataa acagcctcta agtcaaacgc agcttctttt agatacgcaa gttacgatta
     2761 ctttatatga tcgtgcttcg tctaatattc tagatgaatg ttttgctatt tgcaaggact
     2821 acgagcttct cttttcccgg actaatccca aaagtgaatt atatcagctc aatcatcaag
     2881 ataaaactaa accaattaaa atatccaagg aacttgctaa agttataaat attggtttgg
     2941 aatatagtaa attatctaat ggtacttttg atattacagt aggacaattg attgatttgt
     3001 gggattttaa agctgatact ccaaaattgc cggaaacttc agcaatagcc ggtgctttga
     3061 cgtcgattgg atatagggga attactttaa atgatagtac aattagtttt agtaatccta
     3121 atactataat agatctaggg gctgttgcaa agggatatat tgctgataaa attaaggaat
     3181 acttaattga gcagggagta gacagtgcaa ttatcaattt aggtggtaat gttctttgtg
     3241 tagggaaaaa gaatagtgat gattttacaa ttggtattac tgatcctaaa ggaagtagtg
     3301 acattttaaa attaaaaatt aatgatcagt cggttgtaac ttcaggaatt tatcaacgtt
     3361 attttgaagt tgaagggaag tattatcatc atatcttaaa tccgaagacg ggatatagtt
     3421 atgataatgg gttagcttca gttacgatta ttagtaatca ttcagttgat ggtgatgcat
     3481 taagcacagt atgttttact ttaggtaaag agcaggggtt agcattagta aatcaattag
     3541 cggggataga agccgtgttt attgatacaa acaatgctct ttattacagt gatcatgcaa
     3601 aagattatgt gattcagtaa ttattttcat aatattaaaa tatctcataa aagatatgaa
     3661 tttctttata cttaaaagaa ggaggagcat tatgaatacg aaaatagcgt tttttgatat
     3721 tgatgggaca ttagttaatg tacctaatgg gatgttacat ccaactgatg agacaataag
     3781 ggttttaaat gaatttaaaa atcaaggtaa taagatcgtc attgcaacag ctcggggtga
     3841 agtacctgaa agtgtggcaa acattgaatt tgatgggtat atttgtaatg atggacatta
     3901 tattcgtttt aataatgaga ttttgattga tgaacaattt gataatggaa tggtgcagaa
     3961 gcaattagat gtatatgcaa aatataatgg tcgttcaatg tttaatggtc gcggtggtgc
     4021 atggtgcagt tttcttgatg atgaattggt tataaaacat cgtgctatgt ttcagggaac
     4081 aactgaaaga ccaactgatg ttaatgaggt atttaaaact agtgatgtta aagcggtaag
     4141 ctgttgtgtt ttatttgata gtgcagcaca gttgtgggca gcttatcatg aattagaaga
     4201 tgaatttacg atgattccgt atgatacagg tttgattcga atggatgttt attgcaaagg
     4261 tttcactaag ggaactgctt gtgaatattt atatcaaaaa ttaggaattg attatgaaaa
     4321 cacgtatgct tttggtgacg gtatcaacga tgtagagatg ttacaattgg ttaaacatgg
     4381 tattgctatg gggaatgcta ttcctaaatt aaaaagtgta gcaagtgaaa taactgat
//
LOCUS       DAOMTA010000167         4416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_569_length_4416_cov_2.235955, whole genome shotgun sequence.
ACCESSION   DAOMTA010000167 DAOMTA010000000
VERSION     DAOMTA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_569_length_4416_cov_2.235955"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1660)
                     /locus_tag="PLK68_05835"
     CDS             complement(14..1660)
                     /locus_tag="PLK68_05835"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HRM89967.1"
                     /translation="MNKLVSNIEKLKPFFEKVSRNRYLRAIRDGFISAMPIVLFSSIF
                     MLLAYVPNIFGFYWSKEIEAMILKPYNYSMGILALVVAGTTAKNLADNFNRDMPVNRQ
                     INNISALMAAIVGFLIVGVDSIEGGFANGYMGSKGLLTAFLVAFVVCNVYRFCVKRNV
                     TIKMPDAVPPNISQTFADVIPFAVSAIIFTIFDIIFRNVTGICFAQGVIEFFQPLFTA
                     ADGYIGLAIIYGAMSLFWFVGIQGPSIVEPAVSAIYYVNIANNLDLFQAGQHANNILT
                     PGVQQFVATIGGTGATLVITLMFAFMSKSKELKAIGRASSIPVIFGVNEPILFGAPLI
                     LNPIFFIPFIFAPILNVWLFKAFVDFLGMNSFMYVLPWTTPGPIGIVLGCGLGLLTIL
                     FAAVILLVDFAIYYPFFKVYDNEKCEEEKNKNLDAVEKEEEMIEVDGNTLKSKRILVL
                     CAGGGTSGLLANALAKGAKEQNIPLITAAGSYGAHMDIMKDYDLVVLAPQVANYYEDL
                     KKDTDRLGIKCVACEGKQYINLTRDPDGALKFVFKIMEGE"
     gene            complement(1682..1990)
                     /locus_tag="PLK68_05840"
     CDS             complement(1682..1990)
                     /locus_tag="PLK68_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM89968.1"
                     /translation="MTKEEATMVAFEIVAYSGDARSKLLMAVEKAKAGDLVTADRLVA
                     EANECLVDAHKAQTDLLQQEARGDNVEIGFIMVHGQDHLMTTLLLKDIVGTLMDVYKK
                     "
     gene            complement(2010..>4416)
                     /locus_tag="PLK68_05845"
     CDS             complement(2010..>4416)
                     /locus_tag="PLK68_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM89969.1"
                     /translation="SLLHSNFGYNQSLNEAVMLAKAAILYPQGSPHILLTAESGSGVK
                     YFAKNVYDFAVKSKVLKNNAPFTVFDCKTFIENPTIINEMLYGDEVNTGLVHQTNQGM
                     LLIKHVELLSGYQRTMLFSIITSNKMPSNQYVELPRNYKCIMLCAIASDASKDLYDLY
                     RNKMDFVIELLPLSQRPLKERFALLELFLKQEARKLDRVLEVSTNILHSLLLYEVKDN
                     IRGLKNDIHTGVANCYVREHDVHHHHIELLLSDFPNYVRKGMIYYKTFKEEIDEIIPG
                     DCKFAFTKNEVLKNRAKENNANIYRTIDVRKKELKKQSVSEEQINTLVSLQLHQDFQE
                     YLNELSNRVSDKEQLSKIVSMKLISLVERFITRVSSELAINYQENILYGLCLHINASL
                     IKVSSKQRLANEEIKRMIDLYPKHYHLAKEFVHEIEEEFRVKMNIDEIIFTMLFILNE
                     SKPVTNKHVVTLIAMHGDSSASSIVNVVNALAMHNNTYAYDLPLDKSMDDAYEDLKEQ
                     IIKVDQGKGIILIYDMGSIRTMAESIAFETKIEIKYLEMPVTLIGVTSSNKASNNDSV
                     DEIYEYLQTKFKDIKYFRKQSDKKILVIISKEQSEVTRLKSYLNEHFDLSNVTVHVIE
                     NSEANHLYNEINIIANEGKIIGIIGNNRPNLAQYPFAEVRWLEQKNAKTLEEIFIEED
                     EARDDINEIFEYLKDQFNEVDVDGIKDYLLDFTKSLEVTLDATLDEDQQIGLIVHLVC
                     LIDRISRHQAPIVNFIASNIILNHGMLVSKVKELLVPLEMALNISISDAEIATIISIV
                     KKR"
BASE COUNT         1501 a          834 c          544 g         1537 t
ORIGIN      
        1 ggaaatttca tttttattct ccctccataa ttttaaacac aaatttcaag gccccatcag
       61 gatctcttgt tagattgata tattgtttac cttcacaagc aacacattta attcctaaac
      121 gatctgtatc ttttttcaaa tcttcataat aattagctac ttgtggtgcc aatacaacta
      181 aatcataatc tttcataata tccatatgtg ctccataaga accggcagct gtaatcaatg
      241 gaatattttg ttctttagcg cctttagcta aagcatttgc taataaacca cttgttccac
      301 caccagcaca taaaaccaag attcgttttg attttaaagt atttccatct acttcaatca
      361 tttcttcttc tttttcaact gcatctaagt tcttattctt ttcttcttca cacttttcat
      421 tatcataaac tttaaaaaat ggataataaa tagcaaaatc aactagtaga ataactgcag
      481 caaaaagaat tgttaataaa cctaaaccgc atcctaaaac aatcccaatt ggtcctggtg
      541 ttgtccaagg taatacatac ataaaactat tcatgcctaa gaaatctaca aaagctttaa
      601 ataaccaaac atttaaaatc ggagcgaaaa taaatggaat aaagaaaatt ggatttaaga
      661 taagcggtgc cccaaataaa attggttcat taaccccaaa gattaccgga atcgaactag
      721 cacggccaat tgcttttaat tcttttgatt ttgacataaa tgcaaacatc aatgtaatta
      781 caagagttgc accagtccca ccaattgttg caacaaattg ttgaactcct ggtgttaata
      841 tattattagc atgttgtccc gcttggaata aatctaaatt gtttgctata tttacataat
      901 agattgccga aactgccggt tccacgattg atggaccttg aattcccaca aaccagaata
      961 atgacattgc cccataaata atagccaacc cgatataccc atctgctgca gtaaatagtg
     1021 gttgaaaaaa ttcaattaca ccttgagcaa aacatattcc agttacattc ctaaaaataa
     1081 tatcaaaaat agtaaagatg attgctgaaa ccgcaaacgg tataacatca gcaaaggttt
     1141 gagaaatatt tggtggtact gcatctggca ttttaatagt tacatttcgt tttacacaaa
     1201 aacgataaac attacaaacg acaaaggcaa ctaaaaaggc tgttaataac ccttttgacc
     1261 ccatatagcc atttgcaaac ccgccttcga tcgaatcgac cccaacaatc aagaaaccaa
     1321 caattgctgc cattaacgca gaaatattat tgatttgacg attaaccggc atatcacgat
     1381 taaaattatc tgctaaattc ttagccgtag tacctgcaac aacaagagct aaaatcccca
     1441 ttgaataatt atatggtttt aagatcattg cttctatttc tttagaccag taaaaaccaa
     1501 agatatttgg tacatatgca agtaacataa aaatacttga aaataataca atcggcatgg
     1561 ccgaaatgaa accatcacga atcgctcgta aatagcggtt acgtgaaact ttttcaaaga
     1621 aaggttttaa tttctctata tttgaaacta atttattcat atacgatccc ccgttaaatt
     1681 tttatttttt atatacatcc attaaagtcc caacaatatc ctttaataat aatgttgtca
     1741 tcagatgatc ttgaccatga accatgataa aaccaatctc aacattatcc cctcgagctt
     1801 cttgttgcaa taaatctgtt tgagctttat gagcatctac taaacattca ttagcttctg
     1861 ctactaatct atcagcagtc actaagtcac ctgctttagc tttctctaca gccattaata
     1921 attttgaacg agcatcccct gagtaagcta cgatttcaaa ggcaaccatt gttgcttctt
     1981 ctttagtcat atttaatcct tctttctatt tatctctttt taacgattga aataatcgtt
     2041 gcaatttcag cgtcactgat tgatatattt aaagccatct ctaaaggaac taaaagttcc
     2101 ttgaccttac taactaacat tccatgattt aaaattatgt ttgatgcaat aaaattaaca
     2161 atcggtgctt gatgtctact gattcgatca atcaaacaaa ccaagtgtac tattaaaccg
     2221 atttgctgat cctcatctaa agttgcatct agtgtaactt ctaaactctt agtaaaatcc
     2281 aatagataat cttttattcc atcaacatct acctcattga actgatcctt taaatattca
     2341 aatatttcat taatatcatc acgtgcttca tcttcctcaa tgaatatctc ttctaaagtt
     2401 ttagcattct tttgttctag ccatctcact tcagcaaaag gatattgagc taaatttggt
     2461 ctattattac caataattcc gataattttt ccttcattag caataatatt gatttcattg
     2521 tatagatgat tagcttcact attttcaatt acatgaacag taacattact taaatcaaaa
     2581 tgttcattta aataactctt taaccttgta acttctgact gctcttttga aattattact
     2641 aagatcttct tatcactttg ttttctaaaa tattttatat ctttaaactt tgtttgaaga
     2701 tattcataaa tttcatcaac tgaatcatta ttactggctt tattactgct agtgacacca
     2761 attaaagtaa ctggcatttc taaatattta atttctattt ttgtttcaaa agcaattgat
     2821 tcagccatag ttcgaattga acccatgtca taaataagaa taatcccttt accctgatca
     2881 acctttataa tttgctcttt caaatcttca taggcatcat ccatactttt atctaaaggc
     2941 aaatcatagg cataagtatt gttatgcatt gctaaagcgt taacaacgtt aacaatcgaa
     3001 ctagccgaac tgtcaccatg catcgcaatc aaagtaacaa catgtttatt agttacaggc
     3061 ttactctcat ttaaaataaa cagcattgta aagattattt catcaatatt catctttacc
     3121 ctaaattctt cttcaatttc atgaacgaat tctttagcaa gatgataatg tttaggataa
     3181 agatcaatca tccgcttaat ttcttcattt gcaagacgct gtttactgct tactttaatt
     3241 agtgaagcat taatatgcag acataaccca taaagaatat tttcttgata atttattgct
     3301 agttcactgc ttacacgagt aatgaaacgt tcaactaaac taattaattt cattgaaaca
     3361 attttactta attgctcttt atcacttaca cgattactca attcatttaa atattcttga
     3421 aaatcttgat gaagctgtaa tgaaactaaa gtattaattt gttcctcact aacactttgt
     3481 ttttttaact cttttttacg aacatcaatc gttcgataaa tattagcatt attttccttt
     3541 gctctatttt ttaatacctc atttttagta aatgcaaatt tacaatctcc aggaataatc
     3601 tcatcaattt cttctttaaa tgttttatag taaatcattc ctttgcgaac ataattagga
     3661 aaatctgata acaataactc aatatggtga tgatgaacat catgttctct tacataacaa
     3721 tttgctactc ctgtatgaat atcatttttt aaaccacgga tattgtcctt cacttcatat
     3781 aataataagg agtgtaatat attagtgctt acttctagaa cccgatcaag ttttctggct
     3841 tcttgtttta aaaacaattc taaaagagca aatcgttctt ttaagggtct ttgacttaat
     3901 ggcaaaagtt caataacaaa atccatttta ttacgataga gatcataaag atccttactg
     3961 gcatcactgg ctattgcaca aagcatgata catttataat tacgcggtaa ttctacatat
     4021 tgattagagg gcatcttatt acttgtaatg attgaaaata acattgttct ttgataccct
     4081 gataaaagtt caacatgttt aatcaacagc atgccttgat tagtctgatg tactagtcca
     4141 gtattcactt catcaccata aagcatttca ttaataatcg taggattttc aatgaatgtt
     4201 ttacagtcga aaactgtaaa tggtgcatta ttttttaata ctttagattt aactgcaaag
     4261 tcataaacgt ttttagcaaa atatttaacc ccacttccac tttctgcagt taagagaata
     4321 tgaggacttc cttgaggata aagaatggca gcttttgcta gcatcactgc ttcattcaaa
     4381 ctttggttat aaccaaagtt tgaatgaagc agtgat
//
LOCUS       DAOMTA010000168         4410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_571_length_4410_cov_2.690241, whole genome shotgun sequence.
ACCESSION   DAOMTA010000168 DAOMTA010000000
VERSION     DAOMTA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_4410_cov_2.690241"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLK68_05850"
     CDS             complement(<1..570)
                     /locus_tag="PLK68_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HRM89970.1"
                     /translation="MKDKLKNFKHLFFKILKFEIFYKFAIFLIISPLINKILQIYLNN
                     NSSGAAFNQYILFNFLNLEGITVVLIIMMMAVIAIGYEFSVLINMITLNKQNKDFRIY
                     EVMKTSLLNLSCLKHPSSILTGIFFLFILPLVHLGYINSLIPSLKIPNFIFGELSLTF
                     QGNILICLIYGLYFGIYGLMFFVPVLMILK"
     gene            complement(670..1125)
                     /locus_tag="PLK68_05855"
     CDS             complement(670..1125)
                     /locus_tag="PLK68_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89971.1"
                     /translation="MIHLVYLDNKEKVLEKIMSGKKTMIIRGAAGRKIPHSRVFNDEV
                     LYFMEKGTKKITAKARVVNVLNFIKLSEDEINEILLKYQDKLDLTPKQQVRWHKKCLC
                     LVEFTDVEAIKPLDFDHQGNMDDWLILDRIEDVVVGTSIPYNYNNARFK"
     gene            complement(1170..2015)
                     /locus_tag="PLK68_05860"
     CDS             complement(1170..2015)
                     /locus_tag="PLK68_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM89972.1"
                     /translation="MNTLMMEIIMDKEYYSIGEIAEICNIPIRTLRYYDEIGLLVPEK
                     RDIESSYRYYARHQILQANIINQFKIQGYSIKEIKHQLANNSVSLSEETLNQKHAELK
                     KTIAELNKLEKRLGFFLECLKYQDHQLHLQLKEIPEIYIAYVRQQGLADQAAFMRRFS
                     ELNTLCRKNNLEPIGNIMARYYDDYQQYDPDCADIEVSIQIDLNHEIGGVVRKEPGYL
                     CVSALHYGPYRDEYKTYTLMMEWMKKNNLVMCGPALEYYLIDPIFTNDENEFVTELRI
                     PVKSI"
     assembly_gap    2068..2077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2078..2318)
                     /locus_tag="PLK68_05865"
     CDS             complement(<2078..2318)
                     /locus_tag="PLK68_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89973.1"
                     /translation="MNDEVNVRPFAYAMSLIDGKWKMHILFWLWKKQVLRYSELKRAL
                     GTITHKMLSTQLKELEKDDLIIRNEYPQVPPKVEYS"
     gene            2423..2560
                     /locus_tag="PLK68_05870"
     CDS             2423..2560
                     /locus_tag="PLK68_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89974.1"
                     /translation="MKKQINVFDYAKEIMEAVQTGVLLTTKVDDKVNSMTISWGTLGI
                     E"
     gene            2582..2959
                     /locus_tag="PLK68_05875"
                     /pseudo
     CDS             2582..2959
                     /locus_tag="PLK68_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
     gene            complement(2996..>4410)
                     /locus_tag="PLK68_05880"
     CDS             complement(2996..>4410)
                     /locus_tag="PLK68_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM89975.1"
                     /translation="YFKEMGFNCLRVSIAWSRIYPNGDELEPNEKGLQFYDQLFDEML
                     KLDIEPVVTLSHYEMPYYLSRQYNGFFDRRCVDYFERFAITCFKRYSAKVKYWLTFNE
                     INGMITNPYTGGGVIAKIDNNYLQTVLTACHHMFLAGAKAVKACHEIIPSAQIGCMIA
                     FLCGYSATCKPDDVMFNHNFMDVNMFFTDVQVRGRYSNKALTWMSRYGIELPVIDGDE
                     KILEQGKVDYIGFSYYQSITMSSDILDNLGSGGNLFSGAKNSYIKVSEWGWPIDPKGL
                     RVSLNYLYDRYQIPLFIVENGLGAVDEISDDHQIHDNYRIDYLTQHVREMKKAVDLDG
                     VELLGYTWWSPIDIVSYSTGEMKKRYGFIYVDKDNDGNGTLKRYIKDSFYVYQEIIRT
                     NGECVDEKKRYTLNSQLKDVLEIKGLREIIKLVSEGKVTDLQLKLGGRLKINQLLDKF
                     DVNDNNQRLIIDLLNRLEAK"
BASE COUNT         1510 a          792 c          530 g         1568 t
ORIGIN      
        1 ttttaaaatc attaaaaccg gtacaaaaaa cattaatcca taaattccaa aatacaatcc
       61 atagattaaa catattaaaa tatttccttg aaaagttaag ctcaattctc caaaaataaa
      121 attaggaatt tttaaacttg gtatcaatga gtttatataa ccaagatgca ctaatggtaa
      181 aataaataaa aagaatattc ctgttaaaat actactagga tgctttaaac aactcaaatt
      241 taataacgag gttttcatta cctcataaat tcgaaaatct ttattttgct tatttaaagt
      301 aatcatattg atcagtacac taaattcata tccaattgca attactgcca tcatcataat
      361 aattaaaaca actgtaatcc cttcaagatt taagaaatta aataatatgt attggttaaa
      421 tgcagctccg ctggaattat tatttaaata tatttgaaga attttattaa tcaatggact
      481 gataattagg aatattgcaa atttatagaa aatctcaaat tttaggattt taaaaaagag
      541 atgtttaaag ttttttaatt tatctttcat tttcacaccc ccaatatgac ttattctatc
      601 attattacca atttattgtt attaataatc cttaaaaatt ttcttaaaaa aaactaccct
      661 tccggcagtt tacttaaatc tagcattatt atagttgtag gggatactcg ttcctactac
      721 aacatcttca attctatcaa gaatcaacca atcatccata ttcccctgat gatcaaaatc
      781 tagtggttta attgcttcta catctgtaaa ttcaactaaa cacaaacatt ttttatgcca
      841 gcgaacttgt tgttttggcg ttaaatctaa tttatcttga tactttaata atatttcatt
      901 tatttcatct tcacttaatt taataaaatt taaaacgtta actactcttg cttttgctgt
      961 tattttttta gttccttttt ccatgaaata taatacttcg tcattgaata ctctactatg
     1021 aggaatcttg cgtcctgctg ctcctcgaat tatcattgtt tttttgccac tcataatctt
     1081 ttctaagact ttttctttat tgtctaaata tactaaatgt atcatctaat cacctcaaat
     1141 ttattataaa gcctctagta actagagagt caaatgcttt ttacaggaat tcttagttca
     1201 gtcacaaatt cattttcatc attagtaaag ataggatcta ttaaataata ttctaatgct
     1261 ggcccacaca ttactaaatt attttttttc atccattcca tcataagagt ataagtctta
     1321 tattcatctc gataaggccc ataatgaaga gcagaaacac ataaataacc aggttctttt
     1381 ctaactactc caccaatttc atgatttaaa tcaatttgaa tagaaacttc aatatcagca
     1441 caatcgggat catattgctg ataatcatca taataacgcg ccattatatt tcctatcggc
     1501 tctaaattat tcttacgaca taaagtattt aattcactaa atcgacgcat aaaggctgct
     1561 tggtctgcta aaccttgttg acgaacataa gcaatataaa tttccggtat ttcctttaat
     1621 tgtaaatgca actgatgatc ttgatacttt agacactcta aaaagaatcc taaacgtttc
     1681 tctaatttat ttaattcagc tattgttttt tttaattcag catgtttttg attcaaggtt
     1741 tcttcactca aagaaacgga attatttgca agctgatgtt taatctcttt aattgaatac
     1801 ccctgaatct taaattgatt aataatatta gcttgtaata tctgatgtct agcgtaataa
     1861 cgatagcttg attcaatatc ccttttttca gggactaata gcccgatttc atcatagtaa
     1921 cgtaaggtcc ttatcggaat attacaaatt tctgcaatct cacctatcga ataatattct
     1981 ttatccatga ttatctccat cattaatgtg ttcatgtccc cacttgcata aacattctaa
     2041 caccggcatc aatgttaaac cacgtggnnn nnnnnnnatg aatattcaac tttaggcggg
     2101 acttgcggat actcattgcg aatgatcaag tcgtcctttt ccaattcttt taactgagtg
     2161 cttagcattt tatgtgtaat tgttcctaat gcacgtttta actcactata tcgtagtact
     2221 tgttttttcc ataaccaaaa taatatgtgc attttccatt tgccatcgat caaagacatt
     2281 gcatatgcaa atggtcttac atttacttca tcgttcattt ttactctcct tttagatagt
     2341 atcttactta aaagtgtata cttgttatta atttagttct aatttatcat atagttatta
     2401 aaataacaag gaggctaatt agatgaaaaa acaaataaat gtatttgatt atgctaaaga
     2461 aattatggaa gctgttcaaa ctggcgttct tttaacaact aaagttgatg ataaagtcaa
     2521 tagtatgaca atttcctggg gaaccttagg aattgaatga gcaacagaat tatttactgt
     2581 ttttgtaaga gaacatcgtt tcactaaaga acagatagaa aaaaatcctg aatttacaat
     2641 caatattcct ttaggtgctt ttgataagaa aatcttaggg gtttgcggta cgaaatcagg
     2701 aagagatatt gataaagtta aagaacttgg gcctacttta gaagatcctg ataaaatatc
     2761 agttccaggt attaaagagc ctcctttgac acttgaatgt aaaattgtct atcaacaagt
     2821 acaagataaa aatgcaatta ctccaaacaa tttagaacgt ttttatcctc aagatgttga
     2881 tagttcattc tatggagcaa ataaagattt acacacggct tattatggcc aaatcattaa
     2941 tgcttatatt attgaataaa tagaaaagtc cggttaatta ttccggacat tttcattatt
     3001 tagcctctaa acgattcagt aaatctataa tcaatctttg attattatca ttaacatcaa
     3061 atttatcaag taattgattt atttttaagc gtccccctag ttttaattga aggtcagtaa
     3121 cttttccttc actaactaat ttaataattt ctctaagccc ttttatttcc aaaacatcct
     3181 ttaattgact atttaaagta taacgttttt tttcatcaac acactcacca tttgttctta
     3241 taatttcctg atatacataa aatgaatctt taatataacg ttttaaagta ccatttccat
     3301 cattatcttt atctacgtaa ataaatccat aacgtttttt catttcgcca gttgaatagc
     3361 ttacaatatc aatcggactc caccaagtgt acccaagcaa ctcaactcca tctaagtcta
     3421 ctgctttttt catttctcga acatgttgcg tcaaataatc aattcggtaa ttatcatgaa
     3481 tttgatgatc gtcactaatt tcatcgactg ctcctagtcc attttctacg ataaataacg
     3541 gaatttgata acgatcatat aagtaattca aagaaactct aagtccctta ggatcaatcg
     3601 gccagcccca ttcgcttact ttaatatatg agtttttagc tccactaaaa agatttccgc
     3661 cacttccaag attatctaaa atatcacttg acatcgtaat actctggtaa taactaaaac
     3721 caatataatc aacttttcct tgttctaaga ttttttcatc accatctatg actggtaatt
     3781 caattccata acgtgacatc catgtcaatg ctttatttga atatctccct cgtacctgaa
     3841 catcggtaaa aaacatattt acatccataa aattatgatt aaacattaca tcatcgggtt
     3901 tacatgtagc gctataacca cataagaagg caatcataca gccaatttga gcagaaggaa
     3961 taatctcatg acaagcttta accgcttttg ctcctgctaa gaacatatga tgacatgcag
     4021 tcaaaacggt ttgtaaataa ttattatcaa tttttgcaat cacaccacct ccagtataag
     4081 gattggtgat cattccatta atctcgttaa aagttagcca atatttgact ttagcagaat
     4141 atcttttgaa acatgtaata gcaaaacgtt caaaataatc aacacaccgc cgatcaaaaa
     4201 agccattgta ctgcctgctt aaataataag gcatttcata atgcgataat gtgaccactg
     4261 gttcaatatc tagtttcaac atctcatcaa ataattgatc ataaaactgc aacccttttt
     4321 cattcggctc tagctcatca ccattaggat agatacgtga ccaagcaatt gacactcgca
     4381 aacaattaaa gcccatttct ttaaaatatt
//
LOCUS       DAOMTA010000169         4345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_595_length_4345_cov_1.933800, whole genome shotgun sequence.
ACCESSION   DAOMTA010000169 DAOMTA010000000
VERSION     DAOMTA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_595_length_4345_cov_1.933800"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..615)
                     /locus_tag="PLK68_05885"
     CDS             complement(52..615)
                     /locus_tag="PLK68_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89976.1"
                     /translation="MFRKIMKNKSIFSYLLVVMCILVLIETTILVGSLSTGGMFAKLN
                     QNAKDIVDQRVINRSSYLQNEMLNNWSNLSQLTDHINTTAKQLVSEGKVDYEHLDDSS
                     ETATPLILAVVDQLISTMRSQHVTGAYIIFNNHDLDKGLEDKPGIYLRDLDPLSKASA
                     ENGDLLIERAPTEVVKSLKYFINLGLD"
     gene            complement(637..>1599)
                     /locus_tag="PLK68_05890"
     CDS             complement(637..>1599)
                     /locus_tag="PLK68_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM89977.1"
                     /translation="VELFMLNKTDWEKFANATGASTNDLNTIEGVTKVAEQYYNWTDS
                     LTAAPNDGKAFFGRDAFANYMLVGYRQLATDIFSKKDNKIVLNFEADIAKKLWDNYYV
                     PYISGYFSSSGKFRSDDIKIGNILACVSSSSSVTYFPKEVILNDEESHSIELETFACP
                     KFKDGKDYVVQQGAGMVVLKSEEKEQQAAVEFLKWFTSDKQNIAFSNASGYLPVTKSA
                     NDLDKITDEVEVNESVKKTLNTSLKMISDNNMYTSVPFEKGTDARNVLETTMSNLAKQ
                     DRETVITNLSNGMNLEQAISQFNNDTYFNQWYTNTKSQLESLIK"
     assembly_gap    1600..1613
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1614..2080)
                     /locus_tag="PLK68_05895"
     CDS             complement(<1614..2080)
                     /locus_tag="PLK68_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM89978.1"
                     /translation="MKKVRLVLISLLMFFAVTGCSNEDKNLLDKDDPTTIQVWHYYNG
                     AQQEEFNRLVDEFNKTVGKEKGIIVEGSGQGTISDLERNVLDSINGKAGAADIPNIFA
                     AYGDTAYQVDKLGYAVDLNKYFSKDELSKYVDGYLEEGHFSSKDTLKIFPVAKS"
     gene            complement(2184..3980)
                     /locus_tag="PLK68_05900"
     CDS             complement(2184..3980)
                     /locus_tag="PLK68_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89979.1"
                     /translation="MARINGTPRPKNLKKTLKAMASYLTRHIGTLIIIAILVLISAGA
                     NILGTYLLKPVINDYILPGNNAGLLQAIITMAIMYLTGVGACHLYNQLMVKTAQEIVK
                     EIREDLFDKIQNLPLSFFDQRTHGELMSRFTSDIDTILEALNNSFAMLIQSFIMIVGT
                     ITMIIILNPYLSIIVIASMVMMFIFIRYSSKKSKQYFNQQQKYMGSLNGFIEEMVEGS
                     KVVKVFNHEQVNFAEFEKRNQALRQAATKAVTYSGTMIPTVVSISYLNYALSAVIGGF
                     MAIYGIMDLGSLSSYLVFVRQTAMPVNQFSQQMNFILAAMSGAERIFEIMDEKVEIDE
                     GSVTLTNVRNEYGKLVECHENTGLWAWRHPRSNGDVELVLLKNLNLYAKPGQKIAFVG
                     STGAGKTTITNLINRFYELDSGSITYDGIDIKLIKKAALRQSLSMVLQDTHLFTGTIE
                     DNIRYGKLDASDEEVVAAAKLANADSFIRRLPQGYQTMLTGDGSNLSQGQRQLLAIAR
                     AAIANPPVLILDEATSSIDTHTEKLIEKGMDSLMKDRTVFVIAHRLSTVRNSKAIMVL
                     DHGEIIERGNHDELINQKGRYYQLYTGKMELS"
     gene            complement(3982..>4345)
                     /locus_tag="PLK68_05905"
     CDS             complement(3982..>4345)
                     /locus_tag="PLK68_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM89980.1"
                     /translation="DALPKGINTDLGQGGVNVSGGQKQRLCIARALLKKPKILILDDS
                     TSALDTATERKLTDGLAYYLPKTTKIIISQRLSSLAHADKIVILTDGKIDDIGTPEEL
                     ANRNHIYQDLCKIQEGDK"
BASE COUNT         1400 a          834 c          556 g         1541 t
ORIGIN      
        1 ctttattcgg gcttaatcgc cgcttttatc attaattatt taattaaaaa gctaatccag
       61 ccctaaatta ataaaatatt ttaaagattt aaccacttca gttggagctc gttcaattaa
      121 taaatcccca ttttctgctg aagcttttga taatggatca agatctctta aatatattcc
      181 aggtttatct tcaagtccct tatccaagtc atgattatta aaaatgatat atgcccctgt
      241 aacatgctga gagcgcattg ttgaaatcaa ttgatctaca actgccaaaa taagtggtgt
      301 tgctgtttca gagctgtcat caagatgctc ataatcaacc tttccttcac taacaagctg
      361 cttagctgtc gtatttatgt ggtctgtcaa ttgagataaa ttggaccaat tgtttaacat
      421 ttcattttga agataactgc ttcgattaat aactcgctga tcaacaatat ctttagcgtt
      481 ctggtttaat ttagcaaaca ttccaccagt tgaaaggcta ccaacaagga ttgttgtctc
      541 gattaacact aaaatacaca ttactacaag taaatatgaa aaaattgatt tgtttttcat
      601 gattttacga aacatcacaa tcctccttct atagaactat ttaattaatg attctaattg
      661 gcttttagta ttagtatacc actgattaaa ataagtatca ttattaaatt ggctaattgc
      721 ttgttctaaa ttcattccat ttgaaagatt agtaataact gtttcacgat cttgtttagc
      781 taaattactc attgttgttt ctaaaacatt tcttgcatca gttccctttt caaaaggaac
      841 tgatgtatac atattattat ctgaaatcat tttaagacta gtatttaacg ttttctttac
      901 tgactcattg acttctactt catcagtaat cttatcaaga tcatttgcac ttttggtaac
      961 tggcaaataa ccactagcat ttgaaaaagc aatattttgt ttatcactag taaaccattt
     1021 caaaaattca acagctgcct gttgttcttt ttcttcactt ttgagaacaa ccattcctgc
     1081 tccttgttga acgacataat ctttaccatc tttaaattta gggcaagcaa atgtctccaa
     1141 ttcaattgaa tggctttctt cgtcatttaa aataacttct ttaggaaaat atgtgacact
     1201 cgaagatgaa gaaacacaag caagaatatt tccgattttt atatcatcac ttctaaattt
     1261 acctgatgaa ctaaaataac cacttatgta aggaacataa taattatccc ataatttttt
     1321 agcaatatct gcttcaaaat ttaatacgat tttattatcc tttttactaa aaatatctgt
     1381 tgctagctgg cgatagccaa cgagcatata atttgcaaaa gcatcacgac caaaaaatgc
     1441 ttttccatca tttggagcag ctgtcaaact atcagtccag ttataatatt gctctgcaac
     1501 cttagtaaca ccctcaattg tatttaaatc atttgttgaa gcacccgtag catttgcaaa
     1561 cttctcccaa tcggttttat tcaacataaa taactctacn nnnnnnnnnn nnngatttag
     1621 ccactggaaa aatttttaat gtatctttac ttgaaaagtg tccttcttca agataaccat
     1681 cgacatactt agatagctcg tctttactaa aatatttatt taaatctact gcataaccta
     1741 acttatccac ttgataagct gtgtcaccat acgctgcaaa aatattcgga atatctgcag
     1801 ccccagcttt tccattaatt gaatctaaga catttctttc taaatcagaa attgttcctt
     1861 gaccagagcc ctcaacaatt atcccttttt ctttaccgac tgttttgtta aattcatcga
     1921 ctagacgatt aaattcctct tgttgcgcac cattataata atgccatact tgaatcgtcg
     1981 ttggatcatc cttgtctaat aagtttttat cctcattact acatccagtt acagcaaaaa
     2041 acattaataa agatatcaaa actaatctta cttttttcat atgcttatct ccattccctt
     2101 atcccctaaa ggataacaca aattggtcat ttatacaatc ttcacacttt atcaatacaa
     2161 aaaaggatac ctattgatat cctctatgac aactccattt ttccagtata caattgataa
     2221 tatcttcctt tttgattaat taattcatca tgattcccac gttctatgat ttccccatga
     2281 tcgagtacca tgattgcttt tgaattacga actgtagaaa gacgatgtgc aattacaaaa
     2341 acggtacgat ctttcattaa actatccatc cctttttcaa tcaatttttc agtatgagta
     2401 tcaatagaac ttgtagcctc atccaaaatc aaaactgggg gattagcaat tgctgcacga
     2461 gcgatagcta atagctgacg ttgtccttgt gataagttag aaccatcacc ggttaacatt
     2521 gtttgatagc cttgcggaag tcgccgaata aatgaatctg catttgctag tttagctgct
     2581 gccaccactt cttcatcact agcatctaat ttaccgtaac gtatattatc ttcaatcgtt
     2641 cctgtaaata aatgagtgtc ttgcaaaacc attgataacg attgtcgcaa tgctgctttt
     2701 tttattaatt taatatcaat tccatcgtat gttatactgc cagaatctaa ttcataaaaa
     2761 cgattaatca aattagtaat agttgttttc ccagctcctg tagagcccac aaatgcaatt
     2821 ttttgacctg gttttgcata taagtttaaa ttttttaaca aaaccagttc tacatcacca
     2881 ttagaacggg gatgacgcca agcccaaagt ccagtatttt catgacactc aactagctta
     2941 ccatattcat tgcgaacatt tgttaaagtg acacttcctt catctatttc aactttttca
     3001 tccataattt caaaaatacg ttctgcacct gacattgctg ccaaaataaa attcatttgt
     3061 tgggaaaatt gatttactgg catcgcagtt tgtcgaacaa aaaccaaata tgatgataaa
     3121 cttcctaaat ccataatccc ataaattgcc ataaacccgc ctattacagc agataatgca
     3181 taattcaaat atgaaatact gacaactgtt ggtatcatag ttcctgaata agtaactgct
     3241 ttcgtagctg cttgtcgtaa agcttgattt cttttttcaa actcggcaaa gttcacctgt
     3301 tcatgattga aaactttaac tactttactt ccttcgacca tttcttcaat aaaaccattt
     3361 aaacttccca tatatttttg ttgttgatta aaatactgct tactcttttt actactataa
     3421 cgaataaaaa taaacatcat taccattgaa gcaattacaa tgattgatag ataaggattt
     3481 aaaataataa tcatcgtaat tgttcctaca atcattataa aactttgaat caacattgca
     3541 aaactattat ttaaagcttc taaaattgta tcaatatcac tagtaaatcg actcatcaac
     3601 tcaccatgag tacgctgatc aaaaaaacta agcggtaagt tttgtatctt atcaaataaa
     3661 tcttcacgaa tttctttaac gatttcttga gcagttttta ccattaattg attatataga
     3721 tgacaagccc cgacaccagt taaatacata attgccatcg ttattattgc ttgtaatagt
     3781 ccagcattat tacctggtag aatgtaatcg ttaataaccg gttttaataa ataagtacct
     3841 aaaatatttg cacctgcact aattaatact aaaatagcaa tgattatgag tgttcctata
     3901 tgtcgagtta aataactagc cattgctttt aaagttttct ttaaattttt agggcgtgga
     3961 gttccgttta ttctagccat attatttatc tccttcttga attttacata aatcttgata
     4021 gatatgatta cgatttgcta attcttcagg tgttcctata tcatcaattt taccatcagt
     4081 taaaataaca attttatctg catgggcaag tgatgataat cgctgtgaaa taataatttt
     4141 agttgtctta ggcaaatagt atgctaatcc atctgttagt tttcgttcag ttgctgtatc
     4201 taatgcactg gtagaatcgt caagaattaa aatttttggt ttctttaata atgcacgtgc
     4261 aatacataac ctttgttttt gcccaccaga aacattaact ccaccttgcc ctaaatctgt
     4321 attgattccc ttaggcaatg catcg
//
LOCUS       DAOMTA010000170         4342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_596_length_4342_cov_2.131094, whole genome shotgun sequence.
ACCESSION   DAOMTA010000170 DAOMTA010000000
VERSION     DAOMTA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_596_length_4342_cov_2.131094"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..650
                     /gene="tilS"
                     /locus_tag="PLK68_05910"
                     /pseudo
     CDS             345..650
                     /gene="tilS"
                     /locus_tag="PLK68_05910"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
     gene            771..1304
                     /gene="hpt"
                     /locus_tag="PLK68_05915"
     CDS             771..1304
                     /gene="hpt"
                     /locus_tag="PLK68_05915"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRM89981.1"
                     /translation="MMHKDIKEILISGDQISAKCKELGAIIDKDYEGKEVLLVGLLKG
                     SVPFMAELSKYLNTDVTFDYMNVSSYEGVESKTLVVKQDLKEDVSGKNVLIVEDILDT
                     GKTLFNVKEMLLKRKANSVKIVTMLDKEEGRVFEMKADYVGFKIPNAFVVGYGLDFNE
                     RYRQLPYVGILKEDCYK"
     gene            1314..>1838
                     /locus_tag="PLK68_05920"
     CDS             1314..>1838
                     /locus_tag="PLK68_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRM89982.1"
                     /translation="MSNKNGLLKSILPWVVLLFLIGGLVTYMNQGNSKEIKYSEFVEV
                     VQDEKIKEVEIVPSSLVVDVSGKYTKKVDGKSTEYEFTTTIPNTEAEMQSLTGLLNEK
                     GIETTIVDANDRGVFMKVILSILPYVLLLGGMFFIFKMMGQGGGNAKAFDFGNSRAKL
                     EKNQTTRFADVAGAD"
     assembly_gap    1839..1848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1849..3493
                     /gene="ftsH"
                     /locus_tag="PLK68_05925"
     CDS             <1849..3493
                     /gene="ftsH"
                     /locus_tag="PLK68_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM89983.1"
                     /translation="TPVQIKESLTRDQFRLYQLIWKRFAASRMMPAQYESTSVKIAAG
                     QYRFSVSTSRCVFDGFRSVYTEADEEKEELKELVAFLKNPKKFAQMGARIPKGVLLVG
                     PPGTGKTLLARAVSGEASVPYYSISGSEFVEMFVGVGAGRVRDMFKKAKQTAPCIIFI
                     DEIDAVGRQRGTGMGGGHDEREQTLNQLLVEMDGFSGNEGIIILAATNRADVLDPALL
                     RPGRFDRQIQVANPDKNARTEILKVHARNKKFAPDVDFSNIAQRTPGFSGAELENVLN
                     EAALLAVREDHKVISMDDIDEAVDRVMGGPAKKSRKYSEKERRLVAYHEAGHAVLGLT
                     LEAANKVQKVTIVPRGQAGGYNLMTPKEETYFQTKTQLEANIAGFMGGRVAEEIFFGD
                     VSSGAHNDIEQATRIARMMVTELGMSELGPIKYDSEQGNVFLGRDYTQHNNSHSGQIA
                     YEIDVQVRKIIDECYAQAKEIIEANKDKLVIIADALLEYETLAGEQIEALFNTGKMLD
                     RHDGTFDSSSNDDSSNDSNASSTTTPSFDDADDLLDDMK"
     gene            3633..>4342
                     /locus_tag="PLK68_05930"
     CDS             3633..>4342
                     /locus_tag="PLK68_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRM89984.1"
                     /translation="MKDYLVRGLVNSKNCRVFACKTTNLVDEARKHHNLWPTASAAIG
                     RMMSATLMMAKMNKNQEKMTVVINGGGPIGTMMTVTNGDGNIKGFVANPEVHYTYNDT
                     GKLAVGVAVGHEGTLQVIRDMGLKEPFTGSVPLQTGEIGDDFGYYFAVSEQTPSVVSV
                     GVLVNDTNEVLASGGFIIQLLPEAIEEDIDYIEKVVAQCPPVSKLINEGKSPEEILKQ
                     LFDDVEITEKQDLFFKCD"
BASE COUNT         1481 a          553 c          932 g         1366 t
ORIGIN      
        1 ataatgttga atatcgtgat gattatacaa attttcaaac tgagtttact cgtgattatg
       61 ttcgaaatgt tactttgaaa gattatgatg aagaaaaaat agagaaatta cttaagtggg
      121 cacaagaaca taatcaaaga tatgctagta agctaaaaca attacaaatt tatcttgatt
      181 tatatcatca aaaaaataaa attgattata cttgtattcc taaagagctg ttagatggat
      241 ttatttatga aattttaaaa gaagttgttt atcccccttt gatcagcaat gctttgatta
      301 aagaaattat taaacaaata aaaagtaata agccaaatat taatatggat ttgcctgtta
      361 atattaggtt cataaaagag tatgataata tatgtgtttc taatttgaaa aataaccata
      421 gctattgttt aaaatatgaa catttagttt atgataagca tgagcacttt tatttaagtg
      481 aagtaggtca tttaaatgaa ggcgtctatg tgagtaaaga agatttccca attacgatta
      541 gatcggcacg tcctggtgat gttattgtta ctgctggtgg aacaaaaaag gtttcgcgat
      601 tgtttattga caataaaatt cctaaaagta agcgggatac ttggccaatt gttgaaaata
      661 gccaaggaat gataattctt gtgccacatc ttgcaaaaaa tattgggtat ttatattcaa
      721 aacccaatat atatgtggta aaattagaaa catatacgac taggagtgag ataatgcata
      781 aggatataaa agaaattttg atcagtggag atcaaattag tgctaaatgt aaagaattag
      841 gggcaatcat tgataaagac tatgagggga aagaagtcct attagttggt ttattaaaag
      901 gttcagtccc ttttatggcg gagttatcaa aatatttaaa tactgatgtt actttcgact
      961 atatgaatgt aagtagttat gagggtgttg aatccaagac attagttgtt aagcaagatt
     1021 taaaggaaga tgtcagtggt aaaaatgtct tgatcgttga agatatttta gatactggaa
     1081 agacgttatt taatgttaaa gagatgctat taaaacgtaa agccaatagt gttaaaattg
     1141 tcactatgtt agataaagaa gagggtcggg tttttgaaat gaaggccgat tatgtgggat
     1201 ttaaaatccc taatgctttt gtagtaggct atggacttga ctttaatgaa agatatcgtc
     1261 aattgccata tgttggaata ttaaaagaag attgttataa gtaaggagag tatatgagta
     1321 ataagaatgg attattaaaa tcgattcttc catgggtagt tctattattc ttaatcggtg
     1381 gacttgttac ctatatgaat cagggtaata gtaaagagat taagtatagt gaattcgtgg
     1441 aagtagtcca agacgaaaaa attaaggaag tagaaattgt tccttcaagt ttagtcgttg
     1501 atgtaagtgg gaagtataca aagaaagtag atggtaaatc aacagagtat gaatttacaa
     1561 ctacgattcc taatactgaa gctgaaatgc aatcattaac tggattgtta aatgaaaagg
     1621 gtattgaaac tacgattgtt gatgctaatg atcgtggtgt atttatgaag gtcattctta
     1681 gtattttacc atatgtatta ttacttggag gaatgttctt tatcttcaag atgatgggtc
     1741 aaggtggcgg aaatgctaaa gcctttgatt ttggtaactc tagagctaag ttagaaaaaa
     1801 accaaacaac tagatttgct gacgtagctg gtgctgatnn nnnnnnnnta cacctgtaca
     1861 gataaaagaa tcccttacac gagatcagtt ccgtttgtat caactgatat ggaaacgttt
     1921 tgcggcaagc cgtatgatgc cggcgcaata tgaatctact tcggttaaga ttgcagccgg
     1981 gcagtatcgt ttttcggttt cgacatcaag atgtgtattt gacgggttta gatctgtcta
     2041 tacagaagct gatgaagaaa aagaggaatt aaaagaatta gtagcattct tgaaaaatcc
     2101 gaaaaagttc gctcaaatgg gggctcgtat tccaaaaggg gtattacttg tggggccacc
     2161 aggtactggt aaaactttat tagcaagagc cgtttctggt gaggctagtg tgccttatta
     2221 ttcaatttct ggttcagaat tcgtagaaat gttcgttggt gtcggtgctg gtcgtgttcg
     2281 tgatatgttc aaaaaggcca aacaaactgc gccatgtatt atctttatcg atgaaattga
     2341 tgctgttggg cgtcaaagag gtactggtat gggtggtgga cacgatgaac gtgaacaaac
     2401 tcttaaccaa ttgttagttg agatggatgg atttagtggt aatgaaggga tcatcatttt
     2461 agcggcaaca aaccgtgctg atgtattaga tccagcatta ttacgaccag gtcgttttga
     2521 ccgtcaaatt caggtagcta atcctgataa gaatgctcgt actgaaatct taaaagttca
     2581 tgcgcggaac aaaaaatttg ctcccgatgt agattttagt aatatagcac aaagaactcc
     2641 tggattctca ggggctgaac tagaaaatgt tttaaatgaa gctgctttat tagcagttcg
     2701 tgaagatcac aaagtaatct caatggatga tattgatgaa gcagttgacc gtgttatggg
     2761 tggacctgcg aaaaaatctc gtaaatatag tgaaaaagaa cgccgtctag ttgcatacca
     2821 tgaagctgga catgctgtgt taggtttaac tttagaagct gccaacaaag ttcaaaaagt
     2881 aactattgtg ccacgtggcc aagctggggg ttataactta atgacgccta aagaagaaac
     2941 atatttccaa actaagactc aattagaagc taatattgct ggatttatgg gtggtcgtgt
     3001 agctgaagaa attttctttg gtgatgtaag ttccggtgct cataacgata ttgaacaagc
     3061 aactagaatt gcaagaatga tggttactga attgggaatg tcagaacttg gtccaatcaa
     3121 atatgattct gaacaaggaa atgttttctt gggacgcgac tatacacaac acaataattc
     3181 tcattctggg caaattgctt atgaaattga tgttcaagtt cgtaagatca tcgatgaatg
     3241 ctatgcacag gcaaaagaaa ttattgaggc taataaagat aaattagtaa ttattgctga
     3301 tgctttatta gaatatgaaa cattagctgg tgaacaaatt gaagcattat ttaatacagg
     3361 taagatgtta gatcgtcatg atggaacatt tgatagtagc tctaatgatg attcatcaaa
     3421 tgattctaat gcttcaagta caacaacacc atcgtttgat gatgctgatg atctattaga
     3481 tgatatgaaa taaaagcggt atccgctttt tttcatatgt gaggtaaatg atgaacaaca
     3541 agaaaagaaa aatatttatc tattttatta ttttgtgtat gataatacca atgatattat
     3601 catcattgat ggtattttta taaggagaat atatgaaaga ttatttagtt agaggtttag
     3661 ttaatagtaa aaactgtcgc gtttttgctt gtaaaacgac taatttagta gatgaggctc
     3721 gcaaacatca taatttatgg cctactgctt ctgcagcaat cgggagaatg atgtcagcta
     3781 ctttgatgat ggcaaaaatg aacaagaatc aagagaaaat gacagttgtg atcaatggtg
     3841 gcggtccaat tggaactatg atgacggtta ctaatggtga tggaaatatt aaaggattcg
     3901 ttgctaaccc agaagtgcat tatacttata atgatactgg taaattagca gttggtgtag
     3961 cagttggaca tgaaggaact ttacaagtaa ttcgtgatat gggtttaaaa gaaccgttta
     4021 cgggatcagt tccactacaa actggggaaa taggtgatga ttttggttac tactttgcag
     4081 ttagtgaaca aactccttca gttgtatcgg ttggggtact agtaaatgat actaatgaag
     4141 tgcttgcttc aggtggattt attattcaat tattacctga ggctattgaa gaagatattg
     4201 attatatcga aaaagttgtc gctcaatgtc cgccggtatc taagttgatc aatgaaggga
     4261 aaagtcctga agagatatta aagcagctat ttgatgatgt ggagattact gaaaaacaag
     4321 atttgttttt caaatgtgat tg
//
LOCUS       DAOMTA010000171         4337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_598_length_4337_cov_2.357076, whole genome shotgun sequence.
ACCESSION   DAOMTA010000171 DAOMTA010000000
VERSION     DAOMTA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_4337_cov_2.357076"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="PLK68_05935"
     CDS             <1..1899
                     /locus_tag="PLK68_05935"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM89985.1"
                     /translation="ISGKPKLDKLPATYVESPDEATTLLITLYDNIIDSKIILSYTIF
                     ENMPVITRNAYIENCGEQKITLEQVMSMSLDLPDKEYEMIELTGAWARERSIKERKLE
                     HGIQSIYSLRGCSSNNFNPFIALKRENCNEYAGEVLGFSFVYSGNFLAQVEVDTYDVT
                     RVMMGIHPHCFSWSLEKGESFQTPEVVMVYSNDGLNKMSQTYHKLYQRRLARGKYRDQ
                     VRPILVNNWEATYFDFDEEKIMDIAGTAQKLGIELFVLDDGWFGKRNNDVAGLGDWYP
                     NLQKLPSGISGLSRKINNLGMKFGLWFEPEMVNKNSDLYRNHPQWILETPNRPSSHGR
                     NQFVLDFSNPEVVIYVYNMMTEVIDKANISYIKWDMNRCMTEVYSSCHDSESQGRVMH
                     EYILGVYQLYELLTSRFPEILFESCASGGARFDPGMMYYAPQCWTSDDTDALERLKIQ
                     YGTSMVYPVSSLGAHVSAAPNHQLLRNTPIETRANVAYFGTFGYELDLNKLSMDEQIK
                     VKEQITFMKDYREIIQFGTFYRLKSPFEGNETVWMVVSQDQNTAIIGYYRTLQEVNVG
                     YRRVKLLGLDPDKEYHVNLNNTVHYGDELMNLGLITTDSSCGENKEKYDGTNGDYLSR
                     IYVLKAKI"
     gene            complement(2181..2858)
                     /locus_tag="PLK68_05940"
     CDS             complement(2181..2858)
                     /locus_tag="PLK68_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRM89986.1"
                     /translation="MKYVCKICGFIYDEATQDAKWNDLDTDWVCPVCKAPKEKFEAVA
                     ETSTSKYARTKTEKNLMEAFAGESMARNKYTFFAEAARKEGLEQIAAIFEETADQEKQ
                     HAKMWFAAFHGIGTTDENLEAAAAGENEEWTEMYARMAQEAREEGFEELAIKFENVAI
                     VEKSHEKRYLKLLESYKNGTTFKGDAPKGWKCRQCGFIYEGDEAPEHCPTCGYPKAFF
                     ERMCENY"
     gene            complement(3608..>4337)
                     /locus_tag="PLK68_05945"
     CDS             complement(3608..>4337)
                     /locus_tag="PLK68_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM89987.1"
                     /translation="GQGEYHVNNQVYQIKAGDAFLIMPNVVTYYQADQHEPWTYLWIS
                     FDGSKTRQYLKRCNLDEHNLVVHCDYINELRETTIAILAHNKLSYSNELFIQGQLYTF
                     FSFLAKSANITYNDNVAPNYNPYVDKAIEYIQNNYQEMVTVNEIADYLSLNRSYLTTL
                     FKKHLHLSPQEFLLKYRMMRAEDLLTNTDLTINQIAFSCGYSNQLSFSKAFSNSHQMA
                     PRDYRKQFKLVDNSRMNDPHERKE"
BASE COUNT         1413 a          608 c          763 g         1553 t
ORIGIN      
        1 ataagtggta agcctaaatt agataaatta cccgcgacat atgttgaatc accagatgaa
       61 gcaacaactt tattaataac tttatatgat aatataattg attcaaaaat tattttaagt
      121 tacacaattt ttgaaaatat gccggtaatt acaagaaatg cttacattga aaattgcggt
      181 gaacaaaaaa taaccttaga gcaagtgatg agtatgtcac ttgatttacc cgacaaagaa
      241 tatgagatga ttgaattaac aggtgcttgg gctcgtgaaa ggagcattaa ggagagaaag
      301 ctagagcatg gaattcaatc tatatattct ctaagaggat gttcaagtaa taattttaat
      361 cctttcatcg ctttaaagcg tgaaaattgt aatgaatatg ctggtgaggt tttaggattt
      421 agttttgtat atagtgggaa ttttttagct caggttgaag ttgatactta tgatgttaca
      481 agagtgatga tgggaataca tccccactgt tttagctgga gtcttgaaaa aggagaaagt
      541 tttcaaactc cggaagtagt catggtgtat tcaaatgatg gactaaataa aatgtcacaa
      601 acttaccaca aactttatca aagacgattg gctcgtggga aatatcgtga tcaagtacga
      661 cctattttag ttaataattg ggaggcaacg tactttgatt ttgatgaaga aaaaattatg
      721 gatattgctg gaactgctca aaaattagga atcgagttat ttgttcttga tgatggctgg
      781 tttggtaaac gtaataatga tgttgcaggg ttaggtgact ggtatccgaa tttacaaaaa
      841 ttaccaagtg gaattagtgg tttatcaaga aaaataaata atctgggaat gaagtttggt
      901 ttgtggtttg aaccagaaat ggtcaataaa aattctgatt tatatcgtaa tcatccacag
      961 tggattttag aaacaccaaa ccgtcctagt agtcatggac gtaatcaatt tgtattagat
     1021 ttttcaaatc cagaagtcgt tatttatgtt tataatatga tgacagaagt aattgataaa
     1081 gcaaatattt cttatattaa atgggatatg aaccgctgta tgacagaagt ttactctagt
     1141 tgtcacgata gtgagagtca gggacgagtt atgcatgaat atattttagg agtgtatcag
     1201 ttatatgaat tgttaacaag tcgttttcct gaaattttat ttgaatcttg tgctagtggt
     1261 ggtgctcgtt ttgatccagg aatgatgtat tatgcaccac aatgttggac cagtgatgat
     1321 actgatgcgc tagaacgttt gaaaatccaa tatggaacat caatggtata tcctgtttcg
     1381 agtcttggag ctcatgtatc ggcagcgcca aatcatcagt tgttgagaaa tacaccgatt
     1441 gaaacaaggg caaatgttgc ttattttggt acttttggat acgaattaga tcttaataaa
     1501 ttaagcatgg atgaacagat aaaagttaaa gaacaaatca cctttatgaa ggactatcgg
     1561 gagattattc agtttggaac gttttaccgt ttaaaaagtc catttgaagg taatgagact
     1621 gtttggatgg ttgtatcaca ggatcagaat actgctatta ttggatatta tcgtactttg
     1681 caggaagtta atgttggtta ccgtcgtgta aagctgttgg gattagatcc ggataaagaa
     1741 tatcatgtta atcttaataa tacagttcat tatggtgatg aattaatgaa cttgggttta
     1801 attacaactg atagttcttg cggtgaaaat aaagaaaaat acgatgggac taatggagat
     1861 tatttatcta gaatctacgt attaaaagca aaaatataat aatcctgagt aaacctcacc
     1921 agttggataa gacaggctta agtgtagagc tattctacgg taaaccgtta ttctagcttg
     1981 tggggtattt atatatcaaa gagaactgtt tagatgagaa gtttttaatc agatttaaaa
     2041 tagtttatag gtatggattt agaatattat aatcaaaaag tcataaatta aaaggattga
     2101 tttctttata aagtaatcaa tccaaatatt taattattat atttaattac atctttttaa
     2161 aatagttata ttaattctta ttagtaattt tcacacattc tttcaaaaaa ggctttagga
     2221 tatccgcatg taggacaatg ttcaggtgct tcatctcctt cataaatgaa tccgcattga
     2281 cgacatttcc atccttttgg ggcatcgcct ttgaatgttg tgccgttttt atatgattct
     2341 aataatttta gataacgttt ctcatgggat ttttcaacga tagcaacatt ttcaaattta
     2401 attgctaatt cttcaaaacc ttcttcacgt gcttcttggg ccattcttgc atacatttca
     2461 gtccactctt cattttcacc tgcagcggct gcttctagat tttcatcagt tgttccaatt
     2521 ccatgaaatg cggcaaacca cattttagca tgttgttttt cttgatcagc tgtttcttca
     2581 aaaattgcag caatttgctc taatccttct ttacgagctg cttcagcaaa gaaagtatat
     2641 ttattacgag ccattgattc accagcaaag gcttccatta aattcttttc agtttttgtt
     2701 ctcgcatatt tactagtact agtttctgct actgcttcaa atttttcttt tggagcttta
     2761 caaaccggac atacccaatc agtatctaaa tcattccact tagcatcttg agtagcttca
     2821 tcataaataa atccacaaat tttacaaacg tatttcatct tcttttcctc caaatttatt
     2881 aatttatgaa tctttatttg atatatgttt aaattagctt ttttcattac aaggaatttt
     2941 ttagtagttt attaattaaa tctttctcac gtcattttta gcatttttca ataacttgtt
     3001 taaaaagttt ctagtatata aactttgagg tagcataaat cttgatttac gaacaatgtc
     3061 ttcatttatt aatcggttag atttgactag agtgttagct atttgcttaa taacaggatt
     3121 gcttgtttgt tttgaaagat tttgagctat tacttcttgt tttgctttac ttcaggttaa
     3181 ataacctaac tcattaagca ttttaacttc tttatttcgt ggtggatagt cataagggac
     3241 catagaaatg gcatttaatc acaggtgtgc gttaatacat tttgaagcct taatttgatc
     3301 atcttgtgta tcacttgctc tggtaggaca aacgtataaa tgaaggtaat ctttagtgta
     3361 tcggtgaata tttccttttg ctctcgtttt cacattattg gcttctaaca attttaaact
     3421 tgtcattttg taaattggac aaaattcagg tggaaagatc tagtataaac aaattcgcaa
     3481 atactactta acttttctaa ttcttttaaa tgggtaatag taatcattat taatatatta
     3541 taattgcaat tacttacatg aataaaagca ataataatta ataaaaagaa tagattgatc
     3601 taataatcta ttctttgcgt tcatgaggat cattcattct tgaattatca actaatttaa
     3661 attgtttacg ataatcacgt ggtgccattt gatgtgaatt actaaatgct tttgaaaaag
     3721 ataattggtt agaatatccg caagagaagg caatttgatt aattgtaaga tcagtattag
     3781 taagaagatc ttcggcgcgc atcattcgat actttaataa aaattcttgt ggtgataaat
     3841 gaagatgctt tttaaataat gttgttagat aactgcggtt gagagaaagg taatcagcaa
     3901 tctcgttgac tgttaccatt tcctggtaat tattttgaat atattcaatt gctttatcaa
     3961 catatggatt gtagttaggg gctacattat cattataagt aatattagca cttttggcta
     4021 aaaaactaaa aaaggtatac agttgtcctt gaataaataa ctcgttacta tagcttagtt
     4081 tattgtgtgc aagaatagca atcgttgttt ctctcaattc attgatataa tcacaatgaa
     4141 ctacaagatt atgttcatct aaattacatc gttttaaata ttggcgagtt ttagaaccat
     4201 caaagctgat ccataaatat gtccaaggtt catgttgatc ggcttgataa taagtgacaa
     4261 cgtttggcat tattaaaaaa gcatcacctg ctttaatctg atatacttgg ttgttaacgt
     4321 gatattcacc ttgaccg
//
LOCUS       DAOMTA010000172         4330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_601_length_4330_cov_2.425965, whole genome shotgun sequence.
ACCESSION   DAOMTA010000172 DAOMTA010000000
VERSION     DAOMTA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_601_length_4330_cov_2.425965"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLK68_05950"
     CDS             <1..195
                     /locus_tag="PLK68_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89988.1"
                     /translation="AWFNVVGDILASNSSWSAVLEERSTPPSDDDNDDRQNDLDVLND
                     AIDKLKESIEKLQNEKKSND"
     gene            complement(216..641)
                     /locus_tag="PLK68_05955"
     CDS             complement(216..641)
                     /locus_tag="PLK68_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM89989.1"
                     /translation="MKKLKYFIPALIWMIFIFIMSHTNGNDSSNQSNFIAEIILKVIN
                     IDRDTLTFIIRKAAHMSEYAILLLLLYYALSNVISKHTLSLSLLVTFIYACSDEFHQL
                     FIPGRSGQFKDVLIDTSGALIMLMIIFLWQRKKKSKLIK"
     regulatory      820..913
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            966..2336
                     /locus_tag="PLK68_05960"
     CDS             966..2336
                     /locus_tag="PLK68_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRM89990.1"
                     /translation="MQFITNLLTKIDELVWGLPLICLLLGTGIYFTCKLKLLQLTKLK
                     LAFSCIFKKQDNDEGDVSSFQALCTALSSTIGTGNIVGVATAIAAGGPGALFWMWVSA
                     FFGMATKYAEGLLAIRYRIKDENNQMAGGPMYYLERGLGSKWLAKIFAFFGVAVALLG
                     IGTFTQVKSISDAMELSFNVPPIVTAILLTITVGFITIGGIKRIANVAEKVIPMMCIL
                     YIGGVVIILITHFNLIPQTVVLVVHSAFNPQAALGGGIGITMTMAMQKGISRGIFSNE
                     SGLGSAPIAAAAAKTDSCVEQGLVSMTGTFIDTVVICTMTGIAILLTNSHTSGLEGAA
                     MTTQAFSNGLFIPMIGKYIVNIGLIFFAFTTIIGWNYYGERCMYYLKGLKAIPYYKFI
                     YIVFIAIGPFMSLEFIFILADIVNGCMAIPNLIGLIGLRKEVIVQTQAYFTDEEVINQ
                     DELVYD"
     gene            complement(2557..>3918)
                     /locus_tag="PLK68_05965"
     CDS             complement(2557..>3918)
                     /locus_tag="PLK68_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM89991.1"
                     /translation="AEINITNSLELKLIVYEDITFSDLLHAIYQSSNVLHCIKFMITN
                     ESNHSFLVFAEDNYLAKSSAYRIRQKCIKYIRSIGLDIKKNKVIGEEYRIRFLIALLY
                     YKCGIDCCDIDEYSIKLIKEFIISTNQSVTFEFLNNATNEYDYFECLMSLLWKRKDHN
                     DNLSIPKELEKFKEIFIYNDLKKYLHNVIGNQLNIDFSKKDYDYMYLVYFCTNNFLFA
                     NQWTEERKEHIYKIFLSNQKFYDLYIRLSHKYGKFLEQSHIFKTILIYFFKDHLFQLQ
                     CLIPNQRLYIDTTPTTTYITNNDVSGIINSWKKANNILYPTDKRSIFYLTTQIEFILR
                     QLVPPVTIILLSDLMSEIEMINLFLEKNFSRNRINITPLLLNSYNLEYLNAEKNRIII
                     ITRGIEYLLKSYNFFQNNTIVIIDSRTTLYEKEKISNAIANYEEKQFLKICAGTSLTD
                     TEY"
     assembly_gap    3919..3928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1482 a          509 c          729 g         1600 t
ORIGIN      
        1 gcatggttta atgttgtagg tgatatttta gcctcaaact cttcttggtc agctgtctta
       61 gaagaacgct caacgccacc gagtgatgat gacaacgatg atcgtcaaaa tgatcttgat
      121 gtacttaatg atgccattga taagttaaaa gaaagtattg aaaaactgca aaatgaaaag
      181 aagtctaatg attaatcaaa ataacttaag taaagttatt tgataagttt agacttcttt
      241 tttctttgcc ataaaaagat gatcataagc atgatcaagg caccagaagt gtcaattaga
      301 acatctttaa attgtccact gcgccctgga ataaacaatt gatgaaattc atcactacat
      361 gcataaataa atgtgactaa taatgataat gataaagtat gcttagatat aacgttactt
      421 agtgcataat aaagtaagag taataagatt gcatattcac tcatatgagc ggcttttcta
      481 attataaaag ttaatgtatc acgatcaata ttaataactt ttaaaattat ttcagcaata
      541 aaattacttt gatttgaaga gtcattacca ttagtgtgtg acattataaa aataaagatc
      601 atccaaatta gggcaggaat aaaatatttt aattttttca taattgttat tatagaagag
      661 aaatgtcgtc tttacaaata ctttattgca aaaataaaaa atatttgcaa ttgtatacaa
      721 ttagcgtata atcttagtgt atatatcgga tgataatggc gaaagagatt ctaattgaac
      781 accgaagaat taaagatttc aggtaaaagg atcgctattg gacgaacctc tggagagact
      841 tattaaatta agcaccgaag gagcaaacct atttataggt gaaactctca ggtaaaagga
      901 cagtgggatg aatagttttt ttcattatta attccgactt aattatttta agaaggagtt
      961 tttttatgca atttattaca aatttactta caaaaattga tgagcttgta tggggcttac
     1021 cgcttatttg tctattgttg ggaactggaa tttatttcac atgcaagcta aaactgttgc
     1081 aattaacgaa attgaaatta gcgttttcat gtatttttaa aaaacaggat aatgatgaag
     1141 gtgatgtatc aagttttcag gctctttgta cagcactatc ttctacgatc gggacaggaa
     1201 atattgttgg agtagctacc gcgattgcgg ccggaggacc aggagcatta ttttggatgt
     1261 gggtatcagc tttttttgga atggctacaa aatatgctga aggcttatta gcaattcgtt
     1321 accgtatcaa agatgaaaat aatcaaatgg cagggggacc aatgtattat ttagaacggg
     1381 gtttaggaag taaatggtta gctaagattt ttgctttttt tggagttgct gtggcgttat
     1441 tgggaattgg aacatttact caagttaaat caatttctga tgcaatggag ttatcgttta
     1501 atgttccacc aattgttaca gcgattttat taacgatcac tgtaggtttt attacaattg
     1561 gcgggattaa aagaattgcg aatgtagcag aaaaagtaat tcctatgatg tgtattcttt
     1621 acatcggtgg tgttgtgatt attttaatta ctcattttaa cttaatcccg cagacagttg
     1681 tattagtggt tcatagtgca tttaatcctc aagcagctct tggtggtggt ataggtatta
     1741 ctatgacaat ggcaatgcaa aaaggaatta gtagaggaat tttttcaaat gaaagtggtc
     1801 ttggaagtgc accaattgcc gctgctgcag caaaaactga ttcatgtgta gaacaaggat
     1861 tggtatcaat gactggaacg tttattgata cagttgttat ttgtaccatg acaggaattg
     1921 caattttact tactaatagc catactagtg gtttagaagg tgccgcaatg actacacaag
     1981 catttagtaa tggtttattt attcctatga ttgggaagta tattgttaat attggattaa
     2041 ttttctttgc atttactact ataattggtt ggaattatta tggtgagaga tgtatgtatt
     2101 atttaaaagg tttaaaagcg attccatatt ataaatttat ttatattgtc tttattgcaa
     2161 ttggtccttt tatgtcatta gaatttattt ttattctcgc agatattgtt aatggttgta
     2221 tggctatccc taatttaatt ggtttaattg gattaagaaa agaggtcata gtacaaactc
     2281 aagcatattt tactgatgaa gaggttataa atcaagatga attagtatat gattaaaggg
     2341 gctgtaacgg attaaatgat atgcttcctt ctaggtagac aagtgaaata ataaaaaatt
     2401 gtcacttaga agggggtatg tcaaagatta aaatttttat tcgttacagt ccttttttga
     2461 tttaagtgtt tttgccaaga aataagttta agataaaata tgctatttaa ataatataat
     2521 atttgtataa atagactgtt gcacttatgt tgatcattaa tattctgtat cagtaagact
     2581 tgttccagca caaattttta gaaattgttt ttcttcataa ttggcaatag cattcgaaat
     2641 tttttctttc tcgtataagg ttgttctaga atctattatt actatagtat tattttgaaa
     2701 gaaattgtat gattttaata aatattcaat tcctcgagta atgattatta ttctattttt
     2761 ttcagcgttc agatattcta aattataact gtttaaaagt aacggagtta tattaattcg
     2821 attgcgggaa aaattttttt caagaaaaag atttatcatt tctatctcgg acattaaatc
     2881 agataacaat atgattgtta caggtggaac aagttgtctt aatataaatt caatttgtgt
     2941 tgttaaatag aatatacttc ttttatctgt tggatataat atattatttg cctttttcca
     3001 tgaatttatg atacctgata cgtcattatt ggtgatgtaa gttgtagtag gtgtagtatc
     3061 tatatataga cgctgatttg gaattaggca ttgtaattga aaaagatgat ccttaaaaaa
     3121 atagattaaa attgttttga aaatgtgaga ttgttctaga aatttaccat atttatgact
     3181 aagacgaata tataaatcat aaaatttttg attggaaaga aatattttat aaatgtgctc
     3241 tttacgttcc tctgtccact ggttggcaaa aagaaagtta ttagtacaaa aataaactaa
     3301 atacatataa tcatagtctt tttttgaaaa atctatgtta agctgattcc caattacatt
     3361 atgtaaatat tttttcaaat cattgtatat aaaaatctct ttaaattttt caagctcctt
     3421 tggtatagat aaattgtcat tgtggtcttt tcttttccac aaaagtgaca ttagacattc
     3481 aaaataatca tattcatttg tagcgttatt aagaaattca aaagtaactg actggttagt
     3541 agatataata aattctttaa taagttttat tgaatattca tcaatatcgc aacaatctat
     3601 accgcatttg tagtataaaa gagcaatcaa aaatcgaatt ctgtattctt cccctataac
     3661 tttatttttt ttaatatcca aaccgatact gcggatatat ttaatacatt tttgtcgtat
     3721 tcgatatgca ctggattttg caagatagtt atcttccgca aataccaaaa aagaatggtt
     3781 tgattcattg gttatcataa acttgataca atgcaataca ttagagcttt ggtatatagc
     3841 atggagtaaa tcagaaaatg taatatcttc gtatacaatt aatttaagtt ccaatgaatt
     3901 tgttatattt atttctgcnn nnnnnnnngg tactgctaaa tgtaagtata tacttacatt
     3961 cttttacaga agtgactctc tttaaatata agatggaaaa aagatataat ttcttttttg
     4021 tagtgttttc taaaaaatag tcaagcataa ttcaccacct acaacagaac ttaagctatt
     4081 tatagttttt acaactatat aatatgataa ttatgtgatt aaattgttcc caaattttat
     4141 tgatatagga tatatttttg ggaaaaaata ttattaaaaa tttaatatga tattaaaaag
     4201 gtatttactt gtatattgta ttgaaatttt ggagagttat tttggtttat ttgattgatg
     4261 tattttttaa ttcagtattt atatttaaaa tgtttgtgaa taaagcttga ggggataggt
     4321 tattatgcat
//
LOCUS       DAOMTA010000173         4327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_602_length_4327_cov_1.799625, whole genome shotgun sequence.
ACCESSION   DAOMTA010000173 DAOMTA010000000
VERSION     DAOMTA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_4327_cov_1.799625"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PLK68_05970"
     CDS             <1..121
                     /locus_tag="PLK68_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRM89992.1"
                     /translation="KRKFTFVDINKKVINKVVDKCNKQKINNRKIEIEIANKK"
     gene            265..2931
                     /gene="ppdK"
                     /locus_tag="PLK68_05975"
     CDS             265..2931
                     /gene="ppdK"
                     /locus_tag="PLK68_05975"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRM89993.1"
                     /translation="MKKYVYMFSEGNEMMRDLLGGKGANLAAMVNLGLPVPQGFTVTT
                     EACNEYYADGKIINDEMKKQIDDCLERLEKLADKKLGGLDNPLLVSVRSGAKFSMPGM
                     MDTILNLGLNDETVEVVAKQTGNRRFAFDSYRRFIQMYSDVVCEVDKELFEAKLSELK
                     TSKGYESDLDITAEDFENIIIPQYKEIFKTQLGRDFPQEAKEQLMGAILAVFRSWNND
                     RAIIYRNLNGIPHDLGTAVNVQQMVFGNMGDDSGTGVLFTRNAANGDNHIYGEYLMNA
                     QGEDVVAGIRTPQKIAKLEEDMPEIYKQLVTIVKGLEKHYKDMQDCEFTVENGKLYIL
                     QTRNGKRTGKAALKIAVDLVHEGLINKYEAMTRVEPDQISQLLHPNFTPAALAAAEVL
                     IEGLPASPGAGAGKVYLTAEKVHEQAVAGEKVILVRHETSPEDIQGMVDCEGILTSTG
                     GMTSHAAVVARGMGKCCIVGAKALSIDYDAGTFTIDGKTYPEGTEISLDGTSGKVYMG
                     ILDSEESELTGDFAELMSWADEIKKLQVRANADSPRDAAVAIKFGAEGIGLCRTEHMF
                     FEGDRIEYVRQMILSDTVEERIKALDELYKFQVEDFRGIYRAMVGLPVTVRLLDPPLH
                     EFLPHTDEEYQAVADKLGKTLEEVKTKGATLKETNPMLGHRGSRLAVTYPEIYNMQVR
                     AIIDAAIDVERELGCTIVPEIMLPLIGSESEIVYVKDNVTKAIDAAIMAKNAKIEYKI
                     GTMIEIPRAALTADEIAKHAEFFSFGTNDLTQMTFGFSRDDVGSFLPEYINRKVIQVD
                     PFVSLDQSGVGQLVEMAANKGRSVRPKIKLGICGEHGGDPESIKFCHKTGLTYVSCSP
                     YRVLIARLAAAQAAAEEIILEHTTDKVLVADK"
     gene            3014..>3364
                     /locus_tag="PLK68_05980"
     CDS             3014..>3364
                     /locus_tag="PLK68_05980"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRM89994.1"
                     /translation="MKNIIIVATGGTIAGSGKVGKATNYQAGKINIKEIIDSIPMINE
                     VANLKAIQLFNVDSNEMNEEHWIILANKINDLASQKNVDGIVVTHGTDTLDETAYFLN
                     LTINTYKPVVLTGAM"
     assembly_gap    3365..3374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3375..4002
                     /locus_tag="PLK68_05985"
     CDS             <3375..4002
                     /locus_tag="PLK68_05985"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRM89995.1"
                     /translation="PASATSADGPINLYQAVCLASCDDALGHGVMAVFSSTIYSGRDI
                     QKISNFKTDAFDQKDFGCLGYMNDDKVMMFSRTFKKHTLQSVFSEKPITELPPVWIVY
                     YYAGAKPDILTMMAQNHRGIVIIGSGSGNYSQAWLNEIETLGAQGIIFVRASRVNQGI
                     VYESDVFDPHNVCIPSNTLSGQKARVLLMLALSVTQNTKEIKRIFNEY"
     gene            4013..>4327
                     /locus_tag="PLK68_05990"
     CDS             4013..>4327
                     /locus_tag="PLK68_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM89996.1"
                     /translation="MCVERIKLNLDSIAMMLFCARLKAYKEVPLSNEEWLLIERIIKK
                     KGLKGPASLLSMNQTELEEILEINEFIAYKMARRIETMNIFLSVLNNLEGNGINVTTK
                     YED"
BASE COUNT         1516 a          531 c          899 g         1371 t
ORIGIN      
        1 caaacgaaaa tttacatttg ttgatattaa taagaaggta atcaataaag ttgttgataa
       61 gtgtaataag caaaagatta ataatcgaaa gattgaaatc gaaattgcaa ataaaaaata
      121 aatatttggt gaaaagattg tgttttaaaa atacaatctt tttttgtata aaaatatggt
      181 attttaaaag cgcttacact tgaatgaaaa ggctaacacg tgtataatat atttatgtat
      241 taattctaag gaggattaga ttgaatgaaa aaatacgttt atatgtttag cgaaggaaac
      301 gaaatgatgc gtgaccttct aggtggtaaa ggggctaact tggctgcaat ggttaactta
      361 gggttaccag taccacaagg atttacagtg actactgaag catgtaatga atattatgct
      421 gatggtaaga tcatcaatga tgaaatgaaa aaacaaattg atgactgctt agaaagatta
      481 gaaaaattag ctgataaaaa acttggggga ttagataatc cgttattagt ttcagttcgt
      541 tctggagcaa aattttctat gcctggaatg atggatacaa ttcttaactt aggtttaaat
      601 gatgaaactg ttgaagttgt agctaaacaa acaggaaatc gtcgttttgc ttttgatagt
      661 tatcgtagat ttattcaaat gtactctgat gtagtttgtg aagttgataa agagttattt
      721 gaagctaaat taagtgaatt aaaaacttca aagggatatg aaagtgattt agatatcact
      781 gcagaagatt ttgaaaatat cattattcct caatataaag aaattttcaa aactcaatta
      841 ggtagagact ttccacaaga agctaaagaa caattaatgg gagcaatttt agctgtattc
      901 agatcatgga ataatgatcg tgctattatt tatcgtaact taaatggaat tcctcatgat
      961 ttgggaactg ctgttaacgt acaacaaatg gtatttggta atatgggtga tgactcaggt
     1021 actggggtat tatttactcg taatgctgct aatggggata atcatattta tggtgagtat
     1081 ttaatgaacg cacaaggtga agacgttgtt gctggtattc gtacaccaca aaaaattgct
     1141 aaattagaag aagatatgcc agaaatctat aaacaattag ttacaattgt taaaggatta
     1201 gaaaaacatt ataaagatat gcaagactgt gaatttacag ttgagaatgg taaactatat
     1261 attttacaaa ctcgaaatgg taaacgtact ggtaaagcag cattaaaaat tgctgtagat
     1321 ttagtacatg aaggtttaat caataaatat gaagcaatga caagagttga acctgatcaa
     1381 atctcacaac tattacatcc gaactttaca cctgctgcat tagctgctgc tgaagtgttg
     1441 attgaaggat tacctgcttc accaggtgct ggtgctggta aagtttattt aactgctgaa
     1501 aaagttcatg aacaagctgt agctggtgaa aaagttattt tagtaagaca cgaaacttct
     1561 cctgaagata ttcaaggtat ggttgattgt gaaggtattt taacttctac tggaggaatg
     1621 acgagtcatg cagctgtagt tgctcgtggt atgggtaaat gctgtatcgt aggtgcaaaa
     1681 gcattatcga ttgattatga tgcaggaaca tttacaattg atggaaaaac ttatccagaa
     1741 ggaacagaaa tttcattaga tggtacaagt ggtaaagtat atatgggaat tttagattct
     1801 gaagaatctg aattaacagg tgattttgct gaattgatgt cttgggctga tgaaatcaag
     1861 aaattacaag ttcgtgctaa tgctgatagc cctcgtgatg cggctgtagc tattaaattt
     1921 ggagctgaag gtattggttt atgtcgtaca gaacatatgt tctttgaagg agatcgtatc
     1981 gaatatgtaa gacaaatgat tttatctgat acagtggaag aaagaattaa ggcattagat
     2041 gagttatata aattccaagt tgaagacttc agaggaatct atcgtgcgat ggttggatta
     2101 ccggtaacag tacgtttatt agatccacct cttcatgaat ttttaccaca tactgatgaa
     2161 gaatatcaag cagttgctga taaacttgga aaaacattag aagaagtgaa aactaagggc
     2221 gcaactttaa aagaaactaa tccaatgtta ggacatcgtg gttcacgttt agcagtaacg
     2281 tatccagaaa tctataatat gcaagttaga gcaattattg atgcagcgat tgatgttgaa
     2341 agagaattag gatgtacaat cgtaccagaa atcatgttac cattgattgg tagtgaatct
     2401 gaaatcgttt atgtgaaaga taatgttaca aaagcaattg atgccgcaat tatggctaaa
     2461 aatgcaaaaa ttgaatataa gatcggtaca atgattgaaa ttccaagagc tgctttaaca
     2521 gctgatgaaa ttgcaaaaca tgctgaattt ttctcatttg gaactaacga tttaactcaa
     2581 atgacatttg gtttctctcg tgatgatgta ggaagtttct tgcctgaata tattaatcgt
     2641 aaagtaattc aagttgatcc attcgtatcg cttgatcaaa gtggtgttgg tcaattagta
     2701 gaaatggctg caaacaaagg tcgcagtgtt cgtccaaaaa ttaaattagg tatttgtggt
     2761 gaacatggtg gagatccaga atcaattaaa ttctgtcata aaacaggttt aacttatgta
     2821 tcttgttctc catatcgtgt gttaattgca cgtttagctg ctgctcaagc tgctgctgag
     2881 gaaatcatcc tagaacacac tactgataaa gtattagttg ctgataaata gctgataaac
     2941 gtctcttaat ttaaagagac gtttttttat ttgtgtaaag taggatattt ggtataatag
     3001 atatggtgat aaaatgaaaa atattataat tgtagcaaca ggtggaacaa ttgcaggaag
     3061 tggcaaagtt ggaaaagcaa ctaattatca ggctggaaaa atcaatatta aggaaataat
     3121 tgactctatt ccaatgatta atgaagttgc taatttaaaa gcaattcagc tttttaatgt
     3181 cgatagtaat gagatgaatg aagaacattg gataatttta gctaataaaa taaatgattt
     3241 ggcttctcaa aaaaatgttg atggtattgt agttactcat ggaactgata cattggatga
     3301 gacagcctat tttttaaatc ttacaatcaa tacatataag ccagtcgttt tgacaggggc
     3361 aatgnnnnnn nnnnaccagc gagtgcaact agtgcagatg gtccgattaa tttatatcaa
     3421 gctgtttgtt tggcttcgtg tgatgatgct ttgggacatg gcgtaatggc agtcttttca
     3481 agtacaattt attcagggcg agatatccaa aaaattagta attttaaaac tgatgcattt
     3541 gatcaaaaag attttggatg tttgggttat atgaatgatg ataaagttat gatgttttca
     3601 agaactttta aaaaacatac attgcaatca gtttttagtg aaaagccaat tactgaattg
     3661 cctccggtat ggattgtcta ttactatgct ggtgctaaac ctgatatttt gacgatgatg
     3721 gctcagaatc atcgagggat tgtgataatt ggcagcggta gtggtaatta tagtcaagca
     3781 tggctgaatg aaattgaaac actaggtgct caaggaatca tttttgttag agcgtctcgg
     3841 gttaatcagg gaattgtata tgagagtgat gtttttgatc ctcataatgt ctgtataccg
     3901 tctaatactt tgtcaggaca aaaagctaga gtgcttctaa tgcttgcttt aagtgttact
     3961 cagaatacaa aagaaattaa aagaattttt aatgaatatt aaaggggggc acatgtgcgt
     4021 ggaacgaatc aaacttaatt tagatagtat tgcaatgatg ctgttttgtg ctcgattaaa
     4081 agcgtataaa gaggtcccgc tttctaatga ggaatggttg ttaattgagc ggattataaa
     4141 aaagaagggg ttaaaaggac ctgctagttt gctgtcgatg aatcagacag aacttgaaga
     4201 aattttagaa ataaatgaat ttattgctta taaaatggca cgaagaattg aaacgatgaa
     4261 tattttttta agtgttctta ataatctgga gggaaatgga attaatgtta ctacaaaata
     4321 tgaagat
//
LOCUS       DAOMTA010000174         4318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_604_length_4318_cov_2.016890, whole genome shotgun sequence.
ACCESSION   DAOMTA010000174 DAOMTA010000000
VERSION     DAOMTA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_604_length_4318_cov_2.016890"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLK68_05995"
     CDS             <1..774
                     /locus_tag="PLK68_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM89997.1"
                     /translation="RKAEVLLINEIKLLIKDLINMPWQDMVGQALDILTRSAGGIMNN
                     ATNFLSTFGVVFTGFIFSLYLLGNKETFLRQLRKAIGALCGYKVTCVIFDYAHKTNEV
                     FSNFISGQLVEACILWVLYYVTMKLFNFPYPELIATIISIFSFVPFFGPIAAMFVGAV
                     LILSKDALMAIWFMVYFQILSQLEDNFIYPRVVGNSVGLPGIWVLLSIFVFGDLFGVF
                     GMVIAVPSAACLYSLASEVINMILKKRKLVITENTIEQK"
     gene            879..1793
                     /locus_tag="PLK68_06000"
     CDS             879..1793
                     /locus_tag="PLK68_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM89998.1"
                     /translation="MEYLKINSISDKLPLDVIVSAPEHPKAIFQIVHGMCEHKERYLD
                     FIEYLNDCGYVVIIHDHRGHGKSVLDETDLGYFYGEGARAIVEDVHQLTNYIKKKYPN
                     LPVCLFGHSMGSLVVRNYIQKYDHEINALIVCGSPSKNKLAGLGKLLCKAIAMVKGDK
                     YHSKLLQKMSFGAFNKGFDKPNEWICSNSQVVDEYNNNPLCTFTFSVNGFYNLLSLMQ
                     NTYKNIDYEANKKLPVLFISGKEDPCLINEKAFNNAVTHLKKQGYQHVISILFEHMRH
                     EILNEKYKETVYSTITTFLVDTMEENDA"
     gene            1786..2730
                     /locus_tag="PLK68_06005"
     CDS             1786..2730
                     /locus_tag="PLK68_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRM89999.1"
                     /translation="MRKRCLLIIDVQTALTSQYPVDENKMIEHIRKLIRVCHNQHLEI
                     VYVRHHDEELILNSDGWQIDSRITPRQDDKIFEKEYNSAFKNTNLDAYLKSQGIQDLI
                     IVGMQSEYCIDTSIKVAFELGYRVIVPKDATTTFDNDIISGKVLKQYLEEKIWKNRFA
                     QVIEVDTLLMMIESKLDFKFSYGKTSFKDAALIRQAVFVEEQGFEKEFDKLDNTAYHL
                     VIYKDEQPIAVGRMYFKDKTTMILGRIAALKEYRGQKLGSKVVTALENKARELGCLET
                     ELSAQQQAQKFYEKLGYKPDGDMYYDEWCPHVTMKKIL"
     gene            2792..3382
                     /locus_tag="PLK68_06010"
     CDS             2792..3382
                     /locus_tag="PLK68_06010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRM90000.1"
                     /translation="MNMKDKTKLFNIPNCLCYFRILLIPVFLFVYFNAQWQHHYIIAA
                     FILVLSGISDCLDGYIARKYNMITDFGKLIDPIADKLTQFTIAITLLFTYPLAWVLLI
                     IIVLKDGMLGIVGLYLYDYGLKIKGASWWGKIATAYFYFVVIILIGYHIPDTFASQLM
                     IITSSALMLLSFVLYAKELKQMIKEKDKLVDEQKRN"
     gene            3363..3743
                     /locus_tag="PLK68_06015"
     CDS             3363..3743
                     /locus_tag="PLK68_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90001.1"
                     /translation="MNKREIKEECYLDMLEDELNSVDAVLNHIEKVEIKEGIFDEHVI
                     QKDLIRAYFDLELALASLCILLRKMSENLFIHIDEDIRRDINSIIHSNKFEYHDHDRI
                     YVYSQKGKEPVDLNNLLRFARSIL"
     gene            3793..3978
                     /locus_tag="PLK68_06020"
     CDS             3793..3978
                     /locus_tag="PLK68_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90002.1"
                     /translation="MDFRKNAGIVLIILGMILTMDRTKDFQGIVSTIAYYIKGYWPLI
                     LCFIGMYILSSPKKKKK"
     gene            complement(4008..>4318)
                     /locus_tag="PLK68_06025"
     CDS             complement(4008..>4318)
                     /locus_tag="PLK68_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRM90003.1"
                     /translation="KDRQQLIKSTKLILDSYYNHDLDTLATIYSYQELPDSEYQQEYQ
                     NYQQLLIIDRNQTMQAKIIEYLNQNKVVFIGVGVGHVVGDLGLITSLSAAGYKVEKLG
                     "
BASE COUNT         1496 a          496 c          788 g         1538 t
ORIGIN      
        1 agaaaagcag aagtattact gatcaatgaa attaaactgc ttattaaaga tttaatcaat
       61 atgccttggc aagatatggt tggtcaagca ctagatatcc taactagaag tgctggggga
      121 atcatgaata atgcaacaaa ttttttatct acatttggag tagtatttac aggatttatt
      181 ttttctctat atttgcttgg aaataaggaa acttttttac gacaattaag aaaagcaatt
      241 ggagcacttt gtggttataa agttacttgt gtaatttttg attatgcaca taaaactaat
      301 gaagtttttt caaattttat cagtggtcaa ttggttgaag cttgtatttt atgggtgttg
      361 tactatgtaa ctatgaagct gtttaatttt ccataccctg aattgattgc tacaattatt
      421 tcaatatttt catttgttcc attttttggt ccaattgcag caatgtttgt aggtgcagtc
      481 ttaattttat caaaagatgc tttgatggca atttggttta tggtatattt tcagatttta
      541 tcacagttag aagataactt catttatccg cgtgttgttg gaaactcagt tggcctacct
      601 ggaatttggg ttttattatc aatttttgtt ttcggtgatt tatttggtgt atttggaatg
      661 gtcattgcgg ttcctagtgc tgcttgttta tattcattag ccagtgaagt aattaatatg
      721 attctaaaga agcggaagct agttattact gaaaatacaa ttgaacaaaa atgattacct
      781 gcataatttg caggtttttg tttatttaga taaaaataag agtttgttta gtttattcaa
      841 gcaatcaata tgatataatc atgaaaagga gaaaaacgat ggagtatcta aaaattaatt
      901 caatatctga taaattacct ttagacgtaa ttgtttcagc gccggaacac ccaaaagcaa
      961 tttttcagat tgttcatggt atgtgtgagc ataaagagcg ttatttagat tttattgaat
     1021 atttaaatga ctgtggatat gttgttatca tacatgatca tcgtggacac ggaaaaagtg
     1081 ttttagatga aactgatttg ggttattttt atggtgaagg tgcccgtgca atcgttgaag
     1141 atgttcacca attaactaat tatattaaga aaaaatatcc aaatttacca gtttgtttat
     1201 ttgggcattc aatgggttct ttagttgtaa gaaattatat tcaaaagtat gatcatgaaa
     1261 ttaatgcatt aatagtttgt ggcagcccaa gtaaaaataa attagccgga ttaggaaagt
     1321 tattatgcaa agcgatagca atggtcaagg gggataagta tcatagtaaa ctattacaga
     1381 aaatgtcttt tggtgctttt aataagggct ttgataagcc aaatgaatgg atttgcagta
     1441 acagccaagt agttgatgaa tataataata atccgctgtg taccttcaca tttagtgtaa
     1501 atggatttta taatttatta agtttaatgc aaaatacata taaaaatata gattatgaag
     1561 caaataaaaa actaccagtc ttatttattt ctggcaaaga agatccttgc ttgattaatg
     1621 aaaaagcatt taataatgct gtaactcatt taaaaaaaca aggctatcaa catgtgattt
     1681 caattttatt tgagcatatg cgccatgaaa ttctcaatga aaaatataag gaaactgtgt
     1741 attcaacgat tacgactttt ttagttgata caatggagga aaatgatgcg taaaagatgt
     1801 ttattaataa ttgatgttca aactgctttg acgagccagt atccagtaga tgaaaataag
     1861 atgatcgagc atattcgaaa actaatcagg gtatgtcata atcaacatct cgaaatcgtc
     1921 tatgttcgtc atcatgatga ggaattgatt ttaaatagtg atggttggca aatagattca
     1981 cggattactc cgagacaaga tgataaaatt tttgaaaagg aatataatag tgcatttaaa
     2041 aatacaaatt tagatgcata tttaaaatca cagggaattc aagatctgat tatcgttggt
     2101 atgcaaagtg aatattgtat tgatactagt attaaagtag catttgaatt aggatataga
     2161 gttattgttc caaaagatgc tacaacgact tttgacaatg atataataag tggaaaagtg
     2221 ttaaagcaat atttagaaga aaagatttgg aagaatcgat ttgctcaagt tatagaagtt
     2281 gatacattat taatgatgat agaatctaag ttggatttta aatttagtta tggtaaaaca
     2341 tcttttaaag atgcagcttt aattcgccaa gctgtatttg ttgaagaaca aggctttgaa
     2401 aaggagtttg ataagcttga caatactgcg tatcatctgg tgatttataa ggatgagcag
     2461 ccgattgcgg ttggacgaat gtattttaaa gataagacaa caatgatctt aggaagaatt
     2521 gcagcgctca aagaatatcg tgggcaaaaa ttaggaagta aggttgtcac tgctttagag
     2581 aacaaagctc gagaattagg ctgtcttgaa actgagttgt cagctcaaca gcaggctcag
     2641 aaattttatg aaaaattagg atataaacct gatggagata tgtattatga tgagtggtgc
     2701 ccgcatgtta caatgaaaaa gatactttaa atcttctatc attgacttat ttaataagtt
     2761 aactataatt atgtaaaaag agggatagaa gatgaatatg aaagataaaa caaagttatt
     2821 taatatacca aattgcttat gttattttag aattttatta ataccagtat ttttatttgt
     2881 gtattttaat gcacaatggc aacatcatta tattattgca gcatttattc ttgtgctttc
     2941 aggtattagt gattgtttag atggttatat tgcaagaaaa tataatatga ttacagattt
     3001 tggaaaatta attgatccga ttgctgataa attaacacaa tttacaattg cgattacttt
     3061 gttatttaca tatcccttag cttgggtgtt attaataata attgtattga aagatggaat
     3121 gttaggaatt gttggattat atttatatga ttatggctta aaaataaaag gagcttcatg
     3181 gtgggggaaa attgcaacag cttattttta ttttgttgta ataattctta ttgggtatca
     3241 cattcctgat acttttgcta gtcagttaat gattatcact agcagtgcat taatgttatt
     3301 gtcatttgtg ttatatgcaa aagaattgaa acaaatgatt aaggaaaagg ataagcttgt
     3361 agatgaacaa aagagaaatt aaagaagaat gttacttgga tatgcttgaa gatgagttaa
     3421 atagtgtcga tgccgttttg aatcatattg aaaaagttga aataaaagaa ggtatctttg
     3481 atgaacatgt tatccaaaaa gatttaatta gagcatattt tgatttagaa ttagcgttag
     3541 caagtttatg cattttactt agaaaaatgt ctgaaaactt gtttattcat attgatgagg
     3601 acatacgtcg ggatattaat agtattattc attctaataa atttgaatat catgatcatg
     3661 atagaattta tgtatattca caaaaaggta aagaacctgt agatttaaat aatttattac
     3721 ggtttgcacg ttctatcctc taatcgacaa attcatttga ttgcccttgc tatgataatg
     3781 ggggtgatta aaatggactt tagaaagaat gcgggaattg tattaattat tttaggaatg
     3841 attttaacca tggatcgaac taaagacttt caaggaatag tatcaacgat tgcatactat
     3901 ataaaaggct actggccttt gattctctgt tttattggaa tgtacatttt aagttcgcca
     3961 aagaaaaaga aaaaatgagg cctagataaa ttctagacct ttatttttta acctaatttt
     4021 tctactttat aaccggctgc gcttaacgat gttattaatc ctaaatcacc aacaacatga
     4081 cctacaccaa cgccgatgaa aacgacttta ttttgattta aatattctat tattttagct
     4141 tgcatagttt ggttacggtc aattattaat aattgttgat aattttgata ttcttgttga
     4201 tattcactat ctggtagttc ttgatagctg tatattgtcg ctaatgtatc aagatcatga
     4261 ttataataac tgtcaaggat caattttgta cttttgatta attgttgtcg atctttta
//
LOCUS       DAOMTA010000175         4303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_607_length_4303_cov_2.459510, whole genome shotgun sequence.
ACCESSION   DAOMTA010000175 DAOMTA010000000
VERSION     DAOMTA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_607_length_4303_cov_2.459510"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1277)
                     /locus_tag="PLK68_06030"
     CDS             complement(240..1277)
                     /locus_tag="PLK68_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HRM90004.1"
                     /translation="MNKEKIRVVQYGCGKMAKYIIRYLYEKGADIVGAIDVNPDVVGK
                     DIGEFAELGFNLGVTISDDADAVLDECDPDIAVVTLFSFMEDCYPHFEKCVRRGINVV
                     TTCEEAIYPWTTSSKLTNKLDELAKETGCTIVGSGMQDIYWINMIGTVAAGCQRIDKI
                     EGAVSYNVEDYGLALAKAHGAGFSPEQFEKELAHPETLEPAYVWNSNEALCNKMGWTI
                     KSQTQKCVPYFYDTDLYSETLGETIPKGNCIGMSAVVTTETFQGPIIETQCIGKVYGP
                     DDGDMCDWKIKGEPDVSFYVQKPATVEHTCATVVNRIPSILLAPPGYMTVEKLDELEY
                     LSYPMHLYCDE"
     gene            complement(1350..2180)
                     /locus_tag="PLK68_06035"
     CDS             complement(1350..2180)
                     /locus_tag="PLK68_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM90005.1"
                     /translation="MKRYTIGKMAKLNNVTEQTLRLYDKMGLFKPAYVDEHNGYRLYD
                     IGQSARLDIIQYLKNLGMTLKEIKEIFDSKNLELLQEKLEESKLQINNQIIQLNEQKA
                     AIQKTIDSFELFKAAPKVGMITLEFINKRTMLYVDEKVNFYDYDLDVYEELLREFKDD
                     LIKQALPPTIFFNPGTILRKEYVLKRKFCATEIFVFIDDKKEIPLKKETIPANMYLCI
                     YCDDFTKEKEYAHRLFDEIESKNYTIVGDYLCESLNDILIFNDKQRNMYLRLQVPIKI
                     "
     gene            2298..4055
                     /locus_tag="PLK68_06040"
     CDS             2298..4055
                     /locus_tag="PLK68_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM90006.1"
                     /translation="MLISANNLSKTNGIKNIVDNVTFSIEETDKVALIGVNGTGKSTL
                     LKVIAGNENYQGDIIRKKDMTISYLPQNPDFDPNNSVIKQVYKLIDASEVNEYEIKAI
                     LNKFGITNYEQLIKELSGGQQKRIALAITLLKPCDLLILDEPTNHLDNKMIEYLEKYL
                     IKFNKAILMVTHDRYFLERVTNKIMEIDRSKIYEYTANYSKFLDLKAQREEADLASQR
                     KRKLFLKKELEWVRAGVQARTTKSKERLQRFEQLNNISDIQTINQVELINTASRLGKK
                     TIELKDLSMCYDDHTLFNNFSYLFKRTDRIGILGINGCGKSTLLKIIAKELEPSSGSV
                     THGDTIKIGYFKQMNDGLDENMRVIDYIKQTSNDLKTLEGSFTAKQMCERFLFDSSLQ
                     HTYIARLSGGEKRRLYLLNILMQAPNVLLFDEPTNDLDISTLAILEDYLDSFNGIVIT
                     VSHDRYFLDRICDGLFVFKNQQITYCNGGYSSYIDISEQQNKNKSDGALKYKEQKKLQ
                     SAIRLSFKEKQELENMEKVILEFEQQIKIINEQMNEYQSNYNKLSELSNQRDYLNEQL
                     EIKNERWLELLEKQEQSQK"
BASE COUNT         1501 a          710 c          605 g         1487 t
ORIGIN      
        1 gctcctaccc ttatctcatc atttgtaatt cgcataattt cattcataat ttcatagccg
       61 tgtcgaggaa cattcaatac tactaaaata tagaacattt gaggcgtgag tgtctcaaac
      121 tcttttcttg ccatttttac cacccttata tcgattatcg atactcttct tacttttatt
      181 atacactaac tatgttgata atcaatatag ttaactaaaa aaagaatccc aaaggattct
      241 tattcatcac aatacaaatg cattggatat gatagatatt ccaattcatc taatttttca
      301 actgtcatat atcctggtgg cgctaataaa atagatggga tacgattaac tacggttgca
      361 catgtatgtt caactgtcgc aggtttttga acatagaaac ttacatcagg ttcaccttta
      421 attttccagt cgcacatatc accatcatca ggtccataga ctttaccaat acattgagtt
      481 tcaatgattg gtccttggaa agtttctgtt gtaactaccg cagacattcc gatacaattt
      541 cctttcggaa ttgtttcacc caatgtttct gaatataaat cagtatcata aaaatatggt
      601 acacattttt gtgtttgcga tttgattgtc catcccattt tattgcacaa cgcttcattt
      661 gaattccata cataagctgg ttctaatgtc tcaggatgag caagttcttt ttcaaattgt
      721 tcaggactaa atccagctcc atgagcttta gcaagtgcta aaccataatc ttcaacatta
      781 taagaaactg caccctcaat tttatcaata cgttgacatc cagcagcaac tgttccaatc
      841 atattgatcc agtatatatc ctgcattcct gaaccaacga ttgtgcaccc tgtttcttta
      901 gctaattcat ctaatttatt agtaagtttt gatgaagtag tccatggata aattgcctct
      961 tcacatgttg ttacaacatt tattccacgg cgaacacatt tttcaaaatg aggatagcaa
     1021 tcttccataa aactaaataa tgtgactact gcaatatctg gatcacattc atctaacaca
     1081 gcatctgcat catcactgat cgttacccct agattaaatc ctaattctgc aaattcacca
     1141 atatccttgc ctacaacatc aggatttaca tcgatagcac caacaatatc agcacctttt
     1201 tcatatagat aacggattat atattttgcc atttttccgc atccatactg tacaactcta
     1261 attttttctt tattcatctt gattacctcc atatactcta agtataaact ttattgttac
     1321 cataaggtca agaatttgtg aaataattat caaattttta ttggaacttg taaccttaaa
     1381 tacatatttc tttgtttatc gttaaatatc aaaatatcat tcaaactttc gcatagataa
     1441 tccccgacta ttgtataatt tttagattca atttcatcaa aaagacgatg tgcatattcc
     1501 ttttctttag taaaatcatc acaataaata catagataca tattagctgg aattgtttct
     1561 ttttttaacg gaatttcttt tttatcatca ataaaaacaa aaatttcagt tgcacaaaat
     1621 tttcttttca aaacatattc cttacgcaaa atagttcctg gattaaaaaa gattgttgga
     1681 ggtaaagctt gtttaatgag atcatcttta aattccctta ataattcttc ataaacatct
     1741 agatcataat cataaaaatt tactttttca tcaacataga gcatcgttct tttatttata
     1801 aattctaatg tgatcattcc aacttttggg gcagctttaa aaagttcaaa tgaatcaatt
     1861 gttttttgaa ttgctgcttt ttgctcattt aattgaataa tttgattgtt aatctgtagc
     1921 ttactttctt ctagtttttc ctgtaataat tctaaatttt ttgaatcaaa aatttctttg
     1981 atttctttta aagtcattcc caaattcttt aaatactgaa ttatgtcaag tcgtgcagac
     2041 tgaccaatat catagagtcg atatccgtta tgttcatcta cataagctgg tttaaataaa
     2101 cccattttgt catataatct tagggtttgt tcagttacgt tatttaattt tgccattttc
     2161 cctattgtat aacgtttcat ttaaatcacc cataaacatt ataccaagaa taaggttttt
     2221 tggtaattga gatgataata tttcgtttac aaatttctat tccatgatag aatagaagaa
     2281 ggtttaagga gacgtatatg ttaataagtg caaataattt atcaaaaacg aatggtatta
     2341 aaaatattgt tgataatgtt actttttcaa ttgaagaaac cgataaagtt gctttgattg
     2401 gagttaatgg tacggggaaa agtaccttgt tgaaagtcat tgctggaaat gaaaattatc
     2461 agggagatat tattagaaaa aaagatatga caatctcata cttaccacaa aaccctgatt
     2521 ttgatcctaa taacagcgtt attaaacaag tttataaatt aattgatgcc agcgaagtta
     2581 atgaatatga aatcaaagca attttaaata agtttggaat tactaattat gaacagttaa
     2641 ttaaagaatt atcaggtggt cagcaaaagc gtattgccct tgcaatcacc ttgctaaaac
     2701 catgtgatct cttaattctc gatgaaccaa ctaatcatct agataacaaa atgattgaat
     2761 atttagaaaa atatttaatt aagttcaaca aagccattct aatggttacc catgatcgtt
     2821 attttcttga acgagttacc aataagatca tggaaattga tcgtagcaaa atttatgaat
     2881 acactgctaa ctattcaaaa ttccttgatt taaaagcaca acgcgaagaa gctgatctag
     2941 cttcccaaag aaaaagaaaa ttattcttaa aaaaagaatt agagtgggtt agagctggag
     3001 ttcaggctag aactactaaa agcaaagaga gacttcaacg atttgaacag ctcaacaata
     3061 tttctgatat tcaaactatt aatcaagtag aattaatcaa tactgcttct agacttggta
     3121 aaaagacaat tgagttgaaa gatttgagta tgtgctacga tgatcatacc ttgtttaaca
     3181 acttttctta cttatttaaa cgaacagatc gaattggaat cctcggaata aatggttgtg
     3241 gtaaaagcac ccttctaaag attatcgcta aagagttaga accaagcagt ggatcagtta
     3301 ctcatggaga tacaattaaa atcggatatt ttaaacagat gaatgatggt cttgatgaaa
     3361 atatgcgcgt gattgactat atcaaacaaa caagtaatga tttaaaaacc cttgaaggca
     3421 gctttactgc taaacaaatg tgtgaacgtt tcctttttga ttcaagttta caacatactt
     3481 atattgctcg tttatcaggt ggagaaaaac gtcgtctata tttgttgaat attttgatgc
     3541 aagctcccaa tgtactattg tttgatgagc caactaacga tttagatatt tccactttag
     3601 ctattcttga agattatctt gatagtttta atggcatagt cattaccgta tctcatgatc
     3661 gttattttct tgatcgtatt tgtgatggct tattcgtttt taagaatcaa caaatcactt
     3721 attgtaatgg tggctatagc agttatatcg atatttctga acaacaaaat aagaataaaa
     3781 gcgacggagc tttaaaatat aaagaacaga aaaaacttca aagtgctatt cgcttaagtt
     3841 ttaaagaaaa acaagaactt gaaaatatgg aaaaggttat tcttgagttt gaacaacaaa
     3901 tcaaaattat taatgaacaa atgaatgagt atcaaagtaa ttataataaa ttgtcagaat
     3961 tatcaaatca gcgtgactat ttaaatgaac aacttgagat taaaaatgaa cgctggttag
     4021 agctattaga aaaacaagaa caaagtcaaa aataaaatag cgccttatgc gctattttat
     4081 ttatattgat ttaaaaaaat tatgataaca tcaaagaccg ctctaacata aaaaattgaa
     4141 cgcccatgat cagctttagc aactttgcat aaagttgcat tatgaccata ctctttttat
     4201 ttttgatata attctactga ttgttcaaaa ggaacaacat gctccttaga accatgcaga
     4261 ataagaattg gtagcaaatt cattttcgct tcaagataag ata
//
LOCUS       DAOMTA010000176         4302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_608_length_4302_cov_2.139157, whole genome shotgun sequence.
ACCESSION   DAOMTA010000176 DAOMTA010000000
VERSION     DAOMTA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_4302_cov_2.139157"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLK68_06045"
     CDS             complement(<1..625)
                     /locus_tag="PLK68_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM90007.1"
                     /translation="MNYKDTLLMPKTEFEMRGNLPKKEPKYVERWQKENMYERVIEQN
                     KGKEDFVFHDGPPYANGNMHMGHMLNKVIKDVICRYNNMEGRYTPYIPGWDTHGLPIE
                     NAIQKLGVNRKEMSTAAFREKCDEYAHEQVNKQMEQLIRMGTFADYKHPYLTLMPEFE
                     ANQIDVFAKMAMDGLIFKGLKPVYWSPSSESALAEAEIEYHDIKSPTI"
     misc_binding    complement(666..868)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(865..1296)
                     /locus_tag="PLK68_06050"
     CDS             complement(865..1296)
                     /locus_tag="PLK68_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRM90008.1"
                     /translation="MSEFKKQFRGYSVQQVDSKIDDYQAELASLKQQVASLTDELDHV
                     KEQNSLLQHRVNITEKTNEEIARLALKEASELIDKAKRNANMILKESLDYVRSLSSEM
                     NDFKDQAIKFRSSVQKMSQDILDSIDNSEVFNLINEEDEDN"
     gene            complement(1309..2067)
                     /locus_tag="PLK68_06055"
     CDS             complement(1309..2067)
                     /locus_tag="PLK68_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HRM90009.1"
                     /translation="MIEHFKGEEVFVKKVLEFKDQALYKQRLVLTKFLNPYHQSIVYS
                     IVGNQNDLIVIEDGGMVDSEMKRLIIAPSFYQIEKEDFEIVLAKISYAKPFGTLNHRD
                     ILGALMSLGVKRELFGDIYEYEDNFYVAMDAKIYEYVKNNLIKIKRSKVKILESEEII
                     TIKHQYISKTFIVSSFRLDKVVSTLYGVPRSKAVCYIQSGFVKVNHKEVEEINYLCNN
                     SDIISLRRHGRVKFVDTKRRTKQDNYVVEGYFYK"
     gene            complement(2103..2534)
                     /gene="sepF"
                     /locus_tag="PLK68_06060"
     CDS             complement(2103..2534)
                     /gene="sepF"
                     /locus_tag="PLK68_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRM90010.1"
                     /translation="MGYGQKVKKWFFDEDGDEVEEYEDVEIDTEPKTSLFEQAKFSKT
                     SDAIKALNANKDSQLILFEPRAFAETQDIANHLKQKKAAVVNLHRLQKEQSKRVVDFL
                     SGVIFAIEGDIQRIGPRIFLCTPKNIGVSGTIDLDEDESEE"
     gene            complement(2669..2878)
                     /locus_tag="PLK68_06065"
     CDS             complement(2669..2878)
                     /locus_tag="PLK68_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HRM90011.1"
                     /translation="MINVVDGCKIGFISDIEIDWCGKCIQAIVVEKYSFFKLLCFFKE
                     APCIVIPIECVVSIGGDVILVSIEP"
     assembly_gap    2881..2890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2923..3699)
                     /locus_tag="PLK68_06070"
     CDS             complement(2923..3699)
                     /locus_tag="PLK68_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HRM90012.1"
                     /translation="MSNYQVIINGYQEPKKRLTTDETYKLIDEYQATKNEAIKDRLVN
                     DNTKLVLSMARRFYGREDSMDDLFQVGMIGLIKAIENFNTSFGLKFSTYAVPLIIGEM
                     KRYLRDNHQIKISRSIRDLAYKVLKVKDSYITKLNREPTINELANELEVEPSAVIEAL
                     LSTNSVSSLQEEVKNDDGNNLKMIDSITDDKTVVSRTNETIDLYDALKSLNQKEHRVI
                     KQRYFEGLSQSEIAKELFISQAQVSRIERKALDNLHNYLK"
     gene            complement(3748..>4302)
                     /locus_tag="PLK68_06075"
     CDS             complement(3748..>4302)
                     /locus_tag="PLK68_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM90013.1"
                     /translation="RDLLIEHNLRLVVYVAKRYDTTQNGGIEDLISIGTIGLVKAINT
                     FKPDKNIKLATYASRCIENEILMFLRKNNKLRHEISLDEPLNIDYDGNELLLSDIIGT
                     DSDIVKNELEQSDQKAMFYEAFKDLSKREKEILMFRYGLMNYDELTQKDVAKMMGISQ
                     SYISRLEKKIIKKLRNKLNYNEIK"
BASE COUNT         1362 a          836 c          481 g         1613 t
ORIGIN      
        1 aaatagtcgg actcttaata tcatgatatt caatctcagc ctctgctaaa gctgattcac
       61 ttgatggtga ccaatagaca ggttttaacc ctttaaaaat taatccatcc attgccattt
      121 ttgcaaatac atcaatttga ttagcttcaa actcgggcat taaagtcaaa tatggatgtt
      181 tataatcagc aaatgtcccc attctgatta attgttccat ttgtttattt acttgctcat
      241 gagcatattc atcacatttt tctctaaaag ccgctgtaga catttcttta cgatttaccc
      301 ctaatttttg aatcgcattt tcaattggta atccatgcgt atcccaacca ggaatatatg
      361 gcgtatagcg tccctccata ttattgtaac gacagataac atctttgatt accttattta
      421 acatatgtcc catatgcata ttaccattgg catatggtgg accatcatga aaaacaaaat
      481 cttctttccc tttgttttgc tcaattactc tttcgtacat gttctctttt tgccagcgtt
      541 caacatactt aggttctttt ttaggaagat tcccacgcat ttcgaactca gtttttggca
      601 ttaatagtgt gtccttatag ttcatttctt ttcctccaaa taaattaaaa aacgtccttt
      661 caaaataaaa ggacgaataa ttcgcggtac caccttaatt cgtattttcc aatacgcact
      721 caaacatctt aacgcgatta acggcaaccc ctactaattt caaggtgcta ctccagagtg
      781 atccattata ataatagtta ctgattttca ccatccatca gctctctaca aacatcttac
      841 tataaactgt ctctttcatc gtatttagtt atcttcatct tcttcgttaa taagattaaa
      901 gacttccgaa ttatcgattg aatccaaaat atcttgggac attttttgaa ccgatgaacg
      961 gaatttaatt gcctgatctt taaaatcatt catctcacta gataaactac gaacatagtc
     1021 caatgattct tttaagatca tattggcatt gcgtttagcc ttatcaatca attcacttgc
     1081 ttcttttaag gctagacgag caatttcttc attagttttt tcggtaatat tgacacgatg
     1141 ctgcaatagc gagttttgtt ctttaacatg atctaattca tctgttaaac tagcaacttg
     1201 ctgctttagt gatgctaact cagcttgata atcatctatt ttactatcaa cttgttgaac
     1261 actatagccc ctaaattgct ttttaaattc actcatcgtc tcactcccct acttataaaa
     1321 atacccttca acgacataat tatcctgttt agttcgtcgt ttagtatcga caaatttaac
     1381 tcttccatga cgtcttaacg atattatatc actattatta cacaaataat ttatttcttc
     1441 aacttcttta tgattaactt ttacaaaacc tgattgaata taacagacag ccttgcttcg
     1501 aggcactcca tataaagttg aaacaacttt atctaaacga aatgaggaga caataaaagt
     1561 tttacttata tattgatgtt taattgtaat aatctcttca ctctctaaaa ttttaacttt
     1621 actacgttta attttaatta agttattttt tacatactca tagatttttg catccatggc
     1681 aacataaaaa ttatcctcat actcataaat atccccaaat aattcgcgtt taacccctaa
     1741 agacattaat gctcctaaaa tatctcgatg attcaatgta ccaaatggtt ttgcataact
     1801 tattttagct aaaactattt caaaatcttc tttttcaatt tgataaaatg atggtgcaat
     1861 aatcaaacgt ttcatttcac tatcaaccat tccaccatct tcaataacaa tcaaatcatt
     1921 ttgattccct acaatgctat aaacaattga ttgatgatat ggatttaaaa atttagttaa
     1981 aacaagtctt tgtttatata gagcttgatc tttaaactct aatacctttt taacaaaaac
     2041 ttcttcacct ttaaaatgct ctatcataat taaaaagtat tagctaagct aatacttatc
     2101 tattattctt cagattcatc ttcatctaaa tcaattgtcc cagaaacacc aatgttttta
     2161 ggagtacata agaatattct aggtccaatt ctttggatgt ctccttcaat tgcaaagata
     2221 actccgctta aaaaatctac aactcgttta gattgttctt tttgtaaacg atgcaagtta
     2281 acaacagcag ctttcttttg ctttaaatga tttgcaatat cttgagtttc tgcaaatgcc
     2341 cgtggttcaa ataaaattaa ttggctatct ttatttgcat ttaaagcttt gattgcatca
     2401 cttgttttag aaaactttgc ttgctcaaat aagcttgttt taggttctgt atcaatctct
     2461 acatcttcat attcttcaac ttcgtcaccg tcttcgtcaa aaaaccattt ttttactttt
     2521 tgtccgtatc ccatgaagtg gtcctcctta atttattact agtatattat agcacaataa
     2581 atccgtccat aaaaggataa ttactcaaca aactgctggt tatttaaaag attttattaa
     2641 aatagtcaaa tgcttaaaca tttgactatt atggctcaat cgatacaaga atcacatcgc
     2701 caccaatgct cactacacat tcaattggaa taacaataca tggtgcttct ttaaaaaaac
     2761 ataatagttt aaaaaagcta tatttctcta ctacaattgc ctgtatacat ttaccgcacc
     2821 agtcaatttc aatatcgctg ataaaaccaa ttttgcagcc atcaacgaca ttgatcacgt
     2881 nnnnnnnnnn aaatctcatc gaatcaccat taatatatat gcttatttta gataattatg
     2941 taaattatct aaagcttttc tttcgattct tgacacttga gcttgggaaa taaataattc
     3001 ctttgcaatc tcactttggg acaacccctc aaaatatctt tgtttaatca cacgatgctc
     3061 tttttgattt aatgatttta aagcatcata taaatctatt gtttcatttg ttcttgatac
     3121 aactgtttta tcatctgtaa tcgaatcaat catctttaaa ttattaccat catcattttt
     3181 aacttcttct tgtaatgaag aaacactgtt tgttgataac agcgcttcaa taacagcact
     3241 tggctcaact tccagttcat tagctaattc atttatcgtt ggttcacgat ttaattttgt
     3301 tatatagcta tctttaacct ttaaaacctt atacgctaaa tctcgaattg agcgagaaat
     3361 cttgatttga tggttatcac gaagataacg tttcatctca ccaattatta gaggaactgc
     3421 atatgttgaa aatttcaaac caaaactagt gttaaaattt tcaattgctt taatcagccc
     3481 aatcattcca acttgaaata agtcatccat gctatcttca cgaccataaa aacgtcttgc
     3541 cattgataaa actaacttag tattatcgtt aactaatctg tcttttatcg cctcgttttt
     3601 agtagcttgg tattcgtcta tcaatttata agtttcatcg gttgtaagtc tttttttcgg
     3661 ttcttgataa ccatttataa taacttgata attactcata tttgccttct ttcaactcta
     3721 tttttagcaa atatgacaaa tttatactta ttttatttca ttataattta atttattacg
     3781 taattttttg attattttct tttctaatcg tgaaatatat gactgtgaaa ttcccatcat
     3841 tttagcaaca tccttttgcg tcaactcatc ataattcatt aaaccataac gaaacatcaa
     3901 aatttccttt tcacgtttgg ataaatcctt aaaagcctca taaaacattg ctttttgatc
     3961 actttgttcc aattcatttt taacaatatc actatctgtt ccaataatat ccgataacaa
     4021 caattcattt ccatcataat caatatttaa tggttcatct aaagatatct cgtgacgcaa
     4081 tttattattt tttcttagaa acatcaaaat ttcattttct atacaccgtg atgcataagt
     4141 agctaactta atatttttat caggtttaaa agtattgatt gctttaacta aaccaatcgt
     4201 tccgatacta atcaaatctt ctattccacc attttgcgtc gtatcatagc gttttgcaac
     4261 atatacaact agccttaaat tatgctcaat caacaaatct cg
//
LOCUS       DAOMTA010000177         4301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_610_length_4301_cov_2.661799, whole genome shotgun sequence.
ACCESSION   DAOMTA010000177 DAOMTA010000000
VERSION     DAOMTA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_610_length_4301_cov_2.661799"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4286)
                     /locus_tag="PLK68_06080"
     CDS             complement(<1..4286)
                     /locus_tag="PLK68_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM90014.1"
                     /translation="MNPSSSNWWENQIGVQLRFSRIALFMESIVSKDAMDIMLNKLLE
                     KTPVKYGTGQNNLWFDQNYVYYAIITENGTKHTNSTGFKKLDLKELVDDYLSYCLVVQ
                     TDDNTAEAVQVDNSFYMHGRQFYSNGYGMSMFRDMSFWIYMLRETSYAFEQDVIDLMA
                     DYMIDGTSWTIRGDIMELYLGYRPYDYDVGYDNYAAEYIDPLKRMIESDPDRADEYQA
                     ILDNIQGKNTVNGKNGNYYMWRSGYASHMRDGYGVNIKMDSNQIIGGEWRGSWSGYDK
                     DGGRLIYWTSSAASTITVDGDEYTNVYPTYDWAHCPGTTTAARIVQDYANAGRFTNGT
                     EHTIGVSNGKYGNTAYDMNKKGTQVKKGYFFFDDEFVALGSGIHTTLNQCEAEDVNVG
                     GQSVAEGTKEQIYNTNWLYNGKVGYVFLENTDVVVSNSVQTNNPSLWDEAKKNETPAT
                     FTAYLDHGLKPSNDSYAYIVVPNTTAGAVSQYAGNTPVTVIANNEKVQAVRHDGLKQT
                     QINFYQAGSLEYKDGYIITVDQPCSIIIDESEDTRKISVAVNDTAANQTVNVNLNYDN
                     QETQTTFVSGALPYAGQTMTLSEGSDNRYHTNSSMIGHEVEKAFDGDENTYWQSEETQ
                     NDWISMFTGNNKHLASMNILWGDNYASAYDVMVSQDGKNYELLKSVSNGDGKTDTIEL
                     KGVYPYIKIIMKDGNGNCFEIKEITFKASDSIALNKSVEASSTSTNDPGNTKELVVDG
                     NTSTRWSSLRNEDENWIMVDLGQYSEINAMSIQWESACSDDYDIQVSSDKNNWITVKD
                     SMATGSSLLDEYNFDEPVYGRYVRVSSHKSRTLKYGISIYEISVYGSYKDEDIAANKN
                     AFSSTIKDLNLPTHAIDNKANTSWISSAAGDQWIYVELKGIYKISSMQVDWGNNYAPN
                     YEIQISDDAQMWQTVKTIIDGQGGNENISDLGNQEARYVRLKLNGSSGSSYQIIQWSV
                     YGELVEAENLENIALNKTATASSVYNNVYTANRAIDGSFENNGGNDQSRWVSNRNSND
                     EYIQIDLEANYDLTGVKLYWEGNGAKKYQILVSEDGKTWQKVYLEENGQPGIANIPFN
                     ETVTARYVKMLGIECASKYGYSLWEFEVYGKLHQEPVVPEVNIALNKTSKASSEFTDP
                     NDGNKTYQSLLAFDGKGTNETVDGKQSRWVSLRTKDDPTATSQWIYVDLEADYDISKV
                     VLNWEGNGAKEYKVQISNDGQNWTDISHITDGKGGIDELTYKNTTGRYVRMLGIEPGG
                     IYGYSLWEFEVYGEAVLEPENPNVNLALNKSSNASSEYVDSKDGNKTYESSLAFDGKG
                     TNEIVDGKQSRWVSNRKSNDEWIYVDLQDIYNISKVILNWEGSGAKKYKVQVSNDGQV
                     WTDISDINDGDGGLDELSYKDVTGRYVRMLGIEVGSDYGYSLWEFEVYGTT"
BASE COUNT         1298 a          904 c          530 g         1569 t
ORIGIN      
        1 gttgttccat aaacttcaaa ttcccatagt gaatacccat aatcacttcc aacttcaatc
       61 cctaacatcc ttacatatcg tcctgttaca tctttatatg ataattcatc aagacctcca
      121 tcgccatcat taatatctga tatatctgtc caaacttgac catcattaga aacttgcact
      181 ttgtatttct ttgcaccgct tccttcccaa ttcaaaataa ctttagaaat attataaata
      241 tcttgtaagt ctacatatat ccactcatca ttagatttcc gattagatac ccagcgtgat
      301 tgctttccat caactatttc atttgtacct ttaccatcaa acgctagcga agattcatat
      361 gttttatttc catctttcga atcaacatat tctgagctcg cattcgaaga tttattcaaa
      421 gctaaattta catttggatt ttcaggttct aatacagctt ccccgtaaac ttcaaattcc
      481 cataatgaat atccataaat tcctccaggc tcgattccta acattcttac gtatctgcct
      541 gttgtatttt tatatgtcag ttcatcaata ccacctttac catctgtaat atgtgatata
      601 tctgtccaat tctgaccatc attagaaatt tgcactttat attctttagc accatttcct
      661 tcccagttaa gaacaacttt agaaatatcg taatcagctt ctaagtcaac atagatccat
      721 tgtgatgttg ctgttggatc atcttttgtt cttaaagata cccagcgtga ttgctttcca
      781 tcaactgttt catttgtacc tttgccatca aacgctaata aagattgata tgttttatta
      841 ccatcattag ggtcagtaaa ctcgctactt gccttagagg ttttattcaa tgcaatattt
      901 acttcaggca ccactggttc ttggtgtaat tttccataca cttcaaattc ccataatgaa
      961 tatccgtatt tagaagcaca ttctatacct agcattttaa cataccgagc agtaacagtt
     1021 tcattaaatg ggatatttgc aattccaggt tgtccattct cttctagata aaccttttgc
     1081 catgtttttc catcttcaga gacaagaatt tgatattttt tagcaccatt tccttcccaa
     1141 taaagtttta cacccgttaa atcataattc gcttctaaat ctatttgaat atattcatca
     1201 ttcgaatttc tatttgaaac ccatcttgat tgatcatttc caccattatt ttcaaaactt
     1261 ccatcaatag ctctatttgc agtataaaca ttgttataaa ctgaagaagc tgttgctgtt
     1321 ttatttaaag caatattctc taaattttct gcttcaacaa gttcgccata aactgaccac
     1381 tggatgattt ggtatgatga gccactagaa ccatttaatt ttaatctaac atatcgtgct
     1441 tcttgattac ctaaatctga aatgttctca ttaccacctt gtccatcaat tatagttttt
     1501 actgtttgcc acatttgtgc atcatcagaa atctgaattt cataattagg tgcataatta
     1561 tttccccaat caacctgcat acttgaaatt ttataaattc cttttaattc tacatagatc
     1621 cattgatcac cggctgcact cgaaatccaa gacgtattgg ctttattatc aattgcatga
     1681 gtcggtaagt tcaaatcctt tattgtactt gaaaatgcat ttttattagc tgcaatatcc
     1741 tcatctttat aagaaccata cacagaaatt tcataaatac taataccata ttttaaagtt
     1801 cttgatttat gtgacgaaac tctaacataa cgcccatata caggttcatc aaaattgtat
     1861 tcatccaata agctacttcc agttgccata ctatctttaa ccgttatcca gttatttttg
     1921 tcactagaga cctgaatatc ataatcatca gaacaagcgg attcccattg aatgctcata
     1981 gcattaattt ctgaatattg tcccaaatca accatgatcc agttctcatc ttcatttcgt
     2041 aatgaactcc atcttgtact tgtattacca tctacaacta attctttagt atttccaggg
     2101 tcatttgttg acgtactgct tgcttcaaca gatttattaa gcgctattga atcacttgct
     2161 ttaaatgtaa tttccttaat ttcaaaacaa tttccatttc catctttcat gataatctta
     2221 atatatgggt agactccttt taattcaatc gtgtctgtct taccatcacc attacttaca
     2281 gatttcaata attcataatt tttaccatct tgagaaacca taacatcata agcactagca
     2341 taattatcac cccaaagaat attcatagat gctaaatgtt tattgttgcc agtaaacata
     2401 ctgatccaat cattttgcgt ttcttcactt tgccaatatg tattttcatc accatcaaaa
     2461 gctttttcga cttcgtgtcc aatcattgaa gaattagtat gataacgatt atctgagcct
     2521 tctgataacg tcattgtttg tccagcatat ggtaatgctc ctgaaacaaa tgttgtttgt
     2581 gtttcttgat tatcataatt taaatttaca tttacagttt gattggctgc tgtatcattg
     2641 actgctacag agatttttct tgtatcctct gattcatcaa taataatact gcaaggttga
     2701 tcaacagtaa ttatatagcc atctttatat tccaatgaac cagcttgata gaaattaatt
     2761 tgagtttgtt ttaaaccgtc atgtcttacc gcttgaactt tttcgttatt agcaattaca
     2821 gtaactggtg tattaccggc atattgacta actgcacctg ctgtggtatt tggtacgaca
     2881 atataggcat agctatcatt gcttggcttt aatccatgat ctaaataggc tgtaaaagta
     2941 gccggagttt cattcttttt tgcttcatcc cataatgacg gattattagt ttgaacacta
     3001 ttacttacaa ctacatccgt attttctaag aaaacatatc caacttttcc attatatagc
     3061 caattagtgt tataaatttg ttctttcgtt ccctcagcca cactttgacc accaacattt
     3121 acatcttcag cttcgcattg atttaatgtt gtatggatcc ccgaacctag agcaacaaat
     3181 tcatcatcaa agaagaaata tcctttcttt acttgagtcc cctttttatt catatcataa
     3241 gcagtatttc catatttacc attggatacc ccaattgtat gttcagttcc atttgtaaaa
     3301 cgacctgcat tagcataatc ttgaacgatt cgtgctgctg ttgttgttcc tggacaatga
     3361 gcccaatcat atgttggata aacgttagta tattcatctc cgtcaactgt gattgttgaa
     3421 gctgctgatg atgtccaata aattaatcgt cctccgtctt tatcataacc tgaccagctg
     3481 ccacgccatt caccaccgat gatttggtta gagtccattt taatattaac tccgtaccca
     3541 tctcgcatgt gagaagcata accagaacgc cacatataat aattaccatt ttttccatta
     3601 actgtatttt ttccttggat gttatcaagg attgcttgat attcatcagc tcgatcagga
     3661 tctgattcaa tcattctctt aagtggatcg atatactctg ctgcatagtt atcatagcca
     3721 acatcataat catatggccg atatccaagg taaagctcca taatatctcc tctaattgtc
     3781 cagcttgtcc catcaatcat ataatcagcc attaaatcaa tgacatcttg ttcaaatgca
     3841 taactagttt ctcttagcat ataaatccaa aaactcatat ctctaaacat tgacattcca
     3901 tatccattag aatagaactg cctaccatgc atatagaaac tgttatcaac ctgaactgct
     3961 tcagcagtat tatcatcagt ttgaacaact aagcaataac ttaaataatc atctacaagt
     4021 tcttttaaat ctaatttctt aaatcctgta gaattggtat gtttagtccc attttctgta
     4081 ataattgcgt aataaacata attttgatca aaccataaat tattttgacc tgtaccatat
     4141 ttaactggtg ttttttccaa taatttatta agcataatat ccattgcatc tttacttaca
     4201 atgctttcca taaataaagc aattctagaa aatcttaatt gaactccaat ttgattctcc
     4261 caccagttag aagaagatgg attcattttt ccccaataaa c
//
LOCUS       DAOMTA010000178         4297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_613_length_4297_cov_2.341584, whole genome shotgun sequence.
ACCESSION   DAOMTA010000178 DAOMTA010000000
VERSION     DAOMTA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_4297_cov_2.341584"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PLK68_06085"
     CDS             complement(<1..127)
                     /locus_tag="PLK68_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016132269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRM90015.1"
                     /translation="MKRALVSVTNKDGIVDFCKGLVDLGFEIVSTGGTMAKLQAEN"
     gene            complement(129..722)
                     /gene="purN"
                     /locus_tag="PLK68_06090"
     CDS             complement(129..722)
                     /gene="purN"
                     /locus_tag="PLK68_06090"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRM90016.1"
                     /translation="MLKIAVFVSGGGTDLQSVIDAVKNNSINGEIAIVISNRKNAYGL
                     ERARQAGIETAVVRKDDELIVKMLKERNVGLVVLAGYLAILTDVLIDAYPNKIINIHP
                     SLIPSFCGPGHYGMHVHEKVLARGVKVTGATVHFVSSEVDGGPIILQEACNIDDLDNA
                     EDIQARVLEIEHRILPKAVALFCDGKIIVENERAKVI"
     gene            complement(716..1741)
                     /gene="purM"
                     /locus_tag="PLK68_06095"
     CDS             complement(716..1741)
                     /gene="purM"
                     /locus_tag="PLK68_06095"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRM90017.1"
                     /translation="MDYKKAGVDIEAGYKSVELMKKHVKETMRPEVLGGLGGFAGAFD
                     LSAIKDMDEPVLLSGTDGCGTKVKLAFVMDKHDTIGIDAVAMCVNDIACSGGEPLFFL
                     DYIACGKNYPEKIATIVSGVAEGCKQSGCALVGGETAEHPGLMPEDDYDLAGFAVGVV
                     DKKDIINGENIKPGDVLVGIASSGVHSNGFSLVRNVFEMNKETLDTYYDELGKTLGEA
                     LIAPTRIYVKALKNVKDAGVRIKGCSHITGGGFDENIPRMLPDGVKAVIKKDSYKVPA
                     IFDLIAKKGDIAEEMMYNTFNMGLGMVIALDPADIETSMAAIKAAGDECYIIGSIEAG
                     DKGVQLC"
     gene            complement(1758..2441)
                     /locus_tag="PLK68_06100"
     CDS             complement(1758..2441)
                     /locus_tag="PLK68_06100"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRM90018.1"
                     /translation="MAELLYEGKAKQVYKTDKEDEYLIHYKDDATAGNGVKHDQFEGK
                     GVLNNTISCIIFDMLEEAGIKTHMIKKLNERDILVKKVDIFPLEVIIRNITTGSFCKR
                     LGFPEPIFELSYKNDDYGDPLINEDHAVALKLCTREEYNFIKQETLKINELLKEFFLS
                     LNLKLVDFKIEFGKTPDGQILLADEISPDSCRLWDVETNQKYDKDVFRQDIGDLIETY
                     KAVLARMQK"
     gene            complement(2463..2951)
                     /gene="purE"
                     /locus_tag="PLK68_06105"
     CDS             complement(2463..2951)
                     /gene="purE"
                     /locus_tag="PLK68_06105"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRM90019.1"
                     /translation="MKVAIIMGSTSDLSKVEPAIGILKDYGVEVNVRCLSAHRAHLGL
                     STFIKETETDGTEVIITAAGMAAALPGVVASQTVLPVIGVPISGATLDGMDALLSIVQ
                     MPSGIPVATVAINGSKNAAYLALQIMAIKHDKIKEKLLAYRKDMEKQAMSANEEVIAK
                     YK"
     gene            complement(3000..>4297)
                     /locus_tag="PLK68_06110"
     CDS             complement(3000..>4297)
                     /locus_tag="PLK68_06110"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRM90020.1"
                     /translation="FTGDIHAITAANNLIAAMLDNSIHQGNPLNIDVRNIVWRRVVDL
                     NDRALRNTICGLGGKVNGMPREDGFDITVASEVMAILCLATSLEDLKERAGKMIVAYD
                     YAGNPVTVNDIEATGAVTLLLKDAIKPNLVQTLDHTPVFVHGGPFANIAHGCNSVMAT
                     KLAIKLGDYAITEAGFGADLGAEKFLDIKCRQANLDPQAVVIVATVRALKMHGGVDKK
                     ELGTENLEALAKGIKNLEKHIENIAKYNLPSVVAINAFPTDSEAELQLLKDTCNKMGV
                     DVAISKVWEKGADGGIELAEKLLEILDTKEANYQPLYDLNLSIKEKIETIAKEIYGAD
                     GVAFDKKVLTKMKKYEAQGLADLPICVAKTQYSLSDQPTLLGRPRGFTIKINDLIPSA
                     GAGFLVAISGSIMRMPGLPKRPAAVNMDIDKDGKIVGLF"
BASE COUNT         1299 a          941 c          541 g         1516 t
ORIGIN      
        1 cattttctgc ttgtaattta gccatcgttc ccccagtgga aacaatttca aaacctaaat
       61 ctactaggcc tttacaaaaa tcaacgatac catctttatt agtaacacta actaatgctc
      121 ttttcatttt aaatcacctt agccctttca ttttcaacaa taattttacc atcacaaaat
      181 aaagcaactg ctttaggtaa aatgcgatgt tcaatttcta atacccgtgc ttggatatct
      241 tcagcattat ctaaatcatc aatattgcac gcctcttgaa gaataatcgg tcctccgtca
      301 acctcactac taacaaaatg aactgttgca ccagtcactt ttacccctcg cgctaaaact
      361 ttttcatgaa catgcattcc ataatgccct ggtccacaaa atgatggaat caatgaagga
      421 tgaatattaa taattttatt aggataagca tcaatcaata catccgtaag gatcgctaaa
      481 tatcccgcta aaacaactag accaacattg cgttctttta acattttaac aattaattca
      541 tcatctttac gcactacagc agtttcaatt cctgcctgtc ttgcacgttc aagtccatat
      601 gcattcttac gattagaaat aacgattgca atttcaccat tgatgctgtt atttttaaca
      661 gcatcaatga ccgattgcaa gtctgttcct cccccagaaa caaagacagc aatttttagc
      721 ataattgcac tcctttgtca cctgcttcaa tacttccaat aatataacat tcatcacctg
      781 ctgctttaat tgccgccata ctagtttcaa tatcagctgg atctaacgca atgaccatac
      841 ctaatcccat gttaaatgtg ttatacatca tttcctctgc aatatcacct tttttagcaa
      901 tcagatcaaa aattgcgggt actttatagc tatctttttt aataactgct tttactccat
      961 cgggtaacat tcttgggata ttttcatcaa aaccaccacc agttatatga gagcagcctt
     1021 ttattcttac cccagcatct ttaacatttt ttaatgcttt tacataaatt ctagttggtg
     1081 caattagggc ttcgcctaat gtttttccaa gttcatcata atatgtatct aaggtttctt
     1141 tattcatctc aaatacgttt cgaactaatg agaatccatt actatgaact ccacttgaag
     1201 caattcccac taaaacatca cctggtttaa tgttttcacc attaataata tcttttttat
     1261 caaccacccc gactgcaaaa ccagctagat catagtcgtc ttcaggcatt aatccaggat
     1321 gttccgctgt ttcaccacca actaaagcac aaccagattg tttacagcct tcagcaaccc
     1381 cacttacgat cgtcgcaatt ttttccggat agttttttcc acacgcaata taatcaagga
     1441 agaacaatgg ttctccacct gaacaggcaa tatcattaac acacattgca actgcatcaa
     1501 taccaattgt atcatgttta tccattacaa aagcaagttt tacttttgtt ccgcatccat
     1561 cagttcctga taaaagaaca ggttcatcca tatctttgat tgcacttaaa tcaaatgctc
     1621 ctgcaaaacc accaagacca cctaaaacct ccggacgcat tgtttcctta acgtgttttt
     1681 tcattaattc aactgatttg tatcctgctt caatatctac tccagccttt ttgtaatcca
     1741 ttttttaatc tcctttatta tttttgcatt cttgctaata ctgctttata agtttcgatt
     1801 aaatcaccaa tatcttgtct aaatacatct ttatcgtatt tttgattagt ttcaacatcc
     1861 cataaacgac aagaatctgg agaaatttca tcagctaaca agatttgacc atcaggtgtt
     1921 ttcccaaatt caattttaaa atcaactaat tttaaattta agcttaagaa aaattccttt
     1981 aataattcat ttatttttaa tgtttcttgt ttaataaaat tatattcttc tcttgtacat
     2041 agttttaagg caactgcgtg atcttcatta atcaatggat caccataatc atcattttta
     2101 taacttaatt caaaaattgg ttcaggaaat cctaatcttt tacaaaaaga ccctgttgta
     2161 atatttctaa taattacttc taatgggaag atatcaactt tttttactaa aatatctcgt
     2221 tcatttaatt tcttgatcat atgagtttta attcctgctt cttctaacat atcaaagata
     2281 atgcaagaaa ttgtattgtt taaaactccc ttaccttcaa actgatcatg ttttacacca
     2341 tttccagcag ttgcgtcatc tttataatga atcaaatatt catcttcttt atctgttttg
     2401 taaacttgtt ttgcttttcc ttcatataat aattctgcca ttttgatttc tcctcttatc
     2461 ttttatttat attttgcaat aacttcttca ttagcactca ttgcttgctt ttccatatct
     2521 ttacgatatg ctaataactt ttctttaatc ttatcatgtt tgattgccat gatttgtaac
     2581 gccaaatatg cagcgttctt gctcccgtta attgccacag tagccacagg aattcctgaa
     2641 ggcatttgaa cgattgatag taacgcatcc ataccatcta atgtagcccc gctaattggt
     2701 accccgataa ctggcaatac tgtttgagaa gcgacaactc caggtaaagc tgctgccatt
     2761 ccagccgctg taataattac ttcggttcca tcagtttctg tttctttaat aaatgtactt
     2821 aatcctaaat gtgcccgatg agctgaaaga catcttacat ttacttcaac accatagtct
     2881 ttcaaaattc caattgctgg ttcaacctta cttaaatcgc ttgtagaacc cataataatt
     2941 gctactttca taatttcttc ctttcttttt aaaccactaa aagtgacaat gagtcacttt
     3001 tagaataagc ctactatctt accatcttta tcaatgtcca tattaaccgc tgccggacgt
     3061 tttggtaatc ctggcattct cataatactt ccagagatag ctactaaaaa tcctgctcca
     3121 gcagatggga taagatcatt aatcttaatc gtaaatcccc tagggcgacc aagcagcgtc
     3181 ggttgatcag ataaagagta ttgggtctta gctacacaga ttggtaaatc agctaatcct
     3241 tgtgcttcgt actttttcat ctttgttaat acttttttat caaacgctac tccgtcagca
     3301 ccatagattt ctttcgcgat agtttcaatt ttttctttga ttgataaatt taaatcatat
     3361 aatggttgat aattagcttc tttagtatct aagatttcta atagtttttc agcaagctcg
     3421 attcccccat cagcaccttt ttcccatact ttagaaatag ctacatcaac acccatttta
     3481 ttacatgtat cttttaataa ttgcaactct gcttcactat cagttggaaa agcattgatt
     3541 gctaccactg aaggcaggtt atatttcgca atattttcaa tatgtttttc taaattttta
     3601 attccttttg ctaaagcttc aagattttct gtgcctaact cttttttatc aacaccacca
     3661 tgcattttta gcgctctaac agttgcaacg atcacaactg cttgaggatc aagattagct
     3721 tgacgacatt ttatatctaa gaatttttct gctcctaaat cagcaccaaa tcctgcttct
     3781 gtaattgcat aatcacctaa tttaatagct aatttagttg ccatcactga gttacatcca
     3841 tgagcaatat tagcaaacgg tcccccatga acaaagaccg gcgtatgatc taatgtttgt
     3901 actaagtttg gtttaattgc gtcttttaat aataaagtaa ctgcaccagt tgcttcaata
     3961 tcatttactg taactggatt tccagcatag tcataagcaa cgatcatttt acctgctcgt
     4021 tcttttaaat cttcaaggct cgttgccaaa cataaaattg ccattacttc actagcgaca
     4081 gtaatatcaa aaccgtcttc tcgaggcatt ccattgactt ttccgccaag accacagata
     4141 gtattacgta aagcgcgatc atttaaatca acaactcttc tccaaacaat attacgtaca
     4201 tcgatattta gaggatttcc ctgatgaata gagttatcaa gcattgccgc aataagatta
     4261 ttagcagcag tgattgcatg gatatcacca gtaaaat
//
LOCUS       DAOMTA010000179         4279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_623_length_4279_cov_2.506392, whole genome shotgun sequence.
ACCESSION   DAOMTA010000179 DAOMTA010000000
VERSION     DAOMTA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_4279_cov_2.506392"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLK68_06115"
     CDS             <1..261
                     /locus_tag="PLK68_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM90021.1"
                     /translation="NSMGPIIVSFTMLVPSAIFYEATLGFLGIGLSAPQASWGTLAND
                     ARAMISSQPLQVVWPILAICLTMLALNFIGDGLGDALDPKKK"
     gene            277..1287
                     /locus_tag="PLK68_06120"
     CDS             277..1287
                     /locus_tag="PLK68_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90022.1"
                     /translation="MKMLEVNDLTTEFSTENGTVKAVRDVSFYVDKGEVLGVVGESGS
                     GKSQTMFSIMGLLAGNGSVTNGSIKIDGEEIAPTHFDSRKKYEETMDRIRGNDMAMIF
                     QDPMTFLNPTLRIETQLIEPIINHNPKISKKEARDKAIDLMRKVGIPSPEDRIRQYPH
                     QFSGGMRQRIIIAIALACDPKVIIADEPTTALDVTIQAQVLDLIGSLKDEIDSSIIMI
                     THDLGVVASICDRIAIMYGGKIVETGTTDEIFYNPQHPYTKGLLSCIANPEDLEKKEL
                     HPIPGSPPDLLNISEGCPFLDRCENAMNICVDHMDEYRNFSSTHCSSCWLNNPYLKRK
                     GE"
     gene            1291..2265
                     /locus_tag="PLK68_06125"
     CDS             1291..2265
                     /locus_tag="PLK68_06125"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRM90023.1"
                     /translation="MSEPILKVKGLKVHFPVSGGFLAKKQVVKAVDGVDFEIAPGETF
                     GLVGESGCGKSTTGRALVKIYDPTEGQVIFEGEDITKIKGAKLKEFRRDMQMIFQDPY
                     ASLNPRMTVGEIIREPMDIHGVYDTKEEREKRVRELLEIVGLKPDHIRRYPHEFSGGQ
                     RQRIGIARSLALNPKFIVCDEPISALDVSIQAQVINLLEKIQNEMGIAYLFIAHDLSM
                     VKHISDRIGVMYLGNMVEIGDADDIYHEPLHPYTQALLSSVPIPDPKVARNKKRIVLE
                     GELPSPINPPSGCVFRTRCPKATERCAQEKPALKTVGNRQVACFLYEK"
     gene            2283..3941
                     /locus_tag="PLK68_06130"
     CDS             2283..3941
                     /locus_tag="PLK68_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM90024.1"
                     /translation="MKKLLAYGLSFLMALTCLTGCGGGSEADLRVAVGKDTADLDPAI
                     VDDSVTANILAQVYQGLYTLDTDGNVITNLATDMPEISEDGLTYTIKISGDTKWSDGE
                     KVKASDFVYAWKRAVTTGGYYTQFIWQYIDGTSHMVKDKKTGKETDTPYTTMDELKDF
                     GATALDDTTIQIKLKSKCAYFTSLLTNTVFYPVREDYLKENAEDITKSSWGNNSDVPY
                     NGAFKVTSVNTKDEVFLEKNDEYYDAKNVTLNSISFKVMSDMDAQTSAFEKGDLDFAT
                     SCNIDTINSKDELKKQSWKIDPFVCNYYVLINAGDENTREELKDADIRNAIGLSINRA
                     NVLKALGYGDNAYELNGLIPKGIPGATGDFREEQDAVEKLATYNLDEAKAIMTSKGYS
                     ESNMLKLTYTYNDNTMHKNVAQAMQASMKEAYIDLTLSATEGEAFFDARDKGDFEICR
                     HAMTADFIDPMAYLSMYIGSTTPGNTVDDAKYEELVAAANAIDDKTERMNALHAAENY
                     LVGEQHYVIPLFGYAEPYLMSSKVTGVTHSPEGHYQLAYAKVEK"
     gene            3992..>4279
                     /locus_tag="PLK68_06135"
     CDS             3992..>4279
                     /locus_tag="PLK68_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90025.1"
                     /translation="MKEIRSVKEQYQIKDACLKERLELILPKVMKEYDVDMWISASKE
                     YNEDPLFHAITPANYPTARRISLFAFVKEGDNIHRFSLCMPSEELAPYYTSY"
BASE COUNT         1472 a          621 c          854 g         1332 t
ORIGIN      
        1 aatagtatgg gaccaattat tgtttcattt acaatgttag ttccaagtgc aattttctat
       61 gaagcaacat taggattctt aggtatcggt ttatcagctc cacaagccag ctgggggact
      121 ttagctaatg atgcacgggc gatgatcagt tcacagccat tacaggttgt atggccaatt
      181 ttagcaatct gtttaactat gttagcttta aactttattg gtgatggttt aggggatgcc
      241 cttgacccta agaagaagta ggaggggaat tatagaatga agatgttaga agtaaatgac
      301 cttactactg aatttagtac cgaaaatgga acagttaaag ctgttcgtga tgtatcattt
      361 tatgtagata agggcgaagt gttaggtgtt gttggtgaat ctggttcagg taaatcacaa
      421 acaatgttct caattatggg attactagct ggaaatggta gtgtaactaa tgggtcaatt
      481 aaaattgatg gtgaagaaat agcaccgact cattttgata gtcgtaaaaa atatgaagaa
      541 acaatggatc gaattcgtgg taatgatatg gcaatgatct tccaagatcc aatgactttc
      601 ttaaatccga cattaagaat tgagactcaa ttaattgaac cgattattaa tcataatcca
      661 aaaatttcaa aaaaagaagc acgtgataaa gcgattgatt taatgcgtaa agtaggaatt
      721 ccttcaccag aagatcgaat tcgtcaatat cctcatcaat tctctggtgg gatgcgtcaa
      781 agaatcatca ttgcaatcgc tttagcgtgt gatccaaaag taatcattgc tgatgaacct
      841 acaacagcgt tagatgttac aatccaggcg caagtattag atttgattgg aagtttaaaa
      901 gatgaaatcg attcaagtat tatcatgatt actcatgatt taggagtagt tgctagtatt
      961 tgtgatcgta ttgctattat gtatggtggt aaaattgtag aaactggaac tactgatgaa
     1021 attttctata atccacaaca tccatataca aaaggattac tttcatgtat tgccaatcct
     1081 gaagatttag agaaaaaaga attacatccg atccctggat caccacctga tttattaaat
     1141 atttcagaag gatgtccatt ccttgatcgt tgtgaaaacg caatgaatat ttgtgttgat
     1201 cacatggatg agtatcgtaa tttttcaagt actcactgca gctcatgctg gttaaataat
     1261 ccatatttga aaaggaaagg ggaatagaca atgagtgaac caattttaaa agtaaaagga
     1321 cttaaagttc atttcccagt atctggtgga ttcttagcta aaaaacaagt tgtaaaagca
     1381 gttgatggag tggattttga gattgctcct ggtgaaactt ttggcttagt tggagaatct
     1441 ggatgtggta aaagtactac tggacgtgct ttggttaaaa tttatgatcc aacagaaggg
     1501 caagtcattt ttgaaggtga agatattaca aaaattaaag gagctaaatt aaaagagttc
     1561 cgtcgtgata tgcagatgat ttttcaagat ccatatgcaa gtttaaatcc acgaatgact
     1621 gttggggaaa ttattcgtga accaatggat attcatgggg tttatgatac aaaagaagaa
     1681 cgcgaaaaaa gagttcgtga attattagag atcgttggtt tgaaaccaga tcacattcgt
     1741 cgttatcctc atgagttctc aggaggacaa agacaaagaa ttggaatagc aaggtccctt
     1801 gcattaaatc caaagtttat tgtatgtgat gaaccgattt cagctttgga tgtttcgatt
     1861 caagcacagg ttatcaattt acttgaaaag attcaaaatg aaatgggaat tgcatattta
     1921 tttatcgccc acgatttaag tatggttaaa catatttctg atcgtattgg ggtaatgtat
     1981 ttgggaaata tggttgaaat tggggatgct gatgatattt atcatgagcc acttcatcca
     2041 tatactcaag cgctgttatc ttcggtacct attccggatc ctaaggtagc acgcaacaaa
     2101 aaacgtatcg tattggaagg tgagcttcca agtccgataa atccgcctag tggatgcgtc
     2161 ttcaggacac gctgtccaaa ggcaactgag agatgtgcac aagagaaacc agctttaaaa
     2221 acagttggaa atcgtcaagt ggcatgtttc ttatatgaga aatagttgaa aggggaaaaa
     2281 acatgaaaaa actattagct tatggtctta gcttcttaat ggctctaact tgtttaacag
     2341 gttgtggtgg cggaagtgaa gccgatttac gagttgcagt aggaaaagac actgctgact
     2401 tagatcctgc aatcgttgat gattcagtaa ctgctaacat tttagcgcaa gtttatcaag
     2461 gtttatatac acttgatact gatggtaatg taattactaa tttagcaact gatatgcctg
     2521 aaatcagtga agatggtctt acttacacaa ttaagatttc tggggatact aaatggagcg
     2581 atggagaaaa agtaaaagct tctgatttcg tatatgcttg gaaacgtgcg gtaactactg
     2641 gtggttacta cacacaattt atttggcaat atattgatgg tacttctcat atggtaaagg
     2701 ataagaaaac aggtaaagaa actgacactc cttatactac tatggatgaa ttaaaagact
     2761 ttggagcaac tgctttagat gatacaacta ttcaaattaa gttaaaatct aaatgtgctt
     2821 actttacttc tttattaact aacactgtat tctatccagt tagagaagat tatttgaaag
     2881 aaaatgctga agatattaca aaatcttctt ggggtaacaa ttctgatgtt ccttacaatg
     2941 gagcattcaa agtaacttct gtaaacacta aagatgaagt gttcttagaa aaaaatgatg
     3001 aatattatga tgctaaaaat gttactttaa attctatttc ttttaaagta atgtcagata
     3061 tggatgcaca aactagtgcg tttgaaaaag gtgatttaga tttcgctact tcttgtaaca
     3121 ttgatactat caatagcaag gatgaattaa agaaacaatc ttggaaaatc gatccttttg
     3181 tatgtaacta ctacgtatta atcaatgctg gtgatgaaaa tacaagagaa gaattaaaag
     3241 atgctgatat ccgtaatgca atcggattat caattaaccg tgctaatgtt ttaaaagcat
     3301 taggatatgg tgacaatgct tatgaattaa atggattaat tccaaaaggt attcctggag
     3361 caactggtga cttccgtgaa gaacaagatg ctgtagaaaa attagctact tataacttag
     3421 atgaagcaaa agctatcatg acttctaaag gttatagtga aagtaatatg ttaaaattaa
     3481 catatactta caatgataac actatgcata aaaacgttgc tcaagcaatg caagcatcta
     3541 tgaaagaagc ttatattgat cttactttaa gtgcaactga aggtgaagca ttctttgatg
     3601 ctcgtgacaa aggtgatttc gaaatctgtc gtcatgccat gactgctgac ttcatcgatc
     3661 caatggctta tttatcaatg tatattggtt caactactcc aggtaacaca gtagatgatg
     3721 ctaaatacga agaattagta gctgctgcta atgcaattga tgataaaact gaacgtatga
     3781 atgctttaca tgctgcagaa aactatttag ttggtgaaca acattatgta attccattat
     3841 ttggatatgc tgaaccatac ttaatgtctt ctaaagtaac aggtgtaact cattcaccag
     3901 aaggacatta tcaattagct tatgctaaag ttgaaaaata agaaataaaa tagggtacac
     3961 tttgtgtacc ttatttttct aggaggaaac tatgaaagag attagatcag taaaagaaca
     4021 atatcaaatt aaagatgctt gtttgaaaga acgattagaa cttattttac ctaaagtaat
     4081 gaaagaatat gatgttgata tgtggatcag tgcttctaaa gaatataatg aagatccatt
     4141 atttcatgca attacaccag ctaattatcc aactgcacga agaatttcat tgtttgcttt
     4201 tgttaaagag ggggataata tacatcgttt ttctctctgt atgccaagtg aggaattagc
     4261 gccatattat acaagttat
//
LOCUS       DAOMTA010000180         4253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_627_length_4253_cov_1.971415, whole genome shotgun sequence.
ACCESSION   DAOMTA010000180 DAOMTA010000000
VERSION     DAOMTA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_4253_cov_1.971415"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLK68_06140"
     CDS             <1..853
                     /locus_tag="PLK68_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HRM90026.1"
                     /translation="KGTKYQLKGWQMIKAENTQLLVDGKPVENDLTFTATDSKMEVQI
                     EFTFNASELAGKELVTFEELYDVTNPDEPIKIAEHKDIDDEGQTVTITERIITMHTTA
                     SDKATGEKPINADKEVTIVDTVTLDGLETGTQYQLKGWQMIKSENAQLLIDGKPVEND
                     FTFTATDSKMEVQIEFTFNASELAGKELVAFEELYDLSNPDEPIKVVEHKDINDEGQT
                     VTVKEQLESPVTPEESDTSETPSRAGSSPKTGDNTPLAALLAMLGISSAGLIFAAYKR
                     FYKKKAD"
     gene            1088..1402
                     /locus_tag="PLK68_06145"
     CDS             1088..1402
                     /locus_tag="PLK68_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HRM90027.1"
                     /translation="MELKHVIPNMEKTFGHLEFAGENKVEQRRINGRMAVVSRSFNLY
                     SDVQRADDIIVVLPASAGEKNFESEERVKLINPKITAEGYKIGTRGFTNYILSADDMV
                     KA"
     gene            1418..1786
                     /locus_tag="PLK68_06150"
     CDS             1418..1786
                     /locus_tag="PLK68_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HRM90028.1"
                     /translation="MRLANGIIIDKEKTFGVLKFSALRREVHIQNEDGTVSEEIKERT
                     YDLKCKEQGRMIQVSIPATVPLKEFDYNAEVEIINPVADTVATATFQGADVDWYIKAD
                     DIVLKNKPLKKSVSNNITGK"
     gene            1853..2671
                     /locus_tag="PLK68_06155"
     CDS             1853..2671
                     /locus_tag="PLK68_06155"
                     /inference="COORDINATES: protein motif:HMM:NF040403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6414 family protein"
                     /protein_id="HRM90029.1"
                     /translation="MKKFNKIIYFDKETINNILQELNKGNKMIKSDTMSTIKGSGELE
                     VDTQLKLSVPLIKRISFLFSGKLSASYVKQHDNTTTITSTEISEFERLKPKLKHEENV
                     QIKDIENSSTFFRVAGGYLRMIKGGIDEVDVKEFNNVMNSYEGYDTYKIKDNVYVRFN
                     NSAFVSNYKRNDLLTTKMNLYCVFVGEFKHEEFDFIKQINKMQTLITGLETSSSLADI
                     YPPNEESLMLSASQTDVLDQTDVSDQTNNTSDIIKLYDVVYAAIAVGGGHRDEQ"
     gene            2661..3782
                     /locus_tag="PLK68_06160"
     CDS             2661..3782
                     /locus_tag="PLK68_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM90030.1"
                     /translation="MNNRRYHVIVGSKSYFYKKVSNYYGDSVIPTFLDLVKEADECKQ
                     RGISFDNHADNLIIPNDNYHGITEQAHDRIGVLIDELTYDDAEIFIHNPSTVLKHFLD
                     NLQNEEGVVVDYDPEEYYINKNESEFVQNMKNISTNIIGQKNAIKEISKSMWYLTKTK
                     RQKPYVIMLYGNSSLGKTELVREIAEKFYDNKYYEKHLSMFKNDTYSDYFFGEKPNRR
                     SLGYDLLERESNLIFLDELDKCPMHFFSAFYTLFDNVMFKDASYEADISGLLIILTSN
                     YLSEKEIMNNLGLPIYYRIDKFIPFDDFSIDTIHTIVQKEIFDRKEEYEGLYTAEQIY
                     ASVCSEINIKNENARTIKYKVQKVIERLLFKEIEKEFEN"
     gene            4158..4253
                     /locus_tag="PLK68_06165"
                     /pseudo
     CDS             4158..4253
                     /locus_tag="PLK68_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
BASE COUNT         1505 a          675 c          844 g         1229 t
ORIGIN      
        1 aaaaggtaca aagtatcagc ttaaaggctg gcagatgata aaagctgaaa atacacagtt
       61 acttgttgac ggaaagcctg tggaaaatga ccttacgttt accgcaacgg acagcaagat
      121 ggaagttcag attgaattta ctttcaatgc cagcgagctt gcaggcaagg agcttgtcac
      181 ttttgaggaa ttatatgacg tgacaaatcc agacgaacct atcaagatag cggaacacaa
      241 agacattgac gacgaggggc agactgtgac aatcacagag cgtatcatca ctatgcacac
      301 gactgccagt gacaaggcaa ctggtgaaaa gcccatcaat gccgacaaag aggtaactat
      361 cgtcgatact gttactttgg acggtctgga aacaggcaca cagtaccagc tcaaaggctg
      421 gcagatgata aaatctgaaa atgcacagct actgattgat ggaaagcctg tggaaaatga
      481 ctttacattc acagcgacag acagcaagat ggaggttcag attgaattta cttttaatgc
      541 cagcgagctt gcaggcaagg agcttgtcgc ttttgaggaa ttatacgacc tttctaatcc
      601 agacgagcct atcaaggtag tggaacataa ggacattaac gacgagggac aaaccgtgac
      661 cgtcaaggaa caactagaat cccctgtcac accagaggaa tccgatacgt cagaaacacc
      721 tagcagagct ggcagttctc caaaaacagg tgacaataca cctttagctg cactattggc
      781 tatgcttgga atttccagtg ctggtcttat cttcgcagcc tataaaagat tttataaaaa
      841 gaaagctgac taatacggga ggtatcggct gatgaaagca acgtcccgtt caccgcccat
      901 cataaaaaag tcagcgtata tctgacaatc tggttaaatt ctgcttgatg atggcaaatc
      961 tttctgaatg tggattgtca agggtgcaac gcataacgcc tgcccttgat aatccacagg
     1021 cagacagata ttgtcccgac agcagaatga cactatcaat aaaatatagg aagaaaagag
     1081 gtatattatg gaattaaaac acgttatccc taacatggaa aaaacattcg ggcatttaga
     1141 atttgcaggt gagaataagg tggaacagcg taggatcaac ggacgcatgg cggtcgtttc
     1201 ccgtagcttt aacctttatt ccgacgtgca aagagcagat gatattattg tggtgttgcc
     1261 tgcgtctgct ggtgagaaga actttgaatc agaggaaagg gtaaaactta tcaatcccaa
     1321 aatcaccgca gagggttaca aaatcggcac aagaggtttc acaaattata tcctgtcagc
     1381 agacgacatg gtgaaagcat aaggaggtat cgaatctatg agattagcaa acggaatcat
     1441 cattgacaag gaaaaaacat tcggcgtatt gaagttctcg gctctgcgtc gtgaggtaca
     1501 tatccagaat gaggacggta cggtttctga agaaatcaag gaacgcacct acgatttgaa
     1561 gtgtaaggag cagggacgta tgatacaggt aagtattcct gccactgttc cgttaaagga
     1621 atttgactac aacgcagagg tggaaatcat caatcctgtg gctgatacgg tggcaaccgc
     1681 tacttttcaa ggggcagacg ttgactggta catcaaagcg gacgacattg ttttaaaaaa
     1741 caaacctttg aaaaagtcag tatctaataa tataacaggt aaataaatct tatttgtagt
     1801 aatttgagga tatgttataa ttgttatatc ttttaaattg ggagtgatac acttgaaaaa
     1861 atttaataaa attatttatt tcgataaaga aacaattaat aacatattgc aagaattaaa
     1921 taaaggaaat aagatgataa aaagcgatac tatgtctacc attaaaggat ctggagagtt
     1981 agaagttgat acacaactaa aactctctgt ccctttaatt aaaaggattt cttttctttt
     2041 ttcgggtaaa ttatctgcaa gttatgttaa gcagcatgat aatactacga caataacttc
     2101 aacagaaatt tcggagtttg aacgtctaaa acctaaattg aagcatgaag aaaatgtcca
     2161 aattaaagac atagaaaatt catcaacatt ttttagggtt gctggtggat atttaaggat
     2221 gataaaaggt ggtattgatg aggtcgatgt aaaagaattt aataatgtta tgaatagtta
     2281 tgagggttac gatacatata agataaaaga caatgtatac gtaagattta ataattcagc
     2341 ttttgtaagt aattataaga gaaatgattt attaactaca aaaatgaatc tttattgtgt
     2401 atttgtaggt gaatttaaac atgaagaatt tgattttatt aaacaaatca ataaaatgca
     2461 aacactaatt acaggccttg aaacttcttc ttcattagct gatatttatc caccaaacga
     2521 agaaagtctc atgctatctg cttcgcaaac agatgtatta gaccaaacag atgtatcaga
     2581 ccaaacaaat aatacctctg atataataaa actttatgat gtagtttatg ctgcaatcgc
     2641 tgttggaggt ggacacagag atgaacaata ggaggtatca tgtaatagta ggttctaaat
     2701 cttactttta taaaaaggtt tctaattatt atggtgattc tgtaatccct acttttttag
     2761 atttagtgaa agaagctgat gaatgtaagc aaagaggtat atcttttgac aatcatgcag
     2821 ataatctaat aatcccaaat gataactatc atggtataac tgaacaagca catgatagga
     2881 taggtgttct aatcgatgaa ttaacgtatg atgatgcaga aatatttatt cataatccat
     2941 ctactgtatt aaaacatttt ttggataatt tgcaaaatga agaaggcgtt gttgttgact
     3001 atgacccaga agaatattat atcaacaaaa atgaatcaga atttgttcaa aatatgaaaa
     3061 atatttcaac aaatatcatt ggtcaaaaaa atgccattaa agaaattagt aaatctatgt
     3121 ggtatttaac aaaaactaaa cgtcaaaaac catatgtaat aatgttatat gggaatagta
     3181 gtttgggtaa aactgaatta gttcgtgaaa tagccgaaaa attttatgat aataaatatt
     3241 atgaaaaaca cttgtcaatg tttaaaaatg atacatactc tgactatttt ttcggtgaaa
     3301 aacctaatag aaggtcttta gggtatgatt tattggaacg agaatcaaac ctaatttttc
     3361 ttgacgaatt agataagtgt cctatgcact ttttttctgc gttttataca ctttttgata
     3421 acgttatgtt taaagatgcc tcatatgaag ccgatatttc tggcttgtta ataattttaa
     3481 catcaaatta tttatctgaa aaagaaataa tgaataatct aggattacca atttattatc
     3541 gtattgacaa atttatacct ttcgatgatt ttagtattga taccattcat actattgttc
     3601 aaaaagaaat attcgataga aaagaagaat atgaggggtt atatactgct gaacaaatat
     3661 atgcttcagt ttgttcagaa ataaatatta aaaatgaaaa tgcgcgaaca attaaatata
     3721 aagtacaaaa agttattgaa cgtttacttt tcaaagaaat tgagaaagaa tttgaaaatt
     3781 aatttttcgg cagtgaattc tatatcattc actgcttttt ttatgcctaa aaaggaggat
     3841 ttatgaaatt acaaaaatgt aaaggacgcc gtattcgagc tagtgataag ttgctggttc
     3901 tacggttttt gattggtcat gtggcactct tctttagtgc tacaatactt ttcttgaata
     3961 tcaagacggt atttcagact gattggaagt cggtaactct tctgcaagat ggcagcgtcc
     4021 atctgacagt cacaccctac atgatagttt ctgtcattgc gtcacttctt atttgtgggg
     4081 tggcagctct tttataccgc agattccagt atggcaggat aaagcagctc tttcaccgtc
     4141 aaaagctggc aaagatgatt ctggaaaacg agtggaaccc agaggtggaa atcataaatc
     4201 ctatggctga tacggtggca accgctactt ttcaaggacc agatgttgac cgg
//
LOCUS       DAOMTA010000181         4232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_632_length_4232_cov_1.882452, whole genome shotgun sequence.
ACCESSION   DAOMTA010000181 DAOMTA010000000
VERSION     DAOMTA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_632_length_4232_cov_1.882452"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLK68_06170"
     CDS             <1..1252
                     /locus_tag="PLK68_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM90031.1"
                     /translation="CIDEEQRFGVKQKEKIKEYRKTIDVLTLTATPIPRTLQMSLMGI
                     RGLSQIETPPKNRQPVQTYVIEKNNVLIKQIIERELARDGQVFYLYNRTSQIANVAYN
                     ITLSVPGARVAVGHGQMDKNELEDVMMRFVNKEFNVLVCTTIIETGIDIPNANTIIVE
                     DADKFGLSQLYQIKGRVGRSNRGAYAYLLYNPTKVLNEEASKRLKAIKEFTELGSGYK
                     IAMRDLAIRGSGDILGGTQSGFIDSIGFEMYMKILQDAINEKMGKEDVEAEKEIKSVN
                     VKVDGYIPHDYVSSDIEKLELYQRLDNAKTISGVDHLKSEFIDYYGKLPEEVSTLVEK
                     RKLDILASTEIIENLAEVKGKMEITFTKGYSQNVKGDQLFELVNRLFTKPVFRQLGGK
                     IVIVLPKGDQWLERINQLITTLNS"
     gene            1375..>1556
                     /locus_tag="PLK68_06175"
     CDS             1375..>1556
                     /locus_tag="PLK68_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM90032.1"
                     /translation="MKRDDEMTSQRFNKTIERKKEIIKAAMQLFSEKGYAQTSMRDIA
                     RTMGVSLGLCYRYFDS"
     assembly_gap    1557..1566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1567..1956
                     /locus_tag="PLK68_06180"
     CDS             <1567..1956
                     /locus_tag="PLK68_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM90033.1"
                     /translation="DLYIEECCNSYLAILHDSTITIKDKIDALFTSIGDEHSNMQYYD
                     FFHRVENEELHEQLSIKLCKYMYPHLLEAVKKAIAAKEIYIENPEALISFIIYGQVGL
                     LSKSNIDHHEVAKLLNQYVNQLMKFNP"
     gene            2128..2619
                     /locus_tag="PLK68_06185"
     CDS             2128..2619
                     /locus_tag="PLK68_06185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM90034.1"
                     /translation="MRCYGEEVLDLGCGTGALMKQVISEDSHRHLTGIDLSSQMIEKA
                     KHQLKNKATLVVGDSENLPFFDQTFDIVYCNDSFHHYPNPQKAIAEIYRVLKIGGTLI
                     IGDTYLPVIARQVMNYFIRFNKDGDVRIYSKKEMISFLEELFHEIKWIKVSNSAYMIK
                     GVK"
     gene            2619..2981
                     /locus_tag="PLK68_06190"
     CDS             2619..2981
                     /locus_tag="PLK68_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90035.1"
                     /translation="MIQATNRKYLRYIYQLLLEGKKVRQIDLALSLGYARSSVSIAIK
                     QLKKAGYIDLIKNNITLTERGSMLAQESLKSYQQVYRWILALGLTSYEARLYADKLES
                     DFDQKFIEMLLKDKRLNN"
     assembly_gap    3051..3060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3061..3434)
                     /locus_tag="PLK68_06195"
     CDS             complement(<3061..3434)
                     /locus_tag="PLK68_06195"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRM90036.1"
                     /translation="MNEINKIYQAHMSHELKLVVDPNHAVLNKDKVEAKVLAFLCELG
                     KVGEEAKVFSFWDNSSANNKEILNYYTDALHMLMSIGFELHVDKLKNYQEINNSNNIA
                     NQLIKVYQSALKVNETYSFEAF"
     gene            complement(3489..4088)
                     /locus_tag="PLK68_06200"
     CDS             complement(3489..4088)
                     /locus_tag="PLK68_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRM90037.1"
                     /translation="MKSVREITTYKWIIKKSEFICTLIPCNDETAIDNIIKEYQEKYH
                     DATHNCIAYIVGTQKRANDNGEPSGTAGLPMLDVLEKNKLTNIIAIVTRYFGGIKLGA
                     GGLTRAYRQSVADALKGAEIVEKFSVPLYKITVDYSYTKKFEHLIKVHHIKCINTEYL
                     DRVSYTCYLENESFLTIIQDLTNNTYTKEYLRHDYIELS"
BASE COUNT         1493 a          543 c          754 g         1422 t
ORIGIN      
        1 atgtatagat gaagaacagc gttttggtgt taaacaaaaa gagaaaatta aagagtatcg
       61 taaaactatt gatgttttga cccttacagc taccccgatt cctcgaactt tacaaatgtc
      121 attgatggga attcgtggat tgtcacaaat tgaaactcct cctaagaatc gtcagccagt
      181 tcaaacatat gttattgaaa aaaataatgt tttaattaaa cagatcattg aacgagaatt
      241 agctcgtgat ggacaggttt tctatttgta taaccgaact agtcagattg ctaatgttgc
      301 ttataatatt actttatcag tacctggggc aagggtagcg gtaggtcatg ggcaaatgga
      361 taaaaatgaa ttagaagatg taatgatgcg ttttgtgaac aaggaattta atgttctcgt
      421 ttgcacaacg attattgaaa caggaatcga tattcctaat gcaaatacga ttattgttga
      481 agacgctgat aaatttggat taagtcagtt ataccagatt aaagggcgcg tagggagaag
      541 taatcgtggt gcatatgctt atttgttata taatcctact aaagttttaa atgaagaagc
      601 tagtaaacgg cttaaggcaa ttaaggagtt tactgaatta ggaagtggat ataaaattgc
      661 tatgcgtgat ttagcaattc gcggttcagg agatattttg ggtggtacac aatcaggctt
      721 tattgattca attggttttg agatgtatat gaagattctt caagatgcta ttaacgaaaa
      781 aatgggtaaa gaggatgtgg aagcagaaaa agaaatcaaa agtgttaatg tcaaagttga
      841 tggatatatt cctcatgatt atgtatctag tgatattgaa aaacttgaac tatatcagcg
      901 tctagataat gccaaaacga taagcggtgt tgatcatcta aaatcagaat ttattgatta
      961 ttacggtaaa ttaccagagg aagtaagtac attagtagaa aaacggaagt tagatatttt
     1021 agcatctact gaaattattg aaaacttagc ggaagttaaa gggaagatgg aaattacttt
     1081 tactaaagga tatagtcaaa atgttaaagg ggatcaattg tttgaattag tcaatcgact
     1141 tttcactaaa cctgtgttta ggcaattagg tggtaaaatt gtaattgtct tgccaaaagg
     1201 tgatcaatgg cttgagcgaa tcaatcaatt gataacgaca ttaaacagtt agcaaacaga
     1261 gtattcttga atagggagat ttgtttttaa agcaaatctc tttttttgtt agaaaaacta
     1321 acaaaaaatg aacaatgttc attgaatgct tgttgatgat agcatataat aagtgtgaaa
     1381 agagatgatg agatgactag tcagcgtttt aataaaacaa ttgaacgaaa aaaagagatt
     1441 atcaaagctg cgatgcaatt atttagtgaa aaaggatacg cgcaaacatc aatgcgtgat
     1501 attgctagga caatgggagt ttcgttaggt ttgtgttatc gctattttga ttcaaannnn
     1561 nnnnnngatc tttatattga agagtgttgt aatagttatc ttgctatttt acatgattct
     1621 actattacga ttaaagataa gatagatgca ttatttactt caataggtga tgagcattca
     1681 aatatgcaat attatgattt ttttcatcgt gttgaaaatg aagaattaca tgaacaatta
     1741 tcaatcaaat tatgcaaata tatgtatcca catttattag aagcagtaaa aaaagctatt
     1801 gcagctaaag aaatatatat tgaaaatcct gaggcattaa ttagttttat tatatatggt
     1861 caagttggat tattatctaa aagtaatatt gatcatcatg aagttgcaaa attattgaat
     1921 cagtatgtta atcaattaat gaaatttaat ccctgactct agggattaaa attaaaacaa
     1981 aaaaatgaac atcgttcatt taaggagggc attatgaata aaaaagaaca atctagataa
     2041 acatttgact tacaggcttc aaaatatgat actacttttt atggaaaaca tgcccgtaaa
     2101 atttatccat atcttttaaa tgaaattatt cgctgttatg gtgaggaagt acttgattta
     2161 ggatgcggga caggagcttt aatgaaacaa gttattagtg aggattcaca tcgacatctt
     2221 acaggaattg atttatcatc acaaatgatt gaaaaggcta aacaccaact taagaataag
     2281 gcgactttag tagtaggcga tagtgagaat ttgccatttt ttgatcaaac ttttgatatt
     2341 gtttattgta atgattcatt tcatcactat ccaaatcctc aaaaagccat tgcagaaata
     2401 tatcgtgttt taaaaattgg gggaacattg attattggtg acacatattt gccagtcatt
     2461 gctcggcagg ttatgaatta ttttattcgc tttaataaag atggggatgt tagaatttat
     2521 tcaaaaaaag aaatgatttc gttcttggaa gaattatttc atgaaataaa atggataaag
     2581 gtatcgaata gtgcttacat gatcaaagga gtaaaataat gatacaagca acaaatcgta
     2641 aatatttacg atatatttat caattattgt tggagggaaa aaaggtacgt caaattgatt
     2701 tggctttatc tctaggatac gcacgttcaa gtgtttcaat tgcaattaaa caattgaaaa
     2761 aagcagggta tattgatctt attaaaaata acattacttt aacagagcgg ggaagtatgt
     2821 tggcacaaga aagtttgaaa agttatcagc aagtttatcg atggatattg gctttagggt
     2881 taacttcata tgaagcaaga ttatatgcgg ataaattaga aagtgatttt gatcaaaaat
     2941 ttattgagat gttacttaaa gacaaaagac taaacaatta attgtttagt ctttttgtga
     3001 actgtaattt gctaaaatcg tatcaaaatc aagaccgatt ttaaaaccta nnnnnnnnnn
     3061 tggaatgctt caaaactgta ggtttcatta actttcaatg cgctttgata aaccttgatt
     3121 agttgattgg caatattgtt ggagttgttg atttcttgat agttcttgag tttgtcaacg
     3181 tgcaattcaa atccgattga catcaacata tgaagggcat cagtataata gttaaggatt
     3241 tctttgttat tggcagatga attatcccaa aagctaaata cttttgcttc ttcaccgact
     3301 ttacctaatt cacaaagaaa agctagtact tttgcttcaa ccttatcttt atttaaaaca
     3361 gcatgattag gatcaacgac caattttaat tcgtggctca tatgcgcttg gtagatttta
     3421 ttaatttcat tcatttaatt atcacctctt aagtattatt atactctttt atgtgacatt
     3481 ttcatgaatt atgataattc aatataatca tgacgcaaat attcttttgt ataggtatta
     3541 ttggttagat cttgaataat agttagaaaa gattcatttt caagataaca ggtataacta
     3601 acccgatcta aatactcggt attaatgcat ttaatatgat gtactttaat taagtgttca
     3661 aattttttag tgtatgaata atccactgta attttatata aaggaacact gaatttttct
     3721 acaatctcag ctccttttaa agcatcagca acactttgac gataagctcg agttaaaccg
     3781 ccagctccta atttaatccc gccaaaatat cttgtaacaa tagcaatgat attagtaagt
     3841 ttattttttt ctaaaacatc taacattggt aggccggcag ttccgctcgg ctccccgtta
     3901 tcattggcac gtttttgggt accaacgata taagcaatac agttatgagt agcatcatga
     3961 tatttttctt gatattcttt aataatatta tcaattgcag tttcgtcatt acacggtatt
     4021 aaagtacaaa taaattctga ttttttaatt atccatttat atgtagttat ttcacgtact
     4081 gatttcatta aatccctcat ttctttatta tcataacaca tattactagc aaaagtatga
     4141 taaatttggt atattagtta cgtaattata gggagggcga gaccatgaaa aaaatagctg
     4201 ttttagcaga tagtggatgc cagttaccaa tt
//
LOCUS       DAOMTA010000182         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_636_length_4227_cov_2.482982, whole genome shotgun sequence.
ACCESSION   DAOMTA010000182 DAOMTA010000000
VERSION     DAOMTA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_4227_cov_2.482982"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLK68_06205"
     CDS             <1..528
                     /locus_tag="PLK68_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90038.1"
                     /translation="IINVCGDNDVLPIGIFNVVKNGYYAFEVSTNELFITSLSYKMGK
                     EEFHSIFRTGIGEYKNKSVFSTGFGFGSIVTIKGNHKLNMELICDALHYDWKWGDNKL
                     NLLNQFNLNYQYQVTKHFAIKAGPSFKTFVSNQKENGEFVDVIRMPHTMFEHYGEKYK
                     VAGWMGFNIGAIFSL"
     gene            632..1150
                     /locus_tag="PLK68_06210"
     CDS             632..1150
                     /locus_tag="PLK68_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRM90039.1"
                     /translation="MFKQNYEPLCGFVFGIIKDYDTSEDVIQELFVKFWEKRNDIPDE
                     ISIKPYLYRAARNLALNRIKHFEIKNNFKLFNQEKISDAEQKTCNPAEASELQKMIDK
                     AINNLPPERKKIFLMSRKDGLKYKEIAEELNISIKTVENQMGKALFTLRTELSDYLPT
                     IIIAIYIGTKIS"
     gene            1237..2229
                     /locus_tag="PLK68_06215"
     CDS             1237..2229
                     /locus_tag="PLK68_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRM90040.1"
                     /translation="MNSQHSHIDEKAIAKYLSGEANATERNEMMDWIESSDKNLEDFI
                     RYEKLWSKSSVRKSYNIQKAWQKVNKRISKEKRRFNKYYLSATAVILIVILVQFFNTK
                     PVVSLQQFAVASANSILNSTLPDGSSVMLSKHSKIEYAFDSQKQIRFAKLYGKAFFNV
                     KRDTKHRFIVETNRGGVEVLGTKFTINEMKNKDVMVYVLSGRVKVFLVRAPKDTVSLI
                     ITNGEKAMIMTKNDTIVKQIQKVSSSYTINKPYTVNRSITFHNMDLSTVMAKLGKCYS
                     ANIKIDDSVDKKLRFSSSFKNNSLNEILTVITQTLNIDYTKKGNTYYITNGNNE"
     gene            2222..4051
                     /locus_tag="PLK68_06220"
     CDS             2222..4051
                     /locus_tag="PLK68_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90041.1"
                     /translation="MNNKIRYFILILMLIFALQRSTSQVDLHQKISINVRNFFIPQTL
                     HVIEHNYDFFFAYNSRQIPADDRISINKRNIALKRALDEILSNRFAYTSIGNHIILRL
                     KATNTETEDFIILGVVKSADGLPLDSAVVYAVKENKAVITNNDGAFHLRLKEPSQYIH
                     ISCPKYKDTLLIARTLNSEKIIELNREFPNAISPLGISGLDVSSSQTQFEKIPLVRNL
                     VPQSALYFNRQVKTNRLIPIQVSFLPGMGSQQLTKGLNINRFSLNILAGYSKGVSGFE
                     IGSLANIAGGNIRGGQIAGISNIVNGQVNGLQLAGIFNYTFNQVNGTQIAGIGNISNN
                     DVEGLQLAGVSNNNKGKINGAQISGVLNTNLRKITGLQLAGVVNIQADKISGCQLSGI
                     YGIASDIKGVQLSGIMSHTTHDVSGVQISSILNKARKLNGVQLGIVNIADSIEKGVQV
                     GLFNIVKNGYRAFEFTKDETYYCNFMYKTGGKKLYSILNAGIMKDKVGVGYGIGYIQP
                     IYKELSINMDAIYSALLATNTSDTYLGEIMNFRLGVNYRIQKHLTLTGGLSFNMYDPN
                     KENESSGSAGRISFVGNSRYKILQAFQNNTKAMWFGWFFGVRF"
BASE COUNT         1543 a          666 c          765 g         1253 t
ORIGIN      
        1 attataaacg tttgtggaga taatgatgtt ctacctattg gtatatttaa tgtggttaaa
       61 aacggatatt atgccttcga agtttctact aatgaacttt ttattacaag tttatcctat
      121 aaaatgggta aagaagaatt tcattccata ttcagaactg gcattggcga atacaaaaat
      181 aaatcggtat ttagcacagg atttggtttt gggtctattg ttacgataaa aggtaatcac
      241 aaattaaata tggaactgat ttgcgatgct cttcattatg attggaaatg gggtgataac
      301 aaattgaatc tattgaatca attcaatttg aattatcaat atcaagtcac aaaacatttc
      361 gccataaaag ctggaccgag ttttaagaca tttgtaagta atcaaaaaga aaatggagaa
      421 tttgttgatg taattaggat gccacatacc atgtttgaac attatggaga aaaatataaa
      481 gttgcaggtt ggatgggatt taatatagga gccatatttt ccctataaaa taaattagaa
      541 caaaagaaat attgtacctt tgtagggaat aaatatcaac ttgatggtag ctcttttaaa
      601 tatctggcat acaaaagagg gattcgaaat tttgtttaaa cagaattacg aacccctttg
      661 tggctttgta ttcggtataa tcaaggatta cgatactagt gaagatgtta ttcaagagct
      721 tttcgttaaa ttctgggaaa agcgaaatga tattccagat gaaatctcaa taaagcccta
      781 tttatatcgc gcggcgcgaa atctggcact gaatcggata aaacatttcg agataaaaaa
      841 taatttcaaa ttgtttaatc aagaaaaaat atccgatgcc gaacaaaaga catgtaatcc
      901 tgcggaagca agtgagttgc aaaagatgat tgacaaagca ataaataatt tgcctccaga
      961 acgtaaaaag atttttttga tgagtcgtaa agatggatta aaatacaaag aaatagctga
     1021 agaattaaat atatcaataa aaacggttga aaatcagatg ggaaaggctt tgtttacgct
     1081 acgaaccgaa ttatcagatt atttaccgac aataattatt gcaatttata taggcacaaa
     1141 aataagttga atatgaaaaa tcataagttg aaaaaatata atattataaa tgttacatgg
     1201 catataaaat aaaaaattga acggacataa atttaaatga acagccagca ttcacatatc
     1261 gatgagaagg ccatcgcgaa atacctttcg ggtgaagcta atgctaccga aagaaacgag
     1321 atgatggatt ggatagaatc ttcggataaa aatttggaag attttatccg atatgaaaaa
     1381 ctgtggtcta aatcatctgt tcgcaaatct tataacattc aaaaagcttg gcagaaggtc
     1441 aacaaacgca tttcaaagga gaaacgccgt ttcaacaaat attatcttag tgccactgcc
     1501 gttatcctaa ttgtaattct tgtccaattt ttcaatacca agccagtcgt ttcattacaa
     1561 caatttgcag tagcgagtgc gaatagtata ttaaattcta cattaccaga cggttcttct
     1621 gtcatgcttt cgaaacattc gaaaatagaa tacgcatttg attctcaaaa acaaattcgc
     1681 tttgcaaaac tctacggaaa agctttcttc aatgttaaaa gagatactaa gcatagattt
     1741 attgttgaaa ccaatcgtgg tggtgtggaa gttttaggca caaagttcac tataaatgag
     1801 atgaaaaaca aagacgttat ggtctatgtt ttgtctggaa gagttaaagt ttttttagtg
     1861 cgcgctccta aagatactgt atctctgata attaccaacg gagaaaaagc tatgattatg
     1921 acaaaaaatg acacaatcgt caagcaaata cagaaagttt cgagttctta cactatcaat
     1981 aaaccttata ccgtcaacag atcaatcact tttcacaata tggatctgtc aactgtaatg
     2041 gcaaagttag gaaaatgtta ttctgcaaac ataaagattg atgattctgt ggacaagaaa
     2101 cttcgttttt cttcctcatt caaaaataat tcgttgaatg aaatattaac ggtaataacg
     2161 caaacgctga atattgatta cacaaagaaa ggaaacacgt attatataac gaatggaaac
     2221 aatgaataat aaaattagat actttatatt gattctaatg ttgatttttg cgttgcaaag
     2281 atcaacttca caagttgatt tgcaccaaaa aatatccatt aacgtgcgaa attttttcat
     2341 tccacaaacc ttgcacgtta tcgaacataa ttatgatttc ttctttgcat acaattctag
     2401 acaaattcca gcagatgaca gaatttcaat caataaaaga aatattgcgt tgaaaagagc
     2461 attggatgaa atattgtcaa atcgttttgc ttacacaagc attggaaacc atattatttt
     2521 acgcctaaag gcaacaaaca cagaaactga agattttatt atcttggggg ttgtaaaaag
     2581 tgctgatggt cttcctttgg attctgctgt tgtatatgcc gtcaaagaaa acaaagctgt
     2641 aatcacaaat aatgatggag cattccatct cagattgaaa gaaccatcac aatacattca
     2701 tataagctgt cctaagtaca aagatactct cctgattgct agaaccttaa atagtgagaa
     2761 aataattgaa ctgaatcgag aatttccaaa tgccatttca cctttaggta ttagtggatt
     2821 agatgtatct tcctcacaaa cacaatttga aaaaataccc ttagtacgaa atttagttcc
     2881 acaatcagcg ctatacttta acaggcaagt aaagacaaac cgtttgatac ctatccaagt
     2941 ttccttcttg ccaggcatgg gctcccagca gctaaccaag ggcttaaata tcaatcgttt
     3001 ctcattgaat attttagctg gttattctaa aggagtttct ggttttgaaa taggaagtct
     3061 tgctaatatc gctgggggaa atataagggg cggacaaatt gctggtatat caaacatcgt
     3121 taacgggcag gttaatggct tgcaacttgc tggtattttc aattatacat tcaaccaagt
     3181 caatggaaca caaattgctg gaatcgggaa cattagcaac aatgatgtag aaggattaca
     3241 acttgctggt gtgtcaaaca acaacaaagg taaaattaac ggagcacaaa tcagcggtgt
     3301 cttgaataca aatttgcgaa agataactgg acttcagtta gccggtgtcg ttaatattca
     3361 ggccgacaag atatcaggat gccaactttc tggaatttac ggaatagctt ccgatatcaa
     3421 aggagttcag ctttctggta taatgagtca cacaacacac gatgtttctg gtgtacaaat
     3481 cagctcaata ttaaacaaag ctagaaaact aaatggagtg caattgggta tcgtcaacat
     3541 tgcagattcc attgaaaaag gagttcaagt cggtttattc aacatcgtca aaaacggata
     3601 ccgggccttt gaattcacaa aagatgaaac atattactgc aatttcatgt acaaaactgg
     3661 agggaaaaaa ctatattcca ttctgaatgc aggaatcatg aaagacaaag tcggcgttgg
     3721 atatggtatc ggatacatcc aaccaattta taaagaacta tctataaaca tggacgcaat
     3781 ttatagcgcg ctacttgcga caaacacctc cgatacttat ctgggagaaa taatgaattt
     3841 tcgattgggt gtcaattatc gtattcaaaa acatttgacg ctgacaggcg gcttatcttt
     3901 caatatgtat gatcctaaca aggaaaatga gtcaagtggt tctgcgggca gaatatcatt
     3961 cgtaggaaac agtcgttata aaattttaca agcctttcaa aacaacacga aagcaatgtg
     4021 gtttggctgg ttttttggtg tgagatttta aacttataga catctgttag aattttaaaa
     4081 aaagaatagg agcaatggag atgtactaac gtcgttccta aattcaacga tatatgccaa
     4141 aataaattaa cgtgagttcg atgaattata agtgctgaat tcaagcgtat aatgcaaccg
     4201 cattgtaatc caaattagtt aattgtt
//
LOCUS       DAOMTA010000183         4220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_641_length_4220_cov_2.345018, whole genome shotgun sequence.
ACCESSION   DAOMTA010000183 DAOMTA010000000
VERSION     DAOMTA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_4220_cov_2.345018"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1027
                     /locus_tag="PLK68_06225"
                     /pseudo
     CDS             26..1027
                     /locus_tag="PLK68_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            1118..2053
                     /locus_tag="PLK68_06230"
     CDS             1118..2053
                     /locus_tag="PLK68_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90042.1"
                     /translation="MEVKKSIVPQIMTILLVALMILMSEVFHEKEFIFPEITALTIGA
                     WLAPKQVWKTNKIKLVFLIAAYASLGIILVKYVDIDIYFKILIGFVVCVTGLFLSKTT
                     FAPLISATILPIIINSESWLYPLFATAMSILIVIGQQYLEKGGYRSVQEYHPVTAAVK
                     ETYSLNLKRLCALALTAFIALQLKLPFLIAPPLIVAFVELSSNHPKLRHNSIKLAFVT
                     FICAFSGAYERILISEIGDLPLTISAIIIVMTMLSIMKITKLYFPPAGALAILPLLIE
                     SGKLIVYPFVIIGGFVIFTVFAFIITKTPLNSLSK"
     gene            2114..>3202
                     /locus_tag="PLK68_06235"
     CDS             2114..>3202
                     /locus_tag="PLK68_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRM90043.1"
                     /translation="MSDFIKEVEKRRTFAIISHPDAGKTTLTEKFLLYGGAIQQAGTV
                     KGKKNSRHATSDWMEIEKQRGISVTSSVLQFNYDGFCINILDTPGHQDFSEDTYRTLM
                     AADCAVMVIDASKGVEDQTRKLFKVCTMRNIPIFTFINKMDREAQDPYNLMEEIEQEL
                     GIDTCPVNWPIGSGKQFKGVYERHDQEVIRFIPVDGGRKEVDTKIMKYDDPELINELD
                     EELYNKLQEDIELLDMAGNDFDLEKVRQGKLSPVCFGSALTNFGIEPFLKHFLEMTTP
                     PLPRSTGEKTIDPFSEDFSAFVFKIQANMNKAHRDRMAFFRICSGKFTKGMEVYHYQG
                     GRKIKLNQSKQLMADERQEVDEAYAGDII"
     assembly_gap    3203..3212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3213..3707
                     /locus_tag="PLK68_06240"
     CDS             <3213..3707
                     /locus_tag="PLK68_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRM90044.1"
                     /translation="VFDPGIFSIGDTITTGKAKFAFEGIPTFAPEHFSLIRNKDTMKR
                     KQFVKGVEQIAKEGAIQIFTELGGGMEEIIVGVVGVLQFEVLEYRLKNEYNVEIIKQP
                     LPYQYVRWVKEGCDLKALNLSSDTKKVQDLKGQHLLLFTNDWGVRWATEKNPDLELLE
                     FSKN"
     gene            3764..4012
                     /locus_tag="PLK68_06245"
     CDS             3764..4012
                     /locus_tag="PLK68_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90045.1"
                     /translation="MITIANTGKRRVCRCIKTMNIVIGKEQRDLFTKGQVYDCVIRDS
                     GQLQVNYKIYGKEFDLSCTKEEFEENFVLINKKTGVRK"
     gene            4009..>4220
                     /locus_tag="PLK68_06250"
     CDS             4009..>4220
                     /locus_tag="PLK68_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90046.1"
                     /translation="MIFEDLKLAEFNDFYKVILGNFPSKEIKEYDYMKETFINGDFKV
                     LTLKEDDEIKGILSHYDGGEFAFVDY"
BASE COUNT         1410 a          519 c          859 g         1422 t
ORIGIN      
        1 atatctttaa aaagaagcgt ttgctcatgt tccaacgtgg tttaaagaat ttagaaatga
       61 ttatgctgct aaaaaggctg cctttttaac aattattagt gatgatcctg aaggttttaa
      121 aggaatcgat cctgcaaaga tcgcattgtg gtcaaaaagt gtatcaactg cttgtaaacc
      181 attttatgat agccttgatt tggggataaa tacatggtgc attgttgctg gcagttcaat
      241 taaatgggca aataaagtat atcctgatat gtctgatagt gaggcagtag aggcgttgtg
      301 gaatgctatt tttaaagcgg ttaaagtcgt agatgatgac cctttgactg cctggcagga
      361 acatcgtaaa agttttgaag ctcgagtagc atatttaaat aaattgaatt taaatacatt
      421 gacttatact aattcgcttg gaacaaacct aactgttacc ttaaacgagg gttacctatt
      481 tgctggtggg ggttcttata cgactaaagg acaatatttt tttccaaata tgccaaccga
      541 ggagattttt acaagtccat atcgtaatgg gactaatggg gttgtgtata gttctctgcc
      601 attaaattat aatggtaatt tgattgatga atttaaaatg gaattcaaag atggtcgaat
      661 tattgatttt gatgcaaaaa tgggtaaaga ggttttaaaa gagatgttga gtattgatga
      721 aggtagtctt tatttgggtg aaattgcctt ggtaccatat gattcgccaa tttctaatat
      781 gaaaacatta ttctataata ctttatttga tgaaaatgct tcatgtcatt tagcaattgg
      841 aaaaggcttt agtgaatgta ttcaaggtgg tttaacaatg actaaagaac aattgttgga
      901 aaagggagtc aatgattcat taactcatgt tgattttatg attggtacta gtgatttgaa
      961 aattgtggga gaaacaaaag atggtcaagt agtaaggata tttgaaaacg gaaattttgt
     1021 attttaagaa atgacgatat aacggttagg ttatatcgtt ttcttattta ctatactcgc
     1081 tttttgtaat agaatgagtt ctattgaggt gaaaatgatg gaagtaaaga aaagtattgt
     1141 tccccagata atgacgatct tgctagtagc tttaatgatc ttgatgtcag aagtgtttca
     1201 tgaaaaagaa tttatttttc cagaaattac ggcattgacg attggagcat ggttagcgcc
     1261 taaacaagta tggaaaacta ataaaattaa attggttttt ttaattgcgg cttatgcgtc
     1321 gttagggatt attttggtta aatatgtaga tattgatatt tattttaaaa ttttaattgg
     1381 ctttgtagta tgtgtgacgg gattattttt atctaaaacg acgtttgctc cattgatatc
     1441 tgcaacgatc ttaccaatca ttattaatag tgagtcatgg ttgtatccgc tttttgctac
     1501 tgcaatgagt attttgattg taatcgggca acaatattta gagaagggcg gctatcgtag
     1561 tgttcaggaa tatcatccgg ttactgcagc agttaaagaa acctatagct taaatttaaa
     1621 acggttatgt gcattagcat taacggcttt tattgcttta caattaaaat taccgttttt
     1681 gattgcccca cccttgatag ttgcttttgt tgaattgagt agtaatcacc caaagttgcg
     1741 tcataattcg attaaattag catttgttac ctttatttgt gcatttagtg gtgcttatga
     1801 aagaattttg ataagtgaga ttggtgattt gccattaact attagtgcaa ttataattgt
     1861 aatgaccatg ttgagcatta tgaaaattac taaattatat tttccgccag ctggtgcctt
     1921 agcaatttta ccgttattga ttgaatcggg aaaattaata gtttatccgt ttgtgataat
     1981 cggtggattt gtaatcttca ctgtctttgc tttcattatt acgaagactc cgttaaacag
     2041 tctgagtaaa tagatttcgg ttgacaaatt cattataaat ctatatgatt aatccttgca
     2101 aggagatata aaaatgagcg attttatcaa agaagttgaa aaaagaagaa cgtttgccat
     2161 catctcccat cccgatgctg gtaagacaac attaacagaa aaattcttat tatacggagg
     2221 ggcaattcaa caggcaggta ccgtaaaagg aaaaaagaac agcagacatg caacaagtga
     2281 ctggatggaa atagaaaagc aaagaggaat ctctgtaaca tcatctgtat tgcaatttaa
     2341 ttatgatggt ttttgtatta atattttaga tacaccaggt catcaggatt tctctgaaga
     2401 tacttatcgt acattaatgg cagctgattg tgcagttatg gttattgatg caagtaaagg
     2461 ggtcgaagat caaactcgta aattatttaa agtttgtacg atgcgtaata tcccaatttt
     2521 tacttttatc aataaaatgg accgtgaagc tcaagatcca tataatttaa tggaagagat
     2581 cgaacaagaa ttggggatcg atacttgtcc agttaactgg cctattggtt ctggaaaaca
     2641 gtttaaaggt gtttatgaac ggcatgatca ggaagtaatc cgttttatcc ctgttgatgg
     2701 cgggcgtaaa gaagttgata ctaaaataat gaaatatgat gatcctgaat taattaatga
     2761 attagatgaa gaattatata ataagctgca ggaagatatt gagttattag atatggcagg
     2821 aaatgatttt gatttagaaa aagtgcgtca aggtaaactt tctccagtat gctttggttc
     2881 tgctttgact aactttggaa tcgaaccatt tttaaaacat ttcttagaaa tgacaacacc
     2941 accattacca cgaagtactg gtgaaaaaac aatcgatcca tttagtgaag atttcagtgc
     3001 ctttgttttt aagattcaag ctaatatgaa taaagcacat cgtgaccgaa tggctttctt
     3061 tagaatttgc tctggtaaat ttactaaagg gatggaagta tatcactatc aaggtggtcg
     3121 taaaataaaa ttgaatcagt caaaacaatt gatggctgat gaacgtcaag aagttgatga
     3181 agcttatgct ggagatatca tcnnnnnnnn nngtttttga tccagggatc ttttcgattg
     3241 gagatacgat tacaacaggt aaagctaaat ttgcttttga aggtattcct acctttgctc
     3301 cagaacattt ttcattgatt cgtaataaag atacgatgaa aagaaaacaa tttgttaaag
     3361 gtgtagaaca aattgctaaa gagggagcaa tccaaatttt cactgaatta ggcggtggaa
     3421 tggaagaaat tattgttggt gtcgttgggg tacttcaatt cgaagtatta gaatatcgct
     3481 taaagaatga atataatgtt gagataatca agcagccgct accatatcaa tatgtgcgct
     3541 gggttaaaga gggatgcgat ttaaaagctt taaatttatc tagtgataca aaaaaagtac
     3601 aagatttaaa aggacaacat ttattattat ttactaatga ttggggcgtt cgttgggcta
     3661 cagagaagaa tcctgattta gagttattgg aattcagtaa gaattagcag ggctaattct
     3721 tacttttctt ttaattatca tgttataatg aatgagaaaa gaggtgatta ctatcgcaaa
     3781 tacaggaaaa cgtcgcgtgt gtcgttgtat caagacaatg aatattgtaa ttggtaaaga
     3841 acagcgggat ctatttacta agggccaagt ttacgattgt gtgattcgtg atagtggtca
     3901 gcttcaggta aattataaaa tatatggtaa agagtttgat ttgtcatgta ctaaagagga
     3961 atttgaagaa aactttgttt taataaataa aaaaacgggg gttagaaaat gatatttgaa
     4021 gatttaaaat tagcagaatt taatgatttt tataaagtta ttttaggaaa ttttcctagt
     4081 aaagaaatca aagaatatga ttatatgaaa gagactttta ttaatggtga ttttaaagtt
     4141 ttgactttaa aagaagatga tgaaattaaa ggtattttat cacattatga tggtggagaa
     4201 tttgcttttg ttgattattt
//
LOCUS       DAOMTA010000184         4217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_643_length_4217_cov_2.583854, whole genome shotgun sequence.
ACCESSION   DAOMTA010000184 DAOMTA010000000
VERSION     DAOMTA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_4217_cov_2.583854"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLK68_06255"
     CDS             complement(<1..844)
                     /locus_tag="PLK68_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM90047.1"
                     /translation="MKNKLWFRYLITIVAVIASSFLQTYAIKVFVEPANLLSSGFTGV
                     AILIDRITSLYGFNFSTSLGLIVLNVPVAILCFKSIGKKFVISSLCQVFLTSFLLRIC
                     TFPPLFNDVILNVFFGGFVYGMSTVIALRGNTSSAGTDFIALYVSNKMGKSIWEYVFI
                     FNALILCIFGYMFGWIYAGYSIVFQFISTKTISSFYQRYKRVTLQITTTHPEQIIERY
                     VKDYRHGVSVVNGYGGYSKNPMSLLHTVVSAYEVQDIVQLMHESDEKAIINVIPTENF
                     FGGFY"
     gene            complement(841..1575)
                     /locus_tag="PLK68_06260"
     CDS             complement(841..1575)
                     /locus_tag="PLK68_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRM90048.1"
                     /translation="MIEICCGSYEDALNAYYGGAKRIELNSALHLGGLTPSIGSLKLT
                     KNTTNLKVICMVRPRGAGFCYTDIEFKQMMIEAKDLLENGADGIAFGFLLKNHEIDIE
                     RTKEMVSLIKSYQKEAVFHRAYDCVKDPYASIEILINLGIDRLLTSGLRAKAADGKDL
                     IKKLQTKYGDKIEILAGSGINSTNGQAIMEYTGIAQIHSSCKDWQNDPTTSGEFVNYC
                     YGPAEHKDDFDMVSKELVEKLVKLGL"
     gene            complement(1591..2691)
                     /locus_tag="PLK68_06265"
     CDS             complement(1591..2691)
                     /locus_tag="PLK68_06265"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRM90049.1"
                     /translation="MEDIILKKVDDIAEQMIKSIIEIVKIDSVETAPLENAPFGSGVK
                     AALDAALDLAASLGFVTTNIDNYIGYASYGESKDYICAIGHLDVVPVGTGWKQPPFSG
                     YVEDGVIYSRGILDNKGPILSCLFALYALKELKLELAHEIRIIFGCDEESGFEDLKYY
                     LSKERPPLMGFTPDCKYPVVYGERGRAIIKICGKKDRLKEFFDLVNNYILNAKNNGER
                     FNIDFQDQEFGLLEVRNYQLELDGDPSIQFSVSYPASVTVETIVNNIKMVVSNFEVNL
                     VGNYDPVKFPLKCKLVKTLVHTYEKVTGDDGTPVTTTGGTYAKLMPNIVPFGPSFPGQ
                     KGIGHQPNEWMTIEDIITNAKIYALSLYNLAK"
     gene            complement(2693..3649)
                     /locus_tag="PLK68_06270"
     CDS             complement(2693..3649)
                     /locus_tag="PLK68_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HRM90050.1"
                     /translation="MWGIIATWRMAVEGISKASQVLAEGGDAGDAIEIAVREVEDFPY
                     YKSVGYGGLPNEEMEVELDAAFMDGDTLDIGAVAAIKDYANPVSIARRLSKEKVNNLL
                     VGEGAEKFAHKEGFERKNMLTDRAKIHYRNRVKEVQALEIKPYSGHDTVGMVCLDTHG
                     KMTSATSTSGLFMKKAGRVGDSPISGSGFYVDSKVGGASATGLGEDVMKGCVAYEIVR
                     LMKDGMHPQAACEKAVNMFDLELKERRGQAGDMSLVAMNNKGEWGVATNIEGFSFAVA
                     TADQEPIVYLTENKDGKCIHTVASQEWLDNYMATRTAPLEEK"
     gene            complement(3708..>4217)
                     /locus_tag="PLK68_06275"
     CDS             complement(3708..>4217)
                     /locus_tag="PLK68_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HRM90051.1"
                     /translation="MIYDQIQSGAGIKDDHMIPLGAKKEPVGPAIMMEQYLKTVDGRV
                     MACLYCGDGYYEANPEEVSRKLCAMINKLKPDVVMCGPAFNYLGYGKMAANIAYDINQ
                     TTDIPAFAAMSKENEETINEFKDKIHIIETPKKGGIGLNESLDGMCKLAKALVDHEDL
                     NPITSKYCF"
BASE COUNT         1349 a          903 c          528 g         1437 t
ORIGIN      
        1 gataaaaacc gccaaagaaa ttttccgtag gaataacatt aattattgcc ttttcatcag
       61 attcatgcat taattgaaca atatcttgaa cctcataagc agatacaacc gtatgaagta
      121 aactcattgg atttttacta taaccaccat aaccattaac aactgaaaca ccatgacgat
      181 aatctttaac atatctttca attatctgtt ccgggtgggt tgttgtaatc tgcaatgtta
      241 cccgcttata cctttggtaa aatgatgaga ttgttttggt tgaaataaac tggaagacaa
      301 ttgaatagcc tgcatatatc cagccaaaca tataaccaaa gatacaaaga atcaaggcat
      361 tgaaaataaa aacatactcc cagatggatt tacccatttt attagaaaca tataaagcaa
      421 taaaatcggt accagctgat gaagtattcc ctcttaatgc aattactgtc gacatcccat
      481 aaacaaatcc accaaaaaaa acatttaaaa taacatcatt aaataacggt gggaatgtac
      541 aaatccttaa taaaaagctt gttaaaaaaa cttggcacaa agaagaaata acaaatttct
      601 ttccaatact tttaaaacat aagattgcta ctggtacatt taaaacaatc agtcctaaag
      661 aagtagaaaa attaaagcca tataatgaag taattcgatc aattaaaatt gctacaccag
      721 taaaaccact agataacaga ttagctggtt ctacaaaaac tttgatagcg tatgtttgca
      781 aaaaagaaga tgcaattact gctacaatcg ttattagata tcgaaaccag agtttgtttt
      841 tcataatcct aatttcacta atttttcaac taattcctta ctaaccatgt caaaatcatc
      901 tttatgctct gccggtccat agcaatagtt tacaaattct ccacttgtag ttggatcatt
      961 ttgccaatct ttacatgaag aatgaatttg agcgattcct gtatattcca tgattgcttg
     1021 tccgtttgta ctgttaattc cacttccagc taatatttct attttatcgc catactttgt
     1081 ttgtaatttt ttgattaaat ctttaccatc agcggcttta gctcgtagtc cgctcgtaag
     1141 caagcgatca atgcctaaat taattaatat ctcaatactg gcatatgggt cttttacaca
     1201 atcataagca cggtggaaaa ccgcttcttt ttgataagac tttattagtg aaaccatttc
     1261 cttggttctt tcaatatcaa tttcatgatt ttttaataaa aatccaaagg ctataccatc
     1321 ggctccattt tctaatagat ccttagcttc tatcatcatc tgtttaaatt caatatctgt
     1381 ataacaaaat cccgctccac gtggtcgaac catgcagatt acctttaagt ttgtagtatt
     1441 ttttgtcaat tttaatgaac caatgctagg tgttagacca cctaaatgta aggcactatt
     1501 caactcaatt ctttttgctc caccataata tgcatttaga gcatcttcat aacttccaca
     1561 acatatttca atcactctgc atccctactt tcattttgct agattatata aacttaaggc
     1621 gtaaatctta gcatttgtaa taatatcttc aattgtcatc cattcattag gttggtgacc
     1681 aattcctttt tgaccaggaa atgatggtcc aaaaggaaca atattaggca tcaacttagc
     1741 atatgtccca ccggttgtag taactggtgt tccatcatct ccagttacct tttcataagt
     1801 atggactaat gtctttacta atttacattt tagcgggaat tttacaggat cataatttcc
     1861 cactaaatta acttcaaaat tgctaactac catctttata ttattgacta ttgtttccac
     1921 tgtgacactc gctggataac taacactaaa ttgaatactt ggatctccgt ctaattctaa
     1981 ttgataattt cgaacttcta ataatccaaa ttcttgatct tgaaaatcaa tattaaacct
     2041 ttcgccatta tttttagcat ttaaaatata attattgacc aaatcaaaga attcttttaa
     2101 tctatccttt ttcccgcata ttttaattat agcccgtcca cgctcaccat aaacaacagg
     2161 atatttacaa tctggtgtaa atcccattaa cggcggtctt tccttactta aataatattt
     2221 taaatcttca aaaccagatt cttcatcaca accaaagata attctaatct catgagctaa
     2281 ttcaagtttt aattctttaa gtgcataaag agcaaataga catgataaga ttggtccttt
     2341 attatctaaa attccccgac tataaatcac cccatcttct acatacccac taaacggagg
     2401 ctgtttccag cctgtcccaa ctggaacaac atctaaatga ccaatagcac aaatataatc
     2461 tttactttca ccataacttg catatccgat ataattatca atatttgttg ttacaaatcc
     2521 caacgaagca gctaaatcca aagcagcatc taaagctgct ttgacccctg agccaaatgg
     2581 agcattttct aaaggagctg tttcaacact atcgattttt acaatttcaa taatactttt
     2641 aatcatttgt tcagcaatat catcaacttt ttttaaaatt atatcttcca tcttattttt
     2701 cttctaaagg ggctgttctt gtagccatat aattatctaa ccattcttga ctagctaccg
     2761 tatgtataca ttttccatct ttattttctg tcaaataaac aattggttct tggtcagcag
     2821 tagcaacagc aaatgaaaat ccttcaatat ttgtagcaac accccattct cctttattat
     2881 tcattgctac caaagacata tcacctgctt gaccacggcg ttcttttaat tctaaatcaa
     2941 acatattaac cgctttttca caagcagcct gaggatgcat tccatctttc attaaacgaa
     3001 caatttcata agctacgcac cctttcataa catcctctcc taagccagtt gcacttgcac
     3061 caccgacttt actatcaaca tagaagcccg atccagaaat cggtgaatct ccgacccgac
     3121 cagctttttt cataaataag ccacttgtag aagtagccga agtcatttta ccatgagtat
     3181 ctaaacatac cattccaacc gtatcatgtc ctgaataggg tttaatttcc aatgcttgaa
     3241 cttcttttac acgattacga tagtgaattt tagcacgatc agtcaacata ttctttcttt
     3301 caaaaccttc tttatgagca aatttttcag cgccttcacc aactaacaaa ttgttcactt
     3361 tttcttttga taatcgtctt gcaatactta ctggatttgc ataatctttg attgccgcta
     3421 ccgcaccaat atctaatgta tcaccatcca taaaagccgc atctaattca acttccattt
     3481 cttcgtttgg caacccgcca tatccaactg acttatagta cggaaagtcc tctacctctc
     3541 taaccgctat ttcgattgcg tcaccagcgt caccaccttc tgctaaaact tgacttgctt
     3601 tactgattcc ttctacggcc attcgccatg ttgcaataat accccacatc taagagttcc
     3661 tcctttattt tttgataaaa ggatgcgtta tccgcatcct ttttagttta gaaacaatac
     3721 tttgaagtaa tcggatttag atcttcatga tcaactaatg ctttcgctaa tttgcacatt
     3781 ccatctaatg attcatttaa gccaatccca cctttttttg gtgtttcaat aatatgaatt
     3841 ttatctttaa attcattaat cgtttcttca ttttcttttg acattgctgc aaatgcagga
     3901 atatctgttg tctgattaat atcataggca atattagctg ccatcttgcc ataacctaaa
     3961 tagttaaatg ctggtccaca cattactaca tcaggtttta acttattaat catcgcgcat
     4021 agtttacgac taacttcttc aggatttgct tcataatacc catcaccaca atataaacat
     4081 gccatcacgc gtccgtcaac tgtttttaaa tattgttcca tcataattgc tggtccaacc
     4141 ggttcttttt tagctcctaa aggaatcata tggtcatctt tgattcctgc accagattga
     4201 atttgatcat aaatcat
//
LOCUS       DAOMTA010000185         4188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_651_length_4188_cov_2.387612, whole genome shotgun sequence.
ACCESSION   DAOMTA010000185 DAOMTA010000000
VERSION     DAOMTA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_651_length_4188_cov_2.387612"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLK68_06280"
     CDS             <1..274
                     /locus_tag="PLK68_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM90052.1"
                     /translation="KLNITKATLSVRLQRLEKLGYLTRTQDKNDKRNYILNITKTGEV
                     FIEATIKIMKEKTMIMFEGVSKEQITVINDVINIMKKNIEKCKGEE"
     gene            274..2502
                     /locus_tag="PLK68_06285"
     CDS             274..2502
                     /locus_tag="PLK68_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90053.1"
                     /translation="MLKLKHYFKKFWAPILLCVGLLFLQSQSELALPDYMSDIVSVGI
                     QAGGFDSAVSDVLSEETYNHLLVLMDEEDQQQFMDAYKLVEPSNLDKDTLDKFPKAKG
                     QNIYKLKDLSEKKLDRLESILVKPMLMVTSIDGMDKNSKEYQEQFGQLPPNMTPYDAL
                     AMMDNTTKAKMFSKIDSQMETMGESTLKIAAGNGVKAEYSRLGCDTDKIQNDYILWSG
                     LKMLAIALAGTVCAVACGFLASKVGAGVSRLLRRDVFEKVESFSNEEFNKFSTASLIT
                     RTTNDITQVQMVVIMFIRIVCFAPMMGIGALIKAFNNTPSMTWIIGLVLLVIFCLIGV
                     TFAIVMPKFKIVQSLIDRLNLTMRENLSGMLVIRAFGNEKHSEERFEKANGDLTNVNL
                     FVNRTMASLMPIMMFIFNVVTLLIVYYGAKQIDLGNIAIGQMMAFMQYAMQIIMSFLM
                     IAMISIMLPRASVAADRFYEVISMEPKIVDPKEPKAFIESKKGLVEFNNVTFKYPGAN
                     EAVLENISFTAKPGETTAFIGSTGSGKSTLINLIPRFYEVTEGNIKIDGVDIRDVNQH
                     DLRDKIGLVPQKGLLFSGTIRSNLTYGAPEATDDELEEVIRVAQAKEFIDNKEERLDS
                     EISQGGTNVSGGQKQRLAIARAIAKNPEIFIFDDSFSALDFKTDAKLRQELDKMVKKT
                     KNTVLIVGQRIASIMGAEQIIVLDEGKIVGKGTHEELMKNCDVYQEIAYSQLSKEELG
                     HE"
     gene            2495..>4188
                     /locus_tag="PLK68_06290"
     CDS             2495..>4188
                     /locus_tag="PLK68_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90054.1"
                     /translation="MSNQPKRNGPKFGPGGMHGMRGGEKAKDFKGTLGKLIKYLRPYY
                     FRLVIVVIFASASTVFAIVGPKILAKATDKLSEGIIAKVAGTGGIDFDYIGQIILILV
                     GLYLISALFSYIQGFITSTISQRVAYDLRTSISQKMDRMPLSYFDKHTSGDILSRVTN
                     DIDTIAQSLNQSMSQVITSTVTVIGIFIMMLTISPVMTLIAVCVLPVSMVLIGLVMKR
                     SQKYFARQQAALGDVNGHIEEMYGGHNVVKAFNGEAASVEQFNEYNDNLYESAWKSQF
                     FSGLMQPITGFVGNVGYVAVCLLGGVLAGGGSITIGDIQAFIQYVRQFNQPITQLAQT
                     MNMLQSTAAAAERVFEFLGEEELEPETPKVTADEVAKVEGSVTFADVNFGYLKDQTII
                     NDFSLHVHAGQTVAIVGPTGAGKTTIVKLLMRFYELNSGSILIDGKDIRDFGRQDLRS
                     LFGMVLQDTWLFNGTIKENLMYGKLDASDEEVKEACKVAYVDHFVQTLENGYETMINE
                     ESSNISQGQKQLLTIARAFLKDPKILILDEATSSVDTRTEVLIQKGMEKLMEGRTS"
BASE COUNT         1460 a          503 c          852 g         1373 t
ORIGIN      
        1 gaagttaaat attactaaag cgactttatc agtgagattg cagcgccttg aaaaactggg
       61 ttatttaact agaacacaag ataaaaatga taaacgtaat tatattttaa atattacaaa
      121 aactggagag gtatttattg aggcgacaat caaaattatg aaagaaaaaa caatgattat
      181 gtttgaaggg gtttctaagg aacagataac tgttattaat gatgttatta atatcatgaa
      241 aaagaatatt gaaaaatgta aaggagaaga ataatgctaa aattaaaaca ttattttaaa
      301 aagttttggg caccaattct actttgtgta ggattattat tcttacaatc acaatcagaa
      361 ttagcattac ctgattatat gtcggatatc gtttcggtag gaattcaggc tggtggcttt
      421 gatagtgcag taagtgatgt tttgagtgaa gaaacatata atcatttatt agtactgatg
      481 gatgaagagg atcagcaaca atttatggat gcatataaat tagttgaacc cagtaatctt
      541 gataaagata ctttagataa gtttcctaaa gcaaaaggtc aaaatatcta taaattaaaa
      601 gatctaagtg aaaaaaaatt agaccgttta gagagtattt tagttaaacc aatgttgatg
      661 gtaacatcaa ttgatggaat ggataaaaat agtaaagaat atcaagaaca gtttgggcaa
      721 ttaccgccaa atatgacacc ttacgatgct ttagcaatga tggataatac aactaaagct
      781 aaaatgttta gtaaaattga tagtcagatg gaaacgatgg gtgaatcaac tttaaaaatt
      841 gctgccggta acggtgtaaa agcagaatat tctagactag gttgtgatac tgataagatt
      901 caaaatgact atattttatg gtctgggtta aaaatgttag caattgcatt agctggtact
      961 gtatgtgctg ttgcttgtgg gttcttggct agtaaggttg gggctggtgt ttcgagatta
     1021 ttacgtcgtg atgtttttga gaaagttgaa agtttttcaa atgaagaatt caataagttt
     1081 tcaactgcat cactgattac aagaacaacg aatgatatta cccaagtaca aatggtcgta
     1141 attatgttca ttcgaatagt atgttttgct ccaatgatgg gaattggggc cttgatcaaa
     1201 gcttttaata atacaccatc aatgacttgg attattggtt tagttttatt ggtgattttt
     1261 tgtttgattg gtgttacttt tgcaattgtg atgccaaagt ttaagatcgt acaatcatta
     1321 attgaccgtt taaatttaac aatgcgggaa aatctttcag gtatgctagt aattagggca
     1381 tttggtaatg aaaagcatag tgaagaaaga tttgaaaagg ctaatggtga tctaacaaat
     1441 gtcaatttgt ttgtcaatcg aacaatggct tcattaatgc cgattatgat gttcattttt
     1501 aatgtagtaa cattattgat tgtatattat ggagcaaaac aaattgattt aggtaatatt
     1561 gcaatcggac aaatgatggc ttttatgcag tatgcaatgc aaattattat gtcatttttg
     1621 atgattgcaa tgatttcaat tatgttgcca cgggctagtg tagcagcaga tcgtttttat
     1681 gaagttattt caatggaacc taaaattgtt gatcctaagg aacctaaagc ttttattgaa
     1741 agtaaaaaag gtttagttga attcaataat gttacattta aatatcctgg tgctaatgaa
     1801 gcagtattag aaaatattag ttttactgca aagcctggtg aaactacagc atttatcggt
     1861 tcaacgggat ccggtaaaag tacgttaatc aatttaattc ctcgctttta tgaagtaact
     1921 gaaggtaata ttaagattga tggtgttgat attcgtgatg ttaatcaaca tgatttaaga
     1981 gataaaatcg gtttagtacc tcaaaaagga ttattattct caggaaccat tagatcaaat
     2041 ttaacttatg gtgcacctga ggctacagac gatgaacttg aagaagtaat tcgggtagct
     2101 caggcaaaag aatttattga taataaagaa gaaagattag atagtgaaat ttcacaaggt
     2161 ggaacaaatg tatctggcgg tcaaaaacaa cgtttagcaa ttgctcgagc tattgctaaa
     2221 aatccagaaa tctttatttt tgatgatagt ttttcagctc tagatttcaa gacagatgct
     2281 aaattacgtc aagaattaga taagatggtt aagaagacta aaaatacagt attgattgtc
     2341 ggacaacgta ttgcttcaat tatgggagct gaacaaatca ttgttttaga tgaaggtaag
     2401 attgttggaa aaggaaccca tgaagagtta atgaaaaatt gcgatgtcta tcaagaaatc
     2461 gcatattcac aattatcaaa ggaggaatta ggacatgagt aatcaaccaa aaagaaatgg
     2521 tcctaaattc ggacctggtg gtatgcacgg aatgcgtggc ggtgaaaaag ctaaagattt
     2581 taaaggtact ttaggtaaat taattaaata tttaagaccg tattattttc gtttagttat
     2641 agttgtgatt tttgcttcag catcaacggt ttttgcaatc gtaggaccaa agattttagc
     2701 taaagcaaca gataaattaa gtgaaggtat catagctaaa gtagctggta ctggtggaat
     2761 tgattttgat tatattggtc aaattatttt gattttagtt ggattgtatt tgattagtgc
     2821 tttatttagt tatatacaag gttttattac ttcaactatt tcacaacgag ttgcttatga
     2881 tttaagaaca tcaatttctc aaaagatgga tcggatgcca ttaagttatt ttgataaaca
     2941 tactagtggt gatattttaa gccgagtaac aaatgatatc gatacaatcg cacaatcact
     3001 aaatcaaagt atgtcacagg taattacgtc gacagtaacg gtaattggaa tctttatcat
     3061 gatgttgact atttcaccag ttatgacttt gatcgcagtt tgcgtattac cagtatctat
     3121 ggttttgatt ggtttagtaa tgaaacgttc acaaaaatat tttgcgcgtc aacaagctgc
     3181 tttaggtgat gtcaatggtc atattgaaga aatgtatggt ggtcataatg tggttaaagc
     3241 atttaatggt gaagctgctt cagtagaaca atttaatgag tataatgata atttatatga
     3301 aagtgcttgg aagtcacaat tcttttcggg tttgatgcag ccaatcacag gatttgttgg
     3361 aaatgttgga tacgttgcgg tatgtttatt aggtggtgtt cttgctggtg gtggtagtat
     3421 cacaattggt gatattcagg cctttattca atatgtaaga caatttaatc aaccaataac
     3481 acaattagca cagacaatga atatgttaca aagtacagca gcagcggctg aacgtgtttt
     3541 tgaattttta ggtgaagaag aattagaacc agaaacacca aaagttactg ctgatgaggt
     3601 ggctaaagtt gaaggttctg ttacctttgc tgatgttaat tttgggtatt taaaagatca
     3661 aacgattatt aatgatttta gtttacatgt tcatgcgggg caaactgttg caattgtagg
     3721 accgactggt gctggaaaaa caacgatcgt aaaattatta atgcgattct atgaattaaa
     3781 tagtggttcg attcttattg atggtaaaga tattcgtgat tttggacgtc aggatttacg
     3841 atcattattt ggaatggttt tacaagatac ttggttgttt aatggaacaa ttaaggaaaa
     3901 cctaatgtat ggaaaattag atgcaagtga tgaagaagtc aaggaggctt gtaaagtcgc
     3961 ttatgttgat cattttgtac aaacattaga gaatggatat gaaacaatga tcaatgagga
     4021 atcatctaac atttctcaag ggcaaaaaca acttttaaca attgcacgtg ctttcttaaa
     4081 agatccaaaa attttgattc ttgatgaagc aacttcttca gtagatacaa gaacagaagt
     4141 attaattcaa aaaggaatgg aaaagctaat ggagggaaga acaagttt
//
LOCUS       DAOMTA010000186         4186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_653_length_4186_cov_2.726701, whole genome shotgun sequence.
ACCESSION   DAOMTA010000186 DAOMTA010000000
VERSION     DAOMTA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_4186_cov_2.726701"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLK68_06295"
     CDS             complement(<1..693)
                     /locus_tag="PLK68_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM90055.1"
                     /translation="MVDFNNLMDLQFEIFILMIIGYILRKTNIISKEHRKSLTDLVIY
                     IVLPANIIYSFMIKMDTQIIKSGLTILIVSIIIQFACQVFGKYFFIKATKRQQSVLQY
                     GTICSNAGFMGSPLIQGLYGLDGLLFASIYLIPQRIVMWSGGVACFTNAKGKDVIKKV
                     ITHPCIIAVFIGLFIMISQIQLPSFLKVSIQSLSNCTMALSMIVIGGILAEIKIRDVI
                     NRLTLYYSFIRLI"
     gene            806..1051
                     /locus_tag="PLK68_06300"
     CDS             806..1051
                     /locus_tag="PLK68_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM90056.1"
                     /translation="MKTNYVYIVKCQDNTFYTGWTTDLVKRINAHNHGKGAKYTKARR
                     PVELVYFEEYTEKSLALKREYAIKQMTRKEKELLINS"
     gene            1126..1998
                     /locus_tag="PLK68_06305"
     CDS             1126..1998
                     /locus_tag="PLK68_06305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HRM90057.1"
                     /translation="MKLNRNDLCWCGSQKKYKKCHLAFDEKLEQLKQQGKKIPTHKMI
                     KTPEQIEGIKKAAVINSGLLDYIEANIKVGMTTEEIDVIAKEYTAKYGAVCADYQYMG
                     YPKHICVSINDVVCHGIPNEQTILQEGDIVNVDATTMLNGYYADASRMFMIGKVSNEA
                     KKLVSDTKKALELGMASVIPYQSCVGDIGKAIETFAKAMGYSVVREFCGHGVGLAIHE
                     DPYVFHFEPNVPTVTLVPGMVFTIEPMLNLGGREVYVDESDEWTVYTDDESLSAQWEH
                     TLLVTEDGIEIISK"
     gene            complement(2080..3162)
                     /locus_tag="PLK68_06310"
     CDS             complement(2080..3162)
                     /locus_tag="PLK68_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM90058.1"
                     /translation="MYLYMGLLAVIPGIIYFTFFINRLLRTILNIHTNWFLIIIIGLV
                     IMVLSIPAMNTLELYGVIYYHLLVVMLLFELLNLGLKRFPIYRISFTTGILGIVVTAL
                     FLGYGYYNIKHVVATTYDLKSDKVEDLKILEIADLHMSTSLSVTELQKYCDEMSQLNA
                     DLVVLTGDIFDENTPLDDMVNASKALASINNQQGIYYVYGNHDNGSHAFSDSEFGPED
                     VRATLEKNGIVVLEDAVVSLDNINIIGRKDASFWGTNPRLSTSQLLEMIPENKRGNYT
                     IMLDHQPLNLDENAALGIDLQLSGHTHGGQLFPMGIVQSLTSDTLIRGQRDIGDFTAI
                     TTTGIAGWRYPIKTGAPSEYVIINIK"
     gene            3519..4172
                     /locus_tag="PLK68_06315"
     CDS             3519..4172
                     /locus_tag="PLK68_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HRM90059.1"
                     /translation="MKKRFLIFLLIIAPLCINAVHASEDNAKLQLLKEKHYETEGTIV
                     TVKIPHVVNVKDDKVKKVINKLITQAIDDFTNEFKEFDKEPNTEHKLIADITFQNYYS
                     DDKIISFSINATQIMADSYLQKKFYTVDLKTGEVYNIEHFLGSDYQNIVKKSVQQQIA
                     ENKEKYPNLMYFDEAVNNLKITNEQPFYINKDNQVVVVFNQFEIAPGYMSLPEFIIK"
BASE COUNT         1560 a          702 c          621 g         1303 t
ORIGIN      
        1 aatcaaacgg ataaaactat agtagagggt caatctattt ataacatctc taattttgat
       61 ttctgccaag attccaccta ttacaatcat tgataaagcc atcgtacaat tactaagtga
      121 ctggattgat acttttaaaa aacttggtaa ttgaatttgg ctaatcataa taaacaggcc
      181 aataaatact gcgataatgc aaggatgtgt aattactttc ttaataacat ctttaccttt
      241 agcattagta aaacatgcaa ccccaccaga ccacattaca attctttggg gaattaaata
      301 aatggaagca aataataatc catctaatcc ataaaggcct tgaatcaagg gtgagcccat
      361 aaatccagca ttggagcaga tcgttccgta ttgtaaaaca ctttgttgtc gttttgttgc
      421 cttaataaag aaatactttc caaatacttg gcaagcaaac tgaataataa tcgaaacaat
      481 caaaattgtt agaccactct tgatgatttg agtatccatt ttaatcataa aagagtaaat
      541 aatattagcc ggcagtacaa tatatattac taaatccgtc aagctctttc gatgctcttt
      601 agaaataata tttgtctttc ttaaaatata tccaattatc attaaaataa atatttcaaa
      661 ttgtaaatcc attaaattat taaaatcaac cacaataaca cctctttgaa gatatcttaa
      721 actaattttc ttgacacgtc atctaaaact taccattatt catataatta gttataatat
      781 aaacaataaa agtaagggtg aaataatgaa aacaaactac gtctatatcg tcaaatgtca
      841 ggataatact ttctatacag gctggacaac tgatctagta aagcgaatca atgctcataa
      901 tcatggcaaa ggtgcaaaat ataccaaagc tcgtcgcccc gttgaactgg tttactttga
      961 ggagtatact gaaaaaagcc ttgcgttgaa acgggaatac gctattaagc aaatgacacg
     1021 taaagaaaaa gaattattaa tcaacagtta gcataggcta agtaatttct ttggaaatta
     1081 aagaataacg tgttatacta gattcatata gaaagaaggc aaaatatgaa attaaaccga
     1141 aatgatttat gctggtgcgg cagtcaaaaa aaatacaaaa aatgccatct agcatttgat
     1201 gaaaaattag agcaattaaa gcagcaagga aaaaaaattc caacccataa aatgataaag
     1261 acgcctgagc aaattgaggg aattaaaaaa gccgcagtaa taaactcagg tttattagac
     1321 tatattgaag caaatatcaa agttggaatg acaactgaag aaattgatgt tatagcaaag
     1381 gaatatactg ccaaatatgg ggcagtttgt gctgattatc aatatatggg ttatccaaaa
     1441 catatttgtg tctcgatcaa cgatgttgtt tgtcatggta ttcctaatga gcaaactatt
     1501 ttacaagaag gtgatattgt aaatgttgat gctacaacca tgctgaatgg ttattatgcc
     1561 gacgcttcta gaatgtttat gattggtaaa gtttctaatg aagctaaaaa actagtcagc
     1621 gatacaaaaa aggctttaga actcggtatg gctagtgtta tcccctatca aagttgtgtt
     1681 ggcgacattg gtaaagcaat cgaaacattt gctaaagcta tgggttatag tgttgttaga
     1741 gaattttgtg gtcatggtgt tggtttagcg attcatgaag atccttatgt tttccatttt
     1801 gagccaaatg tgcctactgt tacccttgtc ccaggaatgg tctttacaat tgaaccaatg
     1861 ttaaatcttg gtggtcgtga agtatacgtt gatgagtcag atgaatggac tgtttatact
     1921 gatgatgaat cactttcagc acaatgggag catacgttgt tagtgactga agatggtatt
     1981 gaaattattt caaaataata aaacctccga tttgaagtga accaccatta tttagattag
     2041 acacctaaat aatgggattc atttcagttt ggaggtcttt tattttatat ttataattac
     2101 atactcacta ggagcacctg ttttgattgg ataacgccat cccgctattc ctgtcgtcgt
     2161 aattgctgta aaatcaccaa tatccctttg tccacgaatc aacgtatcac ttgtcaatga
     2221 ctgaacaatt cccataggga acaattgtcc accatgagta tgcccagata attgtaaatc
     2281 aattcctaga gctgcatttt catcaaggtt taaaggctga tgatcgagca tgatcgtgta
     2341 gttaccacgt ttattttcag gaatcatctc taataattga ctagttgaca aacgaggatt
     2401 tgtaccccaa aaacttgcat cttttcgacc aataatattt atattgtcta agctaactac
     2461 tgcatcttcc aatacaacga ttccattttt ctccaacgta gcacgaacat cttcaggacc
     2521 aaattcacta tcgctaaaag catgagagcc attgtcatga ttaccataaa catagtagat
     2581 tccttgttga ttattgatcg atgctagcgc cttactagca ttaaccatat catctaatgg
     2641 ggtattttca tcaaagatat cccctgttaa aactactaaa tcagcattca gctggctcat
     2701 ttcatcacaa tatttttgta gctccgtaac acttaatgat gttgacatat gtaagtcagc
     2761 aatttcaaga attttaagat cctcaacttt atcacttttt agatcatatg ttgtagctac
     2821 aacatgttta atattatagt agccataacc taaaaataac gccgtaacta ctattcccaa
     2881 aataccagta gtaaatgaaa tccgataaat cgggaatctt tttaagccta gatttaatag
     2941 ctcaaacaac aacatgacaa ccaataaatg ataataaata accccatata actccaacgt
     3001 gttcatcgct ggaattgata gcaccattat cactaatcca attataataa tgaggaacca
     3061 attagtatga atatttaaaa tcgttcgtaa taaacggtta ataaaaaaag taaaataaat
     3121 aataccgggt ataaccgcaa gcaaacccat atataagtac ataaaatccc tccttttaat
     3181 ttagaataca cgctgaagta tgcttcaggt caagtaattt ttataaaaca catatcttca
     3241 catatttata ttctaacaaa ataaaaagag catataacaa cataatttac taaacaaaca
     3301 acaaaaatat tccaaacagt cttaaacatt accagcttta tcctactctc tatttttacc
     3361 gtactctctt tttattaccc aacatattac taaaaataat taataaatca tatttcctta
     3421 acaagaaaat tttagtttga ataacttatc aaattatcgt aaaaactata atgatagcaa
     3481 agctatcaac acatttatca actagaaaga gatgaaatat gaagaaacgt ttcctgattt
     3541 ttttactaat tattgcacca ttatgtatca atgctgttca tgccagtgaa gataatgcta
     3601 aacttcaatt attaaaagag aaacattatg aaactgaagg tacaatcgtt actgtcaaga
     3661 ttccccatgt tgttaatgtt aaagatgaca aagtaaaaaa agttattaat aagctgatta
     3721 cccaggcaat tgatgacttt actaacgaat ttaaagaatt tgacaaagaa ccaaacacag
     3781 aacataaatt aattgcagat attactttcc aaaactacta tagtgacgat aaaataatat
     3841 cattttcaat taatgccacc caaatcatgg cagacagcta tttacaaaag aaattttata
     3901 cagtagatct taaaactggc gaagtctaca atattgaaca tttcttaggt tcagattatc
     3961 aaaatatcgt taaaaaatct gtacaacaac agattgcgga gaataaagaa aaatatccta
     4021 acctaatgta ttttgatgaa gcggttaata atttaaagat tactaatgaa caaccatttt
     4081 atatcaacaa agataatcag gtagttgttg ttttcaatca atttgaaata gctcctggtt
     4141 atatgtcatt accggaattt attattaaat agttgataaa tctaga
//
LOCUS       DAOMTA010000187         4182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_655_length_4182_cov_2.124061, whole genome shotgun sequence.
ACCESSION   DAOMTA010000187 DAOMTA010000000
VERSION     DAOMTA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_655_length_4182_cov_2.124061"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..296)
                     /locus_tag="PLK68_06320"
     CDS             complement(123..296)
                     /locus_tag="PLK68_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRM90060.1"
                     /translation="MTQSMLRVHCCIDTGPTEGFWGIMKCEMYHYGKYYNTKDELVKA
                     VDDWIHYYMYERN"
     gene            complement(475..720)
                     /locus_tag="PLK68_06325"
     CDS             complement(475..720)
                     /locus_tag="PLK68_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM90061.1"
                     /translation="MIKYCLEHDMDYKITAKLFETTYANVFNWVKKYKEKGEDGLGDK
                     RGCRKDDEKVDEVTLLKRQLKQKEYELEMVQLELKLS"
     gene            complement(923..1126)
                     /locus_tag="PLK68_06330"
     CDS             complement(923..1126)
                     /locus_tag="PLK68_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM90062.1"
                     /translation="MGRKNKYLKELKLSIVKRYLEGEGSTISLAKEINTSGNIVGNWV
                     KNIMHLVKVHLIFLIQMHLIQRI"
     gene            complement(1219..2139)
                     /locus_tag="PLK68_06335"
     CDS             complement(1219..2139)
                     /locus_tag="PLK68_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HRM90063.1"
                     /translation="MLLQVSGRTDICALYPKWFVNRLKAGYILVRNPYNEHQVSHVDV
                     TPEVVDCICFCTKDPKAIVPYLTQIDSMGYNYYFMVTITAYDLDIEPGLRPKLEIMKT
                     FIELSKMLGKKRVIWRYHPVLLNQRYTKVFHYKMFEKMCQLLFPYTETVIISFLDIYK
                     NIIGKFDELTDHDVIELAQRFGKIAQKYQLHIQTCSEKYHLEEYGIAKGSCLDRGYIE
                     TLIGYPLDIKTNTNRANCSCLASIDIGAYSCCNHGCSYCYACNHNLVAKNMAIHDDES
                     PMLLGTLSYDDKVNKRIVASNKVRQIKLDI"
     gene            complement(2142..2360)
                     /locus_tag="PLK68_06340"
     CDS             complement(2142..2360)
                     /locus_tag="PLK68_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90064.1"
                     /translation="MFKKYYYNNSALIIYIIEEKVKFYAIVINGFYKYFKEMFQRFVI
                     KVKKKQQKNLVKKTANNQIIRLLLIKGE"
     gene            2471..3220
                     /locus_tag="PLK68_06345"
     CDS             2471..3220
                     /locus_tag="PLK68_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM90065.1"
                     /translation="MNEQKLINLIGEIGRLLLKHGAEIYRVEESLLRMCKSYGYLNVE
                     VFALPSYFTLTIEMHDNHTYTLSKRTQQNRVNLDMVYELNNLVRYTCQECPDYIHLKS
                     KLEKIKNTPINMPLVFLGYGLGTGFFTTFFGGGIHETIIAVGIGFILYFFIWLMEILE
                     INNLVSTLLSSMVLTAIAIISLKLGLITNLQSTIIGCLMILTPGVAITNSLRDIMGSD
                     HISGMARMLEALLTATFIAIGVGTMMMVLGG"
     gene            3222..3677
                     /locus_tag="PLK68_06350"
     CDS             3222..3677
                     /locus_tag="PLK68_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM90066.1"
                     /translation="MQSIIECFSAFIACLGFAFIFRIHQHPRFAIIGSIGGALGWLVY
                     LLASNFKSEISCYFLAMAFVALFSEISARIYKAPATIFLIIGCFPLVPGRGIYQTMLY
                     CTQGNSELFLDSFLNTLAISASLALAILIISTIFKVIKKLSCRKIKVNS"
     gene            3674..>4182
                     /locus_tag="PLK68_06355"
     CDS             3674..>4182
                     /locus_tag="PLK68_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM90067.1"
                     /translation="MKVLGLIVEYNPFHQGHLYHINKAKQLIKPDVTIVIMSGHFVQR
                     GEPAISNKWTRAGVAIKNGIDLVIELPFVYSVQSADYFAQGAIELLAKLKVTDIVFGS
                     ECGNINIFKDIAFTIKNNQKNYDNLVKKQMNQGLRYPDACNQALSILMNKTVTTPNDL
                     LGLAYVKEV"
BASE COUNT         1333 a          706 c          596 g         1547 t
ORIGIN      
        1 gtatagtgtt ctattttata ttttattatt cttttatttt caggtactgg atattgattt
       61 ggtgtttgta aagagaatgc ctcatttcta acttcatatg gtgtcttaac tccaaatctt
      121 ctctaatttc tttcatacat gtaataatga atccaatcat caacagcttt gacaagttca
      181 tcttttgtat tgtaatattt tccgtaatga tacatctcac atttcatgat tccccaaaac
      241 ccttccgttg gtccagtatc tatgcaacag tgaaccctta acatactttg tgtcatacca
      301 tgtcccttaa gcatgtttat aaacactgaa cttgtatatt aaaaacctct accactgtgg
      361 aataaaggac gtgcattagg attatcttta accgccttat caaatgtgtt aaagacaagt
      421 atgttattat tgtgatcacc aacttcataa ccgacaacta ttctatcata aagatcaaga
      481 aagcttaagt tccagttgta ccatttcaag ttcgtattcc ttctgcttga gctgacgttt
      541 taataatgta acttcatcaa ctttttcatc atccttacga cagccacgct tatcacctaa
      601 accatcttct cctttttcct tatacttctt aacccagtta aaaacattag cgtaagttgt
      661 ttcaaacagc ttagctgtta tcttataatc catatcatgt tcaagacagt actttataat
      721 ttcaatacgt tcttccttat tgactttctg cttttcacca tatagatatc ttctcctcca
      781 ggaatataat cttttagttc tatatgacta ttatacaatt ttacccaatt ttttatagga
      841 ctagatgacg gaatacaata ttttattgcc aaatcatcat atgatccccc accgttaaga
      901 taatctaata cgacctattt ttttaaattc tttgtataag atgcatttgt attagaaata
      961 tcaaatgcac tttcaccaaa tgcattatat tttttaccca gttgcccact atattgcctg
     1021 atgtatttat ttctttggct aaggagatgg ttgatccttc accctctaaa tatcttttga
     1081 caatacttaa ttttagttct tttaaatatt tattttttct tcccaatata aaaacctcct
     1141 gtatcaatgt ttaattattt acactgtcta ctgggaggtt atcatatcat ctaattaaat
     1201 tagtctttat tttactagtt atatatctaa tttaatttgg cgaactttat tcgaagcaac
     1261 aattctttta ttaactttat catcatatga taaagttcct aacagcatcg gactttcatc
     1321 atcatgaatt gccatatttt ttgcaactaa attatgatta catgcataac aataagaaca
     1381 tccatgatta caacagctat atgcaccaat atcaatactc gctaaacaac tacaattggc
     1441 acgattagta tttgtcttga tatccaaggg atatcctatc aacgtttcaa tatatcctcg
     1501 atctaaacaa ctacctttag caattccata ttcctctaaa tgatatttct cactacaagt
     1561 ttgaatatga agttgatatt tttgagcaat ttttccaaac cgctgggcca attctattac
     1621 atcatgatca gttaactcat caaacttacc aataatattt ttgtaaatat caagaaaact
     1681 aataattacg gtttctgtat acggaaagag cagttggcac attttttcaa acattttata
     1741 atgaaatact tttgtataac gttgatttaa cagaactgga tgatagcgcc agatcacccg
     1801 cttttttcca agcatcttac ttaattcaat aaacgtcttc attatttcta gttttggtct
     1861 taatcccggt tcaatatcta aatcataggc agtaatagta accataaaat agtaattgta
     1921 tcccatgctg tctatttggg tcaaataggg gactattgct ttgggatctt tagtacaaaa
     1981 gcagatgcaa tcaacaactt ctggagtaac atctacgtgt gagacctgat gctcattata
     2041 aggatttcga actaatatgt atcccgcttt taatcgatta acaaaccatt taggataaag
     2101 tgcacatata tcagttcgtc cagagacttg aagtaacatt attattctcc ctttatcaat
     2161 aacagcctta ttatttggtt atttgctgtt tttttaacta aatttttctg ttgtttcttt
     2221 ttaactttaa ttacaaatcg ttgaaacatt tctttaaaat atttataaaa accatttatg
     2281 acaattgcat aaaattttac tttttcttca ataatataaa tgatcaaagc tgaattgtta
     2341 taataatatt ttttaaacac tttctcacct acctcttagc tattttatca taatttatta
     2401 caaatattaa caatcatgat atatcattaa acaattattc atggtatact tttgattatc
     2461 gaggtgggtt atgaacgagc aaaaattaat taatttaatc ggtgaaattg ggcggctttt
     2521 attaaaacat ggggctgaaa tttaccgggt tgaagaatca ttattgagaa tgtgtaaaag
     2581 ttatggttat ttaaatgttg aagtatttgc tttaccttca tactttactc taacgattga
     2641 aatgcatgat aatcatacat atactttatc aaaacggact caacaaaatc gtgttaattt
     2701 agatatggtc tatgaattaa ataatctagt ccgctatact tgtcaagaat gtcccgacta
     2761 tattcattta aaaagtaaac ttgaaaaaat aaaaaacaca ccaatcaaca tgcccttagt
     2821 atttctaggt tatgggttag gaactgggtt ttttacaact ttttttggtg gtggaatcca
     2881 cgaaacaatt attgctgtag gaattggatt tatcttatat ttctttattt ggttaatgga
     2941 aatattagaa ataaataatc ttgtcagtac attgttatca agcatggttt taactgccat
     3001 agcaataatt tctttaaaat taggtttaat aaccaattta cagtctacta tcattggctg
     3061 tttaatgatt ttaactcctg gggttgctat taccaatagt ttaagagata ttatgggtag
     3121 tgatcatatt tcaggaatgg cacgaatgtt agaggcttta ttaacagcca cttttattgc
     3181 aattggggtt ggaacaatga tgatggtctt aggagggtaa tatgcaaagt ataattgaat
     3241 gttttagtgc tttcattgca tgtctcgggt ttgcttttat ttttcgtatt catcagcatc
     3301 cacgctttgc tataatcggg tcaatcggcg gggcattagg ctggttagtc tatttgcttg
     3361 cttcaaattt taaaagtgaa atcagctgtt attttttagc aatggcattt gttgctttat
     3421 tttctgagat atccgcccga atctataaag ctcctgccac aattttttta attatcggct
     3481 gtttccccct ggttcctgga cgtggaatct atcaaacaat gctatactgt acgcaaggta
     3541 acagtgaatt atttttagat agctttttaa atacactagc gatttctgct agtcttgctt
     3601 tagcaatctt gattatttca actattttca aagtaatcaa aaaattatca tgtcgtaaaa
     3661 taaaggtgaa ctcatgaaag tactaggtct aattgtcgaa tacaatcctt ttcatcaagg
     3721 acatttatat catatcaata aagcaaaaca actaattaaa ccagatgtta cgatcgtaat
     3781 aatgtctgga cattttgttc aacgcggtga gccagcaatt agtaacaagt ggacaagagc
     3841 aggagttgca attaaaaatg gcattgactt agtaattgag ctgccatttg tttatagtgt
     3901 tcaaagtgct gattattttg cccaaggagc aattgaatta ttagcaaagc taaaagtcac
     3961 tgatattgtt tttggaagcg aatgtggaaa tatcaatatt ttcaaagata ttgccttcac
     4021 gattaaaaat aatcaaaaga attatgataa cttagtaaaa aaacagatga atcagggttt
     4081 gcgttatcca gatgcctgta atcaggcact atctatttta atgaataaaa ctgttactac
     4141 ccctaatgac ttattagggc ttgcctatgt taaagaagta at
//
LOCUS       DAOMTA010000188         4164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_662_length_4164_cov_2.819177, whole genome shotgun sequence.
ACCESSION   DAOMTA010000188 DAOMTA010000000
VERSION     DAOMTA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_4164_cov_2.819177"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLK68_06360"
     CDS             <1..287
                     /locus_tag="PLK68_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HRM90068.1"
                     /translation="KGYNYLVTAIVLVCGNINLLGEITKELYPRIARTYGTTASRVEQ
                     SIRHVLKCTWESGCQDELEQLFGFRAKRKTCNSEFISTIVDELLSKYKGS"
     gene            complement(399..1214)
                     /gene="spo0A"
                     /locus_tag="PLK68_06365"
     CDS             complement(399..1214)
                     /gene="spo0A"
                     /locus_tag="PLK68_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRM90069.1"
                     /translation="MTDVKKYTVYILEENKDLLNNIKDSLIATNNFTVIGTSDNTTSC
                     LNYLSNNNCDLLIIDLMLTNIDGIGVLNKLKEVNSRAYNKVVCITSFTNPLICETLEK
                     LNVSYCFKQPFDINYFATTLNSVMKVTLEDIKGMSQNESEKYQKIKLENEITDILHEV
                     GIPAHIKGYMYLRTAILSTYYNIELLGQVTKVLYPDIARQYSTTASRVERAIRHAIEV
                     AWNRGNTDAIDDIFGYTVSATKSKPTNSEFIAMIADKLRLEHQTKAASRHSYL"
     gene            complement(1412..2515)
                     /locus_tag="PLK68_06370"
     CDS             complement(1412..2515)
                     /locus_tag="PLK68_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRM90070.1"
                     /translation="MKKILLTILSLFLLTGCFSFREKSNSRPAITIDTDYLSGDASYL
                     YYYQSLSSKEKEIYENIYNCILDNAKKVTISSNDYELVQKINDYVLYDHPEIYYLDYF
                     ELQNQVDICNYIPSYSYSKSERDTLTAQLESVRDELVNSISSESSDYDKLKKIYQFVI
                     EKCRYVDNAKDNQYITSSLIYGETVCSGYVKAIQYLAEAVGIKSAYIVGKEIGASDDE
                     AYHAWNLIYLDDDYYYLDATWGDYDSEGNIFAMMNYFMFDSDDMLKLYEPLDQYEITK
                     QGNYTYFKYENLYNENYNKAALNKMVKQYKHDNIAWMEFKFSDSCYQEAKKRLIDQEE
                     MFDLFNPYTSKQYTVQYFYYDNLNVLIFNQKIE"
     gene            2759..>4164
                     /locus_tag="PLK68_06375"
     CDS             2759..>4164
                     /locus_tag="PLK68_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM90071.1"
                     /translation="MKDNKFIKFITSKYLILAVQLFATALFTYLIFQLDLVPLKYLIP
                     ATGALGLLIIIFFFIMRSGQKKINQGLKSKRSIVTKIISLLMSILLMFASSYVVRGND
                     FFNTVTKATTQKYLVSVITMKNNSATKLSDLDGKKFGVSYQHDTTTITKAIADMENDL
                     GEQEDMVKYDDYSGLADALYKGEVDAIIVGQEYKSMLEANHDSFDDETKIIKSYEYES
                     KLSVTTKQTNVTENPFTIYVTGIDTYGSVSTVARSDVNLIVTVNPKTKQILMTSIPRD
                     CEIQLHKNGKMDKLTHTGIYGTSETISTIEDFLDVEINYFARTNFSGMTNIVDALGGV
                     TIDSDYKFTTLHGNYNIVKGENQMDGDKALCFVRERYSLPNGDFDRGKNQQKLLKAML
                     EKAMSPKIITNFNNILTAIEGSFETDMSSKEIKSLLNMQLNDMSDWTVYNVQVEGEGY
                     KTSKTASMYGTEVYVMKP"
BASE COUNT         1479 a          563 c          660 g         1462 t
ORIGIN      
        1 taaaaggcta taattatcta gttacagcaa ttgttttagt ttgtggaaat attaatcttt
       61 taggagaaat tactaaagaa ttatatccgc gtattgctag aacttatgga acaacggcat
      121 cacgagttga acagtcgatt cgtcatgttt taaaatgtac ttgggaaagt ggttgtcaag
      181 atgagctaga gcagctattt ggatttcggg caaaacgtaa aacatgtaat tctgaattta
      241 tttcaacaat tgtagatgag ttattatcaa agtataaagg aagttaacga tccgaggatt
      301 taattcctct ttttatgtca gttatggaga tctccatgat tgatataaga aaaagattag
      361 tttttctaaa attaggtaga aaaaaagagc ttacgctctt ataagtaact atgacggctt
      421 gccgcttttg tttgatgttc tagacgaagt ttatcagcaa tcattgcaat aaattcagaa
      481 ttggtaggtt ttgatttagt tgcactgact gtataaccaa agatatcatc aattgcatca
      541 gtattaccgc ggttccaagc aacttcaata gcatgacgaa tggcacgttc tactcttgaa
      601 gcggtagtac tgtattggcg ggcaatatct ggataaagga ctttagttac ttgacctaat
      661 aattcgatat tatagtaagt agatagaatt gcagttctta ggtacatata tcctttgatg
      721 tgggctggaa tcccaacttc atgaaggata tctgtaattt cattttctaa cttaattttt
      781 tgatattttt cagattcgtt ttgtgacata cctttaatat cttctaatgt aactttcatt
      841 acagaattta aggttgtcgc aaaataattg atatcgaatg gttgtttaaa acaataactt
      901 acatttaatt tttctaatgt ttcacaaatt aatggattag taaagctagt aatacaaact
      961 actttattat aggcacgact atttacttcc ttaagtttat ttagaacacc gattccatca
     1021 atgttggtaa gcattaaatc aataattaat aaatcacaat tattattaga taaatagttt
     1081 aaacagcttg ttgtattatc gctagtacca ataacggtaa agttatttgt cgcaattaaa
     1141 ctgtccttaa tattattcaa taaatccttg ttttcttcta aaatgtatac tgtatacttt
     1201 ttaacgtctg tcatctctat attccccttt cgttttagtg acaaaattta ttatatttat
     1261 acagtataaa agagaaaagg gtttatttat agataattag ggatttagta aagtaaggaa
     1321 ttaagttccc tataacaaga aagttttcgt gaaaattcgt gaagaattct cgaattttca
     1381 aggataataa cgagtcgtgt cgaacggctg attattcgat tttttgatta aaaattaaaa
     1441 catttaaatt atcataataa aaatattgaa cagtatattg cttacttgta tagggattga
     1501 aaaggtcaaa catttcctct tgatcaatca atcttttttt tgcttcctga taacaactgt
     1561 cactaaattt aaattccatc caggcaatat tatcatgttt atactgtttt accattttat
     1621 ttaatgctgc tttattgtaa ttttcgttat aaagattctc atatttgaaa tacgtataat
     1681 ttccttgttt cgttatttca tattgatcaa gaggttcata taatttcaac atatcatcag
     1741 aatcaaacat aaaatagttc atcattgcaa aaatattgcc ttcgctatca taatcacccc
     1801 aagtagcatc aagatagtaa tagtcatcat ctagataaat taaattccag gcatgataag
     1861 cttcatcgtc actagcacca atttctttac ccacgatata ggcgcttttt atcccaactg
     1921 cttcagcaag atactgaata gcctttacat agccgctgca aactgtttca ccataaatta
     1981 acgagctagt aatatattga ttatctttag cattatcaac atatcgacac ttctcaatta
     2041 caaattgata aatttttttt aatttatcat aatcactact ttcacttgaa atcgaattta
     2101 ctaattcatc acggacagat tctaattgag ctgttaaagt gtcacgttca cttttagaat
     2161 atgaataact gggaatataa ttacatatat caacttgatt ttgtagctca aaataatcta
     2221 gataatagat ttcgggatga tcatataaaa cataatcatt tattttttga actaattcat
     2281 aatcattact gctgattgtg accttttttg cattatcaag aatgcaatta taaatatttt
     2341 cataaatttc tttttcttta gatgataaag actgataata gtataaataa ctcgcatcgc
     2401 cgcttaagta gtcagtatca atagttattg ctggtcgaga attagacttt tcacgaaaac
     2461 taaaacaccc ggttaataaa aatagtgata atatcgtcag taatatcttt ttcatttttg
     2521 gattcctcct atttttagta taacatggat tattcatgag aaagaaggat tatgataaaa
     2581 gtggctaaaa ttatcacata aagaccataa tataacatgt ttattaataa acatagatga
     2641 atgattaata tttcttacaa aattcttaaa atttctattt atttcgacat aaatttgtat
     2701 tataaaacag attatgatat tatttaacgg taaaaaagtg cgaatatagg aggcttatat
     2761 gaaagacaat aagtttatta aatttattac ttctaagtat ttgattttgg cagtgcagtt
     2821 attcgcaacc gcattgttta catatttaat ctttcaattg gatctagtac cattaaaata
     2881 cttgattcca gcgacagggg cattaggttt attaattata attttcttct ttatcatgag
     2941 gagcgggcaa aagaaaatta atcaaggttt aaaaagcaag cgttcaatcg ttacaaaaat
     3001 tattagctta ttaatgagta ttttattaat gttcgcatca agttatgttg ttaggggaaa
     3061 tgattttttt aataccgtta ctaaagcaac aacacaaaaa tatttagttt cagtgattac
     3121 aatgaaaaat aatagtgcaa ctaaattaag tgatcttgat ggaaagaaat ttggtgtatc
     3181 ttatcaacat gatacaacga cgattactaa agcaattgct gatatggaaa atgatcttgg
     3241 ggaacaagaa gatatggtta aatatgatga ctatagcgga ttggccgatg ctctgtataa
     3301 aggtgaagta gatgctatta ttgttggaca agaatataag tcgatgttgg aagcaaatca
     3361 tgatagtttt gatgacgaaa caaaaattat taaatcatat gaatatgaat ctaagttaag
     3421 tgttacaact aaacaaacaa atgtaacaga aaacccattt acaatttatg ttactggaat
     3481 tgatacatat ggtagtgtta gtaccgtagc tcgaagtgat gtaaacttga ttgttactgt
     3541 taatcctaaa acaaaacaaa ttttgatgac cagtattccg cgtgattgtg aaattcaatt
     3601 gcataagaat ggtaaaatgg ataaattaac tcataccgga atctatggaa caagtgaaac
     3661 aattagtacg attgaagatt tcttagatgt tgaaattaat tattttgcga gaactaattt
     3721 tagtggaatg acaaatattg tagatgctct tggtggggta acaatcgatt ctgattataa
     3781 attcacaaca ttacatggta attacaacat tgttaaaggc gaaaaccaaa tggatgggga
     3841 taaagcatta tgctttgttc gggaaagata ttctttacca aatggagatt ttgaccgtgg
     3901 taaaaatcaa caaaaattat taaaagcaat gttagaaaaa gcaatgtcac caaagataat
     3961 cactaatttt aataatattt tgactgcgat tgaaggaagt tttgaaactg atatgtcaag
     4021 taaagaaatt aagtcgttat tgaatatgca gttaaacgat atgtcggatt ggactgttta
     4081 taatgttcaa gtagaaggtg aaggttataa aacttcgaaa actgcatcaa tgtatgggac
     4141 agaggtctat gtaatgaaac ctta
//
LOCUS       DAOMTA010000189         4133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_672_length_4133_cov_2.396027, whole genome shotgun sequence.
ACCESSION   DAOMTA010000189 DAOMTA010000000
VERSION     DAOMTA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_4133_cov_2.396027"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..343)
                     /locus_tag="PLK68_06380"
     CDS             complement(14..343)
                     /locus_tag="PLK68_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90072.1"
                     /translation="MTKRFEFDDDLEELDIDEPEQDEAVEETGNKKQRRPKTKKKKVT
                     KTKRKTKTKTKVKKEKQGRSKFSTFILSLLILLIITGACVGGYFAYEYYIDQQKQIDN
                     LQLVSNR"
     gene            complement(411..1133)
                     /locus_tag="PLK68_06385"
     CDS             complement(411..1133)
                     /locus_tag="PLK68_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM90073.1"
                     /translation="MKQINKMKVLVVTLLMIGLVMSLFILVNDQVNQSNNQLIAANKV
                     KTNQNKSANSVVKDELLTLVNFENTIPKDWKVDLVQLNNGQSVDRRIYDDLIAMLQAA
                     KSEGLNPLICSSYRTNEKQEQLYQNKVSEYLSQGYSKVEASDKAAFWVARPGTSEHQL
                     GLAVDIVSTKNQRLDRSQENTVEQQWLIQNSWKYGFVLRYPTNKNSITGVGYEPWHYR
                     YVGKEHAKKINELGVCLEEYVK"
     gene            complement(1232..1894)
                     /locus_tag="PLK68_06390"
     CDS             complement(1232..1894)
                     /locus_tag="PLK68_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90074.1"
                     /translation="MRYSKNIIKFAAAIILLIIIPLVPLTVSKIQDDKLIEHLQVEKI
                     KSDNNEIQTSKLTVAEKLELIGDYENKEKNIITTTQVQDMSDENITRIRTIINEQLVI
                     LKNLGILTDFNFDGNYVCYNYTLRRYSNVIDSSKSVSVYQVNFTNEEGIFNATIDVDT
                     HLIYQYNYYNKKYIARNYEVIYTFGTAYLGLTEQETYKYLFGIIDNRTDSVSVSSYND
                     IY"
     gene            complement(1884..3323)
                     /locus_tag="PLK68_06395"
     CDS             complement(1884..3323)
                     /locus_tag="PLK68_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM90075.1"
                     /translation="MNFFWKLYFSIMAITLTCFSVGGYMLIQTSFDNSFEREVEAVYQ
                     ENDILVNSLTLELLPHLEELTIKGGTFQERLDLLKNLVSNMTVETFNGNVSFCIRAEN
                     GDVVYQNDTFNDDHKLFDKISQNERGYIVQKDKEHYKLQSLRIFSLNGTKLYFENARD
                     ISELFLNRENQFGTLFYYTIILLLASGVVIFAVTRWLVSPIKKLSKATKGVADGHLIV
                     PVVVSSEDEIGQLTKDFNTMTERLSTTMKELHEAVERQEVFVGNFAHELKTPLTSIIG
                     YGDMLRSKRLNEEEVIGYSNLIVEEGRRLEAMSMKLLELIVLKKQDFKMYWVTAETFF
                     QGIVDTVDLLMKEQQIEFIIEIEPGKLYIEPDLMKTVCLNLLDNARKAVGQNGKVYLR
                     GKVLATGYQFVVKDNGCGIAATELSKIKEAFYMVDKSRSRSAGGAGLGLAICEQIIEL
                     HHASINFESVLGQGTTVTVVLEGVKVDEV"
     gene            complement(3323..3979)
                     /locus_tag="PLK68_06400"
     CDS             complement(3323..3979)
                     /locus_tag="PLK68_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM90076.1"
                     /translation="MNKILIVEDEQAICRLIKINLSDAGYSCKCAYDGKGAIELIEYN
                     QFDLILLDIMLPEINGYELMEYIRPLEIPVIFLTAKGDVKDRVKGLKLGAEDYIVKPF
                     EIIELVARVETVLRRYHKTSTVLSVYDISVDTLSRVVKKNDQVINLTVKEYDLLLLFI
                     QNKNIALFRDRIYEAVWGDYYMGDSRTVDLHVQRMRKKLGLEDKIVPVYKVGYRLEGD
                     "
BASE COUNT         1335 a          877 c          473 g         1448 t
ORIGIN      
        1 gcaggaaagc tttttaacga tttgatacca attgcaaatt atcaatctgc ttttgttgat
       61 ctatgtaata ttcataagca aaataaccgc caacacatgc tccggtaata ataagcaaga
      121 tcaataatga taaaataaaa gttgaaaact tagaacgtcc ttgtttttcc tttttcactt
      181 tagttttagt ctttgttttt cgctttgttt ttgttacttt ctttttctta gtttttggac
      241 gacgttgctt tttatttccc gtctcctcca ccgcttcatc ttgttctggc tcatctatat
      301 ctaactcctc taaatcatca tcaaactcaa atctttttgt cacttatatc acctcacgta
      361 taaatattat acactgatgt gaatttaata taaactttta tcattttcct ttattttaca
      421 tattcttcta aacacacacc cagctcattt atttttttag cgtgttcttt cccaacataa
      481 cgataatgcc atggttcata ccctacccct gtaatactat ttttattagt cggatagcgc
      541 aaaacaaacc catacttcca actattttgg attagccatt gttgttcaac tgtgttttcc
      601 tggctacgat caaggcgctg attttttgtt gaaacaatat ctactgctaa acctaactga
      661 tgttcactag ttcccggtct tgccacccaa aaagctgctt tatcgctagc ttccaccttt
      721 gaatatccct gacttaaata ttcacttact ttattttgat aaagttgttc ttgcttctca
      781 tttgtccgat aggaagaaca aatcaatgga tttaatccct cacttttagc cgcttgtaac
      841 attgctatta aatcatcgta aattcgccga tcgactgatt gcccattatt caattgcact
      901 aaatcaactt tccaatcttt aggtatcgta ttttcaaaat taaccaatgt taataattca
      961 tctttaacca cactattagc cgatttattt tggttagttt taaccttatt agcagctatc
     1021 agctgattat tactttgatt aacttgatcg ttaactaaaa taaataaact cataaccaac
     1081 ccaatcatca acaaagtaac tactaacact ttcattttat taatctgttt cacttcacct
     1141 tacccctttc aataaaaaaa catctctttt acactttcaa gtgtataaaa gagatgcata
     1201 gaaacatcac caaagaagca tcaaagttgt atcaataaat atcattataa ctagaaacac
     1261 ttactgaatc ggtgcgatta tcaattattc cgaataaata cttataagtt tcttgttcag
     1321 tcaaaccaag atatgctgtt ccaaaagtat aaatcacttc ataatttctt gctatatatt
     1381 ttttattata ataattatat tgatatatta agtgcgtatc aacatctata gtggcattaa
     1441 agattccttc ttcattcgta aaattcacct gataaacact gacactttta ctagaatcaa
     1501 tcacattact atagcgtctt aatgtataat tataacacac ataatttccg tcaaaattaa
     1561 aatcagttaa aataccaaga ttcttcaata taactaattg ctcattaatg atcgttcgga
     1621 ttctcgtaat attttcatca ctcatatctt gaacttgtgt cgtagtaata atattttttt
     1681 ccttattttc atagtcacca atcaactcta atttttcagc gaccgtcaac ttacttgttt
     1741 gaatttcatt attatcactt tttatttttt cgacctgaag atgctcaatt aatttatcat
     1801 cttgtatttt agaaactgtt aacggaacta acggaatgat gatcaacaaa attattgcag
     1861 ctgcaaattt aataatattt ttactatacc tcatcaactt tcactccttc taaaacaacg
     1921 gttacagtag ttccctgtcc caaaacactt tcaaaattaa ttgatgcatg atgcagttca
     1981 ataatctgtt cacatatcgc taaacctaac ccagccccgc cagcacttcg tgaacgtgat
     2041 ttatcaacca tgtaaaatgc ttctttaatt ttactcaatt ccgttgctgc aataccacaa
     2101 ccattatctt tgacaacaaa ctgataccct gttgccaata cctttcctct taaatatact
     2161 ttaccgtttt gtccaactgc tttacgggca ttatcaagta aatttaaaca cactgtcttc
     2221 attaaatctg gttcaatata caattttcca ggctcaattt caataataaa ctcaatttgc
     2281 tgctctttca tcaataaatc tacagtatct acaattcctt gaaaaaatgt ctcagctgta
     2341 acccaataca ttttaaaatc ctgttttttc aaaacaatca attcaagcag tttcattgac
     2401 attgcctcaa gacgtcttcc ttcttcaaca atcaaattag aatagccaat aacttcttct
     2461 tcattcaaac gtttcgaacg taacatatca ccataaccaa tgatcgacgt cagaggtgtt
     2521 tttaattcat gggcaaaatt tccgacaaat acttcttgac gttcaaccgc ttcatgcagt
     2581 tctttcatcg ttgtactcaa acgctcagtc atagtattaa aatcttttgt taactgaccg
     2641 atctcatcct cactgctaac tactacagga acaatcaaat gtccatcagc caccccttta
     2701 gtagctttag acagcttttt gattgggcta accaaccatc tagttacagc aaaaataact
     2761 actccactag ctaataataa tataatcgta taataaaaca gtgttccaaa ttgattttca
     2821 cgatttaaaa aaagttcaga aatatctcgc gcattttcaa aatacaactt agtcccattc
     2881 aaagaaaaga ttcgaagaga ttgtaactta taatgctcct tatctttttg aacaatatac
     2941 ccccgctcat tttgagatat tttatcaaac aatttatggt catcattaaa agtatcattt
     3001 tgatatacta catctccatt ttccgctcga atacaaaacg aaacattacc attaaatgtc
     3061 tcaactgtca tattagaaac taagttttta agcaaatcaa gccgttcttg aaaagttccg
     3121 ccttttatcg taagttcctc tagatgtggg agcaattcta aagttaacga attaactaaa
     3181 atatcatttt cttgataaac agcctccact tcgcgttcga aggaattatc gaagctcgtt
     3241 tgaatcagca tatagcctcc cacactaaaa caggtgagcg ttattgccat gatactaaaa
     3301 tacagcttcc aaaaaaaatt catcaatctc cctccaaacg ataaccaact ttgtaaactg
     3361 gtacgatttt atcttccaat ccaagctttt ttcgcattcg ctgtacatga agatcaacag
     3421 ttcggctatc tcccatataa taatcacccc ataccgcttc ataaattcga tcacggaaaa
     3481 gagcaatatt tttattttga ataaacaaca gtagcaaatc atattctttt actgttaggt
     3541 tgattacttg atcatttttc ttcactactc gtgaaagtgt atctacacta atatcataaa
     3601 cacttaaaac tgttgatgtt ttatgataac ggcgcagtac cgtttctacc cgagcaacta
     3661 attcaataat ctcaaaaggc ttaacaatat aatcctcagc tcccagtttc aatcccttaa
     3721 cccgatcttt tacatcacct ttagccgtta aaaaaatcac tggaatttct aaaggacgaa
     3781 tatattccat taattcatag ccattaattt ctggtaacat aatatctagt aaaattaaat
     3841 caaattgatt atactcaatt aattcaattg ctcctttccc atcataagca catttgcagc
     3901 tatatccagc atctgataag ttaatcttaa ttagtctaca aattgcctgt tcgtcttcaa
     3961 cgatcaaaat tttattcata gtatcacctc tattactttc atcgtacatc aaaattgcat
     4021 cttattagca tcatttcaca aaaaaacaca cttacttagt aagtgtgtta actccattat
     4081 ggagcgggtg atgggaatcg aacccacgta gtcagcttgg aaggctgaag ttc
//
LOCUS       DAOMTA010000190         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_675_length_4129_cov_2.513255, whole genome shotgun sequence.
ACCESSION   DAOMTA010000190 DAOMTA010000000
VERSION     DAOMTA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_675_length_4129_cov_2.513255"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLK68_06405"
     CDS             complement(<1..1172)
                     /locus_tag="PLK68_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM90077.1"
                     /translation="MEEKEKSVICWPLIGLLLLLCFISCLGIKSATPLITKGNPSGYW
                     IKQLMFYGISFILMFIVYKVSNDRIYSSMWIIYGILMVLLVGLAIDHFAFTRFGIHIV
                     PLAKWAGGATSWYNLKVFDLQPSEFMKIIMVVVMADTVDKHNNRYLVHNIHNDCLLIG
                     KLLAISLPPCILVYLQNDAGVTMIMLASVVFVIFMSGIQAGWFIIGGIVVAIILGIGV
                     YLFLYQHDIFASLMGGDHKLNRFYGWVDPEGTYNDQGFQLFNAMLSYGTAGLWGHGMG
                     TAIINLPEAQTDFIFAVIALGFGFVGGGFTIAVVCVLDALLIRIGFKSKNNRDKYLTA
                     GIFGLLIFQQVWNIGMVLGLFPITGITLPFLSYGGSSLLSYMIAMGIFLDMERQTR"
     assembly_gap    1313..1322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1323..1583
                     /locus_tag="PLK68_06410"
     CDS             <1323..1583
                     /locus_tag="PLK68_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HRM90078.1"
                     /translation="KQPKQVSAADIPVDVNSIIEAVGGKTNIKETTATSSKVTFFVND
                     DSLVDLDKLKALGASGIVQTSNKVSAILGKYSKEISHMINGQ"
     gene            1629..>1996
                     /locus_tag="PLK68_06415"
     CDS             1629..>1996
                     /locus_tag="PLK68_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HRM90079.1"
                     /translation="MDIKDRVKSYEPLFNQWLFEELVEIHDQESLIKIKHQAIGVEKT
                     ALLKVITLYDQDNNIEFLEKRVEEIKNRLGLQVINESEYLDCEQYLIEDNQGKIIGID
                     LCLLMVDEVDRLQNINNVSG"
     assembly_gap    1997..2006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2007..3885
                     /locus_tag="PLK68_06420"
     CDS             <2007..3885
                     /locus_tag="PLK68_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM90080.1"
                     /translation="SYFAGKLLYELGLKLFDKHEYLEALKYFKKGAEVDNSDCVCIIG
                     YLYERGLGVEQDHYQAFRYYQSATSLGNVVASCNLAYFYELGIGVKQDYQKAFELYEF
                     GARAGFARAICNLGYCYEYGHGTAVDLPRAVNYYYQAAKLGYSEALYSLGTCLEFGEG
                     IEQDIERAFKCYEEAANQGHERSQHRLGYCYENGLGTIQDFVKAFYWYYQASQVNYAP
                     ALIALASCYELGQGTDIDLKAARQNYIKAARQGYSRAQFWLGYFYENHPEIKNAPYRC
                     SYWYRQASKQNDVQAIVALGYCYESGFGVKQNLIKAIELYNKAANQGYAPAQCNLAYC
                     YEMGIGIDVDLNEAVRYYRLAGDAGYPRALCNLGYLYDHGEGVDVDHHQAFKLYQKAA
                     EANYAPGLYHLALAYEEGNGVDVDIDKAIEYYELASHQDYGIALYNLGLIYEHNEQYH
                     DDLKAIKYYEAAIDQNDVRAMYRMALYLDEGKVIAKNLDKAFTYLQIAANQGYGPAMN
                     MYGIYLENGIGGYKNLDEAFKYYLASTSDEYVPGIYNLARCYFYGIGTTVDKASAFKL
                     FLKASERGYYDASFMIGYMYSYGDGINQDKQKAKEYFKQAANKGMKEAIEELNKLDKE
                     V"
     gene            3934..>4129
                     /locus_tag="PLK68_06425"
     CDS             3934..>4129
                     /locus_tag="PLK68_06425"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM90081.1"
                     /translation="MSFKEKLSKYAQLIVKAGLNVQPAQIVVIDAPVESVELVRLVTK
                     EAYAAGAREVVVKYNDEVVSR"
BASE COUNT         1428 a          573 c          824 g         1284 t
ORIGIN      
        1 attcgcgttt gtcgttccat atctaagaaa attcccattg caatcatata tgaaagaagt
       61 gatgaaccac cgtaggatag aaatggtaag gtgattccag taattggaaa tagtcctaaa
      121 accattccga tattccaaac ttgctggaag ataagtagac caaagattcc ggctgtgaga
      181 tatttatccc gattattttt agatttaaaa ccaattctaa ttaataaggc atcaagtaca
      241 cagacaactg caatagtgaa accgccaccg acaaatccaa atcctaaggc aataacagcg
      301 aaaataaagt ctgtttgtgc ctcaggaaga ttaatgatag ctgttcccat accatgtccc
      361 cataggccgg cagttccata tgataacatt gcattaaaca gttggaaacc ttgatcatta
      421 taggtaccct cgggatcgac ccaaccatag aaacgattta atttatggtc tcctcccatt
      481 aaactagcaa aaatatcatg ttggtataag aatagataaa ctccaatccc aaggatgatt
      541 gcaactacaa tcccccctat gataaaccag ccggcttgaa taccggacat aaaaataacg
      601 aatactactg atgcaagcat gatcattgtt actccggcat cattttgtaa ataaacgagg
      661 atacaaggtg gtaaggaaat agctaagagc ttaccaatca ataaacaatc attatgaata
      721 ttatgtacta aatagcgatt attatgttta tcgactgtat ccgccatgac aaccaccatg
      781 atgattttca taaactctga tggctggaga tcaaatactt tgagattgta ccaggatgtg
      841 gcaccacccg cccatttagc taaaggaaca atatgtattc caaatcgcgt aaatgcaaag
      901 tgatcaatag caagaccaac tagtaaaacc attaaaattc cataaatgat ccacatcgat
      961 gaatagattc gatcgttgga gactttataa acgataaaca tcaagataaa tgatattcca
     1021 taaaacatta gctgtttaat ccaatagcca cttggatttc ctttagtaat caaaggagtg
     1081 gcgcttttaa ttcccagaca gctaatgaaa caaagcaata gtaatagacc gattaatggc
     1141 caacagataa cggatttttc tttttcttcc acaaaatccc tccttataaa aatgatagtt
     1201 ttttctatca tggtatagta tactataatt aaatggtaat taataggggg tatattttaa
     1261 tgaatgaata tttattatat ggaataatcg cactgattgt aatcgtaata ttnnnnnnnn
     1321 nnaagcaacc aaagcaagta tcagctgctg atattccagt tgatgtaaat agtattatcg
     1381 aagctgtcgg cggtaagacg aatattaaag aaacaacagc tacttcttca aaagtcactt
     1441 tctttgtaaa tgatgatagt ttggttgatt tagataagtt aaaagcactt ggtgcttctg
     1501 gaatcgttca aacaagtaac aaggttagtg caattttagg aaagtattca aaagagatat
     1561 ctcatatgat taatggacag taagcagata acaatatctg cttatttctc tatcgaaagg
     1621 aggtaagtat ggatataaaa gatcgtgtta aaagttatga gccgttattt aatcaatggt
     1681 tgtttgaaga actggtagaa attcatgatc aagaatcatt gataaaaatt aaacatcaag
     1741 cgattggtgt tgagaaaacg gctcttttaa aagtaataac attatatgat caagataata
     1801 atattgaatt tttagaaaaa cgtgtagaag agattaaaaa tagattagga ttgcaagtaa
     1861 tcaatgaaag tgaatattta gattgtgaac aatatcttat tgaggataat caaggaaaga
     1921 taattggtat tgacctatgt cttttgatgg ttgatgaagt tgatcgttta caaaatataa
     1981 ataatgtcag tggtaannnn nnnnnngtct tattttgctg gtaaattatt gtatgaatta
     2041 ggtttgaaat tatttgataa acatgaatat cttgaagctt taaagtattt taaaaaggga
     2101 gcagaggtgg ataatagtga ttgtgtttgc attattggtt atttatatga acgtggacta
     2161 ggtgtcgaac aagatcatta tcaggctttt cgttattatc aatcagcaac aagtttagga
     2221 aatgttgtcg caagctgcaa tttagcatat ttctatgagt taggaatcgg tgtgaaacaa
     2281 gattatcaga aagcttttga gttatatgaa tttggggctc gagctggatt tgctcgtgct
     2341 atttgtaatt taggctattg ttacgaatac ggtcatggaa ctgctgttga tcttccacgt
     2401 gctgttaatt attattatca ggcggctaaa ctagggtata gtgaggcttt gtatagctta
     2461 ggaacttgtt tagaatttgg tgaaggaatt gaacaagata ttgaacgtgc tttcaaatgt
     2521 tacgaagaag ctgcgaacca agggcacgaa cgttctcaac accgtctggg ttattgttat
     2581 gaaaatggat taggaacaat tcaagatttt gttaaagcat tttattggta ttatcaagct
     2641 agtcaagtta attacgctcc tgctttaatt gcgttagcgt cgtgctacga attaggtcaa
     2701 ggaactgata ttgatttaaa agctgcacgt caaaattata taaaagctgc acgtcaagga
     2761 tattcgcgag cacagttttg gttgggttat ttttatgaga atcatcctga aattaaaaat
     2821 gcaccatatc gctgttcata ttggtatcgt caggcaagta aacaaaatga tgttcaggca
     2881 atcgtagcac taggttactg ttatgaaagt ggttttggag ttaaacaaaa tttaatcaaa
     2941 gcaattgagc tatataataa agcagctaat caagggtatg ctccagccca atgtaattta
     3001 gcatattgtt atgaaatggg aattggtata gatgtcgact taaatgaagc tgtccgctat
     3061 tatcgcttgg ctggagatgc aggttatccg cgggctttat gtaatttagg ttacttatac
     3121 gatcatggag aaggagtgga tgttgatcat catcaagctt ttaagttata tcaaaaagct
     3181 gcagaagcta attatgctcc gggtctttat catctagctt tggcatatga agaaggtaat
     3241 ggagtggatg ttgatattga taaagcaatt gagtattatg aattagcaag tcatcaagat
     3301 tatggaattg ccctttataa tttaggtttg atctatgagc ataatgaaca gtatcatgat
     3361 gatttaaagg caatcaaata ttatgaagct gcaattgatc aaaatgatgt ccgagctatg
     3421 tatcggatgg ctttatattt agatgaaggt aaagtaattg ctaaaaatct agataaagct
     3481 tttacttatt tgcagattgc tgctaatcaa ggctatggac cagctatgaa tatgtatgga
     3541 atatatcttg aaaatggaat tggtggatat aaaaatttag atgaagcttt caaatactat
     3601 cttgcttcaa caagtgatga atatgtaccg ggaatatata atttagcacg ttgttatttt
     3661 tatggtattg gaacaacagt tgataaagct tcagctttta aattgttttt aaaggctagt
     3721 gaacgtgggt attatgatgc tagttttatg attggctata tgtatagtta tggagatgga
     3781 attaatcaag ataaacaaaa agccaaagag tattttaagc aggctgccaa taaaggaatg
     3841 aaagaggcga ttgaagagtt gaataagctc gataaagagg tataaagaag tttagattga
     3901 ttataataaa ttgatggaaa gaggtagata aagatgtcgt ttaaagaaaa attatcaaaa
     3961 tatgcacaat tgatcgttaa agctggttta aatgttcagc cagctcaaat tgttgtaatc
     4021 gatgcacctg ttgagagtgt tgaattagta cgtttagtaa ctaaggaggc atatgcggct
     4081 ggagctaggg aagtggttgt aaaatataat gatgaagttg tttcacgat
//
LOCUS       DAOMTA010000191         4123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_677_length_4123_cov_2.468289, whole genome shotgun sequence.
ACCESSION   DAOMTA010000191 DAOMTA010000000
VERSION     DAOMTA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_4123_cov_2.468289"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLK68_06430"
     CDS             complement(<1..442)
                     /locus_tag="PLK68_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM90082.1"
                     /translation="MKKSSYELLGQDPKWVKKLLFAGVFIQENKLHAIFDRYNEMTSK
                     QWLLMATMTSFTDAPDLSMLAKVMGCSRQNVKKLALSLEKQGYVILEKSPKDARSLCV
                     VMSKQGLRFRKDMEELTDEVHQALFSEFSNEEITQYYQLSIKLMH"
     gene            complement(586..1146)
                     /locus_tag="PLK68_06435"
     CDS             complement(586..1146)
                     /locus_tag="PLK68_06435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM90083.1"
                     /translation="MTEREKMELGMWYDANNDEELLKQRVLAKDLCFDLDHIKPSSYE
                     KRLEVITSLLGYQPINIELISPFMCDYGNKIKIGKNAFINSNCYFMDGGGIELGDNVY
                     IGPSCGLYTAIHPTEYKIRNTGLEQALPIKIGNNVWLGGNVVILPGVTIGDGCVIGAG
                     SVVTKDIAPNSVACGNPCKVIRSIEN"
     gene            complement(1149..1619)
                     /locus_tag="PLK68_06440"
     CDS             complement(1149..1619)
                     /locus_tag="PLK68_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HRM90084.1"
                     /translation="MKPKKRILHERLAAVVASLRHGEMLFIADAGSGTSSKALYPLDS
                     SVEYIDVEVVTGSPSFEDIVTTLVACGDFEGAIVTEDMIEVNQKDYQLLVDLLGKKNI
                     HSMHYIPEYYEMRDRCKAVVQTGDYGVHAQAILIAGYPSAEISMKWLKEGIKGK"
     gene            complement(1850..2032)
                     /locus_tag="PLK68_06445"
     CDS             complement(1850..2032)
                     /locus_tag="PLK68_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90085.1"
                     /translation="MLRGQKYAKAYKLEIVKEHLNNHKSFLELKEKYGFICLLDEQYY
                     HQIYQFDVQYQKIKRL"
     gene            complement(2104..3093)
                     /locus_tag="PLK68_06450"
     CDS             complement(2104..3093)
                     /locus_tag="PLK68_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM90086.1"
                     /translation="MNRIGTGKLRDLNDQIILKLLLRHQEVTKKELANYSRLTVATVG
                     TILNDFLANGTVVEKEIIYLKKGRPTKKYGLNPEYFHSLCLFVQRKRGRDYLCWQIID
                     ALASVLKQGKELVNDLKLEDILKFIKYLLSQDSRIQIIGLGVPAIISKEIVIESDIPN
                     LKNVNLKAEIEKATGLKTVVKNDMNYTAYGCYLHNNKKDLCYVTFPLNSGPGCGSVIN
                     GKLIEGENSIAGEILYLPFFKYLKQEQLCFDYSPEDVALSLCCVASIINPSIVILTGE
                     AIEADDILKIKKICLEYLPSEFMPELTYCANYENDYLLGIQEVIRESFIKQDK"
     gene            3341..>4123
                     /locus_tag="PLK68_06455"
     CDS             3341..>4123
                     /locus_tag="PLK68_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRM90087.1"
                     /translation="MKTWLITGCSSGIGRGIAKAVLKKGDNAVVTARDTSKIADLVDQ
                     YPNTALGVALDITKKESISAAVKQAQERFGTIDVLINNAGYGYRSSVEEGDIDDVNLL
                     FNTNFFGPIELIKEVLPQMRANKNGAIVNVSSIAAVRSGVGSGYYAASKAALELMTDG
                     LAKELKPLGIKVMIAQPGSFRTNFYDTSLKGTKNKIDDYNETAGKTRKENVVNHKNQP
                     GDPDKGGQVIVDTIEKDNYPFRLLLGSDATKIVASALEERIQE"
BASE COUNT         1383 a          756 c          520 g         1464 t
ORIGIN      
        1 catgcattaa tttaattgat aactgataat actgagtgat ttcttcatta ctaaattcac
       61 taaataaagc ttgatgtacc tcatctgtta attcttccat atcttttcta aagcgcaatc
      121 cctgtttact cattaccaca cataacgaac gtgcatcttt gggtgacttc tctaaaatca
      181 catatccctg cttttccaaa ctcagtgcta atttttttac attttgtcgt gaacacccca
      241 tcacttttgc taacatgctt aaatcaggag catcagtaaa tgaagtcatc gtagccatta
      301 ataaccattg tttactcgtc atttcattgt aacgatcaaa tatcgcatgt agcttattct
      361 cctgaataaa cacacccgca aataacagtt ttttgaccca tttaggatcc tgtcctaata
      421 attcataact tgattttttc atcattctct ccatatccgt aactagttac ttatttttat
      481 tatagtaact agttaccaat ctgtcaataa acaaatactt tcatcagatt atttcataat
      541 aaaaagttat aatgacttct cactataacc tctaaaacag atattttaat tttcgattga
      601 tcttattact ttacagggat ttccacaagc aacactatta ggagcaatat ctttagtcac
      661 aacactgcct gctccaatta cacagccatc gccaatcgtt acccctggta aaataactac
      721 attaccacct aaccacacat tattaccaat tttaattggt agcgcttgtt ctaaaccagt
      781 atttctaatt ttatattcgg taggatgaat agcagtatat agaccacatg aaggtcctat
      841 ataaacatta tctcctaatt caattccacc accatccata aaataacaat tactattaat
      901 aaaagcattt ttaccaattt taatcttatt tccataatca cacataaagg gacttataag
      961 ctctatgttt ataggttgat aacctaacaa acttgtaatc acttctaatc ttttttcata
     1021 actgctaggt tttatgtgat ctagatcaaa acacaaatct ttagctaata ctctttgttt
     1081 taataattct tcatcattat ttgcatcata ccacattcct aattccattt tttcacgttc
     1141 tgtcattttt atttcccctt tattccttct tttaaccatt tcatactaat ctctgcacta
     1201 ggataccctg caattaaaat tgcctgtgca tgtacaccat aatcacctgt ttgtaccact
     1261 gctttacaac gatcacgcat ttcataatat tcaggaatgt aatgcatcga atgaatattt
     1321 ttctttccca acaaatcaac cagtaactga taatcttttt gatttacttc aatcatatct
     1381 tctgtgacga ttgctccttc aaaatcacca caagcaacta gtgtagttac tatatcctca
     1441 aaacttggtg aaccggtcac tacttctaca tctatatatt caacacttga atccagtggg
     1501 tatagggctt tactagatgt tccactgcca gcatcagcaa taaataacat ctcaccatga
     1561 cgcagactag caacaactgc tgccaaacgt tcatgaagaa ttctcttttt tggtttcaca
     1621 cttatctcct tatttattaa aagtctttta ttaattaata attataagtt atctaataaa
     1681 aaagtcaaca aataaaacaa ctgtccttga accgtcctaa tcaaataaat agatgaaaca
     1741 atcaaattat tagatttgaa gttatcaaaa ataataactt catttattat ttagaaataa
     1801 acaggttaag ctatgcaaat attattacta tagtgttcag tctcatattt cataatcttt
     1861 ttattttctg gtattgaaca tcaaactgat aaatctgatg ataatactgt tcgtccaaca
     1921 aacatataaa tccatatttc tctttcaatt caaggaaaga tttatgatta tttagatgtt
     1981 ctttgacaat ttctaattta tacgctttag catacttttg tcccctcaac aatataaaag
     2041 cttcctattc aatgtttaat tatttacact atctaggagg tttttatatc aataacagtt
     2101 gttttatttg tcttgtttaa tgaaactttc tctaataact tcctgtattc ccaataaata
     2161 atcattttca taattagcac aatacgttaa ttcaggcata aattcacttg gtaaatactc
     2221 taaacaaatc ttcttgattt ttaatatatc atcagcctca atagcttcac ctgttaaaat
     2281 cacgattgaa ggatttatta tcgatgctac acaacataat gataaagcca catcttctgg
     2341 tgaataatca aagcataact gttcttgttt taggtattta aaaaatggta aatataagat
     2401 ttcaccagca atactatttt ctccttctat taatttacca ttaataacac tcccacagcc
     2461 aggtccagaa tttaatggaa acgtcacata acaaagatcc tttttattat tatgtaaata
     2521 gcagccataa gcagtataat tcatatcatt tttaacaact gtctttaatc ctgtagcctt
     2581 ctcgatttca gctttaagat ttacattttt taaattaggg atatctgatt caattactat
     2641 ctctttagaa attattgccg gtacccctaa accaattatt tggattctcg aatcttgtga
     2701 taaaagatat ttaatgaatt ttaaaatatc ctctaatttt aaatcattta ctaattcttt
     2761 accctgtttt agtacacttg caagggcatc aatgatttgc cagcaaagat aatctctgcc
     2821 tctttttctt tgtacaaata aacacaatga atgaaaatat tctggattca atccatattt
     2881 tttagtagga cgaccttttt ttaaataaat aatttctttt tcaacaacag ttccattagc
     2941 caaaaaatca ttaagaatcg ttcctactgt agctactgtc aatcttgaat aattagctag
     3001 ttcttttttt gtcacttctt gatgtcttaa taacaatttc aaaatgattt gatcatttaa
     3061 atctcgtagt tttccagttc caattctatt cacatttatc agcccttttc aattattttt
     3121 gattatatca gtatttttaa aagaaaaata ccctttgtct ccaaagggct tttaattcaa
     3181 gtacgtcatt aacacattca ttattatatg ataaaaatgt cttttgggca actgacaaaa
     3241 agagaatatt taattttttt atttttatat tgacttatag ttaactctaa gttctatact
     3301 ataaatgtag aaaagataaa tacatgaagg aggacttaaa atgaaaacat ggttaattac
     3361 aggatgctct agcggaattg gacgcggcat tgctaaagcg gtattaaaga aaggggataa
     3421 tgctgttgtt actgctcgtg atacaagtaa aattgccgat ctagttgatc aatatccaaa
     3481 tacagcttta ggagtggctc tagatattac caaaaaagag agtattagtg ctgctgtaaa
     3541 acaagcacaa gaaagatttg gaacaattga tgtattgatc aataatgctg gatatggata
     3601 tcgttcttca gttgaagaag gtgacataga tgatgttaat cttttattta atactaactt
     3661 ctttgggcca atcgaactaa ttaaagaagt attaccacaa atgcgagcta acaaaaacgg
     3721 tgctattgtc aacgtatcat caattgcagc ggttagatcg ggtgtaggtt ctggttatta
     3781 tgctgctagt aaggctgctt tagaattaat gactgatggt ctagctaaag aattaaaacc
     3841 gctaggaatc aaggtcatga tagcacaacc aggttctttc cgaactaatt tttatgatac
     3901 ttcactaaaa gggactaaaa ataagatcga tgattataac gaaacagctg ggaaaactcg
     3961 taaagagaat gtcgttaatc ataaaaatca acctggtgat cctgataaag gtggtcaagt
     4021 tattgttgat acaattgaaa aagacaacta tcctttccgt cttctcttag gtagtgatgc
     4081 taccaagatc gtagctagtg ctttagaaga aagaattcaa gaa
//
LOCUS       DAOMTA010000192         4113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_679_length_4113_cov_2.609414, whole genome shotgun sequence.
ACCESSION   DAOMTA010000192 DAOMTA010000000
VERSION     DAOMTA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_4113_cov_2.609414"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1825
                     /locus_tag="PLK68_06460"
     CDS             320..1825
                     /locus_tag="PLK68_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90088.1"
                     /translation="MDILMKYAKKKTVFILSGIVTLLYLISKFGPTTSSNVNINDINE
                     LAGTIGSMVTIIQIIFYLFIILAIVTAILSGVYFFKKNKQEYVMLGEFIVSCINSILL
                     LLSMSGINAICKILRVAISGDYSSLMTMNSARLLSSVTSAASNLKYFMYLSIFVFIIN
                     IVLLLIVKKIINIDGFHFNFDEPVVTAVPAQEPVHEDSTTVEDDSTIADNELTQENGE
                     TETNTVEPNASETKPATINTQKIKDFFKTKNGKITIGVAAALIVCFGGYKIYDTFFNY
                     TSIDLGKNITVEFTGKDGSGYIKDVESNIDYDKNNSDLSNFVSSTYTDYDFSGDLSNG
                     DKITVTIKYSEELAKANKIKVTNDSKTFTVKGLIEKFKDSSKIPEKVITQLKKEADKN
                     IKNRYKDGYSFTYNHEFNSLWFAKGEDDDNDSVIAIYKIDETYTSSFSGQQDVETYYV
                     DDVDSAYLDEKSHYWYGASLYGSDGKELSDLNALETALKEKFDDQTLELIK"
     gene            complement(1917..2735)
                     /locus_tag="PLK68_06465"
     CDS             complement(1917..2735)
                     /locus_tag="PLK68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM90089.1"
                     /translation="MLVIVIILIILLLIATCYIFNLKKSIRKAANDINKIRGLDTNAL
                     ITTTNGNLELRELLQEVNEMMLTFRKLEIDIEKKNNSLQKTIVNISHDLKTPLTSALG
                     YVELLQKYELSGEEQHKYESIIIDKLKRLSQLIDDFFTFTKIISNEEKFELKLLDINR
                     IFEEELVCYYQDFNSQGRMIYIEGNKKIEMLSNERILRRIFDNLIINALKHSRGDIYI
                     SINTGAEICFQFKNRLDEPIIVEQIFDEFYTSDISRTKQNTGLGLAIVKEFTEM"
     gene            complement(2751..3563)
                     /locus_tag="PLK68_06470"
     CDS             complement(2751..3563)
                     /locus_tag="PLK68_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90090.1"
                     /translation="MLNIIKADLFRIFKGKGIYVCLLAIIALCSVSIYLRSPGHIGIS
                     GGLTSNSEVTKEDDLATPEDVRKVAGESNELLDEGMMKVNGNLYYVLIFVVFAVLCVD
                     LANHTAKNVISTDVSRTTYYFAKLLLTWGLGIAIIAISTYLGYFGNIIFNAPSHYSSF
                     LDITIIMLRQLPIFCGIMSVLVMIAAITQKTSRYNAIAIVLVMVSQMLLMTIITVFNI
                     DGSIIMQFEFETILRDMAVIGQIEIKTLLTGIGLIIASSMIGVTYFKRCNIK"
     gene            complement(3565..>4113)
                     /locus_tag="PLK68_06475"
     CDS             complement(3565..>4113)
                     /locus_tag="PLK68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter"
                     /protein_id="HRM90091.1"
                     /translation="NCKKITDFSMVGSCFNLKKIDLRGCFLNNLDFIILDEPINGLDP
                     LGISEIRETLLQLQQENITILISSHILSELYLVANKFGILEGGKIVKELSKEQLDNEC
                     SHCLLLKVNDIKKASVLLEEELKTANYKVLNDQEIRLYDYLDAQHIVSKAIVEKGIEL
                     YAISEIGVSLEEYFKNIIRDGE"
BASE COUNT         1464 a          668 c          509 g         1472 t
ORIGIN      
        1 aatggaatcg ctgaaaaaaa ggcaacgtac ggtggaaaac tagtcgtttt atgatgttca
       61 atcaaacaat attgatatgc cacaacaata cataataaat gtgataaaaa taaagtcaac
      121 cccaaaaaac tataatacat atactgataa aatttcattt catccacctc catatgactt
      181 tgttatgatt catttattcc ttcttttatc attataacat agtatttttt atcaattccc
      241 atgattccct atttatttac aaaatcttta gtaatatgtt atcttttttg ataatatatt
      301 agtataggag ggaattatta tggatatttt aatgaaatat gctaaaaaga aaactgtttt
      361 tattttgtca gggatagtta cattattgta tttaatctca aaattcggtc caacaacatc
      421 atctaatgtt aatatcaatg atattaatga attagctgga acgattggat caatggttac
      481 aattattcaa attatatttt atctatttat tatattggcc attgtaaccg ccattcttag
      541 tggagtttat tttttcaaaa aaaataagca agaatacgtg atgctagggg aattcatcgt
      601 ttcatgtatc aactcaatat tattactatt atcaatgtcc ggaatcaatg ctatttgtaa
      661 gattcttaga gttgcaatta gtggtgatta ttcatcactt atgactatga attcagcacg
      721 attacttagt tccgtaacta gcgctgcaag caaccttaaa tattttatgt atttatcaat
      781 ttttgtattt attatcaata ttgttttatt attaattgtt aaaaaaatta ttaatattga
      841 tgggtttcat tttaattttg atgaaccagt tgtaactgct gttccagctc aagaacctgt
      901 tcatgaagat tctacaactg tcgaagacga ttctacgata gctgataatg agttaacaca
      961 agaaaacgga gaaactgaaa ctaacacggt tgaacctaat gcttctgaaa ctaaaccggc
     1021 aactatcaat acacaaaaaa tcaaagactt ctttaaaaca aaaaacggaa aaataacgat
     1081 tggtgttgct gctgctctta ttgtttgctt tggtggttat aaaatatatg atacattctt
     1141 taattatact agtattgatc taggtaaaaa tattactgtt gaatttaccg ggaaagacgg
     1201 aagtggttat ataaaagatg ttgaaagcaa tatcgattat gacaaaaata attctgattt
     1261 atctaacttt gttagcagca cttatactga ctatgatttt tctggagatc tatctaatgg
     1321 tgataaaata actgtaacga ttaaatactc agaagaattg gctaaagcaa ataaaatcaa
     1381 agtaactaat gattctaaaa cttttactgt taaaggtcta attgaaaaat ttaaagatag
     1441 cagtaaaata ccagaaaaag ttattactca attaaaaaaa gaagctgaca aaaacataaa
     1501 gaatcgctat aaagatggtt attcttttac ttataatcat gaatttaatt ctctttggtt
     1561 tgccaaaggg gaagatgatg ataacgacag tgttattgct atatataaaa ttgacgaaac
     1621 atatacctca tcattcagtg gccaacaaga tgttgaaact tactatgtcg atgatgttga
     1681 ttctgcctat ttagatgaaa agtcacatta ttggtatggg gcttctttat atggcagtga
     1741 tggtaaagaa ttatcagatc taaatgcatt ggaaactgct ttaaaagaaa aatttgatga
     1801 tcaaacttta gaattaatca aataaaaaac taaaaccact ttttaaaata tttaagaagt
     1861 ggttttttta ttccaaatca aactgattat taaattattt tcacttatct tagcagttac
     1921 atctcagtaa attctttaac aatagctaat cctaagccag tattttgttt tgtacgagaa
     1981 atatcagaag tataaaactc atcgaatatt tgttcaacta tgattggctc atctaatcga
     2041 tttttgaatt gaaaacaaat ttctgctccc gtattaatag aaatataaat atctccacgg
     2101 ctatgcttca aagcattaat aattaaatta tcaaaaattc tccgtaagat ccgttcatta
     2161 ctaagcatct ctattttttt attaccttca atatagatca tccgcccttg actattaaaa
     2221 tcttgataat aacaaactaa ctcttcttca aaaatacgat taatatctaa taactttaat
     2281 tcaaattttt cttcattgga aatgatttta gtaaaagtaa aaaaatcatc aatcaattgc
     2341 gatagccgtt ttaacttatc aataataatt gattcatatt tatgctgttc ttctccactt
     2401 agctcatatt tttgcaacaa ttcgacatag cccaaggccg aagttaatgg cgtttttaaa
     2461 tcatgagaaa tatttacaat tgttttttgc aaactattat ttttcttttc aatatcaatc
     2521 tctaattttc taaacgttag catcatttca tttacttctt gtaataactc tctaagttca
     2581 agattcccgt tagttgttgt aatcaaagca ttagtatcaa gccctcttat tttattaata
     2641 tcattagccg cttttctaat actttttttt aaattaaaaa tataacaggt agcaataagc
     2701 agtaaaatga tcaaaattat cactattact aacattcagc tacctcctga ttatttaata
     2761 ttgcatcgtt taaaatacgt tactcctatc atacttgaag cgatgatcaa tccaattcct
     2821 gttaacaatg tctttatttc tatctgtcca ataactgcca tgtcccgaag aattgtttca
     2881 aactcaaatt gcatgattat actaccatca atattaaaaa cagtaataat tgtcattaat
     2941 agcatctgtg ataccataac taacacgatt gcgattgcat tatatcgtga tgttttttgt
     3001 gttattgcgg caatcattac tagtacgctc attataccac aaaagatcgg caactgtcgc
     3061 agcataatta ttgttatatc taaaaagcta gaataatgac tcggtgcatt aaagataata
     3121 ttaccaaaat aacctaaata agtactgatt gctattattg ctatccctaa tccccatgtc
     3181 aaaaggagct tagcgaaata gtatgtcgtc cgtgaaacat ctgtagagat aacattttta
     3241 gcagtatgat tagctaaatc aacacaaaga actgcaaaga ctacaaaaat cagtacatag
     3301 tacagattcc cattaacttt catcatccct tcatctaata gctcattact ctcaccagct
     3361 acctttctta catcttctgg agttgctaaa tcatcttctt tagttacttc tgaattactc
     3421 gttaacccgc cacttattcc aatatgacct ggcgatctta aataaattga aacactgcac
     3481 agtgcgatga ttgctagaag acaaacataa attccttttc ctttaaaaat cctaaataaa
     3541 tcagctttaa tgatattcaa cattctattc accatcccta ataatatttt taaaatactc
     3601 ttctaacgat actccaattt cactaattgc ataaagttca attccctttt caactattgc
     3661 cttactaaca atgtgttgtg catctaaata atcatataga cgaatttctt gatcatttaa
     3721 gactttgtaa ttagctgtct ttagttcttc ttctaataag acactagctt tttttatatc
     3781 atttacctta agtaataaac aatgactaca ctcattatct aactgttctt ttgataattc
     3841 tttaacgatc ttaccacctt caagaattcc aaacttattt gctactaaat acagttctga
     3901 taaaatatga ctagaaatca aaatcgttat attttcttgt tgtaattgca ataacgtttc
     3961 tctaatctca ctaattccta atggatctaa accattgatt ggttcatcta aaattataaa
     4021 atcaagatta tttaaaaaac atcctcttaa atcaattttt tttaaattaa aacaagaacc
     4081 taccatcgaa aaatctgtaa tttttttaca gtt
//
LOCUS       DAOMTA010000193         4110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_681_length_4110_cov_2.348952, whole genome shotgun sequence.
ACCESSION   DAOMTA010000193 DAOMTA010000000
VERSION     DAOMTA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_681_length_4110_cov_2.348952"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PLK68_06480"
     CDS             <1..1169
                     /locus_tag="PLK68_06480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM90092.1"
                     /translation="TCGHILVANSKAIELANITSEAVEGGYFDLDAGLFQENALYLIY
                     DTIPQPTIEEIKDNILIAQKELHSYGITSVQSDDLLSATSDYHDALQAFEQLRAENKL
                     TIRVYEQAQLPTLKALKEFINLGYCTGSGDDFFKIGPLKMLGDGSLGARTAFLSKPYY
                     DAPKTRGIPVFSREEIKMMFDYANRHEMQIAIHAIGDGILDWIFEGYENALKNYSRED
                     PRHGIVHCQITREDQLLKYQQLHLHAYIQSVFLDYDNHIINQRVSPQLAQTSYNFKTL
                     RNIATISNGSDCPVEAPDVLKGIQLAVTRTSIDGTGPYLKEQALTREEAIESFTIGGA
                     YASFEEEVKGTLEVGKYCDFVVLSDNILDVDVQHIKDIKVLATYVGGQLVYGGQ"
     gene            1170..1295
                     /locus_tag="PLK68_06485"
     CDS             1170..1295
                     /locus_tag="PLK68_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90093.1"
                     /translation="MRVNEQAVKNILKSVGNAKLVAATKYVGIDDSACNQYSQPD"
     gene            1258..1839
                     /locus_tag="PLK68_06490"
     CDS             1258..1839
                     /locus_tag="PLK68_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRM90094.1"
                     /translation="MIQLVTNTANQINELEKLGVKYFGENRVQAFLEKYEKYHGAQEF
                     HMIGTLQPNKVKYIIDKVSLIHSIDSYSLIKEVEKQAKKHDLKMPILIQVNVAKEESK
                     HGFAVEEIEDVFDYLKECPHLIVKGLMMMAPHIDSNDTAVYFKQTKELFDQLNCKYSE
                     FEMTELSMGMSNDYHEALKYGATMIRVGSALFE"
     gene            2048..2797
                     /locus_tag="PLK68_06495"
     CDS             2048..2797
                     /locus_tag="PLK68_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HRM90095.1"
                     /translation="MEAWENYFEVMEKVVAQVKNTQKDNIKKAAKILADTTEKGGLIY
                     GFGTGHSHLVVDDAFWRAATPANYCALLEQSATGSFEITKSYYIENMYEIGKMIVDYH
                     RITPNDCMIIISNSGNNIAPVDAAIRAKEKGIPIIAITAVEYSQFLKTKHKAGVKLKD
                     VADVVLDNCSLIGDAAIEIENFPMKVGATSTIPNVFLQNAILCEMVDILVKKGIHPDV
                     YYNGHMAFMNEDCADHNDKLVDKYFYRIRNL"
     gene            2794..3540
                     /locus_tag="PLK68_06500"
     CDS             2794..3540
                     /locus_tag="PLK68_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM90096.1"
                     /translation="MMKARSRYYQHIHDILNNIVDSQEAAIQKAALEMTQCIEKGHTI
                     YAFGASHAGILTQELFYRAGGLALINPIMAKEVQLDVRPITLTSQMERLPGYGTLILE
                     HTPIQKEDVLIIHSVSGRNTIAIDMALRAKAMGVIVIAVTNIEYSQAVVSRHETGKKL
                     LDIADIVIDNCGDFEDSSITIDGLDQKVAPTSTIAGAFILNSVIIQVVENLVNDGFEP
                     PIFHSANIDGGDEYNQNILNQYKNRIHYMK"
     gene            3590..>4110
                     /locus_tag="PLK68_06505"
     CDS             3590..>4110
                     /locus_tag="PLK68_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM90097.1"
                     /translation="MNTRSVEILKKLCGGKNYQVEDLAHDFEISIRMIRYVIDDINDF
                     LETINIKKNIMIRNGKIQFNITHQEKEILMKEISNLDNYVYAISPFERKCYILVSMWC
                     CSEPLTSQYFADEMNVSKSSIDKDILSIRQEYEGYDFSINVKTGKGSYIEGDERDIRS
                     CFFRVIEKSSVNG"
BASE COUNT         1477 a          513 c          828 g         1292 t
ORIGIN      
        1 ggacttgtgg acatatctta gttgctaata gcaaagcaat tgaattagca aatattacca
       61 gtgaagcagt cgagggcggc tattttgatt tagatgcagg attatttcaa gaaaatgcac
      121 tttatttgat ctatgataca atccctcagc caactattga agaaataaaa gataatattt
      181 taatcgctca aaaagagtta catagttatg gaataacatc tgttcaaagt gacgatctct
      241 tgagtgccac tagtgattat catgatgctc tacaggcttt tgaacaatta agagcagaaa
      301 ataaactaac gataagagtt tacgaacaag cacagttgcc gacgttaaaa gccttaaaag
      361 agtttattaa tttaggttat tgtaccggta gtggtgatga tttttttaaa ataggcccat
      421 taaaaatgct tggggatgga tcattaggag cacgaactgc ttttttaagt aagccttatt
      481 atgatgcacc taagacacga ggaattcctg tttttagtcg tgaagaaatc aaaatgatgt
      541 ttgactacgc taatcgtcat gagatgcaga ttgctattca cgcaattggt gatggaatct
      601 tagattggat ttttgaagga tacgaaaatg cgttaaaaaa ctattctcgt gaagatccac
      661 gacatggtat tgtgcattgt cagatcactc gagaagatca gttgcttaaa tatcagcagt
      721 tacatttaca tgcttatatt caaagtgtct ttttagatta tgataatcat attattaatc
      781 aaagggtttc tccccaacta gcacaaacat catataattt taaaacactt cgtaatatag
      841 cgactatttc taatgggtca gactgtccag ttgaagcccc agatgtttta aaagggattc
      901 aattagcggt tacaagaaca tctatcgatg ggactggacc atatctaaaa gagcaggcat
      961 taactagaga agaagcgatc gagtcgttta cgatcggtgg tgcatatgct tcatttgaag
     1021 aagaggtcaa aggaactttg gaggtaggaa agtattgcga ttttgttgtg cttagtgata
     1081 atatattaga cgtagatgtt cagcatatta aagacattaa agtattggct acttatgtag
     1141 gtgggcaatt agtctatgga ggtcagtaaa tgagagtaaa tgaacaggca gtaaaaaata
     1201 ttttaaaatc ggttggtaat gctaaattgg tcgcagcaac aaaatatgtt ggaattgatg
     1261 attcagcttg taaccaatac agccaaccag attaatgaat tagaaaaact aggagtaaag
     1321 tattttggcg aaaatcgagt ccaagctttt ttagaaaaat atgaaaagta tcatggggca
     1381 caagaatttc atatgatagg aacgctacaa ccaaataaag ttaaatatat aattgataaa
     1441 gttagcttga ttcattcaat tgatagttat tcattaatta aagaagtaga aaaacaagct
     1501 aagaagcatg atttaaaaat gccaatatta atccaagtca atgtagcaaa agaggaaagt
     1561 aagcatggtt ttgcagtaga agaaattgaa gatgtatttg attatttaaa agaatgtccc
     1621 catttaattg taaaagggct aatgatgatg gcaccacata ttgattctaa tgatacagca
     1681 gtatatttta aacaaacaaa ggagttattt gatcaattaa attgtaaata ttcagagttt
     1741 gaaatgacag aattatcaat ggggatgtct aatgattatc acgaagcgtt aaagtatgga
     1801 gcaacgatga taagggtagg aagtgccttg tttgaataaa gtaaagttta tagagtcgcg
     1861 gagtttatta atttaaaact ccttttttca ttataaaata taatgtattt taagagattt
     1921 ggatattatt tcaaatttct ttttttattg aacttgtaaa atgcttctca agaaattgaa
     1981 agaatttgca aatatttgct cttttgaatt taggtataat gaaattgtaa aggaaggagg
     2041 cttttaaatg gaagcttggg aaaattattt tgaagttatg gaaaaagttg ttgcacaagt
     2101 aaaaaatacg caaaaagata atatcaaaaa agctgcaaaa atacttgctg atacaactga
     2161 aaaaggaggt cttatttatg ggtttgggac gggtcattct catttagtag tcgatgatgc
     2221 attttggcga gctgctaccc cggctaatta ttgtgctttg ctggaacaaa gtgcaacagg
     2281 cagttttgaa attacaaaaa gttattacat tgaaaatatg tatgaaattg gaaaaatgat
     2341 agttgattat catcgaatca cccctaatga ctgcatgatt attatttcta attctggcaa
     2401 taatattgca ccagtagatg ctgctataag ggcaaaagaa aagggaattc ctattattgc
     2461 gattactgca gtggaatata gccaattctt aaagacaaaa cataaggcag gagtaaagct
     2521 taaagatgtt gctgatgtag tgttagataa ttgttcatta ataggtgatg cggcaattga
     2581 gatagaaaat tttcctatga aagtgggggc aactagtaca ataccaaatg tctttttaca
     2641 aaatgcaata ttatgtgaaa tggttgatat tttagttaaa aagggaattc acccggatgt
     2701 ttattacaat ggtcatatgg cttttatgaa tgaagattgt gctgatcata atgataaatt
     2761 agtggataaa tatttttatc ggataaggaa tttatgatga aagcaagatc aagatactat
     2821 cagcatattc atgatatttt aaataatatt gtagattcac aagaagcagc tattcaaaaa
     2881 gctgccttag aaatgaccca atgtattgaa aagggacata cgatttatgc ttttggagct
     2941 tctcatgcgg gaattcttac ccaagaattg ttttatcggg ctggtggatt ggctttaatt
     3001 aatccaatta tggcaaagga agttcaatta gatgtaagac caataactct aacctcgcaa
     3061 atggagagac tacctggtta tgggacattg attttagagc atacgccaat acaaaaagaa
     3121 gatgttctaa ttattcattc agtttctggt cgaaatacaa ttgcaattga tatggcatta
     3181 cgggctaagg caatgggtgt gatcgtgatt gctgttacaa atatagaata tagtcaagct
     3241 gtagtttcgc gacatgaaac aggaaagaaa ttgttagata ttgcagatat tgttattgat
     3301 aattgtggag attttgagga ttccagtatt actattgatg gtctagacca aaaagttgcc
     3361 ccaacctcta caatagccgg agcttttatt ttaaatagtg tgattattca agtagtagaa
     3421 aatctagtta atgacggctt cgaaccaccg atttttcata gtgccaatat tgatggaggt
     3481 gacgaatata atcaaaatat cttaaaccaa tataaaaatc gcattcatta tatgaagtga
     3541 gtagagagga ggcgtatgat tctcttattg aatcatatga ggatatctta tgaatactag
     3601 aagtgtcgaa atattgaaga agctgtgcgg cggaaaaaac tatcaagtag aagatttagc
     3661 acatgatttt gagatctcta ttcgaatgat tagatatgtt attgatgata ttaatgattt
     3721 tttagaaaca ataaatatta aaaaaaatat aatgataaga aatggaaaga ttcagtttaa
     3781 tattactcat caagaaaaag agatactgat gaaagaaatt tcgaatttgg ataattatgt
     3841 atatgcgata agtccgtttg aaagaaagtg ctatatttta gtttcaatgt ggtgttgcag
     3901 tgaaccactc acaagtcagt attttgctga tgaaatgaat gttagtaaaa gtagtattga
     3961 taaagatatt ctttcgataa gacaagaata tgagggatat gatttttcta ttaatgtaaa
     4021 aacaggaaaa ggcagttata tagagggtga cgaaagagac atacgctcat gtttctttag
     4081 agttattgaa aagtcttctg ttaatggctg
//
LOCUS       DAOMTA010000194         4075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_695_length_4075_cov_2.318408, whole genome shotgun sequence.
ACCESSION   DAOMTA010000194 DAOMTA010000000
VERSION     DAOMTA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_695_length_4075_cov_2.318408"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLK68_06510"
     CDS             complement(<1..644)
                     /locus_tag="PLK68_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90098.1"
                     /translation="MGSILIKLHQLINYNQNENIHYSLAKYLLANCDTINRLSISKVV
                     EDTLISKTSVLKFCRYLGYDSWKVFCSDFQEECLDEKIRLDRLKMNANLMFSKDNLNE
                     YVRLNDEYFSEVQKIVTFQKIKIFAKQINEANNIFVFGEPRELPLFYDFQELLGLYHK
                     ELIFPKSLNKKDFEEQLALIDQESLIIITNGVHSFDGFIEKETIEPVYGLERIM"
     gene            complement(647..1429)
                     /locus_tag="PLK68_06515"
     CDS             complement(647..1429)
                     /locus_tag="PLK68_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90099.1"
                     /translation="MNTLLSRLIVYINSCVKKDVIYEIARYIIKNYSYCQNITLKDIM
                     DECYVSRASVTRFCEYFGFHSWNNFQSFLVKTKKVKEQQIESRFSNIDIESIYDHLCY
                     IAKNDSLEFKNNLKKEINALVEIINQSSRIYLFGAVYPLSLAVDFQINMISIGKTVYS
                     DFQSESDYLEPMNEDDLAIILTASGRYVGECKSKFNLICNNAGKKAVISCSNRYSSLQ
                     YINHYLYIPTTNKNSFVDFDYYTILILDLIYIEYNLKYCRGK"
     gene            complement(1671..3011)
                     /gene="murD"
                     /locus_tag="PLK68_06520"
     CDS             complement(1671..3011)
                     /gene="murD"
                     /locus_tag="PLK68_06520"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRM90100.1"
                     /translation="MFAGKKVLVIGLAKSGKAAIRLLHKLNATITVNEAKEIKDIPEY
                     QQYLDMGIEMVTGSHPTELFERDFDFVIKNPGINYHKPFILRLKERKIPVYTEIELAF
                     QVAKKQHYIGVTGTNGKTTTVTLIDKILKGQYQHVHLAGNVGTPLCDVVLDNDLLNEE
                     NHYVVIEMSNFQLLDIERFKPYVSTIINLTPDHLDYMASLDEYYASKTNIYKNTDLDD
                     YFVVNLDDKTVMEYLEKYPVPCNQVTMSLIHEADCMIKEQAIYYRDELIINLADIKVV
                     GRHNIQNIMTAICIAKKCDVPVTVINREIASFIGVEHRIEFVREINGVKIYNDSKATN
                     TDATIIALKAFEQPVILLMGGFDKGLDLTEMATYGNKISHLVTFGAAGERFKNDMHVA
                     NSSSVENLEAATLLAIEKAKAGDIILLSPSTSSFDEFSGYEERGRVFKNIVNKL"
     gene            complement(3015..3992)
                     /gene="mraY"
                     /locus_tag="PLK68_06525"
     CDS             complement(3015..3992)
                     /gene="mraY"
                     /locus_tag="PLK68_06525"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRM90101.1"
                     /translation="MENFLKLIIAFGIGFILVVLAMPKVIPFLHKMKFGQVEREEGLE
                     SHKSKNGTPTMGGIVFVIAAILGAFIVNFNNLLDPELILATIVLVGYSAIGFVDDALI
                     IVKHSNKGLPPLAKLLAQIALAIICYFFAMNFIPDFTSVITIPLLDINIDMGYLYPAL
                     ILVMFAGESNGVNLSDGLDGLATGLSMVAIAPFIIFSIMTKDYTLASYATAMVGALLG
                     FMMFNYHPAKIFMGDVGSLGLGGFLAILAILTKQELLLILVGGVFLMETLSVIIQVVS
                     FKTRGKRVFKMAPIHHHFEMLGWSEQQVTISFWFIGFICGILSIVIGVL"
BASE COUNT         1445 a          734 c          484 g         1412 t
ORIGIN      
        1 tgcataattc tttctaatcc atataccggt tcaatagttt ccttttcaat aaacccatca
       61 aatgaatgaa ctccgttagt aataattatt aaactttctt gatcaatcaa agctaactgt
      121 tcctcaaaat cctttttatt taaagattta ggaaaaatca attccttatg atataacccc
      181 aataattctt gaaaatcata aaacagcggt aattctcgag gttctccaaa gacaaagata
      241 ttatttgctt cattaatttg tttagcgaat atttttattt tttgaaatgt aacaattttt
      301 tgtacttcac taaaatattc atcgtttagt cgaacatact catttaaatt atcttttgaa
      361 aacattaagt ttgcattcat ctttaagcga tccaagcgaa tcttttcatc gagacattct
      421 tcttgaaaat cactgcaaaa gactttccaa gaatcatatc ctaaataacg gcaaaatttt
      481 aaaacagatg tttttgaaat caaggtatct tcaacaactt tgctaattga taatcgattg
      541 atcgtatcac aattagctaa taaatatttt gctaacgaat aatgaatatt ttcattttga
      601 ttgtaattga ttaattgatg taatttaatt aagattgatc ccactattat ttccctcgac
      661 aatactttaa attatactca atataaatta aatctaagat caatatcgta taataatcaa
      721 aatcaacaaa actattttta ttagtcgttg gaatgtagag ataatgattg atgtattgaa
      781 gtgagctata ccgattactg cagctaataa ctgctttctt gccagcatta ttacaaatta
      841 aattaaactt ggacttacat tcaccaacat atcttccaga agcagttaaa ataatcgcta
      901 gatcgtcctc attcattggc tctaaataat cactttcact ttgaaaatca ctataaactg
      961 ttttaccaat actgatcata tttatttgga aatcaactgc caatgataat ggatatactg
     1021 ccccaaataa ataaattcta ctactttgat taattatttc aactagtgca ttgatctctt
     1081 tttttaaatt atttttaaat tctaaactat catttttagc aatataacat aaatgatcat
     1141 agatactttc tatatcaata ttactaaaac gactttcaat ttgctgttct ttaacttttt
     1201 ttgttttaac taaaaagctt tgaaaattat tccaagaatg aaaaccaaaa tattcacaaa
     1261 atctcgttac actagctctc gaaacataac attcatccat aatatcttta agcgtaatat
     1321 tttggcaata actataattt ttaataatat accgggctat ttcgtaaatc acatcttttt
     1381 ttacacatga attgatatag actatcaatc tgctgagtaa tgtattcatg ccttcctcct
     1441 ttataaaata atatttatct ataaacaggt agcactgaaa cgacattaag ataacttacg
     1501 attacccata agcttgcaat ttttaatgtt accatgcata tacgtataat tttattatat
     1561 aatgggaaat tttcaaatag aaagtttttc ataaattaaa aaagacactt tcgacaataa
     1621 aataaacctt taagctatat tccttaaagg ttcactttac aaagcatctt ctataattta
     1681 ttaacaatat ttttaaatac tcgaccacgt tcttcatatc cagaaaattc atcaaaactg
     1741 ctggttgatg gcgaaagtaa aataatatcg ccagcttttg ctttctcgat tgccagcaaa
     1801 gtcgctgctt ctaaattctc aacagaacta ctgtttgcaa catgcatatc atttttaaat
     1861 cgctctccag cagcaccaaa tgtcaccaga tgactgattt tattaccata agtagccatt
     1921 tcggttaaat ctaacccttt atcaaaacca cccatcaata aaattactgg ttgttcaaaa
     1981 gcttttaaag caatgatcgt agcgtcagta ttagttgctt ttgaatcatt atagatttta
     2041 actccattaa tttctctaac aaactcaatt cgatgttcaa caccaataaa tgaggcaatt
     2101 tctcgattga tgactgttac tggtacatca cattttttag caatgcagat agctgtcata
     2161 atattttgaa tgttatggcg tcctacaact ttaatatccg ctaagttaat aattaattca
     2221 tctcgataat agattgcctg ttctttgatc atacagtcag cttcatgtat caatgacatt
     2281 gtgacttgat tacatggaac tggatatttt tccaaatact ccattactgt tttatcatca
     2341 agattaacca caaaataatc atccagatca gtattcttat aaatatttgt tttagaagca
     2401 taatactcat ccaatgaggc catataatct aaatgatcgg gtgttaaatt gatgatcgtt
     2461 gagacataag gcttaaatcg ttcaatatct aataactgaa agttactcat ttcaattact
     2521 acataatgat tttcttcatt aagaagatca ttatctaaaa caacatcaca tagtggtgtt
     2581 cccacattac ctgctaaatg aacatgttga tattgtccct ttaatatttt atcaattaaa
     2641 gtaactgttg ttgttttacc attagtacca gttactccaa tataatgttg ttttttagca
     2701 acttgaaaag ctaattcaat ttccgtatat acaggaatct ttctttcctt aagtcttaaa
     2761 ataaatggct tatgataatt aattccaggg ttcttgatta caaagtcaaa atcacgttca
     2821 aataattcag ttgggtgtga ccctgttacc atttcaattc ccatatcaag atattgttga
     2881 tattctggaa tatccttaat ttctttagct tcattaaccg taatcgttgc atttaattta
     2941 tgaagcaatc taatcgcagc cttaccactt ttagcaagtc caatcactaa tacctttttt
     3001 ccagcaaaca tattctatag caccccaatt acaattgata agattccaca gatgaaacca
     3061 atgaaccaga atgaaatagt tacttgttgt tcactccagc ccaacatttc aaagtgatga
     3121 tggatcggtg ccatcttaaa tactcgtttt ccccgagttt taaatgagac tacttgaata
     3181 ataaccgata aagtttccat taaaaagact ccaccaacta aaataagtaa taactcttgt
     3241 tttgtaagta tcgctaaaat agccaagaaa ccacctagac caagcgaacc tacatcaccc
     3301 ataaatatct tagcaggatg ataattaaac atcatgaaac caagtaaagc ccctaccatt
     3361 gcagttgcat aacttgcaag agtataatct tttgtcatga tagaaaagat tataaatggt
     3421 gcaatcgcaa ccattgaaag accagttgct aatccatcta aaccatcaga taaatttaca
     3481 ccgttacttt cccctgcaaa cattactaag attaatgctg gatataaata acccatatca
     3541 atattaatat ctaataatgg aattgtaatt actgatgtaa aatcaggaat aaagttcatt
     3601 gcaaagaaat aacaaataat tgctaacgcg atctgagcta ataatttagc tagtggcggt
     3661 aatcccttat tactatgttt aacaattatc aaagcatcat caacaaatcc aattgcacta
     3721 tatccaacta aaacaattgt tgctaaaatc agctccggat caagtaaatt attaaaatta
     3781 acaataaaag caccaagaat tgcggcaatc acaaaaacga ttccacccat tgttggtgta
     3841 ccattttttg acttatgtga ttcaagtcct tcttcacgtt caacttgacc aaatttcatc
     3901 ttatgtagga atggaattac tttaggcatt gctagaacaa caagaataaa gccaattcca
     3961 aaagcaatta ttagttttaa gaagttttcc atctatatgt cccctttttt ataaaattcg
     4021 tctttactat atcatattat cgtttaatag gcaaaataaa agggacaaga gtccc
//
LOCUS       DAOMTA010000195         4051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_704_length_4051_cov_2.407157, whole genome shotgun sequence.
ACCESSION   DAOMTA010000195 DAOMTA010000000
VERSION     DAOMTA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_704_length_4051_cov_2.407157"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /gene="argS"
                     /locus_tag="PLK68_06530"
     CDS             <1..1483
                     /gene="argS"
                     /locus_tag="PLK68_06530"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRM90102.1"
                     /translation="PRQIAEAIIEALDKENANIEKVEIAGPGFINLFLAKDAMTSIIK
                     EVLEEKEAYGTTTYGQGTKYNVEFVSANPTGDLHLGHAKGAAVGDSICRIMSAAGYDV
                     TREYYINDAGNQIHNLALSLYARYKQEFGQDVTMPEDGYHGKDIIDIATKIKEIDGDK
                     YLEMDEGKAIAFFRNKGTEYELQKIKDILNEFRVSFDVWFSETSLYENDRVVPTIEKL
                     KAAGYTYEEEGALWFKSTEFGDDKDRVLIKSDGSYTYLTPDIAYHLNKLDRGYEYLVD
                     LLGADHHGYINRMKAAIQALGYNADQLNIDIIQMVRMMNNGEPVKMSKRTGNAVTIKD
                     LIEEIGVDATRYFFVSKAANTPFDFDIGLAKSKSNENPVYYAQYAHARMCSIKAQAAK
                     ANIDYSDKYDLLVNPKEIELVKHINEFRNVIIDSAINRTPHKITNYVQRLAQLFHSFY
                     NECYVIDEDNLELSGQRLALVEATRITMANALNLIGVSAPEKM"
     gene            1498..1839
                     /gene="rpoE"
                     /locus_tag="PLK68_06535"
     CDS             1498..1839
                     /gene="rpoE"
                     /locus_tag="PLK68_06535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HRM90103.1"
                     /translation="MDSMDKYYNMSMVDVAYELMSKKKSAVNFFKLWEEVCQMKKFDD
                     EQKEDKESLFYTNITLDGRFITVGENAWDLRSRHKFSDVHIDMNDIYADEEETEELEE
                     DVDSTIEDDYN"
     gene            1945..2601
                     /locus_tag="PLK68_06540"
     CDS             1945..2601
                     /locus_tag="PLK68_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRM90104.1"
                     /translation="MLKSIKKTNLLMMSSIILFMAFITIYLFRNCDFNDVIYEIETLP
                     YFTKVSAMIALIALQIFLAFLPGEPLELASGYIFGSFQGTIVCLIGSMIGTIIVYYLA
                     RIFQHSIIDKMFDQSKVAEVKKLFSSKKSKFWLFIIFLIPGSPKDIMTYLVSLTDIDI
                     KQWLLLTTIGRIPSIVTSTYLTGALRDGNIVLAVSIFAVTVILVITGAVYYKKIVNSN
                     "
     gene            2618..3321
                     /locus_tag="PLK68_06545"
                     /pseudo
     CDS             2618..3321
                     /locus_tag="PLK68_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            3325..>4051
                     /locus_tag="PLK68_06550"
     CDS             3325..>4051
                     /locus_tag="PLK68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM90105.1"
                     /translation="MTKAKRIFGVIFASILLLMSVVVISLYDNVRENTPRDDNLTGIL
                     EDDLQLFTIAMAKTLDSNYEAVSFDDSVDEQTRQIFLDVFDDYLKGITSLFNNDGDFI
                     YKAVNTNTNQMISQHSEKITTNDDQSKYSFYTEASYDQNGLVSCNGDLSSDKLSYFNI
                     ANLLSSFAHYDGENTIYIDNWMFDTNAIKINVPKNLQITYIIPETPASHGYASQYVNS
                     WENYNYFSAIALLSCSAILVLFIL"
BASE COUNT         1452 a          465 c          769 g         1365 t
ORIGIN      
        1 tcctcgccaa attgctgagg caattattga ggctttagat aaagaaaatg ccaatattga
       61 aaaagtagag attgcgggac cgggatttat taacctattc ttggcaaaag atgcaatgac
      121 atcaatcatt aaagaagttt tagaagaaaa agaagcatat ggtaccacaa catatggtca
      181 aggaactaaa tataatgttg aatttgtttc agctaatcca actggcgatt tacatctagg
      241 acatgctaaa ggggcagcag tgggtgatag tatctgccga attatgagtg ctgctggcta
      301 tgatgtgact cgtgaatatt atattaatga tgcagggaat caaattcata atttagcgtt
      361 atctctatac gctcgttata aacaagaatt tggacaagat gttacaatgc ctgaagatgg
      421 ttatcatggt aaagatatta ttgatattgc aacaaagatc aaagagatcg atggtgataa
      481 atatcttgaa atggatgaag gtaaagcaat tgctttcttt agaaataaag gaacagaata
      541 tgaattacaa aagattaaag atattttaaa tgaatttaga gtgtcgtttg atgtttggtt
      601 ttctgaaaca tcattatatg aaaatgatcg ggtcgtacca acaattgaaa aattaaaagc
      661 tgctggttat acatatgaag aagaaggggc tttatggttc aaatcaactg aatttggtga
      721 tgataaagac cgggtcttaa ttaaaagtga tggatcgtat acatatttga cgcctgatat
      781 tgcttatcat ctaaataaat tagatcgtgg atacgaatat ttagttgatt tattaggggc
      841 tgatcatcat ggttatatta atcgaatgaa agcagctatt caagctttag gttataatgc
      901 tgaccagtta aatattgata ttattcaaat ggttagaatg atgaataatg gtgaaccagt
      961 aaaaatgtca aaacgaactg gaaatgctgt tactattaaa gacttaatcg aagaaattgg
     1021 tgttgatgct acacgttatt tctttgtttc taaagctgct aatacaccat ttgattttga
     1081 tattggactt gctaaatcaa agtctaatga aaatccagtt tattatgctc aatacgctca
     1141 tgctagaatg tgttcgatta aagcacaggc tgctaaagca aacattgatt atagtgataa
     1201 gtatgattta ttagttaatc ctaaagaaat tgaattagta aagcatatta atgagtttag
     1261 aaacgtaatt attgatagtg cgatcaatcg tacgcctcac aaaattacaa attatgttca
     1321 aagacttgca caattattcc atagttttta taatgaatgt tatgtaatcg atgaagataa
     1381 tcttgaatta agtggacaaa gattagcttt agttgaagct acaagaatta caatggctaa
     1441 tgctttaaat ttaatcggtg ttagtgcccc agaaaagatg taggaggata atcaagaatg
     1501 gatagtatgg ataaatatta taatatgtct atggttgatg tagcatatga attaatgtct
     1561 aaaaagaaat cagcagttaa ttttttcaaa ttatgggaag aagtatgtca aatgaagaaa
     1621 tttgatgacg aacaaaaaga agataaagaa tctttgtttt atactaatat taccttagac
     1681 ggacgtttta tcacggttgg tgaaaatgcc tgggatttaa gaagtcgtca taaatttagt
     1741 gatgttcata ttgatatgaa tgatatttat gctgatgaag aagaaacaga agaattagaa
     1801 gaggatgtcg attctacgat tgaagatgat tataattaag caaaacgaaa gttttgcttt
     1861 ttgtttagaa ttttttaaga attattgtta gtttagtttt aggatagtct tttattattt
     1921 aagtatagaa aggcggaacg acttatgctt aaatcaataa aaaagacaaa tttgctcatg
     1981 atgtcatcaa ttattttgtt catggcattt attacaatat atttatttag aaactgcgat
     2041 tttaatgatg ttatttatga gattgaaaca ctgccatatt ttactaaagt ttcagcaatg
     2101 attgctttaa ttgctctgca gatcttttta gcatttttac ctggggaacc tttggagctg
     2161 gctagtggat atatttttgg ttcatttcaa ggaacaatcg tttgtttgat cgggtcaatg
     2221 attggaacga ttattgttta ctatttagca cgaatctttc aacactcaat cattgataaa
     2281 atgtttgatc aaagtaaagt tgcagaagtg aaaaaattat tttcttctaa aaaaagtaag
     2341 ttttggctat ttattatttt tttgattcct ggaagtccta aagatattat gacttattta
     2401 gttagcttaa cagatattga tataaaacaa tggctattgc ttacaacgat tggaagaatt
     2461 ccaagtattg tgacatctac atatttaacg ggagcattaa gagacggcaa tattgtattg
     2521 gctgtttcaa tctttgcggt aactgttata ctagtaatta ctggtgccgt ttattataaa
     2581 aaaatagtta attctaatta gaaaaaggga gaaaattatg gaacatacaa ttttaattgt
     2641 tgaagatgaa aaaggaatca gagaaacagt aacagttttt ttgaagaatc aaggatataa
     2701 tgttttacaa gctagtaatg gtgaagaagg gttagcatta atcgaagcaa atgaaataca
     2761 tttagcagtg gttgatatta tgatgccagt tatggatgga attacaatgg tactaaaact
     2821 acgagaaaac tatgattttc cagttatttt cttaagtgca aagtctgaag atattgataa
     2881 aataaccggg cttaatattg gtggtgatga ttatattact aagccgtttg aggcgatgga
     2941 attgatagct cgagttaatt cgaatcttcg aagatataat caaattttag ctttaaaaga
     3001 aaatcgtaat cttaataata atcaacagtc gtgggtggat tagagctgga taagtttact
     3061 aaagaagtgt ttgttaatga tcgaggtgtt cgtttaacgg ctaaagaatt tcaaatactt
     3121 gaattattaa tgtcttatcc gggaagagtc tattcagctg aggagattta tgaagctgtt
     3181 tggaaagaag aagccataaa tactgagaca attatggtgc atgtgcgaaa attgcgtgag
     3241 aaaatagaag caaatcctaa aaaaccagaa tatttaaaag tagtttgggg aataggatat
     3301 aaaattgaaa aaggagtgta aaaaatgaca aaagctaaaa gaatttttgg tgtgattttt
     3361 gcatcaatat tactattaat gtccgttgtt gttatatcct tatatgataa tgttagagaa
     3421 aatacccctc gtgatgataa tttaacggga attttagaag atgatctaca gctttttact
     3481 attgcgatgg ctaagaccct tgattctaat tatgaagcag taagctttga tgatagtgtt
     3541 gatgaacaga caagacaaat atttttagat gtttttgacg attatttaaa gggaataaca
     3601 tcattgttta ataatgatgg agattttatt tataaagctg ttaatactaa taccaatcaa
     3661 atgatttcgc aacatagtga aaaaattaca actaatgatg atcaaagtaa atatagtttc
     3721 tatactgagg cgagctatga tcaaaatggt ctagtttcat gtaatgggga tttaagtagt
     3781 gataaactat catattttaa tattgctaac cttttaagct catttgcaca ttatgatggt
     3841 gaaaatacta tatatattga caactggatg tttgatacaa atgcaatcaa gattaatgtt
     3901 cctaagaatc ttcaaattac atatattatt ccagaaacac cagccagtca tggatatgct
     3961 tcacaatatg ttaattcatg ggaaaactat aattattttt cagctattgc gttattaagt
     4021 tgttcagcta tattagtctt atttatctta t
//
LOCUS       DAOMTA010000196         4038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_710_length_4038_cov_2.463972, whole genome shotgun sequence.
ACCESSION   DAOMTA010000196 DAOMTA010000000
VERSION     DAOMTA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_4038_cov_2.463972"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLK68_06555"
     CDS             complement(<1..508)
                     /locus_tag="PLK68_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRM90106.1"
                     /translation="MNIEKWTAKMQEAIQKAITMASEMGHQVVDVEHFLLALLEDNAG
                     ILYRVLSKANVNIADLQNKLNVRLQGKPIVSNVDINSIRISYDLNQLLSLADKQMHNF
                     KDEYLSVEHLIMALFELNSSWLKEILNGYNLNKKNVKKIIDEMRGGNMVDNQNPENQY
                     EVLEKYGRD"
     gene            complement(683..1228)
                     /locus_tag="PLK68_06560"
     CDS             complement(683..1228)
                     /locus_tag="PLK68_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRM90107.1"
                     /translation="MAKFVCSVCGYVYEGDAAPEQCPVCKVGADKFVEQTGEMTWAAE
                     HVVGVAKDVDEEIKKELRANFEGECSEVGMYLAMARVAHREGYPEIGLYWEKAAYEEA
                     EHAAKFAELLGEVVTDSTKKNLEMRVEAENGATMGKTELAKKAKALNLDAIHDTVHEM
                     ARDEARHGKAFEGLLNRYFGK"
     gene            complement(1295..1846)
                     /locus_tag="PLK68_06565"
     CDS             complement(1295..1846)
                     /locus_tag="PLK68_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRM90108.1"
                     /translation="MKKVAVLFHDGFEEVEALSVVDIMRRANVECTMVGMDKLEVTSS
                     HQIKIKMDQIYDGLDNYDAVVIPGGMPGASNLRDDSRVIDLVKQFNHDGKIIGAICAG
                     PIVLQEADVIKGKTVTCYPGFEEQLIGSNYQEALVQRDENIITGKGPAAALAFGYTLL
                     EALGCDSSVIKEGMQYTYLEKNI"
     gene            2187..3374
                     /gene="coaBC"
                     /locus_tag="PLK68_06570"
     CDS             2187..3374
                     /gene="coaBC"
                     /locus_tag="PLK68_06570"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRM90109.1"
                     /translation="MKTIIVGISGSVAAYKTCDLVRALKKLNYDIEVIMTENATKFIS
                     PLTLGALINKPVLVDDFDDQGYQIKHISYAKKADCFIIVPATANIIGKIANGVCDDIV
                     TSTFLAATCPKLIAPAMNVNMYDNLATQRNIERCKTYGIKFVEPGYGLLACGDVGRGK
                     LADTDDIINMVEYCLSPKPLKHKRVLVTAGPTQEAIDPVRYITNHSSGKMGYALAKRA
                     YQLGAKVTLISGPTDLRVPYGVEVINIKSARAMFEAVKESYEKQDYIIKAAAVGDYRV
                     KEIATNKIKKHENSLILEMVKNDDILTYLGAHKTTQTICGFAMETQNLIENAKDKFKR
                     KNCDLLVANNLNENGAGFKKDTNKVTFISKNDVQSIELMSKDELSDLILKKLIKIKES
                     HSC"
     gene            3368..>3450
                     /locus_tag="PLK68_06575"
     CDS             3368..>3450
                     /locus_tag="PLK68_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HRM90110.1"
                     /translation="MLIVIDIGNTNITMGLVDKDEIIDNYRL"
     assembly_gap    3451..3465
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3466..>4038
                     /locus_tag="PLK68_06580"
     CDS             <3466..>4038
                     /locus_tag="PLK68_06580"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRM90111.1"
                     /translation="KLERTSDEYGFMLLSFLQASSILVDEIEDIIIASVVPKIMYSFT
                     NSIKKYFNKEPIIVGPGIKTGIRIKIDNPKELGADRLVDAAGAYYIHGGPCLVIDFGT
                     ATTFDVISKDGDFLGGATAPGIGISINALSSQAAKLPEIEIKKPKSVIAKNTVSSMQA
                     GIIYGYIGLTENIIREIKAEYNEKLKVISTG"
BASE COUNT         1304 a          707 c          596 g         1416 t
ORIGIN      
        1 aatcacgtcc atatttttcc aatacttcgt attggttttc aggattttga ttatcgacca
       61 tatttccacc tcgcatttca tcaataattt tctttacatt tttcttattt aaattataac
      121 catttaatat ttcttttaac cagctactat ttaattcaaa taatgccata attaaatgtt
      181 ctacacttag atattcatct ttaaaattat gcatttgctt atccgctaaa ctaagcagtt
      241 gattcaaatc atatgagatt cgaatactat ttatatccac attacttaca atcggcttac
      301 cttgcaacct tacgttcaat ttattttgta gatcggcaat attgacattt gccttactta
      361 agactcgata taatatacct gcattgtctt ccagcaaagc taatagaaaa tgctctacat
      421 caactacttg atgacccatt tcactagcca ttgttattgc tttttgaatt gcttcttgca
      481 tcttggcagt ccatttttca atattcatgt ctatcactcc tttagcactc tatatcccta
      541 agtgctaatt atattataca tctcttttta gcactgtcaa ggttaaagtg ctaaaaaatt
      601 aaaatattta tttttaaatc tacttctgtc tagatgtaaa aaactagggt ttgtcaccct
      661 agtttaatct aatatccaat tattatttac caaaatatct gtttaacaat ccttcaaacg
      721 cttttccatg acgtgcttca tcacgtgcca tttcatgcac tgtatcatga attgcatcta
      781 agtttaatgc tttagctttc ttagctaatt ctgttttacc catagttgct ccattttcag
      841 cttcaacacg catctctagg ttcttttttg ttgaatcagt tactacttct cctaataatt
      901 ctgcaaattt tgctgcatgt tcagcttctt cataagctgc tttttcccaa tataatccaa
      961 tttctggata tccttcacga tgtgcaactc ttgccattgc taaatacatt cctacttctg
     1021 agcattctcc ttcaaagttt gctcttaatt cttttttgat ctcttcatct acgtctttag
     1081 caacacctac aacgtgttct gctgcccaag tcatttcacc tgtttgttca acgaacttgt
     1141 cagccccaac tttacatact ggacattgtt ctggagctgc atctccttca taaacatatc
     1201 cacatacaga acatacaaat ttagccatat cattttcctc cttatttaac agtaatatta
     1261 taatcctatt catttaccat ttaaagtaat ttgattaaat atttttttct aaataagtat
     1321 actgcattcc ctcttttatc acactactat cacacccaag tgcttctagc aatgtgtacc
     1381 caaaagctag agctgctgct ggtccttttc cagtaataat attctcatct ctttggacaa
     1441 gtgcttcttg ataattagaa ccaattaact gttcttcaaa acctggataa caagttacgg
     1501 tttttccttt aataacatct gcttcctgta aaacaatcgg tcccgcacag attgctccaa
     1561 ttattttacc atcatgatta aattgtttaa caagatcaat aacccgtgaa tcatctctta
     1621 aattacttgc cccaggcatt cctccaggaa taacaactgc atcatagtta tctaacccat
     1681 catagatttg gtccatttta attttaattt gatgagagct agtgacttct aatttatcca
     1741 ttcctaccat tgtgcattct acatttgctc gacgcataat atctacaaca cttaaagctt
     1801 caacttcttc aaatccatca tgaaataata ctgcgacttt tttcattcaa ctcactccat
     1861 ttcttttttt attattttag caccaatcag acaaaaggta caatttaaat gattgaatta
     1921 ttattcatta agtaatataa tggctttatc atttagtgat gatcgctcat cattaatcat
     1981 ttataaatca tgattgacac cgtatctagg aagaacggta gtggaggcaa aatgaaatat
     2041 agaaaagtta attattatat attctagtat ttaatccaag ctcattccca atgaataaaa
     2101 gatggtgatg aagcatgatt gatactagaa ttgtttttaa attattaatc aaatttgaga
     2161 tagttcaaag atagaaagga agtaatatga aaacaattat tgtaggtatt agtggcagtg
     2221 ttgctgctta taagacttgt gatttagttc gagccttaaa aaaattaaat tatgatattg
     2281 aagtaattat gactgaaaat gcaacaaaat ttatcagtcc cctaactctt ggtgctttaa
     2341 tcaacaaacc tgttctggtc gatgactttg acgatcaagg ttatcaaatt aaacatatca
     2401 gctatgcaaa gaaggcagac tgttttatca ttgtccctgc aacagctaat attattggta
     2461 aaattgctaa tggtgtttgc gatgatattg ttacttcaac ctttttagct gctacttgtc
     2521 ctaagctgat tgcaccagca atgaatgtta atatgtatga caatttagca acgcagcgta
     2581 atattgaaag atgcaaaact tatggcatta aatttgttga gcctggctac gggttacttg
     2641 cttgcggaga tgttggccgt ggaaaattag ctgatactga tgatattatt aacatggttg
     2701 aatattgttt aagtcctaag ccattaaaac ataaacgtgt tttagtaact gccggtccaa
     2761 ctcaagaagc gattgatcca gttcgttaca tcactaatca ttcaagcgga aaaatgggtt
     2821 atgcattggc aaaacgcgcc tatcaattag gtgctaaagt aactctgatt agtggtccga
     2881 cagatttaag ggttccgtat ggagttgaag taatcaatat taaatctgcc cgtgctatgt
     2941 ttgaagccgt caaagaaagt tatgaaaaac aagattatat cattaaagcc gctgctgtag
     3001 gtgattatcg ggtcaaagaa attgctacta ataaaataaa aaagcatgaa aattcattaa
     3061 ttttggagat ggttaaaaat gatgatattc ttacttattt aggcgctcat aaaacaactc
     3121 aaacaatttg cggttttgca atggaaacac aaaatctaat tgaaaatgct aaggataaat
     3181 tcaaacgtaa aaattgtgat ttattagttg ctaataattt aaatgaaaat ggtgctggtt
     3241 tcaaaaaaga tactaataaa gtcactttta ttagtaaaaa tgacgtacaa tcaattgaac
     3301 ttatgtctaa agatgaatta agtgatttaa tcttaaaaaa attaataaaa atcaaggaga
     3361 gtcattcatg ttaattgtaa ttgatattgg aaatacaaat attacgatgg gattagttga
     3421 taaagatgaa attattgaca attatcgtct nnnnnnnnnn nnnnnaaatt agaacggact
     3481 tctgatgaat atggatttat gctgctaagt ttcttgcagg catctagcat attagttgat
     3541 gaaattgaag acattattat tgcctcagtt gtacctaaaa ttatgtattc atttactaat
     3601 tcgattaaaa aatattttaa taaagagccg attattgttg gtcctggcat taaaacagga
     3661 atccgaatta aaatcgataa tcctaaagaa ttaggagccg atcgcctggt tgatgctgct
     3721 ggagcatatt acattcacgg tggtccttgc cttgtaatcg attttggaac tgcgactact
     3781 tttgatgtaa taagtaaaga tggtgatttt ttaggcggtg caacagctcc cgggattggc
     3841 attagtatta atgcgctatc tagtcaggca gcaaaattac cagaaattga aatcaaaaaa
     3901 cctaaaagtg taatcgccaa aaatactgta tctagtatgc aagctggaat aatctatgga
     3961 tatattggat taactgaaaa tattattcgt gaaattaaag ccgagtataa tgagaaactt
     4021 aaagttattt ctactggt
//
LOCUS       DAOMTA010000197         4015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_717_length_4015_cov_1.937879, whole genome shotgun sequence.
ACCESSION   DAOMTA010000197 DAOMTA010000000
VERSION     DAOMTA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_717_length_4015_cov_1.937879"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..2655
                     /gene="secA"
                     /locus_tag="PLK68_06585"
                     /pseudo
     CDS             93..2655
                     /gene="secA"
                     /locus_tag="PLK68_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536295.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     assembly_gap    2656..2685
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2704..3801
                     /gene="prfB"
                     /locus_tag="PLK68_06590"
     CDS             2704..3801
                     /gene="prfB"
                     /locus_tag="PLK68_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRM90112.1"
                     /translation="MELYEIKNGLTKVHLLMTEFYQSIDIEAYRREIEGLTVITLQEG
                     FWDDANKAKVTYDKLNKMKKTTDQYDLLETTLTSLDETYELVKNTEDQEFKEILESDY
                     TVFEKELSKFETMMLLSGEHDDLNAIVEIHPGAGGTESQDWAEMLFRMYQRYAANKGW
                     KVEVLDYLDGDVAGIKSVTMLIKGDNVYGHLKAEKGVHRLVRLSPFDSAKRRHTSFAS
                     VDVMPEFNNEIEIEIQSTDLKIDTYRASGAGGQHINKTDSAVRITHLPTNIVVTCQSQ
                     RSQIQNREQAMVMLKSKLYQLMLEKQASELKELKGEQKEIAWGSQIRSYVLHPYSLVK
                     DNRSGYESNNPKAVLDGDLDGFIYAYLKSAL"
     gene            3815..>4015
                     /locus_tag="PLK68_06595"
     CDS             3815..>4015
                     /locus_tag="PLK68_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM90113.1"
                     /translation="MKKHIYETLIIIVGNFILALGICAFITPVGLITGGASGIGIAVK
                     SLTGINISYTVYAINIVMFVVGY"
BASE COUNT         1428 a          522 c          828 g         1207 t
ORIGIN      
        1 gaaacaaagt ggtgctcttt agatattcag gagcatttaa atgtcatttt cgaacaaatg
       61 gggatgaaac ctattaaaga ggagcgtatt tcatggctag tagaaaagag caaataagaa
      121 aagagttaaa aaagaaaaat gaaaaagagg gattaaatcc tgaagcagta acaaatatcg
      181 ttgatttgca agatcaaacc ggtgaagata taaaggttga agatttccaa ggaatctata
      241 atggtgaagt aattccattt aatacaacac cacattcaac aaaaaaaagc tttattggga
      301 gaattaaagg tgtttttgac gatgaacgaa aacaattaaa aaaattagat aaaatggctg
      361 acgaagtaat tgctttagaa tcaaaatatg cagcgatgag tgatgaagaa ttagcgggtc
      421 aaactagtat tttcaaagag gctctagcta atggtaaaac gcttgatgac ataattattg
      481 atgcctatgc tacagttcgt gaagctgctt atcgtcaatt aggcctaaaa gctttcaaag
      541 tacagttgat gggcggtatc acattacatg aaggtgatat tgcagagatg aagactggtg
      601 aaggtaaaac attaacttcg atttttccag tatatttaaa tgctttaact ggtaatggtg
      661 ttcacgttgt tactgttaat gattacttag caggacgtga tgctgtaaat aatggcaaag
      721 tatttaactt tttgggatta acagttggat tgaataaacg ggagctaaca cctgaacaaa
      781 aacaagaagc tcatgggtgt gatgttacgt atacaaccaa tgccgaatta ggatttgatt
      841 atttaagaga taacatggta actcgtttag aggataaagt attagtaaaa gggttaaatt
      901 atgcccttgt cgatgaggtc gattcaattt taatcgatga atcaagaaca ccattaatta
      961 tttctggtgg tcgtaaaaat acagcagcgt tatatttaca agctgaccgt tttgttaaat
     1021 ctttgaaaca agataaagat tatgaagttg atatcgaaag taagacggtt gctttaacac
     1081 ctgatggaat tgctaaagct gaaaaaggtt ttaagttgga taacctatat gacccacaac
     1141 atactgcatt agtgcatcat attaatcaag ctttgaaagc taactataca atgactcgcg
     1201 atgttgaata tatggttgct acagaagatg gtactcgtga tattcgtaat gcaaaaatta
     1261 tgattattga ccaatttaca ggtcgtgtta tgcctggtcg tgcttattct gatggattac
     1321 atcaagcaat cgaagctaaa gaaggtgttc caattaaaga agaaacagaa actagagcaa
     1381 caattactta tcaaaatttc tttagattat ttaataaatt agcagggatg acaggaacag
     1441 ctaaaactga agaagaagaa tttagattga tttataatat gcgtgttatt gagattccaa
     1501 ctaaccgtcc agttattcgt gatgatcgca atgataagat ttattctact agagctaata
     1561 aattcaaagc tttatgtgaa gaattcatat ggtcaaccga ttttgattgg gactgtttct
     1621 gttgaaacat ctgaagtatt atcaaaaatg ttagatcgtc gtaaaataag acataacgtt
     1681 ttgaacgcta aaaatcatgc taaagaagct gaaattattg aaaaagctgg acaacgtgga
     1741 gcggtaacga tcgccaccaa catggctggt cgcggtaccg atattaaatt aggtgaaggt
     1801 gttgctgaaa ttggcggttt agcagtaatt ggttcagaac gtcatgaatc tcgccgtatt
     1861 gataatcagt tgcgaggacg ttctggtcgt caaggagatc ctggttattc ggttttctat
     1921 gtatcatttg aagatgattt aatggaacgt ttcgctggag aaagattaaa atcatttaca
     1981 gattatttag aagatgatca agcaattgaa aataaaatgg ttactaaagc aattgaaggt
     2041 gctcaaaaac gtgttgaagg tcaaaacttc gattctcgta aacatattct tgagtatgat
     2101 gatgtaatgc gtcaacaacg tgaaatcatg tataaagagc gtgatgatat tatgtctgaa
     2161 gaaaatttgg atgctatcgt taaaggaatg tttaatcaag caatagaaat gactgtccgc
     2221 caatttacaa aacatgatgg aaaagatgat attgtagatg ttgctggagt tgttgatttt
     2281 gttgctaaaa actatatgtt attagttgaa gtagaagcta gtaactgtga agcattacaa
     2341 aaagatccac aaaaattaat tgaaacatta acggatttag ttttcaatca gtatattagt
     2401 cgttttaata aagagttaga gccagaaaag aaattacaat atgaaagaag tattctttta
     2461 ggagtaattg actatacttg gatcaatcat attgatgcta tgactaaatt acgtaatggt
     2521 atttatttaa gagcttatgc gcaaaaagat cctttagctg aatatacaga agaagctttc
     2581 tacatgtttg aacaaatgac atcaagtatt gcagatgcaa tttctcgtaa tattgtccat
     2641 atgggaatcc gtcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggaac ttacatttaa
     2701 gtaatggaat tatacgaaat taaaaatggg cttactaaag tccatttatt aatgacggag
     2761 ttttatcaat caattgatat tgaagcatat cgtcgagaga ttgaggggct caccgtaatc
     2821 acactacaag agggattttg ggatgatgct aataaagcta aagtgactta tgataagtta
     2881 aataagatga aaaaaacaac tgatcaatat gatttattag aaacgacatt aactagttta
     2941 gatgaaacat atgagcttgt aaagaatact gaggatcaag aatttaagga aattcttgaa
     3001 agtgattata ctgtttttga aaaagaatta tcaaaatttg agacaatgat gttattgtct
     3061 ggtgaacatg acgatttaaa cgcaatcgtt gaaattcatc ctggagcagg tggaacagag
     3121 agtcaagact gggcagaaat gttatttagg atgtatcaac gttatgctgc taataaaggc
     3181 tggaaagtcg aggttttaga ttatttagat ggtgatgtgg caggaataaa atcggttaca
     3241 atgttaataa aaggggataa tgtatatgga catttaaagg cagaaaaggg agttcatcgt
     3301 ttagtgcgct tatcaccttt tgattcagca aaacgccgtc atacttcgtt cgcatcggtt
     3361 gatgtaatgc ccgaatttaa taacgagatt gaaattgaga ttcaaagtac tgacttgaaa
     3421 atagatactt atcgagctag tggagccggt ggtcaacaca tcaacaaaac tgattcagca
     3481 gttcgaatta cacatttacc tactaatatt gttgtaacct gtcaaagcca gcgaagtcaa
     3541 attcaaaacc gtgaacaagc aatggtaatg ttaaaaagca agttatatca gttgatgctg
     3601 gaaaaacaag ctagtgaatt aaaagaatta aaaggagagc aaaaagaaat agcatggggt
     3661 tcacaaatta gatcatatgt acttcatccg tattcgctag tgaaagataa tcgtagtgga
     3721 tatgaatcta ataatcccaa agctgtttta gatggggatc ttgatggatt tatatatgcc
     3781 tatttaaaat cagcattgta aggaggacga catcgtgaag aagcatattt atgaaacact
     3841 gattattatt gtcggtaatt ttattttagc gttaggaatc tgtgctttta ttaccccggt
     3901 tgggttgatt acaggtggtg cttcaggtat cgggattgct gtcaaatcat tgacaggaat
     3961 aaatatttcc tatactgtgt atgcaataaa tattgtaatg tttgttgttg gttat
//
LOCUS       DAOMTA010000198         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_730_length_3984_cov_2.434462, whole genome shotgun sequence.
ACCESSION   DAOMTA010000198 DAOMTA010000000
VERSION     DAOMTA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_3984_cov_2.434462"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLK68_06600"
     CDS             <1..577
                     /locus_tag="PLK68_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRM90114.1"
                     /translation="TKDNRLVLIHDEKIDRTSNGKGYVKDYTYDQLCQFNFNYRFDGI
                     EAKIPLLEELLDYCIGKDVILNIEIKTDKIQYPNIERMTYMMIKEKGLLDRVMFSSFH
                     LESLLNLRELDDSIYLGYLYEDNYQVNKAKVFEYRFNGAHPKYTFLNEKEINDYLRRG
                     IDVNTWTVDSDDIKDFLVDEGVKTIITNKDI"
     gene            767..3415
                     /gene="adhE"
                     /locus_tag="PLK68_06605"
                     /gene_synonym="adhC"
     CDS             767..3415
                     /gene="adhE"
                     /locus_tag="PLK68_06605"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HRM90115.1"
                     /translation="MAEKKAVNKVVEPTNEEIDAHVDQLVAKAQVALSKFEEFTQEQV
                     DYIVAKCSVAGLDAHGTLAEAAVNETGRGVFEDKAVKNLFACEYVTSNLRHLKTVGII
                     NEDPLLGITEIAEPVGVVCGIVPTTNPTSTVIFKSLICLKTRNPIIFSFHPSAHACSV
                     MAAVVIRDAAIAAGAPEDCIQWLDLKSMYATTALMKHPGVATILATGGNAMVAAAYSC
                     GKPALGVGAGNVPAYVEKTCVLPRAVNDIVLSKSFDNGMICASEQAAIVDTEIYDAFM
                     KEIKRFKVYFVNKEEKAKLETFMFGAVAYSDNVNAAKLNPNVVGKPATWIAEQAGFKV
                     PEDTQIICAECKEVGPNEPLTREKLSPVLAVLKAKNTDDGILKATQMVEFNGLGHSAA
                     IHTEDHEISKKFGHACKAIRIIENAPSTFGGIGSVYNAFIPSLTLGCGSYGHNSVSNN
                     VSAVNLINIKRIGRRNNNMQWVKLPPKIYFERNSIKYLRDMKKMEKAMIVTDRGMYNL
                     GYVEKIEDVIRRRRNQVDLELFFDVEPDPSFDTVQAGLELMNKFQPDTIIALGGGSSM
                     DAAKVMWLLYENPEVCFDDIKQKFMDIRKRAFKFPELGKKANLICIPTTSGTGSEVTP
                     FSVITDKSCNKKYPLTDYALTPTVAIVDPEFVMSLPAAIAADTGIDVLTHAVEAYVSI
                     LASDFTDGWAKQAVKLVFDYLEESVKEGTPLSREKMHNAATIAGMAFANAFLGMNHSL
                     AHKIGGEFHVPHGRTNGILLPHVIRYNGSMPTKLNIWPKIENFKADVKYMELAQLIGL
                     NPKTPAEGVQMFADACEELCHKVNLASNIESQGIDKKDWDEAIHRMAMNAYEDQCTPA
                     NPRMPMVHDMEAILRTIWDYKNKFDK"
     gene            3513..>3984
                     /locus_tag="PLK68_06610"
     CDS             3513..>3984
                     /locus_tag="PLK68_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRM90116.1"
                     /translation="MRRNIYNSIYYCVLGLILIVISSMAIIQREDFLMRVFDVLAWIL
                     IINGLHELSVFLRRRFRGDLINIIGNIGVGIFILSYTAIPIRLLFAIFAIYITLNGII
                     KFISYLNYKKDKVSKRFPVLCGALFLIIYGLALLLGRYADANAMMIFIGGYGLLL"
BASE COUNT         1374 a          565 c          747 g         1298 t
ORIGIN      
        1 aacgaaagat aataggctag tcttgattca tgatgaaaag attgatcgga cttctaatgg
       61 taaagggtat gtaaaagatt atacctatga tcaattgtgc caatttaatt ttaactatcg
      121 ttttgatgga atcgaggcaa aaattccgtt gttagaggaa ttgttagatt attgtattgg
      181 taaagatgta atcttaaata ttgaaataaa aactgataag attcagtatc cgaatattga
      241 aagaatgact tatatgatga tcaaagaaaa gggcttatta gatcgagtaa tgttttcatc
      301 gtttcattta gagagtttgc ttaatcttag agaacttgat gatagtattt atcttggcta
      361 tttgtatgaa gataattatc aagtcaataa agctaaagta tttgaatatc gttttaatgg
      421 agcacatcct aaatatactt ttttaaatga aaaggaaatc aatgattatt tgcgtcgggg
      481 tatagatgta aatacttgga cagtagatag tgatgatatt aaagattttt tagttgatga
      541 gggtgttaaa acaataatta cgaataaaga tatctagggt aaaaaggccc tagatttttt
      601 ctcatccagt aaattaagca gtgatacaag tgttagcgtt ttcattgcaa agttagtgtt
      661 tttgttgtga aaaaatgcac aatattactt gtgttttttg caaatttggg gtacactatc
      721 ggtggaaaca aattaaatat ttcacaatga atcggaggaa gaaataatgg cagaaaaaaa
      781 agctgttaac aaagtagttg aaccaactaa tgaagaaatc gatgctcatg ttgatcaact
      841 agttgcaaaa gctcaagtag ctttatcaaa atttgaagaa ttcactcaag aacaagtaga
      901 ttacatcgta gctaaatgtt cagttgctgg acttgatgct catggtacat tagctgaagc
      961 tgcagttaat gaaactggac gtggtgtatt tgaagataaa gcagttaaaa acttatttgc
     1021 ttgtgaatat gtaacaagta acttacgtca cttaaaaact gttggcatca ttaatgaaga
     1081 tccactttta ggtatcactg aaattgctga accagttggt gttgtttgtg gtatcgttcc
     1141 aacaactaac ccaacttcta cagtaatttt caaatcatta atttgtttaa aaacaagaaa
     1201 cccaattatc ttctcattcc atccaagtgc tcatgcatgt tcagtaatgg ctgctgtagt
     1261 aattagagat gctgcaatcg ctgctggggc tccagaagat tgtatccaat ggttagatct
     1321 taaatcaatg tatgcaacta ctgcattaat gaaacaccca ggtgttgcta caatcttagc
     1381 aactggtggt aacgcaatgg tagctgctgc ttactcttgt ggtaaacctg cattaggtgt
     1441 aggagctggt aacgttcctg cttatgtaga aaaaacttgt gtattaccta gagctgtaaa
     1501 tgacatcgta ttatcaaaat ctttcgataa cggtatgatc tgtgcttctg aacaagctgc
     1561 tatcgttgat actgaaatct atgatgcatt catgaaagaa atcaaaagat tcaaagttta
     1621 ctttgtaaat aaagaagaaa aagcaaaatt agaaacattt atgtttggtg ctgtagcata
     1681 cagtgataac gtaaacgctg ctaagttaaa tccaaacgta gttggtaaac ctgctacttg
     1741 gatcgctgaa caagctggat tcaaagtacc agaagatact caaattatct gtgcagaatg
     1801 taaagaagtt ggtccaaatg agccattaac aagagaaaaa ttatctccag tattagctgt
     1861 attaaaagct aaaaatactg atgatggtat cttaaaagca actcaaatgg ttgaattcaa
     1921 tggtttgggt cactctgctg caattcatac agaagatcat gaaatttcta agaaattcgg
     1981 acatgcatgt aaagcaatcc gtattatcga aaacgctcca tctacattcg gtggtattgg
     2041 ttctgtttat aatgcattta ttccatcatt aacattaggt tgtggttctt acggacataa
     2101 ctctgtatca aacaacgtaa gtgctgttaa cttaatcaat attaagagaa tcgggagacg
     2161 taataacaat atgcaatggg ttaaacttcc tccaaaaatt tatttcgaaa gaaattcgat
     2221 taaatattta agagacatga aaaaaatgga aaaagccatg atcgttacag atagaggtat
     2281 gtacaatctt ggttatgtag aaaaaatcga agatgttatt agaagaagaa gaaatcaagt
     2341 tgatttagaa ttattctttg acgtagaacc agatccaagt ttcgatacag ttcaagctgg
     2401 tcttgaatta atgaacaaat tccaacctga tacaattatc gcattaggtg ggggttcttc
     2461 aatggatgct gctaaagtaa tgtggttatt atatgaaaat ccagaagtat gtttcgatga
     2521 tatcaaacaa aaattcatgg atatcagaaa acgtgcattc aaattccctg aattaggtaa
     2581 aaaagctaac ttaatctgta ttcctacaac ttcaggaaca ggttcagaag ttactccatt
     2641 ctcagttatt acagataaat cttgtaacaa gaaatatcca ttaactgact atgctttaac
     2701 tccaacagta gctatcgttg accctgaatt cgttatgtct ttaccagctg caattgctgc
     2761 tgatacaggt atcgacgtat taactcatgc agttgaagct tacgtatcta tcttagcttc
     2821 tgactttact gatggatggg ctaaacaagc cgttaaatta gtatttgatt acttagaaga
     2881 atcagttaaa gaaggaactc ctttatctag agaaaaaatg cacaatgctg caacaatcgc
     2941 aggtatggct ttcgctaatg cattcttagg tatgaaccac tcattagctc ataaaattgg
     3001 tggggaattc cacgtacctc atggacgtac taatggtatc ttattaccac acgttatcag
     3061 atataacgga agtatgccaa ctaaattaaa catctggcca aaaattgaaa acttcaaagc
     3121 tgatgttaaa tatatggaat tagctcaatt aatcggatta aatcctaaaa ctcctgcaga
     3181 aggtgttcaa atgtttgctg acgcttgtga agaattatgt cacaaagtta atttagcatc
     3241 taacatcgaa tctcaaggaa tcgataaaaa agattgggat gaagcaattc acagaatggc
     3301 tatgaatgct tatgaagatc aatgtactcc agcaaatcca agaatgccta tggtacatga
     3361 tatggaagca atcttaagaa ctatttggga ttataaaaat aaattcgata aataataatg
     3421 tttatctttg aggcaacacc aagtgttgcc tcttttttat tagacacttt gtgttataat
     3481 gggcatatat taaagtatcg gggtgtgatt taatgcgacg taatatttat aattcaatat
     3541 attattgtgt tttaggattg attttaattg ttatttcatc aatggcaatt attcagcgag
     3601 aagatttttt aatgcgtgta tttgacgtat tggcatggat tttgattatt aatggtcttc
     3661 atgaattaag tgtattttta agacgtcgct ttagaggaga tttaattaat ataattggaa
     3721 atattggagt aggaatattt attttgtctt atacagcgat tccaattaga ttattatttg
     3781 ctatttttgc aatctatata acattaaatg gaattataaa atttatttct tatttaaact
     3841 ataaaaaaga taaagtgagt aaacgatttc cagttttgtg tggggctctg tttttgatca
     3901 tctatggctt ggcactgtta ttagggagat atgctgatgc taatgcaatg atgatcttca
     3961 ttggcggtta tggcttatta ttag
//
LOCUS       DAOMTA010000199         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_747_length_3941_cov_2.272259, whole genome shotgun sequence.
ACCESSION   DAOMTA010000199 DAOMTA010000000
VERSION     DAOMTA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_3941_cov_2.272259"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1229
                     /locus_tag="PLK68_06615"
     CDS             105..1229
                     /locus_tag="PLK68_06615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM90117.1"
                     /translation="MEKLFIVFDRIPSSTSGGLVTYYMRLANMLKDYYDVVIISIFDT
                     DEVNKKLFEGFNIINISNFNNDLMFVEIVKYIKAYDIINVIKSIYKVIYYFCFIKIAR
                     SKTKKIINTNKCIVSSPSAGIFVSNKIKFILEIHTKYEFFWGDRFISKLQVKLMSKPQ
                     LILFRSKSDKNKAINQYTNVDYIYNFLDNSDINRKQDVFPTNKYLFLGRLSYEKNLFR
                     LLDIAELLKHRNVPFNIDIYGNGPLKQELQENIIQKGLNDKVILKGFCTNKNIYKEYD
                     ALLLTSDIEGYPLVIIEAKANAIPTITTNWGDATSEIIADKENGIICETNDMYVNAVI
                     AMQNPDCLKKMSNAAFDDFINNYSASIVKSKWLDILSREG"
     gene            1233..2117
                     /locus_tag="PLK68_06620"
     CDS             1233..2117
                     /locus_tag="PLK68_06620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM90118.1"
                     /translation="MGNDMLVTVVVMVYKNFSGLEATINSVISQKYQNIELIVSDDGS
                     DNYKELYFSFYEDRCSRRFDRYKLIHNNNNVGTVKHFNNIIKMSNGEIICPLSCGDKF
                     IDQNVVTDIVNYFNNNDCMLCTARRKELGNAERKLPKLYEMKKLNSCRKALNSILLYG
                     NFIGGATTYYKRELFEKYGYFDERYKLCEDLPFFIKMLSNNITISPLNRVTIGYDISG
                     VSSLKNRNKLLDEDYTKIFRNALKDEKKQLFFLTKRALNYRIEKYGTKSNKVALSIKY
                     IDIVIILIFSTILNRWRN"
     gene            2119..2526
                     /locus_tag="PLK68_06625"
     CDS             2119..2526
                     /locus_tag="PLK68_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HRM90119.1"
                     /translation="MEVIKYAFQQHGDDRGQLVSLEELKDIPFQIKRVYYMYDTKMGV
                     RRGFHAHKNLKQILICIHGSCKILLDNGYEKKNVTLETPYEGLYVAHDMWREMYDFSD
                     DAVLMVLASEYYDESDYIRKYEDFLDYVNKNNE"
     gene            2564..3220
                     /locus_tag="PLK68_06630"
     CDS             2564..3220
                     /locus_tag="PLK68_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM90120.1"
                     /translation="MMKVLIVVPAYNEEENIVKTIEDIKRNDCSKDKTYEVCKKNRFN
                     VLHLPINYGLTSGVQVGFKYALENAYDALIQFDGDGQHMAEYIDIMAKELENGYDIVI
                     GSRFVTEKKPYSSRMIGSRLISFLIKVVTGKRINDPTSGMRMFSSRVFNDFANSMNYP
                     PEPDMLACMIKRGYKIREVQVEMRDREFGESYLNPIRSMKYMLEMCVSILFIQSFRKR
                     "
     gene            3226..3564
                     /locus_tag="PLK68_06635"
     CDS             3226..3564
                     /locus_tag="PLK68_06635"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HRM90121.1"
                     /translation="MTTTLQILLIIVSVITFLFVISKIRKSQLNIQDAVIWIMLCLLL
                     IIMSLFLPFITKIAQTLGFMSTSNFVFALISFFLLIIVFSQTVKISILNDKIKNLNHY
                     IAMKEHNEKK"
BASE COUNT         1525 a          396 c          652 g         1368 t
ORIGIN      
        1 tggatttaaa catgaaggaa cttttaaaca acatttatat atgtatagtg aatattacga
       61 tttagaatgg tacgcaatta taagaggaga tcgcacgtaa aatcatggaa aaattattta
      121 tagtatttga tagaataccg tcatctacta gtggtggatt agttacatat tatatgagat
      181 tagcaaatat gttaaaggat tattatgatg tagtgatcat atctatattt gatactgacg
      241 aagttaataa aaaattattt gaaggattta atattataaa tatatcaaat tttaataatg
      301 atttaatgtt tgtagaaatt gttaagtata ttaaagcgta tgatataata aatgtcataa
      361 aatcaatata taaggttata tactattttt gcttcataaa gattgcacgt agcaaaacaa
      421 aaaagataat taatacaaat aagtgtattg tatcatcacc gtctgcgggt atttttgtaa
      481 gtaataaaat taagtttata ttagaaatac atacaaaata tgaatttttt tggggtgata
      541 gatttatttc aaaattacaa gtcaaattaa tgagtaaacc tcaattgata ttattccgca
      601 gtaaatctga taaaaataaa gcgataaatc aatatactaa tgtagactat atatataatt
      661 ttcttgataa ttcagatatt aatcgaaaac aagatgtgtt tcctacaaac aaatatttat
      721 ttcttggaag attgtcttac gagaaaaatt tatttagact tttagatata gcggaattac
      781 ttaaacatcg gaatgtacct tttaacattg atatatacgg aaatggacca ttaaaacagg
      841 agcttcaaga aaatataata caaaaaggat taaatgataa agttattttg aaaggttttt
      901 gcacaaataa aaatatatac aaagaatatg atgccctgct acttacctct gatatagagg
      961 ggtatccatt agtaattata gaagcaaaag caaatgcgat accgacaatt acgactaatt
     1021 ggggtgatgc tacaagtgaa ataatagccg ataaagaaaa cgggataatt tgcgagacga
     1081 atgatatgta tgttaatgcg gttatagcaa tgcaaaatcc ggattgtctg aaaaaaatga
     1141 gtaatgctgc atttgatgat ttcattaata attattcagc ttctatcgtt aaatcaaaat
     1201 ggttagatat actatcaagg gaaggttaat atatgggaaa tgatatgcta gttacagtcg
     1261 tggttatggt ttataaaaat ttttctggct tagaagcaac tatcaactca gttattagtc
     1321 aaaaatatca aaatattgaa ttaatagtaa gtgatgatgg atcagataat tataaagagt
     1381 tgtatttttc tttttatgaa gataggtgtt cgagaagatt tgatagatat aaattgattc
     1441 ataataataa caatgtagga acagtaaaac attttaataa tataattaag atgtctaatg
     1501 gtgaaataat ttgtccatta tcgtgtgggg acaagttcat tgaccaaaat gtggtaactg
     1561 atatagtcaa ctattttaat aataatgact gtatgctctg tacagcaaga agaaaagaac
     1621 ttggtaatgc tgaaagaaag cttcctaaac tttatgagat gaaaaaatta aatagttgta
     1681 gaaaagcatt aaattcaatt ttgctttatg ggaattttat aggaggggca acaacatatt
     1741 ataaacgtga actttttgaa aaatatggat attttgatga aagatataaa ttatgtgaag
     1801 atttaccttt ttttataaaa atgctgtcaa ataatataac tatttcacca ttaaataggg
     1861 ttactatagg atatgatata agtggcgtat cttcattaaa aaatagaaat aaattgttgg
     1921 atgaggatta taccaagatt tttaggaatg ctttgaagga tgaaaaaaaa caattatttt
     1981 ttttaacaaa aagagcttta aattatcgaa tagagaaata cggaactaag tcaaataagg
     2041 tagctttatc aataaaatac atagatatag ttataatttt gattttttca actattttaa
     2101 acagatggag gaattaaaat ggaagtgatt aaatatgcat ttcaacaaca tggtgatgat
     2161 agaggtcaat tagtatcatt agaagaactt aaagatattc cgtttcaaat aaaacgtgta
     2221 tattatatgt acgatacaaa gatgggggta cgtagaggat ttcatgcaca taaaaattta
     2281 aaacaaattt taatttgtat acatggcagt tgtaaaattt tactagataa tgggtatgag
     2341 aaaaaaaatg ttacgctaga aacaccttat gaaggattgt atgtagcaca tgatatgtgg
     2401 agagaaatgt atgattttag tgatgatgct gtattaatgg ttttagcatc tgaatattat
     2461 gatgaaagtg attatataag aaagtatgaa gattttctgg attatgtaaa taaaaataat
     2521 gaataataga agtatttgtt attaaaaata atggagatag tatatgatga aagtattgat
     2581 agttgttcca gcctacaatg aagaagaaaa tattgtaaaa acaattgaag atattaagcg
     2641 taatgattgt tctaaagata agacatatga agtctgtaaa aaaaatagat ttaatgtttt
     2701 acatctacct ataaattatg ggctgacaag tggagtacaa gtaggattta aatatgcctt
     2761 agaaaatgct tatgatgcac ttattcaatt tgacggagat ggtcaacata tggccgaata
     2821 tatagatatt atggcaaaag agttagaaaa tggatatgat atagttattg gttctaggtt
     2881 tgttactgaa aaaaaaccat attcatctag aatgatagga agccgattga tttcattttt
     2941 aattaaagtt gtcacaggaa aaagaattaa cgatcctact tctgggatga gaatgttctc
     3001 tagtcgggta tttaatgatt ttgcaaatag tatgaattat ccaccagaac cagatatgct
     3061 tgcatgtatg attaaaagag gatataagat tagagaggtt caggttgaaa tgagggatcg
     3121 cgagtttggg gaaagttatt taaatccaat aagatctatg aaatatatgc tagaaatgtg
     3181 tgtatccata ttatttattc aatcgtttag aaagaggtaa taaaaatgac aacaacttta
     3241 caaattttat taataatagt atcagtaatt actttccttt ttgtcatatc taaaataagg
     3301 aaatctcaat tgaatataca agatgcagtt atttggatta tgctgtgctt gttattgata
     3361 attatgagtt tgttcttgcc attcataacc aaaatagcac agactttggg atttatgagc
     3421 acttcaaatt ttgtatttgc attaattagt ttcttcttat taattattgt gttttctcaa
     3481 acagtaaaga tttcaatatt gaatgataaa attaaaaatt taaatcatta tattgctatg
     3541 aaagagcaca atgaaaaaaa atgaatataa ttacttaaaa aatccatatg ataggttggc
     3601 gctttaaaat aatgtagggt tggaagatgt aaagtcttca tcaataaccc tattttttat
     3661 gtatctagat atactatcaa atttatattt tatttagatc tgttttttct taatttgtat
     3721 ttcattaata gaaattgtat atgtatctaa aaagttttgt gaatacataa ttctatttct
     3781 atcaaaatta gtcattttat ttgtatctgt taactctttt tatatagtta tttttccttc
     3841 aatacattcg ttagatattc ttcttccatc aatccattta aaagagttca tacttttctc
     3901 catttcccca cgctaagcgc atggacacaa atatttcttg a
//
LOCUS       DAOMTA010000200         3909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_762_length_3909_cov_2.497405, whole genome shotgun sequence.
ACCESSION   DAOMTA010000200 DAOMTA010000000
VERSION     DAOMTA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_762_length_3909_cov_2.497405"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..686
                     /locus_tag="PLK68_06640"
     CDS             465..686
                     /locus_tag="PLK68_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90122.1"
                     /translation="MQAHTKLENYCEEFDSIKLDTMLTGKRIKATRKQKNITQEKLAD
                     ICGCTPTHISNIENGKIGISCGSICIAFG"
     gene            673..1047
                     /locus_tag="PLK68_06645"
     CDS             673..1047
                     /locus_tag="PLK68_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90123.1"
                     /translation="MHSVDSYTYECTSIPAQTISLEFFENEIWYIRLILEGEDRIAKN
                     MMKQEQLKTISYSAYVEDWEVLRCVDGCLEQIIMRENIEMMLSALTDKQREINTRSFL
                     KIEISRKPVNCWGLHSRRYLSL"
     gene            complement(1433..>1643)
                     /locus_tag="PLK68_06650"
     CDS             complement(1433..>1643)
                     /locus_tag="PLK68_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM90124.1"
                     /translation="ERPIFILLNQQIIDYTKKADCKDITWDILEFSRGRIYNACISFK
                     ELDNTWYKQQINKTISYLKYLTLKT"
     assembly_gap    1644..1653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1654..1992)
                     /locus_tag="PLK68_06655"
     CDS             complement(<1654..1992)
                     /locus_tag="PLK68_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM90125.1"
                     /translation="MNNIKTSKAENTKTNIIKALEQLSKDQNLDNIKIRDICTLANIS
                     VGTFYLYFKSKEEAILYIYQQCDEQFRNLKLTNDPLDNIYQILDTYYHMVDIDNINFD
                     RTIYKCHLNHY"
     gene            2097..>3909
                     /locus_tag="PLK68_06660"
     CDS             2097..>3909
                     /locus_tag="PLK68_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM90126.1"
                     /translation="MKSIYEPLFEPLKIGNVEVKNKFFMAPMATIVDVDEDYCYTQQS
                     IDYYVERAKGGVGLIITGANWVENDVEPHARCSFPCPNMLPLKYEHKAKEMTDRTHAF
                     GTKIFLQLTAGLGRSALPNFVDMKDFVAPSPTTNRWIPNAPCRELTTEEIEHIIEKFG
                     DAAVIAKNSGFDGVEIHAVHEGYLLDCFTMTLFNQRTDKYGGDLKGRLRFATEIVETI
                     KNKCGKDFPVILRFSIKSYIKQLRQGGLPGEDFKELGRDIDEAIEAVKILQEAGYDAF
                     DADAGTYDSWYWAHPPIYFEKGMYLPLVEKIRDAAKVPLLIAGRMDDPKMAVDALNKG
                     TIDGVGLGRPLLSDPDYVNKLKDGLEALIRPCLGCHDGCFGRLLQSGLGSCAVNPECG
                     REDTVGVAPAFVKKNVVIVGGGPAGLEAARVSALRGHKVTLFEAADRLGGELLVGGMP
                     KFKEDDLALASYYTNELERLGVDVKLNTLADEAAIDALKPDSLFICEGSIPVIPPIDG
                     VENAVLANEVLTNKVAAKDNVVVIGGGLVGCELALHLAQNGKKVTIIEALPDILKSGI
                     ALPPMNEWMLRDLLAFNNVRIVGNAKVSKIGNQEIKVL"
BASE COUNT         1333 a          498 c          777 g         1291 t
ORIGIN      
        1 tgcagataca atctgctttg ctaacattga taatgatagt tttttcattt taatttcctt
       61 tcaaataaat gtcgtataaa atgacattga aaaacgctac attgttatta aaaacagtat
      121 tataatacta cgaactttat ataaatgcaa taaggtagtg gtatttaatt taatatacat
      181 tataatgtcg ttttgcacga cattataata tttagaaaag tgattttaaa taacattagc
      241 acacatcaat aaaatatacg gtttgtcaaa tgtattttcg acaataccag acaagataat
      301 gcttagaaag tgaattgaaa cataaaaata tatgtctggg ttcaatattt ttcgacaaaa
      361 tatgatgtgg tacgacaagt tattagttcg tagacaattc aaatgatgga ggtgttcaag
      421 tataatagca acgtaccaaa tcgaaccgaa tggaggtgga gaacatgcag gcacatacga
      481 aattggaaaa ttactgtgaa gaatttgata gtattaaact ggatacgatg ttaactggca
      541 agcgtataaa agctaccagg aagcaaaaga atatcacaca ggaaaaattg gctgatattt
      601 gcggatgtac acctacgcat atctctaata ttgaaaatgg taagatcggg atttcatgcg
      661 gtagtatttg cattgcattc ggttgattca tatacatatg aatgtacttc aatcccggct
      721 cagacaatta gtttggagtt ttttgaaaat gaaatatggt acattcggtt aattctggag
      781 ggagaagatc gcattgctaa aaacatgatg aagcaggagc aattaaaaac aatctcatat
      841 agtgcctatg tagaggattg ggaggtgctt cgctgtgtgg acggatgttt agaacaaatt
      901 attatgcgtg aaaatataga aatgatgtta tctgcattga ctgataaaca aagggagatt
      961 aatacaagaa gttttttgaa gatagaaatc agcagaaaac cagtgaattg ttggggatta
     1021 cacagcaggc ggtatctgag tctatgagac aatcctttga taaattacgt aggctttttg
     1081 atatgaaaga atttatttaa aaaatatgaa ttggtacttg tatttgaggg actgagcaag
     1141 ggtatggtaa taataggtcc aatttatgca gagtaatttc atatctggct tataaaaaaa
     1201 gaattccgtg ttatatcgtt tcaccaagtg atgatagagg ggatagaatt gatacattta
     1261 atagtttttt agttaatcta actaaagcgc attatgtatt ttgtaataaa aataatattg
     1321 cagatacagt agagaaatca atattagact taacttcaaa gggttataaa ccatattata
     1381 taaatggaga taaatacggt aaaggacaga atgatttaaa tcattctgtc ctttatgttt
     1441 taagtgttag atattttaaa taagagattg ttttatttat ttgttgttta taccaggtat
     1501 tatctaactc cttaaaagaa atacaggcat tataaatacg cccacgagaa aattctagaa
     1561 tatcccaagt tatatcttta caatcagctt tttttgtata atcaataatc tgttggttta
     1621 gtaaaataaa gattggccgt tctnnnnnnn nnnataatga tttaggtggc atttataaat
     1681 agtacgatca aaatttatgt tatcaatgtc aaccatatga taataggtat ctaagatctg
     1741 atagatatta tcaagtggat cattagttag ttttaaattt ctaaactgct catcacattg
     1801 ttgataaata tataaaatag cttcctcttt acttttaaaa tataaataaa aggttcctac
     1861 tgagatattt gctaatgtac atatatccct tatttttata ttgtcaagat tttgatcctt
     1921 agataactgt tccaaagcct taataatatt agttttagtg ttttctgcct tacttgtttt
     1981 aatgttattc ataattaatc ctttcattat acattttatc ttaaaaacat tgtaagcgca
     2041 aacaatgagt gttaaaatat aaatatgaac aagttcagtt ttgaaaggag actactatga
     2101 aaagtattta tgaaccatta tttgaaccgc taaaaattgg gaatgttgag gtgaaaaata
     2161 agttttttat ggcaccgatg gcaacaatcg tagatgttga tgaagattat tgttataccc
     2221 aacaaagtat tgattattat gttgaaagag caaaaggtgg cgtaggtttg attataacag
     2281 gagctaactg ggttgaaaat gacgtggaac ctcatgctcg ctgcagtttt ccttgcccaa
     2341 atatgttacc tttaaaatac gaacataagg ctaaggaaat gactgatcgt actcatgctt
     2401 ttggaacaaa gatattttta caattaactg ctggtttagg tagatcggcg ctgcctaatt
     2461 tcgttgatat gaaagatttt gttgctccaa gtccaacaac aaatcgttgg attcctaatg
     2521 caccttgtcg tgaactaacg accgaagaaa ttgaacatat tattgagaag ttcggtgatg
     2581 cagcagttat tgctaaaaat tcaggttttg atggggtcga aatccatgct gtccatgaag
     2641 gatacttatt ggactgtttt acaatgactt tgtttaacca aagaaccgat aaatacggtg
     2701 gagatttaaa aggacgctta cgctttgcta cagaaattgt tgaaacaatt aaaaataaat
     2761 gtggtaaaga ttttccagtt attttgcgtt tttcaattaa gagctatatt aaacaattac
     2821 ggcaaggtgg cttaccaggt gaagatttta aagagttagg aagagatatt gatgaggcta
     2881 ttgaagctgt aaaaatattg caagaagctg gttatgatgc ttttgatgca gatgctggta
     2941 cttatgattc ttggtattgg gctcatccac caatatattt tgaaaaaggt atgtatttac
     3001 cattagttga aaaaattcgt gatgctgcaa aagtaccatt attgattgcc ggaagaatgg
     3061 atgatccaaa aatggctgtt gatgctctaa acaaaggaac tattgatgga gttggattgg
     3121 gacgtccttt attaagtgat cctgattatg taaataaatt aaaagatgga ttagaggcat
     3181 taattcgccc atgtttagga tgtcatgatg gctgctttgg gcgtttatta caatctgggt
     3241 taggtagttg tgcggttaac cctgaatgtg gacgagaaga tactgtgggt gtcgcaccag
     3301 catttgtaaa gaaaaatgtt gttattgttg gtggcggacc agcaggatta gaagcagctc
     3361 gtgtatctgc tcttcgtggt cataaagtta ctttatttga agcagcagat cgccttggtg
     3421 gtgaattatt agttggtggt atgcctaagt ttaaagaaga tgatttagcg ctagcttctt
     3481 attatacaaa tgaattagaa agattaggtg tagatgttaa actaaatact ttagctgatg
     3541 aggcggcgat tgatgcatta aaaccggata gtttatttat ctgtgaaggt tctattccag
     3601 tgatacctcc aattgatggt gttgaaaatg cggtactagc taatgaagta ttgactaata
     3661 aagtagctgc aaaagataac gtagtagtta ttggtggtgg tcttgttgga tgtgaattag
     3721 cgttacattt ggcacaaaat ggtaagaagg taacaattat cgaagcctta ccagatattt
     3781 taaaatcagg tattgcttta ccgccaatga atgaatggat gcttcgtgat ctattagcat
     3841 ttaataatgt tcgaattgtg ggaaatgcaa aagttagtaa aatcggtaat caagaaatta
     3901 aagttcttc
//
LOCUS       DAOMTA010000201         3905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_764_length_3905_cov_1.985195, whole genome shotgun sequence.
ACCESSION   DAOMTA010000201 DAOMTA010000000
VERSION     DAOMTA010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_3905_cov_1.985195"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>618
                     /locus_tag="PLK68_06665"
     CDS             2..>618
                     /locus_tag="PLK68_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM90127.1"
                     /translation="MKKLLASLVVFSFLLVGCGGSSSEEFEVALVTDAGDINDKSFNQ
                     SAWEAVEQYQKDHDATIKYYKPASFDDAGYKSQIEEAVENGAKIIVCPGFKFCNAIGE
                     LQDKYKDVKFILIDATPTVPDEKDSTKSNPVDPGDNVYCALYTETQPGYLAGYAAVKA
                     GHTKLGFMGGIALPAVINYGYGYIQGANDAAKELGVTVDMMYTYTG"
     assembly_gap    619..630
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <631..1105
                     /locus_tag="PLK68_06670"
     CDS             <631..1105
                     /locus_tag="PLK68_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM90128.1"
                     /translation="TFNEDPAIKTKAAKWYDAGTEAIFSCGGGICNSIFPAAKEAGKY
                     AIGVDSNQNDDKTGVVITSALKDVKKTVYDKLVDYKNDKFKGGIETLDASGGYVGLPD
                     DFDRLGDFNAAAYKEVFDKVASGEIKIKSMTDITDNDNGNPTKVPVTNVNITYDK"
     gene            1192..2724
                     /locus_tag="PLK68_06675"
     CDS             1192..2724
                     /locus_tag="PLK68_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90129.1"
                     /translation="MEYVIEMLNITKEFPGIKANDNITVQLKKGEIHALLGENGAGKS
                     TLMSILFGLYQPDEGCIKINGEIAEINDPNDANRYKIGMVHQHFKLVETFTVLDNIIL
                     GVEETKNGLLAKDNARKKVLDLSKQYHLNIEPDALIKDITVGMQQRVEILKMLFRDNE
                     ILIFDEPTAVLTPQEIEELLKIMKGLVAEGKSIIFITHKLNEIKAVADRCTVIRKGKY
                     VATVDVTTTSKEELSKMMVGRDVNFIVEKGKATPGQTVLKVENLNYCKKNAMKNALTD
                     INFEVKRGEIVCIAGIDGNGQSELVYALTGMLTGYTGKIILNNEDITHYSVRQRNLKG
                     ISHIPEDRHKHGLVLDYNLAENMILKNYFNKDNQKHGFLKFENIYNYADRLIEKFDVR
                     SGQGSHSIVRGMSGGNQQKAIIAREIESNCDLLIAVQPTRGLDVGAIEFIHKQLIQQR
                     DEGHAVLLISLELDEVMNVSDRILVMYEGEIVTDLNPKETNVEELGLYMAGSKRGVGY
                     EK"
     gene            2714..3808
                     /locus_tag="PLK68_06680"
     CDS             2714..3808
                     /locus_tag="PLK68_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90130.1"
                     /translation="MKNKAGTKSFLSSLIAILCGLIFGLLIMFLVIPNQALEGFSILL
                     RGGFYRGIKSVGEVLYLSVPIMMTGLSVAFAFKCGLFNIGTPGQYIVGAFASMFIAFN
                     ATFIPESIRWIFAVLAGGLAGALWASVPGLLKAYRNVNVVISSIMMNYIGMLLVIEGV
                     KKFMYNPTGAESYTIPTSLAIPNLGLDQVFNGSSINLGTFIAIGVCILAYVIMNKTTF
                     GYELKATGYNPDASKYAGMNEKKSIVLSMVIAGFFAGLGGALVYMAGTGRTIGTAEVL
                     AAEGFNGIPVALLGFNNPLGVIFAALFIAYINLGGNYVQAVHIAVEIIDVIVAAIIYF
                     SSFTLFIRLILERRKGRKKDKKQALIGGDK"
BASE COUNT         1327 a          494 c          813 g         1259 t
ORIGIN      
        1 tatgaaaaag cttttggctt cacttgttgt attctcattt ctattagtgg gatgcggtgg
       61 tagtagcagt gaagaatttg aagtagcact tgtaacagat gcaggggata ttaacgataa
      121 atcgtttaat cagtctgcat gggaagctgt tgaacagtat caaaaagatc atgatgcaac
      181 tatcaaatac tataaaccag caagttttga tgatgctggc tacaaatcac aaattgaaga
      241 agctgtagaa aatggggcaa aaattattgt ttgtccaggt tttaaattct gtaatgcaat
      301 tggtgaatta caagataaat acaaagatgt caaatttatc ttgattgatg ctacaccaac
      361 agttccagat gagaaggata gtactaaatc taatccagtt gatcctggtg ataatgttta
      421 ttgtgcattg tatacagaaa cacaacctgg atatttagca gggtatgcag ctgtcaaagc
      481 aggtcacact aaacttggat ttatgggtgg aattgcatta ccagctgtaa ttaattatgg
      541 ttatggatat attcaaggag caaatgatgc agcaaaagaa ttaggtgtta cagtcgatat
      601 gatgtataca tatacaggnn nnnnnnnnnn aacatttaat gaagatcctg cgattaaaac
      661 taaagctgct aagtggtatg atgctggaac tgaagctatt ttctcatgcg gtggtggaat
      721 ttgtaactct attttccctg cagcaaaaga agctggcaaa tatgcaattg gtgttgatag
      781 caaccaaaat gatgacaaga caggtgttgt tattacttca gcattaaaag atgtaaaaaa
      841 gacagtttat gataaattag tcgattacaa aaatgataaa tttaaaggtg gtatcgaaac
      901 attagatgca tcaggtggtt atgttggttt accagatgac tttgatcgtt tgggtgattt
      961 taatgctgca gcatacaagg aagtatttga taaagtggca agtggtgaaa ttaaaatcaa
     1021 atcaatgaca gatattactg acaatgataa tggtaatcct actaaagtac ctgtcactaa
     1081 tgtaaatatt acatatgata aataataaaa agcgagacaa ctcgcttttt ttaaaagtat
     1141 ttctcaatct aaccgagaaa aatcaacatt agataagaaa ggagtgacgg tatggaatat
     1201 gtaatagaga tgttgaatat cactaaagaa tttcctggaa ttaaggctaa tgataatata
     1261 accgtacaac taaaaaaagg tgagattcat gctttgttag gagaaaatgg agcaggaaaa
     1321 tcaacattga tgtcaatcct attcgggctg tatcaacctg acgagggatg cattaaaatt
     1381 aacggtgaaa ttgctgaaat aaatgatcct aacgatgcta atcgttataa aattggaatg
     1441 gttcatcaac attttaaatt agtcgaaaca tttacagttt tggataatat tattttgggt
     1501 gttgaagaaa ctaaaaatgg attattagcc aaagacaatg ctcgtaaaaa ggttcttgat
     1561 ctttctaaac aatatcattt aaatatcgag cctgatgctc ttataaaaga tataacagta
     1621 gggatgcaac agcgagttga aattttaaaa atgctcttta gggataatga gattttaata
     1681 tttgatgaac caactgcagt tttaacccct caagaaattg aagaattatt gaaaattatg
     1741 aaaggattag ttgccgaagg aaaatcaatt atttttatta cacataaatt aaatgaaatc
     1801 aaagcggtag ctgatcgatg tactgttatt agaaaaggaa agtacgttgc tactgttgat
     1861 gttacaacga cctcaaaaga agaattgtca aagatgatgg taggccgtga tgttaatttt
     1921 attgttgaaa aaggaaaagc aactcctggg caaacggttt taaaggttga aaatctcaat
     1981 tattgtaaga aaaacgctat gaaaaatgcc ttgacagata ttaattttga agttaaacgc
     2041 ggtgaaatag tctgtattgc aggaattgat ggaaacgggc aaagtgaact tgtatatgca
     2101 ttaactggaa tgttaactgg atatacaggt aagataattt taaataatga ggatataacc
     2161 cattattcgg ttaggcaaag aaatttaaaa ggaatttccc atattcctga agatcgtcat
     2221 aagcatggac tagtactaga ttataattta gcagaaaata tgattttaaa aaattatttt
     2281 aataaagata accagaaaca tggtttctta aaatttgaga acatttataa ttatgcagat
     2341 agattgattg aaaaatttga tgttcgttct ggtcaagggt cacattcgat tgttcgagga
     2401 atgtctggtg gaaatcaaca aaaagcaatt attgcgcgag aaattgaaag taattgtgat
     2461 ttattgattg ctgttcaacc tactcgtggg cttgatgttg gagcaataga gtttattcat
     2521 aaacagttga ttcaacagcg cgatgaggga catgcggttt tactaattag tttagaactt
     2581 gatgaagtta tgaatgtcag tgatcgaatt ctggtaatgt atgaaggaga gattgttact
     2641 gacttaaatc caaaagaaac taacgttgaa gaattaggat tgtatatggc gggatcaaag
     2701 cgaggtgttg gatatgaaaa ataaagcagg gacaaaatca tttttatctt ctttgattgc
     2761 gattttatgc ggtttgattt ttggactact aattatgttt ttagtaattc ctaatcaggc
     2821 attagaagga ttttcaatct tacttcgagg tggtttttat cgtggaatta aaagcgttgg
     2881 tgaagtatta tatttatcag taccaattat gatgaccggt ctttcagtgg cttttgcatt
     2941 taaatgtggt ctatttaata ttggaacacc gggacaatat attgtagggg cctttgcttc
     3001 aatgtttata gcttttaatg caacttttat ccccgaatct attcgttgga tctttgctgt
     3061 gcttgcagga ggattagcag gagccttatg ggccagtgtt cctggattac ttaaagctta
     3121 tcgcaatgtt aatgttgtaa tatctagtat catgatgaat tatattggga tgctattagt
     3181 aattgaagga gtaaaaaaat ttatgtataa tcccactggc gctgaatcgt atactattcc
     3241 tacaagttta gcaataccaa atttaggatt agatcaggtt tttaatggtt cgagcattaa
     3301 tttaggaacg ttcattgcta ttggtgtatg tattcttgct tatgttatta tgaataaaac
     3361 aacatttggt tatgaactaa aagcaactgg atataatcca gatgcgagta aatacgcggg
     3421 aatgaatgag aaaaaaagta ttgttttatc gatggtaatt gccggttttt ttgcaggact
     3481 aggtggagct ttggtttata tggcgggaac tggacgaaca ataggaacag cagaagtttt
     3541 agccgctgaa ggttttaatg gtattcctgt tgccttatta gggtttaaca atccacttgg
     3601 cgtgattttc gctgcattat ttatcgcata tatcaattta ggcggaaact atgttcaagc
     3661 agtacatatt gcagtagaaa ttatcgatgt aattgttgca gcaattattt attttagttc
     3721 tttcacattg tttattcgat taattttaga acgccgaaag gggcgtaaaa aagataaaaa
     3781 acaagctttg attggagggg ataaataatg gatttatttt atttcttatt tagacaaagc
     3841 ttgatgtttt cgatccctct gttgatcgtt gctttaggcg ggttgtttag tgaacgctct
     3901 ggagt
//
LOCUS       DAOMTA010000202         3887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_777_length_3887_cov_2.177975, whole genome shotgun sequence.
ACCESSION   DAOMTA010000202 DAOMTA010000000
VERSION     DAOMTA010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_777_length_3887_cov_2.177975"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1662)
                     /locus_tag="PLK68_06685"
     CDS             complement(238..1662)
                     /locus_tag="PLK68_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRM90131.1"
                     /translation="MKKIKNLTIGNKIVIVSVLLVIIVGGVATYFLTRSPIVFKADII
                     KVEINDSYDVMKNIASVRNGNIKDVKADISKIDYNKLGRYPVIYIYKDKKYEATIEIV
                     DTKKPQFDVVDLDIDLGMKVDSASMATNINDATKTEVSFKEDYDFSKEGTVEVIIQVK
                     DKGNNVTEKKGKVKVTKDSEPPQITGLEPLTIVIGAKTDYNSGVSISDNRDPEPKLTV
                     DSSNVNIQKEGTYTLIYIGIDRSGNKIEKQREIKVVEKKAIGSNNQTSDKIVYLTFDD
                     GPSANTQKILDILDVYGAKATFFVTGNNKPYNHLIKTAHDKGHTIALHTYSHDYKTVY
                     ASPEAYFDDLTKVGNMVKDIIGFVPKYVRFPGGSSNTVSRKYCPGIMTVLSRELINRG
                     YQYYDWNGDSTDASGNNVAVSKLIANATSSKANNINILFHDTAAKSTTVQALPAIIEN
                     YKARGYRFEAITDSSFVPHQGINN"
     gene            complement(1804..2967)
                     /locus_tag="PLK68_06690"
     CDS             complement(1804..2967)
                     /locus_tag="PLK68_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRM90132.1"
                     /translation="MYNIKLLNKISKVGLDGFDENYAYSEEMTNEDAILVRSASLHEY
                     DFGENLKAIARAGAGVNNIPLDDCSEKGIVVFNTPGANANAVKELVLCGLFLSSRKIV
                     ESIRWIDGLKDDQDIAKTAEKGKSNFVGPEIEGKTLGVIGLGAIGVNVANAAIKLGMK
                     VKGYDPYIGVNAAWALSKHARHAASLEEIYQECDYITIHVPSTKETKGFMNKEAFAQM
                     KTGVRILNFARGDLVNNEDLLANVASGKINKYISDFAAPELIGQENIIILPHLGASTP
                     ESEDNCAKMAVEEVCEYLENGNIINSVNFPGVNQARMSKTRLCIINKNVPNILANISK
                     LFADHNLNIENMVNRSRGEYAYTLIDTNDEVRPDIIERIESANGIINVRAIID"
     gene            complement(2978..>3887)
                     /gene="serC"
                     /locus_tag="PLK68_06695"
     CDS             complement(2978..>3887)
                     /gene="serC"
                     /locus_tag="PLK68_06695"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRM90133.1"
                     /translation="KKVMNIPDDYKIVFIQGGATQQFSMVPLNLLKNGKADYVVTGAF
                     SKKAAAEAKKFGEINIAYDGSENNFKHIPTQDELKLDPEASYVHLCANNTIYGTEWKY
                     IPETNGVPVIADMSSNILSKPVDVSKFGMIYAGAQKNMGIAGLGVAIIKEDLLQKVAA
                     TTPVLLDYKLMIENDSMYNTPPAYAIYVLGLVLEWIDSMGGLEVMQERNIKKANLLYD
                     YLDSSDFYIAHSDKDNRSLMNVTFTTPNKDLDAKFVKESIAAGMTNLKGHRSVGGIRA
                     SIYNAMSLEGVEKLVAFMRDFEAANK"
BASE COUNT         1248 a          739 c          455 g         1445 t
ORIGIN      
        1 ataagaataa tttcattatg attttcgtca agtgagctta caatattatt attaataatt
       61 ttatgaatta tcataagaca cctccttttt tgcatagaaa aagccaaact caaataatat
      121 atataaaaaa aatatatatc catcaaagtt tagcctaatt gaatagtaac aatctttgta
      181 attttatgat agcacttcca aaattaattg tcaattacca atcatatata tcaaaaatta
      241 gttgttaatt ccttgatgcg gcacaaacga actatctgta attgcctcaa agcgataacc
      301 acgtgcttta taattttcaa ttattgccgg tagagcctga acagttgttg atttggcagc
      361 tgtatcatga aataaaatat taatgttatt tgctttgctt gatgtagcat tagcaattaa
      421 cttgctgact gctacattat ttcctgaagc atctgtagaa tcgccattcc aatcataata
      481 ttgataaccg cgattgatta attcccttga caatacagtc ataatacctg gacaatattt
      541 acgtgatacc gtatttgaag aacctccagg aaagcgtaca tatttgggta caaacccaat
      601 tatatccttc accatattac cgacttttgt taaatcgtca aaataagctt ctggtgaagc
      661 ataaacagtt ttatagtcat gactatatgt atgcaacgct attgtatgtc ctttatcatg
      721 agcagtttta attaaatgat tataaggttt attattccca gtaacaaaga atgtcgcctt
      781 tgctccataa acatctaaaa tatctaaaat cttttgggta ttagctgatg gaccatcatc
      841 aaaagttaaa taaacgattt tatcacttgt ttgattattg cttccaattg ctttcttttc
      901 aactacttta atttcacgct gcttttctat tttgtttcca gaacgatcta tccctatata
      961 aataagtgta taagtcccct ctttttgaat attcacatta ctagaatcaa ccgttaattt
     1021 tggttcggga tcacggttat cactaatgct gacaccacta ttataatctg tttttgcccc
     1081 tatcacgatc gtcaaaggct ctaaccctgt aatttggggt ggctcactat ctttagtaac
     1141 cttgactttt cccttctttt cagttacatt atttccttta tccttaacct gaataattac
     1201 ttcaacagtt ccctctttac taaaatcata atcttcttta aaagaaactt cagtttttgt
     1261 tgcatcatta atatttgttg ccatacttgc agaatcaact ttcattccta aatcaatatc
     1321 aagatcaaca acatcaaact gtggtttttt agtatcaact atctcgatcg ttgcttcata
     1381 ttttttatct ttatatatat agattactgg atatcttccc aacttattat aatctatttt
     1441 agaaatatca gcctttacat ctttaatatt tccattgcgt acgcttgcga tatttttcat
     1501 cacatcataa ctatcattaa tctcgacttt gataatatcc gctttaaaga ctatcggtga
     1561 gcgggttaaa aaatatgttg ctactccacc aactattatg actagtaata ctgatacaat
     1621 tacaatctta tttcctatag ttaaattttt aatttttttc attatatctt tcccttaact
     1681 aatttatata cttttagtat atgtcaaacc tcagtatttt acaatctttg tcaataaaac
     1741 tatttataaa aatcaactaa ataaaaaata cttatctata atcagataag tatttaatca
     1801 ttattaatca ataatagcac ggacattaat aattccattt gccgactcaa tccgctcaat
     1861 aatatcaggt cgaacttcat catttgtatc aattagtgta tatgcatatt caccacgaga
     1921 gcgattaacc atattttcaa tattcaaatt atgatcagca aataatttag aaatatttgc
     1981 taaaatatta ggaacatttt tattaataat acataatctt gttttagaca ttcttgcttg
     2041 attgactcct gggaagttta ctgaattaat gatatttccg ttttctaaat attcacaaac
     2101 ttcttcaacc gccatctttg cacaattatc ttctgattct ggtgtacttg cacccaaatg
     2161 tggaagaata ataatatttt cttgtccaat taattctggt gcagcgaaat cactaatata
     2221 tttattaatt ttaccgcttg caacattagc taataaatct tcattattaa ctaaatcacc
     2281 acgagcaaaa tttaaaattc tgacaccggt tttcatctgt gcaaatgctt ctttgttcat
     2341 gaaacctttt gtttctttag tactaggtac atggattgta atatagtcac attcttgata
     2401 aatttcttct aaagaagctg catgacgagc atgcttagat aaagcccagg ccgcatttac
     2461 accaatatac ggatcataac cttttacttt cattcctaat ttaatagctg cattggcaac
     2521 atttactcca atagcaccta agccaattac ccctaaagtt tttccttcaa tttctggtcc
     2581 aacaaaatta cttttacctt tttctgctgt tttagcaata tcctgatcgt cttttagacc
     2641 atcaatccaa cgaatacttt caacaatttt tcttgaagag agaaataatc cacataatac
     2701 aagttcttta acagcgttag catttgctcc cggtgtatta aatacaacaa ttcctttttc
     2761 actacaatca tcaagtggaa tattatttac tcctgctcct gctcttgcaa tagcttttag
     2821 attttcccca aaatcatatt catgtaaaga agctgatcta accaaaatag catcttcatt
     2881 agtcatttct tcgctataag cataattttc atcaaatcca tctaatccta ctttagatat
     2941 tttatttaat aattttatgt tatacataat tattctccta tttgttcgct gcttcaaaat
     3001 ctctcatgaa agcaactaat ttttcaacac cttctaatga catagcatta tagatagatg
     3061 ctctaattcc cccaactgaa cggtgacctt ttaaattagt catacccgcg gcaatacttt
     3121 ccttaacaaa cttagcatca agatctttgt ttggtgtagt aaaagtaaca ttcattaaag
     3181 aacgattatc tttatcacta tgtgcgatat agaaatcact actatcaaga taatcataaa
     3241 gaagattagc ttttttaata tttctttctt gcataacttc aagtccaccc atactatcga
     3301 tccattccaa aactagtcct aatacataaa tcgcataagc cggcggagta ttatacattg
     3361 aatcattttc aatcatcaat ttataatcta ataatactgg tgttgtcgca gcaacttttt
     3421 gtaataaatc ttctttaata attgctactc ctaatccagc gatccccata tttttttgtg
     3481 cacctgcata aatcattcca aactttgaaa catccactgg ttttgataaa atatttgatg
     3541 acatatccgc aattacagga acaccattag tttctggaat atatttccat tcagttccgt
     3601 aaattgtatt atttgcacat aaatgaacat aagatgcttc aggatctaat ttcaattcgt
     3661 cttgagttgg aatatgttta aaattgtttt cacttccgtc ataagcaata ttaatttccc
     3721 caaatttttt tgcttcggct gcagcttttt tactaaaagc tccagttaca acataatcag
     3781 ctttaccatt ttttaataag tttaatggaa ccattgaaaa ttgttgtgtt gctccccctt
     3841 ggataaacac aattttgtaa tcatcaggga tattcattac tttcttt
//
LOCUS       DAOMTA010000203         3876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_782_length_3876_cov_2.422664, whole genome shotgun sequence.
ACCESSION   DAOMTA010000203 DAOMTA010000000
VERSION     DAOMTA010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_3876_cov_2.422664"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLK68_06700"
     CDS             <1..183
                     /locus_tag="PLK68_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HRM90134.1"
                     /translation="VYVYKREYDGKKLFVLSNFTAKEVTYDKTLFEAEAKVLLGNYND
                     LIRGKLRAYEAVVLVV"
     gene            227..1366
                     /locus_tag="PLK68_06705"
     CDS             227..1366
                     /locus_tag="PLK68_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90135.1"
                     /translation="MPKKYFKIGCTVIIVTLIGLFLKNNAPANNYKNPDTLNIQDKQS
                     MMMADKAVEKIARGTSGKFETLYLGADGQFQWNILNPEETSFFDEDGKAIKSILLIST
                     KLQTSTPFDQESNRYETSTLRQAEIALASQTLSPKEAEVIFNTTQPGGDVVTVTSYSF
                     IESQLFGDKFFAPTAKIMNSNSCGLEDRHYRQEGNYYWLSAAAKDDPKLAGYISKCGV
                     FAQLDITVEHLSARATANLDPHAVLFFAAAQGGKVSGVVGSKAMQDVEHQELLSDYRL
                     TLKDASQTLVVSDQVKENNKISFNYNAQGDGDLLSAVIIDKTQNEIVSYGQLADISVT
                     KMGVALIELPKKYDHDNYQIKVFSEQYNGDYKTDYASELVDLKIE"
     gene            complement(1507..2103)
                     /locus_tag="PLK68_06710"
     CDS             complement(1507..2103)
                     /locus_tag="PLK68_06710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM90136.1"
                     /translation="MTEKEKMLAGELYDCGDAELLALWHRAKDLARDYNLTNSSDTKR
                     KSELLNELLGKVGNQLWITPPFHVDYGCNIYFGNNCEVNMNCTFLDDNKIIIGDNVLI
                     APNVQIYTAYHPTHYLDRFTISENETFNFCKTQTAPVIIGKNVWIGGGTIILPGVTIG
                     DNTVIGAGSVVTKDIPADTIAYGNPCKVHKANERSKSI"
     gene            complement(2104..2730)
                     /locus_tag="PLK68_06715"
     CDS             complement(2104..2730)
                     /locus_tag="PLK68_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM90137.1"
                     /translation="MKKYKAIIYDLDGTVLNTINMNMYPLIKIIKEETGEDWTFEQVL
                     KFLPYPGMKVMEELQVADKEKAYARWVKYVNEYEEGASLYPGFEEIFEAFDGSIIQAV
                     ASAKTTAQYQIDFVAKGLDKYMKTAVLANDTVKHKPDPEPLLECLKRLSLQPEDVIYI
                     GDAHSDYLASKNAGIDFGYAKWGSVSAKGIDKPDHVFEQPLDLLKLLS"
     gene            complement(2717..3712)
                     /locus_tag="PLK68_06720"
     CDS             complement(2717..3712)
                     /locus_tag="PLK68_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM90138.1"
                     /translation="MTKKILKDVKKHHLHLLLQSLFSRDSATMSELSNDTQLSQPSVR
                     NMIRLLQQHDIVKETGNDHSTGGRCPTRFALVEKHFNIICLYIQNPKITYQIHSYKKI
                     LTTDILEFEDEQQLITLIKVLLTKFNCQCIVIAVEGIVRDNEYLTDHNNHFETHTWIT
                     ELEKSINIPILVENDVKAMQLGTCYHHHKNSSIYLHLNKKGIGSSYMYNGQLVHGKYG
                     IAGEIGLIPYHDISLNQAVRTCNKPKDFQNIIAYLLIIVLTSLDPEHVDLSLELDWDL
                     DWHLINETIYTYLHSQFIYDIKVYKQYLDNLFHGLNYLGIEKILKQLVEESNEKI"
BASE COUNT         1291 a          583 c          657 g         1345 t
ORIGIN      
        1 gtctatgttt ataaacggga gtatgacgga aaaaaattat ttgtcttatc taattttaca
       61 gctaaagagg ttacctatga taaaacttta tttgaagctg aagcaaaggt gttactagga
      121 aattataatg atttgataag agggaaatta cgggcttatg aagcggttgt actagttgtt
      181 taaagttagt attagggatg atcttgaatg atcaggaggt aagaaaatgc ctaaaaaata
      241 ttttaagatt ggttgcactg taattatagt aactttaata ggattatttt taaaaaataa
      301 tgctccggct aataattata aaaatcccga tactttgaat atacaagata aacaaagtat
      361 gatgatggca gataaggctg ttgaaaaaat agctagggga acatccggga agtttgaaac
      421 tttatattta ggggctgatg gtcaatttca atggaatatt ttaaatccgg aagaaactag
      481 cttttttgat gaagatggaa aagctattaa atccatttta ttaatttcaa caaagcttca
      541 aacctcaaca ccatttgatc aggaaagtaa tcgctatgaa actagtactt tacgacaagc
      601 agagattgcg ttagcatctc aaactttatc accaaaagaa gcggaagtaa tatttaatac
      661 tactcaacca ggtggtgacg ttgttactgt tactagttat tcatttatag aatcacagtt
      721 atttggtgat aaattttttg ccccaacagc aaaaataatg aattcaaata gttgtggact
      781 tgaagatcgt cattatcgtc aggaggggaa ttattactgg ttgtcagcag ctgctaaaga
      841 tgatcctaaa ttagcgggat atatctctaa atgtggagtt tttgcacaat tagatattac
      901 tgtcgagcat cttagtgcaa gagctactgc taatcttgat cctcatgcag ttttattttt
      961 tgctgcagcc caaggtggta aagtcagtgg agtagtcggg agcaaagcaa tgcaggacgt
     1021 ggaacatcaa gaattactta gtgattatcg tttaacatta aaagatgctt cacaaacctt
     1081 agttgtgagc gatcaggtta aagaaaataa taaaataagt tttaattata atgcccaagg
     1141 cgatggggat ttactcagtg cagttataat tgataaaaca caaaatgaga ttgtatctta
     1201 tggtcagtta gctgatatta gtgttactaa aatgggtgtt gctttaattg agttgccaaa
     1261 aaaatatgat catgataact atcagataaa agtgtttagt gagcaatata acggggacta
     1321 caaaacggat tatgccagtg aattagttga tttgaagatt gaataaaata aaaagagctg
     1381 gatttaactg taccgacctc ctaaccgtta gaatttgata tgtatctaaa attcagggca
     1441 catatcattt ttgatctaac tttctgaggt cacatcaaac gatttgttaa atctagttct
     1501 tttttattaa atacttttgc tgcgttcatt ggctttatga actttacaag gattgccata
     1561 agcaattgta tcagcaggga tgtcttttgt aacaacacta cctgctccaa ttacggtatt
     1621 atcaccaatt gtaactcccg gtagaataat tgttcccccg ccaatccaaa catttttacc
     1681 aataataact ggggctgttt gtgttttaca aaaattgaat gtttcatttt cactgatagt
     1741 aaaacgatca agataatgag taggatgata agcggtatat atttgtacat ttggagcaat
     1801 taaaacatta tcaccaataa taattttatt atcatcgaga aatgtacaat tcatattaac
     1861 ttcgcagtta ttcccaaaat atatattaca gccataatca acatgaaaag gtggggtaat
     1921 ccataattgg tttccaactt tacctaataa ttcattaaga agctcacttt ttcttttggt
     1981 atcgctagaa ttggttagat tataatctcg ggctagatct ttggctcgat gccatagagc
     2041 aagtaattcg gcatcgccac aatcatataa ttccccagct aacatttttt ctttttcagt
     2101 catttaagat aataacttta atagatcaag tggctgctca aacacgtgat ctggtttatc
     2161 aataccttta gcggaaacac ttccccattt tgcataacca aagtcgatac cagcgttttt
     2221 agatgcgaga taatcagaat gagcatcacc gatataaata acatcttcag gttgcaaaga
     2281 taatcgcttt aaacattcta ataacggttc gggatcgggt ttatgcttga ctgtatcgtt
     2341 agctaaaaca gcagttttca tatatttatc taaacctttt gcaacaaaat caatttgata
     2401 ctgtgctgtt gtttttgctg aagcaactgc ttgaatgatg cttccgtcaa atgcttcaaa
     2461 aatttcttca aaccctgggt ataaagaagc gccttcttca tattcattga catatttaac
     2521 ccatctagcg taagcctttt ctttatcagc tacttgtaac tcttccatta ctttcatacc
     2581 tggatagggt aaaaatttta agacttgttc aaatgtccaa tcttcaccag tttcttcttt
     2641 aataatctta atcaacggat acatattcat atttattgta tttaatactg ttccgtcaag
     2701 gtcataaata attgctttat attttttcat tactttcctc cactaattgt tttaatattt
     2761 tttcaattcc taaataattt aacccatgga ataagttatc taaatattgt ttataaactt
     2821 taatatcata aataaattgt gagtgtagat aagtataaat agtttcatta attaaatgcc
     2881 aatccaagtc ccaatcaagt tcaagagaaa ggtcaacatg ctcagggtct aaagacgtta
     2941 aaacaattat taatagataa gcaataatgt tttgaaagtc ttttggcttg ttacatgtcc
     3001 taactgcttg atttaaggaa atatcgtgat aaggaataag tcctatttca ccagcaattc
     3061 catatttgcc atgaactaat tgtccattat acatatatga actaccaatt ccctttttgt
     3121 ttaggtgcaa ataaattgaa ctatttttat gatgatgata acaagtacct aattgcatcg
     3181 ctttgacatc gttttcaact agaataggaa tattgatact cttttctaac tctgtgatcc
     3241 aagtgtgggt ttcaaagtgg ttattgtggt cggttaaata ttcattatct ctaacgattc
     3301 cttcaactgc aataacgata cattgacaat tgaatttggt gagaagcact ttaattagag
     3361 taattaattg ttgttcatcc tcaaattcca gaatatcggt tgttagtatt tttttgtaag
     3421 aatgaatttg ataagttatt ttagggtttt gaatatagag acagattata ttaaaatgct
     3481 tttcaactaa tgcaaagcgg gttgggcaac gtccaccggt tgaatgatcg ttacctgttt
     3541 ctttaacaat atcgtgttgt tgcaaaaggc gaatcatatt tcttacacta ggctgtgata
     3601 attgagtatc attgctcaat tcgctcattg tggcactgtc tctactaaat aatgattgta
     3661 ataatagatg taagtgatgt ttttttacat ctttaagaat ttttttggtc atgtgtgcct
     3721 cctgctttta ttatacttta ataaaagtta tttacaagct aataataaaa aataaatata
     3781 tttttaaaaa tggaaagatt ttatctgctg gtttatattt tatggaaatt tatgctagaa
     3841 ttaaagataa taagttaaac tattaataat atgggg
//
LOCUS       DAOMTA010000204         3871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_786_length_3871_cov_2.109015, whole genome shotgun sequence.
ACCESSION   DAOMTA010000204 DAOMTA010000000
VERSION     DAOMTA010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_3871_cov_2.109015"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLK68_06725"
     CDS             <1..864
                     /locus_tag="PLK68_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRM90139.1"
                     /translation="IIFFTCFHLEKNVIIDEIITMFLSIFTGKYWYFTAYVFLYLLIP
                     FINRFIFSLKKNELRNLIFILLFVFAFVPWISEKFDNKVFGVDGGYSIIWLIVLYIIG
                     AAIRIYGPDIIMLKQKSSKKYFVWAVFCGLIIGMSKIFIAYITINIFGHDFLSNILIS
                     YISPICVLESIYIFAFFTTFTMTTSKINNNLILKISRITFDVYIIHLTSLFKKYLWPS
                     IAIYISQNNFLIVLSYIIVPCIVLFLCYVVKNITDCIIKILNIENVNNYVCLKVKLIL
                     NRLVIKDMEVK"
     gene            864..1691
                     /locus_tag="PLK68_06730"
     CDS             864..1691
                     /locus_tag="PLK68_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90140.1"
                     /translation="MQLNTTSYQNILDSLSNQLNLKEKEILNTIDSGYYIFQQNHQIL
                     YIDDLYECYFNIVKRNFKGNIDKVPFYSISRRLKDTDNDGLSLLELLTEENSFSNYLK
                     EYGLTFKFDKEIEMYVNDNKVNIPDDKKYKPYLKNRFSYDYSFKGYAFDDQLMNNEIL
                     ERVKYGPEFFGHLFNYVDNDDEIIDNYLEQSKLYKFEYLVPIEDIYFENYEELTNEEK
                     QYHILAMMMLRLYFYKYDKDFVETDEMNPLMVVANYKSLSSKYLVNKTELDDEALGY"
     gene            1752..2027
                     /locus_tag="PLK68_06735"
     CDS             1752..2027
                     /locus_tag="PLK68_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM90141.1"
                     /translation="MEVFTFLAKTIIGLNIHLCCLIERLIKKEEIVSHINIVDFEKNN
                     QEFINGVRISFKAIANQYNITIPISEIAYIYDYVLNNYKTDMSIDEI"
     gene            2051..2788
                     /locus_tag="PLK68_06740"
     CDS             2051..2788
                     /locus_tag="PLK68_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRM90142.1"
                     /translation="MALVQVNFISQSLKRTVSMNVILPVDKFLFKGNCQPVKEYKTLY
                     LLHGLLGNYTDWVTRTRIQEWAEAKNLVVVMPSGDNSFYVDQEVKNNDFGIFIGTELI
                     EVTRRMFHLSNRRDDTFIAGLSMGGFGALRNGLKYYQNFGYIAALSSALNIFELPVHD
                     ESRCVMGEDSCFGDIDEAYLSDKNPKVCLENLIQAKKEDNTIVFPKIYMACGCDDELI
                     GVNRKFKGYLENAGFDLVYKEDVGSHN"
     gene            complement(3082..3414)
                     /locus_tag="PLK68_06745"
     CDS             complement(3082..3414)
                     /locus_tag="PLK68_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRM90143.1"
                     /translation="MSITVNIYYTGINGNARKFAEEMVTSGIVDAIKAEKGNERYEYF
                     FSMNDPETILLIDSWKDQKSIDAHHATIMMEQITELRNKYDLHMKVERYTSNEENITS
                     ADKKFIRL"
     gene            3604..>3871
                     /locus_tag="PLK68_06750"
     CDS             3604..>3871
                     /locus_tag="PLK68_06750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534690.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HRM90144.1"
                     /translation="MNTKQLERMQYGKGFIAALDQSGGSTPKALKSYDIDESAYSNDK
                     EMFDLIHQMRSRIITSKAFTSKHILGAILFEKTMDSQIEGVDTAE"
BASE COUNT         1431 a          387 c          602 g         1451 t
ORIGIN      
        1 ataatctttt ttacatgttt tcatcttgaa aaaaatgtta ttattgatga aataattaca
       61 atgtttttat ctatttttac tggaaaatat tggtatttta ctgcatatgt atttttgtac
      121 ttattgattc cattcataaa tagatttata tttagcctaa aaaaaaatga attaagaaat
      181 ttaatattta ttttgctatt tgtatttgct tttgtcccat ggatttctga aaaatttgat
      241 aataaagtat ttggcgttga tggaggatac agcataattt ggctcattgt attatacatt
      301 attggtgcag caataagaat atatggtcca gatataatta tgcttaaaca aaaatcatca
      361 aaaaaatatt ttgtatgggc cgtattttgt ggattgatta ttggaatgtc aaaaatattc
      421 atagcatata taactataaa tatttttggt catgattttc tatcaaatat acttataagt
      481 tatatttcgc ctatttgtgt tttggagtct atttatatat ttgcgttttt tacaacattt
      541 acaatgacaa ccagcaagat taataataat cttatattaa aaatctcgcg aattactttt
      601 gatgtatata taattcattt aacctcatta tttaagaaat atttatggcc tagtatagct
      661 atatatatat cacaaaataa ttttttgata gttttatcat acataattgt tccgtgtata
      721 gttttattct tatgttatgt tgtaaaaaat ataacagatt gtattattaa aatattaaat
      781 attgagaatg taaataacta tgtttgttta aaagtaaaat taatattgaa tcgtttggta
      841 ataaaagata tggaggtgaa ataatgcaat taaatactac atcatatcaa aatatcttag
      901 attctttatc gaatcaatta aacttaaaag aaaaagaaat tttaaatact attgattctg
      961 gttattatat atttcaacaa aatcatcaaa tactatatat tgatgattta tatgaatgct
     1021 atttcaatat tgtaaaaaga aattttaaag gaaatattga taaagtgcct ttctatagta
     1081 tatctagaag attaaaagat actgataatg atggattaag tctgttagaa ttattaacag
     1141 aagaaaatag tttttcaaac tatttaaagg aatatggatt aacatttaaa tttgataaag
     1201 aaatagaaat gtatgttaat gataacaaag ttaatattcc cgatgataag aaatataaac
     1261 cctatctaaa aaatagattc tcatatgatt attcatttaa aggttatgct tttgatgatc
     1321 aattaatgaa taatgagata ttagaaagag ttaagtatgg tccagagttc tttggacatc
     1381 tttttaatta cgtagataac gatgatgaaa tcattgataa ttatctagaa caaagtaaat
     1441 tgtacaaatt tgagtactta gtacctattg aagatattta ctttgaaaat tatgaggaac
     1501 taactaatga agaaaaacaa tatcacatct tagcgatgat gatgttaaga ttgtatttct
     1561 ataaatatga taaagatttt gtagaaactg atgaaatgaa tccacttatg gtagtggcta
     1621 attataagtc tctttctagc aaatatcttg taaataaaac tgagttagat gatgaggcac
     1681 taggctatta gtctagtgct tttttattct atataatatc tgaaaattat gaaaagatcc
     1741 attttatata attggaagtt tttacttttt tagcaaaaac tattattggt ttgaatattc
     1801 atctttgttg tttgattgaa cgtctaataa aaaaagaaga gatagtttca catattaata
     1861 ttgttgattt tgaaaaaaat aatcaagaat ttatcaatgg agttagaatt agctttaaag
     1921 cgattgctaa tcagtataat ataacaatac caattagtga aattgcttat atttatgatt
     1981 acgttttaaa taattataaa acagatatgt caattgatga gatttaaaaa gagtaaaggg
     2041 gtgttttatt atggcattag tacaagtaaa ttttatttct caaagtttaa aaagaacagt
     2101 ttcaatgaat gtaatattac cggttgataa atttcttttt aaaggaaatt gtcaaccagt
     2161 taaagaatat aaaactttat atttattaca tggtttatta ggaaattata ctgactgggt
     2221 aacacgaaca agaatacaag aatgggcaga agcaaagaac ttagttgtag tcatgccatc
     2281 tggtgataat tctttttatg tggatcaaga agtaaagaac aatgacttcg gtatatttat
     2341 tggaactgaa cttattgaag ttactagaag gatgtttcat ttatctaatc gtcgggatga
     2401 tacttttatt gccggacttt caatgggtgg atttggtgct ctaagaaatg ggttgaaata
     2461 ttatcaaaat tttggatata ttgcagcttt atcaagtgct ctaaatattt tcgaattacc
     2521 agttcacgat gaatcaagat gtgtaatggg tgaagatagt tgttttggtg atattgatga
     2581 agcatatctg agtgataaga atccaaaagt ttgtttggaa aatttaattc aggcaaaaaa
     2641 agaagacaat actattgttt ttcctaagat atatatggct tgtggatgtg atgatgaact
     2701 aattggagta aatcgaaaat ttaaaggcta tttagaaaat gctggttttg atttagttta
     2761 taaagaggat gtaggaagtc ataattgaga tttttggaat aaatatattc aagatgtatt
     2821 agaatggtta cctttggatc cttatgaaga aggaattaac agcggtaatg ttaaataggc
     2881 aatgtttttt tattagaatt atatatttat aattaaaagc tatatttact tggtaataaa
     2941 gaacgataat tgtgacagga taaattataa ctaaaatggt aaataaggaa taaatatttc
     3001 aattaccaga aatatgtttt gatatgaata gtataaaacc cattataata ttgactttat
     3061 aatgggtttt attaatttag attacaatcg aataaatttt ttgtcagcag aagttatgtt
     3121 ttcttcatta gaagtatagc gttctacttt catatgaaga tcatatttat ttcttaattc
     3181 tgtaatttgc tccatcatta tagtagcatg atgagcatca attgattttt gatctttcca
     3241 actatcaatt aaaagtatgg tttctggatc attcatggaa aagaaatact catatctttc
     3301 atttcctttt tctgctttaa tagcatcaac aataccgctg gtcaccattt cttctgcaaa
     3361 ttttctagca tttccgttta ttccagtgta ataaatgttt acagtgatag acatattaat
     3421 tatctcctta tgtataaaga ttatcttatt taattatagc atattttaaa ataatctatc
     3481 atatttttaa aagcgattct tatttgtaaa aatagttatt cttatctaac gaaatagaac
     3541 gtattattcg aatacttgat ggacaagggg tataataagg gcaggaaaag gaggtgctct
     3601 taaatgaaca caaaacaatt agagcgtatg caatatggaa aaggttttat cgcagcgtta
     3661 gatcaaagtg gtggaagtac accaaaggca ttaaaatcat atgatattga tgaatcagcg
     3721 tatagtaatg ataaagaaat gtttgattta attcatcaaa tgcgtagtcg tattattact
     3781 agtaaggctt ttacctctaa gcatatatta ggtgctattt tgtttgaaaa gacaatggat
     3841 tctcaaattg agggtgtaga tactgctgaa t
//
LOCUS       DAOMTA010000205         3833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_801_length_3833_cov_2.204076, whole genome shotgun sequence.
ACCESSION   DAOMTA010000205 DAOMTA010000000
VERSION     DAOMTA010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_3833_cov_2.204076"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLK68_06755"
     CDS             <1..481
                     /locus_tag="PLK68_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HRM90145.1"
                     /translation="KGYGKSLMDYCLADAKAKGKSGVCLLGSKKQKHWLTDQSFAKSN
                     GFEVVDTTDDGYELLALSFDGTFPRFTQKAKKQKIENKELTIYYDMQCPFVYQSLEIV
                     KQYCEINNVPILMIQVDTLQKAKDLPCVFNNWAVFYHGEFETVNLLDKNTLTRLLKK"
     gene            678..1355
                     /locus_tag="PLK68_06760"
     CDS             678..1355
                     /locus_tag="PLK68_06760"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HRM90146.1"
                     /translation="MKRIQNSLIVSCQALEDEPLHSSLIMSKMALAAKMGGAKGIRAN
                     SVQDIHEIKKEVDLPIIGIIKKDYEDTDIYITTTMKEVDALVAEGVDIIAMDATMQLR
                     PHNQTITEFFKEVKAKYPHQLFMADCSTVEEAIHADNIGFDFIGTTLVGYTPQSQNSR
                     IDANDFKIIREIVKNVKHPVIGEGNIDTPSKVKRALELGCYSVVVGSMITRPQLITKK
                     FVDEISR"
     gene            1371..1838
                     /locus_tag="PLK68_06765"
     CDS             1371..1838
                     /locus_tag="PLK68_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90147.1"
                     /translation="MMDKLSNVLLPIAEKLSKNRYLTAIRDGFVSIMPLVITASLFTL
                     INSVFVGEGHYFDQWFGAPCNDFAKIGSVISSASMSIMAILLVFTTAKALANEYKMDT
                     SVAGATAVVCFLCLTPFVSDAKIGEYVTTYYLGAAGMFTAFISALISVQSTGY"
     gene            1840..2619
                     /locus_tag="PLK68_06770"
     CDS             1840..2619
                     /locus_tag="PLK68_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90148.1"
                     /translation="MPESVPTGIARSFNAIIPVALTVIIFAILRIITDAIGAPLNDLI
                     FNWIQTPFTAIVSSPVGLIVIYVLYMLLWGFGIHSAYIFNPILEPIYLASLAINEAAI
                     TSGTAATEIITKPFIDSVAFMGGAGNMLALVIAIFIVSKRDDYKVIGKLGFVPALFNI
                     SEPLMFGLPVVMNPILIIPMILTTLAGLGIGALATQIGIMAHTYVLIPWTTPPVISAF
                     LATGGDIMSGVIGLLILVVSVLIYIPFVKVMNKEGIESIEE"
     gene            2609..3376
                     /locus_tag="PLK68_06775"
     CDS             2609..3376
                     /locus_tag="PLK68_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM90149.1"
                     /translation="MKNKVIVFDLDGTLLGANNEIIGGTETLNCLDSLQQMGWTLAIC
                     TGRLDHDILKIEEQYKLKIEHRISQNGAVCMKGQQLIATLIDKQEAINIYKEIQKEDI
                     RIELNTVSNRYWKSERDPAFPSELYDSHIICKDFDKLILYQPAVLFLVIGTEDKLRSI
                     ETYINTTYQKTKAVMTSSTSLEIMHKDASKGNAVKMLYPKSEVYAIGDSPNDFDMFPI
                     AKKGYLVANKPCQFPCTQKESILEALKDIIKINKEDR"
     gene            3378..>3833
                     /locus_tag="PLK68_06780"
     CDS             3378..>3833
                     /locus_tag="PLK68_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HRM90150.1"
                     /translation="MKTLFIPLDERPCNYNFPLMIAQSNQQIELVEPDKSFLGHKKKP
                     AQVEYFKQFIDENIEGCDNCIISIDMIVYGGLIPSRLHYLSETDALQRLELIKYLKQL
                     KPQIKIYAYHCIMRAPSYNSSEEEPDYYENYGFALFRRKYLLDLKKRHGL"
BASE COUNT         1331 a          512 c          732 g         1258 t
ORIGIN      
        1 caaagggtat ggtaagtcac tgatggatta ttgtttggcg gatgctaaag caaaaggaaa
       61 atcaggtgtc tgtttactag gttcaaaaaa acaaaaacat tggcttacag atcagtcatt
      121 tgctaaaagt aatggctttg aggtcgttga tactactgat gatggatatg aactattagc
      181 gttgtctttt gatggaactt tccctcgatt tactcaaaag gctaaaaagc aaaagattga
      241 aaataaagaa ctaactattt attatgatat gcaatgccct tttgtttatc aaagtcttga
      301 aatagtcaaa cagtattgtg agattaataa tgtacctatt ctgatgattc aagttgatac
      361 tttacaaaaa gctaaagatt taccttgtgt ttttaataat tgggcagtgt tttatcatgg
      421 ggaatttgaa actgtgaatc tgctagataa aaatactctt acaagattat taaaaaaata
      481 gagattataa atatataaag attggaatta aatggctgaa taagccattt ttttaatttt
      541 tattttaaaa gtactttcaa aaacattaaa aataagttgg aataaaaaat aaaaatgaaa
      601 gtaattacta aagcaagtga atatattgct taataaaata aaagccatta aagttataaa
      661 caaggaggag agatttaatg aagaggattc aaaatagttt gatcgtttca tgtcaggcat
      721 tagaagatga accgctgcat tcatcattga tcatgtcaaa aatggcactt gctgcaaaga
      781 tggggggagc aaaaggaata agagcaaata gtgttcaaga tatccatgaa attaaaaagg
      841 aagtggattt gccaattatt ggtatcatca agaaagatta tgaggataca gatatttata
      901 ttactacaac gatgaaagag gtggatgctc tagtggcaga aggggtagat attattgcga
      961 tggatgcgac aatgcagtta agacctcata atcaaacaat tactgaattt tttaaggagg
     1021 taaaagcaaa atatcctcat caattgttta tggctgattg ctccactgtg gaagaggcaa
     1081 tccatgctga taatattgga tttgatttta ttggaacaac tttagtcgga tacacaccac
     1141 aaagccaaaa tagtcgaatt gatgctaatg attttaaaat tattagagag atagtgaaga
     1201 atgttaagca tcctgtgatt ggtgagggaa atatagatac tccttctaaa gtaaaaaggg
     1261 cattagaatt aggttgttat agtgtggttg taggatcaat gattacaaga ccacagttaa
     1321 ttacaaagaa atttgtagat gagatttcaa gataagggag gaaagagatt atgatggata
     1381 aattgtctaa tgtgttattg ccgattgctg agaaactttc taaaaatcgt tatttgacag
     1441 caattaggga tggttttgtt tcaatcatgc cgctagtaat tactgcgtct ttatttacat
     1501 tgatcaatag tgtatttgta ggtgaggggc attactttga tcaatggttt ggagcaccat
     1561 gtaatgattt tgctaaaatc ggtagtgtaa ttagttcagc ctcaatgtca attatggcaa
     1621 ttttgctagt ttttactact gctaaagctt tagctaatga atataaaatg gatacttcgg
     1681 ttgctggagc aacagcagtt gtttgctttt tatgtctaac accatttgta agtgatgcaa
     1741 aaattggtga atatgtgact acttattatt taggggcagc tgggatgttt acagcattta
     1801 tttctgcctt gatttcagtt caaagcactg gttattaaaa tgccggaaag tgtacctact
     1861 ggaattgcta gatcatttaa tgcaattatt cctgtagctt taacggttat tatttttgca
     1921 atcctgagaa ttataaccga tgcaataggg gcaccgctta atgatttgat ttttaactgg
     1981 attcaaacac cttttactgc aattgtttca tcaccggtcg gattgattgt tatttatgtt
     2041 ttgtatatgc ttttatgggg ttttggaatt cattcggcat atatttttaa tccaatctta
     2101 gaaccaatct atttggcaag tctagcaatc aatgaagctg ctattacaag tggaacagca
     2161 gctaccgaaa ttattactaa accatttatt gattccgttg catttatggg tggcgctggt
     2221 aatatgttag cgcttgtaat tgctattttt atagtttcta aaagagatga ttataaggtg
     2281 attggaaaat taggttttgt accagcatta tttaatattt cagaaccgct gatgtttgga
     2341 ttacctgttg ttatgaatcc tatcttgatc attccaatga ttttaacaac tcttgctggt
     2401 ttaggaattg gagcgttagc tacacaaatt ggtattatgg ctcatactta cgtattgatc
     2461 ccgtggacga ccccaccagt aatcagtgcg tttctagcta ctggaggaga tattatgtca
     2521 ggggtcattg gtctacttat tttggtagta tctgttctta tctatatccc atttgtaaaa
     2581 gttatgaata aagaaggaat tgagagtatt gaagaataaa gttattgttt ttgatctaga
     2641 cggtacactt ttaggtgcca ataatgaaat cattggagga accgagacat tgaattgtct
     2701 tgattctctc caacaaatgg gatggacatt agcaatatgt acaggtcggt tagatcatga
     2761 tattttaaaa atagaagaac aatacaaact taaaatagag catcgtattt ctcaaaacgg
     2821 tgcggtttgt atgaaaggac aacaattgat agctaccttg attgataaac aagaagctat
     2881 aaatatctat aaggagattc aaaaagaaga tattcgaatc gagttaaata cagtatctaa
     2941 tcgttattgg aaaagtgaaa gagatccagc tttccctagt gaattatatg attcccatat
     3001 catttgtaaa gattttgata aacttatact gtatcagcca gcagttttgt ttcttgttat
     3061 tggtactgaa gataaacttc gctcaataga aacatatata aatacaacat accaaaagac
     3121 aaaagcagtg atgacttctt caacatcttt ggaaatcatg cataaagatg cctcaaaagg
     3181 aaatgccgta aaaatgctat atcctaaaag tgaagtatat gcaataggtg attccccaaa
     3241 tgactttgat atgtttccaa ttgctaaaaa gggttactta gttgcaaata agccttgtca
     3301 atttccctgt actcaaaaag aaagtatttt agaagcgtta aaagatataa taaaaataaa
     3361 taaagaggac agataaaatg aaaacattat ttattccttt agatgaacga ccatgcaatt
     3421 ataattttcc tttaatgatt gcccagtcaa atcaacaaat cgaattagtt gaaccggata
     3481 aaagtttttt aggtcataaa aagaagccgg ctcaggtgga atattttaaa caatttatcg
     3541 atgaaaatat tgaaggatgt gataattgta ttatctcgat agatatgatc gtatatggtg
     3601 gattgattcc atcacgatta cactatttga gtgaaacaga tgcgcttcaa cgtttagaac
     3661 taattaaata tctaaaacag ttgaaacctc aaataaaaat atatgcttat cattgtatta
     3721 tgcgtgcacc aagttataat tcgagtgaag aagaacctga ttattatgaa aattacggct
     3781 ttgctctttt tcgaagaaaa tatcttcttg atctaaaaaa aagacatggg tta
//
LOCUS       DAOMTA010000206         3831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_803_length_3831_cov_2.914460, whole genome shotgun sequence.
ACCESSION   DAOMTA010000206 DAOMTA010000000
VERSION     DAOMTA010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_3831_cov_2.914460"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLK68_06785"
     CDS             complement(<1..772)
                     /locus_tag="PLK68_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM90151.1"
                     /translation="MSFFTFLEEKITEILFQLFFLAVVAFLLIFYGVDTLFVVSLVIL
                     FISIQGLFQWCLYRKKRNASQHIIDLVDGLEETYYIADVLPKPKEFQNKAYCYALKKA
                     CKSMNDEIGKITEEKQDYQEYVESFVHEIKIPIGALSLTFDNTKNYTLKKETDKIFQL
                     VEQMLYYARSENTEKDYFVKQLQLDEVIHNVILKFRHSLMERKAIINIHDIENVIYTD
                     EKWLTFILSQIVQNAIKYFDKQENKLTIYSQDNGTNILL"
     gene            complement(769..1428)
                     /locus_tag="PLK68_06790"
     CDS             complement(769..1428)
                     /locus_tag="PLK68_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HRM90152.1"
                     /translation="MRIAIIEDDEITRLELSKLLNTQGYETVLLTDFGNLTEELKQYS
                     IELVLLDINLPYENGYEVCRKIKQVMPVPIIFVTSRDTNADELKSIQVGGIDFITKPY
                     DTLILLEKIKRALQLSNPNNFRELVKKDCTLDLHLSILKYQEKSIELTRNEFCILYYF
                     FMNEDKVISKEELLEKLWNDKYYLDENVLLVNMTRLKKKMKEIGIVHLLENIRGKGWK
                     L"
     gene            complement(1487..3463)
                     /locus_tag="PLK68_06795"
     CDS             complement(1487..3463)
                     /locus_tag="PLK68_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90153.1"
                     /translation="MLGKLAVRNTKRNLKDYLIYLITITISFSLILAFNLIASSDEVV
                     KLSSGMDSFKNVLTFVNIIIVFVVCFLINYTTRFMFEKRSKEFGTYMLLGIKKKEVAR
                     LLVMENILLGFLALILAIPLGFLFSQFVSLVIVKLLGIPEVIFISLNLISVGLLVIYF
                     LAIYVLVLLNLLRRIRKMTVHDFLYFDKQNEKKMFRNNKKRNIIFMVSAVLGVSSLLL
                     WNSRCSFENFSDESTMTYLMICVIMLIISIYGISATCADMLLSILLKSKKMKYQNDNL
                     FVTRTFASKARTMSFTFGTLSMLILLSLLCLNFSSIYKGLYQTSIELTSPYDVEVFDW
                     TQPFDDYKEYLDVIDEDYTIKETIEYNVYKEPKHQIQDYYNVQFYDYDPVMKLSDYNR
                     LLKLRNMNTVELTEDEYFLVTDSQLLYKVEDNADMQTIQFANNELRLKGIDTKSFWYS
                     MNNTGRFTVIVPDEYVQGLEISEEHLIVDTKEETTAKLEEKIDTELKHLLVVEDEDGT
                     THNEYYRVSVRGTVIEEQNTMTAIMASICLYIAFILISAVGTILAVQSLSDATKYKYR
                     YQTLRRLGVNDKSLFKTIKKQLLILFGIPVVYSVIASFCMLASLNNVYNVLLANEFIY
                     LFYFAGGLAIFFFIYGIYWIATYIGFKRNINEES"
     gene            complement(3453..>3831)
                     /locus_tag="PLK68_06800"
     CDS             complement(3453..>3831)
                     /locus_tag="PLK68_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90154.1"
                     /translation="MMGLKDWATHYPNEMSGGQKQRCACARALINEPKLILADEPTGA
                     LDSKSSRLLLETMSDINKKMQATILMVTHDPFSASFCKRILFLKDGKLFNEIFRGEKS
                     RKDFFNEILDMLTLLGGEVGNVR"
BASE COUNT         1248 a          714 c          532 g         1337 t
ORIGIN      
        1 caagcaatat atttgtacca ttgtcttgac tgtatatcgt cagtttattt tcttgcttat
       61 caaaatactt aatcgcattt tgcacgattt gagatagaat gaatgtcagc catttttcat
      121 cagtataaat gacattttct atgtcatgga tattgataat tgcttttctt tccataagcg
      181 aatgacggaa tttcagaatg acattatgta tcacttcgtc taactgcaac tgttttacaa
      241 aatagtcctt ttccgtattt tcgcttcttg catagtagag catttgttcc actaactgaa
      301 atattttgtc tgtttctttt tttaatgtgt aattttttgt attgtcaaac gtcaaagaca
      361 atgctcctat cggtatttta atttcatgga cgaaactttc cacatattct tgatagtcct
      421 gcttttcctc tgtgattttg ccgatttcat cattcataga tttgcaggct ttttttagtg
      481 cgtagcaata ggctttattt tgaaattctt ttggctttgg taaaacgtct gcaatataat
      541 aggtttcttc cagtccgtct actaaatcaa taatatgttg tgaagcgtta cgtttcttcc
      601 gatacaaaca ccattgaaaa agcccttgta tgctgatgaa cagaattacc aatgatacaa
      661 caaacagcgt atctacaccg tagaaaatca agagaaatgc tactacggca agaaagaaaa
      721 gctgaaacaa tatctctgtt attttttctt ctaagaatgt aaaaaaactc acagtttcca
      781 accctttcct cgtatatttt ccaataaatg aacaatcccg atttctttca ttttcttttt
      841 tagacgagtc atattaacca gtaatacatt ttcgtctaaa taatatttgt cgttccacag
      901 cttttccaat agttcttctt tgctaataac tttatcttcg ttcataaaga agtagtataa
      961 aatacaaaat tcatttcttg tcagttcaat gcttttctct tgatacttca gaatggataa
     1021 atgtaaatca agggtacaat cttttttgac aagctcacgg aaattattcg ggtttgacaa
     1081 ctgcaatgca cgcttgatct tctcaagaag aataagcgtg tcatacggct ttgtgatgaa
     1141 gtcaatccca cccacttgaa tacttttcaa ttcgtcagca tttgtatctc tgcttgttac
     1201 aaagataata ggtactggca tgacttgttt tattttcctg catacttcgt agccattctc
     1261 atacggcagg ttaatatcaa gcagaaccag ttctatggaa tattgcttta attcctctgt
     1321 cagatttcca aagtcagtca ataaaactgt ttcatatcct tgtgtgttca atagtttaga
     1381 aagctccaaa cgagtgattt catcatcttc gataatcgct attctcattt caacaccccc
     1441 ttgatttacc attataataa ttccacagca ggagagctag aaaaatctaa ctctcctcat
     1501 tgatatttcg tttgaaaccg atatacgtcg caatccaata aataccatag ataaagaaga
     1561 aaatcgccaa accaccagca aaatagaaca ggtagataaa ttcatttgcc aaaagaacgt
     1621 tataaacatt gttaagtgaa gctaacatac agaaacttgc aataaccgaa taaacaactg
     1681 gaattccaaa taatatcaat aattgttttt tgattgtctt aaacaatgac ttgtcattta
     1741 ctcctaatct tcgcaaagtc tgatagcgat atttgtattt agtagcatca cttaaagatt
     1801 gcacagcaag aattgttccc acagcagaga ttaaaataaa agcaatatat aaacagatac
     1861 ttgccatgat agcagtcata gtattttgtt cttctataac agtacctctt actgatactc
     1921 tataatattc attatgagtt gtcccgtctt catcttctac caccaataaa tgctttaatt
     1981 ctgtatctat cttttcttct aattttgcag tcgtttcttc ttttgtatca acaattaaat
     2041 gttcttctga tatttctaat ccctgcacat attcatcagg aacaatgact gtaaatcttc
     2101 ctgtattatt catactgtac caaaaagatt ttgtatcaat cccttttaag cgaagttcat
     2161 tgttagcaaa ttgaatggtc tgcatatcag cattatcttc aactttgtat aatagttgac
     2221 tatcagttac aaggaaatac tcatcttctg ttaattcaac agtattcata tttcgtaatt
     2281 tcaataatct gttataatca cttaacttca ttactgggtc ataatcataa aactgcacat
     2341 tgtaataatc ttgtatttga tgtttaggtt ctttataaac attatattca atggtttcct
     2401 tgattgtata atcttcatct ataacatcaa gatattcttt ataatcatca aaaggttgtg
     2461 tccaatcaaa aacttctaca tcataaggag aggttaattc aatgctcgtc tgatataaac
     2521 ctttatagat actggaaaaa ttcagacata gcaaggaaag taatatcagc atagaaagcg
     2581 ttccgaatgt aaaactcatt gttcttgcct tcgacgcaaa agttcttgtt acaaataggt
     2641 tgtcattttg atatttcatt tttttacttt ttagtagaat tgataataac atatccgcac
     2701 aagttgcaga aataccataa atactgatta ttaacataat cacgcaaatc attaaataag
     2761 tcatagttga ctcatcactg aaattttcaa aactgcaacg tgagttccaa agcagaagtg
     2821 aggaaacccc cagtacagca ctaaccataa agataatgtt tcttttttta ttattacgga
     2881 acattttctt ttcattctgt ttatcaaagt aaagaaaatc atgtaccgtc atttttctta
     2941 ttcttcgtaa aaggttaagt aaaaccaata catatattgc caaaaagtaa attacaagta
     3001 accctacgga aataaggttt aatgaaatga atataacctc tggaatacct aataatttta
     3061 caatgactaa tgacacaaac tggctaaaca gaaaaccaag tggaatagct agtataagtg
     3121 ctaaaaagcc taataatata ttctccatga caagtagacg tgcaacttct tttttcttta
     3181 tcccaagaag catatatgtt ccaaattctt tacttcgttt ctcaaacatg aaccttgtcg
     3241 tatagttaat caagaaacac acaacaaata caataatgat atttacaaaa gttagaacat
     3301 tcttgaatga gtccatacca gatgatagct ttacaacttc atcagaactt gctataagat
     3361 taaatgccag tatgagtgaa aatgaaattg taatggttat cagataaatc aaataatcct
     3421 ttaaatttct ttttgtattt ctaaccgcca atttacctaa catttcccac ttcacctccg
     3481 agcaaggtta acatatccag tatttcattg aagaaatctt ttctgctttt ttcccctctg
     3541 aaaatttcat tgaatagctt accgtctttc aagaacaaaa tacgtttaca aaaagacgca
     3601 ctaaacgggt catgtgttac cattaaaata gtagcctgca ttttcttatt gatgtcagac
     3661 attgtttcca gtaacaatct ggaagatttt gagtccaaag ctcccgtcgg ttcgtcggct
     3721 aaaatcagct ttggttcatt gattaaagct cttgcacaag cacaacgctg tttttgtccg
     3781 cccgacattt cgtttggata gtgggtagcc cagtctttca gtcccatcat g
//
LOCUS       DAOMTA010000207         3796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_822_length_3796_cov_2.789361, whole genome shotgun sequence.
ACCESSION   DAOMTA010000207 DAOMTA010000000
VERSION     DAOMTA010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_822_length_3796_cov_2.789361"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLK68_06805"
     CDS             <1..653
                     /locus_tag="PLK68_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90155.1"
                     /translation="LGAASSAQGFMGLLAAAGFIMHPKQSLQMRLNPASLYGGSRQGG
                     RFSRNGNSEDLGENGLNEGENIDTGFNQNGSMGEGADTGFNQNDSSNEQFDSYGNNMS
                     SEEELGADGNLTGGSGKSENSYSTSSSSPTELQLDSAKYYGIEGAEKMSQQQLKQEFN
                     KRGYGSSFPEQPGYNIDAGIGNSKVNIDNASSNNSVKPASQNSLLNDHSLNKDRRF"
     gene            656..997
                     /locus_tag="PLK68_06810"
     CDS             656..997
                     /locus_tag="PLK68_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90156.1"
                     /translation="MKFIVPQNFKNGRLIMKRYRLFDLVMLISAVGFSLVVFIAVVQS
                     GIGRNGIIFFFAFFSILIFITFILTMGAGINHNILELILTIKDYYKTEKKYEWAGRIY
                     ADEENFKKEQR"
     gene            960..1643
                     /locus_tag="PLK68_06815"
     CDS             960..1643
                     /locus_tag="PLK68_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90157.1"
                     /translation="MQMRKTSKKNKDKKKKVKLTKSDKKIIKERRKVRYTTLDWMSLE
                     EINPDHCVIVEFGNRFILKGIRIMPRNIYLLDDKTLGSIIQGFNVFLNKEKRRLYWKF
                     VKSEPNMIKQNRHLIDLYEDEDDERIKRFIQYELEKNEWFMSSFNEMSFYILILEPEK
                     YIDKHFQDLCNYFKSTRMQFEIAKKKDFQNMIYADFDNRDISDYYIPKLYSEEFNVTK
                     GEEGTDEEF"
     gene            1630..3462
                     /locus_tag="PLK68_06820"
     CDS             1630..3462
                     /locus_tag="PLK68_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM90158.1"
                     /translation="MKSFNDMKNLMFKSRAVPRGFHEYANYYVIGDKFHRAMTFTITK
                     DFDEGFLGMFVAGQNYRVEMVTEPSKAKISNYMKTVVKDMEQEYSKTTDRIQRAELRE
                     QMDGYNAYIRELVRKRDATLNCIITVFIEANTLEELDERTYDLDKLFSTYNVEVRAER
                     FLQQRILKCSSPFFESDGFRKEASQNIGQLLSTQTVSGLYPFTFDTLDDEKGMLLGVE
                     SSQKGMVCFDQFLYLNNPAKAKKYKRLNGNLIIIGESGSGKTTAMDLILMSHICHTRK
                     VIWIDPENKNRTIIKQAGGSYFNLGTKNARINIFDLKPISCDEDEEDEVDKYDTRLAI
                     YNVVEDIKIVFRYLSKDISEGALDILGELVLEAYETVGVNINESFQYLKNTDYPTFSV
                     FSAIVDEHIEKFEKSNIVNDEYTALKEIRIKLKPICTEWARYLDGHTTITEDEMKNPF
                     FAFGTKVFFNLTPGLKNALMHIIFQYAWGLCLDETSESVLAVDEAHMFVLAGETAKMI
                     EQFQRRSRKYHNVTILGTQEPHDFADPRVLAEGKAIFNNASYKLILGLDKDATKDLRK
                     LTALSDNEIKRIARFSQGDAILIAGNKRLPIEIKATPDQLALMR"
     gene            3564..>3796
                     /locus_tag="PLK68_06825"
     CDS             3564..>3796
                     /locus_tag="PLK68_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRM90159.1"
                     /translation="MDFEGGNTRDLFVAKAIDEYNAVNGAVGGDKYRKWYTGSADGAN
                     WCATFVSWVADQCGLIDAKVIPKYQGCDAGVDW"
BASE COUNT         1342 a          463 c          825 g         1166 t
ORIGIN      
        1 agcttggtgc agccagttcg gctcaggggt ttatgggatt gctggcagca gcaggattta
       61 ttatgcatcc taaacagtca cttcaaatga ggcttaatcc cgcatcactt tatgggggta
      121 gccgacaagg aggcagattt tctagaaacg gcaactcgga ggacttaggt gagaacggat
      181 taaatgaagg tgaaaatatt gatactggat ttaatcaaaa tggtagtatg ggtgaaggtg
      241 ctgatactgg atttaatcag aatgatagtt ctaatgagca gtttgatagt tatgggaata
      301 atatgagcag tgaggaagaa ctgggagctg atggtaatct aactggaggt agtggaaaaa
      361 gtgaaaattc atacagtaca tcgagttctt caccaacaga gctacagctg gattctgcaa
      421 aatattatgg catagagggt gctgaaaaaa tgtcacagca gcaattgaaa caggaattta
      481 ataaaagagg atatggcagt tcttttcctg aacagcctgg ttataatatt gatgcaggaa
      541 ttggcaacag taaagttaat attgataatg cttcgagtaa taattcggtt aaaccagctt
      601 cacaaaactc tctattgaat gaccatagtt taaataagga taggaggttt taaaaatgaa
      661 atttatagtt ccgcagaatt ttaaaaatgg acgactgata atgaaacgct atagattgtt
      721 tgacttggtt atgcttatct cagctgttgg tttttcttta gtggtgttta ttgcggtagt
      781 acagtcaggc ataggtcgaa atgggataat attctttttc gccttttttt ctattttaat
      841 ttttataact tttattttaa cgatgggtgc aggaataaat cataacatac tggaactaat
      901 cttaactatt aaggactact ataaaactga gaagaaatat gaatgggcag gtagaatata
      961 tgcagatgag gaaaacttca aaaaagaaca aagataaaaa gaaaaaagta aaattgacaa
     1021 agagtgataa aaaaataatc aaggaaagaa gaaaagtcag atatacaaca cttgactgga
     1081 tgagtttgga agaaattaat cctgatcatt gtgttattgt tgaatttggt aatcgattta
     1141 ttttaaaagg gattagaata atgcctagaa atatttatct gttagatgac aaaacattag
     1201 gcagtataat acaaggcttc aatgtttttt taaataagga gaaaaggcga ctgtactgga
     1261 agtttgtaaa aagtgagcct aacatgatta aacaaaacag acatcttatt gatttgtatg
     1321 aagacgaaga tgatgaacgt ataaaaaggt ttatccagta tgaattagaa aagaatgaat
     1381 ggtttatgtc aagttttaat gaaatgtcat tttatattct tatattagaa ccggaaaaat
     1441 acattgataa acattttcag gatttatgta attatttcaa atctacccgg atgcagtttg
     1501 aaatagcaaa aaagaaagat tttcaaaata tgatctatgc tgattttgat aatagagata
     1561 tcagtgatta ctatatacct aaactatata gtgaagaatt taatgtaact aaaggagagg
     1621 aaggtacaga tgaagagttt taatgatatg aaaaatttaa tgttcaaatc acgtgcagtc
     1681 cctcgtggat ttcatgaata tgctaattat tatgtaattg gtgataaatt tcatcgtgct
     1741 atgacattta caataactaa agattttgat gaagggtttt taggaatgtt tgtagctggt
     1801 cagaattatc gtgtagaaat ggttacagaa ccatcaaagg ctaaaatatc caactatatg
     1861 aaaacggttg taaaagatat ggagcaggag tacagtaaaa ctaccgatcg aattcaaagg
     1921 gctgaattaa gagaacagat ggacggatat aatgcgtata tcagagagct tgtaagaaaa
     1981 agggatgcta cattaaattg tataattaca gtgtttatag aagcgaatac attagaagaa
     2041 ttggatgagc gaacttatga tctggataaa ctgttcagta catataatgt tgaggtaaga
     2101 gctgaacgtt ttttacagca gagaatattg aaatgctcct caccgttctt tgaaagtgac
     2161 ggttttagaa aagaagccag tcaaaatatt gggcagcttc tatcaacgca gacagtaagt
     2221 ggtttgtacc cgtttacttt tgatacattg gatgatgaaa aagggatgct gctaggggtt
     2281 gaatcttctc aaaaagggat ggtatgtttt gatcagtttc tttatttaaa caatccagct
     2341 aaagctaaaa aatataaaag actaaacggc aatcttatta ttataggtga gtctggatca
     2401 ggtaaaacaa ctgcgatgga tctgatttta atgtctcata tatgtcatac tagaaaagta
     2461 atttggattg atcctgaaaa taaaaaccgt actattataa aacaggcggg aggctcttat
     2521 tttaatcttg gtacgaaaaa tgcaagaata aatatttttg atttaaagcc gatttcatgc
     2581 gatgaagatg aagaagacga agttgataaa tacgatacca gattggcaat atacaatgtt
     2641 gttgaggata ttaaaattgt ttttagatat ttatctaaag atatcagtga aggtgctctg
     2701 gatattttag gagagctggt actagaggcc tacgaaactg taggtgtgaa tataaacgaa
     2761 tcttttcaat atttaaaaaa cactgattat ccgactttta gcgttttttc agcaattgta
     2821 gatgagcata tagaaaagtt tgaaaaatcg aacatagtta acgatgaata tacagctctt
     2881 aaagaaatta gaatcaaatt aaaacctata tgtactgagt gggctagata tttggatggg
     2941 catacgacaa taactgagga tgagatgaaa aatccttttt ttgcatttgg aactaaagtg
     3001 ttttttaatt taactccagg attaaaaaat gcgctaatgc atattatatt tcaatatgca
     3061 tgggggttat gcctggatga aacgagcgaa tcggttttag ctgttgatga ggcacatatg
     3121 tttgttttgg ctggtgaaac tgcaaagatg attgaacagt ttcaacgacg ttctaggaag
     3181 tatcataatg taactatttt aggtacacag gagcctcatg acttcgcaga tccgagggtt
     3241 ttggcagaag gtaaagctat ttttaataat gcaagctata agcttatttt aggattagac
     3301 aaagatgcta ccaaagattt aaggaaactt actgcattat cagataatga gattaaaagg
     3361 atagcaagat tttcacaagg ggatgcaata ctgatagcag gaaataaacg cttgccgata
     3421 gaaataaaag ctactccaga tcagcttgca ttgatgagat aaatgaaagc tctgaagtat
     3481 acagtagctt ctttttttac ttttttttag ttcttgtcct aattgtcgca tgtatatctg
     3541 gaggtaaaag taaaacagag gaagtggatt ttgaaggtgg gaatactaga gatttatttg
     3601 ttgcaaaggc aatagatgaa tataatgctg ttaatggtgc cgttggcggt gacaagtata
     3661 gaaaatggta tactggtagt gctgatggag ctaactggtg tgcgaccttt gtttcttggg
     3721 tagcggatca gtgtgggctg attgatgcta aagttattcc taaatatcag gggtgtgatg
     3781 ccggtgttga ttggtt
//
LOCUS       DAOMTA010000208         3794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_823_length_3794_cov_2.785504, whole genome shotgun sequence.
ACCESSION   DAOMTA010000208 DAOMTA010000000
VERSION     DAOMTA010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_3794_cov_2.785504"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..707
                     /locus_tag="PLK68_06830"
     CDS             201..707
                     /locus_tag="PLK68_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRM90160.1"
                     /translation="MKIAIVFDSHTGNTKKVANVIKEACINEEVVYFGEPQTFSDADL
                     IFIGSWTDKGNCSQKIQNFITTLSNEKIAFFATAGFGGSTEYYDKLAERFDNVVNTNN
                     KILGHFFCPGKMPLSIRDRYVKMIQEHPEDKQLQVSIDNFDAALSHPDTNDLENAKKY
                     ALEMIAKV"
     gene            798..1670
                     /locus_tag="PLK68_06835"
     CDS             798..1670
                     /locus_tag="PLK68_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM90161.1"
                     /translation="MLNGKELLDNNTHIIYEKITTDELPLKVIEVTLAKDDPNLIPPK
                     HWHRSLEFIIPLTASIELWSNGETYSIARNGLAIINSQAIHTTKIIESEDCFKSIVIQ
                     IKYDFLKKCYPAFDNIYFSNHIVPEIEQKLVVLLTNLSLEYQQTTEFKLLTINGYLYL
                     IISILLKYQKKIRKGGYFLQSDQKLQEFMKILFYLDEHYSQALDVSSIANQFNFSYGY
                     LARLFKKHLNITVKQYLTAQRLEHCTNDLIHTNLTITEIAMKNGFSSTKSFNREFKLK
                     YHETPQVYRNKVRK"
     gene            1750..3072
                     /locus_tag="PLK68_06840"
     CDS             1750..3072
                     /locus_tag="PLK68_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90162.1"
                     /translation="MKRLSKLLLALFIACSITGCSQSTEVVDATYEIYIAGYDWGCGV
                     NKTILTLDKAVDDVDKNDFMVSETKQVTDWEDEALPVVEKTLERVVDDAYSCDKDGQK
                     IDGESKYIAIELYVSPNDGSPLLYSATTHYNTWSDPYYLNISLAKNGEITVDDKKVTK
                     LDVSTEYTKKITAADALESEKFKASDGIELNYGHFNPKEPSNTLFVWLHGSGEGGTED
                     TNPQVTSLSNKVSAYFNDDFQNAVGNAYVLVPQCPTFWMDADGNGGEWNDDLLVTHGP
                     SYYTNALMELIKDYQSRCGATKVVLAGCSAGGYMTMRLILDYPDYFAAAIPICEALPD
                     DVISDQQLSEIKDLPLYFIYSNDDPSIIPEKFEIPTINRLKNLGATNLHVATFDSVID
                     TSGKYNDLNDGDPYQYNGHWSWIYFDNNEADCDEHGEKVWQWIGEQVK"
     gene            complement(3210..3350)
                     /locus_tag="PLK68_06845"
     CDS             complement(3210..3350)
                     /locus_tag="PLK68_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90163.1"
                     /translation="MFKTGISSGREEFAHDILKGFVSVKSGFLSNKQEYLDLSNIHIS
                     KD"
     gene            complement(3414..>3794)
                     /locus_tag="PLK68_06850"
     CDS             complement(3414..>3794)
                     /locus_tag="PLK68_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HRM90164.1"
                     /translation="EGKTVSSRPNSHDYQYQENEYLYIDTFVGSEFFGGEECVYKDNI
                     PLYVMNYYGKVLDEAFSGDFLKEALLLVDRISPFRGPALYTNGNYTYHCSYDGDYEFF
                     NGKEEIYYNNIKVYECLFHGGSVK"
BASE COUNT         1346 a          549 c          579 g         1320 t
ORIGIN      
        1 gatacttttc aaatacttta ataaaagttc ttttaattaa ttcatctgta tataaaactg
       61 ttccatctaa atcaaatatt attgctgtat ccataaaatc tcctttacac atcatagtat
      121 atcacacgta attaaatgtg ataaagatta tgctttatta tttatgaata taagtctata
      181 cttatgacga ggtgattaaa atgaaaattg caattgtatt tgatagtcat acaggtaata
      241 ccaaaaaagt agctaatgta attaaagaag cttgtattaa cgaagaggtt gtttacttcg
      301 gtgaacctca aacattcagt gacgcagatc tgatttttat tggaagctgg accgataagg
      361 gtaactgtag tcaaaagatt caaaatttta ttactactct ttcaaatgaa aaaatcgctt
      421 tttttgcaac agctggattt ggtggttcta ctgaatacta tgataaactg gcagaacgat
      481 ttgataatgt tgtaaatact aataataaga tattaggaca ctttttctgt cccggtaaga
      541 tgcctttatc aatacgtgat cgctatgtta aaatgattca agagcatcct gaagataaac
      601 agcttcaagt tagtattgat aactttgatg cagctctatc acatccagat acaaatgatt
      661 tagaaaatgc taaaaaatat gcattagaga tgatagctaa ggtttaaata tcttagctat
      721 tttatatatt aaaataaatg aattggatta ataaaattaa tttttttatg ttattataat
      781 ataaaaggag tggtaggatg ttgaatggaa aagaattatt agataataat acgcatataa
      841 tttatgaaaa aattactact gatgaacttc cgctaaaagt tattgaggta actttagcta
      901 aagatgatcc taatttaatc cctccaaaac attggcatcg tagtttagag tttattattc
      961 cacttaccgc atcaattgaa ctttggagta atggggaaac ttattcaatt gctcgtaatg
     1021 gtttagctat cattaattca caagctatac atacgactaa aattattgaa tcagaggatt
     1081 gttttaaaag tattgttatt caaattaagt atgattttct aaaaaaatgc tatcctgcat
     1141 ttgataatat ctatttttct aatcatatag tcccagaaat tgaacaaaaa ctggtcgttc
     1201 ttttaactaa tttgagttta gagtatcaac aaactactga atttaaatta cttactatta
     1261 atggttattt atatttaatt attagtattt tattaaagta tcaaaaaaag attagaaaag
     1321 gtggttattt tcttcaatct gatcaaaaac tccaagaatt tatgaaaatt ctcttttatt
     1381 tagatgaaca ttattctcaa gcactagatg tttcctcaat cgcgaaccaa tttaacttct
     1441 cttatggtta tttagcaaga ttgttcaaaa aacacttaaa tattacggtc aaacaatact
     1501 taactgcaca acgtttagaa cactgtacta acgatttgat tcatactaat ttaaccatta
     1561 ctgagattgc gatgaaaaat ggcttctcta gtactaaatc ttttaatcgt gaatttaaac
     1621 tcaaatatca tgaaacaccc caagtatatc gtaataaagt tagaaaatga ccctaatgtg
     1681 gtcatttttt tcatatttaa attttataaa taagcttaag atataggcaa ggtgaaagga
     1741 gaagagagaa tgaaaagatt atcaaaatta ttacttgcct tatttattgc ttgtagcatt
     1801 accggatgtt ctcaaagcac agaagttgtt gatgcaactt atgaaattta tattgccgga
     1861 tatgattggg gatgtggtgt caataaaact attttgacat tagacaaagc tgttgatgat
     1921 gttgacaaaa atgattttat ggtatctgaa acaaaacaag taactgattg ggaggatgag
     1981 gcactcccag ttgttgaaaa aactttggag cgtgttgttg atgatgccta ttcttgtgat
     2041 aaagatgggc aaaaaattga tggagagtct aaatatattg cgattgagtt atatgtaagc
     2101 ccaaatgatg gttcgccatt attatactct gctacaacac attataatac atggagtgat
     2161 ccgtattatt taaatatttc attagcaaaa aacggtgaaa taacggtaga cgataaaaaa
     2221 gttactaaac ttgatgttag tacagaatat actaaaaaaa ttactgctgc tgacgcttta
     2281 gaatcagaaa aatttaaggc tagtgatgga attgaattaa attatggtca ttttaaccct
     2341 aaagaacctt ctaatacatt atttgtgtgg ttacatggtt ctggtgaagg gggaacagag
     2401 gatactaatc cacaagtaac ctctttatca aataaagtaa gtgcttattt taatgatgat
     2461 ttccaaaatg ctgttggcaa tgcatatgtt ttagttcctc aatgtccaac tttttggatg
     2521 gatgctgatg gtaatggcgg tgaatggaat gatgacttat tagtaactca tggtccttct
     2581 tattatacta atgcacttat ggaattaatt aaagattatc agtctcgatg cggagctact
     2641 aaagtcgttc ttgcaggatg ttctgcaggt ggatatatga ctatgcgttt aattttagat
     2701 tatccagatt attttgctgc agcaatacca atttgtgaag ccctgcctga tgatgttatt
     2761 agtgaccaac aactttctga aattaaagat ctacctctat attttattta ttctaatgat
     2821 gatcctagta ttattccaga aaaatttgaa attcctacta ttaaccgatt aaaaaatctt
     2881 ggtgcaacta atctacacgt tgccacattt gattcggtca tcgatacttc tggtaaatat
     2941 aatgatttaa atgatggtga cccataccaa tataatggtc actggtcatg gatctatttt
     3001 gataataatg aagccgactg tgatgagcat ggtgaaaaag tttggcaatg gattggtgaa
     3061 caagttaaat aaaatgaata taaaagatgt aacggataaa tttttaaacg ttacatcttt
     3121 taattataat tttcagttaa gttgacaatt atagggttcc tcatttaaat atttttattg
     3181 ctaaaaagtc tttctaattc ccctattttt tagtcttttg aaatatgaat attagataaa
     3241 tctaaatact cctgcttatt ggaaagaaat cctgatttaa ctgaaacaaa accttttaaa
     3301 atatcatggg caaattcctc tctgccacta ctgatacctg ttttaaacac ctaattaata
     3361 ttctaaaatc attttcttat tccagtatta attaaccgat tgatgtcatt taattattta
     3421 acacttccgc catggaataa acactcgtat actttaatat tattatagta tatttcctct
     3481 ttaccattaa aaaattcata atcaccatca taactacaat gatatgtata attaccatta
     3541 gtatacaacg ccggacctct aaaaggagaa attcgatcaa ctaataatag tgcttccttc
     3601 aaaaaatcac cactaaaagc ctcatcaagc actttaccat aataattcat tacataaagt
     3661 ggaatattgt ctttatagac acattcttca ccaccaaaaa attcactgcc tacaaacgta
     3721 tcaatatata aatattcatt ttcttgatat tgataatcat ggctgttagg tcgactggaa
     3781 acagttttcc cctc
//
LOCUS       DAOMTA010000209         3780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_832_length_3780_cov_2.454497, whole genome shotgun sequence.
ACCESSION   DAOMTA010000209 DAOMTA010000000
VERSION     DAOMTA010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_3780_cov_2.454497"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLK68_06855"
     CDS             <1..552
                     /locus_tag="PLK68_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM90165.1"
                     /translation="TDTVCVIGAGNVSMDCARTAKRLGAKNVYIVYRRSDKEIPARAE
                     EVHHAKEEGIIFKLLTNPVEIHGEDGWVKSMECVEMELGEPDESGRRRPVVKEGSNFV
                     IETGTVIVSIGQSPNPLIRQTTPGLETQKWGGIIVDEDSMKTSKEGVYAGGDVVTGAA
                     TVILAMGAGKTAAKAMDEYLANK"
     gene            634..1965
                     /locus_tag="PLK68_06860"
     CDS             634..1965
                     /locus_tag="PLK68_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRM90166.1"
                     /translation="MEQSITFVDVETPNYQNNSISSIGVINVDGDGVVTTKYFLVDPE
                     AHFDRFNIELTGITPEMVADQPNFKEVWSEIEPYFTNSLVVAHNAVFDLSVISACLQR
                     YDLPIFPIFYTCTYRISRALKIPSNSYKLNDLSSYYHVTLDNHHNALADSKACMEIFY
                     YLLKEPNLETLDQYVKCFEPTKGNKDNKKYLEVLIGLLTGIGFDNYLNKKEISFLNNW
                     LTKNQLPYEYANIVKELKAVLKNEYITHYQYLHILNELQYMKSIKAKNIRSLYEFMAI
                     LEGISCDEVINDDEIMELNKWMKENEQFKGSYPFNRILNKLEKIIIDKQISTIVTDEL
                     LYYIKNFFKPELDQGDLFDVKNKVICLTGNFCFGERSQLEKLIVLKQGIISKSVTKKV
                     DYLVLGSKGSAGYKYGKYGAKTNKALTMKSEGHKIELISEARLMEVLKLSK"
     gene            1984..2568
                     /locus_tag="PLK68_06865"
     CDS             1984..2568
                     /locus_tag="PLK68_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM90167.1"
                     /translation="MKVLVIYCHPSNKSFTARMKDEFIRGLEDGGHTCQLIDLYQINF
                     DETFSEEEYLREAFYDQALKVPKDVQAHQRLINANDAVVFIYPVFWTEAPGKLVGWFQ
                     RVWTYGFAYGTETKMKQLDKVLMLVTMGGDLSEEIRQQQVAAMKVVMLGDRIGERAKT
                     KEMIVFDRMSRDYPVREVNYSKNLKRAYLLGKEF"
     gene            2573..3115
                     /locus_tag="PLK68_06870"
     CDS             2573..3115
                     /locus_tag="PLK68_06870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRM90168.1"
                     /translation="MYKERVKENLETTRLRLRKFKLSDKEAVYKYGRDPRTLKYLEWI
                     GVTTREEARISIEEYYLSRAGIYAIALKENDLCIGAIDIRLDEANDKASFGYLLDPDY
                     WNQGYMSEVLSRILKHCFEDIRVNRVEAIHYRLNPASGKVMAKCGMKQEGIGIQELKI
                     KGLYHDVVHYAITQEMWQRR"
     gene            3221..>3780
                     /locus_tag="PLK68_06875"
     CDS             3221..>3780
                     /locus_tag="PLK68_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90169.1"
                     /translation="MSLSDNLRALRKQKGYSQEQLAERLNVSRQAVSKWESDNGYPEM
                     ESLIILSDLFECTIDDLLKNDLTQHNPTAKQAYDKHYSLIAKAYTFGVVSILLGVCAY
                     LFAEIYFSENTKSEFIGEIIFLFFVLIGVITFVYFGMKDSHFKNSHSEITDYYTDSQR
                     SEFNQTYSLSIATGVGVILFAVVLQI"
BASE COUNT         1351 a          440 c          793 g         1196 t
ORIGIN      
        1 actgatactg tatgtgtaat tggggctggt aacgtttcaa tggactgtgc tcgtacagca
       61 aaacgtttag gtgcaaaaaa tgtttatatt gtttatcgcc gtagtgataa agaaattcca
      121 gctcgtgctg aagaagtaca tcatgctaaa gaagaaggca ttatttttaa attattaact
      181 aacccagttg aaattcatgg tgaagatgga tgggtcaaat caatggaatg tgttgaaatg
      241 gaacttggag aacctgatga atcaggaaga agaagaccag ttgttaaaga aggatcgaat
      301 tttgtgattg aaaccggaac tgtaattgtt tcaatcggac agagtcctaa tccgttgatt
      361 cgtcaaacca caccaggatt agaaactcaa aaatggggtg gaatcatcgt tgatgaagat
      421 tctatgaaaa caagtaaaga gggcgtatat gctggtggtg acgttgttac tggagcagct
      481 acagtaattc tagcaatggg agctggtaaa acagctgcta aagcaatgga tgaatatctt
      541 gccaataaat aattgaattc ctaggtaatc cctaggaatt tttttaaaat aaaatggttt
      601 tcttgttata atggtaaata ggaggagcaa aatatggaac aaagtataac atttgtggat
      661 gtagaaacac ctaattatca aaataatagt attagttcaa ttggtgttat caatgttgat
      721 ggtgatggtg ttgtaacaac taaatatttt cttgttgatc ccgaggcaca ttttgaccgt
      781 ttcaatatag aattaacggg aataacccca gaaatggttg ctgaccaacc aaatttcaaa
      841 gaagtgtggt ctgaaataga accatatttt acaaatagtt tagtggttgc ccataatgct
      901 gtttttgatt taagtgttat cagtgcttgt ctgcaacgtt atgatctgcc tatcttccct
      961 attttttata catgtactta tcgaatcagt cgcgctttaa agattccatc caatagttat
     1021 aaattaaatg atttaagtag ttattatcat gtaaccttgg ataatcatca taatgcttta
     1081 gcggatagta aagcatgtat ggaaatattt tattatttat tgaaagaacc aaatttggaa
     1141 actttagatc agtatgttaa atgctttgaa ccaaccaaag gaaataaaga taataaaaaa
     1201 tatttagagg tactgattgg tcttttaacc ggaattggat ttgataacta tttaaataaa
     1261 aaagaaatta gttttttaaa taactggtta actaagaatc agcttcctta tgagtatgct
     1321 aatattgtca aggaattaaa agctgttttg aaaaatgaat atataacaca ttatcaatat
     1381 ttacatattc ttaatgaact ccaatatatg aaaagtatta aagctaaaaa tatacgttca
     1441 ttatatgagt ttatggctat tttggaaggg atcagttgtg atgaagttat taatgatgat
     1501 gaaattatgg agttaaataa gtggatgaaa gaaaatgaac aatttaaagg ttcttatccc
     1561 ttcaaccgaa ttttaaataa attagagaag attatcattg ataaacaaat aagtacaata
     1621 gttacagatg agttgttata ttatatcaaa aatttcttta aaccagagct tgatcaaggt
     1681 gatttatttg atgtcaagaa taaagttatt tgtttaaccg gtaatttttg ttttggggaa
     1741 cgaagtcaat tagaaaaact tattgttcta aaacaaggaa ttataagtaa aagtgtgaca
     1801 aaaaaagttg attacctggt tttaggaagc aaagggtcag ctggatataa atatgggaaa
     1861 tatggtgcta aaacaaataa ggctttgaca atgaaaagtg aaggtcataa gattgaatta
     1921 attagtgaag ccagattaat ggaagttttg aaactttcta aataaattga tgtgaggtag
     1981 atgatgaaag tattggtgat ttattgtcat cccagcaata aaagttttac agctaggatg
     2041 aaagatgagt ttattagagg attggaagac ggagggcata cttgtcaact tattgatttg
     2101 tatcaaatta attttgatga aacatttagt gaagaagaat acttaagaga agccttttat
     2161 gatcaagcct taaaagtgcc taaggatgtt caagcacatc aaagactaat taatgcaaat
     2221 gatgcggttg tgtttattta tcctgttttt tggaccgagg caccgggaaa actggttggg
     2281 tggtttcaac gcgtttggac ttatggtttt gcttatggga ctgagactaa aatgaaacag
     2341 ttagataaag tactgatgct tgtcacgatg ggtggcgacc taagtgaaga aattcgtcag
     2401 cagcaggttg cagcaatgaa agttgtgatg ttaggagatc gaattggtga acgtgccaaa
     2461 acgaaggaaa tgatcgtgtt tgaccgtatg tcccgagatt atccggtacg tgaagtaaat
     2521 tattcgaaaa acttaaaacg tgcctactta ttaggaaagg aattttagaa gaatgtataa
     2581 agaacgggtc aaagaaaatt tagaaaccac aagattacga ttaagaaagt ttaaattaag
     2641 tgataaagaa gcagtttata aatatggaag agatccgcga actctaaaat atcttgaatg
     2701 gattggggtg actactcgtg aggaagctag aataagcatt gaagaatatt atttaagtcg
     2761 tgcaggaatt tatgcaattg ctttgaaaga aaatgattta tgtattggag cgattgatat
     2821 tcgtttagat gaagctaatg ataaagctag ttttgggtac ttattggatc ccgattactg
     2881 gaatcaagga tatatgagtg aggtattatc acgaatttta aaacactgtt ttgaagacat
     2941 aagagtaaac cgggttgaag caattcatta tagattaaat ccggcctctg ggaaagtgat
     3001 ggcaaaatgt ggaatgaagc aagaaggaat aggaattcaa gagctaaaaa taaaaggcct
     3061 ttatcatgat gtggtgcatt acgcgataac tcaagaaatg tggcaaagac gttgatatca
     3121 cgttattgca acgcaaagtt gacaaaaata attaaagtgc aatggcaagt tggtagtaaa
     3181 aaagtaactg cataattatg atttgggtga ggtgataaag atgagtttaa gtgataactt
     3241 aagagcttta agaaaacaaa aggggtattc acaagaacaa ttggcagagc gtttaaatgt
     3301 ttctaggcaa gctgtttcga aatgggaaag tgataatggt tatccagaga tggaatcgtt
     3361 aattatttta agtgacctgt ttgaatgtac tattgatgat ttattgaaaa atgatttgac
     3421 tcaacataat ccaacagcca aacaagcgta tgataaacat tatagtctaa tagcaaaggc
     3481 ctatacattt ggagttgttt caatcttgct gggggtatgc gcatatctat ttgctgaaat
     3541 ttatttttcg gaaaatacaa aatctgagtt tattggcgaa attattttct tgttttttgt
     3601 tttaattggg gtaattacat ttgtttattt tggaatgaaa gatagtcatt ttaaaaatag
     3661 tcattctgaa ataacagatt attatacgga tagtcaaaga agtgaattta atcaaacata
     3721 ttcattgagt atcgcaactg gggtaggagt gattttattt gcagttgttt tacagatttt
//
LOCUS       DAOMTA010000210         3777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_833_length_3777_cov_2.599946, whole genome shotgun sequence.
ACCESSION   DAOMTA010000210 DAOMTA010000000
VERSION     DAOMTA010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_833_length_3777_cov_2.599946"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLK68_06880"
     CDS             complement(<1..221)
                     /locus_tag="PLK68_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM90170.1"
                     /translation="MKKNKNNRKKYIVGVVLIFLGCLIVTSIIWGNRTFSVKTLNQII
                     FHLKVPMEGTDNGIYLDWFIWAVPISIVAG"
     gene            complement(310..1152)
                     /gene="rfbD"
                     /locus_tag="PLK68_06885"
     CDS             complement(310..1152)
                     /gene="rfbD"
                     /locus_tag="PLK68_06885"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRM90171.1"
                     /translation="MKILVTGVNGQLGYDIVKECQKRNIEAVGVDVDEMDITNAKQVD
                     KVIKKNKYDAVVHCAAWTAVDKAEDEIDACTKVNVVGTKNIVKVCKELDIPLMYFSTD
                     YVFDGQGESEWKEYDERHPLNVYGQTKYEGELAVQTLNRYFIVRIAWVFGVNGNNFIK
                     TMLRLGKAHGAVSVVNDQIGSPTYTYDLAKLCVQMIQTNKYGIYHATNEGLCSWYEFA
                     CEIFKQAGMNVEVTPVDSTKFPAKAKRPNNSRMSKTMLDKNGFDRLPTWQDALSRYLK
                     EIYD"
     gene            complement(1246..3105)
                     /locus_tag="PLK68_06890"
     CDS             complement(1246..3105)
                     /locus_tag="PLK68_06890"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HRM90172.1"
                     /translation="MLENKTKKLIIPIIFIIISIVLELFLSNYKVFMLNDSQKGNHII
                     NNNIQIDEKNIDGENVIDIRISDIDSYIKKFKVSYKAKVNTSISLTINVSNEYNKITE
                     VHQNETILKDLNESVINISKNVKSIVINNLNEKNVEQLKFSYNNNYTFSIYRWLFFIV
                     TLMLCYIVFINRKKASEKLHRTCFLTIISIGMLFIIGSPKIANMNFDEQVHVYLAFTN
                     SYGSEVSYSDAFYYVSESPGIMLPKSFEGNKELNKLLNSNQMAEKNTTTKSKFVKYNE
                     IPYLVSSFGIKLANVLSLPFTFVFCLGKVMNLLAYAVMASYAVKKSKCGKVLVYVLAL
                     LPTTIYAASHYSLDAIVSGGVLLSICTFVEMYRKKNEKISIKELIIFIFPLILACLAK
                     AIYAPALLLLLLLPTTKFNNKRQSMLFKGTVCFMFIMMISSFLLPMLSGGIQGDVRGG
                     NTSVIEQLKLILSNPAGFAQMFYRNIIDTVYKNFFSFQIFNNFAYVPSGNNIYTVIYV
                     VALLYAAFISGKDYIFIGKKERIIILVGVMTTICMVYGSMYLSFTEVGATEIAGVQAR
                     YFIPVLFPLILTLVPRNQINNLNTDQQITIINYMLLVVLLVTIYLSFIIVYCI"
     gene            complement(3092..>3777)
                     /locus_tag="PLK68_06895"
     CDS             complement(3092..>3777)
                     /locus_tag="PLK68_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90173.1"
                     /translation="MLLDWIKKMKRIKKYKLLMLKLYSVFLLIVVCFCIFLSIYWYIK
                     PRTNDVGENHIYSEEFVNSDTIKLYDNLEFQMKTTLNVSKVANIGVYSSRLGDADKDS
                     KVKMVLQEKGGKILYAMEYNFKDIQEKSVLAIFPNNLNDMLGKEVKLTITTHGVSATN
                     PLILHKTNIENGAKYYLNGEKQQDMYLAYLITGSSPSYFFIWYPMMLLSIVIVLCSVN
                     DWSVLYARK"
BASE COUNT         1318 a          651 c          379 g         1429 t
ORIGIN      
        1 cctgcaacaa tactaatagg taccgcccaa atgaaccagt ctaaataaat tccattgtct
       61 gtgccttcca taggaacttt taaatgaaat ataatttgat ttaatgtttt tactgaaaaa
      121 gtacgattgc cccatattat tgatgttaca attagacagc ctaaaaaaat tagaacaacg
      181 cctactatat acttttttct attgttttta ttttttttca tatgtacctc ataaataatt
      241 cctttctatt ttatcatatc taactctaat aataaacaaa agaaaaagat tgaaataatc
      301 actattattt caatcatata tttcttttaa atatctactt aaggcatctt gccaagttgg
      361 caaacgatca aaaccatttt tatctaacat tgttttggac attctactat tatttggtct
      421 tttagcttta gctgggaatt tagtagaatc aacaggtgtt acttccacat tcattcctgc
      481 ttgtttaaaa atttcacaag caaattcata ccatgaacat aatccttcat tagtagcatg
      541 ataaattcca tatttatttg tttgaatcat ttgtacacat aatttagcta aatcatatgt
      601 atacgttggt gagccaattt gatcatttac aacactaacc gcaccatgtg ctttacccaa
      661 acgaagcata gttttaataa aattgttacc attaacgcca aatacccatg caatacgaac
      721 aataaaatat ctattcaaag tttgtacagc caattcgcct tcatatttag tttggccata
      781 cacatttaac ggatgtcttt catcatattc tttccattcc gactctcctt gaccatcaaa
      841 cacataatca gtagaaaaat acattaatgg aatatctaat tctttacata ctttcacaat
      901 attttttgtt cccacaacat taactttagt acaagcatca atttcatctt cagctttatc
      961 caccgctgtc catgctgcac aatgcactac tgcatcatat ttattttttt taattacttt
     1021 atcgacctgt ttggcattag ttatatccat ttcatcaaca tcaacaccaa ccgcttcaat
     1081 atttctcttt tgacattctt ttacaatatc gtaacccaat tgaccattaa ctccggtaac
     1141 cagtatcttc attctatatc tccttaattt tcttaaaaaa tagcctaacc attaaataat
     1201 atagtataat ttttattata tttctttgct atacagtatt gtattttata tacaataaac
     1261 aattataaaa cttaaatata ttgtaactag taatacaaca agtaacatat aatttattat
     1321 tgttatttgt tgatcagtgt ttaaattatt aatttgattt cttggtacta aagttagtat
     1381 taacggaaat aaaacaggta taaaatatct agcttggact cctgcaattt ctgttgcccc
     1441 aacttcagta aatgataaat acattgatcc ataaaccata catattgtag tcataacacc
     1501 tactaaaatg ataattcgtt cttttttacc aataaatata taatcttttc ctgatataaa
     1561 tgctgcatat aataatgcca ccacatatat tactgtataa atattgttac cagatggtac
     1621 atatgcaaaa ttattaaaaa tttgaaatga gaagaaattt ttatagacag tatcaataat
     1681 atttctatag aacatctgtg caaaaccagc tggattggac agtataagct ttaattgttc
     1741 tataacactt gtatttcccc cacgtacatc accttgtatt ccaccgctta acataggtaa
     1801 taagaaacta ctaatcatca taataaacat gaaacaaact gtccctttaa ataacatact
     1861 ttgccgttta ttattaaatt ttgttgtagg tagaagaagt agcaataata atgctggcgc
     1921 atatattgct tttgcaaggc atgctaaaat caaaggaaaa ataaatatga tcaattcctt
     1981 tatacttatt ttttcatttt tctttctgta catttcaaca aatgtacata ttgatagtaa
     2041 gacacctcca gaaactatcg catctaatga gtaatgactg gctgcataaa tcgtagtagg
     2101 taataatgct aaaacataca ctaatacttt accacattta gattttttta ctgcataact
     2161 tgccataact gcataagcta aaaggttcat aaccttccct aaacaaaaaa caaatgtaaa
     2221 tggtaaactt aaaacattgg ctagtttaat tccaaaactc gatactaaat aaggaatttc
     2281 gttatattta acaaactttg attttgtagt agtatttttt tctgccattt gattactatt
     2341 aagtaattta tttaattcct tatttccttc aaatgacttt gggagcatta tccctggaga
     2401 ctctgaaaca taataaaacg catcactata agatacttca ctaccataac tatttgtaaa
     2461 cgctaaataa acatgtacct gttcatcaaa attcatattt gctatcttag gagatccaat
     2521 tataaataac attccaattg aaattattgt taaaaaacaa gttctatgta atttttcaga
     2581 tgcttttttt ctatttatga acactatata acacaacatc agtgtgacta taaaaaacag
     2641 ccatctgtaa atcgaaaaag tatagttatt gttataacta aattttaatt gttctacatt
     2701 tttttcattc agattattaa taactattga tttaacattc ttactaatat ttattacact
     2761 ttcattaaga tcttttaata ttgtttcatt ttggtgaact tctgtaattt tattatattc
     2821 gtttgataca tttatagtaa gagatattga tgtgtttact ttagccttgt atgacacctt
     2881 aaattttttg atataagaat caatatcact tattcttata tctattacat tttcaccatc
     2941 aatgttcttt tcatctattt gaatattatt atttataata tgattgcctt tttgtgaatc
     3001 atttaacata aaaactttat aattgcttaa aaacaactct aatacaatcg atataataat
     3061 aaatatgatt ggtataatta attttttcgt tttattttct agcatataat acactccaat
     3121 catttactga acataataca ataactatag ataataacat cattggatac catataaaaa
     3181 aatatgaagg gctactgcca gttattaaat atgcaagata catatcttgc tgtttttccc
     3241 catttaagta atatttcgca ccattttcta tatttgtctt atgtaatatc aatggattag
     3301 tcgcgctaac tccatgcgta gtaattgtca attttacttc ttttcctaac atatcattta
     3361 aattattagg aaatattgct aaaaccgatt tttcctgtat atctttaaaa ttatattcca
     3421 tagcatacag aatcttgccc cctttttctt gcaataccat ttttacttta ctatctttat
     3481 ccgcatcacc caacctagaa ctgtatacac caatattcgc cactttgctc acatttaatg
     3541 tagttttcat ctgaaattca agattgtcat acaatttaat tgtatcacta tttacaaatt
     3601 cctctgaata tatatgattt tctccaacgt catttgtcct aggtttaata taccaataaa
     3661 tacttaggaa tatacaaaaa cacactacta tcaataaaaa taccgaatac aattttaaca
     3721 ttaataattt atacttttta attctcttca ttttttttat ccaatctaaa agcatta
//
LOCUS       DAOMTA010000211         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_834_length_3776_cov_1.946520, whole genome shotgun sequence.
ACCESSION   DAOMTA010000211 DAOMTA010000000
VERSION     DAOMTA010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_834_length_3776_cov_1.946520"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLK68_06900"
     CDS             complement(<1..605)
                     /locus_tag="PLK68_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRM90174.1"
                     /translation="MKELVMIDGNSLLYRAYYATAAMGNLMVNKDGVPTNAVYGFANM
                     LESILKGNPEYLVVAFDYGKKTFRNDLFEVYKGTRSATPDELACQFSMIREYLTAHGI
                     RYQEIEGYEGDDIIGTVAVKASKQRFKVSIVTSDKDMLQLVDDNISVYLTKKGVGELE
                     KITPAKFKEIYGLIPDQMRDLKGLMGDKSDNIPGIPGVGEKT"
     gene            complement(617..3670)
                     /gene="dnaE"
                     /locus_tag="PLK68_06905"
     CDS             complement(617..3670)
                     /gene="dnaE"
                     /locus_tag="PLK68_06905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRM90175.1"
                     /translation="MLGHLQVYSSYSFQNSTILIEDLVKKASDLHYEALALTDKDNMY
                     GAMELAKYAKKYGIKAVFGLEASVEINHEIYPLLLLARDVTGYFDLVKITSEICLSER
                     GAISLEALSLYREHLFVISACKEGIIERLILKELDSEALNYLELFKNTFNNFYVGLQN
                     HGIAMQQKLNERLVALATLQNIPICVTNEVRYLNKDEAFTLDLLQASAKGMMLDLEHQ
                     VQTDQLYLKSSFEMESLFDKKYIENTNDIIRLCNVTIPTDQMHLPKYPVPKDGDAGDY
                     LRQLCLVGLKKRFKGKNIPNEYIERLKKELTVINKMGFNDYFLIVFDYVRYAKMHKIL
                     VGPGRGSAAGSLVAYVLGITGVDPLRYDLLFERFLNEERISMPDIDIDFQDDRRDEVV
                     NYVTEKYGHEHVAQIVTFSTYGPKVAIKDLGKVLGVPLPRLELLTKNIPTNYKNRKSA
                     REVFETSYSFQSMVNKDPALRKIMPAVFIVEKLPRNISTHAAGVVLSNDVLDQVVPLV
                     RGPNNGVVTQYSKDYIEEVGLLKMDFLGLKNLTIIDYIIKDIAKNHQVEININDIDLQ
                     DKKTYEMISRGDTFGIFQLESSGMKNLLVKMQCDCLDDVIAAIALFRPGPMANIPSYI
                     ARKKGKEPITYPLECLKPILKSTYGILLYQEQIMQAAQLVAGFSLAKADILRKAMSKK
                     TASLMKAMKSEFIQGGIANGFSELEAVKVFELIERFANYGFNKSHSVVYGYIAYWLAY
                     LKANFPLEFFSALLSNEQSSDASKLSCIQEGKKYGVKLLAPSINYSTDRFKVEDGNIR
                     YSLLAIKNVGYAGYKAIEAERQNGTFKDIFDFVSRMESSKLNSKMLDSLIMAGAFDEF
                     KLNRTTIKQNLHKIMEYAELKNSIGIDEPPILTIVRDNRIKVLEEEKMVLGVYLTMHP
                     IALIKNNLKEQIVNLNELSNYIDQPVRVIMALSRVKVIVDKKGQEMCFVDGYDETGEV
                     DAVVFASSFQVLKKILVRGEICLIEGRVNYRDKLSLIINQAKNVR"
BASE COUNT         1242 a          745 c          471 g         1318 t
ORIGIN      
        1 gttttttctc caactccggg aatccctggg atattatcag atttatcacc catcaatcct
       61 tttaaatcgc gcatttgatc aggaatcaaa ccatatattt ctttaaattt agcaggggtg
      121 attttttcta attctcctac ccccttctta gtaagataaa cactaatatt atcatcaact
      181 aattggagca tgtctttatc acttgtaaca atacttactt taaaacgttg tttgcttgct
      241 ttaacagcaa cagttccaat aatatcatcg ccttcatacc cttcgatttc ctgataacga
      301 attccatgag ctgttaaata ttcacgaatc attgaaaact gacaagctaa ttcatcagga
      361 gtggcgctac gagttccttt ataaacttca aataaatcat ttctaaaagt ttttttaccg
      421 taatcaaagg caacaactaa atactcagga ttacccttta aaatactttc taacatattg
      481 gcaaatccat acactgcatt agtaggaaca ccatctttat ttaccattaa atttcccatt
      541 gctgccgttg cataataagc acggtacaac aacgagtttc catcaatcat tactaattct
      601 ttcatttcta ccttccttaa cgtacatttt ttgcttgatt tattattagt gatagtttat
      661 cacgataatt aactctacct tcaattaaac aaatctcacc acgtactaat attttcttta
      721 aaacttgaaa acttgaagca aagacaacag catctacttc accagtttca tcgtaaccat
      781 caacaaagca catctcttgt ccttttttat caacaatcac ttttactctc gataatgcca
      841 taataactct aaccggctga tcaatataat tggataattc atttaaatta actatttgct
      901 cctttaagtt attcttgatc aaagcaatcg gatgcattgt caaatatact ccaaggacca
      961 tcttttcttc ttccaatact ttgatccgat tatcacgaac gatcgttagg atcggtggtt
     1021 catcgattcc aattgaattt ttcaattctg catattccat aatcttatgt aaattttgtt
     1081 taattgtcgt gcgatttaat ttaaactcat caaaagctcc tgccataatt aaactatcaa
     1141 gcattttact atttaatttg cttgattcca ttctagaaac aaaatcaaat atatctttaa
     1201 aagtaccatt ttgacgctct gcttcaattg ctttatatcc tgcataacca acatttttaa
     1261 tcgctaataa agaataacga atattgccat cttcaacttt aaagcggtca gttgaataat
     1321 tgattgatgg tgccaatagt ttaacaccat atttttttcc ctcttggata caactaagtt
     1381 tacttgcatc actactttgt tcatttgata ataaagcact aaaaaattct aatggaaagt
     1441 tcgcctttaa atatgctaac caataggcaa tatatccata gacaaccgaa tgtgatttat
     1501 taaaaccata gttagcaaac ctttcaatca attcaaacac tttaactgct tcgagctcac
     1561 taaacccatt agcaattccc ccttgaataa attcactctt catcgctttc attaaactcg
     1621 ctgtcttttt tgacattgct tttctcaaaa tatccgcttt agctaatgaa aatccagcaa
     1681 ctaattgtgc cgcctgcata atttgttctt ggtataaaag aattccatat gtagatttta
     1741 aaattggctt taaacattct aatggataag ttattggttc tttgcctttt tttcgagcga
     1801 tatatgaagg aatattagcc atcggtcctg gtctaaataa tgcgattgca gcaataacat
     1861 catcaagaca atcacattgc atcttaacta ataaattttt cattcctgag gactctaatt
     1921 gaaagattcc aaaagtatca ccacgactga tcatttcata agttttttta tcctgaagat
     1981 caatgtcgtt aatattaatt tcaacttgat gattcttagc aatatcttta ataatataat
     2041 caatgatcgt taaattcttt aaacctaaaa agtccatttt gagcaagccg acttcttcga
     2101 tataatcttt tgaatattga gttacaactc cattgttagg acctctgaca agcgggacaa
     2161 cttggtccaa tacgtcattt gataaaacca ctcccgctgc atgcgttgaa atattacgtg
     2221 gcaatttttc aacaataaaa acagcaggca tgatctttct taatgcagga tctttattca
     2281 ccattgattg aaaactatat gatgtttcaa aaacttctcg agcactctta cgattcttat
     2341 aattagtagg aatattttta gttaacaatt ctaatcgtgg caagggtacc cctagtactt
     2401 ttccaagatc tttaattgct actttaggac cataagtaga aaaagtaacg atttgcgcta
     2461 catgctcatg accatatttt tcagtaacgt aatttacaac ttcatcacga cggtcatctt
     2521 gaaaatcaat atcaatatca ggcatactaa ttctttcttc atttaaaaaa cgttcaaata
     2581 gtaagtcata tcgcaatgga tctactccag taattcccaa tacataagcc actaggctgc
     2641 cagccgctga accacgtcct gggccaacta agatcttatg cattttagca tatcgaacat
     2701 aatcaaaaac aattaaaaaa tagtcattaa atcccatctt attgattact gttaattctt
     2761 tttttaagcg ttcaatatat tcattaggaa tgttttttcc tttaaatcgt tttttcaaac
     2821 caactaaaca taattgcctt aagtaatcac cagcatcgcc atctttagga actgggtatt
     2881 taggtaaatg catttgatct gttggaatgg ttacattaca cagtcgtatt atatcgttag
     2941 tattttcaat atatttttta tcaaaaagac tctccatttc aaaactagat tttaaatata
     3001 attgatcagt ttgaacttgg tgttctaaat ctaacatcat tcctttagcg gaagcttgta
     3061 aaagatctaa agtaaaagcc tcatctttat ttaaataacg gacctcattg gttacacaaa
     3121 tgggaatatt ttggagagtt gctaaagcga ctaaacgttc atttaatttt tgttgcattg
     3181 caataccatg gttttgtaat cctacataaa aattattaaa agtgttttta aacagttcta
     3241 aataatttaa cgcctccgaa tccagttcct ttaggataag tcgttcaata attccttctt
     3301 tacatgctga aattacaaat agatgttcac gatataatga cagtgcctct aaactaatcg
     3361 cacctctttc actcaaacaa atttcactag ttattttaac caaatcaaaa taaccagtca
     3421 cgtcccttgc caatagtaat aacggatata tctcatgatt gatttcaaca cttgcttcta
     3481 acccaaagac tgctttaata ccgtattttt tagcatattt agctagttcc attgccccat
     3541 acatgttatc tttatcagtt aaagctagcg cctcatagtg gagatcacta gcttttttta
     3601 ccaagtcctc aattaagata gtactatttt gaaaactata tgaactataa acttgtaaat
     3661 gtcctagcat ctatccccac atcctttcta ttgtgtatta taccactaaa taagctattt
     3721 aaaaagatat tagaggattg cacggaacga aaaaagaata atcataatag aaaaat
//
LOCUS       DAOMTA010000212         3768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_840_length_3768_cov_2.056827, whole genome shotgun sequence.
ACCESSION   DAOMTA010000212 DAOMTA010000000
VERSION     DAOMTA010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_3768_cov_2.056827"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="PLK68_06910"
     CDS             <1..1642
                     /locus_tag="PLK68_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM90176.1"
                     /translation="KTRFSKFFNLPELMSMFREVADIQTADMLDLPTPKANYGVISVP
                     ASPLQKEMILALGERAEKIKSGNVDPHIDNMLKITSDGKKLALDQRLVNPLLPENKES
                     KVVACVNKAYDTWKDTKENLSTQLIFCDMSTPKISSKKINEESILEDTDIIFTSVYED
                     IKRKLVERGVPSHEVAFIHDTNNNDVLRKELFANVRSGKVRILLGSTSKMGAGSNFQD
                     RIITLHDLDCPWRPRDLEQRSGRAIRQGNINPEVNIIRYVTEGTFDAYMFQTIEKKQS
                     FISQVMTGKIIQRTMEEVDDMSMRYAEIKAIACGDPKIMERCDLDIEVSKLNDFFRNY
                     LNQKYELQNNILKKLPREIMKTEQTISDLKEDIVLRNQNPLPENDEFVGIKIDNVLYT
                     DKAEAGSMLIESVIKNLTSEPAYIGEYRSFPIYSHHIALTKEFYITLKNKASIMITVG
                     TDRLGNFIRMNNALKNLEKTLTESEQKLTLLHQELRTSKEEFEKPFPYEDELNEKTKR
                     LAQLTMELKLDEREPDVNDDSDMEEPEEDIIKEREYER"
     gene            1744..2739
                     /locus_tag="PLK68_06915"
     CDS             1744..2739
                     /locus_tag="PLK68_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 and toprim domain-containing protein"
                     /protein_id="HRM90177.1"
                     /translation="MSHIKPHELKEIKKIDLFTYLKNYQPDRLKKISHDTHCIRDHQS
                     LHISNGLWHWQSTGIGGRSALDFLIKVDNYSFLDAAMILLEKTKAKEPIYTAYSPKEE
                     NKKLQLPLASKTNEHALDYLMRRGIDREIIQDCIQNQLIYESKSRNTSTNKIYTNVTF
                     LGYDRSNHQPKYANIRNVDVDFKGDVAGSDKHFCFSIASQHLSNELHIFESAIDALSY
                     ATLLKMNEKNYKDIHLLSLGGVAIPRKNTSEQIKFPVALKQYLEDFPNINKVILHLDN
                     DRAGRIASQNIQKELWYMETEDIVPRYGKDVNDELKFRLGMPISLKNKYDRQKER"
     gene            2754..2960
                     /locus_tag="PLK68_06920"
     CDS             2754..2960
                     /locus_tag="PLK68_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90178.1"
                     /translation="MIKKIEKEIRRLELLKEQKTVKRNQLDEEISEASTQLKELYSLK
                     NQYEKLQNNTANFFEKNENTPKSF"
     gene            3245..>3768
                     /locus_tag="PLK68_06925"
     CDS             3245..>3768
                     /locus_tag="PLK68_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90179.1"
                     /translation="MNKTIEQLIENLDTIPFLFVGSGLSRRYYNLPDWIGLLKVMVDK
                     LNKDSFAFRSYEDRASFESNPYGINPKIASLIEEDFNKEWFQNPAIRNLDEIYLEKVE
                     KGCSPFKAEMSYYLKQKSVLHSNLKDEVTLLNNIAKKSIAGIITTNYDLFFETYLSEY
                     KVYIGQQALLFSQL"
BASE COUNT         1471 a          529 c          643 g         1125 t
ORIGIN      
        1 aaaaactcgt ttttccaaat tctttaatct tccagaattg atgagtatgt ttcgtgaagt
       61 ggcagatatt caaactgctg atatgttaga tttaccaaca ccaaaagcaa actatggtgt
      121 aatctcagtt ccagcaagtc ctctacaaaa agaaatgatt ttagcacttg gcgaacgagc
      181 tgaaaaaatc aagagtggaa atgttgatcc acatatagat aatatgctga aaattacttc
      241 tgatggaaag aaattagcac tagatcagcg tctagtcaat ccgcttttgc ctgaaaataa
      301 agaaagtaaa gtcgttgcat gtgtgaataa agcgtatgat acttggaaag atacgaaaga
      361 aaatctttcc acacagttaa tattctgtga tatgtctaca ccaaaaatat cttctaaaaa
      421 aatcaatgaa gaaagcattt tagaagatac tgacatcata tttacgagtg tttatgaaga
      481 tataaaacgt aagcttgtag agcgtggtgt tcctagtcat gaagttgcct ttattcacga
      541 tactaataat aacgatgttt taagaaaaga attatttgcg aatgtacgtt ctggcaaggt
      601 gcgtattctt ttaggttcaa cgagtaaaat gggtgccggt tcaaattttc aagatagaat
      661 aattacgctt catgatttag attgtccttg gcgaccacga gatttagaac agcgaagcgg
      721 tcgtgctatt cgtcagggaa atatcaatcc ggaagtcaat atcattcgtt atgtaacaga
      781 aggtactttt gatgcctaca tgtttcagac aatagagaaa aaacaatctt tcatctctca
      841 agtgatgact ggcaaaatta ttcagcgaac aatggaagaa gttgatgata tgtctatgcg
      901 atatgcagaa attaaagcaa ttgcctgcgg tgatccaaag attatggaac gatgtgattt
      961 agatatagag gtcagtaaat taaatgattt ctttagaaat tatcttaatc agaagtatga
     1021 acttcaaaac aatatcttaa agaaactgcc aagagaaatc atgaaaaccg aacaaacgat
     1081 aagtgattta aaggaagata ttgtattgag aaaccaaaat ccattaccag aaaatgatga
     1141 atttgttggt attaaaatag ataatgtcct atatacagat aaagcagaag cagggagtat
     1201 gctgattgaa tcggttataa aaaatttaac gagtgaacca gcatatatcg gtgaatatcg
     1261 ttcttttcct atatattcac atcacatagc tttaacaaag gagttttata taactctaaa
     1321 aaacaaagct tcaattatga ttacggttgg aacggataga ctgggaaatt tcatacgtat
     1381 gaacaatgct ctaaagaatt tagaaaaaac actaacagag tcagaacaaa aactaacact
     1441 tcttcatcag gaactcagaa cttctaaaga agaatttgaa aaaccatttc catatgaaga
     1501 tgagttaaat gagaaaacaa agcgtttagc acaattaaca atggaattaa aacttgatga
     1561 aagagaacca gatgttaatg atgattctga tatggaagaa cccgaagaag atattatcaa
     1621 ggaaagagaa tatgaaagat aaaataaaaa tattagaaag tcttcatttc ttatatcaaa
     1681 taaatcaaag tcacgagtat cgaaaagtat tcatgacttt ttaaatttta aggaggtact
     1741 atcatgtcgc atatcaaacc ccatgagtta aaagaaataa aaaagatcga tcttttcact
     1801 tatttaaaga actatcagcc agacagatta aaaaaaatat cacatgatac acactgtatt
     1861 cgtgatcatc aaagccttca tatttcaaat ggattatggc attggcaatc aacaggaatc
     1921 ggtggacgaa gtgctttaga cttcttaatc aaggtagata attattcatt tttagatgca
     1981 gctatgattt tattagagaa aacaaaagca aaggaaccta tctatacagc ctattcaccg
     2041 aaagaagaaa ataaaaaact tcaacttcct ttagcttcaa aaacaaatga acatgcttta
     2101 gattatttaa tgaggagagg aattgatcgt gaaatcattc aggattgtat tcaaaatcaa
     2161 ttgatttatg aaagcaaaag taggaatact tcaacaaata aaatttatac gaatgttaca
     2221 tttttaggtt atgatagatc gaatcatcaa cctaaatatg ccaatattag aaatgttgat
     2281 gtagatttta aaggtgatgt tgcaggtagt gacaagcact tttgtttctc aatagcatca
     2341 caacatttat caaatgaatt gcatattttt gaatcagcaa ttgatgcatt atcgtatgcg
     2401 accttattaa aaatgaatga aaaaaactat aaagatattc atttattatc gcttggtggt
     2461 gttgcaattc ctagaaaaaa tacaagcgaa caaataaaat ttcctgttgc acttaaacaa
     2521 tatcttgaag attttcccaa tataaataag gtaattcttc acctagacaa cgatcgtgct
     2581 ggacgtattg cttcacaaaa tattcaaaaa gaattatggt atatggaaac agaagatata
     2641 gtcccacgct atggaaaaga tgttaatgat gaattaaaat ttcgattagg aatgcctatt
     2701 tctttgaaaa ataaatacga tagacaaaag gaaagatagt aggaggtgga tcaatgataa
     2761 agaaaattga aaaggaaatc agaagattag aactgttgaa agaacaaaaa actgtaaaaa
     2821 gaaatcaact tgatgaggaa atatccgaag cgtctacgca attaaaagaa ctttactcat
     2881 taaagaatca gtatgaaaaa ctacaaaaca atacagccaa tttctttgaa aagaatgaga
     2941 acaccccaaa atctttttga ggtggtaatt cacatgccct tacagtcatg taggggtatt
     3001 gtgccataca ctataccagc aagcactgga tacggatagc cttttgacta tcctatccga
     3061 tcttgctagg gaaatcccta gtaccctttg tatctcaata agattaaatg aatactttca
     3121 atggatatta aaacttacga taattataaa ttagtattac taaaacattt tttaaatcta
     3181 ataaccttat catatcgttc aaaagaattt tgctgtataa tagatagtaa gaaaggatgg
     3241 tagtatgaat aaaacaattg agcaacttat tgagaatttg gatacaatac catttttatt
     3301 tgttgggtca gggttatcaa ggagatatta taatttacct gattggatag gattattaaa
     3361 agtaatggtt gataaattaa ataaagattc atttgcattt cgttcttatg aagatcgagc
     3421 atcttttgaa agtaatccat atggtattaa ccctaaaata gcttcattga tagaagaaga
     3481 ttttaataag gaatggtttc agaaccctgc aataagaaac ttagacgaaa tatatcttga
     3541 aaaagtagag aagggatgtt ctccttttaa agctgaaatg agttattatt taaaacaaaa
     3601 gtcagtttta cattcaaatt taaaagatga agttacttta ctgaataata ttgcaaaaaa
     3661 atcaatcgct gggattatta cgacaaacta tgatttgttt tttgaaacat atttatctga
     3721 gtataaagtt tatatagggc aacaagcact cttgttttct caattaca
//
LOCUS       DAOMTA010000213         3739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_853_length_3739_cov_2.004072, whole genome shotgun sequence.
ACCESSION   DAOMTA010000213 DAOMTA010000000
VERSION     DAOMTA010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_853_length_3739_cov_2.004072"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PLK68_06930"
     CDS             complement(<1..1377)
                     /locus_tag="PLK68_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HRM90180.1"
                     /translation="MIKIMKRLLTGVLALATVFTALPTTAVHAAQTQYWTESAERVGI
                     VEKVMNDGSIGSTFNEGHMTVEGEDAYCIDINTNFKNGYKTRADASTRMSADQIADVA
                     LSIEYVKQYTDSHKGISSQHAYLLRQLVVWQRLSIHLGWGCDNVRAAYDEIPKVVQDE
                     VFAGAKTFVKENKGRYDCYGYIYSGEGQDIGQFYAKLAVGNGKIQKSSSNTGITESND
                     CYSIAVATYGVYADKSCKEQLATLTTDNSGSTETVELKAATYYVKETKAPKGFQLDKN
                     VYTLTVKAGETATLKVSDTPKVTDTLIELFKIDMETSKADPQGNASLEGAEFVWKYYD
                     GYYTKDNLPAEPTRTWTTKTKAEKGSDNEIHYITRLADSYKISGDSFYTQNGTVCLPL
                     GTITVEEKSAPNGYLLEGAYMQAAGSSEQIKGLYVAQITEDGELAVLSGSNQYSVSDK
                     VIRGGVKIQ"
     gene            complement(1883..2206)
                     /locus_tag="PLK68_06935"
                     /pseudo
     CDS             complement(1883..2206)
                     /locus_tag="PLK68_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2470..2658)
                     /locus_tag="PLK68_06940"
     CDS             complement(2470..2658)
                     /locus_tag="PLK68_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM90181.1"
                     /translation="MINRDLSLIFLIAVPVLEAGLYFIVSRSHPYFEKVFHIYDELNS
                     VVQENLAGIRVVKSFICP"
     gene            complement(2670..2996)
                     /locus_tag="PLK68_06945"
     CDS             complement(2670..2996)
                     /locus_tag="PLK68_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM90182.1"
                     /translation="MSIHEIFFLTCKYSIFGKWGVVLIFCVTLGVICGMLSGSFCAIA
                     SAGFAQNLRQNMYYKIHEYSFANIDKFSTSSLVTRLATDVTNVQNAHMMTIRVAVRAP
                     SCWHFH"
     gene            2985..3593
                     /locus_tag="PLK68_06950"
     CDS             2985..3593
                     /locus_tag="PLK68_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM90183.1"
                     /translation="MNTQQLECFVRIADKLNFTKVTEELYITAPAVAHHIINLEELNT
                     SLFIRTSKMVKSTEPGSLFYSEAKDILQKIYMVEKKVKKLASQKLSILRIGCSSQVEL
                     EILEIPLIKLKEEFPTVYPQIIIQDYFILKSLFDNKQLDILISTKEMIAEAHDYIFKK
                     IKNISSYAIIAENSPFAKEKEICFIDLYDTPLITLHPRFIPF"
BASE COUNT         1186 a          771 c          588 g         1194 t
ORIGIN      
        1 ctggattttc acaccgccac ggataacctt gtcagataca gaatactggt tgctgccgct
       61 taatactgca agctcgccat cttctgtaat ctgtgccaca tataagcctt ttatctgttc
      121 tgaacttcca gcagcctgca tatatgcgcc ctccaataag taaccgtttg gagcagactt
      181 ttcttctacc gtgatagttc ccagcggcag gcagactgta ccgttttgtg tatagaaact
      241 atcgccagat attttatagg aatcagctaa acgggtgata tagtggattt cattgtcgct
      301 gcctttttcc gctttggtct ttgtcgtcca tgtgcgtgtc ggctctgctg gaagattatc
      361 ttttgtataa tatccgtcgt aatacttcca gacaaactct gcaccctcca aactcgcatt
      421 accctgtggg tctgctttgg aagtttccat atcaatcttg aaaagctcaa tcaaggtatc
      481 tgtgacttta ggtgtatcgc tcactttcaa ggtagctgtt tcaccagctt tgaccgtaag
      541 agtatacacg ttcttatcta actggaatcc tttgggagct ttggtttctt tcacatagta
      601 tgtcgcagct tttagttcca cagtttcagt actgccactg ttatctgtcg taagtgtggc
      661 aagctgttct ttgcagcttt tatcagcgta gacaccataa gtcgcaacag cgattgaata
      721 acagtcgtta gattctgtaa tccccgtatt actggaagac ttctgaatct tgccatttcc
      781 cacagccagt tttgcataga actgtcctat gtcctgtccc tcgccagagt agatataacc
      841 ataacagtca tagcgtcctt tgttttcttt gacaaaggtt tttgcacctg cgaacacttc
      901 atcttgtacc actttgggaa tttcatcata ggcagctcgc acgttatcgc agccccagcc
      961 taaatgaatg ctcaatctct gccagaccac taactgtctt aacaggtagg catgctgact
     1021 gcttatccct ttatgactgt ccgtgtactg cttgacgtat tcaatggata aggcaacatc
     1081 agctatctgg tcggcactca tgcgtgtgct tgcgtcagct ctggtcttat agccattttt
     1141 gaaattcgtg tttatatcta tgcagtaagc gtcctcgccc tcgacggtca tatgcccctc
     1201 gttgaatgtt gagccgatag aaccatcatt cattaccttt tcaacgattc caacacgttc
     1261 cgcagattcc gtccaatatt gcgtctgtgc tgcatgaacc gcagtagtgg gtaatgcagt
     1321 aaagacggtt gctagggcaa ggacacccgt aagcaatcgt ttcataatct ttatcataat
     1381 tttttgattc tcctttcttt tgaaatataa aaaggacgct catttctgaa cgtccaaagg
     1441 gtacgagtat cttgatttct agtgacaaat gatttatatt taaaaaagta aaaattgcaa
     1501 aaaactaagc gcatttgcta acagcttgct aacactctac ggtgtatggc agtacaccta
     1561 cgggtatcag cgtctagcaa taacaaaggc tttgtcttta ttttttgcca ttaagcgccc
     1621 cataaaggat aatcgaacca ccagctacca aagttcacag ggaactccta aggtttaggt
     1681 gcaggttcga ttcctgcagg ggacgccaat tataatttat aaaaatatat tattgaaatc
     1741 aggtggatta tagacctttt aaatccacct tttttatatg aatatttaac tttttataat
     1801 ctatcagaac acatacttac tatttctttc aaagatacct atacaaaaat aggattttca
     1861 taagcacttt tgacataaca tattactcta gttcaaaagc atcagtatag aaactataat
     1921 gaacatcatg ctttgaaatg agttcatcat gactccccca ttcaataatt ttcccaaaat
     1981 tcaaaacaat aatagtttca gaattacaaa ccgttgataa tcaatgagca attacatagg
     2041 ttgttctacc tctcattaaa ttatccattc cttgctgaac aagcacttct gtcctaatat
     2101 caatcgaaga agttgcttca tctaagatca taactggtaa atttgcaaca gccgcacaag
     2161 cgatagaaag caattgcctt tgtcttcgag ataaacttgc cccattttgc atctcataga
     2221 taggttccct tccatttttt aaagaacttt cttcatctga aacttcaatg attcgttcac
     2281 atgatgctct tgccatattg atcattacca atacttgtaa taacatcatc agacaattca
     2341 aaatattcat tgcataggca atcatacttg ataagtcccc tgtggtcatc gttgatgcat
     2401 ttataagaat aataattttt gctccaaacc agcaaagaaa caacaaacag ccataaactt
     2461 caaactgcat tatgggcata taaatgattt aactacccga attcctgcca aattttcctg
     2521 tactacacta ttcaattcat catagatatg aaataccttt tcaaaatatg gatgtgaacg
     2581 actaacaata aaataaagac ctgcctctaa aactggaact gcaattaaaa atattaatga
     2641 taaatcccta tttatcatta atgaaacagc tagtgaaaat gccagcatga tggtgctcgc
     2701 actgccacac gaatagtcat catatgtgca ttttgaacgt tagttacatc agtcgctaat
     2761 cttgttacca aacttgaagt agaaaactta tcaatatttg caaaagaata ttcatgaatt
     2821 ttataataca tattctgtct tagattttga gcaaatccag ctgaagctat cgcacaaaaa
     2881 cttcctgata acatgccaca aataactcct aaagtaacac aaaagattaa gactacaccc
     2941 catttgccaa aaatactata tttacaagtc aggaagaata tttcatgaat actcaacaat
     3001 tagaatgttt tgtgcgaata gcagataaat tgaattttac aaaggttact gaagaattat
     3061 atattactgc tccagcagtt gcccatcata tcataaattt agaagaatta aatacgtcat
     3121 tatttatcag aacttccaaa atggtaaaat caactgagcc aggctcatta ttctatagtg
     3181 aagcaaaaga tattctccaa aaaatatata tggttgagaa aaaagttaaa aaactagcca
     3241 gtcaaaaact ttcgatatta cgaattggct gttctagtca agtagaactt gagatattag
     3301 aaataccatt aataaaatta aaagaagaat ttccaactgt ttatccccag attattattc
     3361 aggattattt tattctaaaa tctctatttg ataataaaca attagacatt ttaatcagta
     3421 caaaagagat gattgctgag gctcacgatt atatatttaa aaaaataaaa aacatttcca
     3481 gttatgcaat catagctgaa aattccccgt ttgctaaaga aaaagaaata tgttttatag
     3541 atttatatga tacaccactt attactttgc atccccgctt tatcccattt taatactgag
     3601 ataaattaca agaatttatt gctctccatg aaaaaatcat tttcatcttt tatgtaaaaa
     3661 tgaccaggct gctattcttt tcgctaaatg taattacggt gtagctattt taccagaaat
     3721 atatattcca acacatatt
//
LOCUS       DAOMTA010000214         3727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_856_length_3727_cov_2.753540, whole genome shotgun sequence.
ACCESSION   DAOMTA010000214 DAOMTA010000000
VERSION     DAOMTA010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_3727_cov_2.753540"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1538
                     /locus_tag="PLK68_06955"
     CDS             213..1538
                     /locus_tag="PLK68_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM90184.1"
                     /translation="MKKYSVCIVGGGSTFTLGFLKSFCRMKEQFPLKKLVLFDIDAER
                     QEPIGEFGKILFKEQFPELDFSYTTDIKEAYEGMDFVFMQMRAGGLPMRAKDEHIPLS
                     MGLIGQETCGAVGMAYGLRSCVDMIKAVHDIRNYAPEAWILNYSNPAAIVAEALRREF
                     PDDKKILNICDQPENVVRSCSRLLGCDWENLDPVYFGLNHYGWFTNIYDIKTGEDLLP
                     KLRELIKKNGFLPQDAEQRDQSWLDTYGMVETLMNDFPDFLPNTYDQYYLYPDYKAAH
                     LDPNFTRADEVMAGREKRVFDECREVIAEGVLGDKFDDISDAHAEMMINVAEAIAYNK
                     NTRHILIVENNGAIANMQDDAMVEVVCELGINGPRPMRVGNIPQFYLGLLVNQVSCEK
                     LIVDAYFENSYQKALMAFTLNRLINDGKKARKVLDALIEANKGMWPELK"
     gene            1559..3085
                     /locus_tag="PLK68_06960"
     CDS             1559..3085
                     /locus_tag="PLK68_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90185.1"
                     /translation="MKKNKVMDFFSALGRSLMMPIAALAACGIILGVTAALLKTQVVE
                     AVPFLQQPVVFYILNTLKTVSNVVFTLIPVLFAISISFGMAKEDKEVAAFAGFIGYYT
                     FLVSASCMINSGFMNFDSLQISTILGVETLDMGAVAGILTGVTVATLHNKYHKVVFPV
                     AIAFYGGKRFVAIVVILAMALLGQVAPFIWAPVSAGINGLGTLISESGLLGVFSFGFL
                     ERLLIPTGLHHVLNGIFRTTAIGGVYQGVEGCLNIFLQFFDSVDISVMREYTQFLGQG
                     KMLFMIFGLPAAAYAIYKTSPGEKKNKVKALMIAGVAASIVSGITEPLEFAFMFIAPP
                     LFLFHAVMGGISFMLMSLLQVAVGNTGGGIIDMFIWGVFQPGSNWYWIIIIGPIYAII
                     YYNVFKWYFNRKHLSIEVAEDDGDKDTNDTASISDKQQVLATKIIEGLGGFDNIITVN
                     NCISRLRVDVKDMSLVNEEELKKTGSMGIVKPSETHIQVIYGPKVEQVANSVREVLKY
                     "
     gene            complement(3103..>3727)
                     /locus_tag="PLK68_06965"
     CDS             complement(3103..>3727)
                     /locus_tag="PLK68_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90186.1"
                     /translation="YMSNASIMRFCNKLGLSGFNELKYELKQSMHQEIDYQMIIEKPL
                     SRFLDNLKNLNYEIIEEVVDLLCSPQPIYVVGRSLSLVAADYFQTILSSIDINCILIN
                     DLHLSKSVFNNLKAPATIFIVSANNAGKIYDEVVTIAKTHDCKIILLTSNAKGSLVDS
                     CDYVLSSNDENLTYHGVDINSRLGIFTIMQIIIELTAQKLALEKATD"
BASE COUNT         1299 a          462 c          696 g         1270 t
ORIGIN      
        1 attaaatttt tacttatgaa taaagttatt ttttgaaata tattataatt atttgaaaaa
       61 aataacattt aatgtcacaa ataacaagtt agggctttca ttcccaaaca aggccaaagt
      121 attgtttaaa taaatgtgtt atgagatagt aaatatgtac ttgataggcc gtcaaagtaa
      181 agcagaaaaa gtagaaagag agataaacaa aaatgaaaaa atatagtgtt tgtattgttg
      241 gaggaggaag tacctttaca ttaggttttt taaaatcatt ctgtcgtatg aaagaacagt
      301 tcccattaaa aaaattagtt ttatttgata ttgatgcaga gcgccaagaa ccaattggtg
      361 aatttggaaa aattttattt aaagaacaat ttcctgaatt agatttctca tatacaactg
      421 atattaaaga agcatatgaa ggaatggatt ttgtgtttat gcaaatgcgt gcagggggat
      481 taccaatgcg tgctaaagat gaacatattc ctttaagcat gggattaatt ggacaagaaa
      541 catgtggagc agtaggaatg gcttatggac ttagatcatg tgttgatatg atcaaagcag
      601 ttcatgatat tcgtaattat gcacctgaag cttggatctt aaattattca aatccagctg
      661 ctattgtagc tgaagcatta agaagagaat ttcctgatga taagaaaatt ttaaatattt
      721 gtgatcaacc agaaaatgtt gttcgttcat gcagtcgttt attaggttgt gattgggaaa
      781 atttagatcc tgtatatttt ggtttaaatc attatggctg gtttactaat atatacgata
      841 ttaaaacagg ggaagattta ttaccaaaat tgagagaatt aattaagaaa aatggcttct
      901 taccacaaga tgcggaacaa agagatcaat cttggttgga tacttatggt atggttgaaa
      961 ctttaatgaa tgattttcca gattttttac caaacacata tgatcaatat tatttatatc
     1021 cagattataa agcagcacat ttagatccta atttcactag agctgacgaa gtaatggcag
     1081 gacgtgaaaa acgagttttt gatgaatgtc gtgaagttat agctgagggt gttttaggtg
     1141 ataaattcga tgatattagt gatgcccatg cagaaatgat gatcaatgta gctgaagcaa
     1201 tcgcatataa taaaaatact cgtcatattt taattgttga aaacaatgga gcaatcgcta
     1261 atatgcagga tgatgctatg gttgaagtcg tttgtgaatt aggaattaat ggaccaagac
     1321 caatgcgtgt aggtaatatt ccacaatttt atttaggatt gttagtaaac caagtttctt
     1381 gtgaaaaatt aattgttgat gcttattttg aaaattcata tcaaaaagca ttaatggcat
     1441 ttactttaaa tcgtttaatc aatgatggta aaaaagctcg taaagtatta gatgctttaa
     1501 ttgaggctaa taaaggaatg tggccagaat taaaataaaa taataaaagg ggattagtat
     1561 gaaaaaaaat aaagtaatgg atttcttctc ggctttagga agaagtttaa tgatgccaat
     1621 tgcggcttta gcagcatgtg gaattattct gggagttact gcagctttac ttaaaacaca
     1681 agtagtagag gcagtaccat ttctacaaca gccagttgta ttttatatct taaatacact
     1741 aaaaacagta tcaaatgttg tttttacctt aataccagta ttatttgcta tttctatttc
     1801 atttgggatg gctaaagaag ataaagaagt tgcagcattt gctggattta ttggctatta
     1861 tactttctta gtaagtgcaa gttgtatgat caattctggt tttatgaatt ttgattcatt
     1921 acaaatttca actattttag gcgtagaaac tttagatatg ggagcagtag ctggtatttt
     1981 aacgggggtg accgtagcga ctttgcataa taaatatcat aaagtagtat tcccagtagc
     2041 aattgctttt tatggtggta aacgttttgt tgcaattgtc gttatcttag caatggcgtt
     2101 attaggacaa gtagcaccat ttatttgggc acctgtatca gctggaatta atggtctggg
     2161 tacattgatt tctgaatcag gtttattagg ggtattctca tttggatttt tagaaagatt
     2221 attaattcca acaggattac atcatgtttt aaatggtatt tttagaacta ctgcaatcgg
     2281 tggggtatat caaggggtag aaggatgttt aaatattttc ttgcaattct ttgatagtgt
     2341 tgatattagt gtaatgcgtg aatatacaca gtttttggga caaggaaaaa tgttgtttat
     2401 gatctttgga ttacctgcag cagcctatgc tatttataag acaagtccag gtgaaaaaaa
     2461 gaataaagtt aaagcattaa tgattgcagg agttgcagca agtattgttt ctggaattac
     2521 tgaaccatta gaatttgcat ttatgtttat tgcaccgcca ttattcttat tccatgcggt
     2581 tatgggtggt atctccttta tgttaatgtc attattacaa gtagcagttg gaaatactgg
     2641 tggcggtatt attgatatgt ttatctgggg tgtcttccaa ccaggatcta attggtattg
     2701 gataattatt attggtccaa tctatgcaat tatctattat aatgtattta aatggtattt
     2761 taaccgcaaa catttatcaa ttgaagttgc ggaagatgat ggagacaagg atactaatga
     2821 tactgcgagt attagtgata aacaacaagt gctagcaaca aaaataattg aaggcttagg
     2881 aggatttgat aatattatta ctgttaacaa ttgtatttct cgtctaagag ttgatgtaaa
     2941 agatatgtca ttagtaaatg aagaagaact taagaagaca ggaagtatgg gaattgttaa
     3001 accatcagaa actcatattc aagtaattta tggtcctaaa gtagaacaag tagctaattc
     3061 agttagagaa gttttaaaat actaattaaa aaggcaacga atttaatcag ttgccttttc
     3121 taaagctaat ttttgagcag ttagttcaat aattatttgc ataattgtaa agatacctag
     3181 ccttgaattt atgtctacgc catgataagt tagattttca tcattactac ttaaaacata
     3241 atcacaagaa tctactaatg aacctttagc gttagatgta agtaaaataa ttttacagtc
     3301 atgggtctta gcaatcgtaa ctacttcatc atatatcttt ccagcattgt ttgctgatac
     3361 aataaaaatt gtagcaggag cctttaaatt attaaagaca ctttttgata gatgaagatc
     3421 attgattaaa atacaattaa tatcaataga tgataaaata gtttgaaaat aatcagcagc
     3481 cactaagctt aaactcctac ctacgacata gatcggttga ggactacaca ataaatcaac
     3541 aacttcttca ataatttcat aatttaagtt tttaagatta tcaagaaaac gggataaggg
     3601 cttttcaatt atcatttgat aatcaatttc ttgatgcata ctttgcttta attcatattt
     3661 tagttcatta aaaccagata agcctagctt attacaaaag cgcatgattg aagcattaga
     3721 catatac
//
LOCUS       DAOMTA010000215         3704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_863_length_3704_cov_2.004385, whole genome shotgun sequence.
ACCESSION   DAOMTA010000215 DAOMTA010000000
VERSION     DAOMTA010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_3704_cov_2.004385"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..2153)
                     /locus_tag="PLK68_06970"
     CDS             complement(51..2153)
                     /locus_tag="PLK68_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM90187.1"
                     /translation="MNFLIFVALVFVQAWSNLVFMAIIVTNSVIGIWQEVKAKKLVDQ
                     LSILTKPTIMVVRNSRHVIVDINEVVLDDVLVLESGNQVCNDATVIHGMIEANESLLT
                     GESDPIHKNIGGHLLSGSSVVSGKCYARVKNVGDSNYATTIVKEAQKVKEKNSELMDS
                     MNKVTKVTTFMIIPLGIILFVEAMYLRQDTLFNAVVSSAAGLLGMLPKGLVLLTSVSL
                     ANGVTRLARRKVLIQDLYSLETLSHVDVLCLDKTGTITDGKMKVEKTYTLETTQNFKL
                     DEVMGSYMRASDDNNATFQAMNSYFGENNLYEYVKKIPFSSLRKWSAIEFKGIGTIVV
                     GAAEKIISGELPEDIHELMLQGMRAIAIGYTEKTVDDKEELPRLQPLMAIILSDTIRN
                     NTKETLEYFHQEGIDAKIISGDNVNTVMAIAKKAGVLNYERCIDMSTINDDEIQEVVR
                     NYTIFGRVTPSQKKMIVEALKNDGHHVAMTGDGVNDLLALKEADCSIAIADGSDASKQ
                     ISQVVLLNSDFTCLPDVLLEGRKVVNNVTRVAGVFFIKTIYTILLSLFCVISNTAFPF
                     IPLQITLIDLLIEAMPSFMTIFEADTRKITGRFLPKVFSKAAGNALSIVILFIAIMIF
                     GPMWKINDLELVTLMYLVLGTISMAAVIRSCYPFTMLRIIICTMMAGGFYGAVLLFSG
                     LLHLAPITLNLVFIALSV"
     gene            complement(2379..2948)
                     /locus_tag="PLK68_06975"
     CDS             complement(2379..2948)
                     /locus_tag="PLK68_06975"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM90188.1"
                     /translation="MKEKVRKFSKLAYWLILGMILMYIVGMQFYAQEITDFIGYRFYA
                     VMTDSMEPRIPTYSMVLTKTIDENESIAPGEIITFKVQRGEQEIVLTHHFNKTQEENG
                     QLYYRTNAEGRDELDLYYTKRSDIIGKYVWHIPLLGKFIMFIQSKFSWILYCEFLLIW
                     LINKTIKAHWEEKGELDDEVKIPRKLDNA"
     gene            complement(3002..3514)
                     /locus_tag="PLK68_06980"
     CDS             complement(3002..3514)
                     /locus_tag="PLK68_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90189.1"
                     /translation="MKKSTLLTMASVGAVALTSAMTFAAWDNLSDTTTSNEVTFKQIN
                     VEKAADIVLTEPVASDLTSSYVPSSSGEVTFNITGIDAADLSGKELKLEPVVKANDTA
                     ISTGYTLEIYDGTEESAEKVAGNVDKSLTGTNTYKVVVTATDDKAGTDALANKKVTVE
                     LKATLQKTTV"
BASE COUNT         1215 a          733 c          448 g         1308 t
ORIGIN      
        1 atttatggtc tttcagattt tatagggtta ttgataattt acaatgcaaa ttaaacagat
       61 aaagcaataa aaactaaatt taatgttatc ggtgctaaat gtaataaacc actaaataat
      121 aatactgcac cataaaaacc tccagccatc atcgtacaga taataattct taacattgta
      181 aaaggataac aagagcgtat taccgctgcc atagaaatag ttcctaaaac taaatacatt
      241 aatgttacta gttctaaatc atttattttc cacattggtc caaaaatcat gatagcaatg
      301 aataaaatca caatacttag tgcattacca gctgctttac taaatacttt aggcaaaaaa
      361 cgtccagtaa ttttacgagt atcagcttca aaaatcgtca taaacgatgg cattgcttca
      421 atcaataaat caattaatgt aatttgtaac ggaataaatg ggaaggcagt attactgatt
      481 acacaaaaca acgataataa aatcgtataa attgttttga taaagaatac acccgctaca
      541 cgcgtaacat tattaacaac tttacgtcct tcgagaagaa catctggtaa acatgtaaaa
      601 tctgaattta ataaaactac ttgtgagatt tgttttgaag catcacttcc atcagcaatt
      661 gcaattgaac aatcagcttc ttttaacgct aacaggtcat taactccatc acccgtcata
      721 gctacatgat gaccatcatt ttttaaagct tcgacaatca ttttcttttg actaggagtt
      781 acccgaccaa aaatagtgta attacgaact acctcttgaa tttcatcatc atttatcgtt
      841 gacatatcaa tgcaacgttc ataatttaat acccctgctt ttttagcaat tgccataact
      901 gtatttacat tatcaccaga aataattttc gcatctattc cttcttgatg aaaatattct
      961 aatgtttctt tagtattatt tctaattgta tctgataaaa taatcgccat tagtggttgt
     1021 aatcgtggca actcttcttt atcatcaaca gtcttttcag tataaccaat ggcaatcgca
     1081 cgcattcctt gcaacattaa ttcatgaata tcttcaggca gttcaccaga aataattttt
     1141 tctgctgctc ctacgacgat ggttcctata cctttaaatt caatcgcact ccacttacgt
     1201 aatgaagaaa atgggatctt tttaacatat tcataaagat tattttctcc aaaatagcta
     1261 ttcatcgctt gaaaagttgc attattatca tcactggcac gcatatatga gcccattact
     1321 tcatcgagtt taaaattctg agttgtttct aacgtatatg ttttttcgac cttcattttt
     1381 ccatcagtga ttgttcctgt tttatctaaa cacaaaacat caacatgcga taatgtctct
     1441 aatgaatata aatcttgaat caacacttta cgtcttgcta aacgagtcac tccatttgct
     1501 aacgacacac tagttaacaa gactaaccct ttaggcaaca ttcctaataa tcctgctgcc
     1561 gaagagacaa ctgcattaaa taacgtatct tgtcgtaaat acattgcttc aacaaacaaa
     1621 ataattccca atgggattat cataaaagta gtaacttttg taactttatt cattgaatcc
     1681 attaattcag aattcttctc tttaactttt tgtgcctctt taacaatagt tgttgcataa
     1741 ttactatcac caacattttt aacccttgca taacattttc cagaaactac actactacct
     1801 gacagcaggt gcccacctat atttttatga atcggatctg attccccagt taatagtgac
     1861 tcattggcct caatcattcc atgaatcaca gttgcatcat tacatacttg atttccactt
     1921 tcaagaacta aaacatcatc tagaacaact tcattaatat cgacaattac atgtctgctg
     1981 ttacgaacaa ccataattgt tggcttagtt aaaattgata attgatctac tagttttttt
     2041 gcctttactt cttgccaaat tccaataact gagttggtaa caataattgc cataaaaact
     2101 aaatttgacc aagcctgaac aaatactaaa gctacaaata ttaaaaaatt caaaagatta
     2161 aataatgtac aggtttacta actccgttaa cttgcccctt agcaattctt tcttggactt
     2221 cactatcatt taaacccact ttataataca taggtaattc cttttccatc tattccacct
     2281 ccggatttct catcattata ttacactttg ttgagcaagc atatacactt tactaattct
     2341 taacattccc ttaactaaaa taaaacaaaa aaggataact aagcgttatc caattttcgc
     2401 ggtattttaa cctcatcatc aagctctccc ttttcttccc agtgagcttt tattgtttta
     2461 ttgattagcc aaatcagtaa aaattcacaa tatagaatcc aactgaattt gctttgaata
     2521 aacataataa actttcctaa taatggtata tgccaaacat atttaccaat gatatcacta
     2581 cgttttgtat aatatagatc taattcatct cgaccctcgg catttgttcg ataatataat
     2641 tgtccatttt cttcttgtgt tttattaaaa tgatgtgtca acactatttc ttgctctccc
     2701 cgctgaactt taaatgtaat tatttctcca ggggcaatag attcattttc atcaattgtt
     2761 ttagtaagta ccattgaata cgttggaatt ctaggttcca tgctatccgt catgactgca
     2821 taaaagcgat atccaataaa atcagttatc tcttgagcat aaaattgcat cccaacaata
     2881 tacatcaata tcattcccaa tatcagccaa tatgccagct tgctaaattt tctcaccttt
     2941 tctttcatga ctttcacatc ctatattata tacttggaca aaaatgccat tattgatagc
     3001 attaaacagt agtcttttgt aatgttgctt ttagttcaac agttactttt ttatttgcta
     3061 acgcatcagt tccagcttta tcatctgttg cagtaactac tactttgtaa gtattagttc
     3121 cagtaagact tttatcaaca ttaccggcga ctttttctgc cgattcctct gttccatcat
     3181 aaatttctaa agtatagcca gttgaaattg ctgtatcatt agctttaact actggttcta
     3241 attttagttc cttaccactt aaatcagctg catctatacc tgttatatta aatgttactt
     3301 ccccacttga actaggtacg taagaactag ttaaatcact agcaactggc tcggtaagaa
     3361 caatatcagc tgccttttcc acattaattt gtttaaatgt cacttcattg cttgtagtag
     3421 tatcagataa attatcccat gccgcaaagg tcattgcaga agttaaagct acggcaccaa
     3481 ctgatgccat tgttaataat gtactctttt tcataataat tttctccttt agtatttttt
     3541 atattatatg tgtcggaatt tgtcattcct gtttctatac ctatattcta atactaaatt
     3601 caaaaagcag ttaatactta tcttggtagg tctagttata ctttttgtgt atatctacat
     3661 atccgcataa ttatcgttaa taatttctaa aaatatttta gcag
//
LOCUS       DAOMTA010000216         3695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_869_length_3695_cov_2.446978, whole genome shotgun sequence.
ACCESSION   DAOMTA010000216 DAOMTA010000000
VERSION     DAOMTA010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_869_length_3695_cov_2.446978"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLK68_06985"
     CDS             <1..353
                     /locus_tag="PLK68_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90190.1"
                     /translation="IYQNSQMGVETIEQLEKIVEDKDFKRYLKEKYEGYCKIHKDAKE
                     KLNSHGYDERGIGSFEKIRTYLMINMQTLTDKSTSHIAEMMMIGSTMGIINAIRNIAD
                     YNHAKKILLSSWKH"
     gene            complement(510..1151)
                     /locus_tag="PLK68_06990"
     CDS             complement(510..1151)
                     /locus_tag="PLK68_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90191.1"
                     /translation="MFKRVIVLVFGVVVVACSSALTLKAAIGVGAWDALAQTGSLVTG
                     IEVGTIGMFCNFLCIFVQVAILRRKFKPIQLLQIPVCILLGLIVNFMLYEVYSNFTIN
                     TYWMNVGLLILGYIVSAFAVGMVMVLDIVTFALEGACMAVSGVTGKKFHVLRQGVDVV
                     SILLVIIIVIITDVPLAVREGTIIGMFLFGPMMGIFMKLQKPVFKKFGLIDYN"
     gene            complement(1263..2048)
                     /locus_tag="PLK68_06995"
     CDS             complement(1263..2048)
                     /locus_tag="PLK68_06995"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HRM90192.1"
                     /translation="MSRIEAVIFDWAGTTVDYGCFAPVQAFKDAFNNYGLEPTNDQIR
                     EPMGMLKIDHIKTMLEMPVLNEAFKARYDREFNEQDIHKIYDLFEASLMTGITKHTDV
                     KPYVLETVAKLRELGIKIGSTTGYTDMMMEPVLKSAKEQGYQPDCWYSPDATNHFGRP
                     YPYMIFKNMIELHISSVKNVIKVGDTISDIQEGVNAGVIAVGVIEGSSTLGLNEEEFN
                     ALTPKERNRAIERVKEAYLDAGADYVINNLSELIALIQEVELF"
     gene            complement(2048..3142)
                     /locus_tag="PLK68_07000"
     CDS             complement(2048..3142)
                     /locus_tag="PLK68_07000"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HRM90193.1"
                     /translation="MKDYKLLTPGPLTTTATVKQEMLFDHCTWDDDYKKITLKIRDQL
                     LELAHVRAEQYTVVLMQGSGTFGVESVITSVIGENEKLLIVANGAYGKRMKDICEHAR
                     INHEIIEFAENENPSAVAVAEKLDEEKEITHVAIVHSETTSGILNDIESVAKVVKERN
                     KVFIVDAMSSFGGVDIEVGKLGIDFIISSANKCIQGVPGFSFVIANKNLLLASRGKAR
                     SLSLDLYDQWETMDKDGKWRFTSPTHVVLAFSKALDEMLEEGGIAARSKRYYDNNRLL
                     IEKMAEMGMKSYVDLAYQGPIITTFYYPEGKEFAFSEMYDYIKARGYAIYPGKLTTAQ
                     TFRIGNIGEIYEADILNLCSIIKDFLAEVA"
     gene            complement(3152..>3695)
                     /locus_tag="PLK68_07005"
     CDS             complement(3152..>3695)
                     /locus_tag="PLK68_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM90194.1"
                     /translation="DLAKPEYKDMISVTDIASSSTAWLLIQALVSEYGEDGAKEVLKG
                     IYDNAGAHIEDSGSGPIKKVRAGEVAIGFGLRHQAVRDKADGLPIDFVDPTEGNFSLT
                     ESVAVVNKDGSEHQKLAMKMAQCIIESGRKELLETYPNPLYQGETSDSANKSAYPKTF
                     SEKLTAELLEKHQKLSEECK"
BASE COUNT         1205 a          761 c          462 g         1267 t
ORIGIN      
        1 ttatttatca aaactcgcaa atgggagttg aaacgattga gcaattagaa aaaatcgttg
       61 aagataaaga ctttaaaaga tatttaaaag aaaaatatga aggctattgt aaaatccata
      121 aagatgctaa agaaaagtta aatagtcatg gatatgacga aagaggaatt ggttcttttg
      181 aaaaaattcg tacttactta atgatcaata tgcaaacact gaccgataaa tcaactagtc
      241 atattgcaga aatgatgatg attggaagta cgatgggaat catcaatgca attcgtaata
      301 tagctgatta caatcatgct aaaaagatat tattaagctc atggaaacat taaaagcttt
      361 cgaagaaaaa tcatatggag atttgcaaaa atttatttaa attgctgtta atgcagtgct
      421 ctcctaagtt caactttgtg agggcactac atatgaggta tattatttct aattaaaaac
      481 agtattacaa ataatactgt ttactatcac taattataat caattaaacc aaatttctta
      541 aacactggtt tttgcaattt cataaaaatc cccatcattg gaccaaataa aaacattcca
      601 ataatagttc cctctcgtac tgctaaaggc acatcagtaa taattacaat tataattact
      661 aataaaattg aaactacgtc aaccccttgc cgtaaaacat ggaatttctt tccagtaact
      721 ccgctaacag ccatacaagc tccttccaaa gcaaatgtaa caatatctag aaccattacc
      781 attcctactg caaaagcact gacgatatat cctaaaatta ataatccaac attcatccaa
      841 tatgtattaa tcgtaaaatt actgtatact tcatacaaca tgaagttaac aattagccct
      901 aataagatgc aaacaggaat ttgtaatagc tggatcggct taaattttct tctaagtatt
      961 gccacttgaa caaagataca taaaaaatta caaaacatac caattgttcc tacctctatc
     1021 cctgtaacta aactccctgt ctgtgccaaa gcatcccatg cacctactcc aatggccgct
     1081 ttcaaggtta gagctgaact acatgcaacg accacaacgc caaacaccaa aactattacc
     1141 cgcttaaaca ttttaattag tacctcgctt tttttgtata ggcattatta tagcggtttc
     1201 aataatctag tcaacctatt ttttaagcgt tttcaaaaat agaaaaaggg taatctaccc
     1261 ttttaaaata actctacttc ttgtatgagt gcaattagct cgcttaagtt attaattaca
     1321 taatcagcac cagcatctaa atatgcttct ttaacccgtt caattgcacg attccgttct
     1381 ttaggtgtta aagcattaaa ttcttcttcg ttcaatccca aagttgaact tccctcaatg
     1441 acaccaactg cgattacccc tgcattaaca ccttcttgaa tatcagaaat tgtatcacca
     1501 actttgatta cattttttac actgcttata tgcagttcaa tcatattttt aaaaatcata
     1561 taaggataag ggcgtccaaa gtgattagta gcatcaggtg agtaccagca atcaggttga
     1621 tacccctgct ccttggcact ttttaatact ggctccatca tcatatctgt atatccagta
     1681 gttgagccaa tcttaattcc caattctctt agttttgcca cagtttctaa aacatacggc
     1741 ttaacatctg tatgttttgt aatacctgtc attaatgatg cctcaaataa atcataaatt
     1801 ttatgtatat cctgttcatt aaattcacga tcatatctag ccttaaatgc ttcattcaat
     1861 actggcattt ctaacattgt tttaatatga tcaatcttca acatccccat tggttctcta
     1921 atttggtcat tagttggctc taatccataa ttattaaaag catctttaaa tgcttgaaca
     1981 ggagcaaaac aaccataatc aactgtagtt cctgcccaat cgaaaattac tgcttcaatt
     2041 ctactcatta tgctacctcc gctaaaaaat ctttaataat tgaacataaa tttaatatat
     2101 ctgcttcata aatttcacca atattaccaa ttctgaatgt ttgagcagta gttaactttc
     2161 ctggataaat tgcataacca cgtgctttta tataatcata catctcacta aaagcaaatt
     2221 cttttccttc aggataatag aaagtagtaa taattggtcc ttgataagct aaatcaacat
     2281 agcttttcat tcccatttca gccatttttt caattagcaa acgattatta tcataataac
     2341 gtttacttcg agcagctatt ccaccttctt ccaacatctc atctaacgct tttgaaaatg
     2401 ccagtacgac atgggttggt gaagtaaaac gccattttcc atctttatcc atcgtttccc
     2461 attgatcata taaatccaat gataaacttc ttgctttacc tcgactagct agtaataaat
     2521 ttttattagc aatcacaaat gaaaaaccag gaactccttg aatacacttg tttgcgctac
     2581 ttataataaa atcaatccct aattttccta cctcaatatc aacaccacca aaactactca
     2641 tagcatcaac gataaatact ttatttcttt ctttaactac ttttgctaca ctctcaatat
     2701 catttaagat cccagatgtt gtctcactat gaacgatagc aacatgagtt atttcttttt
     2761 cttcatctaa tttttcagca acagctactg cactaggatt ttcattttca gcaaactcaa
     2821 ttatctcatg attaattctt gcgtgctcac atatatcttt cattcttttt ccataagcac
     2881 cattagcgac aatcaacagt ttttcatttt cgccaattac acttgtaatt actgactcta
     2941 ctccaaaagt cccgcttcct tgcatcagta ctacagtata ttgctcagct cttacatgag
     3001 ctaattctaa taattggtcc ctaattttta aagtaatttt tttatagtca tcatcccatg
     3061 tacaatgatc aaataacatt tcttgcttta ctgttgctgt tgttgttaac ggccctggtg
     3121 ttaataattt ataatctttc attttcttct cctacttaca ttcttctgat aatttttgat
     3181 gtttttctaa taattcggca gtcaattttt cactaaaagt ttttggatat gctgacttat
     3241 ttgcactatc actagtttct ccttggtaaa ggggattagg gtaggtttct aacagctctt
     3301 tacgtccact ttcaataata cattgtgcca tcttcattgc taatttttga tgttcactac
     3361 catctttatt tacgactgca actgattcag tcaaactaaa atttccctct gttgggtcaa
     3421 caaaatcgat tggaagtcca tcagctttat cacgtaccgc ctgatgacgt agaccaaagc
     3481 caatcgctac ttctccagct cgaacttttt taattgggcc tgatcctgaa tcttcgatat
     3541 gtgctccggc attatcataa attcctttta atacttcctt tgctccatct tcgccatatt
     3601 cacttactaa tgcctgaatt aataaccatg ctgttgatga agatgctata tccgtaactg
     3661 agatcatatc tttgtattct ggtttagcta aatct
//
LOCUS       DAOMTA010000217         3691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_872_length_3691_cov_2.308856, whole genome shotgun sequence.
ACCESSION   DAOMTA010000217 DAOMTA010000000
VERSION     DAOMTA010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_3691_cov_2.308856"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..>1656
                     /locus_tag="PLK68_07010"
     CDS             137..>1656
                     /locus_tag="PLK68_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM90195.1"
                     /translation="MDTIIIVIKDSDDCLRLKEFLSSDYHIVICSELENIIDNLNDNR
                     IVAIIMDLDDATEVNVAKYSSYKNIDNIQIPLIVGGNFTGNLLNLLNLGVSEVIVKPY
                     NEAIIKLRINNAIRRAEDNEYRIMADFDTLTKIYNRTAFYREAKKLITQNQDINYDLA
                     CFDIDRFKIINDIYGSSVGDELLIYIANTGVKRMKKLGGLIGRLSGDLFAIVVPHQAN
                     IEDLLLQQMNEDLGNFDLGIKVVVSFGYYNIDDLSLPVNNMCDRAMMAIKKVKDKYNT
                     SYATYDDELRDQVLEEQRIIDEMDRAFENDEFIPYYQPKFNMVTRTYIGYEALMRWQS
                     PTRGMVLPSTFIPVFEKNGFISKADRVIWRKVCQDMVIARQKGHVLLPVSVNVSRIEL
                     YDPELGNTILKLLAEYELSIELFQLEITETAYMQDSNQMIEAVVKLKELGFTILMDDF
                     GSGFSSLNILKELPFDIIKIDLAFLEHFDKNNKAEKILKSVIQMAKRLNMEVIAEGV"
     assembly_gap    1657..1666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1667..3102
                     /locus_tag="PLK68_07015"
     CDS             <1667..3102
                     /locus_tag="PLK68_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM90196.1"
                     /translation="TKRQEDFLVELGCNRAQGYRFAKPASASKIAYMVENGVIGVGDT
                     KDEDSAIVNIDDILTTVYQQGEVDWYRQALLELNAQVFEYDFKRDNMIVFDTPTKENG
                     SNLAKMEIPNYLYNVSIGRIVHSKDVKRYQTVFNGNQEFKVIYRRFMINHGSGYTWVK
                     DTGRIIYDEDNKPKICIGVSRVISDEKLNEQMMNVLSVMEQSTDFDSSINHILAEIGD
                     DFLLDRITLLLEEGNNYRSVYAWQDESIELEISDTFPIVRTELQEIVTYFKEHQIIVV
                     NKNEGNQFSKQINKNFFDNQVKTLVIISLNDDIGEFIGCIVFTMINDYRQWRDDELWA
                     LQELVKGINVYLNKEKIFNRLDSILLTYNRVMEKLNDGILMFTYESQPQLLFINEAYR
                     KILNIDDINVTNFLSTYYRSVDLDVQKQIHQAIEKCVETGEEQTLYHPLKTCEKQIIK
                     AKTIYNLSPVKENGKLVIISITTKQCT"
     gene            3152..>3691
                     /locus_tag="PLK68_07020"
     CDS             3152..>3691
                     /locus_tag="PLK68_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM90197.1"
                     /translation="MAEILIIEDEVKLRQELEVYLNNNGYLTKVIESFENTLEQMNQF
                     DGDLILLDINLPNVNGQFLCKEYRKNSSKPIIIVTSRNNEMDELMCINYGADDFVTKP
                     YNPLILLARIEAVLKRTQPTSDVLISYEKIKLDVSKSSIIDKNKVIELSKNELKIFYF
                     YHNNCSFIVINITIIWCREY"
BASE COUNT         1307 a          416 c          720 g         1238 t
ORIGIN      
        1 ggatatcggg cctatacagg ggtaatcaaa gaaaaactga tagattgaat tagaaaatgg
       61 ggatagctaa tagattgttt aattacaaca atgatgctag aatactacta atggttgatt
      121 tggggaggaa gatattatgg atacaattat catcgttata aaagatagtg atgattgttt
      181 aagattaaag gagtttttaa gtagtgacta tcatattgtt atatgtagtg aacttgaaaa
      241 tattattgat aaccttaatg ataatcgaat cgttgctatt attatggact tagatgatgc
      301 gactgaagtt aatgtcgcaa agtattctag ttataaaaat attgataata ttcaaatccc
      361 tttaatcgta ggtgggaact ttacgggaaa tcttcttaat ttattaaatt taggagttag
      421 tgaggtaatt gttaagcctt ataatgaagc aattattaaa ttacgaatca ataatgcaat
      481 tcgtcgggcc gaagataatg aatatagaat aatggctgat tttgatactt taaccaagat
      541 ctataaccgt actgcttttt atcgcgaagc taaaaaatta attacgcaaa accaagatat
      601 taattatgat cttgcttgtt ttgatattga tcgatttaag attattaatg atatttatgg
      661 tagtagtgtc ggtgatgaac tattgattta tattgctaac acgggagtta agcggatgaa
      721 gaaacttggc ggattaattg gacgtttaag tggggattta tttgcaatcg tagttcctca
      781 tcaagcgaat attgaagacc tgttgcttca acaaatgaat gaagaccttg gtaactttga
      841 tcttggaatc aaggttgtag tcagttttgg ttattataat attgatgatc tatcattacc
      901 agtcaataat atgtgtgatc gggcaatgat ggcgataaaa aaagtaaaag ataaatacaa
      961 taccagctat gcaacttatg atgatgaact tcgagatcaa gttttagaag aacagagaat
     1021 tattgatgaa atggatcgtg catttgaaaa tgatgaattt attccttatt atcaacccaa
     1081 atttaatatg gtaacgagga catatattgg ttatgaggca ttgatgcgtt ggcagagtcc
     1141 gactcgagga atggtgttac caagtacctt tattccagtt tttgaaaaga atggttttat
     1201 ttctaaagca gatcgggtaa tttggcgtaa agtatgtcaa gatatggtta ttgcccgcca
     1261 aaaaggacat gtcttattac ctgtatctgt aaatgtttct cgaattgaat tatatgatcc
     1321 ggaattagga aatactatct taaaactttt agcggaatat gagctgtcga ttgagttatt
     1381 tcaattagaa attactgaaa cggcatatat gcaggatagt aatcaaatga ttgaggctgt
     1441 agtaaagtta aaggaacttg gcttcactat tttaatggat gattttggta gtggtttttc
     1501 atcgttgaat atcttaaagg aattgccgtt tgatataatt aaaattgatt tggcgttttt
     1561 ggaacatttt gataaaaata ataaagctga aaagatttta aaatcagtga ttcaaatggc
     1621 gaaacgcctt aatatggaag taattgctga aggagtnnnn nnnnnnaaac caaacgccaa
     1681 gaagattttt tagtagaact aggttgtaat cgtgctcaag ggtatcgttt tgctaaacca
     1741 gctagtgctt caaaaattgc ttatatggtt gaaaatggtg tcatcggagt aggtgatact
     1801 aaagatgagg atagtgcaat agttaatatt gatgatattt taacgactgt ttatcagcaa
     1861 ggggaagtag attggtatcg acaggcacta ttagaattaa atgctcaagt tttcgaatat
     1921 gattttaaac gtgataatat gattgttttt gatactccaa caaaggaaaa tggttctaat
     1981 ttagctaaga tggaaattcc caattatctt tacaatgtaa gtattggtag aatcgtacat
     2041 tcaaaagatg taaaaagata tcagactgtt ttcaatggta atcaggaatt taaggttatc
     2101 tatcgacgtt ttatgattaa ccatggttct ggttatactt gggttaaaga tacaggaaga
     2161 ataatttatg atgaagataa caaaccaaaa atatgtattg gtgtaagtcg tgttatttca
     2221 gatgaaaagc ttaatgaaca aatgatgaat gttttatcag taatggagca gtcaactgat
     2281 tttgattcat caatcaacca tattttggca gagatcgggg atgatttttt acttgatcgg
     2341 attacgttat tactagaaga aggaaacaat tatcgttctg tctatgcatg gcaagatgag
     2401 tcgattgagc ttgagattag tgatactttc ccaatagtga ggacagagct tcaagaaata
     2461 gtcacatatt ttaaggagca tcaaataatt gttgttaata aaaatgaagg taatcaattt
     2521 agtaaacaaa ttaataaaaa tttctttgat aatcaagtaa agacacttgt tatcattagt
     2581 ctaaacgatg atattggtga atttatcggg tgtattgttt ttacaatgat aaatgattat
     2641 cgacaatggc gcgatgatga actttgggca ttacaagaat tagtaaaggg gataaatgtc
     2701 tatttaaata aagagaaaat ttttaatcgt ttagatagca tattattaac atataatcgt
     2761 gttatggaaa aattgaatga tggaatattg atgtttacat atgagtctca gccccagctg
     2821 ctatttatta atgaagcata ccgtaagata ttaaatattg atgatataaa tgttactaat
     2881 tttcttagta cgtactatcg gagcgtcgat ttggatgttc aaaagcaaat tcatcaggca
     2941 attgaaaagt gtgttgaaac tggggaagaa cagacgcttt atcatccact taaaacttgt
     3001 gaaaagcaga ttattaaggc taaaactatt tataatcttt cgccagttaa agaaaatggt
     3061 aaattggtaa ttattagcat tactacgaaa cagtgcactt aagtgctatt attttatact
     3121 tgtgtataat tgtatctagg aggtcataat aatggcagaa atattgatta ttgaagacga
     3181 agttaaattg cgccaagaac tggaagtata tttaaataat aatggatatt tgactaaagt
     3241 aattgagtct tttgaaaata ctttagaaca aatgaatcag tttgatgggg atttaatttt
     3301 attggatatt aatcttccca atgtaaatgg acagttttta tgcaaagaat atcgtaagaa
     3361 ttcaagtaaa ccaattatta ttgtaactag tcgcaacaat gagatggatg aattgatgtg
     3421 tattaattat ggtgctgatg attttgtcac gaaaccgtat aatccattaa ttttattggc
     3481 ccgaatcgaa gcggtcttaa aacgaacaca gcctacaagt gatgttttga ttagttatga
     3541 aaagataaaa ttagatgtat ctaaaagtag tattattgat aaaaataaag tgattgaatt
     3601 atcaaaaaat gagttaaaaa tattttactt ttatcacaat aattgcagct ttattgttat
     3661 aaacatcaca ataatttggt gccgagaata c
//
LOCUS       DAOMTA010000218         3690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_874_length_3690_cov_2.411279, whole genome shotgun sequence.
ACCESSION   DAOMTA010000218 DAOMTA010000000
VERSION     DAOMTA010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_3690_cov_2.411279"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLK68_07025"
     CDS             complement(<1..1233)
                     /locus_tag="PLK68_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90198.1"
                     /translation="MKGIMNWFTNKLAPGMQKVFSNPWIAAVASAMQKILPFILVGSV
                     ISIYNVFVRYIPSLPDLSFVNTFSFGMMSLIVAFMVTYFGMVELDHPKYTITAGLTSV
                     TVFLMALCPTMATLLKNATTGKTELTFTDINFLGGSGLFIAIIVGLVVMLIFHLYAKL
                     HILEDSATMPDFVCEWINNIVPMTIIYLIFGVTVFTIGFDLVEFINLIFQPIVNLGQS
                     LLGFVVICFLYVLLYSVGISAWSLNAIVKPILMAGIAANAEIALAGGSPIYIVTTETI
                     FTTALIAMGGLGGTLPLNFLMLFKCKSKKLKTMGKICIGPSIFNINEPLVYGAPIVFN
                     PLLMIPMWINSIIGPLIIWFVMKAGMLNIPAGVNNISRIPAPICTWLTTDDFRAFIWF
                     LVLFIIYGLIWYPFLMRYD"
     gene            complement(1257..1580)
                     /locus_tag="PLK68_07030"
     CDS             complement(1257..1580)
                     /locus_tag="PLK68_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM90199.1"
                     /translation="MAQAHIYLCCGGGLSSGFLAQKARAAAKKGKIDATIEAKSESEV
                     TQYLPKMDILCIGPHYEFRLNAFKDMAAPYNIPVIVIPEEIYSMLDGKALLELALLEI
                     EEFYK"
     gene            1775..3688
                     /locus_tag="PLK68_07035"
     CDS             1775..3688
                     /locus_tag="PLK68_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM90200.1"
                     /translation="MRRKQEELINYLYTHNEKVTANILSKALNLSIRTIKSYIAELNM
                     NYPSLISSSNRGYVIDKVKANSLLQYKDDIPQDYESRCIYIIKKTLLEKQDYIDIFDL
                     CEELFISYSTLKKDIYKMNTSFANFKITFSSENNKLHVGGSEQNKRKLISHVMSEEVS
                     GNFLNLTLLQESFPDYDLDDACTLIKDICKQHHYYLNDFSCVNFILHVTIMVSRINHG
                     NHIINNNELIQVTNKNDEKIAKELCLALEQVFNVSFNSSEILEIYILFKNNANYINDE
                     NENVSLLVSDEIIQITKNIIKNVDEHFFINLDSDNFITPFMLHLKNLKNRLIKNNLLK
                     NPMLDSIKISCPTIYDISTFIAYQLTLSFHENVNEDEIAFIALHVGTEIERQKKEETK
                     VSCLLLCPEYLNITSTLHKKIMMDFGDQLTIQKSISFENEILGNNFDLLITTVPVLES
                     TNYFTVLLPPFPMSYEKNKILDAIIRIENTKKSQILTNNLNFYFNEKLFYSMNEDISK
                     SAVINELAERMINLGYVEENFKEEIWKRETASSTAFMNIAIPHPMKMSAYKTSIGVVI
                     SHKGIDWGNQHFVNVVFMIAFNKIDNKHFHALYESLVLLFNEPIVISEIKKCKNFNDF
                     KDIVIKNYLKFNE"
BASE COUNT         1400 a          597 c          477 g         1216 t
ORIGIN      
        1 atcatatctc attaaaaatg gataccagat caatccataa ataataaata gtacaagaaa
       61 ccatataaac gctcggaaat catcagtagt taaccaggtg caaatcggcg caggaatacg
      121 agaaatatta ttaactccgg ctggaatatt tagcatacca gctttcatta caaaccagat
      181 aattaatgga ccaataattg aatttatcca cattggaatc attagtaacg gattaaagac
      241 aattggcgcc ccataaacaa gtggctcatt aatattaaat attgacggtc caatacagat
      301 tttccccatt gtttttaatt ttttagattt acatttaaat aacattagaa aatttaatgg
      361 taatgttcca cctaagcccc ccattgcgat taaggccgtt gtaaaaatcg tttctgttgt
      421 cacaatataa attggacttc ctccagccaa tgctatctca gcattggctg caattcccgc
      481 cattaaaatt ggtttaacaa tagcatttaa cgaccaagct gaaataccta ctgaatataa
      541 caatacgtat aaaaaacaaa taacaacaaa tcccagtaaa gattgtccta aattaacaat
      601 cggctggaat attaaattaa taaattctac taaatcaaaa cctattgtaa atacagttac
      661 tccaaatatt aaatagataa ttgtcattgg aacaatatta tttatccatt cacaaacaaa
      721 atccggcatt gtagcactat cttccaaaat atgtaacttt gcatataaat ggaaaatcaa
      781 cattacaact aatcctacaa taatcgcaat aaataaacct gatccaccta aaaaattaat
      841 atctgtaaaa gtaagttcag ttttcccagt tgttgcattt tttaataagg ttgccatggt
      901 tggacataag gccattaaaa atacagtcac tgaagttaac cctgcagtta ttgtatattt
      961 aggatgatcc agttccacca tcccaaaata agtaaccata aaagctacta tcaatgacat
     1021 cattccaaag ctaaatgtat taacaaaact caagtccggt agtgatggaa tgtaacgtac
     1081 aaatacattg taaattgaaa taactgatcc taccaaaata aacggcaaga ttttctgcat
     1141 tgcgctcgcc actgctgcaa tccaaggatt actgaaaact ttttgcatcc ctggtgctaa
     1201 tttattagta aaccaattca taattccttt catttttctc tctcctgtta tatattttat
     1261 ttataaaact cttctatttc taataatgct aattcaagta atgcttttcc atctaacatt
     1321 gaataaattt cttcaggaat aacaattaca ggaatattat atggggctgc catatcttta
     1381 aaagcattca gcctaaattc ataatgcggt ccaatgcaaa gaatatccat ttttggcaaa
     1441 tattgtgtga cttcgctttc actctttgct tcaatagttg catctatttt accttttttt
     1501 gcagctgctc gagctttttg agctaaaaat cctgaagaga gccctcctcc acaacaaaga
     1561 taaatatgtg cttgtgccat ttttatttcc tccatttctt atttgtctgc aagttcttaa
     1621 actttacgct tatatttaat cattttgttc atatctactc aactaacatt ctgcacagtg
     1681 caaataagta ttttgcactg tgcaaaattc tagttttaaa taaaaattaa aactattata
     1741 atatagaaaa caacaactag ggaaggatga gagcatgaga aggaagcaag aagaattgat
     1801 caattattta tatactcata atgaaaaagt tacagcaaat attctttcaa aagcattaaa
     1861 tctctctatt cgaacaatta aatcatatat tgccgaatta aacatgaatt atcctagctt
     1921 gatttcttct agcaatcgtg gttatgtcat cgataaagta aaagctaact cactacttca
     1981 atataaagac gatattcctc aggactatga aagcagatgt atttatatca taaaaaagac
     2041 gcttttagaa aagcaggact atattgatat ttttgattta tgtgaggaat tattcattag
     2101 ctatagtact ttaaaaaaag atatttataa aatgaatact tcatttgcaa attttaaaat
     2161 tactttttct agtgaaaata ataaacttca tgttggaggt tctgaacaaa ataaacgtaa
     2221 actaattagc catgtgatgt cagaagaagt aagcggaaat tttcttaact taactttgct
     2281 acaagaaagt ttcccagatt atgatcttga tgatgcctgc accctaatca aagatatttg
     2341 caaacagcat cactactatc ttaacgattt ttcttgtgtt aattttattt tgcatgtaac
     2401 tattatggtt agtcgaatca accatggtaa tcacattatt aacaataatg aattaattca
     2461 agttactaat aaaaatgatg aaaaaatagc taaagaacta tgcctagcct tagaacaagt
     2521 ctttaatgtc tcttttaatt catcagaaat acttgaaatc tatattctct ttaaaaataa
     2581 tgcaaattat ataaatgatg aaaacgaaaa tgtttcttta ttagtatcag atgaaattat
     2641 tcaaattacc aaaaatatca taaaaaatgt tgatgaacat ttctttatta atcttgactc
     2701 cgataatttt ataactcctt ttatgctaca tctaaaaaac cttaaaaata gattaataaa
     2761 aaacaatctt cttaaaaatc caatgcttga ttctattaaa atatcatgcc caactatata
     2821 tgatatctca acgtttattg cttatcagct gacattatca tttcatgaaa acgtcaatga
     2881 ggatgaaata gccttcattg ctttacatgt aggaacagag atagaaagac aaaaaaaaga
     2941 agaaacaaaa gtatcctgtc tcctattatg tcctgaatat ttaaatatca ctagtacttt
     3001 acacaaaaaa attatgatgg attttggaga tcaattaact attcaaaaat caatttcatt
     3061 tgaaaatgag atcctaggga ataattttga tcttcttatc actactgttc ctgttttaga
     3121 atctacaaac tactttaccg tattattacc accattccca atgagctatg agaaaaataa
     3181 gattttagat gcaatcatta gaatagagaa cactaaaaaa agtcaaattc ttactaataa
     3241 tcttaatttt tattttaatg aaaaactttt ttattcaatg aatgaagata ttagtaaatc
     3301 cgctgttatt aacgaactag cagaacgaat gattaatcta ggctatgtag aagaaaactt
     3361 taaagaagag atatggaaaa gagaaacagc aagttctaca gcttttatga atattgccat
     3421 tccacatcct atgaaaatga gcgcttataa aactagtata ggagtggtaa ttagtcataa
     3481 aggaattgac tggggaaacc agcattttgt caatgtagtt tttatgattg cttttaataa
     3541 aatagataac aaacattttc atgcattgta tgaatctcta gtattattat ttaatgaacc
     3601 aattgtaata tcagaaataa aaaaatgtaa gaactttaat gattttaaag atatcgttat
     3661 aaaaaactat ttaaaattca atgaatgata
//
LOCUS       DAOMTA010000219         3680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_875_length_3680_cov_2.451586, whole genome shotgun sequence.
ACCESSION   DAOMTA010000219 DAOMTA010000000
VERSION     DAOMTA010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_875_length_3680_cov_2.451586"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..2052)
                     /locus_tag="PLK68_07040"
     CDS             complement(139..2052)
                     /locus_tag="PLK68_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM90201.1"
                     /translation="MNKLFEKSYIGKMKLKNRIVMGPMGTTGEADGAYNIDAINYFTE
                     RAKGGTGLIITGANVVSTKYEPRPCTELSNFHHVERLNMLVERCHNYGAKVCVQVSPG
                     LGRQQFTDPFTPPYSASDCGAFWFPDLKCKPFTKEDIKYIVEKVGYGASLVKMAGADA
                     VELHAYGGYLLDQFHSKQWNTRTDEYGGSLENRMRFTLECIAAIRTNVGPDFPILVKF
                     TPVHKVEGFRQIDEGIAMAKILEGAGIEALHVDVGCYEAWYKAISTVYNEEGHQLDVV
                     KAIKENVSLPVLGQGKLFDPKKAQEVVAQGVLDYVVLGHQMLADPHWANKVKQGNTMD
                     IAPCVGCNECLLAGFSGKHYYCAVNPLCYAEKEFALPKVDGIKKSVLVIGGGPGGMYA
                     AITARKRGYEVDLYEKEERLGGTLWAAGMPTFKHDVLKLITYLERQCLKTGVNVYLNT
                     EFTLKDAKKDYDKVILSAGSSPMMPPIPGIEKAGFVSEFLTDRKEAGKQIVIIGGGLA
                     GCEAACYLKENAETVTIIEMQEDILFGCDHCLNNDQALRQMLQDNNINKITKATVTKI
                     EDNRVTYLQDNQEYTIVCDTIINAAGFKPNDQLEDALEEIYDDVTVIGDAKAPRKILT
                     AIHEGYHAIRVME"
     gene            2201..2773
                     /locus_tag="PLK68_07045"
     CDS             2201..2773
                     /locus_tag="PLK68_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM90202.1"
                     /translation="MTKITKDIIIETAYQLFFKHGYNNVTINDICKECKITKPTFYTY
                     IKSKDDILAHFYDDITEAIVANTANIIMAENYWQQLLICFETLMEESIKLGYDLSSQM
                     FIMNLKEDRGSFDFRDHLTNIAIAIIKKGQATKQIRNQNDPEILYQASAYAFTGYELM
                     WCIKKGQFDWKKELRIALENIYDVAPELRG"
     gene            2776..>3301
                     /locus_tag="PLK68_07050"
     CDS             2776..>3301
                     /locus_tag="PLK68_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM90203.1"
                     /translation="MKLYNTIKINNHSYYINDEDGDSCYLIIGNTHALLIDLGLFKEP
                     LLPTIKNITNKELIIVCTHGHFDHIGTIKEFKEETIYLSHRDRDIYYDNAHIIKELSL
                     IDFNQIKDLKNHQQIELGNFEIEVLALPGHTPGSMIFLDRQNKCIYTGDAIGSGCGVW
                     LQLFHSLDLKTYHTA"
     assembly_gap    3302..3311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3312..3580
                     /locus_tag="PLK68_07055"
     CDS             <3312..3580
                     /locus_tag="PLK68_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM90204.1"
                     /translation="QTIDYLEKQGVDDTWHFWGGHNQQEIQSKISAYNKLDFVLMKDL
                     EQLCLKLINSQITGIKNSAPTFDDNQAYYASYGKAEIIYQKNSL"
BASE COUNT         1223 a          695 c          504 g         1248 t
ORIGIN      
        1 aagcttcttt tgtatcttca ggaataatat tttttactgc taaagtccca tcaatatcaa
       61 aaaagaatat ttttttactc ataatagttc ctccattaca ttattataca ataaaaataa
      121 agtagagttc aatgaactct actccataac tcgaattgca tgatatcctt catgaattgc
      181 tgttaaaatt tttcttggtg cttttgcatc accaatcact gtaacatcat cataaatttc
      241 ctctaaagca tcttctaatt gatcatttgg tttaaatcca gctgcattga taattgtatc
      301 acacacgatt gtatactcct gattatcttg gagatatgta actcgattat cttcaatttt
      361 agtaacagtt gcttttgtaa ttttattaat attattatct tgaagcattt gtctcaacgc
      421 ctgatcatta tttaaacagt ggtcacaacc aaataaaata tcttcctgca tttcaataat
      481 agttaccgtt tccgcatttt ctttaagata acatgctgcc tcacatcctg ctagaccccc
      541 accaataatt actatttgtt ttcctgcttc tttacggtca gtcaaaaatt cacttacaaa
      601 acctgctttt tcaattcccg gaattggtgg catcattggt gatgaccctg ctgatagaat
      661 aactttatcg taatcttttt tagcgtcttt gagcgtaaac tctgtattta aataaacatt
      721 cacaccagtt ttcaagcatt gtctttcaag ataagtaatc aatttcaaga catcatgctt
      781 aaaggtaggc attcctgccg cccaaagtgt accccctaat cgttcttcct tttcatataa
      841 gtcgacttca tatccacgtt ttcttgctgt gatagcagcg tacattccac ctggaccgcc
      901 tccaataact agtactgatt ttttaattcc atcaactttt ggcagtgcga attctttttc
      961 cgcatagcaa agtggattta cagcacaata ataatgtttt ccagaaaaac ctgctaataa
     1021 acattcatta cagccaacgc atggtgcaat atccattgta tttccctgct ttactttatt
     1081 agcccaatgt ggatcagcaa gcatttgatg acctaaaaca acataatcca aaactccttg
     1141 tgctactact tcttgtgctt tttttggatc aaataacttt ccttgcccca atactggcaa
     1201 tgatacattt tctttaattg ctttaactac atctaattgg tgtccttctt cgttgtaaac
     1261 agttgaaata gctttatacc aagcctcata acatcctaca tcaacatgta atgcttcaat
     1321 ccctgcaccc tcaagaattt tagccatagc aattccttca tcaatttgtc taaatccctc
     1381 aactttatga accggagtaa atttaactaa aattggaaaa tccggtccta catttgttct
     1441 aatcgcggca atacattcaa gcgtaaaacg cattctattt tctaatgaac cgccatattc
     1501 atcagtacgt gtattccatt gttttgaatg aaattgatct aataaatatc cgccgtaagc
     1561 gtgtaattcc accgcatctg ctcctgccat ttttactaaa gaagcaccat atccaacctt
     1621 ttcaacgata tattttatat cttcttttgt aaatggctta catttcaaat caggaaacca
     1681 aaacgcccca caatcactag ctgaatatgg tggtgtaaat ggatcagtaa attgttgtct
     1741 tcccaatcct ggtgatactt gaacacaaac tttagcgcca tagttatgac atctttctac
     1801 taacatattc aaacgctcaa catgatgaaa attactaagt tccgtacatg gccgaggttc
     1861 atatttagtg gatacaacat ttgcaccagt tataattaag ccggtacctc cctttgccct
     1921 ttcagtaaaa taattaattg catcgatatt atatgcccca tctgcttcac cagttgttcc
     1981 catcggtccc atcacgatcc gattttttaa tttcatttta ccgatataac ttttttcaaa
     2041 taatttattc ataattctcc tcctttactt cggtaatatt tattttactc aagtaaagta
     2101 taacacaata atcataaatg taaagggtta catttatgat tacattactt gcttttttta
     2161 ctagatgatt tatacttaat tattagaaat gaggaatacg atgacaaaga ttacaaaaga
     2221 tattattatt gaaacagctt accaactgtt tttcaaacat ggatataata atgttacaat
     2281 caacgatatt tgtaaagaat gtaaaatcac aaaaccaact ttttatacct atataaaatc
     2341 taaagacgat attttagcac atttttatga tgacattaca gaagcaatcg ttgctaatac
     2401 ggctaatata atcatggcag aaaattattg gcaacagtta ttgatctgtt ttgaaacatt
     2461 aatggaagaa tcaataaaac tcggctatga cttaagtagt caaatgttta ttatgaattt
     2521 aaaagaagac cgtggtagtt ttgattttcg agatcatttg accaatatcg ctattgcaat
     2581 tataaaaaaa ggtcaagcca ccaaacaaat tagaaatcaa aacgatcctg aaatactata
     2641 tcaagcttca gcatatgcat ttacaggtta tgaattaatg tggtgcataa aaaaaggtca
     2701 gtttgactgg aaaaaagaat taagaatagc attagaaaac atatatgatg ttgcaccaga
     2761 acttcgaggt taaatatgaa actatataat accataaaaa ttaacaatca cagctattat
     2821 atcaatgatg aggatggtga ctcttgttat ctaataatag gaaataccca tgctctatta
     2881 attgatttag gcctatttaa agaaccactt ttaccgacga ttaaaaatat tactaataaa
     2941 gaattaatta tagtatgtac tcatggtcac tttgatcata ttggcacaat caaagaattt
     3001 aaagaagaaa ctatctattt atctcatcga gaccgtgata tttattatga taatgctcat
     3061 attattaagg aactttcttt gattgacttc aaccaaatca aagatttaaa aaatcatcag
     3121 caaattgagc ttggtaactt tgaaatagaa gtccttgctc ttcctggtca caccccggga
     3181 tcaatgattt ttctagaccg tcaaaataag tgtatttaca ctggtgatgc aattggttcc
     3241 ggctgtggtg tttggctaca attattccat agccttgatt taaaaacgta tcataccgct
     3301 cnnnnnnnnn nagcagacta ttgactatct tgaaaaacaa ggagtagacg atacttggca
     3361 tttttggggt ggacacaatc aacaagaaat tcagtcaaaa atttctgcat ataataagtt
     3421 agattttgtt ttaatgaaag atcttgaaca actatgccta aaattaataa acagtcagat
     3481 tactggtata aaaaactcag ctccaacatt tgatgacaac caagcctact acgccagcta
     3541 tggtaaagct gagataattt atcaaaaaaa tagtttatag tgaaattttc ggatttcact
     3601 ttcttttttc attcgcatta gagctattat ctatgataaa ataattccat atggaggttt
     3661 accaatgaaa acattacatg
//
LOCUS       DAOMTA010000220         3680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_876_length_3680_cov_1.867586, whole genome shotgun sequence.
ACCESSION   DAOMTA010000220 DAOMTA010000000
VERSION     DAOMTA010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_3680_cov_1.867586"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLK68_07060"
     CDS             complement(<1..503)
                     /locus_tag="PLK68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM90205.1"
                     /translation="MKIAENLFNYFKNTGTLIKYQPNDIIYMQEDSSNNLYLILKGRV
                     RVYLISKDGQEITLDIIDKGRIFGESSFLLNTSRPVCVSAIDEVELISCNLENLYPAI
                     LESKELTIAIMQLLSSTNDHLTNQVKKAYFYNRHEKIAAFLLEQKKKTISYTHEEIAS
                     LTGLNRVT"
     gene            589..1437
                     /locus_tag="PLK68_07065"
     CDS             589..1437
                     /locus_tag="PLK68_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM90206.1"
                     /translation="MKGKLLRIGTIILGNVFVAFAVSTLVLENQLISGGVTGLGIVTN
                     HYTGINISLIVGIINVLLFLLGLFFIGKKFALSTLISTFAFPVLLEFFNTNAIFHNYC
                     QDTLLAVVLAGCLVGIGVGLMIRSDASSGGMDIIAIILNRKLGIPVFIMVNVFDFIIL
                     CMQATFSNPTKILYSIVLVFVTSFMLNKTLTKGSKMVQLVVISDCHEMIKKMIIEEAD
                     AGVTSLYSQKGFNETDTKTLLTIIPPVKLTKIKEQIKLIDPVAFMVVATVDEVSGRGY
                     TLERHH"
     gene            complement(1420..1827)
                     /locus_tag="PLK68_07070"
     CDS             complement(1420..1827)
                     /locus_tag="PLK68_07070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM90207.1"
                     /translation="MKRNVNIIVVFNQNKSKVLMCKRKKAPFKGLYNFVGGKIEPNED
                     HLQAAYRELAEETNISKTDIELIHFMDFTYYLDDTLLETYIGTLSTNVDIHGEENQLA
                     WIKLTDDFKDLNRFAGNGNIYHILKEISLMMPF"
     gene            1941..2765
                     /locus_tag="PLK68_07075"
     CDS             1941..2765
                     /locus_tag="PLK68_07075"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRM90208.1"
                     /translation="MEIIELIKVIILGIVEGITEWLPISSTGHMILVDEFIKLNVSAD
                     FLEMFLVVIQLGAILAVVVLYWKKLIPFDYKNRWRIKKDTFSMWFKIIFACLPAAVVG
                     LLFDDQLNELFYNPLTVAIALIVFGVLFIFIENYNKGKETKINSLSEITYNTALIIGI
                     FQLIAAVFPGTSRSGATIVGALLIGVSRTVAAEFTFFLAIPVMFGASLLKLVKFGFAF
                     TTAELLLLVIGMLVAFVVSMLTIKFLMSYIKKHDFKAFGWYRIILGIIVLIYFMVF"
     gene            complement(2899..3246)
                     /locus_tag="PLK68_07080"
     CDS             complement(2899..3246)
                     /locus_tag="PLK68_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRM90209.1"
                     /translation="MDIQGKEVYLYFHCAMEGKKLDLIAKDNRVTFEMDCDHNFILYE
                     ERMSCTMGYESVIGHGVIETVPDENKYESLKILMRQYHAEDFKFNTDMMRVTTVLKMT
                     VIDMVGKRRNNIH"
     gene            complement(3183..3341)
                     /locus_tag="PLK68_07085"
     CDS             complement(3183..3341)
                     /locus_tag="PLK68_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90210.1"
                     /translation="MRRKDREITDFNEIINIIKKCDVCRLFHSTLDWISREKKYIYIF
                     IVPWKEKN"
BASE COUNT         1172 a          500 c          581 g         1427 t
ORIGIN      
        1 gtaacgcgat taagtcctgt taaagaggct atttcttcat gtgtgtatga gatagtcttt
       61 ttcttttgtt ctaataaaaa agcagcaatt ttttcatggc ggttataaaa atatgctttt
      121 tttacttggt tagtaagatg atcattagtt gaagataaga gctgcatgat tgctatagtt
      181 agttcttttg attctaaaat ggcaggatac aaattttcaa gattacaaga gatcaattca
      241 acttcatcaa tagcactaac acaaactggt cttgaagtgt ttaataaaaa tgaagattcg
      301 ccaaagattc ttcctttatc gatgatatct aaagtgattt cttgtccgtc ttttgaaatt
      361 aaataaacac gaacacgtcc ttttaaaatc aaataaagat tgttgcttga atcttcttgc
      421 atatagataa tatcgttggg ctgatatttg atcaatgtac ctgtattttt aaagtaatta
      481 aagagatttt ctgctatttt catatttttt ccaccttttt tcttaattgt agcatatgtt
      541 acattctttg gtaaataatt atgctatctt ataccccgag gtgataatat gaaaggaaaa
      601 ttattaagaa tcggaactat tatattagga aatgtttttg tagcttttgc ggtaagtact
      661 ctggttttag aaaatcaatt aatttctgga ggagtaactg gtctaggaat cgttactaat
      721 cattatactg ggataaatat ttcacttatc gtaggaatca ttaatgtttt attattttta
      781 ttaggattat tctttattgg taaaaaattt gctttatcta cacttatatc aacatttgct
      841 tttcctgttt tgctagaatt tttcaatacc aatgcaattt tccataatta ttgtcaagat
      901 actttattag cagtcgtttt agcaggctgc ttagttggaa ttggggtagg attaatgatc
      961 cgttcagatg catcatctgg aggtatggat attattgcga ttatcttgaa tagaaaatta
     1021 ggaattccag tattcattat ggttaacgta tttgatttta ttatcttatg tatgcaagca
     1081 accttcagta atccaactaa aatcttatac agtattgttc ttgtttttgt aacatcattt
     1141 atgttgaata aaactttaac aaaaggttct aagatggttc aattagtagt aattagcgac
     1201 tgccatgaaa tgatcaaaaa aatgattatt gaagaagcgg atgctggggt aacttcttta
     1261 tatagtcaaa aaggttttaa tgagactgac actaaaacct tattaacaat tattccaccg
     1321 gttaaattaa ccaaaatcaa agaacagatt aaattaattg atcctgttgc atttatggta
     1381 gtagcaacgg ttgatgaagt tagtggccga ggatatacat tagaacggca tcattagtga
     1441 tatttctttg agaatatggt aaatattacc atttcctgca aaacgattaa gatctttaaa
     1501 atcatcagtt agcttgatcc aggctaactg attttcttca ccgtgaatat caacattggt
     1561 tgaaagggta ccaatatatg tttctagaag agtatcatca aggtaatagg taaaatccat
     1621 aaaatgaatc agttcaatat ctgttttaga tatattagtt tcctcggcta attcacgata
     1681 tgctgcctga agatgatctt catttggctc gatttttcct ccgacaaaat tataaagtcc
     1741 tttaaaagga gctttttttc ttttacacat taatactttt gatttatttt gattaaatac
     1801 gacaattatg tttacatttc ttttcatttc atttttcctc tgcgtatatt ctatcatatt
     1861 aataaaatct taagaatata tttaatgtaa tttagttaag atagtgctat aatgcactag
     1921 tataattggt ggaggagagt atggaaatta ttgaattaat taaagttatt attttaggaa
     1981 ttgttgaagg tattactgaa tggctaccaa ttagtagtac tggacatatg attctagttg
     2041 atgaatttat taaattaaat gttagtgctg attttttaga aatgtttcta gttgtaattc
     2101 agctaggagc aattttggca gtagttgtac tttattggaa aaagttgata ccttttgatt
     2161 ataaaaatag atggagaata aagaaggata ctttttcaat gtggtttaaa attatttttg
     2221 catgccttcc agcagctgta gtaggtcttt tgtttgatga tcaattaaat gagttgtttt
     2281 acaatccttt gacagttgca attgcattga ttgtttttgg ggttctgttt attttcattg
     2341 aaaattacaa caaaggtaaa gaaacaaaaa tcaattcatt aagtgaaatt acttataata
     2401 cagccttgat cattggaatc tttcaattga ttgctgcggt atttcctggt acatcacgct
     2461 caggagcaac gatcgttggg gctttattga ttggagttag tcgaactgtt gcagcagaat
     2521 tcactttctt tttagcaatt ccagtaatgt ttggagccag tttattgaaa ttagttaaat
     2581 ttggttttgc ttttacaaca gcagaattgt tattattagt gattggaatg ctagtagctt
     2641 ttgtggtcag tatgttaacg atcaaatttt taatgagcta tattaaaaaa catgatttca
     2701 aagcatttgg ctggtatcga atcattttag gaattattgt tttaatatat tttatggtat
     2761 tttaaaagct gaaggattgt agctatgtta caacttttca gtttttttat aggtgaaaat
     2821 ttattatcaa gcaaagtaaa agagtataag tttactattt taacttatac tttttgaatt
     2881 ctatattttt ctattatttt aatgtatatt atttcttctt tttccaacca tgtctattac
     2941 tgtcattttt aatacagttg taaccctcat catatcggta ttaaatttaa aatcttcggc
     3001 atgatattgt cgcattagaa tctttaatga ttcatattta ttctcgtcgg gaacagtttc
     3061 aataacacca tgaccaataa cactttcata tcccattgtg cagctcattc tttcttcata
     3121 aagaataaaa ttgtgatcac aatccatttc aaatgttaca cggttatctt tcgcaatcaa
     3181 gtctaatttt tttccttcca tggcacaatg aaaatataaa tatacttctt ttccctggat
     3241 atccaatcca aagttgagtg gaacaatcta caaacatcac attttttaat aatgttaatg
     3301 atttcattaa aatctgtaat ctctctatct tttcttctca taatattctc cttatcaaaa
     3361 tcatatttgt ctgtttaatt ttatgttata gtatgatatt tattgaatgg gtcttttttc
     3421 tagatttgaa aatatattta ttattttgga taaaataaaa accgctctag aaagcggttt
     3481 atagacttca tggcatcctc agtaggtcaa agcaactata ttttacatac aattacacgc
     3541 tattacatga cgctatatca aaggttttaa ggcacatact agggtacatg ccagtacgtg
     3601 cctttttatt tgacactcaa aaagtgtcga aaatgtggcg agtttaagca tcaatatatt
     3661 tatataatac aattttcccc
//
LOCUS       DAOMTA010000221         3672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_882_length_3672_cov_1.984794, whole genome shotgun sequence.
ACCESSION   DAOMTA010000221 DAOMTA010000000
VERSION     DAOMTA010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_3672_cov_1.984794"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>944)
                     /gene="mltG"
                     /locus_tag="PLK68_07090"
     CDS             complement(<1..>944)
                     /gene="mltG"
                     /locus_tag="PLK68_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRM90211.1"
                     /translation="FFYFNGQGAVSSKSEEVVVEISGSTSSVLNQLDKAGLLKSKTVA
                     SIYTKFNSYSFKANVYVLNKNMDLKKILTILEGDKDYISAAKITILDGYRIPECAQQV
                     AKGLEIDSTEVLEKWTNKEYLQTLVEKYWFLDESILGADIMFPLEGYFGPETYVITSK
                     KTSIEDVTKMMLDQMDGNLSTYKDKISNFMISGNKVSMHQFLSLASVVQCESSGQKED
                     QAKIAGVFMNRLEKPMRLQSDVTVNYANQIKTVAVTYNHLSVDSKYNTYKYEGLPVGP
                     ISTVSTNIIEACLNYQKTDNLFFFALKDGSVIYSKTYE"
     assembly_gap    945..954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1095..2186)
                     /locus_tag="PLK68_07095"
     CDS             complement(1095..2186)
                     /locus_tag="PLK68_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HRM90212.1"
                     /translation="MALMGLFGKKRVTESFFVNSYGNDQTRGIYTFQVDIENGEILYK
                     KHFKTPSDPVYSFNYGRFVCVTYKNRTGTSGDGGICSYAATADILALVSRISDKGKTY
                     MHACANGDHETADKLYAVDYYNGEIMVSKIDKKKLVAPIFNYKLEGHSIDPKRQNQPH
                     PHFVDFTPDGKRLIVVDLGLDKVLLFKMEAKEIILDEEHSFDLEPGSGPKKIMFNQAG
                     TIAYVLNELSNTICVYKYNDLKFELIQTIDTYPKDEYDEPSLAGQMLFSEKEERIFVT
                     NRGHDSLALFLVDQETGLLTYKDFVDTSPNARDIAIFKDRWIVAVCQKGGVVESYEYR
                     DERGGMLFETKYSYLVSEPVCITKFETIY"
     gene            complement(2241..2588)
                     /locus_tag="PLK68_07100"
     CDS             complement(2241..2588)
                     /locus_tag="PLK68_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRM90213.1"
                     /translation="MEANKIQVIDDQGNEKEFEVLFTFNNEELGKRYVLYYDTTVEEP
                     SVFASIYDDAGQLFPIETPEEWEMVEEVFQSFMAESEEGHECCGNHGNGCCHDNDEEH
                     ECCGNHGDCNCDN"
     gene            complement(2601..3020)
                     /gene="ruvX"
                     /locus_tag="PLK68_07105"
     CDS             complement(2601..3020)
                     /gene="ruvX"
                     /locus_tag="PLK68_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRM90214.1"
                     /translation="MERILGLDLGSRTCGIAISDTLGMLAHGIETYRFRDDDYDRAAK
                     HVVALINEHQIKTVVLGLPKHMNGDLGERAQISIMFKEMLEKEVPGLNVVLIDERLTT
                     KVAQDQLIFADVSRKKRKQVIDKMAAVAILQGYLDAQ"
     gene            complement(3022..3273)
                     /locus_tag="PLK68_07110"
     CDS             complement(3022..3273)
                     /locus_tag="PLK68_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HRM90215.1"
                     /translation="MQDLTKTKVFSSEDMRREMIRQNLKTVIDALNERGYNAVHQIAG
                     YLISNDPAYISSHKNARNIIQQIERDEIIEELVKSYLEK"
     assembly_gap    3318..3341
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3355..>3672)
                     /locus_tag="PLK68_07115"
     CDS             complement(3355..>3672)
                     /locus_tag="PLK68_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRM90216.1"
                     /translation="EINGVKVLFVEEMIDAAKAKQLAFDFRDKIEEGIVILVTQFEDK
                     CSYFVGVTKNYVATGYKAGDIIKKINAVVDGRGGGKPDFAQGGCPINDKISNIKGELK
                     NFF"
BASE COUNT         1146 a          740 c          451 g         1301 t
ORIGIN      
        1 cttcatatgt ttttgaatag atgactgacc cgtctttcaa agcaaagaag aacaaattat
       61 cagtcttttg ataattcaga catgcttcaa taatgtttgt tgaaactgta ctgattggac
      121 cgactggtaa cccttcatac ttatatgtat tatatttaga atcgactgat aagtgattat
      181 acgttactgc taccgtttta atttgatttg cataatttac agttacatca ctttgcagac
      241 gcattggttt ttccaaacga ttcataaaga caccggctat cttagcctga tcttcttttt
      301 ggccagatga ctcacattgt acaactgaag ctaatgataa aaattgatgc atcgatactt
      361 tattaccact aatcataaaa ttactgattt tgtctttata agtacttaaa ttaccatcca
      421 tttgatccag catcatcttt gtaacatctt caatcgatgt cttctttgaa gtaattacat
      481 atgtttctgg accaaaatac ccttctaacg ggaacataat atcagcaccc aaaatacttt
      541 catccaaaaa ccagtatttt tccactaaag tttgcaaata ttctttattt gtccatttct
      601 ctaatacctc ggtactatcg atttctaatc cctttgctac ttgttgagca cattcaggta
      661 tacggtatcc atctaatatc gtaattttag cagcagatat ataatcctta tctccttcca
      721 aaattgtaag aatcttcttt aaatccatat ttttatttaa aacataaaca ttcgctttaa
      781 aactataact attaaacttt gtatagatac tagcaactgt tttgcttttt aatagacctg
      841 ccttatcaag ctgatttaag accgaagatg ttgatccaga tatttcaact actacctctt
      901 cacttttaga agaaacagca ccttggccat tgaaataaaa aaacnnnnnn nnnnaataat
      961 tttctgagtt tttttcatat tctcctccgt cttaaccatt atatgatatt tttagcgctt
     1021 attcaactta tctaccgata ataaaacatg cttcgtcagt atattatgcc aaaaaaagag
     1081 atgaataatc atctctaata aatagtctca aattttgtaa tgcaaaccgg ctcactgact
     1141 agatatgagt atttggtttc aaaaagcatt ccgcctctct catcgcggta ttcataactt
     1201 tcaactactc cacccttttg gcaaactgca acaatccaac gatctttaaa gattgcaata
     1261 tcacgagcat ttggtgaagt atcaacaaaa tctttataag tcaataaacc tgtttcttga
     1321 tctactaaaa ataatgctaa actatcatga ccacgattag taacaaaaat acgctcctct
     1381 ttttcgctaa aaagcatttg tccagctaag ctaggctcat catattcatc ttttggatat
     1441 gtatcgatgg tttgaattaa ttcaaatttc aaatcattat atttgtaaac acaaattgta
     1501 ttagataatt catttaaaac atacgctatt gttccagcct ggttgaacat aatcttttta
     1561 ggtccagatc ctggttctaa atcaaatgaa tgttcttcat ccaaaataat ctcctttgct
     1621 tccattttaa agagcagtac tttatctaat cctaagtcaa caacaattaa acgttttcca
     1681 tccggagtaa aatcgacaaa atgagggtgt ggttgatttt gacgttttgg atcgatacta
     1741 tgtccttcca atttataatt aaaaataggt gcaactagtt ttttcttatc aatttttgac
     1801 accatgatct caccgttata ataatcaaca gcatataatt tatctgctgt ttcatgatca
     1861 ccattagcac aagcatgcat atatgtttta cctttatcac taattcgcga tactaatgct
     1921 aaaatatcag cagttgctgc atatgaacaa attccaccat caccacttgt acctgttcta
     1981 tttttatatg taacacacac aaagcgccca taattaaagc tatagacagg gtctgagggt
     2041 gttttaaaat gctttttata aagtatttct ccattttcaa tatctacttg aaatgtataa
     2101 ataccacgtg tctgatcatt tccatatgaa ttgacaaaaa atgattcagt aactcttttt
     2161 tttccaaata atcccattaa agccatttct ataccacctt ttctagttta agagaaaaaa
     2221 gctaaataat ttagctttta ttagttatcg cagttacagt ctccatgatt accgcaacat
     2281 tcatgttctt catcattatc atgacagcat ccatttccat ggtttccaca acattcatga
     2341 ccttcttcac tttcagccat aaaactttgg aaaacttctt caaccatttc ccattcttcc
     2401 ggtgtttcta tagggaataa ttgtccagca tcatcataga ttgatgcaaa tacactaggt
     2461 tcttcaactg ttgtatcata atacaacaca tatcgttttc ctaattcctc attattaaaa
     2521 gtaaataata cttcaaattc tttttcatta ccttgatcat cgatcacttg gattttattt
     2581 gcttccattt aaattccttt ctattgggca tctaagtagc cttgtaaaat cgctactgca
     2641 gccattttgt caattacctg cttccgtttc tttcgagaaa catcagcaaa gataagttga
     2701 tcttgagcta ctttagttgt aagtcgctca tcaattaaaa caacattgag acccggtact
     2761 tctttttcta acatttcttt aaacatgatt gatatttgtg cacgctcacc caaatcacca
     2821 ttcatatgtt taggtagccc aagtacaacc gttttgatct gatgttcatt aattaaagca
     2881 acaacgtgct ttgcggctct atcataatca tcatctcgaa aacggtaagt ctcaattcca
     2941 tgagctaaca tgcctaaagt atcactgatt gcaataccac aggttcttga acctaagtcc
     3001 aaccctaata tccgttccat cctacttctc caaatatgat ttgactaatt cttcaataat
     3061 ttcatcacgt tcaatttgct ggattatatt tcgagcattt ttatgacttg aaatataagc
     3121 aggatcatta gagattaaat aacctgcaat ttgatgtact gcattatagc ctctttcatt
     3181 taatgcatca attactgttt taagattttg acgaatcatc tcacgacgca tatcttcaga
     3241 actgaatact tttgtctttg ttaagtcttg catattattc gacctccttt aaggctttgc
     3301 ctatagaatt attttacnnn nnnnnnnnnn nnnnnnnnnn naattactta gaaattaaaa
     3361 gaaattttta agttctccct tgatgttgga aatcttatca ttaattgggc agccaccttg
     3421 agcaaaatcc ggctttccac caccgcgacc atcaacaact gcattgattt ttttaataat
     3481 atcaccagct ttatatccag ttgcaacata atttttagtc accccgacaa agtatgaaca
     3541 tttgtcctca aattgtgtta ctaaaattac aattccttct tcaattttat ctctaaaatc
     3601 aaaggcaagt tgtttcgctt tagcagcatc aatcatttct tctacaaaca acactttaac
     3661 tccattgatt tc
//
LOCUS       DAOMTA010000222         3669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_885_length_3669_cov_2.184837, whole genome shotgun sequence.
ACCESSION   DAOMTA010000222 DAOMTA010000000
VERSION     DAOMTA010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_885_length_3669_cov_2.184837"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..448
                     /locus_tag="PLK68_07120"
     CDS             179..448
                     /locus_tag="PLK68_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRM90217.1"
                     /translation="MIQGKWIEDPTYCSIENFVDTFEPDGVITQKVYNKREITETYYG
                     TYVVKGNKLYITDSDETMIMTIKTLTTKQLVLLNDDGDILTYTKQ"
     gene            472..>806
                     /locus_tag="PLK68_07125"
     CDS             472..>806
                     /locus_tag="PLK68_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90218.1"
                     /translation="MKYIFLLLLMSIPINGIGQVDYSVVHVNEESGIKFTKITTDNDY
                     VCMPKVIRSGSTINWMSNRILDISVDGTQFAYLSCRNNTTNIFIKDIDKQGSSVQRTN
                     RQNVLDFSYS"
     assembly_gap    807..834
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <835..1440
                     /locus_tag="PLK68_07130"
     CDS             <835..1440
                     /locus_tag="PLK68_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90219.1"
                     /translation="ERNGTYNQIYQTDAKTGYICRQFTSNNKDFSPIYTFDMKNIFFS
                     RQEAKGISIWSYNIANNFLSNYTKGMNPFPLKSEEMIFCTRSNEKGHGEIWRINYITG
                     TEECIVSDPNRSFSSPSLSPDGRWILLVGSNILRNGAKMYANTDIFVCHNDGTQLTQI
                     TYHAADDLSPVWSRDGKYIYFISQRGSSKGIANVWRMSFKY"
     gene            1358..2155
                     /locus_tag="PLK68_07135"
     CDS             1358..2155
                     /locus_tag="PLK68_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRM90220.1"
                     /translation="MVNIYILFHKEVAQKASQMYGECHLNTNVQKYIILIKIIMRKIK
                     FIVAAMFLAVSSSAFAQFTNSSSQSSNVNADGWNTFYFQLNPSKISIDVKDSKNQSFT
                     GLTLGYGKAIHISSSEPLYFETGIAVQYSFYKDDDDDYETYDDHMASAKVPLNILYNF
                     QIPNSTIEIAPYLGATLRYNIWGEEKVTPNSKYKDEKEKTYNLFDKKDMGGSDYTWNR
                     FQIGWQVGVNARFNSKLLLGLSYGTDFSEIYKKGKINTTSITIGYCF"
     gene            2270..2725
                     /locus_tag="PLK68_07140"
     CDS             2270..2725
                     /locus_tag="PLK68_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90221.1"
                     /translation="MDNLFKNLLFVFALLCITSCSKNNNSNDAIDGDNKEQVISIIGA
                     WKLISDEGYVLDNGKKTIFENDESIGHIITFYSNGKITESGSDGGSGEWVKSGNTITV
                     KSNDTETGELLTRKINIISLSYSTLICDINGTESSNYSFYNKGIFERVE"
     gene            complement(2886..>3669)
                     /locus_tag="PLK68_07145"
     CDS             complement(2886..>3669)
                     /locus_tag="PLK68_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM90222.1"
                     /translation="LKLKLDLSKSEEHYRTSQLLLALSAFIILLLILAYRLIYKYNKN
                     RRIQIMKEAKKRILKLRMEELRNRVSPHFIFNVLNYEIYNREHGNKETNINCLIRLIR
                     TGLEQSDEISVPLYKELDFIDGYVELQRHSLSKEFVYNLEISDGVDDNTLIPSMIIQT
                     AVENAIKHGLRGLNGKTLLNIDIRKIKDILQIMVTDNGRGLIAPKSKDDSTGTGMTVI
                     RETLKILNEYNSKKIIYEMNINPIGKGCIVRIIIPLQYDYSL"
BASE COUNT         1294 a          495 c          591 g         1261 t
ORIGIN      
        1 attaactaat ttggattaca atgcggttgc attatacgct tgaattcagc tgttattaaa
       61 aaaactacta tttgttgtat taataggttt atgtatagtt tcgtgtagca aaagcgataa
      121 taatggactt attgatgaca agacagactc taatactgaa gaaaatacag tattaagtat
      181 gatacaaggt aagtggatag aggatcctac ttattgtagt attgagaatt ttgttgacac
      241 gtttgaacca gatggtgtta taacacaaaa ggtttataac aaaagagaga taacagaaac
      301 atactatgga acatatgtcg ttaaagggaa taaactatat attacagact ctgatgaaac
      361 aatgataatg acaattaaaa cactaaccac gaaacagcta gtccttctta atgacgatgg
      421 tgacatactt acatacacaa aacagtaatc ttgtcgtttt aaattaaaaa aatgaaatat
      481 atatttttat tattattaat gtcaatacca ataaatggta tcggacaagt agactattct
      541 gttgttcatg taaatgaaga atctggaatc aaatttacta agataactac agacaatgac
      601 tatgtatgca tgcctaaagt aatacgttct ggttcaacta taaattggat gtctaacagg
      661 atactggata tctctgtaga tggaacacaa tttgcatatt tgtcatgtag aaataataca
      721 acaaacatat ttattaaaga tatagataaa caagggagtt ctgtacaaag aactaatcgt
      781 caaaacgtat tagatttttc atattcnnnn nnnnnnnnnn nnnnnnnnnn nnnngaacgt
      841 aatggaacgt ataaccagat ctatcaaact gacgcaaaga caggatatat ttgtagacag
      901 tttacaagta ataataaaga tttttctccg atatatacct ttgatatgaa aaatatcttt
      961 tttagtcgac aagaagctaa aggtataagc atctggagtt ataatattgc taataatttc
     1021 ctttctaatt atactaaagg gatgaaccct tttcctttaa aatcagaaga gatgatattc
     1081 tgtacgcgta gtaatgaaaa agggcatggt gaaatttggc gaataaatta tattacgggt
     1141 actgaagaat gtatagtttc agatcctaat cgtagttttt cttcaccttc tctttctcct
     1201 gatggacgtt ggatattgct tgtaggtagt aatatcttaa gaaatggggc aaagatgtat
     1261 gcaaatacag atatatttgt ttgtcataat gatggaacac aattaacgca aattacatat
     1321 catgcagctg atgatctcag tccggtatgg agtcgtgatg gtaaatatat atattttatt
     1381 tcacaaagag gtagctcaaa aggcatcgca aatgtatgga gaatgtcatt taaatactaa
     1441 tgttcaaaaa tatattattt taataaaaat tattatgaga aaaatcaagt ttattgttgc
     1501 tgctatgttt ttagcagtaa gttctagtgc tttcgcacaa tttactaatt catcatctca
     1561 atcatctaat gttaatgcag atggatggaa cacattctat tttcaattaa acccaagtaa
     1621 aatttccata gatgtaaagg attctaaaaa tcaatccttt actggtttaa ctttaggtta
     1681 tggaaaagct attcatattt cttctagtga accattgtat tttgaaacag gaattgcggt
     1741 tcaatattct ttttacaaag atgatgatga cgattatgaa acatatgatg atcatatggc
     1801 atctgcaaaa gtaccattaa atatacttta taacttccag atccctaata gtactataga
     1861 aatagctcct tatttaggtg caactctccg ctataatatt tggggtgaag aaaaggtaac
     1921 accaaattct aaatacaaag atgagaaaga aaaaacatat aacttattcg ataagaaaga
     1981 tatgggtggt agcgattaca cctggaatag atttcaaatc ggctggcaag taggtgtaaa
     2041 tgctcgcttt aacagtaagt tgttacttgg attatcttat ggaacagatt tttccgaaat
     2101 atataagaaa gggaaaatta atacgactag tataacaatt ggatattgtt tttaaattaa
     2161 tgaaggctgg aaaatagtta ataatttttc gtttttaatt attgctaagt aggactgctc
     2221 gccattgaat aattttaatt gatggcgagc ttaataaaat ttcaataata tggataatct
     2281 atttaaaaat ttattgttcg tttttgcact tttatgtatt acatcatgta gtaaaaataa
     2341 taatagtaat gatgctattg atggagataa taaagaacaa gttatttcaa ttatcggggc
     2401 ttggaaatta ataagtgacg aaggatacgt attggacaat ggtaaaaaaa cgatatttga
     2461 gaatgatgaa tcaatcggtc atattataac attttatagt aatggaaaaa taacagaaag
     2521 cggaagcgat ggtggttctg gagaatgggt taaatctggt aatacaataa cagttaaatc
     2581 taacgataca gaaacaggag aacttctaac tagaaaaatt aacataattt ctttaagtta
     2641 ttcaacgctt atttgtgaca ttaatggtac agagagcagc aattattctt tttacaataa
     2701 aggtatattt gaaagagttg aataaattgt ttttttaatg gctagctgct tgattcttaa
     2761 ttgacaatat aagttgcttt aagagtgtaa agtaaactgt gccaagttcc aataatagtc
     2821 taacccattg gcagaattaa atgagcgtat atttaatttc accaacagat ttacattctc
     2881 ttttcctata aagaataatc gtattgcaat ggtatgatta ttcttactat acagcctttt
     2941 ccaattggat taatattcat ttcatatatt attttctttg aattatactc atttagtatt
     3001 tttagagttt cacgtatgac agtcatacct gtaccagtac tatcatcttt tgatttaggc
     3061 gcgattaaac cacgaccatt atcagtaacc ataatttgta gtatgtcctt tattttacgt
     3121 atatctatat taaggagagt cttgccattt aatcccctta aaccatgctt tattgcattt
     3181 tctacagctg tttgtataat catggatggt attaatgtat tatcgtcaac accatcacta
     3241 atttctagat tatatacaaa ctcctttgat agactatgac gttgtagttc tacatatcca
     3301 tcaatgaagt ctaattcctt ataaagagga acacttattt catctgattg ctctagtcca
     3361 gtccttatta agcgtattaa acagtttata ttagtttctt tatttccatg ttctcgatta
     3421 tatatctcat agttcaatac attaaaaata aaatgtggcg ataccctgtt ccttaattcc
     3481 tccatacgca gttttaatat tctctttttt gcttctttca ttatttggat acggcggttc
     3541 ttgttatact tgtatattaa ccgatatgct aatataagta gtaatattat aaatgcagat
     3601 aaagcaagta atagctgaga tgtacggtaa tgttcttcac tctttgatag atctaattta
     3661 agttttaag
//
LOCUS       DAOMTA010000223         3667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_886_length_3667_cov_2.285714, whole genome shotgun sequence.
ACCESSION   DAOMTA010000223 DAOMTA010000000
VERSION     DAOMTA010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_886_length_3667_cov_2.285714"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PLK68_07150"
     CDS             complement(<1..1289)
                     /locus_tag="PLK68_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM90223.1"
                     /translation="MMKSIYGKLICGFLITIAFSFSVAGYVALRNNYDQIEDMAKSEL
                     SATSEYVVGILNSLDDANINDIMNKYAISSEVYITLYSPELDYYTYGENKGYFPSRET
                     MIQYYHDENKNGRFDERKSVQTYGSKAKINGSDVYIFIQKDTSYEKGIFANSAVLILG
                     CVFLSGNLVFLAIADIIVKPITRLTNATKELSKGNYSVRVNYVGDDEISRLNQGFNQM
                     AQQLAKQEETRQKFISDISHEFQTPLTAIQGFANILKEEDLPKEQRQKYADIILFHSK
                     RLSTLSKNMLQLTLLEREEVELEFTTYSIVEQLSRVISTQENQAILKDIEIQFEKPRK
                     DIMVYGDEQRLEQVWINLISNAIKYTGEGGLITVTVKKASREVEVSIEDTGYGMSKEV
                     VSHIFERFYREEKARSVEGNGLGLSIVKTIVDLHHGN"
     gene            complement(1286..1957)
                     /locus_tag="PLK68_07155"
     CDS             complement(1286..1957)
                     /locus_tag="PLK68_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM90224.1"
                     /translation="MKAKVLVVDDEKDIRELINFYLNKEGFEVLEASNGEEALEIFEN
                     EYIDLGIIDVMMPVMDGFELVENLKEFKDVPVIMLTAKGESKDKLRGFSVGADDYVVK
                     PFDPTELMARVRAVLKRYSVNTSNIIKLNEVEFDGDKYEIRYQSEIIHLPLKQFELVF
                     ELAKHPDQIFSREQLIEKIWGMDYDGFDRTVDVHIKRIRENLGHLPGFKVVTVRGLGY
                     KVEVE"
     gene            complement(2110..>3667)
                     /locus_tag="PLK68_07160"
     CDS             complement(2110..>3667)
                     /locus_tag="PLK68_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRM90225.1"
                     /translation="TITLIIGLIGEYAFLIPLNLRSPQFIIYFCILLLIFNGLYFMLS
                     QRFSKITKYSLIVIGILIAYVAIGTFVSSPIFNAGSYQKQLKLDKKADFYADNKTISY
                     QSIPVVDRDSAIKLGDRKMGQMVDYVSQFEVDESYEQINYQDTPYRVTPLEYSDLIKW
                     FTNRSDGLPAYIRVNMVTQESEVVKLKEGMKYSKSEHFGRKIERHLRANYPTLMFDTL
                     AFEIDEKGNPYWIAPVYNYKIGLFGGKDITGAVLVNAINGDHEYYDIDKVPEWVDRAY
                     PAELVLSQLENYGKYTNGYFNTLFSQKGVLQPTSGYNYVAINDDVYLYTGLTSVSNDA
                     SNVGFAMINLRTKDGKYYNISGAEEYSAMSSAEGQVQNLKYTATFPILINAGGQPTYF
                     LSLKDDAKLVKKYAFVSVENYQIVATGDSVAQAEQAYYALLEANGKKTDSSEYKTNEL
                     TGAITAINEAVVDGNSTYYFKLEGSDTIFIGDISLSNQFPLAKVGDVVTIEYVNSKDN
                     SEVITSIKFG"
BASE COUNT         1222 a          725 c          449 g         1271 t
ORIGIN      
        1 atatttccat gatgtaaatc tacaattgtt tttacaattg ataaccctag tccatttcct
       61 tctacagaac gggctttttc ttcacgataa aaacgttcaa atatatgtga aacaacttct
      121 ttagacattc cgtatccagt atcttcaatt gatacttcaa cttcacgaga agccttttta
      181 acagttactg taataagacc accttcacca gtatatttga ttgcatttga aataagattg
      241 atccaaactt gttccaatcg ttgttcatcg ccataaacca taatatcttt gcgcggtttt
      301 tcaaattgaa tttctatatc ctttaaaatt gcttggtttt cttgagtaga aataacccga
      361 gataattgct caacaattga atatgttgta aattctaact caacctcttc tcgttccaac
      421 aacgtcagct gcagcatatt cttagaaaga gtcgataacc tttttgaatg aaataagatt
      481 atatcagcat acttttgacg ctgctcttta ggaagatctt cctcttttaa aatattggca
      541 aagccttgaa ttgcagtcaa tggagtttga aattcgtgcg aaatatcaga aatgaacttt
      601 tgtcttgttt cttcttgttt cgctaactgc tgtgccattt gattaaatcc ctgattcaat
      661 cgagaaattt catcatcacc cacataatta acacgaacac tataatttcc ttttgataat
      721 tctttagtgg cgttagttaa tctcgttatt ggtttaacaa taatatcagc aatcgctaaa
      781 aaaactagat ttcctgataa aaagacacaa cccaaaatca aaacagcaga attagcaaaa
      841 attccttttt cataactagt atccttttga ataaatatat atacatcaga accattgatt
      901 tttgccttgc tgccataggt ttgcacactt ttgcgctcat cgaaacgccc gtttttgttt
      961 tcatcatgat aatattgaat cattgtctcg cgactaggaa aatatccttt gttttcacca
     1021 tatgtataat agtccaattc tggtgaatac aacgttatat aaacctctga gctaattgcg
     1081 tatttattca taatatcgtt aatattagca tcatcaaggc tatttaaaat acctacaaca
     1141 tattcacttg ttgcactcaa ctcactctta gccatatctt caatttgatc ataattatta
     1201 cgtaaagcta catatccagc aaccgaaaaa ctaaatgcga tagttattaa aaaaccgcaa
     1261 atcagcttgc cataaataga tttcatcatt cgacttcaac cttgtatcca agtcctctca
     1321 ccgttacaac tttaaatcct ggtaagtgtc ctagattttc tctaattcgc ttaatgtgaa
     1381 catctacagt acgatcaaag ccatcatagt ccattcccca aattttttca atcaactgtt
     1441 cacgactaaa aatctgatct ggatgctttg ctaactcaaa aactaattca aattgtttta
     1501 acggtaaatg aatgatttcg ctctgataac gtatctcata tttatcgcca tcaaactcaa
     1561 cttcatttaa tttgattata tttgatgtat taacactata tctttttaaa actgctcgca
     1621 ctcgtgccat taattcagtt ggatcaaatg gcttaactac atagtcatca gcaccaacag
     1681 aaaaaccgcg aagtttatct ttactttcac ctttcgctgt taacataatt actggaacat
     1741 ctttaaattc ctttagattt tctactagtt caaaaccatc cataactggc atcatgacat
     1801 caatgattcc taaatcaata tattcatttt cgaatatttc taaagcctct tcaccattgc
     1861 tagcttccag aacttcaaat ccttctttat tcaaataaaa attaattaat tccctaatat
     1921 ctttttcatc atcaactact agtacttttg ctttcatctt tacacctcaa ttcactgtaa
     1981 gattataaca cattttatta actaaatata tagttacaaa agcaaaatag cttgagttta
     2041 aagagatgat aataaatata aaaaagccat aacctatata taagttatag cttcttgttc
     2101 tattaaagac taaccaaatt taattgaagt aattacctcg ctattgtctt tactattaac
     2161 atattcaatg gttacaacat caccaacttt tgctaatggg aattgattag ataaactgat
     2221 atctccgatg aaaatcgtat cactaccttc aagtttaaag taataagtac tgttaccatc
     2281 aacgacagct tcattaattg cagtaatcgc tccagtcaac tcattagtct tatattcact
     2341 tgagtcagtt tttttaccat tagcttctaa taaagcataa tatgcttgtt cagcttgagc
     2401 aacactgtca ccggtagcca cgatttgata attttcaaca ctaacaaaag catacttttt
     2461 aacaagttta gcatcatctt ttaaagataa gaaatatgtt ggctgccctc ccgcattgat
     2521 caaaatcgga aaagttgccg tatatttcaa gttttgaact tgaccttccg ccgaagacat
     2581 tgcagaatat tcttcagctc cagaaatatt ataatacttt ccatctttag tccgcaaatt
     2641 aatcatagca aacccaacgt tagaagcatc attagatacc gatgttaaac cagtatataa
     2701 atagacatca tcattgattg ctacataatt gtaaccagag gttggctgta atactccttt
     2761 ttgcgaaaat aaagtattaa aatacccatt tgtatattta ccataatttt ctaattgact
     2821 taaaaccagt tctgctggat aagcacggtc aacccattct ggtactttat caatatcata
     2881 atattcatga tcaccattta ttgcattaac taatacagct ccagttatat cttttccacc
     2941 aaataaaccg attttataat tatatactgg tgcaatccaa taaggattcc ccttttcatc
     3001 aatttcaaaa gccaatgtat caaacattaa agttggataa ttagcgcgca aatgacgttc
     3061 aattttacgt ccaaaatgtt ctgatttaga atatttcatt ccttctttta atttaactac
     3121 ttcagattct tgcgtcacca tatttactcg gatatatgcg ggcagtccat cactacggtt
     3181 agtaaaccat ttgatcaagt cactatattc taatggtgtc acacgatatg gcgtatcttg
     3241 ataatttatt tgttcatacg actcatcaac ctcaaattgg gatacataat caaccatctg
     3301 tcccatttta cgatcaccta atttaatcgc actatctcga tccaccactg gaatcgattg
     3361 ataggaaata gttttattgt cagcatagaa gtctgctttc ttatctaact ttaattgttt
     3421 ttgataactt cctgcattaa agataggcga actaacaaat gttccaatgg ccacatatgc
     3481 aattaatatt ccaatcacaa tgagactata tttggtgatc ttactaaatc tttgacttaa
     3541 cataaaatat agtccattaa atattaataa taatatacaa aaataaatta taaattgtgg
     3601 tgaacgaaga ttcaatggga tcaaaaaagc atattcacca atcaatccaa ttattaaagt
     3661 tattgta
//
LOCUS       DAOMTA010000224         3658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_887_length_3658_cov_2.470996, whole genome shotgun sequence.
ACCESSION   DAOMTA010000224 DAOMTA010000000
VERSION     DAOMTA010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_887_length_3658_cov_2.470996"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLK68_07165"
     CDS             <1..912
                     /locus_tag="PLK68_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HRM90226.1"
                     /translation="GVTIFGQIFKNSNDQRDYQELSTLQLFDSNSSITQEGNLKNGGY
                     AFLTDSNIYFGMDYQIYQTERNFSNFKSLVDDGERYIYSTDDYLYYEQYGRYARYDMK
                     SGELTALFEMDNVLPIDKNKFLYTKYDEEGLFIYDEVSATSIKIISDEISDYSYDMQD
                     SLVFYTTIEHDYIQAIDLAGNKLSQFNLSSTGKIYVSGDLLYYQDYQGVHCYSIADEN
                     DELLVEGEVNNYIVTNNTIVYTNYDDDLMTSDGHIVSIDYDVTVFNVIGNYIVYSTGN
                     GDEYLKQWYINDFYDTAIAKLNNNEEE"
     gene            1013..2248
                     /locus_tag="PLK68_07170"
     CDS             1013..2248
                     /locus_tag="PLK68_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HRM90227.1"
                     /translation="MEKIKRCRKCGARLLEHEKRCPVCGTPVGLDKEVDIIKEEQIIE
                     PEKVDEFIAADKTEQTLLKSNESAQNYWRSKKIWAVFIVLIVVTTVMRQYVINNPIKL
                     AENDNSNTITNDYSDSKISINKNTGKYSQATNINYLGISYVNDDAVYLMMNSELLKYD
                     RSFNNRELVLEQAVTVFSEDEQWYYYLDENNDYIRMDKKTKAEDILLKNVYYVHNLGD
                     KVYYQNDSDGETIHCLELETNQDHKISDEVSYSIVVDEEKGRIFYINKNNELVSIALD
                     GSDKKNLANNTNVYTYDGEYLYYINNDGLVKSDLEGQSKVIYESNNLSLVNLVEKKLV
                     IQDKNIIYTMDLDGKDKKKLYTMDIGGSLTFEVVGDKLLVLTKGNSDSVIGYEIVGLD
                     GKRHILDDENQPTIKGNEF"
     gene            2258..3610
                     /gene="murF"
                     /locus_tag="PLK68_07175"
     CDS             2258..3610
                     /gene="murF"
                     /locus_tag="PLK68_07175"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRM90228.1"
                     /translation="MKVSEIVIATGGKLISGDETMEITGFSQDSRQAEVGMMYIPIIG
                     ERYDGHDFIESAFTNGASAIITDRLIEDDKHVVILVDNTLKALQKMAHYLRKHRKVKV
                     VGITGSVGKTSTRDMVYSVVKQQYKTLKTEGNYNNNIGLPLTILRLKDEEVMILEMGM
                     NHLNEMEELSRIACPDISAITNVGTAHIGELGSRENILKAKLEIVAGMSDGSTLVING
                     DNDMLSTVRFDNFKVVKVGVDCAAVFKAEKVILMDDHSEFTINYNGKVYQVVVPVPGN
                     HFVLNALVAIAIGINLEIPMEKCIQGISQFELTKKRMDVIELKNNITLIDGTYNASED
                     SMKSSIDVLATYSRRKIAVLADMLELGEFSEQLHRSVGKYVAEKQIDVLVAVGREAKF
                     MSDSAALSGMAEIYYCNNNQEVVNYLKNNLQNDDVVLLKGSNGMKLKDVVTKIEEKFS
                     "
BASE COUNT         1324 a          355 c          790 g         1189 t
ORIGIN      
        1 ggagtaacta ttttcgggca aatttttaaa aattcgaatg atcaacggga ttatcaagaa
       61 ttatcaacat tacaattatt tgatagtaat agcagtataa cgcaagaggg aaatttaaaa
      121 aatggtggtt atgctttttt aacggattca aatatttatt ttgggatgga ttatcaaata
      181 tatcaaacag aacggaattt tagtaacttt aaatcgcttg tagatgacgg agaaaggtat
      241 atttatagta ctgatgacta tctttactat gaacagtatg gaagatatgc acgttatgat
      301 atgaaaagtg gtgaattgac agcccttttt gaaatggata atgtattacc aatagataaa
      361 aataagttcc tatatactaa gtatgatgaa gagggactgt ttatatatga tgaggtaagt
      421 gcaacatcaa taaaaatcat aagtgatgaa atttcagatt atagttatga catgcaagat
      481 agtttagttt tttatacaac aattgaacat gattatattc aggctattga tttggccgga
      541 aataaattat cacaatttaa tttatctagt actgggaaaa tttatgtgag tggagatttg
      601 ctttattatc aagattatca aggcgtacat tgttatagta ttgctgatga aaatgatgaa
      661 ctgcttgttg aaggtgaggt taataattat atcgttacaa ataatacaat tgtatatact
      721 aactatgatg atgatttaat gactagtgat gggcatattg tatcgattga ttatgatgta
      781 actgtattta atgtaatagg caattatatt gtgtatagta caggaaatgg tgatgagtat
      841 ttaaagcagt ggtatataaa tgatttttat gatacggcta tcgctaaatt gaataataat
      901 gaagaagaat agactaagct tattgaagcg aagtcttttc ttttacgcta ataacacgct
      961 tttttttact ttggtgatat ggtaaaatgt aggcattaaa gtaggtgact aaatggaaaa
     1021 gattaaaaga tgtagaaaat gtggagcgcg attattagaa catgaaaaaa gatgtcctgt
     1081 ttgcggaacg ccggtagggt tagataaaga agtagatatt ataaaggaag aacagattat
     1141 tgaacctgaa aaagtcgatg agtttattgc agctgataag actgaacaaa cattgcttaa
     1201 gtctaatgaa tcagcgcaaa attattggcg tagtaagaaa atttgggctg tttttattgt
     1261 tttaattgta gtgacgacag taatgcgtca atatgttatt aataatccga ttaagctggc
     1321 agagaatgat aattcaaaca caatcactaa tgattatagt gatagtaaaa tatcaatcaa
     1381 taaaaataca ggtaaatata gtcaagcgac aaatattaat tatttaggga ttagttatgt
     1441 taatgatgat gctgtttatt taatgatgaa ttctgaatta ttgaaatatg atcgtagttt
     1501 taataatcgc gaacttgttt tggaacaagc agttactgtt tttagtgaag atgagcagtg
     1561 gtattattat cttgatgaaa ataatgatta tattcgaatg gataaaaaga caaaagcaga
     1621 agatatttta ttaaagaatg tatattatgt acataatctt ggtgataaag tctattatca
     1681 aaatgatagt gatggtgaaa caattcattg tcttgaatta gagacaaatc aggatcataa
     1741 gattagtgat gaggtttctt atagtattgt tgttgatgag gaaaaaggta gaatttttta
     1801 tattaataaa aataatgaat tagtttcgat tgcattggat ggcagcgaca agaagaatct
     1861 tgccaataat actaatgttt atacttatga tggtgaatat ttatattata taaataatga
     1921 tgggctagtt aaaagtgatt tagaaggaca aagtaaagtt atttatgaaa gtaataattt
     1981 atcattagtg aacctggttg agaagaaatt agttattcaa gataagaata ttatttatac
     2041 gatggatctt gatggaaaag ataaaaagaa gttatatacg atggacattg gaggcagtct
     2101 gacatttgag gttgtaggtg ataagctgct ggtgttaact aaaggaaact cagatagtgt
     2161 gattggatat gaaattgttg gtcttgatgg gaagcgccat attttagatg atgaaaatca
     2221 accaacgatt aaaggtaatg agttctaggg ggatgaaatg aaagtaagtg agatagtaat
     2281 tgctaccggt gggaaactta ttagtggtga tgaaacaatg gaaataacag gttttagcca
     2341 agatagtcgt caagctgagg tcgggatgat gtatattcca attattggtg aacgctatga
     2401 tggccatgat tttattgaaa gtgcatttac aaatggtgcc agcgctataa ttactgatcg
     2461 gttgattgaa gatgataagc atgttgttat cttagtagat aatacgttaa aagctttgca
     2521 aaagatggct cattatttac ggaaacatcg caaggtcaaa gttgttggaa ttactgggag
     2581 cgtgggtaaa acaagcacac gtgatatggt ttatagtgtt gttaaacagc agtataagac
     2641 attgaaaaca gaaggtaatt ataataataa tattggcttg ccattgacaa tcttgagatt
     2701 aaaagatgag gaagtgatga ttttagaaat gggtatgaat catcttaatg aaatggaaga
     2761 attatcacga attgcttgtc cggatattag cgcgattact aatgttggaa cagcacatat
     2821 tggagaatta ggaagtcgtg aaaatattct aaaagcaaaa ttagaaattg ttgcgggaat
     2881 gagtgacggc agtacattag taattaatgg tgataatgat atgctgtcaa cggttcgatt
     2941 tgataatttc aaggttgtta aagtcggtgt tgattgcgct gctgttttta aagctgaaaa
     3001 agttatttta atggatgatc atagtgaatt tacaatcaat tataatggga aggtctatca
     3061 agttgttgtc ccggtaccgg ggaatcattt tgttttaaat gctcttgttg cgattgcgat
     3121 tggaataaat ctagaaattc caatggaaaa atgtattcaa ggaattagtc aatttgaatt
     3181 aactaaaaaa cggatggatg taattgaatt aaaaaacaac attaccttga ttgatggcac
     3241 atataatgct agtgaagatt caatgaaatc aagtattgac gtattggcta cgtattcgcg
     3301 tagaaaaata gctgtattag cggatatgtt agaattgggc gaattcagcg agcaattgca
     3361 tcgaagtgtt ggtaaatatg tagcggaaaa acaaattgat gtgttagttg cggttggccg
     3421 agaagctaag tttatgagcg atagtgctgc tttgtcaggg atggcagaga tttattattg
     3481 taataataat caagaagtgg taaattattt aaagaataat ttacaaaatg atgacgttgt
     3541 cttattgaag ggctcaaatg gaatgaaatt aaaagacgtt gtcaccaaaa tagaggagaa
     3601 attttcatga aacaaaaatt attggtttta tgtgggggac aatcaagtga gcatattg
//
LOCUS       DAOMTA010000225         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_896_length_3627_cov_2.440929, whole genome shotgun sequence.
ACCESSION   DAOMTA010000225 DAOMTA010000000
VERSION     DAOMTA010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_3627_cov_2.440929"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="nadA"
                     /locus_tag="PLK68_07180"
     CDS             complement(<1..718)
                     /gene="nadA"
                     /locus_tag="PLK68_07180"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRM90229.1"
                     /translation="MIKNEWIEKGYIDEPIDKNIEIVKEIRQMCKDKNAIILAHYYTQ
                     GAIQDIADFIGDSLALARKAENTDAKIILMCGVNFMAETCKILNPSKLVLAPDLNSGC
                     SLADSCKAEDLKKYKEEHPGYKVVSYVNTTAAVKALTDIVVTSGNAKKIVDTFPKDEK
                     LIFGPDYNLGSYINQITGRNMLLWNGGCHVHERFSVDEIIKLKKLHPNAKVLAHPECK
                     GPVLAVADVIGSTAVLLNYSI"
     assembly_gap    920..929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1250..3307
                     /locus_tag="PLK68_07185"
     CDS             1250..3307
                     /locus_tag="PLK68_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM90230.1"
                     /translation="MKSLKLSISTFLKISGLIVVLGFILRVVLLFNPSTVVNYSFLEW
                     IQIFLLGTFNDIFFAVISFSFFCLFLLFFSEVKYKKPWSYIIYALLVITYIVLQFINT
                     PLREFNSSLTRIICYLLLYRIASYTLRLFFPVIREYWRQYTYYIVFFVYVLFIVFNAI
                     AEYFFWSEFGLRYNFIAVDYLVYTNEVIGNFFESYPMVPLISLVVLLSGGISYGLLRN
                     IRKNNFNIIPSLRDKLIATSAYIVLSSASFGLLFVNFSFQHGNNSYVNELQANGCVKF
                     CQAFFSNKLCYKDFYNKISDAEAVGIINSQYGRSIDNIQHIHSDKPEIHKNIVLITIE
                     SMSGEFLKRFGNTQNITPNLDNLMQQGLCFDNLYAVGNRTVRGLEAVTLCIPPSSGES
                     IIKQENNSGLFSTAKVLHSKGYNVNFIYGGDSYFDNMKSFFSGNGFKVIDKSDFKGKE
                     LTFSTVWGACDEDMFNKAIKVFNGNAKVKKPFFANIMTVSNHRPYTYPDGRIDIPSSN
                     KCREGGVKYTDYAIGKFFEMAKKQPWFNNTIFIITADHCASSAGSTDIPLDQYHIPAL
                     IYAPGFVKPSVNKTLLSQIDLMPTVFGLLHFSYESRFFGQNVLSKSYHPRAFAATYQN
                     LGYIENNKMIVLSAGHRQQQFRLLSGKYSLTTGAEQHNIDKSILHHAIANYQCAGDIQ
                     KKK"
     gene            complement(3324..>3627)
                     /locus_tag="PLK68_07190"
     CDS             complement(3324..>3627)
                     /locus_tag="PLK68_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90231.1"
                     /translation="HIAGKKENGDIIFYRPPLNERVSAGLSIRGEFVMPIFSINLGLG
                     HNFIYKGKNFSGLYQVAVLKTSITRNLFLHIGYQLSEFKNPRNLMIGFGYHFHNKR"
BASE COUNT         1185 a          533 c          542 g         1357 t
ORIGIN      
        1 ttattgaata attaagtaat accgcagtag aaccaataac gtcagctaca gccaatacag
       61 gacctttaca ttcaggatga gccaatacct tagcattggg atgtaatttt tttaatttaa
      121 tgatttcatc tacagaaaat ctttcatgta catgacaacc tccattccat aataacatat
      181 tacgacctgt tatctgattt atataactac ctaagttata atcaggacca aatataagtt
      241 tttcatcttt aggaaatgta tcgacaatct ttttagcatt acccgaagtt actacgatat
      301 cagtaagtgc ctttacggct gcagtagtat ttacataact tacaacttta tatccaggat
      361 gttcttcctt gtattttttc aaatcttcag ccttgcaact gtcagcaaga gaacaacctg
      421 aattaagatc aggagccaat accaattttg aaggattgag tattttacat gtctctgcca
      481 taaagttaac tccacacatc aaaataatct ttgcatcagt attttcagcc tttctggcaa
      541 gagcaagtga atctccaata aaatcagcaa tatcctgtat agcaccttgt gtgtaatagt
      601 gagcaagaat tatagcattc ttgtctttac acatttggcg tatctctttt actatctcta
      661 tatttttatc tataggttca tctatataac ctttttcaat ccattcattt ttaatcataa
      721 taatattata atatatattt attctttgta tttaaataaa atgtaaaact atgatgtact
      781 gttgatacgt tgataacttt tatttaaaaa tcttgtttta taagatataa acataagaaa
      841 cctgtttatc cacattagta tttaattatt actcattgat aatgagctaa aaacaatact
      901 tatcaacaat tttatatttn nnnnnnnnng gtttatcaac atttccccaa ttcggtgcaa
      961 agttaataag tttatatctt tttctatgta aaaatcgtgg aaaacttata aaaaacttaa
     1021 taataactat gtggatatcc actaaaatag aatacactgt ttggtcttgt ttattatcca
     1081 caagttatta ttttgttatc cacattgtta tccacacact tatcaacatt aaaatatttt
     1141 gttatggcta tgaactttat atatagaata tactatatta attgatatat tatgtgatta
     1201 tttaaaagat aaacattata tttgctgact tttttaaaat aataataata tgaaaagttt
     1261 aaaattgagt atatctacat ttcttaagat atcaggtctt atagttgtat tgggctttat
     1321 attgagggtc gtattactat ttaatccttc tacagtagta aattattcat ttttagaatg
     1381 gatacaaata tttttacttg gtacattcaa tgatatattc tttgctgtaa ttagcttttc
     1441 atttttctgt cttttcttat tatttttttc ggaagtgaaa tataagaagc catggtcata
     1501 tattatttat gcattactgg ttattacata tattgtactt caatttatta atacaccgtt
     1561 acgcgaattt aattcatcac ttacacgaat aatatgttac cttttattat atcgaattgc
     1621 tagttataca ttacgtttat tttttcctgt cattcgtgaa tactggcgtc agtatacata
     1681 ttatatagta ttctttgttt atgttttgtt tattgtattt aatgctattg ccgaatattt
     1741 tttctggagt gaatttggat tacgttataa ttttatagct gtagactatt tggtatacac
     1801 aaatgaagta attggtaatt ttttcgaatc ttatcccatg gttccattaa tatcgttagt
     1861 tgtattatta tctggtggca tatcatatgg tttacttcgt aatataagaa aaaataattt
     1921 taatataatc ccttcattac gtgataagtt gattgcgact tctgcatata tagttttgtc
     1981 ttctgcatct tttggacttt tgtttgttaa tttttcattt caacatggca ataatagtta
     2041 tgtaaatgaa ctacaagcta atggttgtgt taaattctgt caggcatttt tcagtaacaa
     2101 attatgttat aaggactttt ataataaaat atcagatgca gaagctgttg gcatcattaa
     2161 ttcacagtat ggtagaagta tagataatat tcagcatata cattctgata agccggaaat
     2221 acataagaat attgtattga ttaccattga aagtatgagt ggagagtttt taaagcgttt
     2281 tggaaataca cagaatatta caccgaatct ggataatctt atgcaacaag gcttgtgttt
     2341 tgacaatctt tatgccgtag gaaaccgtac tgtacgcgga cttgaagctg ttacattatg
     2401 tatacctcca agttctggtg aaagtattat taaacaagaa aataatagtg gcttgttttc
     2461 tacagctaaa gtattgcatt ctaagggtta taatgtaaat tttatatatg gtggcgacag
     2521 ttattttgat aatatgaaat ctttctttag tggaaatgga tttaaagtaa tagataaatc
     2581 tgattttaaa ggaaaagagt tgactttcag tacagtatgg ggagcttgtg atgaagatat
     2641 gtttaacaaa gcaataaagg tattcaatgg caatgcaaaa gttaaaaaac cattttttgc
     2701 caatattatg actgtaagca atcaccgtcc atatacttat ccagatggta gaatagatat
     2761 accttcatca aacaaatgtc gtgaaggtgg cgtaaaatat actgattatg ctattggtaa
     2821 attctttgaa atggctaaaa agcaaccttg gtttaataat actattttta taattactgc
     2881 cgatcattgt gcttcgagtg caggaagtac tgatattcct cttgatcaat atcatattcc
     2941 tgctttaata tatgctcccg gattcgtaaa accgtcagta aataaaacgt tgttatctca
     3001 gatagatttg atgcctacag tatttggact tttgcatttc agttatgagt ctcgtttctt
     3061 tggtcagaat gtgttgtcaa agtcgtatca tcctcgtgct tttgccgcta catatcaaaa
     3121 tttaggatat atagaaaata ataaaatgat agtattatcc gccggccatc gtcagcaaca
     3181 gttcagactg ttgtctggta agtattcact tactaccggt gctgagcaac ataatataga
     3241 taaaagtata cttcaccatg ctattgctaa ttatcagtgt gcaggtgata tacaaaagaa
     3301 aaaataaatg cttacctatt ttattatctc ttgttatgga aatggtagcc gaaacctatc
     3361 ataagatttc ttggattttt aaattcactc aactgatatc cgatgtgtag aaataggtta
     3421 cgtgtgattg atgtcttcag aacagcaact tggtataaac cactgaaatt tttgcctttg
     3481 taaatgaaat tatgacctaa accaagatta atagaaaata ttggcattac aaactctcca
     3541 cgaatagaga gtccggctga tactctttca ttcaaaggag ggcgataaaa gattatatct
     3601 ccattctctt tttttccagc tatatgg
//
LOCUS       DAOMTA010000226         3622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_900_length_3622_cov_2.735913, whole genome shotgun sequence.
ACCESSION   DAOMTA010000226 DAOMTA010000000
VERSION     DAOMTA010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_900_length_3622_cov_2.735913"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..1182)
                     /locus_tag="PLK68_07195"
     CDS             complement(373..1182)
                     /locus_tag="PLK68_07195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM90232.1"
                     /translation="MKKLLSIFLIMLCFSGCKQNNDSELIKTEAVEAQQTAFVEKEGE
                     VLVPESLRTVQLPIVGDSRVIVIDAGHQRRGDSNKEPIGPGASQTKAKVTTGATGIST
                     GNLESAINLEVALKLQKKLEDSGYQVIMVRTSQDVNISNQQRAEIANKNNAGAFIRLH
                     CNSDDSSSIHGTLTMAPSESNPYCSQIATASQRLSKTVVNSICNQTGSKNRGVIITDV
                     MSGINWCQVPVTIVEMGFMSNPDEDRLLGDSTYQDKIVTGIVLGINEYFKN"
     gene            complement(1216..2181)
                     /locus_tag="PLK68_07200"
     CDS             complement(1216..2181)
                     /locus_tag="PLK68_07200"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM90233.1"
                     /translation="MRQDDDLKKVVLVGTGFVGMSMAYSILNTGGIDELVLIDVDQEK
                     AIGEAMDISHGLPYSKSSLKVKAGGYDECKDADIVVITAGAAQKPNQTRLELASVNAK
                     IMKSITEQIMASGFDGIIIVASNPVDLMSYVVQKVSGLPTSRVIGSGTILDTARLRYL
                     LSEYLNISSTNIHAYILGEHGDSSFVPWMNTYIGCKSMMEYIVEMNIDMNEMHKIYKE
                     VQQAAYEIIKRKNATYYGIGLSLNRLITAILSDENAVLTVSAYQQGEYKQEGLYIGVP
                     AIINRQGISKIMTLHLNNVDQHKFDRSCETLKEMIDGELEAIINS"
     gene            complement(2487..3308)
                     /locus_tag="PLK68_07205"
     CDS             complement(2487..3308)
                     /locus_tag="PLK68_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HRM90234.1"
                     /translation="MEILLEPIQESLMTVPVLFLACLLVEYISNRDVVNKVMEFGKVG
                     PFIGAILGSIPQCGFSVIAARLYSMRYLTMGTLLSIFIATSDEALAILVVHPDLWEML
                     VVLIVLKIVLGTVTGYVVDRLNHRSHDDYEYLQVEPCDCGCQNGIWLPALRHTLNIFI
                     FILLTNIVFTLIIAGIGEETVTNLLKTNWIFQPIIAGVIGFIPNCAGSVILTQLYVSG
                     GLSFGALLTGLTTSAGVGTLALIKYNENKKDTFKILFISYVIALVVGYIASLVML"
BASE COUNT         1250 a          686 c          417 g         1269 t
ORIGIN      
        1 catgtctaca cctcacatta ctacatttta gcataaaaaa ttaataactt ttagtaaaac
       61 ttaaaaaaca ttataaaata ttatgtgaca aattttaatc atcccctgct gctcaacaat
      121 attattagca ctatcaagca cggaattatt gtataggaaa agcaaaatat ctagttcact
      181 gcaaatgatt tgatactgtt taattacatt ttgatataaa taatcataag ttttttacat
      241 catttagaat tcttaataac tttatcatac attctccaat tagttctatt ttaaactata
      301 ttttattact atattaaaaa aatattatac aaaaaagctg aaagtttcgg aactttccta
      361 atactttcaa ctctagtttt taaaatattc atttattcct aaaacaattc ccgtaacaat
      421 tttatcttga taagtactgt ctcctaataa cctatcctca tctggattag acataaatcc
      481 catttctact attgttacag gtacttgaca ccagtttatt ccactcatca catcagtaat
      541 aataacgcca cgatttttgc tgcctgtttg attacaaata ctatttacaa ccgtttttga
      601 caatcgttga gaagctgtag caatttgact acaatatgga ttagattccg atggtgccat
      661 agttaaagtt ccatgaatac tactactgtc atcactatta cagtgcagtc gaataaatgc
      721 tccagcatta tttttattag caatctccgc acgctgttga ttagaaatat tcacatcttg
      781 acttgtccga accataatta cttgataacc cgaatcttct aatttttttt gtaactttaa
      841 tgcaacttca agattaattg ctgattctaa attacctgta cttattcccg ttgctcctgt
      901 tgttacttta gcttttgttt gactagctcc aggtccaatt ggctctttgt tgctatcgcc
      961 acgtcgttga tgtcctgcgt caatcacaat tacacgacta tcaccaacga ttggtaattg
     1021 aactgttcgc aaagattccg gaacaagtac ttcgccttct ttttcaacaa atgccgtttg
     1081 ttgagcttca actgcttcag ttttgattaa ttcagaatca ttattttgtt tacagccact
     1141 aaaacataac ataattagga aaatactgag caatttcttc attatttcac ccactcttcc
     1201 tcattatatc atagattagc tattaataat tgcctctaat tcaccatcaa tcatctcttt
     1261 taaagtttca cagctacgat caaatttatg ttgatctaca ttatttaaat gcagtgtcat
     1321 gatcttacta atcccttgac gattaatgat tgcaggtacc ccgatatata gcccttcttg
     1381 cttatattca ccttgttgat atgcagaaac tgttaatacg gcattttcat cacttaaaat
     1441 tgctgtaatt aaacgattta aagatagacc tataccataa taagttgcat ttttacgttt
     1501 aattatctca tatgcagctt gttgtacttc tttataaatt ttatgcatct cgttcatatc
     1561 gatattcatt tccacaatat attccatcat actcttgcag ccaatataag tattcatcca
     1621 tggtacaaat gaagaatcac catgttcacc taatatatac gcatgaatat ttgttgagga
     1681 aatgttcaaa tattcactta ataaataacg taatctagct gtatctaaaa tagtcccact
     1741 accaattaca cggcttgttg gtaatcctga tactttttgg actacataac tcatcaaatc
     1801 aacaggatta cttgcaacga taataattcc atcaaatcca ctagccataa tttgctcagt
     1861 aatagatttc ataatttttg cattaacact agctaattcc aatcttgttt gatttggttt
     1921 ttgagcagct ccagcagtaa ttacaacaat atcagcatct ttacattcat cataaccgcc
     1981 agctttaacc tttaaactac ttttactata tggtagaccg tggctaatat ccattgcctc
     2041 tccaattgct ttttcttgat ccacatctat taatacaagt tcatcaatac caccagtatt
     2101 taaaatagaa tatgccattg acattcctac aaaaccagtc cctactaata caaccttttt
     2161 caaatcatca tcttgtctca ttgttagttc ctccttacaa aaccataata gtatttatta
     2221 ctaaaattgt catttgatat gctctattaa tttattgtac tcatttatta taaaattaaa
     2281 ccaggataac aattctttag tcataaaatt tcttacaaat taataagtgg taacgaatct
     2341 ttctaaatcc atgatagtat aataaaaggc tatagtatta tttaataaca tactataacc
     2401 actatctttt aaatagattc tttttcaact ataaataatt tattttgtaa ccaaaaaggg
     2461 ttcttattaa ttaagaacct cttaatctat agcattacta atgaggctat ataaccaacc
     2521 actaaagcaa taacatacga aataaataat attttaaatg tgtctttttt attctcatta
     2581 tattttatta aagctaatgt tcctacaccg gcacttgtcg ttaaacctgt cagtaatgca
     2641 ccaaaactta aaccaccgga cacatataat tgtgtcagta taactgaacc agcacaatta
     2701 gggataaaac caataactcc cgcaatgatt ggctggaaga tccaatttgt ttttaaaagg
     2761 ttggttactg tttcttcacc aattcccgca ataattaatg taaagacgat atttgttaat
     2821 aaaataaaga taaaaatatt gagtgtatgt cgtaaagccg gtaaccaaat accattttga
     2881 caaccacaat cacaaggctc tacttgtaaa tattcataat catcatgact tcgatgattc
     2941 aaacgatcta ctacataacc ggtaactgta cctaaaacaa tttttaaaac aattaaaaca
     3001 actaacattt cccataaatc aggatgtact actaaaatag ccaaagcttc atcacttgtg
     3061 gcaataaaga ttgataataa agttcccatt gtcaagtaac gcatcgaata taatcgggcc
     3121 gcaataactg aaaaaccaca ctgagggata cttcctaaaa tcgctccgat aaaaggaccg
     3181 acttttccaa actccattac cttattgaca acatcacgat tagatatgta ttctactaat
     3241 aagcaggcca aaaataatac tggtactgtc atcagggatt cttgaattgg ttctaataat
     3301 atttccatta aatcactcct atctcaattg ttagactagc ataactttta tatagacttt
     3361 gtttagttta ccatattctt atataatttc aaacttattt ctctattata cgctcaatta
     3421 tacaaaaata aagcgctgtt tttgtcttaa cataatattt ataatcctta tcttgatgat
     3481 ttaagaaata ttagctaaaa tggaatcatt aaataacaaa gggatcgaga ataatgaaaa
     3541 taacaaaaaa gcagttaaat aaaataaaaa tgattgtttg tgatcttgat ggcacactgt
     3601 ttaaccataa aaaagaaata tc
//
LOCUS       DAOMTA010000227         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_904_length_3621_cov_2.426528, whole genome shotgun sequence.
ACCESSION   DAOMTA010000227 DAOMTA010000000
VERSION     DAOMTA010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_3621_cov_2.426528"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLK68_07210"
     CDS             <1..657
                     /locus_tag="PLK68_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90235.1"
                     /translation="NKMLALVGPSGGGKSTIANLLTRFWDVDSGSINIRGTNIKNVKL
                     ADLMNHISMVFQRVYLFQDTIYNNISIGRLEATEEEVIEAAKKARCYDFIMALPNGFQ
                     TIIGEGGTSLSGGEKQRISIARCILKDAPIIILDEATASIDGDNENYIQEAITQLCQG
                     KTLLVIAHRLNTIRYADKILVIADGKIAQSGSHNQLIKQTGIYQDFINVRNNTKGWSQ
                     "
     gene            complement(1762..2118)
                     /locus_tag="PLK68_07215"
     CDS             complement(1762..2118)
                     /locus_tag="PLK68_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRM90236.1"
                     /translation="MGEALEDYLEAILILSNQNKAVRSVDLAVYRGYSRASISYAVKE
                     LRNKNYLEVNKDGHLKLTSSGQVLANGIYERHCFFKKLLIAAGVSSNQAEIEACKMEH
                     GISDDSFEKLKTLLDF"
     gene            complement(2222..>3621)
                     /locus_tag="PLK68_07220"
     CDS             complement(2222..>3621)
                     /locus_tag="PLK68_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM90237.1"
                     /translation="EITDGQALINESSFTGEPLSKAVGKDDTVFAGTIVEEGKIYVKV
                     RNLQKESRINKIVDMIDVNETLKAGVQSRAEHLADSIVPFSFFGFFGLLLWTRSLTRA
                     TSILLVDYSCAIKLTTSISIISAMQEAGKHSIMVKGGKYLEAMAQADTIVFDKTGTLT
                     NATPFVQEVTPLDDYSRDEVLRIAACLEEHFPHSVANAIVKQAQDEELLHQEEHAEVE
                     YIIAHGIATNLHGQRAIIGSEHFVFDDEQIEKPPEIVQLISKLHSKGASSLIYLAIGQ
                     RLAGIISIYDPLKPEAKHVINNLREIGFKKVIMLTGDCENAAGHIANELGIDDYKAGI
                     LPEDKAEYIRLLKAKGKKVVMVGDGVNDTPALSSADVSISMQDSSDIARELADVTLTS
                     SRLDEIVEFKKISILLMQRIKHNYTNIVAFNSLLILSGLIGLLQPNTSAFLHNTSTFL
                     FSAVSTKPLLYKKEN"
BASE COUNT         1199 a          626 c          499 g         1297 t
ORIGIN      
        1 aataagatgc ttgctcttgt gggtccatca ggaggcggta aaagtacgat tgctaatcta
       61 ttaacacgtt tttgggacgt tgatagcggg tcaataaaca tccgtggaac aaatatcaaa
      121 aacgttaaac ttgctgattt aatgaatcat atcagtatgg ttttccaacg tgtttattta
      181 tttcaggata ctatctataa taatattagt atcggacgac ttgaagctac ggaagaagaa
      241 gtcattgaag cagctaaaaa agctcggtgt tatgatttta tcatggcact gccaaatggt
      301 tttcaaacaa ttattgggga aggtggtaca agcctttccg gtggtgaaaa acaacgaatt
      361 tcaattgctc gatgtatttt aaaagatgct cctattatta tccttgatga agcaactgcc
      421 agtattgatg gtgataacga aaattatatc caagaagcaa ttacacagtt atgtcaagga
      481 aaaaccttat tagtaatagc acatcgttta aatactattc gctacgcaga taagattctt
      541 gtcatagctg atggaaaaat tgcacaaagc ggaagccata accagttaat caaacaaact
      601 ggaatatacc aagattttat caatgtccgt aataatacca aaggctggag ccaataaaaa
      661 catgacttat gtcatgtttt tattgtttat taagttaatc aacaaaataa tataattatt
      721 aaataaagtg atgagttatt atgaaaaact acctatacat caatttactt agttgattct
      781 tttaataatg attttggtga atttttatta ccagaaatct taaaatcatt ttattctagc
      841 tgtaaaagta gatttatgat aataaggtat attagaaaat tctaaagacc ctgtaaaaag
      901 aatttataat ttttagctga tttggcttag aatttttctt agttatatta ccattagatt
      961 ttttatcatt ttaaaatctt tctcatttcc tattgtatcg ctaatggtgt tatccatcat
     1021 gatattatcc tttggaggcg agctcctgtc tgaatgcttt agtattaaca aaagacccag
     1081 atacgattga agcaataatt catgactttg cagctgttgt tgattttata gctccattat
     1141 tctttctact attaaacagt attatccaat ttggattcaa ccgcatagct attgaaatac
     1201 ttttccttat taattttata atcaccttta tttttttctc ttttttatta tagataataa
     1261 aggataattt aaaatacatt tgttacgtat gaatgtttct agcaacgtgc aattttaata
     1321 tgcttgtatt tccttttcta ttttatgcaa ttaaatcatt atgtttttga acctgtatat
     1381 ttttatcaat aacggctatg atatgtttag aggtgagtga cattaaatat caaaacttac
     1441 atgaattaat ccagcgcagt tctagttctc gcgaatattt tttatcatta ccagttgata
     1501 cacaactaag attacatgtg cttaatgatt atattcattc tctacatcaa ttacacattt
     1561 atgctgaaat cagcaaataa cgaaattttc ttataaaaat atataccatt aaaatccaga
     1621 taaatgaacg gataaaatca aaaccaaaaa tatttgattt tgtcttttta ttttaaaaca
     1681 tattaacagc atttttttaa taaatttcta ttcaatcaat ttttacaata tctatgttta
     1741 tactcaaaca aaaattccat gctaaaaatc taacaacgtt ttcaactttt caaaactgtc
     1801 atcactaatt ccatgttcca ttttacaagc ttctatttct gcttggtttg aacttacgcc
     1861 agcagcgatt aaaagttttt taaaaaaaca gtgtcgttca taaattccat tagcaagtac
     1921 ttgaccactc gatgttaact tgagatgacc atctttattt acttctaaat aatttttatt
     1981 tctaagctct ttaactgcat aactaatact tgccctagaa tatcctcgat acactgctaa
     2041 atcaacagaa cgaacggctt tattttgatt agataaaatt aaaatagctt ctaaataatc
     2101 ttccagtgct tctcccataa ccacttctcc cttaaattta ttaatttttt atagtataga
     2161 catttattta tcttataatt caattgatta taaaagcaag taaggaaatc tccctacttg
     2221 cttaattttc ttttttataa agaagcggtt ttgttgagac agcagaaaat aggaatgttg
     2281 aagtattgtg taaaaatgca cttgtattag gctgtaataa tccaattagt ccagataaaa
     2341 ttaataaact attaaatgcc acgatattag tataattatg ttttattctt tgcattagta
     2401 atatcgaaat ctttttaaat tcaacaattt catccaaacg acttgaagtc aatgtcacat
     2461 ctgctagttc acgggcaata tcggaactat cttgcattga aatagataca tctgcacttg
     2521 atagtgccgg agtatcattt acgccatcac ccaccatcac gactttttta cctttcgctt
     2581 ttaacagtct tatatactca gctttatctt ctggtaaaat accagcttta taatcatcta
     2641 ttcctagttc atttgcaata tgccctgcag cgttttcaca atctccagtt aacataataa
     2701 cctttttaaa accaatctcc cgtaaattat taattacatg cttggcttcc ggcttgagcg
     2761 gatcataaat actaatgatt cctgctaggc gttgacctat tgctaaataa atcaatgagc
     2821 tagctccttt actatgtaat ttgctgatca actgaactat ttcagggggt ttttcaatct
     2881 gttcatcatc aaaaacaaaa tgctcactac caataattgc tctttgacca tgtaaattag
     2941 tagcaatacc atgagcaata atatattcta cttcagcatg ttcttcttga tgtaacaatt
     3001 cctcatcttg ggcttgctta acaattgcgt tagcaacact gtgagggaaa tgttcttcaa
     3061 gacaagctgc aattctaagt acttcatcac gactgtagtc atctagtggt gttacttctt
     3121 gaacgaatgg agtcgcatta gttaaagttc ctgttttatc aaagacaatt gtatcggctt
     3181 gagccatcgc ttcaagatat ttaccacctt taaccatgat tgaatgtttt ccagcctctt
     3241 gcattgcact gatgatagaa attgatgtag tcaatttaat tgcacacgaa taatcaacta
     3301 ataaaattga agtcgctctt gttagacttc ttgtccataa tagcaaccca aagaagccaa
     3361 aaaagctaaa tggaacaatc gaatcagcaa gatgttcagc tcgcgattga acaccagctt
     3421 ttaatgtttc attaacatcg atcatatcaa caattttatt aatacgactt tctttttgta
     3481 gatttctaac ttttacataa atttttcctt cttctacaat tgttcctgca aaaacagtat
     3541 catccttacc aaccgcttta cttagaggct caccagtaaa acttgattca ttgatcaaag
     3601 cctgaccatc ggttatttct c
//
LOCUS       DAOMTA010000228         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_908_length_3615_cov_2.252809, whole genome shotgun sequence.
ACCESSION   DAOMTA010000228 DAOMTA010000000
VERSION     DAOMTA010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_908_length_3615_cov_2.252809"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLK68_07225"
     CDS             <1..244
                     /locus_tag="PLK68_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90238.1"
                     /translation="YQSLSGGQLQRLSLARAFLHDAPLILLDEPTSNLDSLNEALVLK
                     SLSDNHQGKTIIMVSHRPSSLKICDRIIKIEQGRQS"
     gene            244..537
                     /locus_tag="PLK68_07230"
     CDS             244..537
                     /locus_tag="PLK68_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRM90239.1"
                     /translation="MTIDQEKELISIMIEIYEDGNKVDLSDLKNYAFKRIEFCPRKEE
                     KTFCSSCPIHCYQKTYRQQIREVMKYSGKRIIFKHPVIAFKHVINTLKYKIMS"
     assembly_gap    578..639
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <640..1123
                     /locus_tag="PLK68_07235"
     CDS             <640..1123
                     /locus_tag="PLK68_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90240.1"
                     /translation="ETQRLIIRELEIKDAMRLSEYRDKREVAFYQSWWRYPYQKALKR
                     VEYCVKHPFDGSRGNYQLGVVLKENNILIGDYFLEVLESNSITIGYTFDSDYWQHGYA
                     IESMRALLLELKNRYNFKIVFAHVYDDNIRSIRLLKNLGFVQYETSKIMGDIGFKLRL
                     "
     gene            1252..3444
                     /locus_tag="PLK68_07240"
     CDS             1252..3444
                     /locus_tag="PLK68_07240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HRM90241.1"
                     /translation="MDLTVAKTKTKIKNTGMTYVSIFGLTLLLGLVKIYDTSLCFVLP
                     MLVVSFMCGYRHIIMYMTALLLVSYISYDNYMLLLVSITSVIIMQVMMYLKFVKSKYL
                     ALVVTLVSLLFLYIYQYNYIEVLVILSFTLLHSLLYLEVVPLFIHNTIEVYTNKRMMI
                     LSTMIMLAIISLVELNQVYMMILLRFYLLLSVYYLGINSTMPTILYISIILMFINPLL
                     KEEILSLILPFSIFFMYKPENKLVCSTVYLVSHLVLPFFITYDYYYHSFIIVVSATLF
                     LFVPTFKHKPKLLSDDFKNITSRNKLIQRATTFASLFKQLTDIFQEANRNVNVGEFVG
                     YVYEDVCSKCPSRDLCFYRDGNVSRLGKLINKGFKTNYTKEDINYINSNCISPNRFLK
                     SISEYKDSYEKIKRVNQENSHLKKDLFYEFSLLSEVFDNFSNSLEQTPLNDDSLKEHL
                     LGYQFNITYLYRHQIGNNVYTLELGLMDISEEEVVNELVPIVESYLNETLEIVSLKDS
                     MHHLGYTSLVLKHQQNYSLQYGFQQYALEPLNCGDSFSAFHQDNLHYLALSDGMGQGK
                     MAANESKLTLEVLSKLILNGIGLKDTIDSINALLKIKNRNDMFTTLDLCNINLANARM
                     KLIKYGANPSYHIRSGMVEKIATNSLPVGVVSKLKMVSYEMPLKNNDLIIMSSDGTGN
                     DFEQIVNDNVHLYCDQHPQEIATFLMDQVLEKNNLDDISIIVIKVVNN"
BASE COUNT         1245 a          444 c          587 g         1277 t
ORIGIN      
        1 ttatcagtct ttatcagggg gacagctgca aagattatct ctagctagag cttttttaca
       61 tgatgcacca ttgattctat tggatgaacc aactagtaat cttgatagct taaatgaagc
      121 tttagtatta aaatcactaa gtgataatca tcaagggaaa accattatta tggttagtca
      181 ccgaccttca tcattaaaaa tctgtgatcg aattattaaa atagaacagg ggcgacagag
      241 ttaatgacga ttgatcaaga aaaagaatta attagtataa tgattgaaat ctatgaagat
      301 ggtaataaag tcgatttaag tgatttaaaa aattatgctt ttaagcgaat tgaattttgc
      361 cctcgtaaag aggaaaagac attttgttca tcatgcccga ttcattgcta tcaaaaaaca
      421 tatcggcagc aaattcgaga agttatgaaa tattcaggaa aaagaattat cttcaaacat
      481 ccagtcattg ccttcaaaca tgtaataaat acgctaaaat ataaaataat gagctaaaat
      541 aaaccgattg tactaggtaa tgatattatg atatgatnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gaaacacaac gcttgataat
      661 tagagagttg gaaataaaag atgctatgcg tttgagtgaa tatcgtgata aaagggaagt
      721 tgctttctat caatcatggt ggcggtaccc ttaccagaaa gccttaaaaa gagtagagta
      781 ttgtgttaaa catccttttg atggaagtcg tggtaattat caattaggtg tagtattaaa
      841 agaaaataat attttgatag gtgattattt tttagaggtc ttagaatcta attctattac
      901 gatcggttat acttttgata gtgattattg gcaacatggc tatgcaattg aatcgatgcg
      961 agcactttta ttggagttaa aaaatagata taattttaaa attgtttttg ctcatgttta
     1021 tgatgacaac ataagatcta ttcgtttgct taaaaatctt ggatttgttc aatatgaaac
     1081 atctaagatc atgggggata ttggatttaa attaagatta taacaactgg tgtaaaacta
     1141 gttgtttttt tgtttgtaaa tctataagtt tttattttat tttaaaataa ataaaaagtg
     1201 acaaattatt ctactgtaag tatctataat cgatattacg aggtgaatga aatggatttg
     1261 acagtagcaa aaacaaaaac aaaaataaag aatactggaa tgacttatgt ttcaatcttt
     1321 ggattaaccc tccttctagg tttagtaaaa atatatgaca catcactgtg ttttgttctc
     1381 cccatgctag tagtatcatt tatgtgcggc tatcgacata tcattatgta catgaccgct
     1441 ttattattgg tatcatacat atcttatgat aactatatgc tattattagt ttcgattact
     1501 agcgtgataa ttatgcaagt gatgatgtac ttaaaatttg tcaaaagtaa gtatttagcc
     1561 ctagtagtta ctttggtaag tttactcttt ctttacattt atcaatataa ttatattgaa
     1621 gtattagtta tattatcctt tacactatta catagtctac tgtacctaga agtagtgcct
     1681 ctctttatcc ataacacgat tgaagtatat accaataaac ggatgatgat tctttcaaca
     1741 atgataatgt tagccatcat ttcattagtt gagctcaatc aagtctatat gatgatatta
     1801 ctaaggttct atttattact aagtgtctat tatttaggaa ttaatagtac catgcctacg
     1861 attctatata tatcaatcat attaatgttt attaatccct tattaaaaga ggaaatacta
     1921 tcattaatcc ttccttttag tattttcttt atgtataagc cggaaaataa attagtctgt
     1981 tcaaccgttt atcttgtaag tcatctagtt ctccctttct ttattacata tgattattac
     2041 tatcatagtt ttattattgt agtaagcgca acgttgtttt tatttgtacc tacctttaaa
     2101 cataagccta aattacttag tgatgatttt aaaaatatca caagtcgaaa caaattaatt
     2161 caacgtgcta ccacattcgc ctctttattt aaacaattaa cagatatctt tcaagaagca
     2221 aatcgtaatg taaatgtcgg tgagtttgtt ggctatgttt atgaggatgt atgttccaaa
     2281 tgtcctagtc gggatttatg tttttatcgt gatgggaatg taagtcgatt aggaaaattg
     2341 attaataaag gatttaagac taactacaca aaagaagata ttaactatat taatagtaat
     2401 tgcattagtc caaatcgctt cttaaagagt atcagtgaat ataaggatag ttatgaaaag
     2461 ataaaaagag ttaatcagga aaattcgcat ttaaagaaag atttgtttta tgaattttcg
     2521 ttattgagtg aggtgtttga taattttagc aattctttag aacaaacgcc attaaatgat
     2581 gatagtttaa aagagcattt attaggctat caatttaata ttacatattt ataccgtcat
     2641 caaatcggta ataatgttta tactttagaa ttaggattaa tggatattag tgaggaggag
     2701 gttgttaatg aactagttcc aatagtagag tcatatttaa atgaaacatt agaaatagtt
     2761 tctctaaagg attcaatgca tcatcttggt tacacgtcat tagttttaaa acatcaacaa
     2821 aattattcat tgcagtatgg ttttcagcaa tatgctttag agcctcttaa ttgtggtgat
     2881 tcgtttagcg cgttccatca agataatctt cattatttag cattgagtga tggaatgggt
     2941 caaggcaaga tggctgctaa tgaaagtaag ttgacattag aagtactatc aaaattaatt
     3001 ttaaatggaa ttggattaaa agatacaatt gattcaatta atgctttatt aaaaataaaa
     3061 aatcgtaacg acatgtttac gactttagat ctatgtaata ttaatcttgc taatgcccga
     3121 atgaagttaa tcaaatatgg tgctaatcca agttatcata ttcgtagtgg aatggttgaa
     3181 aaaattgcaa ctaattcatt accagttgga gttgtttcta aattaaaaat ggttagttat
     3241 gagatgccgc taaaaaataa tgatttaatt attatgtcaa gtgatggcac aggaaatgat
     3301 tttgaacaga tcgttaatga taatgttcac ttatattgtg atcagcaccc acaagaaata
     3361 gctacttttt taatggatca agtattagag aaaaataatt tagatgatat aagtattata
     3421 gttattaaag tggtaaataa ttaatttagt ggacttcatt agtttgaatg aatcagcgat
     3481 atgtacccat aattttggac atgtttattt atgaactagg caatacatat ggatgtttcc
     3541 ttgtatctta caggagatat ccgttttgtt tttcttgaat tctttggttg ttgtaatgat
     3601 ctatatattc atcta
//
LOCUS       DAOMTA010000229         3607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_918_length_3607_cov_1.918637, whole genome shotgun sequence.
ACCESSION   DAOMTA010000229 DAOMTA010000000
VERSION     DAOMTA010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_918_length_3607_cov_1.918637"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLK68_07245"
     CDS             <1..681
                     /locus_tag="PLK68_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRM90242.1"
                     /translation="KDLLSYVHKECDNMNKIPYRYDIVGSFLRSERLKHARENFNNNL
                     ISKNELKKIENEEILKLVKKEEAAGLKAVSDGEFRRSYWHLDFLAGLTGVKKIEAEKW
                     SVDFKGVQPKAATLKIIDKIDFEDHEFLEHFEYLNSIIENGVTAKMTIPAPTMLHLIA
                     CVRTKEYQPIARYQDDEQLIVDLAMAYQKIIQAFYDRGCRYLQLDDTSWGEFCSKEKR
                     DMLIVGLM"
     gene            636..1148
                     /locus_tag="PLK68_07250"
     CDS             636..1148
                     /locus_tag="PLK68_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90243.1"
                     /translation="MFKRKTGYANCGIDVGALVKKYVYLINLSIVNKPDDMNITIHIC
                     RGNFRSTWFSSGGYEPVAKELFGNCKVDEFFLEYDSDRSGDFTPLRFIKDQVVVLGLI
                     TSKFSELEDKEMIKARINEASQFVALDQLCLSTQCGFASTEEGNNLTEDQQWAKIALV
                     KEISEEVWGK"
     gene            1148..3415
                     /gene="metE"
                     /locus_tag="PLK68_07255"
     CDS             1148..3415
                     /gene="metE"
                     /locus_tag="PLK68_07255"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM90244.1"
                     /translation="MKTAVIGYPRIGSNRELKFATERYLKGQIEQSELLQIAQNLRKE
                     HWVEQDNQKIDYISSNDFSFYDNVLDTAYLLNIIPKRYQELQLNSLDQYFAMARGYQG
                     ENGDVKALAMKKWFNTNYHYLVPEVDDQVEISLNGTKIFDEFQEALDLGIKTKPVIVG
                     PYTLMKLLRFTGTLTYQDILDDLINGYLEVIKKLRDLGASWIEVDEPALVFDLSDKDI
                     ILFKEIYTKILDNKESVKILLQTYFGDIRGCYQTVLNLDFDGIGLDFIEGKENLTLLD
                     RYGFDQNKILFAGVVNGKNIWKNEYEKTVSLIEKIKNNVNKIVLNTSCSLIHVPYTIE
                     NERNLEQIYKNNFSFAQEKLKELFQLKVIMRIGKGHSYYVDNLAFFKEPNNRTNEAVQ
                     ARIAALKKEDFIRLPEFEVRRQIQKEKFKLPILPTTTIGSFPQTKEVRANRRAYRNGD
                     IDEQTYAEFNHQMIKEWIKYQEEIDLDVLVHGEFERNDMVEYFGENLDGYLFTENGWV
                     QSYGTRCVKPPVIWGDISRVRPLTVDYAVYTQALTKKPVKGMLTGPVTMLNWSFPRID
                     ISLAETTLQLALAIKDEVLDLENHGIKIIQIDEAALREKLPLRKRDWQSEYLDWAIPA
                     YRLVHSSVQADTQIHSHMCYSEFDDIIQAIEDMDSDVISFEASRSDLTLIDTLNRVNF
                     KTEIGPGVYDIHSPRIPSVEELERVLTSMLEKLSASKLWINPDCGLKTRAYPETLASL
                     KNMVEATKNIRAKLQ"
     gene            3459..>3607
                     /locus_tag="PLK68_07260"
     CDS             3459..>3607
                     /locus_tag="PLK68_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM90245.1"
                     /translation="MTLQQLRYVITVAQKGSISEAAKELFISQPSLSNAIKELEKEMK
                     IIIFSR"
BASE COUNT         1340 a          435 c          690 g         1142 t
ORIGIN      
        1 aaagatttgt tatcctatgt acacaaggag tgtgataaca tgaataaaat accgtatcga
       61 tatgatatag ttggaagttt tttaagaagc gagcgtttaa aacatgctcg tgagaatttc
      121 aataataatt taattagtaa aaatgaatta aaaaaaattg aaaatgaaga gattttaaaa
      181 ttagtaaaaa aagaagaggc agctggttta aaagctgtaa gtgatggtga atttcgtcgg
      241 agttattggc atcttgattt tttggcaggt ttaacaggtg ttaaaaaaat tgaagctgaa
      301 aaatggtcag ttgattttaa aggggttcag cccaaggcgg cgactttaaa aattattgat
      361 aaaattgatt ttgaagatca tgaattttta gaacatttcg aatatttaaa ttcaatcatt
      421 gaaaatggtg taacagcaaa aatgacgatt ccagcaccaa cgatgctaca tttaattgcc
      481 tgcgttagaa caaaagaata tcagccaatt gcaagatatc aggatgatga acaattgatt
      541 gtagatttag ctatggctta tcaaaagatt attcaggctt tttatgatcg aggatgtcgg
      601 tatttacagc ttgatgatac ttcatgggga gaattttgtt caaaagaaaa acgggatatg
      661 ctaattgtgg gattgatgta ggagcactgg tcaaaaaata tgtatattta attaacttat
      721 cgattgttaa taagccagat gatatgaata taacaattca tatttgtcgt ggtaacttca
      781 gatcaacttg gttttcatca ggggggtatg aaccagttgc taaggaatta tttggtaact
      841 gtaaggtaga tgagtttttc ttagagtatg atagtgatcg ttcaggggat tttacaccac
      901 ttagatttat taaagatcaa gtagttgttt taggattaat tacatcaaaa ttttcagaat
      961 tggaagataa ggaaatgata aaagcaagaa ttaacgaagc tagtcagttt gtagctttag
     1021 atcaattatg cttgagtacc caatgtggct ttgcatcaac tgaagaagga aataatttaa
     1081 ctgaagatca gcagtgggct aaaattgcat tagtcaaaga aatttctgaa gaagtgtggg
     1141 gaaaataatg aaaacagcag ttattggata tccaagaatt ggatcaaatc gagaattaaa
     1201 atttgctaca gagcggtatt taaagggaca gattgaacaa agtgagttat tacaaattgc
     1261 ccaaaattta agaaaagaac attgggttga acaagataac caaaaaatag attatatttc
     1321 ttcaaacgat ttttcatttt atgataatgt tttagataca gcctatttac taaatatcat
     1381 tcctaaacga tatcaagaat tacaattgaa ttcattagat caatattttg caatggcacg
     1441 aggctatcaa ggggaaaatg gagatgttaa agctttagca atgaaaaaat ggtttaatac
     1501 taattatcat tatttagtac cggaagttga tgatcaagtt gaaattagct taaatggaac
     1561 taagattttt gatgagtttc aagaagcact tgatttaggg attaagacca aaccagtcat
     1621 tgttggacca tatactttaa tgaaattatt acgttttaca gggacactaa cttatcaaga
     1681 tattcttgat gatcttataa atgggtattt agaggttatt aagaaattga gggatttagg
     1741 tgcttcatgg attgaggttg atgaaccagc actagttttt gatttaagtg ataaagatat
     1801 tatcttgttt aaagaaatat atactaaaat tttagataat aaagagtcag taaaaatttt
     1861 attacagaca tattttggag atattcgtgg ctgttatcaa acagttttaa atttagattt
     1921 tgatggaatt ggattagatt ttattgaagg taaggaaaat ttaactttat tagatcgtta
     1981 cggttttgat caaaataaaa tcttatttgc aggagtagtt aatggtaaaa atatttggaa
     2041 gaatgaatat gaaaaaacag tttctttaat tgaaaaaata aaaaataatg tcaataaaat
     2101 agttttaaat acatcatgtt ccttgattca tgtcccttat acgattgaaa atgaaaggaa
     2161 tttagagcaa atatataaaa ataatttttc ttttgcacaa gaaaaattaa aagaattatt
     2221 tcaattaaag gtaattatgc gaattggaaa aggacatagt tattatgttg ataatttagc
     2281 attctttaaa gagccaaata atcgtactaa tgaagcagtc caagcacgaa ttgcagcact
     2341 gaaaaaagaa gactttattc gcctaccgga atttgaagta cgtcgccaaa ttcaaaaaga
     2401 aaaatttaaa ttaccaattt taccaactac aacaattggt tcatttcctc aaaccaaaga
     2461 agtaagagct aatcgccgag cttatcgtaa tggtgacatt gatgaacaga catatgcgga
     2521 atttaatcat cagatgatta aagaatggat caagtatcaa gaagaaatag acttagatgt
     2581 tttagtacat ggagaatttg aaagaaatga tatggttgaa tattttggtg agaatctaga
     2641 tggatattta tttactgaga atggctgggt acaatcatat ggtactcgtt gtgttaaacc
     2701 gccagttatt tggggtgata tttctcgagt gcgcccgcta acagtagatt atgcagtata
     2761 tacacaagct ctaactaaaa aacctgttaa aggaatgttg accggtccag taacaatgtt
     2821 aaactggtca tttccgcgga ttgatatttc tttggcagaa acaactttac aactggcact
     2881 tgcaatcaaa gacgaggttt tagatcttga aaatcatggc attaaaatta ttcaaattga
     2941 tgaagcagca ctgagagaaa aattgccatt acgaaaaagg gattggcaaa gtgaatatct
     3001 tgattgggca attccagcat atcgcttagt tcatagtagt gttcaagcag atacacaaat
     3061 tcatagccat atgtgttaca gtgaatttga tgatattatt caagcaattg aagatatgga
     3121 tagtgatgtt atttcttttg aagcttcacg ttctgatcta acattgatcg atactttaaa
     3181 tagagttaat tttaaaactg agatcgggcc aggagtctat gatattcatt ctccaagaat
     3241 tcctagtgtt gaagaattag aaagagtttt aacttcgatg cttgaaaaat tgtctgccag
     3301 caaattatgg atcaatccag attgtggctt aaagacccga gcttatccag aaactttagc
     3361 tagtttaaaa aatatggtcg aagcaactaa aaatattcgt gccaaacttc aataactgag
     3421 taaatagtgt tagaataagg ccaaaggaag tgaagaaaat gacattacaa caattacgct
     3481 atgtgattac ggtagctcaa aaaggctcaa ttagtgaagc agctaaagaa ttatttattt
     3541 cacagcctag tttatctaat gcaattaaag aattagagaa agagatgaaa attatcattt
     3601 tctcacg
//
LOCUS       DAOMTA010000230         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_927_length_3594_cov_2.169539, whole genome shotgun sequence.
ACCESSION   DAOMTA010000230 DAOMTA010000000
VERSION     DAOMTA010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_3594_cov_2.169539"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLK68_07265"
     CDS             <1..399
                     /locus_tag="PLK68_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90246.1"
                     /translation="KEECRPIDIISGFTEESWQIKSCQVGGYLIGVLKGIQYAIDNNY
                     DEVTIFYNFEGADKYTKIRNSEGLSDVVKTYLSGFKNLEQKIDIKFEKANMNLFIPRT
                     NLQRAYKISRKATQEEFDEFHILNKKNFKP"
     gene            581..1276
                     /locus_tag="PLK68_07270"
     CDS             581..1276
                     /locus_tag="PLK68_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90247.1"
                     /translation="MLNDNDKSNNCKRKVNIKLSIENVKAVLNRVSELMLDHAYSVGA
                     NISSFSASVIRMSDGSLESGDYKFIKSVCKSNSNEDYICRILEAVNMLKNEKQKNVII
                     LRYFYNATFNEIRYGYYDPVDDVEFEPIWQVYKLRDNALAELIYLIDDVLVYEDENIR
                     SKNTKTVKESKSKERIPVNYVAKNGMHGTIRLKDYEDFLNWSKRMKIFKEVELTDETI
                     KNVKAVFGVKGVA"
     gene            1406..2293
                     /locus_tag="PLK68_07275"
     CDS             1406..2293
                     /locus_tag="PLK68_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM90248.1"
                     /translation="MKVISIINEKGGVGKTTASVNISYGLVERLMNDGKRILLIDADA
                     QGNATKFFLPEFKSITLEEFNMLEVPENCDIRSSTKFIKNSLISMLGERNDLNKLLLE
                     GKGVIRECIHQTQYQQLDIIPSIGTELIATDKLLGASTGQRHVILKRALREIRNDYDI
                     VIIDHAPTFNNITVNGLFCSNEIIIPLKPGGFELKGLIDTLEELFDIEDDYECEYKIR
                     ILMNMIPRGVRPAYISFINKIREFYGDTILQTTVGYQDAVASRSTMSGKLLYNSKTGV
                     GEDYRNLVDELIKEFDNEI"
     gene            2335..3180
                     /locus_tag="PLK68_07280"
     CDS             2335..3180
                     /locus_tag="PLK68_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRM90249.1"
                     /translation="MALTNLINADKVSKMNKKPVGLKIIDIDDIEVNQHNQFDINGID
                     ELKSSILEYGLRKPLDVYKIGDRYRISDGERRYTALKELVDEGKIEPEVYCIIYEAPE
                     SELNDRLRLILGNGTRIMSELDKIKIVAELLDIYKQLDPKPKGNKRDWIAPFIGAKSG
                     KTVQKYINAIEKKEKCIDIGMYDEDKKDSCKNKEYGLSELFSDYNKLLKQIDKINKKV
                     ENTSIYHKKIAESDDRDITVEDSLINIHNVIGKFSRCIQISIEIDEQNNDENVLDGQM
                     SIDEI"
     gene            3237..3473
                     /locus_tag="PLK68_07285"
     CDS             3237..3473
                     /locus_tag="PLK68_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90250.1"
                     /translation="MGDLVLRDGDEFFKLSFLKRFLLGTNGIIAGGCFKNIFNGERVK
                     DIDIFFRDKRDLSDVNKKYQDNSDFTLLKNFMNV"
BASE COUNT         1392 a          381 c          744 g         1077 t
ORIGIN      
        1 aaagaagaat gcagaccaat tgatattata agcggtttta cagaagaatc atggcagata
       61 aagagctgtc aggttggagg atatctgata ggggttttga aaggaattca atatgcgata
      121 gataataatt atgatgaagt cacgattttt tacaattttg agggagcaga caagtataca
      181 aaaataagaa acagtgaagg attatcggat gtggtaaaaa catatctttc aggttttaaa
      241 aatctggaac agaagattga tataaagttt gaaaaggcta atatgaacct gtttattcca
      301 agaacaaatt tacagcgagc atataagata tccagaaaag caacgcagga agaatttgat
      361 gaattccata ttctaaacaa gaaaaatttc aagccttaaa ataggatgtt tctataacgg
      421 aatagataaa tatactgtac agaatattaa aaaacagaaa aggtgaaatg tgtcaattgg
      481 tacaaattag aatataagag cattaacata caaaaatcat attatattgt tagaataact
      541 gacacaatgc ttagagttta ctggagaggg gtaatgatcg atgctaaatg ataatgacaa
      601 atctaataac tgcaaaagaa aagtaaatat aaaattatct attgaaaatg ttaaagctgt
      661 tttaaaccga gtaagtgagc tgatgctcga tcatgcctat tcagtaggtg caaatatttc
      721 atcattttct gcctctgtaa taaggatgtc tgacggcagt ttagaaagcg gtgattataa
      781 gtttattaaa agtgtatgca agtcaaatag taatgaagat tatatatgca gaattcttga
      841 agctgtaaat atgttgaaaa atgaaaaaca gaaaaatgta attattcttc gctatttcta
      901 taacgctaca tttaatgaga tacgttatgg ttattatgat ccagtagatg atgttgagtt
      961 tgaacctatc tggcaggtat ataagcttcg tgataatgcg ttggcagagc ttatttatct
     1021 gattgatgat gtactggtct atgaagatga aaatataagg agcaagaaca caaaaactgt
     1081 aaaagaaagt aaaagtaaag agcgaatccc agttaactat gtagctaaga atggaatgca
     1141 cgggactata cgtttaaaag attatgaaga ttttttaaac tggtcaaagc gtatgaagat
     1201 tttcaaagaa gtagaattga ctgatgaaac tattaagaat gtcaaagcag tgttcggggt
     1261 aaagggtgtc gcgtagatgt ttaacatcag atattcgaat ttcattttgt aaaatgagtt
     1321 gatcttatta atttatgaac atttttgcta ttgagagtaa tatgaaaagt cgaaatttaa
     1381 taaaagaaaa aaggagaata gagagatgaa agttataagt attatcaatg aaaagggcgg
     1441 ggttggaaaa accaccgcat cagtaaatat aagttacggg ttagtggaaa gattaatgaa
     1501 cgacggaaaa agaattttgt taatcgatgc agatgcgcag ggcaatgcaa ctaaattttt
     1561 tctgccggag tttaaatcaa ttacactgga agagtttaat atgctggagg taccggaaaa
     1621 ctgcgatatt agaagttcaa ctaagtttat aaaaaattca ctaattagta tgcttggcga
     1681 aagaaatgat ttaaataaac ttctgcttga gggcaaaggg gttattaggg agtgtattca
     1741 tcaaacacag tatcagcagt tggatattat tccttcaatc ggtactgaac ttattgctac
     1801 agacaagttg cttggcgcat cgacaggtca acgacatgtg attttaaaaa gggctttgag
     1861 agaaattaga aatgattatg atatcgtgat tatagatcat gcaccaacat ttaataatat
     1921 tactgtaaat ggtctattct gttcgaatga aattataatt cctcttaaac caggtggatt
     1981 tgaattaaag gggctgatcg atacacttga ggaactgttt gatattgaag acgattatga
     2041 atgtgaatat aaaataagaa tattgatgaa tatgattcct cgtggtgtta gacccgctta
     2101 catcagtttt atcaataaaa tcagagaatt ctatggcgat actattttac agacaacggt
     2161 aggctatcag gatgcagttg cgtcaagatc gactatgagc ggtaaattac tgtataacag
     2221 taaaactggt gttggtgagg attatcgtaa tcttgtagat gaattaatta aagagtttga
     2281 taatgaaatt taaatcctgg taaatttacc aggattatga ggaggagtaa aggtatggca
     2341 cttacaaatt taatcaatgc cgataaagtt agcaagatga ataagaaacc tgtaggacta
     2401 aaaattattg atattgatga tatagaagtg aatcagcata atcagtttga tattaatgga
     2461 attgatgagc ttaaatcatc aattctggaa tatggattaa ggaaaccgtt ggatgtttat
     2521 aaaataggtg acagatacag aataagtgat ggtgaacgta ggtatacagc acttaaggaa
     2581 ttagttgatg agggaaagat agaaccggaa gtatactgta tcatttatga agcaccggaa
     2641 agcgaattaa atgatcgttt gcgcttgatt ttagggaatg gaacaagaat aatgtcggaa
     2701 ctagataaaa taaaaattgt tgctgaactg ttagatatat ataaacaatt ggatcctaaa
     2761 cctaaaggta ataaaagaga ctggattgca ccttttattg gtgcaaaatc tggtaaaacg
     2821 gtacaaaaat atataaatgc aattgaaaag aaagaaaaat gtatcgatat aggaatgtat
     2881 gatgaagata aaaaagatag ctgtaaaaat aaagagtatg gattaagtga actcttttca
     2941 gattacaata agctgttaaa gcagatcgat aagattaata aaaaagttga gaatacttct
     3001 atatatcata aaaagattgc agaatctgat gatagggata ttactgtcga agattctcta
     3061 ataaatattc ataatgtgat tggtaaattt agtagatgta ttcaaatatc aattgagata
     3121 gatgaacaaa ataatgatga aaatgtttta gatggtcaga tgagcataga tgaaatatga
     3181 gtataaagca attacaacgc tggtaaattt accagcgttg gcaaaggagg ggtaatatgg
     3241 gggatttggt gcttagagat ggggatgaat tttttaaatt atcattttta aaaagatttt
     3301 tacttggaac aaatggaatt attgcaggcg gttgtttcaa aaatattttt aatggagagc
     3361 gtgtcaagga tatagatata ttctttagag ataaaaggga ccttagtgat gtcaacaaaa
     3421 agtatcaaga taattctgat tttaccttat taaagaattt tatgaacgta tgaaacacga
     3481 ttataaaaat gttgaatatt ttgaaattat ttttgattaa tttaattgag atgaaatggt
     3541 taattgaaaa tatgaaaaaa gttaggaggt catgaagtat gccaaaaatt acag
//
LOCUS       DAOMTA010000231         3589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_932_length_3589_cov_2.238257, whole genome shotgun sequence.
ACCESSION   DAOMTA010000231 DAOMTA010000000
VERSION     DAOMTA010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_932_length_3589_cov_2.238257"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(545..1501)
                     /locus_tag="PLK68_07290"
     CDS             complement(545..1501)
                     /locus_tag="PLK68_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM90251.1"
                     /translation="MYYLGVDLGGTTIKAGLFDEQLQLLKMVQKNTGASLGADIVLKR
                     IEKCLEILVLKNNLLFEQIDALGIGVPALLNRNDGISLFSPNFNNWHNVKIKEWFEHK
                     WLIPTVIDNDVRMHLYGELYFGAGKGFKNIILIAIGTDLGSGIVVDGHVLYGANDSVG
                     EIGHMNMYRHGRACRCGSSGCLGRYVSAVGMINTFKEKDIDHMSIVNRWVNNCDEITA
                     KMICQAYDLNDSIAVATLKETGEILGYGITNLINLFNPERIIIGGGVSNAGERLLAST
                     REVAAIHALEIASNNCDLVVAELGEQAGMYGAAKYAKRKLIL"
     gene            complement(1594..2871)
                     /locus_tag="PLK68_07295"
     CDS             complement(1594..2871)
                     /locus_tag="PLK68_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRM90252.1"
                     /translation="MIKATILVEELNNKKYDELLNDIYVDTNLLDYQRERYVKAINKY
                     VSLYGDTDVEIYSAPGRSEVGGNHTDHQHGCVLAAAVNLDAIAVVGRVDNKIKVLSDD
                     FDIAPINLEDLEIKKAEEGTSEALIRGVCARLKELGYNVGGFNAFITSDVLMGAGLSS
                     SAAFETIIGTIISGLYNDMTIDPVVIAQVGQYAENVYFGKPCGLMDQCASSVGSLINI
                     DFNDVAKPIVNKVDVDFSKFGHSLCIVDTKGSHADLTDEYAAIPMEMKKVANYFGKEF
                     LREVDEEDFFNDIAGARKACQDRAVLRAIHLFEENKRVDQEVKALNNSDFETFKKVVK
                     ESGDSSYKFLQNVYANCDVQNQSVSIGLAMSEKIIGRNGVCRVHGGGFAGTIQAFVKD
                     EFVTAYKTEIERVFGKGSCHVLKVRKYGGKKVI"
     gene            complement(2871..>3589)
                     /locus_tag="PLK68_07300"
     CDS             complement(2871..>3589)
                     /locus_tag="PLK68_07300"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRM90253.1"
                     /translation="LHGGVKGFSYQIFDYQILEDSIEFTYLSKDGEEGYPGNLAFKAI
                     YTLNKDTLIVRYQATSDKDTIINITNHSYFNLSGAKEDIYNHQLLVHSDKYACVDSDG
                     LPTGKFNLVKDTPFDFNSMTRIGDVIDSDDEQLKLGAGYDHPFIFNQDKDQAILYHEA
                     TGRKLTVSTSLPGAQIYSANYLDGRIGKYGIVYPRRFALCIETQNLPDAINIEEDPTT
                     ILKKGEVYDEITSYKFEVIK"
BASE COUNT         1224 a          749 c          370 g         1246 t
ORIGIN      
        1 catcgtcata caataactta attctcttat tgacatttcc aaataataat ccaccatttt
       61 ccccttgaac catttctaca ataaatttaa ttgcgagcac cttttatatt attcgtgcaa
      121 aaaatttaat tatctccatt tctttattaa agatataaaa aacaatctgc ttatcaaaaa
      181 tatttttaag gaaaaataac ttatttccca gatcaattat ctaattaaat cctagttatt
      241 tcaaatgatt aaaaatagct atatttcaaa aatttatcat tggccattta aagcttatac
      301 agatcacaat cctcaatttt tcctcattta taacatcaag cttgataact tctctttcct
      361 aattttacta taaacaaaga atatttgtaa taattaccaa atttaaaaag tacaaactgt
      421 tttcacctaa tttgtacttt ttaaacttta cttcttttct cattaaacac agagataaaa
      481 aatactaatc taatattaat aaaaaagatt actattgtat gatttatcta gtttaacgac
      541 ggcatcataa tattagtttt cttttagcgt atttagctgc accatacatt cccgcttgtt
      601 ccccaagttc tgcaaccact agatcacagt tattactagc aatttccaaa gcatgaatag
      661 cagcaacctc tcgagttgaa gctaataacc gctctcctgc atttgatact ccaccaccaa
      721 taataatacg ttcaggatta aataagttaa taaggttagt tatcccataa ccaagtattt
      781 ctcctgtctc tttaagagtc gcaacggcaa tcgaatcatt taaatcataa gcttggcaga
      841 tcatctttgc agtgatctca tcacaattat ttacccatct attaacaata ctcatatgat
      901 caatatcctt ttctttaaac gtattgatca ttccaactgc agaaacgtac ctacccaagc
      961 acccactact accacaacga caagctctcc catgacgata catattcata tgcccaattt
     1021 ctccgacact gtcatttgca ccgtataaaa catgtccatc tactacgatt ccagaaccta
     1081 aatcggtccc aattgcaatt aaaataatat ttttaaaacc tttcccagct ccaaaataca
     1141 attctccata aagatgcatt ctaacatcat tatcaattac agttggaatc agccacttat
     1201 gttcaaacca ttctttgatc ttgacattgt gccaattatt aaaattagga gaaaataaag
     1261 aaataccgtc atttcgattt aacaaagccg gtactcctat acctagagca tctatctgtt
     1321 caaataaaag attattcttt aaaactaata tttctaaaca tttttcaatt ctttttaaaa
     1381 ctatatctgc acccaaactt gctcctgtat ttttttgaac catttttaat aattgtaact
     1441 gttcatcaaa gagtcctgct ttaatagttg ttccgcccaa gtctactccc aaataataca
     1501 tttttcctcc tattaaaaaa gggcatttct atagtgaagc tccattattt taatatgctc
     1561 aaataacaga gttcactttc ttctgcccaa cttttaaatt acttttttac cgccatactt
     1621 gcgaactttt aatacatgac agcttccttt accaaaaact ctttcaattt cagtcttata
     1681 agcagttaca aattcatctt taacaaaagc ttgaatcgtt cccgcaaaac caccaccatg
     1741 aacccgacaa acgccatttc ggccaataat tttttctgac atggctaatc caattgaaac
     1801 actttgattc tgaacatcgc agtttgcata cacattttgc aagaacttat aagaactatc
     1861 tccagattcc ttaacaactt ttttaaatgt ttcaaaatca ctattgttta aagccttaac
     1921 ttcttgatca acccgtttat tttcttcaaa taaatgaatt gctcttaaaa ctgcccggtc
     1981 ttgacaagct tttctagcac ctgctatatc attaaagaaa tcttcctcat ctacttcacg
     2041 taaaaattct ttaccaaaat agttagctac ttttttcatt tccataggaa ttgctgcata
     2101 ttcatcagtt aaatcagcat gtgatccttt tgtatcaaca atacataaac tgtgtccaaa
     2161 tttagaaaaa tcaacatcta ctttattaac aattggctta gccacatcat taaaatcaat
     2221 attaatcaag ctacctactg aactagcaca ttgatccatt aaaccacaag gtttaccaaa
     2281 gtatacattt tcagcatatt gaccaacttg agcaattaca acagggtcaa tagtcatatc
     2341 attatataaa ccagaaataa tagtaccaat aatcgtttca aaagctgctg aagatgataa
     2401 tcctgctccc attaatacat cactagtaat aaaagcatta aaaccaccaa cattatatcc
     2461 taattctttt aaccgagcac ataccccacg aattaaagcc tcagatgttc cttcttcagc
     2521 cttttttatt tctaagtctt ctaaattaat tggcgcaata tcaaaatcat cagataatac
     2581 tttaatttta ttgtcaactc ttccaacaac agcgattgca tcaagattaa ctgctgctgc
     2641 taaaacacaa ccatgctgat gatctgtatg atttcctcct acttcgctac gtcctggagc
     2701 actgtaaatt tctacatcgg tatcaccata caatgataca tatttattga ttgctttaac
     2761 atatcgctca cgttgataat ctaaaagatt tgtatctaca taaatatcat ttaataactc
     2821 atcatacttt ttattattta attcttctac taaaattgtt gctttaatca ttattttatt
     2881 acctcaaatt tatatgatgt tatttcatca tatacttcac ctttctttaa aattgttgtt
     2941 gggtcctctt caatattaat tgcatcggga agattttgag tttcaataca taatgcgaat
     3001 cttcttggat atacaattcc atatttaccg atacgtccat ctaaataatt agcactataa
     3061 atttgagctc caggcaatga tgtacttact gtcaatttac gtccagtagc ttcatgatat
     3121 aatatagctt ggtctttatc ttggttaaaa atgaacggat ggtcataacc ggcacctaat
     3181 ttaagctgct catcatcact atcaattaca tcaccaattc ttgtcattga attaaaatca
     3241 aatggagtat cttttactag attaaattta ccagtcggta atccatcaga atcaacacat
     3301 gcatatttat cactatgcac taacaattga tgattataaa tatcttcttt agcccccgac
     3361 aaattaaaat acgaatgatt agtaatatta ataattgtat ccttatcact agttgcttga
     3421 taacgtacaa ttaaagtatc tttatttaaa gtgtaaattg ctttgaatgc taaattacca
     3481 ggatatccct cttcaccatc ttttgacaaa taagtaaact caattgaatc ctcaagtatt
     3541 tgataatcaa atatctgata tgaaaaacct ttgaccccac catgtaaac
//
LOCUS       DAOMTA010000232         3587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_935_length_3587_cov_3.057191, whole genome shotgun sequence.
ACCESSION   DAOMTA010000232 DAOMTA010000000
VERSION     DAOMTA010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_3587_cov_3.057191"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1686
                     /locus_tag="PLK68_07305"
     CDS             217..1686
                     /locus_tag="PLK68_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90254.1"
                     /translation="MNYPFKMYWGSASADFQYEGGFNEGKRGLITHDIVTDGSYTTPR
                     RLTYRLPDGTLGSVAYRESFPEGAVGCIHDHVYYPSHNAVDFYHHYKEDIKLLAEMGL
                     SMMRFGICWTRIYPTGEEKTPNEEGLQFYEDVIDECLTYNIEPMITICHDELPLYLAN
                     KYDGWSNRIMIDLYEKLCNALFERFKGKVKYWLTFNELNVLQGFSHLGTRNSDAQTTW
                     QAIHHLFIASARAKILAKKIMPKAMLGAMYATSPSYPKTCHPDDQLAWMKQRRRLFYF
                     SDVMLRGYYPSFARSFWDEYKVTIRMEENDEEILKEGTLDFYSFSCYRSTTIGKDDKL
                     GIIALPFGENPYLKSTPWGWPIDPVSIRYVLNEVYDRYQKPIFIVENGLGEVDKPDEN
                     NFVNDTYRINYLNDHFLEIKKAVEIDRVPVLGYTMWGGIDLVSLSTGEMKKRYGWVYV
                     DMDDKGNGSKKRYPKASFYWMKEFIKSNGNILKENNQGE"
     gene            1689..3474
                     /locus_tag="PLK68_07310"
                     /pseudo
     CDS             1689..3474
                     /locus_tag="PLK68_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
BASE COUNT         1237 a          492 c          698 g         1160 t
ORIGIN      
        1 agagtgagga tataatagta tgacgatcaa gcgctcaata gctaaggact tgatcaacat
       61 attcttatct cttttttaat aaatgacaaa tagttattaa aggtaagctt ttgtttcatg
      121 ttttgaaaca gtgtgccagc ttgtgaaaaa tcacagatta agtgattttt tttattatac
      181 taacagtgtg ataagacagg tacaggagga caaaatatga attatccatt taaaatgtat
      241 tggggaagtg ccagtgcaga ctttcaatat gaaggtggat ttaatgaagg aaaaagagga
      301 ctcattacac atgacattgt gactgatggt tcgtacacaa cccctagaag gcttacatac
      361 agattgccgg atggaacgtt aggttcagtt gcatatagag aatcatttcc agaaggtgca
      421 gtagggtgta ttcacgatca tgtttattat cctagtcata atgcggttga tttttatcat
      481 cactataaag aggatattaa gttattagca gaaatgggat tatcaatgat gagatttgga
      541 atctgctgga caagaattta tcctactggt gaggaaaaaa ctccgaatga agaaggttta
      601 cagttttatg aagatgtcat tgatgaatgt ttaacataca atattgaacc aatgattaca
      661 atttgccatg atgagcttcc tttatatctt gctaataaat atgatggctg gtctaatcga
      721 ataatgattg atttgtatga aaagttatgt aatgcgttat ttgaaaggtt taaaggaaaa
      781 gtcaaatact ggcttacgtt taatgaactt aatgtacttc aaggattctc gcatctgggt
      841 acaagaaatt cagatgctca aactacttgg caggcaattc atcatttgtt tatcgccagt
      901 gccagggcta aaattttagc aaaaaaaatt atgccaaaag caatgttagg agcaatgtat
      961 gcaactagtc catcgtatcc taaaacgtgt catcctgatg atcaacttgc atggatgaaa
     1021 caaaggagaa gactattcta tttttcagat gtaatgctta gaggttatta tccaagcttt
     1081 gcacgaagtt tctgggatga atataaggtg actataagaa tggaagaaaa tgatgaagaa
     1141 atcttaaaag aaggaacctt agacttttat tcatttagct gttatagatc aacaacgatt
     1201 gggaaagacg ataaattagg aatcatcgca ttaccattcg gtgaaaatcc atatttaaaa
     1261 tctacaccct ggggatggcc tattgatcca gttagtatac gatatgtgtt aaatgaagtg
     1321 tatgatcgtt atcaaaaacc aatttttatt gtcgaaaatg gtttgggcga agtcgataaa
     1381 ccggatgaaa ataattttgt aaacgataca tatagaatca attatctaaa tgaccatttc
     1441 ttagaaataa aaaaggcggt agaaattgat agggtaccag ttttaggata taccatgtgg
     1501 ggaggcatcg atctagtatc actaagcact ggtgagatga aaaaacgtta tggctgggtc
     1561 tatgtggata tggacgataa aggaaatggc tctaaaaaga gatatcccaa agcatcattt
     1621 tactggatga aagagtttat taaatcaaat ggaaatattt taaaagaaaa taatcagggg
     1681 gaatgatcat gacttataca gaattaggaa aacaaatcat tgcgtatgta ggtggtaaag
     1741 ataatgtaaa aaatatcatg cactgtatga ctcgcttacg catgaattta aaagataata
     1801 ctaaagtaga aattgaaaaa ctagaggctt taaaagatgt attaaaagtg cagtttaaaa
     1861 atgaacaatt acaggtagta attggaccac aggtaagtga aatttatcag acgattgaaa
     1921 aagatttcaa tttttctgaa gcagataatt tagaagaaaa agagaaacaa ggaattgtca
     1981 aaagcttttt aaatatatta tcaagtgtat ttgtgccagt tattcctgca attgctagtg
     2041 ctggtatgtt gaaagcaatc attgcattaa ttaaagcatt tgagattatt cctcaagata
     2101 atggtgtctt tatcgtattt aatatgatgg cagatgttgc attttatttc ttaccaatct
     2161 tgttggcagc atcagcatca aagattttta atacgaatcg aatgatttca attgtattag
     2221 cagctactct cattcatccg acttttacaa ctcttgttgc tgatacagaa gcatcattat
     2281 cattctttgg tttaccagta cctttgatta attatgctag ttcagttgta ccggtaattt
     2341 tgtcagtatg gattttatct tatatatatc gttatgtaga taaaataatg ccaaatgcac
     2401 taaaagtaat ttttactcca acaatttcac ttttaattat ggttccatta atgctggttg
     2461 tgttagggcc tctaggtaac tatgttggtg tacttatttc atattgtgtg ggaggactat
     2521 ttacatttaa tcgctttatt gggggattta tcttatcatt cattagacca ttgttagtaa
     2581 ttacaggtat gcatcaggca tttacccctg ttatttttca aaatttggca gaacgcggag
     2641 gagatttctt gcttcctaca atgatgatgt ctacaatggg tcaatttggt gcagttgccg
     2701 caatgatttt taaaacaaga aataaagaaa aaagaacaat tagaacttct gcaagtatat
     2761 cagctatctt aggaatcaca gaaccagctt tatatacagt tcttatccac aatagaaaag
     2821 cattaatatc agcttgttta ggtggagcac tgggtggtgc atttatttcg atgaccggtt
     2881 ttgaactgcc agcatttgct tctagtagta ttgttagttt accaatttat ttacaggtaa
     2941 atgttacaaa tgtaattatt gctttcttga ttagtattat atcgtctttt gttattgcaa
     3001 tgcttcttgt gaaaactgaa aaagacgaga ttgttgaaac aaatggtata atttctccaa
     3061 tagcaggaaa attaatttca ctaagtgatg ttaatgatga aactttttca aaggaagtaa
     3121 tgggaaaggg gtttgcaatt attccggcag aaggaaaagt tattgcaccg tttaatggta
     3181 cagtaaacgc agtatttcca actaatcatg caatcggaat caggagtaga gcttttaatt
     3241 catgtaggta ttgatactgt ggaattaggg ggaaagcatt ttgaagcaat ggtaaaacaa
     3301 ggcgcaaaag ttaataaagg tgatgtcctg ctagcatttg atttaaatga aattagtaaa
     3361 cattacgatt caactacctc agtaatcttt ttaaataaac cggatgctcg cttaaattcg
     3421 gatattaata aggatgttat gttaggccaa acactaaata ttgattttaa ctaaaactaa
     3481 ataaacacat ctagataaaa agcaggtaaa gagttgttct gaactgctcc ctcctaggtg
     3541 acaagtcatt aatttacttg tctacttaga agggagcata tcattca
//
LOCUS       DAOMTA010000233         3581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_939_length_3581_cov_2.647476, whole genome shotgun sequence.
ACCESSION   DAOMTA010000233 DAOMTA010000000
VERSION     DAOMTA010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_3581_cov_2.647476"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLK68_07315"
     CDS             complement(<1..207)
                     /locus_tag="PLK68_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRM90255.1"
                     /translation="MNQEKIMQLEEIINTKYDNITGIIGLKNGVTKYENYFNGGNVNS
                     TVHVASVTKSIISILIGIALDKGYL"
     gene            complement(228..1055)
                     /locus_tag="PLK68_07320"
     CDS             complement(228..1055)
                     /locus_tag="PLK68_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM90256.1"
                     /translation="MLSIGEFSKICQVSTKTLRYYAEIGLILPDEINLENGYRYYSID
                     QLETMLFINRLKSYNFSLEEIKEILETEEAQDEKLCKALMKKKKEIDLKIQNYQNSLA
                     QLHEDITTLQQGKSIMSYLDKIDVQLVEIPPMNLLNIRKMIQEDEFAEAYSNSFGQLF
                     REIMTDKLTVTAPPMVLFHSSEFTLQGLDTEFAIPVQETNNRTREFVPGTCLKTVVHG
                     SYSNLSSVYIKQCEWAEKIGYKNDAPLYEVYVNDPSQVTSDKELITEVYYPVSKLKV"
     gene            complement(1161..2630)
                     /locus_tag="PLK68_07325"
     CDS             complement(1161..2630)
                     /locus_tag="PLK68_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90257.1"
                     /translation="MRKFPEGFLWGGATAANQVEGGWKEGGKGISVSDCARHHLDVDV
                     ENYKAHNHITSKDIEEALASDDDSLYPKRHGSQFYYHYKEDIKMFAEMGFKVYRMSIA
                     WSRIFPNGDDEQPNEAGLKFYDDVFDECAKYGIEPLVTMSHYEPPLNLVLKYNGWYNR
                     KLVGFFETFVKTICERYKNKVKYWLTFNEVDSMIRHPYTTGGLIEDRFPGIKWNQVIF
                     QAMHHQFVASALATKICHEIISDSMVGCMLTKLTYYPYSCKPEDVLQAQQDMRGTYCY
                     SDTQVFGEYPAYLLAKFKNEGVEIVKEPGDDEVMKKYPVDFVSFSYYMSSCSAASSEG
                     LDTAVGNTVTAVKNPYLPSSEWGWQIDPIGLRISMVDLYDRYRKPLFIVENGLGAKDV
                     VLPDGTIDDQYRIDYFDCHFKEMLNAIEIDGVECLGYTSWGCIDIVSESTKQMSKRYG
                     FIYVDADDYGKGTYKRIPKKSFYWYKKVIETNGACLFED"
     gene            complement(2756..>3581)
                     /locus_tag="PLK68_07330"
     CDS             complement(2756..>3581)
                     /locus_tag="PLK68_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90258.1"
                     /translation="ENLTNQEKAVVDYIIANPKALLEMSVNELANASYTSASTITRLC
                     KKLGTKGYADMKFIYVSEYPEMMKLKDSLKVVPFSRNSGIDDIIHTMPLIYSRAIDHT
                     RSMLDRNTVIRVSNLMKRAQVIDLYGDGINFEIARNICYKLDEIGISANAYNSIQWNH
                     CKRLQQDKIPSFSILLSHTGKNPSMVDAAKRLKQYNIPSLSITGNVDKRLLNLTDYNF
                     QIMITENTFEFSTVIFTMSSLYILDILVASLIVHNYDKIERHLEELIGQRYDWQND"
BASE COUNT         1162 a          680 c          450 g         1289 t
ORIGIN      
        1 tagataacct ttatctaaag ctatcccgat taaaattgaa ataatacttt tagtaacaga
       61 agcaacatgt accgtactat ttacgttacc cccattaaaa taattttcat attttgttac
      121 accatttttt aatccaataa ttcctgtaat attatcatat tttgtattaa tgatttcttc
      181 taactgcatt attttctctt ggttcatcta gttctccttt attttcatca aactttaagc
      241 ttacttactg gataataaac ttcagtaata agttctttgt cacttgtaac ctgtgatgga
      301 tcatttacat atacttcata caaaggggca tcatttttat aacctatttt ttccgcccat
      361 tcacattgtt taatatatac cgaagacaaa ttagaataag agccatgaac aaccgtttta
      421 agacatgttc ccggtacaaa ttctctagtt ctattatttg tttcttgtac aggaatggca
      481 aattctgtat ctaacccttg taaagtaaat tctgagctgt ggaaaagtac catcggtggc
      541 gcggttactg ttaacttatc ggtcataatt tctctaaaca attgtccaaa actattactg
      601 tatgcttcag caaattcatc ttcttgaatc attttacgaa tattcagcaa attcataggc
      661 ggtatttcta ctagctgcac atcaatttta tctagatacg acataattga ttttccttgt
      721 tggagagttg tgatatcctc atgcagctgc gctaaagaat tttgataatt ttggattttt
      781 aaatcaatct cctttttctt tttcattaaa gctttacata atttttcatc ctgtgcttct
      841 tctgtttcaa gaatttcttt aatttcttct agtgaaaaat tatatgattt taagcgatta
      901 ataaataaca ttgtttctaa ctgatcaata gaataatatc gataaccatt ttccagattg
      961 atctcatcgg gtaaaattag cccaatttca gcataatatc gtaatgtttt agtcgaaact
     1021 tggcatatct ttgaaaactc cccaattgat aacataaatt agtacctcct actaatctct
     1081 atgttcatct tacaccttgc cctaagggga aagtcaatgt aatctaatta agttttttat
     1141 aatccacaaa aaagaggacc ttagtcctca aataaacatg ctccattagt ttcaattacc
     1201 tttttatacc aataaaaact ttttttagga attcgtttgt atgttccctt accgtaatca
     1261 tcagcatcta cataaataaa gccatatctt ttactcattt gctttgtcga ttctgaaaca
     1321 atatcaatac atccccaaga agtatatcct aaacattcga ctccatcaat ttcaatagca
     1381 ttaagcattt ctttaaaatg acaatcgaaa taatcaatcc gatattggtc atcaatagtt
     1441 ccatcaggta aaactacatc ctttgcaccc aaaccatttt ctacaataaa taacggttta
     1501 cgataacggt catataaatc aaccattgaa attcttaaac caattggatc aatttgccag
     1561 ccccattcac ttgaaggcaa gtaaggattt ttaactgctg tgacagtatt tccaactgct
     1621 gtatctaaac cttcgctact agcagctgaa catgatgaca tatagtaact aaatgataca
     1681 aaatcaacag gatacttttt catcacttca tcatcaccag gttcttttac aatttctact
     1741 ccctcatttt taaattttgc taataaataa gcaggatatt caccaaatac ttgagtatca
     1801 ctataacaat atgtacctct catatcttgt tgggcttgta agacatcttc tggtttgcaa
     1861 ctataagggt agtatgtcag tttagtcaac atacacccta ccattgaatc agaaataatt
     1921 tcatgacaaa tttttgttgc taacgcacta gctacaaatt gatggtgcat tgcttgaaaa
     1981 attacctgat tccatttaat tcctggaaaa cgatcttcaa tcaaaccacc agtagtataa
     2041 ggatgacgaa tcatactatc cacttcatta aaagtaagcc aatatttaac tttattttta
     2101 taacgttcac aaattgtttt tacgaacgtt tcaaagaatc caactaattt acgattatac
     2161 cagccattat attttaatac taaatttaat gggggttcat aatgtgacat tgttacaagt
     2221 ggttcaattc catactttgc acactcatca aatacatcat cataaaattt taagcccgct
     2281 tcatttggtt gttcatcatc accattaggg aaaattcgcg accaggcaat cgacatccgg
     2341 taaactttaa accccatttc tgcaaacatt ttaatatctt ctttataatg ataataaaac
     2401 tgactcccat gccgttttgg atacaaacta tcgtcatcac ttgctaatgc ttcttcaata
     2461 tctttactag taatatgatt atgagcttta taattttcaa catcaacatc aaggtgatga
     2521 cgggcacaat cagaaaccga aatacctttt ccaccttctt tccagccacc ttctacttga
     2581 ttagctgcag ttgcaccgcc ccataaaaaa ccttctggaa attttctcat cataactcct
     2641 cctatataat tttattataa gtttttaatc agcagttgcc catatcgata tattgtttcg
     2701 gttagaatta tcaaatatta taatttgttt taagcttttt tgaaattatc aaccattagt
     2761 cattttgcca atcatagcgc tgcccaatca attcttcaag atgccgttct attttatcgt
     2821 aattatgaac aattaaagat gctactaaga tatctaaaat atatagtgat gacatagtaa
     2881 aaattactgt tgaaaattca aaggtatttt ctgttatcat aatttgaaaa ttataatctg
     2941 ttagattcaa taaccgctta tcaacattcc cggttattga taaactaggg atattatatt
     3001 gttttaaacg tttcgcagca tcaaccatac tcggattttt accagtatgt gataataaga
     3061 ttgaaaatga aggaatttta tcttgctgca atcttttaca gtgattccac tggatactgt
     3121 tataagcatt tgctgaaatt ccgatttcgt caagtttgta acaaatatta cgagcaattt
     3181 caaaattgat tccatcaccg tataaatcaa ttacttgtgc tcgcttcatt aagttgctta
     3241 cgcgaataac cgtattacga tcgagcatgc tgcgagtatg atcaatagca cgtgaataaa
     3301 ttaacggcat agtatgaata atatcgtcaa ttccactatt tcgactgaat ggtactactt
     3361 ttaatgaatc ctttaatttc atcatttcag gatattctga tacataaata aatttcatat
     3421 cagcatatcc tttagtacct aattttttac ataatcttgt aattgtcgat gcactagtat
     3481 aacttgcatt agctaactca ttaacactca tttccaataa agctttagga ttagcaataa
     3541 tgtagtcgac aactgctttt tcttgattag taagattttc t
//
LOCUS       DAOMTA010000234         3569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_947_length_3569_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOMTA010000234 DAOMTA010000000
VERSION     DAOMTA010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_3569_cov_3.000000"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLK68_07335"
     CDS             <1..1341
                     /locus_tag="PLK68_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90259.1"
                     /translation="GATYFYQNEKCYDPISQEAWMSHNGTVNGYAAYKVANDVKEHYA
                     VGLGIYNVFIYTGEDYDSSKVQIQMDSAIEVPNSKGVIIENACLQTFADENKVLQKFN
                     HIINDVGPGVSSGTDKDDFAIKGEGWSRKFLLNYCDGTAVYGKMPAADQKGKFIGTVT
                     EENIKALGDDNIDTKTLSDLYNVNKDKNENNYTVDSWKVFVKALDDAGKQLNADLKYA
                     YQKDFDSAKDVLNDAIDNLVLVGADYSKVDSIKAEAEKVLASDNYTKDYYTEATRATF
                     DKANKALNDLADDLTILEQNKVDDAIELLTEAINGLTLKGANYSKLDVVKKEAEKVLI
                     SDNYTKGHYTEESKALFDEAYEVLSSLADNLTIIDQGMVDEVVKALDTAIKGLTLKTE
                     EPGQIVKPNEPGQSTKPSDSSSAKTGDDQMIYGYGIVTLLALGGLMMLKRHHTS"
     gene            1354..>3213
                     /locus_tag="PLK68_07340"
     CDS             1354..>3213
                     /locus_tag="PLK68_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRM90260.1"
                     /translation="MKKSKIIKVAILALFSAVLLTLNNVGAISSNPYNDWKTSAINYP
                     NNGQLVPAGPITITWDRLSIDSHEVTGYEVYLDNVLQNSTIIDEGDIFSCEVYTTKVA
                     QHQVKILAQLSNNTKISTSARNFYISKKGMGFYSGNGYSAIQDAQNMGLSWYYNWGTA
                     PTYAGTCPNQKIDFVPMIWGAYNGSNEQLTTIKNAGYKTVLGYNEPDFVDQSNVPVAT
                     AIANQHYFTNSGMRIGAPATAIQAPNSEWFNEYWQGINTDDIDFIPVHNYPGNIGVTD
                     KEIKDNAKSFLNFINETHNKFNKPIWVTEFAVANWDPYWDGYNGANEANKAEVRKFLN
                     YVINGFDNNVGLNDLEFVERYAWFSFDALDRYGGDSGLFNTKADHDKNSMLKIGTLTT
                     LGNDYRNLGNPEGYILPNLMGEIEPSIEDEYVDDYVNVMINGRSENVVLGSKFDKIDT
                     PVKDGYVFSGWYSDEACTKEYDFDSIVTDNVTIYPKWLKMHTVTIDGIDNKVIDGNTA
                     VKPLPSVKDGYVFKGWYSDSEYNNKFDFNEPIRGDIAIYSKWVKLVTVSVDNNKVLIE
                     SGTVLSRPDMPIKNGYTFAGWYSDEACTKEFDFAKPLLEDVTIYTKWKKVIDPM"
     assembly_gap    3214..3223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1307 a          384 c          731 g         1137 t
ORIGIN      
        1 ggtgcaacat atttttatca aaatgaaaaa tgttatgatc ctattagtca agaggcttgg
       61 atgtcacata atggaacagt aaatggatac gctgcttata aagtagctaa tgatgttaag
      121 gagcattatg cagttggatt gggaatttat aatgtattta tttatactgg tgaagattat
      181 gattcttcaa aagtgcaaat tcaaatggat agtgcaattg aagtaccaaa tagcaaaggt
      241 gtaattattg aaaatgcatg tttacaaaca tttgctgatg aaaataaagt attacaaaaa
      301 tttaatcata ttattaatga tgttggtcct ggtgtaagca gtggaactga taaagatgat
      361 tttgctatta aaggtgaagg ctggtcaaga aaattcttat taaattattg tgatggaact
      421 gctgtttatg gtaagatgcc agctgctgat caaaaaggaa aatttattgg aacagtaact
      481 gaagaaaaca taaaagcatt aggtgatgat aatattgata caaaaacatt gagtgatcta
      541 tataatgtga ataaggataa aaatgaaaat aattatacag ttgatagctg gaaggtattt
      601 gtgaaagcgt tagatgatgc tggtaaacaa ttgaatgctg atttaaaata tgcatatcaa
      661 aaagattttg atagtgcaaa agatgtattg aatgatgcta ttgataattt agtattagtt
      721 ggtgctgatt atagtaaagt tgatagtatc aaggctgaag ctgaaaaagt tttagcaagt
      781 gataactata ctaaagatta ttacacagag gcaacaagag cgacatttga taaagctaat
      841 aaagcattaa atgatcttgc tgatgattta acgattttag aacagaataa agttgatgat
      901 gcaattgaat tattgactga agcaattaat ggtttaacat taaaaggtgc caattatagt
      961 aaattagatg tagttaaaaa ggaagctgaa aaagttttaa taagtgataa ttatactaaa
     1021 ggtcactata cagaagaatc gaaagcattg tttgatgagg cttatgaagt gttaagtagt
     1081 cttgctgata atttaacaat tattgatcaa ggtatggttg atgaagtagt aaaggcatta
     1141 gatactgcga ttaaaggttt aacattaaaa actgaagaac cggggcaaat tgtaaaacca
     1201 aatgagcctg gtcaatcaac aaaaccaagt gatagcagta gtgctaaaac aggcgatgat
     1261 caaatgattt atggttatgg aattgttact ctccttgcac tgggtggttt aatgatgtta
     1321 aaaagacatc atactagcta gtggagaaga attatgaaaa aaagtaaaat tataaaggta
     1381 gcaatactcg cactatttag tgcggtattg ctcacattaa ataatgtagg agctatttca
     1441 agtaatccat ataatgattg gaaaacttca gcgattaatt atcctaataa tgggcagtta
     1501 gttccagcgg gaccaattac aataacctgg gatagattaa gtattgatag tcatgaagta
     1561 actggttatg aggtatatct tgataatgtt cttcaaaatt caacaattat tgatgaaggt
     1621 gatattttta gttgtgaagt ttatacaact aaagtggcac agcatcaagt taaaatatta
     1681 gctcaattga gcaataatac taagatttca actagtgcta gaaattttta catatctaaa
     1741 aaaggtatgg gtttttatag tggtaacggc tacagtgcaa ttcaagatgc acaaaatatg
     1801 ggattgtctt ggtattacaa ttggggaacg gcaccaacgt atgctggtac ttgtccaaat
     1861 caaaagatag attttgttcc tatgatctgg ggtgcctata atggttcaaa cgagcaatta
     1921 acaacaatta aaaatgcggg atataaaaca gtattgggtt ataatgaacc ggattttgta
     1981 gatcaatcga atgttccagt agctacagcg attgcaaatc aacattattt tacaaatagt
     2041 ggtatgagaa ttggggcacc ggcaacggct attcaggctc ctaattctga atggtttaat
     2101 gagtattggc agggaattaa tacagatgat attgatttta tacctgttca taattatcct
     2161 ggtaatattg gagttactga taaagaaatt aaagacaatg ctaaaagttt tttaaatttt
     2221 attaatgaaa cacataataa atttaataaa ccgatttggg ttacagaatt tgcggtagct
     2281 aattgggatc catattggga tggttataat ggtgctaacg aagcgaataa agctgaagtg
     2341 agaaagtttt tgaattatgt tattaatggg ttcgataata atgtaggact aaatgattta
     2401 gaatttgttg agcgatatgc ctggttttca tttgatgcac tagatcgtta tggtggtgat
     2461 tcaggattat ttaatactaa agcagatcat gataaaaata gcatgttaaa aattgggaca
     2521 ttaacaactt taggaaatga ttatcgtaat cttggtaatc cagaaggtta tatattgccg
     2581 aacttaatgg gggaaataga accgtctatt gaagatgaat atgttgatga ttatgttaat
     2641 gtcatgatta atggtcgaag tgaaaacgtg gtattgggta gtaaatttga taaaatcgat
     2701 acgcctgtta aagatgggta tgtatttagc gggtggtata gtgatgaggc gtgtactaaa
     2761 gaatatgatt ttgatagtat tgtcactgat aatgtcacaa tttatccaaa atggcttaag
     2821 atgcacacag taacaattga tggtatagat aataaagtaa ttgatggcaa tacagcggtt
     2881 aagcctcttc cttctgttaa agatggttat gtatttaagg gatggtatag tgattcagaa
     2941 tataataata aatttgattt caatgagcca attagaggcg atattgcaat ttattcaaaa
     3001 tgggtaaaat tggtaacggt ttcggttgat aataataaag tgctgattga aagtggtaca
     3061 gttttatcaa ggccagatat gccaataaaa aatgggtata cttttgcagg gtggtacagt
     3121 gatgaagcgt gtactaaaga gtttgatttt gctaagccat tgttagaaga tgtgacgatt
     3181 tatactaagt ggaaaaaagt aattgatcca atgnnnnnnn nnngaaaaag aagaccaaac
     3241 cacttcaatt aatagtgtta aaaccggcga taattttgca attcaaggtt atgtcctagg
     3301 tttgattgga gtaatggcgg caattgtaat gatgcaaaaa aaatataata aataaagata
     3361 ttatgtatat aattgactac ttgataaaaa acctctcctc attgaaattt atcaaattaa
     3421 atacaagtag ttcaagatgt taaaagggaa gtgaaggcca cttccttttt tgtgaaaaaa
     3481 acagtattta ctattgtttg aatattgttt ataaatcaac gattatgcaa agagttcttt
     3541 caaaattatt atcaattatt ttagtattt
//
LOCUS       DAOMTA010000235         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_951_length_3567_cov_2.838269, whole genome shotgun sequence.
ACCESSION   DAOMTA010000235 DAOMTA010000000
VERSION     DAOMTA010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_951_length_3567_cov_2.838269"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..2019
                     /locus_tag="PLK68_07345"
     CDS             94..2019
                     /locus_tag="PLK68_07345"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRM90261.1"
                     /translation="MGMNLAYKILSSKLKDGNLVPGEQIGIQIDQTLTQDSTGTMAYL
                     QLEAMNIKHVAVEKAVAYIDHNMLQTGFENMDDHEFIRSVAKKHGIVFSKPGNGVCHQ
                     LQLENFSKPGKTLVGSDSHTPTCGAMGMIAIGAGGLDVAVAMATGKYYLQCPSVVKVN
                     LTGKKAPWVSAKDIILYILQQLTVKGGVNKIIEYTGDGVASLSLTDRATICNMGAELG
                     ATTSLFPTDERTKEYLAQQGRVDDYIEMKADEDATYDQELDVDLSALVPMTAKPHSPD
                     AVVPVKELEGMKVNQVVIGSCTNSSFADMMKAAKILKGRKVADHVSLVIAPGSSSILA
                     MLSQNGALADMVQAGARILECGCGPCIGMGQAPLSKGISLRTINRNFKGRSGTNDASV
                     YLVSPEIAALSAIKGYMSEEFEDDMYLEEVPNTPFIKNGNFFIDEYDENNEVYMGPNI
                     KPVPRGEKITDEISGKVVLKVGDNISTDHIVPSDSKLLPYRSNVPHLAKFSFSKVDPE
                     FYDRAIANNGGFIVGGDNYGQGSSREHAALVPNYLKIKAIFAVSFARIHRSNLINNGI
                     LPLVIEAKDQDFFNDQDSYKIVNIKDVVEHNGKVKVINETTNDSIEAELTLSPREKVM
                     INYGGLLNAIKELGGEF"
     gene            2019..3014
                     /locus_tag="PLK68_07350"
     CDS             2019..3014
                     /locus_tag="PLK68_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HRM90262.1"
                     /translation="MKAVLIPGDGIGKEISKSVVDITKAMKLDIEWMEYQAGAEYAAT
                     TKEVFEPGLVDAIKEYKWALKGPTATPIGTGFRSVNVALRQQFATYANVRPIKSFKGI
                     NSRYEDIDLVMIRENTEDLYKGIEYMINPNMANGIKLITREASEKICRYAFEYAKNNR
                     RKKVTAVHKANIMKYTDGLFLEAFRDVAKDYPDIEPQEVIVDNMCMQLVIRPETFDVL
                     VAPNLYGDIVSDLCAGLVGGLGFAPSGNIGDEFRIYEAVHGSAPDIAGKGIANPSALL
                     LAFALMLEALGKLDDANKLREALAAVVEEGTIVTPDIGGSASTDEFTAAIIRKLG"
     gene            3018..>3567
                     /locus_tag="PLK68_07355"
     CDS             3018..>3567
                     /locus_tag="PLK68_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM90263.1"
                     /translation="MTKNLLENTGHGSLGNKIFTVLRDKILNEEYTQGQKLNEVALSK
                     ELNISRTPIREALKQLELEGLVKSIPNKGVYVLGFSHRDIDDMLEIRYALEGLAIQLA
                     IERINDEELEKIKEVYDLMEFYAEKGDQEKFNEINIAFHEAIYRCTQSKYFEQLLTDI
                     NYYIHVTSRHSIRQPDRLISAAQ"
BASE COUNT         1231 a          468 c          769 g         1099 t
ORIGIN      
        1 catcttgtgg taaaatttgt tccaaagggt tgtaatcaag attgtttttt tgtataattg
       61 tatgcaatat aagaataatg ggggtatatc gtgatgggta tgaatttagc atacaaaatt
      121 ttaagtagta aacttaaaga tggaaatctt gttccaggag aacaaattgg aattcaaatt
      181 gatcaaacac taacacaaga ttcaacaggg acaatggctt atcttcaact agaagcaatg
      241 aatattaaac atgttgcagt agagaaagca gtggcatata ttgatcacaa catgttacaa
      301 acaggatttg aaaatatgga tgatcatgaa tttatccgca gtgtggctaa aaagcatggg
      361 attgtttttt caaaaccagg taatggagtt tgtcatcaat tacaattaga aaacttctca
      421 aaaccaggaa agactctagt tggatcagat tcacatacac caacttgtgg agcaatgggt
      481 atgattgcta ttggggcagg gggattagat gtcgcagtag cgatggcaac aggtaaatat
      541 tacttacaat gtcctagtgt tgttaaagtt aatttgactg gaaaaaaagc accatgggta
      601 agtgcaaaag acattatttt atatattctt cagcaattaa cggttaaggg tggcgttaat
      661 aagattattg aatatacggg agatggagtt gcttcattat cattgactga tcgtgctacg
      721 atttgtaata tgggggctga attgggagcc actacatcac ttttcccgac cgatgaaaga
      781 acaaaagaat atttagcaca acaagggcga gtagatgatt acattgaaat gaaagctgat
      841 gaagatgcta catatgatca agaattagat gttgatttaa gtgcattagt gccaatgact
      901 gctaagcctc atagtccaga tgcggtagta ccagtaaaag aacttgaagg aatgaaagta
      961 aatcaagttg tgataggaag ttgtactaac tcatcttttg ctgacatgat gaaggcagct
     1021 aagattttaa aaggacgcaa agttgcggat catgtttctt tagttattgc gccaggatca
     1081 agttcaattc ttgcgatgct ttcacaaaat ggagcattag ctgatatggt tcaagctgga
     1141 gcaaggattc ttgaatgtgg ttgtggaccg tgtattggaa tgggtcaagc accgctctca
     1201 aaagggattt cattaagaac gattaatcgt aatttcaagg gacgttcagg aacaaatgat
     1261 gcaagcgtat acttagtatc accggaaatt gcggcattga gcgcaattaa agggtacatg
     1321 tcagaagaat ttgaagatga tatgtattta gaagaagtgc caaatacccc atttattaaa
     1381 aatggaaatt tcttcattga tgaatatgac gaaaataatg aagtatatat gggacctaat
     1441 attaaaccgg ttccacgtgg agaaaaaatt acagatgaaa ttagtggaaa agttgttcta
     1501 aaagttggtg ataatatttc gacagatcat attgttcctt cagatagcaa attattgcca
     1561 tatcgttcaa atgtgccaca tttagcaaaa ttttcattca gtaaagtaga tcctgaattc
     1621 tatgatcgtg caattgctaa taatggggga ttcatcgttg gtggtgataa ttatggtcaa
     1681 ggaagttccc gtgaacatgc ggctttagta ccaaactatt taaagattaa agcaatcttt
     1741 gcagtatcat ttgcgagaat tcatcgttct aatttaatta ataatggtat tttaccttta
     1801 gttattgaag ctaaagacca agatttcttc aatgaccaag atagttataa aatcgttaat
     1861 atcaaagatg ttgttgaaca taatggtaaa gtaaaagtaa ttaatgaaac tactaatgat
     1921 tcaatcgagg ctgaattaac attatcacct cgtgaaaaag taatgattaa ttacggtggt
     1981 ttattaaatg caattaaaga attgggaggt gaattttaat gaaagctgtt ttaatccctg
     2041 gtgatggaat tggaaaagaa atatcaaaga gtgtcgttga tatcactaaa gcgatgaagc
     2101 ttgacattga atggatggaa tatcaggctg gtgctgaata tgcagctaca acgaaagaag
     2161 tttttgaacc aggacttgtt gatgctatta aagaatataa atgggcttta aaaggtccga
     2221 cagcaacgcc gattggaacg ggatttagaa gtgtcaatgt tgccttgcga caacagtttg
     2281 caacttatgc taatgttcgt ccaatcaaat catttaaagg aatcaattct cgttatgaag
     2341 acattgatct ggttatgatt cgtgaaaata ccgaagattt atataaaggg attgagtata
     2401 tgatcaatcc aaatatggca aatggaatta aattaattac tcgtgaagcg agtgaaaaga
     2461 tttgtcgtta tgcttttgaa tatgctaaga acaatagacg taaaaaagtg acagcagttc
     2521 ataaagcgaa tattatgaaa tatacagatg gattattttt agaagcgttt agagatgtgg
     2581 ctaaagatta tccagatatt gagccacaag aggttattgt tgataacatg tgtatgcaat
     2641 tagtaattcg tccagaaact tttgatgttt tagttgcacc aaatttatat ggtgatattg
     2701 tttctgattt atgtgctgga ttagttggcg gtctaggatt tgcaccaagt gggaatattg
     2761 gtgatgaatt tagaatttat gaagctgttc atggaagtgc accagatatt gctggtaagg
     2821 gtatcgctaa tccgagtgca ttattattag catttgcatt aatgttagag gcattaggga
     2881 aattagatga tgctaataaa ttgcgtgagg ctttagcagc tgtagttgaa gaaggaacta
     2941 ttgtaacacc tgatattggg ggtagtgctt caacagacga gttcaccgca gccatcatca
     3001 gaaagttagg ttaaacaatg acaaagaatt tattagaaaa taccggacat gggtcgcttg
     3061 gaaataaaat tttcaccgtt ttgcgtgata aaatcttaaa tgaggaatat actcaaggac
     3121 aaaaattaaa tgaagttgct ttgtctaaag agttgaatat ttctagaact ccaattcgtg
     3181 aggctctaaa acagttggaa ttagaaggtt tggttaaaag tattcctaat aaaggagttt
     3241 atgtactagg gttttctcat cgtgatatcg atgatatgtt ggaaattcgt tatgctttag
     3301 aagggctagc aatccaatta gcaattgagc gaattaatga tgaggaactt gaaaagataa
     3361 aagaagtgta tgatttaatg gaattttatg ctgaaaaagg tgatcaagag aagtttaatg
     3421 aaattaatat tgcttttcat gaggctattt atcgttgtac ccaatcaaag tattttgagc
     3481 aattattaac agatattaat tattatattc atgtaacttc aaggcattca attcgtcaac
     3541 cggatcgttt aatttcagca gcacagg
//
LOCUS       DAOMTA010000236         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_954_length_3555_cov_2.067714, whole genome shotgun sequence.
ACCESSION   DAOMTA010000236 DAOMTA010000000
VERSION     DAOMTA010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_3555_cov_2.067714"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PLK68_07360"
     CDS             complement(<1..1114)
                     /locus_tag="PLK68_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM90264.1"
                     /translation="MLDDFLIHVFHKGNTLEAYKVFGAHLETNDHKKGVRFTVYAPNA
                     RSVQVVGDFNDWDGQNHYMERYTDGGIWTLFIPGVKEFALYKYRIETQNFATVDRADP
                     YAFYSELRPGTASRVYNIERFRWADKKWLNSRTKNFDKPMNIYEVNIGSWKMKKDFTA
                     EEDGEFYSYEEMIELLIPYLVENNYTHLELMPLTEFPFDGSWGYQATGYFSITSRYGE
                     PKGLMKFINACHKAGIGVIMDFVPAHYVKDGHGLYKFDGGFVYDYPDINRRYTEWDSV
                     YFDVGREEVRSFLMSSVGFLAEYFHIDGIRFDAVSNLIYWKGNKEAGVNDGALEFMKR
                     MNNHMKGYYPGVMLIAEDSSDFNGVTKAPDIGGLGFD"
     gene            complement(1323..2327)
                     /locus_tag="PLK68_07365"
     CDS             complement(1323..2327)
                     /locus_tag="PLK68_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM90265.1"
                     /translation="MATLKEVAEFANVSIATVSRILNDDNSLTVLPETKEKVIKAAEQ
                     LNYIPKSKRKGNHDEALTVAVIQWYSFEQEMRDTYYLSLIQGVENYLKTNGIIAKRIR
                     KDDMDFNKVLENVQGIICIGKFEFEVVKELKEYTENIVLLDMDISPVTECCVTLDFDN
                     AMFQVVDYFNRLGHKHIGFLGRGEYEELSLHISTRKSSFIKYCQYFGLKYTELLLENE
                     LTSEAGYNLIHNLAKTEELPTAIFAVNDPIAIGAMRALQDQGIRVPEQVSIIGFNNIE
                     AGNFTTPSLTTVYAPSKEMGNLGAMLLHEMILKKKALFPMRVQLPCSLLERKSCKKII
                     "
     gene            complement(2499..>3555)
                     /locus_tag="PLK68_07370"
     CDS             complement(2499..>3555)
                     /locus_tag="PLK68_07370"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRM90266.1"
                     /translation="PLMCMEFWDGWFNRWNQEIIKRDPQEFVNSAQEMLSLGSVNFYM
                     FQGGTNFGWMNGCSARKEHDLPQITSYDYDAILTEYGAKTEKYHLLREVITGKKERLP
                     ERRQTKNYGQIIKNRSVSLFSTLDCIAACHQSDWPLTMEELDHYYGYVVYQHTFKSYT
                     DDLRMRIIDGRDRAKIYLDDQEIATQYQEEIGDEINLPTHSNDTHDLKILMENMGRVN
                     YGSKLQAETQQKGIRNGVILDIHFTKKWKHYCLNFEHLDLLNWENGYQSGPGFHEYIF
                     EADEVKETFIDLEGFGKGVVFVNGHHCGRFYEAGPTLSLYIPGPFLKKGINQIIIFET
                     EGCYRDKIELIGQPKYL"
BASE COUNT         1164 a          751 c          441 g         1199 t
ORIGIN      
        1 aatcaaatcc aaggccacca atatctggtg cttttgtaac gccattaaaa tcacttgaat
       61 cttcagcaat caacatcaca ccaggataat aacccttcat atgattattc atacgtttca
      121 tgaattctaa cgcaccatca ttaactccag cttctttatt gcctttccaa tagatcaagt
      181 tacttaccgc atcaaaacgt atcccatcaa tgtggaaata ttcagctaaa aagccaacac
      241 tactcattaa aaagcttcta acttcttcac gcccaacatc aaaataaaca ctgtcccatt
      301 cagtataacg acgattaata tctggataat catatacaaa tccaccatca aacttataaa
      361 gaccatgtcc atcttttaca taatgtgcag gaacaaaatc cataatgaca cctattcccg
      421 ccttatgaca agcattgata aatttcatta aacctttcgg ttcaccataa cgactagtta
      481 tactaaaata cccagttgct tgataccccc acgaaccatc aaacggaaat tctgtcagtg
      541 gcattaattc caaatgagta tagttatttt caactaaata tggaattaga agctctatca
      601 tctcttcata agagtagaat tcaccgtctt cttctgccgt aaaatccttt ttcattttcc
      661 aagaaccaat atttacttca taaatattca ttggtttatc aaaattcttt gttcggctat
      721 taagccattt tttatcagcc caacgaaacc gttcaatatt gtaaaccctt gacgctgttc
      781 ctggtcgtaa ctcactataa aacgcatatg gatcagcacg atctactgtc gcaaaatttt
      841 gtgtttcaat ccggtactta taaagagcaa attcttttac cccaggaata aataaagtcc
      901 agattcctcc atctgtatac ctttccatat aatggttttg cccatcccag tcattaaaat
      961 cacctacgac ttgtacactt cgtgcattag gtgcatagac ggtgaagcgg acaccttttt
     1021 tgtggtcatt tgtttctaaa tgagcaccaa atactttata cgcttctaat gtatttcctt
     1081 tgtgaaatac gtgaattaaa aaatcatcga gcatgtgtca ctcccccttt tcttagatat
     1141 tcccattata acataattta tctggtattt cattaaaaag atagcgcttt cattatgcca
     1201 ataattgata atataatact aaattcaaca tttatttaag taaaataaaa aaagcggcct
     1261 aataatcaaa cattcttgat tttaagacca cagcagcaaa aactatccta ataacactaa
     1321 ttttatataa ttttcttaca tgatttacgt tctagtaacg agcatggcaa ctgtactcgc
     1381 atcggaaata aagctttttt ctttaaaatc atttcatgta acagcatcgc accaagattt
     1441 cccatctcct ttgaaggcgc ataaacagtc gttaatgatg gtgtagtaaa attaccagct
     1501 tcaatattat taaacccaat aatagaaact tgttcaggaa ctcttattcc ctgatcttgt
     1561 aatgcccgca ttgcacctat agcaattgga tcgttgacag caaaaatagc cgttggaagt
     1621 tcctcggttt tagctaaatt atgtatcaaa ttatagccag cttcactcgt taattcattt
     1681 tccagcagca attcagtata tttcaagcca aaatactggc aatatttaat aaaactactc
     1741 tttctagtac taatatgaag tgataattct tcgtactcgc cccgtcctaa aaaaccaata
     1801 tgtttatgac ctaaacggtt aaaataatca accacctgaa acattgcgtt atcaaaatcc
     1861 aaagttacac aacattcagt tacagggctg atatccatat ccaacaaaac aatattttca
     1921 gtgtactcct ttaattcttt taccacttca aattcaaact taccaataca gataattcct
     1981 tgaacatttt ccaaaacctt attaaaatcc atatcgtctt ttctaattct tttagcaatg
     2041 attccatttg tttttaaata attttcaaca ccttgaatca aagataaata atatgtatcg
     2101 cgcatttctt gttcaaaaga ataccactgg ataactgcca ctgtcaaagc ttcatcatga
     2161 tttcccttac gttttgattt aggaatatag tttaattgtt cagctgcttt gatgactttt
     2221 tctttcgttt ctggtaaaac agtcaatgaa ttgtcatcat ttaatattcg tgatacagtt
     2281 gcgattgaaa cattcgcaaa ttcagcaacc tcttttaatg tagccatatt aaccctcctc
     2341 aatcgataaa acagctcttt catagtatta taagtgaatt ctatcataat ttctagatat
     2401 tcttttaata atcatttctc attaagatta ttagtaaaaa aagtttaaat tcacttgtct
     2461 agaatttaaa cttcttttaa tcagaatact tctactgttt atagatattt tggctgacca
     2521 attaattcaa tcttgtcacg atagcagcct tctgtttcaa aaataataat ttgattaatc
     2581 ccttttttta aaaacggacc tggtatatat agtgatagtg ttggtccggc ctcatagaat
     2641 ctgccacaat gatgaccatt tacaaagacc acaccctttc caaagccttc caaatcaata
     2701 aaagtttctt taacttcatc agcttcaaaa atatattcat ggaatcctgg gcctgattga
     2761 taaccatttt cccagttcaa taaatcaaga tgctcaaaat taagacagta atgtttccat
     2821 tttttggtaa aatggatatc taaaattacc ccattacgga ttcctttttg ttgcgtctca
     2881 gcttgtaact tagaaccata attgacccga cccatatttt ccattaatat ctttaaatca
     2941 tgagtatcat tgctatgtgt cggtaaatta atctcatcac caatttcttc ttgatattga
     3001 gtagcaatct cttgatcatc cagatatatt tttgctcgat cacgtccatc aataattcgc
     3061 atccgtaaat catcagtata acttttaaaa gtatgttgat agaccacgta accataataa
     3121 tgatctaact cttccatcgt taaaggccaa tcagactgat gacaggcagc aatacaatca
     3181 agcgtactaa ataaagagac acttcgattt tttattattt gaccataatt tttagtttga
     3241 cgccgctctg gtaaacgctc tttttttcca gtgattactt ctctcaataa atggtatttc
     3301 tctgttttag caccatactc agttagaatc gcatcataat catatgaagt tatttggggc
     3361 aaatcatgtt cttttcgagc cgaacagcca ttcatccatc caaaatttgt cccgccttga
     3421 aacatataaa aattgacact gcccaacgac aacatttctt gcgctgaatt tacaaattct
     3481 tggggatctc ttttaattat ttcttgattc cagcgattaa accagccatc ccaaaattcc
     3541 atacacatta acggt
//
LOCUS       DAOMTA010000237         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_955_length_3555_cov_1.606286, whole genome shotgun sequence.
ACCESSION   DAOMTA010000237 DAOMTA010000000
VERSION     DAOMTA010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_3555_cov_1.606286"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLK68_07375"
     CDS             <1..198
                     /locus_tag="PLK68_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRM90267.1"
                     /translation="RNCGIRLLRDSVVVYEGKLGSLKRFQDDAKEVAAGFECGLSIEN
                     FNDIKEGDIVEGYIMEEVEVK"
     gene            208..555
                     /gene="rbfA"
                     /locus_tag="PLK68_07380"
     CDS             208..555
                     /gene="rbfA"
                     /locus_tag="PLK68_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRM90268.1"
                     /translation="MILKKDKMNGIIQRELSQIIQVEVRDPKIGFCTITAVDMTTDLS
                     IAKVYVTFLGKDFDTRKGMEALNRSKGFIRSLLAKRLTIRKVPELIFVNDTSLEYGNK
                     IEKIIDDLNHHDK"
     gene            1025..2164
                     /gene="nagA"
                     /locus_tag="PLK68_07385"
     CDS             1025..2164
                     /gene="nagA"
                     /locus_tag="PLK68_07385"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRM90269.1"
                     /translation="MKYCITNGKVILKNQVVDANVYVENTKITEISNRQPDDETVIDA
                     KGRYVSPGFIDVHTHGRGGSDTMYNTFEDLDTITSTAVKTGVTGILPTTMTMSKEDTY
                     AAIKNVGDNMDKVGGSKILGVHMEGPFFNTKYKGAQPEEFMIKPTAENYSSLVGEYGK
                     IVKKLSLAPELKDSDKLIEYLVKEGVVVSIGHTNATYDEAVVGIKAGATSGTHTYNAM
                     TPLTHRNPGVVGAIMEHDEVYAELILDGIHVSYPAAKVLLRAKGLDKVILITDSIEAS
                     GLEDGQYKLGNQAVFVKDNSARLEDGTLAGSILAMNNAVKNAYQHLGLSINEAVNLAS
                     YNPAKNLNLIDLGEIAVNKTADIIMFDEEINVDFVMIDGNVKIGG"
     gene            2166..3044
                     /locus_tag="PLK68_07390"
     CDS             2166..3044
                     /locus_tag="PLK68_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRM90270.1"
                     /translation="MITLKNEVLEVTLANKGAEIIKIVGQDDQINYMWRRDPIQWANS
                     APILFPIVGALQNNECRIDGKTYTMTQHGFSRHSEYEANQINDQEVVFTLISNEEIAK
                     QYPYLFKLDVTYKLDKNVLSCNCKVTNTDSQTIYFQIGGHPAFACPFMENESSNDYYL
                     EFSENETVNQKIIDVEHKGMSDITLPFLDNEKRFFVRQQLFDNDAIVLKDVKSKTLSL
                     KSLNHNKSLVFHMEGFNHLGIWAAKHVGGLIAIEPWVGHSDYVGFTGEFKDKEGVVSL
                     NPSEIFECTFKVEINQ"
     gene            3083..3256
                     /locus_tag="PLK68_07395"
     CDS             3083..3256
                     /locus_tag="PLK68_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HRM90271.1"
                     /translation="MKVKIIEKTHELDLEDEINDFLEKKNPKILSLHYQVAMTFSSEL
                     EYSFSCLIEYEDK"
     gene            3325..3474
                     /locus_tag="PLK68_07400"
     CDS             3325..3474
                     /locus_tag="PLK68_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HRM90272.1"
                     /translation="MAKGVCCHVDTCVYNHDCNCEAKEITVCNCKCHEAKDIQETACE
                     TFKCK"
BASE COUNT         1270 a          405 c          664 g         1216 t
ORIGIN      
        1 cgtaattgtg gaatccgttt acttcgtgat agtgttgttg tatatgaagg aaaattaggt
       61 tcattaaaac gtttccagga tgatgctaaa gaagttgcag cgggatttga atgtggttta
      121 agtattgaaa acttcaatga tattaaagaa ggcgatattg tcgaaggata tatcatggaa
      181 gaagtcgaag ttaagtaggt gaattaaatg attcttaaaa aggataagat gaatggtatt
      241 attcaacgcg agttatcaca aatcattcaa gttgaagtac gtgatcctaa gataggtttt
      301 tgtactatta ctgcagttga tatgactact gatctatcaa ttgctaaagt atatgtgact
      361 ttcttaggaa aagactttga tacgcgcaaa ggaatggaag cattaaatcg ttctaaagga
      421 tttattcgtt ctttattagc taaacgtctt acaattcgta aagtaccaga actgattttt
      481 gttaacgata cctctttaga gtatggtaat aaaatcgaaa aaattattga tgatttaaat
      541 catcatgata aataattcaa aagaaggtca aattgaagtg aagtcaattt cttggacact
      601 aatttagtta tgctattaat aaggattgat cctgtattgt acaggagttg atccttttaa
      661 ttttgttgtt attttttgag tatagtattg ttttatacat tcttctattg caatctttaa
      721 attatataac gatctaaaag tatattcata accataaaaa atttcatttt tccaacggaa
      781 ttcttcattg gtgaattatc taaacaattt cttttttaat attgactgtg gaatttattc
      841 ttttcaaaaa ttttttatac acaatatttt tttgttatct tgtgagttta accataataa
      901 aatgaactcc accatttgaa atacctaaat aatgggacta caataaatca ccttcttttt
      961 atttgagtct tatttgtttc atctagatag aattgtgcta taatactata taggactaaa
     1021 agaaatgaaa tactgtataa caaacgggaa agttatttta aaaaatcaag tagtagatgc
     1081 aaatgtatat gttgaaaata caaagattac tgagatttct aatcgtcaac ctgatgatga
     1141 aacagtaatt gatgcaaaag gtagatatgt gtcaccagga tttattgatg ttcatactca
     1201 tgggcgtggt ggcagtgata caatgtataa cacttttgaa gatcttgata caattacaag
     1261 tactgccgta aaaacaggtg taacaggaat tttacctaca acgatgacga tgtctaaaga
     1321 agatacttat gcggctatta aaaatgttgg agataacatg gataaagttg gaggaagtaa
     1381 aatcctagga gtacacatgg aaggtccatt ttttaataca aaatataaag gagctcaacc
     1441 tgaagagttt atgatcaaac ctacagctga aaattatagc agtttagttg gtgaatatgg
     1501 taaaattgta aaaaaattaa gtttagcacc agaattaaag gattctgata agttaatcga
     1561 gtatttagtc aaagaaggtg tggttgtaag tattgggcac actaatgcaa cttatgacga
     1621 agcagttgtt ggaattaaag ctggagcaac ttcaggtact catacttata atgcaatgac
     1681 accattaaca catcgaaatc caggagtagt aggagctatc atggaacatg atgaagttta
     1741 tgcggaattg attttagatg gaattcatgt cagctatcca gctgcaaaag tgcttttaag
     1801 ggctaaaggt ttagataaag tgattttaat cactgattct attgaagctt ctggtttaga
     1861 agatggtcaa tataaattag gtaatcaagc tgtatttgtt aaagataatt cagctcgttt
     1921 agaagatggg actttagctg gtagtatttt agcaatgaat aatgcagtaa agaatgcata
     1981 tcaacattta ggtttatcaa ttaatgaagc tgttaattta gctagttata atcctgctaa
     2041 aaatttgaat ttaattgatt taggggaaat tgcagttaac aaaacagcgg acattattat
     2101 gtttgatgaa gaaattaatg ttgattttgt aatgattgat ggaaatgtaa aaattggagg
     2161 atagtatgat tacattaaag aatgaagttt tagaggttac tctagctaat aaaggtgcag
     2221 aaattattaa aattgttggg caggatgatc aaattaatta tatgtggcgt cgtgatccaa
     2281 ttcaatgggc gaatagtgct ccaatcctat ttcctattgt aggtgcgcta caaaataatg
     2341 agtgtcgaat tgatggtaaa acttatacta tgactcaaca tggtttttca cgtcatagtg
     2401 aatatgaggc taatcaaatt aatgatcaag aagtagtttt tacacttatt tcaaatgagg
     2461 aaatagctaa acaatatcca tatttattta agttagatgt aacttataaa ttggataaga
     2521 atgtacttag ctgtaattgt aaagttacta ataccgatag tcagactatt tatttccaaa
     2581 ttggtggtca tcctgcattt gcttgtcctt ttatggaaaa tgaatcaagt aatgattact
     2641 atttagaatt tagtgagaat gagactgtta atcaaaaaat tattgatgtt gaacataaag
     2701 gaatgtcaga tataacatta ccgtttttag ataacgaaaa gagatttttt gttcgtcagc
     2761 aattatttga taatgatgca attgttttaa aggacgtgaa atcaaaaaca ttatcattga
     2821 agtcattaaa tcacaataaa tctttagtat tccatatgga aggttttaat cacttaggta
     2881 tttgggcagc taaacatgtg ggtggactaa ttgccattga accttgggta ggtcatagtg
     2941 attatgttgg tttcactggg gaatttaagg ataaagaggg tgtagttagt ctaaatccta
     3001 gtgagatttt tgaatgtact tttaaagttg aaatcaacca ataatgacaa aaaaggaata
     3061 agataaattt tataatcatc ttatgaaagt aaaaattatt gaaaaaacac acgagttaga
     3121 tttagaagat gaaattaatg attttttgga aaagaaaaat cctaaaattt tatcgctgca
     3181 ttatcaagtg gcaatgactt tttcaagtga attagaatat agttttagct gtttgattga
     3241 gtacgaggat aaataatcat atttatcctt ttcttttcgc atacttttag tattttagtt
     3301 aaaactattt atggaggtga ttccatggct aaaggtgtat gttgtcacgt tgatacttgt
     3361 gtttacaatc atgattgtaa ttgtgaggca aaagaaatta cagtttgtaa ttgtaaatgt
     3421 cacgaagcga aagatattca agaaacagct tgtgaaactt ttaaatgtaa atagaaagtt
     3481 taatatgaca tcacaaaagg taagaagtaa cttttgtgat gtcttttgtt ataagtatga
     3541 gctttacaaa aagac
//
LOCUS       DAOMTA010000238         3553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_956_length_3553_cov_2.000572, whole genome shotgun sequence.
ACCESSION   DAOMTA010000238 DAOMTA010000000
VERSION     DAOMTA010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_956_length_3553_cov_2.000572"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1835
                     /locus_tag="PLK68_07405"
     CDS             <1..>1835
                     /locus_tag="PLK68_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRM90273.1"
                     /translation="LFSLRSPKYENHTPLHTIIQTTPKAQIESVVLNIYQDIVDNHMH
                     YHDYAIYYPNQEYLTLLVDTLNNFKIPHNIKKSLIFKELDACLLWIRYCLNHDNNDLL
                     DLLDTKVLSRYNDFDYLDLIKKNYLEKGYIEDPFSTLYNFEKAQSLDDYSSVMINFIN
                     QEMLFSQNQTMLINFFTNLTSPQSFTLADFYSLVNQLKPSLKEDIKPCNDHLYLLNYQ
                     QCYSGILECKKIYLVGVNETVVPKQNKDTGILLDQDYQILQLPDLNHQISVDQNNILK
                     VLNSQTEFTAICFSNATIDGQPLLKSSLYNQLKQMFKISNIDINNDYYHYSLKTNLYL
                     HGGKDIELSSLNTMIKHYIQTNNQPDKLTVPLFSNHLTASKLETYNGCPYKYFNQYGL
                     KLYPFKQPLFQINEIGTIIHYVLEKTQALFTDNITASKAEVDDLETVINQHVEQYLNE
                     HGLTERLNYGTNNYIIKTVKHDLVNTVIVLINQMKASDFYIVGSEVDIYRNYPDFKFS
                     GIVDRVDQYNQYLKIIDYKSSNKDLDLSLAIQGFNIQMLLYLDTLTKQKKLDKGALLY
                     FNTKKRILTSSLKINESELSENFFKLYRMNGYVNSEVVEEVDNNI"
     assembly_gap    1836..1845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1846..2172
                     /locus_tag="PLK68_07410"
     CDS             <1846..2172
                     /locus_tag="PLK68_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90274.1"
                     /translation="DKDSAIIKAKIVKKDDCYKGNILSSFSFERLIDYVSQHIENLYH
                     ELANGNIGINPKGSDDATVFTKVNPCTYCNYRSLCNFDVFYNEYTLVDSNNLEHLLEE
                     DNSDAN"
     gene            2162..3553
                     /locus_tag="PLK68_07415"
                     /pseudo
     CDS             2162..3553
                     /locus_tag="PLK68_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
BASE COUNT         1337 a          529 c          476 g         1201 t
ORIGIN      
        1 acttattttc attacgatca ccaaagtatg aaaatcatac accactacat acaattattc
       61 agactacacc aaaagctcaa attgaaagtg tagtattaaa tatttatcaa gatattgtag
      121 ataatcacat gcactatcac gattatgcta tctattaccc caaccaagaa tatttaacct
      181 tattagttga tactctaaat aattttaaga ttccccataa tattaaaaaa agcttaatat
      241 tcaaagaatt ggatgcttgt ttattatgga ttagatactg tttaaatcat gataataatg
      301 atcttttaga tttattggat actaaagttt taagtcgata taatgacttt gactatcttg
      361 atttgattaa gaaaaattat cttgaaaaag gttatattga agatcccttt agcaccctat
      421 ataactttga aaaagcgcaa tcactagatg attattcatc agtaatgatt aattttatta
      481 atcaagaaat gcttttttca caaaatcaga caatgctaat aaacttcttc actaatttaa
      541 caagtcccca gtcctttaca cttgccgact tttatagttt agtaaaccaa ttaaaaccgt
      601 ctttaaaaga agatatcaaa ccatgtaatg atcatttata tttattaaat tatcaacaat
      661 gttacagcgg tatcttagaa tgcaaaaaaa tctatttggt tggtgtcaat gaaactgttg
      721 ttccaaaaca aaataaagat actggaatat tactagatca agactatcaa attctacaat
      781 tacctgattt aaatcatcaa attagtgttg atcaaaataa tattctaaaa gtcttaaaca
      841 gccaaactga atttacagca atatgtttta gtaatgcaac aattgatggt caaccgctat
      901 taaaatcttc tttgtataac caattaaaac aaatgtttaa aatttcaaat attgatataa
      961 ataatgacta ttatcactat agcctgaaaa ccaatttata tctacatggt ggtaaggata
     1021 ttgaattatc atcactaaat acaatgatta agcactatat acaaactaat aaccaaccag
     1081 ataaattaac tgttccacta ttttcgaacc atcttacagc aagtaaactg gaaacttaca
     1141 atggttgccc atacaaatat tttaatcaat atggattaaa attataccct tttaaacaac
     1201 cgctttttca aataaatgaa attggtacga tcatccatta cgtacttgaa aaaactcaag
     1261 ccctctttac tgacaatatt actgctagta aagctgaagt cgatgattta gaaacagtta
     1321 ttaaccaaca tgtcgagcaa tatttaaatg aacatggttt aactgagcgc ttaaattacg
     1381 gtactaataa ctatattatt aaaacagtca aacatgatct agttaatact gtaatcgttc
     1441 ttatcaacca aatgaaagca tctgattttt atattgttgg tagtgaagtt gatatctatc
     1501 ggaattatcc tgattttaaa tttagcggta tagttgatcg agtagatcaa tataatcaat
     1561 atttaaagat tattgattat aagtcaagta acaaagacct tgacctttct ttggcaattc
     1621 aaggttttaa tattcaaatg ctcttatatt tagatacttt aacaaaacaa aaaaaacttg
     1681 ataaaggtgc tttgttatac tttaatacta aaaaaagaat ccttactagt tctttaaaaa
     1741 tcaatgaatc tgaacttagt gaaaactttt tcaaattata tcgaatgaat ggttatgtta
     1801 atagtgaagt agtcgaagaa gttgataaca atattnnnnn nnnnngataa agattcagca
     1861 atcattaaag caaaaattgt taaaaaggat gactgttaca aaggaaatat tttaagtagt
     1921 ttctcttttg aacgtttaat tgattatgtt agtcaacata tcgaaaattt atatcatgaa
     1981 ttagcaaacg gaaatattgg aattaatcct aaaggtagtg atgatgctac ggtcttcact
     2041 aaagttaatc catgtactta ctgtaactat cgtagtctat gtaatttcga tgtcttctat
     2101 aatgaatata ctttagttga cagtaataat ttagaacact tgcttgagga ggataatagc
     2161 gatgccaatt aattctttaa atgatggtca gtatgaagct gtcgtaaccc gcggatgttc
     2221 cattcttgtt tcagctccag ctggttcagg gaaaactaaa attcttgtta atcgaatcat
     2281 ggctttaatt gaagatgatg gttataatgt cgatcaactg cttgtcttaa cctttactaa
     2341 tgcagccgct cttgaaatga aacaacgtct tcaagttgca ttagatgaac gtcttcaaga
     2401 agatatcaat aagccattgg aacaacattt attaaagcaa aagcaattat tacccaaagc
     2461 ttatattact aatttccatg gtttttgcag tactcttttg aaacaatatg gatatttgat
     2521 caaccttaat agtaaatttg atatttgcac tgatccttca ctcatcaaac accaaattct
     2581 cgataactgt attgaaacat gggcagaaga tcaaacattt attgatttta tcagtactta
     2641 ttttccagaa tactatttta acaactttaa gaacgctatt ttcaaatttg aaaatttaag
     2701 caatactatt tatgactttg ataattatat tgatgcagtc aaaaaagata tttattatca
     2761 cattattaac aacgatgatg atgctataaa caattggcct atcagtaata aaattatcga
     2821 actattacat caacaagcta ctatgggatt gaataaggtt tatgaattag ctagttatgc
     2881 cagttcccat aatttgtctt tttactatca aaatcccttt ggtgataagc ctaagaaagc
     2941 agatttacca agcccatatg attgtcattt aaaatattac ttcgatgtca tcaaggcaat
     3001 agaaagtaaa aacatgacga atatcattac cgctagtaaa gctagtttga tgaaaagtta
     3061 tgatagtaga ggtttattta atgaggataa tgaactttat aaaaaagaat ataatcgtct
     3121 taaaaataat atcatcaaat tttatcgcga taaatttaat gatatcgtat acgatgattt
     3181 tgatgagttt aaattaactt tatcaacctc tttagagcct ttagaaaaat taattactta
     3241 tttaaaacag tttaaacagg cctaccaaga ttataagcta gctcataatc ttttagattt
     3301 caatgatctt gaagctaatg cattagaatt actagaaccc aaatacggaa ttgttacact
     3361 tctataccaa caattaaaat agatcatgat cgatgaatac caagatacta atcagattca
     3421 agaaactcta attaataaaa ttgcagatct tcatgaaccc aagatcaatc gctttatggt
     3481 tggagatatg aaacaatcga tttatcgttt tagagaggct gacccggaaa tttttaatga
     3541 gaaatatctt act
//
LOCUS       DAOMTA010000239         3548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_958_length_3548_cov_2.212139, whole genome shotgun sequence.
ACCESSION   DAOMTA010000239 DAOMTA010000000
VERSION     DAOMTA010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_958_length_3548_cov_2.212139"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLK68_07420"
     CDS             complement(<1..1131)
                     /locus_tag="PLK68_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HRM90275.1"
                     /translation="MNLILKRIGKKSGIIILGFILFMGALCTPFVALNEWLEHPISTT
                     VDIIFGDNSSYSLKENVNVKVLKYKSEVEKWCPKDMNGYLDVSSYVNVILAIMMVESG
                     GEGNDPMQSSECSKNTKYSKKPNSIKDPKYSIECGVKDFADALSQSVTSGLVNNDALF
                     TTVDAYNKGLGIIKRHSKNKKYSFEESCSYCYEHRFNNNKKGYSTASKLRLMFNLQIN
                     KYWDGGNWRWNYGNMFYVYQVLSYLNVYSFNNDGSAGANIINIASSKIGCRYWWGKSG
                     PDYFDCSGLVYWTLNQAGITVPRLTADGFANYGRSISVKELQVGDLIAFDWDWDKKAD
                     HVAIYAGDNFIIHANGNSSIKGNSSKYTVKRQNMTNYYRSHVI"
     gene            complement(1142..3004)
                     /locus_tag="PLK68_07425"
     CDS             complement(1142..3004)
                     /locus_tag="PLK68_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90276.1"
                     /translation="MKLKRTEKVRRTTLAPHGLVEKDNYYIIGDKYVRVLTVSAMPKA
                     ITEGFLAQFISSPDYHIDFSTSLMNIDFASYMQKEINHLEEQINKTNTDTAREELITR
                     YYTMQETIKQMVANKSRTMNVVINIYITADDLQKLNEKTIAVKNRIQTDNHMRLHTLP
                     RLQVGAMQKNSPLFIGNSLNKYVDYHIGVMLPTLTGAALWPFFYDSIDDKYGTSIGVE
                     ANTGGKIIFDQFAYLNDTENAKAQGRTAGNMLVIGKTGMGKTVFMTLLICSHIINHRK
                     VIWLDPENKNRELTRKLEGNYIEVGLNKNVINIFDLQPVSTDADEKESEEVMYDTKIA
                     IKNVVEDMIIMFKTLWPNITEDEINVLDEITEKTYRVVGIDGTESFRYLKPEDYPTFT
                     TFSVVIDQMLEEYENSSLKNKKVIEALLNLQMKMRSLVGTAGIEGHLSRYFNGTTNIN
                     MDSLDTIGLVSFGTKHLMNTPKHIRNALLRIIFKYAWSNCLSTDEQTVFVTDEDHTYI
                     QNEPIAALKAQFQRRARKYNTVTISGTQEVKDYNNDRIQVHGAAIFNNATYRVYFNME
                     LDGIFELGKLVHLTDQEMDTIRFLLPHYCLFENGNRKIPVHILMSDKEKEFFGT"
     gene            complement(2985..>3548)
                     /locus_tag="PLK68_07430"
     CDS             complement(2985..>3548)
                     /locus_tag="PLK68_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90277.1"
                     /translation="ILEWMHIQKADNYGIYLQNQDTEKVVRGLHLSCPNILTIPERQK
                     VQMIVNLSSCFDNYRGELFFKIVKSPLKLHKHLNRYTENLKTEDVFGRKKLTELQIDK
                     MDDAIQERHELAFYVLIQENENVVDNRLSKLDAMLRYAGFTTSLMSITDYEAVISHDF
                     KTEIVNQYEFVSLIIDKEKYENEIEKN"
BASE COUNT         1075 a          680 c          500 g         1293 t
ORIGIN      
        1 tatcacatgt gatctataat aatttgtcat attctgtctt ttgactgtat attttgaaga
       61 atttccttta atggaagaat ttccatttgc atgtataatg aaattatcac ctgcatatat
      121 tgctacatga tcagctttct tatcccaatc ccagtcaaaa gcaatcagat ctccaacctg
      181 taattcttta accgatatac ttcttccgta atttgcaaat ccatctgctg tcagtcttgg
      241 aactgtaata cctgcctgat tcaatgtcca gtaaacaagt cctgaacaat caaaataatc
      301 tgggccactt ttaccccacc agtaacgaca tccaattttt gatgatgcta tgttaatgat
      361 atttgctcca gcacttccat cattattgaa agaataaaca ttcagatagg aaagtacctg
      421 ataaacataa aacatatttc catagttcca tctccagttt ccaccgtccc agtatttatt
      481 gatttgtaaa ttaaacatca atcttagctt tgaagccgtt gaataacctt ttttattgtt
      541 gttaaaacga tgctcataac aatagctgca cgactcttca aaactgtatt ttttattttt
      601 ggaatgtctt ttgataatgc ctaatccctt gttgtaggca tcaactgtag taaacaaagc
      661 atcattatta acaagtcccg aagtaacact ttgtgaaagt gcatccgcaa aatctttgac
      721 tccacattct atagagtatt ttggatcttt gattgaattt ggtttcttag aatatttcgt
      781 attttttgaa cattcagaag attgcatagg atcattacct tctccacctg attcaaccat
      841 catgattgcc aaaataacat ttacatagct agaaacatct aagtagccat tcatatcctt
      901 tggacaccat ttttcaactt cacttttata tttcaacact tttacattga cattttcctt
      961 cagactgtat gaactgttat caccaaagat aatatcaact gtagttgaaa ttggatgttc
     1021 cagccactca tttaaggcaa caaaaggcgt acacaatgca cccataaata aaataaagcc
     1081 aagaataata attcctgact tttttcctat tctttttaaa ataagattca ttttttacct
     1141 gttatgtccc aaagaattct ttttctttat ccgacattaa aatatgcact ggtatctttc
     1201 tgtttccgtt ttcaaacagg caataatgag gaagcagaaa acgaatcgta tccatttcct
     1261 gatctgtcag atgaacaagt ttaccaagtt caaaaatacc atccagttcc atattgaaat
     1321 agactcgata ggttgcatta ttaaaaattg ctgctccatg aacctgaatt ctatcattgt
     1381 tataatcttt aacttcctgt gttccactga tagttactgt attgtatttt ctggcacgtc
     1441 tttgaaactg agcttttaaa gctgcaattg gttcattttg aatataggta tgatcttcat
     1501 ctgtcacaaa tacagtctgc tcatccgttg aaagacaatt agaccatgcg tatttaaaaa
     1561 taattcttaa cagtgcattt ctgatatgtt ttggtgtatt catcagatgc tttgttccaa
     1621 aagaaacaag tccaatcgta tcaagtgaat ccatatttat attagtcgtt ccattgaaat
     1681 agcgtgaaag atgtccttct attccagcag ttcccacaag acttctcatt ttcatttgaa
     1741 gatttaaaag tgcttcaatg acttttttat ttttcaagga actgttttca tattcttcca
     1801 acatctgatc aattactacc gagaatgtcg taaacgttgg ataatcttct ggttttaaat
     1861 atctgaatga ttctgtacca tcaattccaa ctactctata tgttttttct gtaatttcat
     1921 caagaacatt gatttcatct tccgtaatat ttggccacag tgttttgaac ataataatca
     1981 tgtcttcaac aacattttta atagcaatct ttgtatcata catcacttct tccgattctt
     2041 tttcatcagc gtctgttgat actggctgaa gatcaaatat gttaataaca tttttattca
     2101 gccctacttc aatataattt ccttcaagtt ttcttgtcag ttccctgttt ttattttcag
     2161 gatctaacca tataactttg cgatggttaa tgatatgaga acatattaga agtgtcataa
     2221 atactgtttt ccccatgcct gttttaccaa taaccagcat attaccagcc gttcttccct
     2281 gtgcctttgc attttcagta tcatttagat aagcaaactg atcaaagatg attttcccac
     2341 ctgtgttggc ttcaacacca atggaagttc cgtatttatc atcgatactg tcatagaaaa
     2401 atggccataa tgctgcaccc gtaagtgtag gtaacataac accaatgtga tagtcaacat
     2461 atttgtttaa agaattgcca ataaatagtg gactgttttt ttgcatagca cctacctgca
     2521 atcgaggtaa ggtatgaagt ctcatatgat tatctgtctg gattctgttt ttgactgcaa
     2581 ttgttttttc attgagcttt tgaagatcat cagcagtgat ataaatatta ataacaacat
     2641 tcatggttct gcttttgttg gcaaccatct gtttgattgt ttcctgcatc gtataatatc
     2701 tggtaatcag ttcttctctt gctgtatctg tattcgtttt attaatctgt tcctctaaat
     2761 gattgatttc cttttgcata tagcttgcaa aatctatatt cattaaagaa gttgaaaaat
     2821 cgatatgata atcaggtgaa ctgataaact gtgcaagaaa accttcagta attgcttttg
     2881 gcatagcaga taccgttaat actcttacat acttatcgcc aataatataa taattgtcct
     2941 tttcaacaag tccatgaggg gcaagtgttg ttcttctgac cttttcagtt cttttcaatt
     3001 tcattttcat atttctcctt gtcaatgatc agtgaaacaa attcatactg attaacaatt
     3061 tcagttttaa aatcatgtga tataactgcc tcatagtcgg taatggacat taaagatgtt
     3121 gtaaatccag catagcgtaa catggcatct agtttagaaa gtcggttatc tacaacattt
     3181 tcattttcct gaatgagtac ataaaatgcc agttcatgac gttcttgaat agcatcatcc
     3241 atcttatcaa tctgaagttc cgtcagtttc tttcgaccaa aaacatcttc tgttttgaga
     3301 ttttcggtat atctgttcag atgtttgtgc agtttcaaag gactctttac gatcttaaaa
     3361 aacaattctc ctctataatt atcaaaacaa ctggacagat taacaatcat ctgaactttc
     3421 tgcctttcag gaattgtaag tatattagga cacgaaagat gcaatcctct tacaactttt
     3481 tctgtatcct gattctgcag atagattcca taattatccg ctttttgaat atgcatccac
     3541 tcaagaat
//
LOCUS       DAOMTA010000240         3532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_968_length_3532_cov_2.198159, whole genome shotgun sequence.
ACCESSION   DAOMTA010000240 DAOMTA010000000
VERSION     DAOMTA010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_3532_cov_2.198159"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /locus_tag="PLK68_07435"
     CDS             complement(<1..1981)
                     /locus_tag="PLK68_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90278.1"
                     /translation="MRVVEKNLNSSSYHNLFPFFWQHGESNNKIDEYITKMKEQGIND
                     FCIESRPHPAFLEKGWWQSLDFIIEKAKENDMKIWILDDARFPTGYANGKVPEALRKR
                     YLNYRRYDIAGNQKFAQLNLKPIVDMRTFMNNKRHQQDQIFRVVLAKNDISVKDGFNE
                     TDLIDITNQVKDNVLTVSLENNVNYSIFVLYVTMVGDEAVTEEYLDPMKKEATQVLID
                     EVYQKHYDHYYHEFGKTIVGFFSDEPRFGNTKGPNASIGRYNMPLPWNENVLMKLKEI
                     DFDLNNLVLLFQGMSSTANMVRYAYMNIISNLYSENFSQVIGNWCKEKEIDYVGHVIE
                     DNNAHSRLGYGAGHYFRSIAGQTMAGIDIIGGQIVPGMDYHHTAFSTGGSDGEFYHYA
                     LCKLGASAAKLDKNKNGRLMCEAFGAYGWVEGLKMMKWITDHMISHGVNLIVPHAFDP
                     KEFPDWDCPPHFYAHGNNPQYPYFHYFANYANRLCNLMSDGHQICKVGVLYHAFAEWS
                     GDYMLIQKVLKVLQQNQIDCNVISEDYLMEAMIKEDVYQINGYDFEVLVVPYAKRLPD
                     CLLQTIKRLKSKVIFIDAFPEDEEVEGALVLSLQELPRELNDYSEILIDHMEEKLVFY
                     HYQHDDGDIYMFNNESIYSDINSQIMLKTDQSLMIY"
     gene            complement(1992..2474)
                     /locus_tag="PLK68_07440"
     CDS             complement(1992..2474)
                     /locus_tag="PLK68_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM90279.1"
                     /translation="MFRFKKKKQQIEIKAPVVGKSVSIESVPDDTFATKLMGEGVAFK
                     FSGSDICAPVTGELILVADTLHAFGIRDTHGVEILVHIGLETVTLNGAGFIKIKEIGQ
                     KVFEGEPIIKVDQTYMLKSGLNMITMMVVTNSSDYQISLDNLESNVDLDSVMIRCIAN
                     "
     gene            complement(2523..>3532)
                     /locus_tag="PLK68_07445"
     CDS             complement(2523..>3532)
                     /locus_tag="PLK68_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90280.1"
                     /translation="MVAGGIIKGILTICVTLGWMTKTDGNYLTLYAAADALLYFMPII
                     VGFSAGKVFKCNPYVTATIGAALLYPDLVNALAGDTAHHFMGLTITNMSYSQTLLPII
                     LASFIAAKIEYFAKKIIPTMLQLMIVPVIVLMITVPLSWLAIGPVMNTVSSWLSTAVV
                     SIFGFSPILGGILFGAFWQIMVLLGLHSAFIPVLLNNLFTMGYDPINAILGLTVWALA
                     GVSLGYAIKMKDPEKRSLGFGNMASCLCGVTEPTIYSIALPQIKCFVAAWIGGGIAGG
                     ILGALGGKLYSLGGDGLFRIPAMINPNGIDVSFYGFIITALIALVVSAIITYFAADPE
                     K"
BASE COUNT         1211 a          729 c          429 g         1163 t
ORIGIN      
        1 catatatcat taatgattgg tctgttttaa gcattatctg actattaata tcagaataaa
       61 tactttcatt attaaacata tatatatcac catcatcatg ttgataatga taaaaaacta
      121 gtttctcttc catatgatca attaaaattt ctgagtaatc atttagttct ctaggtaatt
      181 cttgaagtga cagcactagt gcaccttcaa cttcctcatc ttcaggaaaa gcatcaataa
      241 aaataacttt actttttaag cgcttgattg tctgtaataa acaatctggt aatcttttag
      301 catacggaac tactaagact tcaaaatcat agccattgat ttgataaaca tcttctttaa
      361 tcatcgcttc catcaaataa tcttcactaa taacattgca atcaatttga ttttgttgta
      421 aaacttttaa tactttttga attaacatat agtccccaga ccattctgca aaagcatgat
      481 ataaaactcc aactttacag atttgatgtc catcactcat taaattacat aatcggttag
      541 catagttagc aaaataatga aaatatggat attgtggatt atttccatgt gcataaaaat
      601 gcggtggaca atcccaatca ggaaattcct tagggtcaaa tgcatgtggc acaattaaat
      661 taactccatg actaatcata tgatcagtta tccacttcat cattttcaat ccttcaaccc
      721 agccataagc tccaaatgcc tcgcacatca aacgaccatt tttattttta tctaattttg
      781 ctgcagaagc acctaattta cacagtgcat aatgataaaa ttctccatca ctgccaccag
      841 tcgaaaaagc tgtgtgatga taatccattc caggaacaat ctgtcctccg ataatatcga
      901 tccccgccat tgtttgacca gcaatgctcc ggaaatagtg acctgcccca taacctaaac
      961 ggctatgagc attattatct tcaattacat gtccaacata gtcaatttct ttttctttac
     1021 accagtttcc tataacctga ctaaaatttt cactatacaa attagaaatg atattcatat
     1081 aagcatagcg taccatatta gctgtactgc tcatcccttg aaataataaa acaaggttat
     1141 ttaaatcaaa atctatctcc tttaacttca ttaaaacatt ttcattccat ggcaaaggca
     1201 tattataacg accaattgaa gcgttaggtc ctttagtatt accaaaacgg ggttcatcag
     1261 aaaaaaaacc aacgatagtt ttaccaaact catgataata atgatcataa tgcttttgat
     1321 atacttcatc aatcaatact tgagtggcct cttttttcat cggatctaag tattcttccg
     1381 taaccgcttc atctcctacc attgttacat ataaaacaaa aatactataa ttaacattat
     1441 tttctaatga tacagtcaac acattatcct taacctgatt tgttatatca atcaaatcag
     1501 tttcattaaa accatctttt acagaaatat cattttttgc taaaacaact ctaaaaattt
     1561 gatcctgctg gtgcctttta ttattcataa aagtacgcat atcaacaatc ggtttcaaat
     1621 taagctgcgc aaacttttga ttccccgcta tatcataacg acgataattt aagtatcttt
     1681 tcctcaatgc ttctggtact ttaccattgg cataacctgt tggaaatcta gcatcgtcta
     1741 agatccatat cttcatatca ttctctttag ccttttcaat aataaaatct aaagattgcc
     1801 accagccttt ttctaaaaat gctggatgag gtcttgattc aatacaaaag tcattaattc
     1861 cctgctcttt cattttagta atatattcgt caattttatt attagattcc ccatgttgcc
     1921 aaaaaaaagg aaataaatta tgatagctgg aactatttaa atttttttct actactctca
     1981 taaattcctc cttaattagc tatacatcta atcataacac tatctaaatc aacattactt
     2041 tcaagattat ctaagctgat ttgatagtca ctagaatttg taactaccat cattgtaatc
     2101 atattaagtc cactttttaa catatacgtc tggtcaactt taataatcgg ctcaccttca
     2161 aatacttttt gcccaatctc ttttatttta ataaaaccag ctccatttag agtaaccgtt
     2221 tctagaccaa tatgtaccaa gatttcaaca ccatgtgtgt cacgaatgcc aaatgcatgt
     2281 aaagtatctg caactagaat caactcacct gtaaccggtg cacaaatatc actaccacta
     2341 aatttaaatg caacgccttc tcccattaat ttagttgcaa aagtatcatc aggaactgat
     2401 tcaattgaga cactttttcc aaccaccgga gcttttattt caatttgttg tttctttttt
     2461 ttaaatctaa acattttttc tcctcataga actaatcttc taggaaacag aagattaatt
     2521 atttattttt caggatcagc agcaaaataa gtaattattg ctgatactac caatgcaatc
     2581 aaagccgtaa taataaaacc ataaaaacta acatcaattc cgttaggatt gatcatcgct
     2641 ggaattctaa ataaaccatc accaccaagt gaatacagtt ttccacctaa agctcctaag
     2701 attccaccgg caattccccc accaatccat gctgctacaa aacatttgat ttgtggtaaa
     2761 gcgatagagt agattgttgg ttctgtcact ccacataaac atgatgccat atttccaaat
     2821 cctaatgagc gtttctcagg atctttcatc ttaattgcat atcctaaaga aacgccagct
     2881 aaagcccaaa ctgtcaatcc taagattgca ttgattggat catagcccat tgtaaataaa
     2941 ttatttaata atactggaat aaaagcactg tgtaatccta ataaaaccat tatctgccaa
     3001 aaagcaccaa acaaaattcc tcctaatatt ggactaaatc caaatatcga tactaccgca
     3061 gttgataacc agctagaaac tgtattcatg actggaccaa tagctagcca gctaagcggt
     3121 actgtgatca ttaaaactat tacaggcaca atcattaatt gcaacattgt cggaataatt
     3181 tttttagcaa aatattctat ttttgcagca ataaacgaag ctaaaataat tggtaataag
     3241 gtttgactat aagacatatt tgtaattgtt aagcccataa aatgatgtgc tgtatcccct
     3301 gctaaagcat ttactaaatc cggatataat agtgctgcac caattgtcgc tgttacataa
     3361 ggattacatt taaatacctt accagccgaa aaaccaacaa tgattggcat aaaatataat
     3421 aatgcatccg ctgccgcata taaggtcaaa taatttccat cagtttttgt catccagcca
     3481 agggttacac aaatcgtcaa tattcctttg ataatccccc ctgcaaccat tg
//
LOCUS       DAOMTA010000241         3526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_971_length_3526_cov_2.298473, whole genome shotgun sequence.
ACCESSION   DAOMTA010000241 DAOMTA010000000
VERSION     DAOMTA010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_971_length_3526_cov_2.298473"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="PLK68_07450"
     CDS             complement(<1..2153)
                     /locus_tag="PLK68_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HRM90281.1"
                     /translation="MKKQLKKIMTIVLTAGMVISLLGTPSFAVEHVGTSDAAKEMYKN
                     TYANLTNRITNRGYAPTSLTGAYEGMFIRDSSIQVMAQNAYGDYQKSRQVLNFLLSYQ
                     QGLGADYAQHIVPDYEDEEFGNSYLSQGETAQTQNLYTSQTFSDHALFGLKGPNNGGG
                     VAFQTDSDKVTSISIFTNSTGNDVLTGSLRTNITDASTELISKEITVTASGWQTFTFD
                     QPITVKKGETYHFFVQSNNGIAMFGKADGKPADNAIMGYNYDVPVLGGFAESAYPAFK
                     LNGNDDEKTDNSFMSNTDHSIALFLITATTNNSAFAFVPNSDSICSFRVYLTGSAPVT
                     FKLTTEPGNEAKTIKTISQTVSSNGWQTVDFGENIPVTPGQKYYVHITTTSGQVIACG
                     SSMFAGSVASMNWENNVWSQTGYVIAAEVFSEVNIAQSPYAQKISLNGDAIQAVSFTA
                     DANVAGGNLIGELRESLNSKAIASGEIAIEKAGVQNYQIDFGKEVKVAADKDYYFVLW
                     VENNDYVQWNYNPALKASAYSLNGDNWDSHDFDFELDILPLYSGDYRVPLMTIGDKNI
                     GIQEIPSLDQYVTAVDVLLGKNNLEEGIAIATLYKGYGAEAIKVDDAAIDISKLSSSG
                     QMVHLEFSLSLEQVDKTQSYYLEIKAPQNSDNTITWYGSKNVDNLATLYNGTAVAGEA
                     GYVAYKSNLHTLSNHVQIDGNYMLIHAWAQFVNGC"
     gene            complement(2483..>3526)
                     /locus_tag="PLK68_07455"
     CDS             complement(2483..>3526)
                     /locus_tag="PLK68_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90282.1"
                     /translation="PLNLAEKYGGWINRKLIEFYLNFASTLFERWKTKVKYWITVNEI
                     NVLGGYWTLGLASNNRKTKSNSNQGETPVEDAGIKLQAIHHLLVASAKANKIARKINP
                     NFMLGTMCALSGIYPMTCKPEDVFGAYEFRRKALMFSDITMRGYYPNYAQSIFDEYKF
                     TLKKELGDDEVLKENTSDYLAFSYYRTTAFDCDASNTTTTGGQQASPNPYLQKTAWGW
                     PIDPKGLRFVLNELYDRYQKPLFIVENGMGNYDIVEEDGAIHDDYRIEYLRSHIVEFK
                     KAVEIDQIPLLGYTTWGCIDIVSAGTGEMAKRYGFVYVDLDDKGNGTFKRKKKDSFYW
                     YQKVIESNGEILG"
BASE COUNT         1124 a          721 c          457 g         1224 t
ORIGIN      
        1 ttacatccat taacaaattg agcccacgca tggatcaaca tataattccc atcaatttga
       61 acatgattgg ataaagtatg aagattggat ttatatgcaa catagcctgc ctctccagct
      121 actgcagtcc cgttatataa tgttgctaaa ttatccacat ttttacttcc ataccaagta
      181 attgtattat cactattttg tggagcttta atttctaaat aataagattg cgttttatcg
      241 acttgttcca acgataaact aaattctaaa tgtaccattt gcccacttga agatagctta
      301 gaaatatcaa tcgcagcgtc atctactttg attgcttctg caccatatcc tttatataaa
      361 gttgcaatag caattccctc ttctaaatta tttttaccta acaatacatc tacagccgtt
      421 acatattgat ctaaagatgg aatttcctga atcccaatat ttttatctcc aattgtcata
      481 agcggtactc gataatcacc cgaatataat ggtaggatat ctaactcaaa atcaaaatca
      541 tgactatccc aattatcacc atttaaacta taggcgcttg ctttaagagc agggttataa
      601 ttccattgta cataatcatt attttcaacc cataaaacaa aataataatc tttatcggca
      661 gcgactttaa cttcttttcc aaaatcaatt tgataatttt gtactccagc tttttcaatt
      721 gctatctccc ctgaagcaat agccttacta tttaaacttt ctcttaattc accaatcaaa
      781 ttaccacctg ccacgttagc atcagcagta aaagaaacgg cttgaattgc atcaccattt
      841 aatgaaattt tttgagcata tggtgattga gcaatattaa cttctgaaaa tacttccgct
      901 gcaataacat aaccagtttg tgaccaaaca ttattttccc agttcataga tgctacagaa
      961 cccgcaaaca ttgaagaacc acaagcaata acttgaccgc tggttgttgt aatatgaaca
     1021 taatattttt gtcctggtgt cacaggaata ttttctccaa aatcaactgt ttgccagcca
     1081 ttacttgata ctgtttgtga aatagttttt attgtttttg cctcatttcc aggttcagtt
     1141 gtcaacttaa aagtaacagg agcacttccg gttaaataaa ctcgaaacga acagatagaa
     1201 tcactatttg gcacaaatgc aaaagcagaa ttattagtag ttgcagtaat taaaaataaa
     1261 gcaatcgaat gatctgtatt tgacataaat gaattatctg ttttttcatc atcatttccg
     1321 tttaatttaa atgccggata tgcgctttct gcaaagccgc ctaaaaccgg aacatcataa
     1381 ttataaccca taatagcatt atccgcaggt ttcccatcag cctttccgaa catagcaata
     1441 ccattattag attgtacaaa gaaatgataa gtttctcctt tttttacagt aattggctgg
     1501 tcaaatgtaa atgtctgcca gccactagca gtaactgtaa tctctttact tatcaactct
     1561 gttgaagcat ctgtaatatt tgttcgcaaa cttccagtta aaacatcatt tccagtagaa
     1621 ttagtgaaaa tagaaattga tgttacttta tcactatctg tttgaaatgc tactccacca
     1681 ccgttatttg gtccttttaa cccaaataac gcatgatcag aaaaagtctg tgaggtatat
     1741 aaattttgtg tctgtgctgt ttctccctgg gatagatagg agttcccaaa ttcttcatct
     1801 tcataatcag gaacgatatg ctgagcataa tctgccccta gtccttgttg atagctcaac
     1861 aaaaaattta atacttgcct tgatttttga taatcaccat aagcattttg tgccattact
     1921 tgaatcgaac tatcacgaat aaacattccc tcataagccc ctgtcaaaga agtcggtgca
     1981 taaccccgat tcgtaattcg attcgttaaa ttagcatatg tattcttata catttcttta
     2041 gccgcatcgc ttgtccctac atgttcaact gcaaaagatg gtgttcctaa taatgaaatt
     2101 accattccag cagttaagac aatagtcatt atttttttta actgtttctt catcttcctc
     2161 acctctctaa tttgtgtgtt gtttactcaa tgtatattgt ctacttttat tattccctcc
     2221 ttttagaaca ggtattttaa tcaaaaagac aagactaaaa agtattacac ttttttggtc
     2281 ttgcctacct aaagtaacaa cccactagta tatctttaat atactattat ttattatcat
     2341 tttaaatctg tctatacaat tttggtacaa tatttcatta aatagtataa ttctggtaca
     2401 ttgtttcact atcccactaa aaaagaagtg attttagaaa ccgttccctt taaataacat
     2461 aattttaaac aaataaaata atctagccta gaatttcgcc atttgattca atcacttttt
     2521 gataccagta aaatgaatct tttttctttc ttttaaaagt accattacct ttatcgtcca
     2581 aatccacata aacaaaacca tatctttttg ccatttctcc tgtacccgcc gatacaatat
     2641 caatacaccc ccaggtcgtg tatccgagta aaggaatttg atctatctct actgcctttt
     2701 tgaattcaac aatatgacta cgcaaatact caatacgata atcatcatga atcgctccat
     2761 cttcttcaac aatatcataa ttacccattc cattttcgac aatgaatagt ggtttttgat
     2821 aacggtcata taattcattt aaaacaaacc taagaccttt gggatcaata ggccatcccc
     2881 acgcagtttt ttgtaaataa ggattgggtg atgcttgctg ccctcctgtc gttgttgtat
     2941 tacttgcatc acaatcaaat gctgttgtac gataatacga aaaagctaaa tagtcagatg
     3001 tattttcttt tagcacctca tcatcaccca gctctttttt taaagtgaac ttatattcat
     3061 caaaaatact ttgagcataa ttaggataat atcctctcat cgtgatgtct gaaaacatca
     3121 acgcttttct tctaaattca tatgctccaa atacatcttc tggtttgcaa gtcataggat
     3181 atattcctga taaagcacac attgtcccga gcataaaatt aggatttatt tttctagcta
     3241 ttttatttgc cttagcactt gccactaata aatgatgaat tgcctgcagc ttgattccag
     3301 catcctcaac aggtgtttcc ccctgattag aattactttt agtcttccta ttatttgatg
     3361 caagacctaa tgtccaatat ccacctaaaa cattaatctc atttactgta atccaatatt
     3421 tgacttttgt tttccagcgt tcaaacagtg tagaagcaaa attcaaataa aattctatta
     3481 atttacgatt tatccatcca ccatattttt cagccaaatt caatgg
//
LOCUS       DAOMTA010000242         3525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_973_length_3525_cov_2.280403, whole genome shotgun sequence.
ACCESSION   DAOMTA010000242 DAOMTA010000000
VERSION     DAOMTA010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_3525_cov_2.280403"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLK68_07460"
     CDS             <1..241
                     /locus_tag="PLK68_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRM90283.1"
                     /translation="HTKTVLLSTASPYKFPESVYQAIYGEELDAYTAIDKLSEKTGVP
                     VPQALAGIKEREVLHKEAIDKTEIISFIKSEIEAM"
     gene            251..1144
                     /gene="thrB"
                     /locus_tag="PLK68_07465"
     CDS             251..1144
                     /gene="thrB"
                     /locus_tag="PLK68_07465"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRM90284.1"
                     /translation="MKVKVKVPATSANLGPGFDVAGLALTLYNTFTFELADQGLNITG
                     CPEQFCNEENMTYQAFKQAAEICGLEYQGVNIECSGDVPYTRGLGSSSTCIVAGIVGA
                     FAFKDKVEDRQEILELATAIEGHPDNVAPAIFGGLTVSVMEEDNVLTLNIPVKHDYRF
                     VTLIPPFTLSTEQSRSVLPQVLPRADAIKNVSHLALMVASLINGYDEGLKLGFKDRLH
                     QPYRGDLIKGFNEIMGVLEQDEKVLGAYLSGAGPTIMAVIRGEDKMGVVRIKEELGAL
                     IKDWQVVKLELDNRGYTADYE"
     gene            complement(1174..1392)
                     /locus_tag="PLK68_07470"
     CDS             complement(1174..1392)
                     /locus_tag="PLK68_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90285.1"
                     /translation="MQYKRLKELRTKYGYTQEKVAKYLHVDQKTYSRYELGQHEMTPD
                     TLGKLATLYDTSVDYLIERTDNIKSFTK"
     gene            complement(1484..1714)
                     /locus_tag="PLK68_07475"
     CDS             complement(1484..1714)
                     /locus_tag="PLK68_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90286.1"
                     /translation="MYYKRIRDLREDKDLTQKQLAEYLNVSQKSYSRYERGERTIDPE
                     ILSKLATFHETTVDYLIERTDDKTDYTKKKRR"
     gene            1852..2538
                     /locus_tag="PLK68_07480"
     CDS             1852..2538
                     /locus_tag="PLK68_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRM90287.1"
                     /translation="MEQFARITRMVGSSWIECLQNKKVAVFGLGGVGSYVVEALVRNG
                     IGELVLVDHDIIDITNLNRQLYALHSTIGMNKVDVARARCLDINPNLKITTYQQFYLP
                     DQGMETIFEDCDFVVDAIDTVKAKLALIENCQHLGVRVISSMGTGNKLDPSRFEITDI
                     YKTSVCPLARVMRRELKNRGIKKCPILYSTESPQEVDGSTPGSVSFVPSVAGLMIAGY
                     VIKELVKEEE"
     gene            2538..2780
                     /locus_tag="PLK68_07485"
     CDS             2538..2780
                     /locus_tag="PLK68_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90288.1"
                     /translation="MEGITEINKDDYIDNCLKIVKEMITEEDFSDEIWLALTGEIMDT
                     CLFIGGDFEEANIRNITNQYINNGGIKRFKKAHEVL"
     gene            2780..3520
                     /locus_tag="PLK68_07490"
     CDS             2780..3520
                     /locus_tag="PLK68_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM90289.1"
                     /translation="MQHYCEIPTPKGTMRGFFHKPDLDRHPVCIIFHGFTGQKTGTKF
                     CYVQLARMLEAKGIATFRFDFLGSGESDLNFKDMTFKDELACARVILEEALKMENCTE
                     IYVLGHSMGGAVASELAKLYPQVISKLVLWAPAFNLPAALDYLTGKVEPSSNGLYDHG
                     GYEISQTFVDDILARDFYADLDTYKNELLVIHGTNDTTVPFDISKIYVPKFNQQLKFV
                     PIEGANHNYDTVEHIKEVLKLSLDFLTK"
BASE COUNT         1151 a          461 c          743 g         1170 t
ORIGIN      
        1 ccatactaaa acagtattat tatcaactgc atctccttat aagttccctg aatcagttta
       61 tcaagctatt tatggggaag aacttgatgc ttatacagca attgataaat taagtgaaaa
      121 gactggtgtt cctgtgcctc aagcattagc aggaattaaa gagcgtgaag tgttacataa
      181 agaagcgatt gataaaactg agattattag ttttatcaaa tcagaaatag aagcaatgta
      241 ggaggttagc atgaaagtaa aagtaaaagt accagcgaca agtgctaact taggacccgg
      301 atttgacgtt gcagggttgg cactgacttt atataatacc tttacctttg aactggctga
      361 tcaaggttta aatattactg gttgtccaga acagttttgt aatgaagaaa atatgaccta
      421 ccaggctttt aaacaagctg ctgaaatttg tggcttagaa tatcaaggcg taaatattga
      481 atgtagtggg gatgttcctt atacccgtgg attgggtagt agttctactt gtattgtagc
      541 aggaatcgtt ggggcttttg cttttaaaga taaagttgaa gatcgacaag aaattctaga
      601 attagcaact gcaattgaag gtcatcctga taatgtggca ccggcaattt ttggaggctt
      661 gacagtatca gtaatggaag aagataatgt attaacatta aatatccctg ttaaacatga
      721 ctatcggttt gttacattga ttccgccatt tacattatca actgaacaat ccagatcagt
      781 tttaccacag gttttgccaa gagctgacgc gattaaaaac gtatctcatt tagcgttgat
      841 ggttgcttca ttgattaatg gatatgatga ggggctaaaa ttaggcttta aagaccgttt
      901 gcatcaacca tatcgtggtg atttaattaa aggctttaat gaaatcatgg gagttttaga
      961 acaagatgaa aaagtacttg gggcttattt gtcgggggct ggtccaacaa taatggctgt
     1021 tattcgtggt gaggataaga tgggtgtagt tcggattaaa gaagagctag gtgcattaat
     1081 taaagattgg caagttgtga aattggaatt agataatcgt ggttacacgg ctgattatga
     1141 ataataaaaa acgtttggaa catcaaacgt tttttattta gtaaaacttt ttatattatc
     1201 agttctttca attagataat caacggaggt atcatagaga gttgcaagtt ttccaagcgt
     1261 atcaggcgtc atttcgtgtt gacctagttc atagcgggag tatgtttttt gatcgacatg
     1321 taaatatttg gctacttttt cttgagtgta gccatattta gttcgaagct cttttaatcg
     1381 tttgtattgc atcatatcac cgcctttttc aattatagat tagtctaatt aggactagaa
     1441 gtttttagac caaaacggtc taaaataata aaacgctagt taactagcgc cgttttttct
     1501 tagtgtaatc agttttatca tcagtacgtt cgatcaagta atcaacagtg gtttcatgaa
     1561 atgttgcgag tttggataaa atctctggat cgatggttct ttctcctcgt tcgtaacgag
     1621 aataagattt ttgagagaca tttaaatatt ctgctaattg tttttgagta aggtctttat
     1681 cttctcttaa atcacgaatt cttttgtaat acataaaacc accacctaaa tttattatag
     1741 agtggacctt attggtctat gcataaatgt cctaaattgg actaagaaaa aattatttgc
     1801 ttattcttta taaaagagat agattagttt ataataagca cgaggtggaa tatggaacag
     1861 tttgcaagaa ttacaaggat ggtaggatct tcatggatcg agtgtcttca aaataaaaaa
     1921 gttgcagttt ttggtttagg gggagttggg agctatgtag ttgaggctct tgttagaaac
     1981 gggatcggcg aattagtttt ggttgatcat gatattattg atataactaa tttgaatcgt
     2041 cagttatatg cgttgcattc aacgattggg atgaataaag tagatgttgc tagagcacga
     2101 tgtttagata ttaatcctaa tctaaagatc actacctacc aacagtttta tttaccagat
     2161 caaggaatgg aaactatttt tgaagattgt gattttgtgg ttgatgcaat tgatactgtt
     2221 aaggctaaat tggcactaat tgaaaattgt cagcaccttg gagttagagt catttcatca
     2281 atgggaacag gtaataaatt agatccaagc cgttttgaaa ttacagatat ttataaaact
     2341 agtgtgtgtc cattagcacg agtaatgcgt cgtgaactaa agaatcgggg cataaaaaaa
     2401 tgtcctattt tatattcgac agaatctccg caagaagttg acggcagtac accaggtagt
     2461 gtgagttttg ttcctagtgt agctggtctg atgattgcgg ggtatgtaat aaaagaatta
     2521 gtgaaggagg aagaataatg gaaggaatta cagaaattaa taaggatgat tacattgata
     2581 attgtttaaa aattgtcaaa gagatgatta ctgaagaaga ttttagtgat gaaatctggc
     2641 ttgctttaac tggtgaaatt atggatacat gtttatttat tggtggtgat tttgaagaag
     2701 caaatattcg taatattaca aaccaataca ttaataatgg tggaattaaa cgctttaaaa
     2761 aagcgcacga ggtgttgtaa tgcaacatta ttgtgaaatt cctactccaa aaggaacgat
     2821 gagaggattt tttcataagc cggatttaga tagacatcca gtatgtataa tttttcacgg
     2881 ctttaccggt caaaagacgg ggacgaagtt ttgttatgtt caattagcaa ggatgctgga
     2941 agcaaaagga atagctacat ttagatttga ttttttaggt agtggggaaa gtgatcttaa
     3001 ttttaaagat atgactttta aagatgagtt ggcttgtgca cgtgttattc tagaggaagc
     3061 attaaaaatg gaaaactgta ctgaaattta tgttttagga cattctatgg gcggtgcagt
     3121 tgctagtgaa ttagcgaagt tatatccaca agtaattagc aaattagtat tatgggctcc
     3181 tgcttttaat ttaccagcag ctttagatta tcttactggt aaggttgagc caagttctaa
     3241 tggattatac gatcatggcg gttatgaaat ctctcagaca tttgtagatg atattttggc
     3301 tagagatttt tatgctgatc tagatacata taaaaatgaa ttattagtaa ttcatggtac
     3361 aaatgataca actgtaccat ttgatattag taaaatatat gttcccaagt ttaatcaaca
     3421 attaaaattt gtaccaattg aaggggcaaa ccataattat gatactgttg aacatattaa
     3481 agaagtttta aaattatcgt tggatttttt aacaaagtaa gtaga
//
LOCUS       DAOMTA010000243         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_977_length_3522_cov_2.063455, whole genome shotgun sequence.
ACCESSION   DAOMTA010000243 DAOMTA010000000
VERSION     DAOMTA010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_977_length_3522_cov_2.063455"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLK68_07495"
     CDS             <1..550
                     /locus_tag="PLK68_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRM90290.1"
                     /translation="GEAYDKVGRVLNLPYPGGPIIDKMAAKGNHTYNLPVPLDDESYN
                     FSFSGLKSAVINLNHKAMQRNEEINQEDLAASFQDVVLSVLVNKTIRATKEYGIKQVL
                     MAGGVSANRGLRNAMGEAVGRLDGVELLLPPMSCCTDNAMMIALAAKQMYDLKLFSDL
                     SLGIKPNLDLENESVSGGNQNG"
     gene            543..1172
                     /locus_tag="PLK68_07500"
     CDS             543..1172
                     /locus_tag="PLK68_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRM90291.1"
                     /translation="MDKKISNATMSRYPVYLKALRKMQHEGKENCLSSELSSLTGIQD
                     TTIRRDFTYLSKTDNFGQRGKGYDVKHLIDGLSEVLGLGLDESIILIGIGNLGSAILK
                     YNRWQYTVGKIVCGYDQDKNKEGERFGVKLYNIDDLEKTFPKDCKIAILAISENVQPT
                     VDRLMDLGIKGIVDFTHTHFTVREGVEVQQVDVVVAIQELVIKMDSNRQ"
     gene            1228..2613
                     /gene="asnS"
                     /locus_tag="PLK68_07505"
     CDS             1228..2613
                     /gene="asnS"
                     /locus_tag="PLK68_07505"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRM90292.1"
                     /translation="MFEFITVRELYEMFLSNQMMDLEGYEYVELEGWVRTNRNNGKLG
                     FIALNDGTYFKNLQVVYTEAEISNYEEIDKLSTGSSIRVVGKLKLTPEGKQPFEVEAT
                     EIEIEGKCDEDFPLQKKRHSFEYMREIPHLRPRANTFYAIFRLRSVLSMAIHEFFQSQ
                     GFVYVHTPIITGNDGEGAGEMFRATTIDGTNFEDDFFEKEAFLTVTGQLHVEAFAMAF
                     RDVYTFGPAFRAENSNTSRHASEFWMIEPEIAFADLEDDMDLIEDMVKYCIDYVLENA
                     PAEMEFFASMIDKDCINRITKVKNSDFKRMTYTEAIEILEKADVKFKNKVSWGMDLNS
                     EHERYICEQVVKGPVFLTDYPKEIKAFYMRLNDDNKTVAACDLLVPGIGELVGGSQRE
                     ERYDVLERIMDEKGMSKDGLQWYMDLRRYGGCKHAGFGLGFDRFLMYLTGMQNIRDVE
                     PFPRTPRNLKF"
     gene            2687..>3522
                     /locus_tag="PLK68_07510"
     CDS             2687..>3522
                     /locus_tag="PLK68_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM90293.1"
                     /translation="MNEYDLINAYLNLIFDDSITYFSRILDEYRGLCQLRKWMRKRGI
                     NLKAIENIGYDMFLAFSKAKNAEQIMEKMDPTHLEELKEYINTERNKYQIVVLSSFDY
                     SEPINAYLTSNQIVDDLDSDNIRVLNCHNLLKKEDLSFYGPFKHFVTALYQIDRWPGF
                     LIFKGDECAFVPIYTTQDIEEVIFKIEHDTIFSIRYLSPYDSFFVQLSDLHLGSKKKN
                     IGRLALEDSLDQTVKQLRSYYPLKFLITGDLMNSPNRKNMYEASGFMKGLKNRYNADV
                     SF"
BASE COUNT         1232 a          417 c          711 g         1162 t
ORIGIN      
        1 tggagaagca tatgataaag tgggacgagt tttaaattta ccatatcccg ggggacctat
       61 aatcgataaa atggctgcta aaggtaatca tacttataat ttaccagtgc ctttggatga
      121 tgaaagctat aattttagtt ttagtggatt aaaatctgct gtaattaatt taaatcataa
      181 agcgatgcag cgaaatgaag aaatcaatca agaagattta gctgcctctt ttcaagatgt
      241 ggttttatcg gttttagtca ataaaacaat acgtgctact aaagaatatg gtattaaaca
      301 agtattaatg gcaggaggcg tgagtgctaa tcgtggattg cgaaatgcga tgggtgaagc
      361 ggttggccga ttagatggag tagaattatt attaccgcca atgagttgtt gtactgataa
      421 cgcaatgatg attgctttag cagcaaaaca gatgtacgat ttgaaattat ttagtgattt
      481 gtcattagga attaagccaa atttagattt agaaaatgaa tcggttagcg ggggaaatca
      541 aaatggataa aaaaatatca aacgctacaa tgtctcgtta tcctgtttat cttaaagctt
      601 taagaaaaat gcagcatgaa ggtaaagaaa attgtttatc aagcgaatta tctagtttaa
      661 caggtattca agatacgacg attcgtagag attttactta tttatcaaag acagataatt
      721 ttggccaacg tggtaaaggt tatgatgtaa aacatttaat tgacggatta agtgaggtat
      781 taggattagg actagacgaa tcaattattt tgattggtat cggaaatctt ggtagtgcaa
      841 ttttaaaata taaccgttgg caatatacgg tgggaaaaat tgtctgcggc tatgatcaag
      901 ataaaaataa agagggagag cgttttggtg ttaaactcta taatattgat gatttagaaa
      961 aaacttttcc taaagactgt aaaattgcaa tcttggctat ttctgaaaac gttcaaccaa
     1021 cagttgatcg tttaatggat ttaggaatta aaggtatcgt tgattttaca catacacatt
     1081 ttacagttcg tgaaggagtc gaggtacaac aggtagatgt tgttgtagca attcaagaat
     1141 tagttattaa gatggattca aatcgtcaat aatacttgaa taatggataa aataaagtat
     1201 ataataggaa aaactaggag gattaatatg tttgaattta taacggttag agaactatat
     1261 gaaatgtttt taagtaacca aatgatggat ttagaaggtt acgaatatgt tgaacttgag
     1321 ggatgggttc gaactaatcg taacaatggg aaattgggat ttattgctct aaatgatggt
     1381 acatacttca aaaatttaca agttgtatat acagaagcag aaatttctaa ctatgaagaa
     1441 atcgataaat tatctactgg tagttcaatt cgagtagttg gaaaattaaa gttgacacct
     1501 gaaggaaaac aaccatttga agttgaagca actgagattg aaatcgaagg taaatgtgat
     1561 gaagattttc cattacaaaa gaaacgtcat tcattcgaat acatgcgtga aataccgcat
     1621 ttaagacctc gtgcgaacac tttctatgca atttttagat tgcgcagtgt tttatcaatg
     1681 gctattcatg aatttttcca atcacaaggg tttgtttatg ttcatacacc aattatcact
     1741 ggaaacgatg gtgaaggggc cggagaaatg tttagagcaa caactattga tggaactaat
     1801 tttgaagatg atttctttga aaaagaggct ttcttaacag ttactggtca attacatgtt
     1861 gaagcttttg caatggcttt tagagatgtt tatacatttg gtcctgcatt tagagcagaa
     1921 aactctaata cttctcgcca tgctagtgaa ttttggatga ttgaacctga aattgctttt
     1981 gctgatttag aggatgatat ggatttaatt gaagatatgg ttaaatactg tattgactat
     2041 gttttagaaa atgctcctgc ggaaatggaa ttctttgctt caatgattga taaagattgc
     2101 atcaatcgaa ttactaaagt taaaaacagt gatttcaaac ggatgacata cactgaagca
     2161 attgaaattt tagaaaaagc agatgttaaa tttaaaaata aagtttcatg gggaatggat
     2221 ttaaatagtg aacatgagcg ctatatttgt gaacaggttg ttaaaggacc agttttcttg
     2281 acagactatc caaaagaaat caaggctttc tatatgcgtt taaatgatga taataaaaca
     2341 gtggctgctt gtgatttatt ggttccagga attggagaat tagttggagg aagtcaacgt
     2401 gaagaaagat atgatgttct tgaaagaatc atggatgaaa aaggaatgtc taaagatggt
     2461 ttacaatggt atatggactt acgtcgctat ggtggctgta aacatgcggg attcggttta
     2521 ggatttgatc gtttcttaat gtatttaact ggaatgcaaa atattcgtga tgttgaacct
     2581 ttcccaagaa caccaagaaa tttaaaattt taacaaaaag gcctatattt tatataggct
     2641 tttgctttga atgggttata ataatagata aggaggcgat caaataatga atgagtatga
     2701 tttaataaat gcttatctaa atttaatttt tgatgatagc ataacttatt ttagtcgcat
     2761 tcttgatgaa tatcgtggtt tatgccagtt gagaaaatgg atgcgtaaac gtggtattaa
     2821 cttaaaagcc attgaaaata ttggttatga tatgttttta gcttttagta aagctaaaaa
     2881 tgctgaacaa atcatggaaa aaatggatcc gactcattta gaagaattaa aagagtatat
     2941 taatacagaa cgtaataaat atcagatcgt agtattatca agttttgatt atagtgagcc
     3001 aattaatgcg tatttaacct caaatcagat tgtggatgat ttagacagtg ataatattag
     3061 ggttttaaat tgtcataatc tattaaaaaa agaagattta tcattttatg gaccttttaa
     3121 acattttgtt acggccttat atcaaattga tcgctggcct ggatttttaa tttttaaagg
     3181 tgatgagtgt gcatttgttc ctatttatac tactcaggac attgaagaag tcatttttaa
     3241 aattgaacat gatacaattt tttcaattcg atatttaagt ccttatgata gcttttttgt
     3301 gcagctaagt gatttacacc ttggtagcaa aaagaagaat atcggtcgtt tggcacttga
     3361 agatagtctt gatcaaactg ttaaacagct acgttcttat tatcctttaa agtttttgat
     3421 tacgggggat ttaatgaatt caccaaatcg aaaaaatatg tatgaggcat cagggtttat
     3481 gaaagggtta aaaaatcgtt ataatgcaga tgtttccttt at
//
LOCUS       DAOMTA010000244         3501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_985_length_3501_cov_2.537725, whole genome shotgun sequence.
ACCESSION   DAOMTA010000244 DAOMTA010000000
VERSION     DAOMTA010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_3501_cov_2.537725"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLK68_07515"
     CDS             <1..1184
                     /locus_tag="PLK68_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90294.1"
                     /translation="VMIMNNIMTIVKKEFKDILRDRKTLLMTFVIPIIVMPLLFTFIF
                     SSIGDMASPSEDNKYKIVLETNNPEITALFKEAGIYELVNSKDPINEAYDGKIVAYII
                     ANDINESLTQGSTPEVDLYYDTTSQRAMTAISTVQTMFSTYQNSYLASYLEANHLSSK
                     VLQPFTYNEHAKDEEADSMSLMMLGMLIPMMIIGYSGSGIVPIATDLGAGEKERGTLE
                     PLLSTSISRSSILIGKLIVTATFGSITSILSAVGLLLAFKFGMSDTMAITIDLSLQSM
                     LLIVLLAVLYVIFISAVMLLVSTYARSLKEANTYLTPVTLIPVLLSVITMYMEPSSVS
                     MAMMNIPILNVVIVIKEIIFNQLNYVHLYMTIGWSIVYVIVSMIAAKMMYEKEEVIFR
                     A"
     gene            complement(1219..2385)
                     /locus_tag="PLK68_07520"
     CDS             complement(1219..2385)
                     /locus_tag="PLK68_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRM90295.1"
                     /translation="MFKFISKKIIKNYNDYNNPKVREQYGMLCSIVSIACNIVLVIFK
                     LTMGAITHSIAIQADGLNNLSDVGSNLASLFGFKLANRHPDSEHPYGHGRIEYVAGLI
                     IAFLILLVGFQALKDSIFKIIAPEKVTFTIVAVVILIVSILIKLWMAMFNRTVSKNIN
                     SATLMAASQDSLNDVMATSATLISLILSLYTDLPVDGIMGAVVSIIVLKAGIDIFKDT
                     VDPLLGMAPDKELINEIEEYILSYPEALGIHDLMMHDYGPGRKFLTLHVEVDCNDNIM
                     AVHDAMDLIERSMLEKYHILTTIHMDPVDTNDALTNELKQVVLGVVKGINKEYSIHDF
                     RIVTGPTHTNLIFDVMIPSNDEIKHKVLKEEINTKLRAINPNYYTVMQIEHSFI"
     gene            complement(2385..2732)
                     /locus_tag="PLK68_07525"
     CDS             complement(2385..2732)
                     /locus_tag="PLK68_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90296.1"
                     /translation="MELYNKETLKALKESVDDSTYYLPKALFQGSKFGNIRLETKLAY
                     AALLDTLLRKPVFNQENIALLKIDNPVIAQTLAAMANKEVNQAKVAKYLDELIEANLI
                     EINKQDIYIYHID"
     gene            complement(2818..>3501)
                     /locus_tag="PLK68_07530"
     CDS             complement(2818..>3501)
                     /locus_tag="PLK68_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRM90297.1"
                     /translation="ICFTDHRDFDYPIDVFDLDVEGYYQTIMALKKEFADKIIIKWGI
                     EIGLDMNHQLEINNLINQYPFDFVIGSIHVIDHTEFYYGDFFKNLNKEQAHQAFFEET
                     LRCVKNFDCFNVLGHLDYIMRYGPYEDKKVEHKKYQNIIDEIFKNLIIKNKGIEVNTS
                     GYAVNQTCGFPNFEQVQRYYDLGGRIITIGTDSHTSDRVGQYVNDVKENLIKIGFEDV
                     STFTGRLKD"
BASE COUNT         1216 a          583 c          473 g         1229 t
ORIGIN      
        1 aggtaatgat catgaataat attatgacaa ttgtaaaaaa agaatttaaa gatattttac
       61 gtgatcgtaa aacattacta atgactttcg ttatcccaat catagtcatg cccctattgt
      121 ttacctttat cttttcatca attggcgata tggcatcacc aagcgaagat aataaatata
      181 aaattgtttt agaaacaaat aatccggaaa tcactgctct ttttaaagaa gcgggtattt
      241 acgaacttgt taatagcaaa gaccctatta acgaagctta tgatgggaaa atcgtagctt
      301 acatcatcgc taatgatatt aatgaatcat taacacaagg aagtacacct gaagttgacc
      361 tatattatga tacaacctcg caacgtgcaa tgactgctat aagtactgtt caaacaatgt
      421 tcagtactta tcaaaatagt tatttagcat catatttaga agcaaatcat ttatcaagta
      481 aagttttaca accatttact tataatgaac atgctaaaga cgaagaagct gatagtatgt
      541 ccttaatgat gctaggaatg ttaattccta tgatgatcat tggttattct ggttcaggaa
      601 tcgtacctat tgcaaccgat ttaggtgctg gtgaaaaaga acggggaact ttagaaccat
      661 tactttcaac aagtatttct agaagttcta ttttaattgg taaactgatt gtcactgcaa
      721 cttttggtag cattacttca attttatcag cagtcggttt actgcttgct tttaaatttg
      781 gaatgagtga tacgatggcc atcactatcg atttatcact tcaaagcatg ctgttaatcg
      841 tattattagc agtattatat gtaatcttta tttctgcagt gatgttatta gtttcaacat
      901 atgctcgttc tttaaaagaa gctaatacgt atttaactcc tgtaaccctt attccggtgt
      961 tactttcagt aattacaatg tatatggagc caagttcagt ttcaatggcg atgatgaata
     1021 tcccaattct caatgttgta atcgttatta aagaaattat attcaatcaa ttaaattatg
     1081 ttcatctata tatgacaatt ggctggtcaa tcgtttatgt aattgtttca atgattgcag
     1141 ctaaaatgat gtatgaaaaa gaagaggtaa tctttagagc ataaagagcc gctgcagata
     1201 aactgcagtg gctctttttt aaataaaact atgttcaatt tgcataaccg tatagtaatt
     1261 agggttaatt gcacgtagtt tcgtattgat ttcttctttt aaaactttat gtttaatttc
     1321 atcatttgaa ggaatcataa cgtcaaaaat taaatttgta tgtgttggtc cagtaacaat
     1381 tctaaaatcg tgaatactgt attctttgtt aattcctttg acaactccta agactacttg
     1441 ttttaattca tttgttagag catcatttgt gtctactgga tccatatgaa tcgttgtcag
     1501 gatatgatat ttctccaaca ttgaacgctc aatcaagtcc atcgcatcat gaactgccat
     1561 aatattatca ttacaatcaa cttcaacatg caaagttaaa aattttctac ccggtccata
     1621 atcgtgcatc attaaatcat ggattcctaa agcttctgga taagaaagaa tatattcttc
     1681 gatttcatta atcaattcct tatcaggtgc catccccaat aaaggatcaa cagtatcttt
     1741 aaagatatca attcctgctt ttaaaacaat aattgataca acagctccca ttatcccatc
     1801 tactggtaaa tcagtataaa gggataaaat taatgaaatt aaagtcgctg atgtcgccat
     1861 cacatcgttt aaactatctt gactggcagc cattaaggta gcagaattaa tatttttaga
     1921 aaccgttcga ttaaacattg ccatccataa tttaattaaa attgaaacaa ttaaaataac
     1981 tacagctacg attgtaaaag ttactttttc aggagcaatg atcttaaaaa tagaatcttt
     2041 taaagcttga aaaccaacta ataaaattaa aaaagcgatt attagaccag ctacatattc
     2101 aattctacca tgaccataag gatgctctga atcaggatga cgattagcta atttaaaacc
     2161 aaataatgaa gctaaattac taccaacatc tgaaaggtta ttcaacccat cagcctgaat
     2221 ggcaatacta tgagtaatag cccccattgt aagtttaaaa attactaaaa caatattgca
     2281 ggcaatcgaa acgattgaac aaagcatgcc atactgttct cttactttag ggttattata
     2341 atcattataa ttcttaatga tttttttaga tataaattta aacattaatc aatatgataa
     2401 atataaatgt cttgtttatt aatctcgatt aaattagctt ctattagttc atctaggtat
     2461 ttagctactt ttgcctgatt tacctcttta tttgccatcg ctgccaaagt ttgagcaatt
     2521 actggattat ctatttttaa caaagcaata ttttcttgat taaaaacagg ctttcttaat
     2581 aatgtatcta gtaaagcagc atatgctagt tttgtttcta aacgaatatt tccaaattta
     2641 ctgccttgaa ataaagcttt tggcagataa taagtactat catcgacact ttctttcaat
     2701 gcttttaatg tttccttgtt gtataattcc atatttttac ctccaaatat attatccatt
     2761 ataccataaa tgattaatga ttatgataat aaattaaaaa gattagattt ttgctatcta
     2821 atcctttaaa cgccctgtaa atgtactaac atcctcaaag ccgattttaa ttaaattttc
     2881 tttaacgtca ttgacatact gaccaactcg atcacttgta tgactatctg taccaatagt
     2941 aataatacga cctcctaaat cataataacg ttgaacttgt tcaaaatttg gaaagccgca
     3001 agtctgatta actgcatatc cagaagtatt tacctcaatc cccttatttt taataatcaa
     3061 atttttaaat atttcatcaa ttatattctg atatttctta tgttcaactt ttttatcttc
     3121 ataaggcccg tatcgcataa tgtaatctaa atgtcctaaa acattaaaac agtcaaaatt
     3181 tttaacgcac cttagcgttt cttcaaagaa agcctgatga gcttgctctt tatttagatt
     3241 tttaaaaaaa tccccataat aaaattcagt atgatcaatt acatgaattg agccaattac
     3301 aaaatcaaaa ggatactggt taatcaaatt attaatctct aattgatgat tcatatctag
     3361 acctatttca atgccccatt ttattataat tttatctgca aattcttttt ttaaagccat
     3421 aattgtttga taataacctt cgacatctag atcaaaaaca tcaattgggt aatcgaaatc
     3481 acgatgatca gtaaaacaaa t
//
LOCUS       DAOMTA010000245         3500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_987_length_3500_cov_1.930334, whole genome shotgun sequence.
ACCESSION   DAOMTA010000245 DAOMTA010000000
VERSION     DAOMTA010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_987_length_3500_cov_1.930334"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLK68_07535"
     CDS             <1..323
                     /locus_tag="PLK68_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90298.1"
                     /translation="KICISYTNFQDQLDFIKSADSNTLIVIFSFSGNYVMKNGYSRFY
                     HTYDYLKSSKAKVYVITKNNLVQELEYVNEVILVPIKHDLYNYTLQCLVDLIFMKYQK
                     NLIK"
     gene            392..1255
                     /locus_tag="PLK68_07540"
     CDS             392..1255
                     /locus_tag="PLK68_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRM90299.1"
                     /translation="MKINENCLPCLISQVIKVANITNIKNRDMFYRGVFQYLGKLNFT
                     KTNPEIIGATFEMIKQQVNDEDPYFELRKYYNELFLSRSTEFENKINSFETAVKYAII
                     GNIIDFSPIYNTQIKDIDKWFENIDQLKLAINQLEEMITDIKSAKVLLYLGDNCGEIC
                     LDKLLIRRIKKLNPEIDIYFGVRGKPVVNDSIEADAYFVGMDEYATIISNGDNSLGTV
                     LERTSNEFKRIYRSADIVIAKGQANFESLSEQEKNIYFLLMVKCEVIANYIGVAQKSL
                     ICLNYYKSMSH"
     gene            1399..>3500
                     /locus_tag="PLK68_07545"
     CDS             1399..>3500
                     /locus_tag="PLK68_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HRM90300.1"
                     /translation="MKKIVLGILAHVDAGKTTLTESILYLTGTTRKLGRVDHRDTFLD
                     YDFQERNRGITIFSKQAIINWHDSQITLIDTPGHIDFSTEMERTLQVLDYAILVISGI
                     DGVQGHSETIWNLLRYYKIPTFIFINKMDVSRYEQSELMQNLKERLDEHCFDFSNLNN
                     EFYESVALNSEDLLNYYLEYNTLNKEMLINEIARCQLFPCFFGSALKMERIDNFFNEF
                     TNYIKNKDYSESFGARVFKISHDEQGNKLTHLKITGGSLKVKTQLLNDEKVDQIRIYS
                     GNKYHLTDEVMAGDICAIKGLKSIVAGQGLGIEDNTTQPLLSPYMDYQIKLPPDCDQH
                     QVLKKLNLLAQEDPQLHINYNVRTKEIHVQLMGEIQIEVLKNIIQERFKVEVDFDYGR
                     IIYKETIEETVEGVGHFEPLRHYAEVHLLLEPGEPGSGLKFDTNCQEYLLANNYQRLI
                     LSHLQEKEHLGVLTGSPITDMKITLVAGKAHLKHTEGGDFREATYRAIRQGLKTAKSV
                     LLEPYFDFSLELPIEYLSRAIYDIEAMAGNFKLPENQTDIAVITGSAPVSKMQNYQSE
                     VVRYTKGKGRLICQLSDYRPCQNQAEVIKSFNYDSEADVENPTGSVFCSHGAGYNVRW
                     DQVAQHMHIPFVFKKIKKTANNIQDKSKFDNIDDELENIFTRTYGPVKQRSGEHQVTK
                     KIFNESSYKYIPECLLVDG"
BASE COUNT         1299 a          403 c          641 g         1157 t
ORIGIN      
        1 gaaaaatttg catttcttac actaactttc aagaccaact agattttatt aaaagtgcag
       61 atagtaatac tttaattgta atttttagct tttctggtaa ctatgtcatg aaaaatggct
      121 attcacgttt ttatcatact tatgattatt taaaaagtag taaagcaaag gtttacgtaa
      181 ttactaaaaa taatttagtg caggagctcg aatatgtcaa tgaagttatt ttggttccaa
      241 taaaacatga tttatataac tatactttgc aatgtcttgt tgatttgatt tttatgaaat
      301 atcaaaaaaa tttgataaaa tagaatttcc attttttgga aataatattt atttaaatat
      361 aagaatgcta tgctaaagga gggtgagtcg aatgaaaata aatgaaaatt gtttaccatg
      421 cttgatcagt caagttatta aagtggccaa tattacaaat ataaaaaatc gtgatatgtt
      481 ttatcgagga gtatttcagt atttgggaaa actaaatttt actaaaacta atccagagat
      541 aattggtgct acatttgaaa tgatcaagca gcaagttaat gatgaggatc catattttga
      601 acttcggaaa tattataacg aacttttctt atcacgtagt actgaatttg aaaataagat
      661 caactctttt gaaacggctg ttaaatatgc aattattgga aatattattg attttagtcc
      721 tatctataat acccaaatta aagacattga taagtggttt gaaaatattg atcaattaaa
      781 attagcaatt aatcagcttg aagaaatgat aactgatata aaaagtgcaa aggtactttt
      841 atatttaggt gataattgtg gtgaaatttg tcttgataaa ttattaatta gaagaattaa
      901 aaaactcaat ccggagattg atatatattt tggtgtaaga ggaaaaccag tagtaaatga
      961 ttcaattgaa gctgatgcat attttgttgg aatggatgaa tacgctacaa ttatcagtaa
     1021 tggtgataat tctttgggta cggtattaga acgtacgagt aatgaattta aaaggatata
     1081 tcgaagtgcc gatatagtta ttgctaaagg gcaggcaaac tttgaaagtt tgagtgaaca
     1141 agagaaaaat atttattttt tattgatggt taaatgtgaa gttattgcta attatattgg
     1201 ggtcgcacaa aaatcattaa tttgtttaaa ctattataaa agcatgagtc attgattcat
     1261 gcttttacaa caattcaaaa taattttagt tgaattaagc atatttttaa gctgaattag
     1321 ataaattggt aatatcaatt gtaattatag taatattctt tataattata ttattagaaa
     1381 ataagtagga tgatataaat gaaaaagata gtattaggaa ttcttgcaca tgttgatgca
     1441 ggaaaaacaa cattaactga gagtatatta tatttaacag gaacaacacg aaaattaggt
     1501 cgagttgatc atcgggatac ttttttagat tatgattttc aagagagaaa tcgagggatt
     1561 acaatttttt ctaagcaggc aataataaat tggcatgata gccaaattac tttaattgat
     1621 acgccaggtc acattgattt ttcgacagaa atggaacgta cgctacaagt gctcgattat
     1681 gctattttgg taatcagtgg gatagacggt gtccaaggtc atagtgaaac tatttggaat
     1741 ttattgagat actataaaat accaaccttt atttttatta ataaaatgga tgttagtcgt
     1801 tatgagcaat ctgaattaat gcagaattta aaagaacgac tagatgaaca ttgttttgat
     1861 tttagtaatt tgaataatga attttatgaa agtgttgctt taaatagcga agatttattg
     1921 aattattatc tagaatataa taccttaaac aaagagatgt taattaatga aattgctaga
     1981 tgccaattat ttccgtgttt ttttggctct gcgttaaaaa tggaacggat tgataatttt
     2041 tttaatgaat ttactaatta tataaagaat aaggattatt ctgaatcttt tggagcgcga
     2101 gtttttaaaa taagtcatga cgaacaagga aacaaattga cacatttaaa aattactggc
     2161 ggtagtttaa aagtgaaaac gcaactttta aatgatgaaa aagttgatca aattcgtatt
     2221 tattctggga ataagtatca tttaactgat gaagtgatgg ctggggatat ttgtgcgatt
     2281 aaagggctta aaagtatagt tgctggtcag ggattaggta tagaagataa tacgactcag
     2341 ccattgttgt caccatacat ggattatcaa attaaattgc ctcctgattg tgatcaacat
     2401 caagtgttaa aaaagttaaa tttattagca caagaagatc ctcagctaca tatcaattat
     2461 aacgttagaa ccaaagaaat tcatgttcaa ttaatgggtg aaattcaaat tgaggtgctt
     2521 aaaaatataa tccaagagcg gtttaaagtt gaagttgatt ttgattatgg aagaattatt
     2581 tataaagaga cgattgaaga aactgttgaa ggggtaggac attttgaacc attacgtcat
     2641 tatgctgaag tacatttact tttagaaccc ggggaaccag ggtcgggatt aaaattcgat
     2701 acaaattgtc aagaatatct tctagcgaat aactatcaac gattaatttt gtcgcattta
     2761 caagaaaaag agcatttggg agtacttacg ggctcgccaa tcacagatat gaaaataact
     2821 ttagtggctg gaaaagcaca tttaaagcat actgaaggcg gtgattttag agaggcaaca
     2881 tatcgggcga tacgtcaagg cttgaaaaca gcaaaatcgg ttttactaga gccgtatttt
     2941 gatttttcac ttgaattacc gattgaatat ctgagtcgag cgatttatga tattgaggca
     3001 atggcaggga actttaaatt accggaaaat caaactgaca ttgctgttat taccggaagt
     3061 gcaccagtca gtaaaatgca aaattatcaa agtgaagttg ttagatatac taaaggaaaa
     3121 gggcgcctaa tttgtcagtt aagtgattat cgcccatgtc aaaatcaggc tgaagtaatt
     3181 aaatcattca actatgatag tgaggcagat gtagaaaatc caacaggatc agtattttgc
     3241 agtcatggtg ctggttataa tgtaaggtgg gatcaagttg cccaacatat gcacatacca
     3301 tttgttttta aaaaaataaa gaagaccgct aataatattc aggataaatc taaatttgat
     3361 aatattgatg acgagttaga aaatattttc acacgcactt atggaccagt taaacaaaga
     3421 tctggcgaac atcaagtaac taaaaaaata tttaatgagt caagttataa atacattcca
     3481 gaatgtttat tagttgatgg
//
LOCUS       DAOMTA010000246         3491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_990_length_3491_cov_2.758731, whole genome shotgun sequence.
ACCESSION   DAOMTA010000246 DAOMTA010000000
VERSION     DAOMTA010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_990_length_3491_cov_2.758731"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLK68_07550"
     CDS             <1..208
                     /locus_tag="PLK68_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90301.1"
                     /translation="NMVGTTMRLKIFNGPCKKLLITQHGALLESDFLISLNTDNDYYE
                     GQYVFMFYLDMIKIRYYELYIKEN"
     gene            213..953
                     /locus_tag="PLK68_07555"
     CDS             213..953
                     /locus_tag="PLK68_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90302.1"
                     /translation="MILGKVMMAQSANKRGSVEYQISKKTLMLGNRIKDYSLEMFIKE
                     AGVSKTSLMRYLNSLGVNTFTSFREIITLESIKGITALETIKYNYKEEQLDKQAVKLG
                     KIIKEAKRVIVLGDGNCFSLMIYMKAFIHLGIDFEIPIYLGKEEDIFDEYALSKDDLV
                     IFISLHETFRSFLDHRTMFYKDVRYFQFNCPCRVAFVGEVDLGQEPLSEYQFIVDIDG
                     LIYERKMRLDKLFEEAMSYLCLSYEFVY"
     gene            1025..1726
                     /locus_tag="PLK68_07560"
     CDS             1025..1726
                     /locus_tag="PLK68_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90303.1"
                     /translation="MLETKSTKKFKIIKGIICILTLAMLVAAVIFLFIVDDMKKKRRL
                     IFSIVQLLLMIGIIILPEQLKERLDLKIPIMLETSLTVFAFCGFVLGDVFDFYKKIPI
                     WDSILHAFSGVILAYAGFVLIDYFVKRESVNISMGHMFICTSVVLFSLALGALWEIGE
                     YLVDDIFGTNNQQYMKSTRGTLYGQKDVPLEGHAALGDTMKDLMLDLAGATAIVTIEY
                     CKEDYRKKKAKKEDK"
     gene            1799..2281
                     /gene="ispF"
                     /locus_tag="PLK68_07565"
     CDS             1799..2281
                     /gene="ispF"
                     /locus_tag="PLK68_07565"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRM90304.1"
                     /translation="MYRIGQSIDIHQLVAGRKLILGGVEIEHERGLLGHSDADVLCHA
                     VIESIIGALGLGDIGKHFSDTDAQYKGISSLVLLEKTYEMMDDRGYEISNLDAIILIE
                     KPKMAPHIQKMKENISRILKCDIADVNIKATRGEKLGFVGHEEGAVSQCVVMLKKKGI
                     "
     gene            2284..>3491
                     /gene="gltX"
                     /locus_tag="PLK68_07570"
     CDS             2284..>3491
                     /gene="gltX"
                     /locus_tag="PLK68_07570"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRM90305.1"
                     /translation="MEKVRVRYAPSPTGHLHIGGARTALFNYLFAKHHGGDFIFRLED
                     TDIERNIEGGEASQLENLAWLGIIPDESPLNPKPEYAPYRQMERLDIYRKYTQELLDR
                     GLAYKCFCTPEELDAEHERQVEAGVAPMYNRKCRDLTAEEVAAKEAAGIPYTIRLRVP
                     ANKTYEFDDMIRGHVSFESNDIGDWVIVKANGIPTYNYAVVIDDHTMEITHVFRGEEH
                     LSNTPKQMMVFEMLGWKAPTYGHMTLIVNEQRKKLSKRDESIMQFVSQYKEEGYLPDA
                     MFNFMALLGWSPEGEQEIFTKEELIKEFSETRLSKSPSMFDKDKLTWVNNRYIKERSL
                     EDVVALCRPFLEEAYDLSSKSEQWINDLVATYHDQLSYGKEIVSLVDLFFTDELNLDE
                     EAKEFMKDET"
BASE COUNT         1201 a          382 c          748 g         1160 t
ORIGIN      
        1 gaatatggta gggacaacaa tgcgtttaaa aatctttaat ggtccttgta agaagttatt
       61 aattactcaa cacggggcat tattagagag tgatttttta atttcattaa atactgataa
      121 tgattattat gaggggcagt atgtttttat gttttatcta gatatgatta aaataagata
      181 ttatgaatta tatataaagg agaactaggc agatgatttt ggggaaggtt atgatggctc
      241 aaagtgctaa taagcgtggg agtgtagaat atcagatcag taaaaaaaca ttgatgctcg
      301 gaaatcgaat caaagattat tcattagaaa tgtttattaa agaagctggg gtatcaaaaa
      361 catcattgat gcggtattta aatagcctgg gggttaatac ctttacttct tttagggaaa
      421 taatcacatt ggagagtatt aaaggcatca cagccttgga aacaataaaa tataactata
      481 aggaagaaca gctagacaaa caggctgtta aattaggaaa aataattaaa gaggctaaac
      541 gtgttattgt tttgggggat ggtaattgtt ttagtttgat gatttatatg aaagccttta
      601 ttcatctagg gatagatttt gaaattccta tttatttagg taaggaggag gatatttttg
      661 atgaatatgc attatctaaa gatgatctgg taatttttat cagtcttcat gaaacgttta
      721 gaagtttttt agatcatcga acgatgtttt ataaggatgt tagatatttt caattcaatt
      781 gtccgtgtcg agttgcattt gtgggagaag tagatttagg acaagaacca ctgagtgaat
      841 accagtttat cgtagatatt gatgggctta tatatgaacg taaaatgcgt ttagataagt
      901 tatttgaaga ggcaatgagt tatttatgtc ttagttatga atttgtttat taattcttaa
      961 gtttttattg atgggaattt ttgtttgttt ttgttataat acagtatatt atggaggcgg
     1021 taatatgctt gaaactaaat caactaagaa atttaagata attaaaggaa taatttgtat
     1081 tcttacttta gcaatgctag tggcagcagt gattttttta ttcattgttg atgatatgaa
     1141 aaagaagcga cgtctgattt tttcaattgt tcaattattg ttaatgatag gaattataat
     1201 tttaccagaa cagttaaagg aacgacttga tttaaaaatt ccgattatgt tagaaacctc
     1261 actaactgta tttgcttttt gtggatttgt acttggtgat gtgtttgatt tttataaaaa
     1321 aatacctatt tgggactcaa tactacatgc tttttcaggt gtgatcttag cttatgcagg
     1381 ttttgtttta attgattatt ttgttaaacg tgaaagtgta aatatatcga tgggacatat
     1441 gtttatttgc actagtgtag tattgttctc gttagcattg ggtgctttat gggaaattgg
     1501 agagtatttg gttgatgata tttttgggac taataatcag cagtatatga agtctacgcg
     1561 aggaacttta tatggccaaa aagatgtgcc tttagaagga catgcagctt taggcgatac
     1621 gatgaaagat ttaatgttag acttagctgg agcaacagct attgtaacaa tagaatactg
     1681 taaagaggat tatcgcaaaa agaaagctaa gaaagaagat aagtaaactt tgagcattga
     1741 aaatgcccta gaatataggg cgtttttgtg ttagaatatg gcgtggagga gtgagagtat
     1801 gtatcgaatt ggacaatcaa ttgatattca tcagctagta gcaggacgaa agttgatttt
     1861 gggtggtgtt gaaatcgaac atgagcgtgg tttgttagga catagtgatg ctgatgtttt
     1921 atgtcatgcc gttattgaaa gtattattgg ggctttagga ttaggtgata ttgggaaaca
     1981 ttttagtgac actgatgctc agtataaagg gattagttct ttagtgctat tagaaaaaac
     2041 ttatgaaatg atggatgatc gcggttacga aataagtaat cttgatgcaa tcattttgat
     2101 tgaaaaacca aagatggcac ctcatatcca aaaaatgaag gaaaatatta gcaggatctt
     2161 aaaatgtgat attgctgatg ttaatattaa agcaacccgt ggtgagaagc ttggctttgt
     2221 tggtcatgaa gaaggtgctg tttctcaatg tgtggttatg ttaaagaaga aagggattta
     2281 agaatggaaa aagtaagagt aagatatgct cctagtccaa ctggacatct acatattggg
     2341 ggagcaagaa ccgctttatt taattatcta tttgcaaaac atcatggtgg agattttatt
     2401 tttaggttag aggatactga tattgaaaga aatattgaag gtggtgaggc tagccaatta
     2461 gaaaatctag cgtggttagg aattattcca gatgaatcac cattaaatcc aaaaccagag
     2521 tatgcgccat atcgtcaaat ggaacgtttg gatatttatc gtaaatatac tcaagagtta
     2581 ttagatcgag gtttagcata taaatgcttc tgcaccccag aggaattaga tgctgaacat
     2641 gaacgccaag ttgaagctgg tgttgcacca atgtataatc gtaaatgtcg tgatttaaca
     2701 gctgaagaag tagcagctaa agaagcagca ggaattcctt atacaattag acttcgagtg
     2761 cctgcaaata agacatatga gtttgatgat atgatccgtg gacatgtatc atttgaatcg
     2821 aatgatattg gtgattgggt aattgttaaa gccaatggga ttccaactta taactatgca
     2881 gttgtaattg atgatcatac catggaaata acacatgttt ttagaggtga agaacattta
     2941 agtaatacgc caaaacaaat gatggttttt gaaatgttag gatggaaagc accgacttat
     3001 ggacatatga ctttaattgt aaatgaacaa agaaagaaat tatcaaaacg tgatgaaagt
     3061 atcatgcaat ttgtttcaca atacaaagaa gaaggatatt taccagatgc aatgtttaac
     3121 tttatggctc tattagggtg gtcaccagaa ggagaacaag aaattttcac aaaagaagaa
     3181 ttaattaaag aatttagtga aacgagatta tctaaatcac cttcaatgtt tgataaagat
     3241 aagttaacat gggtcaataa tcgttatatt aaagaaagat cattagaaga tgttgttgca
     3301 ttatgtcgac catttttaga agaggcttat gatctatcaa gtaaatcaga acaatggatc
     3361 aatgatttag ttgcaacgta tcatgatcaa ttatcttatg gtaaagaaat tgttagttta
     3421 gtcgatttat tctttacaga tgaattgaat ttagatgaag aagctaaaga atttatgaaa
     3481 gatgaaacta t
//
LOCUS       DAOMTA010000247         3490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_991_length_3490_cov_2.312373, whole genome shotgun sequence.
ACCESSION   DAOMTA010000247 DAOMTA010000000
VERSION     DAOMTA010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_991_length_3490_cov_2.312373"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLK68_07575"
     CDS             complement(<1..1120)
                     /locus_tag="PLK68_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90306.1"
                     /translation="MSKKEKNSFVDKIMGPMDKISSPLIKFGQIPFIQGLQRGMVSSI
                     GVTMVGSIFLVICLFGADGNITEKALLPFLTPYIDQLSLINSLSMNIMAIYMCVAMGA
                     EYADIKGINKTTGAVGALFAFILLNYNGMAATSEGVNALEITYWGSAGIVTAIIAMAI
                     SVNVIHLCYKYNIRIKLPSSVPPAISDSFSSIVPYLFVALICWSIRTIMGFDIPAVIT
                     NILLPVLSGADNIFVFCFAYFFASLCWVCGIHGDNIVGTVISPMLQTWMIENTEAYTA
                     GLAAPHIWIDQLNRLFQYVSTCWPILIYMYMSSKKLPQLKPLAVLSTPSMIFCIVEPL
                     MFGLPIVLNPFLAIPFVLIHTITAAVSYLLTSIGFVGRF"
     gene            complement(1872..2468)
                     /locus_tag="PLK68_07580"
     CDS             complement(1872..2468)
                     /locus_tag="PLK68_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90307.1"
                     /translation="MRKIMFTFLLTLSMIMLINIVPTKAFDNETELDLLIRRIDHINE
                     IYNSNYYILSEEEFINSEITDTFKSYDDYVKHLLNYDLNELENELIVNITVDSETINV
                     NIETYFRSTSGSRTLSFYNGNNKMILKYKYSGSKFDTSYKPGVTVTKVNTKNFFEMSS
                     HTGSFKNSNKTYSIIAKGRVITPSGVVSNKSFTVNFNL"
     gene            complement(2470..2766)
                     /locus_tag="PLK68_07585"
     CDS             complement(2470..2766)
                     /locus_tag="PLK68_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM90308.1"
                     /translation="MIKGRETFKIFESNIRLKMVAGLSKSNLTYNQLRELCNCSDGNI
                     ATHTKKLVSAGFIDVKKEFVNNKPKTTYNLTEYGQKEFKEYIKFLKKIIEETEK"
BASE COUNT         1302 a          540 c          419 g         1229 t
ORIGIN      
        1 caaatcttcc tacgaaacca attgaggtta acaaataact tactgcagca gtaatagtat
       61 gaattaacac aaatggaatt gctaagaaag gatttaatac aataggtaac ccaaacatca
      121 aaggttccac aatacagaaa atcattgatg gtgttgataa cacagctagc ggttttaatt
      181 gtggtaactt tttagaactc atatacatgt agattaagat cggccaacaa gtagatacat
      241 attggaataa tctatttaat tgatcaatcc atatatgagg tgctgctaaa ccagctgtat
      301 atgcttctgt attttcaatc atccaagttt gtaacattgg agagataact gtccccacaa
      361 tattatcacc atgaatacca catacccaac ataatgaggc aaagaaatat gcaaaacaga
      421 aaacaaagat gttatccgca ccggaaagaa ctggtaataa aatatttgtg attactgcag
      481 gaatgtcgaa tcccataata gtacgaattg accaacaaat taaagcaaca aataaatatg
      541 gcacaataga tgaaaaacta tctgaaattg ccggtggtac actacttggt aatttgatcc
      601 taatgttata tttgtaacat aaatgaataa catttacaga tatcgccatc gcaataatag
      661 cagtaacaat ccccgcacta ccccaatatg taatttctaa tgcatttaca ccttcagaag
      721 ttgctgccat accgttatag tttaataaaa tgaacgcaaa taacgctcct acagcaccag
      781 tagttttatt aataccctta atatcagcat attcagctcc cattgccaca cacatataaa
      841 ttgccatgat attcattgac aaactattaa ttaatgacaa ttggtcaata tatggtgtta
      901 aaaacggtaa taatgctttt tctgtaatat ttccatcagc tccaaataaa caaataacca
      961 aaaagattga tccaaccatt gtaacaccaa ttgacgaaac cattcctctt tgtaaacctt
     1021 gaataaatgg aatttgacca aatttgatta acggactgga aattttatcc ataggtccca
     1081 taatcttatc tacaaaagaa tttttttcct ttttagacat gatactctcc tcctcttcaa
     1141 tcgaaaaaat aagcgctttc ttttttctaa aatcattata gattttaatg tctattttat
     1201 caatttttct aaacgagctt atatgtgccg ttttattaca tgtctatatt cactcttatt
     1261 tatgaaaaaa ctgtaacaca ccaattgctc atcttattgg taatattaca gttttataat
     1321 catttatatt ttttaatttt gtaataatct ttttataact tttttcttta ccaaacaacg
     1381 tactagttta taaaacaaac cctttgacac ttacctttga taacatcgca atttctccgg
     1441 gaaataagta ctgagaaaaa atagaagatg ctcacaaagg tatttcttga tcaacttctt
     1501 taaaacagac taaatggttt gctgaaagat acaaaaacgt agatgataga tgcagtatat
     1561 tagtaaaagc aaagatcaat aaaaaatata tactatcatc ttttgaggac aaagatgaag
     1621 tagtaattga ctacaaaaga ttttttgatt tagaattctt aaaataaaca gtataaaaaa
     1681 taattgttat aatatttagg aaaataagta tccgtcccaa agttctcatt tctcaatcgt
     1741 gtatagaagg acatgtagat gaataaagaa atacgcgatg ttctttattc atcttaccag
     1801 agaagtttat atacaaaaag cctgttaact gaagtgaact aaaaaacact ttagttaaca
     1861 ggtttttatt cttataaatt aaagtttact gtaaaagatt tatttgatac aactcctgaa
     1921 ggagttatga ctcttccctt tgcaataatg ctatatgttt tattagaatt cttaaatgac
     1981 ccagtatgac tactcatttc aaagaagttt ttagtattta ccttagtaac agttacccct
     2041 ggtttatagc ttgtatcaaa tttactacca ctatatttat attttaatat cattttatta
     2101 tttccgttat aaaatgataa agttctactt cctgaagttg aacgaaaata tgtttcgata
     2161 tttacattaa tagtttcact atcaactgta atattgacta ttaattcatt ttctagttcg
     2221 tttaagtcat aatttaataa atgtttaaca taatcatcat aagacttgaa agtatccgta
     2281 atttctgagt taataaactc ttcctcagat aaaatgtaat aattggaatt ataaatttca
     2341 ttaatatggt caatccgtct tattaataag tctaactcag tttcattatc aaaagctttt
     2401 gtcggaacta tattaattaa cattatcatt gatagtgtta aaaggaatgt aaacattatt
     2461 tttctcattt tatttctccg tttcttcaat aatttttttt aaaaatttaa tgtactcctt
     2521 aaattccttt tgcccatatt ctgtcaaatt gtaagttgtt tttggtttat tgttgacaaa
     2581 ttcttttttt acatctataa aaccagcaga aactagtttt ttagtatgtg ttgctatatt
     2641 accatcacta caattgcaaa gctctcgtag ctgattataa gtcaaattag attttgataa
     2701 tcctgcaacc atttttagcc taatatttga ttcaaaaatt ttaaaggttt ctcttccttt
     2761 tatcatccgt atccctcttt agaaaaataa tagtaaataa cataataatg gctacaagtc
     2821 catatgtatc aaaatcataa aatatattca taaaaacacc aaaagaatta gaaatagctc
     2881 ttgaactata aatgtaagcc ttaagaataa aagatacaac aatataacat ataaacaata
     2941 aagttataat cattgttctc ttgtttctaa agtaggaata aatataaaat aattcaatta
     3001 aaactataat ataggacaaa gaaatagttt gataaaaaga tatcattgca tcagtgttta
     3061 gatagtttgt tagtatcggg ataattattt ctatggaaaa caaaataggg aaaataatcc
     3121 ataatttcaa aaacctacgc tcttttaccg atagttcttt taaacatatt gttttccaaa
     3181 tatataaatt tatgcatata ttaaataata cttttccccc attatacaaa ttgtagaaat
     3241 atttgtagtt ataaaaacca aatttaaagt ttaagatatc gattacatat ataagtaaca
     3301 tcattgaaga ataacatata agccatttac aaaacacact aattattcca cgatatggtg
     3361 aatactccgt tgtaatatac tctgtatctt taatagcctg atttattatt tttaatttat
     3421 taatatcgtt atttttgctc attagtataa cctcgattat atataaggat attataactt
     3481 attttatata
//
LOCUS       DAOMTA010000248         3485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_995_length_3485_cov_2.013120, whole genome shotgun sequence.
ACCESSION   DAOMTA010000248 DAOMTA010000000
VERSION     DAOMTA010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_995_length_3485_cov_2.013120"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..219)
                     /locus_tag="PLK68_07590"
     CDS             complement(49..219)
                     /locus_tag="PLK68_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM90309.1"
                     /translation="MAYVIGDDCIACGTCIDECPSGAISEGEKYSINPDMCTECGTCA
                     DVCPSEAISLPK"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            399..1148
                     /locus_tag="PLK68_07595"
     CDS             399..1148
                     /locus_tag="PLK68_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRM90310.1"
                     /translation="MKRKVLCLTLIMFLLALRINAQERLVENIPYADLKTFHFGVVIG
                     THVQDTQFKNIGEQTIANESGIGIKKNIVCDQDRWDPGFTVGVAGEFKLNNYLGLRIS
                     PTMYFGSRHLTFRNRTDLDINNNYTETYQDLKSIYASAAIDLIYNSQRFNNQRPYILV
                     GINPMLNLSAKNDDYIKLKTYDYLFEIGIGCDFYMPLFKLKPELKFCYSLINSLDTKH
                     ANDLKDKSMLLYTNSVKESRTKMIVLSFYFE"
     gene            complement(1136..1975)
                     /locus_tag="PLK68_07600"
     CDS             complement(1136..1975)
                     /locus_tag="PLK68_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HRM90311.1"
                     /translation="MKAKYILLTCLFFVAFSSFGKKRQLIILHTNDTHSCILPIPADI
                     GDTLFADRGGYIRRSTMIAEQRKSNPNLLLFDSGDFSQGSPFYTMFKGDVEIELMNIM
                     KYDAATIGNHEFDFGLDNMARVFKKASFPIVCSNYDVTGTVLGDCVKPYIIIKRGGLK
                     IGVMGLSPQLEGLVFKINYKGITYKDPGIVALQMATFLKEKKKCDVVICLSHLGWDIG
                     GQDDQYMISHSRNIDLVLGGHSHSYLKKLEYVKNIDGKPVPVDQNGKNAVFVGKLTID
                     YSK"
     gene            complement(1975..2745)
                     /locus_tag="PLK68_07605"
     CDS             complement(1975..2745)
                     /locus_tag="PLK68_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRM90312.1"
                     /translation="MIMNKKSLFIGLGFLLLTSYSCSTYHLLEIKRSLVIVDKRYDEK
                     PDSVALSFLRPYKQHVDSIMTPIVGQISENMSGYRPESPLSNLLADILLWAGGTYNEK
                     PDFAVYNIGGIRSSLVKGNVTYGDVLDVAPFENKICFLTLKGTDVIELFRNIASSGGE
                     GVSHGVNLVITKNGNLVSCELNGKQIDENRFYRVSTIDYLAQGNDKMLAFKSGLYLNS
                     PKSNRNDTRYIIADFFRQKMKEGIVIESKIEGRIKVNK"
     gene            2933..3286
                     /gene="rplS"
                     /locus_tag="PLK68_07610"
     CDS             2933..3286
                     /gene="rplS"
                     /locus_tag="PLK68_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRM90313.1"
                     /translation="MDLIKVAEEAFATGKNFPEFKSGDTITVAYKIIEGTKERIQLYR
                     GVVIKICGHGNKRRFTVRKMSGTIGVERIFPIESPSIDNIEVNKHGKVRRAKLYYLRK
                     LTGKKARIQEKRQVK"
BASE COUNT         1243 a          614 c          523 g         1095 t
ORIGIN      
        1 aaaaggcgac ttaaaagtcg ccatttatat ttaataaata atcttcaatt atttcggaag
       61 actaattgcc tctgaaggac aaacatctgc acaagtacca cactctgtac acatatccgg
      121 attaattgaa tatttttctc cctcagaaat agctcctgat ggacattcgt caatacatgt
      181 accgcaagcg atacaatcat caccaattac gtaagccata ataattaaat gttttttgtt
      241 aataatgttt tgtacttagt tttatgtacg atgcaaagtt acattaatat ttctaaatac
      301 ctataannnn nnnnnntagg tatatatttt ttaatatttt aagttttgtc aaaattagaa
      361 agatacaata agaaatcaag tataacgtta tatgtattat gaaaagaaaa gtattatgct
      421 taactctaat aatgttccta ctagcattaa gaataaatgc acaagaaagg ctagtagaga
      481 atatacctta cgcagacttg aagacattcc attttggcgt agtaatcggt acgcacgtac
      541 aagatacaca atttaaaaat ataggagaac agacaatcgc taatgaatca ggaattggca
      601 taaaaaaaaa tattgtatgt gaccaagaca gatgggatcc tggatttaca gtaggtgtag
      661 ctggcgaatt taaacttaac aactatctcg gattaagaat atccccaact atgtattttg
      721 gctcaagaca cttaacattc aggaatagga cagatttaga tattaataat aattacactg
      781 aaacatatca ggatttaaaa agcatatacg cttcagcggc aatagatctt atatacaatt
      841 cacagagatt caacaatcaa aggccatata ttcttgtagg tattaatccg atgctcaatt
      901 tatcagcaaa gaatgatgac tatatcaaac taaagacata tgactatcta ttcgaaattg
      961 gaattggctg tgacttttac atgccgttat ttaaattaaa acctgaacta aaattctgtt
     1021 acagtttaat aaacagttta gatacaaagc atgctaatga tttgaaagac aaaagcatgc
     1081 tattgtatac aaattcggtg aaagaatcaa gaacaaaaat gattgtacta tccttctact
     1141 tcgagtaatc aatagtgagc ttacctacga aaactgcatt ttttccattt tgatcaacag
     1201 gaacaggctt accatcaata tttttcacat attcgagttt cttcaaataa gaatgtgaat
     1261 gaccccctag taccaaatca atattacgag aatgtgatat catatactga tcatcttgtc
     1321 caccaatatc ccaacctaag tgtgaaagac aaataacaac atcacacttt ttcttttctt
     1381 ttagaaatgt agccatttgc aatgcgacta ttccaggatc tttataagtt atccctttat
     1441 aattaatttt aaatacaagt ccctctagtt gtggggaaag tcccattact ccgattttca
     1501 agccaccacg cttaataata atgtaaggtt ttacgcagtc accaagcaca gtacctgtaa
     1561 catcataatt agaacataca ataggaaaag acgctttttt gaatacacga gccatattgt
     1621 caagtccaaa atcaaattca tgatttccta ttgttgcggc atcatacttc ataatattca
     1681 tcaattcaat ttcgacatcg cctttaaaca ttgtatagaa aggagagcct tgcgagaaat
     1741 caccgctatc aaataataaa aggttcggat tagattttcg ttgttccgct atcatagttg
     1801 agcgcctgat ataaccgcct ctatctgcga ataatgtatc tccaatatct gctggtattg
     1861 gcaatataca actatgagta tcatttgtgt gtagaattat cagttgtctt ttttttccaa
     1921 atgaagaaaa agcaacaaaa aaaagacatg ttaataatat atattttgct ttcattattt
     1981 atttaccttt atgcgacctt caatttttga ttcaattaca ataccctctt tcattttctg
     2041 cctaaagaaa tctgcaataa tatatcgagt atcgtttcta ttcgattttg gtgaatttaa
     2101 atataatcca ctcttaaatg ccaacatttt atcgttcccc tgggctaaat aatctattgt
     2161 tgaaactcga taaaaacggt tctcatctat ctgctttcca ttaagttcac aagaaacaag
     2221 atttccgttt tttgttataa ccagattaac accatgacta accccttctc cacctgatga
     2281 ggcaatattt ctaaataatt caatgacatc agtacctttt aatgtaagaa aacatatttt
     2341 attctcaaaa ggagcgacat ccaaaacatc accatacgtt acattgcctt taacaagaga
     2401 agatcttatt cctcctatat tatatacagc aaaatcaggt ttttcattat atgtaccacc
     2461 agcccataat agtatatctg ctaaaagatt ggataacgga ctttccggtc tataaccaga
     2521 catattctcg gaaatttgtc caactatagg agtcattata ctgtccacat gttgtttata
     2581 tggacgtaaa aacgataatg caacagaatc aggcttttcg tcataacgtt tgtcaactat
     2641 cactaaggat ctctttatct ctaataaatg ataggtagaa caagaatagc tggtgagcaa
     2701 taggaatcct aatcctataa ataaagattt tttattcata atcataaagt ttggcacaaa
     2761 gttagttaaa aaaagaagaa aataagtcag tttagcaaaa aaaacaattt ggttcttgta
     2821 aaagaaaaga aaaattagta cctttgcacc gcttttcggc gtaatcgctg gtaggataag
     2881 agttacctac tatgttgcgt tggaacgata aacaaattta attataaata agatggattt
     2941 aattaaagtt gctgaagaag catttgcaac cggcaagaac ttccctgaat tcaaaagtgg
     3001 tgatactatc actgtcgctt acaaaattat cgagggtact aaagaaagaa tacagctcta
     3061 tcgtggtgtt gtaattaaga tttgtggaca tggaaacaaa agacgtttca ctgttcgtaa
     3121 aatgtcagga acaataggtg ttgagcgtat tttccctatc gaatcaccga gcatcgataa
     3181 cattgaagtt aacaagcatg gtaaagtacg tagggccaaa ttgtactact tacgtaaact
     3241 aacaggtaaa aaggctcgta ttcaagaaaa aagacaagtc aaataagcct aagccaagta
     3301 gactttatga aataaaaaga ggttccacat ggaacctctt tttatttata tacaatgctt
     3361 tattttttcg gaatctgatc caatttttct ttttcgtccc cctcccaatt tacctgtgca
     3421 tctgtatcgc cataaagagt ttccttaaaa ctgtcccaat cttgaaagcc agcaaataaa
     3481 gaaag
//
LOCUS       DAOMTA010000249         3478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1000_length_3478_cov_2.413380, whole genome shotgun sequence.
ACCESSION   DAOMTA010000249 DAOMTA010000000
VERSION     DAOMTA010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_3478_cov_2.413380"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1536
                     /locus_tag="PLK68_07615"
     CDS             76..1536
                     /locus_tag="PLK68_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HRM90314.1"
                     /translation="MKIGFDNNKYLTMQSSHIKERINQFGDKLYLEFGGKLFDDYHAS
                     RVLPGFAPDSKLQMLLQLKDQAEVVIAISASDIERNKVRGDLGITYDNDVLRLIEIYR
                     SKGLYVGSVVITQYDGQKAADIFKSRLEKRNIAVYLHYLIDGYPTNTKRIISEEGFGK
                     NDYIETTKPLVVVTAPGPGSGKMATCLSQLYHEHIRGIKAGYAKFETFPIWNLALNHP
                     VNLAYEAATADLNDVNMIDPFHLEAYNKVTVNYNRDIEIFPVLKNMFEEIYGTSPYNS
                     PTDMGVNMVGFCMSDDEACKEASRQEIIRRYYNTMNRYVREECSLDEVNKQELVMNQA
                     KVVVEDRKCVAAANQKSKEANCYAGAIELDDGTIITGRTSDFMGSCSAILINAIKYLA
                     NISDDEHLISPTAFGPIQNLKTEYLGSVNPRLHSDEILIALSLSAVNNDKAKLALQQL
                     PKLQGTQAHITSSIGPMDAKVFSNLGIQVTYDAKKL"
     gene            1956..2783
                     /locus_tag="PLK68_07620"
     CDS             1956..2783
                     /locus_tag="PLK68_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90315.1"
                     /translation="MNIEIANRLVRLRKEKNLSQEALANELGISRQAVSKWERAEASP
                     DTDNLILLAKLYGMSLDDLLKTDQKEFESGNNQQASKEKTEEDKKQKKKESVHISLKH
                     GIHVTGEDGEEVHVGWNGIHVEDPKEDSRIHIDGSGVFVDDKKYDQEEWRKIRKEKGW
                     DYYYEDYTKFPFALLAIIAYIGIALYTELWHPLWIFLLIVPIIEGAISAVKHRNLNRF
                     PYPVLVILYFLYEGFYENIWSPTWLIFLTIPVYYSLVNYFRQRKQHKNNNCTDNQED"
     gene            3020..3454
                     /locus_tag="PLK68_07625"
     CDS             3020..3454
                     /locus_tag="PLK68_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM90316.1"
                     /translation="MKELLLKRLKKAKNKKGFTLIEIIVVLVILGILAAIAVPAVMGY
                     IDDAKESKYVAEAHSIYIVIQTEEARAKATAADDITYESIEKEAIKKTSLEDVTIAPT
                     PTNVTPKEEKATSYKVTWTSDDDKKIEATVKKNKDVKIDEVN"
BASE COUNT         1317 a          432 c          658 g         1071 t
ORIGIN      
        1 gatttaatgc aaagtcgtgc aatcatttgt taaataaagt agaatatttt agataaaata
       61 tatttggagg atagtatgaa gataggattt gataataata aatatttaac gatgcaatca
      121 tcgcatatta aagaaagaat taatcaattt ggtgataaat tgtatcttga atttggtggt
      181 aaattgtttg acgactatca tgcttcaaga gttttaccag ggtttgcccc agatagtaag
      241 ttgcagatgt tattacaatt aaaagatcaa gcagaagtag ttattgcaat ttctgccagt
      301 gatattgaac gtaataaggt tcggggcgat ttaggtatta cttatgataa tgatgtttta
      361 cgtctaattg aaatatatcg atcaaaggga ctatatgtgg gaagtgtagt aatcactcag
      421 tatgatggtc aaaaagcagc ggatattttt aaaagccgat tggaaaaaag aaatattgcc
      481 gtgtacttac attatttgat tgatggttat ccaactaata ctaaaagaat cattagcgag
      541 gaagggtttg gaaaaaatga ttatattgaa actactaagc cgctggtagt tgtaacagct
      601 ccgggcccag gaagtggtaa aatggcaaca tgtttatcac aactatacca tgaacatatt
      661 cgcggtatta aagctgggta tgcaaaattt gaaacattcc caatttggaa tttagcatta
      721 aatcatccag ttaacttagc ctatgaagct gctactgctg atttaaatga tgtaaatatg
      781 atcgatcctt ttcatcttga agcatacaat aaagtaactg ttaattataa tcgtgatatt
      841 gaaatcttcc cagtattaaa gaatatgttt gaagaaatat atggaactag tccatataac
      901 tcaccaactg acatgggtgt taatatggta ggtttttgta tgagtgatga cgaagcttgt
      961 aaggaagcta gtcgccaaga aataattcgt cgttactata atacaatgaa tcgctatgtt
     1021 cgtgaagagt gttcattaga cgaagttaat aagcaagaac ttgttatgaa tcaggcaaag
     1081 gttgtagttg aagatcgtaa gtgtgttgcg gctgcaaacc aaaagtcgaa agaagctaat
     1141 tgttatgcag gagcaatcga attagatgat ggaacaatca ttacaggtcg tacatctgat
     1201 ttcatgggat catgttctgc aattttaatc aatgctatta aatatttagc taatatcagt
     1261 gatgatgaac atttaatttc accaacagcc tttggtccaa ttcaaaattt aaagactgaa
     1321 tatttaggaa gtgttaatcc acgtttacat agtgatgaaa tattgattgc attatcatta
     1381 agtgctgtta ataatgacaa agctaagtta gcgctacaac aattacctaa actacagggg
     1441 acccaagcgc acattacttc ttctatcgga ccaatggatg ctaaagtttt tagtaacctt
     1501 ggaattcaag taacgtatga tgctaaaaaa ttgtaatgta aaaatatcaa aaatgatatg
     1561 atgacctcct aatagacagt gtaaataatt aaacactgac taggaggttt ttatattttg
     1621 ataagacaaa aggattttta gaaatcatac tattaataat acttgtatag tttaaaaaat
     1681 ggttttttta gtaaaagaaa atggactgcg caagcacgga acagtccatt aaccagaaag
     1741 gaggtgtttt acaccataca aatgtatcac ctaactttat gataatcatt atttgaaaat
     1801 atttcaacaa atattataat ttatgtgtat tcatcgaaca aattataata taatggtcat
     1861 attgcaacca ctggttgcga aattgaaaac tagaatatct agagcaacca agcattattt
     1921 tgcataatgt aggtgattta gggaggaata aatttatgaa tatagaaatt gcaaatcgat
     1981 tagtaagatt gcgaaaggaa aaaaatcttt cacaagaggc tttagcaaat gaattaggaa
     2041 tcagtcgcca agcagttagt aagtgggaaa gagcagaagc atctccagat acagataatt
     2101 taatcttact tgccaagtta tatggaatgt cattggatga tttactaaaa acagatcaaa
     2161 aagaatttga aagtggtaat aatcaacaag catcaaaaga aaaaacagaa gaagataaga
     2221 aacaaaagaa aaaagaaagt gttcatatta gtttaaaaca tgggattcat gtgactggtg
     2281 aagatggtga agaggttcat gttggctgga atggaattca tgttgaggat ccaaaagaag
     2341 attcaagaat tcatatcgat ggttctggtg tgtttgtgga tgataaaaaa tatgatcaag
     2401 aagaatggcg taaaattcgt aaagaaaaag gctgggatta ctactatgaa gattatacaa
     2461 agtttccttt tgcattactt gcaattattg cttatatcgg gatagcctta tacacggagt
     2521 tatggcatcc attatggata tttttattaa ttgttccaat tattgaaggg gcaatttctg
     2581 cagttaaaca ccgcaacctt aacagattcc cgtatcctgt attagtaatt ttatattttt
     2641 tgtatgaggg attctatgaa aatatttggt caccaacctg gttaattttt ttaacgatac
     2701 ctgtttatta ttcacttgtt aattatttta gacaacgaaa acaacataaa aataataatt
     2761 gtacagacaa tcaagaagat taataatgat atttatcacg ggttcatcac aaattatcga
     2821 aaaacattaa aatagttgaa ttatgtgtga ttagtctaag atattaattg ttattacctt
     2881 cgggtatata atgagactgt agtaaaaagt tatgttaaga aagaaaagaa tcggtattgc
     2941 atgggaccag gaggtaatac cggttttact ttgttaagaa ctttttagac attcattgat
     3001 gataaacgga ggagaaaaaa tgaaagaatt attattaaaa agattgaaaa aagctaaaaa
     3061 taagaaaggt tttacgctta ttgaaatcat cgtagtatta gtaattttgg ggattttagc
     3121 tgcgattgct gttccagcgg taatgggata tattgatgat gctaaagaat caaaatatgt
     3181 tgcagaagca cattcaattt atattgttat acaaacagaa gaagcacgag ctaaagcaac
     3241 tgctgctgat gatataactt atgaaagtat agagaaagaa gcaattaaga aaacaagttt
     3301 agaagatgta acaattgcgc cgacacctac taatgtaaca ccaaaagaag aaaaagctac
     3361 gtcttacaaa gttacatgga ctagtgatga tgataagaaa atagaagcaa cagttaagaa
     3421 aaataaagat gttaaaattg atgaagtaaa ttaaacatta gaataaatta tagaataa
//
LOCUS       DAOMTA010000250         3477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1002_length_3477_cov_2.772355, whole genome shotgun sequence.
ACCESSION   DAOMTA010000250 DAOMTA010000000
VERSION     DAOMTA010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_3477_cov_2.772355"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..822)
                     /gene="map"
                     /locus_tag="PLK68_07630"
     CDS             complement(76..822)
                     /gene="map"
                     /locus_tag="PLK68_07630"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRM90317.1"
                     /translation="MIVTKDRREIELMAEAGRIVALVHEKLKEVIVPGITTKQIDEIC
                     EKVIRDNNATPSFLHLYGFPNSVCTSINEVVVHGIPSDRKLEEGDIISVDVGACYKGY
                     HGDSAWTYAVGKISDEAKRLMEVCEASLYAGLEQVKPGNRLSDISHAVQVYLEDHGCT
                     TPLDYTGHGIGTEVHEDPAVPNYGQAGRGPRLKAGMTLAIEPMAHLGGCETEVLQDDW
                     TVVTKDRSLAAHYEHTIVITDDGYEILTKL"
     gene            complement(822..1469)
                     /locus_tag="PLK68_07635"
     CDS             complement(822..1469)
                     /locus_tag="PLK68_07635"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRM90318.1"
                     /translation="MNIILMGPPGAGKGTQAANLVKEYGLTHISTGDIFRKALKEQTK
                     YGVIAKYFMQFGHLVPDDYTIQMVREYLQENEFPNGFILDGFPRTIIQARELESIAKE
                     FGFEIDAVINLDIELDRLVPRLSGRRTCKECGASYHIEYNPPKVEGVCDVCGGELYQR
                     PDESEDAVKVRLDTYEKQTSPLIDYYTMKGQITNINGDQSMEDVFKDIKASLEVK"
     gene            complement(1493..2794)
                     /gene="secY"
                     /locus_tag="PLK68_07640"
     CDS             complement(1493..2794)
                     /gene="secY"
                     /locus_tag="PLK68_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRM90319.1"
                     /translation="MFTFLKNVFKKGELRQKVIFTLGILFVYRLGAAITIPSVNTGAL
                     SSSDATNGIFGIMNLLGGGTLERFSLFSLGVSPYITSSIIIELLSMDVIPALSQWRKE
                     GNTGKKKKDRVTRYVTLFLAALQGGVLTYAFDNGYKILADSSIWTYIYVVLIMMAGSM
                     LAVWLGDQITNKGIGNGVSLLIFTGIVSNLPSSFISTFNNLVDTSGKAGDMWLGIAWY
                     ALFVVVYLSIVIFVVFNEGAVRKIPIIYATSSNTTMRTKDQTHMPIKINSSGVIPVIF
                     ASSVLAAPRTVVSFMETSDVTKWINTIFNYQEPVGFVLYILMIIGFTFFYSNLQIDAE
                     KISDDLKKNGGAIPGVRTGVDTMKHIKTILNRVTVVGSLSLCIIAAIPIITPVIWSQT
                     ANASLSLGGTGLIIVTGVALETVKQIKTHITRKEYHGYIRR"
     gene            complement(2796..3236)
                     /gene="rplO"
                     /locus_tag="PLK68_07645"
     CDS             complement(2796..3236)
                     /gene="rplO"
                     /locus_tag="PLK68_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRM90320.1"
                     /translation="MKLHELQYTEGARKTRKRVGRGTSSGTGKTAGRGQKGQGARSGG
                     GKKPGFEGGQTPLFMRLPKRGFTNFNKLEYAIVNLDQLNTFEAGTVVCPKALKEAGLI
                     KKELDGVKVLGNGTLEKAITVKAHKFSKSALAAIEAAGGKTEVI"
     gene            complement(3251..3433)
                     /gene="rpmD"
                     /locus_tag="PLK68_07650"
     CDS             complement(3251..3433)
                     /gene="rpmD"
                     /locus_tag="PLK68_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRM90321.1"
                     /translation="MAKTVMITLKKSPIGCLPKQRATLKALGLTKVNKTVEKENNEFI
                     QGMIKVVGHLIKVEEK"
BASE COUNT         1041 a          735 c          505 g         1196 t
ORIGIN      
        1 gtaatgtttc aagtaccgtt gcttctactt caagaacgtc atctttcttt gccataggtt
       61 ttcaactccc ttaaattaaa gttttgttaa gatttcatac ccgtcatcag taatgacgat
      121 cgtatgttca taatgagccg ctaaagagcg gtcttttgtt acaactgtcc aatcatcttg
      181 taatacttcc gtttcacatc cacctagatg tgccatcggt tcgattgcaa gtgtcatacc
      241 tgctttaagt cttggaccac gaccagcttg accataatta ggaacagcag gatcttcatg
      301 tacctcagta ccaatcccat gccctgtata atctaacggt gtagtacatc catgatcttc
      361 aaggtatact tgcacagcgt gagaaatatc tgataagcga ttaccaggtt taacctgttc
      421 taatccagcg tataatgaag cttcacaaac ttccatcagc cgtttagctt catcgctgat
      481 tttaccgact gcataagtcc acgcgctatc tccgtgatat cctttataac aagccccaac
      541 atcaactgaa atgatgtcac cttcttctaa ttttcgatca ctaggaattc catgtacgac
      601 tacttcatta attgaagtac aaacactgtt tggaaatcca tataagtgaa gaaatgaagg
      661 agttgcatta ttatcacgaa tcactttttc gcaaatctca tcaatttgtt ttgtcgtaat
      721 tcctgggaca ataacttctt taagcttttc atgcactaat gcaacaattc ttccagcttc
      781 agccatcaat tcaatttctc tacggtcttt agtaacaatc attatttaac ctccaggcta
      841 gctttgatat ccttaaagac gtcttccata gattgatcac catttatatt agtgatttgt
      901 cctttcattg tataataatc aattaaagga cttgtttgtt tttcataagt atccagtctg
      961 acttttacag catcttcgct ttcatcaggc cgttgataca actcaccacc acaaacatca
     1021 catacccctt caactttagg tggattatat tcgatgtggt aacttgcacc acattcttta
     1081 caagttcttc ttccagaaag tcttggtact aaacgatcta attcgatatc taaattgatt
     1141 acggcatcaa tttcaaatcc aaattcttta gcaatacttt ctaactcacg agcttgaata
     1201 atagtacgag ggaaaccatc aagaataaat ccatttggaa attcattttc ttgaagatat
     1261 tctctaacca tttgaatagt ataatcatca ggaactaagt gaccaaattg catgaagtat
     1321 tttgcaataa ctccatattt agtttgctcc ttaagtgctt ttctaaaaat gtctcctgtt
     1381 gaaatgtgag taagtccata ctctttcact aaattagctg cttgagtacc ttttcccgct
     1441 ccaggtggcc ccattagaat aatgttcata taattgcccc ttctttctta tactatcgac
     1501 gaatataacc gtgatattct tttcttgtaa tatgagtttt aatttgtttt acagtttcta
     1561 gtgcaacccc agtaacgatg attaaacctg taccaccaag tgataaagat gcgtttgctg
     1621 tttgtgacca gataactggt gtaataattg gaattgctgc aatgatacat aacgataatg
     1681 atcctaccac agttactctg tttaaaattg tcttaatgtg tttcattgta tcaactccag
     1741 ttctaactcc cggaattgct ccaccgtttt tctttaaatc atcactgatt ttttcagcat
     1801 caatttgtaa gttagaatag aagaatgtaa atccaataat catcaagata tataagacga
     1861 atccaactgg ttcttgataa ttaaagattg tattgatcca tttagtaaca tcgctagttt
     1921 ccataaaact aactactgtt cttggtgctg ctaatactga tgaagcaaag ataactggaa
     1981 taacccctga actattaatt ttaataggca tatgagtttg atctttagtt ctcattgttg
     2041 tattagagct tgttgcataa attattggta tttttctaac ggcaccttca ttgaaaacaa
     2101 caaagatcac gattgaaaga taaactacca caaataatgc ataccaggca attccaagcc
     2161 acatatcccc agctttacca gaagtatcaa ctaagttgtt gaaagttgaa ataaaactgc
     2221 tcggtaagtt tgaaacaatc ccagtaaaga ttaataatga taccccatta ccaatccctt
     2281 tatttgtaat ttgatcccct aaccatactg ctaacatact acctgccatc ataattaaga
     2341 caacatagat atatgtccag atactagaat ctgcaagaat cttgtatcca ttatcgaaag
     2401 cgtaagttaa tacgccacct tgtaatgctg ctaagaataa tgttacatat cttgtaaccc
     2461 ggtctttttt ctttttgccg gtattacctt ctttacgcca ttggctcaat gccggaataa
     2521 catccattga caataattca ataatgattg aagaagtgat gtaaggtgaa actcctagcg
     2581 agaatagaga gaatctctct aatgttcccc cacctagtaa gttcataatc ccaaaaatcc
     2641 cattagtggc atcactagaa cttaatgctc cagtgttgac actcggaata gtaattgcag
     2701 ctcccagacg ataaacaaat agaatcccca gagtgaaaat tactttttga cgaagttcac
     2761 cttttttgaa aacatttttc aaaaatgtaa acatcttaga tcacctcggt ttttccacct
     2821 gcagcttcga ttgcggcaag agcagattta gaaaacttat gagcttttac agtaatagct
     2881 ttttctaaag ttccattacc taatacttta acaccatcta attctttttt gattaaccca
     2941 gcttctttta atgctttagg acatacaaca gtccctgctt caaaagtatt taactgatct
     3001 aaattaacaa tagcgtattc taatttatta aagtttgtaa atccacgttt tggtaaacgc
     3061 atgaataatg gagtttgtcc cccttcgaat ccaggtttct ttccaccacc agatctagcg
     3121 ccttgccctt tttgtcctct accagcagtt ttaccagtac cagaacttgt accacgtcca
     3181 acacgttttc ttgttttacg agcaccttca gtgtattgta attcgtgtag tttcattagt
     3241 ttacacctcc ctatttttct tctactttaa ttaggtgtcc aacaactttg atcattcctt
     3301 gaatgaattc gttgttttct ttttctacgg ttttatttac ttttgttaaa cctaacgctt
     3361 taagagtagc ccgttgtttt ggtaaacaac caattggact ttttttaagc gtaatcataa
     3421 ctgtttttgc cattttttac gccctcctat ccaaaaattt cttctacttt tttatct
//
LOCUS       DAOMTA010000251         3473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1006_length_3473_cov_2.232007, whole genome shotgun sequence.
ACCESSION   DAOMTA010000251 DAOMTA010000000
VERSION     DAOMTA010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_3473_cov_2.232007"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..2909
                     /locus_tag="PLK68_07655"
     CDS             12..2909
                     /locus_tag="PLK68_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRM90322.1"
                     /translation="MKISKKRKQNKFTRKIKTLVLALAMIIPAAGTNLIVEAENNPGS
                     TTSSDGLVTVNKTSERVGDNRYKINLDLATGKAQDSVEAIGNDIVLVFDISNSMAEDE
                     HGNSTSSNDKKRLTKAKNAAIEFLNNSKISGNKKNRYSIVTFNYYGTVEQNLTSNLET
                     AKQAIRDVELGNNSDGGTNIQAGLYKARTVLKNAKSENGIIILLSDGGATGSYKLNNE
                     RNNGYLVNDYSEATATDKALGYSGRYTFGENAINYDSVIKGGRNDFTLDLYLNNSHYS
                     LNNAAATLAENEALLAKKSGNTIFTIGYTTGSSVNSFLKNVATQGEGYAYSSSSDLSG
                     IYENIANEIVTRYETIIKNGIVNDPMSQYVDLLDLPDGRYDNSVADKLIFDRGYVIVS
                     TENGRKKITWYVGDVEKNSQAHLEYYVAVKEEYKDGTDYPANDPTQLVYKNYIDKDST
                     LDFNVPTVNEELPITSATLTVKKVVNNSSEDKTFIIHVVGRDSENAVIYKTDLILKNN
                     EEVLLENLPFGKYTVTETVPMEYKLESTESGYAQTNLSSGNIIELSQNKDAANGYVTL
                     TNSFGHVGFFKATDEKTNYLPAGSETINNYEYNVSGNVKSTTVKAPQDVVLLLDKSGS
                     MDESMNGSSRLTHLKNNVIKFITKLYEHNPDSRVSVITFAYSADGSITNNNFVKLSDI
                     KSGNETWYTYLTKNNGGIKNIKASGGTQIDLGLYEVRNQLSSATGENNRSVIVFTDGQ
                     PGNKGFNTSYNDYDDNGYRVGAEALNQADFIKFSGNLTGINNYIESSNGSKYYGHKND
                     DITKNRSNNNSNDAGNRTNRSGKGLGKTIFTIGLNSNNSSLFDSFLTRLASEGHYTKA
                     NNSSAMENAFNSIFTSITTMDTDVDIRVKYDANKFEVIDAQGGKLGGSGTNAYIEWKD
                     IIDETTGKFVIEGIKFRNIVADAGEPQLTVQGYKDGVTTDLEAAEIKKVGN"
     gene            2933..>3473
                     /locus_tag="PLK68_07660"
     CDS             2933..>3473
                     /locus_tag="PLK68_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90323.1"
                     /translation="MKYLGRIKMKRPLMVLMVVVTVCLTAVGGIYAWSKLSASVTNNI
                     KTPTVDTEVVEKFDKDSTVDWFDQVEKEVQFKNTGTAPVFLRVSYAEFWKDADGKVIS
                     GLVDGNEVVNKNWTEAWQNDWFDGGDGWYYYKKVLPANEMTAKVLTSLKFDSNYAEIY
                     QTADYTLKFVSEAVQYSDDD"
BASE COUNT         1302 a          410 c          702 g         1059 t
ORIGIN      
        1 gaggaagcta aatgaagata tctaaaaaaa gaaaacaaaa taagtttact agaaagataa
       61 aaacactggt tttggcatta gcgatgataa taccagcggc tggaactaac ttaattgttg
      121 aagcggaaaa taaccctggc tctactacta gtagtgatgg cctagttact gttaataaaa
      181 cttcagaaag agttggcgat aatcggtata aaatcaattt ggatttagct actggaaaag
      241 cccaggatag tgttgaggcg attggtaatg atattgttct agtatttgat atttcaaata
      301 gtatggctga agatgaacat ggaaatagca catcttcaaa tgataaaaaa cgtcttacta
      361 aagctaaaaa tgctgcaatt gagtttttga ataatagtaa aatttctgga aataaaaaga
      421 atcgatattc aattgtgaca tttaattact atggtacagt agaacaaaat ctaacaagca
      481 atcttgaaac cgcaaaacaa gcaattagag atgtcgaatt aggaaataat tcggatggtg
      541 gaaccaacat tcaagcaggg ctttataaag ctagaactgt tttaaaaaat gctaaatcag
      601 aaaatggaat tattatttta ttaagtgatg gtggtgcgac aggaagttat aaactaaata
      661 atgaacgcaa taatggttat ttagttaatg attatagcga agcgaccgct actgataaag
      721 ctttaggata tagtggtaga tatacttttg gggaaaatgc aatcaattat gatagtgtaa
      781 ttaagggcgg aagaaatgat tttacattag acttatattt aaataatagt cactattctt
      841 taaataatgc agctgctaca ttagctgaaa atgaagcatt attagcaaag aaaagtggaa
      901 atacaatttt tacgattggt tacacaactg gaagtagtgt taatagtttc ttgaagaatg
      961 tagctactca aggtgaagga tatgcctata gttcctctag tgatttaagc ggaatttatg
     1021 aaaatattgc taatgagatt gtgactcgat atgaaacaat cataaaaaat gggattgtca
     1081 atgatccaat gagtcagtat gttgatcttt tagatttacc tgatggccgt tatgataact
     1141 ctgtagctga taaattaata tttgatcgtg gttatgttat tgtaagtaca gaaaatggtc
     1201 gaaaaaaaat cacttggtat gtaggtgatg ttgaaaaaaa cagtcaagct catttagaat
     1261 attatgtagc agttaaagag gaatataagg atggtacaga ttatccagcc aatgacccaa
     1321 cccagttagt ctataaaaat tatattgata aagattctac attggatttc aatgtgccaa
     1381 cagttaatga agaattgcca attacaagtg caactcttac tgtaaaaaag gttgtaaaca
     1441 attctagtga agataagaca tttattattc atgtggttgg tcgagatagt gaaaatgcag
     1501 taatttataa aacagattta atcttaaaaa ataatgaaga agttctttta gaaaatttac
     1561 cgtttggtaa atataccgta acagaaactg ttccaatgga atataagcta gaaagtacag
     1621 aaagtggtta tgctcaaaca aacttatcta gtggaaatat tatcgaactt agtcaaaata
     1681 aagatgcagc taatggttat gtgactctaa caaattcatt tggtcatgtt ggattcttta
     1741 aagcgactga tgaaaagact aattatcttc ctgctggaag tgaaacaatc aataactatg
     1801 aatataatgt aagtggaaat gttaaatcta cgacagtaaa agccccacaa gacgttgtct
     1861 tgctattaga taaatcaggc agcatggatg agtcaatgaa tggcagcagt cgtctaaccc
     1921 atttaaaaaa taatgtaatt aaatttataa ctaagttata tgaacataat cctgacagtc
     1981 gtgtatcagt aattactttt gcatatagtg cagatggttc gatcactaat aataattttg
     2041 tgaagttatc agatattaaa tcaggtaatg aaacatggta cacatattta actaaaaata
     2101 atggtggtat caaaaatatt aaagcttcag gaggaacgca aattgattta ggtttgtatg
     2161 aagttcgtaa tcaattatca agtgcaactg gagaaaataa tcgttcagta atcgtcttta
     2221 ctgacggtca accaggaaat aagggattta atactagtta taatgattat gatgataatg
     2281 ggtatcgggt tggagctgag gctttaaatc aagctgattt tattaagttt agtggaaatt
     2341 taacaggaat caataattac attgaaagta gtaatggatc aaagtattat ggtcataaaa
     2401 atgatgatat tactaaaaat cgtagtaaca ataattctaa tgatgcaggc aatagaacta
     2461 atcgttctgg taaaggttta ggtaagacga tttttacgat tggtcttaat tccaataatt
     2521 catcattatt tgatagtttc ttaacgcgtt tagctagtga aggacattat acaaaagcaa
     2581 ataacagtag tgcgatggaa aatgcattta atagtatttt tacaagtatt acaacaatgg
     2641 ataccgatgt tgatattcga gtaaaatatg atgctaataa gtttgaggta attgatgctc
     2701 aaggtggtaa gctaggtggc agtggtacta atgcttatat tgaatggaaa gatattattg
     2761 atgaaactac tggaaaattt gtaattgaag gaattaaatt tagaaatatt gtggctgatg
     2821 caggagaacc acagttaact gtgcaaggtt ataaggatgg tgtaactact gatttagaag
     2881 ctgcagaaat aaaaaaagtt ggtaattaat aaagaaggtg agggatgacg caatgaaata
     2941 tttaggtaga attaaaatga aacgaccatt gatggtattg atggtagtgg tgacagtctg
     3001 tcttactgca gtgggcggaa tctacgcatg gtcaaaacta agtgcatctg tcactaataa
     3061 tattaagaca ccaacagtgg atactgaagt agtagaaaag tttgacaaag attcaactgt
     3121 agactggttt gatcaagtag aaaaagaagt acaattcaaa aatactggaa cagcaccagt
     3181 ctttctaaga gtttcttatg cggagttttg gaaagatgct gatggcaagg ttatttctgg
     3241 gttagttgat ggcaatgaag ttgttaataa gaactggact gaagcatggc aaaatgactg
     3301 gtttgatggt ggtgatggct ggtattatta taaaaaagtt cttccagcta atgaaatgac
     3361 agctaaagtt ttaactagtc ttaaatttga tagcaattat gccgaaattt atcaaacagc
     3421 agattatact ttgaaatttg ttagtgaagc agtacagtat agtgatgacg ata
//
LOCUS       DAOMTA010000252         3465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1010_length_3465_cov_2.192375, whole genome shotgun sequence.
ACCESSION   DAOMTA010000252 DAOMTA010000000
VERSION     DAOMTA010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_3465_cov_2.192375"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..849
                     /gene="treR"
                     /locus_tag="PLK68_07665"
     CDS             136..849
                     /gene="treR"
                     /locus_tag="PLK68_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HRM90324.1"
                     /translation="MLSKYQRISQDLIDKIDKQEIKVHSYLPSENELMKLYNASRDTI
                     RKALDILLKNGYIQKNKGKGSLVLDVDKVAFPVSGVMSFKELAKTINSEVVTKVIELS
                     LNPVDVQMKKELYMDSGNYYKIRRIRSFDGERVILDTDYLNANIVPNITETIAADSLY
                     NYIENELGLKISFARKEFTVVKATEEEKNLLDMHEYDLLVCVKSYTYLEDASLFQYTI
                     SKHRPDKFRFVDFARRTQL"
     gene            873..>1268
                     /locus_tag="PLK68_07670"
     CDS             873..>1268
                     /locus_tag="PLK68_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90325.1"
                     /translation="MTQKDYLLFQDNIRKEKANLDSRSLKISIARLIIGLLIVGFLLG
                     GNFTNNQLLVYSSLIWIAIFIILIVIHSKITERLSYLNAQAIVIQRYLDRYNNDWKAF
                     DETGLDYVDNVTGVMKDLDLVGKNSLFQYF"
     assembly_gap    1269..1278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1279..1662
                     /locus_tag="PLK68_07675"
     CDS             <1279..1662
                     /locus_tag="PLK68_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90326.1"
                     /translation="ASTIRGKKCLLDKLTRTSFNEKGIIEEQKAVLELSNNNNFVLSL
                     ETYGRMLEKPKVNERIIEDFVNNLGKNKVKWSWGFAKYLIPILTIITIVMFLFEFYFS
                     NFSASANFWTTDYCNYKSKQSWNNF"
     gene            1592..2653
                     /locus_tag="PLK68_07680"
     CDS             1592..2653
                     /locus_tag="PLK68_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90327.1"
                     /translation="MPVLIFGQLIIAIINLNSHGIIFEQVSKLSHCLNNYQNISHLIK
                     QSTFTSPFLVELQARLEASSIAFDELKSLSSAVKQRNNLLAFILLNGLLLWDINCKER
                     YDRWVKQYASSIERWLDAIGEFEALTSLQVLLHTKDVTCFANFHGDQTTYLEFGEAYH
                     PLMPNEQAVANSFMMNHQVCVITGSNMSGKTTFLRTIGINLVLAYAGGPVMAKSFDCS
                     LMQIFTSMRIEDDLNGISSFYAELLRIKQIVEANRQGNLMIALIDEIFKGTNSKDRII
                     GAQETVKQLSTNNIFTFITTHDFELCELENEISCSNYHFNEYYQGDKIKFDYLIKYGR
                     SQTTNAQYLLKMVGITKEV"
     gene            2756..>3465
                     /locus_tag="PLK68_07685"
     CDS             2756..>3465
                     /locus_tag="PLK68_07685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HRM90328.1"
                     /translation="MGKFSSDASKLLEFVGGRENIRAVSHCVTRMRFVLGDESKADID
                     KIETLGSAKGTFTQGGQFQVIIGNDVQEFYNEFIKIAGIEGVSKEQVKSDAKSNQTWL
                     QRLMGNLGEIFAPLIPALICGGLILGFRNVIDSIAMFENGTKTLVQISQFWSGVDSFL
                     WLIGEAVFHFLPVGICWSITKKMGTSQILGIILGITLVSPQLLNAYSVASTAAKDIPV
                     WDFGFAQVQMIGYQAQVIP"
BASE COUNT         1217 a          382 c          651 g         1205 t
ORIGIN      
        1 ttacatttaa tcgttctcat caaacagtca agaatgttgc caaatattta gttgacggga
       61 tagatggatg tattgaagaa ttaattaagt gtcacaaata aatttatttg atataatgta
      121 tcagaggtgg ttaaggtgtt aagtaagtat caaagaatat ctcaggattt aatcgataaa
      181 atagataaac aagaaatcaa agtacattca tatttgccta gtgaaaatga gttaatgaag
      241 ctatataatg catctcgtga taccatccgt aaggctttgg atattttatt aaaaaatggt
      301 tatattcaaa aaaataaagg aaaaggatca ctagttttag atgttgataa agtcgcattt
      361 ccagtttctg gcgtaatgag ttttaaagaa ctagccaaga ctattaatag cgaggttgtt
      421 actaaagtta ttgaattatc tttaaaccca gttgatgttc aaatgaaaaa agaactatat
      481 atggatagtg gtaattatta taaaataaga cgaattcgtt catttgatgg tgagcgggta
      541 atattggata ccgattattt aaatgctaat attgttccta atataacaga aacaattgct
      601 gctgattcac tatataatta tattgaaaat gaattgggtt taaaaatcag ttttgcaaga
      661 aaagagttta cagttgttaa ggcgacagaa gaggaaaaga atttattaga tatgcatgaa
      721 tatgatctat tagtatgtgt gaaatcttat acatatttag aagatgcttc attgtttcaa
      781 tatacgattt cgaaacatcg tcctgataag tttagatttg ttgattttgc aagaaggact
      841 caactttaat gagttctttt ttaggagggg ctatgacaca aaaagattat ttattattcc
      901 aagacaatat tagaaaagaa aaagcgaatt tagatagtag gtcattaaaa atttcaattg
      961 ctcgtttaat tattggatta ttgattgttg gatttttatt aggaggcaat tttactaata
     1021 atcagttatt agtatattca tcattaattt ggattgctat ttttattatt ttaatagtta
     1081 ttcatagtaa aataacggaa cgattgagtt atttaaatgc tcaagcaatt gtaattcaac
     1141 gttatttaga tcgctataat aatgattgga aagcttttga tgaaacggga ctagactatg
     1201 ttgataatgt tactggggtg atgaaggatt tagatttagt gggaaagaat tcattgttcc
     1261 aatattttnn nnnnnnnngc aagtacgatt cgtggtaaga aatgtttgct tgataaatta
     1321 acgcgtacaa gttttaatga aaaaggtatt attgaagagc aaaaagctgt tttagaatta
     1381 tcgaataata ataattttgt tttatcattg gaaacctatg gcagaatgct tgaaaaacct
     1441 aaagttaatg aacgaattat tgaggatttt gttaataatt taggaaaaaa taaagttaaa
     1501 tggagctggg ggtttgctaa atatttaatt ccgatactta cgattattac cattgtcatg
     1561 tttcttttcg aattttattt cagtaatttt agtgccagtg ctaatttttg gacaactgat
     1621 tattgcaatt ataaatctaa acagtcatgg aataattttt gagcaagttt ctaaattatc
     1681 acattgcttg aataattatc aaaatattag tcatctgatt aaacaatcca cttttacatc
     1741 accttttttg gtagagttac aagcaagatt agaggcttca agtatagctt ttgatgaact
     1801 aaaaagtctt agtagtgctg ttaagcaacg gaataatctt ttggcattca ttttattaaa
     1861 tggacttttg ttatgggata tcaattgtaa agaacgatat gatcgttggg ttaagcaata
     1921 tgctagttca attgagcggt ggttagatgc aattggcgaa tttgaagctt taacgagttt
     1981 acaagtatta ctccatacta aagatgttac ttgttttgcg aattttcatg gtgatcaaac
     2041 tacatattta gaatttggtg aagcttatca tccattgatg ccaaatgaac aagctgttgc
     2101 taatagtttt atgatgaatc accaagtatg tgtaattaca ggctcaaata tgtctggaaa
     2161 aacgactttt ttaaggacga ttgggattaa ccttgtacta gcgtatgcag gcggaccagt
     2221 tatggcaaaa tcatttgatt gctcattgat gcaaattttt acttcgatga gaatagaaga
     2281 tgatcttaat gggatttcat cattttatgc tgaattatta agaataaaac aaatcgttga
     2341 agctaatcgt caaggtaatt tgatgattgc gttaattgat gaaattttta aaggtactaa
     2401 ttcaaaagat cgaatcattg gagcacaaga aacggtcaag caattgtcaa caaataatat
     2461 tttcacattt attacaactc atgattttga attatgtgaa ttagaaaatg agatttcatg
     2521 cagtaattat cattttaatg aatattatca aggagataag attaaattcg attatttaat
     2581 taaatatggt cgaagtcaaa caacaaatgc acaatattta ttgaagatgg ttggaattac
     2641 aaaggaagtt tagcttcctt ttttatttta ttaaaaatgt attgacttac atgtacgtac
     2701 aagttattat ataattacaa aagatgtacg tacatgtaaa aaaaggagaa aaaagatggg
     2761 aaaattcagt agtgatgcaa gtaaactttt agaatttgtt ggcggtagag aaaatattag
     2821 agcagttagt cattgtgtta ctagaatgcg ttttgtttta ggggatgaga gtaaagctga
     2881 tatagacaag attgaaacct tggggagtgc taaaggtaca tttacacaag gcggtcaatt
     2941 tcaagtgatt attggtaatg atgttcaaga attttataat gagtttatta agattgctgg
     3001 aattgaaggg gttagtaaag agcaagtaaa aagtgatgct aaatcaaatc aaacctggct
     3061 tcaaagatta atgggaaatt taggggaaat ctttgctcca ttaattcctg cattgatctg
     3121 tggagggttg attctaggat ttagaaatgt gattgattca attgcaatgt ttgaaaatgg
     3181 aacaaaaaca ttagttcaaa tttcacaatt ttggagtggt gtagactcat ttttatggtt
     3241 aattggtgaa gcagtattcc attttttacc tgttggtatt tgctggtcaa taactaaaaa
     3301 gatgggaact agtcaaattc taggtattat tttaggaatt acattagttt ctccacaatt
     3361 attaaatgct tattcagtag cttcaacagc agctaaggat ataccagtct gggattttgg
     3421 attcgcccaa gtacagatga ttgggtatca agcacaagtt attcc
//
LOCUS       DAOMTA010000253         3447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1017_length_3447_cov_2.598762, whole genome shotgun sequence.
ACCESSION   DAOMTA010000253 DAOMTA010000000
VERSION     DAOMTA010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1017_length_3447_cov_2.598762"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1484..1921
                     /locus_tag="PLK68_07690"
     CDS             1484..1921
                     /locus_tag="PLK68_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90329.1"
                     /translation="MRIIIILSLLGLTACNVSYGLKDTPISGVHSDFKLFVDEYCDIK
                     QEVLNLPCNDIPMQYGQVRDKNSNVIRLGECRYGSVKPHILINKDLPKGISLRQLIYH
                     EMIHCDLEYHKHDDSGLLNSYHQSWDNSNDMLELRKFFNNYKK"
     gene            1981..2148
                     /locus_tag="PLK68_07695"
     CDS             1981..2148
                     /locus_tag="PLK68_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90330.1"
                     /translation="MLTIDQIKIELLLNEKALRDALRDNNLMRLAELRMQRDNLKDMM
                     IEALNTKLEAA"
     gene            2347..2493
                     /locus_tag="PLK68_07700"
     CDS             2347..2493
                     /locus_tag="PLK68_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90331.1"
                     /translation="MSQTDRNILIKRLLRADKELTLELLNSMSNKTLVQLYKNICIIY
                     CFTG"
     gene            2502..2741
                     /locus_tag="PLK68_07705"
     CDS             2502..2741
                     /locus_tag="PLK68_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90332.1"
                     /translation="MDSLTYIKRRVNATYQFLLLPELSHLELACTVKTIKSLTKEESL
                     KLTHLIETVKHPDWTIVNRNADNLILKIKKYSKKY"
     gene            2789..3034
                     /locus_tag="PLK68_07710"
     CDS             2789..3034
                     /locus_tag="PLK68_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90333.1"
                     /translation="MKELFFTNKKLQNVTVLVEGNSFGDQIITAYYNRPDHSHFVIFT
                     QLNHEGQHETNMKALERQIVKSIPKVILPVGKMNWTV"
     gene            3106..3351
                     /locus_tag="PLK68_07715"
     CDS             3106..3351
                     /locus_tag="PLK68_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90334.1"
                     /translation="MKFRSFSCGRGMHRVFLFKSLFHVEVGWMTFNKLNNKYSIEIKR
                     VNFLIAYYEFYSHKTSEFFSETTNLTKRDFKHGNYIK"
BASE COUNT         1311 a          463 c          495 g         1178 t
ORIGIN      
        1 acatctaaca ttgaaaattt caaaattctt actgtcggta tgttattatt ttcccaactt
       61 agtttgtcct tgacccctat aacctcacca ctcaactatc actccagcct atacctacct
      121 atagcctagc aatagatcat tcaacctaga gcctattaga gcgtctcatt ataagaccat
      181 ctattcaata gcagactaac caagcttata aaagaatata agcccattac ataatccagt
      241 tctaatatgt tgcggcttaa cctatgtcct acaatcatgt tgtagtattt agattattat
      301 aacatattgc aagacctaga ccatcctgaa tcattctaac atggtccagc atttgcatat
      361 atacaaaaag tgtaccatcc tgaaaagaat aagcttaggc acaaagattg catattgaac
      421 tattttcagg aattaagtta gcatactaat tgcatataaa aataatcata caatctttac
      481 aggaataata taaaaaaaat aaatcaagac attgtatata taagaactaa tttaatccga
      541 caggattagt ttagttcgca atagtaaagt caatttgttt gattcattca aacaattgta
      601 tctttacgaa caagatcttt attaaataaa gccgataggc ttttttaaga cattaacgaa
      661 aagacgagat tctcaatata ggacgcttta actggagtaa agcttctaga tactcaatat
      721 gcaatgctat aagagtcttt tctcttatac aagctatagt aatagtaata tcatactttt
      781 tattaaaagt caagctattt attaatgata ataaatctca tttagagaca ttagtttagt
      841 actttcagga ataaaatagt gtaaatatat agaattatta tggttatata gatttaatat
      901 gtagctgcat ttaattttta ttagataagt atttaatttc attagggaat aattaattta
      961 atatgattta tttgttagta ggattgtaag ttgttgattt tattgaagaa aatgagagta
     1021 tcaattcaat ttacatatca cttacacatt atttacatat tatctacata tccatatcaa
     1081 tataatgcaa ataaacataa caatctacta tgtttagtat aagatctttt tatatagtcg
     1141 attatttaaa atagatctat tttaataacc acaagtaata aactaagtca atcaaaatat
     1201 taatattctc aatttaatat tttttactca aaaataaaaa tattaaagca tatataatat
     1261 tactatatta tataatatat tacatagtat gtttagaggt atttttttag gtattttctt
     1321 aaaaaacaac ttgcatattt tttaaaataa agctaaagta attatataac aaaggtagga
     1381 aatatggaca aaacaatctt cttcacattc ttaacgctag ttatgatcgg tttctctctt
     1441 tatgttgcaa tcactgaaac taagtaatta ggagactaat caaatgagaa taattatcat
     1501 cttatcatta ctaggattga cagcttgtaa cgtttcctat ggtttaaagg atactcctat
     1561 ctctggtgtt cattctgatt ttaaactctt tgtggacgag tattgtgata ttaaacagga
     1621 agtcttaaac ctaccttgta atgatatccc tatgcaatat ggtcaagtga gagacaaaaa
     1681 tagtaatgta atcagactgg gtgaatgtcg ttatggatcg gttaaacctc atattttaat
     1741 caataaagac ttacccaaag gtatatcctt aagacaatta atctaccatg aaatgattca
     1801 ttgtgatcta gaatatcata agcacgatga ttcaggactt ttaaactctt atcatcaatc
     1861 atgggataat tctaacgata tgctagaact aagaaaattt ttcaataatt ataaaaaata
     1921 attaaaaaag tcctaaagtt cctgtaaaat aagtcgataa gtaattaagt gaggtcaaaa
     1981 atgctgacaa tagatcaaat aaaaattgaa ttattactaa atgaaaaagc tttaagggat
     2041 gcattacgag acaataattt aatgagactc gcagaactta gaatgcaaag agataattta
     2101 aaagacatga tgatcgaagc gttaaacact aaactagagg cggcttaata tgattaaatt
     2161 aatgggcata aaaaataata tgtttcactg gctagtttca gtcaaagggt cttcaagtgt
     2221 ggacaagtta ggttgggaga ctttcgacaa gtttgatgag gcaaaaaaat attataatag
     2281 tttaaaaagt attaaagttt ccacaaaata aaccgataag gtatataaca aataagaggt
     2341 gtaatcatga gtcaaaccga tagaaatata ttaattaaaa gattgcttcg agcagataaa
     2401 gaattgacac tagaactatt aaacagtatg agcaataaaa ctctagtcca gctttataaa
     2461 aatatttgta ttatttactg ttttacagga taggattaat tatggatagc cttacttata
     2521 ttaaaagacg agtcaatgca acttatcaat tcttattatt accagaacta tcacacttgg
     2581 aacttgcttg caccgttaaa actattaaga gtctaactaa agaagaaagt ctaaaattga
     2641 cacacttaat tgagacggtt aagcatcccg actggactat agtcaatcga aatgcggaca
     2701 atttaatttt aaaaattaaa aaatattcaa aaaagtacta aagtattaaa aaattatacc
     2761 gataagtaat taacagggag ataaaagaat gaaagaatta ttttttacta ataaaaagct
     2821 tcaaaacgtg actgttttag ttgagggtaa tagttttggg gatcagatta ttacagctta
     2881 ttataaccgt ccagaccata gtcactttgt gatttttaca caattaaatc atgaggggca
     2941 gcacgaaact aatatgaaag ctttagagcg acaaatagtt aagtctatcc ctaaggtaat
     3001 actgccagtc ggcaaaatga attggacggt gtaatatgga aacgactttt attataatta
     3061 tgtttattat gtttgtaatt gcaatgtctt aacttgaggt ttatcgtgaa attccgttca
     3121 tttagctgcg gtcgtggtat gcatagggta tttttattta aatcattgtt tcatgttgag
     3181 gtagggtgga tgacatttaa taaacttaat aataaatatt ctatcgaaat aaaaagagtc
     3241 aattttttaa tagcttatta tgaattttat tctcataaaa ctagtgaatt ttttagtgaa
     3301 acaactaatt taactaagag agattttaaa catggaaact atattaaata attctgtttt
     3361 atttatcact tttttatgtc ctgttttatg tatcattttt agacttaata gagtgtatca
     3421 atatgataaa tttatgtttt ctctttc
//
LOCUS       DAOMTA010000254         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1029_length_3433_cov_1.987863, whole genome shotgun sequence.
ACCESSION   DAOMTA010000254 DAOMTA010000000
VERSION     DAOMTA010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_3433_cov_1.987863"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLK68_07720"
     CDS             <1..862
                     /locus_tag="PLK68_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM90335.1"
                     /translation="VGAKYFSCRQALSLEYDEIANLKKVLGNKKVWVLVNALVEEKYI
                     DFLEKHLIRLSHIGVDGILFQDFGVLQICNEHNFDFEMIYHPDTLNTNQATLNYLGTQ
                     GINGAFLAREIPLEEKKIIAKNVNVKTMIQVHGVEYMAYSKRKLLTNYFKEINQDIPI
                     GISDDLTILANGVNYSCHIYEDQYGCHILSKQQMCGLDIMSSFQDFDYLYIESLYVDE
                     LKLVEIVNLYQDALISVGNRTYGKVAKELISQLYQLDPNIEYHHSFMFDATVYKIDDV
                     RKREENEKCK"
     gene            846..2090
                     /locus_tag="PLK68_07725"
     CDS             846..2090
                     /locus_tag="PLK68_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM90336.1"
                     /translation="MRNVSKVINGKRVIIKKPELLAPAGNLEKLKVAIQYGADAVFVG
                     GKEFSLRSGASNFTLDDIKEAVTFADQYGAAVHVTCNIILHQDNLDGIEEYLRALDQA
                     GVRAIIVADPYIMSIAKKLNLKLEVHVSTQLSTLNTKAIKFYQNLKMDRVVLGREVCY
                     DDLKTILDKTDVDIEYFIHGAMCIHYSGRCMLSNYFSRRDANRGGCSQSCRWYYDLYQ
                     GNQKINQDGVIPFSMSSKDMALINHIPELIELGVDSFKIEGRMKSLHYIATIVSTYRK
                     LIDDYCNDPDNFELTEKYYREIQKAANRSLCTGFFDDQADNDKQLYNQRDEHPTQEFC
                     ARVISYDQVNQIATIEQRNYFKIGDQIEFFSPYHENIVVPVTKIINADNEEVEVANHP
                     MEILQIPVSVELQKDDMGRKVI"
     gene            2090..2710
                     /gene="udk"
                     /locus_tag="PLK68_07730"
     CDS             2090..2710
                     /gene="udk"
                     /locus_tag="PLK68_07730"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRM90337.1"
                     /translation="MKKPVIIGIAGGSASGKTSIAQELYDCFKGRHTIRIIKLDDYYK
                     DQTHLSMDKRVLTNYDHPLAFDMDLLIEHLDLLKEGKSIQKPTYDFEQHNRSKIVEIV
                     DCRDVFILEGLFVLNEVRIRERCDILVYVDTDADIRFIRRLRRDLEERGRSLDSVCTQ
                     YLTTVRPMHEQFVEPSKKYAHIIIPEGSSNTVAIDLLLTKISSIVD"
     gene            2775..3260
                     /gene="greA"
                     /locus_tag="PLK68_07735"
     CDS             2775..3260
                     /gene="greA"
                     /locus_tag="PLK68_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRM90338.1"
                     /translation="MEHEKVLLTQSGVEKLEQERDNLINVERPKVIEELQLARSQGDL
                     SENADYDAAREKQAHLESRIKEIDYMLQNAEVISEEQMDLKVVKPGTTVTILDLSEKD
                     AEPESYQIVGYTETDPLNGKISNESPLAKAVLGHGVNEIVTVGVADPYDVKIVNIEFK
                     N"
BASE COUNT         1241 a          425 c          658 g         1109 t
ORIGIN      
        1 cgttggggca aagtattttt catgtcgtca agcattatcg cttgagtatg atgaaatcgc
       61 caatttaaaa aaggtcctag gtaataaaaa agtatgggtt ttagtaaatg cactagttga
      121 agaaaaatat atcgatttct tagaaaagca tttaattaga ttgagtcaca ttggagttga
      181 tggaatttta tttcaagatt ttggtgtttt acagatttgt aatgagcata attttgattt
      241 tgagatgatt tatcatccag atacattaaa tactaatcaa gcaactttaa attacttggg
      301 aactcaaggt attaatggtg cttttttggc acgggagata ccgcttgaag aaaaaaaaat
      361 aattgctaaa aatgttaatg ttaaaacaat gattcaagtt catggtgttg aatatatggc
      421 atattcaaaa agaaagctat tgactaatta ttttaaggaa atcaatcagg atattccaat
      481 tggaatatca gatgatctga cgattctggc aaatggtgta aattatagtt gtcatattta
      541 tgaggatcaa tatgggtgtc atattttgag taaacagcaa atgtgtggct tagacataat
      601 gagtagtttt caagattttg attacctcta catagaatct ttatacgtcg atgaattaaa
      661 gttagtagaa atcgttaatt tatatcaaga tgctttaatc agcgttggaa atagaactta
      721 cggaaaagtc gctaaggaat taatatcgca actttatcaa ctggatccaa atatcgaata
      781 tcatcatagc tttatgtttg atgcaactgt ttataagatt gatgatgtaa gaaagcggga
      841 agaaaatgag aaatgtaagt aaagtaatta acggaaaaag ggtaataata aaaaaacctg
      901 agttattggc accagctggt aacttagaaa agctaaaggt tgccattcaa tatggtgctg
      961 atgcagtttt tgtgggtgga aaagaatttt cattacgttc tggagcctca aattttacac
     1021 ttgatgatat taaagaggca gtaacctttg ctgatcagta tggcgctgcg gttcatgtga
     1081 catgcaatat tattctacat caagataatc ttgatgggat tgaagaatat ctgcgggctc
     1141 ttgatcaagc aggtgttaga gcgattattg tggctgatcc atatattatg tcaattgcta
     1201 aaaagttgaa tttaaaatta gaagttcatg tttcaacaca attatcaact ttaaatacaa
     1261 aagcaattaa attttatcaa aatttaaaaa tggatcgagt agtgcttggt cgggaagttt
     1321 gttacgatga tttgaaaact attttagata aaactgatgt agatattgaa tattttattc
     1381 atggtgccat gtgtattcat tattcaggtc gttgcatgtt gtctaattat ttttcacgtc
     1441 gtgatgctaa tcggggtgga tgttcacaat catgtcgctg gtactatgat ctatatcaag
     1501 gtaatcaaaa aattaatcaa gatggtgtaa ttccttttag tatgtcaagt aaggatatgg
     1561 cattaattaa tcatattcct gaattgattg aactaggagt cgattctttt aaaattgagg
     1621 gtcgaatgaa gtcattgcat tatattgcaa caattgtttc gacctatcga aaattaattg
     1681 atgattattg taatgatcct gataattttg aattgactga gaaatattat cgtgaaatac
     1741 aaaaagcggc taatcgttcc ttatgcactg gtttctttga tgatcaggcg gataacgata
     1801 agcagttata taatcaacgt gatgaacatc ctacacaaga attttgtgcc cgagtaatca
     1861 gctatgatca agttaatcaa atagctacaa tagaacaacg taactatttt aaaattggtg
     1921 atcaaattga gttttttagc ccatatcacg aaaatattgt tgtgccagta acaaaaatta
     1981 taaatgctga taatgaagaa gttgaagtag ctaatcatcc aatggagatt ctacaaatac
     2041 cggtcagtgt tgaattacaa aaggatgata tgggaaggaa agtgatttga tgaaaaagcc
     2101 agttattatt ggtattgcgg gaggtagtgc ttcgggaaaa acctctattg cacaagagct
     2161 ttatgactgt tttaaaggac gccatacaat tagaattatt aaattagatg actattataa
     2221 agatcaaacc catttatcaa tggataaaag agtcttaact aactatgatc accccttagc
     2281 ttttgatatg gatttattga ttgaacatct tgatttatta aaagaaggga aaagtattca
     2341 aaaaccgact tatgattttg aacagcataa tcgtagcaaa atagtagaaa tagttgattg
     2401 tcgtgatgtt tttattcttg aagggttatt tgtgttaaat gaagtacgaa ttagagaacg
     2461 ctgtgatatt ctagtatatg ttgatacaga tgcagatatt cgctttattc gtcggttgag
     2521 acgcgatctt gaagaacgag ggcggagtct agattcggta tgtacacaat atttaacaac
     2581 agtaagacca atgcacgaac aatttgttga accatcgaaa aaatatgctc atattataat
     2641 tcccgaagga agcagcaaca ctgtagcaat tgatttattg ttaacaaaaa tatcttcaat
     2701 agttgattag tgaagatatt aggactagat atttatatag aattatgcta tattagagac
     2761 tacgaggtga tattatggaa cacgaaaaag tattgttaac ccaaagtggg gtagaaaaat
     2821 tagaacaaga aagagataac ttgattaatg tcgaaagacc taaagttatt gaagaattac
     2881 aattagcacg ttctcaaggt gacttatctg aaaatgctga ctacgatgct gctagagaaa
     2941 aacaagcaca tttagaatca agaattaaag aaattgacta tatgcttcaa aatgctgaag
     3001 ttatttctga agaacaaatg gatctaaaag tagtaaaacc cggtactact gtaacaatct
     3061 tagatttatc agaaaaagat gctgaacctg aaagttacca aatcgttgga tatactgaaa
     3121 cagacccttt aaatggaaag atttcaaatg aatctccatt agcaaaagct gttttaggtc
     3181 atggagtaaa tgaaattgta acagttgggg ttgcagatcc ttatgatgta aaaattgtta
     3241 acattgagtt taaaaactag aacttataaa atgaacctac tatttagact gcaaaataag
     3301 taaggttctt ttttataaac aattttaata agtagaaata gaaaaagaat gatttaatca
     3361 gtattttgta agaactgttt aaatcattct ttaagggaaa ttatctttgg aaagatgaaa
     3421 tattctatgg att
//
LOCUS       DAOMTA010000255         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1033_length_3416_cov_2.180303, whole genome shotgun sequence.
ACCESSION   DAOMTA010000255 DAOMTA010000000
VERSION     DAOMTA010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1033_length_3416_cov_2.180303"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..770)
                     /locus_tag="PLK68_07740"
     CDS             complement(477..770)
                     /locus_tag="PLK68_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRM90339.1"
                     /translation="MKFKPFDNKVWLSSPTMHGEELEYVKEAYETNWMSTVGVNINEV
                     EKLTCEKIGSKYAVALSAGTAALHLAMKLAGIKVYGMPKVGYGALEGKKGIAV"
     gene            complement(981..1481)
                     /locus_tag="PLK68_07745"
     CDS             complement(981..1481)
                     /locus_tag="PLK68_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRM90340.1"
                     /translation="MKDNWYVLFALVAKENKLCSVLRRKGLDAFIPKIEYYRRDIKGN
                     TLKMLFPGYIFVRSEMKQSDFDNLLYELGEQRDGLIKQLKDDGVTALRDEEIEMFNKL
                     LNSEGILEMSQAFIEDNKAKVIYGPLIHYQDHIVKIDKHNRIAVLDIEFLNRHILAGL
                     EIKSKI"
     gene            complement(1490..1996)
                     /locus_tag="PLK68_07750"
     CDS             complement(1490..1996)
                     /locus_tag="PLK68_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRM90341.1"
                     /translation="MNWYIIFANDNKINDLLFYFNNHPEMTAFVPKIEKLMKKDGKKV
                     FAEVPMFPNYIFIESEFNSQEFYQIIESLEKDMDSTMRIMQSDEQKVLSLANNEKELL
                     ESLFNDDHLITRSMGTITDSKLIVQKGPLVGKEEMIKKIDRHKRVAFIGDVFGKTMKV
                     PLEVTSKT"
     gene            2259..3392
                     /locus_tag="PLK68_07755"
     CDS             2259..3392
                     /locus_tag="PLK68_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM90342.1"
                     /translation="MPRRGENIFRRKDGRWEARYVKAISTNNKKLYGSVYASTYTEVK
                     QKRENICNSLNKEKIKKSNINNQVLEYYIVEWLNVYRIKFKAPTYVKYYNVIYNYLLP
                     YFKDIKANELNTKQVELFTASLLKEGKNGKPLSPKTVKDILSLFKSIIGYIESEHNIS
                     INTKIIYPKKQKEKERILSKEEFINLNQTLLQEIDSCKFGVLIAMATGLRIGELCALK
                     WKDISLKENYIYVNKTMQRIKNIYSDNPKTIIIEDSPKSSTSNRKIPIPANLITLFKQ
                     FRQNDDCYLLTGRSDKFVEPRSLERKFRKYIKNAGIEKANFHMLRHTFATMCIEGGFE
                     IKCLSEILGHSGSQITLDRYVHSSFDLKKSWIDKFSNQNISCH"
BASE COUNT         1238 a          589 c          411 g         1178 t
ORIGIN      
        1 tttcaatttc atttatgttt gcgccaactg ttgacatcca atttgtatga taagcttctg
       61 taatatattg cagttcttct ccatgcatag taggactact cagccatatc ttattttcaa
      121 atctttcaaa ttgatgattt tctgcattaa acatatccat gcacctctcg ttcattgtaa
      181 agtgtttatt tttaatctgt gttatatttt atacagatat taatgtcatt ttttaatttg
      241 tgtaggcaaa aaaagaccac tagctcaccg tgagcacatt cacgctgagc ctggtcaatt
      301 acctccactt attttttcta ctctatattg tgggtcaatt aagagtaccc aagaagccat
      361 ctcaacctct ccggttctcg aatctaactc gcaatccata gaaaattaca taatcagggc
      421 ttgacctagg ccctcgaaac ccgcataaaa tcaaggtttt ctcttggttg tccctattat
      481 actgctatcc ctttctttcc ttctaacgcg ccatatccaa cctttggcat accatatact
      541 ttgatacccg ccagtttcat ggctaaatga agtgcagcag tccccgctga taaagcaact
      601 gcatatttac ttccaatctt ttcacaggta agtttttcta cttcattgat atttacccca
      661 acagtagaca tccagttagt ttcataagcc tcttttacgt attctaattc ttcaccatgc
      721 attgttggtg aagataacca taccttattg tcaaaaggtt taaatttcat attttttcat
      781 ctccctcatc aaaataattt gagtaaccaa aaaagaactc tttatagaaa gagtcctaat
      841 cttgatatta atttcccctc cataataaaa aactgcttta tgcagttaaa ttgtacaaaa
      901 aaagaatata gttcccccta ccccaagtag aagaaactat attcaaatta ttaaaatgtg
      961 cggtaaacca gctacattta ttaaatttta ctttttattt caagcccagc taagatatgt
     1021 cgatttaaaa actcgatatc taaaaccgct attctgttgt gcttatcaat cttaacaata
     1081 tgatcttgat aatgaattaa tggtccatat ataactttag ctttattatc ttctataaat
     1141 gcttgggaca tttctaagat accttcgcta ttgagtagtt tattaaacat ctcgatttct
     1201 tcatctctta aagctgtaac tccatcatct ttaagctgct taatcaaacc atctctctgt
     1261 tcccctaatt catataacag attatcaaaa tcactttgct tcatttcact tctaacaaaa
     1321 atatatcctg gaaataacat ttttaatgta tttcctttaa tatctcgacg atagtattca
     1381 atctttggaa taaaagcatc aagacctttt cttcttagaa ctgaacacaa cttattttct
     1441 tttgcaacta atgcaaataa tacataccag ttatctttca taacaactcc tatgttttac
     1501 ttgtaacttc caaaggaact ttcattgttt taccaaaaac atcaccaata aaagcaactc
     1561 ttttatgacg atcaattttc ttaatcattt cctctttacc aactaatggt cctttttgaa
     1621 ctattaactt agaatcagta atagttccca ttgaacgggt tattaaatga tcatcattaa
     1681 ataacgattc taaaagttct ttttcattat tggctaatga taaaactttt tgttcatcac
     1741 tttgcataat tctcatcgta gaatccatat ctttttctaa actttcaata atctgataaa
     1801 actcctgtga attaaattca ctttcaataa agatataatt aggaaacatc ggcacttctg
     1861 caaatacctt ctttccatct tttttcatta acttttcaat ttttggtaca aaagcagtca
     1921 tctcaggatg attattaaaa taaaataaaa gatcattgat cttattatca tttgcaaaaa
     1981 taatatacca attcatcata atcctcctta caatataaac taacttttgg tcacaaaact
     2041 gtcttatatt aaagtactct acgaacaaaa aaattctata gttacgtaag cattttatca
     2101 ttatcgaaca aaaaatgata ggataaattg tcaaaaaaca ccgataaaac taaattataa
     2161 atttatttac caaataatta atgttttaaa tagatttaag tactatttct agtcattttt
     2221 ttggtcaaaa gaatctaaaa gaaagaagag tataatatat gccaagaaga ggagaaaata
     2281 tttttagaag aaaagatggc agatgggaag cccggtatgt taaagcaata agtactaata
     2341 ataaaaaact atatggaagt gtttatgcta gtacctatac tgaagttaaa caaaaaagag
     2401 aaaatatatg taattcttta aacaaagaaa aaattaaaaa gtcgaatatt aataatcaag
     2461 tattagaata ttatatagtt gaatggttaa atgtgtatcg aattaaattt aaagccccta
     2521 cttatgtcaa gtattataat gtaatctata attatttatt accatatttt aaagatataa
     2581 aggcgaatga attaaatacc aaacaagttg aattatttac cgcttcttta ttaaaagaag
     2641 gaaaaaatgg taaaccacta tcacctaaaa cagtaaaaga tattctttct ttgtttaaat
     2701 ccattatagg atatatagaa tccgaacata atattagtat aaatactaaa attatatatc
     2761 ctaaaaaaca aaaagaaaaa gaacgcatat tatccaaaga agaatttata aacttgaatc
     2821 aaactttatt acaagaaata gattcatgca aatttggtgt acttatagcc atggctactg
     2881 gattacgaat cggtgagtta tgcgctttaa aatggaaaga tatttcacta aaagaaaact
     2941 atatctatgt taacaaaaca atgcagcgaa taaaaaatat ttacagtgat aatccaaaaa
     3001 caataatcat tgaagattca cctaaaagtt ctacatcaaa tcgaaaaatt ccaattccag
     3061 ctaatcttat tactctattt aaacaattta ggcaaaatga tgattgctat ttattaactg
     3121 gcagaagtga caaatttgta gagccccgat cattagaaag aaaatttcga aaatatatta
     3181 aaaatgcagg aattgaaaaa gctaactttc atatgctaag acatactttt gcaactatgt
     3241 gtatcgaggg tggttttgaa atcaaatgtc ttagtgaaat tcttggtcat agcggttctc
     3301 aaattacctt agaccgctat gttcattcat catttgatct taaaaaaagt tggattgaca
     3361 agttttcaaa tcaaaacatt tcttgtcact aaatatggtc atagtcaaaa agtact
//
LOCUS       DAOMTA010000256         3413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1034_length_3413_cov_2.344550, whole genome shotgun sequence.
ACCESSION   DAOMTA010000256 DAOMTA010000000
VERSION     DAOMTA010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1034_length_3413_cov_2.344550"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLK68_07760"
     CDS             complement(<1..705)
                     /locus_tag="PLK68_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM90343.1"
                     /translation="MQKIFRKYIIIIMSISLASVLLINFFLSTDSLQARTLETFNLKI
                     NQVIQTIENNRTELVSLKSSLDEDYLTRAKAFAYVIEKNPNIIESVTELQNLATLLDV
                     DELHVSDSEGLIAYSSVPKYVGLDFHNGEQMRGFLPILESDDPDEYVIQDAQPNTAEG
                     KIMKYVGVARKDKKGLVQVGLEPTRLLEAQQRNTYSYIFSRFPTNEGEQLFAINKTTN
                     ELIASTNDLNDKEASYY"
     gene            complement(788..1780)
                     /locus_tag="PLK68_07765"
     CDS             complement(788..1780)
                     /locus_tag="PLK68_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM90344.1"
                     /translation="MKLGIIGSGMIVYDLLTFIDIIDEIELTAILGRKESQEKIETLI
                     NKHHIKKAYYDYDELLNDDEIDTIYVALPNHLHYEYTKKALLHNKHVICEKPFTSNVN
                     ELDELIALAKQQNCLLFEAITNQYLPTFKEIKEKISEIGKISIISSNYSQYSSRYNAF
                     KRGEILPAFDVNKSGGALMDLNVYNIHFVVGLFGAPNKVEYFANIEKGIDTSGVAILE
                     YPTFKAICVGAKDCGAPIISTIQGDQGCVKIDGPTSVLTDVQILRNGRDSEILPTGNY
                     HRMYSEFKVFAECIDNNDFTTCNKMLEHSRIVMDVLTKARQSANIIFGCERDTK"
     gene            1880..3214
                     /gene="mnmE"
                     /locus_tag="PLK68_07770"
     CDS             1880..3214
                     /gene="mnmE"
                     /locus_tag="PLK68_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRM90345.1"
                     /translation="MNEDIIVAIATSRLEAAISMIRVSGPDCIAFVQKFFTGKIIEKP
                     SHTINYGYIIDDGKRIDEVLVNIYRGTRTFTGEEMVEINCHGGVYITSRVLEVCIKNG
                     ARMAERGEFSKRAFLNGRIDLSQAEAISDIITAKNSYATDLALKGISGSISGFIEDLK
                     EDLIQIITQIEVNIDYPEYDDVEELTASSLLPRSANLLTKMNKILDDSKNIKLVKEGI
                     KTVIIGRPNVGKSSLLNALLREDKAIVTNIAGTTRDIVEGSISIDGVVLNMIDTAGIR
                     ETDDIIESMGVEKSKELIHQADLVLLVIDGSQSLSSEDMQLLELTEDATRIIVLNKAD
                     QGTKVDLDGIVISAKDNQISTLTEEIKKMFELGKIIDNNDHILTNARQTMLLQRASQA
                     LKQAVEAMEMMIPTDLIVTDLYECWNNLKEILGEKAKEDLLDELFKRFCIGK"
     gene            3216..>3413
                     /locus_tag="PLK68_07775"
     CDS             3216..>3413
                     /locus_tag="PLK68_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90346.1"
                     /translation="MDKNRILSARFLGFSKYLGIVAAISFVVFLIINAFNTGNDILFW
                     ISYVLLMLSIIGAIQCICLYFI"
BASE COUNT         1175 a          459 c          578 g         1201 t
ORIGIN      
        1 atagtaactt gcttctttgt cgtttaaatc attagtgctg gcaatcaatt cgttggttgt
       61 tttattgatt gcaaaaagct gctcaccttc atttgtggga aatctagaaa atatatagga
      121 atatgtattc ctttgttgtg cttctaaaag tcgtgttggt tcaagaccga cttgtactaa
      181 tccttttttg tctttacgag ccactccaac atatttcata attttacctt cagcagtatt
      241 aggctgagca tcttgaataa catattcatc agggtcatcg ctttctaaaa ttggtaaaaa
      301 accacgcatt tgttcaccat tatgaaaatc caaaccaaca tatttaggaa ctgatgagta
      361 agcaattaaa ccctcactat cacttacatg taattcatct acatcaagta gtgtagctaa
      421 attttgaagt tcagtgacac tttcaatgat attaggattt ttttcaataa cataagcaaa
      481 tgcttttgct ctagttaaat aatcttcatc aagactagat ttcaaagata ctaattcggt
      541 acgattattt tcaattgttt gaattacttg attaattttt aaattaaatg tttctaaagt
      601 tcgggcttgt aaagaatcag ttgataagaa aaaatttatt aaaagaacag aggctaatga
      661 aatagacatg attatgataa tgtatttcct aaatatcttc tgcatttagc caccctcttc
      721 caatttgtaa tgtatatata ttattatagc attgttacta tgtcttgata ataaaaaagg
      781 atacagatta ttttgtatcc cgttcgcaac caaaaataat atttgcactt tggcgtgctt
      841 tagttaaaac atccatgacg attcgtgaat gttcaagcat tttattacat gtagtaaagt
      901 cattattatc aatacattcc gcaaaaactt taaattcgct atacattcga tgatagttac
      961 cagttggtag aatctcacta tctcggccat ttcttaaaat ttgcacatca gttagaacac
     1021 tggtaggacc atcaattttt acacaacctt ggtcaccttg aattgtagaa atgattggag
     1081 caccacaatc tttagcacca acacagattg ctttaaatgt tggatattcc aagatagcaa
     1141 ctcctgaagt atcaattcct ttttcaatat tagcaaaata ttctacttta ttaggggcac
     1201 caaataaacc aacaacaaaa tgaatattat atacatttaa gtccattaaa gccccaccag
     1261 atttattgac atcaaatgct ggtaatattt caccacgttt aaaagcgtta taacgtgaag
     1321 aatattggga ataattagat gaaataatac tgattttccc aatctcagat attttttctt
     1381 ttatttcttt aaaagttgga agatactggt ttgtaattgc ttcaaataaa agacaatttt
     1441 gttgtttagc taaagcaatt aattcatcta attcattgac atttgaagta aatggttttt
     1501 cacaaataac atgtttatta tgcaataagg ctttttttgt atattcgtaa tggagatgat
     1561 ttggtaaagc tacataaata gtatctattt catcatcatt taacaattca tcataatcat
     1621 agtatgcttt ttttatatga tgtttattta taagtgtttc aattttttct tgagattctt
     1681 ttctccctaa tattgccgtt agttctattt catcaataat atcaatgaat gttaataaat
     1741 cgtagacgat cattccgcta ccgataattc caagtttcat gttgaattcc tccctgctta
     1801 ccaccattat agcattgatt aggtatatta attaataata atttatgata aaatacaaaa
     1861 gataaaggaa ggtgatattt tgaacgaaga tattattgtt gcaattgcaa caagtcgctt
     1921 agaagctgca atttcaatga ttagagtaag tggtccagat tgtattgcct ttgttcaaaa
     1981 attctttaca ggcaaaataa tagaaaaacc tagtcatacg atcaattatg gatatatcat
     2041 tgacgatgga aaaagaattg atgaagtgtt agttaatatt tatcgaggta caagaacatt
     2101 tacaggtgaa gaaatggtgg aaataaattg tcatggtggt gtttatatta cttcacgagt
     2161 attagaagtg tgtatcaaaa atggtgctag aatggctgag cgtggtgagt ttagtaaaag
     2221 agcatttctt aatggtcgaa ttgatttatc acaggctgag gcaattagtg atattattac
     2281 tgctaaaaat agttatgcaa cagatttagc gttaaaagga attagtggta gtattagcgg
     2341 ttttattgaa gatttgaaag aagatttaat tcaaataatc actcagatcg aagttaatat
     2401 tgattatcct gaatatgatg atgttgagga attgactgct tcttcgttat taccacgaag
     2461 tgccaatcta ttgactaaaa tgaataagat tcttgatgat tctaaaaata ttaaattagt
     2521 taaagaggga attaaaacag ttattattgg acgtcctaat gttggtaaga gtagtttatt
     2581 gaatgctctg cttcgagagg ataaagcaat tgtaacaaat attgcaggaa caacccggga
     2641 tattgttgaa ggaagtatta gcattgatgg agtagttctc aatatgattg atactgcagg
     2701 aatccgagaa actgatgata tcattgaaag tatgggggtt gaaaagtcta aagaactgat
     2761 tcaccaagcg gaccttgttt tattggtaat tgatggttcg cagtcattaa gtagtgaaga
     2821 tatgcagtta ttagaactaa cagaagatgc tactagaatt atcgtgctta ataaagccga
     2881 tcaaggaaca aaagtagatt tagatggtat tgttattagt gctaaagata atcaaattag
     2941 cactttgaca gaagaaatca aaaaaatgtt tgagttaggt aagattattg ataataatga
     3001 tcatattctg acaaatgcac gtcaaacaat gttattacaa cgtgctagtc aggctttaaa
     3061 gcaagctgtt gaagctatgg aaatgatgat accaacggat ttgattgtta ctgatttata
     3121 tgaatgttgg aataatttaa aagaaatatt aggagagaaa gcgaaagaag atttattaga
     3181 tgaattattt aaacgctttt gtataggtaa ataaaatgga caaaaataga attttatcgg
     3241 ctagatttct aggcttttca aagtatttag ggattgtggc agctatatcg tttgtagtat
     3301 ttctaataat taatgctttt aatacaggta atgatatatt attttggatt agctatgttt
     3361 tattgatgtt atcaataatt ggagctattc aatgtatctg tctatatttt att
//
LOCUS       DAOMTA010000257         3412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1035_length_3412_cov_2.180518, whole genome shotgun sequence.
ACCESSION   DAOMTA010000257 DAOMTA010000000
VERSION     DAOMTA010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1035_length_3412_cov_2.180518"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..649
                     /locus_tag="PLK68_07780"
     CDS             257..649
                     /locus_tag="PLK68_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90347.1"
                     /translation="MKEKFYRFMQGRYGIDQLNSFLMIVCVICFIVNMFIGSIVLTFI
                     AYGTWLFVIFRMFSKNIYARNRENDKYLNFFSPLSRWLKLKLMSKQDPSNKYFSCPKC
                     KQMVRVPKGHGTVVVTCPNCQNKFEKRT"
     gene            652..1485
                     /locus_tag="PLK68_07785"
     CDS             652..1485
                     /locus_tag="PLK68_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HRM90348.1"
                     /translation="MFGYVVINKPELKFKEFDVYQSYYCGLCKTLKEKYGSRAQISLN
                     FDLNFISILLSGLYEPETKIGESRCVMHPLSKHTTRYNECVDYAAKMTIVLTYFKCED
                     DWLDERKISKQAYKRLLRKAYQEIKKEYPDKLAHIETCLHCINDYESQGITNLDEISK
                     YFGEVMGEICAYKDDEWHDELYEFGFYLGKFIYFIDAYEDIEQDLKKGTYNPFKELYQ
                     TDQFEDKCKDILELMISEATMAFERLPIIENAAIIRNILYGGVWNKYELVRQKRLEGR
                     K"
     gene            1485..2117
                     /locus_tag="PLK68_07790"
     CDS             1485..2117
                     /locus_tag="PLK68_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRM90349.1"
                     /translation="MNPYQILGISSDATDDEVKKAYRTLSKKYHPDANINNPNQAAYT
                     EKFKEVQTAYKTIMDDRKRGFTNRTYGNTQGAGYNGQSQSSYQYNGNDQAAFNEAAGF
                     INARRFQEALNILEQIKTRNAMWFYYSAVAMNGIGNNITAVEYAQTAAQMEPGNLQYL
                     LFLQQLKGGQRQYQTGQQTYGSPFGSTMQCCYSIMLLNCMMNCCCGGRMC"
     gene            2133..>3412
                     /gene="dnaK"
                     /locus_tag="PLK68_07795"
     CDS             2133..>3412
                     /gene="dnaK"
                     /locus_tag="PLK68_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRM90350.1"
                     /translation="MSKVIGIDLGTTNSCMAYMEDGKGIIIPNAEGNKTTPSVVAFTN
                     DGKRLVGENAKRQAVTNPLNTVASIKREMGTAYRKNINGKDYSPQEISAMILQKLKID
                     AEAYLHEPVTEAVITVPAYFNDAQRQATKDAGRIAGLNVKRIINEPTAAALAYGLENS
                     YGQKIMVFDLGGGTFDVSIIEIGNGVIEVLSTSGDNHLGGDDFNEIIAKYIVDEFRRI
                     EGIDLSHDQVAMQRINAAGEKAKIELSTMITTIVNLPFITNTSSGPKNLEVEISRAKF
                     NELTKGLVDRVVIPMQNALNDARLTPQELNKIILVGGSSRIPAVQDKIKEITGKEASK
                     SLNPDECVAQGASIQGGKLSGDIAATGNFDVLLLDVTPLTLSIETMGGVATPLIERNT
                     TVPTSHSQIFTTSANFQTQVEINVLQGERPLAKD"
BASE COUNT         1219 a          420 c          689 g         1084 t
ORIGIN      
        1 tcaatcggtg gggcaataaa ttattttatt cctttaacct attactgatt atttagaaaa
       61 taaaaaatat ttaaattgtt gatgagccag ggaataagtt aaaaataata tagtattatt
      121 agccatgtta ggatatcctg atatggtttt ttatgctata atagtactat tttatatagt
      181 attaatcaaa agttcattaa tagttaataa taaataaata tacttaggtc gtataaaata
      241 aaattaggga gcaaatatga aagaaaagtt ctatcgcttt atgcaaggaa ggtatggcat
      301 tgatcaatta aatagttttt taatgattgt ctgtgtcatt tgttttatcg taaatatgtt
      361 tattggcagt attgttttaa cttttattgc ttatggaaca tggctatttg taatatttag
      421 aatgtttagt aaaaatattt atgcacgtaa tcgagaaaat gacaaatatt taaatttctt
      481 tagtccactt agtcggtggt taaaactaaa attgatgagt aaacaagatc caagtaacaa
      541 atattttagc tgtcctaaat gtaagcagat ggttagagtg ccaaaaggac atggaactgt
      601 tgtagtaact tgtcccaatt gtcaaaacaa gtttgaaaag agaacataaa gatgtttggc
      661 tacgttgtta tcaataaacc agaattaaaa tttaaagaat ttgatgttta tcagtcatat
      721 tactgcggat tatgcaaaac tcttaaggaa aagtatggta gccgggctca aatctctttg
      781 aattttgatt tgaattttat tagtatctta ttgagtggct tgtatgaacc ggaaactaag
      841 ataggggaat cacgatgtgt tatgcaccct ttaagtaagc atacgactcg ttacaatgaa
      901 tgtgttgatt atgccgctaa aatgacgatt gttttaacat attttaaatg tgaagatgat
      961 tggttagatg agcgtaagat tagtaaacag gcatataaaa ggttgttaag aaaagcatat
     1021 caagaaatta agaaagaata tccagataag ttggcacata tcgaaacttg tttacattgt
     1081 attaatgact atgaaagtca aggaattaca aatcttgatg agatttctaa atattttggt
     1141 gaagtaatgg gtgaaatatg tgcttataaa gatgatgaat ggcatgatga attatatgaa
     1201 tttggatttt atttagggaa gtttatttat tttattgatg cttatgaaga tattgaacaa
     1261 gatttaaaaa agggaacata taatccgttt aaagaattat atcaaacgga tcagtttgag
     1321 gataaatgca aagatatttt agaattaatg atatctgaag caaccatggc ttttgaaaga
     1381 ttgccaatta ttgaaaatgc cgcaattata agaaatatct tgtatggtgg ggtatggaat
     1441 aagtatgaat tagttagaca aaaacgattg gaaggtagga aataatgaac ccgtatcaaa
     1501 tattaggaat atcatctgat gcaactgatg atgaagtaaa aaaagcatat cgtacgttga
     1561 gtaaaaaata tcatccggat gcgaatatta ataatcctaa tcaagctgcg tataccgaaa
     1621 agtttaaaga ggtgcagaca gcttataaaa caatcatgga tgatcgtaag cgtggtttta
     1681 caaatcgaac ttatggcaat acccaaggtg cggggtataa tggacaatca caatctagct
     1741 atcagtataa tggtaatgat caagctgctt ttaatgaggc tgctggattt attaatgctc
     1801 gaagatttca agaagcactt aatattttgg aacaaattaa gactcgtaat gcaatgtggt
     1861 tttattatag tgctgtagca atgaatggaa ttggtaataa cattacagca gttgaatatg
     1921 cgcagacggc tgcccaaatg gaacctggaa acttgcaata tctattattt ttacagcagt
     1981 taaaaggtgg tcaaagacag taccagacag ggcagcagac ttatggttcg ccttttggca
     2041 gtacaatgca gtgctgttat agtattatgt tacttaattg tatgatgaat tgttgttgtg
     2101 gtggccggat gtgctagaag gagatatgat taatgagtaa agtaattgga attgacttag
     2161 gtacaacaaa tagttgtatg gcttacatgg aagatggtaa agggattatt atcccgaatg
     2221 cagagggaaa taaaacaacc cctagtgttg ttgctttcac taatgatgga aaacggttag
     2281 ttggtgaaaa tgctaaacgt caagctgtta ctaatccgtt aaatacagta gcatcaatta
     2341 aacgtgaaat gggaactgca tatcgtaaaa atattaatgg taaagactat agccctcaag
     2401 agatttcagc aatgatttta caaaagctaa aaattgatgc tgaggcttat ttacatgaac
     2461 cagtaacaga ggcagtcatt acagttcctg cttattttaa tgatgcccaa cgtcaagcaa
     2521 ctaaggatgc ggggcgaatt gctggtttaa atgttaaacg gattattaat gagccaacag
     2581 cagcagcctt agcttatggc ttagaaaata gctatggaca aaaaatcatg gtatttgatt
     2641 taggcggtgg aacatttgat gtttctatta tcgaaattgg aaatggagta attgaagttt
     2701 tatcaacatc tggagataat catttaggtg gtgatgattt caatgaaatt atagctaagt
     2761 atattgttga tgaatttaga agaattgaag gcattgattt aagtcatgat caagtagcaa
     2821 tgcaaagaat taacgcagct ggtgaaaaag ctaaaataga attatctaca atgattacga
     2881 ctattgtgaa tcttcctttc attactaata ctagtagtgg accaaaaaat ttagaggttg
     2941 aaattagtcg agctaaattt aatgaattga caaaaggctt agttgatcgg gttgttattc
     3001 caatgcaaaa tgctttaaac gatgcacgtt taacaccaca agaattaaat aaaattattt
     3061 tagttggagg ttcttcaaga attccggctg ttcaagataa gattaaagaa attactggca
     3121 aggaagcaag taaatcatta aatccagatg aatgtgtagc ccaaggagct agtattcagg
     3181 gtggtaagtt gtctggtgat atagcagcta ctggaaattt tgatgttcta ttacttgatg
     3241 ttacaccatt gactttatca attgaaacga tgggtggtgt ggcaacgcca ttgattgagc
     3301 gtaatactac agttccaacg agtcatagcc aaatttttac aacttctgct aacttccaaa
     3361 ctcaggtaga aattaatgtt cttcaagggg aacgtccatt agccaaagat aa
//
LOCUS       DAOMTA010000258         3378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1056_length_3378_cov_2.017755, whole genome shotgun sequence.
ACCESSION   DAOMTA010000258 DAOMTA010000000
VERSION     DAOMTA010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1056_length_3378_cov_2.017755"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>813
                     /locus_tag="PLK68_07800"
     CDS             15..>813
                     /locus_tag="PLK68_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90351.1"
                     /translation="MTDYLENGMSEYDIYDRIVKGLPLFAAKLWGKQSLDLAGAESVA
                     KQLGDAPGTNFGYDVENKDGVILHETFNTLKNAEVKTVDGKQALQLTGKESYVTTDLE
                     TAGLGNDLRVKVKRTSASIDEQILFESDYRSIKAVQKDTGKVGFSRENHDYSFNYILP
                     VNEWVELEFKNRKDVTELYVNGKLVSTIDPAKDKTSNEMADLTGNYKPLHATTMLPLA
                     RIGSETNAFVGYVDDVRVGVNDTYNSTMELDNALVIASKLVDKNPNLQ"
     assembly_gap    814..823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <824..2344
                     /locus_tag="PLK68_07805"
     CDS             <824..2344
                     /locus_tag="PLK68_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM90352.1"
                     /translation="GYFISTIPLLNKFNPTQEEITNALNALNNALKDVEYTKANYSRV
                     EKYLKLIDETALSNYTDDSVAMLQAAINNVKYGLPVSMQDTVDGYELALANALASLKL
                     SNATNVNFIDSSLITGKSPSQDASSSIDKALDGNPGTMFHSPWNDTTTKHILEFNFKN
                     TSSVSGISYLPRQTGSNGILTKYAITAKKAGSNEWTQIATGNLKNDATEKQIIFDQAY
                     DVDGIRIQFLESVGGFASAAEIKFHDGTVVVNTEGLKDAIDAAKAIDTKDYSKVSVDN
                     LNDIIEEAEALLNSENPDANDVEVMIKTLNDAKMRLILKANKTTLKELVDTVSVLTSS
                     DYTADSWLDFENALENANDVLDESEPTQEDIDQAYADLLNTCSDLVNIYTYDSLQDLL
                     NKANGYEAKDYTTESYENLQKAITAGKAATADMSNSELKVLITSLNDAINKLEKNEIK
                     VEDGSQTNLSKPGQVTPSVKGDVATGDKVNVLPFALTSLLSLSSILYFMKRRKENN"
     gene            2382..2543
                     /locus_tag="PLK68_07810"
     CDS             2382..2543
                     /locus_tag="PLK68_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90353.1"
                     /translation="MSFTIAYEIIQHYGISSDEYLAANNFDQNNKHRDETINKLRNQL
                     HNYGERMMQ"
     assembly_gap    2970..2979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2980..3200
                     /locus_tag="PLK68_07815"
     CDS             <2980..3200
                     /locus_tag="PLK68_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90354.1"
                     /translation="LVNKTRYKKFINALHKEIVKQANKSQCYTYEELLNYMHLPEALK
                     ILHPCKLFLLDILNHEFYLKIFRLISTR"
BASE COUNT         1304 a          430 c          542 g         1082 t
ORIGIN      
        1 cagtatggaa tgatatgact gactacttag aaaatggtat gagtgaatat gatatttatg
       61 atcgtattgt aaaaggatta ccattatttg ctgctaaatt atggggtaaa caatctttag
      121 atttagctgg tgctgaaagt gtcgctaaac aattaggaga tgctccagga acaaacttcg
      181 gttatgatgt agaaaataaa gatggtgtta ttttacatga aacatttaat actttaaaaa
      241 atgcagaagt taaaactgtt gatggtaaac aagctttaca attaactggt aaagaaagtt
      301 atgtaacaac tgatttggaa actgctggtt taggtaatga tttacgtgtt aaagtaaaaa
      361 gaacaagtgc ttctattgat gaacaaattt tatttgaatc agattatcga agtatcaaag
      421 ctgtacaaaa ggatacaggt aaagttggtt tctctagaga aaaccatgat tactcattta
      481 attacatctt accagtaaat gaatgggttg aattagaatt caaaaataga aaagatgtta
      541 ctgaattata tgttaatgga aaattagtat caactattga tccagctaaa gataaaacat
      601 ctaatgaaat ggctgattta actggaaact ataaaccatt acatgcaact acaatgttac
      661 cacttgctag aattggtagt gaaacaaatg catttgttgg atatgttgat gatgtacgtg
      721 ttggtgtaaa tgatacttat aattctacta tggaattaga taacgcttta gttattgctt
      781 ctaaattagt agataaaaat ccaaatttac aatnnnnnnn nnnggctatt ttatatctac
      841 gattccttta ttaaataaat ttaatcctac tcaagaagaa atcactaatg ctttaaatgc
      901 cttaaataat gcattaaaag atgtagaata tacaaaagct aattattcaa gagtagaaaa
      961 atacttaaaa ttaattgatg aaactgcatt aagtaactat acagatgata gtgtagctat
     1021 gttacaagct gcaatcaaca atgttaaata tggtttgcct gtatctatgc aagatacagt
     1081 agatggatat gaattagctt tagctaatgc attagcttca ttaaaattat ctaatgcaac
     1141 taatgtaaac ttcattgatt catcattaat tactggtaaa tcaccatctc aagatgcatc
     1201 aagtagtatt gataaagcat tagatggaaa tccaggaact atgttccatt caccatggaa
     1261 tgatacaaca actaaacata tcttagaatt taactttaaa aacacttcaa gtgtaagtgg
     1321 aatttcatat ttaccaagac aaacaggttc taatggtatc ttaactaaat atgcaattac
     1381 tgctaaaaaa gcaggttcta atgaatggac acaaattgca actggaaatc ttaaaaatga
     1441 tgcaactgaa aaacaaatca tctttgatca agcttatgat gttgatggaa taagaattca
     1501 attcttagaa tcagttggtg gatttgcttc agcagctgaa atcaaattcc atgatggtac
     1561 tgttgttgta aatactgaag gattaaaaga tgctattgat gcagcaaaag ctattgatac
     1621 aaaagattat tctaaagtat ctgtagataa cttaaatgat attatcgaag aagctgaagc
     1681 attattaaat agtgaaaatc cagatgcaaa tgatgtagaa gttatgatta aaacgttaaa
     1741 tgatgcaaaa atgcgtttaa ttttaaaagc taacaaaact acattaaaag aattagtaga
     1801 tacagtaagt gtattaactt catctgatta tacagctgat tcatggttag attttgaaaa
     1861 tgcattagaa aatgcaaatg atgtattaga tgaatctgaa ccaactcaag aagacattga
     1921 tcaagcatac gctgatttat taaatacttg tagtgattta gtaaatattt atacatacga
     1981 tagcttacaa gatttattaa ataaagctaa tggatatgaa gctaaagact atacaacaga
     2041 aagttatgaa aacttacaaa aagcaatcac tgctggaaaa gctgcaactg cagatatgtc
     2101 aaacagtgaa ttaaaagttt taattacatc tttaaatgat gcaattaata aattagaaaa
     2161 gaatgaaatt aaagttgaag atggaagtca aactaatcta tcaaaacctg gtcaagtaac
     2221 accatctgta aaaggagatg ttgctacagg agacaaagta aatgtattac catttgcctt
     2281 aacttcatta ctttcattat catcaattct ttacttcatg aaaagaagaa aagaaaacaa
     2341 ttaatctagt cacgaaaagg acctaatggt ccttttcgtt tttgagtttt acaattgcct
     2401 atgaaattat tcaacattat gggataagta gtgatgaata tttagctgca aataattttg
     2461 atcaaaataa taagcatcga gatgaaacaa ttaacaaatt gagaaatcaa ttacataact
     2521 atggggaaag aatgatgcaa taattcacta taaaataaat catggataaa ttctactgcg
     2581 gggtgctgtg aaggtcttta catttgaata aattagaaat tcatatggaa tagtgaaacc
     2641 gagtgataaa gactatattg ctaaatctat ttttactgtt aatgttcaaa ataaaagagc
     2701 ttcaaaatta catacttatc tccagttact tgttgttgca agaaatctat gtgcacatga
     2761 tgaattattt tttaattttg ttcaaggtgt aatcaatata tctataacac catatcacag
     2821 ccattttaaa cttaacaaaa cacaaaatgg gaactaattc aagggcgata agatttattt
     2881 gcattattaa ttgtaattgc ttctacgtat tccaatacag ccaatacata agatttctta
     2941 gtccacgtag tcccgtttgg tgctgttacn nnnnnnnnna tcttgttaat aaaacgagat
     3001 ataaaaaatt tattaatgct ttacataagg aaattgtaaa acaagctaat aaaagtcaat
     3061 gttacacgta tgaggaattg ctcaattata tgcatttgcc tgaagctttg aaaatattgc
     3121 atccttgtaa attatttttg ctagatattc tcaatcatga attttattta aagattttta
     3181 gattgatttc tactcgataa aatgatttta taagtccagg ttgagaaggg tattgactac
     3241 gctcggtacc ctctttatat aggggcatgg attattacag taggatagtt aatatgaata
     3301 aacctgtagc aagcaaatta ataacaagca ataattattt ttctttcttt gtaaaaaatg
     3361 tagacaagct gaaagacg
//
LOCUS       DAOMTA010000259         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1063_length_3356_cov_2.273856, whole genome shotgun sequence.
ACCESSION   DAOMTA010000259 DAOMTA010000000
VERSION     DAOMTA010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1063_length_3356_cov_2.273856"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..389)
                     /locus_tag="PLK68_07820"
     CDS             complement(213..389)
                     /locus_tag="PLK68_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90355.1"
                     /translation="MKKRLGLGLGMFLITILSNLSGVMAYSGNSNLPGSAIIIVFVPV
                     MLGIIYLASKGEVK"
     gene            complement(467..1240)
                     /locus_tag="PLK68_07825"
     CDS             complement(467..1240)
                     /locus_tag="PLK68_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRM90356.1"
                     /translation="METLIGILIPFIGTTAGAACVYFMKNKMNDLVQKVLLGFASGVM
                     IAASVWSLLIPAMDMSSDLGKMAFVPAAVGFLLGIAFLLLLDRNVPHMHLDNEEEGPK
                     SQLKKSTMLVLAVTLHNIPEGMAVGVIFAGLASGSQGVTYAGALALSLGIAIQNFPEG
                     AIISMPLKSSGLSKNKSFIYGMLSGIVEPIGAGLTILMASLVVPILPYLLAFAAGAMV
                     YVVVEELIPEASQGHHSNIATIGFAIGFVVMMMLDVALG"
     gene            complement(1242..1754)
                     /locus_tag="PLK68_07830"
     CDS             complement(1242..1754)
                     /locus_tag="PLK68_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM90357.1"
                     /translation="MTTQEKIINVATQEFLNSGYLGASLRKIAKAAHVTTGAMYGYYK
                     NKEALFNDIVEEVSEEFKNDYLVEGINLETIDYIYQNFEVFKLIICCSKGTRYEEYLD
                     CLVNEKTKQFNEHGFDDKLSHIINHSYLFGVFEIVRHQMTEKQAEDFVSDLQEFYQAG
                     WNKLLERKEI"
     gene            complement(1859..2767)
                     /locus_tag="PLK68_07835"
     CDS             complement(1859..2767)
                     /locus_tag="PLK68_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90358.1"
                     /translation="MKNAHVHNLKNIDVTFLVDCLNVVTGVSGSGKSTLVFAVLAKQH
                     YRYFDDIIAVRQESITTTRRSNIATYTGIYDEIRKLFGSLEATKEMGFTAKHFSFNSQ
                     GGRCENCEGLGTVTSNMLFFKDVEVVCPVCQGKRFIPEILALKYHEHSIDDVLHLSVD
                     EGVGFFSDCPKIIKTLKMLQDVGLGYLELGQVLTTLSGGEKQRLKLATTLLTNINKHN
                     LYLIDEPTIGLHPLDIEHLLLLLDRIVDSGNTVVIVEHNQQIINAADWIVDLGPAGGN
                     DGGYVIAAGTPNDIKGNENSIIGEFL"
     gene            complement(2764..>3356)
                     /locus_tag="PLK68_07840"
     CDS             complement(2764..>3356)
                     /locus_tag="PLK68_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM90359.1"
                     /translation="LSRQITVNKTVITATTKMPLTELLHWLEQLEATVSGPQKEAVQQ
                     YIIDLKTKIQRIINVGLGYLHLDRQTMTLSGGEKQRIKLAAALQSQLTGIIYIFDEPT
                     MGLHPKDTAGIINVLKELRDQENTVIVIEHDLDVIKAADYLIDLGPGAGKHGGQILAL
                     GTYHELQNNPSSITGQYFVNKKKCKEYIEQVIDILR"
BASE COUNT         1129 a          705 c          404 g         1118 t
ORIGIN      
        1 atgaggctgt tgcctcatct ttcatataca tagggaaagt tattcaaata gagcaggtat
       61 aagatatgat tagtctttta atcaaaattt tatagtgtgc ataaaggact tagcattatt
      121 ttataaccaa tccatctata ccatagctaa attatctaat attctcattt aaaattatat
      181 catccctgct ttaattctat gtaaagtttt agttatttaa cctcaccctt actagctaaa
      241 tagataattc ctaacattac cggtacaaaa acaataatta ttgcacttcc cggtaaatta
      301 ctattaccac tataagccat aactcctgac aaattagata aaattgttat taaaaacatt
      361 cccaaaccta accctaatct ctttttcatt tctgtcacct ctttaacctc attttactta
      421 attacagaaa cgaaactata caactgtgct taagattatg taaagattaa cctaaggcga
      481 catctaacat catcatcacg acaaacccaa tagcaaaacc aatagttgca atattactat
      541 gatgcccttg tgaagcctcc ggaataagtt cctcaacaac tacataaacc atggctcctg
      601 ccgcaaatgc taatagatat ggtaaaatcg gtactactaa tgaagccatc aaaatagtta
      661 atcctgcacc aatcggttca acaatacctg atagcatccc atatataaat gatttgttct
      721 ttgataaccc agaacttttt aacggcattg aaataattgc tccttcagga aagttttgaa
      781 ttgcaattcc cagcgataaa gctaaagctc ccgcataagt tactccctga cttccagatg
      841 ccaatcccgc aaaaataact ccaactgcca ttccttctgg gatgttatgc aaagttactg
      901 ctaagactaa cattgttgat tttttcaatt gacttttagg accctcttct tcattatcca
      961 aatgcatatg aggtacatta cgatcaagta aaagcaaaaa agctattcct aatagaaaac
     1021 ctactgctgc aggaacaaac gccatttttc ccaaatcact tgacatatcc atcgccggaa
     1081 tcaatagtga ccaaacagaa gctgcaatca ttactcccga agcaaatcct aataatactt
     1141 tctgaactaa atcgttcatt ttatttttca taaaatagac acacgctgcc cctgccgtcg
     1201 ttccaataaa cggaatcaat attccaatta atgtttccat attatatttc ctttctttct
     1261 aataacttat tccagcccgc ctgataaaat tcttgtaaat cactgacaaa atcctcagct
     1321 tgtttctcag tcatttgatg acggacaatt tcaaaaacac caaataaata tgaatgatta
     1381 ataatatgtg ataatttatc atcaaaacca tgttcattaa attgcttcgt tttttcattt
     1441 actaaacaat ctaaatattc ttcataacga gttcctttac tacagcaaat gattaattta
     1501 aaaacttcaa aattttgata aatgtaatca atcgtctcaa gattaattcc ctcaaccaaa
     1561 taatcatttt taaattcctc agacacttct tcaacaatat cattaaaaag tgcctcttta
     1621 tttttataat atccatacat tgctccagtt gttacatgag ccgctttggc aatttttctt
     1681 agcgatgcac caagataccc actatttaaa aactcctgag tcgcaacatt tataattttt
     1741 tcttgtgtag tcatagtcac ctccaaaata taacactgtt atatttcgac attattctac
     1801 ctcagagcta ttgcataagc aaataatatg cataaaaata agaacttcgt agaagttctt
     1861 acaaaaattc accaattatt gaattctcat taccctttat atcatttggt gtcccagcag
     1921 caattacata accaccatca tttcctcccg ctggccccag atcaacgatc cagtcagcag
     1981 cattgataat ctgctgatta tgttcaacga ttactactgt attaccacta tcaacaatac
     2041 gatcaagcaa aagtagcaaa tgctcaatat ccagaggatg taagccaatt gttggttcat
     2101 caattaaata aagattatgt ttatttatgt ttgttagtaa tgtcgttgcc aattttaaac
     2161 gctgtttttc accacccgac agcgttgtta aaacttgtcc taattctaaa taccctaaac
     2221 caacatcttg cagcattttt aaagttttaa ttattttggg acaatcacta aaaaaaccga
     2281 ccccttcatc aactgataaa tgaagcacat catcaattga atgttcatga tattttaatg
     2341 caaggatttc aggaatgaaa cgttttcctt gacaaactgg acagacaacc tcaacatctt
     2401 taaaaaataa catgttgcta gtaacagttc ctaatccttc acaattttca caacgccctc
     2461 cttggctgtt aaaagaaaaa tgtttagccg taaaacccat ttctttagtt gcttctaaac
     2521 ttccaaaaag ctttctaatt tcatcataaa ttcctgtata tgtagcaata ttagagcgcc
     2581 ttgtcgtcgt aatactttct tgtctgacag caataatatc atcaaaatat cgataatgct
     2641 gtttagctaa tacagcaaac accaaagtac ttttaccaga ccctgataca ccggtaacaa
     2701 catttaaaca atcaactaaa aaagttacat caatattttt caaattatga acatgagcat
     2761 ttttcacctc aaaatatcga ttacttgttc gatatactct ttacatttct ttttattaac
     2821 aaaatattgt ccagttattg aactaggatt attttgcaac tcgtgataag ttcctaatgc
     2881 aagaatttga ccaccatgct ttcctgcacc aggtccaagg tcaattaaat aatctgctgc
     2941 tttaattaca tctaaatcat gttcaattac aatcaccgta ttctcttgat ctcgcaattc
     3001 ctttaaaaca ttaataattc ctgctgtatc tttaggatgt aatcccattg ttggctcatc
     3061 aaaaatataa attatccccg ttaactgact ttgtaaagcc gctgccaatt taattctttg
     3121 tttctcaccg ccagataaag tcattgtttg acgatctaaa tgcaaatatc ctaaaccaac
     3181 attaatgatt ctttgaattt ttgtttttaa atcaattata tattgttgga cagcttcttt
     3241 ttgtggtcct gaaacagtag cttccagctg ctcaagccaa tgtagtaatt ccgtcaaagg
     3301 catttttgtt gttgctgtaa taactgtttt attcactgta atttgacgac ttaatg
//
LOCUS       DAOMTA010000260         3354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1064_length_3354_cov_2.440436, whole genome shotgun sequence.
ACCESSION   DAOMTA010000260 DAOMTA010000000
VERSION     DAOMTA010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_3354_cov_2.440436"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLK68_07845"
     CDS             <1..856
                     /locus_tag="PLK68_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM90360.1"
                     /translation="FHHEREFENKNHIISLHQRNSGRVPMHIFHYIVITYVYSGTMII
                     TVENDTVTLNAGDVIIFDKHVPHSVAPTSANDLGVNIVLNENYFSKKFINHLPNDQLI
                     SKFMIELMNSQTNHNHYLLFYTKKDHLITNCIQNILCEHFEPAVCSDDLIDNFIMVLI
                     THLVRKFQYNTNLTVSMFKNEQLMDDILNYIHSHYNEGSLNKMCHDFGYDPSYTSKLI
                     KQFSGKTFKQLVNEERMKKAAILLQNHELPIYEIAHQIGINNLTSFYRRFQAYYQCTP
                     QQYRDRYD"
     gene            complement(861..1775)
                     /locus_tag="PLK68_07850"
     CDS             complement(861..1775)
                     /locus_tag="PLK68_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM90361.1"
                     /translation="MDITVVIIQLIQLFILIGIGYLLGKTSLFRGMFVQQLTNLVLNL
                     TMPCMILSSVMNSIDAPALPLKDIIIAMFILVIILPVAAFLMIRRIKTNQGLYLFMIM
                     YPNVGFIGFPLMQSIFGSEAILATAIINMGFNLSLFTLGIVAINYGENKLTSFDLKKI
                     FSPGVISSILAVLIYTFKLTFPYVIVEPINSIGMMTTPLAMLVIGATLSVYHLKDIFS
                     DYTVYLFTLLIDLIIPILFYPVILLFIKDSMIRGITLIILAMPVANGAVLFARSNGQD
                     EFLAAKTVFISTMLAIFTIPSLVYMFLL"
     gene            complement(1879..>2826)
                     /locus_tag="PLK68_07855"
     CDS             complement(1879..>2826)
                     /locus_tag="PLK68_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90362.1"
                     /translation="MAIFVVIGMAKSLANYYKVDSKLAIALSFVGILLLTPVIVTVEN
                     VKGLPLDNFSAQGLFVCMIAAALAVEIYRWCVQKGFTIKMPDSVPQNVSGAFAAVIPA
                     FLIILLFNILRMGFAMTDFGSAQTFVFTILQQPLQSLGGTLPATILVLLVEAVIWCFG
                     LHGSSIVSSVMNPIWFAQSAENLAAFEAGLAMPHIVNYQFISFFVKLGGVGATLSLTL
                     LCLFKAKSDQYRALGKLGIGASLFNINEPIIFGFPIVLNPMMMIPFILANVSVGIVTY
                     LAIYLGLVPMINGINLPYTIPAVISVAGKGLYYKLFYCF"
     assembly_gap    2827..2836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2837..3081)
                     /locus_tag="PLK68_07860"
     CDS             complement(<2837..3081)
                     /locus_tag="PLK68_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-beta-mannoside permease IIC protein"
                     /protein_id="HRM90363.1"
                     /translation="MDCFMDKIGDAIAPIAQKMTANRYLSAIKEGFFGSTPILIAGSI
                     FLLFTSLPFNGYTEFMESTFGAGWMDFYYLPYQVSFK"
     gene            complement(3091..>3354)
                     /locus_tag="PLK68_07865"
     CDS             complement(3091..>3354)
                     /locus_tag="PLK68_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90364.1"
                     /translation="NNKGKKFLVKRQLFVLLILTSGGRLVAQLLSLLSGYSLIEISND
                     ITMYLSLISIGMLLLKKETLQDRLFKTTVKDISNKNILIKNHI"
BASE COUNT         1257 a          581 c          443 g         1063 t
ORIGIN      
        1 ttttcatcat gaacgtgaat ttgaaaacaa aaatcatatt atttctctac atcaacgaaa
       61 tagtggacga gtaccaatgc atatttttca ttatatcgtt attacctatg tttactcagg
      121 gactatgatt ataacagtag aaaatgatac tgttacctta aatgctggag atgttattat
      181 ttttgataag catgtccccc atagtgttgc tccaaccagt gcaaacgatc ttggagtcaa
      241 tattgtttta aatgaaaatt atttttctaa aaaatttata aatcatcttc ctaatgacca
      301 attgatttct aaatttatga ttgaattaat gaacagccaa actaatcata atcactattt
      361 acttttctat accaaaaaag atcatctaat tacaaactgt atccaaaata ttctttgtga
      421 acactttgaa cctgctgttt gttctgatga tctgattgat aactttatta tggttttgat
      481 tactcattta gttcgtaaat ttcaatataa tacgaatcta actgtcagta tgtttaaaaa
      541 tgaacaactg atggatgata ttttaaatta tatccacagc cattataatg aggggagcct
      601 taataaaatg tgccatgatt ttggttacga tccctcatat acaagtaaat taatcaaaca
      661 attttcagga aaaactttta aacagctcgt taatgaagaa cggatgaaaa aagccgctat
      721 tttacttcaa aaccatgaac tgcccattta tgaaatagcc caccaaatcg ggatcaataa
      781 cttgacttct ttttatcgta gatttcaagc atattaccag tgtactcctc aacaatatcg
      841 ggatcgttac gactaatctc ttataataaa aacatataaa ctagactagg tattgtgaat
      901 attgctaaca tcgttgatat aaaaactgtc ttagcagcta aaaattcatc ttgaccattg
      961 cttcgagcaa ataatactgc tccgttagct actggcattg ctaaaataat caatgtaatt
     1021 ccacgtatca tcgaatcttt gataaacaat aaaataactg gataaaacaa tatcgggata
     1081 attaaatcaa ttaataatgt aaataagtac actgtataat cactaaatat atcttttaaa
     1141 tgatatactg ataatgttgc cccaataact aacattgcta agggagtagt catcatacca
     1201 atagaattaa tgggttcaac tataacataa gggaaagtaa gcttaaatgt ataaattaat
     1261 acagctaata tagacgatat tacccctggt gaaaatatct tttttaagtc aaatgaagtc
     1321 aacttatttt caccataatt aattgcaacg attcctaatg taaataatga taaattaaaa
     1381 cccatattaa ttatcgctgt agctaaaatt gcttcacttc caaaaattga ttgcattaat
     1441 gggaaaccaa taaagcctac attagggtac atgatcataa acaaatataa accttgatta
     1501 gttttaattc gtctaatcat taaaaatgct gctactggta aaataataac taaaatgaac
     1561 atagcaataa taatatcttt taaaggtaaa gctggggcat ctattgaatt catcactgat
     1621 gataaaatca tacatggcat tgtgagattt aagaccagat tagttaattg ttgtacaaac
     1681 atccctctaa atagtgacgt cttgccaaga agatagccaa tcccaattaa aataaataac
     1741 tgtatcagtt gaatgataac tacagtaata tccattattt attacctcat tagtcaagta
     1801 atgcttcttt tttagcttgt tcttctataa atgcctgctt atcctgagct ttaaaaaatg
     1861 gataatagat caaccctgtc agaagcaata aaacaacttg taataaagcc ccttgccagc
     1921 cactgaaatt acagctggaa tagtatatgg cagattgatt ccattgatca ttggcactaa
     1981 tcctaaataa attgctaaat aagttactat ccccactgat acattcgcta aaataaaggg
     2041 aatcatcatc atcggattta agacgattgg aaacccaaaa ataattggtt cattgatatt
     2101 aaataaagat gcacctattc ctaattttcc caaagctcta tattgatcag atttcgcttt
     2161 aaataagcat aataacgtta aactcaaagt tgcccctact ccacctaatt taacgaaaaa
     2221 agaaataaat tgataattaa caatatgcgg cattgctaga ccagcttcga aagcagcaag
     2281 attttctgct gattgagcaa accaaattgg attcatgaca gatgaaacaa tacttgaacc
     2341 atgtaatcca aaacaccaga tcaccgcttc aactaaaagg actaagatcg tggctggtaa
     2401 agttcctccc aaagattgca atggctgttg caaaattgta aaaacaaatg tttgggcaga
     2461 accaaaatca gtcattgcaa atcccattct taaaatatta aataataaaa ttattaaaaa
     2521 ggcaggaata actgctgcaa aagcacctga tacattttga ggcacagaat ctggcatttt
     2581 aatagtaaat cctttttgta cgcaccaacg ataaatctct actgcaagag ctgctgctat
     2641 catacaaaca aataaacctt gcgcactaaa attgtctaac ggcaatccct ttacattttc
     2701 aacagtaaca atcactggtg tcaataataa aattccaaca aaagataatg caatagctag
     2761 tttactatct actttataat aattagctaa tgatttagcc ataccaataa ctacgaaaat
     2821 agccatnnnn nnnnnnaatt taaaacttac ttgatacggc aaatagtaaa aatccatcca
     2881 cccagcacca aaggtgcttt ccataaactc agtatatcca ttgaaaggta aactagtgaa
     2941 taataagaaa atagaccctg caattaaaat tggagttgat ccaaaaaaac cttccttaat
     3001 tgcacttaaa tatcgatttg ctgtcatttt ttgtgcaatt ggtgcaatcg catcaccaat
     3061 cttatccata aaacaatcca taatatcctc ctaaatatgg tttttaatta atatattttt
     3121 atttgaaata tctttgactg tagtcttaaa taaacgatct tgtaaagttt ctttttttag
     3181 tagcagcatt cctattgaaa taagcgaaag atacattgtg atatcattac taatttcaat
     3241 taaactataa ccactaagca aacttaatag ttgtgcaact aaccttcctc ctgaagttaa
     3301 aataaggagc acaaataatt gacgtttaac taaaaatttt tttcccttat tatt
//
LOCUS       DAOMTA010000261         3348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1069_length_3348_cov_2.778925, whole genome shotgun sequence.
ACCESSION   DAOMTA010000261 DAOMTA010000000
VERSION     DAOMTA010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_3348_cov_2.778925"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLK68_07870"
     CDS             <1..574
                     /locus_tag="PLK68_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90365.1"
                     /translation="DVIMEQLEALGIMSVFETVIPLVVSVLVCICCTTSASLSLEGKS
                     RWIMCSVPVKTITIFKAKILVNLTVILPILWISLILLRFAFPQTTIQTVFLFITPTIY
                     ALFISIIGMLLNVKYPRYDWTSEYYAVKGGAVSVLATVGVGLVLSAVPVYLCIFFRDY
                     AQFIVIAITVFVAVITVIAYHLLTKIRLYI"
     gene            571..864
                     /locus_tag="PLK68_07875"
     CDS             571..864
                     /locus_tag="PLK68_07875"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM90366.1"
                     /translation="MNSMCEHLLDKKFKALADRNRRKILQYLKSGRKCAGDIAKQFSI
                     SAPSVSYHLAILKESHLISAIHSKGFIYYDFNSDEISEVIEWLYKISERSEEK"
     gene            861..1088
                     /locus_tag="PLK68_07880"
     CDS             861..1088
                     /locus_tag="PLK68_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90367.1"
                     /translation="MKENYSLHIAEMLFEQRKLSGKSQEDLAKYCGVSKSSVSKWENG
                     TTRPSICQLPKIANFYKITIQKLLTGKEKYE"
     gene            1081..1434
                     /locus_tag="PLK68_07885"
     CDS             1081..1434
                     /locus_tag="PLK68_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM90368.1"
                     /translation="MNKKGFDKQYKYQLIAEDIKLDIFSNRLKANTPLPSIRAYTEKY
                     GVASNTIVSALHLLRDKGIIYSHRTKGYCVAGNIEERKVILANELIDNLIADMKHIGF
                     SQYDLLQLIRRIMKL"
     gene            1516..1863
                     /locus_tag="PLK68_07890"
     CDS             1516..1863
                     /locus_tag="PLK68_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM90369.1"
                     /translation="MSNTLCTAPVSLCKKCNSLDVDYFTRTVTNENGNIIHLTAKEFD
                     LLYFLSSHKGQVFTKEQIYEKVWGYDNAPDASNLSSFIRKLRLKIEPHPDNPRYIITV
                     WGVGYKFSGEEKP"
     gene            complement(1903..2271)
                     /locus_tag="PLK68_07895"
     CDS             complement(1903..2271)
                     /locus_tag="PLK68_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90370.1"
                     /translation="MELDYKAIGKRIKIARIKKNLTQETVADRIGVTPQHVSNIETGN
                     TSVSLTTLVAIANTLTVSVDELLCDTVLASKPVFEKEAREILEDCNEYEIRVLVDVLK
                     ATKTSMRAVKMFEAKLNEDK"
     gene            2428..2652
                     /locus_tag="PLK68_07900"
     CDS             2428..2652
                     /locus_tag="PLK68_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HRM90371.1"
                     /translation="MMKFSKRDSKKMIKEMARLYGLSVSEVREQIQDKIIAVMDSDDP
                     DQQAEFKRMFGNRTPTPEEFICTASRQLKF"
     gene            2672..3037
                     /locus_tag="PLK68_07905"
     CDS             2672..3037
                     /locus_tag="PLK68_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90372.1"
                     /translation="MVQTEKQKKVFLSLCGQRDYDLLTGKEAMTQADFERITYITTVL
                     GYSSCTQELISEHLELACKEAERTDREFDILQDYPEYFQDENVYEQIDEWIEDFISQA
                     PPAKRDEIRQLIKENTEII"
BASE COUNT         1085 a          571 c          644 g         1048 t
ORIGIN      
        1 agatgtaata atggagcaac tagaagcact ggggattatg agtgtatttg aaacggttat
       61 tccattagtt gtttctgtat tggtctgcat ttgctgtact acttctgcgt cactttctct
      121 tgagggtaaa agcagatgga ttatgtgttc tgttccagta aaaacgataa ccattttcaa
      181 ggcaaagata ttagtaaatt tgacagttat tttgcctatt ttgtggatta gtcttatttt
      241 actaagattt gcgtttccac agactacaat acagactgtg ttcttattca ttacacctac
      301 catatatgca ctgtttattt caattatcgg aatgttgcta aatgtgaaat atccacgata
      361 tgattggacg agtgaatatt atgccgtaaa aggtggggct gtttctgttt tagctacggt
      421 tggtgtcggg cttgtcctaa gtgctgttcc cgtatatctg tgtatttttt tccgtgatta
      481 tgcacagttc atagtaattg ccattaccgt atttgtagct gttataactg ttatagcata
      541 tcacctttta actaaaattc gtttgtatat atgaatagta tgtgtgaaca tttattagat
      601 aagaaattta aagctctggc tgataggaac agacggaaaa ttcttcaata tctaaaaagt
      661 ggtagaaaat gtgcaggaga tattgcaaaa caattttcta tttctgcccc ctctgtttct
      721 tatcatctgg caattttaaa agaaagtcat ttgatttctg ctatccattc aaaaggattt
      781 atttattacg attttaattc agatgagatt agtgaagtaa ttgaatggct ttataaaatt
      841 tctgaaagaa gtgaggaaaa atgaaagaaa attactcatt acatattgca gaaatgcttt
      901 ttgaacaaag gaaactctct ggaaaatcac aagaggattt agcaaagtat tgtggcgttt
      961 caaaatcctc tgtatcaaaa tgggaaaacg gtacaacccg ccctagtata tgtcagcttc
     1021 ctaaaatagc taatttctat aagataacca ttcaaaaatt actgactgga aaagaaaaat
     1081 atgaataaaa aaggttttga taaacaatac aaatatcagt tgattgctga agatattaag
     1141 ctagatatat tttcaaatcg tttaaaagca aatacgccat tacctagcat tagagcatac
     1201 actgaaaaat atggtgttgc ttcaaataca atagttagcg cacttcactt attaagagat
     1261 aaaggtatta tatattcaca ccgtacaaag ggctattgtg tagctgggaa tatcgaagaa
     1321 agaaaagtta tactggcaaa tgaactaatt gacaatctaa ttgcagatat gaagcatatt
     1381 ggatttagtc aatatgacct actccaactg atacgaagaa ttatgaaact ataaagctgg
     1441 tgatactatg ggaaaatatt agttgcagaa tttacagata aagaagaagc tctttttaat
     1501 cagttagtag aatggttgtc aaatacttta tgtactgccc ctgtttctct gtgcaagaaa
     1561 tgtaacagtt tagatgtaga ttattttact cgtactgtta ctaatgaaaa tggaaatata
     1621 atacatctaa ctgctaaaga atttgaccta ttatatttcc tttcgagcca caaaggacag
     1681 gtgtttacca aagaacaaat ttacgaaaag gtatggggat atgacaatgc accagacgca
     1741 agcaaccttt cctcatttat cagaaagtta agactgaaaa tcgaaccaca cccagacaac
     1801 ccacgatata tcatcactgt ctggggtgtg ggatataaat tcagcggaga agaaaaaccg
     1861 tagctgtatt tcacaattca ttgtgatctg cacgctacgg ttttatttgt cctcatttaa
     1921 ctttgcttca aacattttta cggctcgcat ggaagttttg gttgctttca aaacatctac
     1981 cagcacacgg atttcatatt cgttacaatc ttccagtatc tcccttgctt ctttctcaaa
     2041 gacaggcttt gaagctaaaa ctgtatcaca tagtaattcg tcaactgata ccgtcaaggt
     2101 attggcaata gcgaccagag ttgttaaact gactgatgta ttaccagttt ctatattgct
     2161 tacatgctgt ggagtaacac ctattctgtc agcaacagtt tcttgagtga ggttcttttt
     2221 aattctggca atcttaattc tcttgccgat agctttatag tcaagctcca tgtaactgac
     2281 acactccttt caaaaaatct attcctatac attgtaacct ttgcaaaaat gctttataat
     2341 agactgataa aggtatccat tccaacagtt atagttttat tcaattttga tagaatatac
     2401 atcttagaaa agaggtgcat taaagatatg atgaaattca gtaaaaggga cagcaagaaa
     2461 atgattaaag aaatggctcg tctgtacggg ctttctgttt cagaagtccg tgagcagata
     2521 caggataaaa tcatagcggt aatggacagt gacgacccag accagcaggc agagtttaag
     2581 cgtatgtttg gcaaccgtac ccctacccca gaggaattta tctgcactgc cagcagacag
     2641 ctaaaatttt aaagatttag gagtggctat tatggttcaa acggagaagc aaaagaaagt
     2701 gtttctgtcc ctgtgcggac agcgtgacta tgacctgctt acaggcaagg aagctatgac
     2761 acaggctgat tttgagcgta tcacttatat cacaaccgtt cttggctatt cttcctgcac
     2821 gcaggaattg attagtgagc atttagagct ggcttgcaag gaagcggaac gcacagacag
     2881 ggagtttgat attttgcaag actatccaga atactttcaa gatgaaaatg tctatgaaca
     2941 gattgacgaa tggatagagg acttcatcag tcaagcaccg ccagcaaagc gggacgagat
     3001 acgccagctt atcaaagaaa atacagagat tatctgatac acaggttatc gcaaccacgg
     3061 catttctgct gggtgtgata gccttttttc attcctgcta aaaaacaggg atttggacgg
     3121 aattaggacg gatttatctt ttatgcttat cctgttgaaa tttgccttat tctggcaagg
     3181 cggtttcttc acgggaggat tgcgaatggc aggagtgcat aaaagaaaat catcaaaaaa
     3241 attttaatcc atgttaaggg agtttacagc tatttccgtg tagaatttcc tcactatctg
     3301 tcgattatat tcactaaagc tcaacgaggt tatcacaact tcttgggc
//
LOCUS       DAOMTA010000262         3347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1070_length_3347_cov_2.612697, whole genome shotgun sequence.
ACCESSION   DAOMTA010000262 DAOMTA010000000
VERSION     DAOMTA010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_3347_cov_2.612697"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..170)
                     /locus_tag="PLK68_07910"
     tRNA            complement(78..170)
                     /locus_tag="PLK68_07910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Ser,seq:tga)
     gene            complement(183..259)
                     /locus_tag="PLK68_07915"
     tRNA            complement(183..259)
                     /locus_tag="PLK68_07915"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Ile,seq:cat)
     gene            complement(296..372)
                     /locus_tag="PLK68_07920"
     tRNA            complement(296..372)
                     /locus_tag="PLK68_07920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Met,seq:cat)
     gene            complement(410..485)
                     /locus_tag="PLK68_07925"
     tRNA            complement(410..485)
                     /locus_tag="PLK68_07925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(450..452),aa:Ala,seq:tgc)
     gene            complement(501..577)
                     /locus_tag="PLK68_07930"
     tRNA            complement(501..577)
                     /locus_tag="PLK68_07930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(541..543),aa:Pro,seq:tgg)
     gene            complement(601..677)
                     /locus_tag="PLK68_07935"
     tRNA            complement(601..677)
                     /locus_tag="PLK68_07935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:Arg,seq:acg)
     gene            complement(750..838)
                     /locus_tag="PLK68_07940"
     tRNA            complement(750..838)
                     /locus_tag="PLK68_07940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Leu,seq:taa)
     gene            complement(849..923)
                     /locus_tag="PLK68_07945"
     tRNA            complement(849..923)
                     /locus_tag="PLK68_07945"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(888..890),aa:Cys,seq:gca)
     gene            complement(1137..2984)
                     /locus_tag="PLK68_07950"
     CDS             complement(1137..2984)
                     /locus_tag="PLK68_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM90373.1"
                     /translation="MKKLKNFKFNYSSLWIGFALAFLYLPLVIMAIFSFNDSKSLSSW
                     SGFSLRWYQELFVNQQMIDAIVVSVSIAILSTVISTVLGTITAIGVSKSKPLLRKLVL
                     QVNNLPIMNPDIVTGISLMLLFSFIKVEKGYLTMLLAHVAFCTPFVITNVLPKVRQLD
                     VNLADAAMDLGATPFQALTKVIIPQIKPGIISGALLAFTMSFDDFIISYFVSGNGIEN
                     ISIVIYNMSKRTNPSIYALATIILVVVLLFVAIGTLVPKFFPKATNKLLSSKTAKIVI
                     AVCLVLSVVWSISTGVSRKTLRVYNWGEYIDKTVISDFEDKFDCRIIYETFDSNEIMY
                     TKYMSGNSYDVLVPSEYMIERMIKEDLLQPVDKSLIPNLDNINEGILGQSFDLSNNYW
                     VPYFCGNVGILYDKTVVDEKDLEAGWSILRNTKYKNQIYMYDSERDSFMVALKALGYS
                     MNTTKEREINEAYQWLLEQREEMNPVYVGDESIDTMISGVKAMAIMYSGDAAAVMSEN
                     PNMGYYMPKEGTNVWFDGFVISKECKQTKLANEFINYMISDKVSYKNTVEVGYLTANV
                     NAAAQAEKEEFKDISAYGLRTGPNDEVFAYQDNAVKEMYNSRWTKVKAK"
     gene            complement(2962..>3347)
                     /locus_tag="PLK68_07955"
     CDS             complement(2962..>3347)
                     /locus_tag="PLK68_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM90374.1"
                     /translation="ILPIHTALTKMDKSLVEASNDLGANPITTFWKITFRLSLGGVLT
                     GITMVFLPAISSFVIPKLLGGGQYSLIGNFIEQQFINVGDWHFGSAVSLILAVLVIVL
                     MGLINRVEKYAGYQEEAKREKTKKL"
BASE COUNT         1080 a          726 c          504 g         1037 t
ORIGIN      
        1 agcccgacga gctaccagac tgctccatcc cgcgatattt tttgcattat tttatttcgg
       61 ctgaatttta aattaaatgg cgagggatga gggattcgaa cccccgcggg acgttaatcc
      121 cctgtcggtt ttcaagaccg atcccttcag ccggacttgg gtaatccctc gcattcttaa
      181 attggtggac cctgttggac tcgaaccaac gaccgatcgg ttatgagccg atagctctaa
      241 ccaactgagc taagggtcct tgttgtttct tgacttacgg gcaccgttat taaattggta
      301 gcggggaagg gattcgaacc cccgacccac cgggtatgaa ccgattgctc tagccaactg
      361 agctaccccg ccgtaatcaa ctacagcaat gctataattt aataattaat ggtggagcct
      421 agcgggatcg aaccgctgac cccctgcgtg caaggcaggt gctctcccag ctgagctaag
      481 gccccaatta ttattttaaa tggtcgggaa gacaggattt gaacctgcga ccccctggtc
      541 ccaaaccagg tgcactacca agctgtgcta cttcccgttc tatttctttt tttactcaaa
      601 tggcgcgcta ggcaggaatc gaacccacaa cctcttgatc cgtagtcaag cactctatcc
      661 agttgagcta ctagcgcatc gttcacttac aacgcctatc tatattaaca catcttatta
      721 gcttttgcaa gatttattct tttaagaaat ggtgcccagg gccggaatcg aaccggcacg
      781 gattttaagg tccgcaggat tttaagtcct gtgcgtctac cagtttcgcc acctgggcaa
      841 cacattaatg gaggttccaa ccggactcga accgatgata acagagttgc agtctgttgc
      901 cttaccaact tggccatgga accattaact tgttgttctt gtttttgtca gtcggtgtca
      961 tcaccagact gctcatttat tatatgttaa acagaaaata atttcaagta ttttttataa
     1021 aaaaattacc attttcttat atataagaaa aaacacacta aaaaagattc attttatcaa
     1081 taatttttat atttatattt ttaaaaaaac tgctgatttt taatcaacag ttttaactat
     1141 tttgctttca ctttcgtcca gcgagaatta tacatctctt ttacagcatt atcctgataa
     1201 gcaaatactt catcattcgg gccagtgcgc agtccataag cactaatatc tttaaactcc
     1261 tctttttcag cctgagccgc tgcattaaca tttgcagtta aatatcctac ttcgacagta
     1321 ttcttataag atactttatc acttatcata taattaataa attcatttgc aagttttgtt
     1381 tgcttgcact ctttcgaaat aacaaatcca tcaaaccaga cattagtccc ttctttcggc
     1441 atatagtaac ccatattagg attttccgac attactgctg ctgcatcacc agaatacatt
     1501 atcgccatcg ccttaacacc actgatcatt gtatcaattg actcatcacc tacataaacc
     1561 ggattcattt cttcacgttg ctctaacagc cactgataag cttcatttat ttcacgttct
     1621 tttgtagtat tcattgaata tcctaaagct ttgagagcaa ccataaaact atcacgctct
     1681 gaatcataca tataaatttg atttttgtat tttgtgttcc gtaagatact ccatcctgct
     1741 tctaaatctt tttcatctac aacagtttta tcatacaaaa tacctacatt accacaaaaa
     1801 tatggtaccc aataattatt actcaaatcg aatgactgtc ctaaaattcc ttcattaata
     1861 ttatccaaat taggaattaa tgatttatca acaggctgta ataaatcctc tttgatcatt
     1921 cgttcaatca tatattctga aggaactagt acatcataac tatttccgct catatattta
     1981 gtgtacataa tttcatttga atcaaacgtt tcataaataa tccgacaatc gaacttatct
     2041 tcaaaatcac taataactgt tttatcaata tactctcccc aattatacac tcgtaacgtt
     2101 ttacgactga caccagtaga aattgaccac actacagata aaactagaca aacagcaata
     2161 actatcttag cagttttact gcttaatagt ttatttgtcg cttttggaaa gaatttcggc
     2221 actaatgttc caattgccac aaaaagtaat acaacgacta atataatagt cgcaagtgca
     2281 taaatactag gattagttcg ctttgacata ttgtaaataa cgatcgaaat attttcaatt
     2341 ccattcccgc taacaaaata agaaataata aaatcatcaa atgacatagt aaaagccaat
     2401 aaagcccctg aaattattcc cggtttgatc tgagggataa ttacttttgt aagagcctga
     2461 aatggagttg cccccaaatc catcgcagca tcggctaaat taacatctaa ctgtcgtacc
     2521 tttggtaaaa catttgtaat tacaaatgga gtacaaaaag ctacatgagc taataacata
     2581 gttaaatagc ccttttctac tttaataaac gaaaacaaca acatcaagga tatcccggta
     2641 actatatcag ggttcattat tggtaaatta tttacttgta aaactaactt acgtaataat
     2701 ggttttgact ttgatactcc aattgctgtg atcgttccca aaactgttga aataacggtt
     2761 gaaaggattg cgatactaac tgacacaaca atagcatcga tcatttgttg atttacaaat
     2821 aactcttgat accagcgtaa tgaaaaacca ctccagcttg aaagtgattt agaatcattg
     2881 aacgaaaaaa ttgccataat cacaagcggt aaatacaaga acgctaaagc aaaaccaatc
     2941 cataaactac tataattaaa cttaaagttt tttagttttt tcacgtttag cctcctcttg
     3001 ataccccgcg tatttttcaa cccggttaat aagtcccatg aggacaataa ctaaaacagc
     3061 taaaatcaaa gaaacagcac taccaaaatg ccaatcccct acattaataa attgctgttc
     3121 aataaaatta ccaattagag agtactgtcc accccctaac aattttggaa ttacaaatga
     3181 ggaaatagct ggtaaaaata ccattgtaat tcctgttaat actccaccta atgacaatct
     3241 aaaagtaatt ttccaaaaag tagtaatcgg attcgctcct agatcattgc ttgcctcaac
     3301 taaagattta tccattttag ttaaagcggt atgaataggt aaaatca
//
LOCUS       DAOMTA010000263         3340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1079_length_3340_cov_2.191172, whole genome shotgun sequence.
ACCESSION   DAOMTA010000263 DAOMTA010000000
VERSION     DAOMTA010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1079_length_3340_cov_2.191172"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLK68_07960"
     CDS             complement(<1..468)
                     /locus_tag="PLK68_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90375.1"
                     /translation="MDRFTQMLEKTLMPIAEKVEQNRYLSAIREGFTSIMPFLIIGSI
                     FLLLSNLPSEALNNFLGSIFGAENIAKLGYLLEPTFNIMALLVIIGIAKSLLEYHHIK
                     DTSALILPIITFLLLNNFTITLTTEAGETVTSGGVIPMGYLGAAGLFVAMICTI"
     gene            complement(575..1225)
                     /locus_tag="PLK68_07965"
     CDS             complement(575..1225)
                     /locus_tag="PLK68_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM90376.1"
                     /translation="MNETSFELILHSGNSKSSSMEAIEYARQGDIQEADFKMNEAQEE
                     LLLAHKIQSKLLAKSAKVDHFNPNMLLVHAQDHLCGAQTQLEMAKEIISLYEQVQEIK
                     TYLGIENFQKQKNMRVLLVCGQGMSTSLLVQTMYLYADEGDYIESSSFEELVGVIGDY
                     DVVLVSPQIRYRMPVIERMMTLRTQIVGLIDMKAYGKLDGQKIYNQAKELFMKIKH"
     gene            complement(1240..1713)
                     /locus_tag="PLK68_07970"
     CDS             complement(1240..1713)
                     /locus_tag="PLK68_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM90377.1"
                     /translation="MKNSLQGQSEAGELLKYFKEEYFMGMIDAKNQKEVIQLMCEVAN
                     KYNEFEEDLFQSCIRREELGSTAYGQLIALPHPDHLISQKTIVITAILDKPIVWSKQK
                     AQLIFLICVEKGNQKDLRVLFECISKFMMDQQSVQDVICRGDYQTFTKNLGKLIG"
     gene            complement(1717..3126)
                     /locus_tag="PLK68_07975"
     CDS             complement(1717..3126)
                     /locus_tag="PLK68_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HRM90378.1"
                     /translation="MMLNERQKQIFLCLDENKNSFIKGIELANKVNCSLKTLQNTVKE
                     MKIILEKYQLEIISMTSKGYNLIIHDQQEYNDFKQLLLESDDKKDFNNQGYRITYILS
                     TLLSDNHFVKADDLASQMYVSRSSVSSDLKIVKRVLEKYQLKIEHKPNYGMIVVGLEK
                     DKRDCIIKEQLELQLGHSTIKKEWLATISDIVVDSLMKAKYRISDVVLQNLVLHICVS
                     IQRMQHGIYIDNPQMARQYGHEHVIAKKILDELASIYHFIVTENEISFLAIHLLGKRS
                     YEENDLISKEIDYFVNGMLEEIKIKTDIDFYGDVELKISLALHLVPLLVRLESQMQLQ
                     NTMIQDIQANYPLAYDVAVIAASYISEQKHYQLSSDEVGYLAVHFSLSLSKKENKINP
                     KKVLIICNARRGDYLMIQHTFLKEFNEMISDLEIINALEIPQYNLDDYDCIFATFLNH
                     PLIPKRALRINFFIDQKDI"
BASE COUNT         1115 a          621 c          374 g         1230 t
ORIGIN      
        1 aatagtacaa atcattgcaa caaacaaacc tgcagctcca agatatccca taggaataac
       61 tccccctgaa gtgactgttt caccagcttc agttgtcaat gtaattgtaa aattatttaa
      121 caataaaaac gtaataattg gtaaaatcaa tgcacttgta tctttaatat gatgatattc
      181 taacaatgat ttagcaattc caataattac aagcagtgcc ataatattaa aagtcggttc
      241 taacaaataa cccaacttag caatattttc agcaccaaaa atacttccta aaaaattatt
      301 taaagcttcc gaaggtaaat ttgataacaa caagaatatt gaaccaatga ttaaaaaagg
      361 catgatgctc gtgaagccct cacgaattgc acttaaataa cggttttgtt caaccttctc
      421 agcaattggc attagagttt tctctaacat ttgtgtaaat ctatccataa tagtttcctc
      481 cctttcttta caattacatt atattattta taaatgaaag ttaccttaca cataatttcg
      541 ctatacttag agcaattcta tgcaaactct ggctctaatg cttgattttc ataaataatt
      601 cttttgcttg attataaatt ttttggccat ccaacttccc atatgctttc atatcgatta
      661 gtcctactat ttgtgtacgc agcgtcatca ttcgttcaat tacaggcatt cgataacgga
      721 tctgtggact caccaaaaca acatcataat cacctatcac acctactagt tcttcaaatg
      781 atgatgattc aatataatcc ccttcatcag catataaata cattgtttgt actaacaaac
      841 ttgttgacat cccctgtccg catacaagca atactcgcat atttttttgt ttttgaaaat
      901 tttctatacc taaatatgtt tttatttctt gaacttgctc ataaagacta ataatttctt
      961 ttgccatttc taattgtgtt tgtgccccac aaagatgatc ttgagcatga actaaaagca
     1021 tatttggatt aaaatgatct acttttgcac ttttagctaa caattttgat tgaattttat
     1081 gcgctaataa taactcttct tgagcctcgt tcattttaaa atctgcttct tgaatatccc
     1141 cctgacgagc atattcaatt gcttccatcg aagatgattt agaatttcca gaatgtaaaa
     1201 ttaattcaaa acttgtttca ttcatctttt ctctccccct tatccaatca gttttcctaa
     1261 attctttgta aaagtttgat aatcacctct acaaataaca tcttgaacac tttgctgatc
     1321 catcataaat tttgaaatac actcaaataa tactcttaaa tctttttgat ttcctttttc
     1381 aacacaaatc aaaaaaatca attgtgcctt ttgcttcgac catacgatcg gtttatctaa
     1441 aatagcggta ataacaatcg ttttttgaga aatcaaatga tcaggatgcg gtaaagcaat
     1501 cagctgtcca tatgccgtac tgcctaattc ctcgcgacga atacatgatt gaaacaagtc
     1561 ttcttcaaac tcattatatt tatttgcaac ttcacacatc aattgaataa cttctttttg
     1621 atttttggca tcaatcatgc ccataaaata ctcctcttta aaatacttta ataattcacc
     1681 tgcttcactt tgaccttgta aagaattttt tattctttaa atatcttttt gatcaataaa
     1741 aaaatttatt cttaaagctc gtttaggaat cagtggatga ttcaaaaatg ttgcaaaaat
     1801 acagtcataa tcatctaaat tatattgtgg tatctctaac gcattgataa tctctaaatc
     1861 actgatcatt tcattaaact cttttaaaaa agtatgttga atcattaaat aatcgcctct
     1921 acgagcatta caaattatta agactttttt aggattaatt ttattttctt tttttgaaag
     1981 cgataaagaa aaatgcactg ctaaataacc tacctcatca ctgcttagtt gataatgttt
     2041 ttgttcactg atatacgatg ccgcaataac tgccacatca tacgctaaag gataattcgc
     2101 ctgaatatct tgaatcattg tattttgtaa ttgcatttgg ctttctagcc gaactaataa
     2161 gggcaccaga tgtaaagcca atgaaatctt caattcaaca tcaccataaa aatcaatatc
     2221 agttttaatc ttaatttcct ccaacatgcc attaacaaaa taatcgattt ctttgctgat
     2281 taaatcattt tcttcataac tacgctttcc aagtagatga attgctaaaa aagatatttc
     2341 attttcagta acaataaaat gatatattga agctaattca tccaaaattt ttttggcaat
     2401 tacatgctca tgcccatatt gccttgccat ttgtggatta tcaatgtata ttccatgctg
     2461 cattcgttga attgaaacac atatgtgtaa aaccaagttt tgtaagacca catcgctaat
     2521 ccgatatttt gctttcatca gactatcaac aactatatca cttatagtag ctagccattc
     2581 ctttttaatt gttgaatgtc ctaattgcaa ttctaattgc tctttaataa tacaatctct
     2641 tttatccttc tctaagccaa caacaatcat gccataatta ggcttatgtt ctatttttaa
     2701 ttgatatttt tcgagaactc tcttgactat ctttaaatca cttgaaacac tggatcgtga
     2761 aacatacatt tgactagcca aatcatccgc tttaacaaaa tgattatcag ataataatgt
     2821 acttaaaata tatgtaattc gataaccctg attattaaaa tcttttttat catcactttc
     2881 taataataac tgtttaaaat cattatattc ttgttgatca tggataatca gattatatcc
     2941 tttgcttgtc attgaaataa tttctagttg atatttttct aaaattattt tcatctcttt
     3001 tactgtattt tgtaatgttt tcaaactaca atttacttta ttagctaatt caatcccttt
     3061 aataaaacta tttttattct catctaagca taaaaatatt tgtttctgtc gttcatttag
     3121 catcataatc tctccttatc cataataaat atactctatt aataattatt tgtccttgca
     3181 gttttttcgc caagcttaga gtaattttct gtaaggatat tttaaaagaa ttgtctatac
     3241 taaaattata agaagattct taaggagcgt caatcatgaa aagaatattt aaccattcat
     3301 cagtacataa tcaccaagca ccatttgtta aacgactaat
//
LOCUS       DAOMTA010000264         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1080_length_3339_cov_2.307247, whole genome shotgun sequence.
ACCESSION   DAOMTA010000264 DAOMTA010000000
VERSION     DAOMTA010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_3339_cov_2.307247"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1016)
                     /locus_tag="PLK68_07980"
     CDS             complement(6..1016)
                     /locus_tag="PLK68_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM90379.1"
                     /translation="MNISNTKNLRQINRDKVMDIFMEQETCTKNQLALATGLSLATIT
                     NILKELLASNEVLKDGELESTGGRKSVVYSLNSDYSRMLTISVNKEYRRGHLIYRVYN
                     LADEIVFSDEVIKKEVKVNDIYDIINIVLAKFDNIKVIGISMPGIINDGRVTSTGLTN
                     FNDIDLYSLLTNKYSQVIILGNDVNTAVIGFYLAEKNYENVCLLFQPGEGYGGVGTVI
                     NGQLVTGKTNCAGEIQYLPLSQDDQLKLLQSPHGTIELLSKIVICLTAIVNPEIVAIS
                     CTNLERASDLDETLKQMVPAKYLPKIKKVTNLSEYILIGLQLICKDSLKTNLVIKKQS
                     II"
     gene            complement(1020..1637)
                     /locus_tag="PLK68_07985"
     CDS             complement(1020..1637)
                     /locus_tag="PLK68_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90380.1"
                     /translation="MDEFLQGINASIYLKWILMQNNHYSLNIKVDDNDNNTIIISNEI
                     VIGKIIYYGKGIFEEELNDRQTKEKIFYLHFQLTHFNHAVELFKEMINCALEATNRPQ
                     IRVLLCCSGGLTTTLFASKMQELAKLENLSYEIFATGYSRLFEIANDYDIILIAPQVA
                     YMLPQAKRRLPDKEVVTIPTRIFATNDYSGALKIIYDWYQNKEEK"
     gene            complement(2012..2221)
                     /locus_tag="PLK68_07990"
     CDS             complement(2012..2221)
                     /locus_tag="PLK68_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM90381.1"
                     /translation="MQVILDLFTELYEKQDILSKLTQSSSLQGYGYSVIHCLDAIGIL
                     DGPNITKIAEHLKMTRGELVKLSKN"
     gene            complement(2223..2768)
                     /locus_tag="PLK68_07995"
     CDS             complement(2223..2768)
                     /locus_tag="PLK68_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRM90382.1"
                     /translation="MFIDLTLEVTPKLTQDAKGNESKANFGHLGTHFDVMNKEFPLSY
                     LERNGVIFDVRGIDEIEVIDLEKIRSGDFVIFYTGFLEKAGYGTKEYFQAHPQLSNNL
                     IEKLLDKKVSIIGIDCAGIRRGKEHTPMDQYCADHNAFVVENLDNLESLLMQNEFTIN
                     TYPMKFSEMTGLPCRVVAKVD"
     gene            complement(2805..3191)
                     /locus_tag="PLK68_08000"
     CDS             complement(2805..3191)
                     /locus_tag="PLK68_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM90383.1"
                     /translation="MGLIIKKPDDAIIVNKKDGTHVAYYLFDEYEVHLNSLPPQTEQV
                     WHFHQAIEEVILIIEGEIEIHYLDNDKRMRQRVQPNDLILVKDSIHTLINRSTKECKF
                     VVFRLVLDGKNKRDLIKNDKKVVNML"
BASE COUNT         1069 a          625 c          391 g         1254 t
ORIGIN      
        1 gatgcttaaa taatcgattg tttttttatg acaagatttg tttttaaaga atctttacaa
       61 atcagttgta aaccaattaa aatatactca cttaaattag ttactttttt tatcttagga
      121 agatatttag ccggaaccat ctgtttcaat gtttcatcta aatcactagc acgctcaaga
      181 ttagtacaac taatagctac gatttcggga tttacgatcg cggttaaaca aattacaatc
      241 ttactcaaca attcaatcgt tccgtgggga gactgaagca atttcaattg atcatcttga
      301 ctcaagggta aatattgtat ctcaccagca caattagttt tacctgttac aagttgaccg
      361 ttaattactg ttccaacacc accataccct tcacctggtt gaaataacag acagacattt
      421 tcataatttt tttctgctag ataaaaacca attactgctg tatttacatc attacccaat
      481 atgatcactt gactatattt attggttagt aatgaatata aatcaatatc attaaaattt
      541 gttaatccag ttgaagtaac tcgcccatca ttaataatcc ctggcatact tattccaata
      601 acttttatat tatcaaactt agctaaaacg atgttaataa tatcataaat atcattaact
      661 ttaacttcct ttttgattac ttcatcacta aaaactattt catctgccag attataaacc
      721 cgataaatca agtgtccccg ccggtattct ttattaaccg aaatcgttag catccgagaa
      781 taatcgctat tcaaagaata aacaacactc tttcttcctc ccgtcgattc taactcacca
      841 tcttttaata cttcattact tgccagcaat tcctttaaaa tatttgtaat tgtagctaaa
      901 cttagcccag tcgctaaagc taattgattt ttagtacaag tttcttgctc cataaaaata
      961 tccatgactt tatcccgatt tatttgtcgt agatttttag tattactgat attcataggt
     1021 tatttttcct ccttgttttg ataccaatca taaatgattt ttaatgctcc tgaataatca
     1081 tttgttgcaa aaatgcgtgt tggtatcgtt actacctctt tatctggcaa acgtcgctta
     1141 gcttgtggca gcatataagc aacttgcggt gcaattaaaa taatatcata atcattagca
     1201 atctcaaata agcgtgaata tcctgttgca aaaatctcat atgatagatt ttccaatttt
     1261 gccagctctt gcattttact tgcaaataaa gttgttgtca gaccaccgct acagcataat
     1321 aaaactctta tttgcggccg attagttgct tctaatgcac aattaatcat ttctttaaac
     1381 aactccactg catgattaaa atgtgttaat tgaaaatgaa gataaaagat tttttccttt
     1441 gtttgacgat catttaactc ttcctcaaaa attcccttgc cataataaat tattttccca
     1501 attactattt cattactgat gatgatcgta ttattatcat tgtcatcaac tttgatattt
     1561 aaactataat gattgttttg cattaatatc cattttaaat aaatcgatgc attgattccc
     1621 tgcaagaact catccattat gagcacctcc cctaacttat gacaagtatt gtcataagta
     1681 ttataatata ttttgtatcc gtttacaatc aatctattaa gttaataaaa caggcactaa
     1741 ttttgttaat aaaactagcg cctctgaaca atactgctat ttgcttaatt cttcaatttg
     1801 atggtttaaa taattattac agttaaaaat tctttaacaa ctatcatttt ttgctcatct
     1861 tgtcatttta aaaaagttat atcacgttca atatactttt gatgtcgtgc ctgctgctct
     1921 aaaaataaat gttctctggc aactgtaagt ttaaaatata tctctttttt attattatca
     1981 ttttgatatg ttgttattaa attattagaa actaattttt tgataatttt actaattccc
     2041 cacgagtcat ttttaggtgt tctgctattt ttgtaatgtt aggcccatca agaattccaa
     2101 ttgcatcaag acaatgaatt actgaataac cataaccttg caaactgctg ctttgggtta
     2161 gctttgataa tatatcttgt ttttcgtata gttcagtaaa taaatcgaga atcacttgca
     2221 ttttaatcta ccttggcaac tacccgacat ggcaagcctg tcatttcact aaatttcatc
     2281 ggataagtat ttatagtaaa ctcattttgc attaataaac tttctagatt atctaaattt
     2341 tcaacaacaa atgcattatg atcagcacaa tattgatcca tcggcgtatg ttctttaccg
     2401 cgacgaatcc cagcacaatc aattccaata atactaactt tcttatctaa caatttttca
     2461 atcagattat ttgataattg agggtgggct tgaaaatatt ctttagtacc atatcctgcc
     2521 ttttctaaaa atcctgtata aaaaatcaca aaatcaccag atctaatttt ttccaaatca
     2581 atcacttcaa tttcatcaat acctcggaca tcaaaaataa ctccatttct ttctaaataa
     2641 gataatggaa actctttgtt catcacatca aaatgagtgc ctaaatgccc aaaattagct
     2701 ttactttcat ttcctttagc atcctgagtt agttttggag taacttctaa agttaaatca
     2761 ataaacatat tcttcctcct tatattgttt ctttggaaac aattctacag catatttaca
     2821 acctttttgt cattcttgat tagatcacgt ttattcttac catcaagtac taatctaaaa
     2881 acaacaaact tacattcttt cgtactacga ttaatcaatg tatgaatact atctttaact
     2941 aatattaaat catttggctg aactctttgc cgcattctct tatcattatc taaatagtgg
     3001 atttctatct ctccttcaat aattaagatt acttcctcga tagcttgatg aaaatgccat
     3061 acttgttcag tttgcggtgg taaactgttt aagtgaactt catactcgtc aaacaaataa
     3121 taggcaacat gagttccatc ttttttattt acgatgattg catcatcagg ctttttaatt
     3181 attagtccca ttgttttatc atataccttt ctttggtttt aaatcctagt ttttgatata
     3241 gtgcgattgc atcttttgaa gaattcaagc aaatcattcc acatttacct tgtccttttg
     3301 ttaataagtc actaacaatt ctactagcaa taccttgat
//
LOCUS       DAOMTA010000265         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1082_length_3338_cov_2.341456, whole genome shotgun sequence.
ACCESSION   DAOMTA010000265 DAOMTA010000000
VERSION     DAOMTA010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_3338_cov_2.341456"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..>3338)
                     /gene="nifJ"
                     /locus_tag="PLK68_08005"
     CDS             complement(207..>3338)
                     /gene="nifJ"
                     /locus_tag="PLK68_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRM90384.1"
                     /translation="IYACRQVGMPMICSHSVQEVMDLGGIAHLTAIKSSVPVMHFFDG
                     FRTSHEIQKVEVMDYDVLESLLDKEALAKYKKNAMNPHTNPIERGGAENDDIYFQGRE
                     AQNKHYDAVVEVVADYMKKISEITGREYAPFTYYGAPDAARVIVAMGSVTETIKETID
                     EMNRRGDRVGLIKVHLYRPFSAKYLLKVLPNTVEKVAVLDRTKEMGATGEPLYLDVVS
                     VLKDVKHVRAIVGGRYGMGSKDTTPRQIKAVYDHLLEDAPFTSFTIGINDDVTNLSLK
                     EDPEFNVDADYTACLFYGLGSDGTVSANKSSIKIIGDHTDLYSQAYFAYDSKKAGGAT
                     RSNLRFGNTPIRATYYVNNADFISCSLDNYVIKYDMLKNLKDGGTFLLNTEFSKEEII
                     DYLPNRVKKQLADKKAKFYIINANKIAGEIGMGRRTNTILQSAFFALNPQILPIEKAV
                     EYMKEMAKKTYSKKGDAIVQLNYKAIDAGKDAIEEVAVDNSWSDLTVTATRSTTGDEH
                     FDEFVSVINSLDGYDLPVSAFMDKLDGSMKSGVAIKEKRAIATEVPRWNKDNCIQCNN
                     CVMVCPHATIRAFLLDEEEMANLPENIGDDVLVPMGKDMGGLVYRIQVSPDNCVGCGL
                     CVTECPGKKGEKALEMVSVKDELVHAPLADYMYANVKYRDDKYPLTTAKGVGFMRPYF
                     EVSGACGGCGETPYYRLASQLFGKDMMIANATGCSSIYSGSTPSTPCAIDGNGQGPAW
                     ANSLFEDNAEFGFGMKLAENYKVGHLLRIIEENKDACEPELKALLEEYVEINGDRAKE
                     RELVPQIMTAVKASSNEAIKELLNHEGDMVSKSQWIIGGDGWAYDIGYGGLDHVIASN
                     QNVNILVLDTEVYSNTGGQSSKSSQAGSIAKFTAGGKSAAKKDLAQIAMAYGHVYVAQ
                     VAMGANPVQTIKAFKEAEAYNGPSLIIAYSPCMEHGIKGGLANHQRQQKDAVSCGYFN
                     LLRYDPRLEDAGKNPLQVDSKAPDFDKFKDFLLSENRFAQLLKVNPEHAEALMEKCLA
                     DAKKRRLRLDRMA"
BASE COUNT         1049 a          672 c          463 g         1154 t
ORIGIN      
        1 tgtgtttctt aattctgttt agttttcaat gatctccact cttatctcga gtgcatgatt
       61 attctatcac taatatttgc tcttgtaaag aattttatac atttttttta tttttttatt
      121 aagtttccaa aaaacagtgt aataatcaac tatatttgca gtcgaataag aaaaaatcag
      181 aggatttttc ctctgattta aactttttat gccattctat ctaatcttaa acgacgtttt
      241 ttagcatcag ctaaacattt ctccattaat gcctccgcat gttctggatt aacctttaac
      301 aattgtgcaa aacggttttc agataacaag aaatctttaa atttatcaaa gtctggtgct
      361 ttagaatcaa cttgcaaagg attctttcct gcatcttcaa gacgaggatc atatctcaat
      421 aagttgaagt atccacaaga tacagcatct ttttgttgtc tttgatgatt tgcaagacca
      481 cctttaatac catgttccat acatggagag taagcaatga ttaaagaagg tccattataa
      541 gcttcagctt ctttgaatgc tttaattgtt tgtacaggat ttgctcccat agcaacttga
      601 gcaacataaa catgtccata tgccatagcg atttgtgcta aatctttttt agcagctgat
      661 ttacccccag cagtaaattt ggcgattgat ccagcttgag atgatttaga agattgtccg
      721 cctgtattag aatatacttc agtatcaagt actaaaatat taacattttg attacttgca
      781 ataacatgat ctaatccacc atagccgata tcataagccc atccatcacc accaatgatc
      841 cattgagatt tacttaccat atctccttca tgatttaaca attctttaat cgcttcgttg
      901 cttgaagctt taaccgcagt catgatttga ggcacaagct ctctttcttt agcgcgatca
      961 ccattgattt caacatactc ttccaataaa gccttcagtt ccggttcaca agcatcttta
     1021 ttttcttcaa taatacgtaa tagatgccca actttataat tttcagctaa tttcattccg
     1081 aaaccaaatt cagcattatc ttcgaataat gagttagccc atgctggtcc ttgaccattt
     1141 ccatcaatcg cacaaggtgt agaaggagta gaaccactat aaattgaact acatccagta
     1201 gcattagcaa tcatcatatc cttaccaaat aattgtgaag ccaaacgata gtatggtgtt
     1261 tcaccacatc caccacaagc acctgaaact tcaaaatacg gtctcatgaa accaacacct
     1321 ttagctgtag ttaatggata cttatcatca cgatatttaa cattagcata catgtaatca
     1381 gctaatggag catgtactaa ttcatctttc acactaacca tttcaagagc tttttcacct
     1441 ttcttacctg gacattcagt aacacataaa ccacatccaa cacagttatc cggtgaaact
     1501 tggatacgat atactaatcc acccatatct ttacccattg gaactaaaac atcatcacca
     1561 atgttttctg gtaaattagc catttcttct tcatcaagca agaacgctct gatcgttgca
     1621 tgagggcaaa ccattacaca gttattacat tgaatacagt tatctttatt ccaacgagga
     1681 acttcagttg caattgcacg cttttctttg attgccactc ctgatttcat tgaaccatct
     1741 aatttatcca tgaaagcaga aacaggtaaa tcataaccat ctaaactatt gattactgaa
     1801 acaaattcat caaaatgttc atctccagta gtacttctag ttgcagttac tgttaaatca
     1861 gaccaagaat tatcaacagc tacttcttca attgcgtctt tacctgcatc aattgctttg
     1921 tagttaagtt ggacaatagc atcacctttt ttactatatg ttttctttgc catttctttc
     1981 atatattcaa cagctttttc aattggtaag atttggggat ttaatgcaaa gaatgcagat
     2041 tgaagaattg tattagttct tcttcccata ccaatttcgc cagcaatttt attggcatta
     2101 attatataga atttagcctt tttatctgct aattgttttt ttactcgatt tggtaaataa
     2161 tcaataattt cttcttttga gaactctgta tttaacaaga atgttccacc atctttaaga
     2221 ttttttaaca tatcatattt aattacatag ttgtctaatg aacaagaaat aaaatccgca
     2281 ttatttacat aataagtagc tctgattgga gtattcccaa agcgtaaatt agagcgtgtt
     2341 gcccctccgg cttttttaga atcataagca aaataagctt gtgaatataa gtcagtatga
     2401 tccccaatga tttttattga agatttatta gctgaaacag taccatctga acctaaacca
     2461 taaaatagac atgctgtata atcagcatca acattaaatt caggatcctc ttttaatgat
     2521 agattagtta catcatcatt aataccaatt gtaaatgaag taaatggagc atcctctaat
     2581 aaatgatcat atactgcttt gatttgacga ggagtagtat ctttagaacc cataccataa
     2641 cgtccaccaa caattgctct tacatgttta acgtctttaa ggacactaac aacatctaaa
     2701 tataaaggct ctccagtagc acccatttct ttagtacgat ctagaacagc tactttttct
     2761 actgtattag gtaatacttt taataagtat ttggcagaga aaggacgata tagatgtact
     2821 ttaattaaac ctacgcgatc accgcgacgg ttcatttcat caattgtttc tttaattgtt
     2881 tctgttactg atcccattgc aacgattact cttgctgcat caggtgcccc ataatatgta
     2941 aatggagcat attctctacc ggtaatttca gaaatctttt tcatataatc agcaactact
     3001 tctaccactg catcataatg cttgttttga gcttcacgac cttggaaata aatatcatca
     3061 ttttcagccc caccgcgctc aattgggtta gtgtgtggat tcattgcatt tttcttgtat
     3121 ttagcaagag cctctttatc taaaagactt tctaatacat cataatccat tacttctact
     3181 ttttgaattt catgagatgt tctaaatcca tcaaagaagt gcattacagg aacagatgat
     3241 ttaattgccg ttaaatgtgc aattcctcct aaatccatta cttcttgaac tgagtgtgaa
     3301 caaatcattg gcattccaac ttggcgacaa gcataaat
//
LOCUS       DAOMTA010000266         3320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1091_length_3320_cov_2.208270, whole genome shotgun sequence.
ACCESSION   DAOMTA010000266 DAOMTA010000000
VERSION     DAOMTA010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1091_length_3320_cov_2.208270"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1516
                     /locus_tag="PLK68_08010"
     CDS             200..1516
                     /locus_tag="PLK68_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRM90385.1"
                     /translation="MLEKIFKLKEKGTTVKTEVIAGITTFLAMAYILAVNPNMLSETG
                     LSYDGVFLATALSSGIATLIMGLLANYPVALAPGMGVNALFTYTLVFSMGYSPAAALA
                     AVVVSGIIFLLISVTGVRKAIINAIPQQLKLAIGAGIGFFIAFIGLKNAGIIIGSEST
                     FVALGKLTDPVVLLAVFGVLITIILMARKTPAAVFYGLVITAIVGIVAGLCGIEGMPA
                     MPSKIVSIDLDTSGFGIFMNGFGELFSHPDCIVALFSLLFVDFFDTAGTLISVANKTN
                     LVDENGELDNIEQALVADSTGTVIGGILGTSTVTSFVESTAGVEAGGRTGLTACTTAI
                     LFFLSILFAPVLSVVTSAVTAPALVAVGISMATQLGGIDWDDIVFAASGFITVIMMIL
                     TYSISDGIAFGFIVYGITSVIAGRSKEIKPIVWALILIFVVYFALI"
     gene            complement(2010..>2298)
                     /locus_tag="PLK68_08015"
     CDS             complement(2010..>2298)
                     /locus_tag="PLK68_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRM90386.1"
                     /translation="GLASYLIHRSNSKNKETALIIYYFQLLINFAWPIAFFNYQSFLL
                     ALAILITLCILVAILIKLFYQIRPLAAFLLLPYMGWILFALYLNFWIFVNN"
     assembly_gap    2299..2308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2309..2397)
                     /locus_tag="PLK68_08020"
     CDS             complement(<2309..2397)
                     /locus_tag="PLK68_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRM90387.1"
                     /translation="MDVYQTVNLPKCSPPGYLFPIVWTFLYIL"
     gene            2631..>3320
                     /locus_tag="PLK68_08025"
     CDS             2631..>3320
                     /locus_tag="PLK68_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRM90388.1"
                     /translation="MKLVSWNVNGIRACITKGFYDVLKESKADIFCVQETKMQEGQIE
                     LEKHGYYTYMNSAEKKGYSGTLVFSKEKPLNYSYGIGIEEHDHEGRVVTLEYEKFYLV
                     NCYTPNSQNELKRLDYRMHWEDDFLAYLKSLEKSKPVILCGDLNVAHQEIDLKNPKTN
                     RKNAGFSDEERAKMTNLLENGFIDTYRYLYPDQEGVYSWWSYRFNARKNNAGWRIDYF
                     IVSQQLKDALKA"
BASE COUNT         1113 a          396 c          626 g         1175 t
ORIGIN      
        1 gaatttgttt tacagaagaa tcatgagtgg tggcttgaat tttatagtcg agcaacttat
       61 tatcgtttaa aaacaagagc cttggaagaa atattatttt atgtaaatat ttcataattg
      121 tcatatatta gcgctttttc cttgaaaaga ctggtatttt agatataata aaaaggtatt
      181 tttaaaaagg agaaagacaa tgcttgaaaa gatttttaaa ttaaaagaaa agggaacaac
      241 ggtcaaaaca gaggttattg ctggaattac tacgttttta gcaatggctt atattttagc
      301 tgttaacccg aacatgttaa gtgagactgg gctgtcttat gatggcgttt tcttagcaac
      361 ggcactatct tcagggatcg caactttgat catggggtta ttagccaact atccagttgc
      421 tttagcaccg gggatgggag ttaatgcatt atttacatat acccttgttt tttcaatggg
      481 gtatagtcct gcagcagcat tggcagcggt tgtagtatca ggaataattt tcttgttaat
      541 ttcagttact ggggtgcgta aagcaattat taatgcaatt cctcaacaat taaagcttgc
      601 gattggagct ggaattggat tttttatcgc ctttattgga ctaaaaaatg ctggaatcat
      661 tattggaagc gaatcaacat ttgtggcgtt agggaagttg actgatccag ttgtattatt
      721 agcagtattt ggggtcttaa taacaattat cttaatggct cgtaagacac cagcagcagt
      781 attttatgga ttggtgatta ccgcaattgt tggaattgta gctggtttat gtggtatcga
      841 gggaatgccg gcaatgcctt caaaaattgt ttcaattgat ttagatacta gtgggtttgg
      901 tatatttatg aatggttttg gtgagttatt tagccatccg gattgtattg tagcattatt
      961 ttcattgtta tttgttgatt tttttgatac tgcaggaaca ttaatttcag ttgcgaacaa
     1021 aactaattta gttgatgaaa atggtgagtt agataatatt gaacaagcat tagttgctga
     1081 ttctacagga acagttattg gaggaattct aggaacctct actgttactt cttttgttga
     1141 atcaacagct ggggttgaag ctggaggaag aacaggatta actgcttgta ctacagcaat
     1201 attattcttc ttatcaattt tatttgctcc agtacttagt gttgtaacta gtgcggttac
     1261 tgctcctgct ttagtggcag ttggaatttc catggcaact caattaggtg gaattgattg
     1321 ggatgatatc gttttcgctg catctggttt catcacagtg atcatgatga ttttaactta
     1381 ttcaatttca gatgggattg catttggttt tattgtttat ggtattacta gtgtaattgc
     1441 tggtagatct aaagaaatca aaccgattgt ttgggcttta attttaatct tcgtagtata
     1501 ttttgcttta atatagtgga aagatacttg tgaactattt taagtaatag tttgtcaagt
     1561 atcttttatt atttaataaa tatgatttaa tttaaagttt tattaataat atatgtgggt
     1621 tagccccttg ctggctaaga ttatttttca atactttcta gttttgaatt gattcaatca
     1681 gctcggtaat aaagcaattt gagtggcaag agcaatcgtt gcaattaaat ctgtgtttcg
     1741 ttagtataaa aatgtaatca actattttga ttttttacta tctaagtaat cttgtttatt
     1801 aatttaccaa aagtagtaac atttataatt actatatttc ttattttatt aatcatttga
     1861 tattagagaa taatattatt ttttcctaat agctattcaa ttatatcaag tgttcaattt
     1921 gacaaagcaa ggtaattgtc aatggaaact tactttaaaa aaataaactt agtttctcgg
     1981 caactatttc taatataata ttcaagactt taattattta caaaaatcca aaaatttaaa
     2041 tataaggcaa aaagtatcca acccatgtaa ggaagtaata aaaaagctgc taaaggacgg
     2101 atttgataaa ataatttgat aagaatagca actagaatac atagtgtaat caatattgct
     2161 agtgctaata aaaaactttg gtaattaaaa aaagcgatag gccatgcaaa attaatcagt
     2221 agttgaaaat aataaataat aagagcagtt tctttatttt tactattact tcgatgaatt
     2281 agataacttg ctaaaccgnn nnnnnnnnat taaaatataa agaaatgtcc aaacgattgg
     2341 gaatagatat ccaggcggag agcacttagg aaggttaact gtttggtata catccattgc
     2401 attcattgta aaaattgcac ttaaagcacc gattcccaga gagattgata gatttaatat
     2461 aatatatttg tattttttca attttatcac ctatattagt atagacagat tatcgaaaaa
     2521 tattaaaaat cttaagaagt gataaagaat gataaatttt tttctaatat agaaaaaaac
     2581 acttctatag aaaagcgctt tcatgtataa taatagagtg aggtaagaga atgaaattag
     2641 tatcatggaa tgttaatgga attagagcct gtattacaaa agggttctat gatgttttaa
     2701 aagaaagtaa agcagatatt ttttgtgtcc aagaaacaaa gatgcaagaa ggtcaaattg
     2761 aattagaaaa gcatggatat tatacttata tgaatagtgc tgaaaaaaag ggttatagtg
     2821 gaactttagt gttttctaaa gaaaaaccat taaattatag ttatggaatt ggtatcgaag
     2881 aacatgacca tgaaggccgc gttgttacgc ttgagtatga aaaattttat ttagtcaatt
     2941 gttatactcc taatagccaa aatgaattga aacgattgga ctatcggatg cattgggaag
     3001 atgattttct tgcatacttg aagtctttgg agaagagtaa accggttatt ttatgtggtg
     3061 atttaaatgt agcccatcaa gaaattgatt taaagaatcc taaaacgaat cgtaaaaatg
     3121 caggtttttc tgatgaagag agagctaaga tgacaaattt attagaaaat ggttttatag
     3181 atacgtatcg atatttatat cctgatcaag aaggtgttta ttcttggtgg tcatatcgat
     3241 ttaatgcccg taagaataat gctggatggc ggattgatta ttttattgtt tcgcaacaat
     3301 taaaagatgc tttaaaagct
//
LOCUS       DAOMTA010000267         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1101_length_3311_cov_1.852887, whole genome shotgun sequence.
ACCESSION   DAOMTA010000267 DAOMTA010000000
VERSION     DAOMTA010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_3311_cov_1.852887"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLK68_08030"
     CDS             <1..374
                     /locus_tag="PLK68_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRM90389.1"
                     /translation="LIKFKVGCSKGTYIRSLCYDLAKALGYPGHMKDLIRTKSGNFSL
                     ENCFTLEQIENGEYTTVSLEEALNSYQQLVVDDEKIIFHGKKIKSDLNEQVVILNRQG
                     KVLAMYGPDGNGYLKSIRGLW"
     gene            374..1249
                     /locus_tag="PLK68_08035"
     CDS             374..1249
                     /locus_tag="PLK68_08035"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRM90390.1"
                     /translation="MKVIYLNEENMIALEPSCVALGFFDGMHLGHQKLIDELLTFDVH
                     PKSYLLDSSFKYLMSLEDKIEFLEKLNFDYLFVLRFNHQLASKEPRQFIDEFIIKPKI
                     KHVVCGFDFHFGNHGSGDSVYLKNNRNNDYEISIIDKLEYEQHKISSSYLRQVLSNGQ
                     VELASQLLGRQYQVTGKVVHGRENGRKIGFPTINVAAIDYVLPKNGVYGAKVIIDGKE
                     YIGMANLGYNPTFTALKQASLEVNIFDFDQNVYGKQVNVMFIKHIRSEKKFPSINDLI
                     EQLNKDKQQIINEMI"
     gene            1334..>2128
                     /locus_tag="PLK68_08040"
     CDS             1334..>2128
                     /locus_tag="PLK68_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM90391.1"
                     /translation="MMLYGWLIFSSFLWGTNVLVMKYMLENTTTYFLAALKVLLSVIA
                     IFVIMKYKKIPFKWYKGSLGIKVSLLSITINFILTFEGLNLITGSSNAIVNSLAPLVT
                     ILLTWLFYHTKVNRYQLIAMIIACIGFLISLDFNISQISLGHLMMIGGIVLYSYGNLL
                     MQHQCKKEDSLPFTFQYLCLSFFQLALITLFIPSSNHLENISITLWVLFIIFSGIGFA
                     IIQLTYFRAVHEIGSVKTSFLLGLNPVFTYIGSLLLQENFNFNKFMA"
     assembly_gap    2129..2138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2189..3052)
                     /locus_tag="PLK68_08045"
     CDS             complement(2189..3052)
                     /locus_tag="PLK68_08045"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM90392.1"
                     /translation="MKKIAFIGVGVMGKAMVQNLSQAGYPVSIYTRTKNKVEDLISDK
                     IIWHDSVAECIKDQDIVMTMVGFPQDVEEIYFSSKGIIANAKKDCILIDFTTSSPLLA
                     KKIFEESSKHSLASLDAPVSGGDIGAKKGTLSIMVGGDENVFKQIEPVLEVLGSNINY
                     VGSAGNGQHTKMANQIALAGALAGVCEALTYAKAVNLDPQIMLDCISSGAAGSWQMTN
                     NAPRILADDFAPGFYIKHYIKDMKIGKAVNDTNNLDLEVLNTVLQQFEQLQTDGFENL
                     GTQALIKYYQR"
     gene            complement(3049..>3311)
                     /locus_tag="PLK68_08050"
     CDS             complement(3049..>3311)
                     /locus_tag="PLK68_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791766.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRM90393.1"
                     /translation="HHLVKTIPHTKLNKYLGNEFMTNSFHHQAIDDIAPGFVVSAITA
                     DGIIEGIEKDNIIAVQWHPEKNHDKIQAGLIKLYKELLEEEK"
BASE COUNT         1088 a          463 c          544 g         1206 t
ORIGIN      
        1 atttaattaa atttaaagtg ggatgttcga aaggaacata tattcgttct ctatgctatg
       61 atcttgcgaa ggctttaggt tatccgggac atatgaaaga tttaattaga actaaatcag
      121 gaaatttttc gttagaaaat tgttttacac ttgaacagat tgaaaatggt gaatacacaa
      181 cagtaagttt agaagaagct ttaaattcat atcaacaatt agttgtagat gatgaaaaaa
      241 taatttttca tggaaaaaaa atcaaatcag atttaaatga acaggttgtt atcttaaatc
      301 ggcaggggaa agttcttgca atgtatggcc ctgatggaaa tggttattta aaaagtatta
      361 gaggtttatg gtgatgaaag taatatattt gaatgaagaa aatatgattg ctttggaacc
      421 aagttgtgtt gctttgggtt tttttgatgg catgcatcta ggtcatcaaa aattgattga
      481 tgagctatta acatttgacg ttcatcctaa atcatatctt ttagatagtt cttttaaata
      541 tttgatgagt ctcgaagata aaatagaatt tctagaaaaa ttaaattttg actatttatt
      601 tgtattacgt ttcaatcatc aattagctag taaggaacca cgacagttta ttgacgaatt
      661 tattattaaa ccaaaaatca agcatgttgt ttgtggtttt gattttcatt ttggaaatca
      721 tggtagtggc gatagtgttt atctaaaaaa taatagaaat aatgattatg aaatttcaat
      781 tattgataaa ttagaatatg aacagcataa aatctcatct tcatatttac gacaagtttt
      841 aagtaatggt caagttgaat tggctagtca attgttaggt cggcaatatc aagtgactgg
      901 aaaagttgtt catggacgtg aaaacggtcg taaaattggt tttccgacaa ttaatgtggc
      961 agcaatagat tatgtacttc ctaaaaatgg agtgtatggt gctaaagtaa tcatcgatgg
     1021 taaagaatat ataggaatgg caaatttagg ctataatcca acctttactg ctttgaagca
     1081 agcttcatta gaagttaata ttttcgattt tgatcagaat gtatatggaa aacaagtaaa
     1141 tgttatgttt attaaacata ttcgcagtga gaaaaagttt ccttcaataa acgatttaat
     1201 tgaacagtta aataaggata aacagcagat aattaatgaa atgatatgat tagtacatat
     1261 cattttttta tgttgctttc ctatatttat tagttttatt tatgaatatc tggggaataa
     1321 taaatttggt gatatgatgt tatatggatg gttaatcttt tctagttttt tatggggtac
     1381 caacgttctt gtaatgaaat atatgttaga aaacactaca acatattttt tagctgctct
     1441 aaaagtactg ttgtcggtta ttgcaatttt tgtaatcatg aaatataaaa aaataccttt
     1501 taaatggtat aaaggcagtc taggaataaa ggtatcatta ctttcgatta caattaattt
     1561 tatacttacc tttgagggat tgaatttaat taccggaagt tcaaatgcaa ttgttaattc
     1621 cttagcaccg ctagtgacaa tcctattgac atggcttttt tatcatacta aggtcaatcg
     1681 ttaccagttg attgctatga taatcgcatg cattggattt cttatttctc tagattttaa
     1741 tattagtcag atttctcttg gtcatttaat gatgattggt ggaattgtgt tatatagtta
     1801 tggtaattta ttaatgcagc accaatgtaa aaaagaagac agtttgcctt ttacatttca
     1861 atatttatgt ttgagcttct ttcaattagc gttaattact ttatttattc ctagcagcaa
     1921 tcatcttgaa aatatttcaa taactttatg ggtacttttc ataatttttt ctggaattgg
     1981 atttgctata attcaattaa cgtattttcg agctgttcac gaaattggaa gtgttaaaac
     2041 aagtttttta ttaggactaa atccagtgtt tacatatatt ggttcattgt tactacaaga
     2101 aaattttaat tttaataaat ttatggcgnn nnnnnnnnat ggttgtagcc atggttattg
     2161 caaacaaaaa aagatagtta gttcataact atctttgata atatttaata agagcttgag
     2221 tgcctaagtt ctcaaatccg tcggtttgta attgttcaaa ctgttgtaaa acagtattta
     2281 aaacttctaa gtcaaggttg tttgtatcat taacagcttt gcctatcttc atatctttga
     2341 tataatgttt tatgtaaaac cccggtgcaa aatcatcagc aagaattcgt ggggcattat
     2401 tagtcatttg ccatgaacca gcagctccac tcgaaataca gtcaagcatt atttgagggt
     2461 ccaaattgac tgctttagca tatgtaagag cttcacaaac tccagctaaa gctccggcta
     2521 aagcaatttg attggccatt tttgtatgtt gaccgtttcc ggctgaacca acataattaa
     2581 tgttgcttcc taagacttct aacactggtt caatttgttt aaagacattt tcatcaccgc
     2641 caaccataat tgacaatgtc ccttttttgg caccaatatc accgcctgat accggagcat
     2701 caagactggc taaactatgt tttgaagatt cttcaaaaat tttttttgct aacaatggac
     2761 tagacgtagt gaaatcaatg agaatacaat cttttttagc attagcaatt atccctttgg
     2821 aactaaagta gatttcctca acatcttgag gaaatcctac catagtcatg acaatgtctt
     2881 gatctttgat acactcagca actgaatcgt gccatataat tttatcacta ataagatctt
     2941 caactttatt ttttgttcgc gtataaatag atacaggata tcctgcttga ctcaaatttt
     3001 gaaccattgc tttacccatt actccgaccc caataaatgc aatttttttc atttttcttc
     3061 ctccagtaac tctttatata atttaattaa acctgcttga attttatcat gatttttttc
     3121 cggatgccat tgtacagcaa taatattatc tttttcaatt ccttcaataa ttccatcagc
     3181 agtaattgca ctgacaacaa agccaggggc aatatcatca attgcctggt gatgaaatga
     3241 attggtcata aactcatttc ctaaatattt atttagtttt gtatgaggaa tagttttcac
     3301 gagatgatga t
//
LOCUS       DAOMTA010000268         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1102_length_3307_cov_2.208180, whole genome shotgun sequence.
ACCESSION   DAOMTA010000268 DAOMTA010000000
VERSION     DAOMTA010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_3307_cov_2.208180"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2583)
                     /locus_tag="PLK68_08055"
     CDS             complement(<1..2583)
                     /locus_tag="PLK68_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRM90394.1"
                     /translation="MKKILAFFLVVVVLLGSTQLTIYADNESQGQEITTSVTNGESTK
                     NENDSSMITNEKTVNNESTSPEKTADVKNSSITGKVKLIINTQYFKKNISTLSAQLAN
                     IEQTYTGELDSTKENSQIFEFKNIVNGTYTLTIQGDGYETVSKEVVVNNSETTVKYVN
                     TNSVADNIEGNGFGTFAYGDFNKDGKIDSKDKKILSTAIFNNDSSNSLLDIDDNNKID
                     LLDLHTFTYAYSDNGTTQVTRLEPVIETQPLLVNNVSLNNDELQGTLEGKIENIFSET
                     AQEETLKLKPEKDEEISQSNPIQMSLEVKKPVAVTQLTIQGTKENAITEGSVIVVAEN
                     GEEIEAVISTAKSRARMASRQAIVQADGSIVIDLGTQIAVKKVTIKVTGTSSKSLAEI
                     SKVEFLNGMEDHIPAPKLDIPTGITVDELDEELYIKWDALNNVTGYKVKITYNGKTEE
                     HASNVNSFNLKSFNDKDLLNGKTYKISVKAINDADKNSLWESDYSAAVEATPQASKVP
                     DAPDNVKAVGAYRSINVSWKDMKSTDSYNVFYREKGNGEYIEAVSKYEGTSYTITSLK
                     DNTKYEVYVTGNNKKGTGPASLVSEATTVNILPAQLPQYKLLNTSNGEGKLSNHINNV
                     YYGNNGVTEIVNSPLDEVSTTTNSALALVDDNYDSYLQINDWDFGVSYHRNWRLTFEF
                     DDTYEMNYISFAGPVNDSSINNIGISYYDESGKEVDDNGRIYFIAHLAKPIKTNKVRF
                     GVQSSNRTMRISEFNFYYYDSLEEDVNALFTDSFHLTVRDDVTSTTLDDLQTRLNTPD
                     EVSQELHPFKDLIQLELNQARQVVEGTALQNIQEIHNGIAASKQGNLGFGGLNSWQPL
                     GYVRY"
     regulatory      complement(2723..2808)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1050 a          596 c          408 g         1253 t
ORIGIN      
        1 atatctaaca tatcctaacg gttgccaaga gtttaatcct ccaaatccaa ggtttccttg
       61 tttacttgct gcaattccat tatgaatctc ttgaatattt tgtagtgcag ttccctcaac
      121 aacttgtctt gcttgattaa gctctaactg aattaaatct ttaaatggat gtaattcttg
      181 tgatacctcg tcaggtgtat tcaatctagt ttgtaaatca tcaagcgttg tagaagtaac
      241 atcatctctt acagttaaat ggaatgaatc tgtaaataaa gcattgacat cttcttctaa
      301 tgaatcatag taataaaaat taaactctga aatacgcatt gttcgatttg aactttgtac
      361 tccaaatcta actttattag tttttattgg tttcgcaaga tgagcgataa aataaattct
      421 tccgttatca tctacttctt taccactttc atcataatat gaaattccaa tattattaat
      481 actactatca ttaactggtc cagcaaatga aatatagttc atttcatagg tatcatcaaa
      541 ttcaaacgtc aaacgccaat tacggtgata actaactcca aaatcccagt cattgatttg
      601 tagataagaa tcatagttat catctactaa agctaaagcg ctattagttg tcgtactcac
      661 ttcatctaac gggctattta cgatttctgt aactccatta tttccgtagt aaacattatt
      721 gatatgattt gataatttcc cttcaccatt tgaagtatta agcaatttat attgtggtaa
      781 ttgagcaggt aaaatattaa ccgtcgttgc ttcacttact aaagaagctg gccctgtacc
      841 ttttttatta ttaccagtaa catatacttc atatttagta ttatccttta aagaagtgat
      901 tgtatatgag gttccttcgt atttacttac agcctcaata tactcgccat tccccttttc
      961 acgataaaaa acattgtaag aatctgtact cttcatatct ttccaagata cattaatcga
     1021 gcgataagca ccaactgctt ttacattatc aggtgcatca ggaacttttg aagcttgtgg
     1081 tgtagcctca actgcagctg aataatctga ttcccaaagt gaatttttat cagcatcatt
     1141 aatcgcttta acactaattt tatatgtttt accatttaat agatctttat cgttaaatga
     1201 ttttaaatta aatgaattga cattagaggc atgctcctca gtcttaccat tataggttat
     1261 ttttacttta taacctgtaa cgttatttaa tgcatcccac ttaatataga gttcttcatc
     1321 taactcatca actgtaattc ctgtcggaat atctaatttt ggggcaggaa tatgatcttc
     1381 catcccattt aaaaactcta ctttagaaat ttctgctaat gatttagaag atgttcctgt
     1441 tactttaatc gtaacttttt ttactgcaat ttgagttcct aaatcaatta caattgatcc
     1501 atcagcctga acgattgctt gtcgtgaagc catgcgagca cgtgatttag ctgtactgat
     1561 aactgcttct atctcttctc cattctcagc gacaacaatt acactacctt ctgttattgc
     1621 attttcttta gtgccttgaa tcgtcaactg tgtaactgct actggctttt taacttcaag
     1681 actcatttga atcggattac tttgtgaaat ttcttcatct ttttccggct ttaacttaag
     1741 cgtttcttct tgagctgtct cactaaaaat attttctatt tttccctcta aagttccttg
     1801 taactcatca ttatttaatg agacattgtt tactaacaat ggctgagttt caataactgg
     1861 ttccaatctt gtaacttgag tagttccatt atcactataa gcatatgtaa aagtgtgtaa
     1921 atctaataaa tcaattttat tattatcatc aatatctaat aaagaattag aactatcatt
     1981 attaaaaatg gcagtagata atattttttt atctttgcta tcaattttac catccttatt
     2041 aaaatcacca tatgcaaatg taccaaatcc attgccttca atattatcag caactgagtt
     2101 tgtattgaca tactttacag tagtttcact gttattaaca acaacctctt tactaacagt
     2161 ttcataacca tcaccttgaa ttgttaaagt atatgttcca ttaacaatat tcttaaattc
     2221 aaagatttga ctattttctt tagtcgaatc taattctcca gtgtaagttt gttcaatatt
     2281 agctaattgt gctgacaaag tactaatatt ctttttaaaa tactgcgtat taataatcag
     2341 ttttacttta cccgttattg aagaattctt aacatcagct gttttttctg gcgacgttga
     2401 ttcattatta actgtttttt cattagttat cattgaagaa tcgttttcat tttttgttga
     2461 ttcaccattt gtaactgatg tagttatttc ttgcccctga ctttcattat cagcataaat
     2521 agttaactgt gttgacccca gcaaaacaac tacgacaaga aaaaaagcaa gtattttctt
     2581 cattgtaatt ctcctttcct atttgttatt tttgtccatt taatgggctt aactcgcatt
     2641 gcaaatcaat ttttactgtg ctgtttctat tttatgcgca caaaaaaatg cacacataaa
     2701 aatagcgtac acatacacga atgaatgcaa ctggctgcca tatcataatt gatgaaggcc
     2761 ctatagcttt gcgtacttct tttttcaaag agtttgccat atttatgaac gccatgatta
     2821 tacaatgact atgtgataat gtcaaaaagt ttgcttaaaa aatgtattat aaaaaaacaa
     2881 ttttattttc actaaaaaaa cctccaaaac caaaataatc acctttatat catgttattt
     2941 ttattccaac tcctctattt taaaatcata tttttacata tattaaaaat taactcataa
     3001 aaagtgtttt atcctttgag ttattgaact aataataact ttttgaaaat aaatacaatt
     3061 tcccaggtaa taaatttcga tatttatttt atataacaaa aaaatcacta gttaaataag
     3121 ctggtcatct tttcgttata ttatttatta cagttaatca ccgattaaaa gactaatttc
     3181 tttattttcc caataatatt atcaacttaa gaccagatct tttaaacaat ttatcaatta
     3241 gaattgaagc ctcaaaggca gtcccggatg tcctgaattt gccttttcaa cagtatcagc
     3301 gcataat
//
LOCUS       DAOMTA010000269         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1111_length_3295_cov_2.671914, whole genome shotgun sequence.
ACCESSION   DAOMTA010000269 DAOMTA010000000
VERSION     DAOMTA010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1111_length_3295_cov_2.671914"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..894
                     /locus_tag="PLK68_08060"
     CDS             169..894
                     /locus_tag="PLK68_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM90395.1"
                     /translation="MIELQNINKFFGNTHVLKDISLRVKEGEKLVILGPSGSGKSTTI
                     RCINFLEEPTSGKVFINGEEMTKKNKTRLVKDNVAMVFQQFNLYPHKTVLENLTLAPM
                     KLQKVKKWNAEENALHMLRKVGLENKAHAYPEMLSGGQQQRVAIARALCSQSKIILFD
                     EPTSALDPEMVQEVLDVMTNLAKENITMICVTHEMGFAEAVADRVIFMDDGEIIEENT
                     PKEFFEHPKTDRAKDFLSKIIRN"
     gene            917..1825
                     /locus_tag="PLK68_08065"
     CDS             917..1825
                     /locus_tag="PLK68_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM90396.1"
                     /translation="MIEINKLTKLIAVGAVVLTLTGCGNSSSSTSDSVLDSIAESGEM
                     KVGISENKLGFALENTKTGDLEGLEVDLVNQIAKDIAKKKGCDPIKVTFTKVNAKTRT
                     SMLDTGELDLSAASVTITEERAKSWNFTSAYFNDPKGAFPKGVDSFKTGGKKVEVAVV
                     MGTTSKDALIKYMEEEKGMSDWADYVEFKEYTTNDDIIVALDSGATDAYCIDYTQLFA
                     YKGTSFEIFADTVGQSFEPQPLGLITRYKTGGEDAEWTKFLQAEVRKFWEDGSITKWL
                     EKQGYASQDAPSDLDEEFGPYHHYKG"
     gene            1828..2565
                     /locus_tag="PLK68_08070"
     CDS             1828..2565
                     /locus_tag="PLK68_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM90397.1"
                     /translation="MAVLFTGFEDCLSQIPLFLESFVKVSLLCAIIAMIISLVIGTIT
                     GLMGTSKRLLIRGISRVYVEFFQNTPIIIILFFLFAFSPYVFGKAVDTFQIGTLVLGV
                     YTGAYMCEIIRSGIASISKGQEEAAASQGFNYWQTMRYIILPQAIRVVVPPLTTQIVG
                     IIKNTSILQTIAVADLMYQAEVWTSYSGHAGPSYIISAIIYFIACYPLTMLSRYFEKK
                     YSSGYSTNKVKKKITSKIKQIQIEEEV"
     gene            2567..3259
                     /locus_tag="PLK68_08075"
     CDS             2567..3259
                     /locus_tag="PLK68_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM90398.1"
                     /translation="MFDAFKNILTMNNIDYFIRGLLLTLQFALINIFFSFLLGLLLAL
                     ARNYGNKLLRAIVIAYVEIFRNTPLLLWLLFARFALTRAFGLQPYTAVIIMFVCFFSS
                     ILCEQIRSGINSISKGQQEAASSQGFNFYQTLRYIILPQAIKMIVPQLLSSLITIIKD
                     TSYLSTIAIADFMYQARAVMANYVLVNEKVACYIIIVVVYFVINFTLSCLVRYLSSPS
                     RKKRVKTSLVTQ"
BASE COUNT         1165 a          448 c          598 g         1084 t
ORIGIN      
        1 agtttacttt aaaattgtta aaaatagtaa atatattaga atgtaaagaa acaattctag
       61 attaattcta tttatggata aaatattatt aatgtgataa aaaattattg acattaaaaa
      121 gtagaactgt ataataagaa ccataaataa aaagaaaggg tgataaaagt gattgaacta
      181 cagaatatta ataaattttt tggcaatacc catgtactaa aagatatcag tcttcgtgtt
      241 aaagaaggag aaaaattagt tatcttaggt ccatcaggct caggaaagtc gacaacaatt
      301 agatgtatta attttttaga agagccaact tctggtaaag tgttcattaa tggtgaagag
      361 atgacaaaga aaaacaaaac tcgtttagta aaggataatg ttgcaatggt ctttcaacag
      421 tttaatttat atcctcataa aactgttttg gaaaatctta ctttagcacc aatgaaatta
      481 cagaaagtaa agaaatggaa tgctgaagaa aatgctttgc atatgttgcg aaaagttgga
      541 ttggaaaata aagctcatgc gtatccagag atgttatcag ggggacaaca gcagcgagta
      601 gcgatagcta gggctttatg ttcgcaatca aagattattt tatttgatga accaaccagt
      661 gctttagatc cagaaatggt tcaagaggta ttagatgtta tgactaatct agctaaggaa
      721 aatattacca tgatatgtgt cacccatgaa atgggattcg ctgaagcagt tgctgatcgt
      781 gtgattttta tggatgatgg agaaatcatt gaagaaaata caccaaaaga attctttgaa
      841 catcctaaaa cagatcgtgc taaagatttt ttatcaaaga ttattagaaa ctagatatat
      901 aagaggggga ataaaaatga ttgaaataaa taagttaaca aaattaattg cagtaggggc
      961 agttgttctt acattaacag gatgcgggaa tagtagttca tctactagtg attcggtttt
     1021 agattcaatt gcagaatctg gagaaatgaa agttggtata tcagaaaaca aattaggttt
     1081 tgccttagaa aatactaaaa caggggattt agaaggatta gaagttgatt tggttaacca
     1141 aatcgctaaa gatattgcta agaaaaaagg ttgtgatcct atcaaagtta catttactaa
     1201 agtaaatgct aaaacacgta cttcaatgtt agatacagga gaattagatt tatcagcagc
     1261 ttcagtgaca atcactgaag aaagagctaa atcttggaac ttcacaagtg cttatttcaa
     1321 tgatccaaaa ggtgccttcc ctaaaggtgt agattcattt aaaactggag gaaagaaagt
     1381 tgaagttgca gttgtaatgg gtactacttc taaagatgct ttgattaaat acatggaaga
     1441 agaaaaagga atgtctgatt gggctgatta tgtggaattt aaagaatata ctactaatga
     1501 tgatatcatt gtggcattag attcaggtgc tactgatgct tattgtattg attatacaca
     1561 attatttgca tataagggga cttcatttga aatctttgca gatactgttg gtcaaagttt
     1621 tgaaccacag ccattaggtt tgatcactag gtataaaact ggtggcgaag atgcagaatg
     1681 gactaaattc ttacaagcag aagttaggaa attctgggaa gatggttcta tcactaaatg
     1741 gttagaaaaa caaggttacg cttcacaaga tgccccaagt gatttggatg aagaattcgg
     1801 tccatatcat cactataaag gataatcatg gctgtacttt ttacaggatt tgaagactgt
     1861 ctttcacaaa tacctctatt tctagagtcg tttgtgaaag taagtctcct ttgtgcaata
     1921 attgccatga tcatctcatt agttattggg acaatcactg gcttaatggg aacctctaaa
     1981 agacttttga tcaggggcat ttctagagtc tatgtcgagt tcttccaaaa tacaccaatt
     2041 atcattatct tatttttctt atttgctttt tctccatatg tttttggtaa agctgtcgat
     2101 acattccaaa ttggaactct agttctagga gtatatacag gagcgtatat gtgtgaaatc
     2161 attcgttcag gaattgcatc aatttctaaa ggacaagaag aggcagctgc atcacaagga
     2221 tttaattact ggcaaacaat gagatatatt attttaccac aagctattag agtggtggtg
     2281 ccaccgctaa caacgcaaat agtagggatt attaaaaaca cttcaatact acaaacaatt
     2341 gcagtagctg atttaatgta tcaagctgaa gtatggactt cttattcagg acatgccgga
     2401 ccttcatata ttattagtgc aattatatat ttcattgctt gttatcctct tactatgtta
     2461 tctcgctatt ttgaaaaaaa atatagtagt ggttattcta caaataaagt taagaaaaaa
     2521 ataacaagta aaatcaaaca aattcaaatt gaggaggaag tataatatgt ttgatgcttt
     2581 taaaaatatt ttaacaatga ataatattga ttactttatt aggggcttac ttcttacctt
     2641 acaatttgct ttgattaata tcttctttag ttttttgtta ggtttattgc ttgccttggc
     2701 aagaaactat ggtaataaat tattaagagc tattgtaata gcttatgtag aaatatttag
     2761 aaatacacca ttactattat ggttattgtt tgcgagattt gcattaacta gagcgtttgg
     2821 tttacaacca tatactgcag taatcattat gtttgtttgc ttcttctcat caattctttg
     2881 tgaacaaatt cgttccggaa ttaattcgat ttctaaagga caacaagaag ctgctagttc
     2941 acaaggtttc aacttttacc aaacactaag atatattatt ttaccacaag ctattaaaat
     3001 gatcgttcct caactattat catctctaat tacaatcatt aaagatacat catacttatc
     3061 aactatcgca atagctgatt tcatgtatca agctcgtgca gtaatggcta actatgtatt
     3121 ggtaaatgag aaagtagctt gttatataat tattgtcgtt gtttactttg tgattaactt
     3181 tacattatct tgtttagttc gctatctttc tagtcctagt agaaagaaac gcgttaaaac
     3241 aagtttagta actcagtaaa agaagataaa ggggatgatt tttatcctct tttat
//
LOCUS       DAOMTA010000270         3294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1112_length_3294_cov_2.240198, whole genome shotgun sequence.
ACCESSION   DAOMTA010000270 DAOMTA010000000
VERSION     DAOMTA010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1112_length_3294_cov_2.240198"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLK68_08080"
     CDS             <1..407
                     /locus_tag="PLK68_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM90399.1"
                     /translation="ITETNKLLDLEVLQPHNIQDTLHAYQLAKRCNEKRVMAEAIKWG
                     QKGARINAISPGIIVTPLAIDEFNGPRGGFYKNMFAKCPAGRPGTADEVANVAELLMG
                     PQGAFITGADFLIDGGATAAYFYGPLKPKNKS"
     gene            423..1391
                     /locus_tag="PLK68_08085"
     CDS             423..1391
                     /locus_tag="PLK68_08085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM90400.1"
                     /translation="MEYVNLGKTGLKVSKICLGCMGFGELDRGGIPGKTAEKEAREII
                     KYALEHGINFFDTANYYSLGASEEILGQALKDYANREDVVIATKLYYPMFDGVNAKGL
                     SRKAVFTEVENSLKRLQTDYIDLYIIHRWDYTTPIEETMEALNDLVRMGKIRYIGASA
                     MYAWQFVKANSIAEKNGWAKFISMQNMYNLVYREEEKEMIPMCQYENIALTPFSPLAH
                     GIFNKAVDTPRTDGCGKLQNDYQLAHDREIIKRVYELAKKYQKPTSQIALAWVLSKPY
                     ITSPLVGSSKIKYLEDALQALDIVLTPDEIAYLEEPYVPHNQYGFR"
     gene            1526..1978
                     /locus_tag="PLK68_08090"
     CDS             1526..1978
                     /locus_tag="PLK68_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRM90401.1"
                     /translation="MKKIVVISSSPRKNGNSEILADQFIKGATSVGHKVIKINLANYR
                     IAPCLACEYCRGHHNRCVLKDDANKVIEEIVNADVFVMATPVYFYSLSAQLKILIDRM
                     FAREYEIRNSPKRKTAYLIVNKCTEQLKALEDLLRCSKRWMKEELFMV"
     gene            2083..2562
                     /locus_tag="PLK68_08095"
     CDS             2083..2562
                     /locus_tag="PLK68_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRM90402.1"
                     /translation="MENYYNYFVNKGNHPNKLDILKEEHKYIFEYFNLQTAALIMDKI
                     IDEAKYYNRPFAARIVYNNKIIRQCFVNSYNEKSIKWLGRKERVCLETKHSSYFIFLD
                     NIDTHNYNQMIFDEEYGLCGGSFPIFIKGVPAGAITVTGLRPHEDHQVIVKALEKLF"
     gene            complement(2715..2888)
                     /locus_tag="PLK68_08100"
     CDS             complement(2715..2888)
                     /locus_tag="PLK68_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90403.1"
                     /translation="MTVAGHIIKVTPNRAIHNIGLNSCISEDNSDSLNIFAKTAESII
                     PINIKLRIGFGKK"
BASE COUNT         1199 a          406 c          624 g         1065 t
ORIGIN      
        1 ctattacaga aacgaataaa ttattggact tagaagtact gcagccacat aatattcaag
       61 atacacttca tgcgtatcag cttgctaaac gctgtaatga aaaaagagta atggctgagg
      121 caattaaatg gggacaaaag ggtgctcgga taaatgccat ctcacctgga attattgtaa
      181 ctccattggc tattgatgaa tttaatggtc caagaggagg tttttataaa aatatgtttg
      241 caaagtgtcc agctgggcgg cctggaacgg ctgatgaagt tgcaaatgta gcagaactat
      301 tgatgggacc ccaaggagca tttattacag gtgcagattt tttaattgac ggtggggcta
      361 ctgctgcata tttttatgga ccactaaaac caaaaaataa aagttaattt aaggagaaaa
      421 gtatggaata tgtaaattta ggaaaaacag gattaaaagt ttctaaaata tgtttaggat
      481 gtatgggatt tggtgaatta gatcgtgggg gaatccctgg aaaaactgca gaaaaagaag
      541 ctcgagaaat tattaaatat gcgctagaac atggtattaa cttttttgac acagctaatt
      601 attatagtct tggtgcgagc gaagaaattc ttggacaagc attaaaagat tatgctaatc
      661 gagaagatgt tgtaattgca actaaattgt attacccaat gtttgatggt gttaatgcaa
      721 aaggattatc acgtaaagct gtttttacag aagttgaaaa ttcgctaaag agattacaga
      781 cggattatat cgatctgtat attattcatc gctgggatta tactactccg atcgaagaaa
      841 caatggaagc gttgaatgat ttagtaagaa tggggaaaat tcgttatatt ggagctagtg
      901 cgatgtatgc ttggcagttt gtgaaagcta atagtattgc agaaaagaat ggatgggcaa
      961 aatttattag tatgcagaat atgtataatt tagtgtatcg tgaagaagaa aaagaaatga
     1021 ttccaatgtg tcaatacgaa aatattgctc ttacaccatt ttcaccactt gctcacggta
     1081 tctttaataa ggcggttgat actccgcgga cagatggctg tggtaaactg caaaatgatt
     1141 atcaattagc tcatgatcgt gaaatcatta aaagagttta tgaattagct aaaaaatatc
     1201 aaaaaccaac tagtcaaatt gctttagcat gggttttaag taagccatat attacatcgc
     1261 cgttagtagg tagctctaaa attaaatatt tagaagatgc tttacaagca ctagatatcg
     1321 tcctgacccc agatgagata gcatatttgg aagaaccata tgtgccacac aatcaatatg
     1381 gttttagata attaatagaa ataaaagatt atgaacttat ccataatctt ttattttaga
     1441 cttaataaac cccttgactt aaagtaaagt tgaagggcta tgataataaa aagaaaatta
     1501 ataaaaaatg aaagaggaag cctatatgaa aaagatagtc gttatatcat caagtcctag
     1561 aaaaaatggt aatagtgaaa ttttagctga tcaatttatc aaaggtgcga cgagcgtggg
     1621 acacaaagta ataaaaatca atttagcaaa ttatagaatt gctccatgcc tagcttgtga
     1681 atactgtcga ggacatcaca ataggtgtgt tttaaaagat gatgcgaata aggtaattga
     1741 ggaaattgtt aatgctgatg tatttgtaat ggcaacacca gtttattttt atagtttgag
     1801 tgcacagctt aaaattttaa ttgatcgtat gtttgcacgt gaatatgaaa tcagaaatag
     1861 ccctaaacga aaaacagctt atttgatcgt gaacaaatgc acggaacaat tgaaagcttt
     1921 agaggattta ttaaggtgct caaaaaggtg gatgaaggag gaattattta tggtttagga
     1981 gcatttaata aaggtgatgc ttacacacac ccaagttatg atgaagctta tcaaatgggt
     2041 tcaacaatat aattaattag atttcttatc aggaggaata aaatggaaaa ttattataac
     2101 tattttgtaa ataaaggaaa tcatccaaat aaactagata ttttaaaaga agaacataaa
     2161 tatatttttg aatattttaa tctccagact gctgctttga ttatggataa aataattgat
     2221 gaagctaaat attataatcg accatttgct gcaagaatag tttataacaa taaaataatc
     2281 aggcaatgtt ttgttaatag ttataatgaa aaaagtatta aatggttagg ccgtaaagag
     2341 cgggtatgtc ttgaaacaaa acattcctcg tattttattt ttcttgataa tatagatacc
     2401 cataattata atcaaatgat ttttgatgaa gaatatggtc tttgtggggg aagttttcct
     2461 atatttataa aaggagtacc agcaggggca attactgtta ctggattaag acctcatgaa
     2521 gatcaccaag tcattgtaaa agcattggaa aagttatttt aatcagtcat tcattagggt
     2581 caattatgat atttttaaga attaagaata agatggatat agtttttgat gccaattgat
     2641 ctaaaaaata gataatgttt atttaaggtg aaaacaatta ttatagctac agttttagga
     2701 atgatcatta atgactattt tttaccaaaa ccaattctta attttatatt gattggaatg
     2761 atactttcag ctgtttttgc aaatatgttt agtgagtctg aattatcttc actaatgcat
     2821 gagtttaatc caatattatg aattgcacga ttaggggtaa ctttgattat gtgtcctgct
     2881 acagtcatta aatatcttgg ttttacacta ttaccacatt cttgagtatc tttagtattt
     2941 actggaattg ctgtttcaac tccagctact cagattattc aaagaacgat tgctactgcc
     3001 gcagtgatta atgaattgat tggtgtaata tttgcaaaaa aaaggttttg aatgggccgg
     3061 agaattaaca tataaaaagg tttaggcata ttctaagaat acgcctttga aattttttga
     3121 atcagttaat gctttgctat taagagttta aaatagttag atattctttc atgtgtcttt
     3181 aaaaaatata attgggaaat acgtttcatt aagaatagaa taaaggttgt tcttaataat
     3241 atcaagaata atttttaaat cattatcagc taaatttatg ttatgattag tata
//
LOCUS       DAOMTA010000271         3282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1122_length_3282_cov_2.043694, whole genome shotgun sequence.
ACCESSION   DAOMTA010000271 DAOMTA010000000
VERSION     DAOMTA010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_3282_cov_2.043694"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLK68_08105"
     CDS             complement(<1..299)
                     /locus_tag="PLK68_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRM90404.1"
                     /translation="MNKQFKLIIKILLMIISGRYYYSILTNEYCYKFVDQKKIQNLKT
                     NTLGIGTYNIKSLNYGKNDLKSFNKDIIALKLDIICLQEVDKNSLRSGNFDMLKI"
     assembly_gap    578..587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<588..1662)
                     /locus_tag="PLK68_08110"
     CDS             complement(<588..1662)
                     /locus_tag="PLK68_08110"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRM90405.1"
                     /translation="MIEEQLKQAKLACRKMQNIDKDTKIKALEAISKNLISNIDYIVA
                     ENKKDVACAKENGISEAMVDRLLLTRSRIESIANDVLKVAGLHDCIGEVVREIKRPNG
                     LIIKQVRIPIGTVATIYESRPNVTVDIAAICIKTNNVCILKGGKEAINSNIALVRVIK
                     EAITNILPENVVNLIEKTDRSVVTEVITANNYIDVVVPRGGAGLIQHVVNNATVPVIE
                     TGAGICHLYIDQEADLEMAVEVAVNAKISRPSVCNAIETILVHQGVANEFLTLLKPRF
                     DKIKIFGDEIVLKYLEGNKATTKNYATEYDDYICNIKVVNDINEAIEHIYDYSTKHSE
                     SIITDNEDTARYFMDSLDSACVYH"
     gene            complement(1662..>2237)
                     /locus_tag="PLK68_08115"
     CDS             complement(1662..>2237)
                     /locus_tag="PLK68_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRM90406.1"
                     /translation="NARLIRNVDGITKEIESMAKDASSKVGTGGMITKIRAAKVCNDF
                     GCDMAIVNGNQPNVLIDLIEGKDVGTYFDGKPGRLLNSRQHWIMYRSMPKGTIVVDEG
                     AKKALVTCHSSLLPKGIIEVRGNFLISQIIDIVDGNDNLLARGMVNYSSDEIRLIKGL
                     NTSEIEDVLHYKDYDEVVHANNLVLVEGVKN"
     assembly_gap    2238..2247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2248..2817)
                     /gene="proB"
                     /locus_tag="PLK68_08120"
     CDS             complement(<2248..2817)
                     /gene="proB"
                     /locus_tag="PLK68_08120"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRM90407.1"
                     /translation="MRDLSNIKNLIIKIGSSSLCDDEGNINKEKILNLIQQIAYIKRK
                     GISITLVSSGAINAGVHVMNLECRPQTIPQKQALAAIGQASLMQIYEDLFSLFNLKCA
                     QILLNHDDFDDRKRLMNFNNAMQALIEYDVVPIINENDTLAVEEIKVGDNDTLASLVV
                     PAVNADMVVLVSDIDGLYDKNPKEYSDAVL"
     assembly_gap    2960..2969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1085 a          618 c          340 g         1209 t
ORIGIN      
        1 atgattttta gcatgtcaaa attgcctgaa cgtaaagaat tcttatctac ctcttgcaaa
       61 caaataatat caagttttaa agcaataata tccttattaa aactttttaa atcatttttc
      121 ccataattta aggattttat attatatgtt cctatcccca aggtattagt ctttaaattt
      181 tgaatctttt tttgatcaac aaatttataa caatactcat tagttaagat gctataataa
      241 taacgacctg atattatcat caataatatc ttaattatta atttaaactg tttattcatt
      301 tatttacctc aattatttac taatttatta ttggataaat tagtaatttt aatacaatca
      361 aaatatttat ctaactttca aaattctaat ctgattcaac taaaaataaa aaagatatag
      421 acagttaatt tgcctatatc tatcttattt gcccattccc aaatatttta tattttgttg
      481 atgtcatttc ctgcagtcca attgggccac gtgcatgtaa tttttgagta ctaattccaa
      541 cttcagcgcc aaatccaaat tctccaccat cagtaaannn nnnnnnntat gataaacaca
      601 ggcagaatct aaagaatcca taaaatatct agctgtatct tcattatcag taataatact
      661 ttcagaatgt ttggttgaat aatcatagat atgctcaatc gcttcattga tatcattcac
      721 aactttaata ttacaaatat aatcatcata ttcagtagca taattcttgg ttgttgcctt
      781 attgccttct agatatttta aaactatttc atcaccaaat atcttaattt tatcaaacct
      841 tggttttaat aacgttaaaa attcatttgc aacaccttga tgaactaaaa tagtttcaat
      901 cgcattacaa actgaaggac gagaaatttt agcattaact gcaacttcaa cagccatttc
      961 taaatcagct tcttgatcaa tgtatagatg acaaattcct gcaccagttt caattactgg
     1021 gacagttgca ttatttacaa catgttgaat caaccctgct cccccccgag gaacgacaac
     1081 atctatataa ttattggctg tgataacttc agtaacaaca ctacgatctg ttttctcaat
     1141 caaattaaca acattttcag gtaaaatatt tgtgattgcc tctttgatta cccttactag
     1201 agcaatatta gaattaatcg cttcttttcc gccttttaga atacaaacat tatttgtctt
     1261 tatacaaata gcagctatgt caactgtaac attagggcga gattcataaa tagtagctac
     1321 tgttccaatg ggaatacgaa cttgtttaat gattaagcca tttggacgtt ttatctcacg
     1381 aacaacttca ccaatgcaat catgtaatcc agctactttt aaaacatcat tagcaatact
     1441 ttcaatacgt gacctagtta ataaaagtcg atcaaccatt gcctcactga ttccgttttc
     1501 cttggcgcaa gcaacatcct tcttattttc ggccacgata taatcaatat tagaaatcaa
     1561 atttttacta atagcttcta aagcttttat tttagtatct ttatcaatat tttgcatctt
     1621 tcgacaagct agtttagcct gttttaattg ttcttctatc attaattttt cactccttct
     1681 actaatacca ggttatttgc gtgaactact tcatcataat ctttataatg taaaacatcc
     1741 tcgatttcac tagtgttcaa tcctttaatc aatcttatct catcacttga gtaatttacc
     1801 attccacgtg ctaacagatt atcattacca tcaacaatat caataatctg cgaaattaaa
     1861 aaatttcctc ttacctcaat aattccctta ggcaacaaac ttgagtggca tgtaactaat
     1921 gcttttttag caccttcatc aacaacgatt gttccttttg gcatacttcg atacataatc
     1981 caatgttgtc tagaattaag gagtcttcca ggtttaccat caaaataagt acccacatct
     2041 tttccttcaa tcagatcaat caaaacgttt ggttggttac cattaacaat agccatatca
     2101 cagccaaagt cattacaaac tttagctgct ctaatcttag taatcattcc tccagttcca
     2161 accttgcttg aagcatcttt tgccatgctt tcaatttctt tagtaatccc atcgacatta
     2221 cgaatcaatc ttgcattnnn nnnnnnntaa aactgcatca ctatattctt ttggattttt
     2281 atcatataat ccatcaatat cactgactag tacaaccata tctgcattaa cagccggtac
     2341 taccagtgat gctaatgtat cattatcacc aactttaatt tcttcgaccg ctaaagtatc
     2401 attttcatta ataattggaa ctacgtcata ttcaatcaat gcctgcattg cattgttaaa
     2461 attcatcaac cgcttacgat catcaaaatc atcatgattt aataaaattt gtgcacattt
     2521 taaattaaat aagctaaata aatcttcata tatttgcatt aatgaagcct gtccaatagc
     2581 tgccagagct tgcttttgag gaatagtttg cgggcgacat tcaagattca ttacatgaac
     2641 tccagcatta atagcaccac tgctgactaa tgtaatactt atccctttac gcttaatata
     2701 tgctatttgt tgaattaaat ttaaaatttt ctctttatta atattccctt catcatcaca
     2761 taatgatgat gaaccaattt taataattag atttttaata ttgcttaaat ctcgcattct
     2821 aacttccccc tgtaattatt ttcttaatta tacaaggttt atctcatctt ctcaacaaaa
     2881 aagtgtttaa gcataaatta ctcaaacact ttatctatcg atttcaactt cataaatata
     2941 tttaaattca ccattgaccn nnnnnnnnna aatttattac aagtatactc gatttcaaaa
     3001 acgtgaatgc acttgtttgc taatatagct gtttcaccag ttagagcaat ttttaaagtt
     3061 ccatttttat tctatgtaac aattttttca taatcaatat tttgaccaac taaaattaga
     3121 tattttttta ttatttattc aatcattatc aatatgtgca ttttctcaaa aataaaaatc
     3181 agtatatact aaaacttttt tagtattgtt attgtgattc tttagccact tttaatagat
     3241 tatcactctt aacctctaaa tcataatcaa aattataatt at
//
LOCUS       DAOMTA010000272         3280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1124_length_3280_cov_2.125581, whole genome shotgun sequence.
ACCESSION   DAOMTA010000272 DAOMTA010000000
VERSION     DAOMTA010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_3280_cov_2.125581"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLK68_08125"
     CDS             complement(<1..451)
                     /locus_tag="PLK68_08125"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536405.1"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRM90408.1"
                     /translation="MASKDMDKLVSHAKTSGFVYQGSEIYDGLANTWDYGPLGVEMKN
                     NIKQLWWKRFIQESPYNVGLDSAIFMNPRVWEASGHVGGFSDPLIDCKECKTRHRADK
                     LIEAYDPSVHSEGWSSEETVKYIKDHNIVCPNCGKSNFTDVRQFKLMF"
     gene            complement(610..1524)
                     /locus_tag="PLK68_08130"
     CDS             complement(610..1524)
                     /locus_tag="PLK68_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM90409.1"
                     /translation="MKYSEKFTSATATHVNFDIYLPTVMIRYKAIIQIHHAMGEHSGR
                     YERFAEYLAHDGFVVVVSDFPGHGTSLYNYEQGYFGIGDATKTLVEDMHRLRNIMASR
                     YPDLPYFMIGNQLGSLVLRQYMAQYGDFIQGAILMGTCGKPHFALIGKLIIKGDAMLK
                     GHMHRSKTVRKNVINQLISRTKNATYVTGDARELEQYQQDPFTDFTYTNNAYEEVFGL
                     IKKVSTIQNIKKIPEYLSVLIISGAKDPFGKFGVGPKWLYEALSHQGVKDITLSLYDR
                     SHHDLLHDQQRLEVYKDVLDWLNQRTFV"
     gene            complement(1527..>3280)
                     /locus_tag="PLK68_08135"
     CDS             complement(1527..>3280)
                     /locus_tag="PLK68_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRM90410.1"
                     /translation="DHPSFGAAIICRTLESHGFKVCFLAQPDWHNVEEFRQFGKPRLG
                     FLISSGNIDSMVNHYTVAKKRRHKDLYTPGSEGFKRPDRAVIVYSQMARQAYKDANII
                     IGGIEASLRRLGHYDYWDNKVRKSIIIDANADLLLYGMGENSIIEVAEALDSGLEIKY
                     LTYLDGTVFKTKNLDDAHEPVMLPSYEEICNDKKAYAKSFHIQYLNTDHFNAKTLVEP
                     YQGWYVVQNRPNRPLKQREFDRAYSYAYNRDSHPSYKQHVPAIDEVKFSLISNRGCFG
                     SCNFCALAFHQGRIVQARSHESIISEASKMVWDPEFKGYIHDVGGPTANFRGPACSKQ
                     NKHGACPTKQCLWPKACNNLEVSHQDYLELLRKLRVLPNVKKVFVRSGIRYDYLMYDK
                     DDTFFKELVQNHISGQLKVAPEHISDQVLDKMGKPRRGLYEKFVDKYYQLNEEYNKKQ
                     FLVPYLMSSHPGCTLDDAIELAEYLRDIHHQPEQVQDFYPTPGTLSTTMYYTGLDPRD
                     MSEVYVPKTAKEKAMQRALIQYRNPKNYDLVYEALVQANRKDLIGFGKNCLIRPRRNN
                     NQRSYQAKNNYQKKRRN"
BASE COUNT         1061 a          667 c          457 g         1095 t
ORIGIN      
        1 cgaacattaa tttgaattgt cttacatcag tgaagttgct tttcccacaa ttaggacaaa
       61 caatattgtg atccttgata tattttactg tctcttcact agaccatcct tcactatgaa
      121 cactcggatc ataagcttcg attaatttat ccgcacgatg acgggtctta cattccttac
      181 aatcaatcaa agggtcagag aatccaccaa catgtccact cgcttcccat acccgcggat
      241 tcataaaaat agctgaatct aatcctacat tatatggtga ttcttgaata aaacgtttcc
      301 accacaattg tttaatattg tttttcattt caacacctaa aggaccataa tcccatgtgt
      361 ttgctaagcc atcatagatt tcagaaccct gatatacaaa tcctgaagtt tttgcatgac
      421 ttactaattt atccatatct ttgcttgcca taatttactc cttatattca tgttatttta
      481 gatatataaa caatgaatga cttgaacaca gtcaagtcaa ataaaggcaa taatttgttt
      541 aatatctatt ttttcgtgca catataaaat acccttatag tagttatttg tcaaccaact
      601 ataagggtat caaacaaagg ttcgctgatt caaccaatca agtacatctt tatacacttc
      661 aagacgctgt tgatcgtgta ataaatcatg atgactacga tcatagagcg ataaagtaat
      721 atctttaact ccttgatgac taagcgcttc atataaccat ttaggcccta cgccaaattt
      781 gccaaaagga tccttagcac cagaaattat caaaactgat aaatattcag gtattttttt
      841 tatgttttgg atagtagaaa ctttttttat taagccaaac acttcttcat aagcgttatt
      901 agtatatgta aaatcagtaa acggatcttg ctggtactgt tctaactctc gagcatcacc
      961 agtaacataa gttgcatttt tagtacgaga aattaattga ttgataacat ttttacgaac
     1021 cgttttggaa cgatgcatat gtcctttaag cattgcatcc cccttaataa ttagttttcc
     1081 aatcaatgca aaatgaggtt tgccacaggt tcccattaat atagcacctt ggataaagtc
     1141 cccatattgt gccatatatt gtcgtaatac taatgaacca agttgattgc caatcataaa
     1201 atatggtaaa tctggatacc gagatgccat aatatttcgc aagcgatgca tatcttcaac
     1261 taatgtcttt gttgcatcac caatcccaaa ataaccctgc tcataattat ataatgaagt
     1321 cccatgaccc ggaaaatcac taacaacgac aacaaaacca tcgtgagcta aatattcagc
     1381 aaaccgttca tagcgccctg aatgctctcc cattgcatga tgaatctgga taatagcctt
     1441 atagcgaatc attacagtcg gtaaatatat atcaaaattg acatgtgttg ccgttgctga
     1501 tgtaaatttt tcactatatt tcatagctaa tttctccttt tcttttgata gttattttta
     1561 gcctgataag agcgctgatt attattgcga cgaggtcgaa ttaaacaatt cttcccaaac
     1621 ccaattaaat cttttcggtt tgcctgcact aaagcttcat acactaaatc ataattcttt
     1681 ggattacgat attgtatcaa tgccctttgc attgctttct cttttgccgt ctttggaacg
     1741 tatacttcag acatatcacg aggatctaaa ccagtataat acattgtcgt agataaagtt
     1801 ccaggagtcg gataaaaatc ttgaacctgt tctggctgat gatgaatatc ccgtaaatat
     1861 tctgctaatt caatcgcatc atcaagtgta cacccaggat gtgatgacat tagatacgga
     1921 actaaaaatt gttttttgtt gtattcttca tttaattgat aatatttatc aacaaatttc
     1981 tcatatagtc ctctccgagg ttttcccatc ttatctaata cctgatcact aatatgttcc
     2041 ggagctactt ttaactgacc actaatatga ttttgaacta actccttaaa aaaagtatca
     2101 tctttatcat acattagata atcatatcta attcctgaac gaacaaaaac cttttttacg
     2161 tttggtaata ctcttagttt cctcaataat tctaaataat cctgatgact tacttctaga
     2221 ttattacaag ctttaggcca taaacactgt ttagtcggac atgcaccatg tttattttgc
     2281 ttagaacaag caggccctct aaagtttgca gtaggtcctc caacatcatg aatatatcct
     2341 ttaaattcag gatcccaaac cattttgcta gcttcactaa taattgattc atgcgaacgc
     2401 gcttgaacaa ttcttccctg atgaaaagcc aatgcacaaa agttacaaga accaaagcat
     2461 cctcgattac taattaaaga aaacttaacc tcatcaattg caggaacatg ctgcttataa
     2521 ctaggatgac tatcacggtt ataagcatat gaataagcac gatcaaactc tctttgcttc
     2581 aacggacgat taggcctatt ttgaacaaca taccatcctt gatacggttc tactaaagtt
     2641 tttgcattaa aatgatcagt atttaaatac tgaatatgaa aagattttgc ataagctttt
     2701 ttatcattac aaatttcctc atagcttggc aacataactg gctcgtgagc atcatcgaga
     2761 tttttcgttt taaaaacagt tccatctaaa taagttaaat attttatttc caagccgcta
     2821 tcaagtgctt cagctacttc aataatgcta ttttcaccca ttccatacaa taataaatct
     2881 gcattagcat caataattat cgatttacgt actttgttgt cccaataatc ataatgtcct
     2941 aaacgtctta aactagcctc aatcccacca ataataatat tagcatcttt ataagcttgg
     3001 cgtgccattt gactatacac gattacagcg cgatctggtc ttttaaagcc ttcacttccg
     3061 ggtgtatata aatctttatg acgacgtttt ttagctactg tataatgatt aaccattgag
     3121 tcaatatttc ctgatgaaat caaaaaacct aatcgtggtt tgccaaattg tctaaattct
     3181 tcaacattat gccaatccgg ctgtgctaaa aaacatactt taaacccgtg actctctaaa
     3241 gttcggcaaa ttattgctgc accaaatgaa ggatgatcaa
//
LOCUS       DAOMTA010000273         3277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1129_length_3277_cov_2.488516, whole genome shotgun sequence.
ACCESSION   DAOMTA010000273 DAOMTA010000000
VERSION     DAOMTA010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1129_length_3277_cov_2.488516"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PLK68_08140"
     CDS             <1..1234
                     /locus_tag="PLK68_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRM90411.1"
                     /translation="FSQANNFYIEQAQEMEVPITFMDEEDISLFISDAQKADVVIDAM
                     FGFGLNGEVRGVARILIEEINNLYDIDIIAIDIPTGLNPDTGIPYGNVICASKTITLT
                     AIKQAFLNEECHMYTGEIAVEILDAKDLRQEQGLAKLVSPSWIKYHLKPRAYYGHKGI
                     YGKILHLTGCDYYRGAALLASKASVYSGSGVVCVCSSEKVIDALATVTPECTSKLRNS
                     KLEQELFYDRDAILIGSGLGLNEQSEQYVIDTLQYANCPIVIDADGLTIAAKHLDLLK
                     ECPVPIILTPHFGEFKRLCAYDDELDMIDKVGGFARKYGVTVVLKGPNTLITDGRETY
                     RNITANKAMATAGMGDVLAGMIVSFVGQGYTPKNAAILGTYLHGSCGDVIGDNSYTVL
                     PSKLIDLIPQVMHEIINE"
     gene            complement(1487..>3277)
                     /locus_tag="PLK68_08145"
     CDS             complement(1487..>3277)
                     /locus_tag="PLK68_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HRM90412.1"
                     /translation="KIEMQDIPFNLAYENQDSDLQQFLEISWKNIDKNIQRENAFDNI
                     LRQLGTFFNAQNVLLFHQSDNQQKFSLTSKWNYNNQISNIKNTHFSKEHFENIVSAVA
                     PQKYFVILDKNHFCYKNITELYFEKDVPLPLLFIGIYNKNKLVSCLLVETVKQHINAS
                     KSIEVIADVICKITTIYNLQTKYTYALTHDQKTGLSNYDSYIKTIRNINTDIYSTFGM
                     IEITIINLKKYNQRYGIVQGDEILQFIAQTITKFFDKKSCYRISNAHFIVLCPDITYE
                     NFIHIYDTFYQELIEFYDQWIICEKVWEKNSLVLETMQEQLEEKTRHAVNKKIHSKLA
                     KSDQSIAKILERLNTAFVNGKFLTFLQPKAHTKTNQICGAEALIRYNDPQKGIIPPGR
                     FLPEIERAGLIRHIDLFILKDVCRILSNWLKTTWKPFPISINYSRATILEPGILEETN
                     KIVESYNIPKELIEIEVTESIGSIDSASLKNIVNQFLAEGYKIALDDFGAEYSNIYIL
                     YSLHLSSLKLDRKIVSNIYHDHRAKLVIKNIINTCQQLDITCVAEGVETKEQLKVLKD
                     LSCDVIQGYYLNKPLSEDDFKKISGITNPH"
BASE COUNT         1094 a          427 c          562 g         1194 t
ORIGIN      
        1 attttctcaa gcaaataatt tttatattga gcaagcgcaa gagatggaag ttccaattac
       61 ttttatggat gaagaagata tatctttatt tataagtgat gcacaaaaag cagatgtggt
      121 aattgatgca atgtttggtt tcggattaaa tggtgaagtt cgtggagtag ctaggatatt
      181 aattgaagaa attaataatt tatatgatat agacatcatc gcaattgata ttccaactgg
      241 attaaatcct gatacaggaa taccttatgg taatgtgatt tgtgctagta agacaattac
      301 tttaacagca atcaaacagg cttttttaaa tgaagagtgt catatgtata caggagagat
      361 tgcagttgaa atattggatg ctaaagattt acgtcaggag caggggcttg ctaaattggt
      421 tagtccatca tggatcaaat atcatttaaa accgcgtgct tattatggac ataaaggaat
      481 ctatggcaag attttacatt taactggatg tgattactat cgaggagctg ctttattagc
      541 gtcaaaagcc tcggtttata gtggaagtgg tgttgtatgt gtttgttcaa gtgaaaaagt
      601 aattgatgcg ctagcaacag ttactcctga atgtacttct aaacttcgta atagtaaact
      661 ggaacaagaa cttttttatg accgtgatgc aattttgatt ggttcaggtt tagggttaaa
      721 tgaacaaagc gagcagtatg taattgatac tttacaatat gccaattgtc caatcgtaat
      781 tgatgctgat ggtttgacga ttgcagctaa acatttagat ttattaaaag agtgccccgt
      841 tcctattatc ttaacacctc attttggtga atttaagcgt ttgtgtgctt atgatgatga
      901 attagatatg attgataaag ttggtggttt tgctcgaaaa tatggtgtta ctgttgtttt
      961 aaagggtcct aacacattaa ttacagatgg tcgagagaca tatcgtaata ttacagctaa
     1021 taaggcgatg gctacggctg gaatgggaga tgttttagct gggatgatcg ttagttttgt
     1081 tggacaaggc tatacaccta aaaatgcagc tattttagga acttatctac atggcagctg
     1141 tggggatgta ataggggata attcttatac agtattacct agtaaattaa ttgatttgat
     1201 tccacaagta atgcatgaaa ttatcaatga ataattagaa cttgaaatct aaatttaatt
     1261 ttaatagatt taaaaatata tgtgaaaaag tcatcaatag tgatgacttt tataattagt
     1321 atttaagcaa gataaaagaa tgtattaaaa tcaataaaat agaaattttt tgatacaggg
     1381 tatttaacta tgatgcattt ataaaaaaag ataatcacgt aaattagttt tatgctcatc
     1441 tgctttagaa ctaaaaaact atcttaggat tatctttgtt ttagtgtcaa tgggggttag
     1501 tgatgccaga aatctttttg aaatcatctt cagataaagg cttattaaga taatagcctt
     1561 gaataacatc acaagataaa tcttttaaca cttttagctg ttctttagtt tctactcctt
     1621 cagcgacaca agttatatct aattgttgac acgtattaat gatatttttg attactaatt
     1681 tagcacggtg atcatgataa atattgctta cgatctttcg atcaagttta agtgagctta
     1741 agtgcagcga gtatagtata taaatattgc tatattcagc accgaagtca tctaaagcaa
     1801 ttttatatcc ttcagctaaa aattgattaa caatattttt taaactagca gaatcaattg
     1861 atccaatgga ttcagtaact tctatttcaa ttaattcttt aggaatatta tagctttcaa
     1921 cgattttatt agtttcttct aaaataccag gttctaaaat agttgctcga gaataattta
     1981 ttgaaattgg gaaaggcttc caagtagttt ttaaccagtt actgagaatt cggcagacat
     2041 cttttagaat aaataaatct atatgtctaa ttaatcctgc tctttctatt tctggaagaa
     2101 aacgccctgg tggaataatt cctttttgag gatcgttgta tctgatcaat gcttcagcac
     2161 cacaaatttg attagtttta gtatgagcct taggttgtaa aaaggttaaa aattttccat
     2221 ttacaaaagc agtatttaaa cgctctaata tttttgctat cgactgatca gatttagcta
     2281 acttagagtg tattttttta tttacagcat gtcttgtttt ttcctctaat tgctcttgca
     2341 ttgtttctaa cacaagtgaa ttcttttccc aaactttttc acaaatgatc cattgatcat
     2401 aaaattcaat tagctcttgg taaaaagtat catatatatg aatgaagttt tcatacgtaa
     2461 tatctggaca aagcactata aaatgggcat tactgatccg ataacatgat tttttatcaa
     2521 aaaatttagt aattgtttgt gcgatgaact gtaagatttc atcaccttgg acgatgccat
     2581 accgctgatt atattttttt agattaatga tagttatttc aatcattcca aaagtagaat
     2641 aaatatcagt atttatattt cttatagtct taatataaga atcgtagtta gataaaccgg
     2701 ttttttgatc atgagtaagg gcataagtat atttagtctg caaattataa atagtggtaa
     2761 ttttacaaat aacatcagca atcacctcaa tagattttga agcgttaata tgttgtttaa
     2821 cagtttccac gagcaagcat gatacaagct tgtttttgtt ataaatgccg ataaataata
     2881 atggtagtgg tacatccttt tcaaaatata gttctgtaat atttttataa caaaaatggt
     2941 ttttatctaa tattacaaag tatttttgtg gtgcgacagc actaacaata ttttcaaagt
     3001 gttccttact gaaatgagta tttttaatat tagagatttg attattataa ttccactttg
     3061 aagtcagaga aaatttttgt tgattatcgc tttgatgaaa taagagaaca ttttgggcat
     3121 taaagaatgt tcctaattgt ctaagaatat tatcaaaagc attttctcgt tgaatatttt
     3181 tatctatatt tttccagctt atttctaaaa attgttgtaa atctgaatcc tgattttcat
     3241 atgctaagtt aaagggaata tcctgcattt caatttt
//
LOCUS       DAOMTA010000274         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1135_length_3271_cov_2.302550, whole genome shotgun sequence.
ACCESSION   DAOMTA010000274 DAOMTA010000000
VERSION     DAOMTA010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_3271_cov_2.302550"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLK68_08150"
     CDS             <1..316
                     /locus_tag="PLK68_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM90413.1"
                     /translation="LICLSRKFGEGFYHDFVEAFNKDKIEPNIIKEVDTLDELILSVK
                     LNEGIGLTSREVVNEEEVAILDIINSHHHANYVIGYARDINNQFIFDFVALAKRYFNK
                     TL"
     gene            407..1342
                     /locus_tag="PLK68_08155"
     CDS             407..1342
                     /locus_tag="PLK68_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRM90414.1"
                     /translation="MSKVIVTGVDGNFGGYVARNITQLKEKEDLIFTCPFEEGLKEFK
                     DTGIDCRVANFNHPDEQLVEAFKGGDTILIISAPFVGAKRQAAHKNAIDAAIKAGVKK
                     VVYTSLVNARDVENPSIEKIDHAWTEEYIESTELDYIFLRNSQYAEAMITSYLTSNGN
                     LLSCQGDGKMAYISRLDCAMAAMYALTKDDMHKQVLNINGPELLTLHEFAAIGNRETG
                     MDVKVVDVSEEEVYAGFDAIGVPRTTDGAFKDGSPAPYSSDGMVTFARAIRIGKMDNF
                     TDDFEKLTGVKPRTVAYMFANNSEYGVGARNSTDD"
     gene            1505..1978
                     /locus_tag="PLK68_08160"
     CDS             1505..1978
                     /locus_tag="PLK68_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HRM90415.1"
                     /translation="MKLLFKQRFFSWLDSYDIYDEMGNTVYTVKGELSWGHKLQIYNA
                     DGLPVGTIKEEVFTFLPKFAMYLNDEYIGQIKKELTLFKPSFILDYNDWKISGNFWEW
                     DYEIIDSRGIVIGDINKELFNFTDTYSLTISDPRNAIYVLMIALAIDAQKCSNNS"
     gene            2067..>3271
                     /locus_tag="PLK68_08165"
     CDS             2067..>3271
                     /locus_tag="PLK68_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM90416.1"
                     /translation="MGKDENNFARGSIPRHIINLAGPMIVAQLINVLYNVIDRIYIGR
                     IPDVATLAMGGLGLCLPLISIIIAFANLFGMGGAPLCSIARGRGDIEEAEEIMGNSFM
                     LLIIFGIVLTVIGLIFKEDLLWLFGASEHTIGYANDYMTIYLLGTVFVLIGLGMNSFI
                     NSQGFAKIGMMTVLLGAIVNIILDPIFIFGLDLGVKGAAFATVISQFISALWTLRFLT
                     GKKTILKIKKQYLSLKVKYVTKIISLGMAGFMMAITNSIVTIVCNATLQQYGGDLYIA
                     IMTIINSIREVASLPGQGMANACQPVLGFNYGAKEYARVLQGIKFVTLTALSMMLIVW
                     LAITVFPELFIKIFSHNQEIITHGVSALRLYFFGFFMMSFQMTGQAAAVGLGKSKQAV
                     FFSIFRKVI"
BASE COUNT         1113 a          373 c          651 g         1134 t
ORIGIN      
        1 gctaatttgt ttatcaagaa agtttgggga aggcttttat catgattttg tagaagcttt
       61 taataaagat aagattgagc caaatattat aaaggaagta gatactcttg atgagttgat
      121 tttatcagtt aagcttaatg aaggaattgg tctaacttca cgtgaagttg ttaatgagga
      181 agaagtagcg attttagata ttattaattc gcatcatcat gcaaactatg ttattggtta
      241 tgctagggat ataaataatc aatttatttt tgattttgtt gcgctggcga aaagatattt
      301 taataaaact ctataagtaa ttcttataaa gtttataaga ttatagctac tattttttat
      361 aattatctag ctctataata ttaatatata gatggaggaa gataagatgt caaaagtaat
      421 agtaacaggt gtagatggta attttggagg ctatgtagcg agaaatatta ctcagttaaa
      481 agaaaaagaa gatttgattt ttacttgtcc atttgaagaa ggactaaagg aatttaaaga
      541 tactggcatt gattgtcgag tagcaaattt taatcatcca gatgaacagt tagttgaagc
      601 cttcaaaggt ggcgatacaa ttttaataat ttcagcacca tttgttggag caaagcgtca
      661 agcagcacat aaaaatgcaa ttgatgcggc aattaaagca ggggtaaaaa aagttgttta
      721 tacatcatta gtaaatgctc gtgatgtaga aaatcctagt attgaaaaaa tcgatcatgc
      781 atggactgaa gaatatatcg aaagtactga attagattat attttcctta gaaattcaca
      841 atatgctgaa gcaatgataa ctagttattt aacatctaat ggtaacttat tgagttgtca
      901 aggagatgga aagatggcat atatctcacg tttagattgt gcgatggcag cgatgtatgc
      961 actaacaaaa gatgatatgc ataagcaagt tttaaatatt aatggtcctg aattattgac
     1021 tttacatgaa tttgcggcaa ttggaaatag agaaacagga atggatgtaa aagttgtcga
     1081 tgttagtgaa gaggaggtat atgctggatt tgatgctatt ggggtaccac gtactactga
     1141 tggagcattt aaagatggct caccagctcc ttattcaagt gatggaatgg taacttttgc
     1201 aagagcaatc cgaattggta aaatggataa ttttactgat gattttgaaa aattaactgg
     1261 tgtaaaacca cgtacagtgg cttatatgtt tgccaataac agtgaatatg gtgtcggagc
     1321 aagaaattct acagatgact aattattagc ttattaattg gtagtgatat tattgagaaa
     1381 tcaataatat tactatcaat ttttatttga tagaatatgt caaataaaat accaagatta
     1441 tttaagaatt tatttaaaga aatagaatta ttttatgcta tgataaaatt atggaggcgg
     1501 aaaaatgaaa ttattattta aacagcgctt tttttcatgg ctagatagtt atgatattta
     1561 tgatgaaatg ggaaatacag tatacactgt aaaaggtgaa ttaagctggg gacataaatt
     1621 acagatatat aatgctgatg gtttacctgt tggtacaatc aaagaagaag ttttcacttt
     1681 tttacctaaa tttgcgatgt accttaatga tgaatatatt ggtcaaataa aaaaagaact
     1741 aacattattt aaaccatcat tcattttaga ttataatgat tggaaaattt ctggtaattt
     1801 ttgggaatgg gattatgaaa tcattgattc tcgtggtatt gttattggag atattaataa
     1861 ggaattattt aattttacgg atacttattc tctaaccatt agtgatccac gaaatgcaat
     1921 ctatgtttta atgattgctt tagctattga tgcgcaaaaa tgttcaaata acagttaata
     1981 agacacgaga gtgtcttgtt tcatcttatg aaaaataatt actaaattaa tgaagaggtt
     2041 tataataata accgaaggtg gtaaaaatgg ggaaagatga aaacaatttt gcgcgtggca
     2101 gtattccacg acatattata aatttagctg gtccaatgat agtggcacag ttgatcaatg
     2161 ttttatataa tgttattgat cgtatttata ttggaagaat tccagatgtt gcgacattag
     2221 ctatgggagg actagggcta tgtttgccac tgatttctat tattattgct tttgctaatc
     2281 tatttggtat gggcggtgct ccactttgtt caattgctcg aggacgtggt gatattgaag
     2341 aagctgaaga aataatgggt aattctttca tgctattaat tatttttggg attgttttaa
     2401 cagttattgg tttgatcttt aaagaagatt tgctgtggct ctttggtgcc agtgaacata
     2461 caatcggtta cgctaatgat tacatgacta tctatttatt agggacagtt tttgtactga
     2521 ttggtttagg tatgaatagt tttattaata gtcagggatt tgctaaaatc ggaatgatga
     2581 ctgttttgct gggagcaata gttaatatta ttttagatcc aatttttatt tttggactag
     2641 atttaggggt taaaggagct gcttttgcaa cagttatttc acaatttatt tctgcattat
     2701 ggacattacg ttttcttaca gggaaaaaaa caatattaaa gataaaaaaa cagtatttga
     2761 gtttaaaagt aaaatatgtc acaaagatta tttccctagg aatggcaggg tttatgatgg
     2821 caatcactaa ttcgattgta acaatcgtat gtaatgcgac attgcaacaa tatggaggag
     2881 atctttatat tgcaattatg acgatcatta attcaattag agaggttgct tctttgccgg
     2941 ggcaaggaat ggcaaatgct tgtcaaccag tattaggatt taactatggt gctaaagagt
     3001 atgcacgggt tcttcaagga attaaatttg taactttaac ggcactatca atgatgctga
     3061 tagtttggct ggcaataacg gtatttcctg aattatttat taagatattt agtcataatc
     3121 aagaaatcat tacccatggc gtgagtgctt tacgtttgta tttctttggc tttttcatga
     3181 tgtcatttca gatgacagga caagcagcag cagttggatt aggaaagtca aaacaagctg
     3241 ttttcttttc aatttttaga aaagtaatta t
//
LOCUS       DAOMTA010000275         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1146_length_3252_cov_1.961526, whole genome shotgun sequence.
ACCESSION   DAOMTA010000275 DAOMTA010000000
VERSION     DAOMTA010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_3252_cov_1.961526"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>217)
                     /locus_tag="PLK68_08170"
     CDS             complement(<1..>217)
                     /locus_tag="PLK68_08170"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRM90417.1"
                     /translation="DSPLTELGIKQAKVAEKWFKDHDITFDHAYCSTSERCSDTLELV
                     TDMPYTRLKGLKENFYGQLEGESERLNC"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<228..338)
                     /locus_tag="PLK68_08175"
     CDS             complement(<228..338)
                     /locus_tag="PLK68_08175"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRM90418.1"
                     /translation="MVKKLYLMRHGQTLFNLQNKIQGWCDSPLTELGQYQA"
     gene            complement(352..1329)
                     /locus_tag="PLK68_08180"
     CDS             complement(352..1329)
                     /locus_tag="PLK68_08180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM90419.1"
                     /translation="MEYVKLGNTGLEVSKICLGCMSFGDPQRWIHSWVLNETDSRKII
                     KKALDLGINFFDTANVYGLGASEEILGRALQEYAKREEIVIATKVSGQMHEGPNGKGL
                     SRKAILHEVEQCLKRLGTDYIDLLYIHRWDYNTPIEETMCALNDLVRAGKVHYLGASS
                     MYAWQFQKAQYLAEKNGWTKFSVMQGHYNLLYREEEREMIPLCKDMKVALVPYSPLAA
                     GRLTRDWNSDSKRAQEDLVAKRKYDSTVDTDRKIVERVAEIADKYQVTKSQVAVAWLW
                     AKGVTAPIVGITKEKYLDDFAGALTVKLTQEDIEYLEECYQPHVIMGHN"
     gene            complement(1344..1754)
                     /locus_tag="PLK68_08185"
     CDS             complement(1344..1754)
                     /locus_tag="PLK68_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM90420.1"
                     /translation="MNREESLGPSAVFELGEPNDAYAKYFIGQSYLKHLTTEGVGVVN
                     VTFEPGCRNNWHIHHKGGQILIVTGGKGYYQEWGKPVQCLKPGDIVNIPPEIKHWHGA
                     VKDSWFSHLAVEVPAKDASNEWCEPVTDEEYEKL"
     gene            complement(1772..2473)
                     /locus_tag="PLK68_08190"
     CDS             complement(1772..2473)
                     /locus_tag="PLK68_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRM90421.1"
                     /translation="MNKKAEQLFSDLNDGKDFLVKDPELREIMINYLYGDVYHHGNLD
                     FKLRELILIVVNTTNHTLKALKEHVTSGLSVGVTPVEIKEAVYQCTPYIGLGKVEEAL
                     EIVNLVFEEKNISLPLLPQATVQGDNRFEKGFAVQSAAFGQEHIQAGHDHAPKELKHI
                     QNYLSEYCFGDFYTRNGLDLPTRELITMVMLATLGTVGNNRDTLIETITQCQPYIGFP
                     RTLNAIAIINEITKK"
     gene            complement(2487..>3252)
                     /locus_tag="PLK68_08195"
     CDS             complement(2487..>3252)
                     /locus_tag="PLK68_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM90422.1"
                     /translation="AGSICSPILSSFIENFGWQTGYLIKAAIILGLCLPAVLYPFHLD
                     PQKDGCLPYGHVEQPQDMVTQVSNSFSFTTVAFISFFVFGLLCSCITSVTQHLPGYGQ
                     SLGYSAALGAMLLSSGMVGNIISKLIIGVLSDHLGAIKATVTMIIANTVGIILLMWGS
                     TAWLLIIGAFLFGSCYSIGAVALPLLTKYFFGIDNYARVFPKISFASNLGAAISLSMV
                     GYIYDFFGSYLYAFIIALAMIAVCVITLTLTAKTKE"
BASE COUNT         1028 a          677 c          413 g         1124 t
ORIGIN      
        1 gacaatttaa cctttcactc tcaccttcta attgaccata aaaattttct tttaaccctt
       61 ttaaacgcgt atacggcata tctgtaacta attctaaggt atcactacat ctctcactag
      121 tagaacaata ggcatgatca aaagtaatat catgatcttt aaaccatttt tccgcaactt
      181 ttgcttgttt gattcctaat tctgtaagtg gtgaatcnnn nnnnnnnagc ttgatactgc
      241 cccaactctg ttaatggcga atcacaccag ccttgaattt tattttgaag attaaatagt
      301 gtttgtccat gacgcattaa atataatttt ttcaccattt tttcatcctt tctaattatg
      361 tcccataatt acatgaggct gatagcattc ttctaaatat tctatatctt cttgtgttag
      421 tttaactgtt aaagcacccg caaaatcgtc taaatatttt tcttttgtaa tccctacaat
      481 tggtgcagtc acgcctttag cccataacca tgcaacagca acttgagatt tagtgacttg
      541 atatttatct gcaatttcag caactctttc aacaattttt cgatctgtat ctacagtact
      601 atcatatttt cgttttgcaa ccaaatcttc ttgagctctt tttgaatctg aattccaatc
      661 acgagttaat cgtcctgccg ctagtggact ataaggcacc aaagctactt tcatatcttt
      721 gcataaagga atcatttctc gctcttcttc acgatataat aaattataat gaccctgcat
      781 tacagaaaat ttagtccatc catttttttc tgcaagatac tgtgcttttt gaaattgcca
      841 ggcatacatc gatgaagcac ctaaataatg aacctttcct gctctaacca aatcatttag
      901 agcacacatc gtttcttcaa ttggagtatt ataatcccag cgatgaatat aaagtaaatc
      961 aatataatcg gttcccaatc tttttaaaca ttgttcaact tcatgcaaaa ttgcttttcg
     1021 cgataatcct ttcccatttg gtccttcatg catttgaccg cttacttttg tagcgattac
     1081 aatttcttcc cgtttagcat actcctgaag cgctcgtcct aaaatttctt cactggcccc
     1141 taatccataa acattagcag tatcaaaaaa attgattcct aaatctaatg ctttttttat
     1201 aatcttacga gaatcagttt cattcagtac ccaactatgg atccaacgtt gaggatcccc
     1261 gaaactcata caacctaaac atattttaga tacttctaaa cctgtatttc ctaattttac
     1321 atattccatt atcttatctc cttttataat ttttcatatt cttcatctgt aactggttca
     1381 caccactcat tagaagcatc cttagccggc acttcaacag ctaaatgact aaaccagcta
     1441 tcctttactg ctccatgcca atgttttatc tcaggtggaa tatttacaat atcacctggt
     1501 tttaaacatt gtactggttt tccccactct tgataataac cttttccacc agtaacgatt
     1561 aagatttgac cacctttatg atgaatatgc caattattac gacaaccggg ttcaaatgtg
     1621 acattgacaa caccaactcc ttctgttgtt agatgtttca aataactttg accaataaaa
     1681 tattttgcat acgcatcatt aggttctcct aattcaaata ctgcagatgg tcctaaactt
     1741 tcttcacgat tcattttaca ttcctccatt tttatttctt agttatttca ttaataattg
     1801 caattgcatt taatgttctt ggaaatccta tatacggttg acattgagtg attgtttcaa
     1861 ttaaagtatc acgattattt ccaactgttc ctaaagtagc taacatcacc attgtaatca
     1921 attctcttgt tggtaaatcc aaaccatttc ttgtataaaa atctccaaaa cagtattcag
     1981 ataaataatt ttgaatgtgc tttaattcct ttggagcatg gtcgtgacct gcttgaatat
     2041 gctcttgtcc aaatgcagca ctttgaactg caaatccctt ttcaaatcta ttatcacctt
     2101 gtaccgttgc ttgtggtaat agtggtaaac taatattttt ctcttcaaaa acaagattaa
     2161 ctatttctaa tgcttcttct actttgccta atcctatata gggagtgcat tgatatactg
     2221 cttctttaat ttctacaggt gttactccga cactcaagcc acttgtcaca tgttccttta
     2281 aagcctttaa agtatgattg gtagtattaa cgacaattaa aataagctct cttaatttaa
     2341 aatcaaggtt accatggtga tagacatccc cataaagata attaatcata atttcccgaa
     2401 gttctggatc ctttactaaa aaatcctttc catcatttaa atcactaaat aactgttctg
     2461 ctttcttatt cataatcttt cctcctctat tcttttgttt tggcagttaa tgttaaagta
     2521 attacacaaa ctgcaatcat tgctagagca atgataaatg catataaata agagccaaag
     2581 aaatcatata tataccctac catagataaa gaaattgctg ctcctaaatt agaagcaaaa
     2641 gaaatctttg ggaaaactct tgcataatta tctattccaa agaagtattt ggttaataaa
     2701 ggcaatgcca ctgctccaat tgaataacat gaaccaaaca aaaatgctcc aataattaat
     2761 agccacgctg ttgaacccca cattaataaa ataattccta cagtattagc tatgatcatt
     2821 gtaacagttg ctttaattgc ccctaaatga tcacttaaaa ctccaataat caatttggaa
     2881 ataatatttc ctaccattcc cgatgacaaa agcattgctc ctaatgcagc actatagccc
     2941 aatgattgac catatccagg taaatgctga gtaacactag taatacaact acataataat
     3001 ccgaagacaa agaaactaat aaatgcaact gttgtaaagc taaatgaatt tgacacttgt
     3061 gtcaccatat cttgtggctg ctccacatgt ccataaggta aacaaccatc tttttgtgga
     3121 tcaaggtgaa atggatataa gacagcgggc aaacataatc ctaatataat tgcagctttg
     3181 attaaataac cagtttgcca tccaaaattt tcaataaagc ttgataaaat cggtgaacaa
     3241 atcgatcctg ct
//
LOCUS       DAOMTA010000276         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1149_length_3246_cov_2.494829, whole genome shotgun sequence.
ACCESSION   DAOMTA010000276 DAOMTA010000000
VERSION     DAOMTA010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1149_length_3246_cov_2.494829"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2428)
                     /locus_tag="PLK68_08200"
     CDS             complement(<1..2428)
                     /locus_tag="PLK68_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM90423.1"
                     /translation="MKRILKGALVALMVVSSTIGVKAESKISAEVQRIIDSMTPVEKV
                     AQMIQADTRWITPAEVAEYKIGSILSGGGAAPTSGNELSNWVTSANSYQKAVIDSGGI
                     PLLYGIDAVHGNNNLYGATIYPHNIGLAAANNTQLVEQIGEATASEVRAMGANWTFTP
                     TLGVPHNERWGRTYETFGDDVERVTNLGTAYIKGIESDGDTLSSAKHYLGEGLTTNGA
                     NQGNVELSDADYQDLINANMNNPMVKELLTPYKQAIKQGTKSIMVTYNSINGKRCHGN
                     KDVVTTLLKENLSFDGIVISDYNGLDQIENQATYKDKAIACINAGVDVLMVAEKDGST
                     PRWKNLYNALVEAVNEGKISEERLNDAVARILTAKEELGFLDNPSKAYADEADQAKFG
                     GSEHRALAKQAVSESLVLLKNDEVNAGKTVMQALADMDNIVVAGSAGDDIGKQCGGWT
                     ITWQGATGNTTPGTTIFSGLKAAMDKKGGTISYNANGVFTNSDNKVDAAIVVVGEDPY
                     AESNGDRSAGQLKLPANDISTIKRIENSHPDLPIILVLTTGRPIAMADYVNDDHIKGI
                     VNAWLPGSEGDGVADVLLGDKDFVGTNPITWTWYPQDITSKYDDSSKVLYPVGYGLKK
                     AQKTGDFTAPADPNVIDLAKTNGKLEAEAFVDAHSEIKLENNGTTVGYLQDGRHMTYK
                     INVPEQAAYKLTVQAAREYAATIDGAFELYLDDELVLEKKNTPIVSTGSWTKFTAQEM
                     TALVSLKAGIHELKLVARDKDFNIDYYIFEKAGDYVGPIIPDEVTNEGTGAMLQEGAV
                     EVSMSSSENSQ"
     gene            2584..>3246
                     /locus_tag="PLK68_08205"
     CDS             2584..>3246
                     /locus_tag="PLK68_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM90424.1"
                     /translation="MLKYEMIVNDLQKRIMNEEFEETRKLPTEEKLIEEYGVSRNTIR
                     NAIKILMNLGIIYPVQGSGMFVRAPKKKGTVYLNSTRGVTMDNPGNKIINKLLDIQIV
                     EADEKLSEQMNCKVGTPVYYLKRLRIVDGIPYALERTYYNKEIVPYLGKEIAEGSIFN
                     YLKDDLKISFGFADKYLTAIKLSKEDAALLELEENDPAIMINDNIHLSNGQLFNTSNI
                     IYN"
BASE COUNT         1011 a          657 c          459 g         1119 t
ORIGIN      
        1 cttgtgaatt ttcagaagaa gacatgctta cttcaactgc tccttcttgt aacattgctc
       61 cagttccctc atttgttact tcatcaggaa tgattggacc aacataatca ccagcttttt
      121 caaaaatata ataatcaata ttaaaatctt tatcgcgtgc tacaagttta agttcgtgta
      181 tccccgcttt taaactaacc aaagctgtca tctcttgagc tgtgaactta gtccagctgc
      241 ccgttgaaac aattggtgta tttttctttt ctagtaccaa ttcatcatct aaatataatt
      301 caaatgctcc atcaattgtc gctgcatatt cacgtgctgc ttgaacagtt aatttatatg
      361 ctgcttgttc tggcacatta attttataag tcatatgccg tccatcttgt aaatacccta
      421 cagttgtacc attattttct aacttaattt cactgtgtgc atccacaaac gcctcggctt
      481 ctaattttcc attagttttc gctaaatcta tcacattagg atcagctggt gctgtaaaat
      541 caccggtttt ttgagctttt ttcaatccat atccaaccgg atataatact ttgcttgaat
      601 catcatactt actagtaata tcttgaggat accatgtcca agtaattgga tttgttccta
      661 caaaatcttt atcacctaat agtacatcag caaccccatc accttcacta cctggtaacc
      721 atgcattaac aatccctttg atatgatcat cattaacata atcagccatt gcaattggtc
      781 gccctgttgt taaaactaaa ataatcggta aatcaggatg actattttca atccgtttaa
      841 ttgtactaat atcattagct ggtaatttta attgtccagc actgcgatca ccattacttt
      901 cagcatatgg gtcttcacca actacaacta ttgctgcatc aactttattg tccgaatttg
      961 taaatacacc attagcatta tagctgattg ttccaccctt tttatccatc gctgctttta
     1021 aaccactgaa aatagttgta ccaggtgttg tatttccagt tgccccttgc caagtaattg
     1081 tccaaccacc acattgttta ccaatgtcat caccagcact accagcaacc acgatattat
     1141 ccatatctgc taaagcctgc atcactgttt taccagcatt aacttcatca ttttttaata
     1201 aaactaaaga ttcgctaact gcttgtttag ctaatgcccg atgttcacta ccaccaaatt
     1261 tagcttgatc tgcctcatca gcatatgctt ttgaaggatt atctaaaaat cctaattctt
     1321 ctttagcagt tagaattctt gcaactgcat catttaagcg ttcttcacta atttttcctt
     1381 cattaacagc ttcaactaaa gcattatata aatttttcca gcgtggtgtt gaaccatctt
     1441 tttctgctac catcaagacg tcaacaccag cattgataca agcaattgcc ttatctttat
     1501 aagttgcttg attctcaatt tgatcaagac cgttataatc gctaataaca atgccatcaa
     1561 aactaagatt ttcttttaat aacgttgtaa caacgtcctt atttccatga catcttttac
     1621 cattgatact attataggta accataattg atttagttcc ttgtttaata gcttgtttat
     1681 aaggtgttaa aagttcctta accattggat tattcatatt tgcattgatt agatcttgat
     1741 aatcagcatc actaagctct acattacctt ggttagctcc attagtagtt aatccttctc
     1801 ctaaataatg ttttgctgac gatagagtat caccatcact ttcaattcct ttaatatatg
     1861 cagtacctaa attagttacc cgttcaacat catcaccaaa agtttcatat gtacgccccc
     1921 aacgttcgtt atgaggtaca cctaatgttg gtgtaaatgt ccagttggct cccattgctc
     1981 taacttcact tgcagtagcc tccccaattt gttcaactaa ttgagtatta ttagctgctg
     2041 ccaatccaat gttatgagga tagatagtcg ctccatacaa attgttattt ccatgaaccg
     2101 catctattcc atataacaat ggaataccac cactatcaat tactgctttt tgataactat
     2161 ttgctgaagt tacccaatta cttaattcat ttccactagt tggtgctgct cccccaccac
     2221 taagaattga accaatctta tactccgcca cttcagctgg agtgatccag cgagtatccg
     2281 cctgaatcat ttgtgcgact ttttcaaccg gtgtcatact atcaataatt ctttgtactt
     2341 cggctgaaat cttcgactct gctttaaccc caatagttga tgaaacaacc attagagcta
     2401 ctaatgctcc cttcaatatt ctcttcatct tttctcctcc aaatttattg gtacaaattt
     2461 attatattcc aatgaagaat taatgtaaag cgctttcaca ataaaaatag aataaattat
     2521 attcatacca ataaatattg tgaaacttga ctaatatgat ataatgagtt ttgaggtgat
     2581 tatatgttaa agtatgaaat gattgtaaat gatcttcaaa aaaggataat gaatgaagaa
     2641 tttgaagaga ctcgaaaatt acctactgaa gaaaaactga ttgaagaata tggggttagt
     2701 cgtaatacaa ttcgtaatgc aattaaaata ttaatgaatt taggcattat ttatccagtt
     2761 caaggaagtg ggatgtttgt tcgggcccca aagaaaaaag gaacagtgta tcttaactcg
     2821 acccgtgggg tgacaatgga taatcccggt aataaaatca ttaataaatt attagatatt
     2881 caaatagttg aagctgatga aaagttaagt gaacagatga actgcaaagt tggaactcca
     2941 gtttattact taaagcgtct tcgtattgtc gacggtatcc catatgcttt agagcgaaca
     3001 tattataata aggaaatcgt accctattta ggtaaggaaa ttgctgaagg atcaattttt
     3061 aattatttaa aagatgattt aaaaatctct tttggttttg ctgataaata tctaactgca
     3121 ataaaattaa gcaaagaaga tgctgcttta ctagaacttg aagaaaatga tccagccatt
     3181 atgatcaatg ataatattca tttatcaaac ggacagttat ttaacacttc aaatatcatt
     3241 tataat
//
LOCUS       DAOMTA010000277         3245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1150_length_3245_cov_2.369279, whole genome shotgun sequence.
ACCESSION   DAOMTA010000277 DAOMTA010000000
VERSION     DAOMTA010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1150_length_3245_cov_2.369279"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLK68_08210"
     CDS             complement(<1..325)
                     /locus_tag="PLK68_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90425.1"
                     /translation="MKEKIKKLLRSKVFNILIVIIIFFVGLKLYTNYISNLFDNSTPQ
                     KAIAEYVYNINSTYKVFFMKIEEVHSEEIEKNDECNYYMITQSIIGKPNNMGGTFKVK
                     KIDNFY"
     gene            complement(325..1386)
                     /locus_tag="PLK68_08215"
     CDS             complement(325..1386)
                     /locus_tag="PLK68_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HRM90426.1"
                     /translation="MKGRYVIKKSRKFIVSLIIAVFLVGIFPTNISTVTAMEKEISLD
                     ADCNIYSESPYNLLENIVDLKSGGVLNVNGTSNPLFWEFDNMSTAFDIIIISKFDDEL
                     NEISSVGNLDTLSLNNWKEYFNVFKEMYFDNIASGDIEDINILNQFFTVCENHEINSQ
                     ARILCNQENVDIFELINYLPYTSPIIDTINQLPMTRAASFNFNKSTAVSYAKKYAVNP
                     NPSYTTYSTDCTNFASQILRSGGAPTNTTWKPYTASWSAAHNFETYWYSRSNSQYASG
                     NFNTFSGNLQAGDFIIADWEKDGRYNHVAFVVASGSKTSSGYYDVTIAQHTSNYCAKV
                     SSSVNGWENTSGTYVRIRF"
     gene            1519..1857
                     /locus_tag="PLK68_08220"
     CDS             1519..1857
                     /locus_tag="PLK68_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRM90427.1"
                     /translation="MSRSSNNFFRMEMLLLKIIEIGNGEYYGYKIVQQLQELSDDKIK
                     LAEGVMYPILYRLLDKGYIIDEKRLVGKRKTRVYYKLEPKGKEYLNQLYIDYMDINTS
                     IIKIMEAGNE"
     gene            1850..2200
                     /locus_tag="PLK68_08225"
     CDS             1850..2200
                     /locus_tag="PLK68_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6120 family protein"
                     /protein_id="HRM90428.1"
                     /translation="MNNVVKQYLKECRRTFPFISKNEKLFFKRLEDNLNDIDENVTYK
                     EICLKYGEPKNVMISYIENCDNEYILKRTSIKSIVKKIFITLFCILIALCATLLIYNQ
                     LIYNAYDDSNTIKK"
     gene            2207..2686
                     /locus_tag="PLK68_08230"
     CDS             2207..2686
                     /locus_tag="PLK68_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90429.1"
                     /translation="MRRSKENEKIFASFLTVLTLLGTTPVFALNEESNTQIVERFEDG
                     SYIEVTIEEISTLTRSNTKKGNKTTYYKNTYDVILWSVKTSGTFTYDGSTAKCTAASV
                     DTQCPAINWKLSNIRSSKSGASAYGYAIAKSYNGLGMVLQTINETVKLTCSPSGTLS"
     gene            complement(2752..2988)
                     /locus_tag="PLK68_08235"
     CDS             complement(2752..2988)
                     /locus_tag="PLK68_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90430.1"
                     /translation="MAEDIDDVFEDIQQDILDDLKGMKDYSYPVYRVEADYIDGAADT
                     SKYDYQMLGVVKPERTAPKNHTCWHGIIETELTE"
     gene            complement(3059..>3245)
                     /locus_tag="PLK68_08240"
     CDS             complement(3059..>3245)
                     /locus_tag="PLK68_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90431.1"
                     /translation="RNNSHSGNRNKSISCKSSNGYCCQAMIINMIMNMTIYDIGELTL
                     DAAEKEIQAEWEKKKNE"
BASE COUNT         1117 a          520 c          443 g         1165 t
ORIGIN      
        1 aatagaaatt atctattttt ttgactttaa atgttcctcc catattatta ggctttccaa
       61 taatactttg cgttatcatg taataattgc attcatcatt cttttcaatt tcttcactat
      121 gaacttcttc tattttcata aagaatacct tatatgttga atttatatta taaacatatt
      181 ctgctattgc tttttgtggt gttgaattat caaatagatt gcttatataa ttagtgtaca
      241 atttcagccc gacaaaaaat ataattataa caatcaaaat attaaatact ttacttctca
      301 acaatttctt tattttttct ttcattagaa tctaatcctt acgtatgttc cagaggtatt
      361 ttcccatcca tttactgaag aacttacttt agcacaatag ttacttgtat gttgtgctat
      421 tgttacatca taatatccac tagatgtttt actcccagat gcaactacaa aagcaacatg
      481 attatatcgt ccatccttct cccaatctgc tattataaaa tcacccgcct gcaaattacc
      541 tgagaatgta ttaaagtttc ctgatgcata ttgactatta cttctagaat accagtatgt
      601 ttcaaaatta tgcgcagctg accaagaggc tgtatatggt ttccatgtag tatttgttgg
      661 tgcaccacct gaccttagaa tctgcgatgc aaaattagta caatcggtac tatatgttgt
      721 atagcttgga ttaggattaa cagcatattt ttttgcgtaa cttactgctg tgcttttatt
      781 aaagttaaat gaagcagctc gtgtcatagg caactgatta atagtatcaa ttataggact
      841 tgtgtacggt aagtaattga ttaattcaaa aatatcgaca ttttcctgat tacataaaat
      901 cctagcttga ctatttattt catgattttc acatactgtg aaaaattgat ttaagatatt
      961 tatatcttct atatctccac tagcaatatt atcaaaatac atttctttaa atacattaaa
     1021 atattctttc caattgttta agcttaatgt gtctaaattt ccaactgatg agatttcatt
     1081 taattcatca tcaaatttgc taataataat aatatcaaat gcagtactca tattatcaaa
     1141 ttcccaaaac aacggattgc tagttccatt aacatttaat actcctcctg attttaaatc
     1201 aacaatattt tctaataaat tgtaaggaga ttcactataa atattacaat ctgcatctag
     1261 gcttatttct ttttccattg cagttacagt agaaatatta gttggaaata ttcccactaa
     1321 aaatacagca ataattaaag atacaataaa ttttctactc ttttttataa catatcttcc
     1381 tttcatattt gttgaaaacg tttccttaac tatatgatag cactttattt acatatgtca
     1441 atagctacat atattttata tgtattgaat tatcgattat attgtgattt aattatatta
     1501 aagataggag atggattaat gagcagatct tcaaacaatt tttttaggat ggaaatgttg
     1561 ctgttaaaaa tcatagaaat cggaaatggc gaatattatg gttataaaat agtccagcag
     1621 cttcaggagt tatctgatga taaaataaag ttagctgaag gagtcatgta ccccatcctc
     1681 tatcggcttt tagacaaagg ttatattatc gatgaaaaaa ggcttgttgg taaaagaaaa
     1741 acaagagttt attataagct tgaaccaaaa ggaaaagaat atcttaacca actttacatt
     1801 gattacatgg atataaacac tagtattatt aaaatcatgg aggcaggaaa tgaataatgt
     1861 tgtcaaacaa tatctaaagg aatgtagacg tacatttcca tttataagta aaaatgaaaa
     1921 attatttttt aagcgcttag aagataattt gaatgatatt gacgaaaacg ttacttataa
     1981 agaaatatgc ttaaaatatg gtgaaccaaa aaatgttatg atttcttata tagaaaattg
     2041 tgacaatgaa tatatcttaa agcgaacttc tattaagtcc attgtgaaga aaatattcat
     2101 taccctattt tgtattctaa tagcactatg tgctacactt cttatttata atcaattgat
     2161 atacaatgca tatgatgatt ctaatactat aaaaaagtaa ctattattga ggaggagtaa
     2221 agaaaatgaa aaaatatttg caagtttttt aactgtttta acactattag gcactacacc
     2281 tgtatttgca ttaaatgaag aaagcaatac acagatagtc gaaagatttg aggacggtag
     2341 ttatatcgaa gtgacaatcg aagaaatatc aacgcttaca agaagtaaca ctaaaaaggg
     2401 taataaaaca acatactaca aaaacacata tgatgtaatc ttatggtctg tcaaaaccag
     2461 tggaacattt acttatgatg gctcaaccgc aaaatgtact gctgcatcag ttgacactca
     2521 atgtccagct ataaattgga aattgagtaa tattagatca tcaaaatctg gtgcaagcgc
     2581 ttatggatac gcaatcgcaa aaagttataa cggtcttgga atggttttac aaactataaa
     2641 cgaaacagta aaacttactt gcagtccaag tggaacatta tcttaaaaca attaaaaata
     2701 ggtataacat cgaaaattaa tctttttgaa tgttatacct atttttttgc actattcagt
     2761 taattcagtt tcaattatcc cgtgccagca ggtgtgattt ttcggagcgg ttctttcggg
     2821 tttgacaact ccaagcatct gataatcata tttgcttgta tcggcagcac catctatgta
     2881 atcagcttca actctgtata ctgggtacga gtaatctttc attcccttta aatcatctaa
     2941 tatatcttgc tgaatatctt caaacacatc atctatgtcc tcagccattt cgactcctat
     3001 taactcatgt gatttagtat ttttccaaat acacttaccg catctaaata ctagatattt
     3061 attcattttt ctttttctcc cattcagctt gtatttcttt ttcagccgca tctaaggtta
     3121 gttcccctat gtcgtatata gtcatattca taatcatatt gataatcatt gcttggcaac
     3181 aatatccatt gctacttttg caagatatag atttgttcct attcccgctg tggctgttat
     3241 tcctg
//
LOCUS       DAOMTA010000278         3241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1153_length_3241_cov_2.281230, whole genome shotgun sequence.
ACCESSION   DAOMTA010000278 DAOMTA010000000
VERSION     DAOMTA010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1153_length_3241_cov_2.281230"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /gene="pglX"
                     /locus_tag="PLK68_08245"
     CDS             complement(<1..2341)
                     /gene="pglX"
                     /locus_tag="PLK68_08245"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HRM90432.1"
                     /translation="MNKNPIKNYAIWARNELITRVTQKAFEYGVSKENIVDFNAESVN
                     GKLLTVEEKQQRQKLISEVKEKGFDQVMEEVAYTWFNRFIALRYMEVNNYLPNRVRVF
                     TNENNEFKPQLLDEAATIELDGLDKEKVYELLEANKNEELYKTLLLATCNDMHQYLPG
                     MFETIHDYKVLLFPENLLRNESVLGRLIFDIDEDSWLDQVQIIGWMYQYYNSELKDIV
                     MKKKSYSKEDIPAATQLFTPDWIVRYLVENSLGKLWIDGHPESETERWKYYIKEPTQV
                     DEVTSELKKLALRYKNINPEEITLIDPCMGSGHILVYAFEVFMDIYLECGYSSRDAAK
                     NIIEHNIYGAEIDSRAYQLAYFALMMKARAYDRRILYKQIKHNLVEIKDSKTIDINTL
                     HYLMDYKDIAVNLISIFSDAKELGSLIKVDIDKNELIKLKDYCRELERKINDYNLIER
                     LEIDELLEKINPILDEAICLAGMYDVVVTNPPYMAPTPVQKPYVNRNYPNSKSDLFAV
                     FIERCHEMNVLNGYQSMIVMPSLLFLSSFSKLREMLLDNVTINSLLHMGRGIFGIDFG
                     STAFTLANNKVKNYKGQYYRLHERTFQYIDPEHIKYLFLEAKRDSNLRYDFKIYDTEN
                     IPVLSKKEGNKISFVMNQNDFKLIPGVPLAYWTNDGILSSFIKGIPLGNLVIARNGMK
                     TGKNDEFTRLWFEVDYNKEMLNALNLDEAIQSGKKWYPYNKGGDARKWYGNNDYVVNW
                     ENRGDKIFNKAKEDRRNVQNYPDEMKFSPCVTWSLVSSGE"
     gene            complement(2355..>3241)
                     /locus_tag="PLK68_08250"
     CDS             complement(2355..>3241)
                     /locus_tag="PLK68_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRM90433.1"
                     /translation="GDEDGLIEYIITNFTKEQQTLNDYYNKNATRHYPGYNTIQEALK
                     LVAEVLNCKTDNIALVNKVCSLEDDLLDGKDDMVNVKNFFTTQKTLFDTATDYMQDVM
                     REKDYFTGHDEVETALQLIKNIVTYSDNYNYSKIKDLNGAIATIKDVYAELIKSKKKE
                     LEDIVEQCFNSVKDKANEDTSKLSGLLNQARQRFETRKEEISRTNSLALLDSKKMIIF
                     GDQDNYIQMMDKALKPVILNPKPVQPVVKKNTREFHRNVLFQTQTITSKEDIDKYVNA
                     IKARLLSYLEDCDEIKIK"
BASE COUNT          999 a          571 c          374 g         1297 t
ORIGIN      
        1 gctcaccaga tgagactaac gaccaggtaa cacatggaga aaatttcatt tcatctggat
       61 aattttgaac atttcttcta tcctcttttg ctttattaaa aattttatct cccctatttt
      121 cccaattaac aacataatcg ttatttccat accattttct agcgtcgcca cctttgttat
      181 atggatacca tttctttcca gactgaattg cttcatctaa atttaaggca ttcaacattt
      241 ctttgttata atctacttca aaccatagtc tagtaaattc atcatttttc cctgttttca
      301 tgccatttct agctattacc aaatttccaa gtggaatacc ttttataaaa gatgataata
      361 ttccatcatt tgtccagtat gccaatggta ctccaggaat aagcttaaaa tcattttgat
      421 tcataacaaa tgatattttg tttccctctt ttttagacaa gacaggtata ttttctgtat
      481 cataaatttt aaaatcatat cttaaattac tatctctctt cgcttctaaa aacaaatatt
      541 tgatatgttc tggatcaata tactggaaag ttcgctcatg taatctataa tactgtccct
      601 tataattttt tactttatta tttgcaagtg taaatgctgt cgaaccaaaa tcgataccaa
      661 aaatacctct tcccatatgt agtaaactgt taatcgtaac gttatccaac aacatttctc
      721 taagtttaga aaatgaggat aaaaatagta aagaaggcat tactatcatt gattgatatc
      781 cattcaatac attcatttca tgacaacgtt caataaaaac tgcaaataaa tcactttttg
      841 aatttggata gtttctattt acataaggct tctgaactgg tgttggagcc atatatggag
      901 gattggttac tacaacatca tacatacctg ccaaacaaat tgcttcatct agaatcggat
      961 ttattttttc taataattca tctatttcta atcgttcaat taaattataa tcattaattt
     1021 ttctctcaag ttccctacaa taatctttta gttttattaa ctcattttta tcaatatcaa
     1081 ctttaatcaa tgaacctaat tctttagcgt cactaaatat tgatattaaa tttaccgcta
     1141 tatctttata atccattaaa tagtgtaatg tattgatatc aattgtttta ctatctttga
     1201 tttctaccaa attatgttta atttgtttat atagaatcct tctatcataa gcccttgcct
     1261 tcatcataag tgcaaagtat gctaattgat acgcacggct atctatttca gcaccatata
     1321 tgttgtgttc tataatattt tttgcagcat ctcttgatga atatccgcat tctaggtaaa
     1381 tatccatgaa aacttcaaaa gcgtatacca aaatatgtcc tgagcccata catggatcaa
     1441 ttaaagttat ttcttcagga ttaatatttt tatagcgcaa agctaatttt ttcaattcac
     1501 tagtaacttc atcaacttga gttggctcct taatataata tttccatctt tcagtctcag
     1561 actctggatg cccgtcaatc cataattttc ctaaagaatt ttccactaaa tatctgacta
     1621 tccagtcagg cgtaaaaagt tgtgttgctg caggaatatc ttcttttgaa taggattttt
     1681 tcttcattac aatatccttt aattccgaat tgtaatattg atacatccat cctatgattt
     1741 gtacttgatc taaccagcta tcttcatcaa tatcaaaaat aagtcttcct aataccgact
     1801 cattccttaa taggttttca ggaaataaaa gtaccttata atcatgaatt gtttcaaaca
     1861 taccaggtag gtattgatgc atatcattac aagttgctaa caataaagtt ttatataatt
     1921 cctcgttttt attcgcttct aataattcat ataccttttc tttatctaaa ccatctaatt
     1981 caatagttgc agcttcatct aatagttgag gtttaaattc attgttttca ttagtgaata
     2041 cacgtactct gtttggaaga taattattaa cttccatata acgaagtgca atgaagcggt
     2101 tgaaccaagt atatgcaact tcttccataa cttgatcaaa acctttttct tttacttcac
     2161 taatcaattt ttgtctttgt tgtttttctt ctacagttaa caattttccg ttaacacttt
     2221 cagcattaaa gtcaacgata ttttctttac taacgccata ttcaaaagct ttttgggtta
     2281 ctctagtgat taattcatta cgcgcccata tagcatagtt cttaattgga tttttattca
     2341 tctaggtaac ctccttattt aatctttatt tcatcgcaat cttctagata tgataataat
     2401 cttgctttga ttgcatttac atacttatct atatcttctt tagaagtgat tgtttgtgtt
     2461 tggaataata catttctatg gaattctctt gtatttttct taacaaccgg ttgtactggt
     2521 tttggattta aaattactgg tttcaacgcc ttatccatca tttgaatata attatcttga
     2581 tcaccaaaaa taatcatctt tttagaatct aataaagcta aactatttgt tctagagatt
     2641 tcttcttttc ttgtttcaaa tctttggcga gcttgattta ataatcctga taatttagaa
     2701 gtatcttcat ttgctttatc cttaactgag ttaaaacatt gttcaacaat atcttctaat
     2761 tcttttttct tgcttttaat taattctgca taaacatctt taattgtcgc aatagcacca
     2821 ttaagatctt taatttttga ataattataa ttatcagagt atgtaacaat attcttgatt
     2881 agctgtaatg ctgtttctac ttcatcatgt cctgtgaaat aatctttttc tctcataaca
     2941 tcttgcatat agtcagttgc tgtatcgaat aatgtctttt gagttgtaaa gaagttttta
     3001 acattaacca tatcatcttt gccatctaat aaatcatctt ctaaactgca tactttatta
     3061 actaaagcaa tgttatccgt cttacaattt aaaacttcag caactagttt taatgcttct
     3121 tggattgtgt tatatcctgg ataatgtcta gtagcatttt tattatagta atcatttaat
     3181 gtttgttgtt ctttagtaaa gttagtgatg atatattcaa ttaatccatc ttcgtcacct
     3241 g
//
LOCUS       DAOMTA010000279         3240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1154_length_3240_cov_2.242072, whole genome shotgun sequence.
ACCESSION   DAOMTA010000279 DAOMTA010000000
VERSION     DAOMTA010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1154_length_3240_cov_2.242072"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1026
                     /gene="ligA"
                     /locus_tag="PLK68_08255"
     CDS             <1..>1026
                     /gene="ligA"
                     /locus_tag="PLK68_08255"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRM90434.1"
                     /translation="KLGATAKVPRWAVAYKYPPEEKETKLLAIELSVGRTGRITPTAI
                     FEPIRLCGTTVSRATLHNQDFIDDLDVRIGDTIVVYKSGEIIPKVKGVVKEKRPADST
                     PYVIGNTCPVCGAPAVREGDNADIKCTNHSCPSKLVRNIVNFVGRDAMDIKGFGFAYV
                     ETLVDHGYLKDLSDIYGLIDKRQELLDKKIIGLVKSTDNLLNAIEGSKNNDAIKLLTS
                     LGISNVGKSAAKSLMKKFKSIDNLMKASYAQLIEVNDIGDISAMAIINYFKNPDNQAV
                     VQRLKEYGVNMNIIEAQDGDERFDGKTFVVTGTLPTLSRKAASELIEKHGGKVSGSVS
                     KKTDYLLA"
     assembly_gap    1027..1036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1037..1126
                     /locus_tag="PLK68_08260"
     CDS             <1037..1126
                     /locus_tag="PLK68_08260"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90435.1"
                     /translation="GENAGSKLTKAQNLGINVISEETLLEMVK"
     gene            1171..2244
                     /locus_tag="PLK68_08265"
     CDS             1171..2244
                     /locus_tag="PLK68_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HRM90436.1"
                     /translation="MKKIVALLAIALVLSGCSDAKETVENQTANDVSTTDSLDDSFYR
                     VVNFNTNLSREDYYTAFGNTMDFQTIGRELQILSTDHFSTNDYYMSEGQYLKTDDMNQ
                     LLKRSEDTSKYPYTLQAQRGTTIGGVANPIMVSTVHEQDYYEKNGSEYVLKGLSLAIV
                     LDPRDEKNERLDTSLDESLVVDFGREAISKLYKYLQSKKDLKDIPANICVYYATNTNE
                     SDINGRYILKSYCDGSVGNIETLDYKNYMFTSTEATAADEDTASQFEIFKSNMKKAAT
                     EAVGVIGYGRYKNGQIQSMKINLKVNIKTYTELLYLISTAADELNTQFSGFDIKVLVS
                     SQDQLEALIVKDKGEDAKSILLY"
     gene            2255..2545
                     /gene="gatC"
                     /locus_tag="PLK68_08270"
     CDS             2255..2545
                     /gene="gatC"
                     /locus_tag="PLK68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRM90437.1"
                     /translation="MENKEEMLKKLGLKTMFNITDAEMPELVEEYEIFMNHVAVLEKI
                     DTDGKAPLAYPYEIETCFLRDDEPIDVISREDVLKNAKSVQDNQIKVPKVVG"
     gene            2545..>3240
                     /locus_tag="PLK68_08275"
     CDS             2545..>3240
                     /locus_tag="PLK68_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRM90438.1"
                     /translation="MKVYSIEELHNLIVNQEIDLNEYYYDLFKEVEFQQNRLNAFVTI
                     TKTQAMDNIKELKISDEEILKGIPGVFKDNYNTKGIKTTASSKMLEDYVPVYDAAVVE
                     KLYNEGVSLIGKSSMDELAMGGTNKSALTGPVYNPWDSSRIAGGSSGGSAALVGSGVV
                     PFALGSDTGDSIRKPAGFCGVVGFKPTWGRISRFGVIPYASSLDHVGAFTRNVRDMAI
                     VTEALAGRDERDMT"
BASE COUNT         1155 a          389 c          682 g         1004 t
ORIGIN      
        1 aaaactcgga gcaacagcta aagtacctcg ttgggcagta gcttataaat atccgcctga
       61 agaaaaagaa acaaaattat tagcaatcga attgtcagta ggtagaactg gaagaataac
      121 tccaacagcc atttttgaac caattcgttt atgtggaacg actgtatctc gagcaacttt
      181 acataatcag gattttatcg atgatctaga tgtgcggatt ggtgatacga tcgttgttta
      241 taaatctggt gagataattc ccaaggttaa aggagtcgtt aaagaaaaac gccccgctga
      301 tagtactcca tatgtaattg gcaatacttg tccagtctgt ggagctccag ctgtacgtga
      361 aggtgataat gcggatataa aatgcacgaa tcatagctgt ccaagtaagt tagttagaaa
      421 tattgttaac tttgttggac gtgatgctat ggatattaaa ggttttggct ttgcttatgt
      481 agaaacatta gtggatcatg gttatttaaa agatttaagt gatatttatg gtttaattga
      541 taaacgtcag gaattacttg ataaaaaaat tattggttta gttaaaagta cagataattt
      601 attaaatgca attgaaggta gtaaaaataa tgatgcaatt aaattgctaa cgagtttggg
      661 gattagtaat gtgggaaaat ctgccgctaa aagtttaatg aaaaaattta aaagtattga
      721 taatttgatg aaagctagtt atgctcagtt gattgaagtc aatgatattg gtgatattag
      781 tgctatggca attataaatt actttaagaa tcctgataat caggcagttg ttcaacgttt
      841 aaaagagtat ggagttaata tgaatattat cgaagctcaa gatggcgatg aacgttttga
      901 tggcaaaaca tttgtcgtaa cagggacatt accaactctt tcaaggaaag ccgcaagtga
      961 attgattgag aagcatggcg gtaaagtctc aggttcggtg tctaaaaaaa cggattactt
     1021 attagcnnnn nnnnnnggtg aaaatgcggg aagtaaactt accaaggccc aaaatttggg
     1081 aattaatgta attagtgaag aaactttact agagatggtg aagtagctaa aatagtgtta
     1141 taatgcactg tataaaggag gaacactaga atgaaaaaaa tagtggcttt attagcgata
     1201 gcattggtgc tgagcggttg tagtgatgct aaagagaccg ttgaaaatca aacagcaaat
     1261 gatgtatcca ctaccgatag tttagatgat tcattttatc gagtagttaa ttttaatacg
     1321 aatttaagtc gagaagatta ttatactgct tttggaaata ctatggattt tcaaacaatt
     1381 ggacgagaat tacagatttt atctactgat catttctcaa ctaatgatta ttatatgtct
     1441 gaagggcaat atcttaaaac ggatgatatg aatcaattat taaaacgctc agaggatact
     1501 agtaaatatc cttatacctt acaagcacag cggggaacta ctattggcgg agttgcaaat
     1561 ccaatcatgg taagtacagt gcatgaacaa gattattatg aaaaaaacgg atctgaatat
     1621 gtcttaaagg gtctatcatt agcaatcgta ctagatcctc gtgatgaaaa aaatgaacgt
     1681 ttagacacct cattagatga atcgttagtt gttgattttg ggcgtgaagc aattagtaaa
     1741 ttatacaaat atttacagag taaaaaagac ttaaaagata ttcctgctaa tatttgtgtt
     1801 tattatgcga ctaatactaa cgaaagtgat attaatggtc gttatatttt aaaaagttat
     1861 tgtgatggca gtgtgggaaa tattgaaaca ttagattaca aaaattatat gtttacaagt
     1921 acagaagcaa ctgctgctga tgaagataca gcatcacaat ttgaaatttt taaaagtaat
     1981 atgaaaaaag ctgcaactga ggcagttggt gtaattggct atggtcgtta taaaaatggt
     2041 cagattcagt caatgaaaat aaatttaaaa gtaaatatta aaacatatac tgaattgctt
     2101 tatttgattt ctactgcagc tgatgaatta aatactcaat ttagtggttt tgatattaaa
     2161 gtattagtta gttcacaaga tcaactagag gcattgattg ttaaagataa aggtgaagat
     2221 gcgaagagta tcttgttgta ttaggaggta atttatggaa aataaagaag aaatgttaaa
     2281 aaagcttgga ttgaaaacaa tgtttaatat tactgatgca gaaatgccag agttagtaga
     2341 agaatatgag atttttatga atcacgttgc cgtattagag aaaatagaca ctgatggtaa
     2401 ggcaccgtta gcatatcctt atgaaattga gacatgcttt ttacgtgatg atgaaccgat
     2461 tgatgtaata agccgagaag atgttttaaa aaatgctaag agtgttcaag ataatcaaat
     2521 taaagtacca aaggtggtgg gataatgaaa gtttattcaa ttgaagaatt gcataattta
     2581 atcgttaatc aagaaataga tttaaatgaa tattattatg atttatttaa agaagtggaa
     2641 tttcaacaaa atcgcttaaa tgcatttgtt acaatcacta aaactcaagc aatggataat
     2701 attaaagaac ttaaaatatc tgatgaagaa atattgaaag ggattcctgg agtatttaaa
     2761 gataattaca atactaaagg aattaaaacg acagcttcaa gtaaaatgct agaggattat
     2821 gtaccagttt acgatgctgc ggttgttgaa aaattatata atgaaggtgt tagtttaatt
     2881 ggtaaatcaa gtatggatga gttagcaatg ggtggtacta acaaatccgc tctaactggg
     2941 cctgtatata atccgtggga ttcatcaagg atagcgggag gatcatcagg tggcagtgca
     3001 gcacttgttg gtagtggggt tgttccattt gccttaggta gtgataccgg agattcaatt
     3061 cgtaaaccgg ctggattctg tggtgtagtt ggtttcaagc caacttgggg aagaatttca
     3121 agatttggtg ttattcctta tgcttcaagt ttagatcacg taggtgcttt tactcgaaat
     3181 gttcgtgata tggcaattgt aactgaagca ttagctggtc gtgatgaaag agatatgact
//
LOCUS       DAOMTA010000280         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1155_length_3235_cov_2.173585, whole genome shotgun sequence.
ACCESSION   DAOMTA010000280 DAOMTA010000000
VERSION     DAOMTA010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1155_length_3235_cov_2.173585"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLK68_08280"
     CDS             <1..1218
                     /locus_tag="PLK68_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90439.1"
                     /translation="HLFLGIVTLICSYYVYKNRENEYIGEPLVFRRIIIVLKAILIIA
                     VTICGFGVILLVSKINITYGFKGQSLLFLIYILIGMIVAIIVEIIFKGRHVYRNLLIY
                     VPVLAIVFGANFMIANKQYLSGINNSRYEVTADLVAELAGNQEYLSLSLNNEVTKEFL
                     NYLSKHRDDLHFEKYDDEQQDLAMMYTYQYEHGLDDDYYSYSIYYINKKAVIDFFNGP
                     GNKYFDKLLNYTEGLKKEKSLICYVNDYAVYLNKEEIQELIKLLPNQEIKPKNIFSIK
                     TYNLLNPDSVNYLISSNEAITNFITSDELIERAVFIENCRDVIDDINSDSTKFEEQIK
                     AVVSTQFSHETIDMVYPNGDSTVIAFSNDEVIYQGTYEVVTKEGNNYTYPLEFTLKKG
                     DDGVIVNDIKVGG"
     gene            1221..1556
                     /locus_tag="PLK68_08285"
     CDS             1221..1556
                     /locus_tag="PLK68_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90440.1"
                     /translation="MMISLLKNDLKQLKNTVVIFSGFLALSLVISLISFHSDVPVGMF
                     YLIILLYSLVVPMVNLSYLFDQTQQTHFSSLPYTKIQSFMIRYLSGLLCIIIPVIIYC
                     IVDVILGQD"
     gene            1586..2776
                     /locus_tag="PLK68_08290"
     CDS             1586..2776
                     /locus_tag="PLK68_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90441.1"
                     /translation="MIWIYYSLGNLAAYLTTSVIMDIILQIVINISPFIIYMSLFSVY
                     QTFVRGIISADFSMEVVSIILPAFRLFIGGLSGLSSYYLLLYAGYGLVIFIMAFLASS
                     NRECVNNYHGFTYKIIGEVIKLICIISVSWLITSILDIPVQSLKSFIIINIIATFVAT
                     FIIQFIQSKRIRYVLCIVQGTLIVLVTIVIFIGSKGYLENYIPHDIKAVEINSNFGYN
                     KINSKLTDQKSIDTVVAIHQEILKQKSDYGSHNLKITYYKDNGDKVTRGYQISDQEYL
                     MVIKKLDAALLKSWFDNYYYIINKLDTYQYLTYALPGESENEIRTENDILLFKNILQR
                     KLSEFENNPNLLLKVDYDNPGQITRFKRSNDGSESYQGDSISFDDNDPMSLALQEYDK
                     IKAN"
     gene            2823..3128
                     /gene="trxA"
                     /locus_tag="PLK68_08295"
     CDS             2823..3128
                     /gene="trxA"
                     /locus_tag="PLK68_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM90442.1"
                     /translation="MKILNSNEFDNAIASGIVLVDFYADWCGPCKMLSPVIEGLAEKM
                     EQVNFYKLNVDASSDIAGRYGVQAIPNLIIFKDGKAVDQITGFVPENEIVKHLQNLL"
BASE COUNT         1111 a          338 c          552 g         1234 t
ORIGIN      
        1 catttatttt taggaattgt taccttaatc tgcagctatt atgtttataa aaatcgtgaa
       61 aatgaatata ttggagaacc tttagtattt cgccgaatta ttattgtttt aaaagctatt
      121 ttgattattg ctgtaacgat atgtggtttt ggagtcattt tattagtatc aaaaattaat
      181 atcacatatg gattcaaagg tcagtcactt ttatttttga tctatatttt gattgggatg
      241 attgtagcga taatcgttga aattattttt aaaggaagac atgtttatcg taatttatta
      301 atctatgtac ctgttttagc aattgttttt ggtgctaatt ttatgattgc caataagcaa
      361 tatctttcag gaattaataa ttcgagatat gaagttactg ctgatttagt agctgaatta
      421 gctggtaatc aggagtatct tagcttaagc ttaaataatg aggttacaaa ggaattcttg
      481 aattatttga gtaaacatcg tgatgatctt cattttgaaa aatatgatga tgaacaacaa
      541 gatttagcaa tgatgtatac ttatcaatat gaacatggat tagatgatga ttattatagc
      601 tatagtatct attatattaa taaaaaagca gtaattgatt tctttaatgg accaggaaat
      661 aagtattttg ataagctgct taattatact gagggtttaa aaaaggaaaa aagtttaatt
      721 tgttatgtta atgattatgc agtctattta aataaagagg aaattcaaga attgattaaa
      781 ttgttgccaa atcaagaaat caaacctaaa aatatattta gtattaaaac atataattta
      841 ttaaatcctg atagtgtcaa ttatttaata agcagtaatg aagcaataac gaattttatt
      901 actagcgatg agttgatcga acgagctgtt tttattgaaa attgtcgaga tgttattgat
      961 gatattaata gtgattcaac aaagtttgaa gaacaaatca aagccgtagt gagtacacaa
     1021 tttagtcacg aaacgattga tatggtctat ccaaacggtg atagcacagt tatagccttt
     1081 agtaatgatg aagttattta tcaaggtacc tatgaagttg ttacaaaaga aggaaataac
     1141 tatacttatc cattagaatt taccttaaag aaaggtgatg atggcgtgat cgttaatgat
     1201 ataaaggtag gtggttaaag atgatgatca gtttattaaa aaatgattta aaacagctaa
     1261 aaaatacagt tgttattttt agtggttttc tagctttaag tttggtaatc agtctgattt
     1321 cttttcactc tgatgttcct gtaggaatgt tttatctaat tatcttactc tattcattag
     1381 ttgttccgat ggttaattta agttacttat ttgatcaaac ccaacagact catttttcat
     1441 ctttgccata tacaaaaata caaagtttta tgatcagata tttaagcgga ttattgtgca
     1501 taattatacc tgtcataatc tattgtattg ttgatgttat tttaggacag gattagtact
     1561 taaaaattgt tcttcagcaa ttttaatgat ttggatttat tatagtttag gtaatctagc
     1621 tgcctattta acgacatcag taattatgga tattattcta caaattgtta ttaatatatc
     1681 gccattcatt atttatatga gtttattttc tgtttatcag acttttgtta gaggaatcat
     1741 tagtgctgat ttttcaatgg aggtcgtaag tattattttg cccgcttttc gattatttat
     1801 tggtggactt agtggtttaa gttcttatta tcttttgctg tatgctggtt atggtctagt
     1861 tatatttata atggcttttt tagcaagcag taatcgggag tgtgttaaca attatcatgg
     1921 tttcacttat aaaatcattg gtgaagtaat taaactaatt tgtattattt cagtttcttg
     1981 gctgattact tctattttgg atattcctgt tcaatctttg aagagtttta ttattatcaa
     2041 tattattgca acctttgttg cgacttttat tattcaattt attcagtcaa aacgtattag
     2101 gtatgtattg tgcatagtgc agggaacatt aattgtttta gttacaattg taatttttat
     2161 tggttcaaaa ggatatttag aaaattatat tcctcatgat attaaagcag tagaaattaa
     2221 ttcaaatttt gggtacaaca agattaatag taaattaact gatcaaaaat caattgatac
     2281 agtagtggca attcatcagg aaattttaaa acaaaaaagt gattatggca gtcataactt
     2341 aaaaattact tattataagg ataatggtga taaggtaaca agaggttatc aaatttcaga
     2401 tcaagaatat ttaatggtaa tcaaaaagtt agatgcggca cttttaaaaa gttggtttga
     2461 taattattat tatattatta ataagcttga cacatatcaa tatcttactt atgcattgcc
     2521 gggtgaaagt gagaatgaaa ttagaacaga aaatgatatt ctattattta aaaatattct
     2581 tcaaagaaag ctgtcggagt ttgaaaataa ccctaattta ttattaaaag ttgactatga
     2641 taatccgggt caaattacta gatttaagcg ttctaatgat ggtagtgaaa gttatcaagg
     2701 tgattcgatt tcttttgacg ataatgaccc aatgtcgctt gctttacaag aatatgataa
     2761 aattaaagca aattagttgt aattatttat agattaggca taatgttaat gaggtgataa
     2821 aaatgaaaat tttaaatagt aatgaatttg ataacgcaat tgctagcggt atcgttttag
     2881 tagattttta tgctgactgg tgtggaccat gtaaaatgtt atctccagtt attgaagggt
     2941 tagcagaaaa gatggagcag gttaattttt ataaattaaa tgttgatgct tctagtgata
     3001 ttgccgggcg ttatggggtt caggcaattc caaatttaat tatttttaaa gatggtaaag
     3061 cagttgacca gattactggt tttgttcctg aaaatgaaat tgtaaaacat ttacagaatt
     3121 tattataatg tatttaagcc atggtagtgt taccatggtt tttatatttt gaaagttttt
     3181 gaaaaattca gctaaataag tctaaatatg ataaaatcta tacatataga aatgt
//
LOCUS       DAOMTA010000281         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1163_length_3229_cov_2.888469, whole genome shotgun sequence.
ACCESSION   DAOMTA010000281 DAOMTA010000000
VERSION     DAOMTA010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_3229_cov_2.888469"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLK68_08300"
     CDS             complement(<1..757)
                     /locus_tag="PLK68_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90443.1"
                     /translation="MENELIKTKIENLIKETKQLKIDDVISYIKIKFPENEFSWENID
                     ERSDFYYDRRDIIHLTNQTDLIINYPGGDRFGDIFAEPYTDISFHNLKLINKYDDLED
                     KLYLFLNDKYNSENKISNYVNELSRNDNLSIEEIIMHLSTDMNIPNHILNQISNEQLQ
                     NIKVREAIVNCVENSAFHRHFDACDQYQYGQYGGEDVDEWRLSKSLQFMAIWENLPDK
                     VKENCNYELEKAWLQIPDTKNVLNEEIEQLQERI"
     gene            complement(772..1416)
                     /locus_tag="PLK68_08305"
     CDS             complement(772..1416)
                     /locus_tag="PLK68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRM90444.1"
                     /translation="MKYFAKVPWTKGNSVANTGRKSKNTEDVMIFSKGKPRSLKLNAK
                     KNIQTALNNGLDIKGMDSSQVKALLEENNLVVHYMSGTSSMLPTQFNYQPKSITKRVY
                     QTEKPVELLEEIISYISLPYEVILDQFAGSGNLAVAALNVSRNAIVIEGVENIQNTSQ
                     EKRNLAKENFNLMKKNIEEQLDKKATIIDINPITYSNESTVGYEKLENDYDLEL"
     gene            complement(1413..1940)
                     /locus_tag="PLK68_08310"
     CDS             complement(1413..1940)
                     /locus_tag="PLK68_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90445.1"
                     /translation="MGLEESSMKKATIQMNLFDYFYEQDNFTIKEATELVKEVRGKRV
                     NDESIRARIYEGIDRGIFKRVSRGVYKVESQINGTTNQCLLINGDGRDLSMIKDNSID
                     GIITDHPYKLPKQLKGGNRNFAEYELFKYEEKDFKEKSRVLKDGAFIVEFLPEESEIN
                     YEYLYEIKQMAKKAG"
     gene            complement(2112..2816)
                     /locus_tag="PLK68_08315"
     CDS             complement(2112..2816)
                     /locus_tag="PLK68_08315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRM90446.1"
                     /translation="MISKKKANYMETNYTMIKGFSILCKALIIYILFYFIIGAVLISV
                     VPDDLSVYLRLTVTCITFIGMIFISKDYFKNKFVNDSMDVMDNSLKLAIISFSLVMVN
                     SFLTHINESMITASTYNNDLIKNIYTDSPVILGIISIALLPFIEEIFFKYVLFKNTDC
                     LKKHWIIKTLIIGLLFASVHCASEVIKLDITAFICMFNYFIFYVATNIIYHKKENLMM
                     VVIIHMLINICAVVLM"
BASE COUNT         1040 a          550 c          344 g         1295 t
ORIGIN      
        1 caattctttc ttgtaactgt tctatttctt cattcagtac attttttgta tcaggaatct
       61 gcaaccaagc tttttcaagt tcataattgc aattttcttt caccttatca ggcaagtttt
      121 cccagatagc catgaattga agtgatttag ataatctcca ttcatctaca tcttcacctc
      181 catattgccc atactgatat tgatcacatg catcaaaatg tctatgaaat gcactatttt
      241 caacacaatt gactattgcc tctcttactt taatattttg taactgttca tttgatattt
      301 gatttaaaat atgattagga atattcatat ctgtacttaa atgcataatt atttcttcga
      361 ttgaaagatt gtcattacgt gacaattcat tcacatagtt ggatatttta ttctcgctgt
      421 tatatttatc gtttaaaaat aaatataatt tatcttccaa atcatcatat ttattaatca
      481 actttaaatt atgaaaactt atatcagtat atggttcagc gaatatatca ccaaatctgt
      541 cacccccagg ataattaata atcaaatcag tttgatttgt taaatgaata atatctcgac
      601 gatcataata aaaatcactt ctttcatcaa tattttccca actgaattca ttttcaggaa
      661 atttaatttt tatatatgat attacatcgt caattttaag ttgttttgtt tctttgataa
      721 gattttcaat tttagtttta attagttcat tttccataac ttttctccaa attataattc
      781 taaatcataa tcattttcta atttctcata tcctacagta ctttcattgg aatatgtaat
      841 aggatttata tcaattatag tagctttttt atccagctgt tcttctatat tttttttcat
      901 tagattaaaa ttttcttttg ctaaatttct tttttcctgt gaggtatttt gtatattttc
      961 aactccttca ataacgattg catttctgga cacattcaat gcagctacag ctaaattacc
     1021 gcttccagca aactgatcaa gaataacttc atatggaagc gatatataac tgattatttc
     1081 ctctagcaac tcgactggtt tttcagtctg atatactctt ttagttatac ttttcggctg
     1141 atagttaaac tgcgtaggaa gcatacttga tgttccgctc atgtaatgaa caactaaatt
     1201 attttcctct aataacgctt tgacctgaga tgaatccatt ccttttatat ccagaccatt
     1261 atttaatgca gtttgaatgt ttttctttgc attaagtttt aatgatctgg gttttccctt
     1321 tgaaaaaatc ataacatctt cagtgttttt tgattttctt ccagtattag cgacagaatt
     1381 tccctttgtc catggtactt tcgcaaaata tttcaacccg ctttttttgc catttgtttt
     1441 atttcgtaca gatactcata gttgatttct gattcctcgg gtaaaaattc aactataaaa
     1501 gcaccgtctt tcagtactcg acttttctct ttaaaatctt tttcctcata tttaaaaagt
     1561 tcatattctg caaaatttcg attaccgcct tttaactgct taggcagttt ataaggatga
     1621 tcagtgatta tcccatcaat agaattatct tttatcattg ataaatctct gccgtcacca
     1681 ttaattaaaa gacattgatt tgttgttccg tttatttggc tctccacctt ataaacgcct
     1741 cgagaaaccc ttttgaagat tcctctatct atgccttcat atattctcgc cctaatactt
     1801 tcatcattta ctctttttcc tctaacttct tttaccagtt cagtagcttc ttttatggta
     1861 aaattgtcct gttcataaaa atagtcaaac aaattcattt gaattgttgc ttttttcatt
     1921 gaagattctt ctaaacccat tgattccacc tactttcctt aataatttct taaaattaaa
     1981 gacaaaaaag aggcattctg cctctttgca taattttaac tatactaatt ttagcataac
     2041 ctttgtttca aaaaggcaca atttttttca aaaaagtgaa cattttttga atattattca
     2101 actcttttac tttacattaa aacaacagcg catatattta tcagcatgtg tattattact
     2161 accatcatca gattttcttt tttatgataa ataatattag tagcaacata gaaaatgaaa
     2221 taattaaaca tacagataaa tgctgttata tcaagcttaa taacttcgct tgcacaatgt
     2281 acagaagcaa atagcaaccc tatgatcaaa gtcttaataa tccagtgttt ttttaaacaa
     2341 tcagtatttt taaaaagcac gtatttaaaa aatatttctt caataaatgg caaaagtgca
     2401 atacttataa tacccaggat aacagggctg tctgtatata tatttttaat taaatcatta
     2461 ttgtatgttg atgcagttat cattgattca ttaatatgag ttagaaaact attaaccatt
     2521 acaagagaaa atgaaataat tgctagtttt aaactgttat ccataacatc catagaatca
     2581 ttcacaaact tatttttaaa ataatctttt gaaataaaaa tcattcctat aaaagttata
     2641 catgtgactg tcaatcgaag atatactgat aaatcatctg gtacaacact aattaaaact
     2701 gctccaatta taaagtaaaa cagtatgtat ataattagcg ctttgcataa aatactaaat
     2761 ccttttatca ttgtataatt tgtctccata taatttgctt tcttttttga aatcatgaac
     2821 atgtctcctt tttgtaattt tataatttaa tttatatata tttataataa atatataaaa
     2881 agcatagcat atctgtttta caaaaaaggc acaatttttt ttactatatt tctcacaaaa
     2941 aaagaggcaa actgcctctt ttttatgatt ttatcctttt ttcatattct ctttctaata
     3001 ttttaatacg aaataatacc tttactaacc aaaatattgt taatcttcca cctatttcct
     3061 tgtccagtat tgcatcattt acatacatca cttattgcat catataaagc atctattttt
     3121 ttaggagtta aaacaaatat tcctttattt ttccagaggt taaatgaata atagtcgccc
     3181 atgtggggtt tcaagttttt taaaataatc tataaaatct tcgtctttc
//
LOCUS       DAOMTA010000282         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1165_length_3229_cov_2.277253, whole genome shotgun sequence.
ACCESSION   DAOMTA010000282 DAOMTA010000000
VERSION     DAOMTA010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_3229_cov_2.277253"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..511
                     /locus_tag="PLK68_08320"
     CDS             137..511
                     /locus_tag="PLK68_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HRM90447.1"
                     /translation="MCKVLMPIKPEYAEQILAGTKTFEYRKTRFKRNNVDAIVIYVTS
                     PVMKVLGEVELLDTLEDSPKNIWRKTSHNGGINKRLFDKYFEGKNKAIAYVLGGVKRY
                     SEEKTLKDFNINYYPQSFVYLD"
     gene            794..1750
                     /locus_tag="PLK68_08325"
     CDS             794..1750
                     /locus_tag="PLK68_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HRM90448.1"
                     /translation="MYIIIKAFLDDLIDFCYKGTVEPDMLAKYHKFYLKLTNKELKSK
                     LGHYIFSKKAIHLFNVKSSNNVACIVTLIHELSHHIDYMNRGTSDHSKDFYNVHIELI
                     MTLFKMGTVSYNEFINLKDYASNSNKLKRLVEKGYCVTEQLDYEKEKNWLIVKNCYEA
                     YAYLKEKNYVYNFYIKSWMKKININDIEKSKAEILNTSKNATFITRSGCELIFNLGDE
                     SKENNIPFKKTSESNITDVTKKCPKCGVKLVERTGKYGKFTACGNYPKCKYIMKDKKD
                     EIRETGELCPQCGSKLIERNGRYGKFIACSSYPKCKYVKKDM"
     gene            1821..2423
                     /locus_tag="PLK68_08330"
     CDS             1821..2423
                     /locus_tag="PLK68_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90449.1"
                     /translation="MKIVGVRLKKGIHKPIKDTAKIYYFMCPVKNVGIGDYVLLECDG
                     TDKINIFQVGLIIEEHDNTPENTELYMPFSFVVSGFPVKDFLARCDKVAEMRKRQIKK
                     IDEIIASDPIKYKKRVPKKKPRKKSIKNRLYALTVKCRDNELSEEEKIKVASELLKIY
                     YILMDNKTPREKRSSWMSSVMGCDVDEAKRYIELVRKHPK"
     assembly_gap    2697..2747
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2748..>3229
                     /locus_tag="PLK68_08335"
     CDS             <2748..>3229
                     /locus_tag="PLK68_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM90450.1"
                     /translation="RVSTMKQARDGIGIDTQKRMIAEHALATNLIKSTDELSFFIDDG
                     FSGGDLCRPKMNELLKEIKNGNVEMILAYDLARISRDVQDSNLFLKLILNHGIKLICL
                     YDDAKINTASDRFSTNIKLSNNQFERERTIERTNDGLISIVESGRYPCGGKMTFGWYR
                     "
BASE COUNT         1314 a          325 c          592 g          947 t
ORIGIN      
        1 aggtattata aagggtgctc cacaatctat acatacatta gatcatgata aatttaaaaa
       61 aatagtgaaa ataagtggaa gtcaaaggca aattaaaata taatgtaaag atggtcgtat
      121 aaggaggaat tttgatatgt gtaaagtact aatgcctatt aaaccagaat atgcggaaca
      181 aattctagca ggtacaaaaa catttgaata tagaaagact agatttaaaa ggaataatgt
      241 agatgcgatt gttatttatg taacatctcc tgtaatgaaa gttttaggtg aagtagaact
      301 attagatact ttagaagatt ctcccaaaaa tatttggaga aaaacttcac ataatggagg
      361 aattaacaag cgtttatttg acaaatattt tgaaggaaaa aataaagcta tagcttatgt
      421 tttaggcggt gtaaaaagat atagtgaaga aaaaacactt aaggatttta acattaatta
      481 ttatccacag tcctttgtgt atttagatta gaagttgata agtaagtaaa gtaaaaggaa
      541 agtctaaata ataaattaga aggagtcaat aaagagatat tacagtcttt agatgagggc
      601 gtgttgatta tatcccgata gttttagaac aaaaaggaac ttttctagtt ttgttatcca
      661 aaatttcata ttcagtataa actaagcagt tagtcatatt tgatttactg cttttatttt
      721 tgatagtaga aaatgtttag taataaaaga aagaacaaaa ttataataaa tgcgtattag
      781 aaggagaatg cagatgtata taataataaa agctttttta gatgatttaa ttgatttttg
      841 ctacaaagga acagtcgaac ccgatatgtt ggcaaaatac cataaatttt atttaaaatt
      901 aactaataag gaattaaaaa gcaaattagg acactacata ttttcaaaaa aagctataca
      961 tttatttaat gttaaaagta gtaacaacgt tgcctgtata gtaacgctaa tacatgaact
     1021 aagtcatcat attgattata tgaacagagg tacatctgat catagtaaag atttttacaa
     1081 tgttcatatt gagcttatta tgacgttatt taaaatggga acagtcagct ataatgaatt
     1141 tattaactta aaggattatg cgagtaattc taataaatta aaacgtttag tagaaaaggg
     1201 atactgtgta acggaacagc tagattatga aaaagaaaaa aactggctaa tagttaaaaa
     1261 ctgttatgaa gcatatgcgt acctaaaaga aaagaactat gtttacaatt tttatattaa
     1321 gtcctggatg aaaaaaatta atataaatga tattgaaaaa tcaaaagctg aaattttgaa
     1381 tacaagcaag aatgcaacgt ttattacaag atctggatgt gaactgattt ttaatttggg
     1441 cgatgaaagt aaagaaaata atataccttt taaaaagaca tctgaatcta acattacaga
     1501 cgtaactaaa aagtgtccta agtgtggtgt aaaactagtg gaaagaacgg gtaaatatgg
     1561 taaatttact gcttgtggca attatcctaa atgcaaatat attatgaaag acaaaaagga
     1621 tgaaattagg gaaactgggg aactatgtcc gcaatgtggc agtaagttaa tagagagaaa
     1681 cggaagatac ggcaagttta ttgcatgcag cagctatcca aaatgcaagt atgttaagaa
     1741 ggatatgtaa aataaattgt gccttttatt aaaacaaaat atgctatagt gatcattata
     1801 attagattgg agagaaaaga atgaagattg taggggtaag attaaaaaaa ggaattcaca
     1861 aaccgattaa agatactgca aaaatttatt attttatgtg tcctgtaaaa aatgtgggta
     1921 taggagatta tgttcttttg gaatgtgatg gcactgataa aataaatata tttcaagtgg
     1981 ggctaataat tgaagaacat gataatactc ctgaaaatac agagctgtat atgccttttt
     2041 cgtttgttgt ttcaggattc cctgtaaaag attttttggc cagatgtgat aaagttgcag
     2101 aaatgcgaaa aagacaaata aaaaaaatag atgagataat tgcatcagat cctataaaat
     2161 ataaaaaaag agtgcctaaa aagaaaccca ggaaaaaaag tataaaaaac agattatatg
     2221 cactaactgt aaaatgtcga gataacgaac ttagtgagga agaaaaaata aaagttgcaa
     2281 gtgaactttt gaaaatctat tacattctta tggataataa aactccaaga gaaaagagat
     2341 cgtcttggat gagcagtgtc atgggatgtg atgttgatga agcaaaaagg tatatagaat
     2401 tagttagaaa acacccaaaa taaacttttg ataatgaaag tactgatgaa aaattggaag
     2461 tacagataag gaaaatcata gtaaaaaaat agaattagac ccagaaaaaa gagattaaac
     2521 tttattattt aatctctttt attttggatt attattaagt agaaaataat aaaaggtata
     2581 tagaattttt tgattaacta gtgtaaattt atttactgga tagttaagga ttttttcatt
     2641 aactatcctc ttggcataat gaagaaaaga gggagaaaga aatgaaatta aaaattnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta gagtatcgac
     2761 tatgaaacaa gcgcgtgacg gtattgggat tgatacacaa aaaagaatga ttgccgaaca
     2821 tgctttagca acaaatctta taaaaagtac tgacgagtta agtttcttta tcgatgatgg
     2881 tttttcaggt ggtgaccttt gcaggccgaa aatgaatgaa ctattaaaag aaataaaaaa
     2941 tgggaatgtt gaaatgattt tagcatacga tcttgcaaga atatccagag atgtgcagga
     3001 cagtaattta tttttaaagt tgatattaaa tcatggtatt aaacttattt gtctatatga
     3061 tgatgcaaaa ataaatactg caagcgacag atttagtaca aatataaaac tgtcaaacaa
     3121 tcagtttgaa agagagagga caatagagcg aacaaatgac gggttgatca gcatagtgga
     3181 aagcggtaga tatccatgtg gcggtaaaat gacatttgga tggtacagg
//
LOCUS       DAOMTA010000283         3208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1185_length_3208_cov_2.485886, whole genome shotgun sequence.
ACCESSION   DAOMTA010000283 DAOMTA010000000
VERSION     DAOMTA010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1185_length_3208_cov_2.485886"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLK68_08340"
     CDS             <1..208
                     /locus_tag="PLK68_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HRM90451.1"
                     /translation="NHLNDFVTKRAMTLKTKVMLLSFASTMLLIAFLMMDNIYGRITI
                     VLLVIFKYYYFAFRIKTIKEVSND"
     gene            201..1937
                     /locus_tag="PLK68_08345"
     CDS             201..1937
                     /locus_tag="PLK68_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRM90452.1"
                     /translation="MINKRLVSYLKEDKKYIYLQVLMQWLALIGQVVIVAIIANMINE
                     LYQGHQVNNFSIKVIIIIVLILIKGYFGKLVSSFSFKAAKNIKGKLRCDIYQKVLLLN
                     NHYSDVISTASLTQLMSEGVEQLEIYFGKYLPQFFYSMLAPLTLFIILGRVEFKAALV
                     LFVCVPLIPVSIVLVQKFAKRLLNKYWGLYGNLAERFLDNVRGLTTLKGYQGDQAKHL
                     EMNEEAQRFRNITMKVLVMQLNSISIMDLVAYGGAALGIIISLYSYQAGAIDLGQTFM
                     MIMLSAEFFIPLRLLGSFFHIAMNGNAASEKIFRLLDTPVNDHKELEITEIKKIEITR
                     LSFGYDEEIVLKDVSLEIDEPGIYGVVGSSGSGKSTIAKLLMGYYDNYQGVLSYNGNQ
                     VNQVKHQSLMKQITMVEHNPYIFAGTVRSNLSDGNDNCDDSIMIEVLKKVNLWNYFDG
                     VNGLDSEIEERGNNLSGGQKQRLSIARALLHDTSVYIFDEATSNIDIESEEIIMKVIE
                     QMRDEKIVILISHRLASVENCKCNYVFSQGRLIGWGNHKELMRNNPEYIELVNTQKEI
                     ENYGGQANAKTE"
     gene            1921..>3208
                     /locus_tag="PLK68_08350"
     CDS             1921..>3208
                     /locus_tag="PLK68_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90453.1"
                     /translation="MQKRSNLKVVISLIALVKSMLLIMLGAIILGVIGYLAAIFLTVI
                     AGEIMIKVIKHETFTSLLYLLIACGILRGFLRYGEQWCNHFIAFKLLAMIRDIVFVKL
                     RKLAPAKLDGKDQGNLVAMITSDVELLEVFYAHTISPVMIALIVSIIMIIYIGYYHFA
                     LGLLASTAYLFIGVIIPLIVGKKSGNMGLNYRNDYGKYTSYVLESVHGLRIIDQFSRG
                     NKRLKEIDKKSELLNEQNKELKDLEGQYRMIGDICVSLFSLAMFILGYYLYRQGSIEF
                     NGVLISTIALMASFGPVLALSSLAHNLVITFASGNRVLDLLAEEPQVETVINGYQGNL
                     GTIEINNLSFKYEAETILDKINLSIKPGEIIGIEGRSGSGKTTLLKLLMRFYDPTDGE
                     ILINGHDIKTWQSDSLAKLISYVTQDTYVFNDSIINN"
BASE COUNT         1107 a          346 c          630 g         1125 t
ORIGIN      
        1 aaatcattta aatgactttg taactaaaag agctatgaca ttgaaaacca aagtgatgtt
       61 gttatcgttt gcaagtacga tgttattaat tgcattttta atgatggata atatttatgg
      121 aaggataaca attgttttat tagtaatatt taagtattat tattttgcat ttcgaattaa
      181 aacaattaaa gaagtgagca atgattaata aaagattagt tagttattta aaagaggata
      241 aaaaatatat ttatttacaa gttttgatgc agtggctggc attgattggt caagtagtaa
      301 ttgttgcaat tatagctaat atgatcaatg aactttatca ggggcatcaa gtaaataatt
      361 ttagtattaa agtcataatc ataattgtat taattttgat taaaggttat tttggtaaat
      421 tagtatcatc tttttctttt aaagctgcaa aaaatattaa aggaaaacta agatgtgata
      481 tttatcagaa agtattatta ttaaataatc attatagtga tgtaatcagt acggcttcat
      541 taacgcaatt aatgagcgaa ggagttgaac aattagagat ttattttggt aaatatttgc
      601 cacagttttt ctatagtatg ttagcaccac tgacactgtt cattattttg ggacgagtag
      661 aattcaaagc agccctagta ttgtttgttt gtgttccttt aattccggta tcgatcgtct
      721 tagttcaaaa gtttgctaaa agattattaa ataagtattg gggtctttac gggaatcttg
      781 ctgaacgctt tttagataat gttcgcggct tgacaacatt gaaagggtat caaggggatc
      841 aagctaaaca tcttgaaatg aatgaagaag cacaacgatt tagaaatatt acgatgaagg
      901 ttttggtaat gcagctaaat tctatcagca ttatggatct tgttgcttat ggtggtgcag
      961 cactaggaat tattatttca ctatacagtt atcaagctgg agcaattgat ttagggcaaa
     1021 catttatgat gatcatgtta tcagcagagt ttttcattcc gttaagactg ctaggatcat
     1081 ttttccatat cgctatgaat ggtaatgccg caagtgagaa aatttttagg ttattagata
     1141 caccggtaaa tgatcataaa gagttagaaa ttactgagat taaaaaaatt gaaattacta
     1201 gactgtcgtt tggatatgat gaagaaatag tattgaagga tgttagttta gaaattgacg
     1261 aacctggtat ttatggtgtg gttggaagct cagggagtgg taaaagtacg attgctaaat
     1321 tattaatggg ttattatgat aattatcaag gtgttttaag ctacaatgga aatcaagtca
     1381 atcaagtcaa acatcaaagt ttaatgaaac aaataacaat ggttgaacat aatccttata
     1441 tttttgcagg aactgtccgt agtaacttaa gtgatgggaa tgataactgt gatgatagta
     1501 taatgattga ggtattaaag aaagttaatt tatggaatta ttttgatgga gttaatggtt
     1561 tggatagtga aattgaagag cgtggaaata atttgtctgg tggacaaaaa caacgtttaa
     1621 gtattgcccg agcattattg catgatacta gtgtttatat ttttgatgaa gcgacgtcta
     1681 atattgatat tgaaagtgaa gaaataatta tgaaagtaat tgagcagatg cgtgatgaaa
     1741 aaattgttat tttaatttca catcgtttag caagtgtcga aaattgtaaa tgtaactatg
     1801 tctttagtca agggagatta attggctggg gaaatcataa agaattgatg agaaataatc
     1861 cagaatatat agagttagtt aatacccaaa aagaaattga gaattatgga ggccaggcaa
     1921 atgcaaaaac ggagtaattt aaaagtagta atcagtttga ttgctttagt caaatcaatg
     1981 ctcttaatca tgcttggagc aattatttta ggtgttattg gctacttggc tgctattttt
     2041 ctaacggtaa tagcaggaga aatcatgatt aaagtaatta agcatgagac ttttactagt
     2101 ttattatatt tattgattgc ttgtggaatt ttaaggggct ttctacgtta tggggaacaa
     2161 tggtgtaatc attttatcgc atttaaatta ttagcgatga tccgagatat tgtttttgtg
     2221 aaacttagaa aattagcacc agcaaaactt gatggtaaag atcaaggaaa cttggtagcc
     2281 atgattacta gcgatgtaga acttttagaa gtgttttatg cacatacaat ttccccagtg
     2341 atgattgctt taattgtttc gattattatg attatctata ttggatatta tcattttgct
     2401 ttaggtcttt tagcaagcac tgcttaccta tttattggtg taattattcc tttaattgtt
     2461 ggtaagaagt cgggaaatat ggggttaaat tatcggaatg attatggtaa gtatactagt
     2521 tatgtactag agtctgtcca tggtctaaga attattgatc aattttcccg tggtaataaa
     2581 cgcctaaaag aaatagataa aaaaagtgag ttattaaatg agcaaaataa agaattaaaa
     2641 gatcttgagg gtcaatatcg aatgattgga gatatttgtg ttagtttatt ttcactggca
     2701 atgtttatct taggatatta tttatatcgc caaggatcaa ttgaatttaa tggcgtttta
     2761 attagtacga ttgctttaat ggcatcattt ggaccagtct tggctttatc ttcattagca
     2821 cataatctag ttattacttt tgcaagtgga aatcgtgttc ttgacttatt agctgaagaa
     2881 cctcaagttg aaactgtaat caatggatat caggggaatt taggaacgat tgaaataaat
     2941 aatttatcat tcaaatatga agctgaaacg attttagata aaatcaattt aagtattaaa
     3001 cctggtgaaa tcattggaat tgaaggacga agtggaagtg gtaaaacaac gctattgaaa
     3061 ttattgatgc gtttctatga tcctactgac ggagaaattt tgattaatgg tcatgacatc
     3121 aaaacatggc aaagtgattc tttagcgaaa ttaattagtt atgttaccca agatacatat
     3181 gtttttaatg atagtattat caataata
//
LOCUS       DAOMTA010000284         3207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1186_length_3207_cov_2.450825, whole genome shotgun sequence.
ACCESSION   DAOMTA010000284 DAOMTA010000000
VERSION     DAOMTA010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_3207_cov_2.450825"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLK68_08355"
     CDS             complement(<1..307)
                     /locus_tag="PLK68_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRM90454.1"
                     /translation="MIILLELTSRQFLIIRLLQKNTVPLSANTLSRLLDISPRTLRND
                     IFQINKNIEDFHISSSKNGYQLTLLSENAHMLINDIKISEQSKSTNIILQYLLNHPSC
                     "
     gene            445..2439
                     /locus_tag="PLK68_08360"
     CDS             445..2439
                     /locus_tag="PLK68_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM90455.1"
                     /translation="MNQYYPYLFQPLRVNTMMLKNRIIASTMGIPKSHELLSTTHYGN
                     VSIIDKSVGGAAMTFVSIESAANANGEFPKHDRDGIRESISVARQYGAKVGTWCVPRL
                     KQDAANDIRAHYDGKQVLAPSPYLTRSGARAVELTLEDIQGIMKQARNDALAIKRFGF
                     DFIYLYVGYEELTTQFLSPVFNKRTDEYGGSLENRMRFTIEHVKTIRNAVGTDFPIVI
                     LLGASDYLKGSYQFEEMLELLKRIEDDVDLINVSAGMDMIPGYFPEEQIIEPELGLEA
                     WYSVNGKHCQSIFEPHLTNVHWAKLVKQNFPNKLVGVIGSIMTPKEAEQLLQEGVVDI
                     ISMGRPLVADPFLPRKAMQGQSDDIVPCIRCLQCYHSATEHTNVQCSVNPRYRREHRV
                     PLKLEKSEIIKKVIVVGGGPAGCKAAITAHDRGHQVILIEQRDKLGGQLNLAQHEMHK
                     QELKAYRDYLENQINKRDIQVMYKTTATKNLLEQLNGDVVLVAIGASPIRLHFPGEQL
                     EYVDYFENVYPKLDSLKDNVCIVGGGQVGIELAVELLERGKLVTVIEMTNQIASQGHI
                     LYRAGLRRLLKRFEKQLTILINSQCLGFSESGVKIINKNGESIIKCDNAIIAVGMKPN
                     REEAFKLYGIADETMMFGDCEKFGQVVGATNDAYFIAANI"
     gene            2452..>3207
                     /locus_tag="PLK68_08365"
     CDS             2452..>3207
                     /locus_tag="PLK68_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90456.1"
                     /translation="MKKLQDKLIEVGNKLATQRHLQSISFGLMAIMPLTIFGSIFQLI
                     SALPDIFPFIPSYSETIKDAILFPYNMVFGLFGVIAVCAIAYYHARTYKLDLLQAPIV
                     SLLSFIVLAAPIVDNNMDATYLGSQGIFLAIFVALVTVEIMQLLKRRNIQIKLPDSVP
                     PAVAASFDAIIPMCLIVAGFYGLSLLCQNFTGMLLPSWIMSLLTPAIQGSESLWFCML
                     MAFIIAFLQFLGVHGFNVVSGIILPLLIANTGAN"
BASE COUNT         1070 a          438 c          657 g         1042 t
ORIGIN      
        1 gacatgatgg gtgattaagc agatattgta aaattatatt ggtagacttt gattgttcgg
       61 atattttaat atcatttata agcatatggg cattttcgct taataaagtt aattgatagc
      121 catttttgga tgaagaaata tgaaaatcct caatattttt attaatttga aaaatatcgt
      181 ttctaagtgt tctaggacta atatctagta atcttgatag cgtattagca cttagtggaa
      241 cggtattttt ttgtaataga cgaataatta aaaattgtct agaggtaagt tcaagcaaaa
      301 taatcactcc tttatcatat tataacaaac ttcttgatag aaatcttaaa attgtgattc
      361 agcttttgcc atgatcgtgg caaaagctgg tttggatgca aaagctattt gacatacaat
      421 aagagagcaa ggaggagttg gaaaatgaat cagtattatc catatttatt tcaaccatta
      481 agagttaata caatgatgtt gaaaaataga attattgcat caacgatggg gattcccaag
      541 agccatgaat tattatctac gactcactat ggaaatgtta gtattataga taagtctgta
      601 ggtggagcag caatgacttt tgtatcaatt gaatctgcgg ctaatgccaa tggtgaattt
      661 ccaaaacatg atcgcgatgg tattcgtgaa tctatttcag tagcccgtca gtatggggct
      721 aaggttggaa cttggtgtgt accacgacta aaacaagatg cagctaatga tattcgagct
      781 cattatgatg gtaagcaggt tttagcacca tcaccatatt taacaagaag tggcgctcgt
      841 gcagttgaat taacgcttga agatattcag ggaattatga aacaggcaag aaatgatgca
      901 ttggcaatca aacgatttgg ttttgatttt atctatttgt acgttggata tgaagaatta
      961 acaacccagt ttttatctcc agtatttaat aaaagaacag atgaatatgg gggttcgcta
     1021 gaaaatcgta tgcgctttac aatagagcac gttaaaacga ttcgtaatgc tgttggcaca
     1081 gactttccaa ttgttatatt gttaggagcg agtgattatt taaaagggtc ttaccaattt
     1141 gaagaaatgt tggaattatt aaaaagaatc gaagatgatg ttgatttgat caatgtatca
     1201 gctgggatgg atatgattcc aggatatttt ccagaagagc aaataataga gcctgaatta
     1261 ggattggaag cttggtatag tgttaatggg aaacattgtc aaagtatttt tgaaccgcat
     1321 ctaactaatg ttcattgggc aaaattagtt aaacaaaact ttcctaataa actagtcggt
     1381 gtaattggtt caataatgac gccaaaagaa gctgagcaat tattacaaga aggtgtagtt
     1441 gatattattt caatgggtag acctctagtc gcagatccat ttttaccacg aaaggcaatg
     1501 caggggcaaa gcgatgatat tgtaccatgt attcgctgtt tacaatgcta tcatagtgct
     1561 acagaacata caaatgtaca atgctctgta aaccctcgat atcgtcggga acatcgggta
     1621 ccattaaagt tagaaaaaag tgagattatt aaaaaagtga ttgtcgttgg tggtggtcct
     1681 gctggttgta aagctgcaat aactgcacat gatcggggac atcaagttat tttgattgaa
     1741 caacgtgata aattgggggg acaattaaat ttggcccaac atgaaatgca taaacaggaa
     1801 ttaaaagcat atcgagatta tctagaaaac caaattaaca agcgagatat tcaagttatg
     1861 tataaaacga ccgctactaa aaatttatta gagcaactaa atggtgatgt ggtgcttgtt
     1921 gccattggag cttcaccaat tcgcctccat ttcccagggg aacaattaga atatgttgat
     1981 tattttgaaa acgtttatcc taaattggat tctttaaaag ataatgtttg tattgtcggc
     2041 ggaggacaag ttggaataga gttagcagta gagttattag aacgtggaaa attagtaaca
     2101 gtaatagaaa tgactaacca aatagctagt caagggcata ttttatatcg agcaggatta
     2161 aggcgtttgt taaaacgatt tgaaaaacag ttgaccattt taataaatag tcaatgctta
     2221 ggatttagtg aaagcggagt taaaataata aataaaaatg gcgaatctat tattaaatgt
     2281 gataatgcca taattgctgt tggaatgaaa ccaaatcgtg aggaagcttt taaattgtat
     2341 ggaatagctg atgaaacgat gatgtttggt gattgtgaaa agttcggaca agtagttgga
     2401 gcaactaatg atgcgtattt tattgcagca aatatatagg aggaaaataa aatgaaaaaa
     2461 ttacaagata aacttattga agtgggcaat aagttagcaa cacaaaggca tttgcaatcg
     2521 attagttttg gattaatggc aataatgcca ttaacgattt ttgggagtat tttccagctt
     2581 atttcagcat taccagatat atttccattc atacctagtt atagtgagac gataaaggat
     2641 gcaattttat tcccgtataa tatggttttc ggtttatttg gagtcattgc agtatgcgct
     2701 atcgcttatt accatgcacg aacatataaa cttgatttat tacaagcgcc gattgtatcg
     2761 ctgttatcat ttattgtttt ggcagcacca attgttgata ataatatgga tgcaacttat
     2821 ctaggatcac aaggaatctt tttagcaatt tttgttgctt tggtaaccgt ggaaatcatg
     2881 caattactaa agagacgtaa tattcaaatt aaattacctg atagtgttcc tccagcagta
     2941 gctgcttcat ttgatgcgat tattccgatg tgcttgatcg ttgctggttt ctatggatta
     3001 tctttacttt gtcaaaattt tacagggatg ctattaccat cttggataat gagtttatta
     3061 acaccggcga ttcaaggttc tgaatcatta tggttctgta tgttgatggc atttattatt
     3121 gcatttttac aattcttggg agtacatggg tttaatgttg tatctggaat aattcttccg
     3181 ttattgattg ccaatacagg tgctaat
//
LOCUS       DAOMTA010000285         3201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1189_length_3201_cov_2.303560, whole genome shotgun sequence.
ACCESSION   DAOMTA010000285 DAOMTA010000000
VERSION     DAOMTA010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_3201_cov_2.303560"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3201
                     /locus_tag="PLK68_08370"
     CDS             <1..>3201
                     /locus_tag="PLK68_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM90457.1"
                     /translation="PDDHDNFLNFWDLPKLHETFSSNQHQILKGYFRKKKTDGKYCWV
                     LQTVVAIENSQMNDEIIMCFIQEINEPPTFQQLPDKKQFNELTGLYQTNSFFQIADNL
                     LKTDTSHQYCMVAIDIEHFKLFNEWYGIAAGDLFLRAIAKELKTISQQLRGIAGYLLG
                     DDFALLIPYHNNHTKMILDKLMHYIEEFQDKIGFYPALGVCLVDDRNMPSQTIYDRAV
                     IALTSIKGNYAKRIAYYQDKMQLQMEEEHKLLLDFQNAIENNEFTFYAQPKCNIQTGK
                     IVGAEALVRWQHPKKGIIPPNEFIPILEKNGLIGKMDYYIWDCVYKHLRKWIDNGHKA
                     IPISVNVSRIDMFTLDVVKCFKDLVNKYQINHNLIEIEITESAYVEEYDKVKTIIKEL
                     RQEGFLVSMDDFGSGYSSLNMLKDINVDVLKIDMNFLNMNEQSSDKGIGILEAIINMA
                     KLMGLRVIAEGIESSQQVELLLDLGCLYAQGYYFYKPMTSKQFEAIIKNDDKVDYRGI
                     QAKQINYISIKTLVDHHVLSDAMLNNILGGIAFFEFDGHNIELLNVNQNYCKIVDESG
                     VDVEEKRLKILENIYEPDQTIFLDLFEQATVHLINGTKGQIRYLTAKGTYKWLNIYLF
                     LLKKLDHSNLYYAQVSDITEQKKKEKQLESSQKALAAAVHISENDDSFMNLAEENKAL
                     ASSIFSQMSPGGMIGGYCEDGFPLFFANDALVKLLGYETYEEFAIAIKEQVINTIHPD
                     DREQVAKDIGSNYYSGLEYTTTYRMPKKDGTWFWTLDKGKVIETEDGRLAIVSACIDI
                     SETIAAQQKLARHNETLQKVNQELYYLNNKLPGGYHRCADTPDYDFIHISNRFLEFFN
                     YTRQEIKELFNDKFINMIHPDDRAKAVRTTENLSQQDESFDLEYRMLAKDGYIWVIDQ
                     TSVLEYNGTRFFQGVVTNVTKNIELRNQMQLLEKYSPVDIVLITCRKNNVKHTIITNG
                     LISKYGYNKEQYQRYLDNKEFEYNFNRDAFKIFEQNIIKAFKQQTDYYEILSININSK
                     TVWFKTSFEFVKADAEEIQYLYISSDITAFKEKEYH"
BASE COUNT         1150 a          477 c          531 g         1043 t
ORIGIN      
        1 accccgatga tcatgataat tttttaaact tttgggatct tccaaaactt catgaaacgt
       61 ttagctctaa tcagcatcaa attctaaaag gctatttcag aaaaaagaaa acagatggta
      121 aatactgttg ggtcttacaa accgttgtag ctattgaaaa ttcccaaatg aatgatgaaa
      181 tcatcatgtg ttttatccaa gaaataaatg aacccccaac ttttcagcag cttcctgata
      241 aaaaacagtt caatgaacta actggacttt atcagacaaa ttcatttttt caaattgctg
      301 ataatttatt gaaaactgat acttcccatc aatattgtat ggtcgctatc gatattgaac
      361 atttcaaact atttaatgaa tggtatggaa ttgcagcagg tgatctattc ttacgtgcaa
      421 ttgctaaaga acttaaaaca atcagtcaac aattaagggg tattgccggg tatttattag
      481 gtgatgattt tgcactttta attccttatc ataataatca tacaaaaatg attcttgaca
      541 aattaatgca ctacattgaa gaatttcaag ataaaattgg gttttatcca gccttgggag
      601 tttgcctcgt tgatgataga aatatgcctt cccaaactat ttatgaccga gcagtcatcg
      661 cccttacatc aataaaagga aattatgcta aacgaattgc ctactatcaa gataaaatgc
      721 aattacaaat ggaggaagaa cataaactgc tccttgattt tcaaaatgct attgaaaaca
      781 acgaatttac tttttatgct cagccaaaat gtaatattca aactggaaaa attgttgggg
      841 ctgaagctct ggttcgctgg caacatccta aaaaaggaat tattcctcct aatgagttta
      901 ttccaattct tgaaaaaaat ggcctaattg gcaaaatgga ttattatatt tgggattgtg
      961 tgtataaaca tcttcgaaag tggatcgata atggacataa ggcaattcct atttccgtta
     1021 atgtttcacg aattgatatg tttactctcg atgtagttaa atgtttcaaa gatttagtaa
     1081 acaaatatca aattaatcat aatctaattg aaattgagat tactgaaagc gcctatgttg
     1141 aagaatatga taaagtcaaa acaataataa aagaattacg acaagaaggt tttttagttt
     1201 caatggacga ttttggcagt ggttattcat ccctcaatat gctcaaagat attaatgtcg
     1261 atgttttaaa aattgatatg aattttttaa acatgaatga gcaaagtagt gataaaggaa
     1321 ttggtatttt agaagcaatc atcaatatgg ctaaattaat ggggcttaga gtcattgctg
     1381 aaggaatcga gtccagccag caagttgaac ttcttttaga tttgggctgc ctatatgctc
     1441 aaggctatta cttttacaaa ccaatgacta gcaaacaatt tgaagcaatt attaaaaatg
     1501 acgataaagt tgactatcgt ggtatccagg caaagcaaat caattatatt agtattaaga
     1561 ccttagttga tcatcatgta ttgagcgatg ccatgttaaa taatatttta ggtggtattg
     1621 cattttttga atttgatggt cataatatag aacttttaaa cgttaatcaa aattattgta
     1681 aaattgttga tgaaagtggc gttgatgttg aagaaaagcg actaaaaata ttagaaaata
     1741 tttatgagcc tgatcaaact atttttctcg acctatttga acaagctaca gtccacctaa
     1801 tcaatggtac gaagggacaa atccgttact taactgctaa gggcacttat aagtggttaa
     1861 atatttattt atttttgtta aaaaaactag atcactcgaa tttgtattat gcgcaggttt
     1921 ctgatattac tgaacaaaag aaaaaagaaa aacagttaga atcttctcaa aaagctctag
     1981 ctgctgccgt tcatatttca gaaaacgatg attcttttat gaatttagct gaagaaaata
     2041 aagccttagc ttcctccatt ttttctcaaa tgtcacccgg aggcatgatt ggcggttatt
     2101 gtgaagatgg ctttccactt ttcttcgcta atgatgcctt agtcaaatta ctcggttatg
     2161 agacttatga agaattcgca atagctatca aggagcaggt tattaataca attcatcctg
     2221 atgatcgtga acaagtagct aaagatattg gttccaatta ctattctgga ttagaataca
     2281 ctacaactta tcgaatgcct aaaaaagatg gtacttggtt ctggacgctt gataaaggga
     2341 aagttattga aacagaagat ggacgattag cgattgtcag tgcctgtatt gatatttcag
     2401 aaaccattgc tgctcaacaa aaactagcta gacacaatga aacactacaa aaagtaaatc
     2461 aggagcttta ttatttgaat aataaattac ctggcggtta tcatcgttgt gccgatacac
     2521 ctgattatga ttttattcat attagtaatc gcttcttaga attttttaac tatacccggc
     2581 aagagataaa agaacttttt aatgataaat ttataaatat gattcatcct gatgatcgtg
     2641 ctaaagcagt tcgtacaact gaaaatttat ctcagcaaga tgaatcgttc gacttagaat
     2701 atcggatgct tgctaaagat ggatatattt gggtaattga ccagacatct gtattagaat
     2761 ataatggcac aagatttttt caaggggttg ttactaacgt tactaaaaat attgaattac
     2821 gtaatcaaat gcagctatta gagaaatact ctcctgttga tattgtttta ataacttgta
     2881 gaaaaaataa tgtaaaacat acaatcatta ctaatggtct aatttctaaa tatggttata
     2941 ataaagaaca atatcagcga tatcttgata ataaagaatt tgaatataac tttaatcgtg
     3001 atgcctttaa gatatttgaa caaaatatta ttaaagcctt caaacaacaa acagactatt
     3061 acgaaattct atcaattaat attaattcaa aaacagtttg gtttaaaact agttttgagt
     3121 ttgttaaagc tgatgcagaa gaaatccaat acctttatat ctctagcgat attaccgcat
     3181 ttaaagaaaa agagtatcat t
//
LOCUS       DAOMTA010000286         3191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1199_length_3191_cov_2.190689, whole genome shotgun sequence.
ACCESSION   DAOMTA010000286 DAOMTA010000000
VERSION     DAOMTA010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_3191_cov_2.190689"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2779)
                     /locus_tag="PLK68_08375"
     CDS             complement(<1..2779)
                     /locus_tag="PLK68_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM90458.1"
                     /translation="MYLKRIELHGFKSFADKVNVEFQPGITGIVGPNGCGKSNISDAV
                     RWVLGEQSVKSLRGANMSDVIFAGSEDRRAQNLAEVTLVFDNSDRFMKYDYNEVEITR
                     RLYRMNNEAEYLINKQSCRLKDIIDLIMDTGLGKDSLSIISQGNISSFADNKPEERRG
                     IFEDAAGVSKYKKRKLESIRKLERTNENLERIGDIVVELEKQVGPLKRQKDKAEKYLA
                     LKEKLTAIEVNVLINEITEAKKSLDELSKVIKDLNERQASLEADILLKESSNDEIKKK
                     MFTLDQEINALQSKLLEAVSNVSKLETAKVEVDQKRKHALETLSKENLKENIANMKAI
                     LSDVVNEYNDRVERLDNTQEELTTIIGQQEERNKKLLLLKNEIDQLSNNINKNKSRKE
                     ILIDAIENKSNYHHGIKTVLSLAKSNKNIIGVLGDLITTDEGYELALSSALAGAIEFI
                     VTTDDLTARDAIKFLRDNKAGRATFLPISAMKPRHVREEHLVVCQTMEVYLGLASDFV
                     TYEDEIAAVVLNQLGNIIVAKDIDSANEISKATFSRYKVVSLEGDVVNVGGSLTGGSF
                     NRQKSSIVQKRELEQVAVTLEQQEKELGLKRNEHNGLDNEIKEVSHALLQKQMAYAKL
                     EVVVQSKKEELIKAKSEYESLADQSVELEEFASGKTESKLVNQLNEAIKYRDNLTEEI
                     KSKRELRMAYVNQNEALDVELREYRNDLKEVQSEVNTSAINATKLEAMLNNHLARLND
                     EYRMTYEYAVEHYLDEIDIEQAKLEVYELRTNINRLGNVNVDAIEEYQIISERYENMN
                     TQRIDLIQAQDSILEAIKEMDEIMVERFSETFEKINEEFNHVFRSLFGGGKARIKYTD
                     PTNILETGIDIDVQPPGKAVQNITLFSGGEKALIAISCLFAILRVRPIPMCILDEVEA
                     ALDIANVERFAK"
     gene            complement(2796..>3191)
                     /locus_tag="PLK68_08380"
     CDS             complement(2796..>3191)
                     /locus_tag="PLK68_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRM90459.1"
                     /translation="SGIGIALATAFEFDDAPVVFAKKGTAKTTSTHYYSSKVHSFTKG
                     IDYDAIVSKKYLDKDDNILIIDDFLANGQAVLGLVDIVKQAGANIAGVGIVVEKGFQT
                     GRQLIEEKGYRVESLAVVEAFRDGAVILK"
BASE COUNT          970 a          661 c          384 g         1176 t
ORIGIN      
        1 atttagcaaa acgttcaaca tttgcaatat ctaatgcagc ttcaacctca tctaagatac
       61 acatcggaat tggccgtacc cgtaaaattg caaataaaca tgaaatagcg atcaaggcct
      121 tttccccacc tgaaaataat gtaatattct ggactgcttt acctgggggc tgaacatcaa
      181 tatcgattcc agtttcaaga atatttgtag gatcagtata ctttattcta gcctttcctc
      241 caccaaataa actcctaaag acatgattga actcttcgtt gattttctca aacgtttctg
      301 aaaaacgctc aaccataatt tcatccatct ctttaatcgc ttcaaggata ctatcttggg
      361 cttgaatcaa atctatacgc tgagtattca tgttctcata acgctcactg attatttgat
      421 attcttcaat tgcatcaaca ttaacgtttc ctaaacgatt aatatttgtt cttaattcgt
      481 atacttctaa tttagcttgt tcaatatcaa tttcatcaag gtaatgctcg acagcatact
      541 cataagtcat ccgatattca tcatttaatc tagctaaatg attatttaac attgcttcta
      601 attttgttgc attaatagca ctagtattaa cctcactttg aacctctttt aaatcattac
      661 gatattcacg caattcaaca tctaaagctt cattttgatt aacataggcc attctaagtt
      721 cacgttttga tttaatttct tcagttaaat tatcacgata tttaatcgct tcatttaact
      781 gattaactaa tttactttct gtttttccac tagcaaattc ttctaattca acactttgat
      841 cagctaatga ttcatactcg gattttgcct tgatcaactc ttctttcttg ctttgaacaa
      901 caacttccaa tttagcatat gccatctgct tttgtaatag agcatgtgaa acttctttga
      961 tttcattatc caatccatta tgttcattac gcttaagccc aagttctttt tcttgttgtt
     1021 caagagtaac agcaacctgc tcaagttcac gtttttgaac tattgaagat ttttgacgat
     1081 taaaacttcc acccgtcaaa gaaccgccaa cattaacaac atcaccttca agagaaacga
     1141 ctttgtaccg agaaaaagtt gctttgctga tttcattagc actatcaata tctttagcta
     1201 caataatatt ccctagttga tttaaaacaa cggcagcaat ctcatcttca tatgttacaa
     1261 aatcactagc caaacctaaa tatacttcca tggtttgaca aacaactaaa tgttcttcac
     1321 gcacatgtcg tggtttcata gcactgattg gtaaaaaagt agctcgtccc gctttattat
     1381 cacgtaaaaa tttaattgca tctcgtgcag ttaaatcatc tgttgtaaca ataaactcta
     1441 ttgctcctgc taaagctgaa gacaacgcta attcatatcc ttcatcagtt gtaattaaat
     1501 cacctaaaac accaataata tttttattac ttttagcaag acttaaaact gttttaatgc
     1561 catgatgata atttgattta ttttcaatcg catctattaa aatctctttt cgtgatttat
     1621 ttttattaat attgtttgat aattgatcaa tttcattttt taataaaagt aattttttat
     1681 tacgctcctc ttgttgtcca attatcgtgg ttaactcttc ttgagtgtta tctaatcgtt
     1741 ccacacgatc attatattca ttaacaacat cacttaaaat cgccttcata ttagcgatat
     1801 tttcttttaa attttcttta cttaaggtct caagtgcatg ttttcgcttt tgatcaactt
     1861 cgactttggc agtctctagt ttcgagacat ttgatactgc ctctaatagt ttactttgta
     1921 aagcatttat ttcttggtcg agagtaaaca ttttcttttt tatttcatcg ttactgctct
     1981 ctttaagcaa aatatcagcc tctaaagaag cttgacgctc atttaagtcc ttgattacct
     2041 tactcaattc atctagtgat ttttttgctt cagttatctc atttattaaa acattaactt
     2101 ctatagctgt taatttttct ttcaacgcta aatacttctc tgctttgtct ttttgacgtt
     2161 taagaggacc aacctgtttt tctagttcta ctacgatatc accaattcgt tcaagatttt
     2221 cgttagtacg ttctaattta cgaatcgatt ctaattttct ttttttatat ttagaaactc
     2281 ctgctgcatc ttcaaaaata ccacgacgtt cttctggttt attgtccgca aaagaagaaa
     2341 tattgccttg ggaaataatt gataacgaat cctttcctag acctgtatcc ataatcaaat
     2401 caataatatc ttttaaacga caagattgtt tattgattaa atactccgct tcattattca
     2461 tgcgataaag tcgccgtgta atctcgactt cattataatc atatttcata aaacgatcac
     2521 tgttatcgaa aactaaggta acttcagcta aattttgcgc ccgacgatct tcgcttccag
     2581 caaaaataac atctgacata tttgcaccac gcaacgattt aactgactgt tctcctaaaa
     2641 cccaacgtac agcatcagag atatttgatt tcccgcaacc atttggacct acgattcctg
     2701 tgattccagg ttgaaattct acattgactt tatcagcaaa tgatttaaaa ccatgtaatt
     2761 caattctttt taaatacatt actttcttcc tctcattatt tcaggatcac cgcaccatcc
     2821 ctaaatgctt ctactactgc taacgattcc acacgatagc ctttttcttc aattaattgg
     2881 cgacctgttt gaaaaccttt ttcaacaaca attccaacac cagcaatatt tgcacctgct
     2941 tgtttaacaa tatcaactaa acctaataca gcttgaccat tagctaaaaa atcgtcgata
     3001 attaagatat tatcgtcttt atctaaatat tttttactga caatcgcatc ataatcaatt
     3061 cctttagtaa atgaatgtac tttagaacta taataatgtg tacttgttgt ttttgcagtc
     3121 cctttctttg caaagacaac aggtgcatca tcaaattcaa aagctgttgc taaagcaatc
     3181 ccaattcccg a
//
LOCUS       DAOMTA010000287         3184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1200_length_3184_cov_2.094599, whole genome shotgun sequence.
ACCESSION   DAOMTA010000287 DAOMTA010000000
VERSION     DAOMTA010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_3184_cov_2.094599"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..934
                     /locus_tag="PLK68_08385"
     CDS             11..934
                     /locus_tag="PLK68_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM90460.1"
                     /translation="MKIATREAFGEALKELVIKNNDVIVLDADLSPATKTCYAKEARP
                     QQFYSTGIAEANMVGIGAGLAVQGLKPFVSSFAMFLAGRAFEQIRNSIAYPHLNVKLC
                     ATHAGLTVGEDGATHQCNEDIALMRVLPGMVVLQPCDANETKAMMQFMVEYDGPVYLR
                     TSRYAVDNILDSNYKFLLGEIVPIVKGCKIAFLATGIMVNEARKAIEILAKNNIYPSL
                     YNVSSLKPINTMQLNSIINSYDLIFTLEEHNIIGGLYSLVCEKLDKPKKIYPIAVQDT
                     FGESGTPEELMSKYKIDADYLVKSVLETVEE"
     gene            937..2305
                     /locus_tag="PLK68_08390"
                     /pseudo
     CDS             937..2305
                     /locus_tag="PLK68_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            2328..>3184
                     /locus_tag="PLK68_08395"
     CDS             2328..>3184
                     /locus_tag="PLK68_08395"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HRM90461.1"
                     /translation="MSRKCIIGQSGGPTVVINATLAGVIQGALKADYEKIYGMINGID
                     GLLDEKMVDLSSFKDQKNLFKLIHTPAMYLGSCRYKIFEKDQSIKQRIITILNKYEIT
                     DFFYIGGNDSMDTIEKLSTYAKICNCPINFIGIPKTIDNDLMNIDYTPGYPSACHYVA
                     TSLCEIAFDSVIYPIKSVTIVEIMGRDAGWLTASAQLVNEVYPDSIDLIYMPENIFDE
                     NDFLEQIKDKLKKKDHCIIAVSEGIRDQYGMCVSLDSGIQKDAFGHLSNNGCSNYLKK
                     LIGDHLGCKV"
BASE COUNT         1134 a          407 c          655 g          988 t
ORIGIN      
        1 ggtcattagg atgaagattg caacaagaga ggcctttgga gaagcactaa aagagctagt
       61 gataaaaaat aacgatgtta ttgttttgga tgcggattta tcgcccgcaa caaaaacatg
      121 ttatgctaag gaagcaagac cacagcaatt ttatagcaca ggaattgctg aagcaaatat
      181 ggtcggaata ggtgcaggtt tggcggttca ggggttaaag ccttttgtaa gttcatttgc
      241 catgttttta gcagggcgtg cttttgaaca aattaggaat tctattgctt acccacattt
      301 aaatgtaaaa ctttgtgcaa ctcatgccgg cttgactgtt ggcgaagatg gagcaactca
      361 tcaatgtaat gaagatattg ctttaatgcg agttttgccg ggcatggtag ttttgcaacc
      421 gtgcgacgca aatgaaacaa aagctatgat gcagtttatg gttgaatatg atggtcctgt
      481 atatttaaga acgagcagat atgcagtgga taatattttg gactctaatt ataaatttct
      541 tttaggcgaa atcgttccaa ttgttaaagg ttgtaaaatt gcttttttag cgactggtat
      601 tatggtaaat gaggctagga aagctattga aattttagct aaaaataata tatacccgtc
      661 attatacaat gtgtcctcac ttaaaccaat aaatacgatg caactaaatt caattataaa
      721 tagttatgat cttatcttta cattggaaga acacaatatt ataggtggtt tgtatagttt
      781 agtttgtgag aagttggata agcctaaaaa gatatatcca attgctgtcc aagatacatt
      841 tggtgaaagt ggaacaccag aggaattaat gagtaaatat aagattgatg cagattacct
      901 agtaaagtct gtattagaaa cagtggagga ataaaaatga caaagtttaa agatgatttt
      961 ttatggggtg gcagtttagc agctaatcaa tgcgaaggtg cttttgatgc agatggaaga
     1021 ggtctagatt taatggatgt aattccgggc ggattagaag caaggcaggc agcatatgct
     1081 tcaccacttg attatatgaa tgaaaatatt gagtattgtc caaatagaat tgcaatcgat
     1141 ttttaccatc gttacaaaga ggatattgct ttgtttggag agatgggttt caaatgttta
     1201 cgtttgtcag ttaactggac gcgtttattt ccgtatggag atgaagaaca accaaatgaa
     1261 aaaggcctaa catactatga tagtttaata gatgaattac ttaaacatgg aattcaacct
     1321 ttaataacac ttaatcattt taatgtaccg ttgtatttag caaagcatta tggtggctgg
     1381 gctaatcgaa aaatgatcga tttctactgc cgactagtcc gtctgttaat gacccgttat
     1441 aaaggaaagg taacaaaatg gttaactttt tgtgaaatca atgttggttt attttataga
     1501 aaaggtgata atattgaaca aattaagtat caggcaatgc atcatcagtt actcgcttca
     1561 gcattagcaa caaaaatagg acatgagatt gataaaaata atcaagttgg atgtatgatt
     1621 gcaggtgggg tcgtctatcc gtatacttgt cacccatcag atgtattaaa agcttttgag
     1681 gaaaataata aggaactaat gttgacagat gttcaagttt ttggagaata tccatatcat
     1741 atgaaagcta aaatgaaaaa agaaggaatc actatacata tggaaaaaga tgatgaggag
     1801 attctaaaaa ataatacagt tgactttgtt gctttcagtt attatgcaag taaagtagta
     1861 acgacagatc acagattaaa gatcaataac tatggcaagt tacaaaatcc ttattgtgat
     1921 gcatcacaat ggggatggat cattgattct cttacaagaa agatttcata aaccattatt
     1981 tattgttgaa aatggtttag gagcacctga tgttgtagaa acagatggct caattaaaga
     2041 tgattataga atagaatatt taaaagctca tattgaacaa gtaaaactag cactagatga
     2101 tggaattgat ttgatgggtt acttaacctg gggcccaata gatgtaattt cagcaactga
     2161 aggtcaaatg acaaaacgtt atgggtttat atatgttgat cgagatgatg aaggaaaggg
     2221 aacattaaaa agatcaaaga aaaaatcatt taactggtat aaaaaagtta ttcaatcaaa
     2281 tggtgaggaa ttagaaaata tttaaagcag ataaggggat gaagatcatg tcgagaaaat
     2341 gtataatcgg acaatctggt gggccgacag tagttattaa tgcaacatta gccggggtca
     2401 ttcaaggagc tttgaaagca gattatgaga aaatatatgg aatgattaat ggaatagatg
     2461 gattgctaga tgaaaagatg gttgatttaa gtagttttaa agatcagaag aatttgttta
     2521 agttgattca tacaccagca atgtatttag gctcatgtag atataagatt tttgaaaagg
     2581 atcaatcaat taagcaaaga ataattacaa tattaaataa gtatgaaatt actgacttct
     2641 tttatattgg tggtaatgat tcaatggata ctattgaaaa attaagtact tatgcaaaaa
     2701 tatgtaattg cccaatcaac tttatcggta ttcctaaaac aattgacaat gatcttatga
     2761 atattgatta tacaccagga tacccttctg cctgtcatta tgttgctacc tcattatgtg
     2821 aaattgcttt tgacagtgtt atatatccaa taaaatcagt aacgatcgta gaaattatgg
     2881 ggcgagatgc tggctggtta acagcttcag cacaacttgt taatgaagtc tatcctgatt
     2941 ctatcgattt aatttatatg ccggaaaaca tttttgatga aaatgacttt cttgaacaga
     3001 tcaaagataa attaaaaaag aaggatcact gtattattgc tgtatctgaa ggtattcgag
     3061 atcaatatgg aatgtgtgta tcactagata gtgggattca aaaagatgca tttggtcatt
     3121 tatcaaataa tggctgttca aattatctaa aaaaacttat cggtgaccat cttggctgta
     3181 aagt
//
LOCUS       DAOMTA010000288         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1215_length_3173_cov_2.963438, whole genome shotgun sequence.
ACCESSION   DAOMTA010000288 DAOMTA010000000
VERSION     DAOMTA010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_3173_cov_2.963438"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1842
                     /gene="kdpA"
                     /locus_tag="PLK68_08400"
     CDS             109..1842
                     /gene="kdpA"
                     /locus_tag="PLK68_08400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRM90462.1"
                     /translation="MDIFVKYLGYLIVLVVLAVPLGFYINKVMNGKKVFLSRILEPCE
                     NFIYRVLHVKKDEEMSWKKYLVSVLIFSGFGLVFVFLLQMLQGVLPGNPAKVEGTTWD
                     LALNTAISFVTNTNWQAYSGESGLSYLTQSLGLTVQNFVSAGTGLAVLYALIRGFTRI
                     KAKGLGNFWVDLTRSVIYVLMPLSVVLSIILVSQGVTQSIDEYQTVKLAEPIVLEDGT
                     EITEQTVPLGPAASQIAIKQLGTNGGGFYGVNSAHPLENPTGLSNLVEVVSILLIPAA
                     LCFSFGKGIKDSRQGIAIFVAMGIMLVAALGSIAVSEQMATPQLEQNGIVDISNHDQA
                     GGNMEGKESRFGIVGSSTWAAFTTAASNGSVNSMHDSYTPLGGMVTMLLMQLGEVIFG
                     GVGCGLYGMLGFVILAVFMAGLMVGRTPEYLGKKIEPYEMKWAVVVCLATPVAILVGS
                     GIASLVPQVADSLNNSGAHGFSELLYAYSSAGGNNGSAFAGFAANTPFINVTLGLVML
                     FVRFIPILGILAIAGSMVQKKKVAVTAGTLSTCSPLFVFLLVFVVLLVGALSFFPALA
                     LGPIAEFFGMF"
     gene            1858..>3173
                     /gene="kdpB"
                     /locus_tag="PLK68_08405"
     CDS             1858..>3173
                     /gene="kdpB"
                     /locus_tag="PLK68_08405"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRM90463.1"
                     /translation="MKTETKSALMDKQMVIRAIKDSFYKLAPKTQAKNPVMLLVYISA
                     ILTTALFVISLFGIKDANSGFTLGIAVILWFTVLFANFAEAIAEGRGKAQADSLRAAK
                     KDVEAYKIPSIDQKDIVEIVSSATLTKGDIVLVKAGQQIPGDGEVIDGVASVDESAIT
                     GESAPVIRESGGDRSAVTGGTTVLSDWIVVQISSEPGESFLDKMISMVEGANRKKTPN
                     EMALQIFLVALSIIFVLVTMALYAYSAFGAKQAGMDNPSSVTVLVALLVCLAPTTIGA
                     LLSAIGIAGMSRLNQANVLAMSGRAIEAAGDVDTLLLDKTGTITLGNRQASAFIPVDG
                     VTSEELADAAQMASLADETPEGRSIVVLAKEQFNLRGRSINQMHMEFVPFSAKTRMSG
                     VNYQGNEIRKGAADTIKKYVTENGHIYSDECEKVVTEIANLGETPLV"
BASE COUNT          974 a          447 c          720 g         1032 t
ORIGIN      
        1 gtgtaaaagg aggacactta tgaatgtaat cgtaggagtg attggggtaa tcggtgttgc
       61 aatattaggt taccttacag tgatattaat taagggggat aaacagtaat ggatattttt
      121 gtcaaatatc taggatatct aatcgttctt gttgttttag cagtaccgtt aggtttttac
      181 attaataaag tcatgaacgg taaaaaagtt tttttatcaa gaattttaga accatgtgaa
      241 aatttcattt atcgagtttt acatgtaaaa aaagatgaag agatgtcttg gaagaagtat
      301 ttagtaagtg tattaatatt tagtggattt ggattagtat ttgtcttttt attacaaatg
      361 ctgcaaggag ttttgccggg taatcctgca aaggttgaag gaacaacatg ggaccttgcg
      421 ttaaatacag caataagttt tgttactaac actaactggc aagcttatag tggtgaaagt
      481 ggattaagtt atttaacaca atcgttaggt ttaacggttc aaaactttgt ttctgcagga
      541 acaggactag cagttttata tgcattgatt agaggcttta caagaattaa ggctaaaggc
      601 ttaggaaatt tttgggttga tttaacaaga tctgtaatct atgtcttaat gccgctatca
      661 gttgtcttat caattatttt agtgagtcaa ggtgtaactc aaagtattga tgagtatcaa
      721 actgttaaat tggccgaacc aattgtttta gaagatggta ctgaaattac agaacagaca
      781 gtacctttag gacctgcggc cagtcagatt gcaatcaagc aattaggtac taatggtggt
      841 ggtttctatg gcgttaactc agcccatcca ttggaaaatc caactggatt aagtaattta
      901 gtagaagttg tatcaatatt attgattcct gcggctcttt gtttttcatt tggcaaggga
      961 attaaagata gtcgtcaagg tattgctatc tttgtcgcaa tgggaattat gctggtagca
     1021 gcacttggaa gtattgctgt tagtgagcag atggcaactc cacaattaga acaaaatgga
     1081 attgtcgata ttagtaacca tgaccaagct ggcggaaata tggaaggtaa agaatcgcgt
     1141 tttggaattg ttggatcatc aacttgggcc gcttttacaa cagcagcatc aaatggttca
     1201 gtcaattcaa tgcatgatag ttatacacca ttaggcggaa tggtaacaat gttattaatg
     1261 cagttaggtg aagtaatttt tggtggtgtt ggctgtggtt tatatggaat gttaggattt
     1321 gttattttag ctgtatttat ggctggatta atggttggac gaaccccaga atatttaggt
     1381 aagaagattg agccttatga gatgaaatgg gccgtagttg tttgtttagc aacacctgta
     1441 gctattcttg taggtagtgg gattgcttcc ctagttcctc aagttgcgga tagtttaaat
     1501 aacagcggtg cacacggttt ttcagaattg ttatatgctt attcttcagc aggtggtaat
     1561 aatggttcag cttttgctgg ttttgcagca aatacaccat ttattaacgt aacacttgga
     1621 ttggttatgt tatttgttag atttattcca attttaggta ttttggctat tgctggcagc
     1681 atggtacaaa agaaaaaagt ggctgttact gcgggtacat tatcaacttg ttcaccacta
     1741 tttgtctttt tattagtatt cgtagtgtta ttagttggag cactaagttt ctttccggct
     1801 ttagctctag gaccaattgc agaatttttt ggaatgtttt aatcggggga taaatatatg
     1861 aaaacagaaa caaagagtgc tttaatggat aaacaaatgg tcattcgtgc tattaaagat
     1921 tctttttata aattagcgcc taagacgcaa gcaaagaatc ccgtaatgtt attagtatat
     1981 atttcagcta ttttaacgac ggctttgttt gttatttcgt tatttgggat taaagatgca
     2041 aattctggat ttactctagg aatagcagta attttatggt ttacagtgct gtttgctaat
     2101 tttgcagaag cgattgctga aggacgtgga aaagcgcagg cagattcatt gcgagcagct
     2161 aagaaagatg ttgaagctta taagattcca agcattgatc aaaaagatat agttgaaatt
     2221 gtatcatcag caacgctaac aaaaggtgat atcgtacttg ttaaagcagg acaacaaatt
     2281 cctggtgatg gtgaagttat tgatggtgta gcttcagttg atgaaagtgc aataactgga
     2341 gagtcagcac cggttatccg tgaaagcggt ggtgatcgaa gtgcagtaac cggtgggact
     2401 acagttttat cagattggat cgttgttcaa atttcaagtg aacctggaga gagtttctta
     2461 gataagatga tttccatggt tgaaggtgcc aaccgaaaaa aaacaccaaa cgaaatggcc
     2521 ttacaaattt tccttgtagc cctttcaatt atctttgttt tagtaactat ggcactttac
     2581 gcgtattcag cttttggagc taaacaagca gggatggata atccttcttc ggtgacagta
     2641 ttagtagctt tactagtatg tttagcacca acaacaatcg gagcactgtt atcagcaatt
     2701 gggatagctg gaatgtctcg attaaatcaa gctaatgtac tcgccatgag tggtcgggca
     2761 attgaagcag ccggtgatgt tgatacctta ttacttgata aaacaggaac aatcacactg
     2821 ggtaatcgtc aagctagtgc atttattccc gtagatgggg taacgagcga agagttggct
     2881 gatgctgctc aaatggcttc attagctgac gaaactcctg aaggacgtag tattgttgtt
     2941 cttgcaaaag aacaatttaa tcttcgggga cgaagtatta atcaaatgca tatggaattt
     3001 gtaccattca gtgcaaaaac gagaatgagt ggtgttaatt atcaaggtaa tgaaattcgt
     3061 aaaggtgcag ctgatacaat taaaaaatat gtaacagaaa acggacatat atatagtgat
     3121 gagtgtgaaa aggtagttac tgagattgct aatcttggag aaacaccatt agt
//
LOCUS       DAOMTA010000289         3168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1220_length_3168_cov_2.730806, whole genome shotgun sequence.
ACCESSION   DAOMTA010000289 DAOMTA010000000
VERSION     DAOMTA010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_3168_cov_2.730806"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PLK68_08410"
     CDS             <1..140
                     /locus_tag="PLK68_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90464.1"
                     /translation="IMYPVRVALTFKSFTPGGAVEIAHILGKNESLKRIDLAIKLLSM
                     K"
     gene            474..549
                     /locus_tag="PLK68_08415"
     tRNA            474..549
                     /locus_tag="PLK68_08415"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:507..509,aa:Glu,seq:ctc)
     gene            complement(607..1491)
                     /locus_tag="PLK68_08420"
     CDS             complement(607..1491)
                     /locus_tag="PLK68_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90465.1"
                     /translation="MLIIHKLEHTHFSSSEAIIIDYILEQGLKIKNMSANSIAKATFT
                     SAPLLVRIAKKLGYSGWNAFKEDFIAELEYLFKEQKVDASIPFVVSDDIMTISNNIGQ
                     LQIDAIQDTMSMLNHDDLQTALRYLRNAKELDLYGVSNNLLLAEGFRSKLFYIHRNVN
                     ICRLPGNPKVQAAMSDETHCAILISYSGETEFIIEVAKVLKKKETPIIAITCIANNRL
                     SKIADVTLRISSREMLHTKIGDFATTTSIKYLLDTLYAGIFSFDYQKNLEYKIQIAKA
                     VDDRHSGYEFIDEDEFPQ"
     gene            1701..1946
                     /locus_tag="PLK68_08425"
     CDS             1701..1946
                     /locus_tag="PLK68_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HRM90466.1"
                     /translation="MESRIAILGIFVYELDSANKINDLLHKYSRYIIGRMGIPYKDKQ
                     VSVMSVIVDGPNDVIGALSGKIGMLKGVSVKALYSKG"
     gene            1948..3006
                     /gene="hydE"
                     /locus_tag="PLK68_08430"
     CDS             1948..3006
                     /gene="hydE"
                     /locus_tag="PLK68_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRM90467.1"
                     /translation="MSNIDLINKLHQEGALDFDEFKRLLKTFDQNDFDYAKKIAAKIT
                     EQIFKNKIYIRGLIEISSYCKNDCYYCGLRHSNKLALRYRLNLEEILLCCKEGYRLGF
                     RTFVLQGGEDEYYQDERMVEIIEKIKELYPDCAITLSLGEKSKETYQKYFNAGADRYL
                     LRHETYNREHYYQLHPHEMSFDNRIDCLLNLKAIGFQTGCGFMVGSYHQDIDCLVNDL
                     LFIKELKPEMVGIGPYLVHQNTPFKNQPNGDLKLTLFLLSLIRIMTKDVLLPATTALA
                     TLDSNGRLEGINHGCNVVMPNLSPQEVRKKYSLYDGKAASGQEASEGLKELIDTLKTA
                     GYEVVYERGDYCKFDYEG"
BASE COUNT         1103 a          410 c          584 g         1071 t
ORIGIN      
        1 ggattatgta tcctgtcaga gttgctttga cttttaagtc atttactccg ggcggagctg
       61 ttgaaattgc acatatttta gggaaaaatg aatcacttaa aagaatcgat ctggccatta
      121 agttattaag tatgaaataa tttgatactt aaattgaaat agtattttga atatacataa
      181 aagccttttt tacaggaatt ttgtttctat taaaaaggct tttaaaagtg gcatatatta
      241 ctgttttatt agctctgatt gctttaaagt tggtacttat tattataatg aatataagtt
      301 ttataattat tgatgttgat atttctgggt ccatgtctaa aatatactca actgtataga
      361 taattttgcc atagattcac tacactatct aaaacatcta ataattatat tgataaaaat
      421 tgttttgaat aatgattgtc ttttttttat aaatagtgct ataatattta catggtctgt
      481 tggtgaagcg gtcaacacac taggttctca tcctagcatt cacgggttcg aaccccgtac
      541 aggctaccaa ataaaaataa cacccacgta aggggtgttt ttttgtttgg ttttgaattg
      601 acgagtttat tgaggaaatt catcttcatc aataaactca taacccgagt gacggtcatc
      661 aacagcttta gcaatttgaa ttttatactc aagatttttt tgataatcaa atgaaaaaat
      721 tcctgcataa agagtatcca gtaagtattt aatcgatgtt gttgttgcaa aatctccaat
      781 ctttgtatgt aacatttcac gtgatgaaat tcgtaatgtt acatcagcaa ttttagaaag
      841 gcgattattt gcaatacagg taatcgcgat gattggagtt tctttctttt tcaatacctt
      901 agcaacctca ataataaatt ctgtttctcc agaatatgaa attaaaatag cacaatgggt
      961 ttcatcactc attgccgctt gaaccttggg attgccaggc agacgacaga tatttacatt
     1021 acgatgaata taaaataatt tacttctaaa tccttctgct aataaaaggt tattagaaac
     1081 tccatagaga tctaattctt tagcatttcg aagataacgt aaggccgttt gtaagtcatc
     1141 atgattcagc atggacatcg tgtcttgaat cgcatcaatt tgaagttgac cgatattatt
     1201 agaaattgtc ataatatcat cactaacaac aaaaggaata ctagcatcga ccttttgttc
     1261 tttaaataaa tattcgagtt ctgcaataaa atcttcttta aaggcgttcc agccggaata
     1321 acctaatttt ttggcaatac gtactaaaag tggggcagaa gtgaatgttg ctttagcaat
     1381 actgtttgct gacatattct ttatttttaa accttgttca agaatgtagt caataataat
     1441 agcttcactt gatgaaaagt gagtatgttc aagtttgtga attattaaca tattttttac
     1501 ctcgctttat ttcattatac gtgtcgaaga caaaagagta aaccaaaaag aattaaaatg
     1561 ataacgtttt catttcaaat gtggtagaat ataagtatgc agctaatgat gaggaaattt
     1621 ctgagtcaaa gggtgaacaa ttatttttgt tttgttcaac cattagtacg ccctaatggt
     1681 ttttagttta aggaggtaaa atggaaagta gaattgcaat tttaggaatt tttgtttatg
     1741 aattagacag tgctaataag attaatgact tgttgcataa atatagtcgc tatattattg
     1801 ggcgaatggg aattccctat aaagataagc aagtatcagt tatgtctgtt atcgttgatg
     1861 gaccaaatga tgtaataggg gctttatcgg gaaaaatcgg aatgctcaaa ggtgtcagtg
     1921 taaaggcact atattcaaaa ggatagaatg agtaatattg atttaattaa caagcttcat
     1981 caagaaggcg cattggattt tgatgagttt aaaagattat taaaaacatt tgatcaaaac
     2041 gattttgatt acgctaagaa gattgctgcg aaaatcactg aacaaatatt taaaaataag
     2101 atctatattc ggggattaat tgaaatcagc agttattgta aaaatgattg ttattattgt
     2161 ggattacgtc acagtaataa attagcatta cgctatcggc taaatttaga agaaatatta
     2221 ttatgctgca aagaaggtta tcgcttaggc tttcgaacat ttgttttaca aggtggagaa
     2281 gacgaatatt accaagatga acgaatggta gaaattattg agaaaataaa agaattatat
     2341 cctgattgtg caattacgct atcgttagga gaaaaaagta aagaaactta tcaaaagtat
     2401 tttaatgcag gtgctgatcg ttatttgctt cgtcatgaaa cttataatag agagcattac
     2461 tatcagcttc atcctcatga gatgtcattt gataatcgaa ttgactgtct tttgaattta
     2521 aaagcaattg ggtttcaaac aggatgtgga tttatggtag gaagttatca tcaggatatt
     2581 gattgtctgg ttaatgattt attgtttatt aaagagctaa agccggaaat ggtagggatt
     2641 ggaccatatt tagtgcatca aaatacgcct tttaaaaacc aaccaaatgg tgatttaaaa
     2701 cttactcttt ttttattaag tttgattaga attatgacaa aggatgttct attgcctgca
     2761 acaactgctt tagcaacttt agattctaat gggcgcttag agggaattaa ccatggttgt
     2821 aatgttgtga tgcctaattt atcacctcag gaggtaagaa aaaaatactc gttatatgat
     2881 ggaaaagcag cgagtggaca agaagcgagt gaaggattaa aagaattaat tgatacgcta
     2941 aagacggcgg gatatgaagt tgtatatgag cgtggagatt attgtaaatt tgattacgag
     3001 ggttagacct aagtaaaaga aagtgagaaa aaggaaaatg tataatgtaa aatctaataa
     3061 ggctgaagaa tttattgatg atcaagaaat actggaaaca attgcatatg ctaaagaaaa
     3121 taaagataat cgagaattaa tcttttcttt aattgagcgt gctaaaga
//
LOCUS       DAOMTA010000290         3168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1221_length_3168_cov_2.264696, whole genome shotgun sequence.
ACCESSION   DAOMTA010000290 DAOMTA010000000
VERSION     DAOMTA010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_3168_cov_2.264696"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLK68_08435"
     CDS             complement(<1..185)
                     /locus_tag="PLK68_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM90468.1"
                     /translation="MSGFFGVASKESCVLDLFFGIDYHSHLGTRRGGMAVHGNNGFQR
                     SIHNIQNSPFRTKFEKD"
     gene            complement(317..697)
                     /locus_tag="PLK68_08440"
     CDS             complement(317..697)
                     /locus_tag="PLK68_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90469.1"
                     /translation="MPEHKLISLCLETSDRIVVNVTLLKEASHYKMRTEYLKEDDKIK
                     ETNDEVARNLVYQLFGNLEIADFEGLNDLEANSIGSLKVYYGSHDYLERHFDQETLKK
                     SNELKEVIISLLEFVDNSIDKQMF"
     gene            complement(873..2258)
                     /locus_tag="PLK68_08445"
     CDS             complement(873..2258)
                     /locus_tag="PLK68_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90470.1"
                     /translation="MMSDILITANNISKIYDQDILLKRGANFYALRNVDFILEEGDFI
                     SVMGPSGSGKSTLLNCLSTLDEVSSGAVKIFNRFVGEMNDAELSDYRNRYLGFIFQNH
                     NLVPSLSVFDNIATPLILNEVSPQEIKERVNEIGERLNISHTLYKKPNECSGGERQRV
                     AIARAIVTKPKIVVCDEPTGNLDSKNSHEVLEILRELNQQGTSIILVTHDNMIASYAK
                     KFLYLRDGQIINRIDCQNGSQVDFFNEIVKITTQDSLLKLFNPQNNLKKESKKEFQEK
                     EIKNSVGEKTEDRKNENTTSQVKVETFEPKKSTTARLIVYAIFDGLEYDEASALRNRV
                     LNFDKDAVRFNNTNYEEIEIPYGEIKSTKISMCSRSIISWPMPEFRFYLDLDIITKDN
                     NICRFEVLNQRNALSLFDTLEKHQIVMEDPYGIVTLYRSKPDDLVRHRYLLYNFKKIA
                     KEYNLDNPRNL"
     gene            complement(2227..>3168)
                     /locus_tag="PLK68_08450"
     CDS             complement(2227..>3168)
                     /locus_tag="PLK68_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90471.1"
                     /translation="VITVDLTNILIIYGALFLTKMIYVVVIDMGKFMRIKTDIAGYMN
                     HVPRKTTNFNPFKLMSSGLALKGEKPKTIDMLPKGKILSTILGILLIYIGFTPMLRSI
                     ENNNLPTYFAFSLLGEVLIIDNTFPLLFDLLHNKVLLKSKNWIISLSNLKDLTDVMTA
                     MINISAVVVPIIISFLFLQSVSIDVQNAMMMSFFALMASMFLCFIIRFMIYLPTRASV
                     IATMRALGYNKKSIFKIHYQEMMLFIILIVIFPIVMYGSLLYQSYLVNMITYNTFITM
                     IAIYIILYTFMSIYMIVSYRKLIKEVYDDVRYLNHGE"
BASE COUNT         1103 a          557 c          372 g         1136 t
ORIGIN      
        1 atatcttttt caaatttagt tcgaaacggc gagttttgaa tgttatgaat cgatctttga
       61 aaaccattat ttccatgaac tgccattcct cctcgtcttg ttcctaaatg ggaatgataa
      121 tcaatcccaa aaaacaaatc taaaacgcaa ctttcttttg atgcaacacc aaaaaatcca
      181 ctcatttata tctatcctcc aaactttata cacccattgt atcttatttg taacaaattt
      241 cctatattta atcttatttt cgctaaaata ttgcccagga aataaagaaa aacctgttat
      301 aataacaggt aaaatatcaa aacatttgct tatcaatact attatctaca aattctaata
      361 aagaaataat tacttctttt aactcatttg acttctttaa agtttcttgg tcaaaatgtc
      421 tttccaaata atcatgacta ccataataga cttttaaact accaatagaa ttagcttcta
      481 gatcatttaa cccttcaaaa tcagctattt ctagattacc aaaaagctga taaacaagat
      541 ttcttgctac ttcatcatta gtttctttga ttttatcatc ttcttttaaa tactctgtcc
      601 tcattttata atgagatgct tctttcaaca acgttacatt cacgactatt ctatctgagg
      661 tttctaagca caaagaaatt agcttatgtt ctggcaacta aattcctcct tcactaatac
      721 taaattatat gaaaattata tcttattata ccccattagt tagataataa tcataaaaaa
      781 actaggtata agagtatacc tagtatcaaa tatatttcta tcctatcaat tcaatatttt
      841 tttaatctta ttttattagc tgctgactgc ctttataaat ttcttgggtt atcaagatta
      901 tattctttag caattttttt gaaattatat aaaaggtagc gatgacgtac taaatcatcc
      961 ggcttactac gatacaatgt tacaattcca taaggatcct ccattacaat ttgatgcttc
     1021 tccaatgtat caaataaact taatgcattt ctttgattta acacttcaaa gcgacatata
     1081 ttattatctt tagtaattat atctagatca agataaaatc taaattctgg catcggccat
     1141 gaaattattg atcttgaaca cattgagatt tttgtactct taatttcacc gtaaggaatt
     1201 tcaatctcct cataattagt attattgaac ctgacagcat ccttatcgaa atttaaaaca
     1261 cgattacgta atgcagacgc ttcatcatat tccaatccat caaaaattgc ataaacaatc
     1321 aatcgtgcag tagtagattt tttgggctca aacgtctcaa cttttacttg actggtagta
     1381 ttttcattct tcctatcttc tgttttttca ccaacagaat tttttatttc cttctcttga
     1441 aattcttttt tagattcctt tttaagatta ttttgaggat taaacaattt taataaacta
     1501 tcctgtgttg ttatcttaac aatctcatta aaaaaatcaa cttgactgcc attttgacaa
     1561 tcaatccgat taataatttg tccatcacgt aaataaagaa atttctttgc ataactggca
     1621 atcatattat catgagttac taagataatt gaggttcctt gttgattcag ttctcttaag
     1681 atttctagta cctcatgtga atttttagaa tccagattcc ctgttggttc atcacaaaca
     1741 acaatttttg gcttcgttac tattgctcta gcaatagcaa cacgctgccg ttctccacca
     1801 gaacattcat ttggcttttt atatagagta tggctaatat ttaagcgttc accaatctca
     1861 ttaacgcgtt ccttaatttc ttgtggagaa acttcattta aaatcaaagg tgtagcaata
     1921 ttgtcaaaaa cagataatga tggaactaaa ttgtgatttt gaaaaatgaa tcccaaatat
     1981 cgattacgat agtcacttag ttcagcatca ttcatctcac cgacaaaacg attaaatatt
     2041 ttaactgctc cactacttac ttcatctaat gttgataaac aatttaataa tgttgatttt
     2101 ccggaaccac ttggccccat aacactaata aaatcccctt cctctaagat gaaatcgaca
     2161 tttcgcaatg cataaaaatt agcgccacgt tttaataaaa tatcctgatc atatatttta
     2221 ctaatattat tcgccgtgat taagatatct gacatcatcg tacacctcct tgattaattt
     2281 acgataactg acgatcatat atattgacat aaatgtatat aaaataatat aaattgcaat
     2341 cattgtgata aaagtattgt aagtaatcat attaactaaa taactttgat ataataagga
     2401 tccatacata acaattggga atatcacaat taaaataata aacagcatca tctcttggta
     2461 atgaatctta aaaatacttt ttttgttgta acctaaagcc cgcattgttg caattactga
     2521 agctcgggtt ggcaaataga tcataaagcg aataataaaa caaaggaaca ttgacgccat
     2581 taatgcaaaa aaactcatca tcattgcatt ttgcacgtca atcgaaactg actgcaagaa
     2641 taagaaacta ataattattg gtacaacaac agctgaaata ttgatcattg ctgtcattac
     2701 gtcagtaaga tcttttagat tactaagtga tatgatccag ttctttgatt ttagtaagac
     2761 tttattatgc aataagtcaa ataataaagg aaatgtatta tcaatgatga ggacctcacc
     2821 taataatgaa aaagcaaaat aagtaggtaa attattattt tcaattgatc ttaacattgg
     2881 cgtaaaacct atatatatta gtaatattcc tagtattgtt gataatattt ttccttttgg
     2941 aagcatatca atagtttttg gcttttcccc ctttaaagct aaaccacttg acattaattt
     3001 aaatggatta aaattagttg tttttcgtgg cacatgattc atatagccag caatatcagt
     3061 tttgatcctc ataaattttc ccatatcaat aacgaccaca tagatcattt ttgttaagaa
     3121 taaagcgcca taaataatta aaatattcgt taggtcaact gtaatcac
//
LOCUS       DAOMTA010000291         3167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1222_length_3167_cov_3.832262, whole genome shotgun sequence.
ACCESSION   DAOMTA010000291 DAOMTA010000000
VERSION     DAOMTA010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1222_length_3167_cov_3.832262"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLK68_08455"
     CDS             <1..213
                     /locus_tag="PLK68_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90472.1"
                     /translation="SYLRKRANYCLTMCSKENIVSKIETYSSKLSSDYLMDLIFSILF
                     QKDYYPNLIRKINCEQKYEKKTDHDM"
     gene            339..1697
                     /locus_tag="PLK68_08460"
     CDS             339..1697
                     /locus_tag="PLK68_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90473.1"
                     /translation="MDKMADVLGRVGAWCGQNKYLSAIKNAFQTFMPLTIAGAIGVLW
                     CNVLVNADSGLGMVWKPIMALEVINPAFAAMQFATISCITVGITFGIAQEIGEANGET
                     GYFAGLLGLACWLAVTQSGWANYALVDTAKQTLELTADGALQTFTGVAGGALGATGLF
                     TGMIVGVVSVEIFCALRKVEGLKLKMPETVPPGVARAFEVLIPAVITLAIIALIGRGC
                     ELATGLYLNDVISTYIQGPLGAIGSTIPGVIIIYIIVMLFWLVGIHGNNMLAAVKEAL
                     FTPLMLENIETFSKTNDAKSDELHIFATAWLQMFGEFGGSGVTIGLVIAIMIFSKRED
                     NRTIAGISLVPGLFNINETVTFGIPMVLNPILGIPFVLAPIVTLMLGYVLTVIGFCPK
                     AVINTPWTTPPILHGFLTTGANIMGAVSQAIAIVASILIYAPFLIAYERYQNKQAAEA
                     AE"
     gene            2308..2481
                     /locus_tag="PLK68_08465"
     CDS             2308..2481
                     /locus_tag="PLK68_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90474.1"
                     /translation="MREKVISLEPLSLKMATHKGVKILCSSVIRISERVWLMHRIYDE
                     QNIIILDEELTKN"
     gene            2787..3086
                     /locus_tag="PLK68_08470"
     CDS             2787..3086
                     /locus_tag="PLK68_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90475.1"
                     /translation="MRLLSIIKVIVVMIDARSEWNVKDGQTKFYEYYLKRVKPEYQDA
                     AQHLLKESFKRQADGNFDDNFINKFVKETQVYIETDKLTEVLEIIEHFCTGHFNN"
BASE COUNT         1094 a          414 c          583 g         1076 t
ORIGIN      
        1 agttatttaa gaaaacgggc gaattattgc ttaacaatgt gtagtaaaga aaatattgtt
       61 tcgaaaatag aaacctattc ttcaaagtta agtagtgatt atttaatgga tctaatcttt
      121 tcaatcttat ttcaaaaaga ctattatcct aacttaatta gaaagataaa ttgtgaacaa
      181 aaatatgaaa aaaagacaga tcatgatatg taataaattt acatatcatt tttaaatatt
      241 ttaaaaaaat tgtaagactc ttacagaatg tggggaaagc gtttccaagt atttcaccct
      301 ttgctattat taggtagtca ataagggagg aaaaaaatat ggacaaaatg gcagatgtcc
      361 ttggccgcgt tggtgcgtgg tgtgggcaaa ataagtattt atctgcaatt aagaatgcgt
      421 tccaaacatt catgcctttg actatcgcag gtgcgatcgg ggttttatgg tgtaacgtat
      481 tagttaatgc agattcaggg ttaggtatgg tctggaaacc tatcatggct ttagaagtaa
      541 ttaacccagc gtttgcagca atgcaattcg caacaatttc ttgtatcaca gttgggatta
      601 cttttggtat cgctcaagaa atcggtgaag ctaatggtga aactggatac ttcgcaggtt
      661 tattaggact tgcttgctgg ctagcagtaa ctcaaagtgg atgggctaac tatgcattag
      721 tagacactgc taaacaaaca ttagaattaa cagctgatgg agcattacaa acatttacag
      781 gtgttgctgg tggtgcttta ggagcaacag gtttatttac agggatgatc gttggggttg
      841 tttcagtaga aatcttctgt gcattacgta aagtagaagg attaaaatta aaaatgcctg
      901 aaacagtacc tccaggtgtt gctcgtgcat tcgaagtttt aattccagct gtaattactt
      961 tagcaatcat tgcgttaatt ggacgtggat gtgaattagc tactggatta tatttaaatg
     1021 acgtaatttc aacttacatc caaggacctt taggtgcaat tggatcaact attccagggg
     1081 taatcattat ttatatcatt gtcatgttat tctggttagt aggtattcat ggtaacaaca
     1141 tgttagctgc agtaaaagaa gcattattta caccattaat gttagaaaat atcgaaacat
     1201 tctctaaaac aaatgatgca aaaagcgatg aattacacat tttcgcaacg gcatggttac
     1261 aaatgttcgg tgaatttggt ggttctgggg ttacaatcgg tttagtaatc gcaatcatga
     1321 ttttctcaaa acgtgaagat aaccgtacaa tcgcaggtat ctcattagta ccaggtttat
     1381 tcaacatcaa tgaaacagtt acatttggta tcccaatggt attaaaccca atcttaggta
     1441 ttccatttgt attagcacca atcgttactt taatgcttgg ttatgtatta actgtaattg
     1501 gattctgtcc aaaagctgta atcaatacac cttggacaac accacctatc ttacatggtt
     1561 tcttaactac aggagctaat atcatgggtg ctgtatctca agcaattgca atcgtagcat
     1621 ctatcttaat ttatgcacca ttcttaattg cttatgaaag atatcaaaac aaacaagctg
     1681 cagaagctgc tgaataattt gataataaaa agtgtatctt taaaaagatg cactttttta
     1741 aatttcaaaa ataaataaaa taatttataa aaaggttgat taattcatat taaattaaca
     1801 ttcatgagag attataatag tgcaaggtag ataacttgta taaatagtca ctactcccta
     1861 aaatatctat tgacactatt tgttttaatc agtgactatt tttatttttc tattaagccg
     1921 tataggcttt tttattttag taaaataatc ttttcttggc ttttaataaa ctgagttgtt
     1981 ataattcgaa taatcaagta acagtaatgt gtaaatctag atattgggtt tgcgcatttt
     2041 ttatttgcat tttcaagtga ggacgcttaa gcaaacgatt ggggtatttg aaaaaatagt
     2101 agagagtata aaaactggtt atacggccac taattagagt actgctcagt tgtactggtg
     2161 gattaacaac tagttcttta cgatatctta tagtaagcta tttgcagtag gaaatcacta
     2221 tgatgtaatt gttttaagca ccacaaattt ttactaaata tgatgctaga catgctctta
     2281 cattgattga agataagatt gatcatattg agggaaaaag ttatatcact tgaacccttg
     2341 tcattgaaaa tggcaaccca taaaggtgtt aagatcttat gtagctcagt cattagaatc
     2401 agtgagcggg tttggttgat gcatcgtatt tatgatgaac aaaatattat tattcttgat
     2461 gaagaactta ctaagaattg aaccatttta gaagatatat atgatatttt aaatgttata
     2521 ttaataaaat accaagatat tagaaaaatt gggataagta tgccagatat aattaatcat
     2581 ggcagtcttc tcttatttct ggaagatttt gattgttagt gtcttagagc cggggattat
     2641 tgctttttcg tgttaactta cattataggt agataagtta gtttatgaaa tggaaaaata
     2701 tatcttggaa attgtgaaaa tcgttagttt aaaggagtgt attttattag gaaaaatgat
     2761 tttatatgta taagcaatga ataaaaatga gattactaag tattattaaa gtaattgtgg
     2821 taatgataga tgcaaggagt gaatggaatg tgaaagatgg acaaactaag ttttatgaat
     2881 attatttaaa aagagtgaaa cctgagtatc aagatgcagc acagcattta ttaaaagaaa
     2941 gtttcaaaag acaagctgat ggaaattttg atgataattt tattaataaa tttgttaaag
     3001 agactcaagt ttatattgaa acagataaat taacagaagt tttagaaata attgaacatt
     3061 tttgtactgg acattttaat aactgaatca aagtaaagcc tccggttatt aggaggcttt
     3121 acttctattc aaaatctgat tgactactaa tatttttcca agtttcg
//
LOCUS       DAOMTA010000292         3156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1230_length_3156_cov_1.903902, whole genome shotgun sequence.
ACCESSION   DAOMTA010000292 DAOMTA010000000
VERSION     DAOMTA010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_3156_cov_1.903902"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLK68_08475"
     CDS             <1..320
                     /locus_tag="PLK68_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HRM90476.1"
                     /translation="IPYSVCNVTIGLVCSLQVNTFRTTNGLPYASTMCTGNLRSAGQK
                     LSAYLFAHDKDALHHCFRYLIIIFFFIIGAIIATGLINIFGQCALLFCCILLLITLLI
                     LVY"
     gene            complement(340..858)
                     /locus_tag="PLK68_08480"
     CDS             complement(340..858)
                     /locus_tag="PLK68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90477.1"
                     /translation="MSVHENLIEISKSLENLEETGIYDVEKYSCSLKHLTNQLEYFYC
                     HLVKENGRDIDSTYYHLSNRPQVHQLAYFNIGRGFPKELMDGHWCYILKDLGYKMLII
                     PCTSIKGTSANPEFEMDIEVMMSGVKTKSRLQLSEIRCVDMQRLDLRKTFCDVLSSHD
                     DIIKYVKEHLLK"
     gene            1296..>2310
                     /locus_tag="PLK68_08485"
     CDS             1296..>2310
                     /locus_tag="PLK68_08485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HRM90478.1"
                     /translation="MCGITAFSGKEEALPFLLQGLSKLEYRGYDSAGVTLVDKDKLFT
                     IKTKGRLQNLIDRLDQDTPIGCVGIGHTRWATHGVPSNLNSHPHTNNKNTISLVHNGI
                     IENYRELKEQLVAKGYKFHSETDSEVVVHLLDSYYDGDMLKALKKVITHIDGSYALCI
                     VSTLEPDVVYVTKKDSPLVLGTSDCASFGASDIPALLDYTKDVYFIDDFEIAKLCKNK
                     ITFYDAEGNEIQKEITHIPYDNEAAQKGGYDTFMLKEIHEQPYAISETLRGRVEGNDR
                     IILPELEILKERFTTFNKVYFVACGTAYHACLSGANIMERLTGIPTFTQAASEFRYGD
                     PIID"
     assembly_gap    2311..2320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2321..3121
                     /locus_tag="PLK68_08490"
     CDS             <2321..3121
                     /locus_tag="PLK68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM90479.1"
                     /translation="KTLCIFVSQSGETADTMAALRLAKNKGCTTIAVANVLGSTISRE
                     AEATIYTCAGPEIAVASTKAYTTQVIVLLLLAMYVAQTLGKENDIYKDIINGIAKLPK
                     QIENILKDEPLFEKYANYLKNQKDAYYIGRSLDYASVLEGALKLKEVSYIHADAYIAG
                     ELKHGPIALIEEGSVVIAVATQPHIASKTISNIQETIARGAKVILFTLTGEEVGNVDE
                     TYYMPDVNPILQAVLVAIPLQLISYYAAKLKGCDVDKPRNLAKSVTVE"
BASE COUNT         1059 a          519 c          508 g         1060 t
ORIGIN      
        1 agattcccta tagtgtttgc aacgtaacaa ttggtctagt ttgttcttta caagtaaata
       61 catttagaac aactaatggt ttaccgtatg cctcgacaat gtgtactggg aatttgagat
      121 ctgccggtca aaaactatca gcatatttat tcgctcatga taaagatgcg ttacatcatt
      181 gttttcgcta tttgatcatt atcttctttt ttatcattgg agccattatt gctacaggtt
      241 taattaatat ttttggacaa tgtgcgctgt tattttgttg tatattactt ttgataactc
      301 tactcatttt agtttattag cctacttgtt aggctatttt tattttaaca aatgctcttt
      361 tacatacttt ataatatcat catgactgct caaaacatca caaaatgtct ttcttaaatc
      421 aagccgctgc atatcaacgc aacgtatctc actcaactgt aaacgacttt tagtctttac
      481 gcccgacatc atcacttcaa tatccatttc aaattctgga ttagcacttg ttccttttat
      541 agaagtacat ggaataatca gcattttata gccaagatcc tttaaaatat aacaccagtg
      601 cccatccatt aattctttag ggaaaccacg tccgatattg aaatatgcta attgatgaac
      661 ttgtggtcga ttagaaagat gataatatgt actgtcaata tcacgtccat tttctttaac
      721 tagatgacaa taaaaatatt ctagttgatt tgtcaaatgt ttcaatgaac agctgtattt
      781 ttcaacgtca taaatacctg tttcttctaa attctctaat gatttactta tttcaattaa
      841 attttcatgt acactcataa tttatccctc ctattatcat ttactcctaa acttggaaaa
      901 tcacttttat tttttacaat tttttttaat ttattcataa aataattaaa aattatcagg
      961 aaatgagtca atgtaaataa taaaactaat tatttatgct ttatttcttt gcatctatta
     1021 tcaaatactt catattataa tatgggttgc ttttttaatt ttatttgtat ataatagcaa
     1081 ctgttattaa ggagacagaa atacaaaata gcgccaggac taagtaatta gttgacgagg
     1141 gctagactta tcgaaatatt cggcggatgg tctagaggta tgatactacc taaacaatga
     1201 acaaaaataa tgagtaatca ttaaacaaat tcattcggta tcctccattg ataacataat
     1261 aacaattgaa taatcaaaac tatggaggaa tttttatgtg tggaattact gcgttttccg
     1321 gtaaagaaga agctttacca ttcttacttc aaggactttc taaattagag tatcgtggct
     1381 atgattcagc tggtgtaacc cttgtagata aagataaact tttcacgatt aaaacgaaag
     1441 gaagattgca aaatctcata gatcgactag atcaagatac tccaattggc tgtgtcggaa
     1501 ttggtcacac tcgctgggca actcatggag tcccatctaa ccttaattct catccccata
     1561 ccaataataa aaacactatt tcactagtgc ataatggaat cattgaaaat tatcgagaat
     1621 taaaggaaca actagtagct aaggggtata aattccatag cgaaacagat agtgaagttg
     1681 tcgtccatct actagattct tactatgatg gtgatatgct gaaagcatta aaaaaggtca
     1741 ttactcacat tgatggaagt tacgctttat gcattgtttc tactttagag ccggatgtag
     1801 tttatgtaac taaaaaagac agtccattag tattagggac aagtgactgt gcaagttttg
     1861 gggcaagtga tattcctgct ttattagact acaccaaaga tgtctatttt attgatgatt
     1921 ttgaaattgc taaattatgt aaaaataaaa taactttcta tgacgctgaa ggcaacgaaa
     1981 ttcaaaaaga aattacacat atcccttatg ataatgaagc agctcaaaaa ggcggttacg
     2041 atactttcat gttaaaagaa attcacgaac agccatatgc tattagtgaa actttaagag
     2101 gccgagttga aggtaatgat cgaattattt tgcctgaact agaaatattg aaagaacgtt
     2161 ttacaacttt taataaagta tattttgtcg cttgcggaac tgcatatcat gcttgccttt
     2221 caggagccaa tattatggaa cgcttaactg gtattcctac ttttacccaa gccgcttcag
     2281 aatttagata tggtgaccct atcattgatg nnnnnnnnnn aaaactctgt gtatttttgt
     2341 ctcacaatcg ggtgaaactg cagataccat ggctgcttta cgtttagcaa aaaataaagg
     2401 ttgtactacg attgccgtag ctaatgttct tggttcaact atctcgcgtg aagctgaagc
     2461 aacaatttat acttgtgcag gaccagaaat tgctgtagca agtactaaag cctatactac
     2521 gcaagttatt gtcttattat tattagcaat gtatgttgca cagaccctag gaaaagaaaa
     2581 tgatatctat aaagacatta ttaacggaat tgctaaattg cctaaacaaa tcgaaaatat
     2641 tttaaaagat gaaccactat ttgaaaaata tgcgaactat ttaaaaaatc aaaaagatgc
     2701 ctactatatt gggcgaagtt tagattatgc ttccgtccta gagggtgctt taaaactaaa
     2761 agaagtatca tatatccatg ctgatgctta tattgctggt gaattaaagc atggtccaat
     2821 tgctttgatt gaagaaggca gtgtagtaat tgcagttgct acacaaccac atatagcttc
     2881 aaaaacaatt agcaatattc aagaaacaat tgctcgagga gcaaaagtca ttttatttac
     2941 attaaccggt gaagaggttg gaaatgttga tgaaacttat tacatgcccg atgtaaatcc
     3001 aattttacaa gcagtattag ttgcaatccc actgcagtta atcagttatt atgccgctaa
     3061 acttaaaggt tgcgatgttg ataaacctcg taatttagct aaatcagtaa cagttgaata
     3121 aatatataag agcgttgtta tcacgctctt tttgtt
//
LOCUS       DAOMTA010000293         3150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1235_length_3150_cov_2.064943, whole genome shotgun sequence.
ACCESSION   DAOMTA010000293 DAOMTA010000000
VERSION     DAOMTA010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1235_length_3150_cov_2.064943"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /locus_tag="PLK68_08495"
     CDS             <1..3034
                     /locus_tag="PLK68_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90480.1"
                     /translation="TNEDIEAVKKIEQVAQVEYGNRFDALIQVGDNVKGTTVYTNESF
                     DNKVNKIELVDGTVPVADDECLVDNNYASRNKIKVGSKITLTNDNGEKKFKVVGLIND
                     PRYFSTIERGTNTLGDGTNEAFVEILAQGNESLALPQDLYDLRNGTVFNEIRVTLKNS
                     DDNNIFSDDYLSYVKDVNKEIKKVLSGKVAQVNEELAGDANRELEKGEQEYNDGINEY
                     QDGLAQYNNGLSQYESGLKQYQDGLNKYNDGYRQYQNGLKQYQDGKAQYDQGVSDYQL
                     GKAQYDQGISAYNEGINQYNNGLNQYNVGIEQYNQSVVQLQYLIENNLITPEDAAIMQ
                     EKLNETKIQLDATKKTLDASKLELDNNGAVLAATANQLESARITLANTKVTLDSSKAQ
                     LDETAITLTNSKKQLDDSKITLDKTKVLLDETKIKLDEAKTSLDDAKIKLDDARSAID
                     DIPTGKLISLTREESASILSYDSACQSMKAIAVVFPLIFFLVAALVSLTTMTRMVEEQ
                     RVQSGIFRALGYDKKDVINQYLIYAFLATFVASVLGIIAGVYFFPSIIYFLYRKMLFN
                     VGAPIKISFDTFICIQTFLISVAITLLVTYIVTRQELNEMPASLLRPKAPKMGKRIVL
                     ERITFIWKRLSFNQKVTMRNIFRYKKRFFMSIIGIAGCAALIVTGFGIKNSVSTLADK
                     QYGDIFTYDGIVVFDRNLSNDQLKKEKDEFESLSSVKDCSSFYRKTITVVGSKDHYGT
                     LEVFKNNEELSKYTNLENYQTGEKIKLNNQGVVISAKLSELLGVTIGDKINITIDEKD
                     YQVKITGVMLLYFQHHIYMSEEFYRGLTGETPVNNYAYFNLEDDGNRKTVTNYCDKDS
                     NIDSLNYVKGISQAFRDQMGSIDSVVVILIACAGALAFIVLYNLTNINIQERKSEIAT
                     IKVLGFYPREVYDYVFRENLILSAIGSIVGLGIGKVLHMFIINAVEVEVAMFIRSVNL
                     MSYVYAIIITMIFTYLIDFGMRKVLKNIDMVESLKSIE"
BASE COUNT         1141 a          354 c          628 g         1027 t
ORIGIN      
        1 tacaaatgag gatatagaag ctgttaaaaa aattgagcaa gtcgctcagg ttgaatatgg
       61 aaatcgcttt gatgctttga tccaagttgg tgataatgtc aaaggaacga cagtatatac
      121 aaatgagagt tttgataata aggtcaataa aattgaatta gttgatggta cagttccagt
      181 tgctgatgat gaatgtttgg tggataataa ttatgcttca agaaataaaa tcaaagtagg
      241 aagtaagata acattaacta atgataatgg tgaaaagaaa tttaaggttg ttggattgat
      301 caatgatcct cgttatttta gtacgattga acgggggaca aatactctcg gtgatggaac
      361 taatgaggct tttgttgaaa ttttagctca aggcaatgaa tcattagctt taccgcaaga
      421 tctatatgat cttcgtaatg gaactgtttt taatgaaatt cgagtgactt taaaaaacag
      481 tgatgataat aatattttta gtgatgatta tttaagttat gtaaaagatg ttaataaaga
      541 aattaaaaaa gttcttagtg gtaaagttgc tcaggtcaat gaggaattag cgggtgatgc
      601 taatcgtgaa ttagaaaaag gcgaacagga atataatgat ggtatcaatg aataccaaga
      661 tggattagct caatataaca atggtcttag ccaatatgaa agtggcttaa agcagtatca
      721 agatggttta aataaataca atgatgggta tcgtcaatat caaaatggtc taaagcaata
      781 tcaagatgga aaagctcagt acgatcaagg agtcagtgat tatcagctgg gaaaagctca
      841 gtatgatcaa ggaatcagtg catataatga ggggataaat caatataata atggactgaa
      901 tcagtataat gtgggaattg agcaatataa tcaaagtgta gtacaattac aatatttaat
      961 tgagaataat ttaattacgc cagaagatgc tgcaataatg caagaaaagt tgaacgagac
     1021 taaaattcaa ctggatgcaa ctaaaaaaac tttggatgcg agcaagctgg aattggacaa
     1081 taatggtgct gttttggctg ctacagctaa tcaattggaa agtgcaagaa ttacgttagc
     1141 aaatacgaaa gttactttag acagctccaa agcccagtta gatgaaacag cgataacttt
     1201 gactaattct aaaaaacaac tggacgattc taaaattaca ttagacaaga caaaagtttt
     1261 actggatgaa acaaagatta aattagatga agcgaaaaca tcgttagatg atgctaagat
     1321 aaaactggat gatgctcgca gtgccattga tgatattcca actggaaaac tgattagttt
     1381 aacacgtgag gaaagtgcta gtatcttaag ctatgattct gcttgtcaat caatgaaagc
     1441 cattgcagta gtctttccac tgatcttttt cttggtcgct gcattagtta gtttaacaac
     1501 aatgactcga atggttgaag aacagcgagt ccaaagcgga atcttcagag ctttaggata
     1561 tgataaaaaa gatgtaatta atcaatattt gatttatgct tttttagcta ctttcgtggc
     1621 tagtgtatta ggaattatcg caggagtata tttcttccca agtattattt atttcttgta
     1681 tcgtaaaatg ttgtttaatg tcggtgcacc aatcaagatt tcatttgata cttttatttg
     1741 tatccaaaca tttttgattt cagtagcgat tactttatta gttacttaca tagtgacacg
     1801 tcaagaatta aatgagatgc cagctagtct gcttagacct aaggccccta aaatgggaaa
     1861 aagaattgtt ctagaaagaa taacatttat ttggaaacgt ttgtctttta atcaaaaagt
     1921 tacgatgcga aatattttta ggtataaaaa gcgtttcttt atgtcgatta taggaattgc
     1981 tggttgtgcg gctttaattg ttactggatt tgggatcaag aattcggttt ctactttagc
     2041 agataagcaa tatggtgata tttttactta cgatggaatt gttgtatttg atcgtaatct
     2101 aagtaatgat caattaaaaa aagaaaagga tgagtttgag agtttaagta gtgtaaaaga
     2161 ttgcagtagt ttttatcgaa aaacgatcac agttgtagga agtaaagatc attatggtac
     2221 attagaagta tttaagaata atgaggaact tagcaaatat acaaatctag agaattatca
     2281 aacaggtgaa aaaattaaat taaataatca gggggttgtt atttcagcta aattatcaga
     2341 attattagga gtaacgattg gagataaaat aaatataacg attgatgaaa aagattatca
     2401 ggttaaaatt acaggagtaa tgttattgta ttttcaacac catatatata tgagtgaaga
     2461 attttatcga ggtctaacag gtgagactcc agttaataat tatgcgtatt ttaatttaga
     2521 agatgatggc aatcgtaaaa ctgttactaa ctattgcgac aaggatagca atattgattc
     2581 attaaattat gttaaaggaa ttagtcaagc atttagagat caaatgggca gtatcgatag
     2641 tgttgttgta atattgattg cttgtgccgg ggccttagct tttattgtct tgtataatct
     2701 aacaaatatt aatattcaag aacgtaaaag tgaaattgca actataaagg tattaggctt
     2761 ttatccacgt gaggtgtatg attatgtatt tagagaaaac ctaattttat cagcaatcgg
     2821 atcaattgta ggattaggaa ttgggaaagt tttacatatg tttatcatta atgcggtaga
     2881 agttgaagta gctatgttta ttagaagtgt taatttaatg agttatgttt atgcaataat
     2941 tattacaatg atctttactt atttaattga ttttggaatg cgaaaagttt taaaaaatat
     3001 tgatatggtt gagtcattaa agagcattga ataaggtatt tttagttata aattctggtt
     3061 aatattatta ttaatattgc ctttttagtg gtatatgatt ataatttatt tatcagcaac
     3121 ggagaccata aatggctact tttaaagact
//
LOCUS       DAOMTA010000294         3141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1238_length_3141_cov_2.098185, whole genome shotgun sequence.
ACCESSION   DAOMTA010000294 DAOMTA010000000
VERSION     DAOMTA010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1238_length_3141_cov_2.098185"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="holA"
                     /locus_tag="PLK68_08500"
     CDS             <1..663
                     /gene="holA"
                     /locus_tag="PLK68_08500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRM90481.1"
                     /translation="IFVIYHVGKDFDERKKIVKNLRKQAQFFEIDKVNHYKLSDSTRQ
                     AIRRRDATIDDDALELLLSRLGDSLNNVALEVEKLCLYSKHITYDIVDELVSKPLDEN
                     VFALTSAILQKDRRKMFSIYHDLMILNEEPIKLIVLIAGQMRLIYQVKLLDRKGYNDK
                     EIGKILGVNPYRLKYVRQEGKDFDLNELLQCLDDLSRLDVEIKTGKMDKKMGLELFMV
                     KI"
     gene            672..1886
                     /locus_tag="PLK68_08505"
     CDS             672..1886
                     /locus_tag="PLK68_08505"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRM90482.1"
                     /translation="MESLRELYKIGVGPSSSHTMGPQRAALKVKETYPQATHFEVYLH
                     GSLALTGKGHLTDYIIEETLGKDSVKIHFVNTALPKHPNGMIFEIYRENQLLDKITVY
                     SVGGGALMYDDSSLQPSKQVYPHGNLTEILEYCDRKGINLYDYTIEYEDEHFKAYLFE
                     VLNAMFACVEAGLSTTGVIPGKLGLKRVAKSMYQQAINTRRSGERDRLLVSSYAYAVS
                     EENASGHRIVTAPTCGASGVLPAVLYYCYKQLEIPRKEIIKALAVAGIFGNVIKTNAT
                     ISGADGGCQAEIGSACAMAAAAYGWILELNNNLIQYAGEMGLEHNLGLTCDPVGGYVQ
                     IPCIERNGFGALRAMDAAMYAKQLGYLRKNKVSFDAVVRVMKETGKDLNSAYKETSLG
                     GLAKEFGFENED"
     assembly_gap    2028..2037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2038..>2308
                     /locus_tag="PLK68_08510"
     CDS             <2038..>2308
                     /locus_tag="PLK68_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM90483.1"
                     /translation="KTIYIGGGTPSSLNDEQLEWLMDIIKPYISSETKEVSIEVNPES
                     IDYYKLDILKRGGVSRLSIGVETFNDILLKRINRQHTTIQVERIID"
     assembly_gap    2309..2318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2319..2963
                     /locus_tag="PLK68_08515"
     CDS             <2319..2963
                     /locus_tag="PLK68_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HRM90484.1"
                     /translation="NISIDLMYGLPNQTITDIKNDLAKVRQLPIEHISYYSLILEDHT
                     VLKNLNYQPLDEEVESKITQLIEESLEQIGFHKYEISNFAKTGYESLHNLAYWQYDNY
                     YGIGVGASGKIDDCLIEHNRNLNAYLRRQNTITKMINSKEETMFNHLMMSLRLVKGLD
                     LKEFEKRYGLRAVDVYQTAIDKHLKMKTLVIENDYLHATSESIKLLNEILIDFL"
BASE COUNT         1101 a          367 c          639 g         1014 t
ORIGIN      
        1 atatttgtaa tttatcatgt tggtaaagat tttgatgagc gtaaaaaaat tgttaaaaat
       61 ttacgtaaac aagcacaatt ttttgaaata gataaagtta atcattataa gctatcagac
      121 agtacacgtc aagcaatcag acgtcgagat gcaacgatcg atgatgatgc attagaattg
      181 ttattgagtc gtttaggaga tagtttgaat aatgttgctc ttgaggtgga aaaactatgt
      241 ttgtacagca aacatattac atatgatatt gttgatgaat tggtaagtaa gccgttggat
      301 gaaaatgttt ttgcattaac tagtgcgatt cttcaaaaag atcgaagaaa aatgttttcg
      361 atttatcatg atctaatgat tttaaatgag gaaccaatta agttgattgt tcttatagcg
      421 ggtcaaatgc ggctaattta ccaagttaaa ttattggatc gtaaaggtta taatgataaa
      481 gaaattggaa aaatactggg agttaatcct taccgattaa aatatgttcg tcaagaagga
      541 aaagattttg atttaaatga attattgcaa tgtttagatg atttatcacg tcttgatgta
      601 gaaatcaaaa caggtaaaat ggataaaaaa atgggtttag aattatttat ggtaaaaatt
      661 taggaggtat tatggaatca ttaagagagt tatataaaat tggggttgga ccatcttcgt
      721 cccatacgat gggaccacaa agagcggcat taaaggttaa ggaaacctat cctcaggcta
      781 cacattttga agtatatcta catggctcgt tagcattgac agggaaaggc catttaacag
      841 attacattat tgaagaaact ttaggtaaag atagtgttaa gatacacttt gttaatacag
      901 cattacctaa acatccaaat gggatgattt ttgaaatata tcgggaaaat caattgcttg
      961 ataaaattac agtttattca gtaggtggtg gagctttaat gtatgatgat agttctttgc
     1021 aaccttctaa gcaagtatat ccgcatggta atttaacgga aattttagaa tattgtgatc
     1081 gtaaaggaat caatttatat gattatacaa tcgagtatga agatgagcat tttaaagctt
     1141 atttatttga agtactaaat gcaatgtttg cttgtgttga agctggatta tcaacaacgg
     1201 gagttattcc aggaaaactg ggattgaaaa gagttgctaa atcaatgtat caacaagcaa
     1261 tcaacacacg tcgtagtgga gaacgagatc gcttattagt tagtagttat gcttatgcgg
     1321 ttagtgaaga aaatgccagt ggacatcgta ttgttactgc accgacatgt ggagctagcg
     1381 gagttttacc agcagtctta tattattgtt acaaacaatt agaaattcct cgaaaagaga
     1441 ttataaaagc attagcagta gctggaatat ttggcaatgt tattaaaaca aatgcaacta
     1501 tttctggcgc tgatggtggt tgtcaggcag aaatcggaag tgcttgtgca atggcagcgg
     1561 ctgcttatgg ctggatttta gaacttaata ataatctgat tcaatatgct ggtgaaatgg
     1621 gcttagaaca taatcttggt ttaacatgtg atcctgtagg cggctatgtt caaattcctt
     1681 gtattgagcg taatggtttt ggtgcactta gagcaatgga tgcggcgatg tatgctaagc
     1741 agctgggtta cttgagaaaa aataaagttt cttttgatgc ggttgttcgg gtgatgaaag
     1801 aaacaggaaa agatttaaat agtgcgtata aggaaacttc tttaggtggt ttggctaaag
     1861 aatttggttt tgaaaatgaa gactaattca ttatatctgc atattccttt ttgtgaacaa
     1921 atttgtgcat attgtgactt ttgtaaagtt ttttataacg aacatcaagc cgatgattat
     1981 ttggcggttt taaagcatga acttcaagct ttaaatatta cagagccnnn nnnnnnnaaa
     2041 gactatatat attggtggtg gaacaccgag cagtttaaat gatgaacaat tagaatggct
     2101 gatggatatt attaaaccat acataagtag tgagactaaa gaggtgtcca tagaagttaa
     2161 tccagaaagt atcgattatt ataaattaga tattttaaaa agaggtggtg ttagccgttt
     2221 aagtatcggg gttgaaacat ttaatgatat ccttttaaaa aggattaatc gccagcatac
     2281 gactatacaa gttgagcgga ttatcgatnn nnnnnnnnaa tatttcaatt gatcttatgt
     2341 atggtttgcc taatcaaacg attacggata taaaaaatga tttagctaag gttagacagt
     2401 taccgatcga gcatatttct tactactcac taattttaga agatcatact gtcttgaaaa
     2461 atctaaatta tcaaccgctt gatgaagagg tggaatcgaa aatcactcaa ttgatagagg
     2521 aatctttaga acaaataggt ttccataaat atgaaatcag taattttgcg aaaactggat
     2581 atgagtcact tcataaccta gcttattggc agtatgataa ctattatgga attggggtag
     2641 gagccagtgg aaaaattgat gattgcttaa tagaacataa tcgtaattta aatgcttatt
     2701 tacgtcgtca aaatacaatc acaaagatga ttaattctaa agaagaaacg atgtttaacc
     2761 atttaatgat gtcattacgt ttggtgaaag gcttagattt aaaagaattt gaaaagagat
     2821 atggtcttag agcagttgat gtttatcaaa ctgctattga taaacatttg aagatgaaaa
     2881 cgttagtaat tgaaaatgat tatttacatg caactagtga atcaattaag ttattaaatg
     2941 aaatcttaat agatttttta taaacgggga taattagatg agttatcgta tagtgaacgg
     3001 ttaaatcttc taaattgggt ttacaatagg aaataaaaga tgtataatca aatagtaatc
     3061 attactaata aggagattgg gatatgaaca ataaagcatt ttttaagtta agttatgggt
     3121 tgtatgttgt atctagcagt t
//
LOCUS       DAOMTA010000295         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1239_length_3140_cov_1.808752, whole genome shotgun sequence.
ACCESSION   DAOMTA010000295 DAOMTA010000000
VERSION     DAOMTA010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1239_length_3140_cov_1.808752"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>236)
                     /locus_tag="PLK68_08520"
     CDS             complement(<1..>236)
                     /locus_tag="PLK68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90485.1"
                     /translation="LGERSSKEVLKVIDFGISFNNLSLFSKVNFTLYYQEKTVLLGNN
                     GSGKTSFIKALLGNLNNYQGELKMAETVLIGYIP"
     assembly_gap    237..262
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<263..1211)
                     /locus_tag="PLK68_08525"
     CDS             complement(<263..1211)
                     /locus_tag="PLK68_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90486.1"
                     /translation="MIEIGMHKIYKNFGYKQVLKDINFEILTGDCVGIVGKNGSGKST
                     LFKMIMKKEFPDTGNITIRKQATIGYLEQIPTISDNDITVKDMLMSSFFEIHAIEQRM
                     RALEQDMMHNNVDEAILNKYAKIQDQYIALGGYEVEEKLNKVITGFKLHSILDKPFAI
                     LSGDQKTIINLAQLILTEPDILLLDEPTNHLDITMLEWLENYLNKYRGTIVIISHDRY
                     FLDRVTNKTILLDNSENRLFHGNYSYTLKEQERLLLQEFEQYKTQQKKIEAIKASIKR
                     YRDWGNQGDNEKFFKKAKELEKRLEKIEILDKPQLEKKKL"
     gene            1612..2814
                     /locus_tag="PLK68_08530"
     CDS             1612..2814
                     /locus_tag="PLK68_08530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRM90487.1"
                     /translation="MKRKYPKVIISEEGEKWLDKGQMWMYRNNAVNIDETIENGALVD
                     IITTTGRYLGTGFISKNSHITVRILSKDINEIFDRNFFKRRIQFAYDFRKTVEKENLT
                     NCRLVFGEADELPGLTVDRYNDILVCQISSYGLDKIKDMIYEILLEVLTEDGQDVKGI
                     YERNDIKVRAKEGLPLEKGYWRNINLPTTTIINENGINLSVDVENGQKTGYFLDQKAN
                     RVLLRNIAYGKKVLDCFSHTGGFALNAAYGKAKEVVAVDVSQTALDQGYANAKLNNLQ
                     GCISFVKDDVFDYLDKCKKGQFDIIVLDPPAFTKSRRTVDHAYNGYKKINMKAMKLLG
                     RGGYLITCSCSRFMETDNFEKMLRESAHEAGVTLKQVSVTQQNPDHPILWTMEETSYL
                     KFYIFQII"
     gene            2920..>3140
                     /locus_tag="PLK68_08535"
     CDS             2920..>3140
                     /locus_tag="PLK68_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90488.1"
                     /translation="MLCIEFGLFHDFPDHKYKGRYHDYRSIIAKGTIKLIDGNDYYKI
                     KKGYNLLSTCNHREIVPLESRKTILPIYIG"
BASE COUNT         1074 a          421 c          504 g         1115 t
ORIGIN      
        1 tgaggtatat atccaattaa gactgtttcc gccattttta actcaccttg ataattatta
       61 aggtttccaa gaagagcttt aataaatgat gttttacctg aaccattatt tcctagtagg
      121 actgtttttt cctgataata aagtgtaaaa ttcacttttg aaaataaaga tagattatta
      181 aaagatattc caaaatcaat gactttaagc acttctttgc tactgcgttc acccaannnn
      241 nnnnnnnnnn nnnnnnnnnn nngcagtttc tttttttcta attgtggttt atcgaggatt
      301 tctatctttt ctaaacgttt ttctaattct ttggcttttt taaagaattt ttcattatca
      361 ccttgattac cccaatctcg ataacgcttg atagaagctt ttattgcttc gattttcttc
      421 tgttgagttt tgtattgttc aaattcttgt aataataatc tttcttgttc ttttaaggtg
      481 tatgagtaat taccgtggaa taaacgattt tcactgttat ctaaaagaat agttttatta
      541 gttactcgat ccaaaaaata gcgatcatgt gaaataatca caatagtccc acgatactta
      601 tttaaataat tttctagcca ttctaacatt gtaatatcta aatgattagt tggttcatct
      661 aataataaga tatcaggttc agttaaaatt aattgggcta aattaataat tgttttttga
      721 tcaccgctca agatagcaaa cggcttatct aaaatactat gcaatttaaa gccggtaatg
      781 actttgttta gtttttcttc aacttcatag ccgcccaggg ctatgtattg atcttgtatt
      841 ttagcatatt tgtttaatat cgcttcatcg acattattat gcatcatatc ttgttcaagt
      901 gctcgcattc gttgttcaat agcatgaatt tcgaaaaaag atgacatcag catatctttt
      961 actgttatat cattatcact gattgttgga atttgttcta aatagccaat agtagcttgt
     1021 ttgcgaatag taatatttcc tgtatcaggg aattcttttt tcatgatcat tttaaacagt
     1081 gtagatttgc cactaccatt tttgcctaca attcccacac aatcaccagt taaaatctca
     1141 aaattaatat cttttaatac ttgtttataa ccaaaatttt tataaatttt atgcattcct
     1201 atttcaatca ttgtatattt gctcctttat tttataaatt tatttaaaat aaacagagcc
     1261 taagattaat aggatcaggc gattgtttct aatcgcttcg cacagctaga gcattatact
     1321 ctgatcgcct agcctattga ttaacagact ctgtacgaag cacagtgcct tcgactctat
     1381 ggcaaacata acctagaatc atcatgacca cctccctaaa atattattat tttattaatt
     1441 ctatcatatc taaattataa aaacactgaa ttgataatga aaatctttaa gtattggata
     1501 tttagtgatt aatatcgaag aatctattga taaataagaa atatatttgt ttatgaaatc
     1561 gaactatagt agaatagtga agaagaaaat atttataaga gaggtacatt aatgaaaaga
     1621 aaatatccta aggtcattat atctgaagaa ggagaaaaat ggcttgataa aggtcagatg
     1681 tggatgtata gaaataatgc tgttaatatt gatgaaacaa ttgaaaatgg agcgttggta
     1741 gatatcatta ctactactgg ccgttacctt ggaacaggtt ttatttctaa aaatagtcac
     1801 attacggtaa gaattttatc taaagatata aatgaaatat ttgatcgaaa cttttttaag
     1861 cgaagaatac aatttgctta tgactttcgc aagacagtag aaaaagaaaa tttaactaat
     1921 tgtcgtttgg tttttggaga agcagatgaa ctgccgggat taacagttga tcgctataat
     1981 gatattttag tttgccaaat tagttcatac ggattagata aaatcaaaga tatgatctat
     2041 gaaatattac tagaggtttt aacagaagat gggcaagatg ttaaaggtat ctatgaaaga
     2101 aatgatatta aggttagagc aaaagaagga ctgcctttag aaaaaggata ttggagaaat
     2161 attaatttac cgacaacgac gattattaat gaaaatggaa tcaatttaag tgttgatgtt
     2221 gaaaatggtc aaaaaacagg ttacttttta gatcaaaaag caaatcgtgt tttacttaga
     2281 aatatagcgt atggaaaaaa agttttggat tgctttagtc atactggggg ctttgcatta
     2341 aatgcggctt atggtaaagc taaagaagtg gttgcagtag atgtatcaca aactgctctt
     2401 gatcagggat atgctaatgc taaactaaat aatttacaag gatgtatttc ttttgtaaaa
     2461 gatgatgttt ttgactatct agataaatgt aaaaagggac agtttgatat tattgtttta
     2521 gatccacctg cttttacgaa atcgagaaga acggtagatc atgcttataa tggttataag
     2581 aaaatcaata tgaaagcaat gaaattatta ggcagaggtg gatacttaat tacatgtagt
     2641 tgttcacgtt ttatggagac tgataatttt gaaaagatgc ttcgtgaatc agcgcatgag
     2701 gctggagtaa cccttaaaca agtatcagtt actcaacaaa acccagacca tccaatttta
     2761 tggacaatgg aagaaacgtc atatttaaaa ttttatattt tccaaattat ttagattgaa
     2821 taggatttac gaatcgactg aataggcttt taattttaaa gtttaatagt cgatttttat
     2881 ttaggaaaat aatttagatt tttttgaata atatcttatt tgttgtgtat tgaatttggt
     2941 ttgtttcatg attttccaga ccacaagtat aaagggcgtt atcatgatta tcgttcaata
     3001 attgctaaag gaactatcaa gttaattgat ggtaatgatt attacaaaat taaaaaagga
     3061 tataatttat tatccacctg caatcatcgt gaaattgtcc ctttggaaag tagaaagacg
     3121 atactcccaa tatatatcgg
//
LOCUS       DAOMTA010000296         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1241_length_3139_cov_2.306420, whole genome shotgun sequence.
ACCESSION   DAOMTA010000296 DAOMTA010000000
VERSION     DAOMTA010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1241_length_3139_cov_2.306420"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PLK68_08540"
     CDS             complement(<1..950)
                     /locus_tag="PLK68_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90489.1"
                     /translation="MKDKFTALICKVSGNRFLNVLRDSFVLVASLTLIAGFAIMISSV
                     FIDPNGIIFGSSGLGLGEMIFGSEKEFLASGFAQMLVQGQNIFNFFSKGAMSINALLI
                     VVIFSYNVSRKYFSDNREHMVSVMYALAAFFICLPWDFNYTSAKGIEVDINGIINSNF
                     LGQQGIFFGLIVAGSATFIFNKLSKLNISIKMPDSVPPAVAQSFESLVPGMLTLFVFI
                     IATVISNTFAGESLPEFILTTLQAPALAISKTAAFALVSQITWPLFQWLGIHPTAIWG
                     AIFGMTWDIAGNENVLGVAKHLYTTMFMNYSTIAAGTFALA"
     gene            1140..1913
                     /locus_tag="PLK68_08545"
     CDS             1140..1913
                     /locus_tag="PLK68_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90490.1"
                     /translation="MDFKKTVAIHYTEFTPSERKICARILEDPQIVNNSIVVLGDLCE
                     TSKSAILRFYQKLGYRGYSEFKYSVEESFKNNDEKKLSNESLLSQISQEYSNTILELG
                     ALDYDHQLKELASLIDKYPHVLSIGINNTYFAASQLEYSLYSHNRFIQSVREDIQLTY
                     LINSINQDYLCIIYSVSGSTYTYENFIKEASAKGAKIVLITIDGNSELLPLVDLAFVL
                     PSISLPISSIDSIIQIDNRIMLYYFSEIISYYYGLNTQN"
     gene            complement(2034..>3139)
                     /locus_tag="PLK68_08550"
     CDS             complement(2034..>3139)
                     /locus_tag="PLK68_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM90491.1"
                     /translation="FIIKTVAQLTQAVLPGNTSIYKLDSDQMGLLIENSTELEITKLY
                     NEIQKQLLHEQLLKRYKCPIQISAGCAIYPKDGLTYNELNKYADYSLQYAKDNGKNKL
                     TFFSNYILEHKMRSLEILKYIRESVSDDYRGFELNYQPQVEVSSKQIKGVEALLRWQC
                     QELGKVSPVEFIPILEESGLIIPVGLWVIKKAIQACSKWVVYDPEFSVSINVSALQML
                     NSDFVEDVKKVLKKEKLAPKNIVLELTESYMVRNMDLLRTIFEQLRELGFKIAMDDFG
                     TGYASLEILKTVPADIVKIDQTFVRDIKISKFDKTFIRFIAQICHDVDIEVLLEGIET
                     EEEFNVVRFQKMALEEMEQIYEKGKIPLIVGGI"
BASE COUNT         1070 a          600 c          410 g         1059 t
ORIGIN      
        1 gctaaagcaa atgttcccgc tgcaatagtt gaataattca taaacatcgt tgtatataaa
       61 tgtttagcaa cacctaaaac attttcgttt ccagcaatat cccaggtcat tccaaaaatt
      121 gctccccaaa tagcagttgg atgaattccc agccactgga ataatggcca tgtaatttgg
      181 gaaactagag caaacgctgc ggttttagat attgctaaag ctggtgcttg aagcgttgtt
      241 aaaataaact ccggtagtga ttcaccagca aaagtattac taataacggt tgcaataata
      301 aagacaaata atgtcaacat ccccggcact aaagattcaa aactctgagc tacagctgga
      361 ggtaccgaat ccggcatctt tattgaaata tttaacttag ataatttatt aaatataaat
      421 gtagcactac ctgcaacaat cagtccaaaa aagattcctt gttgacctaa aaaattcgaa
      481 ttaataattc cattaatatc gacctcgatt cccttagcac ttgtataatt aaaatcccaa
      541 ggcaaacaaa taaaaaatgc tgctaaagca tacatcactg aaaccatatg ctcacgatta
      601 tcactaaaat attttcttga gacattatat gaaaaaatca ccacaatcaa cagagcgttg
      661 atcgacatag cccctttact aaaaaaatta aaaatatttt gtccttgtac tagcatttga
      721 gcaaatcctg atgctaagaa ctctttctca cttccaaaga tcatctcccc tagaccaagt
      781 ccagatgaac caaaaataat cccattgggg tcaataaata cagaactgat cataattgca
      841 aatccagcaa ttaatgtcag tgaagcaacc aatacaaaag aatccctcaa tacatttaaa
      901 aagcgatttc ctgatacttt acagatcagt gcagtaaatt tatctttcat ttttctctcc
      961 ttttttgttt tagacatctc cggataatgt ccaaaagccc tcttgtgtaa aagcgtttac
     1021 attgcaatta tatcactgtt gagaaattta tcaatattat tttttgttat gggaattttc
     1081 tctattgtaa ttaatttggt ttatcgatat aattataaat aagaaaaaga ggtattaata
     1141 tggactttaa aaaaacagtt gccattcatt atactgagtt tacacctagt gaacgtaaaa
     1201 tttgtgctcg tatcttagaa gatcctcaaa tcgttaataa ctccattgtt gttttaggcg
     1261 acctttgtga aacttctaag tctgcaatcc tacgtttcta tcaaaagctt ggataccgtg
     1321 gatactcaga attcaaatat tctgttgaag aaagttttaa aaataacgat gaaaaaaaac
     1381 tcagcaacga atcattactg agtcagattt ctcaagaata cagtaatacg attttagaac
     1441 ttggtgctct agattatgat catcaactaa aagaactagc atcattgatt gacaaatatc
     1501 cacatgtctt atcaattggg atcaacaata cctattttgc tgctagtcag ttagaatatt
     1561 ctttatactc tcataatcgc tttattcaaa gtgtacgcga ggatattcag cttacctatc
     1621 taattaactc tattaatcaa gattatcttt gtattatcta tagtgtttct ggttctactt
     1681 atacttatga aaactttatt aaagaagctt ctgctaaagg tgcaaaaata gttttaatca
     1741 cgattgatgg taattctgaa ttattacctc tagttgattt agcatttgtt ttaccttcga
     1801 tttccttacc aattagcagt atcgattcta ttattcaaat tgataatcgt attatgttat
     1861 attatttttc tgaaattatc tcctattact atggattaaa tacgcaaaat taaaaatgct
     1921 atgataacat agcattttta atttatatca acttattcgt aatttcttct tcactttgag
     1981 gacgcccaaa caagaatcct tgaatataat ctaattccat cggttcaaca acattaaatt
     2041 cctcctacaa tcaaaggaat ttttcccttt tcataaatct gttccatttc ctcaagagcc
     2101 atcttctgaa aacgaacaac attaaattcc tcttctgttt caattccttc caacaatacc
     2161 tcaatatcaa catcgtggca aatttgagca ataaaacgaa taaaagtttt atcaaattta
     2221 ctaatcttaa tatcacgtac aaacgtttgg tcaatcttga caatatcagc aggaacagtc
     2281 tttaaaattt ccaatgaggc ataccccgtt ccaaaatcat ccattgcaat tttaaatccc
     2341 aattcgcgaa gctgctcaaa aattgttctt agtaaatcca tgttcctaac catatagctc
     2401 tctgttaatt ctaagacaat atttttaggt gccagctttt cttttttcag aacctttttt
     2461 acatcttcca caaaatcact atttaacatc tgaagcgcag atacattaat actaacagaa
     2521 aattcaggat catatacgac ccacttcgaa caagcctgaa ttgctttctt tataacccat
     2581 agaccaactg ggataattag cccgctctct tcaagaatgg gaataaattc aactggtgat
     2641 acttttccta attcttgaca ctgccatctt aacagtgcct caactccttt gatttgttta
     2701 ctactcacct caacttgggg ctgataattt aactcgaagc cacgataatc atcacttaca
     2761 ctttcacgaa tatattttaa aatttctaat gaacgcatct tatgctcaag gatgtagttt
     2821 gaaaagaaag ttaatttatt ttttccatta tccttggcat attgtaatga ataatcagca
     2881 tatttattta gttcattata tgttaaccca tctttaggat aaattgcaca tccagctgaa
     2941 atttgaatcg gacatttata acgttttaat agttgttcat gcaataattg tttctgtatc
     3001 tcattatata atttagtaat ttccaattcg gtactatttt caatcaacag ccccatttga
     3061 tcgctatcca atttataaat actagtatta cctggcaaaa ctgcctgagt caattgggct
     3121 actgttttaa taataaaat
//
LOCUS       DAOMTA010000297         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1242_length_3138_cov_1.842686, whole genome shotgun sequence.
ACCESSION   DAOMTA010000297 DAOMTA010000000
VERSION     DAOMTA010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1242_length_3138_cov_1.842686"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLK68_08555"
     CDS             complement(<1..549)
                     /locus_tag="PLK68_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM90492.1"
                     /translation="MNDLIVFNPLYKNKDIAECFALYTEVVDGKLENSSKFVSLLIKL
                     AEKYNLYDNLFNSLLTYLLINNENSFTLALERKKTIAANLKEIVMNDFKIIFKMFNTD
                     FSVLPLAQQNLITGFTPSKPVINIELFEVSKTLQDNLTACNDVESFYNVLENFFLTYG
                     VGKFGLNKAFRYENEQISPIIHI"
     gene            complement(596..1960)
                     /locus_tag="PLK68_08560"
     CDS             complement(596..1960)
                     /locus_tag="PLK68_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HRM90493.1"
                     /translation="MINLFEVAETNKMIEQENLDVRTITLGISLLDCMDHDIDVLCEN
                     IYKKITTVAKNLVSTGKQIEMKYGIPIVNKRISVTPIGFVGSNACKSTADFVKIAKTL
                     DKCAHETGVNFIGGYTAIVSKGMTKAEELLIRSIPEAMKVTSNVCSSVNVGSTKTGIN
                     MDAVKLMGEIIHETADLTKENDSIGCAKLVVLCNAPDDNPFMAGAFHGVSEADAIINV
                     GVSGPGVVKNAIANAKGKDFGELCEIIKKTAFKITRVGQLVALEASEKLGIPFGIIDL
                     SLAPTPAIGDSIADCLEGMGLDSVGAPGTTAALAILNDQVKKGGVMASSYVGGLSGAF
                     IPVSEDQGMIDAVNRGALTLEKLEAMTCVCSVGLDMIAIPGDTPVTTLSGIIADEMAI
                     GMINQKTTAVRIIPVIGKNVGEVVEFGGLLGYAPVMPVNQFGCAEFINRGGRIPAPIH
                     SFKN"
     gene            complement(1972..2244)
                     /locus_tag="PLK68_08565"
     CDS             complement(1972..2244)
                     /locus_tag="PLK68_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM90494.1"
                     /translation="MQKGIITVVGKDQVGIIAKVCSFLAEKQVNILDISQTIIQGYFN
                     MMMIVELSSITEEFGTICEDLDKLGEEIGVNIKLQHENIFNKMHRI"
     gene            complement(2375..2692)
                     /locus_tag="PLK68_08570"
     CDS             complement(2375..2692)
                     /locus_tag="PLK68_08570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM90495.1"
                     /translation="MATRIMLVCAAGMSTSLLVTKMEVAAKEAGEEVEIFALPLTEGE
                     KIIDTVDCVLLGPQVRYAKKEVEKIIADTGKDIPYDVIEMKDYGMMNGKAVYEFAKKL
                     MGR"
     gene            complement(2788..3102)
                     /locus_tag="PLK68_08575"
     CDS             complement(2788..3102)
                     /locus_tag="PLK68_08575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM90496.1"
                     /translation="MKTILLVCSAGMSTSLLVTKMEGAAKDAGVECKIFALPFSDAPR
                     VLEEVDCILLGPQVRFQKAAIEKLAAGRKAGAIPVDVIDMRDYGTMNGKAVFEAAMKM
                     IG"
BASE COUNT         1019 a          627 c          365 g         1127 t
ORIGIN      
        1 tatatgtatt ataggactaa tttgctcatt ttcataacga aatgctttat ttaatccaaa
       61 ttttccaact ccatacgtta aaaagaagtt ttctaataca ttataaaatg attcaacatc
      121 attacaggca gttaaattgt cctgtaatgt ttttgaaact tcgaatagtt caatattaat
      181 gacaggcttt gaaggtgtga atcctgttat caaattttgt tgtgctaatg gcaagacact
      241 aaaatccgta ttaaacattt tgaaaataat cttaaaatca ttcataacaa tttcttttaa
      301 attagcagct attgtttttt ttctttctaa tgctagagta aacgaatttt cattattaat
      361 taacaaataa gttaataaac tattaaatag attatcgtac aagttgtatt tttcagctaa
      421 tttaattaac aatgaaacaa atttactgct attttcaagt ttcccatcta ctacttcagt
      481 atataatgca aaacactcag caatatcttt atttttatac aatggattaa aaactattaa
      541 atcattcatt ttacattttc ctttcaatat aatgaaaaag acgagttaac gtcttttaat
      601 ttttaaaact atgaattggt gcaggaattc taccaccacg attaataaat tcagcacatc
      661 caaattggtt tactggcatt acaggtgcat aacccaatag tcccccaaat tctactactt
      721 caccaacatt tttaccgatt acaggaataa ttctaacagc tgtggttttt tgattgatca
      781 taccaattgc catttcatcg gcaatgattc cagataaagt agtgactgga gtatctccag
      841 gaatcgcaat catatcaagt ccaactgaac atacacatgt catagcttct aatttttcaa
      901 gtgttaatgc gccacggttt acagcatcaa tcataccttg atcttcactg actggaataa
      961 atgctccact taatccacca acatatgaag aagccataac gccacctttt ttaacttgat
     1021 cattcaagat tgctaatgca gctgttgttc ctggagcacc aacactatcc aagcccattc
     1081 cttctaaaca atcggcgata ctatcaccga ttgctggagt tggagctaat gataaatcga
     1141 tgattccaaa aggaatcccc aatttttccg acgcttctaa agcaactaat tgacctacgc
     1201 gagtaatttt aaacgcagtt ttcttgataa tttcacataa ctctccaaaa tcttttcctt
     1261 tggcattggc aatagcattt ttaacaacac ccggaccact aactccaaca ttgataattg
     1321 cgtctgcttc actaacccca tggaaagccc cagccataaa tggattatcg tcaggtgcat
     1381 tgcataaaac aactaactta gcacaaccaa tagaatcatt ttccttagtt aaatctgcag
     1441 tttcatgaat gatttccccc attaatttta ctgcatccat attaatacca gttttagttg
     1501 atccaacatt taccgaacta caaacattac tagtaacttt cattgcttca ggaattgaac
     1561 gaattaataa ttcttccgcc tttgtcattc ctttggaaac aattgctgta taaccaccga
     1621 tgaaattaac tccagtttca tgagcacatt tatccaatgt tttagcaatt ttaacaaagt
     1681 ccgctgttga tttgcaagca ttagagccaa caaatccaat tggtgttact gaaattcttt
     1741 tattgacaat aggaattcca tatttcattt cgatttgttt acccgtagag actagattct
     1801 tagctacggt agtaattttt ttataaatat tttcacataa aacatcaata tcatgatcca
     1861 tacagtctaa taaactgatc cctaatgtaa ttgttctcac atcaagattt tcttgctcaa
     1921 tcattttatt tgtttctgct acttcaaata aattaatcat tccgattctc cttaaatacg
     1981 atgcatttta ttaaaaatgt tttcatgttg taatttaata tttactccga tttcttcacc
     2041 taatttatct aaatcttcac agatagttcc aaactcttcg gtaattgaac ttagttctac
     2101 aatcatcatc atgttaaaat aaccttgaat gatagtttga gaaatatcca agatattaac
     2161 ttgtttttcc gctaaaaaac tacaaacctt agcaataatt cctacttgat cttttcctac
     2221 tactgtaata atcccctttt gcatactatt acctacctcc taattattta ggatattata
     2281 tcatagttgt atacaatata aaacccctat ttcttgaaat ttcgtcaaaa taaaaacgga
     2341 taaattttat ccgtttttat ttaatcgtaa ggaattatct tcccattaat tttttagcaa
     2401 attcgtaaac agctttacca ttcatcattc cgtaatcttt catttcaatt acatcataag
     2461 gaatatcttt tccagtatct gcaataattt tttcaacttc tttttttgca tatctaactt
     2521 gtgggccaag taatacacaa tctacagtat caataatttt ttcaccttct gtcaaaggta
     2581 aagcaaagat ttctacttct tctccagctt cttttgctgc tacttccatt tttgttacta
     2641 ataaactagt agacatacct gcagcacata ctaacataat tcttgttgcc ataactaact
     2701 ctctccttta ttgttaaatt tcatataaaa agggcatcac aaaaaacgcc cgttttatct
     2761 tttgttattt cagtttaagc tttttgatta cccaatcatt ttcattgcag cttcaaatac
     2821 agctttaccg ttcatagttc cataatctct catatcgatt acatcaacag ggatagctcc
     2881 tgctttacgt ccagcagcca atttttcaat agcagctttt tggaatctaa cttgtgggcc
     2941 aagtaaaata caatctactt cttctaatac tcttggtgca tcagagaatg gtaatgcaaa
     3001 aattttacat tcaacacctg catctttagc agcaccttcc atttttgtta ctagtaagct
     3061 agtagacata cctgctgaac acactaataa aatagttttc ataataatat acactccttt
     3121 ttttttacct aaattaat
//
LOCUS       DAOMTA010000298         3136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1244_length_3136_cov_2.831873, whole genome shotgun sequence.
ACCESSION   DAOMTA010000298 DAOMTA010000000
VERSION     DAOMTA010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_3136_cov_2.831873"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLK68_08580"
     CDS             complement(<1..412)
                     /locus_tag="PLK68_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90497.1"
                     /translation="MENIIDAVSYEENIIQLQLRNVPKHPMIIAKIFTILSECGVNVD
                     MISQVMIEDAMQIEITLDEKYQKNLNDAIMRLKDEVKQLEIATNRKYFKIAVGGKLLE
                     TTPGAAAKVFTILGDNNIHFYQVTTSKRTISFIVD"
     regulatory      complement(473..646)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(893..2227)
                     /locus_tag="PLK68_08585"
     CDS             complement(893..2227)
                     /locus_tag="PLK68_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM90498.1"
                     /translation="MNINIFELGAVLLAGVFFAIIYFLGKVKKYDFGLLTILGLVFGV
                     AVGLIFKGHYLYLEAIGTIYSHLILAIVIPLLLFSIISSITNLGTSIKLKKISAKAII
                     FLLLNTLLASTITLTLAVVTKVGSGINYQLASDYKAVEVPAFIDTIINLFPSNLANSW
                     VNGEVVPIVIFAVIVAIAYNKIARNQQKAVLPFKHFIDAGNRVMGEVVNFVISFTPYA
                     VLALIARAVSKSALSDLIPLLSVLGLAYLLSIIQIFGVTSVLLKVVGKLNPLNFFKGI
                     WPAGVVAFTSQSSIGTIPVTVRQLTKKLGVNEDVASFVASLGANLGMPGCAGIWPVLL
                     AVFAINVLGIDYSIGQYIFLIVLAVVVSIGTVGVPGTATITATALFASAGLPIEIIVL
                     LAPISSIVDMARTATNVVGAASAAMLVARSENELDLAVYNNETIGQPDLEII"
     gene            complement(2454..3002)
                     /locus_tag="PLK68_08590"
     CDS             complement(2454..3002)
                     /locus_tag="PLK68_08590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM90499.1"
                     /translation="MSEFEKMRAGEIYNPRDLKLIYMYDKTARRLHRYNKRCFHVYNM
                     RSRLMKKIINTSGNFWIHPPFQCDYGCNIYLGKDVMINYGCVFLDVCEIKIGDNTLIG
                     PHTQIYTACHSIDPQERLKEIEFGKAVTIGNNVWIGGNCTILPGVTIGDNSVIGAGSV
                     VTKDVPANVLAYGNPCQLKKKI"
BASE COUNT         1066 a          637 c          396 g         1037 t
ORIGIN      
        1 tatcaacaat aaacgagatt gttcgtttag aggttgttac ttgataaaaa tgaatgttgt
       61 tatcccctaa aatagtaaat actttagccg ctgccccagg ggtcgtttct agtaattttc
      121 ccccaactgc aattttaaaa tatttacgat tagtcgcaat ttctaattgc ttaacctcat
      181 cttttaatct cataattgca tcattaagat tcttttggta tttttcatca agtgtaatct
      241 caatttgcat cgcatcttca atcattactt gagaaatcat atctacattt accccacact
      301 cactcaagat tgtaaatatc ttggcaataa tcattgggtg tttaggaaca tttctaagtt
      361 gtaattgaat aatattttct tcgtagctca ctgcatcaat aatattttcc atcattgttc
      421 tcctttacaa aaaaagttgc aatgagtatc attgcaacat aaacagcgta tgaacaatga
      481 tagctcacca ttatctattg ataatgacag tcttatagat cttcactata agcccagagg
      541 ataaacgcat gcaatcattt atctcttcgg cgaacgctcc tcccttttta cctggtttgc
      601 atcttatggt aaaaacttct taacaattcg cgcctctatc taatatctgt ttacaatata
      661 tcacaataga tatcatatta caataccaat tataccgctt taaccagtgt ttttatataa
      721 aaacatgctc atttttggct ttatcaataa tttatatgaa acaaaatcat aaaaatgtca
      781 aaattataca aataaagaaa aataactatt cttgattatt tttccagata gattgttttc
      841 actaaaatat gaacataaaa aagctatgaa tttatcttca tagcttctat ttttaaatta
      901 tttctaaatc cggctgtccg attgtttcat tattataaac agcaaggtct aattcatttt
      961 cacttcgggc aactaacatt gcagcactag cggcaccaac aacattagtt gctgttcttg
     1021 ccatatctac aatacttgaa ataggtgcta ataaaacaat aatttcaatc ggtaagcctg
     1081 ctgaagcaaa taatgcagta gcagtaatgg ttgcagtacc aggcacccca acagttccaa
     1141 ttgatactac tacagctaaa acaattaaaa atatatactg accgattgaa taatcaatcc
     1201 ccaaaacatt tattgcgaag actgctaata aaactggcca aattcctgca catcccggca
     1261 tacctagatt agcccctaaa ctagctacaa atgaagcaac atcttcatta actcctagtt
     1321 tctttgtaag ttgtctaaca gtgacaggaa ttgtcccaat actactttga gaagtgaatg
     1381 ctactacccc agccggccaa attcctttaa agaaattaag cggatttaac tttccaacca
     1441 cctttaataa aacactggtt accccaaaaa tttggattat actcaataaa tatgctaatc
     1501 ctaagacgct caatagcgga atcagatcac ttagagcact cttgcttact gcacgtgcaa
     1561 tcaatgctaa tacagcatac ggtgtaaagc taataacaaa gttaacaacc tctcccatca
     1621 cacgatttcc ggcatctata aaatgcttaa acggtaaaac agctttttgc tgattacggg
     1681 caatcttatt gtaggcaatc gcaacaatta cagcgaaaat tacaataggt actacttcgc
     1741 catttaccca actgttagcc aaattgcttg gaaataaatt aatgatcgta tcgatgaatg
     1801 ccggaacttc caccgcttta taatcacttg ccaattgata atttatgcca cttccaactt
     1861 tagttactac tgctaaggtc agtgttatag tactagctag tagggtgttt aataataaaa
     1921 atattatcgc tttggcactt attttcttta atttgatcga tgttcctaaa tttgtaatac
     1981 ttgaaataat actgaataat aatagcggga taacgatcgc taagatcaaa tgcgaataaa
     2041 tcgttccgat tgcttctaaa tacaaataat gtcctttaaa aatcaatcct acggctaccc
     2101 caaaaactaa tcctaaaata gttaaaagcc caaaatcata cttcttcact ttacctagaa
     2161 aatagataat tgcgaagaaa acacctgcaa gaagcacggc tcctaattca aaaatattta
     2221 tattcattta actgtcctct ttccttaata aatttattta acaacattat taattatgac
     2281 aacagccata cattcgttcc cattcaaaaa tctttttatt ttttaatatc tttacggaaa
     2341 tcatcctatc cctcttttcc tactatatta gtatgatata aatttatact attgctggac
     2401 aaatgtcaag aataatgaaa agttcttagc aaaagcttaa gaactttata aaatcatatc
     2461 ttctttttta gttgacatgg attgccataa gctagtacgt tagcaggaac atctttagtt
     2521 accacactgc ctgctccaat aacgctatta tcgccaatcg taactcctgg taaaatagta
     2581 cagtttccac caatccaaac attattccca atggtaactg ctttcccaaa ttcaatttct
     2641 tttaaacgct cttgtggatc gattgaatga caggcagtat atatttgggt atgtggacca
     2701 atcagagtat tatcaccgat cttaatttca caaacatcta aaaacacaca gccgtaatta
     2761 atcataacat cttttcccaa atagatatta cagccataat cacattgaaa tggcggatga
     2821 atccaaaaat taccgcttgt attaataatt tttttcatta agcggcttcg catattatat
     2881 acatgaaaac accttttatt atacctatgc aaacgccgcg ctgtcttatc atacatatag
     2941 atcagtttta aatctcgggg attataaatt tctcccgctc tcatcttttc aaactcactc
     3001 attttatcac ctacttgtta tcatcataac atataatcta tacctcaatt actagtatat
     3061 aaaattgaag tgaatcccct cgtcagtata atcctctact tatctttaat aatactttta
     3121 ctacactggt atgtga
//
LOCUS       DAOMTA010000299         3134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1246_length_3134_cov_1.994479, whole genome shotgun sequence.
ACCESSION   DAOMTA010000299 DAOMTA010000000
VERSION     DAOMTA010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1246_length_3134_cov_1.994479"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLK68_08595"
     CDS             complement(<1..935)
                     /locus_tag="PLK68_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM90500.1"
                     /translation="MNDERMGNKKILPLLIEFSVPAIIGMLVNAIYNVVDRMFIGNAP
                     QLGAVGIAGITISYPVTLVLMAISLMVGVGGATRFSISLGQKEKDKASIYQGNAIVLT
                     VVFGLMFTVLGNIFITPMLEVLGASKTVLPYASDYLSIVLYGAVFQCVAMCGNNFSRA
                     QGNPKNAMISQLIGAGFNIVFDYILIIQLHMGMSGAAIATIGGQFLSMIWQLCYLCSN
                     RSLIKLDIEHMKLKLIYALDIIKTGIPAFLMQMANSVLNFILNSTLGMYGGDIAISTV
                     GIITSFQTICQMPLTGLMQGQQPLISYNFGADNYHR"
     gene            complement(928..>1220)
                     /locus_tag="PLK68_08600"
     CDS             complement(928..>1220)
                     /locus_tag="PLK68_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM90501.1"
                     /translation="QEELIKELQIDKSAVTRLLQNMENKGLIKRIQSPVDKRFYLLEM
                     TALGQEKQQIVDQTFAKKDFILVSGLSEKEQEELRRMLDVIQTNLKGGKINE"
     assembly_gap    1221..1230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1433..2872)
                     /gene="proS"
                     /locus_tag="PLK68_08605"
     CDS             complement(1433..2872)
                     /gene="proS"
                     /locus_tag="PLK68_08605"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRM90502.1"
                     /translation="MANNKKNDAITAMEDDFAQWYTDVCRKAELMDYSSVKGFIIYRP
                     YSYAMWEAIQQYMDKRFKETGHENVYMPMLIPQSLLQKESDHVEGFAPECAIVTKGGL
                     DDLEENLIIRPTSETLFCEHYAKIVNSYRDLPKLYNQWCSVVRWEKTTRPFLRGSEFL
                     WQEGHTVHASETEARAETLQMLEIYEDTAKNLLAIPMLTGRKTEREKFAGAEETYTIE
                     ALMHDGKALQSGTSHYFGQGFAKAFDMKFLSKENKLEYVYQTSWGVSTRLLGAIIMVH
                     GDNNGLVLPPRVAPTQVMIIPIQQQKAGVLDKAYEIEKQLKEAGIRVKVDASDKSPGW
                     KFSEAEMRGVPLRLEIGPKDIENNACVIAKRNDGTKEKYALDQELINTIETLLDTIHD
                     EMYQKALNHRNSNIRQVNTYDEFKTTLETKGGYIKMMWCGDEACEVKIKEETGATSRC
                     IKEEEEAFGDVCPICGKPAKKVVYFAKAY"
     gene            complement(2926..>3134)
                     /locus_tag="PLK68_08610"
     CDS             complement(2926..>3134)
                     /locus_tag="PLK68_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90503.1"
                     /translation="IELNTLFIDEGFGSLDSQSLDQAIDCLIDIQQDGKLIGIISHVS
                     ELKERIDHKIILSRKNKETKIAIE"
BASE COUNT          953 a          645 c          390 g         1136 t
ORIGIN      
        1 cgatgataat tgtctgcccc aaaattataa ctaatcaatg gttgttgccc ttgcattaaa
       61 cctgttaaag gcatctggca tatagtttgg aaactagtaa tgattcctac tgttgaaatt
      121 gcgatatcac caccatacat tcctaatgta ctattcaaaa taaagtttaa aacactatta
      181 gccatttgca ttaaaaatgc tggtattccc gttttaataa tatctaacgc atagatcagc
      241 tttagtttca tatgttcgat atctaactta atcaaagagc gatttgaaca tagatagcac
      301 agttgccaaa tcatacttaa gaattgtcca ccaattgtcg ctatagctgc accactcatt
      361 cccatatgca actgaatgat taaaatataa tcaaaaacga tattaaatcc ggcccctatt
      421 aattgtgaaa tcattgcatt cttagggttg ccttgtgccc gagaaaaatt attcccacac
      481 attgcaacac actgaaatac tgctccatac aaaacaattg ataagtaatc gctggcatat
      541 ggcaaaactg ttttactagc acctaaaact tctaacatgg gtgttataaa aatattccct
      601 aatacagtaa acatcagccc aaaaacaact gttaaaacaa ttgcattacc ctgatagatt
      661 gaagccttat ctttctcttt ttgtcctaaa gaaatgctga aacgagtagc tccacctact
      721 cctaccatta atgaaattgc cattaatact aatgtaactg gataacttat tgtaattcca
      781 gcaatcccta ccgctcctaa ttgtggcgca tttccaataa acattcgatc aaccacatta
      841 taaattgcat taactagcat cccaataatt gctggaacac taaattctat taataatgga
      901 aggatttttt tatttcccat tctctcatca ttcatttatt ttacctcctt ttagatttgt
      961 ttgtatcaca tctaacattc ttcgaagttc ttcttgttct ttttcactca acccagatac
     1021 taaaataaaa tcttttttag caaatgtctg gtcaactatc tgctgttttt cttgacctaa
     1081 ggctgtcatc tccaataaat aaaaacgttt atcaacaggc gattgaattc gtttgattaa
     1141 ccctttattt tccatatttt ggagcaatct tgttacagca cttttatcaa tttgtaattc
     1201 tttaatcaat tcttcttgat nnnnnnnnnn cattaggaca gaaccttcat tacttgttaa
     1261 tccatactct tttaatcttt gattactatc atttcgattt aatattgcta aatgatgaat
     1321 aatccgtcca attctttcca ttttgcacct ccattagttg atatgtcaac tattatacca
     1381 acattagttg atatgtcaac taacaacaaa aaagatacga aaatttcgta tcttaatatg
     1441 cttttgcaaa atacacaacc tttttagctg gtttaccaca aataggacaa acatcaccaa
     1501 atgcttcttc ctcttcttta atacatcggc tggttgctcc agtttcttct ttgattttaa
     1561 cttcacaagc ctcatcaccg caccacatca ttttgatata accgccttta gtttccaatg
     1621 tagttttaaa ttcatcatat gtgttaactt gacgaatatt tgaattgcga tgatttaatg
     1681 ctttttgata catttcatca tgaattgtgt ctaaaagggt ttcaattgta ttaattagtt
     1741 cttgatctaa tgcatacttc tctttagttc catcattacg ttttgcaatt acacaagcat
     1801 tattttctat atccttagga ccaatttcta atcttaatgg tacgccacgc atctctgctt
     1861 ccgagaactt ccatcctggt gatttatcac ttgcatcaac tttaactctt attccagctt
     1921 cttttagctg tttttcaatt tcataagctt tatctaaaac ccctgctttt tgttgttgaa
     1981 ttggaataat cattacttga gtcggtgcca cccgtggcgg taaaactaaa ccattattat
     2041 caccatgaac catgataatt gctcctaaca atcttgttga aactccccaa gatgtttgat
     2101 aaacatattc taatttattt tcttttgata aaaatttcat atcaaacgct ttagcaaaac
     2161 cttgtccaaa ataatgacta gtgcctgact gtaaagcttt accatcatgc attagtgctt
     2221 caatcgtata agtttcttca gctcctgcaa atttttcacg ttcagttttc cgtcccgtta
     2281 gcattgggat tgctaataag tttttagcag tatcttcata gatttctaac atttgcaatg
     2341 tttcagcccg agcttctgtt tcactcgcat gaacagtatg accttcttgc cataaaaatt
     2401 cagatcctct taaaaatggt cttgtcgttt tttcccaacg tactaccgaa caccattgat
     2461 tatataattt tggtaaatca cgatatgaat taactatttt agcataatgc tcacaaaaaa
     2521 gtgtttctga agtaggacga atgatcaaat tttcttctag atcatctaac ccacctttcg
     2581 ttactattgc gcattctggt gcaaatcctt caacgtgatc agattctttt tgtaataatg
     2641 attgtggaat aagcatcggc atataaacat tctcatgtcc tgtttcttta aaacgtttat
     2701 ccatgtattg ttggattgct tcccacatcg cataactata tggtcgatag ataataaatc
     2761 cttttacgct gctgtaatcc attaattctg ctttacgaca gacatcagta taccactgag
     2821 caaaatcgtc ttccatcgcc gttattgcat catttttctt attatttgcc attatttctc
     2881 ctcatttcat tataatcttt ccctatttta ctattaataa agtgattatt caatagcaat
     2941 ctttgtttct ttgttcttcc gtgaaagaat aattttatga tcaattcgct ctttcaattc
     3001 actaacatgc gaaatgattc caattaattt accatcttgt tgaatatcaa tcaaacagtc
     3061 aattgcttga tctaatgatt gactatccaa agaaccaaag ccctcatcaa taaataaagt
     3121 atttaattct atcc
//
LOCUS       DAOMTA010000300         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1249_length_3132_cov_2.347741, whole genome shotgun sequence.
ACCESSION   DAOMTA010000300 DAOMTA010000000
VERSION     DAOMTA010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_3132_cov_2.347741"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLK68_08615"
     CDS             complement(<1..396)
                     /locus_tag="PLK68_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90504.1"
                     /translation="MNNENKYFIKFLILVGILIAILVGYAGFNFFNISGIFNEKPIYY
                     FCSYHDDYYLDMNDNQIVKLKNDIQNFMVELDYVDEDYDNITFQSVVYEKSKEVVYYF
                     FLVDDEMNTLYAVQFDKRSNSWDKKFVWNG"
     gene            complement(415..1680)
                     /locus_tag="PLK68_08620"
     CDS             complement(415..1680)
                     /locus_tag="PLK68_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRM90505.1"
                     /translation="MNIINNIKKTIAVTLLGGGMPLFIIFICLAILLTSVSVSSDDAY
                     AEREGVQTIELFIQIANNEYETCGNVKGGDKYRKWYTGAADGAPWCATFVSYIAYKAN
                     ILDTAIVKFADCDAGIKWFREKNQFNYTEYYEAGNSYVPQRGDLIFFSSNKNKNDSTR
                     VGIIEECDGSIIKTIEGNSGNAIKKRSYSISNETGTILGYATPNYPSSGSAKLEGALS
                     EAFKFFASNESGNDYNKGFSKGDGYYALGYYQFDSRYDLQEFLRFCYETNPTLYAPFS
                     NFLNVSKSKLRNNKNLEKAWHQVYEADSENFAVMQDTFEYNNYYLPAESGLAGKGIDI
                     SSRCDALKGMCCSLSNWAGTKTAVTIIMDSRINSNMNDKEFVSRVYDYLYSLKYNDYA
                     KYGKTGKKYYNGWHNRWKREKEQCLKYIQ"
     gene            complement(1697..>3132)
                     /locus_tag="PLK68_08625"
     CDS             complement(1697..>3132)
                     /locus_tag="PLK68_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRM90506.1"
                     /translation="DGIHQPLEMFNLTAPICQNYVENYMLETTADTMGYMYPFVYEAL
                     YDSTEEIDKDSKEVLYRYPPVYIGNTKNTNGVVFYDNFVRKGDRTNSNEFIVGTTGMG
                     KTLFLMWLIKERYGLGYKQFIIDIEGKELHRLVHVLGGENINCSDGRSGLINPLQIRL
                     NVPESEKDNEKTPLTEIFPLSEHIRFLRSFFDAYKGNAKEDIRLLHDNLIEKALENIY
                     RGIGITYQTSAQIIVDNYRNKDFPIMLDLYNELYRLLEIANSEKTPNIKEITRIKECI
                     AFVEPMACGADASIFNGHTSIDLNSRLICFNVSGLQDNTNNKVLLTQYFNVLSFIWTS
                     IVSNTENIRQQLYNDEFGVIMDPRCLDIMMYFQTIAKRIRKRKGGLTTATQQISDVLK
                     DSVKDQGEAIISQSAYQFYFGLGSGGIKYFKDTESNLIPESEMEFIQFAEMGDCYFKV
                     GSQTAMRIHITLPDDDLEEFERIKADY"
BASE COUNT         1014 a          630 c          372 g         1116 t
ORIGIN      
        1 accattccac acaaatttct tatcccaact attacttctt ttatcaaatt gaactgcgta
       61 caaggtattc atttcatcat caaccagaaa aaaataatat acaacttcct tgcttttttc
      121 ataaactact gactgaaaag ttatattgtc gtaatcctca tcaacataat ccaactccac
      181 cataaaattt tgaatatcat tttttaactt tactatctga ttatcattca tatctaaata
      241 ataatcatca tgataactgc aaaaataata aatcggtttt tcattaaaaa taccgcttat
      301 attaaaaaaa ttaaaacctg catatccaac taaaattgca attaaaattc ctaccagtat
      361 taaaaatttt ataaaatatt tattttcatt attcatttta tcctccgtcc cttattactg
      421 gatgtatttt aaacactgtt ctttttctct tttccaacga ttatgccatc cattataata
      481 ttttttccca gtctttccgt actttgcata atcattatat tttaatgaat aaagataatc
      541 atacactctt gaaacgaatt ctttatcatt catattacta ttaatcctgc tatccataat
      601 aatagttact gctgtttttg ttccagccca attcgataat gaacagcaca ttccttttaa
      661 agcatcacat cttgaagata tatcgatacc ctttcctgca agtccgcttt cagctggaag
      721 gtaataatta ttgtattcaa atgtatcctg catcactgca aaattttccg aatctgcttc
      781 atatacctga tgccatgctt tttctaaatt cttattattt cttaatttcg attttgaaac
      841 attcaaaaaa ttagaaaacg gcgcatataa agtaggattt gtctcataac aaaatcttaa
      901 aaattcttgt aagtcatagc gactatcaaa ctgataatac cctaacgcat aatatccatc
      961 acccttagaa aagcctttat tataatcatt tcccgactca ttagaagcaa aaaatttaaa
     1021 tgcctctgat agagctcctt caagttttgc cgaacctgaa gatggataat taggagttgc
     1081 atatcctaaa atagtaccag tttcatttga aatgctgtaa cttctcttct taatagcatt
     1141 accactgtta ccctcgattg ttttgattat gctgccatca cattcttcaa tgatgccaac
     1201 acgggtagaa tcatttttat ttttatttga tgaaaagaaa atcaaatctc cacgttgcgg
     1261 tacatatgag tttcctgcct cataatactc tgtataatta aactgatttt tttctctaaa
     1321 ccacttaatg cctgcgtcac agtcagcaaa ttttactatt gcagtatcta aaatatttgc
     1381 tttgtaggcg atgtaagaga cgaatgttgc acaccatggt gcaccatccg cagcacctgt
     1441 ataccatttt cgatacttgt caccgccttt aacattaccg caagtttcat actcattatt
     1501 ggcaatctgt atgaaaagtt caattgtttg cactccttct cgttctgcat aggcatcgtc
     1561 tgatgacact gaaacagaag taagaaggat tgccaaacat ataaaaataa taaaaagagg
     1621 cataccgccc cccagtaatg taaccgcaat agttttttta atattgttaa ttatattcat
     1681 atagttctaa tctgctttaa taatctgctt taattcgttc aaactcttct aaatcatcat
     1741 caggcaaagt aatatgaatt ctcattgctg tttgtgaacc tactttaaaa tagcagtccc
     1801 ccatttcagc aaactgaata aattccattt cactttcagg aatcaaattg ctttccgtat
     1861 ctttaaaata tttaattcca cctgatccta atccgaaata aaactgatat gcagactgag
     1921 aaataattgc ttctccctga tcttttacag agtcctttaa tacatcagaa atctgctgag
     1981 ttgcagttgt aaggccaccc ttacgctttc taattcgctt agcaattgtc tgaaaataca
     2041 tcataatatc taaacagcgc ggatccatta ttacaccaaa ctcatcatta tatagttgct
     2101 gtctaatatt ttcggtgtta gatactatac ttgtccatat aaaagaaagt acattaaaat
     2161 actgagttaa taatactttg ttattggtat tatcctgtaa tccactgaca ttaaaacaaa
     2221 tgagtcggct attcaaatca atactagtgt gaccattgaa tatcgaagca tctgctccac
     2281 atgccatagg ctcaacaaat gcaatacatt ctttaattct ggttatctct tttatgttag
     2341 gagttttctc actatttgct atttccagca gtctgtacag ctcattataa agatcaagca
     2401 ttattggaaa gtctttattt ctataattat ctacaattat ttgggcactg gtttgataag
     2461 ttattccaat tcctctataa atattttcca acgctttttc tatcaaatta tcatgaagaa
     2521 gtctaatatc ctccttggcg tttcctttat aagcatcaaa gaatgaacgt aaaaatctaa
     2581 tatgttctga taatggaaat atttcagtta aaggcgtttt ttcattatcc ttttcacttt
     2641 caggtacatt aagtctaatt tgaagtggat taatcaaacc tgacctgcca tcagaacagt
     2701 ttatattttc cccgcctaaa acatgtacca gcctatgcag ttcttttcct tcaatatcaa
     2761 ttataaactg cttgtatcct aaaccatatc gctctttaat aagccacatc aagaatagag
     2821 tttttcccat accagtcgta ccaacaataa attcattaga attagttcta tcaccttttc
     2881 ttacaaagtt atcataaaat actacaccgt ttgtattttt agtattccct atatatacag
     2941 gagggtatct atataaaact tccttactgt ctttatctat ttcttccgtt gaatcatata
     3001 acgcctcata cacaaaagga tacatatatc ccattgtatc cgctgtagtt tccaacatat
     3061 aattttctac ataattttga caaatcggag cagtcagatt aaacatttcc agtggctggt
     3121 gtataccatc tc
//
LOCUS       DAOMTA010000301         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1250_length_3132_cov_2.098148, whole genome shotgun sequence.
ACCESSION   DAOMTA010000301 DAOMTA010000000
VERSION     DAOMTA010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1250_length_3132_cov_2.098148"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>978)
                     /gene="der"
                     /locus_tag="PLK68_08630"
     CDS             complement(147..>978)
                     /gene="der"
                     /locus_tag="PLK68_08630"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRM90507.1"
                     /translation="IKNLDFVEEEFDEDEIRFSIIGRPNVGKSSLTNAILGEERVIVS
                     NIEGTTRDAIDTAFVKDGQKYRVIDTAGMRKKGKVYENIEKYSILRALSSIEKSDVIV
                     VVIDGNQGVIEQDKHVAGYAHEAGKGVILVVNKWDLVEKDEKTMQKKEKELRSQFKYL
                     DYARIIFLSAKTHQRVQQLFPLIQESFENSHKRVQTSVLNDVLVDAQAINPTTTFNGG
                     RLKIFYANQVSICPPTFVLFVNDPQYMHFSYKRYLENRLRDSFGFEGTPIHIICRKRD
                     "
     assembly_gap    979..992
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<993..1458)
                     /locus_tag="PLK68_08635"
     CDS             complement(<993..1458)
                     /locus_tag="PLK68_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRM90508.1"
                     /translation="MAGVVAIVGRANVGKSTIFNRIVGERISIVEDVAGVTRDRIYAT
                     ATWLTKEFRLIDTGGIELQDASFTAQIKMQAEIAIEEADLIVFVVNGREGITREDEYV
                     ARLLQKTSKPVLLAVNKIDDNAFKDDIYEFYNLGIGDPIAVSGSHGIGIGDLL"
     gene            complement(1460..2128)
                     /gene="cmk"
                     /locus_tag="PLK68_08640"
     CDS             complement(1460..2128)
                     /gene="cmk"
                     /locus_tag="PLK68_08640"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRM90509.1"
                     /translation="MKKISIAVDGPSAAGKSSIAKIVAKRLDYIYIDTGAMYRCVGYY
                     CLENNIDLKDEQAVSQALKQIKIEMDSNNHIFLNGQDVSQVIRQDQVSMSASVVSSYQ
                     AVRTFLVEQQRQMANVGGVILDGRDIGTVVLPNAELKIYQIASVDTRAKRRYLENQER
                     GLTADLELIKKDIEQRDYQDMNRDISPLKQAEDAIVLDTSAMTLQEVVDAVLKLVEEK
                     IKEV"
     gene            complement(2171..>2780)
                     /locus_tag="PLK68_08645"
     CDS             complement(2171..>2780)
                     /locus_tag="PLK68_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90510.1"
                     /translation="VVHIDNLDNINTSEFSKGYFVNQEESVVTKAEEEKKAAKKKMTL
                     MERLASMSPEEDAKKAKLVMDEVQEEAEQKENELEEALKKDTGVVSSGTAEDTLEDEE
                     LAKIEQTRSLEEMLRQIKEKDQREVEKVLKQKEDTADLKVIKNQTNDKTIDSKSKAKQ
                     SRSDIVDEKKSDRIATILNYVIIFLVVVFIGLCGMIGYQLFF"
     assembly_gap    2781..2790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          932 a          679 c          380 g         1117 t
ORIGIN      
        1 tcgttatctc attttgatta tcgatcaaaa ctctgcttaa agctgttgcc caacttccag
       61 ttcctagtac cgcaatttta ctcattgtta cacttcctta tattattgta tcaatattat
      121 accaaactaa ttaattaata gcacgactaa tcacgtttac gacaaataat atgaattggc
      181 gttccttcaa aaccaaaaga atctcttaaa cgattttcta aataacgctt ataactaaaa
      241 tgcatgtatt gaggatcatt aacaaataga acaaaagttg ggggacaaat actaacctga
      301 ttagcataga agatttttaa gcgtccacca ttgaacgtag ttgttggatt gattgcttga
      361 gcatcaacaa gaacatcatt taaaacacta gtttgaacac gtttatgtga attttcaaat
      421 gactcttgaa tcaatgggaa taattgttga actcgttgat gagttttggc agataagaaa
      481 ataatccgcg cataatccaa atatttaaac tgactacgca attctttttc tttcttttgc
      541 attgtcttct catctttttc aactaaatcc catttgttaa caactaaaat tactccctta
      601 ccagcttcat gagcataacc ggcaacatgt ttatcttgtt caataactcc ctgattccca
      661 tcaataacaa caacgataac atcacttttt tcaattgatg ataaggctct caaaattgaa
      721 tatttttcaa tattttcata taccttacct ttttttcgca ttccggcagt atcaataaca
      781 cgatattttt gtccatcctt aacaaatgct gtatcaatcg catcacgagt tgtcccttca
      841 atattcgaaa caattactct ttcttcacca agaatcgcat tcgtcaacga tgattttccg
      901 acatttggtc gtccaataat tgagaaacga atttcatctt catcaaattc ttcttcaaca
      961 aaatcgagat tcttaatgnn nnnnnnnnnn nnctaataag tcaccaattc caattccatg
     1021 acttcctgat actgcaattg gatcaccaat tcctaaatta tagaattcat aaatatcatc
     1081 tttaaaagca ttatcatcaa ttttgttaac agccaataaa accggcttac ttgttttttg
     1141 taaaagacgt gctacatact catcttcacg agttattcct tcacgtccat taacaacaaa
     1201 aacaattaaa tctgcttctt caatggcaat ttctgcctgc atcttgattt gggcagtaaa
     1261 agatgcatct tgtaattcaa ttcctcccgt atcaatcaaa cggaattctt ttgtcaacca
     1321 tgttgcagtt gcataaatac gatcacgagt aacacccgca acatcttcta caatggaaat
     1381 acgctctcca acaattcggt taaaaatagt tgatttacca acattcgctc gtccaactat
     1441 tgctactaca cctgccatat tatacctcct ttattttttc ttctactaat tttaaaacag
     1501 catcaacaac ttcttgtaaa gtcatcgctg atgtatctaa tacgatggca tcttctgcct
     1561 gttttaacgg tgaaatatcg cgattcatat cttgataatc acgttgttca atatcttttt
     1621 tgatcagctc taaatctgct gttaatcctc gctcttgatt ttctaaataa cgacgtttag
     1681 cacgggtatc aacactagca atttgatata ttttcaattc tgcatttggc aaaacaactg
     1741 tcccaatatc acgcccatct aaaatcacac caccaacatt tgccatctga cgctgctgtt
     1801 ctacaagaaa cgtacgaacc gcctgataac ttgatactac actagcagac atagaaactt
     1861 gatcttgacg gatcacttgg ctaacatctt gcccatttaa aaagatatgg ttattactat
     1921 ccatttcaat tttgatttgt ttcaaagcct gagataccgc ttgttcatct tttaaatcaa
     1981 tattattttc aagacaataa tatcctacac aacgatacat tgcccctgta tcaatataga
     2041 tataatctaa tcgtttagca actatttttg caatactgct tttaccagcc gcacttggcc
     2101 cgtcaacggc aatagaaatt tttttcataa tatcctccac tccttgaaaa aaactaaggg
     2161 taaccttagt ttaaaagaac aactggtatc ctatcatacc acacaatcca ataaatacaa
     2221 ctactaggaa tataattaca tagtttaaga tagtagcgat tcgatcactt ttcttttcat
     2281 caacaatatc actacgagat tgttttgcct ttgatttact atctatagtt ttatcatttg
     2341 tttgattttt aatcactttt aaatctgctg tgtcttcttt ttgtttaagt actttttcaa
     2401 cctctcgctg atctttctct ttaatctgtc gaagcatttc ctctaacgaa cgtgtttgtt
     2461 caatttttgc caattcttca tcttctaaag tgtcctcagc tgttccagaa ctcacaacac
     2521 ctgtatcttt tttcaatgct tcttcaagtt cattttcttt ttgctctgct tcttcctgaa
     2581 cttcgtccat taccagctta gcttttttag catcttcttc tggtgacatg ctggcaagtc
     2641 gttccattaa agtcatcttc tttttagcag cctttttttc ttcttcagct tttgtaacaa
     2701 ccgattcctc ctgatttaca aaataccctt tagaaaattc tgaagtattg atattatcca
     2761 gattatcgat atgaactact nnnnnnnnnn tttaaaatat ccattcttgt attgtactgc
     2821 gcttctttac caacactgac cttagcactt cgcaaagctc ggtttatttc ttcactgctt
     2881 tcttgacgca tttgttcaaa agttttagct tctgtcaaag tgtcattaat atctggcaag
     2941 tctttttctt cttgtttaaa agaacgattt attgtcgcta aaaaatcatc atcttcaata
     3001 tcatcaatga tatcaattgt atcctctata ttttcacgat taatccctgc ttcatctttt
     3061 aaaccagctc gtaaatcgcg atatttgttt acacgtgaac tattccctcg aaatcaccct
     3121 ttcgtcgtac at
//
LOCUS       DAOMTA010000302         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1263_length_3117_cov_2.632920, whole genome shotgun sequence.
ACCESSION   DAOMTA010000302 DAOMTA010000000
VERSION     DAOMTA010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1263_length_3117_cov_2.632920"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLK68_08650"
     CDS             complement(<1..219)
                     /locus_tag="PLK68_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90511.1"
                     /translation="MKKRAYVKNQIQIIKKTKARFLSIFCIVFLGAAFFAGLRHTASI
                     MEITMDSYLQEHAFNDLNYVATLGFTNED"
     gene            complement(221..925)
                     /locus_tag="PLK68_08655"
     CDS             complement(221..925)
                     /locus_tag="PLK68_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90512.1"
                     /translation="MKSFITFNNVKKTYKVGDVEINASDGVDFEVNKGEFVVIVGPSG
                     AGKTTILNLLGGMDKATSGQILVDGQDVAKYSERQLTQYRRDDIGFVFQFYNLVQNLT
                     ALENVELATQISKNPLDVRMVLERVGLNKRLDNFPAQLSGGEQQRVAIARAIAKNPKL
                     LLCDEPTGALDYQTGKAILGLLREMCDKYTMTVIVITHNSALAPMADRIIHLKNGQVA
                     SMNINEHPKSIAEIEW"
     gene            1098..>1259
                     /locus_tag="PLK68_08660"
     CDS             1098..>1259
                     /locus_tag="PLK68_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM90513.1"
                     /translation="MILSDSHSVSKTDLLTLLKNNSVNYYIHCGDIYMTYDGINLNSF
                     YLVRGNNDFG"
     assembly_gap    1260..1269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1270..1675
                     /locus_tag="PLK68_08665"
     CDS             <1270..1675
                     /locus_tag="PLK68_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRM90514.1"
                     /translation="SEIEIKYAILNGNIPDELFITIDDLKFYIVHGHRYDVDYNLDYL
                     THTAKEKGADIVCFGHTHRPYYDFHEGITFINPGSVCYPRGQYRNPTYCIFDTKTKKS
                     TFYDVTTLEPCDPFSPMERPKRKEPFYKKWFK"
     gene            complement(1668..2930)
                     /locus_tag="PLK68_08670"
     CDS             complement(1668..2930)
                     /locus_tag="PLK68_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90515.1"
                     /translation="MGLIKLAIRSLKTDFLKSLFYFLSFILTTIFIFLFFNLTLNPST
                     GINLGGNDAKLITTIAAFVILIAMVCVFMANDFYVLAKTKDVSIVLMSGASVYQVGIY
                     LLLQSTIIMFLAIPIGFVISYPLVPLVNNLLFMVFDYQGSLNYISSNTFMATAIILFC
                     EIGWCTLLNMGYCYRTSINKMVTANVKIEKFGIGVKKLSNRIYLVLFILPMIVFPFLN
                     DTASHLLIALIGIIGVYGLVKNIIPEFIENRQTNESLEDRCLLITLGNVRYDLEKVRM
                     LVLVITIAAIILMCTTIYTLDTPLISMVTLMSYFSVMVLLSITTIFKVGMELQGRKRS
                     FLNLYHMGYDLKDLKKIIDLEMIIFYGLIIVIPLLYQIIILIKLYSLGLINFYLVGGL
                     LLIQIIPMLVCMIICTLMYQKVLPEPII"
     gene            complement(2930..>3117)
                     /locus_tag="PLK68_08675"
     CDS             complement(2930..>3117)
                     /locus_tag="PLK68_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HRM90516.1"
                     /translation="NDGVSILMVTHDSKIASYSSKLLYIKDGVIDETIERKDMSQKEY
                     FYKIVDINSTESQALFD"
BASE COUNT         1136 a          564 c          377 g         1030 t
ORIGIN      
        1 atcctcattt gtaaatccta gtgttgcaac gtaattaaga tcattgaaag cgtgttcttg
       61 aagataacta tccatcgtta tttccataat tgaagccgta tgccggagtc ctgcaaaaaa
      121 ggcagcacct aaaaagacaa tacaaaaaat cgacaaaaaa cgtgctttag ttttttttat
      181 tatttgaatt tgatttttta catatgctct ctttttcata ttaccactca atttccgcga
      241 ttgacttagg atgttcgttt atattcattg aagctacttg accattttta agatgaatga
      301 ttcgatctgc cattggagct aaagcactat tatgagtaat aacaattact gtcattgtat
      361 atttatcgca catctcccgt agtaatccta aaatcgcttt accggtttga taatctaatg
      421 ctcctgtcgg ttcatcacat aaaagcagtt taggattttt agcaattgct cgcgcaatag
      481 ccacccgctg ctgctcacca cctgataact gagccggaaa attatctaac cgtttattta
      541 atcctacacg ttccaaaacc attctcacat ctaaaggatt cttcgatatc tgggtagcta
      601 attcaacatt ttctaaagcc gtcagatttt gaaccaaatt ataaaattga aaaacaaaac
      661 caatgtcatc tcgtcgatat tgcgtcaact gtctttcact atatttagca acatcttgac
      721 catcaactaa aatttgccca cttgttgctt tatccattcc ccctaaaaga ttcaaaatag
      781 ttgtttttcc agcaccagaa ggcccaacaa ttactacaaa ttcaccttta ttaacttcaa
      841 aatcaacacc atctgatgca ttgatttcga catcaccaac tttatatgtc tttttaacat
      901 tattaaatgt aatgaaactt ttcatttatc atcactacct ttctaattaa aacatataca
      961 taattatatc acgaaactat tctcaatcca atttctactt ttttaataaa taaattaaat
     1021 aatgctactc atattacaag aatttttata aaaatcgtgc taaaattaaa tcaattagga
     1081 ggtctattat gaaaataatg attttatcag attctcattc tgtttctaaa accgatttat
     1141 taaccctttt aaaaaacaac tctgttaact attacattca ctgcggtgat atttatatga
     1201 cttatgatgg catcaatctt aatagttttt atttagtccg tggtaataac gattttggtn
     1261 nnnnnnnnnt tcagaaatag aaataaaata tgctattctt aatggtaata ttcctgatga
     1321 attatttatt acaatcgatg atttaaaatt ttatattgtc catggtcatc gctatgatgt
     1381 tgattataac ctagattatt taacccatac cgcaaaagaa aagggagctg atatagtttg
     1441 tttcgggcac actcatcgac cttattacga ttttcatgaa ggaatcactt ttattaatcc
     1501 tggtagtgtt tgttatccta gaggtcagta tcgtaatcca acttattgta tttttgatac
     1561 taagacaaaa aaaagtactt tttatgatgt cacgacatta gaaccatgtg accctttttc
     1621 cccaatggaa cgtcctaaaa gaaaagaacc attttataaa aaatggttca aataatcggt
     1681 tccggcaaaa ctttttggta catgagcgta caaataatca tacaaactag cattgggata
     1741 atttgaatca ataacaatcc ccccactaga taaaaattaa tcaatcctaa actatacaat
     1801 ttaattaaaa taattatttg atacaacaaa ggaataacaa taattaaacc atagaagata
     1861 atcatttcca aatcgattat cttttttaaa tcttttagat cataccccat atgatacaag
     1921 ttaagaaagc tcctttttcg tccttgcaat tccattccta ctttaaaaat agttgtaatc
     1981 gataacaata ccatgacact aaaatatgac atcaacgtaa ccatactaat caatggggta
     2041 tctaaagtat aaatagttgt acacatcaaa ataatcgctg ctatggttat tactaaaaca
     2101 agcattctaa ctttttctag atcatagcga acatttccca gcgtaattaa taaacagcgg
     2161 tcttctaacg attcattagt ttgccgattt tcaatgaatt caggaattat attcttaact
     2221 aggccataaa caccaattat tccaataaga gcaatcaata aatgactagc tgtatcatta
     2281 agaaaaggaa atacgatcat tggtaaaata aacaaaacaa gatatattcg atttgatagt
     2341 ttctttacgc ctattccaaa tttttcaatt ttaacatttg cagtcaccat cttattgatg
     2401 ctggtacgat aacaatatcc catatttaaa agcgtacacc agccaatttc acaaaataaa
     2461 atgatagcag ttgccataaa agtattactc gaaatataat ttaaacttcc ttgataatca
     2521 aagaccataa aaagtaaatt atttactaaa ggaactagag gataactaat gacaaaacca
     2581 attggaatag ctaaaaacat gataatcgta ctctgtaaaa gcaaataaat cccaacttga
     2641 taaacagatg cccctgacat caaaacaatt gacacatctt tagttttcgc taaaacataa
     2701 aaatcattag ccataaatac acaaaccatt gcaatcaaaa ttacaaaagc agctatcgta
     2761 gtaatcaact ttgcatcatt cccaccaaga ttgatccctg tactaggatt aagtgtaaga
     2821 ttaaagaata aaaagataaa aatcgttgtt aaaataaaag atagaaaata aaataaactc
     2881 tttaaaaaat cagtttttaa agatcttatt gctaatttta ttaagcccat tagtcaaaca
     2941 acgcttgcga ttctgtcgaa ttaatatcaa cgattttgta aaaatactct ttttgactca
     3001 tatcttttcg ctctattgtt tcatcaatca caccatcttt aatatataat aatttcgatg
     3061 aatacgaagc aattttagaa tcatgagtta ccattaaaat agatacaccg tcattaa
//
LOCUS       DAOMTA010000303         3115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1267_length_3115_cov_2.647712, whole genome shotgun sequence.
ACCESSION   DAOMTA010000303 DAOMTA010000000
VERSION     DAOMTA010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_3115_cov_2.647712"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(20..132)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(202..759)
                     /locus_tag="PLK68_08680"
     CDS             complement(202..759)
                     /locus_tag="PLK68_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRM90517.1"
                     /translation="MKCKKCGQELEIGVKFCPNCGEDCVSQDTGIPVKESVDKTKSAI
                     NDFGENMQKKEVEISGKKFNMLEVLTFGSGILAIISCFLPFASVSIFGYSQSVSLMDG
                     GDGIICIALVVVALILSYLHKDIVALGLAGATAVLAVYEIFNAKSVLGGYGNISIGAY
                     LLLLAAIGLVAGIFLVYNNTKKQQN"
     gene            complement(886..2085)
                     /locus_tag="PLK68_08685"
     CDS             complement(886..2085)
                     /locus_tag="PLK68_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM90518.1"
                     /translation="MEERNPMEEQVEKEENSNQPESRMGKHNKRKVTVNTNGLFKIVI
                     TLLVVASLGMNVFLFTKVNKSSSSGSSSNKNATVENVNYDVKSSTTDVIEKVNSSVVG
                     VLVYANGTASGSGSGVVYRVDGKTAYIITNAHVVSGATDVQVVFSNKESVNATIVGSD
                     TYSDIAVLKLTADFDMTAIKCGDSSLLDQGETVLAIGSPLGIEYAGTVTQGIVSGIDR
                     TVSVDLNDDGQEDWDMNVIQTDAAINPGNSGGALVNMAGELVGITSMKLSNTSVEGMG
                     FALPINDVITSVEQIIENGKVTRPQIGISGVSLSGYSSYQLRYYRINTDLTDGIYVSR
                     VTSGGAASKAGIQEGDIIVKFDGKEVTTYKSFLTELYSKEPGDKVSVVVNRNGTEKTI
                     EVTLGEQ"
     gene            complement(2193..>3115)
                     /locus_tag="PLK68_08690"
     CDS             complement(2193..>3115)
                     /locus_tag="PLK68_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM90519.1"
                     /translation="TNQSFRDAFKIKHLRGKYYKDIFTDQLLNIVDQSYIFERKFSTA
                     VCIDERYYQIETTPVFRDDVAFDGSIILFTDVTQVKEIEKMQKQFFSDISHELKTPMS
                     AIIGSVEILQKEGIKNKDTFNEFMGILLKESYRMQNIIGDILELSRLEQPQATLSPAL
                     VDIDSLIKDTVELFEPLAKDKQLSLVYQTKIKEELMLDYTTVKTILNNLVSNAIKYSN
                     AGVISIKCNYKDDNLIIVVQDEGVGISKDNIPFIFDRFFQVDRSRSKKLGTGLGLSIV
                     KKMVELNNGTIDVESTPGIGTTFTVTLPIL"
BASE COUNT         1018 a          658 c          357 g         1082 t
ORIGIN      
        1 gcattaatta ttaactaaaa aatgtcattc ttccatccag actatactgt cggtgtagga
       61 atttcaccta ctcagcttat gctcgcggac tttaccgccg gtcgggaatc acaccctgcc
      121 ctgaagactt tcttttccta tataatatag catagtattc ctactatttc aataagaaaa
      181 aactaacagt tagttattct gtcagttttg ttgttttttt gtattattat aaactaaaaa
      241 gattcctgca accaatccaa tagcagccaa gagcaataaa tatgctccga tagaaatatt
      301 cccataacca cccagtactg atttagcatt aaaaatttca tataccgcca aaactgcagt
      361 tgcaccagct aagcctaacg ctacaatgtc tttatgaaga taggataaaa tcaatgcaac
      421 aacaactaaa gcaatgcaaa taataccatc tccaccatcc attaaactaa cactttgact
      481 atatccaaaa atcgatactg aagcaaatgg taaaaagcat gaaattattg ccaagatacc
      541 cgacccaaac gtaagtacct cgagcatgtt gaattttttc cctgaaattt caacttcttt
      601 tttctgcata ttttccccaa aatcattaat tgcacttttg gttttatcaa cagactcttt
      661 taccggtatt ccagtatcct gacttacaca atcctcaccg caattaggac aaaatttcac
      721 ccctatttct aattcctgac cacatttttt acatttcata attttccctc atttcatttt
      781 taagttacct ttccctaatt ataacttata atcacattat attataaatc tccattttcc
      841 acaaataaaa aacaccagct ctttattagc tggtgtcagt tattcttact gttcacccaa
      901 tgtcacttct attgtttttt cagtaccatt acggttaacc acaacagaaa ctttatcccc
      961 aggttctttt gaatataatt cagttaagaa actcttataa gtagttactt ctttaccgtc
     1021 aaacttaaca ataatatctc cttcttgaat acccgcctta ctagctgctc ctccagaagt
     1081 aactcgagat acataaatac catctgtcaa atcagtatta atgcgataat agcgtaattg
     1141 atagcttgaa taccctgata aagatactcc tgagatacca atttgtggac gtgtcacttt
     1201 accattttca ataatttgtt caactgaagt aataacatca ttgattggta aagcaaatcc
     1261 cattccttct actgaagtat tagataattt cattgaagta atcccgacta attcaccagc
     1321 catatttacc aaagcaccac cactgttacc agggttgatt gctgcatcag tttgaataac
     1381 attcatatcc caatcttctt gaccatcatc atttaaatcc actgaaacag tacgatcaat
     1441 tccacttaca attccttgag taactgtccc tgcgtattca attccaagcg gactaccaat
     1501 cgctaataca gtttctccct gatccaataa actagaatct ccacatttaa tagcggtcat
     1561 atcaaaatca gcagttaatt ttagtactgc aatgtcactg taagtatcac taccaacaat
     1621 cgttgcattt acagattctt tatttgaaaa tactacttgc acatcagtag ctccacttac
     1681 tacatgggca ttcgtaataa tatatgctgt ttttccatca acacgataaa caactccact
     1741 accgctacca ctagcagttc cattagcata gactaatact ccaacaacac tactattcac
     1801 tttttcaatc acatcagttg tagagctctt cacatcataa tttacatttt ccacagttgc
     1861 attcttattc gatgaagaac cacttgacga gctcttatta acttttgtaa ataagaaaac
     1921 attcattcct aaagaggcaa ccactaaaag agtaataact atcttaaata aaccatttgt
     1981 atttacagtc acttttcttt tattatgttt tcccattctt gattcaggtt ggttactatt
     2041 ttcttctttt tctacttgtt cttccattgg atttctctct tccatttata ccactccttt
     2101 tactttacac acatatctta aataaataat ttgttcaaat tatttttata ttgtgtgaaa
     2161 aattgtgtta cttgtaaaag aaattatatt ttctacaaaa ttggcaaagt taccgtgaaa
     2221 gtagtcccaa ttccaggagt gctttctaca tcaatggtcc cattatttaa ttccaccatt
     2281 tttttaacaa tactcaatcc taaaccggtc ccaagtttct tacttctaga acggtccact
     2341 tgaaaaaagc gatcaaagat aaacgggata ttatctttag aaatgcctac cccttcatcc
     2401 tgaacaacaa ttattaaatt atcatcttta taattacatt taattgaaat aaccccagca
     2461 ttagaatatt taatagcatt agaaaccagg ttatttaaaa tcgttttaac ggtcgtataa
     2521 tctaacatca attcttcttt gatttttgtt tgatatacta atgacaattg tttatctttt
     2581 gctaaaggct caaataattc cactgtatct ttgatcagtg aatcaatatc taccaatgca
     2641 ggacttagag tcgcttgagg ctgttctaat cgagataatt ctaaaatatc tccaataata
     2701 ttctgcatcc gataactttc ctttagtagt atacccataa attcgttaaa agtatcttta
     2761 ttttttattc cttctttttg aagaatttct acgctaccaa taattgcact cattggtgtt
     2821 tttaattcat gagaaatatc tgaaaaaaac tgtttttgca tcttttcgat ttcttttacc
     2881 tgtgtaacat cagtaaaaag aataatactt ccatcgaatg ctacatcatc acgaaataca
     2941 ggtgttgttt caatttgata ataacgttca tcaatacaaa ctgcagttga aaactttctt
     3001 tcaaaaatat aactttgatc aacaatattt aataactgat ctgtaaaaat atctttataa
     3061 tatttacctc gcaaatgttt gattttaaaa gcatcacgga aactctgatt agtga
//
LOCUS       DAOMTA010000304         3114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1269_length_3114_cov_2.334750, whole genome shotgun sequence.
ACCESSION   DAOMTA010000304 DAOMTA010000000
VERSION     DAOMTA010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_3114_cov_2.334750"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLK68_08695"
     CDS             <1..742
                     /locus_tag="PLK68_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM90520.1"
                     /translation="GVKTLCLALIRNSNRVHIVYRLYDENNSILENNEIIKPTLSIQD
                     YYDVIDSMLAKYVDIKVIGLSTPGIIRGDQIISMSIKGLEKISITHDFTLKYSQKFVF
                     SNDANTIALGYYSSKENCSSLSFVFQPVSSNSGAGHIVNDRLIAGHHHVAGEIQYLPL
                     SYSKDVLELHQTPEGAIEAMAKTVTSIISFLDPQIIVICCFLITNIEELKKEVAKYIP
                     KDYIPEIVLIDYLQEYMLLGTLLLCHQD"
     gene            1017..2285
                     /locus_tag="PLK68_08700"
     CDS             1017..2285
                     /locus_tag="PLK68_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HRM90521.1"
                     /translation="MNKKVTIREAVNYVINSKGEKHTLLGIGPMSENFLRASLEISKE
                     KDFPLMYIASRNQVDAYKFGGGYVFNSDQKLFKEKIEEIAQEINYDNVYYLCRDHGGP
                     WQRDKERADHLPEDEAMAIAKESYKEDILNGFDLLHIDPTKDPDEYCKVVDIDVVFNR
                     TIELIEYCEQVRKEYGIEREIAYEVGTEETSGGLTSMDRYENFIQRIESYTQEHDLPM
                     PIFIVGQTGTLTRLTKNVGNFSYENSLELSAISTKYGVGLKEHNGDYLSEAKLLAHLP
                     LNITAMNVAPAFGTIETMALLELIEVEKQFAKFNMIKEPSNLENVIKYESIHSMKWKK
                     WLTDEVDMSDLDNLDEQTTLQITELCGHYTYSKPKVALEINKLYENLASIKIDGKRFV
                     IEKLKEEMQKHVRCFNMEGLTSKMLACKKG"
     gene            2297..2758
                     /locus_tag="PLK68_08705"
     CDS             2297..2758
                     /locus_tag="PLK68_08705"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HRM90522.1"
                     /translation="MQEKAIHKEMVFLKQALSTKDDVIELLSDQAIKLDLINSKKDFK
                     EAVYKREEMASTSIGYQIAIPHGISHTVNRAFIGFVQIKDAFHWNHDEKQSVQLIFLI
                     GVPQDNQNNIHLKFISLLSRRLLDDNFRKQLIEVEDVEDAYRALNAINEQL"
     gene            2771..3085
                     /locus_tag="PLK68_08710"
     CDS             2771..3085
                     /locus_tag="PLK68_08710"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HRM90523.1"
                     /translation="MKIVAVTACPTGIAHTYMAQEAIEKECRNRGYEVKVETQGSMGI
                     ENELEQEEIDEADAVILAIAVGIDGEERFEEKQDAGKVIQVEPGDVIKDAVRVIDQAE
                     NL"
BASE COUNT         1182 a          344 c          576 g         1012 t
ORIGIN      
        1 tggtgttaaa acattgtgtc ttgctttaat taggaatagt aatcgtgttc atattgtgta
       61 ccgtttatat gatgaaaata atagtatttt agaaaataat gaaattatca aacctacttt
      121 atcaatccaa gattattatg atgtaatcga tagcatgctg gctaaatatg tagatattaa
      181 agttattgga ttatcgactc cgggaattat acgtggtgat cagattatct caatgagtat
      241 aaaggggcta gagaaaattt caattactca tgattttact ttgaaatatt cgcaaaaatt
      301 tgtttttagt aatgatgcta atacaattgc tttaggctat tatagttcta aagaaaactg
      361 ttcatcactg tcatttgttt ttcaaccggt tagttcgaac tcaggggcag gacatattgt
      421 aaatgatcgt ttgattgctg gacaccatca tgtagctgga gaaatacaat atttgccatt
      481 atcatattca aaagatgttt tagaattaca tcaaacacca gaaggagcca ttgaagcaat
      541 ggctaaaact gttacatcaa taatcagttt tttagatccg caaattattg ttatatgttg
      601 ttttttaatt actaatattg aagaattgaa aaaagaggta gctaaatata ttcctaaaga
      661 ttatattcct gaaattgttt taattgatta tctccaagaa tatatgttat taggtacgct
      721 attattatgt catcaagatt aaaagatagt agttttatat gaagaaaaat taattattta
      781 ttattagcag cgatgctgat ttaaaaaaat agaatttatg ctctagttga gtttaaattc
      841 tattttttct taatcaacaa tagggtgcaa tttttgtaac tttttctttc atttgtttta
      901 tcttatgtca ttgtgactaa ttgaatatga tatatgtgta gatagaaaca aatacctatc
      961 tcataaatca aaagtcgaca tttgattaat tgctaaatga tgaggagaag ataaaaatga
     1021 ataaaaaagt tacaattaga gaagcagtta attatgtaat taattcaaaa ggagaaaaac
     1081 atacattact tgggattgga cctatgtctg aaaatttttt acgggcatct ttagaaattt
     1141 caaaagaaaa agattttcca ttaatgtata ttgcaagtcg taatcaagtt gatgcatata
     1201 aatttggtgg tggatatgta ttcaacagtg atcaaaagtt attcaaagaa aaaatcgaag
     1261 agattgcgca agaaattaat tatgataatg tctattattt atgccgtgat catggtggac
     1321 catggcaaag agataaagaa agagcagatc atttaccgga agatgaagct atggcaattg
     1381 ccaaagagtc atataaagaa gatattttaa atggttttga tttattacat attgatccaa
     1441 ctaaggatcc tgatgaatat tgtaaagtag tggatataga tgttgtcttt aatcgtacta
     1501 tagaattaat tgagtattgt gagcaagtta gaaaagaata tggaattgaa cgtgaaattg
     1561 catatgaagt agggacagaa gaaacaagtg gtggtttaac ttcgatggat cgctatgaaa
     1621 attttatcca aagaattgaa tcttatactc aagaacatga tttaccaatg ccaattttta
     1681 tcgttggtca aactggaacg ttaacacgtt taactaaaaa tgttgggaat ttcagttatg
     1741 aaaattcttt agaattaagt gcaatatcaa ctaaatatgg agtaggatta aaggaacata
     1801 atggtgatta tttaagtgaa gctaaattac tagcacattt acctttaaat atcacagcaa
     1861 tgaatgttgc tccagctttt ggaacgattg aaacgatggc tttattagag ttaattgaag
     1921 tagaaaaaca gtttgctaaa tttaatatga ttaaagagcc atcaaattta gaaaatgtta
     1981 ttaagtatga atcaattcat tcaatgaaat ggaaaaaatg gcttactgat gaagtagata
     2041 tgtcggattt agataattta gatgagcaaa caacattaca aattacagaa ttatgcggtc
     2101 attatactta ttctaaacct aaagtagcat tagaaattaa taaattatat gaaaacttag
     2161 caagcattaa aattgatgga aaaagatttg ttattgaaaa actaaaagaa gaaatgcaaa
     2221 aacatgttag atgttttaat atggaaggat taacatcaaa gatgcttgct tgtaaaaaag
     2281 gataagggga atgattatgc aggaaaaagc aattcataag gaaatggtct tcttaaagca
     2341 agctttatcg actaaagatg atgttattga attattatca gatcaggcta ttaaactaga
     2401 tttaattaat agtaaaaaag attttaaaga agcggtttat aaacgagaag aaatggcttc
     2461 aacttcaatt ggttaccaaa ttgcaattcc tcatggaatt agtcatactg ttaatcgtgc
     2521 ttttattgga tttgttcaaa taaaggatgc atttcattgg aatcatgatg agaaacaatc
     2581 agtccaattg attttcctta taggcgtgcc ccaagataat caaaataata ttcatcttaa
     2641 atttatttct ttattaagta gaagactttt agatgataat tttagaaagc aattgattga
     2701 agtagaagat gttgaagatg catatcgtgc attaaatgca atcaatgaac aattatagga
     2761 ggaaatgaat atgaaaattg ttgcagttac agcatgccca accggtattg cgcatacgta
     2821 catggctcaa gaagcaattg aaaaagaatg tcgtaatcgt ggttacgaag taaaagtaga
     2881 aactcaagga agtatgggga ttgaaaacga attagaacaa gaggaaattg atgaagcgga
     2941 tgctgttatt ttggcaattg cagtggggat cgatggtgaa gaacgttttg aagaaaaaca
     3001 agatgctggt aaagtaattc aagtcgaacc tggggatgta attaaagatg ccgttagggt
     3061 tatagatcaa gcagaaaact tataggagaa gaagttatga aaaaaagaaa ttgg
//
LOCUS       DAOMTA010000305         3114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1270_length_3114_cov_2.182086, whole genome shotgun sequence.
ACCESSION   DAOMTA010000305 DAOMTA010000000
VERSION     DAOMTA010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_3114_cov_2.182086"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1297..2748)
                     /locus_tag="PLK68_08715"
     CDS             complement(1297..2748)
                     /locus_tag="PLK68_08715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM90524.1"
                     /translation="MLLTEKYKKQIYYQFPKDFLWGVAMSAKQAEGADDRALTVADLQ
                     NYDPNDKAKVKGDLSKAEILDRLNYPERYNFPKKIGIEFYHRYKEDLILLKEMGITCF
                     RFSISWARVFPNGIDGEPSEQGLKYYDKIFNLLKEMGIVPIVTLYHDDMPIDLALKYN
                     GFLNEKVVTAFIKYATLIMQRYKDKVTYWIPVNQINLTRVGLSSLGIVKDTVTQLEQK
                     KYQAIHNKFVVCAKIKEIGSKINNNFKFGCMLADFLVTPMTCKPKDVVFSTEKNQMTM
                     YFYADVQLRGEYPGYAISYFSHNHIAVEIKETDLVLIKENTLDFLAISYYNSNVVSHE
                     KNTLAIGDSQLNPYLETNPWGWTINPLGLYDCFLKYWDRYQKPLMIAENGIGQIERLD
                     NDTIHDDYRIDYIREHIIALNKAITRGVKVFAYCAWSPIDMVSSGTSEMKKRYGFIYN
                     DQDDYGVGSHKRYKKDSYIWYQNVIKSNGLKLE"
     gene            complement(2751..3092)
                     /locus_tag="PLK68_08720"
     CDS             complement(2751..3092)
                     /locus_tag="PLK68_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM90525.1"
                     /translation="MDENSLLIPVAMKIIMNSGNARTKANEALEALSIFDFSNAHKKI
                     VEAREDIKKAHQEQTEIIQKEAAGEHYKTCLLFTHAQDTLMTIMSEVNLTEKMIILFE
                     SFYQIQGHKEN"
BASE COUNT         1036 a          550 c          351 g         1177 t
ORIGIN      
        1 catcattaga aaccaccgct tcattagtat tttttatctc ttttaaatca gtatgatttg
       61 ttattaagac aggtgtttct aatgaatatc ccgcagcctt gatcttatcc atgtcaaatt
      121 ccaatagtaa ttatcctaga ccaatacgtg caataaagcc ctcgccattt aattgaactg
      181 cattaattcc cacgtaaatt aataattcaa caccaccttt ggggtaccgt aaagtgaaaa
      241 aagcgctcaa ttagtgacta atccagttaa tgctaatgtt gtaaaacata cctttcaaat
      301 tgatggagat ggctatggta ccttacaaat tgctcacact ttaacaagag aaacattcca
      361 acaccatctc tttctttaca tgaatttagt ttaaagaatg aaatagatga taaaagtaag
      421 tatactgatg tatggaataa tccctatatg tgtagaattt taatgaatgc aacttatctt
      481 agaaatttaa ttcttcataa aagggaatat ttcaaaacta gaggtgagta tcaaaaactt
      541 ccaaaagaga aatgaaaaat ctttaaacat catcatgaag taattataga cgaaaagata
      601 ttctgtattt atcaagcaaa attagaaagc aaggaagtac gttcaaaacg aaagatttat
      661 aaaaatatat ttcatggttt attatattgt gcagactgtg ataacctttt aaagacctta
      721 ttgtatcgct tttaccagta actgaaatca ttaaaaatag atcttcttta ttagcaatta
      781 aatcaattgc aaacaggtta tcgggctatt aacctctcca acacagcgaa ctcttcttgt
      841 ttattttact aaaacggcta actttctcat cattttatca ttaaggtata atgcatcaat
      901 tcgattgtat caatatattt tttcaatctt attattctct ttattaacgg tatctttagc
      961 attatttaag ctatttacat atttaacgca atcatattta aattctgcat aacctgaata
     1021 tcctactttt aacaaaatta ttgttttagt aactttcttc agttaatatt tctgttagcg
     1081 ataatcttta aatctttgtt aaacctttac aaatcaattc acacggtttt aattcatttt
     1141 tgttaataaa ttctttttta gattgatatt aaaaataaaa tccattgatc atcgcctcct
     1201 gtctctttag taattgcatc ttacaatgga atctttttca aattaattat caaaataaaa
     1261 ctatagtttc tttaaaaaac catagtttta tataaactat tctaatttta aaccattact
     1321 tttaataaca ttttgatacc atatatatga atctttttta tatcttttat gactgcctac
     1381 tccataatca tcttgatcat tataaataaa tccatatctt tttttcattt cacttgttcc
     1441 tgaagaaacc atatcaattg gcgaccaggc acaataagca aaaaccttga cacctcgtgt
     1501 gattgcttta ttcaaagcaa taatatgttc tctaatataa tcaattcgat aatcgtcgtg
     1561 tattgtatca ttatctaacc tttcaatttg accaattcca ttttcagcaa tcattaatgg
     1621 tttttgataa cgatcccaat atttaagaaa acaatcatat agaccaagag gattaattgt
     1681 ccatccccat ggattagtct ccagataagg atttaattgg gaatcgccaa tagctaaagt
     1741 attcttttca tgactgacaa cattagaatt ataataacta atcgctaaaa aatctaaagt
     1801 attttctttt attaatacta gatcagtttc ttttatctct acggcgatat gattatgact
     1861 aaaataactt attgcatatc caggatattc tccccttaat tgtacatctg cataaaaata
     1921 cattgtcatt tgattttttt cagttgaaaa aacaacatcc ttaggcttac aagtcattgg
     1981 tgttactaaa aaatcagcta gcatacaccc aaatttaaag ttattattta ttttacttcc
     2041 aatctctttt atttttgcac agacaacaaa tttattatga atagcttgat atttcttttg
     2101 ttccaattgt gtcactgtat cttttacaat tcctaacgaa ctgagaccta cccgtgttaa
     2161 attaatctga ttaacaggta tccaatatgt tactttatcc ttatatcttt gcataatcaa
     2221 agttgcatat tttatgaatg ctgtcacaac tttttcattt aaaaaaccat tatattttaa
     2281 agctaaatca attggcatat catcatgata taatgtaaca ataggcacta tccccatttc
     2341 ttttaacaaa ttaaatattt tatcataata ttttaatcct tgctcactag gttcaccatc
     2401 tattccattt ggaaatactc ttgcccaact tattgaaaac cgaaaacaag taatccccat
     2461 ttccttaagc aatatcaaat cctctttata acgatgataa aattcaattc ctattttttt
     2521 aggaaaatta tatctttctg gataattgag acggtctaaa atctccgctt tagataaatc
     2581 tcctttcacc tttgctttat catttggatc ataattttgc aaatcagcaa ctgtcaatgc
     2641 tctatcatca gccccctcag cctgttttgc gctcattgcc acgccccata aaaaatcttt
     2701 aggaaattga taatatattt gttttttgta tttttctgtt agtagcatat ttaattctcc
     2761 ttatgaccct gtatttggta aaaagattca aataatatta tcattttttc tgtcaaatta
     2821 acctcactca tgattgtcat aagagtatct tgagcatggg taaataataa acatgtttta
     2881 taatgttcac ctgcagcctc tttttgaatt atttctgttt gttcttgatg tgcttttttt
     2941 atatcctccc ttgcctccac aattttctta tgtgcatttg aaaaatcaaa gatactgagt
     3001 gcctccaatg cttcatttgc ttttgttctt gcattgcctg aattcataat gattttcatc
     3061 gcaactggta ttaaaagact attttcatcc atttattatt cctccacact atat
//
LOCUS       DAOMTA010000306         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1275_length_3107_cov_2.716252, whole genome shotgun sequence.
ACCESSION   DAOMTA010000306 DAOMTA010000000
VERSION     DAOMTA010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_3107_cov_2.716252"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..699)
                     /locus_tag="PLK68_08725"
     CDS             complement(127..699)
                     /locus_tag="PLK68_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRM90526.1"
                     /translation="MHLVPTVIEKTNQREYAYDIYSRLLEDRIILLTGTIDDKMSSSI
                     VGQLLYLESLDNNADIFMYINSPGGSINAGMAIYDTMNFIKCDVSTIVIGMAASMAAF
                     LLSAGAKGKRCSLPNSEIMIHQPLGAFEGQASDIEISAKRILKQKEKLNLILSKNTNQ
                     PIDKIVIDTDRDHFLEPDEALEYGLIDEVI"
     gene            976..1992
                     /gene="gap"
                     /locus_tag="PLK68_08730"
     CDS             976..1992
                     /gene="gap"
                     /locus_tag="PLK68_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRM90527.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTDPKMLAHL
                     LKYDSAQGRYALADKVEAGENSITVDGKEIKIYSEADASKLPWGEIGVDVVLECTGFY
                     VSKAKSQAHIDAGAKKVVISAPAGNDLPTVVFGVNENILTADDTIISAASCTTNCLAP
                     MANALNNLAKIKSGIMLTVHAYTGDQMVLDGPHRKGDLRRARAAAVNIVPNSTGAAKA
                     IGLVIPELNGKLIGSAQRVPVPTGSTTILTSVVEGEVTVEEVNAAMKAAATPSFGYTE
                     EQLVSSDIIGINYGSLFDATQTMVKPMDNGTTEVQTVAWYDNENSYTSNMVRTIKYFA
                     ELSK"
     gene            2056..2964
                     /locus_tag="PLK68_08735"
     CDS             2056..2964
                     /locus_tag="PLK68_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRM90528.1"
                     /translation="MIKRVGIIGMGALGMLYGDFIAKNITREAVTFIVDEKRKKAYQE
                     TVFTINGEKVLFNLVEYFNAVPFDLIIIVVKGTALEEAIKEIKKCVDKNTIIISVLNG
                     IDSEKVISEYYGREKVIYAVAQGMDAMKFGTSLKYTKEGQLLLGITDFKQKDKLDKFT
                     SFFDQAKINYEVKGDILHSMWAKFMLNVGINQTCMAYNTNYSDALMPGEANDTLIAAM
                     KEIIELSHYEGVDLTETDLKHYIEIIRTLDPLGLPSMAQDAKVRRYSKVEMFAGTVIS
                     LASKHKLKVPTNEFLYKRIRGIENSY"
BASE COUNT         1094 a          437 c          585 g          991 t
ORIGIN      
        1 aaatggatgc cacctgtttt gtacagatta gcatccatgg cataattttt ttaaaactaa
       61 atttaaacgt gtccagaaaa ctgggtacac atcatttttt ggggttcact tcataagttg
      121 gtcgttttaa ataacttcat caattaagcc gtattcaagc gcttcatcag gttctaaaaa
      181 gtgatcacga tcagtatcga taacaatttt atctatgggc tgatttgtat ttttactaag
      241 aattaaattt aatttttcct tttgttttaa gattcgtttg gcactaattt caatatcact
      301 cgcttgtcct tcaaaagctc ctaatggctg atgaatcata atttcactat taggtaaact
      361 acaacgctta cctttagctc cggcactgag taaaaaggca gccatactag cagccatccc
      421 aattacgatt gttgaaacgt cacatttgat aaagttcatt gtatcataga ttgccattcc
      481 cgcattgata ctgccacctg gggaattaat atacataaaa atatcagcat tgttatctaa
      541 tgattctaaa tagagaagct gaccaacaat actgctggac atcttatcat caatagtacc
      601 ggttaataag atgatccggt cttctaatag tcgtgaatat atatcataag catattcacg
      661 ttggtttgtt ttttcaatta ctgtaggaac taaatgcatt atcttacctc cacatttaag
      721 tatagtaatt aaaataataa ttattcattt aagcctataa aaggcaagaa agattgcggt
      781 aaaactaggc ttgtaaaagt cactaaattt atattataaa agaataatgt tagattttgt
      841 tccattaaag ttaaatttat gaatcttttg tgaaatattt aatttttttc aatgaaaccc
      901 gttacattag ttgcttttct tgcctttctg gaatataata tgagcataaa gaacattcaa
      961 ggaggaatat aaagaatggc agtaaaagta gcaattaacg gatttggacg tatcggacgt
     1021 cttgcattca gacaaatgtt tggtgctgaa ggatatgaag ttgtagcaat caacgattta
     1081 actgatccta aaatgttagc acatttatta aaatatgatt cagcacaagg tagatatgca
     1141 ttagctgata aagttgaagc tggtgaaaat tcaatcactg ttgatggaaa agaaattaaa
     1201 atctatagtg aagcagatgc ttctaaatta ccttggggtg aaatcggtgt agatgtagta
     1261 ttagaatgta ctggtttcta tgtatctaaa gctaaatctc aagctcatat tgatgcagga
     1321 gctaaaaaag ttgttatctc tgcaccagca ggaaatgact taccaacagt tgtattcgga
     1381 gtaaacgaaa atatcttaac tgctgatgat acaatcatct ctgcagcatc ttgtacaact
     1441 aactgtttag caccaatggc taacgcttta aataacttag ctaaaattaa atctggtatc
     1501 atgttaacag tacatgctta cacaggtgac caaatggtat tagacggacc tcatagaaaa
     1561 ggtgacttaa gaagagctcg tgcagctgca gtaaatatcg tacctaactc aactggtgca
     1621 gcaaaagcta tcggattagt tatccctgaa ttaaacggaa aattaattgg ttctgctcaa
     1681 cgtgtacctg ttcctactgg atcaactaca atcttaactt cagttgttga aggtgaagtt
     1741 acagttgaag aagtaaatgc agctatgaaa gcagcagcta ctccatcatt tggatatact
     1801 gaagaacaat tagtttcttc tgatattatt ggaattaact atggttcatt atttgatgca
     1861 actcaaacta tggttaaacc aatggacaac ggaactactg aagttcaaac agttgcttgg
     1921 tatgataatg aaaattcata tacttctaac atggttagaa caatcaaata tttcgctgaa
     1981 ttatctaaat aattaattga aacttgatgc aaaagctggt cattgatcag cttttttctt
     2041 atggagaata ttattgtgat caaacgagtt ggaattattg ggatgggtgc tttaggaatg
     2101 ttatatggag attttattgc aaaaaacatt actagagaag cagtgacatt tatagttgat
     2161 gaaaaaagga aaaaagcata tcaggaaact gtatttacaa taaatggtga gaaagtttta
     2221 tttaatttag ttgagtattt taatgccgtt ccatttgatt taataatcat agtagtaaaa
     2281 gggactgctt tagaagaagc aataaaggaa attaaaaaat gtgttgataa aaatacaata
     2341 ataatttctg ttttaaatgg gattgacagt gaaaaggtta ttagtgaata ctatgggaga
     2401 gaaaaagtaa tttatgcggt tgctcaggga atggatgcaa tgaagtttgg aacatctcta
     2461 aagtacacta aagaaggaca gctcttattg ggaatcactg atttcaagca aaaagataag
     2521 ttggataagt ttactagctt cttcgatcaa gcaaaaatta attatgaagt taaaggcgat
     2581 attttacata gtatgtgggc taaattcatg ttgaatgttg ggataaatca gacttgtatg
     2641 gcatataata ccaattatag tgatgcttta atgccggggg aagcaaatga taccttgatt
     2701 gcagcaatga aggaaattat tgagttgtca cattacgagg gagttgatct aactgaaacg
     2761 gatttgaagc attatattga gataattagg actttggatc ctctgggttt accatcaatg
     2821 gctcaagatg caaaagtacg tcgttacagt aaagttgaaa tgtttgctgg aacagtaatt
     2881 tctttggcat caaagcataa actaaaagta ccaacaaatg aatttttata taagcgaata
     2941 aggggcattg aaaatagtta ttaaaatgaa tatttaatgc tgctaagaaa ttataaagaa
     3001 agaggagtat taattatggc taaatttgga tatgttaaaa tcgtctcagg acaagcagca
     3061 agacgaattg gaaggtttat tggctgggat gaaaaagggg ttaaagc
//
LOCUS       DAOMTA010000307         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1277_length_3105_cov_1.925902, whole genome shotgun sequence.
ACCESSION   DAOMTA010000307 DAOMTA010000000
VERSION     DAOMTA010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_3105_cov_1.925902"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLK68_08740"
     CDS             <1..262
                     /locus_tag="PLK68_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM90529.1"
                     /translation="ADLVLQVLSYVAQIERENTRQRQAEGIREAKRRGVRFGRPKLTI
                     PDDFEEIYYLWRKNVISKAEASRRLSTNNHTFTRWVKDYESI"
     gene            557..>866
                     /locus_tag="PLK68_08745"
     CDS             557..>866
                     /locus_tag="PLK68_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90530.1"
                     /translation="MERIIIRKDMILTTILILSIIPFSLIMISIPIVASGFISNVSSA
                     IMIFCLFIFGVLEFFSLVIVIVGTIELLELIFFGMKKFVIDKDGITSYRLWKKVILWQ
                     K"
     assembly_gap    867..884
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1124..2752)
                     /locus_tag="PLK68_08750"
     CDS             complement(1124..2752)
                     /locus_tag="PLK68_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRM90531.1"
                     /translation="MKRKKLKLKKQAYFVIGGLVILAALILYLILCLVTGGDNFVSNT
                     TINGIKVGNMNKEEARKAIEQQYQNDLNPPTLTLLLDQQEYPVDLTDNLTFDSKKAVD
                     KISKQSNSSFFRRGYNYLFNHDYTVGVKIKNKDLLNEKITNSQILNYSTLTPTNYELK
                     DDKIIFTKGKSGKTAELESVFNTINKALNNYDFKGKIKYSPVEHKLNDEEIKLIHENL
                     SKEAKNATLDKNNNYEIIDSQVGAKFDLEDAIAKYNKTTEGKQFTLNATIIKPEITKE
                     MLEQNLFKDVLGEYATNVSGTSVRKNNVKLSGDKCNGVILLPGEEFSYNNVVGKRTKE
                     NGFGEAAAYLNGETVQEVGGGICQTSSTLYNAVLYANLKITERTNHTFVSGYVPIGRD
                     ATVSWGGPDFKFKNDQAYPIKIIASYENSRLTTKILGTNVNNIRVELKSQKLASTNYN
                     TKYEDDPTLPEGQTKVKQKGTPGSKAKSWRYVYDANGNLISSNEEAYSVYKGHEEIIL
                     RGTMKITEPATPAPTEPTTPAQPETPTIPNNETS"
     gene            2911..>3105
                     /locus_tag="PLK68_08755"
     CDS             2911..>3105
                     /locus_tag="PLK68_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     aminotransferase"
                     /protein_id="HRM90532.1"
                     /translation="MYNCIQFFTKSLYKLDCIQYNRLHIQERKGIYMTEKRVLNFSAG
                     PSMLPEPVLEKAAKQMLNYEN"
BASE COUNT         1030 a          427 c          468 g         1162 t
ORIGIN      
        1 tgcagactta gtattgcaag tgctttctta tgttgctcaa atagaacgcg aaaatacacg
       61 tcaaagacaa gccgagggaa ttcgtgaagc taaacgtaga ggcgtacgat ttggaagacc
      121 aaaattaacg attcccgacg attttgaaga aatttattat ttatggcgaa aaaatgtaat
      181 cagcaaagct gaagcttcgc gtcgtttaag tacaaataat cataccttta ctagatgggt
      241 aaaggattat gaatcaatat aaataaagtt atttaagtaa aaacaggaaa aaatgtagac
      301 cttttttcct gtttttttca atcataatat ttgtgaaaat taagaaaata tcttatttaa
      361 gtttgtttat ttaattatat attctatgtg agaaaaagtc tactttgctt cctaaaaaga
      421 aatactttat attacaaaac actcgaatac aaaccctgat atttgtattc gtaaacaata
      481 tttaattatt ttcaagttta cagaaaataa agtatgtttt aatgtaatga cttgaatggt
      541 ggaaaggata aaagaagtgg aaagaattat tattagaaaa gatatgattt taacaactat
      601 tcttatttta tccataattc cattttcatt aattatgata agcattccaa tagtagcttc
      661 aggatttatt tcgaatgtat catcagctat catgatattt tgtttattta tatttggggt
      721 gctagagttc ttttctttgg taattgtaat agttggaact attgaattat tagaattgat
      781 attttttgga atgaaaaagt ttgtaattga taaagatggt atcacttctt ataggctatg
      841 gaaaaaagtg attttatggc agaaggnnnn nnnnnnnnnn nnnntcagct gcaccatgag
      901 cctttttatt ttctgataat tcttgaaaag aatggtaaga atatggaaat aaatctagct
      961 agtgcttcgt taatgatttc agaacaagag atatatcagt attgtttaaa aaagtggaag
     1021 attgcacaaa aataaaataa ttaaaaagat tattatttta tgtaataatc tttttaatta
     1081 tagaaaaagg tggaaaaatt ccaccagtat ttaatttaag attctacgat gtttcattat
     1141 tgggaatggt aggcgtttct ggttgtgctg gagttgtagg ttctgttggt gcaggcgtag
     1201 ctggttcagt tattttcata gtacctctta aaataatttc ctcatgtcct ttatatacac
     1261 tatatgcttc ttcatttgat gaaattaaat tgccattagc atcataaaca taacgccatg
     1321 attttgcttt actacctgga gttccttttt gttttacttt tgtctgtcct tcgggtaatg
     1381 taggatcgtc ttcatattta gtattataat tagttgaagc aagtttttga ctttttagct
     1441 caacacgaat attattaaca ttagtaccta aaattttagt tgttagacga ctattttcat
     1501 aagaagcgat gatcttaata ggataagcct ggtcattttt aaatttaaaa tcagggccac
     1561 cccaagatac tgtagcatca cgaccgattg gaacatagcc agaaacaaaa gtatgattag
     1621 tccgctcagt aatctttaga ttagcgtaca atacagcatt atacaaagtt gatgatgtct
     1681 gacagattcc acctccaact tcttgcacag tctctccatt taaataggct gcagcttcac
     1741 cgaagccatt ttctttcgtt cttttgccaa caacattatt ataagagaat tcctcacctg
     1801 gcaataaaat aactccattg catttatcac cagaaagttt aacattattt ttacgaactg
     1861 aagtaccgct gacgttagtc gcatattcac ctaaaacatc tttaaataaa ttttgttcta
     1921 acatttcttt ggttatttca ggtttaatga tcgtagcatt tagggtaaac tgtttacctt
     1981 cagtagtttt attgtattta gcaatagcat cttctaaatc aaatttagca cctacttggc
     2041 tatcgatgat ttcataattg ttatttttat ctagagtagc atttttagct tcctttgata
     2101 aattttcatg aattagtttt atttcttcat cattaagttt atgttctact ggagaatatt
     2161 taattttccc tttaaaatca taattattta atgccttatt aattgtattg aaaactgatt
     2221 ctaattctgc tgttttaccg gattttcctt tagtgaaaat aattttatca tctttaagtt
     2281 catagttagt aggagtcaaa gtactataat ttagaatttg actattagta attttttcat
     2341 ttaaaagatc tttatttttt atttttaccc caactgtata atcatgatta aacaaatagt
     2401 tatatcctcg tctgaagaat gaactattag actgtttaga aattttatct acagcttttt
     2461 tactatcgaa tgtaagatta tcagttaaat caactgggta ttcttgttga tcgagtaata
     2521 aagttaaagt agggggattt aaatcatttt gatattgttg ttctatagcc ttacgtgctt
     2581 cttctttatt catattacca actttaatac cattgatagt tgtatttgaa acaaaattgt
     2641 ctccccctgt tactaaacat aaaataaggt atagtataag ggcagctagg atgacgaggc
     2701 caccaataac aaagtaagct tgttttttca attttagttt ttttcttttc attttttctc
     2761 tcctatatgt accttttcat catatcttag aaatgtattt aattcaattt attttatcgt
     2821 aaatgtagtt aaaatatcgt gaatttatca aatgttccag catataatga aactgataat
     2881 tactaagaaa aatagctttt ttatgaaacg atgtataatt gtatacaatt ttttacaaaa
     2941 tccctttaca aattagattg tatacaatat aatagacttc acatacaaga aaggaaaggt
     3001 atatatatga ctgaaaagag agtactgaat ttttctgcag gtccttcaat gttgcctgag
     3061 ccagttttag aaaaagctgc taaacaaatg ttgaactatg aaaat
//
LOCUS       DAOMTA010000308         3104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1278_length_3104_cov_2.449984, whole genome shotgun sequence.
ACCESSION   DAOMTA010000308 DAOMTA010000000
VERSION     DAOMTA010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_3104_cov_2.449984"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLK68_08760"
     CDS             <1..580
                     /locus_tag="PLK68_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM90533.1"
                     /translation="IEFKGLEFSKMKEIFEAGLNFDVCIHVYTPDNLYVFNLSESERQ
                     RIKNQKLEAVYPEVNSVDFLKDTPISKILYQNVDVPYLMSLEEGLKPITDGHCAVSYS
                     SNRYMEFNALGVDKGQGLIDLANKLGIAIEETIAVGDNYNDMAMLKVAGLSVAANNAV
                     DDVKAACDYTTNANNNEGVVAELIEKFIFNEI"
     gene            570..1229
                     /locus_tag="PLK68_08765"
     CDS             570..1229
                     /locus_tag="PLK68_08765"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRM90534.1"
                     /translation="MRFKDIVEIEFQQDYYKQLHQFVENEYLHKTIFPPKENIFRALN
                     LCDYENVKVVILGQDPYHELHQANGLAFSVYPGVRIPPSLVNIYKELQDDLGTMIPNH
                     GDLTKWAKQGVLLLNNVLTVEEGRANSHSGKGWETFTLNIVKALNKRQKPLVFILWGN
                     NARAKKQYIDTSRHLVLESAHPSPLSAHRGFFGSHPFSKANEFLIRNGMPMIDWQIDN
                     I"
     gene            1359..1493
                     /locus_tag="PLK68_08770"
     CDS             1359..1493
                     /locus_tag="PLK68_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90535.1"
                     /translation="MNSGIGIIWAPVSAGTLVIWGLGIFVAVVGMVWFVKRLKKKIQL
                     "
     gene            1601..2764
                     /locus_tag="PLK68_08775"
     CDS             1601..2764
                     /locus_tag="PLK68_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRM90536.1"
                     /translation="MFNDIKSALVYIESKRTKRTLEQFKETIKKYGFNIYQKNIIHIA
                     GTNGKGSTTNFIKDIMVEHGYRVGTFTSPYLICHNDRICINGEMISDERLLMIINSLV
                     EIIESEQLSMFEIDILIMLRYFDEVDYRIIETGIGGLNDKTNVIDSVCSVITNIGYDH
                     QFMLGDTLAQIAHHKAGIIKPEQTCFTSEINNDLIDIFKAAAEIKNSQVVSVEIDCDY
                     RYPYHFSCLGYDYQLSNCGSYQVANATLAINVCDYLIELDHSLVQRALDGFSWPGRFE
                     KFGKIYLDGAHNIDGIKALIKTLHDQQIKKALVIFSALGDKDIEQMEALLSEYPLIQA
                     TFADERLQLEGIDFKEAIKNNIDLYDHLIITGSLHFISTVRKYLQNNLIEKQV"
     gene            complement(2791..>3104)
                     /locus_tag="PLK68_08780"
     CDS             complement(2791..>3104)
                     /locus_tag="PLK68_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90537.1"
                     /translation="SIYINQIFFIINAFLGIFGLVSILISEALTPPNNAYTCCGNLAT
                     LGSSGLILISLLAIIIEAYAYTSVISLILAGSFFFLVLMLGGIWCYLNLRNHCHRNYY
                     C"
BASE COUNT         1081 a          369 c          627 g         1027 t
ORIGIN      
        1 tattgagttc aaaggtttag agtttagtaa gatgaaagaa atttttgaag caggattaaa
       61 ttttgatgtt tgtatccatg tttatacgcc tgataattta tatgtgttta atttaagtga
      121 atcggaacgc caaagaatta aaaatcaaaa gttagaagcg gtatatcctg aagttaatag
      181 tgttgatttt ttaaaggata caccaatttc taagattttg tatcaaaatg ttgatgttcc
      241 ttatttaatg agtttagaag aggggttaaa accaattact gatggacatt gtgcggttag
      301 ttattcatca aatcgttaca tggaattcaa tgctcttggg gtggataaag gacaaggatt
      361 gattgattta gcaaataaat taggcatcgc tattgaagaa acgattgctg ttggtgataa
      421 ttataatgat atggcaatgt taaaagtggc gggcttatca gtcgctgcta acaatgctgt
      481 agatgatgtt aaagctgcat gtgattatac aacaaatgct aacaacaacg aaggtgttgt
      541 ggcagaattg attgagaaat ttatttttaa tgagatttaa agatattgtt gaaattgagt
      601 ttcaacaaga ttattataag cagctacatc agtttgttga aaatgaatat ttacataaaa
      661 cgatttttcc cccaaaagaa aatattttta gagctttaaa tctttgtgat tatgaaaatg
      721 ttaaagtagt tattttagga caagaccctt atcatgaact tcatcaggca aatggattgg
      781 cattcagtgt ttatccgggg gttcggattc ccccaagttt ggttaatatt tataaagagt
      841 tacaagatga tttaggaaca atgattccta atcatggaga tctcacaaaa tgggctaagc
      901 aaggagtctt gttgcttaat aatgtgttaa cagttgaaga agggcgggct aatagtcatt
      961 ctggtaaagg ttgggaaact tttactttaa atattgttaa agctttaaat aaacgccaaa
     1021 agccgctagt atttatttta tggggaaata atgcccgtgc taaaaaacaa tatatcgaca
     1081 cttcacgtca tttggtttta gagagtgcgc acccttctcc actatcagca catcggggat
     1141 tctttggttc acatcctttt agtaaggcaa atgaattttt gattaggaat ggaatgccaa
     1201 tgattgattg gcaaattgat aatatttaaa ggttggttta tcagtaatta tactgataaa
     1261 ggcaaccttt ttcttaagtt ttcttaatcg ttataggatt cattaaaaaa atatattaaa
     1321 tatgttataa ttaaaagtga aaaaaggagg ggactaaaat gaatagtggt attgggatta
     1381 tttgggcacc ggttagtgct ggaacattgg ttatttgggg acttggaata tttgttgcag
     1441 ttgtaggaat ggtgtggttt gttaaacggc tcaaaaaaaa gattcaatta taagataaaa
     1501 gagggatttc gtaaaagaag tccttttatt ttgaatcctt atttgtcatt atagataaat
     1561 tgtattagaa taagttaaat tacttgcaga ggagaaaata atgtttaatg atattaaaag
     1621 tgcgctagtg tatatagagt caaagcgaac taagcggacc ttggaacagt ttaaagagac
     1681 aattaaaaaa tatggcttta acatttacca aaaaaatatt attcatatag ctggaactaa
     1741 tggtaaaggt tcaacaacta attttattaa agatattatg gttgaacatg ggtatcgtgt
     1801 gggaacattt acatcaccat atcttatttg tcataatgat cgtatttgta ttaatgggga
     1861 aatgataagt gatgaacgac tgctgatgat tattaacagt cttgttgaaa ttattgaaag
     1921 cgaacaatta tcgatgtttg aaattgatat tttgatcatg ttgcgttatt ttgatgaagt
     1981 agattatcga attattgaaa caggaatcgg gggattaaat gataaaacta atgtaattga
     2041 tagtgtttgc agtgtgataa ctaatatcgg ttatgatcat cagtttatgt taggtgatac
     2101 tttagcacaa atagctcatc ataaagctgg aatcattaaa cctgaacaaa cttgttttac
     2161 tagcgaaata aataatgatt tgattgatat ttttaaagct gcagctgaaa ttaaaaatag
     2221 tcaagtagtt agtgtggaga tagattgtga ttatcgatat ccttatcatt ttagctgttt
     2281 aggttatgat taccagttaa gtaattgtgg tagttatcag gttgctaatg cgacattggc
     2341 gattaatgta tgtgactatt tgattgagtt agatcattcc ttagtacaac gagctttaga
     2401 tggatttagc tggccaggcc gatttgaaaa gtttggaaag atttatttag atggggcaca
     2461 taatatcgat ggaatcaagg cactaattaa gacattacat gatcaacaaa taaaaaaggc
     2521 attagttatc ttcagtgctt tgggggataa agatattgaa caaatggaag ctttattaag
     2581 tgaatatcca ttgattcaag caacatttgc agatgaacgg ctacaattag agggaattga
     2641 ttttaaagaa gcaattaaaa ataatattga tctatatgat catttaatta ttacaggctc
     2701 gttacatttt attagcacgg tccgtaaata tcttcaaaat aatttaattg aaaagcaggt
     2761 atgattcaaa tttacctgcc tttttgtttg tcaacaataa tagttacgat gacagtgatt
     2821 gcgaaggttg agatagcacc atataccgcc tagcatcaaa actaagaaga aaaaactccc
     2881 agcgagaatt aggctaataa cacttgtata agcataggct tcaatgataa ttgctaatag
     2941 agaaattagg attaagccac tactgccgag cgtagctaga ttaccgcaac aagtataagc
     3001 gttatttggc ggggttaaag cttcgctgat taagatactg acgagtccaa agataccgag
     3061 gaatgcattg atgataaaga aaatctgatt gatataaatt gaac
//
LOCUS       DAOMTA010000309         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1281_length_3098_cov_2.310220, whole genome shotgun sequence.
ACCESSION   DAOMTA010000309 DAOMTA010000000
VERSION     DAOMTA010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_3098_cov_2.310220"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLK68_08785"
     CDS             <1..201
                     /locus_tag="PLK68_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90538.1"
                     /translation="DGVELLGYTPWGCIDLVSASTGEMSKRYGFIYVDRDDQGNGSLK
                     RSKKKSFAWYKKVIASNGTDLD"
     gene            complement(234..680)
                     /locus_tag="PLK68_08790"
     CDS             complement(234..680)
                     /locus_tag="PLK68_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90539.1"
                     /translation="MYKINDYIIHKNNHLYQIIAIHNHTCILTTWQTNETIITNITEL
                     VRKVITASEMEEIVERIPYIRTLNITSERYRQELYQKSLDKYDEVEWIKLIKTIYIRH
                     QKKQTQNYELKYFKAAKNIFHEEISLLLNIPLPKIETYIKQKILEN"
     gene            complement(690..1190)
                     /locus_tag="PLK68_08795"
     CDS             complement(690..1190)
                     /locus_tag="PLK68_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRM90540.1"
                     /translation="MYKIGDTVLYGREGVCKIKDIVTRKLNNIDKQYYFLTPLDDHIT
                     ILVPTDNEEALSKMRKVLSKKDIYELIKTMPDNETIWINDKNIRKQKYNDIINHGNHE
                     QLVKLTKTLYLNKQKQEKAGKKFHVQDQHFLQTAEKMLYDEFCHTLNLKPEEILPFIL
                     ETLKEN"
     gene            1448..2998
                     /locus_tag="PLK68_08800"
     CDS             1448..2998
                     /locus_tag="PLK68_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRM90541.1"
                     /translation="MKRLKRLLAMVVSFALVATTYCTPLFAVDAPDTYSCQLYGEVTD
                     AGEVVSKMVIDYGAAKKVTGVGLDTFTVHAKASTEDIRQGTQDTSYGDYDLDRKIVKV
                     EVEGQYVNIYFNQSEGATLAYLSSGRNYPAELTYTVTQNKPVTLTAADGTVINDSYTG
                     NYTCDNTVINAETAKFESVKVKDGINYQYYDAGSADSLIVWFHGNGEGDYLSSGNNVA
                     QMLANRGTVAWATDETQNIFGGAHVMAFQAPDTWYYAQNDGLLEKAYNEINEVIKAKN
                     IDPDKVFVSGCSAGGYMTTRMLIAYPDLFAAAMINCPALDVADARGGETPTDAELASI
                     KNSDTAIWLVQGKTDSSVKPEDCSIRLFNALTDGQELITSEHKQDLNSDFTTSETKDG
                     KYKLSLYETVAVTDPSTGASSNKLEFAEDYDQDGVATLVQYSDHWSWIYTLNNNPKDA
                     KGVSIMNWAAEYGKTAEQPTPAPVPETPADTPKTSVKTGDSVSLGLYAITTLAGLCGI
                     ALLKKRYN"
BASE COUNT         1051 a          405 c          556 g         1086 t
ORIGIN      
        1 gatggagttg aattattagg ctatacacct tggggatgta ttgatttagt gtctgcttca
       61 actggtgaaa tgtcaaaacg ctatggattt atctatgttg atcgagatga tcaagggaac
      121 ggttcattaa aacgttcaaa gaaaaaaagt tttgcctggt ataaaaaagt tattgcttca
      181 aatggaactg atttagatta attaaaagca ttgactcata aaagtcaatg tttttaattt
      241 tctaaaattt tctgttttat gtatgtttca atcttaggta gtggaatatt tagaagaagt
      301 gatatttctt catgaaaaat atttttggct gctttaaaat attttagttc ataattttga
      361 gtctgttttt tttggtgacg aatatagatt gttttaatta gcttgatcca ctcaacttca
      421 tcatatttat ctagtgactt ttggtaaagc tcttgtcgat atcgttcact agttatgttt
      481 aaagtacgaa tatatggaat tcgttcaaca atttcctcca tttcagaagc tgttataacc
      541 tttcggacaa gttcggtgat attcgtaatg attgtttcat tggtttgcca agttgttaaa
      601 atgcaagtgt gattgtgaat agcgataatt tgataaagat gattgttttt atgaataata
      661 tagtcattaa ttttatacat aaatgctcct taattttctt tcaaagtttc taaaataaat
      721 ggtaaaattt cttctggctt taaattgagt gtatgacaaa attcatcata aagcattttt
      781 tctgcggttt gtaaaaaatg ctgatcttga acatgaaatt tttttccggc tttttcttgt
      841 ttttgtttat ttaaatacaa agttttggtc aatttaacta attgttcatg attcccatga
      901 ttaataatat cattatattt ttgttttcga atatttttat cattgatcca gattgtttca
      961 ttatctggca tcgtcttaat taattcataa atatctttct tagataatac ttttcgcatt
     1021 tttgataaag cctcttcatt atctgttggg acaagaatag ttatatgatc atccaatggg
     1081 gttaaaaaat aatattgctt atcgatattg ttaagtttgc gagtaacaat atccttgatc
     1141 ttacaaacac cttcacgtcc atataaaacg gtatcgccaa ttttgtacat ataaattcct
     1201 ccatataata aaaaagtgta gctgcaactg gacttacgtt ccgcaaacta cacgttgaga
     1261 taatataatt atatcattta aaaaaataat ttcatgtatc aattttaaaa tcattatcat
     1321 aaatagattt taaattcaat agttttttta atttttaggt acctatttat ggtacttagt
     1381 aagtattttt gtaatcgctt tcaatgagaa agggtgataa actaaatata tcgaggagga
     1441 aagagatatg aagagattaa agagattatt agcgatggtc gtaagtttcg cattagtagc
     1501 aacaacatat tgcaccccat tatttgctgt tgatgctcca gatacttata gctgtcaatt
     1561 atatggggaa gtgacagatg cgggtgaggt cgtatcaaaa atggtaattg actatggtgc
     1621 tgctaaaaag gttacaggtg tagggttaga tacatttact gttcatgcta aagcatcaac
     1681 agaagatatt cgtcaaggta cacaagatac tagctatggt gattatgatc ttgaccgtaa
     1741 aattgttaaa gtagaagttg aaggacaata tgtaaacatc tattttaatc aaagtgaagg
     1801 ggccacttta gcttatttaa gttcaggtag aaattatccg gctgaattaa cttatacggt
     1861 tacacaaaat aagccagtta ctttaacagc tgcagatggt acagttatta acgattcata
     1921 taccggtaat tatacttgtg ataatactgt aataaatgca gaaactgcta aatttgaatc
     1981 agttaaagta aaggatggaa ttaattatca atattatgat gcaggtagtg cagattcatt
     2041 gattgtttgg ttccatggta atggtgaagg tgattattta tcttctggca acaatgttgc
     2101 acaaatgtta gctaatcgtg gtacagttgc ttgggctaca gatgagactc aaaatatttt
     2161 tggaggggct catgttatgg ctttccaagc accagatact tggtattatg cacaaaatga
     2221 tggtttatta gaaaaagcgt ataatgaaat taatgaagta atcaaagcta aaaatatcga
     2281 tcctgataaa gtgtttgttt caggatgttc agctggtggt tacatgacaa caagaatgtt
     2341 gattgcttat ccagatttat ttgctgctgc aatgatcaac tgtcctgcat tagatgtagc
     2401 agatgctcgt ggtggagaaa ctccaactga tgcagaatta gcaagtatta aaaattctga
     2461 tactgcaatt tggttagttc aagggaaaac agatagcagt gttaaaccag aagattgctc
     2521 aatccgttta tttaatgctt taacagatgg acaagaatta attacttcag aacataaaca
     2581 agatttaaat tctgatttta caacttctga aactaaagat ggaaaatata aattatcttt
     2641 atatgaaaca gtagcagtaa cagatcctag tacaggagca agttctaata aattagaatt
     2701 tgcagaagat tatgatcaag atggtgtagc aacattagtt caatatagtg atcactggtc
     2761 ttggatctat acattaaata acaatcctaa agatgctaaa ggtgttagta ttatgaattg
     2821 ggctgctgaa tatggaaaaa cagcagaaca accaacacct gcaccagtac cagaaacacc
     2881 agctgataca cctaaaactt cagttaagac tggtgatagt gtgagccttg gattatacgc
     2941 aataactaca ttagcaggac tttgtggaat tgcattatta aaaaagagat ataattaaaa
     3001 gtagtggact tagttgagaa actaagttct tttttgatta aattgttaaa ttagactaac
     3061 ttattattga aaagatgaaa agtaaatgtt aaagtatg
//
LOCUS       DAOMTA010000310         3081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1293_length_3081_cov_1.908790, whole genome shotgun sequence.
ACCESSION   DAOMTA010000310 DAOMTA010000000
VERSION     DAOMTA010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1293_length_3081_cov_1.908790"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLK68_08805"
     CDS             <1..998
                     /locus_tag="PLK68_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM90542.1"
                     /translation="EVARMYGYDKIPSTLPEMSMTVGKRTDVQAKKHLIRNLLKDQGL
                     HETLTYSLTSPVMVDDFNIFHTNESVKLAMPLGEERSVTRKSIIGSLLQVINYNQSHN
                     IKDVNIFELSTTYSKGVELQNLAIACCGEYNGLPFKQISYKADYYLVKGFVETIFKNL
                     GIEESRYKLVRASQDDKYYHPGRTAYIMIGKEVVGVVGNIHPLMEKKYNVKDVYIVEL
                     NLTTLLNLKTSKVKFTEIPMYPSVSRDIALVMDQDIPTFDICRKIVQASKQLVKETKI
                     FDVYAGEHIEAGKKSVAINLLFQDPKGTLEEATVNAAMEKILAAVEKDFGAVLRA"
     gene            1118..1375
                     /locus_tag="PLK68_08810"
     CDS             1118..1375
                     /locus_tag="PLK68_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRM90543.1"
                     /translation="MQKITLEIDGMMCGMCESHINDAIRKAFSVKKVSSSHSKDRTEI
                     ITADELDEDKLKAVIDATGYKVTSITKAPYVKKGFFASFRK"
     gene            complement(1384..3003)
                     /locus_tag="PLK68_08815"
     CDS             complement(1384..3003)
                     /locus_tag="PLK68_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HRM90544.1"
                     /translation="MEYPRPQLKRNSFFSLNGVWQLNDLSINIPYPPQAPLSNYHGII
                     NDTLVYQKDFILPIDFYHANNQVLLHFGAVDQLTKVYVNNRYIGSHSGGYLPFYFDIS
                     SSLNDGVNKLVVKVTDSLSHDYPYGKQSRESHGMWYTQVSGIWQTVWVEAVSKNCIKD
                     LLITPSLTGINLKVNTEASRYTVTIENNDFKKTYTAKDTDITIDNPHYWTPDDPYLYT
                     FSITTDDDYIESYFALRTVEIKKHQILLNDTPIFLHGILDQGYYHDGLFLPEHPDFYK
                     TEIMNIKKLGFNLIRKHIKIEPELFYYNCDKLGILVMQDMVNNGEYRFIHDTVLPTLG
                     FINFKDTRYKNTLQQNIFKQHMIDTISHLYNHPSIISYTIFNEGWGQFNSDEMYQICK
                     ELDPTRFYDTTSGWFSQKNSDVVSRHIYFRNKILSTNLERPLLLSECGGYTRPINNHL
                     NSNKKQYGYGKTNSEKELTDKIEKMYQEMVIPSINNGLCGCIYTQISDIENEINGLYT
                     YDRKICKVNQQRMSELARKLYSYYEKKSGLS"
BASE COUNT         1060 a          419 c          546 g         1056 t
ORIGIN      
        1 aagaagtagc acggatgtat gggtacgata agattccttc aacattacca gagatgtcga
       61 tgactgttgg aaagaggaca gatgttcaag caaagaaaca tttaattaga aacttattaa
      121 aagatcaagg gttacatgaa acgttgacgt attcattaac ttcacctgta atggttgatg
      181 actttaatat ttttcacaca aatgagtctg ttaaattagc aatgccatta ggtgaagaaa
      241 gaagtgtaac aagaaaatct ataattggat cgttattaca agtaatcaat tataatcaat
      301 cacataatat taaggatgtt aatatttttg aattatcaac tacttattct aaaggtgtag
      361 aattacaaaa tcttgcaatt gcctgctgtg gtgaatacaa tggtttaccg tttaaacaaa
      421 tttcatataa agctgattat tatttagtaa aagggtttgt tgaaacaatc tttaaaaatt
      481 tgggaattga agaatctcgt tataaattag ttcgggctag tcaagatgat aagtattatc
      541 atccaggtag aacagcgtat attatgattg gtaaagaagt tgttggggtc gtaggaaata
      601 ttcatccatt gatggaaaag aaatataatg ttaaagatgt ttacattgtt gagttgaatc
      661 taacaacatt attaaattta aaaacaagca aagtgaaatt tactgaaatc ccaatgtacc
      721 cttctgtaag tagagatatt gctttggtga tggatcagga tattcctact tttgatattt
      781 gtcgtaagat tgttcaagcg agcaaacagc tagttaaaga aacaaaaatt tttgatgttt
      841 atgcaggtga gcatattgaa gctgggaaga aatcagttgc aattaacttg cttttccaag
      901 atccaaaagg aactctagaa gaagcaacag ttaatgctgc aatggagaaa attttagcag
      961 ctgtggaaaa agattttgga gcagtgctta gagcataata aaaataaatt agcgaagaaa
     1021 tagacgtatt tgtttatttc ttttttttaa tatgtaattc atattatttg acaaggattg
     1081 gaaccatgat tagaatagag ctaaagaggt ggttatagtg caaaaaatta cattagaaat
     1141 tgatggaatg atgtgtggaa tgtgtgaaag ccatattaac gatgctatta gaaaggcatt
     1201 ttcagtgaag aaagtcagtt cctctcatag taaagataga acagagatca ttactgctga
     1261 tgaacttgat gaggataaat taaaagcggt tatcgatgct actggctata aagttacatc
     1321 gattacaaaa gctccatatg taaaaaaagg tttttttgca tcattccgta aataataaag
     1381 aaatcaggat aaacctgatt tcttttcata gtaggagtat agttttcttg ctagttcgct
     1441 cattctttgc tgattgactt tacagatttt ccgatcataa gtatataggc cattgatttc
     1501 gttttcaata tcagatattt gagtataaat acaaccgcaa agtccattat taattgaggg
     1561 tataaccatt tcttggtaca ttttttcgat tttatcggtt agttcttttt cgctatttgt
     1621 tttaccatag ccatattgtt ttttattgct gttaagatga ttattgattg gccgagtata
     1681 gccgccacat tcacttaata atagaggacg ttcaaggttt gttgacaaga ttttatttcg
     1741 aaaataaata tgacgactga caacatcgct atttttttga ctaaaccacc ctgaggttgt
     1801 atcataaaat cttgttggat ccaactcttt acaaatttga tacatttcat cactattaaa
     1861 ttgaccccag ccttcattaa aaattgtata actaatgata ctagggtgat tatatagatg
     1921 gctgattgtg tcgatcatat gttgcttaaa aatattttgt tgtagggtat ttttgtaacg
     1981 tgtgtcttta aaatttataa accctaaagt tggaaggacg gtatcatgga taaaacggta
     2041 ttcaccatta ttaaccatat cctgcatcac aagaatgcct aatttatcac aattataata
     2101 aaatagctct ggctcgatct taatatgttt acgaataaga ttgaatccca gtttttttat
     2161 attcataatc tcagttttgt aaaaatcagg atgttccggt aaaaataaac catcatgata
     2221 atatccttga tctaagatac catgcaaaaa aatgggagta tcatttaata gaatttgatg
     2281 tttttttatt tcaactgttc ttaaggcaaa gtaactttca atataatcat catcagtggt
     2341 tatagaaaaa gtatataaat aaggatcatc gggagtccaa taatgagggt tatcaatggt
     2401 gatatcagta tcttttgcag tgtaagtctt tttaaaatca ttattttcaa tagtaacagt
     2461 atatcgactg gcctcagtat ttacttttaa attaatgccg gttaatgagg gggtaattaa
     2521 cagatcttta atacaatttt ttgaaacggc ttctacccag actgtctgcc aaattccgga
     2581 tacttgagta taccacattc catggctttc acgactttgt ttaccgtagg ggtaatcatg
     2641 cgataaggag tctgtaactt tcaccactaa tttatttacg ccatcattaa gtgatgaaga
     2701 tatatcaaaa taaaaaggta aatatcctcc tgagtgagaa ccaatatatc gattatttac
     2761 atatacttta gtaagttgat ctacagcacc aaaatggagg agaacctggt tatttgcatg
     2821 atagaagtct ataggtaaaa tgaaatcctt ttggtatact aatgtatcat ttattattcc
     2881 atgataatta gatagtggtg cttgaggagg atagggaata ttaatactga gatcatttaa
     2941 ttgccaaact ccatttaaac tgaaaaaact atttcttttt aattgaggtc gtggatattc
     3001 catagttaac acctgctttc tttactttaa tgatacaggg atactggctt cattgcaatt
     3061 ctagttagaa tttattaaat t
//
LOCUS       DAOMTA010000311         3066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1303_length_3066_cov_2.486217, whole genome shotgun sequence.
ACCESSION   DAOMTA010000311 DAOMTA010000000
VERSION     DAOMTA010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_3066_cov_2.486217"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PLK68_08820"
     CDS             <1..1291
                     /locus_tag="PLK68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM90545.1"
                     /translation="DQCFSMQNGQLIGKYRRDKNNHFYSRLVKQHSPLKLYQLALLQY
                     RANLGRNLKISSGITVALICIMITFTLSGSLQTQIKRQLDSIFPNQLISIQNVHKKLL
                     SYHELLSLSNLDMNEFIYGEPADYEFMGVSLQSDYEVEKTIYISDMTKPIKSRNLEIG
                     RETKNSNEIVLSKTTATHLNPDYQKLLNQQLYGYYLKDDQIKGVSLTVVGIGKDVTAF
                     DTIYINELANLDHISQAFAIDKKQLLFQLAMINLNSKADLDDLLMDLEDQYSQFEFKV
                     AGENINERIDTFMLQIQRVLLLFSLLAIVAACFLIGEVLYLSVIEKTKDIGIFKCMGA
                     SKLQIMNLVLLESFTLISGAFICSYVFFYQLVNLINQLVENELQLDLSGAFIQIDYQL
                     VIAIYLGALCFGLCSSYIPAFLAGRLDPIKALKQPNY"
     misc_binding    1343..1550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1587..>2286
                     /locus_tag="PLK68_08825"
     CDS             1587..>2286
                     /locus_tag="PLK68_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM90546.1"
                     /translation="MPFDHKQIEPKWQKYWDEHKTFKTDCYDDSKPKYYCVDMFPYPS
                     GNGLHVGHPEGYTATDIVSRMKRMQGYNVLHPMGFDSFGLPAEQFAIQTGHHPAEFTK
                     KNIEVFKGQIKSLGFSYDWDREIATSDPEYYKWTQWIFTKLYDAGLAYVDEIPVNWCP
                     ELKAVLANEEVIDGKSERGGYPVIRKPMRQWVLKITEYAERLLEDLDDLDWPEATKQM
                     QRNWIGKSVGANVDF"
     assembly_gap    2287..2296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2297..>3066
                     /locus_tag="PLK68_08830"
     CDS             <2297..>3066
                     /locus_tag="PLK68_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM90547.1"
                     /translation="IDGTDKIFTVFTTRCDTLFGATYCVMAPEHPYVAEITTLEQKEA
                     IEAYKESCASKSDLERTELNKDKTGVFTGAYAINPVNGKKIPIWISDYVLASYGTGAI
                     MAVPAHDDRDWEFAKKFGIEIIPVLEGGNIEEAAYTEDGLHINSQWLDGLGKQEAIDK
                     MIAWLEEHKCGEKKISYKLRDWLFSRQRYWGEPIPIVHMEDGTMRTVPVEELPLELPA
                     TKNFQPHDSGESPLANCEDWLEVEIDGQKGRRETNTMP"
BASE COUNT         1062 a          396 c          610 g          988 t
ORIGIN      
        1 tgatcaatgc ttttctatgc aaaatggaca attgattggg aagtatcgcc gggataaaaa
       61 taatcatttt tattcgcgtt tagtcaaaca gcattcacca ttaaaattat atcaactggc
      121 attgttacaa tatcgtgcta atttgggtag aaatttaaaa atatcatctg gtattacagt
      181 agctttgatt tgtattatga ttacctttac actgagtgga tcattacaaa cccaaataaa
      241 aagacagctt gattctatat ttccaaatca actaatttcg attcagaatg tacataaaaa
      301 attacttagt tatcatgagt tactttcatt atcaaattta gatatgaatg aatttatcta
      361 tggggaacca gccgattatg aatttatggg agtatcactg caaagtgatt atgaagttga
      421 aaaaacaatt tatatcagtg atatgactaa accaataaaa agtaggaatt tggaaatagg
      481 acgtgaaaca aaaaatagta atgaaatagt tttatcaaaa acaacggcaa cacatcttaa
      541 tcctgattat caaaaattat tgaatcaaca attatatgga tattatttaa aggacgatca
      601 aattaaagga gtctcactca ctgttgttgg aatagggaaa gatgtaactg cttttgacac
      661 aatctatatt aatgaattag ctaatctcga tcatattagc caagcttttg cgattgataa
      721 gaaacaatta ttgtttcaat tagctatgat caatttaaat agcaaagcgg atcttgatga
      781 tcttttaatg gatttagaag atcagtatag tcaatttgaa tttaaagttg caggagaaaa
      841 tattaatgaa agaatagata catttatgtt acaaattcaa agagttcttt tactattttc
      901 tttattggct atagtagcag catgtttctt aattggggaa gtgttatatt tgtccgttat
      961 agaaaaaaca aaagatatcg gtatttttaa atgtatgggt gcgagtaaat tacagatcat
     1021 gaatttagtt ttattagaaa gttttacttt aattagtgga gcgtttatct gctcttatgt
     1081 atttttttat caattagtta atttaattaa tcagcttgtt gaaaatgagc tgcagcttga
     1141 tttatcagga gcgtttattc aaattgatta tcagttagta attgctatct atttaggagc
     1201 attatgtttt ggattgtgca gtagctatat tcccgccttt ttggcaggcc ggttagatcc
     1261 tattaaagca ttaaaacaac caaattatta gtaataaaat ttgttgcata tcagcctttt
     1321 tatgttataa tattctagta tttgaaagtc gatgaatagg actagtagta attgacgttt
     1381 tttatagaga atcagtggct ggtggaaact gataaaacgg gttatgaact cacctaggag
     1441 atgcagcggg gaattaagta gtcgttgtcg ttaactgcgt taaagttttg agggctaggt
     1501 cttttagact tggaactaag gtggtaacgc gattaaaagt cgtccttaga taggatgatt
     1561 tttttatttt aaggaggaat tataaaatgc cgtttgatca caaacaaatc gagccgaaat
     1621 ggcaaaaata ttgggatgag cataaaacat ttaaaacaga ctgctacgat gatagtaaac
     1681 caaaatatta ttgtgtggat atgtttccat atccgtcagg gaatggttta catgtagggc
     1741 atccagaagg gtatactgca acagatattg tttcaagaat gaaaagaatg caaggataca
     1801 atgttttaca tccaatggga tttgattcat ttggacttcc tgcagagcag tttgctattc
     1861 aaacaggaca tcatccagct gaatttacta aaaagaatat tgaggtattt aagggacaaa
     1921 ttaaatcttt aggtttctca tatgattggg atagagaaat cgcaactagt gatcctgaat
     1981 attataaatg gacacaatgg atttttacaa aactttatga tgccggtctt gcttatgtag
     2041 atgaaattcc tgtaaactgg tgtccagaac ttaaagctgt tttagcaaat gaagaagtta
     2101 ttgatggaaa aagtgaacgt gggggatatc cggttatccg taagccgatg cggcaatggg
     2161 ttttaaaaat tactgaatat gctgaaagat tgcttgaaga tttagatgat cttgattggc
     2221 ctgaagcaac aaaacaaatg cagcgaaatt ggattggaaa atcagttgga gctaatgttg
     2281 attttannnn nnnnnngatt gatggaacag ataaaatatt tacagtcttt acaactcgtt
     2341 gcgacacttt atttggtgca acttattgtg ttatggcacc tgagcatcca tatgtagctg
     2401 aaattactac attggaacaa aaagaagcta ttgaagctta taaagaaagt tgtgcaagta
     2461 aatcagattt agaaagaact gaactaaaca aagataaaac aggtgttttt acaggagcgt
     2521 atgcaattaa tcctgttaat ggtaagaaaa ttcctatctg gatttctgat tatgtactag
     2581 caagttatgg aactggtgca attatggcag taccagcaca tgatgatcgc gattgggaat
     2641 ttgctaaaaa gtttggaatt gagatcatac ctgttttaga aggcggaaat attgaagaag
     2701 ccgcttatac tgaagatgga ttacatatta attctcaatg gttagatgga ctaggaaaac
     2761 aagaagcgat tgataagatg attgcctggt tagaagagca taagtgtgga gaaaagaaaa
     2821 tttcttataa gttaagagac tggttatttt ctcgtcaacg ttattggggt gagccaattc
     2881 caatcgtaca tatggaagat ggaacaatga gaaccgttcc ggttgaagaa ttaccattag
     2941 aattgccagc tactaagaat tttcaacccc atgatagtgg tgaatcacca ttagctaact
     3001 gtgaagattg gttggaagta gagattgatg gtcaaaaagg tcgtcgtgaa actaatacaa
     3061 tgcctc
//
LOCUS       DAOMTA010000312         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1307_length_3061_cov_2.389887, whole genome shotgun sequence.
ACCESSION   DAOMTA010000312 DAOMTA010000000
VERSION     DAOMTA010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1307_length_3061_cov_2.389887"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLK68_08835"
     CDS             <1..465
                     /locus_tag="PLK68_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRM90548.1"
                     /translation="LPILMSGIQLLCLFMMQMDPKYKNYSSKMLKIVVWIIPTITIVL
                     EAITYAIVFGYEISINIVVPIFLGILFVILGNYLPKCRQNFTMGYRLPWTLNDEDNWN
                     KTNRLAGYVMVLGGILIIIMSFFDLAFWAVILFVGAILIIPSVYSFLLYRKK"
     gene            574..831
                     /locus_tag="PLK68_08840"
     CDS             574..831
                     /locus_tag="PLK68_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRM90549.1"
                     /translation="MGKLEDLPNIGKVVAQQLIQVGIDDPIKLKEVGAKEAWLKIQQI
                     DESACIHRLYALEGAVEGIKKTQLDYETKAELKQFYNEHKL"
     gene            828..1487
                     /locus_tag="PLK68_08845"
     CDS             828..1487
                     /locus_tag="PLK68_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM90550.1"
                     /translation="MKILVNGIELFYEISGQGRPLILVHGNGEDHHIFDALVAKLKNH
                     YTCYCIDSRGHGLSSSVAVYSYQSMAEDIIEFIKSLKLSEVAYYGFSDGGIVGLLVAS
                     QSKLIKYLMISGANADPDGLKNWAYYLMKIQYYLKKDVKIKMMLEQPHINEYQLAKIT
                     AKTLILVGSDDMIKESHTLYLAHNISGSQLMILPNENHSSYIINSAKLAPIILAFLKV
                     A"
     gene            1833..>3061
                     /locus_tag="PLK68_08850"
     CDS             1833..>3061
                     /locus_tag="PLK68_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM90551.1"
                     /translation="MFSNKALKKLILPLIIEQILIMAVGVADTVMVSYAGEVAISGVG
                     LVDMFNNLIITVLAAIDAGGAIIVSQYIGNKDRKNANKASSQLLTITIVIATVIMLGC
                     LVFHRILLSTFFGAIEMDVMKAATTYFLISAISFPFLGVYNSAAALYRSMEKTRTTMY
                     VSILMNIINVVGNYIGVFILHAGVAGVAVPTLISRIVAAIIMFALSLNSSNLVYVKIK
                     NVFAWDQEMISRILKIAVPNGIENGLFTLGRVLVTSIVALFGTSQIAANSVAGSIDQI
                     AVVVVNAINLGIVVVVGQCIGANDYEQAKYYIKKLMKISYIVTGIIGSAVILLLPWIL
                     NLYSLSSEARNLTFILVIMHNIMATALHPTAFVLPNGLRAAGDVKFSMVVGIVSMILF
                     RLGAAVLFGIIFNLGIIG"
BASE COUNT         1027 a          337 c          607 g         1090 t
ORIGIN      
        1 ttaccgatat tgatgagtgg aattcaacta ttgtgcttgt ttatgatgca aatggatcct
       61 aaatacaaga attattcatc taagatgtta aaaattgttg tttggataat tccaacgata
      121 acaattgttt tagaagcaat aacatatgca atcgtatttg gatatgaaat ctcaattaat
      181 atagttgttc caatattttt aggaatatta tttgttattt tagggaatta tttacctaaa
      241 tgtcgtcaaa attttactat gggctaccga ttaccatgga ctttgaatga cgaggataat
      301 tggaataaaa caaatcgttt agctgggtat gtaatggtcc ttgggggtat tttaattatt
      361 attatgagtt tctttgatct ggctttttgg gcagtaattt tgtttgttgg agcgatttta
      421 ataatccctt cagtgtattc atttttactc tatcgaaaaa aataataaaa tagatgattc
      481 gatacatttt tatttaaaga attaaataga aatgtatctt tttagatgct ttgttttttt
      541 atttgaagta caataagagt aggagatgat aatatgggaa aattggagga tttaccaaat
      601 attggtaagg tagtagcaca acagctgata caagtaggaa ttgatgatcc gataaaactg
      661 aaagaagttg gagcaaaaga ggcatggtta aaaatacagc aaatcgatga atctgcatgt
      721 attcatcgct tatatgcact agagggggca gttgagggaa ttaaaaaaac gcaattagat
      781 tatgaaacga aagcagaatt aaagcaattt tataatgagc ataagttatg aaaatattag
      841 ttaatggtat tgagttattt tatgaaatat cgggtcaggg acggccgctt attttagttc
      901 atggtaacgg tgaggatcat catatttttg atgcattagt ggccaaattg aaaaatcact
      961 atacttgtta ttgtattgac agtcgcgggc atggtttaag ttctagtgta gctgtatata
     1021 gttatcaatc aatggctgag gacataattg agtttattaa aagcttaaaa ttatcggagg
     1081 ttgcctatta tggttttagt gatgggggaa tcgtcggatt attagtagcg agccaaagta
     1141 aactgataaa atatttaatg attagtggtg ctaatgctga ccctgatggc ttaaaaaatt
     1201 gggcttatta tttgatgaaa atacagtatt atttaaaaaa agatgtaaag attaaaatga
     1261 tgctggaaca accacatatt aatgaatatc aattggctaa gataactgca aagacgctaa
     1321 ttttggtagg aagtgatgat atgatcaaag aatctcatac gttatatctc gctcacaata
     1381 taagtggaag tcaactaatg atcttgccta atgaaaatca tagtagttat attattaata
     1441 gtgcaaaact tgcgccaatt attttggcat ttttaaaagt cgcttaatga ggtgaataca
     1501 attttttgga cgctaagtta gttatattat taataaggat tgattcttgt taagtagaca
     1561 aatgaaataa ataaaaagtg tgagatgttt tactgatttg gtgaaacgtc ttttttatat
     1621 cagtatataa ttattattac aaagataaca gttattgcat ggtttatttt attgaatatg
     1681 ggataaagaa gattaaaggt atcaaagata atatttacta gttttgctat ttataatgct
     1741 ttaaaggtat catactgata gatgataggt attagacatt ataatattcc taatatacaa
     1801 tgtttttgta aagaaaaagg gaggtagaaa caatgttttc taataaggca ttaaaaaaac
     1861 taattttacc actgatcatt gaacaaattt taatcatggc agtaggagta gctgatactg
     1921 taatggtatc ttatgcaggt gaggtggcta tttcaggtgt tggattagta gatatgttca
     1981 ataatttaat cattactgta ctagctgcta ttgatgctgg tggagcgata attgtttcac
     2041 aatatattgg aaataaagat cgaaagaatg ctaacaaagc atctagtcag cttttgacga
     2101 ttacaatagt aattgctact gtaattatgc tggggtgtct cgtatttcat cgtatactcc
     2161 ttagtacatt ttttggtgcg attgaaatgg atgttatgaa agcagcaacg acgtattttc
     2221 taatttcagc tatttctttt ccatttttag gtgtgtataa ttcagcagcg gcattatata
     2281 gatcaatgga aaaaacaaga actacgatgt atgtatcaat cttaatgaat attattaatg
     2341 ttgttggaaa ttatattggg gtatttattt tacatgctgg agtagcaggg gtagctgtac
     2401 caacattgat atcaaggatt gttgcagcga ttattatgtt cgcattatca ctaaactcat
     2461 ctaatttagt ttatgtaaag attaaaaacg tatttgcatg ggatcaagaa atgattagta
     2521 gaattttaaa aattgcagta cctaatggaa ttgaaaatgg attatttacg cttggtcggg
     2581 tgttagtaac gagtattgtt gctttatttg ggacaagtca aattgctgct aatagcgttg
     2641 ctggaagtat tgatcaaatt gcagttgttg ttgtaaatgc gattaatttg ggtattgtag
     2701 ttgttgtagg acaatgtatt ggagccaatg attatgagca ggcaaaatac tatataaaaa
     2761 aattaatgaa aatatcctat attgtcacag gaatcattgg cagtgctgtt atattgttat
     2821 tgccgtggat cttgaatcta tattctttat caagtgaagc aagaaattta acttttattt
     2881 tagttattat gcataatatc atggcaacag cattacatcc aactgctttt gttttaccaa
     2941 atggattacg ggctgctggt gatgttaaat tttcaatggt tgttggaatt gtatcgatga
     3001 tcttatttag actaggtgca gccgtgttat ttggaattat ctttaatcta ggaattattg
     3061 g
//
LOCUS       DAOMTA010000313         3060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1308_length_3060_cov_3.013644, whole genome shotgun sequence.
ACCESSION   DAOMTA010000313 DAOMTA010000000
VERSION     DAOMTA010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1308_length_3060_cov_3.013644"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..2296)
                     /locus_tag="PLK68_08855"
     CDS             complement(218..2296)
                     /locus_tag="PLK68_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRM90552.1"
                     /translation="MEMNKLLEDYGCLAFSDDVMKERIPKSIYKAFHESLDNGEELSK
                     ECATVIANAMKIWALENGATHFTHWFMPMTGLTAEKHDAFLEPDGCKAVLEFSGKTLR
                     KGEPDASSFPSGGLRATFEARGYTAWDCTSPAFVKDGTLYIPTLFCSYTGEALDKKTP
                     LLRSCDALSNAACRLLSLLGEKGITKVTASVGAEQEYFLVDDKYYQERMDLKLTGRTL
                     FGAMAPKGQELEDHYFGSLKRKVSAFMKDLDHELWKYGIPSKTKHNEVAPAQHEVACV
                     YSKVNITTDNNHLLMQIMQDIAKKHGLRCLLHEKPFAGVNGSGKHDNWSVITNTGINL
                     FNPGANPAENKPFIATLACTIKAVDDYADLLRMSIASAGNDHRLGANEAPPAIISMFL
                     GEELDALLAEICEGKKTSKSDAARFATGVSVVPTFSKDNTDRNRTSPFAFTGNKFEFR
                     AVGSSQSVAGPNTILNAILADAMEKMADEIESGKSFEDVVKEFVLAHKRIIFNGDGYS
                     AEWEEEAAKRGLPNNKNTVDALKCLKEEKNLEMLDRLGVYSRVELGSRYEILLENYIK
                     TIQVEGLTALKMAKSQIYPAVCDYLSKVSSEVIAAKEAGLDVDFLVDDANALAKLVKT
                     MKEQMTTLETNIAAAQASEEEIFEQAVAWRDDVFAMMQALRETVDQLEESIDAEYWPM
                     PTYLDLLFGI"
     gene            complement(2522..3028)
                     /locus_tag="PLK68_08860"
     CDS             complement(2522..3028)
                     /locus_tag="PLK68_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRM90553.1"
                     /translation="MKEKEFKIKLNPAILYFEIFYMVYVVYLFIIGQMVPAIVCAICG
                     LLIIAYFSLWRPYKYTINRRTLIINRRVGKDKEINIMTCETICDPVPKMTKLITSPRA
                     LEIYAENKKRFVVTPKDRMGFIEAIVAANKRIHVQCTEYAATHRSYEKKRKRALKEEA
                     KKANMDAE"
BASE COUNT          974 a          605 c          388 g         1093 t
ORIGIN      
        1 tgggtgtttt aaaatcattt ccattgtcat ataattacca tttgaacagc ctcaaataat
       61 aattcactaa catgctccaa ataactcatc agcataaata gaattaccaa cttgataatt
      121 attattgcct ccatcctcat tgttgaactt tgtagcacta aaatatatta tatagataaa
      181 aaaatccttt gagatataaa tcccaaagga aatattatta aattccaaat aataaatcta
      241 aatatgttgg cattggccaa tattcagcat caatagattc ttcaagctga tcaactgttt
      301 ctctcaatgc ttgcatcatt gcaaaaacat catcgcgcca tgcaactgcc tgttcaaaaa
      361 tttcttcttc agaagcttga gctgcagcaa tattagtttc taaagttgtc atttgttctt
      421 tcattgtctt cactaattta gctaaagcat tagcatcatc aactaagaaa tcaacatcta
      481 aacctgcttc cttagcagca atcacttcag aagatacttt tgataaataa tcacaaactg
      541 ctggataaat ttgagatttg gccattttta acgctgttaa cccctcaacc tgaatagttt
      601 taatatagtt ttctagtaat atctcataac gagatcctaa ttcaactcta ctataaaccc
      661 ctaaacgatc taacatctct aaattctttt cttctttcaa acattttaaa gcatcaacag
      721 tatttttatt attaggaagt ccacgttttg ctgcttcttc ttcccattct gctgaatatc
      781 catcgccatt aaaaataatt cgtttatgag ctaaaacaaa ttctttaact acatcttcaa
      841 atgatttgcc tgattcaatt tcatcagcca ttttttccat tgcgtcagct aaaattgcat
      901 ttaaaatagt atttggtcca gcaacagatt ggcttgaacc aactgctcta aattcaaact
      961 tatttccagt aaacgcaaat ggtgatgtac gattacgatc tgtattgtct tttgagaaag
     1021 ttggtactac tgatactcca gtggcaaaac gtgccgcatc agatttgcta gttttcttac
     1081 cttcacaaat ttctgctaaa agagcatcta actcttcccc taagaacata gaaataattg
     1141 caggtggtgc ttcatttgct cctaaacggt gatcgtttcc agcactggca atactcatcc
     1201 gtaataaatc agcataatca tcaacagctt taatcgtaca tgcaagcgtt gcaataaatg
     1261 gtttattttc agcaggatta gcacctggat tgaataaatt aatacctgta ttagtaatga
     1321 ctgaccagtt atcatgttta ccagaaccat ttaccccagc aaatggcttt tcatgtaata
     1381 agcagcgtaa accatgcttt ttagcaatat cttgcataat ttgcattaat aaatggttat
     1441 tatcagtagt aatatttact tttgaatata cgcatgctac ttcatgttgt gccggtgcaa
     1501 cttcattatg ttttgttttt gaaggaattc catatttcca taattcatga tctaaatctt
     1561 tcataaaggc agacacttta cgtttaagac taccaaagta atgatcttct aattcttgtc
     1621 ctttcggcgc cattgcccca aatagggtac gtccagttaa ctttaaatcc attctttctt
     1681 ggtaatattt atcatcaact aagaaatatt cttgttcagc cccaacagat gcggtaacct
     1741 tagtaattcc tttttcacca agcaaagaca ataatcgaca tgcagcatta gataatgcat
     1801 cacaagatcg cagcaatggt gtctttttat ctaatgcctc tccagtgtat gaacaaaaca
     1861 aagttggaat atataaagtt ccatctttaa caaatgccgg tgatgtacaa tcccatgcag
     1921 tatagcctct cgcttcaaat gttgcccgta atcctccaga cgggaaagac gatgcatcag
     1981 gttcaccttt tcttaatgtt tttccactaa attctaatac tgctttacaa ccgtcaggtt
     2041 ccaaaaaagc atcatgtttt tcagctgtca aaccagtcat tggcataaac caatgggtaa
     2101 aatgtgttgc accattttct aatgcccaga ttttcattgc attagcaatt actgttgcgc
     2161 attctttaga taattcttca ccattatcta aagactcatg gaatgccttg taaatagatt
     2221 taggaatccg ttctttcatt acatcatcac taaatgccaa gcatccgtaa tcttctaata
     2281 acttattcat ttccattcta agttccccct tgttagtttc taaacaacac ataaccatac
     2341 acttaatata tgtttatatg ccgttattat acagttactt tatttaggtg tcaatatcta
     2401 tatgatattt ttttatcatt ttgttaattc tttattaaaa tgataaatta tttataactg
     2461 tttacttaaa gatacttatt acaactaagt aatattccat ttttacaaaa gaaaaagatg
     2521 cttattcagc atccatattt gcttttttag cttcttcttt caaagctctt ttgcgtttct
     2581 tttcatatga tctatgagta gctgcatatt cagtacattg cacatgaatt cttttattag
     2641 cagctacaat cgcttcaata aatcccattc tatcttttgg tgttacaaca aatctttttt
     2701 tattttcagc ataaatttct aatgcccgtg gactagttat aagttttgtc atctttggta
     2761 caggatcaca aattgtttca catgtcataa tattaatttc tttatcttta ccaacacggc
     2821 gattaattat caatgttcta cgattaattg tatatttata tggtcgccat aaagaaaaat
     2881 atgcaataat taataatcca caaatagcac acactatcgc aggtaccatt tgtccaatta
     2941 taaataaata aactacatat accatataaa agatttcaaa gtatagtatt gcgggattca
     3001 atttaatttt aaactctttt tctttcataa taacctctct ttttcctcaa cttcatcatt
//
LOCUS       DAOMTA010000314         3059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1309_length_3059_cov_2.723369, whole genome shotgun sequence.
ACCESSION   DAOMTA010000314 DAOMTA010000000
VERSION     DAOMTA010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_3059_cov_2.723369"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PLK68_08865"
     CDS             complement(<1..1440)
                     /locus_tag="PLK68_08865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM90554.1"
                     /translation="MGFDDFKQLVDNMQINLYLTDIQTNEIIFMNNKMKETYNLKDPE
                     GKICWKTLQKNQSGPCPFCPLIKLKQINNLYQSISWRENSDLTNRIFENYDSLIKWEN
                     GKIVHMQQSIDITDSEILSHQASIDDLCGLLNRRAGKEKLLEFMVKKKKAKESFTIAL
                     LDINNLKRVNDSFGHQEGDFLLKQIAYAVKQDLDKEDLIFRLGGDEFVLVFGHKNTKD
                     SAKFLIENQKNIQKLQALYNKPYGFSYAFGMYTVLPTNKLTVNDVITRADEQMYQEKL
                     RYRRTQLAELEDVSVGSTNVACFQYDPTQLYEALIKSTDDFIYICNMKTGIFRYSPAQ
                     VKVFNLPGEIIDHPLPFWKAIVHPQDWERFYKSNMAIGENKMDYHSVEFRAMNSDGEY
                     IWLKCRGQLMRDEFGEPNLFAGIMTQLDRQNKIDPLTHLYNRQEFAKAFELKTKDKAI
                     DNLGIMVIDIDDFKNINEIYDRSFGDFIIK"
     gene            1672..2043
                     /locus_tag="PLK68_08870"
     CDS             1672..2043
                     /locus_tag="PLK68_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90555.1"
                     /translation="MKEDLIIEWFQSWFDSHWNNFEVIFESDAYYSESWGPEYKGIIE
                     IKKWFNDWHEHFKLDKWEIKQFIHTNKHSIVEWHFSCIDLDGVHEFDGISLIEWSPNS
                     LIKSLKEFGSSLPKYDPFEQL"
     gene            2065..3033
                     /locus_tag="PLK68_08875"
     CDS             2065..3033
                     /locus_tag="PLK68_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM90556.1"
                     /translation="MFYKIYHDTIPPYLLEIMSTNIFTRLKDIGMNCGMEYTQFQTFK
                     NSFGVSRYDHSIGVSLITYHFTHDKHATVAALLHDIATPVFAHVIDFLNNDYEKQEST
                     EAETKKMIFNSRELQKIFLKYEIDINLIYDYHKYPIADNESPKLSADRLEYTLANAYY
                     YNFLSVEQIKSIYYDLIINDKKDEIIFKSKDKAIMLTKVMFQCSRVYTSDENRYCMEY
                     LANLLKTALTKKLITQDDLYTNESSVIKNICKDKELSDKWEAFCHFHQVDISHNKKAG
                     YYKINAKHRYFNPMIGNQRIINQSPKFKSELNSFLGDHFDRYVKVT"
BASE COUNT         1099 a          475 c          399 g         1086 t
ORIGIN      
        1 tttaataata aaatctccaa atgaacggtc atatatttcg ttaatatttt taaagtcatc
       61 aatatcaata accataattc caagattatc gattgcttta tctttagtct taagctcaaa
      121 agctttcgca aattcttgac gattatataa atgagttagg ggatcaattt tattttgacg
      181 gtctaactgt gtcattattc cagcaaataa attaggctca ccaaattcat cgcgcattaa
      241 ctgtccacga cattttaacc aaatatactc accatcacta ttcatcgctc gaaattcaac
      301 agaatgataa tccatcttat tttcgccaat tgccatattt gatttataaa aacgttccca
      361 atcttgggga tgtacgattg ccttccaaaa tggtaaagga tggtcaataa tttctcctgg
      421 taaattaaaa acttttactt gggctggtga atatctaaaa atacctgtct tcatattaca
      481 tatgtaaata aaatcatcgg tacttttgat caaagcttca taaagttgtg ttggatcata
      541 ttgaaaacaa gctacattag tagatcctac tgaaacatct tccaactctg ctaactgagt
      601 tcgacgatat cgcaactttt cctgatacat ttgttcatct gctctagtta tcacatcatt
      661 aacagtcaat ttattagttg gtaaaactgt atacattcca aaagcatatg aaaatccgta
      721 aggcttattg tataaagctt gcagcttttg tatatttttt tgattctcaa ttaaaaattt
      781 agcactatcc ttagtatttt tatgcccaaa aactagaaca aattcatctc cacctaatct
      841 aaagataaga tcttccttat caagatcctg ctttactgca taagcaattt gttttaataa
      901 aaaatctcct tcttgatgac caaaagaatc atttactctt tttaaattat ttatatctaa
      961 taaagcaatt gtaaagcttt ctttagcttt tttctttttc accataaact cgagcagttt
     1021 ttcttttcca gcacgacgat ttaataatcc acataagtca tcaatactag cctggtgact
     1081 taggatttca gaatcagtaa tatcgattga ctgttgcatg tgaacaattt taccattttc
     1141 ccatttaatt aaactatcat agttttcaaa aatacgatta gtaagatcac tattttcacg
     1201 ccagctaatc gactgatata aattattaat ttgttttaat ttgataagtg gacaaaatgg
     1261 acatggtccg ctttgattct tttgaagtgt tttccaacat attttacctt ctggatcttt
     1321 caaattataa gtttctttca tcttattatt cataaaaatt atttcatttg tttgaatatc
     1381 tgtaagatat aaattaattt gcatattatc taccagctgc ttaaaatcat caaaacccat
     1441 tgttaacact cctttactcc aaatttcaac tatctttatt ataccatact aacaattact
     1501 aaaggtaaaa actattttta aattataaaa aacattagtt atcattaaat ataaaacttt
     1561 atccccaaaa actttgaaga tatttgcttt ggacttagat aagaaaatga cgagtatcac
     1621 aaataatgct atactttaag tactattaat aatttagaaa ggataatttt tatgaaagaa
     1681 gatctaataa ttgaatggtt tcaatcatgg tttgattccc attggaataa ttttgaagtg
     1741 atttttgaat ccgatgctta ttatagtgaa tcttggggtc ctgaatacaa aggaatcatt
     1801 gaaataaaaa aatggttcaa tgactggcac gaacacttta aattagataa gtgggaaatt
     1861 aaacaattta ttcacactaa taaacattct attgttgaat ggcactttag ttgtattgac
     1921 ttagatggcg ttcatgaatt tgatggtata tctttgattg aatggagtcc taatagtctt
     1981 ataaaatcat taaaagaatt tggatcatca cttcctaaat atgatccctt tgagcaacta
     2041 taattaacac agggagagat aataatgttt tataaaatat atcatgatac gattccaccg
     2101 tatcttttag aaatcatgag cacaaatatc tttactcgcc tcaaagatat tggaatgaat
     2161 tgtggaatgg aatatacaca gtttcaaaca tttaaaaata gttttggagt ttcaagatat
     2221 gatcatagta ttggcgtttc tttaataact tatcatttta ctcatgataa gcatgctact
     2281 gttgcagctc tattacatga tatagccacc cctgtattcg ctcatgttat tgacttcttg
     2341 aataatgatt atgaaaaaca agagtctact gaagctgaaa caaaaaagat gatttttaat
     2401 agtcgtgaat tacaaaaaat ttttcttaaa tacgagatag atattaatct aatctatgat
     2461 tatcataaat atccaattgc cgataatgaa agtcctaaat tatcagctga tcgattagaa
     2521 tatactcttg caaacgcata ttattataat tttttatcag tcgaacaaat taagtcaatt
     2581 tattacgatt taataattaa tgataaaaaa gatgaaatta tctttaaaag taaagataaa
     2641 gcaataatgt tgacaaaagt aatgtttcaa tgcagtcgtg tttacacctc tgatgaaaat
     2701 cgatattgta tggaatattt agctaatcta ttaaaaactg cgttgacaaa aaagcttatc
     2761 acacaagatg atttatatac taatgaaagt tctgtcatta aaaacatctg caaagacaaa
     2821 gaattaagcg ataaatggga agctttttgt cattttcatc aagttgatat tagtcataat
     2881 aaaaaagcag gttattataa aataaatgct aaacatcgct actttaatcc tatgattgga
     2941 aatcagcgaa tcatcaacca gtctcccaaa tttaaaagtg aactaaacag ttttttagga
     3001 gatcattttg atcgttatgt taaagttact taaaataagt tattctcaaa aaaaacaac
//
LOCUS       DAOMTA010000315         3058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1311_length_3058_cov_2.243756, whole genome shotgun sequence.
ACCESSION   DAOMTA010000315 DAOMTA010000000
VERSION     DAOMTA010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_3058_cov_2.243756"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>413)
                     /locus_tag="PLK68_08880"
     CDS             complement(24..>413)
                     /locus_tag="PLK68_08880"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRM90557.1"
                     /translation="ALNINDKRSAQKQAMYRNIDSALLPAGESLKTTIERVIPYFNET
                     VKKDMQAGKRALIVAHGNSLRALVKYFDKLSNEAIMNINIPTGIPLVYEFDDEFKVIK
                     HYYLGDETLLKEKIDAVADQGKKLVTV"
     assembly_gap    414..423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<424..784)
                     /locus_tag="PLK68_08885"
     CDS             complement(<424..784)
                     /locus_tag="PLK68_08885"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRM90558.1"
                     /translation="MKLVLVRHGESDWNKLNLFTGWTDVDLSQTGHREAIQAGTILKN
                     EGYEFDVCYTSYLKRAIHTLNHILDEMDLCWLPVNKSWKLNERHYGALQGLNKAETAE
                     KYGEEQVKIWRRSFDVLP"
     gene            1214..2404
                     /locus_tag="PLK68_08890"
     CDS             1214..2404
                     /locus_tag="PLK68_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM90559.1"
                     /translation="MSRVKSLNIKYVASQVFYFATFAAMMGYASVYLLHKGFSNSTIG
                     IILSLCNILAVFMQPALATFADKNQKIEIRKIITTIVAIAIALSAILLVVPSNQAIIF
                     ILIVSIFSLMTTIMPLMNTLAFVFEKYGIQVNYGLARGLGSVAYAVASMVLGHAVDAF
                     SPDLLPVCYVVFNALLFIVVHGYVLPKSEQIEVAVETNDEDEVAVNNEGLLRFAGKYK
                     KFIVFLLGFVFVYFAHTIINNFFIQIITNVGGNSSDMGNAVFLAAMLELPTMAYFTKL
                     SKKVNCGTLIKISIILFLVKHVITYFADGMTMIYIAQAFQMGAYALFIPASVYYVNCK
                     IAPQDMVKGQSFVTTSMTVAGVFGNLIGGMLLDSVGVSQVLLISAVLSLIGAVIVVMS
                     VEKV"
     gene            complement(2693..>3058)
                     /locus_tag="PLK68_08895"
     CDS             complement(2693..>3058)
                     /locus_tag="PLK68_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM90560.1"
                     /translation="KTILCIDSFRVLKKITVCLNSVRKRILRRYKDSQEYKLLKYRYE
                     LLLKSGDKINDEKYFFDRTLGYTTSEAGVLECVLSINKELKMAYNLKEDYRVFNDVKE
                     NEYNEEEYLTDVLKEKIIM"
BASE COUNT          964 a          433 c          483 g         1168 t
ORIGIN      
        1 aaggatcaat gtttcaatca taattaaaca gtaactagtt ttttaccctg atcagcaaca
       61 gcatcgatct tctcttttaa tagcgtttca tcacctaaat agtaatgttt tattacttta
      121 aattcatcat caaattcata tactaaagga attccagttg gaatattaat attcatgatt
      181 gcttcgttac ttaacttatc aaagtattta accaacgctc ttaatgaatt accatgtgca
      241 acaattaagg ctcgtttacc agcctgcata tcttttttta ctgtttcatt aaaataagga
      301 ataacccgct caattgttgt ttttaagctc tcacctgctg gtaaaagagc agagtcaata
      361 tttcgataca ttgcctgctt ttgcgcgctg cgtttatcat ttatatttaa agcnnnnnnn
      421 nnngtggcag tacatcaaat gaacgacgcc aaattttcac ctgttcttca ccgtattttt
      481 ctgcagtttc cgctttattt aatccttgta aagcaccata atgacgttcg tttagtttcc
      541 agcttttatt gacaggcagc cagcagagat ccatttcgtc taatatatga tttaatgtgt
      601 gaatggctct tttcagataa gaagtgtagc acacatcaaa ttcataacct tcatttttta
      661 aaatcgtgcc tgcttgtatt gcttctcgat ggccagtttg gcttaaatct acatctgtcc
      721 atcctgtaaa aagatttaat ttattccagt cactttctcc atgacgtact aatactagtt
      781 tcataatatc tctcctttta gttagtttaa gctaactata tttgtataat attccaatga
      841 acaaaatttg tcaactaaga tgataataaa agcaagctgt tacaatattt ttcacaaaag
      901 aattaatctg attttactga ctgaaaagat taagatttca tttgaaatga tttttagtta
      961 taataatttt ataggcttga gaagttagag agtgagggaa tggttatagt aacagcaagc
     1021 actgatgtgg ctttttgatt atcaatgaaa atatatataa aaatatgata atattttagt
     1081 aacattggaa ataaaaatgc cggtatcgat taatattgat gaaagcgact tttcaacttt
     1141 tgaaaatgct ggatttaata ttaaaaataa tgatatatta gaaataatga aagcgattta
     1201 atgaggtagg aaaatgagta gagtaaaaag tttaaatatc aagtatgtag ccagtcaagt
     1261 attttatttt gcaacctttg ccgcaatgat gggatatgcc tcagtatacc tattgcacaa
     1321 aggttttagt aattcaacaa ttggtataat tttgtcccta tgcaatattt tagcggtgtt
     1381 catgcaaccg gcattagcaa cttttgctga taagaatcaa aagattgaaa ttcgtaaaat
     1441 tattacaact atcgttgcaa tagcaattgc tttatctgca attttattag tagttccaag
     1501 taatcaggca attattttta ttttgattgt atcaatcttt tcattaatga caacgataat
     1561 gccgctaatg aatactttgg catttgtttt tgaaaagtat ggaattcaag tgaattatgg
     1621 cttggctcga gggctggggt ctgttgctta cgctgttgca tcaatggtat taggacatgc
     1681 agttgatgct tttagtccgg atttattacc agtatgttat gttgtcttta atgcactatt
     1741 atttattgtt gttcatggct atgtattgcc aaaaagtgaa caaatcgaag ttgcagttga
     1801 gacaaatgac gaggatgaag ttgcagtaaa taatgaaggt ttgctaagat ttgctggtaa
     1861 atataaaaaa tttattgtat ttttattagg gtttgttttt gtttattttg cccatacaat
     1921 tattaataat ttctttatcc aaataattac taatgtaggc ggtaatagta gtgatatggg
     1981 aaatgctgta tttttagcag cgatgttaga attaccgaca atggcatatt ttacgaagct
     2041 tagtaaaaaa gttaattgtg gaacgttaat taaaatttct attattctat tcttagtaaa
     2101 acatgtgatt acttattttg cagatggaat gacaatgatc tatatagctc aagctttcca
     2161 aatgggggct tatgctttat ttattccggc atctgtttat tatgtgaatt gtaaaattgc
     2221 cccacaagat atggttaaag gtcaatcatt tgtgactact tcaatgacag tagcaggggt
     2281 ttttggtaac ttgattggtg ggatgttact agattcagtt ggtgttagcc aagtactgtt
     2341 aattagtgct gttctatcat taataggtgc ggtaattgta gtaatgtctg ttgaaaaagt
     2401 ataattttat agaattaatg aaagtgttgg tttagttatt taaaatggtg ctttaaaata
     2461 atatagggta ctaaacaaaa agtaggatta gaagatgtat aatcttcaat aattatccta
     2521 cttttttatg tctagatata gtattatttc attttttatt tgtttttgtt tttcttgatt
     2581 tgtttcttat taatagaaat tgtatacgta tctaaaagat tctgagaata cataattcta
     2641 tttctagctc tgtcaaaatt ggtcattcca ttttcattag atagaatctt ttttacataa
     2701 ttattttttc cttcaataca tccgttagat attcttcttc attatattca ttttctttaa
     2761 catcattaaa tacacgataa tcttctttca aattataagc catttttaat tctttgttta
     2821 tagaaagaac acattctaat actcctgctt cactagttgt ataacctaat gtacgatcaa
     2881 aaaagtactt ttcatcattt attttatcgc cactctttaa aagcagttcg tatctatatt
     2941 ttaataattt gtattcttga ctatctttgt aacgtcttaa tatccttttc ctaactgaat
     3001 ttagacagac tgtgatcttc tttaaaacac ggaaactgtc aatacatagt atagtttt
//
LOCUS       DAOMTA010000316         3057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1314_length_3057_cov_2.513991, whole genome shotgun sequence.
ACCESSION   DAOMTA010000316 DAOMTA010000000
VERSION     DAOMTA010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1314_length_3057_cov_2.513991"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="PLK68_08900"
     CDS             <1..1653
                     /locus_tag="PLK68_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90561.1"
                     /translation="GVISLGIGLLLGIGLSQLLSIFTAKLFEADMTKFTFVFSSGALL
                     SAIINFGFIYIIVMIFNIITLNRFKLIDLLYANRKNEKVKIKNGWLIFIIFIISIVLI
                     GYAYKLLYDGALYVVGSDFLIMIILGTIGTFLFFLSVSGFLLKVVQMRKKTYYNGLNM
                     FVLKQVNNKINTHVISTTVISLMLLLTIGILSSSLAMASAMNTTYEGNSPSDVTVFSS
                     NTEIIERFKQEPVYQQIIKEDYQFNYLNLQGLKQGIFIDSKDNAYTNIEAVKNQPMRV
                     IKESDFNKIMELNGKEDQKIQLANNQYELVATYPVALEYYNKFLAGDSTIKIGDTTLQ
                     SNTDQAVELAITNDSGNEGFVVVNDQIANKYVEEQESYLTYLVADYQGDKEECEEQFQ
                     DLLKSFNEQLRTESSPTVISFTRIGLGQSGIGTSALFTFVGLYLGIVFALASGTVLAI
                     EQLSESSDNKERYRILGQLGASKPMVNRSLLVQIGITFMFPLIVALIHSFVALKEINY
                     IISLMVSINIADNILVTTIFIVIVYGGYYLATYLASNRIINE"
     gene            complement(1673..>3057)
                     /locus_tag="PLK68_08905"
     CDS             complement(1673..>3057)
                     /locus_tag="PLK68_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90562.1"
                     /translation="KQIYQEIVAYYNGSIQNVLDILCYDEIVFLQNFEKTKSYRKDIP
                     TINTLINKCLLIKNPNNNNYLEIPEDIKINVINAVEQADIDKVIQIDQINELLIGILA
                     IRGIIEPQELVNIYHSYDPSLSRNEVNTHLDTNHYLFQHYFIYQGETELLLAYEPYRP
                     IIQKIEQLQTLVKKTPAAYTAKQLRTIAKYGFDLSEPAITNLYEAVEQIDNLFVRELF
                     RDSIMLFCQIHGDLNDLIYMINELKITNNQVIEHLEKNLRAAVPLIHSAAFYGLSPYD
                     YYLTINKDSYFSEQESSTFYQLYLSLLEYTNQVFKITDISMKNLDYIEQDDFSQIRTL
                     LFDNPNIIDHYISENPEHLTNYELTIINDFKAGFIDDFLILTNLDDYTLVSNETGVYA
                     IYGLVNHLKEIYPNSTLPQVCNLALLPYRHKIVFDGLLEDRQSILPHKQIRTYSHDDI
                     IYELPHTLLN"
BASE COUNT         1042 a          388 c          530 g         1097 t
ORIGIN      
        1 ggagtgattt cattaggaat cggtttactc ttaggaattg gcttatctca attgttatca
       61 atttttactg caaaattgtt tgaagctgat atgaccaaat ttacatttgt tttttctagt
      121 ggagcacttt tatcagcgat tattaatttt ggatttattt atattattgt aatgattttc
      181 aatattatca cactaaatag atttaagttg attgatttat tatatgcaaa tcgcaaaaat
      241 gaaaaggtta aaataaaaaa tggctggtta atttttatta tttttattat ctctattgtt
      301 ttgattgggt acgcatataa gttattgtat gatggcgctt tatatgttgt gggttctgat
      361 tttttgatca tgattattct ggggacgatt ggaactttct tgtttttctt atcagtatca
      421 ggttttttat taaaagttgt tcaaatgcgc aaaaagactt attataatgg tctgaatatg
      481 tttgtattaa aacaagtaaa taataagatc aatacacatg ttatttctac aacagttatt
      541 tccttaatgt tattgttaac gatcgggata ttatcatcgt ctttagcgat ggcctcagca
      601 atgaacacta cttatgaggg aaatagccca agtgatgtta ctgtattttc tagtaatact
      661 gaaattattg aaagatttaa acaggaaccg gtgtatcaac aaatcatcaa agaggattat
      721 cagttcaatt atcttaattt acaaggatta aaacaaggta tttttattga ttcaaaagat
      781 aatgcttata ctaatataga agcagtaaaa aatcaaccga tgcgggtaat caaagaaagt
      841 gattttaata aaatcatgga attaaatggt aaagaagatc aaaaaataca attggctaat
      901 aatcaatatg aattagtggc aacttatcct gtagcacttg aatattataa taagtttcta
      961 gctggcgata gtacaatcaa aataggagat actacgcttc aaagtaatac ggatcaagca
     1021 gtagaattgg caattactaa cgatagcggc aatgaagggt ttgttgttgt aaatgaccag
     1081 attgcaaata aatatgtgga agaacaagaa agttatttaa cttatctagt ggcagactat
     1141 caaggtgata aggaagaatg tgaggaacaa ttccaagatt tgcttaaatc ttttaatgaa
     1201 cagttaagga ctgaaagcag tccaacagtt attagtttta cccggattgg attagggcaa
     1261 tcgggaattg gaacgagtgc tttgtttact tttgttggac tgtatttagg aattgtattt
     1321 gcacttgcaa gtggtactgt gttagcaatt gaacaattat cagaatcttc tgataataaa
     1381 gaacgttatc gtatcttagg acagttaggt gcaagtaaac caatggtaaa tagaagcctt
     1441 ttagttcaaa ttggaatcac atttatgttt ccattaatcg tagctttaat tcatagtttt
     1501 gttgcactaa aagaaataaa ttatattatt agtttgatgg tcagcattaa tatcgctgat
     1561 aacatcttgg ttacgacgat ctttattgta attgtatatg gaggttatta tttagcgact
     1621 tatttagcta gtaatcgaat cattaatgaa taaaaaaccc gttatgggtt ttttagttta
     1681 agagcgtatg cggaagttcg tagattatat cgtcatggct gtatgtacga atttgtttat
     1741 gaggtaaaat agactggcga tcttctaaca atccatcaaa tacaatcttg tgtcgatagg
     1801 ggagcagtgc taaattacag acctggggca aagttgaatt aggataaatt tctttaagat
     1861 gattaacaag cccataaata gcataaacac cagtttcatt actaacgagc gtatagtcat
     1921 caagatttgt taaaattaaa aagtcatcaa taaagccagc tttaaaatca ttaataatag
     1981 tcagttcata attggttagg tgctcaggat tttcactgat atagtggtcg attatatttg
     2041 gattatcaaa tagtaaggtt cgaatttgag aaaaatcatc ttgctcaata taatcaagat
     2101 ttttcattga aatatctgta attttaaaaa cttggttagt atactctaat aaacttaaat
     2161 acagctgata aaaggtagaa ctctcttgct cactgaaata agaatctttg ttgattgtca
     2221 aatagtaatc atacggggat aatccataaa aagcagcact atgaattaaa ggaactgcag
     2281 cacgtaaatt cttttcaaga tgttcaataa cttgattatt tgtaattttt aattcattaa
     2341 tcatataaat caaatcgtta aggtcaccat gaatttgaca aaaaagcata atactatctc
     2401 taaataattc tcttacaaaa agattatcaa tttgttcgac tgcttcatag aggttagtga
     2461 tggctggttc acttaagtca aagccatatt tagcgatcgt tcttaattgt ttggcggtat
     2521 aagccgcagg agtttttttt actaatgttt ggagctgttc aattttttgg ataatcggcc
     2581 gatatggctc ataagctaat aataattcag tttccccttg ataaataaag taatgctgaa
     2641 atagatagtg atttgtatct aaatgagtgt ttacttcatt acgtgaaagt gaaggatcat
     2701 aactatgata aatatttact aattcttgtg gttcaataat acctctaatg gctagaatac
     2761 caatcaataa ttcattgatt tgatctattt gaatgacctt gtcaatatcc gcttgttcta
     2821 cagcattaat aacatttatc tttatgtctt cagggatttc taaatagtta ttattattag
     2881 gatttttaat tagtaagcat ttgttgatca atgtattgat tgtaggaata tctttacgat
     2941 aacttttggt tttttcaaaa ttttgtaaga aaacgatttc gtcataacat aaaatatcta
     3001 aaacattttg aatactgcca ttgtagtatg ctacgatctc ttgataaatc tgttttg
//
LOCUS       DAOMTA010000317         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1316_length_3055_cov_2.770333, whole genome shotgun sequence.
ACCESSION   DAOMTA010000317 DAOMTA010000000
VERSION     DAOMTA010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_3055_cov_2.770333"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PLK68_08910"
     CDS             complement(<1..1431)
                     /locus_tag="PLK68_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM90563.1"
                     /translation="MKEFTKVSIHNHLGGRGADRTLNDSYDIQCNFNFASACSLVDNA
                     NDNQFTLLALTHSNRLPAESYLKLKKYAKSKNITILPGAEINLKNEEERYLHTVVVFD
                     DKNPIEEIEKRIDDYINENKHNHLEIDQFLDLVIEYKCIISAHGIKQKTKQRSASTNA
                     DTFSELINLSDAIPVVIEDNHKYHKKTLKEELKDQLTSNELDWVDKTAIISAADRTSF
                     NQIVSPTYIWGNPNFDDLYYACFMSETRIKRESDIINKVNYIDKIVVDAYQDAQVRPK
                     EIVCSHGLNTIIGPSGSGKTMLLDIIRRKLTGKGLENKSISKNSNYEEVYDLNHIHLY
                     DGKGVELTKDSGYSIVEGEILYNKVITAYQADKESLLNELHLVIDKTELDSVIMRFNL
                     GISQYVKNSIEINKNRTEINTLLKANSSIIEFLDANVKPNNDTLDYSKDDNIASKIKS
                     INEKIRDIASDITDLDQKFSEISKIAN"
     gene            complement(1446..>3055)
                     /gene="brxL"
                     /locus_tag="PLK68_08915"
     CDS             complement(1446..>3055)
                     /gene="brxL"
                     /locus_tag="PLK68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HRM90564.1"
                     /translation="DFNEEDKRSSPIKIRNLKPIQLPHVDINEYKENRALFTKDEWVD
                     LLLRSIGMEPDELNEREKWLLLLRMIPLVENNFNICELGPRSTGKSHLYKEISPNSIL
                     VSGGQTTVANLFYNMGRKTVGLVGLWDCVAFDEVAGIKFKDKDGIQIMKDYMASGSFA
                     RGKEEKAASASMAFVGNINQSVDVLLKTSSLFDPFPPEMGQDTAFLDRIHCYLPGWEV
                     PKFRPDHFTNDYGFITDYLSEVMRELRKDQQGDALDKYFRLGKNLNQRDTIAVRRIIS
                     GLVKLVYPDGNYDKQQLEEIVKIALEMRRRVKEQLKKIGGMEFYDVNFSYIDNETFEE
                     NYVSVPEQGSSTLIPEGMCNPGQVYTVSQSKSGMIGLFRLESQMLPGNGKFDRNGIGT
                     DRECREATQIAFNYLKANANRISQSISTTTKDYLIHYQDLQGIGMTKTLAMPTLIALC
                     SIALSKPTVSSLAVLGDISISGTVMKVDNLANSLQVCLDSGAKKVLLPITSAADLATV
                     PSELIGCFNLIFYSSAEDAVFKALGVE"
BASE COUNT          948 a          558 c          376 g         1173 t
ORIGIN      
        1 attagcaatt tttgaaattt cactaaactt ttggtctaaa tcagtaatgt cactagctat
       61 atcccttatc ttttcattaa ttgatttgat ttttgaagct atattgtcat cctttgaata
      121 atctaaagta tcgttatttg gttttacgtt agcatctaaa aattcgatta tacttgaatt
      181 tgcttttagt aaagtattta tttcagttct attcttatta atttcaatac tgttttttac
      241 gtactgagat attcctaaat taaacctcat tattactgaa tctaattcag ttttgtctat
      301 caccaaatgc aattcattta acaagctctc tttatcagct tgatatgcag taattacttt
      361 attatataag atttctccct caacgattga ataaccagaa tcctttgtta attcaacccc
      421 tttaccatca taaagatgaa tatggtttag gtcatatacc tcttcgtaat tactgttttt
      481 agaaattgat ttgttttcta aacctttccc tgttaatttt cttctgataa tatccagaag
      541 catagttttt ccactaccag acggtccaat aatcgtattt aacccatgtg aacaaactat
      601 ctcctttggt cttacttgag catcttgata tgcatctaca actatcttat ctatataatt
      661 aactttattt attatgtcag attctctttt aatacgtgtt tcactcataa agcacgcata
      721 ataaaggtca tcaaaattag gattacccca tatataagta ggtgaaacta tttgattaaa
      781 acttgttcta tcagcagcag atatgatagc tgttttatcc acccaatcta actcatttga
      841 agttaactga tctttaagtt cctccttcaa cgtcttttta tgatatttgt gattatcctc
      901 aattacaaca ggaattgcat cagataaatt tattaactca gaaaaagtat ctgcatttgt
      961 gctagctgat ctttgttttg ttttctgctt tatcccatgt gcagagatta tacatttata
     1021 ttcaattacc aaatccaaaa attgatcaat ctctaaatga ttgtgtttat tttcatttat
     1081 ataatcatca attctcttct ctatctcttc tataggattc ttatcatcaa acactactac
     1141 tgtatgcaaa tatctttctt cttcattttt taaatttatt tctgcaccag gaagtattgt
     1201 aatgttcttt gattttgcat atttttttag ttttaaataa gattctgcag gcaatctatt
     1261 tgaatgtgtc aatgctaata aagtaaactg attatcattt gcattatcta ctaaactaca
     1321 tgcagatgca aagttaaagt tgcattgtat atcatatgaa tcatttaaag tacgatccgc
     1381 tcctctacca cctaaatggt tatgaataga gacttttgtg aattccttca acttatctcc
     1441 cccccttatt caactcctag tgctttaaat acagcatctt ctgcactgct atagaaaatt
     1501 aagttgaaac aaccaattag ttctgaaggt actgttgcta aatccgctgc agatgtaata
     1561 ggtaataata cttttttagc gccgctatct aaacatactt gtaatgaatt agctaagtta
     1621 tctaccttca ttactgtacc actgatgcta atatcaccta acacagctaa tgaactaact
     1681 gttggcttag ataaagcaat tgaacataat gcaattaatg ttggcatagc taatgtttta
     1741 gtcataccaa tgccttgcaa atcttgataa tgaatcaagt aatctttagt agttgtacta
     1801 attgactgac ttattctatt agcattagct tttaagtaat tgaaagcaat ttgtgtagct
     1861 tctctacatt ctctatctgt accaatgccg ttacgatcaa atttaccatt acctggtaac
     1921 atttgacttt ctaatctaaa taaaccaatc attcctgatt tagattgaga tacagtatac
     1981 acctgtccag gattacacat gccttctgga atcaatgtac ttgatccttg ttcaggtacg
     2041 gaaacataat tttcttcaaa agtttcatta tctatgtatg agaaattaac gtcatagaat
     2101 tccattccgc caattttctt taactgttct tttacacgtc ttctcatttc taatgcaatc
     2161 ttaacaattt cttctaattg ttgtttatca taattgccat caggatatac tagtttaact
     2221 aatcctgaaa taatacgtct aactgcaata gtatcacgtt gatttaagtt tttacctaat
     2281 ctgaagtatt tatctaaagc atcaccctgt tgatcttttc ttagttctct cataacttca
     2341 gaaagataat cagtgataaa accataatca tttgtaaaat gatctggtct aaattttgga
     2401 acttcccatc caggtaaata acaatggata cgatctaaga aagctgtatc ttgccccatt
     2461 tcaggtggga atggatcaaa taaactagat gtctttaata gtacatctac actttgatta
     2521 atattaccaa caaaagccat agaagctgat gctgcttttt cttctttacc tcttgcaaaa
     2581 gatccagatg ccatgtaatc tttcatgatt tgaataccat ctttatcttt aaacttgata
     2641 cctgcaactt catcaaaagc cacgcaatcc cataaaccaa ctaatccaac tgtttttctt
     2701 cccatattat agaaaagatt agctactgta gtttgaccac ctgatactaa aatactatta
     2761 ggtgagattt ctttatataa atgggattta cctgtacttc ttggtcctaa ttcacagata
     2821 ttgaagttat tttctactaa tggaatcatt ctaagtaata ataaccattt ttctctttca
     2881 tttaattcat ctggttccat accaatagaa cgtaataata aatcaaccca ttcatctttt
     2941 gtaaataatg ctctgttttc tttatattca ttgatatcta catgaggtaa ctgaattggt
     3001 tttaagtttc tgattttgat tggactagaa cgcttatctt cttcattgaa gtcat
//
LOCUS       DAOMTA010000318         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1317_length_3055_cov_2.230667, whole genome shotgun sequence.
ACCESSION   DAOMTA010000318 DAOMTA010000000
VERSION     DAOMTA010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1317_length_3055_cov_2.230667"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1939
                     /locus_tag="PLK68_08920"
     CDS             164..1939
                     /locus_tag="PLK68_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HRM90565.1"
                     /translation="MNEIQYLKEFTRLYKENSHDKYNREVECHFFMHKENRTKITSDD
                     YFLSCFPSAICGFGSRNTNFIYNCKLERLAKLIEGTHDVEKEELEELYTFWADENDKE
                     RLRRYYPNDIKELMPYDDFENDYYTAYPLYRLGGAYLDFEKLFDLGIDGLIHEIDSQP
                     LNSFLRACKKSLIYLKELIKLYRDDAMDINPELAYTLNELLEHRPQNMKEAIQLMWIY
                     VGVSEIRNYGRMDNQLARFLDDEQDAYKNIAEYFKVIRQRNTIYNGRIILGGEGRHDL
                     EKANKICSIALKVMKDLHLTEPQLTLRWSKDMPDSIFDNAIDCIESGCSYPLLYNDTV
                     NIKNVKESMNVSYKEAVDYVPLGCGEYVLDHKSIGSPNGIINLAKVLEGLVNDGKCMI
                     TNKKIVKHYLTKEPNTFEELYDVYKQELDYQIDLLAKQEKFEYDYMNNHCGYLFNSIL
                     YDNCIDRGKSLLNGGIDYLGGTLETYGNVNSGDSLYAIKKLVFDDKEIRYKDMVDAIR
                     NNFIGYEEIREKCLNVIKYGNDNPEVDEMINELNQYVAVTTKSKSEKYGLSSYLIVII
                     IKYPFTRGCFTNGFNIRIIRVVLTK"
     assembly_gap    2768..2798
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1191 a          320 c          498 g         1015 t
ORIGIN      
        1 attattttta ttatattata attttgattt aaataaaatt aaaaataaag ttagaaaaat
       61 aagataaaat gaatgctttg tttaataaag tttaaaaagc gaatatatgt taagtatgat
      121 ataccttgtt atgatactct ttatatgaaa aggaggtaga caaatgaatg aaattcaata
      181 tttaaaagag tttacgagat tgtataaaga aaatagtcat gataaataca atcgggaagt
      241 agaatgccat ttctttatgc acaaggaaaa tcgtacaaaa ataacgagcg atgattattt
      301 cttatcatgt tttcctagtg caatttgtgg ttttggatca agaaatacta actttattta
      361 caattgcaaa ttagaacgat tagcaaaatt aatcgaaggt acacatgatg ttgaaaaaga
      421 agaattagaa gaattgtata cgttttgggc tgatgagaat gataaagaac gtcttagaag
      481 atattatcca aatgatataa aagaattaat gccatatgat gattttgaaa atgattatta
      541 tacagcgtat cctttatata gattaggagg agcttatctt gattttgaaa aattatttga
      601 tttaggaata gatggattga ttcatgaaat tgattcccaa ccactcaata gttttttaag
      661 agcttgtaag aaatcgctta tttacttaaa agaattaata aaactgtatc gtgatgatgc
      721 catggacatt aatcctgaat tggcttatac gttaaatgaa ttacttgaac atagaccaca
      781 aaatatgaaa gaagcgattc aattaatgtg gatttatgta ggcgttagtg aaattagaaa
      841 ctatggaaga atggataatc aattagcacg ttttttagat gatgaacaag atgcatataa
      901 gaatattgca gaatatttca aggtaattag acaacgaaat acgatttaca atggtcgtat
      961 tattttaggt ggagagggtc gtcatgatct tgaaaaagca aacaagatat gcagtatagc
     1021 tcttaaagtt atgaaagatt tacatttaac tgaaccacaa ttaacactta ggtggtcaaa
     1081 agatatgcct gatagtattt ttgataatgc gattgattgt atagaaagtg gttgtagtta
     1141 tccattatta tacaatgaca cggttaatat taaaaatgtc aaagaatcaa tgaatgtatc
     1201 atataaagaa gcggttgatt atgttcctct tggttgtggt gaatatgttt tggatcataa
     1261 aagcattggt tcacctaatg gaatcattaa tttagctaag gtattagagg ggttggttaa
     1321 tgatggaaaa tgtatgatta ctaataaaaa aatagtaaag cattatttaa ctaaagaacc
     1381 taatacattt gaagaattat atgatgtata taaacaagag ttagattatc aaattgattt
     1441 gttagctaaa caagagaaat ttgaatatga ttatatgaat aatcattgtg gatacttatt
     1501 taattctatt ctttatgata actgtattga tagaggtaaa agcttattaa atggtgggat
     1561 tgactatcta ggtggaactt tggaaaccta tggtaatgtg aatagcggag atagtttata
     1621 tgccataaaa aaattagtct ttgatgataa agaaattcgt tataaagata tggttgatgc
     1681 aattagaaat aactttattg gatatgaaga gattagagaa aaatgcctta atgttatcaa
     1741 gtatggtaat gataatcctg aagtagatga aatgattaat gaattaaatc agtatgttgc
     1801 agtcacaaca aaatctaaat cagaaaaata tggattatct tcatatttga tagttattat
     1861 aattaaatat ccatttacac gaggatgttt taccaatggc ttcaacatta ggataattag
     1921 ggtcgtactt actaaataaa ataaagagtc ttaaagtata agattatgat agtttcatca
     1981 gtagttttga actgtaagag aagattaaaa tctatactta cctaacacca gtgatagttt
     2041 tcttttgctt tgtatcaaat attgccttga atttataaat attcataata tgtaaccact
     2101 ctagatatag tatagttgtc tagtctgata tagttaaacc gttatgctga tttgttaaca
     2161 tttttagaca aagaaaaaga actaagtatg aactgaattt caaaagttgg acagtttata
     2221 aatagttctt tttatgatta atctgttgtc caatagttat tacttcaaaa attccaatga
     2281 gaaagaagtt tatttcatga atcttctaac gattttacat catttttatt attttgctta
     2341 ttaaattctc acatcattat gataaaataa tcctctataa aatgccttaa tcttctaaaa
     2401 aatggaacat tatgttaatt ttattaataa taaaatatcg ttacaaaagt attagtattt
     2461 atctatctgg atttttgtga ttgaaaaaaa gtagatatca tttcctgata catatataat
     2521 actatttgtg aaggtttgac tgtatttttt tgtattttac agtaaaaaag tactaagaaa
     2581 taatgcttag atttgaaacg tttgaaagaa aagttttaaa aaaatcaaaa gaagaaatca
     2641 atgaaaagtc aaacgtacat atgagccata aaaatgaaaa ccagtcattc aataactttg
     2701 attttattta cgtttaattg gtagtcaaaa gctgatgtca tagatgttaa aacagaagta
     2761 catcaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat taaaagacaa aatgaagatc
     2821 agtactgaaa agaacctcaa agaatatgaa acgcttctac aagctcttaa atgctcaaac
     2881 acgctccaaa caaagaacaa cgaaacaagg actgtgagta cgcaataaaa aaagaacaga
     2941 tactaagcaa tctaaagcat tacaaaaaag tccgtaatgt attttacgaa cttttttgta
     3001 ccaaatgttc ttgaaccgct gaactattag aagtgaaaaa agataagtag tcgag
//
LOCUS       DAOMTA010000319         3042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1328_length_3042_cov_2.545698, whole genome shotgun sequence.
ACCESSION   DAOMTA010000319 DAOMTA010000000
VERSION     DAOMTA010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1328_length_3042_cov_2.545698"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..231
                     /gene="rpsR"
                     /locus_tag="PLK68_08925"
     CDS             1..231
                     /gene="rpsR"
                     /locus_tag="PLK68_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRM90566.1"
                     /translation="MAFRKQRMGRKKVCYFTKNKITYIDYKDVELLKRFVSANGKIIP
                     RRVTGTSAKYQRMLATAIKRARQMALLPYVGE"
     gene            complement(329..1540)
                     /locus_tag="PLK68_08930"
     CDS             complement(329..1540)
                     /locus_tag="PLK68_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM90567.1"
                     /translation="MPIRERKNKSGIVFDITVNDGYKLDVHGNKVQNRKYKTFKPPKD
                     MSIRKARSIAREMELEMLRQFERKENLGSEKQLIDVWHWYKNFYAPNRLRESTIDLIT
                     QIMENKVIPDLGFIKIGDFTTARITQFLNEVAVIKDKETGRPLRPKQYYKDSYVRAIH
                     STLSRIFQVAISQGWIKENPCENAIRPKKNKTEKKPPLELHQIKDIIKKTTEFTVLNA
                     VLQFQIYTGMRIGETLALTWDDVDFKKRTIKVNKTVNIISGNIYVGPPKTDNGFRTLY
                     ISDTLMNLLKLVKEDQDEKKKALCNVYEDQNLVFAGNTGNYINRHTVSSRLASIKKGT
                     DYEYITVHFLRHTNATLLLMNDVDLKTVSAHLGHSDINITADTYTDVLHARQKFVANL
                     VDLNLEDENDE"
     gene            complement(1515..1895)
                     /locus_tag="PLK68_08935"
     CDS             complement(1515..1895)
                     /locus_tag="PLK68_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90568.1"
                     /translation="MKRKVMPNRLKYARINAGYSQKDVCDISGFISRNSLGNCETGYE
                     YPGNKVLHFLPGLYKVSLDYLFCEDCYPTHDDFVKEVLFLDDKSIEILKTLRNQKVKL
                     DDFKDFLLNLIGEDKDYAYKRKKK"
     gene            complement(1892..2251)
                     /locus_tag="PLK68_08940"
     CDS             complement(1892..2251)
                     /locus_tag="PLK68_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90569.1"
                     /translation="MATYYRDKRLPNRFLKCRLDKNLTQEQLAYYLGLKRRAILNYEK
                     ANTYPKMETLIEMHRYLDVSLDYLLVLDPYKNHVEYTKNVLGLTEYFLQLLESIQNEK
                     IYRKINDYIHSQYGEQL"
     gene            2511..>3042
                     /locus_tag="PLK68_08945"
     CDS             2511..>3042
                     /locus_tag="PLK68_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM90570.1"
                     /translation="MEYVFKVVDLSQLDEFPNHPYQVRIDEDLRNLQNSISERGVDEP
                     LIVRNINDRYQVISGHRRMKACELAGIHDVPIRVVDLDDDEAVIMMVDSNLHRRKLLK
                     SELAFAYRMKHEALKHQGKIVEDLTSSQLETKYSTDTARQLAAEKGTSRAQVYRYIRL
                     TYLNKGLLDLVDEERIA"
BASE COUNT          955 a          471 c          525 g         1091 t
ORIGIN      
        1 atggcattta gaaaacaaag aatgggtaga aaaaaagttt gctacttcac taaaaacaaa
       61 attacttaca tcgattacaa agatgttgaa ttattaaaaa gattcgtttc agctaacggg
      121 aaaattatcc caagacgtgt aactggtact agcgctaaat atcaaagaat gttagctaca
      181 gctatcaaac gtgctcgtca aatggcttta ttaccatacg ttggtgaata gttaaaatga
      241 tgaaaaagcc tcgataaaat cgaggctttt tttgtggaat agcgttttat acgtgattta
      301 tacgtgattt tgaacggaga agtgcatatt attcgtcgtt ttcgtcctct aaattgaggt
      361 caactagatt ggcgacgaac ttctgacgag catgtaatac atctgtatat gtatcagcgg
      421 taatattaat atcactatga ccaagatgag cagatacagt ctttagatca acatcattca
      481 tcagtaatag tgttgcattc gtatgacgca agaagtgaac ggttatgtat tcatagtccg
      541 ttcctttttt tattgaagca agtctagatg atacagtatg acgattaatg taattaccag
      601 tattacctgc aaagactaga ttttgatctt catatacatt acataaagct ttcttctttt
      661 catcctgatc ttccttgacg agtttgagta agttcataag tgtatcagat atatataaag
      721 ttctaaaacc attatcggtt tttggtggac caacataaat attaccacta atgatgttga
      781 cagttttatt tactttgatt gttcttttct taaaatcaac atcatcccag gtaagtgcta
      841 aggtttcacc aattcgcatg cctgtataaa tctgaaattg aagaacagca ttgagtactg
      901 taaactctgt ggttttctta attatatcct ttatttgatg aagttccaat ggtggcttct
      961 tttctgtttt attcttttta ggacggattg cattttcaca tggattttct ttaatccatc
     1021 cttgagatat agctacctga aagatacgac taagtgtcga atgaattgca cgtacataac
     1081 tatctttata gtattgttta ggacgaagtg gtctgcctgt ttctttatct tttataacag
     1141 caacttcgtt aagaaattga gtaatacgtg ctgttgtaaa atcaccgatt tttatgaatc
     1201 ctagatcagg tatgactttg ttttccatga tctgagtaat cagatcgatt gtagattctc
     1261 ttaaacggtt aggggcataa aagtttttat accagtgcca tacatctata agttgctttt
     1321 cgctacctaa gttttctttt ctttcaaatt gtctaagcat ttctagttcc atttcacgag
     1381 caatgcttct ggcttttcga atactcatat ctttaggtgg tttaaatgtc ttatattttc
     1441 tgttttggac tttatttcca tgaacatcga gtttatagcc atcattaact gtaatatcaa
     1501 atacgatacc tgatttattt tttctttctc ttataggcat aatccttatc ctccccgatt
     1561 aagtttaata aaaaatcttt aaagtcatca agtttaactt tttgattacg taaggtttta
     1621 agtatttcga tgcttttatc atctagaaat agtacttctt ttacaaaatc atcatgtgta
     1681 gggtaacaat cttcgcaaaa tagataatct agtgaaactt tgtataaacc aggtaaaaag
     1741 tgcagtactt tatttcctgg atattcatat cctgtttcac agttacctaa tgaatttctt
     1801 gaaataaaac cagatatatc acaaacatct ttttgggagt atccggcatt tatccttgca
     1861 tattttaaac gattaggcat aacttttctt ttcataactg ttctccatat tgtgagtgaa
     1921 tgtaatcatt gattttacga taaatctttt cattttggat gctttctaga agctgcaaga
     1981 agtattcggt taatcctaga acatttttgg tatactcaac gtgattttta taaggatcaa
     2041 gtactaataa atagtctaat gaaacatcta gataacgatg catttcaatt aatgtttcca
     2101 tctttggata agtatttgct ttttcataat taagaatagc tctacgcttt aatcctagat
     2161 agtaagccaa ttgttcttgt gttaagtttt tatccaatct gcatttcaag aaacgattgg
     2221 gtaaacgctt atctcgataa tatgttgcca ttgtgattcc tccttgtttc aaacaggtgg
     2281 cgtgcctgtt atatggcaat cttacaaatt aaaattttta aaatcaaagg tgcaattttt
     2341 actgcaattt tccttctttg ttatatagtt catgtttatt cgtgaaatta gttgagagat
     2401 ttaactggta ataaaatatc agttatcgtc tctaaaatta aatatgaaaa ttttttgagc
     2461 gaatgcctaa tttatgggtg ttcgctcttt tttttaggag agtgaactat atggagtatg
     2521 tattcaaagt tgtggatcta tcccaacttg atgaatttcc gaaccatcca tatcaggtga
     2581 ggatagatga agatttaaga aatcttcaaa acagtattag tgaaagaggg gtagatgaac
     2641 cattgatagt aagaaatatc aatgatcgct atcaagttat ctcagggcat cgaagaatga
     2701 aagcatgtga acttgctggt attcatgatg tacctattag agttgttgat ctagatgatg
     2761 atgaagcagt tatcatgatg gtcgatagca atttacacag aagaaaatta ttaaagagtg
     2821 agttagcatt tgcctatcga atgaagcacg aagccctaaa acatcagggg aagatagttg
     2881 aggatttaac ttcgtctcaa cttgagacga agtatagtac tgatactgca agacaattag
     2941 cagctgaaaa aggaacaagt agagcgcaag tttatcgcta tattcgatta acttatctca
     3001 ataaagggtt attggatctt gttgatgaag aacgtatcgc aa
//
LOCUS       DAOMTA010000320         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1334_length_3032_cov_2.129997, whole genome shotgun sequence.
ACCESSION   DAOMTA010000320 DAOMTA010000000
VERSION     DAOMTA010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1334_length_3032_cov_2.129997"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1031)
                     /gene="pilM"
                     /locus_tag="PLK68_08950"
     CDS             complement(135..1031)
                     /gene="pilM"
                     /locus_tag="PLK68_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRM90571.1"
                     /translation="MKNIIYLSNTSAQLVSGVCNGSNQIDISDFQDYQLPEGTMLDGA
                     IMDEDALKDVLRIINKKGIGDCKLVIDSGQIVHKNLIVPKIKDKEIHAIVKEEIDSLE
                     NGEHDLIYDYTILKDKLVDKPGIEILCCAMKKQMIEDYLNIFDDVGIEITAIDISLNA
                     IDKLIEDIIRLSQRNFVIAVINGNDIALYLFEEGKYVFSNRSRLFSERGSSSFTMEVS
                     NILIKFKQFIKTADYNQNIERVYFCGLDDYEEKMLFEVVSDSVDIRAMRLANSNTIYY
                     SGNSKEFLLHKYVYAIGSLCER"
     gene            complement(1041..2234)
                     /locus_tag="PLK68_08955"
     CDS             complement(1041..2234)
                     /locus_tag="PLK68_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM90572.1"
                     /translation="MAIYKYVARDINAKKITGKTEARDEHELSQLLRVKDMYLISHKD
                     ITKEETKNYKMKLKELANFCREIGTMMNSGLPLIRTVSILASREDNKKLKAIYNDIYV
                     KLQQGQTLSDALKEQGKAFPDILIQMVRSGEASGNMQDTMMVLNNQFTNDNKIKNKVK
                     SAMTYPVILGIVTIVVLLIVYTAVLPSFFSMFEGMELPLITEINIIISKFIMSYWYAL
                     LIGILVLILIIVSLLQLPKVKYQFDRFKLKMPIIGKLMKIIYTSRFARTLCSLYSSGI
                     SIVNAMVIVKSTIGNKYIETQFDNSIKAVRNGEALSVAIGMIDGFDIKLTSSVYIGEE
                     SGNLEDLLSSLADDFDYEAMLASEKMVAILEPAMIIVLAVVICVIIISVLVPIYSMYQ
                     NVGSM"
     gene            complement(2248..3012)
                     /locus_tag="PLK68_08960"
     CDS             complement(2248..3012)
                     /locus_tag="PLK68_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRM90573.1"
                     /translation="MDFLIIAYIYMFILGSCIASFINVLIYRIPRDLDFVNGRSFCPS
                     CHNTLKPYEMIPVFSWLFLKGKCRFCKEPISPRYPLIELCGGLLAMLCFYRYGFTWMT
                     LVSFVLAMILLTITMIDFDTMTIPNGLVIALVAPVIVCAVLEPQISISSRVIGMASVS
                     GFMLLMTLAIPDCFGGGDMKLMFVCGFMLGWINTLLAGFIGLLAGGIYASYLMVTKKS
                     KEQSHMAFGPYLCLGIFIALLYGNEIIRVYLSFFNL"
BASE COUNT         1074 a          612 c          319 g         1027 t
ORIGIN      
        1 aatgaatact aaaaccaaag gcaagataag atatttaagg tattcttgga aaatattttt
       61 tttcttggta gctagataat attgttccag tagatcaata tctttgcttt tatttcgctt
      121 gaacattatt atccctacct ttcacataaa cttccaatag cataaacata cttatggagt
      181 aagaattcct tagagttacc actgtaataa atcgtattac tattagctaa gcgcattgcc
      241 cttatatcga cactatcact tactacttca aatagcattt tttcttcata atcatccaat
      301 ccacaaaaat atactctttc aatattttga ttataatcag ccgttttaat aaactgctta
      361 aacttaatta aaatattgct tacttccata gtaaaggaac tgcttcctcg ctcagaaaac
      421 aatcttgaac ggttcgagaa aacatactta ccttcctcaa ataaatataa agctatatca
      481 ttaccattaa tcaccgcaat aacaaagttt ctttgagaaa gtctgataat atcttctatt
      541 aacttatcga ttgcattaag agaaatatca attgcagtta tttcaatccc cacatcatca
      601 aaaatattta aataatcttc tatcatctgc tttttcattg cacaacaaag aatctcaatc
      661 cctggtttat ctactaattt atcttttaaa attgtataat catagatcaa atcatgttca
      721 ccattttcta agctatcaat ttcttcttta acgattgcat gtatttcttt atccttgatc
      781 ttaggaacaa ttaaattctt atgcacgatt tgtccactat caataaccag cttacaatca
      841 ccaattcctt ttttattaat tatgcgcaat acatctttta aagcatcttc atccatgatt
      901 gcaccatcaa gcatagtccc ctcaggtaat tgataatctt gaaaatcact aatatcaatt
      961 tgattactgc cattacatac accactgacc aattgtgcac tcgtattcga taaatatatt
     1021 atatttttca taaagtcccc ctacatactt ccaacatttt gatacattga ataaattggt
     1081 actaacacag aaataataat tacacaaatt actactgcta aaacaataat catagctggt
     1141 tccaaaatag caaccatttt ttctgacgct aacatagctt cataatcaaa atcatcagct
     1201 agtgaactca gtaagtcttc aaggtttcca ctttcttcac caatatatac acttgaagtt
     1261 aatttaatat caaatccatc aatcattcca attgcaactg acaatgcctc accatttcta
     1321 accgctttaa tagagttatc aaactgcgtt tcaatatatt tattaccaat cgtagatttt
     1381 acgatcacca ttgcattaac aattgaaatc ccactagaat ataaagagca taaagttctt
     1441 gcaaatcttg aggtataaat tattttcatt agtttcccaa ttatcggcat ttttaattta
     1501 aagcgatcaa actgatattt aacttttggt aattgcaaca atgacacaat tattaatatt
     1561 aatactagga tgccaatcaa cagtgcgtac cagtaactca taataaattt actaattata
     1621 atattgattt ctgttattaa tggtaattcc attccctcaa acatactaaa gaacgatggt
     1681 aacacagctg tataaacaat caataaaaca acaattgtaa ctattcctaa tatgactgga
     1741 taagtcattg ctgatttaac tttattttta atcttattat catttgtaaa ttgattattt
     1801 aagaccatca ttgtatcttg catgttcccg ctcgcttcac cagatcttac catttgaatt
     1861 aatatatcag gaaaggcttt cccctgttct tttaatgcat ctgataatgt ttgtccctgc
     1921 tgcaatttta catatatatc attataaatc gcttttagtt tcttattatc ttcacggctt
     1981 gctaaaattg aaaccgttcg aatcagcggt aatccagaat tcatcattgt tccaatctca
     2041 cgacaaaaat tagctaattc cttcaatttc atcttataat ttttagtttc ttctttagtt
     2101 atatctttat gagaaatcaa atacatatct ttaacacgta aaagctgact aagttcatgc
     2161 tcatccctag cctccgtttt tcctgttatt ttctttgcgt ttatatcacg tgctacatat
     2221 ttataaattg ccatttctct tcctcactta taaattaaaa aatgatagat acaccctaat
     2281 tatttcattt ccatataata gtgctataaa tatccctaaa cacaaatatg gtccaaaagc
     2341 catgtgtgac tgctcttttg acttttttgt taccatcaaa taactagcat aaatcccccc
     2401 agctaatagt ccaataaaac cagctaataa agtatttatc cagccaagca taaatccaca
     2461 aacaaacatc agtttcatat caccaccgcc aaaacagtct ggtatggcta atgtcattaa
     2521 taacataaac ccactaactg atgccattcc aataacacga ctactaatac taatttgagg
     2581 ctctaaaact gcacagacaa taactggagc aaccaaagca ataactaatc cattagggat
     2641 agtcatagta tcaaaatcaa tcattgtaat cgtcaataaa atcattgcta aaacaaaact
     2701 aactaatgtc atccaagtaa aaccataacg ataaaagcat aacattgcta acaagccacc
     2761 acataattca attaatggat accgtgggct aattggctct ttgcaaaagc gacatttacc
     2821 ttttagaaac agccaactaa aaacaggtat catctcatat ggttttaatg tattgtgaca
     2881 actagggcaa aaacttcgtc cattaacaaa atccaaatct cgaggaattc gataaattaa
     2941 tacattgata aaactggcaa tacagcttcc caaaataaac atatagatat aagctattat
     3001 caaaaaatcc acattttcac cccttatcaa ac
//
LOCUS       DAOMTA010000321         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1338_length_3028_cov_2.367978, whole genome shotgun sequence.
ACCESSION   DAOMTA010000321 DAOMTA010000000
VERSION     DAOMTA010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1338_length_3028_cov_2.367978"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLK68_08965"
     CDS             <1..313
                     /locus_tag="PLK68_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRM90574.1"
                     /translation="KTLKDVEVKWNDQHVACLVLSSGGYPGNYQKGIEIENIDDCDDC
                     VVFHAGTAIKDGKLVTNGGRVLNICATGVSLDEVREKVYAVAKKIDFEGKYYRSDIGL
                     R"
     gene            324..>835
                     /locus_tag="PLK68_08970"
     CDS             324..>835
                     /locus_tag="PLK68_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791641.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRM90575.1"
                     /translation="MSDVKRIYVEKRTAYATEANEIKHNLIEQLGLEIDTLRIINRYD
                     VQGVSDGILKQGINTILAEPMVDDVYQEEFPTNNQEVVFAIEFLPGQYDQRADSCEQC
                     FAILTGNSSAKVKCARVFAITFKGDKDEILTKIQSFLINPVDQRLASLEKPADLNDVA
                     PEIKPVPTING"
     assembly_gap    836..852
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <853..>3028
                     /locus_tag="PLK68_08975"
     CDS             <853..>3028
                     /locus_tag="PLK68_08975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRM90576.1"
                     /translation="TDNGMAMNYEDLKVTQDYFKNEEKRDPTEAELKVLDTYWSDHCR
                     HTTFATVITDLDIENGAFKEILEKDIEDYKTSRHLVYGIDTKRPLTLMDLATISMKEL
                     RKTGYLDDLEVSEEINACSVEITVHTTDGDEQWLLMFKNETHNHPTEIEPFGGAATCL
                     GGAIRDPLSGRAYVYQSMRITGAGDPRKSLAETMAGKLPQRKLCQESAHGFSSYGNQI
                     GLTTGYVHEIYDDGYVAKRMELGAVVAAAPKEQVKRLEPLKDHIVLLIGGRTGRDGIG
                     GATGSSKSHDVKSIETAGAEVQKGNPVEERKIQRLFRNKEVSEKVVRCNDFGAGGVCV
                     AVGELAPGLVIDLDAVLKKYEGLTGTELAISESQERMAIVIDEKDADFIKAECAKENL
                     EVVQVAVVTDQNRLVMKHMGEDIVNISRDFLDAAGAKRYQNVKITLPDFEKTPFDKQS
                     QTSFVETVKETLSKLSVASQKGLIERFDSSIGNGTVLSPFGGIKYHTETEGMAALIPV
                     LGKETTTASVMTYGYNPLISKWSPYHGAMYAIVESVAKIVAMGGNYHTIRFSFQEYFE
                     KLLDNPITWGKPTAALLGAYRVQSALKLASIGGKDSMSGSFEDLHVPPTLVSFAITSG
                     DVNDIMTPEIKETGHVLAEIIINKDQYHIFDFNHLQKQYDAIMELMKDKKIYSAYTVK
                     DGGVIEAVSKMAFGNGVGVAFNNENSLESYIVRDYGNIIIEV"
BASE COUNT         1047 a          389 c          643 g          932 t
ORIGIN      
        1 aaaaacttta aaagatgttg aagttaaatg gaatgatcaa cacgttgctt gtttagttct
       61 ttcaagtgga ggatatccag gtaattatca aaaaggaatt gaaatagaga atattgatga
      121 ctgtgatgac tgtgttgttt tccatgcggg gacagcaatc aaagatggaa aattagttac
      181 taatggtggt cgggtcttaa atatttgtgc tacaggagtt tcattagatg aggttcgtga
      241 aaaagtttat gcggtagcaa aaaagattga ttttgaaggc aaatattacc gaagtgatat
      301 cggacttaga tagggggatg aaaatgagtg atgtaaagcg tatttacgtt gaaaaaagaa
      361 cagcgtatgc aacagaagca aacgaaatta aacataattt aattgaacaa ttagggttag
      421 aaattgatac cttaagaatt atcaaccgtt atgatgttca aggagttagt gatggcattt
      481 taaaacaagg aatcaacact atcttggctg aaccaatggt cgacgatgtt tatcaagaag
      541 aatttcctac taataatcaa gaagtagttt ttgcaatcga atttttacct ggtcaatatg
      601 atcaaagagc agattcatgt gaacaatgct ttgctatttt aacagggaat agtagtgcta
      661 aagttaaatg tgcacgggta tttgccatta cttttaaagg cgataaagat gaaattttga
      721 cgaagattca aagcttcttg attaatcctg ttgatcagcg tcttgctagt ttagaaaaac
      781 cagctgattt aaatgatgtt gctccagaaa ttaaaccggt gcctacaatt aatggnnnnn
      841 nnnnnnnnnn nntaactgac aatggtatgg ctatgaacta tgaagattta aaagtaactc
      901 aagattattt taaaaatgaa gaaaaaagag atcctactga agctgaatta aaagtattag
      961 atacttattg gtctgatcac tgccggcata caacatttgc aacagttatt acagatttag
     1021 atattgaaaa tggagctttt aaagagatct tagaaaagga tattgaggat tataaaacta
     1081 gtcgtcattt agtttatggt attgatacaa aacgaccttt aacattgatg gatttagcca
     1141 caatctcaat gaaagagtta cgtaaaactg gttatttaga tgatttggaa gtatctgaag
     1201 aaattaatgc ctgctcagtt gagattacgg tacatacaac tgatggtgat gagcaatggt
     1261 tattaatgtt taaaaatgaa acacataacc atccaactga aattgagcca tttggtggag
     1321 ctgctacttg tttaggtgga gcaatccggg atccgttatc tggtcgcgct tatgtatatc
     1381 aaagtatgcg gattacaggg gctggagatc ctcgtaaatc attagcagaa acaatggctg
     1441 gtaagttacc tcaacgtaaa ttgtgtcaag aatctgccca tggtttttct tcttatggta
     1501 accaaattgg gttaactaca ggttatgttc atgaaattta tgatgacgga tatgtagcta
     1561 aaagaatgga attaggagca gttgttgcag cggcaccaaa agaacaagta aaacgtctag
     1621 aacctttaaa agatcatata gttttattga ttggtggtcg tactggacgt gatggtattg
     1681 gaggagcgac aggttcttct aaatctcacg atgttaaatc aattgaaaca gctggggctg
     1741 aagttcaaaa aggaaacccg gttgaagaaa gaaaaattca acgtttattt agaaataaag
     1801 aagttagtga aaaagtggtt cgttgtaatg actttggagc tggtggtgtt tgtgttgccg
     1861 ttggtgaatt agctccagga ttagtaatcg atttagatgc agtcttgaaa aaatatgaag
     1921 ggttaacagg aacagagtta gctatttctg aatcacaaga acgaatggct attgtaattg
     1981 atgaaaaaga tgcggatttc attaaagctg agtgtgctaa agaaaaccta gaagtagttc
     2041 aagttgcagt ggttactgat caaaacagat tagtaatgaa acatatgggt gaagatattg
     2101 ttaatatttc tcgtgatttt cttgatgctg caggcgctaa acgttatcaa aatgttaaga
     2161 tcactttacc agattttgaa aagacacctt ttgataaaca gtcacaaact agttttgttg
     2221 aaactgtgaa agaaacttta tcaaaattaa gtgttgcttc acaaaaaggg ttaattgaaa
     2281 gatttgactc ttcaattggt aatggaacag tattatcacc atttggtgga attaaatatc
     2341 atactgaaac tgaaggaatg gcagcattga ttccagtgtt aggaaaagag acaacaacag
     2401 cttcagttat gacttatggt tataatccat tgatttcaaa atggtcacca tatcacggag
     2461 caatgtatgc aattgttgaa tcggtagcga agattgtggc tatgggtgga aattatcata
     2521 caattagatt ttcattccaa gaatactttg agaaacttct agacaaccca attacttggg
     2581 gaaaaccaac ggctgcttta ttaggggcat accgtgttca aagtgcgtta aaattagctt
     2641 caattggtgg aaaagacagt atgtcaggat cttttgaaga tttacatgta ccaccaactc
     2701 tagtttcttt tgctattaca agtggagatg ttaatgatat catgacacct gaaattaaag
     2761 aaactggtca tgttttagct gaaattatta ttaataaaga tcaatatcat atatttgatt
     2821 ttaatcatct tcaaaaacaa tacgatgcga ttatggaatt aatgaaagat aagaaaatct
     2881 atagtgctta tactgttaaa gatggtggtg ttattgaagc cgttagtaaa atggcttttg
     2941 gtaatggtgt tggagtagct tttaataatg aaaatagtct tgaaagctac atagtgcgtg
     3001 attatggtaa tattattatt gaggttaa
//
LOCUS       DAOMTA010000322         3024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1341_length_3024_cov_2.835635, whole genome shotgun sequence.
ACCESSION   DAOMTA010000322 DAOMTA010000000
VERSION     DAOMTA010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_3024_cov_2.835635"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLK68_08980"
     CDS             complement(<1..1059)
                     /locus_tag="PLK68_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90577.1"
                     /translation="MSMTDRLTEGLLKVASKISNQRHMTAIKNAFTALLPIIITGAFC
                     TLFSNVVCSTTTTGISLAKIDGFAWLEELTPLFTAANYATLNFFTIGTVVLIAIEMAK
                     QLGHKETVAPVVAISAYVSLCDSFTKVAVEGVTDLVEVVNVLPREFTNAQGLFLGMIV
                     AIVSMEIYCRLADSGKLAIKMPESVPSNVSSAFNALFPAVLTILIISAFGLLFTKVTG
                     NSIYNMISTWIQAPLRGILTGLPGYLLIFFLSTCFWVIGIHGTQVLKPVYQATMLEAV
                     IANTDAAANGQTPQFILNETFISCFTTMGGAGCTIGLLFAMLLASKRSDHRTIAKLSL
                     APGLFNINETMTFGLPIVL"
     gene            complement(1466..2944)
                     /locus_tag="PLK68_08985"
     CDS             complement(1466..2944)
                     /locus_tag="PLK68_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM90578.1"
                     /translation="MTKQFPEGFLWGGATAANQFEGGWKEGGKGISVSDVALFTDPKS
                     LKELTDVHGLCDITDEMIDQALATDDEVYYPKRHASDFYHHWKEDIKLLGEMGFKVYR
                     LSIAWSRIFPNGDELVPNEAGLKFYDDIFDECAKYGIEPLVTMSHYEPPLEFARKYNG
                     WYDRRAIDFFVRYVDVITKRYKNKVKYWLTFNEIDSIIRHPFMTGGLIESRFKPEEFE
                     EVCFQAMHHQFVASALATKVTHDNIPDAKVGCMLTKLTYYPYTCKPEDVLEAQQRMRS
                     IYCFSDTQVHGEYPAYLLSMYKNKGFNINMTEEDLRIMKEYPVDFISFSYYSSSCVAK
                     DDTGLNKTAGNTVTAIKNPHIPSSDWGWQIDPIGLRVSLVDLYDRYRKPLFIVENGLG
                     AKDELIDGKVHDDYRIDYLKQHCQAMYEAIHEDGVELMGYTTWGCIDLVSNSTNQMSK
                     RYGFVYVDVDDYGNGSYKRYKKDSFDWYKQVIATNGASILED"
BASE COUNT         1018 a          594 c          392 g         1020 t
ORIGIN      
        1 taatacaatt ggcaatccaa aagtcattgt ttcattaata ttaaatagcc caggggctaa
       61 agatagttta gcaattgttc gatggtcgga acgttttgag gccagcagca tagcaaataa
      121 tagtccaatc gtacagcctg ctccacccat tgttgtaaaa caactaataa atgtttcatt
      181 taaaataaat tgtggggttt gaccattagc tgcagcatca gtatttgcga taacagcttc
      241 taacatggtt gcttgataaa ccggttttaa tacttgagta ccatggatac caataaccca
      301 gaaacaagtt gaaaggaaga atattaataa atatcccggt aatcctgtca aaatacctct
      361 taacggcgct tgaatccatg ttgaaatcat attgtaaatt gaattccctg ttacttttgt
      421 aaataaaagt ccaaaggccg aaataatcaa aattgtaagt actgccggaa ataatgcatt
      481 aaatgctgat gaaacatttg atggcactga ttcgggcatt ttaattgcta attttccact
      541 atctgctaag cgacaatata tttccattga aacaatagct acgatcattc ctaaaaataa
      601 tccttgtgca ttagtaaact cacgcggcaa tacattgact acttcaacta aatcagttac
      661 tccctcaact gcaactttag taaacgaatc gcacaaagat acatatgcag aaattgcaac
      721 aactggtgcc actgtctctt tatgtcctaa ttgtttagcc atttcaattg ctattaacac
      781 tactgtacca atcgtaaaga aatttaaagt agcgtaatta gctgcggtaa ataatggtgt
      841 caattcttca agccaagcaa atccatcaat tttagctaag ctgattcctg tagtagttgt
      901 actacacaca acattactaa acagcgtaca aaatgctccc gtaataataa ttggcagcaa
      961 agcagtaaat gcatttttaa tcgctgtcat atgacgttga ttagaaattt ttgaagcgac
     1021 ctttaatagc ccctcggtaa gacgatcagt catactcata actctttctc cttttatcaa
     1081 ttaaaatttt attgtgcaca aaatttaatt tatagaatga ttgtaaccgc taacaaattt
     1141 tagttctacc gtagaaaatt gctttggttg aaaaacgtca agatattcaa tatttagttt
     1201 tactttttga aaatttcgtt caaaatttaa taaataaaaa ctattaaaac tttctctcta
     1261 acagccaact caatctaaat ttatttacta tccttcacaa ttacctgttt taaaatatct
     1321 tattctaagc cgattacttt tcctaatttg agtccaatcc tcataccttc agttatacct
     1381 ctttgaattc attatcatga tgcttatctt taattgtaat cattttttat aaacaacttg
     1441 aatcataaaa taataaaaag aggacttagt cctctagtat agatgcccca tttgtcgcaa
     1501 taacttgttt ataccagtca aacgaatctt ttttataacg tttatatgaa ccattaccat
     1561 aatcatccac atcaacatac acaaaaccat aacgtttaga catttggttt gttgaattag
     1621 ataccaaatc aatacatccc catgtggtat aacccattag ctcaacacca tcttcatgaa
     1681 tggcttcata cattgcttga caatgttgtt ttaaataatc aattcgataa tcatcatgta
     1741 ccttgccatc aatcaattca tctttagccc ctaagccatt ttctacaata aataacggtt
     1801 tacgataacg atcgtataga tcaactaatg atactcttaa accaattgga tcaatttgcc
     1861 aaccccaatc agaactagga atatgaggat ttttaattgc agttacagta ttacctgcag
     1921 ttttatttaa tccagtatca tctttagcca cacagctaga tgaataataa ctaaatgaaa
     1981 taaaatcaac tggatattct ttcataattc ttaaatcttc ttctgtcata ttaatattga
     2041 agcccttatt tttatacata cttaataaat atgctggata ctcaccatga acttgggtat
     2101 cagagaaaca gtaaattgag cgcattcttt gttgtgcttc taaaacatct tcaggtttgc
     2161 atgtatatgg ataatacgtt aatttagtca acatacaccc cacttttgca tcaggaatat
     2221 tatcatgagt gaccttagtc gctaaagcac tagcaacaaa ttgatggtgc atagcctgaa
     2281 aacatacttc ttcaaattct tccggtttaa atcttgattc aatcaaacca ccagtcataa
     2341 atggatgtct aataattgaa tctatctcat taaatgtaag ccaatattta actttatttt
     2401 tataacgttt agttattaca tcaacatatc taacaaaaaa atctattgct cgacgatcat
     2461 accagccatt atactttctt gcaaactcta gtggtggttc atagtgtgac attgttacta
     2521 atggttcaat tccatacttt gcacactcgt caaatatgtc atcatagaat tttaatcccg
     2581 cttcattagg tactaattca tcaccattgg gaaatatccg actccaggca attgacaaac
     2641 gataaacctt aaatcccatt tcccctaata atttaatatc ttctttccag tgatgataaa
     2701 aatcactagc atgtcgtttt ggataataaa cttcatcatc cgttgctaat gcctgatcaa
     2761 tcatttcatc agtaatatca cataatccat gtacatcagt taactctttt aaacttttag
     2821 gatccgtaaa taatgccacg tcacttaccg aaataccttt tccaccttct ttccaaccac
     2881 cttcaaattg attagcagca gtggcaccac cccataaaaa tccttcagga aattgttttg
     2941 tcattttatc gacctccgta ttctaattta ttttaatgtt agcactattg attattaatt
     3001 tcttatttgt ttcatcttat gaaa
//
LOCUS       DAOMTA010000323         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1343_length_3022_cov_2.007078, whole genome shotgun sequence.
ACCESSION   DAOMTA010000323 DAOMTA010000000
VERSION     DAOMTA010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_3022_cov_2.007078"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1032)
                     /locus_tag="PLK68_08990"
     CDS             complement(358..1032)
                     /locus_tag="PLK68_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRM90579.1"
                     /translation="MPFDIKFYIVNHGIWVIRIMIAAFCGCLIGYERSSRNKGAGVRT
                     HAILALGSALIMLVSKYGFIDINEIDGSRIAAQIVSGVGFLGAGVIFVKNGNVSGLTT
                     AAGMWATSGVGMCIGAGLYDIGIITTIILIGLQILFHRGFFLKVTQNNQNIQMEIYYE
                     DYALKDIREVFDKYGIDIHSMKVENLKNKSMFLEMETYVNKGFQKESLVEEMMNKTYF
                     KMLNYY"
     gene            complement(1045..1803)
                     /locus_tag="PLK68_08995"
     CDS             complement(1045..1803)
                     /locus_tag="PLK68_08995"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRM90580.1"
                     /translation="MRIVNIKNFRDIKGYSSQDGFVIKPYMIFRGGALDKLTIKEQNH
                     FVDHLKIKYILDFRDEAEASLAPDKQHENILYERISALKMQSHDQYGFDFGTMLQGEM
                     TKEKYNYLMSYIKAGYKEMAFNNPAYHRLFELLLRNDGYVYFHCTAGKDRTGVAGFLI
                     MIALGMSEEDAIQEYLLSNIYLKESNDELCQQLQIPEKLREECRPLLYVQRELIEIMI
                     QSIRVKYRSYDEFLLQEYNFDNEKRRRLKEIYCE"
     gene            complement(1805..2629)
                     /locus_tag="PLK68_09000"
     CDS             complement(1805..2629)
                     /locus_tag="PLK68_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM90581.1"
                     /translation="MVYADLHVHTAYSDGIHSIEETIKLAKEKGIKVIAITDHDTVFH
                     FEEVQKVCRQNGLETIRGVEMSCYDYDVHKKVHVVGLGLNNHPYHVEKLCQKTLQCRD
                     EYHHELIKILNQKGLNITYEDAKEYAPYNIVFKMHLFLAIVHKYPEYNDINKYRELFM
                     SETSLDVDSKMGYIDIKEGIKAIREDGGIAIIAHPCEYKNYDEIEKYVSYGLQGIEIS
                     HPSMESEDYPLTQELANRFHLIRSGGSDFHNIHLTAIGDFGLTKEQFDELKEEMGR"
     gene            complement(2642..>3022)
                     /locus_tag="PLK68_09005"
     CDS             complement(2642..>3022)
                     /locus_tag="PLK68_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM90582.1"
                     /translation="SKEVLGKGVAIIPVEGKIYAPANGIISATFETKHAIGLTTENGS
                     EILIHVGIDTVKLEGKPFIQYVEKGEYVKAGSLLLEFDLKMIKEAGLDYTTMVVVTNS
                     NDYLEVIPTKLKKVTKTDPVLTII"
BASE COUNT         1003 a          595 c          326 g         1098 t
ORIGIN      
        1 aagagtcata caaatattgt ataaaatgga gtgcactatt tgatttaaaa ttacctgatc
       61 agttctatta taaatcgcct tatatcctcc cctctcttta cttaaattat agataataca
      121 atttttttaa tcaaaatagt ttccttaaat ggaaactatt ttttgccagt actatctaga
      181 taggtattaa aaaatcttga tgattcaaaa taaagaacat ttcaatcttt aaatcattaa
      241 atataatttt caccagttta agccgattca tgcatatatt cagaaatcaa aaaatagttt
      301 tcattaatta tttttatctc aattaacata tataaaaaag aagagatttt ctctcttcta
      361 ataatagttc aacattttaa aatatgtttt attcatcatt tcttctacta gtgattcttt
      421 ttgaaaccct ttattcacat aagtctccat ttctaaaaac atagatttat tttttagatt
      481 ctcaactttc atagaatgta tatcaatacc atatttatca aaaacttcac gaatatcttt
      541 caatgcataa tcttcataat agatttccat ttgtatgttt tgattatttt gtgtgacctt
      601 taaaaagaac cccctatgaa acaatatctg taatcctatc aaaataattg ttgttataat
      661 tccgatatca tataatcctg caccaataca cattcctact ccagatgttg cccacattcc
      721 agcagcagtt gttaaacctg aaacattacc attctttaca aaaataacgc ctgcgcccaa
      781 aaaaccaaca ccgcttacga tttgggcagc tatacgagaa ccatctattt catttatgtc
      841 tataaaacca tattttgata ctaacattat taaagcagac cccaaagcaa ggatagcatg
      901 tgtacgcacc cccgccccct tatttcgact acttcgttca tagccaatca aacagccgca
      961 aaatgcagca atcataattc ttataaccca tattccatga tttactatat agaatttaat
     1021 atcaaatggc aaaattccca ccacctattc acaatatatt tctttcaatc ttcttctttt
     1081 ttcgttatca aaattatatt cttgtaataa aaattcatca taacttctat atttaacccg
     1141 aattgattga atcattattt caatcaattc tctttgaaca tataataagg gtctacattc
     1201 ctcccttaat ttctcaggaa tttgtagctg ctgacataat tcatcattac tttcttttaa
     1261 ataaatattg gataacaagt attcttggat tgcatcttct tcactcattc ctagagcaat
     1321 cataatcaaa aagccggcaa ctccagtcct atcttttcca gcagtacagt gaaaataaac
     1381 atagccatca tttcttaata acaattcgaa caagcgatga tacgcaggat tattaaaagc
     1441 catctcttta tatcctgctt tgatataaga cattagatag ttgtattttt ctttagtcat
     1501 ctctccttgc agcatagtac caaaatcaaa accatattga tcatgcgatt gcattttaag
     1561 agcactgata cgttcatata aaatattttc atgctgctta tccggtgcta agcttgcttc
     1621 agcttcatct ctaaaatcaa gaatatattt tatttttaaa tgatcaacaa aatgattttg
     1681 ttcttttata gttaatttat ctaaagcacc acccctaaaa atcatgtagg gttttattac
     1741 aaaaccatct tgagagctat atcccttaat atctctaaaa tttttaatat taactattcg
     1801 catcttatct acccatttcc tctttcaatt catcaaattg ctctttagtt aatccaaaat
     1861 caccaattgc agttaaatgg atattatgga aatcactgcc accacttctt ataagatgaa
     1921 atctatttgc aagttcctgt gttaaaggat aatcttcaga ttccatacta ggatgtgata
     1981 tttcaattcc ctgtaagcca tagctgacat atttttcaat ttcatcataa tttttatatt
     2041 cacaaggatg ggcaataata gcaattcctc catcctcacg aattgccttt atcccctctt
     2101 taatatcgat atatcccatt ttagagtcta catctagcga agtctcgctc ataaacaatt
     2161 ctcgatactt attaatatca ttatactcag gatacttatg aacaatcgcc aagaataaat
     2221 gcattttgaa gacaatatta tacggagcgt attctttagc gtcttcgtaa gtaatattta
     2281 agcccttttg atttaatatt ttaataagtt catgatgata ctcatcccta cattgtaaag
     2341 tcttttgaca tagtttttca acatgatacg gatggttatt taaccccaaa ccaacaacat
     2401 gcaccttttt gtgtacatcg tagtcataac agctcatttc aaccccacga attgtttcta
     2461 aaccattttg ccggcatacc ttttgtactt cttcgaaatg aaaaacagta tcatgatcag
     2521 taattgcaat cactttaatt cctttttcct tagctaattt gatcgtttct tcaatagaat
     2581 gaataccatc actataagca gtgtgaacat gtaaatctgc atacaccata ttatcacatc
     2641 cttaaataat agttaaaact ggatcagttt ttgttacttt tttcaatttt gttggaataa
     2701 cttctaaata atcattagag tttgttacaa caaccatagt tgtatagtct aagcccgctt
     2761 ctttaatcat ttttaagtca aattctaata acaatgaccc cgcttttaca tactcacctt
     2821 tttcaacata ttgaataaat ggttttcctt ctaatttaac ggtatcaatt ccaacatgaa
     2881 ttagaatttc agagccattt tcagttgtta agccaattgc atgttttgtt tcaaaagttg
     2941 cacttataat cccattagca ggagcataga tttttccttc tacaggaata atagcaacac
     3001 ccttccctaa aacttctttt ga
//
LOCUS       DAOMTA010000324         3011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1349_length_3011_cov_2.661705, whole genome shotgun sequence.
ACCESSION   DAOMTA010000324 DAOMTA010000000
VERSION     DAOMTA010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_3011_cov_2.661705"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLK68_09010"
     CDS             complement(<1..659)
                     /locus_tag="PLK68_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRM90583.1"
                     /translation="MNLSISEQICYSTIRIESQTSEGVCTGTGFFFNFLNNPVDNTHV
                     PCIVTNKHVVDGGIIGTLVVTCKTEDGMEYLHRVQVSNFRQSWIFHPSANVDLCIMPI
                     QPIVEMLKAKKIQPFFIPLDGNLIPSQSQLNDLSALEDIVMIGYPDGIWDAVNNKPIL
                     RKGITATHPKLDFNGDKKFLIDAACFPGSSGSPVLIINQGGYVDKQGNLNWGKNRILL
                     LG"
     gene            complement(1781..2653)
                     /locus_tag="PLK68_09015"
     CDS             complement(1781..2653)
                     /locus_tag="PLK68_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90584.1"
                     /translation="MGKLTDKIITEFVSQYGDNFNSIDEINSKMQQFMTQCNNVGRDE
                     FEGYSPVEMSDILYCTFSTGNIVQLKECSSDVYNESPLFRQIKYVISVLQRDGKIKLT
                     TKGALPSKLVKELYPLGALDYWVETGLTKVYKEADTISTQLTHLIMKQMKIVKETHGV
                     MTIVKGRTKMLEDDHALMKSMLYVMTNLFNFAYFDLMEENLGVVGIGYSIVLLHKYGD
                     KKRDATFYSTKYFKALFPGEDDHYVYWIRVFDRCLRQLGLIAYKERRFCESPDIQKTD
                     LFDKLITVAAPKGK"
BASE COUNT          997 a          537 c          453 g         1024 t
ORIGIN      
        1 ccaagcaaaa gaattctatt tttcccccaa ttcaaattac cttgtttatc aacatatcca
       61 ccctggttga ttattaaaac aggagaacca ctagaaccag gaaaacatgc agcatcaatt
      121 aaaaacttct tatctccatt gaaatctaat ttaggatgag tagcagtaat tcccttcctt
      181 aaaatcggtt tgttatttac agcatcccaa atgccatcag gataacctat catcacaata
      241 tcttctaatg cagataagtc atttaattgc gattgagaag ggattaaatt accatctaga
      301 ggaataaaaa aaggctgtat ctttttagct tttagcattt ctactattgg ctgaataggc
      361 attatacaca agtctacatt tgctgacggg tgaaaaatcc aagactgtct aaaattggat
      421 acctgaactc tatgtaggta ttccatacca tcttctgttt tgcatgttac aactaatgta
      481 ccaattattc ccccatccac gacatgctta ttagtcacaa tgcaagggac atgagtatta
      541 tcaacaggat tattcaagaa gttaaagaag aaaccggtcc cagtacaaac gccttcactt
      601 gtttgacttt ctattcttat tgtagaataa caaatttgtt cacttataga taaattcatg
      661 attgtaaact atttaataat gcaaatataa tacttatttg gccaataaat tattttttga
      721 ttttttttat caagtctcat tacaccagcc aaccaccctc cccatcattc aagttaaaca
      781 gactcccctg tgggattatg gtgtaaatta aatgtccatt actcctcctt ccaatttgat
      841 aataagtact gaatagtccg ctacacaact ataaataggg ttcactgaat aatttaacta
      901 gcaaaaaaca tagctgtttt tagggaaaaa gtatgaataa cggcatttct gtagaaaatt
      961 aatgtagtaa actataccaa ttctattttt agcttgtaac gataggtcat taatacgata
     1021 agttgtactt tatatgttaa aactatcaca attgaacgta tatcaatgaa tgatagcgta
     1081 ttaaattaat tcagtaaacc ctaaatatat aagaattatg gaaatcttga aatttttgaa
     1141 acgatgtcta ttgctgaatt taaggcacaa agccgattca cagagtggca acagattctt
     1201 aaaataagat tctttattca caaataaatt gcttaccttt acaccgctat ttagaggtag
     1261 agttaaagac cttcacttat aagttattgt cccaatccct tctctttaag ttcgcgtatg
     1321 accccaacat caaaatggga gatactgcga ttgaaagata tttcataaga tataaaaggt
     1381 aaagatctct cttacaaagg gtcatgtatt acgatctaac atgggatcat ggaaaagtgc
     1441 gctacgaatg tgcgctactc aaattcttat tttttgttac ttcgttgtcc ttcaatgagt
     1501 tcaaaagtat aaagatttta ttccatggcc ccaagcggat cactaagaca aatttagagg
     1561 gattccatta cggagtcctt ttttcgttga atagacttct tatgtcccaa ttcggggtcg
     1621 attcataatt ttgggaattt aacaatttct atgcataaag tttagctact ctgaagagga
     1681 aaaatctggg ccagtttgta tttgctatct tgcgttggcg taactgaacg ctaatagtat
     1741 aggactcata tggactttta ggtttgtaag ccatagtgaa ttacttacct ttgggagctg
     1801 ccactgtaat tagtttatca aataaatctg ttttttgaat atcaggagat tcacaaaatc
     1861 tacgttcttt gtatgcaata agtcccagct gacgtaagca tctatcaaac actcttatcc
     1921 aatatacata atgatcatcc tccccaggaa ataatgcttt aaaatattta gtagagtaga
     1981 atgtagcgtc tctcttttta tctccatatt tatgaagtaa aactatcgaa tagcctatac
     2041 cgacaacacc tagattctct tccatcaaat caaaatacgc aaagttaaat aagtttgtca
     2101 ttacatagag cattgacttc attaaagcat ggtcgtcttc aagcatcttt gttcgtccct
     2161 tgacaattgt cattacccca tgtgtctctt ttactatttt catctgcttc attataaggt
     2221 gtgtgagttg agttgaaata gtatcagcct ctttatatac tttggtaagt ccggtttcca
     2281 cccaataatc taatgcacca agcggataca attctttcac aagttttgag ggtaacgcac
     2341 cctttgtggt caatttgatt ttaccatctc gttgcaatac agatatcacg tattttattt
     2401 gacgaaagag agggctttcg ttatagacat cagaagagca ttcttttaat tgaactatat
     2461 tgcccgtaga aaaagtacaa taaagaatat cactcatctc aacaggtgaa tatccttcaa
     2521 attcatcacg tccaacgttg ttacattgag tcataaactg ctgcatcttg ctgtttattt
     2581 catcgataga gttgaaatta tcgccatatt gactcacaaa ctcagttatt attttatctg
     2641 ttaatttccc cataattata aatttaaaat aaattctagt tgtcgtatca ttgatggaat
     2701 attatgatag gattcttcat ggcataggcc ttgcaaaatt cataaatttc atattcgtca
     2761 ttaagatgaa atacatcttc tatttcccaa ttcgttatca taaataaaaa gaggattaat
     2821 atgttttgat aagagtgcaa agatatttat atttttgtat tatgccaata tttccatata
     2881 agaattttca atgttttatt attggttgca catcaataca attaaaaaca ttaataaatg
     2941 gcaatatatt tattataggc acacgaagag taattcgatt agtaatccca attattttaa
     3001 tgtggccgag c
//
LOCUS       DAOMTA010000325         3010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1352_length_3010_cov_2.215228, whole genome shotgun sequence.
ACCESSION   DAOMTA010000325 DAOMTA010000000
VERSION     DAOMTA010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_3010_cov_2.215228"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLK68_09020"
     CDS             complement(<1..202)
                     /locus_tag="PLK68_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRM90585.1"
                     /translation="MVEKFDIVITPLGLERTIHVYLPEDYYDSDEQYPVMYMFDGHNL
                     FYDYDATYGKSWGLKEFLDTYDK"
     gene            complement(225..1394)
                     /locus_tag="PLK68_09025"
     CDS             complement(225..1394)
                     /locus_tag="PLK68_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM90586.1"
                     /translation="MYFEVVKVKYRELGRTGLKVSEIGLGCEGFMKMDTDQVKKFVAQ
                     ASKMGINFIDFFSSNPHGRSAFGKAIAGNREKWIIEGHICTFWKNEQYLRTRKMDEVK
                     AGFEDLLARLQTDYIDIGMIHYIDAKKDFEEVFNGEVIEYVKDLKKRGIIKHIGISSH
                     NPLIAIEAVKTGLVDVILFSINPCYDMLPPSENVDDLWDDERYAEPLFNIDPVRQELY
                     ELCQTKGVALTVMKAFGGGDLLDEKLSPFKVKMTPLQCIHYCLTRPGVASIMAGSHSI
                     KEMMEALAYEDASMVEKDFANVLANIPKHSFEGNCVYCGHCAPCVKGINIADVNKFAD
                     LCVAQGEVPETVREHYEVLAHHASECIECGVCIKNCPFNVKIIEKMKAAVAIFGY"
     gene            complement(1659..2141)
                     /locus_tag="PLK68_09030"
     CDS             complement(1659..2141)
                     /locus_tag="PLK68_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HRM90587.1"
                     /translation="MIKIDEIKVPSTRYHKIVNLLCIIMLTGTLVFLVTNWTMIPDQI
                     PGHYDSAGVINRWTSKSGLWIVYFVGIIMFAGLSVIEHFPKTWNTGVAITKENQKHVY
                     QLIKGMIVTLKLCITTIFMFLTLYTTLMINLPKWFTPLFLIITFGSMIVYGIMIYRAR
                     "
     gene            complement(2211..2981)
                     /locus_tag="PLK68_09035"
     CDS             complement(2211..2981)
                     /locus_tag="PLK68_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM90588.1"
                     /translation="MIKAIFFDIDGTLLSFKNHQIPASTIESLKKLKEKGIKIFVATG
                     RGKDGLDILDEIEFDGYITLNGQYCYVDNQIIYENTIKRDDLQKLLNYLEDHPFPCGF
                     TEEHNKYFNLRDARVDEIHRITLNDDHPAGDCSDVVNKKIYQCMCFIDEREEQKLMQI
                     MPNCISARWHPLFCDVSPKGGTKQNGIDKFLDFYHIDRNETMAFGDGGNDIEMLQHVA
                     LSVAMENGNDKVKEIADYVTADVDEDGILKALQYFSIL"
BASE COUNT         1013 a          609 c          336 g         1052 t
ORIGIN      
        1 ttttatcata ggtatcaaga aattccttta gtccccacga ttttccataa gtagcatcat
       61 agtcataaaa taaattatgt ccatcaaaca tatacatcac tggatattgt tcatcactat
      121 cataataatc ttctggtaaa tatacatgga ttgttcgctc taatccaagt ggtgtgatta
      181 ctatatcaaa cttttcaacc ataatgtttc ctcacgcaat agctttaata accaaaaatt
      241 gctactgctg ctttcatttt ttcaataatc ttaacattga atgggcaatt ttttatacat
      301 accccacatt caatacattc acttgcatga tgagctaaaa cttcataatg ttctctaact
      361 gtttctggta cttccccttg agcaacacat aaatctgcaa atttatttac atcagctata
      421 ttaatgcctt tgacacatgg agcacaatgt ccgcaatata cacaatttcc ttcaaatgaa
      481 tgttttggta tattagctaa aacattagca aaatcttttt ctaccatact tgcatcttca
      541 taagctaatg cctccatcat ctctttaata ctatgacttc ctgccatgat cgaagctact
      601 cccggtcgcg ttaaacaata atgaatacac tgtaacggtg tcattttaac cttaaacggc
      661 gataactttt catctaataa atctccccct ccaaatgctt tcatcacagt taatgcaact
      721 cctttagttt ggcacaattc atacaattct tgacggactg gatcaatatt aaaaagcggt
      781 tccgcatacc tttcatcatc ccacaaatca tcaacatttt cagatggagg caacatatca
      841 taacaaggat taattgaaaa cagtattaca tcaactaatc ctgtcttaac agcttcaatt
      901 gcaataagtg gattatgaga actaattcca atatgtttaa taattccacg tttttttaaa
      961 tcctttacat actcaattac ttcaccatta aaaacttctt cgaaatcttt tttagcatca
     1021 atgtaatgga tcattccaat atcaatatag tcagtctgca gtcttgctag caaatcttca
     1081 aacccagctt ttacttcatc cattttacga gttctcaaat actgttcatt tttccaaaaa
     1141 gtacatatat gcccttcaat aatccacttt tcacggttac cagctatcgc tttaccaaaa
     1201 gcactccgcc catgtgggtt tgatgaaaaa aaatctataa aatttattcc catttttgaa
     1261 gcctgagcaa caaatttctt aacttgatca gtatccattt tcataaatcc ttcacagcct
     1321 aaacctattt cactaacttt taaacctgtt cttcctaatt cccgatattt cacttttacc
     1381 acctcaaaat acattgtact acctaaagtg aactttaggt caaccattat ttatgataaa
     1441 aggatatatt tctgtaaact atcgtctaca gaaaagacat aaaatataaa ggaatgctat
     1501 caatatatta tttttgtacg aaaaaaatgt attagacacg gaaataattt tttacagaag
     1561 aacactatta aaagcgatag ttcaatttat tattaaaaac aaatatcatt ttcgtatttt
     1621 taataagaga ttaatacacc cttaattatt aggtaatttc atcgagctct gtaaatcatt
     1681 ataccataaa ctatcattga accaaatgta ataattagaa aaagcggtgt aaaccatttt
     1741 ggcaaattaa tcatcaaagt cgtatacagt gttaaaaaca taaatattgt ggtgatacat
     1801 aatttcaggg ttactatcat tccttttatt aattgataaa catgtttttg attttcctta
     1861 gtaatagcta caccagtatt ccacgtttta ggaaaatgtt ctattactga taatcccgca
     1921 aacattatga ttccaacaaa ataaactatc catagccctg acttacttgt ccatcgattg
     1981 attactccag cactatcata atgtcctgga atttgatccg ggatcatcgt ccaattagtt
     2041 accaaaaaga ctagtgttcc tgtcaacatt attatacaaa gaagatttac tattttatga
     2101 tatctagttg aaggcacttt aatttcatct attttaatca cctcttacaa acaatattat
     2161 actcccttga aatatcactt tcatcattaa tgataagtca tcatcttatt ttaaagaata
     2221 ctgaaatact gcagtgcttt taaaattcca tcttcgtcta catcagcagt cacataatca
     2281 gcaatttctt taactttatc attaccattt tccattgcca ctgataatgc cacatgttgt
     2341 aacatctcaa tgtcattccc accatcacca aaagccattg tttcattacg atcaatatgg
     2401 taaaaatcta aaaacttatc aattccattt tgttttgttc ctcctttggg agaaacatca
     2461 caaaaaagtg gatgccatcg cgcagaaata caattaggca taatctgcat taatttttgt
     2521 tcttcccgct catcaataaa gcacatacat tgataaattt ttttattaac tacatcacta
     2581 caatcaccag ctggatgatc atcattcaaa gtaatgcgat gaatttcatc aactcgagca
     2641 tcacgaagat taaaatattt attatgttct tccgtgaacc cacatggaaa aggatggtct
     2701 tctaaataat tcaaaagttt ctgtaaatca tctcgtttaa ttgtattttc atatattatt
     2761 tgattatcaa cataacaata ctgaccattt aaggttatat agccatcaaa ctcaatctca
     2821 tctaaaatat ctaaaccatc ctttccccgt cctgtagcaa caaaaatttt gattcccttt
     2881 tcttttagct tctttaacga ctcgatagtt gaggcaggta tttgatgatt tttaaaactc
     2941 aatagtgttc catcaatatc aaaaaatatc gcttttatca tttctgtttc tcctttttct
     3001 aattaatatg
//
LOCUS       DAOMTA010000326         3010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1353_length_3010_cov_2.171912, whole genome shotgun sequence.
ACCESSION   DAOMTA010000326 DAOMTA010000000
VERSION     DAOMTA010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1353_length_3010_cov_2.171912"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLK68_09040"
     CDS             complement(<1..826)
                     /locus_tag="PLK68_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90589.1"
                     /translation="MKEVIVSAGLVYIYLNGYLENERLHIQSIDTYSCLENGFYQRVI
                     EASTIANEVDEIVHIFAVVGVKEKANQIYSMIEKRLKNNFNNIIAGKNIKKSSKMLDK
                     TGNVFFRCVQEFFCEQLDHFPKQVNYDFNDFTIKGICSSRLPDYVGDSITNEIIYIWL
                     EREKPMLCGRQLMIRSFFVRDFIGRKVIASLPNKENESWRFIFEGGHQISMNEKFSYL
                     KETLHPDDLGGFCSSNIQSILMNPIYAYGQWFQPNDVCEEWHKVFLYLCAISDNEWN"
     gene            2312..>3010
                     /locus_tag="PLK68_09045"
     CDS             2312..>3010
                     /locus_tag="PLK68_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HRM90590.1"
                     /translation="MILEQINPDLLTLAKESDTVLHDIYEKIDSICLTNSNRILSAFI
                     ENKVSYSDFSDINGYGNYDEGRNKIEKIFATVLGCEDALVRPQIMSGTNALYITLSAL
                     LKHGDTMISLSGAPYDSLQEMIGISGDSSQSLKAAGVKYEQIELINDDFDDQMIIERL
                     KEKNIKLVEIQRSRGYSHRKSLSIAKIERIIKKIREVNDEVIIMVDNCYGELVETKEP
                     GHVGADIVVGSLMKN"
BASE COUNT         1093 a          461 c          364 g         1092 t
ORIGIN      
        1 cattccactc attatctgaa atagcacata gataaagaaa cactttatgc cattcttcgc
       61 aaacatcatt tggttgaaac cattgaccat atgcataaat tgggttcatt aatatacttt
      121 gtatatttga tgaacagaat ccacctaaat catctggatg taatgtttct tttaaataag
      181 aaaatttctc attcattgaa atctgatgac ccccttcaaa aatgaatcgc catgattcat
      241 tctctttgtt aggaagtgag gctataactt ttctcccaat aaaatcacgc acaaagaaag
      301 atcttatcat caattgtcta ccacacaaca ttggtttctc gcgttctagc catatataaa
      361 taatttcatt cgtaatagaa tctcctacat aatccggtaa gcggctagaa caaattcctt
      421 tgatagtaaa atcgttaaaa tcgtaattaa cttgtttagg aaaatgatct aattgttcac
      481 aaaaaaactc ttgaacacag cgaaaaaaaa cattacctgt tttatctagc attttcgaag
      541 attttttaat atttttccca gctataatat tattaaaatt attctttaat cttttctcta
      601 tcatagaata tatttgattt gctttttctt taactccaac aacagcaaaa atatgtacta
      661 tttcatcaac ttcattagct attgttgaag cctctattac acgttgataa aagccatttt
      721 ctaaacagct ataagtatca atggattgaa tatgcagcct ttcattttct aaatatccat
      781 taagatatat gtatactaat cctgctgaaa cgattacttc tttcataatt tcatcaccat
      841 ccttaaaaaa ttttaaatct tcatcataat aatagcacat taattttgta tcctatttca
      901 taaaaaacag atgtatctga tttacatatg gcagctctta ggactataag tccatcaaat
      961 tttctatact caatattctc tttttacaaa aaatacttct aataaccttt atttaataca
     1021 acttatcatt gtagttatag aatatatcat tttttattat ataccacata tgttctcatt
     1081 gcttaaaaaa taaatatttt taccaacttg tcttattagt ttagacagtc actcttttgt
     1141 aatcatattt cttttatcta tatatttata tttcaatatt attctaaacg actattaaaa
     1201 attaagcact caaaatattc taaaaaatat aaaactattt ctatagatat tagtatcaca
     1261 taatttaact atctaatagg caatggtggg ataatcatac ctaaaaacca aatattatag
     1321 aaataattag catcataata atagctccta ccttttgagc attactcttg aaagtattca
     1381 taaacggaac aaatagcatt actaaaaatg gtagaaacgg accagatatg gattttatat
     1441 tattatctat gacttctcaa ctatcactgt gatgattttt catcttatcc aaaatatcat
     1501 catactctct cttagttata tctttagatt ttaataaatt aaaatctaaa ctagatattt
     1561 ttttaagtaa atctactctt ttattcatat caatataata taaataatta ctttctatat
     1621 aatgaaaact tatactcata atgtacaaaa caataataat caaatataaa atgaaggctt
     1681 tcgaaaattg acttttattg catcaaaaaa taattttcaa aatatatctc attgatcttt
     1741 ttaacctcaa cttcttctat aaattttaca aagggtttgc tttgctaagc taagcacaat
     1801 tattatgcaa attattgata ataacttgat atcacaagct agtaccattc aatatcgttt
     1861 taataatttg taatcaatat tttaaagcgc atatctatca tattcttatt cgaactcgtc
     1921 aaattcatac aaatttacta atctttttaa attaagatat aagcgctact tctcttaatt
     1981 tataaatttt caataataat ccatatttca aaatctaata aagtcaattt aaagcattga
     2041 ccccgtaagt atttaaagct atttaatttt tatcccgcaa ttttttagag agctcaatta
     2101 agagcgtcca tggtgtttaa tttgattaaa ttttcaactc atcatctaat ttataaatat
     2161 taatgggtag aaaaaattca tcagtcatca tttgttgctc attaatatga gcactcaatg
     2221 atagatagat tatagttaat agatattata ccaatgcaat taatcaaaaa atgtgtataa
     2281 taaacaatgt attatttata ggaggttttt tatgatactt gaacaaataa atccggatct
     2341 acttacttta gctaaagaat ctgatacagt tttacatgat atctacgaaa agatcgactc
     2401 tatttgtcta acaaattcta atcgaatctt atctgctttt attgaaaaca aagtctctta
     2461 ctcagatttt agtgatatca acggttatgg aaactatgat gagggaagaa ataagattga
     2521 aaagattttt gcaacggttc ttggttgtga ggatgcatta gtacggcctc aaattatgag
     2581 cggaacaaat gccttataca taactctttc agccttattg aaacatggtg atacaatgat
     2641 ttcattgtct ggtgctcctt acgattcgct tcaggaaatg attgggataa gcggagattc
     2701 aagtcagtcg ctaaaagctg ccggagtgaa gtatgaacaa attgaattaa ttaatgacga
     2761 ttttgatgat caaatgatta ttgaacgctt aaaagaaaag aatattaaat tagttgaaat
     2821 tcaaagatct aggggttatt cgcatcgtaa gtcattatcg atagcaaaga ttgaacggat
     2881 cattaaaaag attcgtgaag taaatgatga agtaattatt atggtagata attgttatgg
     2941 cgagttggta gaaaccaaag aacccgggca tgttggtgct gatattgttg tcggatcttt
     3001 aatgaagaat
//
LOCUS       DAOMTA010000327         3001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1360_length_3001_cov_2.207060, whole genome shotgun sequence.
ACCESSION   DAOMTA010000327 DAOMTA010000000
VERSION     DAOMTA010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1360_length_3001_cov_2.207060"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLK68_09050"
     CDS             complement(<1..316)
                     /locus_tag="PLK68_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90591.1"
                     /translation="MEQMLEKWYEFFDKERLNKWIDEDKENRSGFIAGVNEKTINEIR
                     NIFENNIEEFIEFYDYTGGQILLPIFKIRDQYREFKERKVSNEYGYYVRDIPTSESSD
                     IPF"
     gene            complement(364..651)
                     /locus_tag="PLK68_09055"
     CDS             complement(364..651)
                     /locus_tag="PLK68_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90592.1"
                     /translation="MKEIITMEVIKITTMGSFDPLIQLVTPFAEDALLFFQTMAVLFA
                     TAMGVYYKLRQIFADAQEDQMWDKKTRGVFVGLVFIFAIPTVIKVLSSYFN"
     gene            complement(683..1456)
                     /locus_tag="PLK68_09060"
     CDS             complement(683..1456)
                     /locus_tag="PLK68_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90593.1"
                     /translation="MRIKLNKKLLVRKEDGSVNRITINQKDYYKFILPKGCDFGNTLD
                     ENGNEVGKLPDSIRDSFIVPVWYTSQAIEGELCYIDFPDNYKYLKITLDLGKSEERLE
                     DGRHKHLFSAIENISPNELADIIEDTKWLSFTVSVKQLGKPYQTEQGNKRISILLPKY
                     AGDLMGCRATISQNCIKDIKGRDDIKIVNIPKNSKFNIMRSKIVGQDIENQMKPVFGD
                     KIIEATVTGKELFELFKKPNEYEEQTTHEVESEEMEQGL"
     gene            complement(1469..1639)
                     /locus_tag="PLK68_09065"
     CDS             complement(1469..1639)
                     /locus_tag="PLK68_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90594.1"
                     /translation="MNIIGIICGVVVAVGCLIVFLCQLIKNDIENVIVFIVTRLAIAI
                     AAGLTVAMALSI"
     gene            complement(1707..2771)
                     /locus_tag="PLK68_09070"
     CDS             complement(1707..2771)
                     /locus_tag="PLK68_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM90595.1"
                     /translation="MKKRYVVIFLLILMFFISGCQSELFSDPNFSKDKIKFNSNIVIN
                     YGEKNVDTTKFVSGIDDIKIKDSNRLKDELRITVDNYSIICPEFDADELGKHVLVYSL
                     GDYKYKVEVTVKDITAPLILLQNNEIDLTVGEKFSKKNLDYKIKDNLSKKKDIRISIK
                     GDYNSKKLGKYNLKLVAKDEAGNKSSVDFVLNIYEKASLEIDKTNISLNIGETSLVSA
                     KVKGKDQTVNWTSDNNTIVVVDNGKITAVAPGTAIIRVTCGNLAKEINVNVKAKETNN
                     NANSQNSNNNKNNANNSSSGGTGGNKVTNPSQYNKYFSGVSIATYNEALSYAEGKVNS
                     GVVNSYSLMPDGKGYQVTFG"
BASE COUNT          944 a          577 c          322 g         1158 t
ORIGIN      
        1 agaatggtat atcactagat tcgcttgttg gaatatccct aacataatat ccatattcat
       61 tagatacttt tctctcttta aattctctat actgatctct tattttaaaa attggtaata
      121 atatttgacc accagtatag tcataaaatt caataaactc ttcaatatta ttttcaaaaa
      181 tattcctaat ttcattgatt gtcttttcat taacccctgc aataaaacca gatcgatttt
      241 ccttatcttc atcaatccac ttattgagtc tttctttatc aaaaaattca taccactttt
      301 ctaacatttg ttccaattct atacctcctt tcaaaaatat aaaaagaggc aaattgcctc
      361 tttctaatta aaatatgaag ataatacttt gattactgta ggaattgcaa aaataaaaac
      421 cagtcctaca aatacccctc ttgttttctt atcccacatt tgatcttcct gtgcatcagc
      481 aaaaatttgt cttaatttat aatatacacc catagctgta gcaaataata ctgccattgt
      541 ctgaaagaac aaaagcgcat cttcagcaaa aggggttaca agttgaatta atggatcaaa
      601 tgaccccata gtagttattt tgataacttc cattgtaata atttctttca taaagtctcc
      661 tttctaaaac tacacaaaac cgttataatc cttgttccat ttcttcactt tccacttcgt
      721 gagtagtttg ttcttcgtat tcattaggtt ttttaaacaa ctcaaataat tcctttcctg
      781 ttactgttgc ttcgataatt ttatcgccaa atactggttt catttggttt tcaatatcct
      841 gaccaacaat tttagaacgc attatgttaa atttagagtt ttttgggata ttaacaattt
      901 taatatcatc tcttcctttt atgtctttga tacagttctg actgatagtt gctctgcacc
      961 ccatcaaatc acctgcgtat tttggaagta aaatgcttat ccttttattt ccctgttcag
     1021 tttgatacgg tttacctaac tgttttaccg aaacggtaaa acttaaccac tttgtatctt
     1081 caataatatc tgcaagttca tttggcgaaa tattttcaat tgcagaaaaa agatgtttat
     1141 gtctaccatc ttctaatctt tcttcgctct tgcccaaatc taaagtaatt tttaaatact
     1201 tatagttatc aggaaaatca atataacata attcaccttc tattgcctga cttgtatacc
     1261 agacaggtac gataaatgag tcacgtattg aatctggtaa ttttcctact tcatttccat
     1321 tttcatctaa tgtatttcca aagtcacatc cttttggcaa aataaattta tagtaatctt
     1381 tttgattgat tgttatacgg tttactgaac catcttcctt tcttactaat aattttttat
     1441 ttagtttaat tcgcataatt attcctcctt aaattgataa tgccattgca acagttaagc
     1501 cagctgctat agcaatagcc agtcttgtta caataaatac aattacattt tcgatatcat
     1561 ttttaatcag ttgacataaa aaaacgataa ggcatcctac tgcaacaact acaccacaaa
     1621 taattccaat aatattcatt tgctttttct cctttcaaaa tataaaaata tataataaaa
     1681 aatccattga catggatttt tattatctaa ccgaatgtta cttgatatcc cttgccatct
     1741 ggcattaatg aataagaatt tacgacccca ctgttcactt taccctcggc ataacttaat
     1801 gcttcattgt atgtagcaat cgaaacccca gaaaaatatt tattatactg tgagggattg
     1861 gtaaccttat tgccacctgt cccgccactc gaactattat ttgcattatt tttattatta
     1921 tttgaatttt gactgttagc attgttgttt gtctctttag cttttacatt tacattaatt
     1981 tcttttgcga gatttccaca tgtgactctt ataattgcag tacctggagc cactgctgta
     2041 atctttccat tatcaactac cacaatagtg ttgttgtcgc tcgtccaatt tacagtttga
     2101 tcttttcctt taaccttcgc tgatacaagc gatgtctccc caatatttaa tgaaatatta
     2161 gttttgtcaa tctccagtga agccttttca tatatattca aaacaaaatc aacactcgat
     2221 ttatttcctg cttcatcttt agcaaccaat ttaagattgt acttacccaa ttttttagaa
     2281 ttataatcac cttttattga aatcctgata tctttcttct tggaaagatt atcctttatt
     2341 ttgtaatcta aattcttttt tgaaaacttt tctccaaccg ttaaatcaat ttcattattt
     2401 tgtaatagaa tcaaaggtgc agttatatct tttacagtta cttctacttt gtatttgtaa
     2461 tcacctaaag aataaacaag tacatgttta cctaattcat ctgcatcaaa ctcaggacag
     2521 ataatactgt aattatcaac agtgattctt agctcatcct ttaatctatt gctgtcttta
     2581 atttttatat catctatacc cgacacaaat tttgtagtat caacattctt ttccccatag
     2641 tttattacta tattgctatt aaattttatt ttatctttgc tgaagtttgg atcactgaat
     2701 aactcacttt gacagccact aataaaaaac attaatataa gtaaaaatat tacaacatat
     2761 ctttttttca tattctccac acacctttct ttttttgtat atgccatcta aatttttaaa
     2821 tagcatatac aaaaaagagg caaattgcct cttttagttt aattaacaac tttttttcct
     2881 tctataataa acaagggaca ttcctaaaac tgcaacacct gttaaaacaa gataactaaa
     2941 cggattgctt aaatcaccag tttttacctt tttagcaaca ggcataagtt catcatacat
     3001 t
//
LOCUS       DAOMTA010000328         3000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1364_length_3000_cov_2.048217, whole genome shotgun sequence.
ACCESSION   DAOMTA010000328 DAOMTA010000000
VERSION     DAOMTA010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_3000_cov_2.048217"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLK68_09075"
     CDS             <1..264
                     /locus_tag="PLK68_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRM90596.1"
                     /translation="DYSLLNKPMCFFVPDLDEYIETLGCFVDYRRVMPGAICYNETQV
                     IAALQGNKKYDIKAFRDMFFKYQDGHNTQRIVMFIDKLINTKN"
     gene            complement(279..500)
                     /locus_tag="PLK68_09080"
                     /pseudo
     CDS             complement(279..500)
                     /locus_tag="PLK68_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538090.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     assembly_gap    575..584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<585..1030)
                     /locus_tag="PLK68_09085"
     CDS             complement(<585..1030)
                     /locus_tag="PLK68_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM90597.1"
                     /translation="MITAVIFDMDGLMIDSERVTFEGYKHVLAKHNLTLSLEAYKTLL
                     GKPVKAVYELFHKDYGDDFDVEETIKAVHQYMADLFENEGVPLKEGLIELLKYLKEND
                     YKTIVATSSQRHRVDHILELSGLQKYFDDSICGDEVTKGKPDPEVF"
     gene            1139..1474
                     /locus_tag="PLK68_09090"
     CDS             1139..1474
                     /locus_tag="PLK68_09090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM90598.1"
                     /translation="MIHRGEIYYADLSPVVGSEQGGYRPVIVLQNNKGNRYSTTVIIA
                     PISSRLTKNPLPTHVMVDCSSLEKKSVVLLEQIRTIDKQRIKEKVGMIDSQVMNLINQ
                     AIKTSLDIK"
     gene            1528..2376
                     /locus_tag="PLK68_09095"
     CDS             1528..2376
                     /locus_tag="PLK68_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRM90599.1"
                     /translation="MNVRDIKLKAKSVLVNRNNIVTVFVFISVITTLANYIGSEIGGV
                     IPFLSLIVTIIMLPFGHGNVVTALKTVNEQGDEVTIDQDGVVGLRRFKDLFGTYFIRE
                     ILLLVIMMLLGLIIFVIARFTVDSNVFSQLGAIIEQAAVYSTNVSAYLNDPTVIEALG
                     TLGVIFFIGIIIIAIVAFIYSLVFALTPFVLEKYNIRGAKAMSESARLMKGHKRTLFM
                     LYLSYLGWAILVLVLSAVVEMILPIPLILNIIVAALTTYLFGAELNTSLAVFFEEIDL
                     EDKNNI"
     gene            2687..>3000
                     /locus_tag="PLK68_09100"
     CDS             2687..>3000
                     /locus_tag="PLK68_09100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRM90600.1"
                     /translation="MVYLFSIAGELIVNLVAPQSGEAANQTLVETLVQSNFLLMFFQS
                     VIIAPFIEELLFRGLIFNSLRQKNMVWAHLISAFLFGLLHVYSYILAGDMSEWIKLIP
                     YM"
BASE COUNT          992 a          406 c          517 g         1075 t
ORIGIN      
        1 gattattcct tattaaataa accaatgtgt ttttttgttc cggatttaga tgaatatatt
       61 gaaacattag gatgttttgt ggattatcga agggtcatgc ccggagcaat ctgttataat
      121 gaaacgcaag ttattgcagc cttgcaaggt aataaaaaat atgatattaa ggcatttaga
      181 gatatgtttt ttaaatatca agatggtcat aatacacaaa gaattgttat gtttatcgat
      241 aaattaataa acaccaagaa ttaattcttg gtgtttattt aatgattttc attcgccaaa
      301 taatcacgga catttgataa attgtccatg atcttattag tcattatcgc aaacttatga
      361 tcagggtatt ttaaatcagg aatacaaatt actttaatgc ctgcactata ggcagcgtta
      421 attccggatt cagaatcttc gagaaccaga gcttcatcgg gcgtaattcc taatttttgg
      481 catgatttta aaaatacttc aacaagcaat tatgcacatg cttaaaacaa ataaagaaat
      541 taaaagcctc agcaaagaaa taaaaagtct ctgcnnnnnn nnnnaaaaat acttcaggat
      601 caggttttcc ttttgttact tcgtcaccac aaatggaatc atcaaaatat ttttgtaaac
      661 cagataattc taaaatatga tcaacacgat gacgctgtga actggttgca acaattgttt
      721 tatagtcgtt ttcttttaaa tactttagta actcaattaa tccttctttt aaaggaaccc
      781 cttcattttc aaataagtca gccatatatt gatgaactgc tttaattgtt tcctcaacat
      841 caaaatcgtc gccataatct ttatggaaaa gctcatagac tgctttaaca ggctttccta
      901 aaagagtttt ataggcttct aatgataatg ttaaattatg tttagctaat acatgcttat
      961 atccctcgaa tgtaactctc tcactatcaa tcattaaacc atccatatca aaaattactg
     1021 ctgttatcat ctttttacct ccacgcaaat atcatatcat aattatccga ttagtaaaaa
     1081 gattttcata gaataaaaaa agttctaatt catatactgc actaaaggag ttgtagatat
     1141 gatacataga ggggaaattt attatgctga tttatctcca gtagtgggga gtgaacaagg
     1201 aggatatcga cctgtgattg ttttacaaaa taataagggc aatcgatatt cgacaacagt
     1261 gattattgcg ccaatttctt cacgattaac aaaaaatccg ttaccaaccc atgtgatggt
     1321 tgattgcagt tcattggaaa aaaagagtgt agttttacta gaacaaatta gaacgattga
     1381 taaacagcga attaaagaaa aagttggaat gattgatagc caagtaatga atctgatcaa
     1441 tcaagcaatt aagacaagct tggatattaa atagtttcct tttgagtcag atatttgcta
     1501 taatagcccc atggaggtag aggaaagatg aatgttcgtg atattaaatt aaaggctaaa
     1561 agtgttttag ttaatcgtaa taatatagtt acagtatttg tgtttattag tgtaataaca
     1621 actttggcta attatattgg ttcagaaatt ggcggggtga taccatttct ttcattgatt
     1681 gtaactatta taatgttgcc ctttggtcat gggaatgtgg taactgcatt aaaaacagtc
     1741 aatgaacaag gtgatgaggt cactattgac caagatgggg tagtcggatt aagacgtttt
     1801 aaagatttat ttggcacata ttttattaga gaaatattat tattggtgat tatgatgctg
     1861 cttggattaa tcatattcgt aattgctaga tttacagtag acagtaatgt tttttctcaa
     1921 ttaggagcaa ttattgagca agctgctgta tactctacta atgtaagtgc atatttaaat
     1981 gatccaactg taattgaagc attaggaaca ctaggagtaa tcttttttat tggtatcatc
     2041 attattgcaa ttgtagcgtt tatttatagt ttggtatttg ctttaacacc ttttgtatta
     2101 gaaaaatata atatacgcgg tgccaaggca atgagtgaat cagctcgttt aatgaaagga
     2161 cataaacgaa ctttatttat gctgtatttg tcatatttag gatgggctat cttagtatta
     2221 gtactttctg ctgttgttga gatgatttta ccgattccat taattttgaa tattatcgtg
     2281 gcggctttga ctacttattt gtttggtgct gaactaaata ctagtttagc ggtatttttt
     2341 gaagaaattg atttagaaga taaaaataat atttaacgag gtgttaattt gaatttgata
     2401 aaaaaaatta aattaaatag taggcaaaag gcgatagggg tcatattgat ttttccctgg
     2461 tatctttatt ttgcaccaat tgtaattaat tattttttga agctatatac agtatatata
     2521 gctaatgatt tttcaactaa tagtttaaat gcatttatta atttattcat cggaattgca
     2581 actgctattc ctttgattat tgtgtttaag ggttttatta aagaaaactg gcttgttttt
     2641 aaaaaagact ttttggaaaa tataatatgg gtgcttacga tcgggattgg tttatttatt
     2701 ttcaattgca ggggaactga ttgtcaatct tgtagcccct caatcaggcg aagcagctaa
     2761 ccagacatta gtggaaactc ttgtgcaatc taattttcta ttaatgtttt ttcaatcagt
     2821 aattattgca ccatttattg aagagttgtt atttagaggt ctgattttca acagtttacg
     2881 acaaaaaaat atggtttggg ctcatttgat ttcagcattc ctatttggct tgttacatgt
     2941 ttacagctat attttagctg gtgatatgag tgaatggatt aaattgattc catatatgat
//
LOCUS       DAOMTA010000329         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1374_length_2990_cov_2.163884, whole genome shotgun sequence.
ACCESSION   DAOMTA010000329 DAOMTA010000000
VERSION     DAOMTA010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_2990_cov_2.163884"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLK68_09105"
     CDS             <1..820
                     /locus_tag="PLK68_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM90601.1"
                     /translation="WIVKMLSKQYDLNIAKKICQHDNTPPTRAARVNILKTTKAQLLT
                     DENFEAGNLSPDGLLYRAGNIANTDYFKNGLVTIQDESSQLIAPLLDPQPTDLVLDMC
                     CAPGSKTTHLAAIMNNQGKIIAYDLFEHKIKLVEANLKRLGVNNVELHVGDATLLKEK
                     YSEESFDKILLDAPCSGLGVMKRKPEIKYHDSGAMDTIIPLQAKLLDNAYYLLKKNGK
                     MIYSTCTINKKENEQMIKQFLDKYPDMKIIEEKKILPYVYDSDGFYMCKLEKGK"
     gene            822..1836
                     /gene="rlmN"
                     /locus_tag="PLK68_09110"
                     /pseudo
     CDS             822..1836
                     /gene="rlmN"
                     /locus_tag="PLK68_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
     gene            1836..2573
                     /locus_tag="PLK68_09115"
     CDS             1836..2573
                     /locus_tag="PLK68_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRM90602.1"
                     /translation="MNYYALTDIGKVRNKNQDQATVIANVKDQVIAIVCDGMGGHRAG
                     EIASRVVMDQFVNCFDSIPPFDNVDELKKWVNETIYEADAIVKRMAKQNVEHHGMGTT
                     IVVAILMDNVIYISHVGDSRAYVLKNDQLMQLTKDHTLVNALVDRGAISEEEAVNHSQ
                     KNVLTQAIGADTELTPSFIELEFADSLLLLCSDGLYNCLNDEIIKEILKKNIKVSQKV
                     TELIDRANENGGRDNIGVAIIDNREEK"
     gene            2575..>2990
                     /locus_tag="PLK68_09120"
     CDS             2575..>2990
                     /locus_tag="PLK68_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRM90603.1"
                     /translation="MAKIIAERYELLELIGQGGMADVYLAQDIILNRTIAIKILRTSL
                     AKDPIYVTRFQREASAAAALSHKNIVEIYDVGEDEDKYYIVMEYVPGMTLKELILKRG
                     AVHVVEAIDIMKQVISGISKAHQLGIIHRDLKPQNI"
BASE COUNT         1067 a          381 c          606 g          936 t
ORIGIN      
        1 atggatagtt aagatgttaa gtaaacagta tgatttaaat atagccaaaa agatttgtca
       61 gcatgataat acaccaccaa ccagagcagc gagggtcaat attttaaaga ctacaaaggc
      121 tcaattatta actgatgaaa attttgaagc agggaatctt tctcccgatg gactcttgta
      181 cagagcagga aatattgcta acactgatta ttttaaaaac ggattagtca ctattcaaga
      241 cgagtcaagc cagttgattg ctccgttgct ggatcctcag ccaacggatc tcgttcttga
      301 tatgtgttgt gctccaggaa gtaaaactac acatttagca gctattatga ataatcaggg
      361 taaaataatt gcatatgatt tatttgaaca taaaatcaag ctagttgaag ctaatctaaa
      421 acgcttaggt gttaataatg tagaactgca tgttggggat gccactttgt tgaaagaaaa
      481 atattcagaa gaatcatttg ataaaatttt gcttgatgca ccttgcagcg gtttaggagt
      541 aatgaaacgt aaaccggaga ttaaatatca tgattctggt gcaatggata caatcattcc
      601 tttacaagca aaattgttag acaatgccta ctatttattg aagaaaaatg gtaaaatgat
      661 ctatagtacg tgtacaatca ataaaaaaga aaatgaacaa atgataaaac aatttcttga
      721 taaatatcca gatatgaaga taattgaaga aaagaagatt ttaccgtatg tttatgacag
      781 tgatggattt tatatgtgta aattagaaaa aggaaaataa gatgaaaaat atttatgatt
      841 attcactaga acaactaacg gaatattttg cttcaatcaa acaaaagcca ttccgtgcta
      901 aacaagtttt tagctggctt taccagaaag atgctcgtag ttttgatgat atgtcggatt
      961 tatcaaaaga tctacgaaat caattgaaag tagaattttc tttagatgtt ttaaaaatta
     1021 aagaaaaaca agtatcaaga gatgggacaa ttaaatatct gttcgaatta ttagatggaa
     1081 gtttgattga atctgtctta atgattcatg actatggtaa gtcactatgt gtaactagcc
     1141 aaattggctg caacatgaaa tgtacatttt gtgctagtgg tttattacgc aaacaacgtg
     1201 atttaacacc aggtgaaatt gttgcacaaa ttattaaagt acaacaagat attgatcaac
     1261 gagttagtca tgtagtcgta atggggacgg gtgaaccatt tgataattat gataatgtga
     1321 tggaatttgt acggatcatt aaccatccac atggtctagc tatcggcgct cgacacatca
     1381 ctatttcaac ttgtggattg atcaaaggga tcaagcgata tagtgaagaa ggaattcaaa
     1441 ctaatttggc gatttcgtta catgctgcta atgatgaaat tcgcgatgaa ttgatgccga
     1501 tcaataaggt gcatccaatg gatgatttac gtgaagctat cagtgaatat attgataaaa
     1561 caaatcggcg tgttactttt gaatatatca tgttaaaagg tgttaatgat gatattgttt
     1621 acgctcgtca attggcacac tatctacgtg gattaaatgc atatgttaat ttaataccat
     1681 gagcatggtt atcaacctag tgataaagaa acagtagaaa tatttaaaaa tgagctatta
     1741 agattacata ttaatgtaac tttaagaaaa gaacatgggc gcgatattga tggtgcttgt
     1801 ggacaattaa gggctaaaag aagtggggtg aaataatgaa ctattatgca ttgactgata
     1861 tcggtaaagt acgaaataaa aatcaggatc aggctactgt tattgcaaat gtaaaagatc
     1921 aagttattgc aattgtctgt gatggtatgg gtggtcatcg tgctggtgaa attgcttctc
     1981 gggtggtgat ggatcaattt gttaattgtt ttgacagtat ccccccattt gataatgttg
     2041 atgagttgaa aaaatgggta aatgaaacaa tttatgaagc tgatgcgatt gtaaaaagaa
     2101 tggctaagca aaatgttgag catcatggta tgggtacgac aattgtcgtg gcaattttaa
     2161 tggataatgt gatatatatt tctcatgtcg gtgatagccg tgcttatgtt ttgaaaaatg
     2221 atcaattaat gcaattgact aaagatcata ctttagttaa tgctttagtt gatcgtggag
     2281 caatcagtga agaagaagct gttaatcata gccaaaaaaa tgttttgact caggcaattg
     2341 gtgcagatac tgaattaacc ccatctttta tcgaattaga atttgcagat agtttattgt
     2401 tgctttgcag tgatggatta tataattgct taaatgatga aataattaaa gaaattttaa
     2461 aaaagaatat aaaagtatct caaaaagtta ctgaattaat tgatcgagct aatgaaaatg
     2521 gtggacgtga taatatcgga gtagctatca ttgataatcg ggaggaaaaa taggatggca
     2581 aaaataattg cagaacgtta tgaattgtta gaactaattg gtcaaggcgg aatggcagat
     2641 gtttatttag ctcaagatat tatcttaaat cgtactattg cgattaagat tcttcgaact
     2701 agccttgcta aagatccaat atatgtaact agatttcaac gtgaagctag tgcagcagct
     2761 gcactttctc ataaaaatat tgttgaaata tatgatgttg gtgaggatga agataaatat
     2821 tatattgtca tggaatatgt gccgggtatg acgttaaaag agttgatttt aaagcgtggt
     2881 gctgttcatg tagttgaagc aattgatatt atgaaacaag taattagtgg aatttctaaa
     2941 gctcatcagc tagggattat ccatcgtgat ttaaaaccgc aaaatatttt
//
LOCUS       DAOMTA010000330         2987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1375_length_2987_cov_2.681446, whole genome shotgun sequence.
ACCESSION   DAOMTA010000330 DAOMTA010000000
VERSION     DAOMTA010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1375_length_2987_cov_2.681446"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLK68_09125"
     CDS             <1..659
                     /locus_tag="PLK68_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90604.1"
                     /translation="LYNNLNYLVNDAVLQEPSSNDLSYLDNIENNENYNVAITYLNYV
                     EDGLNYLDLISVTRGNDDLLEIRNDSEYYLYIKYLDNGYTNNVLIVPNDTYYCSIIDE
                     EELTIETIAFYNFEDPALDVFTNLSDIDIYMNVDSIDFLETNAEILFKHYYYQAYLKL
                     PYDDIMVIKERDSNKGYYGYLDYTNRQIKVYYSDNYTHDVTIENIQNYQSDYLKTITL
                     "
     gene            675..2099
                     /locus_tag="PLK68_09130"
     CDS             675..2099
                     /locus_tag="PLK68_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM90605.1"
                     /translation="MISDNFFTEHMTIVIAVAIVLLLIIILLTCWKRVPADKALVITG
                     LKKRVLTGKGGIQVPFLETSCIISLEAVSMTTDITEAPSKQGIFVDIAGTAVVKVDNN
                     PEKVLIAVEQFCSGNADRTTQNIKTVVEQILEGKLRGIVSTLTVEQINEDRVAFENSI
                     EDSITRELDNMGLRLLSYTVLKIATQGGYLENRAIPQIAQSKADADIASAERARDTEV
                     KTAAAVREGQKAKLEAEAEIAQSDRDKTIRMEQYRAEQDKIKANADVSYKLQEIENNK
                     IVADRNVELAKKEAQVVEEQLVASVKKPADAKKYETEVQAEANKIKSIKEAEARAQAL
                     KIEAQARADAKLLEAKAEAEAIRAQGEAEAEALKAKGIAEAEAKDRLAEAMEKYGEAA
                     MMSMVVERLPEIMAQIAKPMEQIDKITVIDNGSGDGGSKVSKIVSDVAANGFEVLKDL
                     TGADLGEVVRTIANKNSNNKPKTK"
     assembly_gap    2249..2282
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2283..>2987)
                     /locus_tag="PLK68_09135"
     CDS             complement(<2283..>2987)
                     /locus_tag="PLK68_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90606.1"
                     /translation="ILAFIDTTKEQDGSRGMIITPAQIYFKFGQAGIIEYQKIISLGL
                     EKHRNDSIIKAIIKLEEITYTFSNHIIDPKVFVTMLSKITGIEIEMIMDTHEKVEYYT
                     RIVLNDLENDEYEDIVLTPTQNNSIKEFYKELEMVQQLADEDYQYELENICNRALEFF
                     DVLGLDSDEIDGLLECQNQFNQKDIEEEQKIDNAQQFYNDMMNKYQQGDSEMFNRVKS
                     IMSSMGIDENDLAGKSP"
BASE COUNT         1038 a          465 c          489 g          961 t
ORIGIN      
        1 atttatataa taatttaaat tatctagtca atgatgccgt tttgcaagaa cctagttcaa
       61 acgatttgag ctatctggat aatattgaaa ataatgaaaa ttacaatgtt gctatcacat
      121 atttaaatta tgttgaagat ggcttaaatt atcttgattt aattagtgtt acaagaggta
      181 atgatgattt attagaaatt aggaatgatt ctgagtatta tttgtatatc aagtatcttg
      241 acaatggcta tacaaataat gttttaatcg ttcctaatga tacttattat tgctccatta
      301 tcgatgagga agaattaact atagagacta ttgcatttta taattttgaa gatccggcat
      361 tagatgtctt cactaattta tcagatattg atatctatat gaatgttgat agtattgatt
      421 ttttagaaac aaatgcggaa attttattta aacattatta ttatcaagct tatttaaaac
      481 tgccatatga tgacattatg gtcatcaaag aaagagattc taataaggga tattatggtt
      541 accttgatta tacaaatcgc caaatcaaag tatattactc tgataattat actcacgatg
      601 taaccattga aaatattcaa aattatcaaa gtgattatct taaaaccata acactttaga
      661 aaaggggaga aaaaatgatt tcagataatt tctttacaga acacatgact attgttattg
      721 ctgtagctat tgttctacta cttattatta ttttattgac atgctggaaa agagttcctg
      781 ctgacaaagc tttagttatc accggattaa agaaacgtgt tctaacgggt aaagggggta
      841 ttcaagtacc atttcttgaa acatcttgta tcatttcatt agaagctgtt tcaatgacca
      901 ctgatatcac tgaagcacct tcaaaacaag gaatttttgt tgatattgct ggtactgcag
      961 ttgttaaagt tgacaataat ccagagaaag tattaatcgc agtcgagcaa ttttgcagtg
     1021 gtaatgctga tcgtactact caaaatatta aaactgtagt tgaacaaatc ttggaaggta
     1081 aacttcgggg aattgtttct acattaaccg tagaacaaat taatgaagat cgtgtcgcat
     1141 tcgaaaactc aattgaagac agtattacac gagaattaga taacatggga ttacgtttgt
     1201 tatcatatac agtattaaaa attgctactc aagggggata tttagaaaac cgggcaatcc
     1261 ctcaaatcgc ccaatcaaaa gcagatgctg atattgctag cgctgaaaga gcaagagata
     1321 ccgaagttaa aactgctgct gcagtacgcg aggggcaaaa agctaaatta gaagctgaag
     1381 cagaaattgc tcaaagtgat cgtgataaaa caattcgaat ggaacaatat cgtgccgaac
     1441 aagataaaat caaagccaat gccgatgttt catacaagct tcaagaaatt gaaaataata
     1501 aaatcgttgc tgatcgtaat gttgaattag caaaaaaaga agcacaagta gttgaagaac
     1561 aattagtggc atctgtaaaa aaaccagctg acgctaaaaa atatgaaact gaggttcaag
     1621 ctgaagcaaa taaaataaaa tcgattaaag aagccgaagc tcgagctcaa gcattaaaaa
     1681 tcgaagctca ggcacgcgct gacgccaaat tattagaagc taaagctgaa gccgaagcta
     1741 ttcgagcaca aggggaagct gaagccgaag ctttaaaagc taagggaatt gccgaagccg
     1801 aggccaaaga ccgtttagcc gaagccatgg aaaaatatgg tgaggccgca atgatgtcaa
     1861 tggtagttga acgtctccca gaaattatgg cacaaattgc caaaccaatg gaacaaatcg
     1921 acaagattac tgttattgat aacggtagcg gtgatggcgg aagtaaagtt agtaaaattg
     1981 ttagtgatgt tgctgctaat gggtttgaag ttttaaaaga tctaactggt gccgatttag
     2041 gtgaagttgt tcgtactatc gctaataaaa acagtaataa taaacctaaa actaaataaa
     2101 cttaattatt cctctttaat gagcatttgc ttgttaaaga ggtcttttta tgaaaacagt
     2161 gaaacactgc tattaaatca gtgtttcact gtacctttat taaaccttcc tgctagtttt
     2221 tcaagcatac tttttgaaat accaaattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnggactttt ccctgctaaa tcattttcat ctattcccat tgatgacatt atactcttaa
     2341 ctcggttgaa catctcacta tcaccttgtt gatacttatt catcatatca ttataaaact
     2401 gctgagcatt atctatcttt tgttcttctt caatatcttt ttgattaaat tgattttgac
     2461 attctaataa accatcaatt tcatcactat ctaaccctaa tacatcaaaa aattcaagtg
     2521 ctcgattaca aatattttct aattcatatt gataatcttc atcagctaat tgctgaacca
     2581 tctctaattc cttataaaac tctttgatac tattgttttg agttggagtt aatacaatat
     2641 cttcatattc atcattttca agatcattta atacaatcct cgtataatac tctacttttt
     2701 catgagtatc catgatcatc tctatttcaa taccagtaat ttttgataac attgtaacga
     2761 atactttagg gtcgattata tggttactaa aagtatatgt tatctcctct aatttgataa
     2821 tagctttgat tattgaatca ttacgatgtt tttccaatcc taaagaaata atcttttgat
     2881 attcaattat cccagcctga ccaaacttaa aatatatttg tgctggagtg atgatcattc
     2941 ctcggctgcc atcttgttct ttggtagtat caataaatgc aaggatt
//
LOCUS       DAOMTA010000331         2981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1383_length_2981_cov_2.262474, whole genome shotgun sequence.
ACCESSION   DAOMTA010000331 DAOMTA010000000
VERSION     DAOMTA010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1383_length_2981_cov_2.262474"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLK68_09140"
     CDS             <1..239
                     /locus_tag="PLK68_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRM90607.1"
                     /translation="SAIVTSIAKDLAITNNLKYFGLYYRKTNPILPMKPTFDDDLTKQ
                     KLELADCYSSQKKVCHNLGHMFPYENWISYDEWH"
     gene            complement(249..1106)
                     /locus_tag="PLK68_09145"
     CDS             complement(249..1106)
                     /locus_tag="PLK68_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90608.1"
                     /translation="MNILMKLKNSKVLSNNEKIIADYILEHPDRILKMTAKDLSTVCY
                     VSTATIYRLCDKLELSGFADLKLKITSSLEEYLKSTENFDFNFPVKQFQTHYEIIHKL
                     KEDYEQTLSSTANLFSLDQLKRVVSTMKKAQVIDVYTSAGNIYFAQNFQFQMQEIGVS
                     INVPIDEYQQRLQAASSDNSHLAIIVSYSGRGIISDILFRILKERKTPIILISSYNYP
                     IKEVKPDHHLYISSGENHYKKISSFSTRLSILYIFDILYTCYFELNYEENLERKLDYY
                     RLIDKTNIR"
     gene            complement(1899..2543)
                     /locus_tag="PLK68_09150"
     CDS             complement(1899..2543)
                     /locus_tag="PLK68_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90609.1"
                     /translation="MDEFLSELYVEYFKKWILLQTSKDYKLYFNDNDSNIILIEAENS
                     LSRVTFNRFNIIELCVMNNNDNKLEFYIHFQMQNLGHAKRLFEEMIECIYNTIKQPVL
                     KILLSCSSGLTTSYFAEKLSQTAELLELNYQFQAVGWEKVLAAALDFDVLLLAPQISY
                     QCARIQKILPNKLVLKIPTLIFASYDCLKLFEFIKESLLLDKVNNNKTIIKLSP"
     gene            complement(2566..2868)
                     /locus_tag="PLK68_09155"
     CDS             complement(2566..2868)
                     /locus_tag="PLK68_09155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM90610.1"
                     /translation="MKILLVCNAGMSTSILVREMEKAAKEQQLELEVTAMGFTQAEKV
                     LLDWDIVMLGPQVRHQLTGLQKAAEGKVPVEVINMRDYGMMNGANVLKRALEIINE"
BASE COUNT         1039 a          501 c          355 g         1086 t
ORIGIN      
        1 caagtgcaat tgttacaagt attgctaaag atctagcaat tactaataat ttaaaatatt
       61 ttggtcttta ttatcgaaaa actaacccta tcttgccaat gaaaccaacc tttgatgacg
      121 atcttaccaa acaaaaatta gaattagcag attgttactc ctctcaaaag aaagtctgcc
      181 ataacttagg acatatgttc ccatatgaaa actggatcag ctacgatgag tggcattgat
      241 ccagtttttt atctaatatt tgttttatca atcaaacgat agtaatctaa ctttcgttca
      301 agattctctt cataatttaa ttcaaagtaa caggtatata aaatatcaaa aatatataaa
      361 attgataatc tagttgaaaa agatgaaatt tttttataat gattttcacc agagctaata
      421 tataggtgat gatctggttt cacttctttt ataggataat tatatgagga aattaaaata
      481 attggtgttt tccgttcctt caagattcta aataagatat ctgaaataat tcctctaccg
      541 ctatatgaaa caataatcgc tagatgtgag ttatcactag aagcagcctg aagtcgttgt
      601 tgatattcat caatcggcac atttatactc acaccaattt cctgcatttg aaattgaaag
      661 ttttgtgcaa agtaaatatt tccagctgat gtatatacat caatgacttg agcctttttc
      721 attgttgaaa cgactcgctt aagctggtct aaactaaata agttagcagt agaacttaag
      781 gtttgttcat aatcttcttt tagcttatga ataatttcat aatgtgtctg aaattgctta
      841 acaggaaaat taaagtcaaa attctctgta ctttttaaat actcttctaa agaactagtg
      901 atttttagct tcagatcagc aaaccctgat aattcaagct tatcacataa gcgataaatc
      961 gttgctgttg agacataaca aactgtgctt aaatcttttg cagtcatttt taaaatacga
     1021 tctggatgct ctaaaatata gtctgcaatg attttttcat tattcgatag aactttacta
     1081 ttctttaatt tcattaatat attcatcttt attcaccact tttattttac aataaacaat
     1141 accaaaaaac agtattattt atgttttaat atgatgctaa aaaactaaat ttaatactta
     1201 gtttctttta ttatcactat ttttaattct taaagctaat aattctaata aaacatattc
     1261 ttaagtttgt tcaactttaa tgattttaaa attaaggtac tgtagctctt gaccaaatga
     1321 ttttattacc tgtttttaat agttcataat taagcaacaa gtcattataa attacaatta
     1381 ctggtgatga aagaatactc atgatagctt gtatcaataa agctaagcgt tttttgtgtt
     1441 ccttcaggaa tacgacgcaa gtggtaatga taaaaatttt acttcaccag caaagttatt
     1501 acaacctgtt aataactgtc cctattccca catttaaacc ctgtagctaa aataaaaata
     1561 ctaatgatta atattttaca atatgtttat tgtatttgca ttttaaaatt tgtttaaagt
     1621 ttataccact tatccccctc aataaaggat gaatagacat agccattatc aaacactcca
     1681 aatatagaaa tcgctataca atcaatttct aaaaaatgta acagtactat atttaaaaca
     1741 tcataaatac cttgaattat aatactatcc ttaataatcc gcttttcata aatcatcaat
     1801 aactctttcc catatagatg gtaatcaata aaattcttat tattatcctg atatactaat
     1861 aaatataagg taagattatt agtgttttta ttaataattt acggtgataa ttttattatt
     1921 gttttattgt tatttacctt atctaataat agtgattctt tgataaactc aaataatttt
     1981 aaacaatcat agcttgcaaa tattagcgtt ggaattttta aaactaattt attaggtagt
     2041 attttttgta tccttgcaca ttggtaagat atttgtggag ctaagagcag aacatcaaaa
     2101 tccaatgctg ctgccaagac cttttcccag ccaactgctt gaaattgata attcaattct
     2161 aataattctg ccgtttggct aagtttttca gcaaaataac tagtcgtcaa accgctagag
     2221 catgataata aaatttttaa aacaggttgt ttgatcgtgt tataaataca ttctatcatc
     2281 tcttcaaaaa gtcttttagc gtgtcctaaa ttttgcattt gaaaatgaat ataaaattct
     2341 aatttattgt cattattatt catgacacaa agctcaataa tattaaaacg attaaaggtt
     2401 acacgactta aactattttc agcttcaata agtataatat tggaatcatt atcattgaaa
     2461 tacaatttat aatccttact agtttgtagt aagatccatt ttttaaaata ctctacataa
     2521 agctcgctta aaaattcatc cataaaatta tctcctagag acaagctatt cgttaatgat
     2581 ttcaagcgct cgttttagca cattggcacc attcatcatt ccataatcac gcatattaat
     2641 tacttctacg ggaactttac cctcagcggc tttttgcaga cctgttaact gatgcctaac
     2701 ttgtggcccc aacataacaa tatcccaatc taataaaact ttttccgcct gcgtaaaccc
     2761 cattgctgtg acttctaatt caagctgttg ttcttttgcc gctttttcca tctctcgaac
     2821 aagtatactc gttgacatac cagcattaca tactaacaat attttcatct cattcacccc
     2881 tatttattat aaaaatcatc gtacatttta agcgctgcag ctaatacttt ctctccattc
     2941 atcaatccgt aatcacgcat atcaataaca tcaaacggaa t
//
LOCUS       DAOMTA010000332         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1388_length_2977_cov_2.519849, whole genome shotgun sequence.
ACCESSION   DAOMTA010000332 DAOMTA010000000
VERSION     DAOMTA010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1388_length_2977_cov_2.519849"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="PLK68_09160"
     CDS             complement(<1..1620)
                     /locus_tag="PLK68_09160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM90611.1"
                     /translation="MKKIGKNIVNKKPSIYPVGKLIYIYIILIVIIVLMIFNTINLNN
                     TLGSSTNTYVNDVTSQLADDISIRIEAIKSSLRLLSDSINILPDNQEISSFLQKKCEN
                     LEFTDLFVIDDKGNTIPEQSETFDLSLASSSFKGENNLIYLDGQSLLFSTPIYDEKGI
                     SKVLIGIRDKENIQNLIKPKSFNGQGLSCIIDQNGNVVISPTDLKPFMQLDNIFKDGT
                     DTDAVNHIKKMRKNIINGKSGVFEFTASDDTSLIISYHYLDHNDWTLLTLVPGELISS
                     GTNQYIFYNFILVACVICVFSVLLYLIFLSYKKHKNWLEKIAFTDPLTAGLNNAGFQL
                     DAQKLVNTAPPSNYTVIFLDIKQFKLINQNYGFNKGNDVLKHIYNVIYNNISSNELVA
                     RSENDHFFICLNESNKQIIQSRLDMIIAEINSFAKNKASQLTILQGACLIENPNSEIN
                     IIQDYARLACRLNSKKSKCTYYDKEILDTMKKEQELNDLFENSIKNKDFHMFLQPKII
                     ISNNKIGGAEALVRWFHPERGTIYPSDFIPLF"
     gene            1810..2382
                     /locus_tag="PLK68_09165"
     CDS             1810..2382
                     /locus_tag="PLK68_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM90612.1"
                     /translation="MKQKQTLFNYLYNNLHDLIVSGRLPYGSKLPSISELCEFYNIGI
                     RTVKDVLHVLKEEGYISTHERKATTVVYNIHSKFKEDGLEYVLEHRQEIIDVYKTIGL
                     IMPVIFSFAAQIWDEEDLQLCSQRLKESEDKSAEERERICTRIFFELLDKSHNPLLRD
                     IFSSLEIYARPVFFVNYEKYINLIQFRIYF"
     gene            2453..>2977
                     /locus_tag="PLK68_09170"
     CDS             2453..>2977
                     /locus_tag="PLK68_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM90613.1"
                     /translation="MYDTVINVIEKTLTDLALKYPEIKEMTPNYTWSAELGRDHCYTQ
                     IARDLINKISLGIYPVGSFLPPEAKLAKMYKVSVSTIRKSLHMLNELGFGETMNVKGT
                     RVVIQDEQTAIKCMQNKQYRQDTLLYLNGVQAMVILIKKAATLAFPNITQEKIKNLQG
                     EIEDSKNLMLECLLN"
BASE COUNT         1111 a          393 c          454 g         1019 t
ORIGIN      
        1 gaaaagtgga ataaaatcag atggatatat agtacctcgc tcaggatgga accagcgaac
       61 taaagcttca gctccaccga ttttattatt agaaataata atttttggtt gtaaaaacat
      121 atgaaaatct ttgtttttta ttgaattctc aaataaatca tttaattctt gttctttttt
      181 catggtatcc aaaatttctt tatcatagta agtacatttt gatttcttac tattaaggcg
      241 gcaggccaaa cgagcataat cttgaataat attaatttct gaattaggat tttcaattaa
      301 acaagcgcct tgcaaaatag ttaattgtga tgctttgttc tttgcaaatg aattaatttc
      361 agcaattatc atatcaagtc gtgattgaat aatttgttta ttagattcat ttagacaaat
      421 aaagaaatga tcattttcac ttcgagcaac aagttcatta gagctaatgt tattgtaaat
      481 aacattataa atatgtttta aaacatcatt ccctttatta aagccataat tctgattgat
      541 caacttaaat tgtttaatat ctaaaaaaat aaccgtataa ttagatgggg gcgcggtatt
      601 tactaatttt tgcgcatcta attgaaaacc agcattattc aatcctgcag tcaatggatc
      661 agtaaaggca attttctcta accagttctt gtgttttttg taagatagaa aaattaaata
      721 taaaagaact gagaaaacac aaatgacaca agcaactaaa ataaagttat agaaaatata
      781 ttgatttgta ccacttgaaa tcagttcgcc aggaactaag gttaataatg tccaatcatt
      841 atggtcaaga taatgataag atattattaa agaagtatca tcactagcag taaactcaaa
      901 aacaccagat ttaccattaa tgatattttt acgcattttt ttaatatgat ttacagcatc
      961 agtatctgtt ccatctttaa agatattatc taactgcata aatggtttaa gatcagtggg
     1021 ggaaatgaca acattaccgt tctgatcaat aatgcaagat agaccttggc cattaaaact
     1081 tttaggttta attaaatttt ggatattttc tttatcacga ataccaataa gtacttttga
     1141 tattcctttc tcatcataga tcggtgtaga aaaaagaaga ctctgaccat caagatagat
     1201 taggttattt tcacctttaa atgatgaact tgctagtgat aaatcaaatg tttcagattg
     1261 ctcgggaatt gtattgcctt tatcatcaat aacaaacaaa tcagtaaatt ctaaattttc
     1321 acattttttt tgaagaaaag aagaaatctc ttgattatca ggcaaaatat ttattgaatc
     1381 agataatagt cttaacgaag attttatagc ttcgattcgt atagaaatat catctgctaa
     1441 ttgtgaagta acatcgttta cataagtatt ggtacttgag cccaaagtat tatttaaatt
     1501 aattgtatta aaaatcatca agacaattat gacgattaaa attatataaa tatatattaa
     1561 tttaccaaca gggtagatag aaggtttttt attaactata tttttaccaa tttttttcat
     1621 tataaaaaac tcccaactat aaaattatat caatttaatt ttatcattac cgtaaaaata
     1681 aacaattaaa taattgggta cataagcata tagtagaaag attttttttg tgataaaatt
     1741 atatttatat aaataaatct aagtaattta atattatgtg cctatttaac gataaaggag
     1801 ttgatcgtag tgaagcaaaa acaaacgtta tttaattatt tatacaacaa tcttcatgat
     1861 ttgattgtaa gtggacgtct tccttatggc tcgaagctac catcaatttc tgagttgtgc
     1921 gaattttaca atattggaat tcgaacggtt aaagatgtat tgcacgtatt aaaagaagag
     1981 ggctatattt cgacccatga acgtaaagcg acaaccgttg tgtataacat tcatagtaaa
     2041 ttcaaagaag atggccttga atatgtatta gagcatcgtc aggaaataat cgatgtgtat
     2101 aaaacgattg gtttaattat gccggtaatt ttttcttttg cagcacagat atgggatgaa
     2161 gaagacttac aattatgctc tcaaagatta aaagaatcag aagataagag tgcagaggaa
     2221 cgtgaacgga tatgtactcg aatatttttt gaactgttag ataaatcaca taatccttta
     2281 ttacgagata ttttctccag cttagaaata tatgcaagac cagtattctt tgttaattat
     2341 gaaaaatata ttaatttaat tcaatttaga atatactttt aaaagtatta catgggttac
     2401 aagttcgtta ttaactaggg ataaaagtga aattgaatat cgttttggtt taatgtacga
     2461 tacagttatc aatgttattg aaaaaacatt aacggaccta gctcttaaat atcctgaaat
     2521 aaaagaaatg acacccaact atacctggtc tgcagaatta ggcagagatc attgttatac
     2581 acaaatagct cgtgatttaa ttaataagat ctcattaggt atttatccag tgggaagctt
     2641 tttaccgcca gaagcaaaat tagctaaaat gtataaagtt agcgtttcta caattagaaa
     2701 aagtctacat atgttgaatg aattaggttt tggtgaaaca atgaatgtta aaggtactag
     2761 ggtagtgata caagatgagc agactgcaat taaatgtatg caaaataaac aataccggca
     2821 agatacttta ttatatttaa acggtgttca ggcaatggta attttaatca aaaaagctgc
     2881 aaccttagct tttccaaata ttacgcaaga aaagatcaaa aatcttcagg gtgaaataga
     2941 agattcaaag aatcttatgt tggaatgttt gcttaat
//
LOCUS       DAOMTA010000333         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1396_length_2971_cov_2.246914, whole genome shotgun sequence.
ACCESSION   DAOMTA010000333 DAOMTA010000000
VERSION     DAOMTA010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_2971_cov_2.246914"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1193
                     /locus_tag="PLK68_09175"
     CDS             204..1193
                     /locus_tag="PLK68_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRM90614.1"
                     /translation="MALSKEIGIDLGTANILIYVKGEGIVVNEPSVVAIDDETKKPLA
                     VGQEAKDMLGKTPGRVKAIRPLKDGVIADFRITEILITHFINKLNLKGIFSRPTILIC
                     CPSNITSIEKSAIKDVAERCGAKRVFIEEEPKVAAVGAGLEISKPTGNMVIDIGGGTT
                     DIAVLSLGDIVTSSSLKVAGDVMDEDIIKFVKDKYKLLIGDRTAEQIKMEIGIAIKGI
                     SEETFEVRGRDLVTGLPHTITLTADETELALRETCQTIVRETKHVLEQTPPELAADIM
                     SRGIFLTGGGALLTGLDHLLENELGVPVFVADDALNCVANGCGVMLENTHYVK"
     gene            1362..1538
                     /locus_tag="PLK68_09180"
     CDS             1362..1538
                     /locus_tag="PLK68_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90615.1"
                     /translation="MVHRVKRVSIVVLSMLVLLSTLIVGTYAKATNLVSNGDFSQGTN
                     YWSMNQSDGSLAMM"
     gene            1623..>2971
                     /locus_tag="PLK68_09185"
     CDS             1623..>2971
                     /locus_tag="PLK68_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRM90616.1"
                     /translation="MALSANKKYQLSFDVEASMSGDISLDIESTSDYNKKYLDRQMIA
                     VSNQKKTYTFDFEKKDDDAVTLVYHLAKGDGISDFSNQIIKLSNVTIIEIGDIEIQKA
                     GDWYLNEGDGANGKLSGEPNDLKVKVTSINQENYWWALSLKKENLVLSGNKIYKIVFD
                     AKASKAGTIGIDIEKSADYNVKYLDKQNFDLTTTSKEYSFNFIKKDNDDVSIVFLLNE
                     GDGISNFKDETINIRNVRIEEVNDLGDEYVVNGDFETNTTSGWNTYNSNGSNLKINAV
                     NQQLEVTFPNSNGSNYWDCQLFQGDILLDNGIYRLSYDLKGSKAGTIYFDVEDTGDYA
                     TKYYPETMVDFTKDVQTYNFEFEINADNLLGTQKNAKIQFNLGPNEHCDSLAGETLYF
                     DNIKIEKIGSGAGTGELQTTNVTFDGNDVLVNNFKGLGVQWDPYVVHPLTDEEWQTVT
                     "
BASE COUNT         1093 a          337 c          598 g          943 t
ORIGIN      
        1 attaaagaac cacttacact ttattttaga gaaggacaat caagcattaa accccgaaaa
       61 atattttaat caagaggtct caaaaatagc ggtataatca accaaatagt gataattaaa
      121 aaaattatcg ctatttggtt ttttgccttg taaatatcta aattcatggt acaatagtta
      181 agaaaaaata aggaggacga atgatggcat tatcaaaaga aattggtatc gatttaggta
      241 ctgcaaatat tttaatttat gtcaaaggtg aaggtattgt tgtaaatgaa ccttctgtag
      301 tagcgataga tgatgaaaca aaaaaacctt tagctgtggg tcaagaagct aaagatatgt
      361 taggaaaaac accaggaaga gtaaaagcaa tccgtccatt aaaagatgga gttattgctg
      421 attttagaat tactgaaatc ttaattactc attttattaa taaattaaac ttgaaaggga
      481 ttttttcaag accaacgatc ttaatttgtt gtccatcaaa tattacatca attgaaaaaa
      541 gtgcaatcaa agatgtggct gaaagatgtg gggctaaacg tgtttttatt gaagaagaac
      601 caaaagtagc tgcagttgga gcagggcttg aaatctctaa accaacagga aatatggtaa
      661 tcgatatcgg tgggggaact actgacatcg cagtattatc attaggtgat attgttacta
      721 gcagttcttt aaaagttgct ggtgatgtaa tggatgaaga tattattaaa tttgttaaag
      781 ataaatataa gttattgatt ggtgatcgca ctgctgaaca aatcaaaatg gaaattggaa
      841 ttgcaatcaa aggaatcagc gaagaaacat ttgaagttag aggacgtgac ttagttacag
      901 gattacctca tacaatcact cttacagctg atgaaacaga attagcatta cgtgaaactt
      961 gccaaacaat cgttagagaa actaagcatg tcttagaaca aacacctcca gaattagctg
     1021 ctgatattat gtcacgtggt atcttcttaa caggtggagg cgctttatta acaggattag
     1081 atcatttatt agaaaatgag cttggggtac cagtatttgt agctgatgac gctttaaact
     1141 gtgtagcaaa tggttgtggt gtcatgttag aaaatactca ctatgtaaaa taattttaag
     1201 gatatggaaa catatccttt ttttgtactg aaaagtcatt tcaagaaata cgtttcatag
     1261 atatgaaatg ctgaaacgca gtacctaaat gactttggaa gattgtttta agtttattta
     1321 ttgttaaaat ttgaatatag aaataaatgg agggaagagc aatggtacat agagttaaac
     1381 gggttagtat agtggtatta agtatgttag ttttgttgag tactttgatc gttgggactt
     1441 atgcaaaagc aactaatttg gtatcaaatg gtgattttag tcaaggaact aattattgga
     1501 gtatgaatca aagtgatggt tcgttagcaa tgatgtagag tgataatcat tctttaaagg
     1561 tacagattga aaaggtcaat agtgacgact attggtggtc cttaacactc aagcaggatg
     1621 atattgcctt atcagcaaat aaaaaatatc agttatcatt tgacgtagag gcatcaatga
     1681 gtggagatat tagtttggat attgaaagca caagcgatta taataaaaaa tacttagatc
     1741 gtcaaatgat agcggttagt aatcaaaaga aaacatatac atttgatttt gaaaagaaag
     1801 acgatgatgc tgtcacttta gtttatcatc ttgctaaagg cgatggaatc agtgatttta
     1861 gtaatcaaat aatcaagctt tctaatgtta caattattga aattggcgat attgaaatcc
     1921 aaaaagcagg tgattggtat ttaaatgaag gagatggagc gaacggtaaa ttgtcaggtg
     1981 agccaaatga tcttaaggtt aaagttacta gtattaatca agaaaactat tggtgggctt
     2041 tatctttaaa aaaggagaat ttagtattaa gtggaaataa gatttataaa atagtttttg
     2101 atgctaaagc ttcaaaagct ggaacaattg gtattgatat tgaaaaatca gcagattata
     2161 atgtcaaata tttagataaa cagaattttg atttaactac tacgagtaaa gagtacagtt
     2221 ttaattttat aaaaaaagat aatgatgatg tttcaattgt atttttatta aatgaaggag
     2281 atggaattag taatttcaag gatgagacta ttaatattag aaatgttcgt atagaggaag
     2341 ttaatgattt aggtgatgaa tatgttgtaa atggtgattt tgagacaaat acaacaagtg
     2401 gctggaatac gtataacagt aatggttcaa atttgaagat caatgcagta aatcaacaat
     2461 tagaagtaac ttttccaaac tcaaatggca gtaactattg ggattgtcaa ttgtttcaag
     2521 gagatattct tttagataat ggaatctatc gtttatctta tgatttaaaa ggcagtaaag
     2581 ctgggactat ttactttgat gttgaggata ctggtgatta cgcaacaaaa tattacccag
     2641 aaacaatggt tgatttcaca aaagatgttc aaacatataa ttttgagttt gagattaatg
     2701 ctgataattt attagggact caaaaaaatg ctaagatcca gtttaattta ggtcccaatg
     2761 aacattgtga cagcttggct ggagaaactt tatattttga taatatcaag atagagaaaa
     2821 ttggttctgg tgcaggaact ggtgaattac aaacgactaa tgtgactttt gatggaaatg
     2881 atgttttagt taataatttt aaaggtttag gtgttcaatg ggatccttat gttgttcacc
     2941 ctttaactga tgaagagtgg caaacagtga c
//
LOCUS       DAOMTA010000334         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1406_length_2956_cov_2.005171, whole genome shotgun sequence.
ACCESSION   DAOMTA010000334 DAOMTA010000000
VERSION     DAOMTA010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1406_length_2956_cov_2.005171"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1026)
                     /locus_tag="PLK68_09190"
     CDS             complement(172..1026)
                     /locus_tag="PLK68_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90617.1"
                     /translation="MNKIIQAKLKNSKIIFIAVICILIIGIAGLLIFNATDNSSAKLD
                     NKGNIASKISNNVKEKDKQNEKEDSKGAIEEEDKDSEDTLVISKNGLNISKKSDDSVN
                     SGKKSDDEKKNNSQKSTKSNAETNNSTQKNNSNSSNKSNTSNKSNSSSSNSTNSLNNS
                     SSSVSKPSHEHVYNIPVTQKVHHDAVTKTETVTVVDQAAYDEPIYESHQVCKVCNKDF
                     GKGENATVEAINHSVETGHSYHSTSVKVGTKHHAAITHQETKTIVVTPAYDEEKVTGY
                     RCSCGKTK"
     gene            complement(1100..1723)
                     /locus_tag="PLK68_09195"
     CDS             complement(1100..1723)
                     /locus_tag="PLK68_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90618.1"
                     /translation="MKWKIIALLLVAVLISGCGFHQENKENQKNENYYKLNTDTTISN
                     YEKLSNDGIDYIYPNFLSRKWSVSKQLERESSMKVNKSNDAEFYVIRKNYLNFLNKVD
                     KSLDGHELKSCMQEEIKKYLGSLTPEDSKMNYVNNIKFYDEYGISSKDNVKKYYYMIS
                     FQSDLEIPSVGHYKVPTLMIVKYSDKNDMNNVKEMFENMKSWMLKAE"
     gene            complement(1740..2696)
                     /locus_tag="PLK68_09200"
     CDS             complement(1740..2696)
                     /locus_tag="PLK68_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM90619.1"
                     /translation="MKKDKELIEKLKKNDLVFLASHLGFTPVSKSSKYYTLEEHDSFM
                     IKLQDNTFQWYSRGNYGNAISMCMYLGAEVDKRFQSYSYSVDYLEKLYLQMKDNIIID
                     NIKPHENVKKDPETLKLPFSANNNKKVYSYLRNRCISEEIIDYFVYNRWLYQNRYTDN
                     LVFVSYKEDKPVFVCEKGIIQEQRFMKEYSANDYNHCFYIDHGSDCLIVTEAIVDMMS
                     LMELAEDYKRYNYLSINSVTKYQAIFEHLKNDPKIKSVLMRVDHDKAGIKLNKKVCSE
                     LKEQFPHIRVSVEYPPKEKDWNDYLVYQINQREKVDAYTIIF"
     gene            complement(2696..>2956)
                     /locus_tag="PLK68_09205"
     CDS             complement(2696..>2956)
                     /locus_tag="PLK68_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90620.1"
                     /translation="IEDAVINKIWDVAKTNKNLYVDQYDYGRFGGEDVPKWKMNELEW
                     FMKLKEEVPVNVRLKYRMRFANEINDYLNSQKKLEDEKEMAL"
BASE COUNT          879 a          509 c          355 g         1213 t
ORIGIN      
        1 ttaataaaaa taaaagacaa tccgaagatt gccttaaggt ttgaacaccc atttttccat
       61 atatattttt gtaatcattt aatttccttt ctcttctttt ttcaaaaata aaaaggacat
      121 atcttaaata tcttatgccc ttgattgatt tactgaattt ttttaatgaa actatttagt
      181 tttaccacac gaacatctgt agcctgtcac tttttcttcg tcgtaagcag gtgtcacaac
      241 aattgtcttt gtttcctgat gtgtaattgc agcgtgatgt tttgtcccca ctttaacact
      301 tgtagaatga taggaatgtc ctgtttctac actatgattg atagcttcaa ctgtagcatt
      361 ttcaccctta ccaaaatcct tattgcatac tttacaaacc tgatgtgatt cgtaaattgg
      421 ttcatcatat gctgcctgat ctacgaccgt aactgtttct gtttttgtca ctgcatcatg
      481 atgaaccttt tgagtgacag gaatattgta aacatgttca tgtgatggtt ttgaaacaga
      541 actactcgaa ttatttaaac tatttgtact gtttgaacta gaactattcg atttattaga
      601 ggtatttgat ttatttgaac tattagaatt gttcttttga gtcgagttgt ttgtttcagc
      661 attactcttt gtactttttt ggctgttatt tttcttttca tcatcgcttt ttttgcctga
      721 attgactgaa tcatctgatt tttttgaaat atttaacccg tttttagaaa taaccaaagt
      781 atcttcactg tctttatctt cttcttcaat cgccccttta gaatcttctt tttcattctg
      841 tttatctttt tctttaacat tgtttgaaat cttacttgca atatttcctt tgttgtctaa
      901 tttagcagaa gaattatcag tagcgttaaa aataagaagt cctgctattc cgataattaa
      961 aatacaaata acagcaataa aaatgatttt actatttttt agttttgcct gaataatttt
     1021 gttcattaaa ttcatctcct ttaattcatc atatcctttt gtatttttaa acaaaaatgt
     1081 acaaaattac ataaattaat cattcagcct tgagcatcca gcttttcata ttttcaaaca
     1141 tttcttttac attgttcata tcatttttat cactgtattt aacaatcatt aacgttggta
     1201 ctttataatg acctactgat gggatttcca gatcactttg aaaactgatc atataataat
     1261 attttttaac attatccttt gaagatatac catattcatc ataaaattta atgttgttta
     1321 cataattcat tttactgtct tcaggtgtta aagaacctaa gtattttttt atttcttctt
     1381 gcatacagct ctttagttca tgtccatcta atgatttatc taccttgttt aagaaattaa
     1441 gataattttt cctaatgaca taaaactcag catcattcga tttatttact ttcatagaac
     1501 tttcacgttc taactgttta gatacactcc attttcgact caaaaaattg ggatagatat
     1561 aatctatgcc atcatttgaa agcttttcat aattgctgat tgtagtatca gtatttagct
     1621 tataataatt ctcatttttt tgattctctt tattttcttg atgaaaacca caacctgata
     1681 tcaaaactgc aaccagtaac aaagcaataa ttttccattt cattctttca ccgccttttt
     1741 taaaatatga ttgtatatgc atcaaccttt tctcgttgat ttatttgata gaccaaataa
     1801 tcattccagt ctttttcttt tggaggatat tcaacactga ccctgatatg aggaaattgt
     1861 tctttaagtt ctgaacatac ttttttattc aatttgattc ctgctttatc atgatctact
     1921 ctcatcaata cacttttgat tttaggatca ttttttaaat gttcgaaaat agcttgatat
     1981 tttgttacag agtttattga aagatagttg tatcttttat aatcttctgc aagttccata
     2041 agagacatca tatctactat ggcttcggta acaattaaac aatctgaacc atgatcaata
     2101 taaaaacaat gattgtagtc attggcactg tattctttca tgaatctctg ctcctgaata
     2161 atcccttttt cgcatacaaa aacaggctta tcctctttat aggaaacaaa tacaagattg
     2221 tctgtgtacc tgttttgata aagccatctg ttatatacaa aataatctat gatttcttct
     2281 gaaatacatc tgtttcgaag ataagaatag acttttttgt tattatttgc tgaaaatggt
     2341 aatttcaaag tttctggatc tttttttaca ttttcatgag gttttatatt atcaataata
     2401 atattatcct tcatttgcag atatagtttt tcaagataat caactgaata actgtagctt
     2461 tgaaatcttt tatcgacttc agcacctaaa tacatacaca tcgaaatggc atttccataa
     2521 tttcctcttg aataccattg aaaggtatta tcctgaagct ttatcataaa agaatcatgt
     2581 tcttctaagg tataatactt tgagcttttg ctgacaggag taaatccaag gtgagaggct
     2641 agaaacacaa gatcattttt ctttagtttt tcaatcagtt ctttatcttt tttcatcaca
     2701 aagccatctc tttttcatct tccagttttt tttgactgtt taaataatcg tttatttcat
     2761 tagcaaatct cattcgatat tttaatctta catttacagg aacttcttct tttaatttca
     2821 taaaccattc aagttcgttc attttccatt taggaacatc ctcaccacca aagcgtccat
     2881 agtcatactg atccacatac aagttcttat ttgttttggc cacatcccag attttattta
     2941 ttactgcatc ttctat
//
LOCUS       DAOMTA010000335         2944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1411_length_2944_cov_3.255452, whole genome shotgun sequence.
ACCESSION   DAOMTA010000335 DAOMTA010000000
VERSION     DAOMTA010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_2944_cov_3.255452"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..431)
                     /locus_tag="PLK68_09210"
     CDS             complement(192..431)
                     /locus_tag="PLK68_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM90621.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(436..855)
                     /locus_tag="PLK68_09215"
     CDS             complement(436..855)
                     /locus_tag="PLK68_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRM90622.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            complement(1082..1423)
                     /gene="mef(H)"
                     /locus_tag="PLK68_09220"
                     /gene_synonym="mefH"
                     /pseudo
     CDS             complement(1082..1423)
                     /gene="mef(H)"
                     /locus_tag="PLK68_09220"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
     gene            complement(1414..1713)
                     /locus_tag="PLK68_09225"
                     /pseudo
     CDS             complement(1414..1713)
                     /locus_tag="PLK68_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            2218..2571
                     /locus_tag="PLK68_09230"
     CDS             2218..2571
                     /locus_tag="PLK68_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90623.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(2631..2798)
                     /locus_tag="PLK68_09235"
     CDS             complement(2631..2798)
                     /locus_tag="PLK68_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HRM90624.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT          976 a          562 c          424 g          982 t
ORIGIN      
        1 agttaatttc aaattttata tctaattttc gattatccgc ataaacacta tacattcaag
       61 cattttgaag tatagaaaaa cactcatctt accacgaatt taccacgatt gaatgagcct
      121 tgatatgacc gtaaaactac taagggagat agcacgaaac tgtgtatctc ccgatttttt
      181 tatacagttg attacttcac tccgatttca aatttcaata ttgctttcat catgtctgta
      241 tgaatttctc cttttagttc ttcatcaacc tgcataatga ttttaccact ttcagagtgg
      301 taaaaaggac gcaagcataa tttagaaata taaccgtcat aaaacttcaa aatctcttta
      361 actgccattt catctccgct tgacgctttg caaatcaatt caaaagaggg atagccataa
      421 tctttactca tttctttact tcccttcgct caaaagcatt tttaacttcc ttagtcctct
      481 agttcttcta ctgcaaatag cagaacgtga tacatggtac agttctgaaa tttttctgtc
      541 gttcattcct tgaaaataac gtaataaaat cgcactacgc tgtttttctg acagtctatg
      601 taaaactgta ccaagtgttt catcagatac ataaattgta aagtgcaaga ctttaaatga
      661 tacacaatca taaaaacagg tatcaatcgt tccacgttca acttgtttcg tttcggctat
      721 ttcacaaaac aatacttcac gtttagaatg tcttgcaagc tgtctgtttc gacttttcac
      781 tttgttctta atgacaatca gcattaaaga attgaactgt aatctgacga tatgctcgaa
      841 agaagatgtc ctcataatct cacccccttt ctgctaatgc tgaaatatat aaacggaata
      901 ccgtttatac cctttagaaa tacagaaaaa tggtaagaaa aaagcccaca caaaacatat
      961 atttgtgcga gccttaaata ctgcaatatt actctttctt ttataacact atccttttag
     1021 cagatttcaa attttctttt atatattgtt tttagcctaa catactattt aaccccttgt
     1081 attatatctc taacttttga aaaattcttt ttgaatatat tgaaatcatg attgaagcaa
     1141 tagaagctac aaacataaca acccatgttt cttgtataaa tacatcgaat aaaataccgt
     1201 aaaccatttg tccaataggt tgaccgcaca tagcaactgc taccagcgta gccattatct
     1261 ttcctactaa ttgcggtggt gtttgtgtct gaacaagagt gtaaatttga acaacaaaca
     1321 tagtagatac acccatagcc acaaagcaca tcactgtaac tacccagtag gaaactattg
     1381 caggcaattc taaaaatagc gaaattccca taagtaaaac agcagtagaa tttcacgttt
     1441 cttgtctggc aactcacgta atgcttcact caacaaatca ttttcaacgc ctactgataa
     1501 cccattgagt gtaaaaatct gaaagtcagt tgaatagtta tctgttgtcg caaactggct
     1561 aacaagataa tcgccaacat ccgaaaagga cacctcacgc tttgcaatcc ttgaaagata
     1621 aagcatataa ttctttcgct cgtcttccat agcacgttta cagatatagt caaactgatt
     1681 ttctattgtg gtctgaaaag aagatggttt catgtttctc accccctttc tgtctaggaa
     1741 aggaagtgag ccttgctcgt ttatctcctt tcactcttag tcccaatgtg aaagggggat
     1801 ttgttgcatt actgataaat aaactttgta aaaaagttct gaatagccaa aaaagcatat
     1861 aaacagattt atttctctgt ttacatgctt ctgttattct atctatatga tttataaaac
     1921 cacattggtg gacgtactta tctattgcag atagacgact ttttttgaca agaacccaat
     1981 gtaaggaaat ttattgtata tgatgtactt catggcgacg ttgacctcca acaaaccgcc
     2041 atttggaagt aatatacaat attttaacag cgtaaatagc actaccatat aacggttttt
     2101 tttattggcg tttagtagtg ctttttatta aatataaacc tataaaccat ataacacgtt
     2161 tttctatacc tgtttttaat tcagtaggaa caataaaatg tatagaggtg gtctactatg
     2221 cgtaaaaaag aagataaata tgattttaga gcctttggtt tagccattaa agaagctcga
     2281 ttgaaacgag gtttaactcg tgaacaagtg ggagcattga ttgaaattga cccacggtac
     2341 ttaactaata ttgaaaataa agggcaacac cccagcatac aagttcttta tgaccttgta
     2401 tcgttacttc atgtttccgt tgatgaattt ttcttacctg ctaataactt ggtaaaaagc
     2461 acccgacgat tacagataga gaaatacatg gatagcttta cagacaaaga actatcctta
     2521 atggaatctt tagccagcgg tatcaacgaa gcaagaaaca tcgaagacta attaaaagaa
     2581 tccatacata acggaaagag ccgataaaat gagattgtat taatctcatt ttatcggctc
     2641 tgcgtctttg cgtctggctc tgtaatcaca gttactttga actgctttat ttcaattaaa
     2701 ttttcttgtc tgcatttcgg acaatagagg gggaattttt ttaattcagt atcttccctt
     2761 atctttaatc gtgttttatt tccacataca ggacacaata tccacttgta gtttataata
     2821 actatctcct cctttacact ttaattcaaa tctttattaa aaaatatttc atcttattta
     2881 acaagaaacc atatttatat aacaacataa aatacactaa gttattttat tgaacatata
     2941 tcgt
//
LOCUS       DAOMTA010000336         2939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1416_length_2939_cov_2.256588, whole genome shotgun sequence.
ACCESSION   DAOMTA010000336 DAOMTA010000000
VERSION     DAOMTA010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_2939_cov_2.256588"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLK68_09240"
     CDS             complement(<1..400)
                     /locus_tag="PLK68_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90625.1"
                     /translation="MDKIKKDFIVIYLARNLLATFAITVFSFVYDITNYYNMLPLRAF
                     IKIFADNFYTTAYFLLLWILNYLLFEIYKIVMDALKDRNKNHGKLVIKGKEMIAYGSI
                     IPIVILVILLIIDFNQLFKLNFILLVIFMLL"
     gene            complement(393..1244)
                     /locus_tag="PLK68_09245"
     CDS             complement(393..1244)
                     /locus_tag="PLK68_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90626.1"
                     /translation="MDKKHKQHLLVTLIFTLIVTATLFFMYDDFVFQTYGEVVYYDYI
                     LKGENNQLKVENIEAYLDRQSFHLGEGRIIFKDVNLTNGAVPTVKLSLYGENQQKFDY
                     EFVVEEYHSDNLIYSIQSISKKYKEIDLDDVKSASLTIEANDQKLSEVDLKITPVEQL
                     EGSNKEYRIENASISNSMMRLGTLKAASDDVIKEYPTVSLEYRYLKDKNGDKEDNDNY
                     VVFKKITGKSKELVNGNDYGTYNLEDEDDSFKDKDLSVVIIFSNGKEKFAFAIDLKTR
                     EVGDYYG"
     gene            complement(1511..>2022)
                     /locus_tag="PLK68_09250"
     CDS             complement(1511..>2022)
                     /locus_tag="PLK68_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM90627.1"
                     /translation="DGTDAIMLSGESAQGKYPEEAVMTMTKIALKTEETLDYASLLRK
                     AIRTAPEDPSEAICMSVAEIASKFKVSAIVVYTESGSTAKRVSRYRPESMVIAATPYE
                     PVTRSLALNWGVKGVVCQPMHDRAAQLEYAQVLAKENGVEPGEQILITAGTPGVGGTT
                     SYLELVTVK"
     assembly_gap    2023..2053
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2054..2934)
                     /gene="pyk"
                     /locus_tag="PLK68_09255"
     CDS             complement(<2054..2934)
                     /gene="pyk"
                     /locus_tag="PLK68_09255"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM90628.1"
                     /translation="MVFELKERKKKTRVVCTIGPASENEKMLRKLILAGMNVMRLNFS
                     HGDFEEHGGRIVTVRKLSKELNKNIAILLDTKGPEIRTGDFVGGKTEFKKGQTTVITT
                     EDIEGTSDRFTITYKELYKDVKPGGFILVNDGQVELLVDHVEGEDIVCVCANDGVVKN
                     KRGINVPGIKLGFDYLSPKDTADITFGCEQGVNFIAASFVRRAQDVLDVKKLLIENGH
                     PEIQIIAKIENSEGVENMDEILKVADGIMVARGDLGVEVPAEDVPLIQKQLIKKCRAA
                     GKVVITATQMLDSMQENP"
BASE COUNT          984 a          543 c          316 g         1065 t
ORIGIN      
        1 ttaataacat aaaaataacc aataaaataa agtttaactt aaataattga ttaaaatcaa
       61 ttatcaataa aattaccaga attacaattg gaatgatact cccataagca atcatttctt
      121 tccctttaat aacaagttta ccatgatttt tattacgatc ttttagtgca tccattacaa
      181 ttttatatat ttcaaataac agataattca agatccatag taataaaaaa tatgcagtag
      241 tataaaaatt atcagcaaat atcttgataa atgctcttaa cggcagcatg ttataataat
      301 ttgttatatc ataaacaaaa ctaaatactg tgattgcaaa agtagctaat aaattacgag
      361 ctagataaat aacaataaaa tccttcttta tcttatccat aatagtcacc tacttctcta
      421 gtttttaaat caatcgcaaa agcaaacttc tcttttccat tgctaaaaat aatcactaca
      481 cttaagtcct tatctttaaa actatcatct tcatcttcaa gattgtatgt cccataatca
      541 ttaccattga cgagttcttt acttttacca gtaatctttt taaataccac ataattatca
      601 ttatcttctt tatcaccatt tttatctttt aaataacggt attctaaaga tactgtaggg
      661 tattctttaa tcacatcatc acttgccgct tttagtgtcc ctaaacgcat catcgaatta
      721 gaaatacttg cattttcaat tcgatattct ttatttgaac cttccaattg ttcaactgga
      781 gtaattttta aatcaacttc acttaacttt tgatcatttg cttctattgt tagtgatgct
      841 gatttaacat cgtccaaatc aatttcttta tatttttttg agatactttg aattgaataa
      901 atcaaattat cactatgata ttcttctaca acaaattcat agtcaaattt ttgttgattc
      961 tctccatata atgatagttt aactgtagga acagctccgt tagttaaatt cacatcttta
     1021 aaaataattc gtccttctcc taaatgaaaa ctttgacgat ccaaatacgc ttcaatattt
     1081 tctactttta attgattatt ttcacctttt aatatgtaat cataataaac tacttcacca
     1141 tatgtttgaa atacaaaatc atcatacata aaaaataatg ttgctgtaac aataagggta
     1201 aagatcaatg taactagtaa atgctgttta tgttttttgt ccatttttat caccggtctt
     1261 attataacct attttttatg agatagaaag aaaaacaacc caagccaaat ttacatatta
     1321 attccttatt catcattttt ttatgaattt acaacttgct attactttta aaaaccagat
     1381 aagctcaaaa cagttactaa attaaaaggg atcgactctg cttttcacaa aatcaatccc
     1441 ttttaaaatc aaatatttaa attataaata cctatttcac aatatactta aaatctatat
     1501 gtatttaaaa ttattttaca gtaactaatt ctaaatagct agtagttcca ccaacacctg
     1561 gtgttccagc agtaattaaa atttgttccc ctggttcaac accattttct ttagcaagaa
     1621 cttgagcata ttctaattga gctgcacgat catgcatagg ttgacataca acacctttta
     1681 caccccagtt taaggctaat gaacgagtaa ctggttcata aggtgtagct gcaattacca
     1741 tagattctgg acgataacga gatacacgtt tagcagttga tccactttca gtataaacaa
     1801 caatagcaga taccttaaat ttagaagcaa tctcagctac tgacatacaa attgcttctg
     1861 aagggtcttc tggagcagtt ctgattgctt ttcttaataa tgaagcataa tctaaagttt
     1921 cttctgtctt taatgcaatt tttgtcattg tcataactgc ttcttcagga tatttacctt
     1981 gagcagactc accagataac atgattgcat cagtaccatc atnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnggatttt cttgcatact atctaacatt tgagtagcag taataacaac
     2101 tttaccagca gcacgacatt ttttaattaa ttgtttttga attaaaggta catcttcagc
     2161 aggtacttca actcctaaat ctccacgagc aaccatgata ccatcagcta cttttaagat
     2221 ttcgtccatg ttttcaacac cttcgctgtt ttcgatttta gcgataattt gaatttcagg
     2281 atgaccattt tcaattaata attttttaac atctaaaaca tcttgcgccc gtcttacgaa
     2341 agatgcagca ataaagttta ctccttgttc gcatccaaaa gtaatatcag ctgtatcttt
     2401 aggagataaa taatcaaatc ctaatttaat tcctggaaca ttgattccac gtttgttttt
     2461 aactacacca tcgttagcac atacacaaac aatatcttct ccttcaacat gatcaactaa
     2521 taattcaact tgaccatcat ttactaaaat gaaaccacca ggtttaacat ctttatataa
     2581 ttctttataa gtgattgtaa aacgatcact tgtaccttca atatcttcag tagtaataac
     2641 agtagtttgt ccttttttga attctgtttt tccacctacg aaatcaccag ttctaatttc
     2701 tggtcctttt gtatctaata agatagcaat gtttttattt aactctttgc ttaacttacg
     2761 tacagtaaca attcttccac catgttcttc gaaatcacca tgagagaagt ttaatctcat
     2821 aacgttcata ccagcaagaa ttaactttct taacattttt tcgttttcac ttgcaggccc
     2881 aattgtacaa acgacacgag ttttcttttt tctttctttt agttcaaata ccatatcct
//
LOCUS       DAOMTA010000337         2939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1417_length_2939_cov_2.205617, whole genome shotgun sequence.
ACCESSION   DAOMTA010000337 DAOMTA010000000
VERSION     DAOMTA010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_2939_cov_2.205617"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLK68_09260"
     CDS             complement(<1..256)
                     /locus_tag="PLK68_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HRM90629.1"
                     /translation="MKNDDLEVVVSNYGCTIVKVLMKDKDGNIDDVVLGYDDFRDYQT
                     KDAYLGALVGRTANRIGKGKFTLNSETYTLPINNGPNCLHG"
     gene            complement(262..1779)
                     /locus_tag="PLK68_09265"
     CDS             complement(262..1779)
                     /locus_tag="PLK68_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRM90630.1"
                     /translation="MNHEINRLIQFALDKEMITKDECDYSVNLLLDLFDENDFEYEEI
                     NEHLPLATPILEKMLDHAVTKGLIEDNTTSRDLFDTKIMNCIMPRPHTVNEKFKQKYM
                     VSPKVATDYYYKLSIASNYIRKSRTDKNIIWKKYVKYGNIEISINLSKPEKDPKEIAK
                     AKLIKSSGYPKCLLCKENVGFSGDLNRAARQTHRIIPIELATGNYYLQYSPYVYYNEH
                     CIVFNEEHKPMVINENTFKNLFSFLDIFPHYMIGSNADLPIVGGSILSHDHYQGGNYE
                     FPIQGAEVLKTLISDKYPSTLIEVVKWPLSTVRLTSENKEELVALSVKMLDYWRHYNA
                     PVLDIISHTGDIPHNTITPIARRKGDKYQIDLVLRNNRTSTKYPDGIFHPHQESHHIK
                     KENIGLIEVMGLAILPARLKDELKLLSDCLLKKAKIEEYPELDKHHSWYQQLLAQHEF
                     TETNIDEILKEAVAIKFVNVLEDAGVFKMDDYGIDALTSFVETVLKSKRLMIKLI"
     gene            1939..2913
                     /gene="galE"
                     /locus_tag="PLK68_09270"
     CDS             1939..2913
                     /gene="galE"
                     /locus_tag="PLK68_09270"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRM90631.1"
                     /translation="MSILVLGGAGYIGSHTVYQLIENGKEVVIVDNLQTGFKELIHPK
                     AKFYQGDLRDKTFLNNVFEQEKIDGVIHFAANSLVGVSMKEPLEYYDNNVYGMIVLLE
                     VMKNHSVKHIVFSSTAATYGEPKRIPIEEDDETYPTNPYGETKLAMEKLMKWCSSAYG
                     MSFVALRYFNACGAHPNGKIGELHNPETHLIPLILQVPLGIRESIYVFGDDYDTKDGT
                     CIRDYIHVMDLADAHIKALNYLKAGNPSNIFNLGNGEGYSVLEIINAAKKVTNLPIAV
                     TKAARRAGDPAKLVANNTKAKEILGWEPKYTDIEKIISTAWNFYIGRK"
BASE COUNT          988 a          486 c          421 g         1044 t
ORIGIN      
        1 caccatgtaa acaattaggg ccattattaa ttggcagcgt ataagtttca ctgtttaaag
       61 taaacttacc tttaccgata cgattagctg ttctcccaac taatgcccct aaataagcgt
      121 ctttagtctg ataatctcta aaatcatcat accctagcac tacatcatca atatttccat
      181 ctttatcttt cattaaaact ttaacaatcg tacaaccata gtttgaaaca acaacttcta
      241 aatcatcatt tttcatttga attagatcaa ttttatcatc aatcttttcg atttcaaaac
      301 tgtttccacg aaacttgtta aggcatcaat tccataatca tccattttaa aaacaccagc
      361 atcttctaat acattaacaa atttaattgc tactgcttcc ttcaagattt catcaatatt
      421 tgtctctgta aattcatgtt gcgctaacaa ctgttgatac catgaatggt gtttatctaa
      481 ttctggatat tcttcaatct ttgctttttt caataaacag tcacttaata attttaattc
      541 atcttttaat cgagctggta aaatagctaa ccccattact tcgatcagac caatattttc
      601 ttttttaata tgatgtgatt cttgatgagg gtgaaaaatt ccatcaggat attttgtact
      661 tgtccgatta tttcttaaga ctaaatcaat ttgatactta tcacctttac gacgagcaat
      721 cggtgtaatt gtgttatgcg gaatatcccc tgtatgcgag attatatcta aaactggagc
      781 attataatgg cgccagtaat ctaacatttt cactgataaa gcgactaatt cttctttatt
      841 ctctgaagtt aatcgaactg tcgataatgg ccatttaacc acttcaatca atgtacttgg
      901 atatttatca gaaattagtg tctttaaaac ttctgcccct tgaattggaa attcataatt
      961 tcccccttga tagtgatcat gacttaaaat tgatccacca acaattggta aatcagcatt
     1021 tgatccaatc atgtaatgag gaaatatatc taaaaatgaa aacagattct taaacgtgtt
     1081 ctcattaatc accatcggct tatgttcctc attaaacaca atacaatgct cattataata
     1141 aacatatggt gaatattgta aataataatt tccagtcgcc aattcaattg gaataattcg
     1201 atgagtttgt cgagccgccc gattcaaatc gccactaaaa ccaacgtttt ctttacacaa
     1261 taaacattta ggatatccgc ttgatttaat caatttagct ttagcaattt cttttggatc
     1321 tttttccggc tttgataaat tgatagaaat ctcaatattt ccatatttaa catacttttt
     1381 ccaaataata tttttatccg tacgactctt tctaatataa ttagaagcaa ttgataattt
     1441 gtaataataa tcagttgcta ctttaggact taccatatat ttctgcttaa atttttcatt
     1501 tacagtatgg ggtctaggca taatacaatt cattatttta gtatcaaata gatctctact
     1561 tgtggtatta tcctcaatta atccttttgt tactgcatga tccaacatct tttctaagat
     1621 aggtgtcgct aacggtaagt gttcattaat ctcttcatat tcaaagtcat tttcatcaaa
     1681 taaatctaat aataggttta ctgaataatc acattcatct ttagtaatca tctctttgtc
     1741 taaagcaaac tgaattaacc gattaatttc atggttcatt atagtaattc ctccctttta
     1801 cacaattata ttgtagctct aactataaat attgtcaatc atttttacta aattataagt
     1861 aaatatttac taatttattg actaatgttt actaaagaat tataatagcg ttgtaaaaat
     1921 aaaaaagggg gactcaaaat gagtatatta gtattaggtg gtgctggcta tattggaagc
     1981 catactgtat atcaattaat cgaaaatgga aaagaggtag ttatcgttga taaccttcaa
     2041 actggtttta aagaattgat tcatcctaaa gccaagtttt atcaaggaga tctacgtgat
     2101 aaaacatttt taaataatgt ttttgaacaa gaaaaaattg atggtgtaat tcattttgcc
     2161 gccaattcat tagttggtgt tagtatgaaa gagccattag aatattatga taataatgtt
     2221 tatggcatga ttgttttact tgaagtaatg aaaaatcata gtgtaaaaca tattgtcttt
     2281 tcttctactg ctgcaactta tggtgaacct aaacgtattc caatcgagga agacgatgaa
     2341 acatatccaa ctaatccata cggagaaact aaattagcaa tggaaaaatt gatgaaatgg
     2401 tgttctagcg cttatggaat gagttttgta gctttaagat attttaatgc ttgcggtgcc
     2461 catccaaatg gtaaaattgg tgaacttcat aatcctgaga cacacttgat tccattaatt
     2521 ttacaagtcc cattaggaat tagagaaagt atttatgtat ttggtgatga ttatgatact
     2581 aaagatggta cctgtattcg tgattatatt catgtaatgg atcttgccga tgcgcatatc
     2641 aaggctttaa attatttaaa agctggaaat ccaagcaata tatttaactt aggtaatggt
     2701 gagggctatt cagttttaga aattatcaac gctgccaaaa aagttacaaa tctgcctatt
     2761 gcagttacaa aggcggcacg tcgtgctggt gatcctgcaa aattagttgc taataataca
     2821 aaggcaaaag aaatattagg ttgggaacct aaatatactg acatagaaaa aataatttct
     2881 accgcttgga acttttatat tggccgaaaa taggttataa tattaagtac atcaaggag
//
LOCUS       DAOMTA010000338         2934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1423_length_2934_cov_2.315735, whole genome shotgun sequence.
ACCESSION   DAOMTA010000338 DAOMTA010000000
VERSION     DAOMTA010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_2934_cov_2.315735"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLK68_09275"
     CDS             complement(<1..370)
                     /locus_tag="PLK68_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90632.1"
                     /translation="MIVLRRLELKDIPYMLEWMHDTETKLIFQNDFLAVDEVKAENFI
                     LNSFNDDNKHFAIVDNSNDEYLGTISLKNIDNKNRNAEYAISTRRIARGKGINMLASN
                     LIINYGFNELNLNKIYLNVLS"
     gene            complement(367..1476)
                     /locus_tag="PLK68_09280"
     CDS             complement(367..1476)
                     /locus_tag="PLK68_09280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRM90633.1"
                     /translation="MNIPFARFDCMHKEIRKELDDAYNRVVNSNYFIQGKECELFEKE
                     FAEYCGANYCIGVGNGLDGLFLILKALNIKAGDEVIVPSNTYIATALAVSYVDADPIF
                     VEPEIDTYNIDTQLIEKKISKKTKAIIAVHLQGRPADMDEINKIAKKYNLYVIEDAAQ
                     AHGTMYKGKRVGTLSDASAFSFYPGKNLGALGDGGCVVTNNKDIADKVRALGNYGSDY
                     KYHHIYKGNNSRLDEMQAAFLRCKLPYLDKWNIERKKIAHRYFEGIKNPLIKLPLHEN
                     EEFDHIYHVFVIRCDKRDKLECYLNTKGIGTVKHYPIPIHLQRAYFDLNINEGALPIA
                     EEISRTVLSIPMYYGMNDDEINYVITVLNEFGEEE"
     gene            complement(1520..>2934)
                     /locus_tag="PLK68_09285"
     CDS             complement(1520..>2934)
                     /locus_tag="PLK68_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM90634.1"
                     /translation="ILGQQYLGVSGLFDQIFTILSIADLGLGTAISYSLYKPLANNDY
                     DEVSKLMSFYKKMYSYIGVVITIFGICLIPFLGHIMKGNKIDNTIIIYLLYLFNTVSV
                     YFISYKDTLILADQRNYKLTKINFLADLFINVSQLIVLVIYKNFIFYLLSKIFITLLQ
                     RVFINRFISNEYRHVDFKNKNKLNGEVLDEIKVNIKAIFFHKIGNCLVSSTDNIIISS
                     IFGMVKAGLYTNYLSLTSMVNTLLYSIFNGVTSSFGNLTVCDDKEKVENTFNILNFLG
                     FLVFGFVSICFFVLMNDFIKMWVGADYLLPIESLFIICINFYITGIRYPLDTVKEAAG
                     IYTQDRYISILHAISNLVISIFLSYVIGFNGVLLGTMISTLMVLGWNRPYTVYKYVFD
                     KSSKKYFVDYLKKVVFLLFTGIVTTAVTSVVTISQVFINLIVKGIIVLVIYSILLMVF
                     YRNNEYLRFYLKMIKKWFLV"
BASE COUNT         1048 a          501 c          271 g         1114 t
ORIGIN      
        1 tacttaatac atttaaataa atcttattga gatttaattc attaaatcca taattaataa
       61 taagattact tgccaacata ttaattcctt ttccacgagc tattcttctt gtgctaattg
      121 catattctgc attcctattc ttgttatcaa tatttttcag tgaaatagtt cctaaatatt
      181 catcgtttga attatctaca atggcaaaat gtttattatc atcattaaat gaatttaata
      241 taaaattttc agcttttact tcatcaactg ctaaaaaatc attttgaaag attaactttg
      301 tctcagtatc atgcatccac tccaacatat acggtatatc ttttaattcc aatcttctaa
      361 gtacaatcat tcttcttctc caaattcatt caagacagtt attacataat taatttcatc
      421 atcattcata ccataataca tcggaatact taatacagtt cgacttattt cttctgcaat
      481 cggtaacgct ccctcattaa tatttaagtc aaaatatgct ctttgaagat gtataggaat
      541 aggataatgt ttaactgttc caattccttt agtatttaag taacactcta atttatctct
      601 tttatcacat ctaataacaa aaacatgata tatgtgatca aattcttcat tttcatgtaa
      661 tggtaatttt attaatggat ttttaattcc ttcaaaatat ctatgagcta ttttttttct
      721 ctcaatattc catttatcta aatatggtaa tttacatcgc aagaatgcag cttgcatttc
      781 atctagtcta ctattattac ccttatatat atgatgatat ttataatctg atccatagtt
      841 acctagagct cttactttat ctgctatatc cttattatta gtgacaacac atcctccatc
      901 acctagagcc ccgagatttt ttcctggata aaaactaaat gctgaagcat cagataaagt
      961 tcccaccctt tttcctttat acatggtgcc gtgtgcttgt gcagcatcct caattacata
     1021 caaattgtat ttcttagcaa ttttattaat ttcatccatg tcagcaggtc taccttgtaa
     1081 gtgaacagca attatagctt ttgttttttt acttattttt ttctcaatta attgcgtgtc
     1141 tatattataa gtatctattt ctggttcaac aaatattgga tcagcatcaa catatgaaac
     1201 agctaatgca gtcgcaatat atgtatttga tgggacaatt acttcatctc cggctttaat
     1261 attcaatgct ttcagtatta aaaataatcc atctaaacca ttcccaacac ctatgcaata
     1321 gtttgcaccg caatattctg caaactcttt ttcaaataat tcacattctt ttccttgaat
     1381 aaaataattg ctattcacta ctctattgta agcgtcatct aattcttttc ttatttcttt
     1441 atgcatacaa tcaaaccttg caaatggtat attcataaac aatcctccct atatttttaa
     1501 tttataatat tacctacatt tatactaaaa accatttttt tatcattttt aaataaaatc
     1561 ttaaatactc attatttcta taaaatacca ttaataaaat tgaatatatc actaatacaa
     1621 taattccttt tactattaag ttaataaaaa cctgtgaaat tgttacaaca ctagttaccg
     1681 ctgttgtaac aatacctgta aataacaaaa atactacctt ttttaaataa tctacaaaat
     1741 attttttaga tgatttatca aaaacatatt tatatactgt atagggtcta ttccatccaa
     1801 gaaccattaa cgtactaatc atagttccta atagcactcc attaaaccct ataacataac
     1861 ttaaaaatat tgaaataact aaattactta ttgcgtgcaa gatagaaata tatctatctt
     1921 gcgtatatat tcctgctgcc tcttttactg tatctaatgg gtatctaatt cctgtaatat
     1981 aaaaattaat acatataata aataatgatt ctattggtaa aagatagtct gctcctaccc
     2041 acattttaat gaaatcattc attaaaacaa agaagcaaat acttacaaat ccaaaaacta
     2101 aaaagcctaa aaaattcaaa atattgaatg tattttctac tttttcttta tcatcacaaa
     2161 ccgttagatt accaaaactc gaggtgactc cattaaatat actatataac aaagtgttaa
     2221 ccatagaagt taaagataaa taattcgtat ataatcctgc ttttaccatt ccgaatatag
     2281 aagaaataat aatattatca gtagaagaaa caaggcaatt tcctatttta tgaaaaaata
     2341 ttgctttaat atttactttt atttcatcca aaacctcccc attcagttta tttttatttt
     2401 tgaaatcaac atgtctatat tcattactaa taaatctgtt aataaacact ctttgcaaga
     2461 gtgtaataaa aattttactt aataaataaa agataaaatt tttataaata accaatacaa
     2521 tcaactgact cacattaata aataagtcag ctaaaaaatt gatttttgtt aatttataat
     2581 ttctttgatc tgcgagtata agcgtatctt tgtatgatat aaaataaaca ctaactgtat
     2641 taaatagata taataagtag ataattattg tattatctat tttatttcct ttcataatat
     2701 ggcctaaaaa gggaataagg catatcccaa atattgttat aaccacacct atataggaat
     2761 acattttctt atagaagctc attaattttg atacttcatc ataatcatta tttgctaaag
     2821 gcttatataa actataacta atcgcagttc ctaatcctag atctgcaatt gataaaattg
     2881 taaatatttg atcaaataat ccacttactc ctaaatactg ttgccctaat attt
//
LOCUS       DAOMTA010000339         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1431_length_2925_cov_2.095819, whole genome shotgun sequence.
ACCESSION   DAOMTA010000339 DAOMTA010000000
VERSION     DAOMTA010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1431_length_2925_cov_2.095819"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLK68_09290"
     CDS             complement(<1..1243)
                     /locus_tag="PLK68_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90635.1"
                     /translation="MKKITAGLLAFLMLTGCSNIDNDQTLFVANNKEKYALMDYEGDK
                     QTEFIYDKYEEVGSSGYIVIKDKKYGYLLRDGEEAIKLGKYDKLESIGNMIVGYDKNE
                     KISILDGEGKELYKEDKKTEISLFGLPVIHQGKEYIVLYNDGEVLKKSKEKIISAYTV
                     DSDYAVINFEKTSSIYNLVNEKHIEGIKIGGNNQLMDHSSKKGYLLYNRKTHEINALD
                     LEGKIIFTTTLELDDLYYDNSKNIIGVKNQTTYLFNKDGETVTSNSYYRNYKNYVVKN
                     KEMIYGPHKFVNDGKEIEVNGIQLDPMASFTKSKIFPVYVRDEGYQYYGFDGKEAIKE
                     SYKSAEAFDENNLAVVSKKEDKYYLINNKGKKVSEQYVRIMYLGEKYYAGYTTGSKYE
                     VFDVEGNKVINDYFMDEGTTFV"
     gene            complement(1385..1603)
                     /locus_tag="PLK68_09295"
     CDS             complement(1385..1603)
                     /locus_tag="PLK68_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HRM90636.1"
                     /translation="MKAIVEVQYHGKNVTATDIEKLVKEDVKSQGVKISTVDTLQIYY
                     TPETSSVYYVATTKDGKSVNNEEPLVIE"
     gene            complement(1797..>2925)
                     /locus_tag="PLK68_09300"
     CDS             complement(1797..>2925)
                     /locus_tag="PLK68_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRM90637.1"
                     /translation="VNESVTCVRADGSHSQFRIQKLFGFIGLHRIEIEEAGAGDIVAI
                     AGLADIGVGETVCTTGKEEALPLLKVDEPTIQMVFGTNTSPFAGQDGKFVTARQIEER
                     LFKETNRDVSLKIERIPNSEEWIVSGRGELHLSILIENMRREGYELQVSKPKVIIKEI
                     DGVLCEPYEEVNIEAPDDCIGNVIESLGYRRGVLENMVSNEGQTSVTYTIPSRGMIGF
                     MTNFLTMTKGYGIISHSFLEYRPMEGESVGERSLGVLVSIDNGQTTAYALGGVEDRGV
                     MFIGPGVDVYEGMIVGEHSRDNDLVVNVTKGKQLTNTRSSSKDSTVVLKRPRTFNLEA
                     CLDYINDDELVEVTPENIRLRKRYLTEQARKQQNRLKQNNS"
BASE COUNT          941 a          573 c          310 g         1101 t
ORIGIN      
        1 acacaaaggt tgtcccttcg tccataaaat agtcattaat tactttattt ccttctacat
       61 caaatacttc atacttactg ccagtagtat atccagcata atatttttca cctaaataca
      121 taattcgtac atattgttca gatacttttt tgcctttatt attaattaaa taatatttat
      181 cttctttttt agaaacgaca gcaagattat tttcatcaaa agcttcagca ctcttataag
      241 attcctttat tgcttcctta ccatcaaaac cataatattg gtaaccttca tcacgcacat
      301 acaccgggaa aatcttactc tttgtaaatg atgccattgg gtctaactga atcccattta
      361 cctctatttc tttaccatca ttaacgaatt tatgtggtcc gtaaatcatt tctttattct
      421 ttacaacata atttttatag ttacgatagt aactgtttga tgttactgtt tcaccatctt
      481 tgttaaataa atatgtagtt tgatttttta ctccaataat attttttgaa ttatcataat
      541 ataaatcatc taattcaagt gttgtagtaa aaattatttt tccttctaaa tccaatgcat
      601 tgatctcatg agttttacga ttataaagca aataaccttt tttactacta tgatccatca
      661 actgattatt gccaccaatc ttgattcctt caatatgctt ttcattaact aaattgtaaa
      721 tagatgaggt cttttcaaaa ttaataaccg cataatcact atcaactgta tatgcactta
      781 taatcttctc cttactcttt tttaatacct caccatcatt atataaaaca atatattctt
      841 taccttgatg aataaccggt aacccgaata aagaaatctc tgtcttttta tcttccttgt
      901 ataattcttt tccttcacca tctaaaatac taattttttc attcttatca taaccaacaa
      961 tcatatttcc aattgattct agtttatcat attttcctaa tttgattgct tcttcaccat
     1021 cacgaagaag atatccatac tttttatcct taattacaat ataaccagaa cttccaactt
     1081 cttcatactt atcataaata aattcagtct gcttatcacc ttcataatcc ataagcgcat
     1141 atttttcttt attattagct acaaacaatg tttgatcgtt atctatattt gaacatcctg
     1201 ttagcattaa aaatgctaat aaaccagctg taattttttt catttttttc actccttcaa
     1261 aacaaaataa ttatagcatg ttttttgtcg ataaaccaca aacctttgat gataataaaa
     1321 caaaaaacga taatctaaat tatcgtttct tgctttggat atatttttgg atattatatt
     1381 aagactactc aataactaat ggttcttcgt tatttacaga tttaccatct tttgtagtcg
     1441 caacgtaata aactgatgaa gtttctggtg tataatatat ttgcaacgta tcaacagttg
     1501 aaattttaac tccctgtgat ttaacgtcct cttttaccaa tttttcaata tcagttgctg
     1561 ttacgttttt cccatgatat tgtacttcta cgattgcttt catctttaaa taattccccc
     1621 gtttcttctt gaatcaataa tatcatattc aagccaaata tgcaaatttt atagtgttaa
     1681 tcatcatttt ttaagaatta acgataaaaa tattatcttt ttttagaaaa ttcctacaaa
     1741 gtttgtctaa caaaattgaa taaaaaagaa aaaacagaaa atcgaaattt cctgctttat
     1801 gaattatttt gttttaaacg gttttgttgt ttacgtgctt gttctgttaa ataacgtttt
     1861 ctcaaacgaa tgttttcagg tgttacttca actaattcat catcattaat ataatctaag
     1921 caagcttcta agttaaatgt ccgaggtctt tttaaaacaa ctgttgagtc ttttgatgaa
     1981 gaacgggtat ttgttagttg tttacctttt gtaacattaa ctactaaatc attgtcacga
     2041 ctatgttcac caacgatcat cccttcataa acatcaacac caggaccaat aaacataact
     2101 ccacgatctt caaccccacc taaagcatag gctgtagttt gaccattatc tatcgaaact
     2161 aaaacaccaa gtgatctttc tccaactgat tcaccttcca ttgggcgata ttctaagaat
     2221 gaatgagaaa taattccata accttttgtc attgttaaaa agtttgtcat aaaaccaatc
     2281 atccctcttg aaggaattgt atatgtgaca cttgtttgtc cttcattact aaccatattt
     2341 tcaagtacac ctcgacgata tcctaatgat tctataacat taccaataca gtcatcgggt
     2401 gcttcaatat taacttcttc atatggttca caaagtacac cgtcaatctc tttaataatt
     2461 acttttggtt tagaaacttg aagttcatat ccttcgcgac gcatattttc aattaatatc
     2521 gacaaatgca actctccccg tccagaaacg atccattctt cactgttagg aattctttca
     2581 atctttaaag atacatcacg gttagtttct ttaaataaac gttcttcaat ttgacgagct
     2641 gttacaaatt ttccatcttg acctgcaaat ggcgaagtat tagtaccaaa aaccatttga
     2701 atagttggtt catctacttt taataatggt aaagcttctt cttttcccgt agtacataca
     2761 gtctcaccaa caccgatatc agctaaacca gcaatcgcca caatatctcc agcccctgct
     2821 tcttcaattt caattcgatg caagccaata aaaccaaata atttttggat tctaaattga
     2881 ctgtgtgaac catcagcacg aacacaagtt acgctttcat taact
//
LOCUS       DAOMTA010000340         2920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1438_length_2920_cov_2.424084, whole genome shotgun sequence.
ACCESSION   DAOMTA010000340 DAOMTA010000000
VERSION     DAOMTA010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1438_length_2920_cov_2.424084"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLK68_09305"
     CDS             <1..935
                     /locus_tag="PLK68_09305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRM90638.1"
                     /translation="LMFETQMGVVEDAKDVVYLRPETAQGIFVNFKNIQRTSRKKVPF
                     GIGQIGKAFRNEITPGNFIFRMREFEQMELEFFCKPDTDLEWFDYWKSYCQKFLFDLG
                     LKEENLRFRDHEKEELSFYSKATCDVEYNFPFGWGELWGIADRTNYDLTQHQNHSKKS
                     LEYLDPTTNEKYIPYVIEPSVGADRLFLSVLCDSYEEEILEDGETRVVMKLHPSVAPF
                     KVAILPLTKKQSDKATEIYSQLAKYFNCEYDVAGQIGKRYRRQDAIGTPYCVTVDFDT
                     MEDETVTVRDRDTMEQIRLPINQLVDYISKKITF"
     gene            951..2705
                     /gene="dnaG"
                     /locus_tag="PLK68_09310"
     CDS             951..2705
                     /gene="dnaG"
                     /locus_tag="PLK68_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRM90639.1"
                     /translation="MPRLSSEKINEIRQSVDIVDVIGTYLPLEKKGRNYVAICPFHDD
                     SHPSMSISPERQIFMCFVCHHGGNVFTFLKDYLKIPYIEAVKMVANIGNVDISKYNLE
                     SRVKPVDQKLEPLYRMHDEANKIYNHYLNTKLAIQAKEYLNNRKITDEIIETFEIGYA
                     PNNHVLLKAFEKMNFNKVSMFESGLIIEASNGYDRFTDRIMFPLHDASGRVVGFSGRI
                     YKQSQNESKYMNSPESSIFIKGDTLYNYHRVGEEARQAGYIIITEGFMDVIALYKAGI
                     KNAVAIMGTALTHGHLNLLKRLSKTVYLCLDGDQAGRNATIKSIDILLSAGFIVKVVD
                     LPDNLDPDEILDKRGIEELNAVIKRPLSSLDFKMNYYYEMTNMDNYEDRKSYLETIAR
                     EISRLDDIIDQDHYIQQLEKKSGFSRNIIDQLINQNTAVRIETTEPPKIPKFQNQRLL
                     DKYIRAERDLLYYMMNDKNVAMMYEAKAGFMFNDIYRIIASYIIDYYRQEVVLEVADL
                     ISSIEDENLVQNIIEISQLGLPKLKDTKAIDDYIETIKEKTVMVKKEELTKALADTFD
                     PKQKAQILKEIIALKDKE"
     gene            2709..>2920
                     /locus_tag="PLK68_09315"
     CDS             2709..>2920
                     /locus_tag="PLK68_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRM90640.1"
                     /translation="MKKKKNVTAVSFDDLKQIVLNDAKKEDGVLTQDQIDNYLSTYDL
                     NDDLAEELLEFIGNNDIVISDDGLDD"
BASE COUNT         1092 a          353 c          537 g          938 t
ORIGIN      
        1 aattaatgtt cgaaactcaa atgggtgtag tcgaagatgc aaaagatgtg gtttatttaa
       61 gaccagaaac agctcaaggt atctttgtta actttaaaaa cattcaaaga acatcacgta
      121 aaaaagtgcc atttggaatt ggtcaaatcg ggaaagcttt tagaaatgaa attacaccag
      181 gaaacttcat ttttagaatg cgtgaatttg aacaaatgga attagaattt ttctgtaaac
      241 cagatactga tttagagtgg tttgattatt ggaaatcata ttgtcaaaag tttttatttg
      301 atttaggatt aaaagaagaa aatttaagat ttagagatca tgaaaaagag gaactttcct
      361 tctattctaa agccacttgt gatgttgaat ataatttccc atttggttgg ggtgaattat
      421 ggggaattgc tgatcgaact aattacgatt taacccaaca tcaaaatcat tctaaaaaat
      481 ctttagaata tttagatcca acaactaatg aaaaatatat tccttatgtt attgaacctt
      541 cggtaggtgc tgatcgtctt ttcttatcag tactttgtga tagttatgag gaagaaattt
      601 tagaagatgg tgaaactaga gttgtaatga aattacatcc aagtgtggca ccgtttaaag
      661 ttgctatctt gccattaacc aaaaaacaaa gtgataaagc aactgaaatt tatagtcagt
      721 tagcaaaata ttttaattgt gaatatgatg ttgctggaca aattggaaaa cgttatcgac
      781 gtcaagatgc aattggaaca ccttactgtg taactgttga ttttgataca atggaagacg
      841 agacagtaac tgtacgtgac cgtgacacaa tggaacaaat tcgtttacca attaatcaat
      901 tagttgatta tattagtaaa aagatcacat tctaataagg aggaacatta atgccgagac
      961 tatcatcaga gaaaattaat gaaattaggc aaagtgttga tattgttgat gtcattggca
     1021 cgtatcttcc cttagaaaaa aaaggccgta attatgtagc gatatgtcct ttccatgatg
     1081 acagccatcc atcgatgtca atttcaccag aacgacaaat atttatgtgt tttgtctgtc
     1141 atcatggggg aaatgttttt acttttttaa aggattattt gaaaatacca tatattgaag
     1201 ccgtcaagat ggttgctaat attggtaacg ttgatataag taaatataat ttagagagtc
     1261 gagttaagcc ggtcgatcaa aagttagaac cactgtatcg tatgcatgat gaagccaata
     1321 aaatttataa tcattacttg aatactaagt tagcgattca agctaaagaa tatttaaata
     1381 atcgtaaaat tacggatgaa attattgaaa cctttgaaat tggatacgcc cctaataatc
     1441 atgtactatt aaaggcgttt gaaaaaatga attttaataa ggtttcaatg tttgagtctg
     1501 gtttgattat tgaagcttca aatggatatg atcgttttac agacaggatc atgtttccac
     1561 tacatgatgc tagtggtcgt gtcgttgggt ttagcggtcg aatctataaa caaagccaaa
     1621 atgaatctaa gtatatgaat tcgcccgagt catcaatttt tattaagggt gatactttat
     1681 ataattatca ccgcgttggc gaagaagcac gacaagcggg ttatatcatt attactgaag
     1741 gatttatgga tgttattgca ttatacaagg ctggaataaa aaatgcagta gcaattatgg
     1801 gaaccgcctt gacacatggg catttaaatt tattaaaacg tttaagtaaa accgtatatt
     1861 tatgtttaga tggtgatcag gctgggagaa atgctacaat taaaagcatt gatatattat
     1921 tgagtgctgg tttcatcgtt aaggtagttg atttaccaga taatttggat cctgatgaaa
     1981 ttttggataa gcgtggtata gaagaattaa acgctgtaat aaaaagacca ttatcttcat
     2041 tagattttaa gatgaattat tattatgaaa tgacaaacat ggataattat gaagatcgta
     2101 aatcttattt agaaacaatt gccagagaaa tcagtagatt agatgatatt attgatcaag
     2161 atcattatat ccagcaatta gaaaaaaaat caggtttttc aagaaacatt attgaccagt
     2221 taattaatca aaatacagct gtaagaattg agacaacaga gccacctaaa ataccaaaat
     2281 ttcaaaatca gcgattattg gataaatata taagagcgga acgtgattta ttatactata
     2341 tgatgaatga taaaaatgtc gcaatgatgt atgaggcaaa agcaggtttt atgtttaatg
     2401 atatttatcg aatcattgct tcttatatta ttgattatta tcgtcaagaa gttgtattag
     2461 aggttgctga tttaatcagt agtattgaag atgaaaactt ggttcaaaat ataattgaga
     2521 tatcgcaatt aggattacct aagttaaaag atactaaagc cattgatgat tatattgaaa
     2581 caattaaaga aaaaactgta atggtaaaaa aagaagaatt aacaaaagca ttggcagata
     2641 cttttgatcc taaacaaaag gcgcaaatat taaaagaaat catagcatta aaagacaagg
     2701 agtaatatat gaagaaaaag aaaaatgtaa ctgcagtatc atttgatgat ttaaagcaaa
     2761 ttgttttaaa tgatgctaaa aaagaagatg gtgtattaac acaagatcaa attgataatt
     2821 atttatctac atatgattta aatgatgatc ttgcagagga actattagaa tttattggaa
     2881 ataatgatat tgttatttct gatgatggat tagatgattt
//
LOCUS       DAOMTA010000341         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1439_length_2918_cov_1.703109, whole genome shotgun sequence.
ACCESSION   DAOMTA010000341 DAOMTA010000000
VERSION     DAOMTA010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_2918_cov_1.703109"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>563
                     /locus_tag="PLK68_09320"
     CDS             29..>563
                     /locus_tag="PLK68_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM90641.1"
                     /translation="MSKKLVIVESPSKSKTIEKYLGSDYVVTSSKGHVRDLATSGKEG
                     LGVDIENQFEPKYVINKDKKDVVKELKQLVKESDDVYLATDPDREGEAISWHLAQVLN
                     VDMDKENRVVFNEVTKDAVVDALSHPRKIDQNLVKSQETRRVLDRIIGFKLSKLLQKK
                     IKSKSAGRVQSVALRLIV"
     assembly_gap    564..574
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <575..2047
                     /locus_tag="PLK68_09325"
     CDS             <575..2047
                     /locus_tag="PLK68_09325"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRM90642.1"
                     /translation="KDKIEFSGELAKYNNKKIEIKNGEEATAIYEALNKEFEIASVKK
                     TTKRRESKPPFITSTLQQEASSKLGFKARRTMSIAQKLYEGITLENETVGLITYMRTD
                     STRLSDTFVSAAQDYIGEKYGKEYIGKVKVGKKKENVQDAHEGIRPTSALRTPESVKE
                     YLKPEELKLYSLIYARAMASLMAPAKFDATSVSLMNNGYEFKTSGSVIKFDGYLRVYG
                     DYEKQSNEILPELKEKEMLLSKNIEKTQHFTKPPARYSEAKLIKEMEELGIGRPSTYA
                     MIIDTIQTRQYVELVDKAFKPTESGILTSDRLTEYFNDIINVEYTAKMEHELDDIAEG
                     QDEYAHALQKFLDLFQPLLDNAYDKMEVIAPKKTGEKCPECGHDLVERKGRYGTFVAC
                     ENYPECKYVKKDPVEIEYTGEECPKCGSKMIFKNGRFGRFEACSNYPECKYIKNSKKK
                     EPVMTDEECPNCGSPIVIKQGRWGEFKACSNYPKCKTIIK"
     gene            2107..>2918
                     /gene="trmFO"
                     /locus_tag="PLK68_09330"
     CDS             2107..>2918
                     /gene="trmFO"
                     /locus_tag="PLK68_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRM90643.1"
                     /translation="MEKIVNVIGAGLAGVEACHQLVKRGYKVRLYEMRPKKMTPAHHS
                     GNFAELVCSNSLRADGTGNAVGVLKAEMEMMDSLIIKYARKHQVPAGGALAVDRNNFS
                     QAITEYIQSHPLIEVIHEEAKEFPAGYTIIASGPLTSDALATAIKEKLGEDYFYFFDA
                     AAPIIAKESIDFAIAYYKSRYDKGDNEYINCPMNEAQFNAFYDALVNAEVVKPKDFEE
                     KFFEGCMPFEEMARRGKQTLLFGPMKPVGLTAPDGTRPYAVVQLRQDNVQAS"
BASE COUNT         1058 a          402 c          616 g          831 t
ORIGIN      
        1 attgtgttaa tcgcgtggag gaaatgaaat gagcaaaaaa ttagttattg tcgagtcgcc
       61 atcaaaatcc aaaacaattg aaaaatattt agggagtgat tatgtagtca cttcgtcaaa
      121 aggacatgtt cgggatttgg caacttcagg aaaagaaggg ttaggtgtcg atattgaaaa
      181 tcaattcgaa cctaaatatg ttataaataa agacaaaaaa gatgttgtta aggaattaaa
      241 acaacttgta aaagaatcag atgatgttta tctagcaact gaccctgacc gcgaaggaga
      301 agcaatttca tggcatttag ctcaagtatt aaatgtcgat atggataaag aaaatcgtgt
      361 tgttttcaat gaggttacaa aggatgcagt agttgatgca ctaagtcatc ctcgtaaaat
      421 tgaccaaaat ttagttaagt ctcaagaaac aagaagggtt ttagatcgga ttattggatt
      481 caaactttca aaactgctac aaaaaaagat caaatctaaa agtgcaggtc gtgttcagtc
      541 tgttgcgtta agattgattg ttgnnnnnnn nnnnaaagac aaaattgaat ttagtggtga
      601 actagctaaa tataataata agaagattga gataaaaaat ggtgaagaag ctactgcaat
      661 ttatgaagct ttaaataaag aatttgaaat cgctagtgtc aaaaaaacga ctaaacggcg
      721 tgaatcaaaa ccacctttta ttacttcgac tttacaacaa gaagcatctt ctaaattagg
      781 ttttaaagcg agaagaacaa tgtcaattgc tcaaaagctt tatgaaggga ttactttaga
      841 aaatgagaca gtcggattaa ttacttatat gcgtactgac tcaactcgtt tatcagatac
      901 ctttgtcagt gcagctcaag attatattgg tgaaaaatat ggtaaagagt atattggaaa
      961 agtaaaagta ggaaagaaaa aagaaaatgt tcaagacgct cacgaaggga ttcgtcctac
     1021 gagtgcatta agaacgccgg aaagtgtaaa agaatattta aagccagaag agttgaaatt
     1081 gtattcatta atttacgctc gagcaatggc ttcattgatg gctccagcaa aatttgatgc
     1141 aacttcagta tcattgatga ataatggtta tgagtttaag actagtggtt cagtaatcaa
     1201 atttgatggg tacctaagag tttacggcga ttatgaaaaa caaagtaatg aaattttgcc
     1261 ggagcttaaa gaaaaagaaa tgttattaag taaaaacatt gaaaaaactc aacattttac
     1321 taaaccacca gctcgttata gtgaagcaaa attaattaaa gagatggagg aactgggaat
     1381 tggtcgtcct agtacttatg caatgattat tgatacaatt caaactcgtc aatatgttga
     1441 attggttgat aaagctttca aaccgacaga atctggaatc ttgacaagtg atcgattaac
     1501 agaatatttt aatgatatta tcaatgttga atatacagcg aaaatggaac atgagcttga
     1561 tgatattgct gaagggcaag atgaatatgc tcacgcttta caaaagttct tagatttatt
     1621 tcaaccgttg cttgataatg catatgataa aatggaagtg attgcaccga agaaaactgg
     1681 agaaaaatgt cctgaatgtg gacatgatct agttgaacgt aaaggacgtt atggaacttt
     1741 tgttgcctgc gaaaattatc cagaatgcaa atatgtaaaa aaagaccctg tagagattga
     1801 atatacgggt gaagaatgtc ctaagtgtgg aagtaagatg atttttaaaa atggtcgttt
     1861 tggacgtttt gaagcatgtt caaattatcc tgagtgtaag tatattaaaa acagcaagaa
     1921 aaaagaacct gttatgacgg atgaagagtg tccaaactgc ggttctccga ttgtaatcaa
     1981 acaaggacgt tggggtgagt tcaaagcttg ttcaaattac ccgaaatgta agacaatcat
     2041 aaagtagaac gaaagttcta cttttgtttt caaaatagaa atgataaaat tagaaaaggt
     2101 gataaaatgg aaaaaattgt aaatgtaatt ggagcaggtc tggctggtgt ggaagcctgt
     2161 catcaattag taaaaagagg atataaagtt cgactgtatg aaatgcgtcc taaaaagatg
     2221 accccagctc atcatagcgg taattttgca gaactggttt gctctaattc acttcgtgct
     2281 gatgggactg gaaatgcggt cggtgtttta aaggcagaga tggaaatgat ggatagtctc
     2341 attattaaat atgctagaaa acatcaagtc ccagcgggag gagcacttgc agttgaccgt
     2401 aataattttt ctcaagcgat aacggagtac attcagagtc atcccttgat tgaagtaatt
     2461 catgaagagg caaaagaatt tcctgcaggt tacacaatta tcgcatctgg tcctttgaca
     2521 agtgatgctc ttgcaacagc aattaaagaa aagttagggg aagattattt ttattttttt
     2581 gatgcagcag caccaatcat tgcaaaagaa agcattgatt ttgcaattgc gtattataaa
     2641 tctcgctacg ataaagggga taatgaatat attaattgtc ctatgaatga agcacaattc
     2701 aatgcttttt atgatgccct agtcaatgcg gaagttgtta aacctaaaga ttttgaagaa
     2761 aaattttttg aaggctgtat gccttttgaa gaaatggctc gccgtggaaa acaaacgttg
     2821 ttatttggac cgatgaaacc agttggactt actgcgccgg atgggacgcg cccttatgct
     2881 gttgtgcagt taagacaaga caatgtccaa gcaagttt
//
LOCUS       DAOMTA010000342         2916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1440_length_2916_cov_2.399161, whole genome shotgun sequence.
ACCESSION   DAOMTA010000342 DAOMTA010000000
VERSION     DAOMTA010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_2916_cov_2.399161"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="gpr"
                     /locus_tag="PLK68_09335"
     CDS             <1..859
                     /gene="gpr"
                     /locus_tag="PLK68_09335"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HRM90644.1"
                     /translation="KVEKVSILQAHQSINQGIGTYIEISFKDYLEQTAIIKEVVNNLK
                     PLIDKIDYPKILMVGLGNQYLTNDAIGPRTLRDVRITHFLDDEDKLINHYYDILGIAP
                     GVMVQTGMETGAIVKALVNKEDIDLVIVVDALCAKNYHKLAHVIQINDVGINPGSGIG
                     NYRQAINEETVGAKVIAIGIPTVIYASSLVSDVLNYTLDYFGDSLNPISKLKVGKRER
                     YHGSLNEQQKEMMLGQLGKLDDSELELLFNEILNPIDCNFVLSDKQIDEQCEIMSKII
                     SKSINALRY"
     gene            912..1661
                     /locus_tag="PLK68_09340"
     CDS             912..1661
                     /locus_tag="PLK68_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRM90645.1"
                     /translation="MRLKNTFLILLKLAVIVGLIVYLPTKVSGSNAQVINAINNEQNN
                     PTTPVATNPTNTSFVPTKDKKIHIYNTHQGEEYDGFNVVEGANYLKDCLNNKGYQCDV
                     EGNDFEGYKAVHKIAYNKSYTVSKMYLEESLKLSGGYDLIIDFHRDSISKELSTISHD
                     GKSYAKIMFVVGKSSGKFDAVNQLSQKLSDLANATVPKISRGVYVKKSHYNQGISDNM
                     VLIEVGAQSNTKEEVQATVEIIAKAIGEYLG"
     gene            1658..1942
                     /locus_tag="PLK68_09345"
     CDS             1658..1942
                     /locus_tag="PLK68_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90646.1"
                     /translation="MKNFLIDLCLVGLLLFMINGLFGDYGIQKAVFDKNITQFEEDVK
                     EQKPITTDYGTTNDNEENTLSLVVKQISEVCIKVIETLVLIISNIISTLL"
     gene            1965..>2916
                     /gene="fmt"
                     /locus_tag="PLK68_09350"
     CDS             1965..>2916
                     /gene="fmt"
                     /locus_tag="PLK68_09350"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRM90647.1"
                     /translation="MKDAKIIFMGTASFSKQVLEKLLENNYNVVAVVTQPDRFVGRKK
                     VLTMPEVKEVALASGIPVYQPLKIKEEYQEIIALEPDLIITAAYGQIVPEAVLNAPKI
                     GCINVHASLLPKYRGGAPVHYAIMEGEEVTGVTIMYMVKKMDAGNIISQVEVPIGAEE
                     TTGELYERLSIAGAELLLETLPSVLAGTNESIAQDESLVTYSPTISHEQEKIDFSKSA
                     LRVYNQVRGMNPWPGAYTTYQGKVVKIWAGKIHVCENALKHHAHQENGTIVKIFKDAI
                     GVKTGEGIYLITELQLAGKKRMLVKDYLNGNNIFEVDSKFE"
BASE COUNT         1046 a          349 c          596 g          925 t
ORIGIN      
        1 taaggttgaa aaagtttcga tccttcaggc tcatcaaagt attaatcagg ggattggaac
       61 ttatatcgaa ataagtttta aagactattt ggaacaaact gctattatta aggaagttgt
      121 aaataattta aagccattaa ttgataagat tgattatcct aagattttga tggtagggtt
      181 aggtaatcaa tatttaacta atgatgcgat tggcccacga actttaaggg atgtgcggat
      241 tactcatttt cttgatgatg aagacaaatt aattaatcac tattatgata ttttaggtat
      301 tgctcctggg gtgatggtgc aaacagggat ggaaactgga gcgattgtaa aagcgttagt
      361 gaataaagag gatattgatc ttgtaatcgt tgtagatgcc ctctgtgcta aaaattatca
      421 taaattagct catgtaatcc aaattaacga tgtagggata aatcctggta gtgggatagg
      481 taattatcgt caggcaatca acgaggagac tgtgggtgct aaggtaattg caattggtat
      541 accaactgtt atttatgcaa gcagtttagt aagtgatgtc ttaaattata cgctagatta
      601 ctttggtgat agtttaaatc caataagtaa attaaaagta ggaaagcgtg aacgttatca
      661 tgggagctta aatgaacagc aaaaagaaat gatgttagga caattaggaa aattggatga
      721 tagtgaatta gagctgttat ttaatgaaat attgaatccg attgattgta attttgtatt
      781 aagtgataag caaattgatg aacagtgtga aattatgtca aaaataataa gtaaaagtat
      841 taatgcgtta aggtactaaa tcctacaatt catgcatact attgtattgt atagtatagt
      901 aggtgatgca gatgagactt aaaaatacgt ttttaatttt attgaaattg gcggttattg
      961 taggtttaat tgtttatctt ccaactaaag taagcggatc aaatgcccaa gttattaatg
     1021 caattaataa tgaacaaaat aatccaacta ctccagtggc aaccaatcca accaacacta
     1081 gttttgttcc cacgaaagat aaaaagattc atatttataa tactcaccag ggtgaagaat
     1141 atgatggttt taatgtcgta gaaggagcta attatttaaa agattgttta aataataaag
     1201 gataccagtg cgatgttgaa ggaaatgatt ttgaaggcta taaagcggtt cataaaatag
     1261 cttataataa atcatatacg gtatctaaaa tgtatcttga agagagttta aagcttagtg
     1321 gtggttatga cttaattatt gattttcatc gcgattcgat ttctaaagag ctgtcaacca
     1381 ttagccatga tggaaaatca tatgctaaaa tcatgtttgt tgtaggtaaa agcagtggta
     1441 aatttgatgc ggttaatcag ctttcacaaa aactgtcaga tttagctaat gcaacagttc
     1501 caaaaatcag ccgtggtgtt tatgtgaaaa aatctcacta taatcaagga attagtgaca
     1561 atatggttct aatagaggtt ggcgctcaaa gtaatactaa agaagaagtt caagcgacag
     1621 tagaaattat tgctaaagcg attggagaat atttaggatg aaaaattttt taattgatct
     1681 ttgtttagtt ggattattgt tatttatgat taatggttta tttggagact acggaatcca
     1741 aaaagctgta tttgacaaga atattactca gtttgaagag gatgttaaag aacaaaaacc
     1801 aattactacc gattatggaa ctactaatga taatgaagaa aatacattat cattagtagt
     1861 taaacagatt agtgaagttt gtattaaagt tattgaaaca ttagtattga ttatttccaa
     1921 tattattagt acacttttat agtaagaaag tgatggtgta tattatgaaa gatgcaaaaa
     1981 taatatttat gggaacagca agtttttcta aacaggtttt agaaaaattg ttggaaaata
     2041 attataatgt tgttgctgta gtaactcagc ccgatcgttt tgttggtcgt aaaaaggtgt
     2101 taacaatgcc tgaagttaaa gaagttgctt tggcttctgg gataccagtt tatcagcctt
     2161 taaaaattaa ggaagaatat caggaaatca tagccttaga gccggattta attattactg
     2221 cagcttatgg acaaattgtt ccagaagcag ttttaaatgc tcctaaaata ggttgtatca
     2281 atgttcatgc ttcattatta cctaaatatc gtggtggcgc gccagtacat tatgcaatca
     2341 tggagggtga agaagttaca ggggtaacga tcatgtatat ggtcaaaaaa atggatgctg
     2401 gaaatattat ttctcaagtc gaagtgccaa ttggagcaga ggaaacaacg ggagaattat
     2461 atgagcgttt gagtattgct ggagcagagt tgttactaga aaccttaccg agcgtacttg
     2521 ctggtacaaa tgagtcaatt gcccaagatg aaagtttagt tacatattca ccaacaatta
     2581 gtcatgaaca agaaaaaata gattttagta agagtgctct tcgagtatat aatcaagtac
     2641 gtggaatgaa tccatggcca ggtgcttata ccacttatca aggtaaagtt gtaaagatct
     2701 gggccggtaa aattcatgta tgtgaaaatg cccttaagca ccacgctcat caagaaaatg
     2761 gaacaatcgt taagatcttt aaagatgcaa ttggggttaa aactggtgaa ggaatatatt
     2821 taattacaga attacagcta gctggaaaga agcgaatgtt agttaaagat tatttgaatg
     2881 gaaataatat ttttgaagtt gatagtaaat tcgaat
//
LOCUS       DAOMTA010000343         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1444_length_2911_cov_2.532913, whole genome shotgun sequence.
ACCESSION   DAOMTA010000343 DAOMTA010000000
VERSION     DAOMTA010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_2911_cov_2.532913"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..1711
                     /locus_tag="PLK68_09355"
     CDS             404..1711
                     /locus_tag="PLK68_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HRM90648.1"
                     /translation="MKYIIRLVSATIVFVLANRLLLGFSNVLLIGLLVGIFIVINAIP
                     YVPYPRPLLKRYRICKAGCELLKIFLISLIMTIVYMLYSFFKVDIGAWLTNLLVVIIV
                     EAVVFWNGIIRIYLTANQLGAKWRIIGVVWGGFPLVNLIILRKLIKIADEIIFENEKT
                     ILNGVRKEQQLCKTKYPLLLVHGVFFRDFKYFNYWGRIPEELEQNGAVIYYGNHQSAA
                     SVIASGDELAKRIKEIVQESGCEKLNIIAHSKGGLDCRYAIAKLNIAPYVASLTTINT
                     PHRGCIFADYLLEKIPEKIKQKVAQGYNTALKKFGDENPDFIAAVNDLTASTCQKFNA
                     DVPDVAEVFYQSVGSKLNVASGGRFPLNFSHHLVKYFDGPNDGLVAENSFPWGCDYTF
                     LTTNTKRGISHGDMIDLNRENIAEFDVREFYVQLVNMLKVHGY"
     gene            1783..2061
                     /locus_tag="PLK68_09360"
     CDS             1783..2061
                     /locus_tag="PLK68_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90649.1"
                     /translation="MEVALVIGMFVCTALALNFLVNGRRKLSGTFEIINLCLIVATAY
                     QFKLVSSIIWVLVILAILLIAGLIFNKKRKDKKVANEIIEARIVDKGE"
     gene            2063..2545
                     /locus_tag="PLK68_09365"
     CDS             2063..2545
                     /locus_tag="PLK68_09365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM90650.1"
                     /translation="METVNIIIEMEDGGVMKGELYPEIAPISVENFLKLIDQDFFAGL
                     IFHRVIPGFMIQGGGYDKDFNHHEADSIKGEFRSNGVANDLLHTRGVLSMARTMVPDS
                     ASSQFFIMHADAPHLDGDYAAFGKITEGLEVVDQIANAPRNIQDCPNQPIVIKTIKRG
                     "
     gene            2550..2804
                     /locus_tag="PLK68_09370"
     CDS             2550..2804
                     /locus_tag="PLK68_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRM90651.1"
                     /translation="MKLFILENCPHCRNARRWIKELQDENPEYNQIEIELIDEQINSE
                     LADQYDYYYVPALFDGNTKLHEGVASKDGLKKIFDQYLGH"
BASE COUNT          989 a          326 c          594 g         1002 t
ORIGIN      
        1 tcagggatgg gtaaagccat atgtgatgat gacgaagaaa gtttatttgc aggaatctgc
       61 catatttgta aagaagattc taaacatagc attattaaca ttggaacaga ggattttgcc
      121 atgattacga tcgttgttga gcaataggtg ctgattttat aatttaagac gatatcaagc
      181 gattagtcgt taagattaat cgcttttttg tatataaata catgattgat agacgaaatc
      241 tgacgggaga atagatagtt ttattgattt ataaaattta ttatataaat caataagtta
      301 agatttcaat gaatgacatt ttaaataaac ctttctgttc ataattgtac taatgataat
      361 ttggcagtat aataagtaaa aatataagag ataaggaaaa attatgaaat atataataag
      421 actggtatca gcaacaatag tttttgtgtt ggcaaatagg ttgttattag gtttttcaaa
      481 cgtattgtta attggtttat tagtggggat ttttatagtt attaatgcaa taccatatgt
      541 cccttatcct agacctcttt tgaaacgata tcgcatatgt aaagcagggt gtgaattatt
      601 aaaaatattt ttaatatctt taataatgac aattgtttat atgctttatt cattttttaa
      661 agtagatatt ggtgcatggt taactaatct attagtggta ataatagttg aggctgttgt
      721 tttttggaat ggcattatta gaatttattt aacggctaac caattaggag ctaaatggcg
      781 cataatcggg gttgtttggg gagggtttcc acttgttaat ttgattattt taagaaagct
      841 tattaaaatt gctgatgaaa ttatatttga aaatgaaaaa actattttaa atggagttag
      901 aaaagaacaa cagctgtgta aaacaaaata tccattgctg ttggttcatg gagtgttttt
      961 tagagatttt aaatatttta attattgggg acgaattccc gaggaactag aacaaaatgg
     1021 agcggtcatt tattatggta atcatcagtc agcagctagt gttattgcta gtggcgatga
     1081 gttagcaaag cgaatcaaag aaattgtaca ggaaagcggg tgtgaaaagt taaatattat
     1141 tgcacattct aaaggtggtt tagattgtcg ttatgcgatt gcaaagctga atattgcgcc
     1201 atatgtggca tcacttacta cgatcaatac tccacatcga ggatgcatat ttgcagatta
     1261 tttattggaa aaaatccctg aaaaaattaa acaaaaagtg gctcagggat ataatacagc
     1321 ccttaagaaa tttggggacg agaatccaga ttttattgct gcggtcaatg atttaactgc
     1381 ttcaacgtgt caaaagttca atgcggatgt tcctgatgtg gcagaagtat tttatcaaag
     1441 tgttggttcg aaactaaatg ttgcttcagg aggtcgcttt ccgcttaatt tttcgcatca
     1501 tttagtgaag tattttgatg ggcctaatga tggattagtg gctgaaaact catttccttg
     1561 gggttgtgat tatacttttt taacaacaaa tacgaaaaga ggaatatcgc atggtgatat
     1621 gatagattta aatcgtgaga atattgctga gtttgatgtt cgggaatttt atgttcaatt
     1681 agttaatatg ctcaaagtac atggttatta gtctggagac acgatagttg ctctggaagt
     1741 caaaatcatg atatgataag aaaggatgaa ggagggttat ttatggaagt cgctttagta
     1801 atagggatgt ttgtttgtac agctttagcc cttaattttt tggttaatgg aagacggaaa
     1861 ttatcaggga cttttgagat aattaattta tgtttgattg tagcaacagc ttatcaattt
     1921 aaattagtta gttcaattat ttgggttctt gtaattttag caatcttact gattgcagga
     1981 ttgatcttta ataaaaaaag aaaagataaa aaagttgcta atgaaataat tgaagcaaga
     2041 atagtagata aaggagaata gaatggaaac agtaaatatt attattgaaa tggaagatgg
     2101 cggagtgatg aaaggtgagt tatatcctga aattgctcca attagtgtgg aaaacttttt
     2161 aaaattaatt gatcaagatt tttttgcagg gttaatattc catcgtgtta tcccaggatt
     2221 tatgattcaa ggtggaggat atgataaaga tttcaaccat catgaggctg acagtattaa
     2281 aggtgaattt agaagtaatg gagtagctaa tgatttatta catactcgtg gtgtattatc
     2341 aatggctaga acaatggttc ctgattcagc atcatcacaa ttttttatca tgcatgctga
     2401 tgctcctcat cttgatggtg actatgcggc ttttgggaaa atcacggaag gattagaggt
     2461 tgttgatcaa attgctaatg ctcctagaaa tatacaagat tgccctaatc agccaatcgt
     2521 aattaaaact attaaaagag gataaggaaa tgaaattatt tattttagaa aattgtcctc
     2581 attgtcgtaa tgcacggcgc tggattaaag aattgcaaga tgaaaaccca gaatataatc
     2641 aaattgaaat tgaattgatt gatgagcaaa tcaatagtga attagcagat cagtatgatt
     2701 attattatgt tccagctttg tttgatggga atactaagtt acatgaaggt gttgccagca
     2761 aagatggttt gaaaaaaata tttgatcaat atttaggaca ttagtttgtc ctttttattt
     2821 taaatttaat atagtgtatg agtaatcaaa gaatcttatt actatttgat gttttattgt
     2881 aatatagaca aaaagcctcg ctcaagaggc t
//
LOCUS       DAOMTA010000344         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1445_length_2911_cov_1.855392, whole genome shotgun sequence.
ACCESSION   DAOMTA010000344 DAOMTA010000000
VERSION     DAOMTA010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_2911_cov_1.855392"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLK68_09375"
     CDS             <1..368
                     /locus_tag="PLK68_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90652.1"
                     /translation="QYKKLLIENDQESLFTSTLGRVFSFSPVGDDGHEPCVAVRKMVE
                     KHGDAKMISSYQTAVFNRRGIFSPSAGKEELKMAEEFKENAEYLEPNYPKTAQIFYGL
                     FESYKCESKQERMDAENGW"
     gene            911..2137
                     /gene="pnuC"
                     /locus_tag="PLK68_09380"
     CDS             911..2137
                     /gene="pnuC"
                     /locus_tag="PLK68_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRM90653.1"
                     /translation="MIKFFKVNIEPKKRLRMIEVIFSIGLCIASIISIGYGLFDINAN
                     IEDIQFKQSIQMTRDTDLEDYSEDNTICDVTYINGDKQLIVSYSYEDYVKLDDKTITA
                     YEYETKNGTKLYFDHQNINDQEVQYAYKQVRANELASLFNFGIASLILMLSILIMMLF
                     AKQFTTYEKSWFISIMVLATIFSVVFPEESANGVNGIIIMLLYLLDTFLNILCELLIS
                     KQSRYNFLVSVLVEIVEIAICVVLMYRFATMATTLFFWLPIDIISYINWSKHKDDEEN
                     ELTVVRKLRGYQEVLVIIGIIVWTFVIGYLISGLNIATDFYNNELLETFIIYIDACAS
                     AVGIANGLFIFFRLQEQWIAWYICAFLEAVINIISGQYVLLVLKLGYFTNTTYGYIKW
                     SRYIKEHTTEKQAQIS"
     gene            2212..2769
                     /gene="efp"
                     /locus_tag="PLK68_09385"
     CDS             2212..2769
                     /gene="efp"
                     /locus_tag="PLK68_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRM90654.1"
                     /translation="MAILAGDFKTGLTLIVDGDPCQVMDFQHVKPGKGAAILKTKMRN
                     LKTGAIQERNFNASTKFEAANISRKDAQYSYEADATFYFMDLETYETYELAEDQVGDN
                     KYYIIEGSTVSLMFFDGLLLSVSVPEKVELTVVETDPAIKGAPSNQTKDAVTDTGLTL
                     RVPQFIDTGEKIVVFTTDGKYAGRA"
     gene            2744..>2911
                     /locus_tag="PLK68_09390"
     CDS             2744..>2911
                     /locus_tag="PLK68_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM90655.1"
                     /translation="MESMLEELNKLFFKIMNKIVLITGGAKGIGKAIALELAKQGYDI
                     VINYLTSKKEAE"
BASE COUNT         1071 a          337 c          539 g          964 t
ORIGIN      
        1 agcaatacaa gaaattatta attgagaatg accaggagag tttgtttaca tcaacacttg
       61 gcagagtttt ctctttttct ccggtaggtg atgatgggca tgaaccatgt gttgcagtaa
      121 gaaagatggt tgaaaaacat ggtgatgcta agatgattag cagctatcag actgctgtat
      181 tcaatagacg cggtattttt agtccttcgg caggaaaaga agaattaaag atggctgagg
      241 aatttaaaga gaatgcggaa tatttggaac caaattatcc taagacggca caaatcttct
      301 atggattatt tgaaagttat aagtgtgaat ctaaacaaga gaggatggac gctgaaaacg
      361 gctggtagta gcatctattt tgaaacctat ttaacaataa aatggaaatt ggatacatat
      421 cttattaaga aaaaatagat aagaggtgca tttgatacac aacaaattat tgaaggattt
      481 tatatgggag aatctatggc ttttaatcaa aaatggttag gaatattttt acaacttcgt
      541 attagtaaac cttttggaga gctgtatcta aatttgtata tgcttatgtt aaagatttgt
      601 ttgattttct gaaaaactca attataataa gtggtccttt taattgtatt aacgaagcaa
      661 tataaagtgt actaaataaa gtatcgaatt tatattaaac aattgcataa aaaaggggct
      721 gtaacgaaat aaaatagtta agccctaaaa caaaaaagtc aatagctcag tttactgatt
      781 aatattgact tttttaatac actatattat tttgtgctta ctatttataa aaaagtattt
      841 catatttata aaaatatatt ataatgtaaa agcattaaaa atgtaaatat ataggggaag
      901 aaggtttgat atgattaaat tttttaaagt aaacatagaa cctaaaaaaa ggttgagaat
      961 gatagaagta atattttcaa taggattatg tattgcttca attatatcaa ttggatatgg
     1021 cttgtttgat attaatgcta atattgaaga tatacagttt aaacaaagta tccagatgac
     1081 aagagatact gacttagaag actacagtga agataacacg atttgtgatg ttacctatat
     1141 aaatggtgac aagcagctaa ttgtatctta ttcatatgaa gactatgtta aattagatga
     1201 taagacaatt acagcatatg aatacgaaac aaaaaatggg acaaaattat attttgatca
     1261 ccaaaatata aacgatcaag aagtacagta tgcctacaaa caagtaagag ctaatgaatt
     1321 agcctcatta tttaactttg ggattgcttc tttaatcttg atgttatcca ttttaattat
     1381 gatgttattt gcaaaacaat ttacaaccta tgaaaaatca tggtttattt cgatcatggt
     1441 tctagcaacg attttttccg tagttttccc agaagaaagt gctaatgggg taaatggaat
     1501 tatcattatg ctgctttatt tattggatac attcttaaat attctttgtg aattattgat
     1561 ttctaaacaa tcacgatata attttcttgt ttcggtatta gttgaaattg ttgaaattgc
     1621 tatatgtgta gtattaatgt atcgatttgc tacaatggca acaacattat ttttctggct
     1681 gccaattgat attattagtt acataaattg gtcaaaacat aaagatgatg aagaaaatga
     1741 attaacagta gtaagaaaac taagaggata ccaggaagtt ttagttatca tcggaatcat
     1801 tgtttggacg tttgtaattg gatatcttat tagtggttta aatattgcca cggatttcta
     1861 caacaatgaa ttattagaaa cattcattat ctatattgat gcttgtgcct cagcagtagg
     1921 gattgcaaat ggtttattta ttttctttag gttgcaagag cagtggattg catggtatat
     1981 ttgtgcattt cttgaagccg ttataaatat tatttcggga caatatgttt tattagttct
     2041 aaaactaggc tattttacta atacaacata tggttatatc aagtggagca gatatattaa
     2101 ggaacataca acagagaaac aagcacaaat atcataatgt gtcttgagga atgatgttaa
     2161 aattgataga ataaagtata tattaatatg agcaagatta ggagtgaaaa tatggctata
     2221 ttagcaggtg attttaaaac aggattaact ttgattgttg atggtgatcc ttgtcaagtt
     2281 atggattttc aacatgtaaa accaggtaaa ggagcagcca tcttaaaaac aaagatgaga
     2341 aacttaaaaa ctggagcgat tcaagaaaga aactttaatg cttcgactaa gtttgaagca
     2401 gcaaatattt caagaaaaga tgcgcaatat tcatatgaag cagatgctac tttttatttt
     2461 atggatctag aaacatatga aacatatgaa ttggcagagg atcaagtagg ggacaataaa
     2521 tattatatta ttgaaggttc aactgtttca ttaatgttct ttgacgggtt attgttatca
     2581 gtatctgttc ctgaaaaagt agaattaacg gtggttgaaa cggatccagc catcaagggt
     2641 gcaccatcta accagacaaa agatgccgtt actgatacag ggttaacatt acgggttcct
     2701 cagttcattg atactggaga aaaaatcgtt gtgtttacaa ctgatggaaa gtatgctgga
     2761 agagcttaat aagctctttt ttaaaattat gaataaaatt gtattgatta ctgggggggc
     2821 taaaggaatt ggcaaagcaa ttgctttaga attggcaaaa caaggatatg atattgtaat
     2881 taattattta acatctaaaa aagaagctga g
//
LOCUS       DAOMTA010000345         2904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1450_length_2904_cov_2.290628, whole genome shotgun sequence.
ACCESSION   DAOMTA010000345 DAOMTA010000000
VERSION     DAOMTA010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_2904_cov_2.290628"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..312)
                     /locus_tag="PLK68_09395"
     CDS             complement(154..312)
                     /locus_tag="PLK68_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90656.1"
                     /translation="MAEGDYKEVYFDQYCKKCKDEKTPEGNDPCNECLTNSENVDSHK
                     PVNWKAKK"
     assembly_gap    455..515
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1540..1734)
                     /locus_tag="PLK68_09400"
     CDS             complement(1540..1734)
                     /locus_tag="PLK68_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90657.1"
                     /translation="MIQFMKIGAAFLYMVLIFVASAILGMIISDINTAHGTKHGVSWV
                     SAGYFITLLIAATAFYMFIN"
     gene            complement(1738..2508)
                     /locus_tag="PLK68_09405"
     CDS             complement(1738..2508)
                     /locus_tag="PLK68_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90658.1"
                     /translation="MTRYQKKDNINNTWAEKEIKRAIELTDPDYIPRKVGLVERALEK
                     IGNEPLKKDEFLGYETACYLTALNAFHEIESGEIGNTSFIRDTLIRLMDGLPLTPIDD
                     DPETWINMDDERGWKYPTHTVFRSTRYFHLYKIVLKNGEVRYEDARSVLLVDDSENVI
                     SKPLLCLENGFYNNPAEIIVNELFPILLPYIPSKEQFKVVCFYSDDLFWVKRIIDKSG
                     NAITVNRYYQLILDAPKHMAWVEIGKTKYYHRLKNKGE"
     gene            complement(2508..2768)
                     /locus_tag="PLK68_09410"
     CDS             complement(2508..2768)
                     /locus_tag="PLK68_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90659.1"
                     /translation="MITIYMFSVIFYYVLLVLLFFYETIKMFMKGCLIHWHNMPFGFI
                     MRFLLFGMLPFVNVLGAIKIIALFKQMELYGHELSEKEKESK"
BASE COUNT          854 a          589 c          370 g         1030 t
ORIGIN      
        1 atcaacagat gccttcataa tttcttctgt gacatctaca taatgtttac cgtttgcggt
       61 tactctttca tcaactttat tcataccgat ttcctcctaa tataagtgtt tagtttccat
      121 aataggagtc ataaatatcg cgaagttact ttatcatttc ttcgccttcc agtttaccgg
      181 cttatgagaa tccacatttt ctgaattggt caggcattca ttacaaggat cgtttccttc
      241 aggtgtcttc tcgtccttac acttcttgca atattgatca aaatatactt ctttgtaatc
      301 gccctcagcc attactgttt acctcctgcc aatttacagt attttcatct tcctgtccaa
      361 tcggactgaa ctgcccgtat tcatcctcag caataaggca tttaccatac gtatgagtgt
      421 cagcaacacc aacaatggtt ataacctttc cactnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgct tgtaggtttt tccatattga
      541 aatatcattt ttctcctttc aagaagcaaa cttactttcg ttgaatttct ttttgttgcg
      601 caacgcttta ctgatagcca ggtctattcc agatcggctc tttagatggt agtaatataa
      661 atctttataa gcagtattca atcgatcaat tcgcccactg ctttgtacca tgactttgta
      721 cgaataattc tgagagaaga atagtatctg tcttaatgca attccaacct tcggcacctg
      781 cggtatactg taccaaatat acccagttct tactctctgg tatttcctga tgctgatgct
      841 tatgcccatt ccattctgct acctcaaatt cttttcgccg accttttcaa agtttttctt
      901 gctgccattt ttctctcatt ttgagggtta gtcttctttc catggtactt cttccagttc
      961 atataattga tggtccatat aatatcttcg ataaatatca gcttccttac caagttcttt
     1021 tactgtctct gaccccagct atttttagga tttcacatcc ccctaacagt ggtatccaaa
     1081 tcaactttca tcaccaacaa ctcctttttc caaaatgata atcaaatact tcattttcag
     1141 caatcattta tttttaccta ttttcttttt gaaggtatct ttcttacaat attcaaatga
     1201 acaacatccg ggaacaaaac ccaaaatatc tttgttagta taatacttac aatgattaca
     1261 ttcctttatt tttgcacaac atttccagaa agagtaactt tggaagatgg ggttaaattg
     1321 tttttctttt taaactgata gataagattt gattttgcct tattaacagt ttcagcatat
     1381 gtttcgccat gccaagagtt acaaatacag ttaccaaaca taacgactgg gccatcatat
     1441 gtgtatttaa ccataattta ttcctttcct attccactaa gctcagtatc tacagttaca
     1501 attctattaa tcataagaaa tcccctcaag aatataatgc taattgataa acatataaaa
     1561 ggcagtcgca gctattaata gggttatgaa atacccagcc gagacccaag aaaccccatg
     1621 cttagttcca tgggcagtat ttatatcact aataatcatt ccgagtatcg cagaagcaac
     1681 aaaaataagt accatataaa gaaaggcagc tccaattttc ataaactgta tcataattta
     1741 ttctccttta ttttttaatc tatgatagta cttggttttt ccaatttcaa cccaagccat
     1801 atgttttgga gcatccagta taagttgata gtagcgatta actgtaatag cattaccact
     1861 tttatcaatt attcttttta cccagaacaa atcatcagag tagaaacata ctaccttaaa
     1921 ttgctcttta cttggaatat atggtagcaa tattggaaat aattcattta ctattatctc
     1981 agctggatta ttataaaatc cattttctaa acataataat ggtttcgaaa ttacattttc
     2041 agaatcatca accagcaata ctgaacgagc atcttcatac ctaacttctc cattttttaa
     2101 aacaattttg tatagatgaa aatatctggt ggatcggaaa actgtatgtg tcggatattt
     2161 ccaaccacgt tcatcatcca tattaatcca ggtttcaggg tcatcatcaa ttggtgttaa
     2221 cggaaggcca tccattaatc taatcaatgt atcccgtatg aaacttgtat tcccaatttc
     2281 ccccgattct atttcatgaa atgcattaag tgctgtcaag taacaagcag tctcataacc
     2341 aaggaactca tcctttttta aaggttcatt tcctattttt tccaaagccc gttctactaa
     2401 acctactttt cttggaatat aatccgggtc tgtcaattca atagctctct ttatttcttt
     2461 ttctgcccat gtgttattaa tattatcttt cttttgataa cgtgtcatta tttgctctcc
     2521 ttttcttttt cactaagttc atggccataa agttccatct gtttaaataa agctatgatt
     2581 ttaattgctc ctaagacatt aacaaaagga agcatcccga ataataaaaa cctcattatg
     2641 aaaccaaaag gcatattatg ccaatgaata aggcatcctt tcataaacat tttgattgtc
     2701 tcataaaaga ataataatac taataataca taatagaaaa tgacactgaa catataaata
     2761 gtaatcatgg tattagtctc tcctttttat tttgcttatt attgaatgtc accttttttg
     2821 agtgattctt taagctgccc caaaatagat tcgacagtaa ctcttgtttc tgcttccata
     2881 agaattgtat ctttatgaga atca
//
LOCUS       DAOMTA010000346         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1452_length_2901_cov_2.110330, whole genome shotgun sequence.
ACCESSION   DAOMTA010000346 DAOMTA010000000
VERSION     DAOMTA010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_2901_cov_2.110330"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLK68_09415"
     CDS             complement(<1..687)
                     /locus_tag="PLK68_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM90660.1"
                     /translation="METGRYGIHGGQYIPETLMNEIHNVEKAYEFYKNDPEFNRELEK
                     LLKEYAGRPSLLYYAKKMTEDLGGAKVYLKREDLNHTGSHKINNVLGQVLMAKKMGKT
                     RVIAETGAGQHGVATATAAALLGLECEIFMGKEDTDRQALNVYRMELLGATVHSVTSG
                     TMTLKDAVNETMREWTKRVDDTLYVLGSVMGPHPFPMIVRDFQSIISKEAREQILEDE
                     GKLPTAVMACV"
     gene            complement(674..>1270)
                     /locus_tag="PLK68_09420"
     CDS             complement(674..>1270)
                     /locus_tag="PLK68_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRM90661.1"
                     /translation="MQIKICGLFQVEDIDYVNEAKPDYVGFVFAKSKRQVDIHQAEKL
                     KNKLDTNIKAVGVFVDEQISEITAIVKMGIIDLIQLHGHEDNAYIKQLKQSVQMPIIK
                     AIKVIEKDDLNNLDYECDYYLLDSKISGSGKSFDWSLIKDLDKPFFLAGGIDLDNLDE
                     AMSKADYGIDVSSGVETNGIKDRNKIIEIVRRTKNGNR"
     assembly_gap    1271..1290
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1291..2068)
                     /gene="trpC"
                     /locus_tag="PLK68_09425"
     CDS             complement(<1291..2068)
                     /gene="trpC"
                     /locus_tag="PLK68_09425"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRM90662.1"
                     /translation="MIIDDIVERTLERVAENKKRNSIEKLAKLAFDKPINKDYPFERA
                     LRRAGISYIMEIKRASPSKGVIAPNFDYKSIAKEYEDIGAAAISVVTEPDFFKGDDDF
                     LAEIAKIVKIPVLRKDFVVDEYMIYEAKLLGVDAVLLICSILDEITLMRCLNLAERLG
                     MSALVEAHSSMQVKKALRVGAKIIGVNNRDLRNFEVDLNNSIELRSMVPENIIFVSES
                     GIKTYDDIKTLEENNVDAVLVGETLMRSHDKRKTFEILQGL"
     gene            complement(2082..>2901)
                     /gene="trpD"
                     /locus_tag="PLK68_09430"
     CDS             complement(2082..>2901)
                     /gene="trpD"
                     /locus_tag="PLK68_09430"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRM90663.1"
                     /translation="HCIKLLNDKDVLEIVGTGGDGSNSFNISTTSSLVIAASGVKVAK
                     HGNRAASSKCGAADVLEALGVKIDVTPEKSATILNDINICFLFAQNYHIAMKYVAPVR
                     RELGIRTVFNILGPLTNPAGANMQVMGVYEEALVEPLAEVLTNLGVKRAMVVYGQDSL
                     DEISLSAPTSVCEVKDGTYTRYTITPEQFGLTRCCKEELVGGTPKDNAKITRDILSGV
                     KGPKRDAVLLNAGAAIYIAGRTKTMQEGIELAARLIDDGKALAKLEEFINKTNM"
BASE COUNT          880 a          641 c          344 g         1016 t
ORIGIN      
        1 aacacaagcc ataacagctg ttggtaactt tccttcatct tctaagattt gttcacgtgc
       61 ttccttagaa ataatacttt gaaaatcccg aacaatcatc ggaaatggat gtggtcccat
      121 tactgagcct aatacatata aagtatcatc aactcttttt gtccactcac gcattgtttc
      181 attaacagca tcttttaatg tcattgttcc acttgttaca ctatgaactg ttgcacccaa
      241 caactccatt cgatacacat ttagagcctg acgatcagta tcttctttgc ccataaaaat
      301 ttcgcactct aaacctagta aagcggcagc cgtagctgtt gcaactccat gctgaccagc
      361 cccagtttca gcaataacac gagttttccc catctttttt gccatcaaaa cttgacctaa
      421 aacattattg attttatgag atcccgtatg atttaaatct tcacgtttta aatatacttt
      481 cgcaccacct aaatcttctg tcattttttt agcataatat aacaacgatg ggcgccctgc
      541 gtattctttc aataattttt ctaactctcg attaaactca ggatcatttt tataaaattc
      601 atatgctttt tcaacattat ggatttcatt cattaatgtt tctggaatat attgaccccc
      661 atgtattcca tatctacctg tttccatttt ttgttctcct tactatttct ataattttat
      721 tacgatcttt gattccgtta gtttctaccc cactactaac atctattcca taatcagctt
      781 tactcattgc ctcatctaaa ttatctaaat caattcctcc cgctagaaaa aatggtttat
      841 ctaaatcttt gattagtgac cagtcaaatg acttaccgct accgcttatt ttactatcca
      901 gtaaataata atcacattca taatccaggt tattaagatc atccttttca atcaccttga
      961 tggctttaat aatcggcatc tgcactgact gttttaactg cttaatataa gcattatcct
     1021 catgaccgtg aagttgaata agatcaatga tccccatttt aacaatagca gtaatttcac
     1081 taatttgttc gtcaacaaat accccaacag cctttatatt tgtatcaagt ttatttttta
     1141 atttttctgc ttgatgaata tctacttgcc ttttactttt agcaaagaca aatccaacat
     1201 aatcaggttt tgcctcattt acataatcta tatcttctac ttgaaataag ccacaaattt
     1261 taatttgcat nnnnnnnnnn nnnnnnnnnn gtaatccttg taagatttca aaagtcttac
     1321 gtttatcatg agatcgcatt aaagtttctc caactaatac agcatcaaca ttattttctt
     1381 ctaacgtttt tatatcatca tatgtcttaa tcccactttc agaaacaaaa ataatatttt
     1441 ctggtaccat cgaacgtaat tcaatactgt tatttagatc aacttcaaaa tttctcaaat
     1501 cacggttatt aacaccaatg atcttagcac caacacgcaa tgccttcttt acctgcattg
     1561 aactatgggc ttcaaccaaa gcactcatcc ctaaacgctc tgctaaattc aaacagcgca
     1621 ttagcgttat ttcatcaaga atactgcaaa ttagtaatac ggcatctaca cctaatagtt
     1681 tagcttcata aatcatatat tcatctacaa caaaatcttt tctaagtact ggtattttga
     1741 ctattttagc aatttcagct aaaaagtcat catcaccttt aaaaaagtcg ggctctgtaa
     1801 ctactgaaat tgcagctgca ccaatatctt catattcctt tgcaatactt ttataatcaa
     1861 aatttggagc aatcactcct tttgacggtg aagcccgttt aatctccata atataagaaa
     1921 ttccagctct tcttaaagct cgttcaaaag gataatcttt attaattggc ttatcaaaag
     1981 ccaatttagc taatttttca atggaattac gttttttgtt ttctgctact ctttccaaag
     2041 ttctttctac aatatcatca ataatcattt taaaactcct tctacatgtt agttttgtta
     2101 atgaactcct ctagtttagc taatgcttta ccatcatcaa tcaatctagc agctaattcg
     2161 attccttcct gcattgtttt tgtacgaccg gcaatataaa tagcagctcc cgcattcaac
     2221 aacacagcat cacgtttagg tcctttgaca ccacttaaaa tatcccttgt aattttagca
     2281 ttatctttag gggtaccacc tactaattct tctttgcagc agcgtgttaa tccaaactgt
     2341 tctggagtta ttgtataacg agtataagta ccatctttaa cttcgcatac acttgttggg
     2401 gctgataatg atatttcatc taaactatct tgtccatata ctaccatggc acgtttaact
     2461 cctaaattag ttaacacttc tgccaatggt tcgactaatg cctcttcata tacacccatt
     2521 acctgcatat tagcacctgc cggattagtc aaaggcccta aaatattaaa cactgtccta
     2581 attcctaatt cccgtcttac tggagccaca tatttcattg caatatgata attttgagca
     2641 aacaagaagc aaatattaat gtcatttaat attgtagcac ttttttcagg agttacatca
     2701 atttttactc ccaaagcttc taaaacatca gcagcaccac atttagagct agctgcccgg
     2761 ttcccatgtt tagccacttt tacaccactt gctgcaataa caagagaaga tgtcgtagaa
     2821 atattaaatg agtttgaacc atctccacca gtaccaacaa tctccaaaac atctttatca
     2881 tttagtagtt tgatacagtg t
//
LOCUS       DAOMTA010000347         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1454_length_2899_cov_2.457454, whole genome shotgun sequence.
ACCESSION   DAOMTA010000347 DAOMTA010000000
VERSION     DAOMTA010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1454_length_2899_cov_2.457454"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /gene="pgk"
                     /locus_tag="PLK68_09435"
     CDS             complement(<1..164)
                     /gene="pgk"
                     /locus_tag="PLK68_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRM90664.1"
                     /translation="MDKKTIKDIEVAGKVVLCRVDFNVPRNKETGEITNDNRVVAALP
                     TINYLLEQAP"
     gene            249..560
                     /locus_tag="PLK68_09440"
     CDS             249..560
                     /locus_tag="PLK68_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM90665.1"
                     /translation="MENSQLISVDKLPELLKHDYTFIDLRDPLQFNKIHLRKFINIPY
                     DTFIATPPQFPKDKPIYLICYSGKRSLDLAQKLTRCGYHAYSFNGGFYAVEHPINKQF
                     Y"
     gene            667..1791
                     /locus_tag="PLK68_09445"
     CDS             667..1791
                     /locus_tag="PLK68_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90666.1"
                     /translation="MIEFKNIYKSFKDKHVLEDVSFSIDKGEFVCIIGPSGCGKTTAL
                     KMINRLIKPTRGAIYVDGKDISKEDEIDLRRNIGYVIQQTGLFPHMTVKENIELIPKL
                     KNKKDPSLATKVVELLDMVGLDPEGYMNLYPTQMSGGQQQRVGVARAFASDPEIILMD
                     EPFSALDPITREQLQDELLTLQNQLHKTIIFVTHDMAEAIKMADRICIMSDGRVQQFD
                     TPEQILKHPANDFVHNFVGKKRIWDSPELIKVADIMIDKPITCNVNLKCIKAVNIMYN
                     YKVDSLMIVDNHQNFLGILDANQAAREKNRDKKVDEVMHTECLSVKPDESIVDVINLA
                     NSSNIYTLPVVDDKNKLVGLITKSTLVTTLSKKYDESEDD"
     gene            1793..>2899
                     /locus_tag="PLK68_09450"
     CDS             1793..>2899
                     /locus_tag="PLK68_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HRM90667.1"
                     /translation="MNEFINYFSQNFSTIFGLFIDHIQLTILAIIISILIGVPLGIII
                     TYFKPSKKPVMAIANIIQAIPSMALLGFMIPLLGIGTKPAIVMVILYSLLPIIKNTVA
                     GLDSINSDTLEAAKGIGLTPMQVLYKVQIPLAAPVIMAGVRISAVSSVGLMTLAAFIG
                     AGGLGYLVYAGIRTVNNAQILAGAIPACILALLIDYIFSILEVLVTPKCNQLASPQSK
                     GKKLIDKIVIIATCICLAGSFVYTNLGKTSDKITINIGSMDFSEQEILNYMLKYLIEK
                     NTDVEVNQSLSLGSSSIVLDAMKTGDVDMYVDYTGTIYGSVLGLEPNSDVEAVYNTVK
                     DEMKKQYNFTVLEPLGFNNTYTLAMSKQTADKYNI"
BASE COUNT          988 a          498 c          495 g          918 t
ORIGIN      
        1 tttggagctt gttctaatag ataattgatt gtaggtaaag cagctaccac acgattatca
       61 tttgtaattt ctccagtttc tttgtttctt ggtacgttga agtcaacacg gcataaaact
      121 acttttccag cgacttcgat atctttgatt gtttttttat ccatctttaa tattcctcca
      181 tggctgaatt atatcactgt tattcattgt tttcaattaa tttcatacaa tataatgagg
      241 tgaaatatat ggaaaacagc caattaatca gtgtcgacaa attacccgaa ttactaaaac
      301 atgattatac atttatcgat ttaagagatc ctttacaatt taataaaatt catttgcgca
      361 agttcatcaa tatcccttac gatactttta tcgccactcc accacaattc cccaaagata
      421 agcctattta tttaatctgt tacagcggca aaagatccct tgatctagct caaaaattaa
      481 cgcgctgtgg ttatcacgca tacagcttca acggcggttt ttatgctgtt gaacacccca
      541 tcaataaaca attttattga tgaaatcatg ctaatttatt atcacaacaa tagaagtaaa
      601 ttaccatatt cacataaagt acacattttc tgtgatataa taggtactta agaagggaat
      661 gattaaatga ttgaatttaa aaacatctat aagtctttta aggacaaaca tgtactagaa
      721 gacgtttcgt tttcaattga taagggagaa ttcgtttgta tcatcggtcc tagtggctgt
      781 ggtaaaacta ccgctttaaa aatgatcaac cgcttgatca agcctactcg tggggctatc
      841 tatgttgacg gtaaagacat cagcaaagaa gacgaaattg atttaagacg aaatattggt
      901 tatgtaattc aacagacagg attatttccc catatgactg tcaaagaaaa catcgaatta
      961 ataccaaaat taaaaaacaa gaaggatccc agccttgcca ccaaggttgt tgaactattg
     1021 gatatggtag gactcgatcc agaagggtat atgaacttat accctactca aatgagtggc
     1081 ggtcaacagc aaagggtcgg agttgcccga gcgttcgcca gtgatccaga gatcatctta
     1141 atggatgagc catttagtgc tcttgatcca atcaccagag aacaactcca agatgagttg
     1201 ctaacgttac aaaaccagct acataaaacc attatttttg taactcatga tatggctgaa
     1261 gcaatcaaga tggctgatcg aatttgtatt atgtctgatg gccgtgtcca acaatttgat
     1321 acgcccgaac aaattttaaa gcatcctgct aatgattttg ttcataactt cgttggaaaa
     1381 aaacgaatat gggattcgcc agaattaatc aaggtagctg atatcatgat tgataagcca
     1441 attacttgta atgtcaattt aaaatgtatc aaagctgtaa acattatgta taattataaa
     1501 gttgatagtt tgatgatcgt tgataatcat caaaactttt taggaattct tgatgctaat
     1561 caagcagcta gagaaaagaa tcgtgataaa aaggtcgatg aggtaatgca taccgaatgt
     1621 ttatcagtta aacccgatga atctatcgtt gatgtcatca atctagctaa cagcagtaat
     1681 atttacactt tgccagtagt tgatgataaa aacaaattag ttggattaat tactaagagt
     1741 acattagtaa ccactttatc aaagaaatat gatgaaagtg aggatgatta aaatgaatga
     1801 atttattaat tactttagtc aaaacttttc aactatcttc ggactcttta tcgatcatat
     1861 tcaactaacg attcttgcta ttattatttc aattttaatt ggagttccac taggaatcat
     1921 tattacttat tttaaaccat caaaaaaacc agtcatggca attgcaaata ttattcaagc
     1981 aattccatca atggccttgt taggattcat gattccttta ttaggaatag gaacaaaacc
     2041 agcgattgta atggttatct tgtattcgtt attaccaatt ataaaaaata ccgtagctgg
     2101 attggatagc attaatagcg atacccttga agcagctaaa ggaatcggat taacaccaat
     2161 gcaagttctt tataaagttc aaattccttt agcagcacct gttatcatgg ccggagttag
     2221 aatctcagca gtcagttcag tcgggttaat gactctagca gcttttattg gtgccggagg
     2281 attagggtat ttagtctatg cgggaatccg aacagtaaat aatgcacaga ttctagccgg
     2341 agctattcct gcctgtatct tagcattatt gattgattat attttttcaa ttttagaagt
     2401 cttagttacc cctaaatgca atcaactagc cagtccacaa agcaaaggaa aaaaacttat
     2461 tgataagatc gttattatcg ctacttgtat ctgtctagct ggaagttttg tttatactaa
     2521 tttaggcaaa actagcgata aaatcacaat caatattggt agtatggact tttctgaaca
     2581 agaaatattg aactatatgt taaagtattt gattgaaaaa aataccgatg tagaagtaaa
     2641 ccagtcatta tcacttggct cttcgtcaat cgtcttagat gcgatgaaaa ctggtgatgt
     2701 cgatatgtat gtcgattata caggaactat ttacggtagt gttctcggcc ttgagcctaa
     2761 cagtgacgta gaagctgtct acaacacagt caaagacgaa atgaaaaaac aatataattt
     2821 taccgtttta gaaccactag gatttaacaa cacttatacc ttagcaatga gcaagcaaac
     2881 tgctgataaa tataacatc
//
LOCUS       DAOMTA010000348         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1456_length_2897_cov_2.294511, whole genome shotgun sequence.
ACCESSION   DAOMTA010000348 DAOMTA010000000
VERSION     DAOMTA010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_2897_cov_2.294511"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..305
                     /locus_tag="PLK68_09455"
     CDS             168..305
                     /locus_tag="PLK68_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90668.1"
                     /translation="MKELSITSQQKINGGGAIVYVISLVAVAAGYSVAKLLRKRFSIT
                     R"
     gene            355..741
                     /locus_tag="PLK68_09460"
     CDS             355..741
                     /locus_tag="PLK68_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRM90669.1"
                     /translation="MKIVRLVVVLAVALSICFCGIYLKFFSTINSYINTYIVQVGIYK
                     QDENANEMIKKLSDLGKPNYKYQKDNNFVVITGVFLDEKEAKDMGNTISESGITCVIK
                     QTKFESSFKEVIEKQDYEAIIKELGS"
     gene            738..1406
                     /gene="radC"
                     /locus_tag="PLK68_09465"
     CDS             738..1406
                     /gene="radC"
                     /locus_tag="PLK68_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRM90670.1"
                     /translation="MKLKNYPLEERPREKAFHHGIESLNNIELLALVLRTGNKQESAI
                     ELAQRVINEIGGFRYLHDINYYQLIQIKGIKQAKAIEVLAIIEIAKRLDKQPVAMSAI
                     KEPRDGYELLKNQLMFEQQEKVIVLCLNSRLEVIKEKTVFIGGNNISIISGRELFKEA
                     LICGSNRVMVVHNHPSGNPEPSIEATERLYSMAKELDIDVVDHLIIGRSRFYSFASNK
                     IIEV"
     gene            1464..2309
                     /gene="mreC"
                     /locus_tag="PLK68_09470"
     CDS             1464..2309
                     /gene="mreC"
                     /locus_tag="PLK68_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRM90671.1"
                     /translation="MNNRKKQRNKRIVLIITVAIVVFSTISLVFSRTQGGLERMVSDS
                     IAAIEYYVVKKPVEFLSNLFSEYNELKDVYKENKILKAKLSSYASVEVNTDVLSKEID
                     ELKKMLNIEYLPTDYNVKTTSFVRESDDWNNEITIDLGSLAGVSKDMVVISSKGMIGK
                     VTSVTEVTARVQLLTAENPTSALPIQVINGDQNVYGLLNRYDIESKCFEITLFSDVEK
                     FEDNAKVITSGLGGKAPKGIYIGTVESSIVSEDGTSKTIRVKPAADFNDLSYVAVVFR
                     SDSNE"
     gene            2302..2835
                     /gene="mreD"
                     /locus_tag="PLK68_09475"
     CDS             2302..2835
                     /gene="mreD"
                     /locus_tag="PLK68_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRM90672.1"
                     /translation="MNKQLYRYYLVMALSFVVDSIISYYLPYNFDKLGITIVPCVSLM
                     MFTLLNNTIGDEHRYIFATVTGLYYAIVYANSLLIYVLLYCVYAFFGKKYTKLATYTL
                     LEMFIAVIVTIIAQEVVIYWLVWITNVTQLSIVAFLTNRLLPTLGANLLLIAPVYFIH
                     KKLGFEGKVNAYQSKRS"
BASE COUNT         1012 a          320 c          586 g          979 t
ORIGIN      
        1 aagaaaaatg aatgaattac agcaatgtat gcaaatggaa gtaatgggcg gtggtgttac
       61 gggatttttc ttttgtgtcg taattggaac tgcaatttat aagatgtttc gttcgggggc
      121 tggacgtgta tcgattccta aaattatttc aatcgagtgg cgttaaaatg aaagaattat
      181 cgattacatc tcagcaaaag atcaatggtg gcggagcgat tgtttatgtg atttctttag
      241 tagctgtagc tgctggttat agcgttgcta aattattaag aaaacgtttt tcaattacac
      301 gttaattgag catatttata aactgctaac atatactaga tttggaggtg caagatgaaa
      361 atagtaagac ttgtagtagt gttagcagtt gctttatcaa tttgtttttg tggaatctat
      421 ttgaagtttt tttcaacgat caatagttat attaatactt acatagttca agtaggaata
      481 tataaacaag atgaaaacgc taatgaaatg ataaaaaagt tatcagattt agggaaaccg
      541 aattataagt atcaaaaaga taataatttc gtagtgataa caggtgtgtt tttggatgaa
      601 aaggaggcaa aggacatggg gaatacaatt agtgaaagtg gaatcacatg tgtaataaaa
      661 caaacgaaat tcgaaagtag ttttaaagag gttattgaaa aacaagatta tgaagcaatt
      721 attaaagaat taggatcatg aaattaaaaa actatccatt ggaagaacga ccgcgcgaaa
      781 aagccttcca tcacggaatt gaaagcctaa ataatattga attattagca ttagtcttaa
      841 gaactggaaa taagcaagaa tcagcgattg aattggctca acgtgttata aatgaaattg
      901 ggggttttcg ttatttgcat gatatcaatt attatcagtt gattcagatt aaaggaatca
      961 aacaagctaa agcaatagaa gttttagcta ttattgaaat tgctaaacgt ttggataaac
     1021 agcctgtggc tatgtcagct attaaagaac cgcgtgatgg ttatgaattg ttgaaaaatc
     1081 aattaatgtt tgaacagcag gaaaaagtga ttgtattatg tttaaattca cgcttagagg
     1141 taattaaaga aaaaaccgta tttattggtg ggaataacat atctattatt agtgggagag
     1201 agctgtttaa agaagcattg atctgtggta gtaatcgtgt gatggtcgtt cataatcatc
     1261 cttcaggtaa tcctgaacca tcaattgaag caaccgagcg cctatatagt atggcaaaag
     1321 aattagatat agatgtagtt gaccatttaa ttattggtcg tagtagattt tatagttttg
     1381 cttcaaataa gattattgaa gtttaaagat aattgattat tttatattaa tgccttataa
     1441 tgatatggat gtgaggtggt aatttgaata atcgtaaaaa acaaagaaat aagagaatag
     1501 ttttaattat aacggtagca attgttgttt tttctactat atcattggta ttttcaagaa
     1561 cccaaggggg acttgagcga atggtcagcg attcgattgc tgcaatagag tattatgtag
     1621 taaaaaaacc agttgaattt ttaagtaatt tatttagtga atataatgag ttaaaagatg
     1681 tttataaaga aaataagatt ttaaaagcaa aattatcatc ttatgccagt gtcgaagtta
     1741 atactgatgt tctttcaaaa gagattgatg agttgaaaaa gatgctaaat attgaatatt
     1801 tgccaactga ttataatgtt aagacaacat catttgttcg tgaaagtgat gattggaata
     1861 acgaaattac aattgattta ggaagtttgg ctggagttag caaagatatg gtcgtaattt
     1921 cttcaaaagg aatgattggt aaggttactt cggttacaga agtgacggcg cgagttcagt
     1981 tattaacagc agagaatcct acttctgcat taccgattca agttattaat ggtgatcaaa
     2041 atgtttatgg acttttaaat cgttatgaca ttgagtcaaa atgttttgaa attactctat
     2101 ttagtgatgt tgaaaaattt gaagataatg ccaaagtaat aacttctggc ttaggtggga
     2161 aagcgcctaa aggaatttat attggaactg tagaaagttc tattgtttct gaagatggaa
     2221 catcaaaaac aattagagtt aagccggcag cagattttaa tgatctgtca tatgttgcgg
     2281 tagtatttag gagcgatagt aatgaataag caattatatc gttattatct agtgatggca
     2341 ttaagttttg ttgttgatag cattattagt tattatttac cgtataattt tgataaatta
     2401 ggaataacaa ttgttccttg tgttagtttg atgatgttca cattgctgaa taatacaatt
     2461 ggtgatgaac accgatacat atttgcaact gtgactggac tttattatgc gattgtctac
     2521 gcaaatagtt tactgattta tgtgttgctt tattgtgttt atgctttttt tggaaagaaa
     2581 tatactaaat tagcaactta taccctttta gagatgttta tcgccgtaat tgtgacaata
     2641 atcgcccaag aagttgtgat atattggtta gtgtggataa caaatgttac gcagctatca
     2701 atcgtagctt tcttgacaaa tcgtctttta ccgacgttag gagctaattt attattgatt
     2761 gcaccagtat attttatcca caagaaatta ggattcgagg gtaaagtcaa tgcatatcaa
     2821 agtaaaagga gttaataatc atctagtttt tgttttagat gataatcaag attttaatag
     2881 tttaatgaat gaattgg
//
LOCUS       DAOMTA010000349         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1470_length_2888_cov_1.894105, whole genome shotgun sequence.
ACCESSION   DAOMTA010000349 DAOMTA010000000
VERSION     DAOMTA010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_2888_cov_1.894105"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..683)
                     /locus_tag="PLK68_09480"
     CDS             complement(33..683)
                     /locus_tag="PLK68_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HRM90673.1"
                     /translation="MSYNKKDIFLATMLMLFIISLAIVFTVFFKQLYYLDINYLGIDL
                     TSGMSVETIKKNYDVLIAYQSIFYRGTLNLPDFVMSTNGRIHFEEVKTIFEAIQVIMV
                     VSGLISLPLVIRRFKEKEYRFLKLTGLVTIIVPAMLGFVVALDFESAFITFHQIVFRN
                     NYWVFDYRSDPVINILPETFFMHCFIMIVIIVITLAGLCLFYYYHKQKQIINYTIE"
     gene            complement(673..1182)
                     /locus_tag="PLK68_09485"
     CDS             complement(673..1182)
                     /locus_tag="PLK68_09485"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRM90674.1"
                     /translation="MNHIVLVHPAIPQNTGNIMRTCVATNTSLHIIKPMSFELDDKKM
                     KRAGLDYVKDLNLYVYENYEEFEQKNPGEYHFFTRYSHLCYTDQDFSDDKKEHYLFFG
                     HEHDGIPKEILTKHLDRCLRIPMSDKVRSLNLSNCAAICIYEVLRQQSYPNLSKVEVQ
                     KGEDFLYEL"
     gene            complement(1183..1770)
                     /gene="rdgB"
                     /locus_tag="PLK68_09490"
     CDS             complement(1183..1770)
                     /gene="rdgB"
                     /locus_tag="PLK68_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRM90675.1"
                     /translation="MKEIIVASTNQGKIKEIKAMLKDIDIEVLSMKDVLEQELEIEET
                     GTTFKENALIKAQTIANIVNKPVLADDSGLEVDALDKQPGIYSARFLGADTSYNIKNQ
                     YIIDALKDKERTARFVCAMALVIPGQEPILIEETMEGLINDKIEGANGFGYDPIFYFP
                     PCQMTSAMMSMEEKNKYSHRAKALKKLYTILKEIL"
     gene            complement(1770..>2329)
                     /locus_tag="PLK68_09495"
     CDS             complement(1770..>2329)
                     /locus_tag="PLK68_09495"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536062.1"
                     /note="YdiB; quinate/shikimate dehydrogenase from
                     Escherichia coli uses both NAD and NAD(P) to convert
                     quinate and shikimate to 3-dehydroquinate and
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HRM90676.1"
                     /translation="GTITDGIGFTRSLEEQGWLIKDKKITMVGAGGASTAIMVQLALD
                     GVKEIIVYNRTMRTEFQEIINNTIIETGCTITLKSLSDLESLKKDMHSSYLFINTTGV
                     GMEPMLERSVVPDASYFKPDLKVADIIYQPAVTKMLRLAKEAGCATMNGELMLLYQGV
                     ESFKIWTGQEMPINEVKKVLGIEVK"
     assembly_gap    2330..2339
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2340..2649)
                     /locus_tag="PLK68_09500"
     CDS             complement(<2340..2649)
                     /locus_tag="PLK68_09500"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536062.1"
                     /note="YdiB; quinate/shikimate dehydrogenase from
                     Escherichia coli uses both NAD and NAD(P) to convert
                     quinate and shikimate to 3-dehydroquinate and
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HRM90677.1"
                     /translation="MKSQISATTSLYAFIASPAHHSKSPAMHNTAFEQLGLDSVYLAF
                     DIKSEELKDTIAGFKAMKVRGANVSMPHKQNIIPYLDEISTASRLCNAVNTITLKDGK
                     Y"
     gene            2686..>2888
                     /locus_tag="PLK68_09505"
     CDS             2686..>2888
                     /locus_tag="PLK68_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRM90678.1"
                     /translation="MTESLQFLKKIYIIFIIAYNGDIDTEDYEMIKKLIVNADDFGMT
                     EGNSIGILMAHADGILTSTTCMM"
BASE COUNT          947 a          519 c          378 g         1034 t
ORIGIN      
        1 gcctgaatat acgattcatg attatcacga tactattcaa tagtatagtt tattatttgc
       61 ttttgcttat gataataata aaataaacat aaccctgcta gagtaattac aattataaca
      121 atcataataa aacaatgcat aaaaaaggtt tctggtaaaa tattgattac cggatcacta
      181 cgataatcaa atacccagta attgtttcta aatacaattt gatgaaaagt tataaaagca
      241 ctttcaaaat ctaatgctac aacaaaaccc aacattgctg gtacaataat agtaaccagc
      301 ccagtaagct ttaaaaaacg atattctttt tctttaaatc gtctaattac taacggtaat
      361 gagatcaacc cactaactac cataattacc tggattgcct caaaaattgt ttttacttct
      421 tcaaaatgaa tcctaccgtt tgttgacatt acaaaatctg gcagattcaa cgttccccga
      481 taaaagatcg actgatatgc aattaaaaca tcgtaattct tttttattgt ttcaactgac
      541 attccactcg ttaaatcaat tcctaaataa ttaatatcta aataatacag ctgtttaaaa
      601 aagacagtga atactattgc taatgaaata ataaataaca ttaacatcgt tgctaaaaaa
      661 atgtcttttt tattataact catataagaa atcttcccct ttttgaactt caacttttga
      721 aagatttgga taactttgtt gtcttaaaac ctcgtaaata cagatagcag cacaatttga
      781 tagattcaaa cttctaactt tatcactcat cgggattctt aaacaacgat ctaaatgctt
      841 ggttaaaatt tctttaggta ttccatcatg ttcatgacca aaaaataaat aatgttcttt
      901 tttatcatcg ctaaagtctt gatcagtgta acaaagatgt gaataacgcg taaaaaaatg
      961 atattctcca ggattttttt gttcaaactc ttcatagttt tcataaacat acaaatttag
     1021 atctttaaca taatctaatc ccgcacgttt cattttttta tcatctaatt caaaactcat
     1081 tggtttaata atgtgtaaac tagtgttagt agcgacacac gttctcataa tattccctgt
     1141 attttgggga atggctggat gaactaatac gatatgattc atttataata tctcctttaa
     1201 aatcgtatat aattttttta aagctttagc tcgatgtgag tatttatttt tttcctccat
     1261 tgacatcatt gccgaagtca tctgacatgg cggaaaatag aaaattggat cgtatccaaa
     1321 cccattagct ccttcaattt tatcattgat caagccttcc atcgtttctt caattaaaat
     1381 tggttcttga ccaggtataa ctaaagccat cgcacaaaca aagcgagcag tacgctcttt
     1441 atctttaagc gcatcaatta tgtattgatt tttaatatta taactagtat ctgcacctaa
     1501 aaaacgtgct gaataaattc ctggttgttt gtctaaagca tcaacttcta gaccactatc
     1561 gtccgctaag accggcttgt taacaatatt tgcaattgtt tgagctttaa tcagtgcatt
     1621 ctctttaaat gttgttcctg tttcttcaat ttccaattct tgctctaaaa catctttcat
     1681 cgataatact tcaatatcaa tatctttaag cattgcttta atttctttga tttttccttg
     1741 attagttgaa gcaacaatta tttctttcat tattttacct cgattcctag tacttttttt
     1801 acttcattaa ttggcatttc ttgacctgtc catattttaa atgattcaac tccttgataa
     1861 agcagcatca attcaccatt catcgttgca caacccgctt cttttgctaa tcgcagcatt
     1921 tttgtaactg caggctgata aataatatca gctactttta aatctggttt aaaataagaa
     1981 gcatctggta ctactgatcg ttctaacatc ggttccatac cgacaccagt agtattgata
     2041 aaaagataac tgctgtgcat atcttttttc aaactttcta aatcacttaa agattttaag
     2101 gtaatcgtac aacctgtttc aataattgta ttattgatta tctcctgaaa ctctgtacgc
     2161 atagttcggt tataaacgat tatttctttt accccatcta aagcaagctg taccataatt
     2221 gcagttgacg caccgccggc tccgaccata gttatctttt tgtctttaat caaccatcct
     2281 tgctcttcaa gacttctagt aaagccaatt ccatcggtaa tcgtaccatn nnnnnnnnna
     2341 atattttcca tcttttaaag taattgtgtt cactgcatta cataatcttg aagctgttga
     2401 tatttcatca aggtacggaa taatattttg tttatgaggc attgaaacat tagctcctct
     2461 aactttcatc gccttaaacc cagcaatcgt atcttttaat tcctcactct ttatatcaaa
     2521 tgctaaataa acactatcaa ggcctaattg ttcaaaggct gtattatgca tcgctggtga
     2581 tttagaatga tgtgcaggac tagcgataaa agcatataaa cttgttgttg cactaatttg
     2641 tgatttcatg atctccctcc attttttaaa agtattatag catggatgac agaaagtttg
     2701 cagtttttga aaaaaatata tattattttt ataatagcct ataatggaga tatagatacg
     2761 gaggactatg aaatgataaa aaaattaatt gttaatgctg acgattttgg tatgaccgaa
     2821 ggaaattcaa ttggtatttt aatggcacat gctgatggaa ttcttacatc aactacatgt
     2881 atgatgaa
//
LOCUS       DAOMTA010000350         2887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1473_length_2887_cov_2.433616, whole genome shotgun sequence.
ACCESSION   DAOMTA010000350 DAOMTA010000000
VERSION     DAOMTA010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_2887_cov_2.433616"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..>2887)
                     /locus_tag="PLK68_09510"
     CDS             complement(220..>2887)
                     /locus_tag="PLK68_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM90679.1"
                     /translation="TIQIGKVHAVIGGDRNAGDKWLKYSLQYSADGQDWTTYKSYEGV
                     TNGKDVIDENLRGVEARYVRLVNNEQKNSWVIFSEFDVAQFDPIKDYDDTNVYTNTEY
                     RLITESKEDLTKLMYDEVITLTQGQYIGVDLLRIKDISEIVVDMNKDNLTIETSKNAL
                     EWTTVKTKTSELPDARYVRIINNTDSAITFNLNRFEVHSNELYAPSLYETTMGINSSW
                     GVAEDSRNNGAAFDGNIDTTTEFADLPQKGQYIIYDLGQERNISKIQMFCQDSAVNYI
                     RDADILISNDLENWTKVITIGDGVENKNDASVTCINSDAGYKASSTYPNKVLVEGTAD
                     NVKARYLKILMTATNNNRAVLFNEIVINDGEYVPVSNDPTFESNAVEVQGFVPQNMFD
                     GDLTTAYKPNTTDAGYITYTLSDNLDVKKINIVQKGTVSNAKVMALVMVDEEKQWVQV
                     GTLSKSLNEIYLPFDMTYELKIVWEANNIPTISEIVRLNDDEFLPELEALQKYVNSLN
                     VDENNYTASSYAKYVSKLNQANEVLSTASGDKKLIIKAYSELQIAYTNLVTRGNAQLI
                     KDELNKIGALVADDYTDSTWEALQDKVNEANTLLAKGEEELNVKEVADMVNILQVAKS
                     NLITKVSVSKEVLQNYIDTNELDNLDTSKYLTSTATPFVDALKVAHDLIASDDATVKQ
                     LEDALTALQETRAALVLKATADEINAINGLIDSYKENNYTASTWKEFVKVLTEVKDAL
                     NDENSSEDIAELTKTLKAAAEKLVERGDLTDLNILLETAEALDANRYTEESYNKLLDI
                     IKTIKVELKDSSEMLQTDVDKLQAKLQAAIDALEIKPSVTPNEDGIINGNTASDTTTS
                     SKTGDDVVIGSFLVLGMLSIAGLWLYRKKENC"
BASE COUNT          918 a          524 c          325 g         1120 t
ORIGIN      
        1 tcataaacat atccacatac agaacataca aatttagcca ttataattat ctccttttta
       61 tattatttat ttcatcatca cctataatta acagtaatga ttactgatat taatatatca
      121 tattttaatt ttttgtcaat atatatactc acctaaatag aaaaaaagct gaatttttat
      181 tcagccttct tctaaaagag agagagaaat atatcgctat taacaatttt cttttttacg
      241 gtatagccat aatccagcaa tacttaacat tcctaatact aagaatgatc caataactac
      301 atcgtcacca gtttttgaac tagttgttgt atctgatgca gtgtttccat taattatacc
      361 atcttcatta ggggtaactg atggttttat ttctaatgca tcaattgctg cttgcaactt
      421 agcctgtagt ttatccacat cagtttgtag catttctgaa ctatctttta actctacttt
      481 gatagtttta ataatatcca ataatttatt ataagattct tcagtatatc tattagcatc
      541 aagtgcttca gcagtttcta ataaaatatt aagatctgtt aaatctcctc gttctacaag
      601 tttttcagca gcagctttca aagtttttgt taattcagct atatcttcac tgctgttttc
      661 atcatttaaa gcatctttaa cttctgttag tactttaaca aattctttcc atgtagaagc
      721 agtataatta ttttctttat aactatcaat taatccattt atagcattaa tttcatcagc
      781 agttgctttt aaaaccaatg ccgcacgtgt ttcttgtaat gctgttaaag catcttccaa
      841 ttgtttaact gtcgcatcat cactagcaat taggtcatgt gccaccttta atgcatctac
      901 aaatggtgtt gcagtagatg ttagatattt actagtatct aaattatcca actcatttgt
      961 atctatataa ttttgtaata cttctttact cactgaaact tttgtaatta agtttgattt
     1021 agctacttgt aatatgttga ccatatcagc aacttcttta acgttcaatt cttcttcacc
     1081 tttagctaat aaagtattag cctcattaac tttatcttgt aatgcctccc atgttgaatc
     1141 agtataatca tcagcaacta atgcaccaat tttatttaat tcatctttaa ttaattgggc
     1201 attaccacga gtaactaaat tagtataagc aatttgtaat tcactatatg ctttgattat
     1261 taatttctta tctcctgaag cagttgataa aacttcattt gcctgattca atttgctaac
     1321 atacttagca taagaagacg cagtataatt attttcatca acatttaatg agttaacata
     1381 tttttgtaat gcttctaatt ctggtaaaaa ttcatcatca tttaatctta caatttcaga
     1441 aattgttggg atattatttg cttcccatac aatcttcaat tcataagtca tatcaaatgg
     1501 taaatatatc tcatttagtg atttgcttaa agtgcctact tgtacccact gtttttcttc
     1561 atcaaccatt actaaagcca ttaccttagc attacttact gtcccttttt gaacgatatt
     1621 tattttctta acatctaaat tatcagaaag tgtatatgta atataccctg catcagtagt
     1681 atttggttta tatgctgtag ttaaatcacc gtcaaacata ttttgtggta caaatccttg
     1741 tacttccact gcatttgatt caaaagtagg atcatttgat accggaacat actcaccatc
     1801 attaattaca atttcattga ataaaactgc acgattatta tttgttgctg tcattaaaat
     1861 ttttaaataa cgagccttaa cattatcagc agtcccttca accaatacct tattaggata
     1921 agtacttgat gctttataac ctgcatcaga atttatacaa gtaacagagg catcattttt
     1981 attttctact ccatcaccaa ttgttataac ctttgtccaa ttttctaaat cattagaaat
     2041 aagaatatca gcatcccgaa tataatttac tgcagaatct tggcagaaca tttgaatttt
     2101 agaaatattt ctttcttgtc ctaaatcata aataatgtat tgtccttttt gtggcagatc
     2161 tgcaaattct gttgttgtat caatattacc atcaaaagcc gcaccattat tacgactatc
     2221 ttctgctact ccccatgaac tattaattcc cattgttgtt tcatataatg aaggagcgta
     2281 taattcattt gaatgtactt caaatctatt aagattaaaa gtaatggcag aatcagtatt
     2341 attaattatt cttacataac gagcatcagg taattcacta gttttagttt ttacagtagt
     2401 ccactcaagt gcatttttag atgtttctat agtcaaatta tctttattca tatcaacaac
     2461 aatttcacta atatctttaa ttcttaataa atcaacacca atatattgac cttgagttaa
     2521 tgtgattacc tcatcataca tcaatttagt taaatcttct ttactttctg ttatcaatct
     2581 atattctgta tttgtgtata catttgtatc atcataatct ttaataggat caaattgagc
     2641 aacatcaaat tctgagaaaa ttacccaaga atttttctgt tcattattta ctaaacgaac
     2701 atatcttgcc tctactccgc gtaaattctc atcaataaca tctttgccgt tagtaacacc
     2761 ttcatatgat ttatatgttg tccaatcttg accatcagca gagtattgta aactgtattt
     2821 aagccacttg tcacccgcat tacgatctcc accaataaca gcatgaactt ttcctatctg
     2881 aattgtt
//
LOCUS       DAOMTA010000351         2887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1475_length_2887_cov_1.897599, whole genome shotgun sequence.
ACCESSION   DAOMTA010000351 DAOMTA010000000
VERSION     DAOMTA010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1475_length_2887_cov_1.897599"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..683
                     /locus_tag="PLK68_09515"
     CDS             51..683
                     /locus_tag="PLK68_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90680.1"
                     /translation="MRRKENTEVDILYNLLTYINASYSQDMYYTICYQVLNNIEKIPD
                     ISINELADLCYTSPATISRFCKALKCDNFAEFKKEVQVGLKQASHEIKLHPDDLVEIH
                     QNPVHCVDMVYDLTINSLIESKKHINIHEIDRLCDIIYDAKKLHFFGFQFNKILASDI
                     QFKLVKLGKFSYAFADRGDDSQRIELLDEDSVAIVLSVRARLSPVGDLIN"
     gene            888..1916
                     /locus_tag="PLK68_09520"
     CDS             888..1916
                     /locus_tag="PLK68_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM90681.1"
                     /translation="MYSFTNDYSEGAHPKIMKDLIETNDEQCAGYGLDKYCLEATRLL
                     KRQLQNDNVDIHYLVGGTQTNAVFISSVLRPYQAVIAADSGHINVHETGAIEATGHKV
                     LTRPHQDGKLTVAMIESIIEEHSDEHMVQPKMVYISQSTEYGSVYSLDELKDIATLCK
                     KKELYLFVDGARLGSALALPDTPTFKDLADYSDAFYIGGTKMGALFGECLVIVNDSLK
                     ADFRYNMKQKGAMMAKGRLLGVQFKELFSNDLYLEIGKYENKMADILRAGLKDFEMMV
                     PSQTNQVFPIMDDELIAKLQQDFSFNIMDRIDDHRHCIRLVTSWATPKEAVNSFVSKL
                     SELLHNNL"
     gene            1984..2240
                     /locus_tag="PLK68_09525"
                     /pseudo
     CDS             1984..2240
                     /locus_tag="PLK68_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            2253..2666
                     /locus_tag="PLK68_09530"
     CDS             2253..2666
                     /locus_tag="PLK68_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRM90682.1"
                     /translation="MTFGSFLIGYERQNHLKVAGVRTHLIVCLAAAMIMIVSKYGFND
                     ILSHEGIAPDPSRSAAQIISGVSFLGAGIIFVHKREITGFTAAEIWAAAAVEMAIVAG
                     MYVIGILATILILLVQIIFHKQYRWIPTVWESWLI"
BASE COUNT         1027 a          362 c          564 g          934 t
ORIGIN      
        1 ttctttttca ataaattgtg tataatgagt gagagagaag agggatgaac atgagacgta
       61 aagaaaatac tgaagttgat atcttgtata atttattgac atatattaat gcttcataca
      121 gccaagatat gtattatacg atctgttatc aagtattaaa taatattgaa aagattccag
      181 atatctcaat caatgagtta gcggacttat gttatacttc gcctgcaacc atttcccgtt
      241 tttgcaaagc tttaaaatgt gataattttg ctgaatttaa aaaggaagta caagtaggat
      301 taaaacaggc aagtcatgag attaaactcc atcctgatga tttagtagag attcatcaaa
      361 atcctgtaca ctgtgttgat atggtctatg atctaacaat taattcatta attgagagta
      421 agaaacatat taatattcat gagattgatc gcctatgtga tattatttat gatgcgaaaa
      481 agttacattt ttttggtttc caatttaata agattttagc ctcagatatt caatttaaat
      541 tagttaagct aggtaaattt tcatacgcct ttgctgatcg tggtgacgat agtcaaagaa
      601 ttgaactttt agatgaggat agtgtggcta ttgtactttc agttcgagcg cgattatcac
      661 cggttgggga tttaatcaat taaaaatcgt gaagctaaag taattttagt tactttaaat
      721 gctgaaagtg aagtaattga attagctgat aaaacttttg ttgttcatgg taaagaaagt
      781 gattttacag agtcatcaat ttctggaacg acagtgatga aaacgttttt tgatgtattg
      841 tatgtaagat atggattatt atatccaaga agataagggg gcaggttatg tatagtttta
      901 ctaatgatta tagtgaaggt gctcatccaa aaataatgaa agacttaatt gaaacaaatg
      961 atgagcaatg tgctggttat ggattagata aatattgtct agaggctaca agattgttaa
     1021 aaagacaact tcaaaatgat aatgttgata tacactattt agttggtgga acacaaacta
     1081 atgcggtatt tatttcatca gtattacgtc catatcaggc agtgattgca gcagatagcg
     1141 ggcatattaa cgttcatgaa accggagcaa ttgaagctac gggacataaa gttttaacta
     1201 gacctcacca agatggtaaa ttaacagtag cgatgattga aagtattatc gaagagcatt
     1261 cggatgaaca tatggtgcaa ccaaaaatgg tatatatttc acaatcaaca gaatatggtt
     1321 ctgtttatag tcttgatgaa ttaaaggata tagctacttt atgtaagaaa aaagaattgt
     1381 atttatttgt tgatggagct cgattaggta gtgccttggc gctacccgat acacctacat
     1441 ttaaagattt agcggattac agcgatgctt tctatattgg aggaacaaaa atgggagcat
     1501 tattcggtga atgtctagta attgttaatg atagtctgaa agcggatttt agatataata
     1561 tgaaacaaaa gggggcgatg atggctaaag gtcgactttt aggagtccaa tttaaagaac
     1621 tttttagtaa tgatctatat ttagaaattg gaaaatatga aaataagatg gctgatattt
     1681 tacgagctgg tctaaaagat tttgaaatga tggtaccttc acaaactaat caagtatttc
     1741 cgataatgga tgatgagttg attgctaaat tacaacaaga ttttagcttt aatatcatgg
     1801 atcgcattga tgatcaccgc cattgtattc gtcttgttac ttcgtgggca acccctaaag
     1861 aagctgttaa ttcgttcgtt agtaaattaa gcgaactact acataataac ttgtaataaa
     1921 tattaataag taatataata ttcgtaggat tattcctata ctatatagaa tggagaaatt
     1981 aaaatgaaca aaactgaatt agtagaagca attgcttcaa gtacagaaat gacaaaaact
     2041 gatgttgata aagtagtaac tgcatttgta gacgtagtta ctgaagctct ggtaaaagga
     2101 gataaagtat ctcttaaagg atttggtaac tttgaagtac gtgaaagagg agaaagaact
     2161 ggacgtaacc caagaactgg tgaaacaatg acaatgcatt caaatctagt agtgcattaa
     2221 aaaatgccgt aaataaataa tataatataa aaatgacctt cgggtcattt ttaattgggt
     2281 atgaacgtca aaatcatctt aaagtagcag gggtaagaac tcatttgatt gtctgtttag
     2341 cggctgctat gataatgatt gtttctaaat acggttttaa tgatatttta agccacgaag
     2401 gaatagcacc tgacccatca cggagtgcag cacaaattat ttctggagta agttttttag
     2461 gagctggtat aatttttgtg cataaacgag aaataaccgg ttttacagca gcagaaattt
     2521 gggcagctgc ggcagtagaa atggcaattg tggcgggaat gtatgtgatt ggaatcttag
     2581 ccactatttt aattttgtta gttcaaatta tttttcacaa acaatatcgt tggattccca
     2641 cagtatggga gagttggtta atttagggta aaggataaat ctgatgtaat ttccaaaatt
     2701 caaaaaatta tgcatgagta tcatattgag ataatgaatt ttaaaattag tcataatcaa
     2761 agtaccataa tactagaatt attagtcaag ttgccgacca gtttccccct agaagatatg
     2821 ttgactttat ttgataaaaa taatgatatt atttcaatcg agataaaata aaaagcctat
     2881 tataatt
//
LOCUS       DAOMTA010000352         2882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1480_length_2882_cov_2.707817, whole genome shotgun sequence.
ACCESSION   DAOMTA010000352 DAOMTA010000000
VERSION     DAOMTA010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_2882_cov_2.707817"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLK68_09535"
     CDS             <1..956
                     /locus_tag="PLK68_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRM90683.1"
                     /translation="GVDTKAKVAVGVVTALVIAGIAFAIYLGVKPDTSLMPDLTEKTV
                     EQAKEILKEYNVTISDDITEELSDEYEKGEIIATDPKKGTTIKEGDVVKVTVSKGKYI
                     VLEDYTGKKEDVAKKALEKLKFEVEIEYEISSKTKGTVIDQSIEKGTKVDPTEKDRKI
                     ILTVSKGDYVVLGNYVGMDQNKAKEALTKLGFDVTIKETSSEQAVGTVVEQSLKEGHK
                     VDPDEKDRTITLTVSSGIKIEVPNVKGMDIDAAATLLTNKGFSVKRETLPTPTDPNEI
                     EKITVNQVVRQSLDAFTTVTKKNESITLYYYNYKPEIPTDD"
     gene            966..1841
                     /gene="rsgA"
                     /locus_tag="PLK68_09540"
     CDS             966..1841
                     /gene="rsgA"
                     /locus_tag="PLK68_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRM90684.1"
                     /translation="MSQGRIIKALAGFYYVEDDHQIIQCRARGKFRKDEIKPLVGDFV
                     EYEVEGDNDGYVMNVLPRHNCLVRPPICNVDQALIVSSCKEPDFSSILLDKFLLVIEH
                     LGIEPIIIISKMDLDEDESVKQYVKDYRQAGYRVYEISSKDNHGLEELKTVFKDKVSV
                     ITGQSGVGKSSFLNALDINLKLETNEISKSLGRGKHTTRHVELLKMYGGYLGDTPGFS
                     SLELEMTPEELAVAYHDFAQFSHECKFRGCLHDSEPNCGVKKAVEDGKISKERYEHYL
                     MNLQDVKKKEERKYG"
     gene            1834..2475
                     /gene="rpe"
                     /locus_tag="PLK68_09545"
     CDS             1834..2475
                     /gene="rpe"
                     /locus_tag="PLK68_09545"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM90685.1"
                     /translation="MVKVAPSLLSANFAYLKEEINAIKAADWIHYDVMDGHFVPNISF
                     GYSILKDVSKVTDMYLDVHLMITDPAKYVDNFINSNASLIVFHYEAVAEDKINELIAH
                     IKEHDVEVGLSIKPDTPVEVLKPFLNELDVVLVMSVEPGFGGQKFNSAAVDKIAQLAT
                     LRKENNYHYLIEVDGGINESTSKLCNDAGVDVLVAGSYVFGSDDYTKAIESLK"
     gene            2472..>2882
                     /locus_tag="PLK68_09550"
     CDS             2472..>2882
                     /locus_tag="PLK68_09550"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRM90686.1"
                     /translation="MKIGICSALACKLDDSLKYIGVDHGVEILLKQGIKPVFAIGDFD
                     SIENENVLSELDIERLPTRKDVTDTHAALEYALENGYDEVDIYGVTGGRLDHFLSVMC
                     LLEKYADKKIRIIDQQNVIQLLLPGTHKVYQDEYK"
BASE COUNT         1038 a          338 c          616 g          890 t
ORIGIN      
        1 aaggtgtaga tacaaaagct aaggtggcag ttggtgtggt tactgcttta gttatcgcgg
       61 gaattgcttt tgcgatttat cttggagtga agccagatac tagtctaatg cctgatttaa
      121 cagaaaaaac agttgaacaa gcaaaagaaa ttttaaaaga atataatgtt actattagcg
      181 atgatattac tgaagaacta tctgatgaat atgaaaaagg tgaaatcatt gcaacagacc
      241 ctaaaaaagg aacgacgatc aaagaaggtg atgtcgttaa agtaacagta tcgaaaggca
      301 aatatattgt tcttgaagat tacactggaa aaaaagaaga tgttgctaaa aaggcattag
      361 aaaaattgaa atttgaagta gagattgaat atgagatatc ttctaaaact aaaggaactg
      421 taattgatca aagtattgaa aaaggaacta aagtagatcc aactgaaaaa gatcgtaaaa
      481 ttattttaac agtatcaaaa ggtgattatg tagttcttgg taattatgta ggaatggatc
      541 aaaataaggc caaagaagca ctgacaaaac ttgggtttga tgttacgatc aaagaaacta
      601 gttcagaaca agcagttgga acagttgttg aacagagtct aaaagaaggg cataaggttg
      661 atcctgatga aaaagatcgg acaattacat tgactgtttc ttcgggaatt aaaattgaag
      721 taccaaatgt taaaggaatg gatattgatg ctgctgcgac cttactaaca aataaaggtt
      781 tttcagttaa acgggaaaca ttgccaacac ctacagatcc aaatgaaatt gaaaagatta
      841 ctgtaaatca agtagtccgt caaagtttag atgcctttac tacggtaact aagaagaatg
      901 aatctattac actttattat tataattata aacctgaaat acctacagat gattaggagg
      961 tagagatgtc acaaggaaga attattaaag cattagcagg gttttattat gtcgaagatg
     1021 atcatcaaat aattcaatgt cgtgctaggg ggaaatttcg taaggatgag attaagcccc
     1081 tggttggtga ctttgttgaa tatgaagtcg aaggtgataa tgatggctat gtaatgaatg
     1141 tattgcctcg tcataattgt ttagttcgac cacctatttg taatgttgat caagctctga
     1201 ttgttagttc ttgtaaggaa cctgattttt catcaatatt attagataaa tttcttttag
     1261 taattgagca tttaggaatt gaaccaatta ttattatttc taaaatggat ttagatgaag
     1321 atgaaagcgt taaacaatat gttaaagatt atcgtcaggc tggatatcgg gtatatgaaa
     1381 taagctcaaa agataatcat gggcttgagg aattaaaaac agtctttaaa gataaagtat
     1441 cagtgattac tggacaaagt ggggtaggca aaagtagttt tttgaatgcc ttagatatta
     1501 atttaaaatt ggaaacaaat gagatatcaa aatctcttgg gcgaggaaaa catacaactc
     1561 gtcatgttga actgctcaag atgtatggtg gatatcttgg agatacacct ggtttttctt
     1621 cattagaatt ggaaatgaca ccggaagaac tagctgtagc ctatcatgat tttgctcaat
     1681 ttagtcatga atgcaagttt agaggatgtt tacacgatag tgaacctaat tgtggggtaa
     1741 aaaaagccgt tgaagatgga aaaatatcta aagaacgcta tgagcattat ttaatgaatt
     1801 tacaagatgt aaaaaagaaa gaggaacgga agtatggtta aagtagcacc ttcattatta
     1861 tcagctaatt ttgcttattt gaaagaagag attaacgcaa tcaaagcggc agattggatt
     1921 cattatgatg taatggatgg acattttgtt ccaaacatat catttggata tagtatttta
     1981 aaagatgtta gtaaagtaac ggatatgtat cttgatgtac atttaatgat tactgatcca
     2041 gctaaatatg tggataattt tattaatagt aatgcgtcat taattgtttt tcattatgaa
     2101 gcagttgctg aagataagat taatgaatta atcgctcata ttaaggagca tgatgtggag
     2161 gtcgggctat caattaagcc ggatacacca gtagaagtac taaaaccatt tttgaatgag
     2221 ttagatgttg ttttagtaat gtcagttgag cccggttttg gtgggcaaaa atttaattcg
     2281 gcagctgttg ataaaattgc acagctggca acactcagaa aagaaaataa ttatcattat
     2341 ttaattgaag tagatggagg aatcaatgaa tcgacttcca agttatgtaa tgatgctgga
     2401 gttgacgttt tagtggctgg cagctatgtt tttggtagtg atgattatac gaaagcaata
     2461 gagtcactaa aatgaagata ggtatttgta gtgcactggc ttgtaaactc gatgacagtt
     2521 taaaatatat tggtgttgat catggtgttg aaatattact taaacagggg attaagcctg
     2581 tctttgcaat tggtgatttt gattcgattg aaaacgagaa tgttttaagt gagttagata
     2641 tagaaagatt gccaactcgt aaagatgtga ccgatacaca tgcagcttta gaatatgctt
     2701 tggagaatgg ttatgatgaa gtagatattt atggtgtgac aggcggtcgc ttagatcatt
     2761 ttcttagcgt tatgtgtctt ttggaaaaat atgcagataa aaaaattaga attattgatc
     2821 agcaaaatgt tattcaatta ttattgccgg gaacacataa ggtgtatcaa gatgaatata
     2881 ag
//
LOCUS       DAOMTA010000353         2880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1481_length_2880_cov_2.797876, whole genome shotgun sequence.
ACCESSION   DAOMTA010000353 DAOMTA010000000
VERSION     DAOMTA010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1481_length_2880_cov_2.797876"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLK68_09555"
     CDS             <1..189
                     /locus_tag="PLK68_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRM90687.1"
                     /translation="NLAYGYLWWLHQDGFMAMGDGGNVIYVNIMKKIVISITATFKPD
                     VNDRIEFIKEYLEPLFDE"
     gene            244..657
                     /locus_tag="PLK68_09560"
     CDS             244..657
                     /locus_tag="PLK68_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HRM90688.1"
                     /translation="MDKIKIRGLIQNNLKNIDLDIPKHKIVIFTGVSGPGKSSIVFDT
                     IATESGRQLNETFAAFIRGKLPQYAKPNLQSIENLSPAVIIDQSSLGGNIRSTAGTIS
                     DLYTDLRILFSRIGKPYFGSAACFSFNDPAQYVRD"
     gene            666..854
                     /locus_tag="PLK68_09565"
     CDS             666..854
                     /locus_tag="PLK68_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90689.1"
                     /translation="MTIEEVLDFFDNAKITKKLQQLVDVGLSYMTLGQSLTALSGGEI
                     QRIKLAQALNKKGNIYIR"
     gene            895..1068
                     /locus_tag="PLK68_09570"
     CDS             895..1068
                     /locus_tag="PLK68_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRM90690.1"
                     /translation="MKLFNSLVDSGNTVIIIEHNLDVIKQADWIIDIGPEGGKNGGKV
                     VFQGTPKEMITTS"
     gene            1315..>2880
                     /locus_tag="PLK68_09575"
     CDS             1315..>2880
                     /locus_tag="PLK68_09575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM90691.1"
                     /translation="MEYQDRLFNQKNRASMDKIINNLPGGIIVFEMQENKIKTLFSND
                     GTYQLFGYSKEAFKTVFHSDVFEIVYQPDLAPLQEEFYEATKNRESFEHGCRCFRNDG
                     TLMWTYFRVNYIGTEDGRNVFVGLITDSSKERLLEEKSRINEEMYHIAFNQSNVFLLE
                     YDHRSKQVSCSSESSAAYFDSAAFSNIPESLIDSDLIHERSFDDFYHFYHQIIGGVKT
                     GEVKLKMRALNSDKYIWIHVKFTNIFDDDNQPIKAVGVYQNIDEQVRLESRYLQEIKY
                     RQKLMQRSITNLEVNLTTDEVIKAHNVTYNLLGLDEKASYSEFIKKMVTLVADEDKHL
                     FLDTFGLDNVRQAYHDGKDDLRISFLFYRPTKKRYGWVSTHMMFLYNEETNQLMGFFY
                     ANDIDDDKRKTIELTRQARTDALTGLINRRELERVITSEIQLVEPGNYGALFMIDVDN
                     FKLVNDANGHSAGDETLRFVANRLKSLFRGDDVVGRFGGDEFMVYMKEVGAKKDVISK
                     AKQVSEALATTCPG"
BASE COUNT          967 a          353 c          584 g          976 t
ORIGIN      
        1 aatttagcat atggatattt atggtggctg catcaagatg gttttatggc aatgggtgat
       61 gggggaaatg ttatttatgt aaatatcatg aaaaaaatag taatatcgat tactgctacg
      121 tttaaaccag atgttaatga tcgaattgaa tttattaaag aatatctaga accgctattt
      181 gatgaataat taaataagaa cagataactt taaagttatc tgttctttaa ataggggaat
      241 ataatggaca aaataaaaat tagaggtttg attcaaaata atcttaaaaa tattgattta
      301 gatattccca aacataaaat tgtaatcttt accggggtat cagggccggg aaagtctagt
      361 attgtttttg atacgattgc cacagagtca ggtcgtcagc taaatgaaac ttttgcggct
      421 tttataaggg gaaaattgcc acaatatgct aaaccaaatc ttcaaagtat tgaaaatttg
      481 tcaccagcgg ttattatcga tcaatcttct ttgggtggga atattcgttc aacagctgga
      541 acaattagtg atttgtatac tgatttacgc attttatttt ctcgtattgg taagccttat
      601 tttggcagtg ccgcgtgttt ttcttttaat gatcccgccc agtatgttcg ggattaggag
      661 aagtaatgac aattgaggag gttcttgact tctttgataa tgctaaaatt actaagaagc
      721 ttcaacaatt agtcgatgtg ggattatcgt atatgacctt aggtcagtct ttaacagctt
      781 tatctggtgg tgagatccaa aggatcaagt tagctcaagc tttgaataaa aaagggaata
      841 tatatattag atgagccgac gacaggattg cacccgtgcg atataacgaa attgatgaaa
      901 ttatttaatt cgcttgtcga tagcggtaat acagttatta taattgaaca taatcttgat
      961 gtaattaaac aggctgactg gatcattgat attggtcctg aaggtggtaa aaatggaggc
     1021 aaagttgtat ttcagggaac ccccaaggaa atgattacaa cttcttagtc ccttacagca
     1081 aagaatatac gggaaacgac ggagttatta gagaaaaaat aaactgttct taagatagtt
     1141 tatttttatt ttaaattttt gtattaagat ataattttag ggtttccttt ttttaggtga
     1201 ataatgtaaa ttatttggtt attatcagac tttttgtcga taatctcaca attgaagaag
     1261 tgatattatt gtgatataat tttcaagtat cttacaaaga atggaggtct ggaaatggaa
     1321 tatcaagata gactgtttaa tcagaaaaat agagcatcta tggataaaat cattaataat
     1381 ttaccaggtg ggattattgt ttttgaaatg caggaaaata agattaaaac attattttca
     1441 aatgacggaa cttatcagtt atttggatat agtaaagaag cttttaaaac ggttttccat
     1501 agtgatgttt ttgaaattgt ttatcaacct gatctagcac ctctacaaga agaattttat
     1561 gaagctacta agaatagaga atcatttgag catggctgcc gttgttttcg taatgatggg
     1621 acattgatgt ggacatattt tagagtgaac tatattggca ctgaagatgg ccgaaatgtg
     1681 tttgttggat tgattaccga tagttctaaa gaacgcctgc tagaagaaaa atcgcgaatt
     1741 aatgaggaaa tgtatcatat tgcatttaat caaagtaatg tatttttatt agaatatgat
     1801 catcgtagta aacaggtttc ttgttcaagt gaaagtagtg cagcgtattt tgattcagct
     1861 gctttttcaa atatcccaga atcattgatt gatagtgatt tgattcacga acgttcattt
     1921 gatgattttt atcattttta tcatcagatt attggtggcg taaaaacagg ggaagttaaa
     1981 ttaaaaatgc gtgcacttaa tagtgataaa tatatttgga ttcatgttaa attcactaat
     2041 atttttgatg atgacaatca gccgatcaaa gccgttggag tttaccaaaa tattgatgag
     2101 caagtgcgac ttgagtcacg ttatcttcaa gaaattaaat atcgtcaaaa attaatgcaa
     2161 cgctcaatta caaatcttga agttaattta actactgatg aagtgattaa agcacacaat
     2221 gttacatata atcttttagg attagatgaa aaagcaagtt atagtgaatt tattaaaaag
     2281 atggttacat tagttgctga tgaagataaa cacttatttt tagatacttt tggattggat
     2341 aatgtacgtc aagcttacca tgatggtaaa gatgatctga gaattagttt tttattttat
     2401 cgtccaacga aaaagcgtta tggttgggta tctacgcata tgatgttttt atataatgaa
     2461 gaaactaatc agttgatggg ctttttttat gctaacgata ttgatgatga taaacgtaag
     2521 acgattgaat tgacacggca ggcgcggact gatgctttaa ccggattaat taatcgtcgt
     2581 gaattggaac gggtaattac atcagagatt cagttagttg aaccaggtaa ctatggagcc
     2641 ttatttatga ttgatgtaga taattttaaa ttagttaatg atgcaaatgg acatagtgct
     2701 ggtgatgaaa cgttacgttt tgttgctaat cgattaaagt cattattccg tggtgatgat
     2761 gttgtcggtc gttttggggg cgatgaattc atggtctata tgaaagaagt aggagctaaa
     2821 aaagatgtta tttctaaggc aaaacaggtt agtgaggcac tagcaacgac ttgtccagga
//
LOCUS       DAOMTA010000354         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1494_length_2862_cov_1.981831, whole genome shotgun sequence.
ACCESSION   DAOMTA010000354 DAOMTA010000000
VERSION     DAOMTA010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1494_length_2862_cov_1.981831"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..565)
                     /locus_tag="PLK68_09580"
     CDS             complement(128..565)
                     /locus_tag="PLK68_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90692.1"
                     /translation="MFRGLFKKHEEDEDIFEVDPVVEQRRKEKFSTPLIYDEEFKEEP
                     EVTVTPTPKTKEKTKVVEAKPKVEEKPRQIYQMSEVISPMMGITKGKNDKKAVKPTTS
                     SKPKKRKNVDQLVPVISPFFGNYEEKEQVESEAKLTTINDEPK"
     gene            complement(583..1182)
                     /locus_tag="PLK68_09585"
     CDS             complement(583..1182)
                     /locus_tag="PLK68_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HRM90693.1"
                     /translation="MIFHGFYNLKHVGDILLARCGEGRTFDYDKYDDLVVLKDSKNRI
                     LGFNLLNASNYLGELNTGLVNFSDNQIEKFNELLTTHGLDPVVLDKEPRFIVGEVTAM
                     EEHPDSDHLHICQVDLKNTTTQIVCGAPNVEVGQRVVVATIGAVMPSGLVIKPSKLRK
                     IDSNGMICSARELGLPNAPQIRGILVLDKDKYSIGDSFF"
     gene            complement(1194..1514)
                     /locus_tag="PLK68_09590"
     CDS             complement(1194..1514)
                     /locus_tag="PLK68_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM90694.1"
                     /translation="MDKLVEIKTIEQFDNIRVGKTIIMFTADWCPDCMFVKPFIGDIV
                     DANPEYKFYMINRDNMIDLCKDLDILGIPSFVAYNDKEESGRFVSKLRKTKEEIQAFI
                     DTLK"
     gene            complement(1507..2160)
                     /gene="trmB"
                     /locus_tag="PLK68_09595"
     CDS             complement(1507..2160)
                     /gene="trmB"
                     /locus_tag="PLK68_09595"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRM90695.1"
                     /translation="MRLRNNPKAYDIMKENKSFVILEPENCKNKWKDIFGNNHPIYIE
                     IGMGKGDFIYENARQFPEINFVGIEKYPSVLAAAINKINAREEKVNNLRLMHYDAIEL
                     HQVFEKDEVDKIFLNFSDPWPKSKHAKRRLTSSKFLDVYKDILIDEGNIEFKSDNRGL
                     FEYSIISLNQYPMDIEYISLDLHNSPESEINIMTEYERKFCEKGPIYKLVARYRKNG"
     gene            complement(2193..>2862)
                     /locus_tag="PLK68_09600"
     CDS             complement(2193..>2862)
                     /locus_tag="PLK68_09600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM90696.1"
                     /translation="KEVKSLIRKGFVKVNGMIIKKDDFKVDHEIDEIVFDDELIEYRP
                     YVYIMLNKPAGYISATKDNLHPTVLELIEGYENYDLFPVGRLDLDTEGLLLITNDGDF
                     SHKLLAPSRNHSKIYFANIAGVMDEQDIQAFKDGIILNTGYHCKPANLEIINKNNDSC
                     DVRIEIFEGKFHQVKKMVEVCGKEVIYLKRLSIRNLELDRSLALGDFRELSNEELVDL
                     MEDL"
BASE COUNT          902 a          576 c          312 g         1072 t
ORIGIN      
        1 caaaatctaa tttgaactca ccagttcgct cctcaataat tttaagctga tcctgttctt
       61 cttccacaat tttagctata ttacgtaaat tatcttctac tgtaggcaaa tcttcaataa
      121 ctttttttta tttaggttca tcattaatag ttgtcagttt tgcttctgat tcaacttgtt
      181 ccttttcctc ataatttcca aaaaaaggac taattacagg aactaattga tcaacatttt
      241 tacgtttttt aggttttgaa gaagttgttg gctttacagc ttttttatca ttttttcctt
      301 ttgtaattcc catcattggt gaaataactt cactcatttg ataaatttga cgaggttttt
      361 cttctacctt aggctttgcc tcaacaacct tagttttttc tttagtcttt ggggtaggcg
      421 ttacagtcac ttctggctcc tctttaaatt cttcatcata tattaatggc gtgctaaatt
      481 tttccttacg tcgctgttca acgacaggat ccacttcaaa aatatcttca tcttcttcat
      541 gctttttaaa taagcctcta aacatcactg tttccccctt ttctagaaaa atgaatctcc
      601 aatactatat ttatctttat ctaaaactaa tatacctcta atttggggag cattaggcag
      661 tcctaactct cttgctgaac aaatcatccc gttagaatca attttacgta gttttgaagg
      721 tttaataacc aatccactag gcataactgc gccaatagta gctactacca ctctttgccc
      781 aacttcaaca ttaggggcac cacatacaat ttgtgtagta gtattcttta aatcaacctg
      841 acaaatatgt aagtggtcag aatctggatg ttcttccatt gcagttactt caccaacaat
      901 aaaacgaggt tctttgtcta agacaaccgg atcaagtccg tgagttgtta aaagttcatt
      961 aaatttttca atttgattat ctgaaaaatt aactagtcca gtatttaatt ctcccaaata
     1021 attactagca ttcaacaaat taaaaccaag tatcctattt ttactatctt tcaatacgac
     1081 taaatcatca tatttatcat aatcaaaggt cctaccttct ccacatcgtg ctaataaaat
     1141 atcaccaaca tgttttaaat tataaaatcc atgaaatatc attcctacac ctcttatttt
     1201 aacgtatcaa taaacgcctg tatttcttct ttagtcttac gtaacttact tacaaagcgt
     1261 ccactttctt ctttatcgtt ataagctaca aaagaaggaa tacctaaaat atccagatcc
     1321 ttacacaaat caatcatatt gtcacgatta atcatataaa acttgtattc aggattggca
     1381 tcaacaatat cgccaatgaa cggttttaca aacatacagt caggacacca gtctgctgta
     1441 aacattataa tcgttttacc tacacgaata ttgtcaaatt gttcgattgt tttaatttct
     1501 actaatttat ccatttttac gataccttgc taccaattta taaattggcc ctttctcaca
     1561 aaatttccgc tcatactcag tcataatatt gatttcactt tcaggactgt tatgcaaatc
     1621 taaactaatg tactctatat ccattggata ttggtttaaa gaaataatcg aatattcgaa
     1681 cagtccacga ttatcacttt taaattcaat atttccttca tctattaaaa tatccttgta
     1741 tacatctaaa aatttacttg atgtaagtcg tcttttagca tgcttacttt taggccaagg
     1801 atcactaaaa ttcaagaata ttttatcaac ttcatctttt tcaaatactt gatgcaattc
     1861 aattgcatcg taatgcatta aacgcaaatt atttactttt tcttcacgag catttatctt
     1921 atttattgct gctgctaaaa cacttgggta tttttcaata ccaacaaaat ttatttctgg
     1981 aaattgacga gcattttcat aaataaaatc acctttaccc atcccgattt caatatatat
     2041 tggatgatta tttccaaaaa tatctttcca tttattttta caattttccg gctccaatat
     2101 tacaaaagat ttattttctt tcataatatc atatgctttt ggattatttc ttaaacgcat
     2161 tattactccc ctttacatat aaaaatattc tatcataaat cctccatcaa gtcgactaat
     2221 tcctcattag ataattctcg aaaatcaccc aatgctaaac tcctatccag ttcaagattg
     2281 cggatcgata agcgttttaa atatattacc tctttaccac aaacctcaac catctttttt
     2341 acttgatgaa actttccttc aaatatttca attctaacat cacaactatc attgttttta
     2401 ttaataattt ctaggttggc aggtttacag tgatagccag tatttaaaat aatcccatct
     2461 ttaaatgcct gaatatcttg ttcatccata actcccgcaa tatttgcaaa atatattttt
     2521 gaatgatttc gactaggggc taataattta tgactaaaat caccatcatt agtaattaac
     2581 aatagtcctt ctgtatctaa atccaagcgg ccgacaggaa ataaatcata attttcatag
     2641 ccctcaatca gttctaatac tgtaggatga agattgtctt tagttgcact aatatatccc
     2701 gctggcttat tcagcattat atagacatat ggccggtatt caatcaactc atcatcaaat
     2761 actatttcat caatttcatg atcaacttta aaatcatctt tctttataat cattccattt
     2821 acttttacaa aaccttttcg aatcagtgat tttacttctt tt
//
LOCUS       DAOMTA010000355         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1495_length_2861_cov_2.867783, whole genome shotgun sequence.
ACCESSION   DAOMTA010000355 DAOMTA010000000
VERSION     DAOMTA010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_2861_cov_2.867783"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLK68_09605"
     CDS             <1..493
                     /locus_tag="PLK68_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRM90697.1"
                     /translation="AKDNKSLGKFKLKKIKRAMRGVPQIEVTFDIDANGIVNVSAKDL
                     ASGNMQSITIENTSNMSDDDIERAIHEAKQYEQQDAVTKERLVFKNEVENLILTVEAA
                     LSKNGKQIDKAMKSNIKNELASFKKLTKKVDFETCDQIQFDEIKTAKLNLETVAASLL
                     SMQ"
     gene            531..1079
                     /locus_tag="PLK68_09610"
     CDS             531..1079
                     /locus_tag="PLK68_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRM90698.1"
                     /translation="MIDEILAYNRAFVTNKGYKPYTTSKYPDRKLAIVTCMDTRLIEL
                     LPAALGIKNGDAKIIKNAGGVIVHPFGSAVRSLLIAIYELNVEEIMIIGHTDCGVGSI
                     DIEAMLKKMEKRGISETVIRDLGYCGIDFNKWLGGFDDVEISVKESVSLLRRHPLLPK
                     NIEIHGLVMDSRTGALSLVEKN"
     gene            1291..2202
                     /gene="nadA"
                     /locus_tag="PLK68_09615"
     CDS             1291..2202
                     /gene="nadA"
                     /locus_tag="PLK68_09615"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRM90699.1"
                     /translation="MNDIIEEIQKLKKEKNAVILAHYYVDGAIQDIADYLGDSYYLSK
                     IAVDCPQDVIVFAGVEFMGESAKLLSPNKTVLMPDKDADCPMAHMVDEYFIARLRKEY
                     EDLCVVCYINSTAEIKTMSDVCVTSSNAKRIVEALPNKNILFIPDQNLGKHIAELVPS
                     KNFLFCNGYCPTHYRITPEDILTAKEKHPGAPVLIHPECKPECVELADYAGSTSGIID
                     YATNDQEHDEFIVVTEIGVIHEMEKRNPGKKFYPATDKLVCPNMKKNTLEKVRDCLKN
                     NTNQVELDEEFMEKAKKPLERMHELAK"
     gene            2216..>2861
                     /locus_tag="PLK68_09620"
     CDS             2216..>2861
                     /locus_tag="PLK68_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRM90700.1"
                     /translation="MNNNYDVIIVGTGAAGLFAGLCLPADLKVLMITKDKVENSDSYL
                     AQGGICTLKSADDFDAFYQDTLKAGRNENNPESVKIMIQQAPQIMKDLMDYGVEFDRD
                     TEGNLAYTREGAHSEYRILHHQDVTGKEITSKLIKQVEMRNNITMVEDATMLDIINHD
                     NIATGIVMENNGEIIQINAKVIILATGGIGGLFTHSSNFRHITGDSFAIALRNNI"
BASE COUNT         1031 a          346 c          576 g          908 t
ORIGIN      
        1 agccaaagat aataaaagtt taggaaaatt taaactaaag aaaattaaac gggcaatgcg
       61 tggggtaccc caaattgaag ttacttttga tattgatgct aacggaatcg ttaatgtatc
      121 agctaaagat ttagcgagtg gaaatatgca aagtattact attgaaaata cttcgaatat
      181 gtctgatgat gatatcgaaa gagctattca tgaagctaaa caatatgaac agcaagatgc
      241 tgttactaaa gagcgtttag tttttaaaaa tgaagttgaa aatttgattc ttacggttga
      301 agcagcttta tctaaaaatg gtaaacaaat tgataaagca atgaaatcaa atattaaaaa
      361 cgaacttgca agctttaaaa agttaactaa aaaagttgat tttgaaacat gtgatcagat
      421 tcagtttgat gaaatcaaaa cagcaaaact taatttggaa acagttgcag catcattgtt
      481 atcaatgcaa taatccttgg ttataaccaa ggatttttta ggaggaaaat atgatagacg
      541 agattttagc ttacaatcgg gcatttgtga ctaataaagg atataagcct tatacgacga
      601 gtaaatatcc tgatcgcaaa ttagcgattg ttacttgtat ggatacccgt ttgattgaat
      661 tattaccagc ggctctagga ataaaaaacg gagatgcaaa aatcataaaa aatgctggtg
      721 gagttattgt tcaccctttc ggtagtgcgg tacgaagttt attgattgct atttatgaat
      781 tgaatgttga ggaaatcatg attatcggac atactgattg tggagtagga agtatcgata
      841 tagaggcaat gttaaagaag atggaaaagc gtggaatcag tgaaacggtt attagggatc
      901 ttggatattg tggaattgat tttaataaat ggctgggcgg ttttgatgat gttgagatat
      961 ctgttaaaga aagtgttagt ttgttaagac gacatcctct gttgcctaaa aatattgaga
     1021 ttcatggact agtgatggat tcaaggacgg gcgctttaag tttagtggaa aaaaattgat
     1081 tttttttgtt taaagagcat catgagattc tagttttgtt actagaatct tttaattatg
     1141 taaagaaata cggtataatg gaaaagtaga aatatcttaa cttaatatat cttgtcttgt
     1201 taactatttc acttgacaat gagtatgatt tgtgggatat tttatctaat gtacgagaac
     1261 tttgaaatta agtaaatggg gaatagttaa atgaatgata ttattgaaga aattcaaaag
     1321 ttaaaaaaag aaaaaaatgc tgtcattttg gcacattatt atgttgatgg tgcaatccag
     1381 gatattgcag attatttagg tgattcttac tatttatcaa aaattgctgt tgattgtcca
     1441 caagatgtaa ttgtttttgc tggagttgaa tttatgggtg aaagtgctaa attattaagc
     1501 ccaaataaga cagttttaat gcctgataag gatgctgatt gtccaatggc tcatatggtt
     1561 gatgaatatt ttattgcccg tcttcgtaaa gagtatgaag atctatgtgt tgtatgctat
     1621 attaactcaa ctgctgaaat taaaacaatg tcagatgttt gtgtaacatc atcaaatgct
     1681 aagagaatcg ttgaagctct accaaataaa aatattttat ttattccaga tcaaaatcta
     1741 ggaaaacata ttgctgaatt agtaccaagc aagaatttct tattctgtaa tggatattgc
     1801 ccaacgcatt atcggattac accagaagat attttaacag ctaaagaaaa acatccgggg
     1861 gcacctgtat tgattcatcc tgaatgtaaa ccagaatgtg tagagttggc agattatgct
     1921 ggcagcactt cgggaattat tgattatgca acaaatgatc aggaacatga tgagttcatt
     1981 gttgtcactg aaataggcgt gattcatgaa atggaaaaac gtaatcctgg aaagaaattc
     2041 tatccagcaa ctgataaatt agtatgtcca aatatgaaga aaaacacgct tgaaaaagta
     2101 cgtgattgtc taaagaataa tactaatcaa gtagagcttg atgaagagtt tatggaaaaa
     2161 gctaagaaac cattggaaag aatgcatgaa ttagcaaaat aagaaggtgt aaataatgaa
     2221 taataattat gacgtaatca ttgttggaac aggagctgca gggttatttg cagggttatg
     2281 tttacctgct gatttaaaag ttttaatgat tactaaagat aaagttgaaa atagtgattc
     2341 ttatctagct caaggaggaa tctgtacttt aaagagtgct gatgattttg atgcgtttta
     2401 tcaagatact ttaaaagccg gtcgtaacga aaacaatcct gaatcagtta aaatcatgat
     2461 tcagcaggca cctcaaatta tgaaagattt gatggattat ggtgttgaat ttgatcgcga
     2521 tactgaagga aatttagctt atacacgtga aggtgctcac tcggaatatc ggattttaca
     2581 tcatcaggat gttactggta aagaaatcac ttctaaatta attaaacaag ttgaaatgcg
     2641 gaataatatc acaatggtag aagatgctac aatgctggat attattaatc atgataatat
     2701 tgcaacagga atcgttatgg aaaataatgg tgaaattatt caaattaatg ctaaagttat
     2761 tattcttgca actggtggga ttggtggatt atttacccac tcatctaatt ttagacatat
     2821 caccggtgac agttttgcaa ttgccttaag aaataatatt g
//
LOCUS       DAOMTA010000356         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1500_length_2856_cov_2.847197, whole genome shotgun sequence.
ACCESSION   DAOMTA010000356 DAOMTA010000000
VERSION     DAOMTA010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_2856_cov_2.847197"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLK68_09625"
     CDS             complement(<1..814)
                     /locus_tag="PLK68_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM90701.1"
                     /translation="MKKVFGVLLTLVLCLGLTGCGGSGDDSSASGNDVSGNVSLNGST
                     SMEKFVNALKEAITEEYPNLTLEPQFTGSGAGIEAVTNGTADIGDSSRALKDEEKAKG
                     IEENIVAIDGIAVVTHKDNKVSDLTTDDLKKIYTGEITNWKDLGGDDQAIVVVGREAG
                     SGTRGAFEEILGIEDACKYAQELNETGAVMAKVSETAGAIGYVSLDVVDDTVKSLKLD
                     GVKASEKTIKDGSYTLQRPFVMATKGKISEQSKEVQAVFDFINSEAGQKVIEQ"
     assembly_gap    840..870
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(939..1925)
                     /locus_tag="PLK68_09630"
     CDS             complement(939..1925)
                     /locus_tag="PLK68_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HRM90702.1"
                     /translation="MGEKLYYVFKIIKEAREPISAKDILKRLENYEIFLDIKTVYSLI
                     KKLNDFYYCLSNKQLIKTIRRRGYIIDEDFFEDGQLQLLIDSVLFNPNLDKKSANDLV
                     NKLALISSVIQMERLNTEHQNDNELTYDLLLNLTTVIKAINNHKNIAFKYISYDIKDN
                     ALQEVYHTNGNLNPETYVISPYKLILRNSNYYLIGYFDKRKDSLSVYRIDRMRIVRNH
                     SSSYEDIQDRFDMEKEFENNVNMYVSNERIDLKIAFESSVLREVVNQFGQDINVNKCF
                     DGRIEAFIKDVALSDGLIGWIMMLQDKVEVVFPLSLKEIVKTRIRAMLRIYE"
     gene            complement(1939..2571)
                     /locus_tag="PLK68_09635"
     CDS             complement(1939..2571)
                     /locus_tag="PLK68_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90703.1"
                     /translation="MKKQSYQKVIDKDIIEVKQYLLDISEGYWMQDIHDLINISMDVK
                     IIRKKLMRRKDLELAVFSKIKKLIDQAQGLNEMENHLIMMNLLLDKHYSPMLTYKYKL
                     LNYIIENGGFSIETYCLLRHLIKFTNNNLNDFIMALATRLNFSNERYHYLASHILLLE
                     KQYKKVYNHLEYITIDERLGRYLPALYNFSPRLYNKYARMMYIPLNLAIM"
BASE COUNT          962 a          519 c          278 g         1066 t
ORIGIN      
        1 cttgttcaat aactttttgt ccagcttcac tattgataaa atcgaacaca gcttgtactt
       61 ctttactttg ttcactgatt tttcctttag tagccattac gaatggtctt tgtaaagtgt
      121 agcttccatc tttaatagtt ttttcagaag cttttacacc atctaatttt aatgatttaa
      181 ctgtgtcatc aacaacatct aaagaaacat aaccaattgc tcctgctgtt tcactaactt
      241 ttgccattac tgcaccagtt tcgtttaatt cttgagcata tttacaagca tcttcaattc
      301 ctaaaatttc ttcaaatgct ccacgagttc ctgaaccagc ttcacgtcca acaacaacga
      361 ttgcttgatc atcaccacct aaatctttcc agtttgtaat ttcaccagta tagattttct
      421 ttaagtcatc agtagttaaa tcagacactt tgttatcttt atgagtaact acagcaatac
      481 catcaatagc aacaatattt tcttcaattc cttttgcttt ttcttcatct tttaaagctc
      541 ttgaagaatc tccgatatca gcagttccat ttgtaacagc ttcaatccct gcacctgaac
      601 cagtaaattg tggttctaat gttaagtttg gatattcttc agtaatcgct tcttttaaag
      661 catttacgaa tttttccata gatgtagacc cgttaaggct aacatttccg cttacatcgt
      721 ttccacttgc agaagaatca tcaccagatc ccccacatcc agtaagccct aagcataata
      781 ctaatgttaa taaaactccg aatacttttt tcatatttaa taatcccttt ctttatcttn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgatatatca ataaattgta tttattatgt
      901 taagtttacg taaagaaaaa agagaaccct caagttctct attcatatat ccttaacata
      961 gcccttattc gtgtctttac aatctctttt agacttaaag gaaaaacaac ttcaacctta
     1021 tcttgaagca tcattatcca gccaattaaa ccatctgata aagccacatc tttaataaat
     1081 gcttcaattc gaccatcaaa gcatttattt acattaatat cttgtccaaa ctgattaaca
     1141 acttctctta gtacactaga ttcaaaagca atctttaaat cgattcgttc attactaaca
     1201 tacatattaa cattattttc aaattctttt tccatatcga aacgatcttg aatatcttca
     1261 taactactgc tatgatttct cacgatacgc attcgatcaa ttcgataaac acttaaacta
     1321 tcttttcttt tatcaaaata accaatcaaa taataattag aatttctaag aatcaattta
     1381 tatggggaaa taacatatgt ttcaggatta agattaccat tagtatggta tacctcttga
     1441 agagcattat ctttgatatc ataactaata tatttaaaag caatattttt gtggttatta
     1501 attgctttga ttaccgtagt taaatttaat aatagatcat atgttaattc attatcattt
     1561 tgatgttccg tatttaaacg ttccatttga ataaccgaac taatcaaagc taatttatta
     1621 actaaatcat tcgctgattt tttatcaaga ttgggattaa ataaaacact gtcaattaat
     1681 agctgcagct gtccatcttc aaaaaaatcc tcatcaataa tataacccct tcttctaatt
     1741 gtttttatta actgcttatt actcaaacaa taataaaaat cattaagttt ttttattaaa
     1801 ctatataccg ttttaatatc tagaaaaatc tcataatttt ccaaacgctt taaaatatct
     1861 ttagcactaa tcggctccct agcttcctta atgattttaa aaacataata cagtttttca
     1921 cccataattt ccttactcct acattatcgc taaatttaat ggtatataca tcattctagc
     1981 atatttgttg tataatcggg gactaaagtt atacaacgcc ggtaaataac gtcccagtct
     2041 ttcatcaatt gtaatgtatt caagatgatt atatactttt ttatattgct tttctaataa
     2101 taagatatga cttgcaagat agtgataacg ttcattacta aagtttaaac gtgtagccaa
     2161 agccatgata aaatcattaa gattattatt agtaaactta attagatgtc ttaataaaca
     2221 atacgtttca attgaaaatc caccattttc aataatataa tttaataact tatatttata
     2281 tgtcaacatc ggactataat gtttatccaa taaaagattc atcatgatca aatgattttc
     2341 catctcattt aaaccctgag cctgatcaat tagttttttt atcttactaa atactgctaa
     2401 ttctaaatcc ttacgtctca ttaatttttt gcgaataatt ttaacatcca tacttatatt
     2461 aataagatca tggatatcct gcatccaata tccttcactt atatccaata aatactgttt
     2521 aacttcaata atatccttat ctataacttt ttgataactt tgctttttca tgatatcact
     2581 cctttaacta cttctattat agtaatttaa aaaattttta taccaaaaat aagctaatta
     2641 aaaaccgtta ttttggtttt taattatttt gttaaaattt gtcgtaagaa aaactaagga
     2701 aatccatttc agataaaatg ttcttcctta gctatcaata aaatatattt atttaaatta
     2761 gcaaaaagtc ttcaatccct taacaattat gcgagtcttt tcttaataac aaaatgaata
     2821 attctttcaa taaatagtcc aaaaatagat aaaatt
//
LOCUS       DAOMTA010000357         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1504_length_2852_cov_1.851627, whole genome shotgun sequence.
ACCESSION   DAOMTA010000357 DAOMTA010000000
VERSION     DAOMTA010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1504_length_2852_cov_1.851627"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLK68_09640"
     CDS             <1..370
                     /locus_tag="PLK68_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRM90704.1"
                     /translation="KNQTDFIVVNFANGDMVGHTGIIPAAIKAVSVVDECVGEVYNKV
                     LELGGTMLITADHGNSEMLLDEDNNPFTAHTTNEVPLIVTNSHLELKEGGKLGDLAPT
                     ILQLLGLEIPAEMDGESLLK"
     gene            370..885
                     /locus_tag="PLK68_09645"
     CDS             370..885
                     /locus_tag="PLK68_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM90705.1"
                     /translation="MKKLLSLIVCVLMISGCQDASINRIKKDNSLSGLHEITYDELEN
                     KLSSDDTFVLYIGRPDCGDCKEFHPILTSYLEENKGTYLYYLNIQAFRDAAMQEGASE
                     KEIDFYKNIREELDFNWTPILKLVNKGETISEYTYLSEEYYEIKDDTKKAQAKEKFVE
                     DFKTWMANIYE"
     gene            894..>1272
                     /locus_tag="PLK68_09650"
     CDS             894..>1272
                     /locus_tag="PLK68_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM90706.1"
                     /translation="MKQCPHCKKNIPDSAKVCPHCGTRLEKGYQPMKRTNTFPNYLYG
                     ILALVLIFSPLISSMLFGNFLGDGTASNTVTTPDKAITLGPLGEVNINKEVTEYQFGS
                     LKDFDKLVTNSDSYVKKIKQLEND"
     assembly_gap    1273..1282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1283..>1586
                     /locus_tag="PLK68_09655"
     CDS             <1283..>1586
                     /locus_tag="PLK68_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM90707.1"
                     /translation="AITDKYGKTTIDKDYDFYVTDQNNVYTSLNYDLKIGKNETMSIS
                     FSYDLSGTTNAVNIGYTINGFKDFEAMKINEQSYPMLKEIVKLINGDDMYVSFNKAG"
     assembly_gap    1587..1596
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2019..2723
                     /locus_tag="PLK68_09660"
     CDS             2019..2723
                     /locus_tag="PLK68_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM90708.1"
                     /translation="MKKKYLKIDNIPAVIWGAGSSKVFLAVHGNKSNKEDIVIEMLAK
                     TAVNRGYQIVSFDLPKHGDRVYEDTLCNAQNCTEELLKIYDYVKGKYEKISLWACSLG
                     AFFSLLAYQCVKFNQCLFLSPVVDMQRLIENMMIWFEINEEKLKEQKMIETPIGETLY
                     YDYYCYVKEHSVTEWNSQTMILYGENDNLCEYEYIKKFASHFNCDLKIMENGEHFFHL
                     KNQLEFYQNWLEEKVK"
     gene            2733..>2852
                     /locus_tag="PLK68_09665"
     CDS             2733..>2852
                     /locus_tag="PLK68_09665"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM90709.1"
                     /translation="MKKIAVLTSGGDAPGMNGAIRAIVRVGIKHGFEVYGVYEG"
BASE COUNT         1077 a          306 c          550 g          899 t
ORIGIN      
        1 gaaaaatcaa actgatttta tcgttgtaaa ttttgcaaac ggcgatatgg ttggacatac
       61 aggaattata ccagcagcaa ttaaagctgt gagtgttgtt gatgaatgtg taggtgaagt
      121 atacaataaa gttcttgaat taggtggaac aatgttaatt actgctgacc acggtaatag
      181 tgaaatgttg ttagatgaag ataataaccc atttacagcg catactacaa atgaagtgcc
      241 tttaattgta acaaattctc atttagaatt aaaagagggt gggaaattag gagatttagc
      301 gcctacgatt ttacaattat taggacttga aattccagct gaaatggatg gagaatcttt
      361 acttaaataa tgaaaaaatt attaagttta attgtatgtg tattaatgat tagtggatgc
      421 caagacgcat ccattaatcg tataaaaaaa gacaatagtt tatcaggtct tcatgaaatt
      481 acttatgatg aattagaaaa caaattatct agtgatgata cttttgtttt atatattggt
      541 cgtcctgatt gcggagattg taaagagttt catccgattt taacatcgta tttagaggaa
      601 aataagggta cttatttata ttatttaaat attcaggcat ttcgtgatgc cgccatgcaa
      661 gagggggcat ctgaaaaaga aattgatttt tataaaaata ttcgtgagga gttagatttt
      721 aattggacac caattttaaa attagttaat aaaggtgaaa caattagcga gtatacttat
      781 ctaagtgaag agtattatga aataaaagat gatacaaaaa aagcccaggc aaaagaaaaa
      841 tttgtggaag actttaagac ctggatggca aatatatatg agtaggtgat tgcatgaaac
      901 aatgtccaca ttgtaaaaaa aatattcctg atagtgccaa agtttgtcct cattgtggta
      961 cacgcttaga aaaaggctat caacccatga aaagaacaaa tacatttcct aattatttat
     1021 acggtatttt agctttagta ttgattttct caccgctgat ttcatcaatg ttatttggta
     1081 attttttagg tgacggaact gcaagtaata ctgttactac accggataaa gcaattacac
     1141 taggtccact aggtgaggtt aatattaata aagaggtaac agaatatcag tttgggagtt
     1201 taaaagattt tgataaacta gtaactaata gtgatagtta tgttaagaag atcaagcagt
     1261 tagaaaatga ttnnnnnnnn nngcaattac tgataagtat ggtaaaacaa cgattgataa
     1321 agattatgat ttttatgtaa ctgatcaaaa taatgtttat acaagtctga attatgattt
     1381 aaaaattggt aaaaatgaaa caatgtcaat tagtttttca tatgacttat caggaacaac
     1441 taatgctgtt aatataggtt atactattaa cggctttaaa gattttgaag ctatgaaaat
     1501 caacgagcaa agttatccga tgttaaaaga aattgttaaa ttgattaacg gtgatgatat
     1561 gtatgtaagt tttaataaag ctggagnnnn nnnnnnattg tttaaaaaag tttaatcagt
     1621 tagaaatcag tttaatcaat tagaaaatga ttttaatgaa cgaaatgaaa gtattggtaa
     1681 ttatggaata ggaatcactc aatcagaaga tgatacaaaa gtgtcaatga gaattctttc
     1741 aagcgaagat ggttatcgat taaaaatcac atataaaact aaagctgata tgaataaatt
     1801 agtgggtaat agtaaaggaa ctgaataatc agttcctttt ttgctgtgct gcgcacgaca
     1861 taaaactagg tgatgaaagc ccgctgtgga ggtttcgtgc taccaaccac cagccaaaga
     1921 caaggctgca acataaactg ttgtagtatt attttttgca aaagctaaat attaaatttg
     1981 gactatgtta taatcattga taaggaagtg gtgggaagat gaaaaagaaa tatttaaaaa
     2041 ttgataatat ccctgcagtt atctggggag cagggagttc aaaagttttt cttgcagttc
     2101 atgggaataa atctaataaa gaagatattg ttatagaaat gctagcaaag acagcagtaa
     2161 atagaggata tcaaatagtt agttttgatt tgccaaaaca tggggatcga gtatatgaag
     2221 atactttgtg taatgcacaa aattgtacag aggaattatt aaagatatat gactatgtta
     2281 aaggaaaata tgaaaagata agtttatggg cctgtagctt aggagcattt tttagtttat
     2341 tagcttatca atgtgttaaa tttaatcagt gtttattttt atcaccagtt gttgatatgc
     2401 aacgtttaat tgaaaatatg atgatttggt ttgaaataaa tgaagaaaaa ttaaaagaac
     2461 aaaaaatgat tgaaacacct attggggaaa cattgtatta tgactattat tgctatgtta
     2521 aagaacattc agtcaccgaa tggaatagtc aaacaatgat tttgtatggt gaaaatgaca
     2581 atttatgtga atatgaatat ataaagaaat ttgccagtca ttttaattgt gatttaaaaa
     2641 tcatggaaaa tggggaacac ttttttcatc taaagaatca actagagttt tatcaaaatt
     2701 ggttagaaga gaaagttaaa tgaggtgaaa aaatgaaaaa aatcgcagta ttaacttctg
     2761 gcggtgatgc tcctggaatg aatggagcaa tcagagcaat tgtcagagta ggaataaaac
     2821 atggatttga agtttatgga gtatatgaag gc
//
LOCUS       DAOMTA010000358         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1509_length_2841_cov_2.083274, whole genome shotgun sequence.
ACCESSION   DAOMTA010000358 DAOMTA010000000
VERSION     DAOMTA010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_2841_cov_2.083274"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1004)
                     /locus_tag="PLK68_09670"
     CDS             complement(219..1004)
                     /locus_tag="PLK68_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90710.1"
                     /translation="MLELKDVSVVFNEGTVNEKVALNNINLALDKGDFVTIIGSNGAG
                     KSTLFQAISGAVDTKRGNIILNGRDITFEPEYKRSKGIGRLFQDPLKGTAPNMTIEEN
                     LHLSNQRGKHFSLSLMSHRHREEFKKALMELELGLEERLDSKVGLLSGGQRQALTLLM
                     ATLVTPDLLLLDEHTAALDPKTALKVLELSQKIVEEHQITTLMITHNMEDALKYGNKT
                     MIMKDGQIIAMIEGKEREEMTVEGLIHLYSTSSQEYTDRVLLK"
     gene            complement(1004..>1226)
                     /locus_tag="PLK68_09675"
     CDS             complement(1004..>1226)
                     /locus_tag="PLK68_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90711.1"
                     /translation="ASIIVGTTFIKKEKIIFQLVAVVFGAIFYRAVLTVALQLGLPSG
                     YLKLLSAVLVIVAIASTNGAFRSKKRGRN"
     assembly_gap    1227..1236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1237..1853)
                     /locus_tag="PLK68_09680"
                     /pseudo
     CDS             complement(1237..1853)
                     /locus_tag="PLK68_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535879.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1858..>2841)
                     /locus_tag="PLK68_09685"
     CDS             complement(1858..>2841)
                     /locus_tag="PLK68_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM90712.1"
                     /translation="NKLFKLMAVSALTMTALTGCGNSSSNGDVKEVAIIQYVEHTSLN
                     TIKDAFDEQMEALGYKEGENIKYTFKNAQGDMNTAPSIIQDFKSSKKDCVVAIATPVA
                     QTAAEMSTDTPVVFAAVTDPIKAGLTTSLEKPDKNITGTSDEIQVEMILERALQVNPD
                     LKKIGVIYNKGEVNSVTNIAKAKAFCDDKGIEMIETTVTGVNEVQSAIDVLTSKCDAV
                     FAPNDNTVANAMDMVGPACAKAKVPLYVGADSMVQDGGFLSVGINYEDLGKETANMVD
                     KILKGEKVENIPVKVFKENLSVYVNTKVLKQLGITLPNEIKNDKAYVEIKE"
BASE COUNT          936 a          560 c          336 g          999 t
ORIGIN      
        1 tataaataag tactttttta gctttgtcgt agagtacatt actctacgac tttcttttat
       61 tatttacaaa aaaaatattt ttattcaaaa aatgtagaat ttgataaact atattatcta
      121 tttaagtatc taattctaat actcctgata ctgtatccca taattatttc aataaaaaaa
      181 catgaactca aggtaaatcc taagttcatg ttttttgttt attttaataa aactcggtcc
      241 gtatattctt gagaagaagt agaatacaaa tgaatcaagc cctctacggt catttcttct
      301 ctttctttgc cttcaatcat tgcaatgatt tgtccatctt tcataatcat tgttttatta
      361 ccgtatttta gagcatcttc catattatga gtaatcatca gtgtagtaat ctgatgctct
      421 tcaacaattt tttgtgataa ttctaaaacc tttaatgccg tttttggatc taaagcagct
      481 gtatgctcgt ccaataacaa taaatctgga gttactaaag ttgccattaa taacgtcaat
      541 gcttgtcgct gtcctccaga caacaatcca actttagaat ctaatcgttc ttccaaacct
      601 aactctaatt ccatcagtgc ctttttaaac tcttcacgat gacgatgaga cattagactt
      661 aaactaaaat gtttacctct ttgatttgac aaatgaagat tttcttcaat tgtcatattt
      721 ggggctgtac cctttaaagg atcttgaaac aatcttccaa tcccttttga gcgcttgtac
      781 tcaggctcaa aagtaatatc acgaccattt aaaataatat ttcctcgttt tgtatcaact
      841 gctccagaaa tagcttgaaa taaagttgat ttaccagcac cattacttcc aatgattgta
      901 acaaaatccc ctttatcaag tgcaagatta atattattaa gggcgacttt ttcattaact
      961 gtaccttcat taaaaacaac tgatacatcc tttaattcta acattaattg cgcccccttt
     1021 tcttacttct aaatgctcca ttagttgaag caattgccac aataacaaga actgctgata
     1081 acagctttaa atatcctgaa ggaagtccca gctgtagagc aactgttagg actgcacgat
     1141 agaaaattgc tccaaataca actgcaacta actgaaaaat aattttttct ttctttataa
     1201 atgttgttcc gacaataata ctagctnnnn nnnnnnatat ctgcaaagct ttgatgttgg
     1261 gcaaatacag caccactcat agcgactaag ccatttgcta atgctaaacc taaaatcttc
     1321 attttatcag tatcaataga gcttgctcga accatatctt cattatcacc acaggctcta
     1381 agtgataatc ctagctgagt tcttaagaaa aaatctaaaa tcacacaaat aacaattaca
     1441 atcaaatata ttaaaataat tttcccataa ggatcaaatg cagtaaaact gctaaaaata
     1501 gtattattac caaacagtga taaattagga gtatcattca taattgctaa attaattgaa
     1561 tataatccag tcattgttaa aatcccagat aaaagcggca taattttgaa ttttgtaatt
     1621 aatagaccag taactacccc agctgctgca ccaatcacaa atgaacctat taatcctaca
     1681 tatggcattg aattaatcgt aaacatcccg ctaacagcac atcccaaagc gaaacttcca
     1741 tcaaccgtta aatctggcaa atttaacact ttataactga tatataaacc aagtgagaga
     1801 atggcaaaaa ttcctcctaa ctccagtgct cctaaaataa ccgtctgatt cataaaacta
     1861 ctcctttatt tctacatatg ctttatcatt tttaatttca ttaggcaaag taatacctaa
     1921 ttgttttaat acttttgtat ttacataaac gcttaaattt tctttaaata ctttaactgg
     1981 aatattttcc actttttcac cttttaagat tttatcaacc atatttgcag tttcttttcc
     2041 aagatcttcg taattaattc caacacttaa aaaaccacca tcttgaacca ttgaatcagc
     2101 gcctacatat aaaggtactt ttgctttagc acaagcaggt cctaccatat ccattgcatt
     2161 tgctacagta ttatcatttg gagcaaagac tgcatcacat ttactagtta atacatcaat
     2221 tgcactttgc acttcattta ctccagtaac agttgtttca atcatttcaa ttcctttatc
     2281 atcacaaaaa gcttttgctt tagcaatatt agtaactgaa tttacttcac ctttattata
     2341 aatgactcct atttttttca aatccggatt aacttgtaaa gctctttcta aaatcatttc
     2401 aacttgaatc tcatcactcg ttcccgtaat atttttatct ggtttttcaa gagatgtagt
     2461 taaaccagct ttaattggat cagttaccgc tgcaaaaaca actggtgtat cagttgacat
     2521 ttcagctgct gtttgagcta caggagttgc gattgcaact acacaatctt ttttactaga
     2581 tttaaaatct tgaataatcg acggagctgt gttcatatct ccttgagcat ttttaaaagt
     2641 atacttaata ttttcacctt ctttatatcc caaagcctcc atctgttcat caaaagcatc
     2701 tttaattgta tttaatgatg tatgttcaac atattgaata atcgctacct cttttacatc
     2761 accattacta ctgctatttc cacatcctgt taaagctgtc attgttaaag ctgatactgc
     2821 cattaattta aatagtttat t
//
LOCUS       DAOMTA010000359         2841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1510_length_2841_cov_2.020818, whole genome shotgun sequence.
ACCESSION   DAOMTA010000359 DAOMTA010000000
VERSION     DAOMTA010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1510_length_2841_cov_2.020818"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /gene="ffh"
                     /locus_tag="PLK68_09690"
     CDS             complement(<1..1333)
                     /gene="ffh"
                     /locus_tag="PLK68_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRM90713.1"
                     /translation="MAFDSLSERLQGTLKKVSGQGRLTEKNMEEMLSEIRLALLEADV
                     NFQVVKEFIAATKEKALGQDVLGSLKPGQVVVKIVHDELVSLLGTSVAEVDFDKKPTV
                     IMMVGLQGSGKTTTSGKIAKLITKKYGKKPLLVAADIYRPAAVDQLKTLGEQLNVPVY
                     EKGTSQSAESIVEEAMTFARDHNNDVIIIDTAGRLHIDEPLMNELANIKKIAHPSEIL
                     LVVDALTGQDIVNVASSFNEQLSITGAVLTKLDGDSRGGGALSIRHITNVPIKFIGTG
                     EKLDAIDLFYPDRMADRILGMGDVVSLVEKVQDVYDEKDTMKAYKKMQSGQFGLDDML
                     SQMQQLRKLGPLSGIMKMIPGMPKLPKMNDEDSEKKLKMTESIIFSMTKEERRDPSII
                     TLSRKERIAKGCGKDVAAVNRLLKQFEESKKMMKMLGNVDPNTGMPMPGGRA"
     gene            complement(1346..1675)
                     /locus_tag="PLK68_09695"
     CDS             complement(1346..1675)
                     /locus_tag="PLK68_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRM90714.1"
                     /translation="MESSLEKKQRVNLLMDCYSDLLTEKQQTYLEYYYQEDYSLSEIA
                     QILDVSRNAVFDNLKKAVHSLENYEEKLQLLKKHQERLDLIQRIEDDISSDHKSLEEY
                     LELLRKI"
     gene            complement(1677..2660)
                     /gene="ftsY"
                     /locus_tag="PLK68_09700"
     CDS             complement(1677..2660)
                     /gene="ftsY"
                     /locus_tag="PLK68_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRM90715.1"
                     /translation="MGFFSQIKEKFVGKSAKQNEKYVAGLDRSNSTFSDRINELAARF
                     REINDEYFEELENILIMSDVGVSMVMKIVEEIKNEVRLQNITDPKEINEIIVDKMFVI
                     YANDSVMTTKINYAEEGLTVILMVGVNGAGKTTTIGKLANRIVNDEGKKVMVAAGDTF
                     RAGAIDQLAVWAQRVGVDIVKGKEGGDPSAVVFDALKQAKEKNVDVLICDTAGRLQNK
                     VNLMNELEKMNRIIKREVPDAPHETLLVIDATTGQNGVSQAVEFSKITDVSGLVLTKM
                     DGTAKGGIVLSIKDQLNIPVKFIGLGESVDDLQEFDLDQYIYGLCKNLVEE"
BASE COUNT          846 a          624 c          358 g         1013 t
ORIGIN      
        1 ttgcacgtcc ccctggcatt ggcatacctg tattaggatc aacattacct aacatcttca
       61 tcattttttt agattcttca aattgtttta gcaagcggtt aactgctgca acatctttac
      121 cacaaccttt agcaattctc tcttttctag ataaagtaat aatacttgga tctcgacgtt
      181 cctctttggt cattgaaaaa ataattgatt cagtcatttt taatttcttt tcagagtcct
      241 catcattcat ttttggtaac ttaggcattc ctggaatcat tttcataatt cctgatagtg
      301 gccccaattt ccttaactgc tgcatttgag aaagcatgtc atcaagacca aattgtcccg
      361 attgcatctt tttataggct ttcattgtat ctttttcatc atatacatct tgtacttttt
      421 caactagaga tacaacatcg cccattccca aaatacgatc agccatacga tcaggataga
      481 ataaatcgat agcatctaat ttttcaccag taccaatgaa tttaattggt acatttgtaa
      541 tatgacgaat tgatagagct cccccaccac gagagtcacc atctaattta gttaaaacag
      601 ccccagtaat gctcaactgt tcattaaatg aactggcaac attaacaata tcttgtccag
      661 ttaaagcatc aacaactagt aaaatttcac ttggatgagc gatcttttta atattagcca
      721 gctcgttcat taatggttcg tcaatatgta aacgccccgc agtatcgata ataattacgt
      781 cattattatg gtcacgagca aatgtcatcg cttcttctac gattgattca gcagattgtg
      841 atgtcccttt ttcgtagaca ggaacattca attgttcacc taatgttttt aactgatcca
      901 cagcagctgg gcgataaata tctgcagcaa ccaacaatgg ttttttacca tatttcttgg
      961 taattaattt tgcgattttt ccagaagttg tagttttccc agaaccctga agtccaacca
     1021 tcataataac agttggcttc ttatcaaaat caacttccgc aacacttgtt cccagtaacg
     1081 aaactagttc gtcatggacg atttttacaa caacctgtcc cggttttaat gaacctaaaa
     1141 catcttgtcc caacgctttt tcttttgttg cagcaataaa ctcttttaca acttgaaagt
     1201 ttacatctgc ctctaataat gctaagcgaa tttcgctaag catttcttcc atattttttt
     1261 cagtcaaacg cccttgtcca gaaactttct ttaaagtccc ttgcagacgt tctgataatg
     1321 aatcaaatgc cataaatacc cctttctaaa tcttccttaa taattctaaa tattcttcta
     1381 gacttttatg atcactagat atatcatctt caattctttg gatcaagtca agcctttctt
     1441 gatgtttttt taacaactgc agtttttctt cataattttc taaactatga actgcttttt
     1501 ttaaattatc aaatacagca ttacgactaa catccaaaat ttgtgcaatt tccgataaag
     1561 agtaatcttc ttgatagtaa tattctaaat atgtttgctg tttttctgtc aacaaatcac
     1621 tataacaatc cattagtaga ttaactcgtt gttttttctc taaacttgat tccatactat
     1681 tcctcaacta ggtttttgca taacccatat atatactggt ctaaatcaaa ttcttgtaaa
     1741 tcatcaacac tttctcctag accaatgaat tttacaggta tattcaattg atctttgata
     1801 gaaagaacaa tcccaccctt agcagttcca tccattttgg ttagaactaa ccctgagaca
     1861 tcagtgatct tagaaaattc cacagcttgt gaaacaccat tttgaccagt tgttgcatca
     1921 ataactaaca gtgtttcatg cggtgcatca ggtacttcgc gtttaataat acgattcatt
     1981 ttttctaatt cattcattaa gttgacctta ttttgaagac gaccagcagt atcacaaatt
     2041 aaaacatcaa catttttttc ttttgcctgt tttaaagcat caaataccac tgctgaagga
     2101 tcaccgccct ctttaccttt aacaatatcg acaccaacac gctgtgccca aactgctaac
     2161 tgatcaatcg ctccggctct aaaagtatct ccagctgcaa ccattacttt cttaccttca
     2221 tcattaacga tacgattagc taatttccca attgttgtag tcttaccagc cccgtttact
     2281 ccaaccatta aaattactgt taatccttcc tcagcatagt taatcttagt tgtcatgaca
     2341 ctatcatttg cataaataac gaacatttta tcaacgatga tctcattaat ctcttttggg
     2401 tcagtaatat tttgcaaacg tacctcgttt ttaatttctt caactatctt cattaccatc
     2461 gaaactccaa catcggacat aatcaaaata ttttccaatt cttcaaaata ctcatcattg
     2521 atttctctaa agcgagctgc aagttcattg attcgatctg aaaaagtact attagaccga
     2581 tctaatccag ctacatattt ttcgttttgt ttagcagatt taccaacgaa tttttctttt
     2641 atttgactaa aaaatcccat acgattcctc ctaactttga tctgttaaat ctattgcttc
     2701 ttctaatttg acactaacta actttgtaac tcccttttgt tgcattgttg ctccataaag
     2761 aaggtcacat tcttccattg ttccttcacg atgggtaacc acaataaatt gcgtcgttcc
     2821 cgaaaattct cttaaatatt t
//
LOCUS       DAOMTA010000360         2836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1512_length_2836_cov_3.067242, whole genome shotgun sequence.
ACCESSION   DAOMTA010000360 DAOMTA010000000
VERSION     DAOMTA010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_2836_cov_3.067242"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..410)
                     /locus_tag="PLK68_09705"
     CDS             complement(102..410)
                     /locus_tag="PLK68_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HRM90716.1"
                     /translation="MDEQEQIVINLIVNSGSARSSAIEAIQYAKAGDLAKADESLQQA
                     KETVNEAHHSQTELIQAEIRGEKAPLNLLMVHAQDHLMTALVVIDLAQEFIDVYKKIG
                     "
     gene            complement(560..1744)
                     /gene="tuf"
                     /locus_tag="PLK68_09710"
     CDS             complement(560..1744)
                     /gene="tuf"
                     /locus_tag="PLK68_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRM90717.1"
                     /translation="MAKEKFDRSKAHVNIGTIGHVDHGKTTLTAAITTVLSKDGQAQA
                     MDYAAIDAAPEEKERGITINTAHVEYQTATRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLSRQVGVPYIIVFLNKCDMVDDEELLDLVEMEVRELLNE
                     YDFPGDDTPVIRGSALKALEGDPKWVPAIHELMEAVDSYIPTPTRDTDKPFLMPVEDV
                     FTITGRGTVATGRVERGQLNLNDPLEIVGIHETKNTVATGIEMFRKLLDYAESGDNVG
                     VLLRGVNREEIQRGQVLAKPGSVNPHKKFKSQVYILSKDEGGRHTPFFANYRPQFYFR
                     TTDVTGVIELPEGVEMVMPGDNVELTVELIAPIAIEKGTKFSIREGGRTVGSGNISDI
                     IE"
     gene            complement(1829..>2836)
                     /gene="fusA"
                     /locus_tag="PLK68_09715"
     CDS             complement(1829..>2836)
                     /gene="fusA"
                     /locus_tag="PLK68_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607407.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRM90718.1"
                     /translation="HANKRNEIQTVYAGDIAAAVGFKNTTTGDTICDEKNFVILEKME
                     FPEPVIELAIEPKTKQDQDKLGVGLSKLAEEDPTFRTFTNPETGDTVIAGMGELHLDV
                     IVDRLRREYKVEANVGAPQVAYRETIKTAAECEGKYVKQSGGRGQYGHVWIKFEPNEG
                     KGFEFVDAIVGGSVPREYINSVKVGLEDALATGMIAGYPVLDVKATLFDGSYHDVDSS
                     EMAYKVAASMALKAAGKKCDPVILEPIMAVEVTAPAEYLGSVMGDVSSRRGMIEGQEE
                     RGNAVTVQASIPLSEMFGYATDLRSFTQGRGNYTMIFDRYEPVPKSIREEIIKKNGGN
                     N"
BASE COUNT          817 a          561 c          456 g         1002 t
ORIGIN      
        1 taccatatcc cttttgtctt gtaaagcctt ttttgtcttt ttttgtaaaa aaataccact
       61 ccttcaaaaa ggaatggtat ttattcacct aactagtaaa attacccgat ttttttgtaa
      121 acatcaataa attcttgagc taagtcaata actaccaaag cagtcattaa gtggtcttga
      181 gcatgtacca ttaataagtt taatggtgct ttttctccac gaatttcagc ttgaattaat
      241 tctgtttgag aatgatgagc ttcgtttact gtttctttag cttgttgtaa tgattcatca
      301 gcttttgcta agtcaccagc ttttgcatat tgaattgctt caattgcaga gctacgagca
      361 ctaccactgt ttacaattag gttaattacg atttgttctt gttcatccat tttgatatct
      421 ccttataaca atgaattttc agatttatat ctgctcttat attataccta agtgaagggt
      481 gcttttcaag cggtttcaat tattttcaaa atgtaaaaaa agaaagccac taatgtgact
      541 ttccaaattt ctgtaattat tattcaataa tatcagagat atttccagaa cctacagttc
      601 taccaccttc acggattgag aatttagttc ctttttcaat tgcaattgga gcaattaatt
      661 ctacagttaa ttcaacgtta tccccaggca taaccatttc tacaccttct ggtaattcaa
      721 taacaccagt tacgtcagta gttctgaagt agaattgtgg tctatagtta gcgaagaaag
      781 gagtatgacg tccaccttca tctttactta agatatacac ttgagatttg aattttttgt
      841 gtggattaac tgatccaggt ttagctaata cttgtccacg ttgaatttct tcacggttaa
      901 cacctcttaa taaaacccct acgttatctc cagattcagc gtagtctaat aacttacgga
      961 acatttcgat acctgtagca acagtgtttt tagtttcatg aataccaacg atttcaagtg
     1021 ggtcatttaa gtttaattgt ccacgttcaa cacgtccagt agcaactgta ccacgtccag
     1081 tgattgtgaa tacgtcttca actggcatta agaatggttt atcagtatct ctagttggag
     1141 ttggaatata agaatcaaca gcttccatta attcatgaat agctggaacc cattttggat
     1201 ctccttctaa agcttttaaa gcagaaccac ggataactgg agtgtcatca cctgggaagt
     1261 catattcatt taataattca cgaacttcca tttctactaa gtctaataat tcttcgtcat
     1321 caaccatatc acatttattt aagaatacaa tgatgtaagg tacacctact tgacgagata
     1381 ataagatatg ttctcttgtt tgaggcattg gtccatctgt agcagcaact actaagatag
     1441 caccgtccat ttgtgcagca ccagtgatca tgtttttgat gtagtcagca tggcctggac
     1501 agtcaacgtg tgcatagtga cgagttgcag tttggtattc aacgtgtgca gtgttgattg
     1561 tgataccacg ttctttttct tctggagcag catcgatagc agcataatcc attgcttgag
     1621 cttgtccatc ttttgataat actgttgtaa tagcagcagt taaagttgtt ttaccatggt
     1681 caacgtgacc aatagttccg atatttacat gcgcttttga gcggtcaaat ttttccttag
     1741 ccatttaagg ttcctcctag tttatatttt tgtacatata cattctaatt tatttagttg
     1801 aaaaaatcaa taagattttg attggatact agttgtttcc accgtttttc ttaataattt
     1861 cttctctaat agatttaggt actggttcat aacgatcgaa gatcattgtg tagttaccac
     1921 gaccttgtgt aaatgatctt aaatcagtag catatccgaa catttctgat aaaggaatac
     1981 tagcttgcac tgtaacagca tttcctcttt cttcttgtcc ttcgatcata ccacgacgtg
     2041 aagatacgtc acccattaca ctacctaagt attcagcagg tgcagttact tctacagcca
     2101 tgattggctc taagataact ggatcacatt ttttaccagc agcttttaat gccatacttg
     2161 cagcaacttt atatgccatt tctgatgaat cgacatcatg gtaagaacca tcaaataatg
     2221 tcgctttaac atctaatact ggatatccag caatcatccc agttgcaaga gcatcttcaa
     2281 gacctacttt aacagagtta atgtattctc ttggaacaga tcccccaacg atagcatcaa
     2341 cgaattcaaa tcctttacct tcattaggtt caaatttgat ccatacgtga ccgtattgtc
     2401 cacgaccacc agattgttta acatatttac cttcacattc agcagctgtt ttgattgttt
     2461 cacgataagc tacttgtgga gcacctacgt tagcttctac tttgtattct cttcttaaac
     2521 ggtcaacgat tacatctaag tgtaattcac ccatacctgc gataactgta tcaccagttt
     2581 cagggtttgt aaatgttctg aaagttggat cttcttcagc taatttagat aatccaacac
     2641 ctaatttatc ttgatcttgt tttgtttttg gttcaatagc aagttcaata actggttcag
     2701 ggaattccat tttttcaaga ataacgaaat tcttttcgtc acagatagta tccccagtag
     2761 ttgtattttt aaaccctaca gcagctgcga tatccccagc ataaactgtt tggatttcat
     2821 tacgtttatt ggcatg
//
LOCUS       DAOMTA010000361         2830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1516_length_2830_cov_2.015135, whole genome shotgun sequence.
ACCESSION   DAOMTA010000361 DAOMTA010000000
VERSION     DAOMTA010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_2830_cov_2.015135"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(426..752)
                     /locus_tag="PLK68_09720"
     CDS             complement(426..752)
                     /locus_tag="PLK68_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6434 domain-containing protein"
                     /protein_id="HRM90719.1"
                     /translation="MKEEPVAFCRVNGLPMSGGKVEITERIGYFLDTGNISDLISSNK
                     SKCQIGIITEDSKIEENFVCSQKHRVFFKEKIDKTFSFNVPFQKWLKTNTGKIYLMQL
                     KHIMRF"
     gene            complement(873..1418)
                     /gene="frr"
                     /locus_tag="PLK68_09725"
     CDS             complement(873..1418)
                     /gene="frr"
                     /locus_tag="PLK68_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRM90720.1"
                     /translation="MINMILDDTNERMTKTIESFQRDLSSVRTGRANPNMLDRVMVNY
                     YGSPTPVNQIAGISVVEGRQLVIKPYDKSSIKDIEHGIYEADLGLTPQNDGEIIRIMV
                     PALTEERRKEFAKNVWKFAENAKVSIRNIRRDSNDEIKKTDGSEDEIKAGQERVQKLT
                     DKFVKEIDEIAKVKEKDIMTV"
     gene            complement(1420..>1893)
                     /locus_tag="PLK68_09730"
     CDS             complement(1420..>1893)
                     /locus_tag="PLK68_09730"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HRM90721.1"
                     /translation="GLAVQNALEAIGVPTRVLSAIEMRQVAEPYIRRRAIRHLEKGRV
                     VIFGAGTGSPFFTTDTTAALRAAEINADVILMAKNGVDGVYSADPKVDPNAIRFDTIS
                     YFDVLQKDLKIMDQTAITLCKDNNIDLCVFNMSVDGNIAKACNGDDIGTTISGGK"
     assembly_gap    1894..1903
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1904..2237)
                     /gene="pyrH"
                     /locus_tag="PLK68_09735"
     CDS             complement(<1904..2237)
                     /gene="pyrH"
                     /locus_tag="PLK68_09735"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609730.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRM90722.1"
                     /translation="MKYNRVLLKLSGEALAGDDKTGINAHTVADIARQIKDAKDLGVE
                     IAIVCGGGNLWRGKTGADMGMDRSSADYMGMLATCINALALQDALEKAGVPVRVQTAI
                     EMAEIAEPY"
     gene            complement(2429..>2830)
                     /gene="tsf"
                     /locus_tag="PLK68_09740"
     CDS             complement(2429..>2830)
                     /gene="tsf"
                     /locus_tag="PLK68_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRM90723.1"
                     /translation="NENNEEAGHKVAMQVAAMSPKYVSRDEVSQEYMEHEKEILLAQA
                     KKENPEKPENIIEKMIIGRLNKEMKEICLLDQVYVQDSDLTVAKYVDKVAKENNANVT
                     VKKFVRFETGEGIEKKEENFAEEVAAQMNAK"
BASE COUNT          838 a          572 c          378 g         1032 t
ORIGIN      
        1 tgtatttgta ggcattgata gtggataata agcatttcca tccataataa aagctatatg
       61 ttgaggaata ttatctttat caagaacatg ataattaaat tcttcattct tctttttctt
      121 aaaaaacatt tgtcgtcctc ctgtttaact attcgagatt ataccataaa ttcattacaa
      181 aaaaaagcct aagcaacatt gctagactta atcatcctag tacatataaa ttatttattt
      241 aatttactac attaaaataa gaaaatatac accttcataa cgattatggt ctttacagct
      301 ttttataatt ccagcacctg attgcctgtt ctaatgtctt tcctttgtta tttttaaaga
      361 aatcagaata tatgtattat attcaaattt tttatcaatt acagtttttg ttgacttttt
      421 tgcttctaaa atctcataat atgctttaat tgcatcagat atattttacc agtgttcgtc
      481 ttcaaccatt tttgaaatgg gacgttaaaa gaaaaagttt tgtctatttt ttctttaaag
      541 aaaacacgat gtttttgtga acaaacaaaa ttttcttcaa ttttactatc ttctgttata
      601 attcctattt gacatttact tttattcgaa cttatcaaat ccgaaatatt accagtatct
      661 aaaaaataac caattctctc tgtaatttcc actttaccac cactcatagg taatccatta
      721 actcgacaaa atgccacagg ctcttctttc aaataatagt agctacgaaa tatttcgcta
      781 tctaaatttc aatccgaaca tggtctttta tgcatatatt ttcttctttc taaataaatt
      841 agataaaatg ggcgtttaaa acgcccatct aattatacag tcatgatatc tttttctttt
      901 acttttgcaa tttcatcaat ttctttaaca aatttatctg ttaatttttg aactctttct
      961 tgaccagctt tgatttcgtc ttcactacca tctgttttct taatttcatc attactgtca
     1021 cggcgaatat tgcgaattga caccttagca ttctccgcaa atttccaaac atttttagca
     1081 aactctttac gacgttcttc agttaaagcg ggaaccataa tacgaataat ctcaccatcg
     1141 ttttgtggag taagacctaa atccgcttca taaatgccat gttcaatatc tttaatactt
     1201 gatttgtcat aaggtttaat aactaattga cggccttcta ctaccgaaat accagcaatt
     1261 tgattcactg gagttggtga accataatag ttaaccatta ctcgatcaag catattagga
     1321 tttgcacgac cagttctaac agatgataaa tcacgttgga aagattcaat cgtctttgtc
     1381 attctttcat ttgtatcatc taaaatcata tttatcattt tattttcctc ctgagattgt
     1441 tgttccgata tcatcgccat tacatgcttt agcaatgttt ccgtctacag acatattaaa
     1501 tacacataaa tcaatattat tgtctttaca taaagtgata gctgtttgat ccatgatttt
     1561 taaatctttt tgtaatacat caaaatacga aatagtatcg aagcgaattg cattaggatc
     1621 aacttttgga tcagctgaat aaactccgtc taccccgttt ttagccatta ggataacatc
     1681 tgcgttaatt tctgcagctc tcaaagcagc tgttgtatct gttgtaaaaa aaggacttcc
     1741 tgttccagct ccaaatatta ctactcgccc cttttctaag tgacgaattg ctcgtcttct
     1801 aatatatggt tcagcaactt gacgcatttc aatcgctgaa agaacacgag ttggaacgcc
     1861 aatggcctcc aatgcatttt gaacagctaa accnnnnnnn nnntgtaagg ttctgcgatt
     1921 tcggccattt cgatggctgt ttgtacacgg acaggaacgc ctgctttttc aagtgcatct
     1981 tgcaaagcaa gggcattgat acaagttgca agcattccca tatagtcagc acttgaacga
     2041 tccattccca tatcagcacc agttttacca cgccataggt tcccgccgcc acaaacaatt
     2101 gcaatctcaa ctcctaaatc tttggcatct ttaatttgac gagcgatatc tgctactgta
     2161 tgagcgttga taccagtttt atcatctcca gccaatgctt caccacttaa tttcaataat
     2221 acccgattat acttcatatt aatctcctta tttatatttt ataaaaaaag atttatgctt
     2281 aaattcgcac atcattatca gctaataatg attacttttt cctctaaaaa atttatcata
     2341 ttttcgtcgt ttagtcaagg tacattccta gtcgaatgac gcttatccaa agaaaaaaca
     2401 ctatctaatg atagtgtttt aaaagttatt attttgcgtt catttgagca gcaacttctt
     2461 cagcaaagtt ttcttctttc ttttcgatac cttcaccagt ctcgaaacga acgaacttct
     2521 taactgttac gttagcattg ttttcttttg ctactttgtc aacgtatttt gcaactgtaa
     2581 ggtcgctgtc ctgaacgtat acctggtcta acagacagat ctctttcatc tctttgttga
     2641 gacgtccgat gatcatcttc tcaatgatat tttccggttt ctccgggttc tctttcttag
     2701 cctgagcaag aaggatctct ttttcatgct ccatgtattc ctgagaaact tcatcacggg
     2761 atacatattt cggagacata gcagcaacct gcatagctac cttgtgacca gcctcctcgt
     2821 tgttctcgtt
//
LOCUS       DAOMTA010000362         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1517_length_2829_cov_2.551550, whole genome shotgun sequence.
ACCESSION   DAOMTA010000362 DAOMTA010000000
VERSION     DAOMTA010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_2829_cov_2.551550"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLK68_09745"
     CDS             <1..560
                     /locus_tag="PLK68_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRM90724.1"
                     /translation="HSAGKPVVEQDKCIGCGQCIKICAHNGTSITDHKASIDHDKCVG
                     CGRCIGVCPKDAIVASMDEANDILNYKIAEYTKAVVQDRPCFHISLVIDVSPYCDCHS
                     ENDIAIVPDVGMFASFDPVALDMACADAVNKQPAIANSLLDKHGHHHHDHFTDVSPET
                     NWKSCLEHGEKIGIGTREYELIEIK"
     gene            complement(586..1689)
                     /locus_tag="PLK68_09750"
     CDS             complement(586..1689)
                     /locus_tag="PLK68_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90725.1"
                     /translation="MSIGKKLLSLRQEKGISQEALGRELNVSRQTVSKWESDLSLPDM
                     KMMITISQFYEISITQLLDLDDETEADSINKIYEQTSLVLENLQKENKKKIIRDWIII
                     IICTLCLCVALAILVKKGTNEVVFYPNNSTTPITDNNIVDYSNTTFKVISYDFDKMTM
                     SINYKCVLNNYTDITQVNICIVDVFNNQSIYPMTKENAYTFVLTETIPLVNYANFEIL
                     VKNGDKGEKINADSEIEHHLDNLLSHLIYIYIPGDKNYKLIRNNMIYKLDYSYLENEK
                     IDYSGTLSNKVEIIINKIKNNNYEKIGQTNTTLDKQKKIKLSKDLSNGSEINVIVNII
                     TAEQSYELVNINRYVQTGGISYSEYPIYRYGDI"
     gene            complement(1918..2580)
                     /gene="bioD"
                     /locus_tag="PLK68_09755"
     CDS             complement(1918..2580)
                     /gene="bioD"
                     /locus_tag="PLK68_09755"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRM90726.1"
                     /translation="MIKGLFVTGTGTDVGKTYVSARIVKALKSQYKVGYYKAALSGAV
                     VENDVLIPGDLEVVKQYASLPNESCKVSFVYEEAFAPHLAAKKTNTPVDLNTIKADLT
                     DLENKHDFVVIEGSGGIVCPLRDDKLIMLSDVMLLANYPLIIVTSSGLGSINGAVLTA
                     QYAKQLNLNVLGFIMNNFDSNNLLHQDNRIMIERLSSYKVLGYLNKKADKIEWFEQII
                     ES"
     gene            complement(2577..>2829)
                     /locus_tag="PLK68_09760"
     CDS             complement(2577..>2829)
                     /locus_tag="PLK68_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRM90727.1"
                     /translation="PLEHLKPLSKEDVERIFAIFRFILPDATIRMAGGRGLFDDKGIN
                     VFKSGANGAITGDMLTTQGITIAEDQRIIKQLGFRVAKP"
BASE COUNT          919 a          503 c          359 g         1048 t
ORIGIN      
        1 tgcacagtgc tggcaaacct gtcgttgagc aagataagtg tattggctgt ggacaatgta
       61 ttaaaatttg tgctcataac gggacaagta ttactgatca caaagcctct atcgatcatg
      121 ataaatgtgt tggatgcggt cgctgtattg gagtctgtcc taaagatgca atcgttgcca
      181 gcatggatga agctaatgac attttaaact ataagattgc tgaatatact aaagcagttg
      241 tgcaagatcg accatgtttt catatttcat tggttattga tgtatcacct tattgtgatt
      301 gtcatagtga aaatgatatt gccatcgtcc ctgatgtcgg aatgtttgcc tcatttgatc
      361 ccgttgcttt agatatggct tgtgctgatg ctgttaataa acagcctgct attgcaaata
      421 gtttgttaga taaacatggt catcatcatc atgatcactt tacagacgta tcacctgaaa
      481 caaactggaa atcttgttta gaacatggtg aaaaaattgg aatcggaact cgtgaatacg
      541 aactcattga aataaaatag taaaaaagat cactacgatc tttttttaaa tatctccata
      601 acgatagata ggatactcag aataactaat tcctccagtt tgaacataac ggttaatatt
      661 aaccaattca tatgattgct cagcggttat tatatttacg ataacattta tctcactacc
      721 attagataag tcttttgata attttatctt tttttgctta tctaaagttg tattagtttg
      781 accaattttt tcataattat tatttttaat tttattaata attatctcta ctttatttga
      841 taaagtacca ctataatcaa tcttttcgtt ttctaaataa ctataatcta atttataaat
      901 catattattt ctaatcaatt tataattttt atcacctgga atatagatat aaatcaagtg
      961 actcaacaag ttgtctaaat gatgttctat ttctgaatcc gcattaattt tttctccttt
     1021 atcaccattt ttaaccaaaa tttcaaagtt agcataattc actaaaggaa ttgtttctgt
     1081 taaaacaaaa gtataagcat tttctttagt cattggataa atactctgat tattaaaaac
     1141 atcaacaata cagatattta cttgagttat atctgtataa ttatttaaaa cgcatttata
     1201 attaatactc attgtcattt tatcaaagtc ataactaatc actttaaatg ttgtatttga
     1261 ataatctact atattgttat cggttattgg agttgtacta ttattaggat aaaaaactac
     1321 ctcattagtt ccctttttaa ctagtattgc taatgcaaca cataaacaaa gagtacaaat
     1381 aataataatt atccaatcac gaataatctt ttttttattt tccttttgta gattctctaa
     1441 cactaatgat gtttgttcat atatcttatt gattgaatca gcttctgttt catcatcaag
     1501 atctaataat tgggtaatac ttatttcata aaattgactt attgttatca tcattttcat
     1561 gtctggtaaa cttaaatcac tttcccattt agacactgtt tgccttgata catttaattc
     1621 acggcccaaa gcttcttgtg aaatcccttt ttcttgcctt aggcttagta gttttttacc
     1681 aatgctcata acccatccct cctatctata tcatagatat tttgtctttt ttcgtctata
     1741 tcaatccgtt aacatttcgc caataatgtt aacatgaaaa aaactgtaac tacatattga
     1801 tataactccg ctaaagtaga taggaaccga ttatttataa ttaaaagtga atttgaaata
     1861 tctcccgttt attagatatt aagtattaaa taccgaggta tatttcactt ttttatctta
     1921 actttctata atttgttcaa accattcaat cttatcagct tttttgttta aataccctaa
     1981 aactttataa ctacttaatc tttcaatcat tattcgatta tcttgatgaa ggagattgtt
     2041 actatcaaaa ttattcataa taaaaccaag tacatttaaa tttaactgtt tagcatattg
     2101 agccgttaaa actgctccat taatactacc caagcctgat gatgttacaa ttattagcgg
     2161 atagttagcc agtaacatca catcactaag cattatcaat ttatcatcgc gcagtggaca
     2221 aacaatacca ccacttcctt caataacaac aaaatcatgc ttattttcta aatctgtcaa
     2281 atctgctttt atagtattta aatcaactgg tgtattagtc ttttttgcag ctagatgagg
     2341 cgcaaatgct tcttcataaa caaaagatac tttacagctt tcatttggca gacttgcata
     2401 ctgcttaaca acttctaaat cacctggtat cagtacatca ttttcaacaa cggcaccact
     2461 taaggccgcc ttataataac caaccttata ctgtgatttc aaggctttaa ctattcttgc
     2521 acttacatat gtctttccaa catctgtccc tgttccagta acaaacaaac ctttaatcat
     2581 ggcttggcta ccctaaatcc caattgctta atgattcttt gatcttcagc aatagttatt
     2641 ccctgagttg taagcatatc tccagtgata gcgccattag caccactttt aaatacattt
     2701 attcctttat catcaaaaag ccctctacca ccagccattc taatcgttgc atcaggaaga
     2761 ataaaacgaa aaatggcaaa tattctttca acatcttctt ttgataatgg tttcaaatgt
     2821 tctaacggt
//
LOCUS       DAOMTA010000363         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1522_length_2826_cov_2.495489, whole genome shotgun sequence.
ACCESSION   DAOMTA010000363 DAOMTA010000000
VERSION     DAOMTA010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_2826_cov_2.495489"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLK68_09765"
     CDS             complement(<1..244)
                     /locus_tag="PLK68_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90728.1"
                     /translation="MKNKKTNFIYKSCILIYLISLVYAFYLNWGGKYFSMTIVACLTP
                     FIMPLLMKLLKIEVPQEFYIINIIFVYFASLWGSCLG"
     gene            346..507
                     /locus_tag="PLK68_09770"
     CDS             346..507
                     /locus_tag="PLK68_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90729.1"
                     /translation="MHIFSAVLYAVSANIDALSIGIAYGIKKTHINHQKNFMIAFITM
                     IGTYHWQTF"
     gene            492..851
                     /locus_tag="PLK68_09775"
     CDS             492..851
                     /locus_tag="PLK68_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HRM90730.1"
                     /translation="MADFLGSSLLLLIGIYTVVKSLKECDEIIVTNHLSAVKNISLKE
                     TLVLSLVLTINNVALGIGASITGMPELITSLTTFVCSYVFIGTGQKFKRLNFKGKYAD
                     IISGIILIILGLYELTI"
     gene            916..>1827
                     /locus_tag="PLK68_09780"
     CDS             916..>1827
                     /locus_tag="PLK68_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM90731.1"
                     /translation="MYDIIVVGGGHAGIEAALAPARMNQKTVLVTSNFDNVGSLPCNT
                     SIGGPAKGIIVREIDALGGQMPKTADKTYLQMKMLNTAKGPGVQSLRAQADKKVYPRY
                     MQEVLKKQENLDIIEGMVEDLIVEDNCVKGVILDNGQTIEGRMVILTTGTYLKAEILC
                     GDQKHASGPDQQKESKYLSEKLANLGMRIQRLKTGTPPRVEINSVDYSKTSLQPGTDA
                     NLAFSYQTNEFVPIEEQTPCYLTYTNEKTHQIIRDNLHRSSMYSGIVKGIGPRYCPSI
                     EDKIVKFSDKPQHQIFLEPESAEMDTIY"
     assembly_gap    1828..1854
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1855..2769
                     /locus_tag="PLK68_09785"
     CDS             <1855..2769
                     /locus_tag="PLK68_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM90732.1"
                     /translation="QEEMIRTIPGLENCKILKYAYAIEYDAIDPLQLWPSLETKIIKN
                     LFTAGQINGTSGYEEAAGQGLIAGINATLKNQGKEPLILKRDEAYIGVMIDDLVTKGT
                     DEPYRMLTSRAEYRLLIRHDNADERLMKYGHDVGLVSDQIYQAYLDKMSEIFAEIDRL
                     DTIRFTPKHEINDALEHFGSARLTEGISAKELIKRPELTYEKILPYVEAPKLSEEQRK
                     RVTILIKYKGYIDKARRQADKQVKMEEKKIPADIDYEDISNLALEAKQKLSKIRPLTI
                     GQASRISGINPADISVLLIYLKQKYNEE"
BASE COUNT         1025 a          386 c          535 g          853 t
ORIGIN      
        1 cccctaaaca actcccccac aaacttgcaa aatagacaaa aatgatattg ataatataaa
       61 attcttgagg aacttctatt tttaacaatt tcattaataa aggcataatg aatggggtaa
      121 gacaagcaac aattgtcata ctaaaatatt ttcctcccca gttaagataa aaagcgtaaa
      181 ctagagaaat aagatagatt aaaatacagc ttttataaat aaaattagtt tttttatttt
      241 tcataatttt tcgactcctt attatagtat accataatta ctaaatttat ttcctgatgc
      301 ccagagctaa atggttatta tgcataaatt agatggggtg agtttatgca tatattttca
      361 gcagttctct acgctgtatc cgcgaatatc gatgctctaa gcatcggaat agcttatggg
      421 atcaaaaaaa cacatattaa tcatcaaaaa aattttatga ttgcatttat tacaatgatc
      481 ggtacttatc attggcagac tttttaggaa gcagtctgtt actattaata ggaatctata
      541 ctgttgttaa gtcattgaaa gagtgtgatg agatcattgt tactaatcat ttatcagcag
      601 taaaaaatat ttctttaaaa gaaactttag tcttatcatt ggttttaacc attaataatg
      661 tagctttagg aattggtgca agtattactg gaatgccgga gttgattact tctttaacaa
      721 cctttgtttg tagttatgtt tttattggaa ctggtcaaaa atttaagcga ttaaatttta
      781 agggaaaata cgctgatata atctcaggaa ttattttaat tatccttggt ttgtatgaat
      841 tgacaatcta atgattattg ttttaaaaat aggaaatttg tagtacaata gattaggatt
      901 aaggagggaa atataatgta cgatattata gtagtcggtg gtgggcacgc aggaattgag
      961 gcagcgttgg caccagcgag aatgaatcag aaaacagttt tagttacttc taactttgat
     1021 aatgttggaa gccttccttg taatacatca atcgggggac cagctaaagg aattattgtc
     1081 cgggaaatag atgcattagg aggacaaatg cctaaaactg ctgataaaac ctacttacaa
     1141 atgaaaatgt taaacaccgc taaaggtcca ggggttcaat ctttacgggc tcaagctgat
     1201 aaaaaagtat atcctcgata tatgcaagaa gtattaaaaa aacaggagaa tcttgatatc
     1261 attgagggaa tggtagagga tttgattgtt gaagataatt gtgtaaaagg agttattttg
     1321 gataatggcc aaactattga aggaaggatg gttatcttaa caactggtac atatctaaaa
     1381 gcagagattt tatgtggtga tcaaaagcat gcaagtggtc ctgatcagca gaaagaatca
     1441 aaatatttat cagaaaaatt agctaattta gggatgcgga ttcaacggct taagacggga
     1501 acaccacctc gagtagagat taatagtgtt gattactcaa agacaagttt acagccagga
     1561 actgatgcta atttggcatt tagttatcaa actaatgagt ttgttcctat tgaagaacaa
     1621 acaccatgtt atctgactta cactaatgaa aaaactcatc agattattcg tgataatcta
     1681 catcgttcaa gtatgtacag tggtattgtg aaaggaattg gaccacgcta ttgcccatcg
     1741 attgaagata agattgttaa attttcggat aaaccacaac atcaaatctt cttggaacct
     1801 gaatcagcag agatggatac gatttatnnn nnnnnnnnnn nnnnnnnnnn nnnncaagaa
     1861 gaaatgattc ggactatccc aggattagaa aattgtaaga ttttaaagta tgcttatgca
     1921 attgagtatg atgctataga tccactacaa ttatggccat cattggaaac aaaaataatt
     1981 aaaaatctat ttactgctgg tcaaattaat ggaacaagtg gatatgaaga agcggcaggt
     2041 caaggtttga ttgcaggaat taatgcaact ttaaaaaatc aaggaaaaga gccattgatt
     2101 ttaaaacgcg atgaggctta tattggggta atgattgatg atttagtgac taaaggaact
     2161 gatgagccat accgaatgtt gacaagtaga gctgagtacc gtttattgat tcgtcatgat
     2221 aatgcagatg aaagactaat gaagtatgga catgatgtgg gattagtaag tgatcaaata
     2281 taccaggctt atcttgataa aatgtcagaa atttttgcag aaattgatcg tttggatacg
     2341 attcgtttta ctcctaaaca tgagatcaat gatgctttgg agcattttgg ttcagcacgt
     2401 ttaacggaag gcattagtgc taaagaatta atcaaaaggc cagaattaac atatgaaaaa
     2461 atcttaccat atgttgaagc tccaaaacta agtgaagaac aacgtaaacg ggtgactatt
     2521 ttaattaaat ataagggata tattgataag gctcgacgtc aggctgacaa gcaagtcaaa
     2581 atggaagaaa agaaaattcc ggcagatatt gattatgaag atattagcaa tttagcatta
     2641 gaagcaaagc aaaagttatc aaaaattcgg ccactaacga ttggacaggc atcacgtatt
     2701 tccggaatca atcctgcgga tatatccgtt ttattaatct atttaaaaca gaaatataat
     2761 gaggaataga catgaatgaa ttagaattca ttaaacaatt agaaactaaa ggaattactt
     2821 taaccc
//
LOCUS       DAOMTA010000364         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1532_length_2813_cov_2.196882, whole genome shotgun sequence.
ACCESSION   DAOMTA010000364 DAOMTA010000000
VERSION     DAOMTA010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_2813_cov_2.196882"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLK68_09790"
     CDS             complement(<1..284)
                     /locus_tag="PLK68_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM90733.1"
                     /translation="MQLKVSTDYAIRIVLYIAIKKDIVQSKELSETLGIPQSTVFKIG
                     KKLSDNEIISIATGIQGGFKLKKHPKDISIFSIIDIFEPTIRINSCLEED"
     gene            complement(543..1148)
                     /locus_tag="PLK68_09795"
     CDS             complement(543..1148)
                     /locus_tag="PLK68_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM90734.1"
                     /translation="MNKYAYIRVSTKDQNIDRQKFAMKEIGLGTRQMFIDIQSGKDFN
                     RKGYKKLVKKLKKGDELYIKSIDRLGRDYDEIIEQWRYLAKKKEVEIIVLDFPLLDTR
                     NQVNGITGKFIADLVLQVLSYVAQIERENTKQRQAEGIKAAKEKGVRFGRPQLPYPEN
                     FEEIYLCYKEKQISKRECARRLSTNHTTFSNWVNRYELKKE"
     gene            complement(1394..2722)
                     /locus_tag="PLK68_09800"
     CDS             complement(1394..2722)
                     /locus_tag="PLK68_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM90735.1"
                     /translation="MSKIIVIGANHAGTAAINTILDNYEDDVVVFDGNSNISFLGCGM
                     ALWIGKQIDGPEGLFYSSKELLEEKGAIIYMETIVEHVDYEKKIVYAKGKDGKEYQES
                     YDKLILACGSLPMRPTIPGSDLENVQMVKLYQNAQDVIEKLNNPELKNIVVVGGGYIG
                     VELAEAFRRCDKEVTLIDCADTCLGAYYDHSFTDLMNQNLTEHGIKLAFNQSVLEIKG
                     NQKVESVVTDKGEYAADMVILAVGFKPNNELGKDVLKLYSNGAYLVNKKQETSLKDVY
                     AIGDCATVYDNTIDDVNYIALATNAVRSGIVAAHNVCGQPLESIGVQGSNGICIYDLK
                     MVSTGLTLSKAKKLGFKATSVSYRDLQKPAFIKENQEVMIEIVYDEETRRILGCQMAS
                     KYDISMGIHMFSLAIQEHLTIDKLALLDIFFLPHFNQPYNYITMAGLTAK"
BASE COUNT          897 a          540 c          304 g         1072 t
ORIGIN      
        1 ttatcttcct caagacaact gttaattctt atcgttggct caaatatatc tataatgcta
       61 aaaatactta tatcctttgg gtgttttttt aatttaaatc ctccctgtat ccctgtagca
      121 atgctaatta tttcattatc acttagtttc ttcccgattt taaatactgt actttgggga
      181 atcccaagtg tttctgataa ttctttcgac tggacaatat ccttttttat agcgatatat
      241 agaactattc gtattgcata atcagtagaa accttcaact gcacctcgtt ttcctccttc
      301 ctttctaact taaccgatgt taggttagtt tcaaaatcca gaaaagtaca gctatctttc
      361 cgatggaaaa taaagtagac attattttcc actttatata taataattaa tagaaaaata
      421 cagttatatt tttatgtgtt tctagcataa ttattggaaa taatacacgg aaataaaagt
      481 ctaccatgtt tgtcaatcgt ggataaaaaa atctacctta ttttacaaat acttctattt
      541 ttctattctt tttttaattc ataccgattt acccaattag aaaatgtcgt atgattggta
      601 cttaaccgtc ttgcacactc tcttttgctt atctgttttt ccttataaca caaataaatc
      661 tcttcaaaat tttctggata aggtaattga gggcgtccaa atctgacccc tttttctttt
      721 gcagccttaa tcccttctgc ttgtctttgt tttgtatttt ctctttctat ttgtgcgaca
      781 tacgacaata cctgcaatac taaatcagca atgaattttc ctgtaatacc attaacttga
      841 tttctagtat ctaaaagtgg aaaatctaac acaattatct ccacttcttt cttctttgcc
      901 aaataacgcc attgttcaat aatttcatca taatcacgtc ctaatcgatc aatagattta
      961 atataaagtt catcaccttt ttttagtttt ttcaccaatt tcttataacc ttttcgatta
     1021 aaatccttac cagattgtat atcgatgaac atttgtctag tacctaatcc aatttctttc
     1081 atcgcaaatt tttgtctatc aatattttga tctttggttg atacacggat ataagcgtat
     1141 ttattcattc acattcctcc ttataagaaa ataatactta gagaataaaa aaatgtatat
     1201 atctatttag aaatatacat ttaatcaaat ataaatgaaa ttttataatt acaaacaagt
     1261 attctctatt tatcaacaat attattcatt ttctatacct cataatattg attaacatag
     1321 atacttcagc acaataaaaa tacatccaaa aattgctttt gatgcaaatc attgaaatgt
     1381 atttttgtta tttctattta gcagttaatc cagccattgt tatataatta taaggttgat
     1441 taaaatgtgg caagaagaag atatcaagca atgccaattt atcaattgtc aaatgctctt
     1501 gaatcgctaa tgagaacata tgaattccca tcgaaatatc atattttgat gccatttgac
     1561 accctaaaat ccgccttgtt tcttcatcat aaacaatttc tatcattact tcttgatttt
     1621 cttttataaa tgccggtttt tgaagatcac gatatgatac tgaagtagcc ttaaaaccta
     1681 atttttttgc ttttgataaa gttaaccccg ttgataccat ctttaaatca tagatacaaa
     1741 ttccatttga gccttgaact ccaatacttt ctagtggttg accacaaaca ttatgagcag
     1801 caacaattcc cgaacgaact gcatttgttg ccaatgcgat atagttaaca tcatcaatcg
     1861 tattatcata aacagtcgca caatcgccaa ttgcatacac atcttttaat gatgtttctt
     1921 gttttttgtt tactaaatat gccccgttag aatatagttt aagtacatct tttcctaact
     1981 cattattagg tttaaagcca accgctaaaa taaccatatc tgcagcatat tcacctttat
     2041 ctgttacaac tgattcaact ttttgattac ccttaatttc taaaacagat tgattgaatg
     2101 ccaatttaat tccatgttct gttaaatttt gattcattaa atcagtaaat gaatggtcat
     2161 aatacgctcc aaggcaagta tcagcacaat caattaaagt aacctcttta tcacaacgtc
     2221 taaatgcctc agccagttct acaccaatat agccaccacc aactactaca atgtttttta
     2281 actcaggatt atttaatttt tcaattacat cttgagcatt ttgatataat tttaccattt
     2341 gaacattttc taaatcacta ccaggaatcg ttggacgcat tggtaaagat ccacatgcta
     2401 aaattaattt atcataactt tcttgatact ctttaccgtc cttacctttt gcatagacaa
     2461 tctttttttc atagtcaaca tgttccacta tcgtttccat gtatataatt gcaccttttt
     2521 cttccaataa ttctttactt gaataaaata gtccttctgg tccatcaatt tgttttccga
     2581 tccataatgc cattccacac cctaaaaaac taatattgct gtttccatca aaaacaacta
     2641 cgtcatcttc atagttatct agaatcgtat taatcgcagc tgttcctgca tgatttgccc
     2701 caataacaat aattttactc attttttcag tctccttatt ttttatatta ttatcatacc
     2761 catcatttgt atttatttac aactgatatg caccaaaaaa ttaggaaact ata
//
LOCUS       DAOMTA010000365         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1536_length_2811_cov_1.700653, whole genome shotgun sequence.
ACCESSION   DAOMTA010000365 DAOMTA010000000
VERSION     DAOMTA010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1536_length_2811_cov_1.700653"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..795)
                     /locus_tag="PLK68_09805"
     CDS             complement(106..795)
                     /locus_tag="PLK68_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90736.1"
                     /translation="MNIEHLRDKFDLNELELALVKYLEDNQNNLKDITIRQMASANFT
                     STSAIYRLCNKFGFSGYSDMIYQLSSSNNTQLFSPDDLEQYIPAFIELLNKHRQHNII
                     VFGMGFSAPIADYLQQRLTLNGFYAMNVVHTEMLDIKYQDNSLFIVISNSGITPRLVE
                     IVDNAFKNNIDIISFIANENSNLDQNVTLPIIVGSYNSFTHNNLVPNTFFGQVLIVFE
                     SLLYTYLSSIE"
     gene            846..1106
                     /locus_tag="PLK68_09810"
     CDS             846..1106
                     /locus_tag="PLK68_09810"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90737.1"
                     /translation="MAKTMYDPWSKIITRNGYAGDEVISALQKSIRRSLEEQACMFAY
                     EMYISSPELLEKMWRRLLTISVEDIGMGDPMAAVLVNNLYYL"
     assembly_gap    1113..1122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1123..1449
                     /locus_tag="PLK68_09815"
     CDS             <1123..1449
                     /locus_tag="PLK68_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90738.1"
                     /translation="DQPMYFIHAIRYLCSCQKDRSSDLLKNICIKSFAMGKLPEIPDV
                     ALDKHTVRGKAMGRDSFHFLNEASKVIPQMEVDNDYKERYAKILEEYDPENVSETAFT
                     FNGWQF"
     gene            1465..2742
                     /locus_tag="PLK68_09820"
     CDS             1465..2742
                     /locus_tag="PLK68_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90739.1"
                     /translation="MISKLEKLLGPIANKLAQQRHLQAISSGMMMSLGLIVVGSVFLI
                     IANPPINLDLVDLNTGNIFLKFLISWKQFALANYDVLTLPYNYTMGLVGLISAFGVAY
                     CLAESYKMKASVYGIISMCTFLMVAAPLKDGAITMSYLGADGLFVALIISLISVEISR
                     VVSHRIVFTFPDSVPSAVTNFVNSLMPLALNIIVIYGANLILVALSGKSMPNLIMSFL
                     TPAISGVDNVWMFMGIYLFSNILWMFGINGSSIVFPIVFALGIANTGLNGELVNLGKD
                     PTAIMNLQMFRYALLGGAGNTLGLVLLMCRSKSKHISSIGRLSIVPGICGINEPIMFG
                     APIVLNPILSIPFLVMPCISIGLGYLVQKIGLVSMGYIVDPSFTPFFAQGFLSALDIR
                     NVIFMIVLIIISVGVYYPFFKVYEKNTLANEAE"
BASE COUNT          925 a          342 c          529 g         1005 t
ORIGIN      
        1 cagcatgaat atcattattt atagttaaat taaaacatgt ttcgaatttt gaaacatgtt
       61 tttttatcga gaacatctat ttaattttgt cattataagc gtatattatt ctatagagga
      121 caaatatgta taaaggagtg attcaaaaac aattagaacc tgaccaaaga aggtatttgg
      181 tactaaattg ttatgagtaa atgaattata agaaccaaca ataattggta gtgttacatt
      241 ttgatcaaga ttagaatttt cattggcaat aaaactaatt atatcgatgt tatttttaaa
      301 agcattatcg acaatttcaa ctaatcgggg tgtaattcca gaattagaaa tcacaataaa
      361 tagggaatta tcttgatatt taatatcaag catttcagta tggacaacat tcattgcata
      421 aaatccattt agggttagac gctgttgcaa ataatcagca attggagcag agaatcccat
      481 cccaaaaacg atgatattat gttgacgatg tttatttaat aattcaatga atgctggaat
      541 atattgttca agatcatcag gagaaaaaag ctgggtatta ttcgatgatg acagctgata
      601 gatcatatca ctgtagccac taaaaccaaa tttattgcaa agacgataaa ttgcagaagt
      661 agatgtgaaa ttagcgctgg ccatttgccg aatcgtaata tcttttaaat tattttgatt
      721 atcttcaaga tatttaacta atgctaattc aagttcattt aaatcaaatt tatctcttaa
      781 atgttcgata ttcatattat cacctcaatt agattatgtc acagattaga aaaaggagca
      841 agtttatggc aaaaacgatg tacgacccat ggtcaaagat tattacaaga aatggttatg
      901 ctggagatga ggtaatatca gctttacaga aatcaattcg tcgcagttta gaagaacagg
      961 catgtatgtt cgcctatgaa atgtatattt cttcaccgga attattagaa aaaatgtggc
     1021 gtcgattatt aactatttcg gtagaagata ttggaatggg agatccgatg gctgcagtat
     1081 tagtaaataa tttatattat ctttaaaaaa atnnnnnnnn nngatcaacc aatgtatttt
     1141 atccacgcga ttagatattt atgcagttgt caaaaagatc gttctagtga tttattaaaa
     1201 aatatctgta ttaaatcttt tgcgatggga aaattaccgg aaatacctga tgttgcttta
     1261 gacaagcata cagtaagagg aaaagccatg gggcgtgatt cattccattt cttgaatgaa
     1321 gctagtaaag ttattccaca aatggaggtg gataacgatt ataaagaacg ctatgcaaaa
     1381 attcttgaag aatatgatcc agagaatgtt agtgaaacag cattcacttt taatggttgg
     1441 caattttaat agggggataa aaaattgatt agtaaattag agaaattatt aggaccaatt
     1501 gcaaataaat tggctcagca gcgacatctt caagcaattt catcgggtat gatgatgtca
     1561 ttaggattaa tcgtagtagg gtcagttttc ttaattattg ccaatccgcc aattaattta
     1621 gacttggttg atttgaatac aggaaatatc tttttaaagt ttttaattag ctggaaacaa
     1681 tttgctttag ctaattatga tgttttaact ttaccatata attatacaat gggcttagtt
     1741 ggtttgattt cagcttttgg ggttgcttat tgcttagctg aaagttataa gatgaaagct
     1801 tcggtttatg ggataatttc aatgtgtact tttttaatgg ttgcagctcc attaaaggat
     1861 ggagctatta caatgagtta tttaggtgcg gatggtctat ttgttgcttt aataatttca
     1921 ttaatatcag tagagatttc acgagtagtc agtcatcgaa tagtctttac ttttccagat
     1981 agtgttccta gtgcagtgac taattttgta aatagtttaa tgccattagc gctaaatatc
     2041 attgttattt atggggctaa tttgatttta gtagctttat cagggaaaag tatgccaaat
     2101 ttaattatgt cgtttttaac acctgcaatt tcaggggttg ataatgtttg gatgtttatg
     2161 ggaatttatt tattttcaaa tattctttgg atgtttggaa ttaatggttc ttcaattgta
     2221 tttccaatag tttttgcttt ggggattgcg aatacgggac taaatggtga attagtaaat
     2281 ttaggaaaag atccaacagc aatcatgaat ttacagatgt ttagatatgc tttattaggt
     2341 ggagctggta atactttagg attggtttta ttaatgtgtc gttctaaatc taagcatatt
     2401 agctcgattg gtcgtttaag tattgttcct gggatttgtg gaattaatga gccaatcatg
     2461 tttggggctc cgattgtatt aaatccaatt ttatcgatac cgtttttagt aatgccgtgt
     2521 atttcgattg gtttagggta tttagttcaa aaaattggat tagtttcaat gggatatata
     2581 gttgatccat catttacgcc attttttgct caaggttttt tgtcagcatt ggatattcgt
     2641 aatgtaatct tcatgattgt tttgattatt atttcggttg gagtatatta tccatttttt
     2701 aaagtttatg aaaaaaacac acttgcaaat gaagcagaat aataaagtgg ccgatgccac
     2761 ttttaatatg gaggaaagat gaaaaataat tatgatgttg tggttgttgg a
//
LOCUS       DAOMTA010000366         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1542_length_2804_cov_2.305566, whole genome shotgun sequence.
ACCESSION   DAOMTA010000366 DAOMTA010000000
VERSION     DAOMTA010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_2804_cov_2.305566"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /gene="lon"
                     /locus_tag="PLK68_09825"
     CDS             complement(<1..1700)
                     /gene="lon"
                     /locus_tag="PLK68_09825"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRM90740.1"
                     /translation="MNEQTVLTLPVVCTRGMIVFPENRLTLDVGRPVSLKALELSANE
                     HDNNIIFVSQINPLVDNPSFDDVFHIGTLCKIDRKVRRDSSGTIKLTVLGAKRVRLTN
                     FEEQQGSIYSTVEIIEDEFGDRNEEVALVRKTTSYFEQAKRSMPNMPLDSINRLTSGV
                     SASVLADTIGQYLPIDLNQKQKILETININERLLLVASSIESEKVIGEIEETINRKVR
                     ESIDENQREYYLREKLRAIKEELGDSVPKEDDAEMIREELEKNPYPQHVKDKIEEELR
                     RFETMPAASSEANVVRTYIDWMMKTPWYQETKDVEDIQAIEDVLNEDHYGLEKVKERI
                     VEYLAVKQMTKSLKAPIICLAGPPGVGKTSIAKSIARALQREFIKASLGGVKDEAEVR
                     GHRRTYLGSMPGRIIQGMKKAGVINPVFLLDEIDKMSSDYKGDPTSAMLEVLDPEQNN
                     SFYDNYLELEYDLSKVLFIATANDLGNIPAPLRDRLEIIELSSYTEQEKLMIAKNHLI
                     KKQLALHGLKEDQLVIEDDAIMAIIRHYTREAGVRDLERLLAKICRKAVLIVLKEKRD
                     "
     gene            complement(1755..>2804)
                     /gene="tig"
                     /locus_tag="PLK68_09830"
     CDS             complement(1755..>2804)
                     /gene="tig"
                     /locus_tag="PLK68_09830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRM90741.1"
                     /translation="VEILTEANVEPVAQPALSVQKVDADELEVQILVPVKPQVELGEY
                     KGLEVKKGRVTVTKKEIEEQLANYQTQFAELTVKEGGKVAKGEVIIDFEGFVDGVAFE
                     GGKGENYPLEIGSGSFIPGFEDQVIGMTVDKEQDIVVTFPEDYGAADLAGKEATFKVT
                     VHEIKEKHLPEIDDELAKDVNIDGVETLDQLKDHIKANIKTRKESENENKFMDDLYKA
                     IVASSKVEDSDALLEQEQGLMLQEIEQNLQRQGLNFEVYQQFTGKSKDDIKEDIKPQA
                     EERVKLNAILAAIIEEEKLAVSDEELETELKTIAEYYQKELDEVKKIFEGNMSRIEND
                     LLTRKAVDLVKDNLK"
BASE COUNT          796 a          575 c          366 g         1067 t
ORIGIN      
        1 ttatcacgtt tttcttttaa aacgattaat accgctttac gacaaatctt tgccagcaat
       61 ctctcaagat cacgaactcc agcttcgcgc gtatagtgac gaataatcgc cataattgca
      121 tcatcttcaa ttactagttg atcttccttt aaaccgtgta aagctaactg ctttttaatt
      181 aaatgatttt tagcgatcat tagtttttct tgttctgtat atgaagatag ttcaatgatt
      241 tccaaacgat ctctaagcgg agctggaata tttcctagat catttgcagt agcaataaac
      301 aaaactttgc tcaaatcata ttccagttct aaataattat catagaaact attgttttgt
      361 tcagggtcta aaacttcaag cattgcacta gtcggatcac ctttatagtc acttgacatc
      421 ttatcgattt catcaagtaa gaagaccgga tttattactc ccgctttctt catcccttga
      481 ataattcttc ctggcataga cccaagataa gtacgacgat gtccacgtac ttcagcttca
      541 tctttaactc cacctaatga tgccttgatg aattcacgct gtagtgcacg ggcaatactt
      601 ttagcgattg aagttttccc aacccctggt ggcccagcaa gacaaataat tggtgctttt
      661 agcgatttag tcatttgttt aaccgctaag tattcaacaa ttcgttcctt taccttttca
      721 agaccataat gatcttcatt taaaacatct tcaattgctt gaatatcttc aacatcttta
      781 gtttcttgat accaaggtgt tttcatcatc cagtcaatat atgttcttac tacattagct
      841 tctgaagaag ctgctggcat tgtttcaaaa cggcgtaatt cttcttcaat tttatcctta
      901 acatgctgtg gataaggatt tttttcaagt tcttcacgaa tcatttctgc atcgtcttcc
      961 ttcggtacac tatcacctaa ttcctcttta atagcccgaa gtttttctct taaatagtac
     1021 tcacgttgat tttcatcaat actttcacga actttacgat taatcgtttc ttcaatttca
     1081 cctatcactt tttccgattc aattgaacta gctactaata ataaacgttc attgatatta
     1141 atagtttcta agattttttg tttttgatta agatcaattg gtaaatattg tccaattgtg
     1201 tcagctaaaa cacttgctga tactccacta gttaatcgat tgattgaatc tagcggcata
     1261 tttggcatac tgcgttttgc ttgttcaaaa tacgaagttg tcttacgaac taaagcaact
     1321 tcttcatttc gatctccaaa ttcatcttca ataatttcaa cagttgagta gatacttcct
     1381 tgttgttctt caaagttagt cagcctaact ctttttgccc ccaaaacagt taatttgata
     1441 gttcccgaac tgtcacggcg cactttacga tcaattttac aaagagttcc aatatggaac
     1501 acatcatcaa aagaaggatt atccactaat ggattgattt gtgagacaaa aatgatattg
     1561 ttatcatgtt cattagcact taattcaagt gcctttaaac tcactggacg accaacatct
     1621 aatgttaacc tgttttctgg gaaaacaatc atgccccgtg tacaaactac cggtaacgtt
     1681 aatacagttt gttcattcat tctatttcct cctatttcgc tattttacta aaaagacgca
     1741 tgagcgtctt ttcattattt taagttgtct tttactaaat caacagcttt acgagttaac
     1801 aaatcgtttt cgatacggct catatttcct tcgaaaattt tcttaacttc gtctaattct
     1861 ttttgataat attcagcaat tgtttttaat tctgtttcta attcttcatc actaactgct
     1921 aatttttctt cttcgataat tgctgcaaga atagcattta atttcactct ttcttcagct
     1981 tgaggtttaa tatcttcttt gatatcatct tttgatttac cagtaaattg ttggtaaact
     2041 tcaaaattta acccttgtct ttgtaaattt tgttcaattt cttgtaacat taatccttgt
     2101 tcttgttcta ataaagcatc actgtcttct actttagaag acgctacgat tgctttatat
     2161 aaatcatcca tgaatttatt ttcattttca gattctttac gagttttaat attagctttg
     2221 atatgatcct tcaattgatc taaagtttca acaccatcaa tatttacatc tttagctaat
     2281 tcatcatcaa tttctggtaa atgtttttct ttgatttcat gtacagtcac tttgaaagta
     2341 gcttctttac cagccaaatc agctgcacca taatcttctg ggaaagtaac aacgatatct
     2401 tgttctttat ctacagtcat tccaatcact tgatcttcaa atcctggaat aaatgaacca
     2461 ctaccgattt ctaatggata gttttcacct tttccacctt caaaagcaac accgtctaca
     2521 aatccttcaa agtcgataat tacttcacct ttagcaactt tcccaccttc ttttactgtt
     2581 aattcagcaa attgagtttg atagtttgct aattgttctt cgatttcttt tttagttact
     2641 gtaacgcgac cttttttaac ttctaatcct ttatattcac caagctcaac ttgtggtttt
     2701 actggtacta agatttgaac ttccaattca tcagcatcta ctttttgaac acttaaagca
     2761 ggttgtgcta ctggttcaac atttgcttca gttaaaattt caac
//
LOCUS       DAOMTA010000367         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1543_length_2804_cov_2.208439, whole genome shotgun sequence.
ACCESSION   DAOMTA010000367 DAOMTA010000000
VERSION     DAOMTA010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1543_length_2804_cov_2.208439"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1704
                     /gene="mgtE"
                     /locus_tag="PLK68_09835"
     CDS             361..1704
                     /gene="mgtE"
                     /locus_tag="PLK68_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRM90742.1"
                     /translation="MIDTITSDELKDILLNGTPETIKKVITNIHPADILDILHEDEDS
                     IKALMDNLPNDVVASIIEEEDDEDDQYDLLKLFSDAKQKEILDEMSNDEITDLIGELE
                     EDEKQAILDKMDKEDKEDVERLLTFEPDTAGGIMTTEYISIRARNTVEKTLKFLQENT
                     EEDTTYYLYVVDPQNILKGVVSLRDIVTSSFDTAMLDITNTNVKTVLYNEDQEEVAKK
                     FQKYGFIMMPVVDEMDHLLGVIEFDDIIDIIQEESTEDINLLGGVNSEERLDSSVGES
                     VKSRIPWLIVNLFTAVMAASVVSFFEGTIAQVVTLATVMPIVTGMGGNAGTQSLTIVV
                     RGLSLGEMSKENATWIMLKEVAVGFCSGVIIGIIVALGSMLFEGNPVFGLVTGLAMFL
                     NMILANIAGYFIPVILEKFHIDPALASGVFVTTVTDVLGFFFFLGLATVFLPYLI"
     gene            1716..>2804
                     /locus_tag="PLK68_09840"
     CDS             1716..>2804
                     /locus_tag="PLK68_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90743.1"
                     /translation="MEVKYVKKNIAKIIISLFFIIMMLFGAAFALIWFKHIFNYMTCL
                     IVVIVVFIVIIILNKKGQICRNVEIGEQGLVDHLGTLEYREIKKVIYDNNRIEMNYGF
                     ANIVTKYHEVLLDKLQEQGVTMIEYNGRLINFERVSLLIIYGLGIYFFYNLFVLIFGI
                     ISCLNYNYEIINSLGIVVRVLKLISILLALFVYNRLKNKKATVIIIIVIIAGFIVWGQ
                     SVKIKTYHDYYGKFACIMKERELNIYKDIIAEYGILDKTINNLDSYKVADLTNYEKVI
                     VYQGTNISGQYIVHRDVKNNNSKEQIFAYYNGKTFTHGALRITFNDGKLAIIDHNGAV
                     YDYDKLKLVNGYILEIYKDNEVKKCLIFN"
BASE COUNT          984 a          315 c          542 g          963 t
ORIGIN      
        1 tcaatacttg agataacaga tttttataaa agcaaaatac aattaactaa ataatctgtc
       61 atttttattt aagagcaatg atatttgtag tgtcctaaaa agttagggta actttaggcg
      121 gtcactacaa tcggctcttt ttctttttag ttcagcaaaa agaaaaagaa tttcagaatc
      181 ttaaattact atttaatttt attcaaaaag tcccctataa tcgcataatt gtgttaaaat
      241 gacttggaga aagaaggtgg taaaaaatga aagtcgagcc tgtaccccag tgttgcgata
      301 gtctagagga tcatttacca ctaagatcag gtatctaatc cccaataaag gaggaaactt
      361 atgatcgata caattaccag cgatgagctg aaggatattc ttttaaatgg aacgccagaa
      421 acaataaaaa aagtaataac taatattcat ccggctgata ttttagatat tcttcatgaa
      481 gacgaggata gtattaaagc attaatggac aatcttccta atgatgttgt tgccagcatt
      541 attgaagagg aagatgatga agatgaccag tatgatttac tgaaattatt ttcagatgct
      601 aaacaaaaag aaatcttaga tgaaatgtct aatgatgaaa tcactgattt gatcggtgaa
      661 cttgaagaag atgaaaaaca ggctatctta gataagatgg acaaagaaga taaagaagat
      721 gttgagcgac tgttaacgtt tgagcctgat acggccggtg ggatcatgac gacggaatat
      781 attagtattc gtgcgcgcaa taccgttgaa aaaactttaa aatttcttca agaaaatacc
      841 gaagaagata caacttatta tctttatgta gttgatccac aaaatatttt aaagggtgtt
      901 gtatctttac gagatattgt tactagctcg tttgatacag ctatgctgga tattactaat
      961 acaaatgtaa aaactgtatt atataatgaa gatcaggaag aagtagcaaa aaaatttcaa
     1021 aaatatggat ttatcatgat gccggtagtt gatgaaatgg accatttatt aggagttatc
     1081 gagtttgacg atattattga tattattcaa gaagaaagta cagaagatat taatttatta
     1141 gggggagtaa attctgaaga aagactagat tcatctgttg gagaatcggt caagagtcgg
     1201 attccctggt tgattgttaa tttatttacg gcagtaatgg cggcttccgt ggttagcttt
     1261 tttgaaggaa cgattgctca agtagttaca cttgcaactg ttatgccaat agttacagga
     1321 atgggcggta atgcaggaac tcagtcttta acaatcgttg tacgagggct ttcattaggt
     1381 gaaatgagta aagagaatgc aacctggatc atgttaaaag aagtagctgt tggtttttgc
     1441 agtggtgtga taataggaat tatagttgcg ttaggctcaa tgttatttga aggaaatcca
     1501 gtttttggtt tagtaacggg attagccatg tttttaaata tgattttagc aaatattgcg
     1561 ggttatttta ttccggttat tttggaaaaa tttcatattg atccagctct agcaagcgga
     1621 gttttcgtca caactgtaac tgatgtattg ggatttttct ttttcttagg attggcaaca
     1681 gtatttttgc catatttaat ttgaggcaat taagtatgga agtaaaatat gtgaaaaaga
     1741 atattgctaa aatcataatt agtctatttt tcattattat gatgctgttt ggagcagctt
     1801 ttgcattgat ttggtttaaa catattttta attatatgac atgtttgatt gttgtgattg
     1861 tggtttttat tgtaattatt attcttaata aaaaaggaca aatttgtcgc aatgttgaaa
     1921 ttggtgaaca agggttagtt gatcatttgg gaaccttgga gtatcgcgag ataaaaaaag
     1981 ttatttatga taataataga attgaaatga attatggttt tgctaatatt gtaacaaaat
     2041 accatgaagt tctgctcgat aaacttcaag aacaaggcgt tacaatgatt gaatataatg
     2101 gtcgtcttat taattttgaa agagtttcac ttttaattat ttatggttta gggatatatt
     2161 tcttttataa tttatttgtt ttaatttttg gaataatttc ttgcttaaac tataactatg
     2221 agattattaa tagtcttggc atagttgttc gagttttgaa attaataagt attttgctag
     2281 cactatttgt atataaccgg ttaaaaaata aaaaagcaac agtgatcatt ataattgtaa
     2341 ttattgcagg atttattgta tggggacagt ctgttaaaat aaaaacatac catgattatt
     2401 atgggaagtt tgcttgtatc atgaaagaac gtgagttaaa tatttataaa gatattatcg
     2461 cagaatatgg aattttagat aaaacgatta ataatttaga tagttataaa gttgcagatt
     2521 taaccaatta tgaaaaagta attgtgtatc aaggaacgaa tatatcagga cagtatattg
     2581 tccatcgaga tgtaaaaaat aataattcaa aagagcagat ctttgcttat tataatggga
     2641 aaacttttac tcacggagct ttgcgtataa cttttaatga tggtaaacta gctattatag
     2701 atcacaatgg agcggtttat gattatgata aactaaagct ggttaatggt tatattctcg
     2761 aaatatataa agataatgaa gtgaaaaagt gtttaatttt taat
//
LOCUS       DAOMTA010000368         2793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1549_length_2793_cov_2.556245, whole genome shotgun sequence.
ACCESSION   DAOMTA010000368 DAOMTA010000000
VERSION     DAOMTA010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1549_length_2793_cov_2.556245"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLK68_09845"
     CDS             complement(<1..852)
                     /locus_tag="PLK68_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90744.1"
                     /translation="MKKLYRLPEGFKFGSSASAWQTEGWTGKKEHQRNFVDMMYLAEP
                     ERWFNGVGTIKSTDFYNRYEEDIKLMKELGIQVYRTSIDWSRFITDYETNEVDQDALR
                     FYEKVIDCLIENGIEPMLCLEHWEIPEYVINKYGTWDNRKVMELFVGYSKKVMAAFHD
                     KIKYWWTINEPAVVPNEAYLFGNIWPYEENTKKSVQMNYHRVLATSLLVEHHAKMGYQ
                     GKVGIILNPSPSYPKSMNNPKDIEAAKICDMFNYKQYTDPLINGKFDEAYFELLKACI
                     NVPSYVIF"
     assembly_gap    942..951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<952..2141)
                     /locus_tag="PLK68_09850"
     CDS             complement(<952..2141)
                     /locus_tag="PLK68_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90745.1"
                     /translation="MDKFSKFLEEKLMPIGQKINNIRFLQVIRMAMMPLIPFIIAGSF
                     TLIVLNFPFIDKVLPAGFLEMLGNVLSPLSGTTLSLVAMFLAFLMGYNYAKTEEQKCE
                     EVYAGITTLVAFLIVSPLSITVGEEVIGGVIPTTYMGSQGMFVALILCYLVAKVYCKI
                     LSSKLKIKLPDSVPPMVSSSFESLIPVIITLVLVCSINYCFTLTSFGNIHALVNEIIQ
                     KPLLLIGTGLPALLISQGLAQLLWFFGLHGDQIVGSVMDPILQTAGMENLSAYTAGDA
                     VPYIITDQFRALFVMIAFMSLVIAILIVSKSNRLKGVGKVAVLPATFCISEPVVFGAP
                     VVMNAMLFIPWVLSRPVFGLITWLFMKFGLCPYPTGVAIPWTTPPVLSGFLATNSIMG
                     AVVQ"
     gene            complement(2257..>2793)
                     /locus_tag="PLK68_09855"
     CDS             complement(2257..>2793)
                     /locus_tag="PLK68_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM90746.1"
                     /translation="NEGYIERRRGKGTIVLEKENKVETILKSSFNGVLEKNDIKNRRV
                     LNVEMVNAGQEIADFFNITTKDKVLRLVREIRINNMVVSIHETFLNPIVPLDEQGDYS
                     GSLYEKLMGAGYGISNVKEKISASLMNQKQKELFEIKGDEAMINRQRRGFCHDFPVEF
                     TNSMYLSQGYELIIDLCE"
BASE COUNT          938 a          545 c          335 g          965 t
ORIGIN      
        1 aaatatgaca tacgaaggaa catttataca agcttttaat aattcaaaat atgcttcatc
       61 aaactttcca tttatcaaag ggtcagtata ttgtttataa ttaaacatat cacaaatttt
      121 tgctgcttca atatctttag gattattcat actttttgga taagatggtg acggatttaa
      181 gattattcct acttttccct gataacccat ttttgcatgg tgctcgacta ataaagaagt
      241 tgcaagaaca cgatgataat tcatttgtac tgatttttta gtattctctt cataaggcca
      301 aatatttcca aataaatatg cttcgttagg aactaccgca ggttcattaa tagtccacca
      361 atatttaatc ttatcatgaa aagccgccat tactttttta ctataaccta caaacaattc
      421 cataacctta cgattatccc atgtcccata cttattaata acatactcag gaatttccca
      481 gtgttctaaa caaagcattg gctcaattcc attttcaatt aaacaatcaa ttaccttttc
      541 ataaaatctt aatgcatctt gatcaacttc attagtttca taatctgtaa taaagcgtga
      601 ccaatctatt gaagtccgat atacttgaat tcctaattct ttcattaatt taatatcttc
      661 ttcataacga ttataaaagt ctgtagactt gattgttcca actccattaa accatctttc
      721 tggttcagct aaatacatca tatcgacaaa attacgttga tgttcttttt tccctgtcca
      781 tccctctgtt tgccaagcgg aagcagaact tccaaattta aatccttctg gtaatctata
      841 taactttttc attttattgc tccttcgttt taatttcttc ttgatgatac gttttgtcaa
      901 tcattttaac aaatggaatg aacatcaaaa ctccttttgc tnnnnnnnnn natttgtaca
      961 actgctccca tgatcgagtt agttgctaaa aatcctgata aaactggtgg tgttgtccat
     1021 ggtattgcta ctcccgttgg atatggacat aatccaaatt tcataaatag ccaggttatc
     1081 agaccaaaca ctggtcttga taaaacccat ggaataaata gcattgcatt catgaccact
     1141 ggtgctccaa atactacggg ttctgaaata caaaaagttg ctggtaaaac tgctaccttt
     1201 ccaactcctt ttaatcggtt gctttttgat acgattaaga ttgcaataac taatgacata
     1261 aaagcaatca taacaaataa tgctctaaat tgatcagtaa ttatatatgg aacagcatca
     1321 ccagcagtat aagcgctcaa attttccatt cctgctgttt gaagaattgg atccattacg
     1381 gaaccaacaa tttgatctcc atgtaatcca aaaaaccaaa gcaactgagc aagaccttga
     1441 gaaatcaata acgctggtaa tccagtacca attaataaaa gtggtttttg aataatttca
     1501 ttgactagag catgaatatt gccaaatgat gttaaagtaa aacaataatt gatactgcaa
     1561 actaaaacta atgtaataat tacaggaatt aacgattcaa aagaacttga taccattggt
     1621 ggtacactat ccggtaactt tatttttagt ttactactta aaatcttgca ataaactttg
     1681 gccaccaagt aacataaaat caatgctaca aacattcctt gtgaacccat ataagtagtt
     1741 ggaataactc ctccgataac ttcttcaccc actgttattg ataatggtga aacaatcaaa
     1801 aaagctacta aagttgtaat tcccgcgtat acttcctcac atttttgttc ttctgtttta
     1861 gcatagttat accccattaa aaatgctaaa aacattgcca ctaaacttaa agttgtccct
     1921 gataatggtg ataaaacatt tcccagcatt tctaaaaagc ccgctggtaa aaccttatca
     1981 ataaatggga aatttaatac aattaatgta aatgacccgg caataataaa tggtattaac
     2041 ggcatcattg ccattctaat aacttgaaga aatctaatgt tattaatttt ctgcccaatt
     2101 ggcattagct tttcttccaa aaacttgctg aacttgtcca tttctcttcc tcctcttcaa
     2161 caaatgttat aactttatac cattatgata atatacttta tttattatgt caatatattt
     2221 agctaaaata aaaaaatgtg tcaaaatgac acatccctac tcacataaat caataattaa
     2281 ttcataccct tgtgaaagat acatagaatt agtaaactca actggaaagt catgacaaaa
     2341 tcctcgcctt tgcctattaa tcattgcttc atcaccttta atttcaaata gttctttctg
     2401 tttttgattc attaagctag cacttatttt ttcctttaca ttactaattc cataccctgc
     2461 acccataagt ttttcataca gagaaccact ataatctccc tgttcatcta aagggacaat
     2521 aggatttaaa aaagtctcgt gaattgaaac aaccatatta ttaatacgga tttctctaac
     2581 taatcgtaat actttatctt tagttgtaat attaaaaaaa tctgcaattt cttgaccagc
     2641 atttaccatt tcaacattta aaacccgacg attctttata tcattttttt ctaacacacc
     2701 attaaatgaa gattttagaa tagtctctac tttgttttcc ttttctaaaa caatcgtccc
     2761 ttttcctctt cgtcgctcta tatagccttc att
//
LOCUS       DAOMTA010000369         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1553_length_2787_cov_2.594436, whole genome shotgun sequence.
ACCESSION   DAOMTA010000369 DAOMTA010000000
VERSION     DAOMTA010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1553_length_2787_cov_2.594436"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLK68_09860"
     CDS             <1..456
                     /locus_tag="PLK68_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90747.1"
                     /translation="LFYYNDHSNNGYGNSHEDYMISNTDNYALFSGVPELCEFHGNNQ
                     QVTALNIGISLNVLNNVEIALTALQGVLNFNSFDQSPKVSFNLSALPSAIKELEITLE
                     DGSLLKADNIELIATSNGEHSCLVLTFVDSYTNTSRWEILLSPIMENVK"
     gene            611..1084
                     /locus_tag="PLK68_09865"
     CDS             611..1084
                     /locus_tag="PLK68_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90748.1"
                     /translation="MSFNFPTTDELNEQGFDKQFLSALTHVKKHMDDDSNTPIHFGAF
                     IYFWERYLFAHADRLSENYKGGSWTLKNGFWCLDSSDQFDVHTGYGAKYTVNAMEFSI
                     IVNLFALSHMAITTYQQKGNEFINMLSVNFSDYIKLLLDRSLEKLNTEAIYMVTD"
     gene            1312..1596
                     /locus_tag="PLK68_09870"
     CDS             1312..1596
                     /locus_tag="PLK68_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90749.1"
                     /translation="MKKIKIEVTDNYEGSEMNKGCFDVEISSKVAGVERKIKAFVNEK
                     NHVSVRSGVWIHSLFKNAPFTSEYVQKANVAIQESFKNWNNSITELNVNI"
     gene            1641..2021
                     /locus_tag="PLK68_09875"
     CDS             1641..2021
                     /locus_tag="PLK68_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90750.1"
                     /translation="MKSLKFNVVDEKHSKDIQNEIFNLGGGWEVSGSTDISQEYKYTD
                     KPFIHVVASYKFDQFGDITDEKYLRLTYSEKERYEYNFADHVDEKTARNAQSDLSIKY
                     EVTLDNFREYLHKIGFRSKKENIK"
     gene            2124..2306
                     /locus_tag="PLK68_09880"
     CDS             2124..2306
                     /locus_tag="PLK68_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90751.1"
                     /translation="MASTAAERQKERHNRMLKKGFKKRAFYVNEDTIKALTSYREANK
                     LESLDEALQQILKTLI"
     gene            complement(2397..2690)
                     /locus_tag="PLK68_09885"
     CDS             complement(2397..2690)
                     /locus_tag="PLK68_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90752.1"
                     /translation="MEFEVQAKSNNANDIDIFLPVESLDKAFEVAEQQKTVENCYVFI
                     VRKGSKPNDYPFQLHKWQRCEYSGINEWEETNSFTIFLGKYPQYDPETGKSCS"
BASE COUNT          975 a          420 c          506 g          886 t
ORIGIN      
        1 ttattttatt ataatgatca ctcaaacaac ggatatggca acagccatga ggattatatg
       61 atttcaaata ctgataatta tgcccttttc tctggtgtac ctgaactatg tgaatttcat
      121 ggaaataacc aacaggtaac agctttgaat attggaatta gcctaaacgt attaaataat
      181 gtggaaattg cgctaacagc attacaaggc gtattaaatt tcaatagttt tgatcagtca
      241 ccaaaggtaa gttttaacct gtcagccttg cctagtgcta ttaaagagtt agaaattacg
      301 ttggaggatg gtagtctttt aaaagctgat aatattgaac ttattgccac aagtaatgga
      361 gaacatagtt gccttgttct tacctttgtc gatagctata caaatacttc acgatgggaa
      421 attttactaa gccctattat ggagaacgtc aaatgagcta tgggcaatac tgtacaggaa
      481 atatcaaaat aaatttctga tccgaaatta ttttgtatta tgttgtaaag cgctattgac
      541 aggatagcgc ttattttatt ataatgatca ctcaaacaac ggatatggca acagccatga
      601 ggatttgagt atgtctttta atttccctac tactgatgaa ttgaatgagc aaggctttga
      661 taagcaattc ttgagtgcat taactcacgt aaaaaaacac atggatgatg attcaaatac
      721 tcctatacat tttggtgctt ttatatactt ttgggaacgc tacttatttg ctcatgcaga
      781 tcggttaagt gaaaactata aaggcggttc atggacactt aaaaatgggt tttggtgttt
      841 agatagtagt gatcagtttg atgtacatac tggatacgga gcgaaatata cagttaatgc
      901 tatggaattt tcaataattg taaatttatt cgcattgtct cacatggcta tcacgacata
      961 ccagcagaaa ggaaatgagt ttataaacat gctttcagtt aatttttcag actacattaa
     1021 gctactgtta gatcgaagtt tggaaaaact aaataccgaa gcaatttata tggttaccga
     1081 ctaaatcagc ataagcaagg cgaaagcctt gcttttttca ttagcattga ttgttctttt
     1141 tgttttgaaa taagggctat tgtgtgtaat ttaccgttca tcggcagaat aaaaaataat
     1201 taaagtaatg cttgactgtg agattctcac attatataat taactcatca agacgagatg
     1261 gctagatcga aagtcttgat gaacttaaac attacttagt gagatgaaat tatgaaaaaa
     1321 atcaaaatag aagttacaga caattatgaa ggttctgaaa tgaacaaagg ctgctttgat
     1381 gtggaaatta gctcaaaagt tgcaggggtg gaaagaaaaa ttaaggcatt tgttaatgaa
     1441 aaaaatcatg tttctgttcg ttcaggcgtt tggattcatt cattgtttaa aaatgccccg
     1501 ttcacttctg aatatgttca gaaagctaac gttgcgattc aagaatcatt taaaaactgg
     1561 aataattcaa taactgaatt aaatgtaaat atctaaaaga aagtattgat aaattaaaca
     1621 ttaattagtg agatatagat atgaaatctt taaaattcaa tgtggttgat gagaagcata
     1681 gtaaagatat tcaaaatgaa atttttaatt taggtggtgg atgggaggtt tcaggcagta
     1741 ccgatatatc acaagaatat aaatacactg ataaaccatt tattcatgtt gtagccagct
     1801 ataaatttga ccaatttggg gatattacgg acgaaaaata cttacgttta acgtactcag
     1861 aaaaagaaag gtatgagtac aattttgcag atcacgttga tgaaaaaacg gctcgaaatg
     1921 cccaatctga tttgagcatt aagtatgaag taaccctcga taattttaga gaatatcttc
     1981 ataaaatcgg ttttaggtca aaaaaagaga atattaagta aagcaaaaac cccactggct
     2041 agagtggggt tttcttctgt ctatgtgaca gtaaacatta cttagcaagc taagtccgat
     2101 taatatatca gtgagtttaa aaattggcaa gcacagcagc agaacgccaa aaagaacgtc
     2161 ataatcgtat gcttaaaaaa ggattcaaaa aaagagcttt ttatgtgaat gaagatacga
     2221 taaaagcatt aacgtcttat agagaagcaa acaaactaga aagccttgac gaagccttac
     2281 aacaaatatt aaaaaccctc atttgagggt tttcttaatt gtgcatttgt cagttccaat
     2341 ttactaatca atgttgcgac aagcccaaca atctacattc aactatgcga cataatttat
     2401 gaacaggatt tgccagtttc gggatcatat tgagggtatt tacccaaaaa aatcgtaaag
     2461 ctgtttgttt cctcccattc attaataccg ctatattcac atctttgcca tttatgcagt
     2521 tggaatgggt agtcgtttgg tttagagcct tttctgacga taaatacata acaattttca
     2581 acagtctttt gttgttcagc aacttcaaaa gctttatcta agctctctac aggcagaaaa
     2641 atatcaatgt cattagcatt gtttgacttg gcttgtactt caaattccat aattaatttc
     2701 cttaatcgca aagtagtttg gaatgtcgca tagttattta gaactcacag cacttttaaa
     2761 gcatcacgaa gcgtattatg tgcgctt
//
LOCUS       DAOMTA010000370         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1557_length_2783_cov_2.590909, whole genome shotgun sequence.
ACCESSION   DAOMTA010000370 DAOMTA010000000
VERSION     DAOMTA010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_2783_cov_2.590909"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..947)
                     /locus_tag="PLK68_09890"
     CDS             complement(57..947)
                     /locus_tag="PLK68_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM90753.1"
                     /translation="MYYIGIDGGGTKTAFGLFDECGTCLYRVNYPTCHFLQVGFDGCA
                     QLIKKGIEDVIKHHPDCSKELQIGIGIAGYGNDKAVCEQLESHIEKELKGYRYVLTND
                     MHIALIGALDGQDGIAVVAGTGSIAMAHIHSKIYRTGGWGYQLGDEGSAYWIGKKLLN
                     EFCKQADYRQPRDELYDRILDYFKIENPYEIISAIHDMNNERTEIAALAKMCGVLADK
                     GHAICQDILKQAGYHISQLVQGLLPHFEKEVQVTYYGGVFQNIIFKQAFCYQLLDCQF
                     TEPIHNALVGAYIFAKKSHF"
     gene            complement(947..2041)
                     /locus_tag="PLK68_09895"
     CDS             complement(947..2041)
                     /locus_tag="PLK68_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HRM90754.1"
                     /translation="MKKLGISLYVEKSNLEEMKTYIDKAQQAGFTRIFSCLLSVNESA
                     DKIKSDFSIINQYAHEKGFEIFVDVSPKVFDKLNISYQDLTFFKEIGADGIRLDAGFS
                     GNEEALMTFNPQGLKIEINMSNDTHYIDTIMDYCPNRSQLVGCHNFYPHRYSGLSLEH
                     FLKSTQNFKKYGLTTAAFVSTNQSDAYGPWPTKEGLPTLELHRELSLEVQIKHMIALD
                     VIDNIIISNCYPSDEELKNISHMRLDLVTFNVELVNSLPETERKIVLEELHFNRGDYN
                     DNIIRSTQSRVKYKGHEFKLFNVPDYISPGDIIIESSEYGHYAGELQIAKTRMKNSGK
                     SNVVGHIVEAERFLLSYIKPWQKFTFYEVK"
     gene            complement(2054..>2783)
                     /locus_tag="PLK68_09900"
     CDS             complement(2054..>2783)
                     /locus_tag="PLK68_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90755.1"
                     /translation="TTRYYADGLPKNTSTNAAVQPYKYNGKEFITMHGLDTYDYGARG
                     YYPAYMKRFFFEHNIDISITPSDKELLLQNTSDFIAFSYYSSSVVAFEEAHQTAGNLV
                     ATIKNPYLKATKWGWQIDPIGIRVSLNRFYNRYQKPIIIAENGLGNQDTLEMDKTIHD
                     TYRIKYLQEHFHQIQEAQKDGVDIIAYCLWGIIDIVSAGSCEMEKRYGVIYVDADNKG
                     RGSYCRYKKDSFYWYQQFIEKYHQ"
BASE COUNT          893 a          570 c          367 g          953 t
ORIGIN      
        1 tactggtttg tccaataaag agggtacata tcattatcca aattatatgg ttcacttcaa
       61 aaatgactct tttttgcaaa aatatatgca ccaacaagag cattatgaat cggttcagtg
      121 aattggcaat ctagcaactg ataacaaaac gcctgtttaa aaataatatt ttgaaaaact
      181 ccaccataat aagtcacttg tacctctttt tcaaaatgcg gtagtaatcc ctgaaccaat
      241 tgtgaaatat gataaccggc ctgttttagg atatcttgac atattgcatg tcccttatca
      301 gcaaggacac cacacatctt tgctagagct gcaatctctg ttctttcatt attcatatca
      361 tgaatagcag atataatttc ataaggattt tcaatcttaa aataatctaa gattcgatca
      421 tacaactcat ctcttggttg acggtaatct gcctgtttac agaactcatt taataatttt
      481 ttacctatcc aataggctga tccctcatca ccaagctgat agccccatcc accagttcga
      541 taaatcttcg agtgaatgtg cgccatcgcg attgaccctg ttcctgctac tacagcaatc
      601 ccatcttgtc catcaagtgc accaatcaat gcaatgtgca tatcattggt taaaacataa
      661 cgatatccct tcagttcttt ttcaatatga gattccaact gttcacaaac tgccttatca
      721 tttccatatc cagcaattcc aataccgatt tgcagttctt tagaacagtc aggatgatgt
      781 tttataacat cttctatacc tttttttata agttgggcac atccatcaaa accaacctgt
      841 aaaaaatgac aagttggata atttacccgg tacaaacacg ttccacactc atcaaataat
      901 ccaaacgcag ttttcgttcc gccaccatca attcctatat aatacattac ttcacctcat
      961 aaaatgtaaa tttttgccat ggttttatat atgataataa aaatctttca gcttcaacta
     1021 tatgaccaac aacattactt tttccactat tcttcattcg tgtttttgca atttgtaatt
     1081 cacctgcata atgcccatac tcagaacttt caataataat atcaccggga gaaatataat
     1141 cagggacatt gaaaagttta aattcatgtc ctttatattt aacacgactc tgcgtagaac
     1201 gaattatatt atcattataa tctccacgat taaaatgtaa ttcttctaaa acaatctttc
     1261 tttcagtttc gggtaaagaa ttaacaagtt caacattaaa tgtaacaaga tctaatcgca
     1321 tatgcgaaat attttttagt tcctcatcac ttggataaca attagaaatg ataatattat
     1381 ctattacatc taacgctatc atatgtttaa tttgaacctc cagagataat tcacggtgca
     1441 attctaatgt aggcaacccc tcttttgtgg gccatggtcc ataagcatca gactgatttg
     1501 ttgaaacaaa tgcagcagtt gttaatccat atttcttgaa gttttgagta gattttaaga
     1561 aatgttctaa agataaacca ctatagcgat gcggataaaa attatgacat ccaacaagtt
     1621 gacttcgatt tggacaataa tccataatag tatcaatata atgtgtatcg ttgctcatat
     1681 taatttcaat ctttaatcct tgtggattaa aagtcattaa cgcttcttca ttcccgctaa
     1741 atccagcatc caaacgaatg ccatctgcgc ctatctcctt aaaaaaggtt aaatcttgat
     1801 agctaatgtt taatttatca aatactttag gactcacatc tacaaaaatt tcaaacccct
     1861 tttcatgagc atactgatta ataattgaaa aatcagattt tatcttatct gcgctttcat
     1921 ttactgataa tagacatgaa aagatacgtg tgaaacctgc ttgctgagcc ttatctatat
     1981 atgtcttcat ttcctccaga ttagattttt ctacatagag cgaaattcct aattttttca
     2041 tatctgtttc ctcctattga tgatactttt caataaactg ctgataccaa taaaaactat
     2101 cttttttata tcgacaataa cttcctcttc ctttattatc tgcatcaaca tagataactc
     2161 catacctttt ttccatttcg caacttcctg cagatacaat atcaataatt ccccataaac
     2221 aataagctat aatatcaact ccatctttct gtgcttcttg aatctgatga aaatgttctt
     2281 gcaagtattt gattcgatat gtatcatgaa tagttttatc catttccaac gtatcttgat
     2341 ttcccaatcc attttctgca ataataattg gcttttgata acgattatag aaccggttta
     2401 atgacactct tattccaatc ggatcaattt gccatcccca ttttgtagcc tttaaatatg
     2461 gatttttaat tgtagccaca agatttccag cagtttgatg agcctcctca aatgcaacta
     2521 cacttgatga ataatatgaa aacgcaataa aatcagaagt gttctgcaat aatagttctt
     2581 tatccgatgg cgttatactg atatctatat tgtgttcaaa aaagaaacgt ttcatataag
     2641 caggataata ccctctggct ccataatcgt aggtatccaa accatgcatc gttataaatt
     2701 cctttccatt gtacttatac ggctgcacag cggcattcgt actggtgttt ttgggtaagc
     2761 catcggcgta atatcgcgtt gtc
//
LOCUS       DAOMTA010000371         2774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1564_length_2774_cov_2.297168, whole genome shotgun sequence.
ACCESSION   DAOMTA010000371 DAOMTA010000000
VERSION     DAOMTA010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1564_length_2774_cov_2.297168"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLK68_09905"
     CDS             complement(<1..1060)
                     /locus_tag="PLK68_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90756.1"
                     /translation="MIDKLTMFFEQKLSGPMEKLAQQRHLRAVRDGIVATLPLIIVGS
                     FFLIIAMPPLPQDWEIYQFLNGNAATIMLPYRMTMFIMTIYATFGIGYSLAESYKLDR
                     LTGGILAMIAFLCTITPVSVSAEAAEVAGVSGWVMPMANLGGGGLFVSIIVSCLAVEV
                     YRLTSKSKFKITMPAQVPPAVARSFEALTPTIVVIVGMACITYFIGFDWHGAVAAVVS
                     PLVKAADSLPSVLLLIFLITGFWSFGIHGVSIIGSLARPLWLQLIDANTAMAAAGQAP
                     TAIAAEPFFQWFIWIGGSGCTIGLAILMAFRAKSKYASSLGKTCIVPSLFNINEPLIF
                     GTPIVLNPILIIPFIVVPM"
     gene            complement(1080..1433)
                     /locus_tag="PLK68_09910"
     CDS             complement(1080..1433)
                     /locus_tag="PLK68_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90757.1"
                     /translation="MAPKVVTSNYKKVKKNKKVDGNISPIPKFSYKSAVIIVLSAALS
                     CFFLPNMIAGFGVDTRITTLIGNAVIISFAVCYCRYFIETDKGFHKNFIIVYFCFFIA
                     IGIISYFWLYIGLYM"
     gene            complement(1450..2358)
                     /gene="murQ"
                     /locus_tag="PLK68_09915"
     CDS             complement(1450..2358)
                     /gene="murQ"
                     /locus_tag="PLK68_09915"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRM90758.1"
                     /translation="MPNDLSLLMTEQQNMNTLHIDEMDTIEILNTINQEDKNVALAVR
                     NELVAISRAVDAIYKKVARGGRLIYIGAGTSGRLGVLDASECPPTYGVSPTLVQGIIA
                     GGTDALLNAIEGAEDSQELAKEDLEKIELTEHDVVCGLAASGRTPYVIGGIKYANLIG
                     ATTLSICCVSKGLISNFAQYPIEVKVGPEVITGSTRMKAGTAQKMVLNMLSTSVMIKL
                     GKVYGNLMIDVQPTNEKLKKRAIGIVEKCTGLASIEAKKLLESTGYDVKCAILMEKTK
                     LDFESCRNLLLKYQNNVTKVIRQYKK"
     gene            2576..>2774
                     /locus_tag="PLK68_09920"
     CDS             2576..>2774
                     /locus_tag="PLK68_09920"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRM90759.1"
                     /translation="MYGIGIMSGTSLDGIDTVLVKITGYSTNTKLEVIAYNEYPLHNE
                     LVAKIKNACSLEHSHSDLICSL"
BASE COUNT          919 a          559 c          396 g          900 t
ORIGIN      
        1 ccatcggaac aacaataaac ggaatgatta atattggatt caatacaatc ggtgtcccaa
       61 aaataagagg ttcattaata ttaaataaag atggaacaat acatgtcttt cctaaagaac
      121 ttgcatattt agattttgca cggaatgcca ttaaaatagc aagtccaatt gtacatcctg
      181 aaccaccaat ccagataaac cactgaaaaa aaggttctgc tgctatagcc gtaggtgctt
      241 gacctgctgc cgccattgct gtatttgcat caattaactg taaccataac ggtcttgcta
      301 aagaacctat aatagaaacc ccatggattc caaaagacca gaatcccgta atcaagaaaa
      361 taagcaatag gacacttggc aaactgtctg ctgcttttac aagcggtgat acaacagctg
      421 caactgctcc atgccagtca aatccaataa aataagtaat acatgccatt cccacaataa
      481 caacaatcgt tggtgttaat gcttcaaaag aacgtgctac agctggcgga acctgagcag
      541 gcatagtaat tttaaatttt gattttgatg ttaaacgata aacttccacc gctaagcatg
      601 atacaatgat ggaaacaaac aatccgcccc cacctaaatt agccattggc ataacccatc
      661 cagaaacacc agccacttct gccgcttctg ccgatacaga cacaggtgtg atcgtacata
      721 agaaagcaat catcgctaaa atcccaccag ttaatctatc tagtttataa gattcagcta
      781 aactgtatcc aattccaaaa gtcgcataaa ttgtcataat aaacattgtc atacgatatg
      841 gaagcataat tgtagcagca ttcccattta aaaattggta aatttcccaa tcttgcggaa
      901 gaggcggcat tgcaataatc aagaagaatg aacctacaat gattagtggc agagttgcca
      961 caataccatc tcgaactgca cgcaaatgtc tttgttgtgc taatttttcc ataggtccag
     1021 ataatttttg ttcaaaaaac attgttaatt tatctatcat gaacttaatc ccctttcatt
     1081 tacatataaa gcccaatata caaccagaaa taactgataa ttccgattgc tataaaaaaa
     1141 caaaaataca ctataataaa gtttttatga aaccccttat cagtttctat aaaataccgg
     1201 caataacaga ctgcaaaact tataataaca gcatttccta tcaatgttgt gattcttgtg
     1261 tctaccccaa aacctgcaat catattgggg aggaaaaagc aagagagtgc agcacttaaa
     1321 acaataatga cagcactttt ataactgaat tttggtattg gtgatatatt gccatcaacc
     1381 tttttatttt tcttcacttt tttataattt gatgttacaa cttttggcgc catctgaatc
     1441 actccttcat tatttcttat actgtcttat aactttagtg acattattct ggtactttaa
     1501 caataaattc cggcacgatt caaaatctaa tttagttttt tccattaata ttgcacattt
     1561 gacatcgtac ccagttgact ctaataattt tttcgcttct attgatgcaa gtcctgtaca
     1621 cttttctact ataccaattg ctcttttctt taatttctca tttgtaggtt gaacatcaat
     1681 cattaaatta ccatagactt tcccaagttt gatcatgact gaagtagata acatattgag
     1741 caccatcttc tgtgcagttc ctgccttcat tcttgttgag ccagtgatga cttcaggccc
     1801 aacttttact tcgattggat attgtgcaaa atttgatatt aaccccttac taacacaaca
     1861 gattgatagt gttgtggccc caattaaatt tgcatactta atcccaccta taacatatgg
     1921 tgtacgtcct gaagctgcca aaccacaaac aacatcatgt tcagttaatt cgattttttc
     1981 taaatcttct ttggcaagtt cttgagagtc ttccgcacct tctattgcat ttaatagtgc
     2041 atcagttcct ccagcaataa taccctgcac cagtgttggt gaaactccgt aagtcggtgg
     2101 acattctgaa gcatccagca cccctaaacg tccagaagtt cccgcaccga tataaataag
     2161 tctaccacca cgtgcaactt ttttataaat tgcatctaca gctctggaaa tagcaacaag
     2221 ttcatttctc acagccaatg caacgttttt gtcttcctga ttaatcgtat ttaaaatctc
     2281 gatggtatcc atttcatcta tatgcaaagt attcatattc tgttgttcgg tcatcagcaa
     2341 acttaaatca tttggcatcc acttcccctc ctttcaatct tattctaaac tatttaaaat
     2401 aatatttcaa tatttttatt atttatttgt taatttattt cagtatatat aatttttata
     2461 agaaatttta tttcttaatt gttaataaat atgttttttt aaacaatata cttatattaa
     2521 tgttgaaatt ttatttcgat aataatacaa tatttatgaa caaggaggta tatttatgta
     2581 tggtataggt attatgtcag gaacatcatt agatggaatt gatacagttc ttgtaaaaat
     2641 aactggatat agcacaaata caaaactgga ggttatcgct tataacgaat accctcttca
     2701 taatgaactt gttgcaaaaa taaaaaatgc atgttcctta gagcactctc atagtgatct
     2761 catttgcagt ttga
//
LOCUS       DAOMTA010000372         2766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1577_length_2766_cov_2.037993, whole genome shotgun sequence.
ACCESSION   DAOMTA010000372 DAOMTA010000000
VERSION     DAOMTA010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_2766_cov_2.037993"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PLK68_09925"
     CDS             complement(<1..1036)
                     /locus_tag="PLK68_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM90760.1"
                     /translation="MKVKKTRRQFYDSIYILAIKHGLMLAIPFLILGSFALLFNSFPI
                     PDYQVFLAAFLDGGLKDGLSIIYNCSLGSIALILIITISLSYGKLNGQDDVFFYPITA
                     IISYLSFCGGLHEKTYIFQSDWVFTAMCITLLTCAMLKKGMHLVNHLEKLYTVGTNYI
                     FNRAIQGIFPIVFIIIIFTSAGQIMKGLLGDINIVNFGSYFFIYLFSWFGNGIIGTLL
                     YVFFVHFLWFFGIHGTNTLDMVAKQLFEPGVQINQALIQNGQLPTELFSKTFLDIFVF
                     IGGCGTALCLILAIFIAAKKSNNKKLAKVAGISVFFNINEIVIFGFPVIFNPIMLIPF
                     ILTPLVLTIIS"
     gene            complement(1469..1666)
                     /locus_tag="PLK68_09930"
     CDS             complement(1469..1666)
                     /locus_tag="PLK68_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90761.1"
                     /translation="MSENYIRNHFDDIKKYGNVIENRLDDENCTLDSVLKDNAQFSKL
                     AQKSKMNYILIDDPYKIDIDL"
     gene            complement(1745..1879)
                     /locus_tag="PLK68_09935"
     CDS             complement(1745..1879)
                     /locus_tag="PLK68_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90762.1"
                     /translation="MGLIRSGNTELTPMSDNDALTKYLWPIVCEMIKTVIENKQSLII
                     "
     gene            complement(1992..>2766)
                     /locus_tag="PLK68_09940"
     CDS             complement(1992..>2766)
                     /locus_tag="PLK68_09940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM90763.1"
                     /translation="ELRSINYGDIDMKNYYGSLCTEMYEILHKDAPEDELDFYLSYTR
                     KDMSILEPLCGSGRFLVPFLERGYDIKGIDLSKEMLAKLKEKSPNAKVFQSDILEYDT
                     VEKYDYIFISSGSVSLFTDMVLCRKILKKMKWLLKKGGKFVFAVDTVANRCPDDSDYR
                     TAIAIKTKERYDLILKNKNYYDEKTHTQFSPGIYELYKGAELLQQEIMDFQTHLYELG
                     ELEQHLQDIGFTSVTVYSSYEKIIAKNDHTEMFLYECSF"
BASE COUNT         1003 a          488 c          306 g          969 t
ORIGIN      
        1 tactgattat tgttaaaact aacggtgtca aaataaaagg aatcagcata ataggattaa
       61 aaataacagg aaaaccaaaa ataacaattt cattaatgtt gaaaaaaaca gatatacctg
      121 caaccttcgc aagtttctta ttattacttt ttttagcagc aataaagatt gctaaaatta
      181 agcataatgc tgttccacaa ccgccaataa aaacaaatat atctagaaat gtttttgaaa
      241 ataattctgt tggtaattga ccattttgta ttaacgcttg attaatttgt actcccggct
      301 caaacaactg cttagcaacc atatccagtg tattagtccc atgtattcca aaaaaccata
      361 aaaaatgtac aaaaaataca tataacaagg tcccaataat tccattacca aaccagctaa
      421 acaaatatat aaagaaatat gagccaaaat taacaatatt gatatcacct aataaacctt
      481 tcataatctg cccagcactc gtaaatataa taatgataaa gactatcggg aaaattcctt
      541 gtattgctcg attaaaaata taatttgtcc ctaccgtata caatttttct aaatgattta
      601 caaggtgcat tccctttttt aacatcgcac aagttaaaag ggttatgcac attgccgtaa
      661 agacccagtc agattgaaaa atatatgtct tttcatgcaa tccgccacaa aaagaaaggt
      721 atgaaattat tgctgtaata ggataaaaaa agacatcatc ttgaccattt aattttccgt
      781 atgaaagtga gatagtaata attaaaatta acgcaataga acctaaggaa cagttataaa
      841 taatgcttaa tccgtctttt aagcctccat ctagaaaagc cgctaaaaag acttgataat
      901 cggggattgg aaagctatta aacaacaaag caaaagagcc aagaatgagg aatggtattg
      961 ctagcattaa tccgtgttta attgccaaaa tataaatcga gtcataaaat tgccgtcttg
     1021 ttttcttaac tttcatattc ccatccctct acttaattac taccgtaaga cataaaatca
     1081 atatattata atctatcaaa taacaataaa tctgttgtcc caaactagtt aaatagacct
     1141 gaattatttt tacaaagtaa ttaccaggca catcccctta tctaatccat aattattctt
     1201 ttagtttttc tctcttattt aaaaaatgtg cttattaaaa ttttcaatca tgagccagta
     1261 ttgtctaact cctttattca cttaaactaa tattttctct catctataca ttaatttttt
     1321 ttctatttat ctaccatttc tataattata tcaagtagca aaaatttagt aaatgacctt
     1381 cactaaataa accattaaaa atagaacaat aaaaaattta agaatagtat aaatattaat
     1441 ttgattctta tagcataaac aataattatt ataaatcaat atctatttta tatggatcgt
     1501 caataagtat gtaattcatc ttactttttt gggcaagttt agaaaactga gcattatcct
     1561 tcaacacact atccagagta caattttcat catctagacg attttcaatg acattaccat
     1621 attttttgat gtcatcaaaa tgatttctaa tataattttc actcattata agacaataat
     1681 accttatatt atccaaatat ttttttgtga aatttttctt ccagtcaaaa ggaatataac
     1741 atctttaaat aataaggctt tgcttatttt caataacggt cttaatcatt tcacaaacaa
     1801 ttggccataa atatttagtt agagcatcat tatcactcat aggtgttaat tcagtgtttc
     1861 cgctacgaat tagccccatt ttcagatgat catggaaatt tatacttttc aagcagcttt
     1921 tgagcaagcg ctgtcttacc tgtatgcgaa gctcctgtga ttaagactat catctttatc
     1981 catccctttt tttaaaatga acactcatac aagaacattt ctgtatgatc atttttagca
     2041 attatttttt catacgatga atacactgtt acacttgtaa atcctatatc ctgtaaatgt
     2101 tgttctagtt cacccaattc atataaatgt gtctgaaaat ccataatttc ttgttgcaac
     2161 agttctgctc ctttatatag ttcataaata cctggagaaa actgggtatg cgttttttca
     2221 tcgtaatagt ttttattttt aagaatcaaa tcataccttt ctttagtctt tatagctatt
     2281 gcagtccgat aatcagaatc atcaggacat ctattcgcta ctgtatcaac tgcaaaaaca
     2341 aatttgccac ctttttttaa caaccacttc atttttttca aaatttttct acataatacc
     2401 atatctgtaa ataatgatac agaaccagaa gaaataaaga tgtaatcata tttttctaca
     2461 gtatcatact ccagtatatc actttgaaac actttagcat taggtgactt ttcctttaat
     2521 tttgcaagca tttctttaga taagtctatt cccttaatat catatcctct ttctagaaat
     2581 ggaactaaaa atcttccact accacataaa ggttctaaaa ttgacatatc ttttctagta
     2641 taagatagat aaaaatctaa ttcgtcttct ggtgcatcct tatgcaagat ctcatacatt
     2701 tctgtacaca aactaccata ataatttttc atatctatat ccccataatt tatacttctt
     2761 aattct
//
LOCUS       DAOMTA010000373         2762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1580_length_2762_cov_2.361286, whole genome shotgun sequence.
ACCESSION   DAOMTA010000373 DAOMTA010000000
VERSION     DAOMTA010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1580_length_2762_cov_2.361286"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLK68_09945"
     CDS             complement(<1..567)
                     /locus_tag="PLK68_09945"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRM90764.1"
                     /translation="MKVVDMHCDTILRLYDEGGCLSKNDFNIDLKKMVKGDYLLQNFA
                     MFVNLSDHLDPLTKAQRLIDLYYTELEKSPELIKPVYSYEDILNNQKNGLMSAMLTLE
                     EGAVVNNDLAILRNYYRLGVRMITLTWNHPNGIGYPNLISTKEFKDLYHLNTEDGLTD
                     FGIAYVKEMERLGIIIDVSHLSDAGFYDV"
     gene            complement(579..>1149)
                     /locus_tag="PLK68_09950"
     CDS             complement(579..>1149)
                     /locus_tag="PLK68_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM90765.1"
                     /translation="TWSGFNLVKNQLEMNNMYIYDSRQVGAPVMDMALAAKQMADNDH
                     DVNEIFEMLEMKTKNSFSFLAPFDFKQLARSGRLSPVAAKMASILKVKALLYLKEDGS
                     CVDKYAMSRTESKIVKTVIDKFKNAGVNAKDYTLYISHADNEESALKAKRLLQDIFDN
                     IEVIVNKLPAVLTCHGGMKCISVHYIHKM"
     assembly_gap    1150..1182
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1183..1448)
                     /locus_tag="PLK68_09955"
     CDS             complement(<1183..1448)
                     /locus_tag="PLK68_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM90766.1"
                     /translation="MKNYLVVTDTTSAMNGVQAKESGIELVSLSVLIDGIEYKDQIDI
                     STEELYKKLEAGCIPTTSQPNTGYLHELMANWKAKNYEAIIIIT"
     gene            complement(1463..1723)
                     /locus_tag="PLK68_09960"
     CDS             complement(1463..1723)
                     /locus_tag="PLK68_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90767.1"
                     /translation="MAATEKEKATKLAHELVAYIESEQSDLKASDNEFDALWQSIYDV
                     CMLVHFNILDELLSEEEYLEGVTWLKDYQHLTKDYQDKELEL"
     gene            1790..2026
                     /locus_tag="PLK68_09965"
     CDS             1790..2026
                     /locus_tag="PLK68_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90768.1"
                     /translation="MKTISIFQVQDINNLLKERQFDYILKLRDACGSQSLYLECTGEE
                     TDINILCNVINELLKNDYLQVIPGTINPYNLLLK"
     gene            2170..>2762
                     /locus_tag="PLK68_09970"
     CDS             2170..>2762
                     /locus_tag="PLK68_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM90769.1"
                     /translation="MAKRPVKIATIGGGSSYTPELVEGFIKRYDELPIKELWLVDIEE
                     GKEKLEIVGAMAQRMVKAAGVPMTIHLTLDREEALKDADFVTTQFRVGLLDARIKDER
                     IPLSHGIIGQETNGAGGMFKAFRTVPVILEIVEDMKRLCPNAWLVNFTNPSGMVTEAV
                     IKYGKWDKVVGLCNVPISCRKMVGKALDKNEEELFFK"
BASE COUNT          918 a          497 c          387 g          927 t
ORIGIN      
        1 tacatcataa aatcccgcat cactcaaatg tgaaacatcg ataatgattc ccaaccgctc
       61 catttccttg acataagcaa tcccaaaatc agttaatcca tcttctgtat ttaaatgata
      121 taaatcttta aattccttag tactaatcaa attaggataa cctattccgt taggatgatt
      181 ccaagttaaa gtaatcatcc taacgcctag acgataataa ttacgtaaga ttgcaagatc
      241 attatttacg actgcccctt cttccaatgt aagcatcgct gacattagac catttttttg
      301 attattaaga atatcttcat aactatatac tggtttaatc agctcaggac ttttttctag
      361 ttctgtataa tatagatcaa ttaatcgctg tgcctttgtt agcggatcaa gatgatcact
      421 taagtttaca aacatcgcaa aattttgcaa caaataatca cctttaacca ttttctttaa
      481 atcaatatta aaatcatttt ttgataaaca gccaccttca tcataaagcc gcaagatggt
      541 atcacaatgc atatcaacaa ctttcatttt ctcacctatt acatcttatg tatataatgc
      601 acagatatac atttcatccc gccatgacaa gtcaatacag ctggtaactt gtttacgata
      661 acctcaatat tatcaaaaat atcttgtaat aatcgttttg ctttcagcgc tgattcttca
      721 ttatcagcat gagagatgta taatgtataa tccttagcat taacaccagc atttttaaat
      781 ttgtcaatta cagttttaac aatttttgat tctgtcctac tcatcgcata tttatcgaca
      841 caactaccat cttccttcaa ataaagtaaa gcttttactt tcaagattga tgccatttta
      901 gcagctaccg gcgataaccg tcccgagcgc gccaattgct taaaatcaaa tggggctaaa
      961 aaagaaaaac tatttttagt tttcatttct aacatttcaa aaatttcatt tacatcatga
     1021 tcattatcag ccatctgttt agccgctaat gccatatcca tgacaggtgc accaacttgg
     1081 cggctatcat aaatatacat attattcatt tctaactgat ttttcacaag attaaatcca
     1141 ctccaagttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaggtgat aatgataata
     1201 gcttcataat ttttagcttt ccaattcgcc attaattcat gtaaatatcc agtatttggc
     1261 tgtgatgtcg tagggataca tccagcttcc agttttttat ataattcttc cgttgaaata
     1321 tcaatttggt ctttgtactc gatcccgtca atcagtacac ttagtgagac taactcaatt
     1381 ccagattctt tcgcttgaac cccgttcatc gcacttgttg tatcggtaac aactaaataa
     1441 tttttcatta ttctttatcc tttcataatt ccaattcttt atcttggtaa tctttagtga
     1501 gatgttgata gtcttttagc caagtaactc cttctaaata ttcctcttca ctcaagagtt
     1561 catctaaaat gttaaagtga actagcatac aaacatcata aatgctttgc cataaagcat
     1621 caaattcatt atcactagcc ttcaaatcac tttgttcact ctctatgtat gcgactaact
     1681 catgagccag cttggttgct ttttcttttt cagttgcagc cattatatat cctccttgac
     1741 ttttatataa tgaaattata acattatttt aacaaaagga gttaaaaaca tgaaaacaat
     1801 ttcaattttc caagtccaag acatcaataa tttattaaaa gaacgccaat ttgactacat
     1861 tctcaaatta cgtgatgctt gtggcagtca aagtctctat cttgaatgta ctggtgaaga
     1921 aaccgatatt aatatattat gcaatgtaat caatgaatta ttaaaaaatg attatttaca
     1981 agtaattccc ggaactatca atccatacaa cctgttatta aaataatata ttaattatta
     2041 ccataagcac gataacttat tatattattt tcataaaatg aaagcggttt tcatcacctg
     2101 aaaaaagacc actttctttt cttttgccgt tataatagcc ttgtaacaaa aaacaaagga
     2161 gatttacaaa tggcaaaaag acctgtaaaa attgctacca tcggtggcgg gagttcatat
     2221 actccagaat tagttgaagg atttattaaa agatatgatg aactacctat caaagaactg
     2281 tggttagttg atattgaaga aggtaaagaa aaattagaaa tcgttggagc aatggcacaa
     2341 agaatggtaa aagctgcggg tgttcctatg acgattcatt taacattaga tcgtgaagaa
     2401 gctttaaaag acgctgattt cgttactact caatttagag ttggcttatt ggatgcaaga
     2461 attaaagacg aaagaattcc cttaagtcat gggattattg ggcaagaaac aaatggtgcc
     2521 ggaggaatgt ttaaagcatt tagaactgtt cctgttatct tagaaatcgt tgaagatatg
     2581 aaaagactat gtccaaatgc ctggctagta aactttacta atcctagtgg aatggttact
     2641 gaagctgtta tcaaatatgg taaatgggat aaagttgttg gtttatgtaa tgtacctatc
     2701 tcttgccgta aaatggttgg aaaagcttta gataaaaacg aagaagaatt attctttaaa
     2761 tt
//
LOCUS       DAOMTA010000374         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1584_length_2759_cov_2.379068, whole genome shotgun sequence.
ACCESSION   DAOMTA010000374 DAOMTA010000000
VERSION     DAOMTA010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1584_length_2759_cov_2.379068"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1926)
                     /locus_tag="PLK68_09975"
     CDS             complement(94..1926)
                     /locus_tag="PLK68_09975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HRM90770.1"
                     /translation="MKYEELARFIIKNVGGEKNINIVQHCATRLRFELKDEEKVNKVN
                     LENNSGILQVLFSGGQCQVVIGTHVADVYKAILATAKIGTVKEQSNKNKKLLDKIFAL
                     FSGIFTPFVPVMAGAGVLRGLLTIFTSMGILTTESGTYMILYAAADAILYFMPFYLAI
                     SASRYFKIDEFIGVTLAGTMLYPTLLTAFSEGAKLSFLGLDVILIKYASSVVPIIVAV
                     YAASLLDKLIRDRLPSIIRSFVTPLIDLIIMAPLSLLAIGPVVTFLTNVFTDGLLAVY
                     SFNPIIFGFIFCACWQPLVIFGLHRGFIPVNLNNLATTGRDALLAMTMPSAFAQTGAA
                     LGIAFKSKNKNFKSVAAANVIPSVLGITEPLIYGTTLKAKKAFFMACLMSGFGGAVVG
                     GAGCYTTGLPAGGILSLPLFAEHGFVWYIAGLLISFIGTLVLVLLFWKDEFVEKNDDV
                     IKETDQLLEVDTEILVAVGTGKVLPLEIVNDSTFSSLALGNGVAIIPDEGKLYAPCDA
                     KIVAVFPTNHAIGLRSILGAEILLHVGIDTVKMEGKGFYAFVKEGDMVKKGQLLLDFN
                     IDEINHAGYDPTIIVIVTNTNHYNKVELLNMNTVVNNDDILRLE"
     gene            complement(2017..2232)
                     /locus_tag="PLK68_09980"
     CDS             complement(2017..2232)
                     /locus_tag="PLK68_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM90771.1"
                     /translation="MTHLKFFAKRLMFGEKEAKRDDILFDIVIERYPEAFECVKNIEK
                     HVQKIYKKDLSQAEKLYLTLHIARLKY"
     gene            complement(2247..>2759)
                     /locus_tag="PLK68_09985"
     CDS             complement(2247..>2759)
                     /locus_tag="PLK68_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM90772.1"
                     /translation="MGRGIAYQKSRGEVVPDEMIEKQFYLKNDKQNNRFLELIKDIPV
                     NVLNVTDEIIDFARKGLDKELNDGIYITLTDHINYAIERYLQGINVQNPLLWEIRQFY
                     QEEYKIAAEALKIINQKLMVNLSEDEVGFIALHFVNAELNSEMEEVTQITKIIQEILN
                     IVRYHLMRIL"
BASE COUNT          943 a          560 c          311 g          945 t
ORIGIN      
        1 gattggcagc acttgcacca ccccacagaa aatctttatc taattcacac ataccgaaca
       61 cctttccttt ctctattatt gatttatgat ctcttactcc aatcgcaaaa tatcatcatt
      121 atttactact gtattcatat ttaacagctc gactttatta taatgattag tatttgttac
      181 aataacaata atagttggat catatcccgc atggttgatt tcatcaatgt taaaatctag
      241 taataattgt cctttcttga ccatatcacc ttcttttaca aaagcataaa aaccttttcc
      301 ctccatttta actgtatcaa ttcctacatg taataatatt tctgctccta atattgacct
      361 taacccaatt gcatgattag tcggaaatac agcaacgatc ttagcatcac agggagcata
      421 taatttccct tcatcaggaa taatagcaac cccatttcct aatgctaacg aactaaaagt
      481 actgtcattt actatttcta acggcaatac tttaccagtc cctacagcaa ctaatatctc
      541 ggtatcaacc tcaagcagtt gatcagtttc cttgattaca tcatcatttt tttcaacaaa
      601 ttcatctttc caaaataata atactaatac taaagttcca ataaatgaaa ttaataaacc
      661 ggcaatatac catacaaacc catgttcagc aaacaatggt aatgataaaa ttccacctgc
      721 tggcaacccc gtcgtatagc atccagcccc accaacaact gcacccccga aaccactcat
      781 tagacatgcc atgaaaaaag cttttttagc cttcaaagta gttccataaa tcagcggttc
      841 tgtaattcct aaaactgagg gaattacatt tgctgctgca accgatttaa aatttttatt
      901 ttttgattta aaagcaatcc ctaatgcagc accagtttgt gcaaatgctg atggcatcgt
      961 cattgctaac aatgcatctc taccagttgt tgcaagatta tttaaattga caggaataaa
     1021 tccacgatgt aaaccaaaaa taactaatgg ctgccaacat gcacaaaata taaatccaaa
     1081 aataattgga ttgaaactat aaactgctaa taatccatct gtaaaaacat ttgttaaaaa
     1141 tgtcactact ggtccaattg ctaataatga taatggagcc ataataatta aatcaatcaa
     1201 tggagtaaca aaacttctaa ttattgaagg cagtctatca cgaatcagct tatccaataa
     1261 tgatgcagca tatactgcaa caataatcgg tactacagaa gatgcatatt taattaaaat
     1321 cacatcaagt cctaagaaag ataactttgc accttcacta aaagctgtca ataaggttgg
     1381 atataacatt gttccagcta aagtgacccc tataaattca tctattttaa agtagcgtga
     1441 tgcactaatc gctaaataga acggcataaa atacaagatt gcatctgcgg cagcatataa
     1501 aatcatatat gttccacttt cagttgttaa tattcccata ctagtaaata tggttaacaa
     1561 tcccctcaat acacctgctc ctgccataac aggaacaaat ggtgtaaata tgcctgaaaa
     1621 taatgcaaat atcttatcca ataatttctt atttttattt gactgttcct ttacagtacc
     1681 tattttagct gttgctaaga ttgctttata tacatcagca acatgggtac caataaccac
     1741 ttgacactgt ccacccgaaa ataacacttg aagaattcct gaattatttt ctaagtttac
     1801 tttatttact ttctcctcat cttttaattc aaatcttaac ctagtagcac aatgctgtac
     1861 tatatttata tttttttcac caccaacatt tttaataata aatcttgcta attcttcata
     1921 tttcattttt tctcctccta ataaaataac ctagaataaa acatgtattc aatcatgttt
     1981 ttattctagg tttagcttgc caaagcaata acaatcctaa tattttaatc ttgcaatatg
     2041 caaagttaaa tacaactttt ccgcctgact taaatctttt ttataaattt tttgaacatg
     2101 tttttctata ttctttacac attcaaatgc ttctggatat ctttcaataa cgatgtcaaa
     2161 taatatatca tctcttttag cttctttttc accaaacatc agccgctttg caaaaaactt
     2221 taaatgtgtc acaaaacgat gatagtctag agaatcctca tcaaatgata tctcacaata
     2281 ttaagtattt cttgaataat tttggtaatt tgagttactt cttccatttc actattaagt
     2341 tcagcattga caaaatgtag agcaataaaa ccaacttcat cttctgataa attaaccatt
     2401 aatttttggt taattatttt caaagcttct gctgcgatct tatattcctc ttgataaaat
     2461 tgcctaatct cccataaaag cggattttga acattaattc cttgtaaata tctctctata
     2521 gcatagttta tatgatcagt aagagttata tatattccat catttaactc tttatccaat
     2581 cctttccgtg caaaatcaat aatttcatca gtaacattaa gaacattgac tggaatatcc
     2641 ttaatcaatt caaggaagcg attgttttgt ttatcatttt ttaaataaaa ttgtttttca
     2701 atcatttcat ctggaacaac ttctccccgt gatttttgat atgcaatacc tcttcccat
//
LOCUS       DAOMTA010000375         2754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1590_length_2754_cov_2.286032, whole genome shotgun sequence.
ACCESSION   DAOMTA010000375 DAOMTA010000000
VERSION     DAOMTA010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_2754_cov_2.286032"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLK68_09990"
     CDS             <1..974
                     /locus_tag="PLK68_09990"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRM90773.1"
                     /translation="NVEQFAYYLVFIGVVIMFLAKKKTIGYIGKVVLGFGLIFVGLQI
                     MSDQMIAFAKEPWFTSIMTTLGKKPWWALLGGTIATGIMQSSTAVIGVVQKLFTTGSI
                     APAAGAAFIFGANIGTCITAVIAALGGSISTKRAAWFHVVYNLAGAIIGMLVLKPFVN
                     LTNWVNLLLNGSQEMYIAQVHFIFNIASTILVIPFVRYCVILLEKLIPGNDHHDTLIE
                     NIDELDDTLIDKLPVGALAVAKKNTLRMGRNVIANIKLSYTYLISKNSEDYDEIIEIE
                     ALIDKYDSRLSKYLLKIAQQPTLAKKQTNEYFKNYQILNELVTLLVI"
     gene            1046..1345
                     /locus_tag="PLK68_09995"
     CDS             1046..1345
                     /locus_tag="PLK68_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRM90774.1"
                     /translation="MYKLALEMVQDALDIYEHDNANKLLSSLNKKEHTLNALEIECRQ
                     NHLVRMRDGICEDNIAASIIIDIISSIERIGDIALNTANSTITVYKDHKVKYLNV"
     gene            complement(1385..1729)
                     /locus_tag="PLK68_10000"
     CDS             complement(1385..1729)
                     /locus_tag="PLK68_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM90775.1"
                     /translation="MIKIEAFVREDKFEDVKEALAKIEVHGITVYQVMGCGIQKGYRE
                     VIRGNEVEINMLPKIKFEIIVSDEVWEKKTIKAIQTAAFTGNPGDGKIISYDLKSALR
                     IRTGETDKDAIN"
     gene            complement(1743..>2754)
                     /locus_tag="PLK68_10005"
     CDS             complement(1743..>2754)
                     /locus_tag="PLK68_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRM90776.1"
                     /translation="LGLSNVGTTPGNYASTIPDLGFAAFQMMFAIITPALITGAVAGR
                     MKFKALFIFIIVWSTIVYYPMAHMVWGLGGFLAEIGSVDFAGGNVVHISSGVSALVLC
                     IILGKRKDYDHAKYRIHNIPFVVLGASLLLFGWFGFNAGSSLKADGLAIHAFMTTAVA
                     AASGMLSWMLMDVWKINKPTLVGSVTGLVVGLVAITPGAGFVPIWSAVIIGFVGSPIC
                     YFMISKAKQYFGYDDALDAFGCHGIGGIWGGIATGLFAQTSINDVARWNGLFFGDTNL
                     LIAQIISILLTIVVAVIGTLICIGVVRLFTPLRVDKRDEQMGLDMSEHNETAYPSFNG
                     LE"
BASE COUNT          928 a          537 c          404 g          885 t
ORIGIN      
        1 tgaatgtgga acaatttgct tactatcttg tctttattgg ggttgtaatc atgtttttag
       61 ctaaaaaaaa gacgattgga tacatcggca aagttgttct gggctttgga ctaatttttg
      121 ttggtctaca aataatgagt gatcaaatga tagcctttgc taaagaacct tggtttacaa
      181 gtatcatgac aacgttagga aaaaaaccat ggtgggcact tctaggtgga actatagcaa
      241 caggcatcat gcaatcatct actgctgtta tcggtgtagt ccaaaaactt tttacaactg
      301 gttcaatcgc tcctgctgct ggtgctgcat ttatttttgg ggccaacatt ggtacttgta
      361 ttactgcggt tattgccgct ttaggcggat ctatttcaac taaacgagct gcctggtttc
      421 atgttgttta taatctagca ggtgcaataa taggaatgct cgttttaaaa ccattcgtta
      481 atttaactaa ttgggtcaat ttgctgctaa acggcagtca agaaatgtat attgctcaag
      541 tacacttcat ttttaatata gcttcaacta ttttagttat tccttttgtt cgctattgtg
      601 ttattttatt agaaaagtta attcctggga atgatcatca tgatacgctc attgaaaata
      661 tcgatgagct agatgatact ttaattgaca aacttcctgt cggagcatta gcagttgcta
      721 aaaagaatac actccgaatg ggacgaaatg ttatagccaa tattaaatta tcttatactt
      781 atttaatctc taaaaatagt gaagattatg atgaaattat cgaaattgaa gcactaatag
      841 ataaatatga ttctcgttta tccaaatact tgctaaaaat tgctcaacag cccacattag
      901 caaaaaagca aactaatgaa tactttaaaa attatcaaat cttgaacgaa ttggtgacat
      961 tgttagtaat ttagctaatt attacaaact tgtttacgat gaaaaaggta aattttctaa
     1021 cgaagcaatc gatgatttaa ataaaatgta taaattagct ttagaaatgg tccaagatgc
     1081 tctagatatt tatgaacacg ataatgcaaa taaacttctt tcttctttaa acaaaaaaga
     1141 acatacttta aatgctttag aaattgagtg tcgtcaaaat catctagtcc gaatgcgtga
     1201 cggtatttgt gaagataata tcgctgcttc tatcatcatt gatattattt ctagtattga
     1261 acgaattggt gatattgctt taaatactgc aaattcaaca attacggtat acaaagatca
     1321 taaagttaaa tatttaaatg tttgattttt acataaaaaa ttactggaat aacatccagt
     1381 aattttaatt tattgcatct ttatcggttt caccggttct aattcgcaaa gcacttttta
     1441 gatcataact aattattttg ccatctccag gattccccgt aaatgctgcg gtttgaattg
     1501 ccttgattgt cttcttttcc caaacttcat cacttactat aatttcaaat ttaatttttg
     1561 gtaacatatt tatttctact tcattacctc taattacttc acgatagccc ttttgaattc
     1621 cacaccccat aacttgataa actgtaatcc catgaacttc aattttagct aaagcttctt
     1681 taacatcctc aaacttatct tcacgaacaa atgcttctat tttaatcaca aatcactcct
     1741 ccttactcta atccattaaa tgatgggtat gctgtctcat tatgttcaga catatctaac
     1801 cccatctgtt catcacgttt atcaactcgt aatggtgtaa atagccgtac aactcctata
     1861 caaattaaag tacctatcac agcaactact attgttagta aaatgctaat aatttgtgca
     1921 attaataagt ttgtatctcc gaagaataac ccattccatc tagctacatc attgattgaa
     1981 gtttgagcaa aaagccccgt tgcaatacca ccccagatac cacctattcc atgacatcca
     2041 aatgcatcta gagcatcatc atatccaaaa tattgttttg ctttagaaat cataaaatag
     2101 caaattgggc taccaacaaa accaataata actgcagacc aaatcggaac aaatcctgca
     2161 ccaggagtaa ttgctacaag cccgacaact aggccggtta cacttcccac taaagttggt
     2221 ttattaattt tccaaacatc catcaacatc catgataaca ttcctgaagc agcagcaaca
     2281 gcagttgtca taaaagcgtg tattgctaat ccgtcagctt ttaaagaact tccggcatta
     2341 aaaccaaacc acccaaataa tagaaggctt gcacctagta caacaaacgg aatattatga
     2401 atacgatatt tcgcatgatc ataatcctta cgtttaccca agataataca caatactagc
     2461 gcgcttactc cagaactaat atgaacaaca tttccaccag caaaatctac tgaaccaatt
     2521 tcagccaaga atccccctaa tccccagacc atgtgagcca taggataata aacgattgtt
     2581 gaccagacaa ttataaaaat aaacaacgct ttaaacttca ttcttcccgc tacagcgcca
     2641 gtaattaaag ctggtgtaat aattgcaaac atcatttgaa atgctgcaaa gcctaaatcc
     2701 ggaatcgtac tggcataatt acctggcgtc gttcctacat tactcaagcc caaa
//
LOCUS       DAOMTA010000376         2747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1598_length_2747_cov_2.198737, whole genome shotgun sequence.
ACCESSION   DAOMTA010000376 DAOMTA010000000
VERSION     DAOMTA010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_2747_cov_2.198737"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLK68_10010"
     CDS             complement(<1..385)
                     /locus_tag="PLK68_10010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRM90777.1"
                     /translation="MKLSIIIPYDRYKQYLHDCLESVYEQRLNDYETLLVVNNKKEID
                     QDIKAYDINLKILEAGENSSVAKKRNIGLEHASGEYIYFIDCDDYLMPNTLKLLITCA
                     NQNDLDLVAGIRRFTWFKKKVFETMG"
     gene            complement(394..780)
                     /gene="tagD"
                     /locus_tag="PLK68_10015"
     CDS             complement(394..780)
                     /gene="tagD"
                     /locus_tag="PLK68_10015"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HRM90778.1"
                     /translation="MKKVITYGTFDLFHVGHLNIIKRAKALGDYLIVAVSSDAFNAQK
                     GKKAYHSDHDRKLILEAIRYVDEVIFEESWDQKIKDVQEHDVDVFVMGDDWEGKFDFL
                     KDYCEVVYLPRTDGISTTKIKDDLHK"
     gene            complement(790..1617)
                     /locus_tag="PLK68_10020"
     CDS             complement(790..1617)
                     /locus_tag="PLK68_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90779.1"
                     /translation="MENYAIKFENVSKIYKLKKKNDGNTKSSETKRFYALKNISFEIP
                     QGEVVGILGTNGSGKSTLSLILAGISEIDEGVMHINGEQSLVAINTGLNKQLTGLENI
                     NVKGALLGLSKKQIQEIIDGVIEFAELGDFLYQPVKKYSSGMKSRLGFSISLYLNPDI
                     IIVDEALSVGDKGFAQKCINKMNELKDEGKTIIFISHSLPQVRNFCQTAMWIEGGMLK
                     EYGEVGEVCDHYGEYVDYYNSLTNKEKAKIRDNKFKKRIVQNPQIGFWEKVLDRIKG"
     gene            complement(1627..2361)
                     /locus_tag="PLK68_10025"
     CDS             complement(1627..2361)
                     /locus_tag="PLK68_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM90780.1"
                     /translation="MLKSIKYVLKENFANPLIQILTYYFVFGLVMNRKDVDGIPFIQW
                     MLAGMVVWFFINPCITQGANAIFSKTGVITKMKFPVSVLPATVVLKELFNHACILLLM
                     IIFYCTQGVYPSIEWLGIIYYCFAACCFAVSLAMITSVLNMIARDTRKLILACMRLIL
                     YLTPILWPISRFSEQTLLFDIIRFVMKINPIYYIVCGYRDCFLYHLGFMHYWKQMLAF
                     WGITGILFVVGCYMMYKFKHKFIDMI"
     gene            complement(2484..>2747)
                     /locus_tag="PLK68_10030"
     CDS             complement(2484..>2747)
                     /locus_tag="PLK68_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRM90781.1"
                     /translation="STGGSSLECVEALKEAGCEVIGMIAIFTYGLPKATINFEAAECK
                     YVTLTDYDTLIGVAKENDYIKEADMEKLKAWKKDPSDESWMSK"
BASE COUNT          947 a          555 c          286 g          959 t
ORIGIN      
        1 cacccatggt ctcaaaaact ttctttttga accatgtaaa gcgtctaatt cctgcaacta
       61 aatctaaatc attttgatta gcacaagtaa ttaataattt taaagtattt ggcatcaaat
      121 aatcatcaca atcaataaaa tagatatatt caccacttgc atgttctaag ccaatatttc
      181 tttttttagc aacactagaa ttttctccag cttcaaggat ttttaaatta atatcatacg
      241 ctttgatatc ttgatcaatc tcttttttat tatttacaac aagtaaagtt tcataatcat
      301 ttaaccgctg ttcataaaca ctttctagac aatcatgtaa atactgtttg taacgatcgt
      361 aagggataat tatactaagt ttcatcttta cacctattta tgtagatcgt ccttaatttt
      421 tgttgttgaa ataccatcag ttcttggtaa atagacaact tcacagtaat cctttaaaaa
      481 atcaaattta ccttcccaat catcacccat tacaaataca tctacatcat gttcttgaac
      541 atctttaatt ttttgatccc aagattcctc aaaaataact tcatcaacgt atctaatcgc
      601 ctctaaaatc aattttcgat catgatcact atgatatgcc tttttcccct tctgcgcatt
      661 aaatgcatca ctacttaccg caacaatcaa gtaatcaccc agcgcttttg ctcttttaat
      721 aatattcaaa tgacctacat gaaataaatc aaatgttccg tatgtaataa ccttcttcat
      781 tattttcacc tatcctttta tccgatctaa aactttttcc caaaatccaa tttgaggatt
      841 ttgaacaatt cgttttttaa atttattatc tcttatttta gccttttctt tgttcgttaa
      901 actgttatag taatcaacat attcaccata atgatcacat acttccccga cttcaccata
      961 ttcttttaac attcctccct caatccacat tgctgtttga caaaaatttc gaacttgagg
     1021 caatgaatgt gaaataaata taatcgtttt accttcatct tttaattcat tcattttatt
     1081 tatgcacttt tgtgcaaaac ctttatcacc taccgataaa gcttcatcaa cgataataat
     1141 atctggattt aaatataaag agattgaaaa accaagtcgt gatttcatcc cgctagaata
     1201 tttttttaca ggttgatata aaaagtctcc tagctccgca aattcaataa caccatcaat
     1261 aatctcctga atctgctttt ttgatagtcc taataaagca cctttaacat taatgttttc
     1321 taaacctgtt agctgtttat ttaaaccggt attaatagca accaatgatt gttcaccatt
     1381 aatatgcatg acaccctcat cgatttcgct aattcccgcc aagataagtg acagagttga
     1441 ttttcctgag ccattagttc ctaaaatccc tactacttcc ccttgtggga tttcaaaact
     1501 aatatttttt aaagcataaa atctttttgt ttcactactt ttagtattac catcattttt
     1561 ctttttcaat ttatatattt ttgaaacatt ttcaaattta attgcgtaat tttccatttc
     1621 taccacctag atcatatcaa taaatttatg tttgaattta tacatcatat aacagccaac
     1681 aacaaacagt attccagtaa tcccccagaa agccagcatt tgtttccaat aatgcataaa
     1741 tccaaggtga tataaaaaac aatctcgata cccacataca atataataga ttggattgat
     1801 tttcatgaca aaacgaataa tatcaaacaa taacgtctgt tcactaaaac gagaaatcgg
     1861 ccataaaata ggtgttaaat ataaaatcaa acgcatacac gctaaaatca acttacgagt
     1921 atctcgagca atcatattaa gcacagatgt aatcatcgct aaactcacag caaaacagca
     1981 ggccgcaaag caataatata taattcccaa ccactcgatt gatggataga caccttgagt
     2041 acaataaaaa ataatcatta aaagcaatat acaggcatga ttaaataatt cctttaaaac
     2101 taccgtagct ggtaaaacac taactggaaa tttcatttta gtaattacac ccgtctttga
     2161 aaaaatggca ttagcccctt gagtaataca aggattaata aaaaaccaaa caaccattcc
     2221 cgccaacatc cattgaataa aaggaattcc atctacatct ttacgattca ttactaaacc
     2281 aaaaacaaaa taatatgtta aaatttgaat caatggatta gcaaaattct cttttagaac
     2341 atatttaata ctttttagca ttttctacct ccaaagctac ttttatttac aacaatagca
     2401 ttataattat agcacaatga taatgcaatt aaaatatctt tattttaggg ggaaaatata
     2461 aaaaaagaag gtgtttttac cttctattta ctcatccagc tttcgtcact tggatctttt
     2521 ttccaagcct ttaatttttc catatcagct tctttaatat agtcattttc ttttgctaca
     2581 ccgatcaatg tatcataatc agtcaatgtt acatatttac attcagccgc ttcaaaatta
     2641 attgttgctt ttggtaatcc atatgtgaaa atagcaatca ttccaattac ttcgcaaccg
     2701 gcttctttta gcgcttcaac gcattctaat gaactccccc ccgttga
//
LOCUS       DAOMTA010000377         2744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1600_length_2744_cov_2.181852, whole genome shotgun sequence.
ACCESSION   DAOMTA010000377 DAOMTA010000000
VERSION     DAOMTA010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_2744_cov_2.181852"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLK68_10035"
     CDS             <1..356
                     /locus_tag="PLK68_10035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM90782.1"
                     /translation="CGNLITFGHNVFINSNAYFMDGAKINIGSNVYIGPSVGLYTAIH
                     PLDYKRRNQGLEKAMPIEIGDNTWLGGNVVVLPGVKIGHGSVIGAGSVVTKDIPPNVL
                     AFGNPCRVVKAIDQS"
     gene            595..1776
                     /locus_tag="PLK68_10040"
     CDS             595..1776
                     /locus_tag="PLK68_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM90783.1"
                     /translation="MKKLQMKYNLLHILYWMATCCIYGYVAVFLQYKGMSNTEIGIVS
                     GSGCILTIFLSPFVTSLISKIKGLTIKQLLSIIYLIIAGAFLTLALVDLSVILIMLLY
                     VSMMSLIVSTVPFLSMIAMNYIQDGKEINFGLARGMGSISYAVSAVLLGQFIEFFNPT
                     ILAYVFVISAILDLVILYSLPNTNVKQATHKKEGNIFTIIKNYKVFFFILLGFSFMFA
                     AATSLSTYLINVVKNLGGNTSLYGVAIFFMAASEMPVMTITPRLIRRYDSITLIMVAA
                     FFYIIRNFTICLAPSLPVLFIGMMMQSLSYGLLTAVITYYVTYNLKSHDQMMGQTMIG
                     IMTSGVGSTIGNLFGGILQDQYGLNMMFIFACLITIIGVIIIFSTGYFQKKQLNTPTK
                     Q"
     gene            complement(1909..>2744)
                     /locus_tag="PLK68_10045"
     CDS             complement(1909..>2744)
                     /locus_tag="PLK68_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM90784.1"
                     /translation="KKVFEDQLKRLDVEYVDFYLLHALDADKWRKVLEYDLIGMCEEF
                     REEGKIRNIGFSFHDEYPVFKEILEYHDWDFCQLQLNYMDMDIQAGMKGYLLAEKYNI
                     PIIVMEPIKGGSLANLPEDIENKFKAYNPDLSISSWALRYVASLPNVKVVLSGMSTYE
                     QVLDNLATFKNFEYLKAEEVALIQDVRDTLKARTQNGCTGCAYCMPCLFGVDIPNNFK
                     YWNNAFVYDSHDQFKAKLEKMVSEAKAENCKQCGACEKMCPQQLPIREDLKRVCEYMK
                     K"
BASE COUNT          886 a          442 c          385 g         1031 t
ORIGIN      
        1 attgtggtaa tttaatcacc tttggtcata atgtatttat taattccaat gcttatttta
       61 tggatggagc caagatcaat attggtagta acgtatatat cggaccttct gttggcctgt
      121 atacagctat tcatccgctt gattataaac gtcgaaacca aggtttagaa aaagcaatgc
      181 caattgaaat tggtgataac acatggcttg gaggtaatgt cgttgtttta ccaggggtaa
      241 aaatagggca tggttcagta attggcgctg gtagtgtcgt taccaaagat ataccaccaa
      301 atgtattagc ttttggtaat ccctgtcgtg ttgttaaagc cattgatcaa tcataattca
      361 cactattaaa atataagtat tactaatttt ataataaaga tattggttct taaaaaatca
      421 atatctttat ttattttttc aatctttgaa aaacctctat ttttcttgct cgaagaaata
      481 aaaattgcta tattaatctt aaaatatgat ggatttacaa ctaaataacg aatagctttg
      541 agtatcatct cgagagctat tttgtttttc atggagagta tgaggacaag aaaaatgaaa
      601 aaactacaaa tgaaatataa tttattgcat attttatatt ggatggcaac ttgttgcatt
      661 tacggatatg ttgccgtatt tcttcaatat aaaggaatga gcaacactga aatcggcatt
      721 gtttctggaa gcgggtgtat tttaacgatt tttttatctc cgtttgtcac atctttaatt
      781 tcaaaaataa aaggcctaac aattaagcag ctattatcta ttatttattt gattattgct
      841 ggagcttttt taactttagc tttagttgat ttatcagtta ttctaatcat gcttttatat
      901 gtttcaatga tgtcattaat tgtttcaaca gttccttttt tatcaatgat tgcaatgaac
      961 tatattcaag acggaaaaga aataaatttt ggcttagcaa gaggaatggg ttctatatct
     1021 tatgctgtta gtgctgtgct cttgggtcag ttcattgaat tttttaaccc gactatttta
     1081 gcatatgtct tcgtcatttc tgcaatttta gatttagtaa ttttatattc tttgccaaat
     1141 accaatgtta aacaggcgac ccataaaaaa gaggggaata tttttacaat tattaaaaat
     1201 tataaagtct tctttttcat tctcttaggc ttctccttta tgtttgctgc tgcaacttcg
     1261 ctatcaactt atttaatcaa tgtcgttaaa aatcttggcg gtaatacttc attgtacggt
     1321 gttgcaatct tcttcatggc agctagcgaa atgcctgtta tgacaattac tccaaggtta
     1381 attagaagat atgacagtat cactttaatt atggttgcag cttttttcta tattattcga
     1441 aattttacta tttgtctagc acctagtctc cctgttttat tcattggaat gatgatgcaa
     1501 agtttatcgt atggattact aacagcagtg ataacttact atgtaacata taacctcaaa
     1561 agtcatgatc aaatgatggg tcaaacaatg atagggatta tgacttctgg ggtaggatct
     1621 actataggaa atctttttgg cggtatttta caagatcaat atggtttaaa tatgatgttt
     1681 atatttgcat gtttaattac aataattgga gtaataatta ttttttcaac tggttacttt
     1741 caaaagaaac aattgaatac acccactaaa caataaaaaa gagattttac tctaattaag
     1801 ttaaaatctc cttttttatt gttcaaccta caaattaaaa taattaacat ctaagtagta
     1861 tttatattta cttttagtct aaataatcag gttcacttaa taccataatt attttttcat
     1921 atattcacat acccgtttta aatcttctcg tatcggcagt tgttgtggac acatcttttc
     1981 acatgctcca cattgtttac aattttcagc tttagcttct gaaaccattt tttctaactt
     2041 tgctttaaat tgatcatgac tatcatatac aaaagcatta ttccaatatt taaaattatt
     2101 tggaatatcc actccaaata gacacggcat gcaatatgca catcctgtac aaccattttg
     2161 agttcttgct tttaatgtat cacgaacatc ttgaatcaaa gcaacttctt cagctttcaa
     2221 atactcaaaa tttttaaaag tagctaaatt atctagtacc tgttcataag ttgacatccc
     2281 gctaagcact actttcacat tgggcaaact ggcaacatag cgaagtgccc atgatgaaat
     2341 acttaaatca ggattatatg ctttaaattt attttcgata tcttctggta aatttgctaa
     2401 cgaaccacct ttgatcggtt ccataactat aattggtata ttatattttt ctgccaataa
     2461 atagcccttc atccctgctt gaatatccat atccatataa tttaattgta attgacaaaa
     2521 atcccaatca tgatattcaa gaatttcttt aaaaaccgga tattcatcat ggaaactaaa
     2581 tccaatatta cggattttcc cttcttctct aaattcttca cacattccaa tcaagtcata
     2641 ttctaatact tttctccatt tatcagcatc taaagcatgt aataaataaa aatcaacata
     2701 ttcaacatct aatcttttta attgatcttc aaaaactttt tttg
//
LOCUS       DAOMTA010000378         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1617_length_2733_cov_2.283420, whole genome shotgun sequence.
ACCESSION   DAOMTA010000378 DAOMTA010000000
VERSION     DAOMTA010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1617_length_2733_cov_2.283420"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1057
                     /locus_tag="PLK68_10050"
     CDS             2..1057
                     /locus_tag="PLK68_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HRM90785.1"
                     /translation="MKNNKVWKDIQKAFNTGVSYMLPSVVVGGILLAVALATGTATDT
                     GMEVTNPFMKNLNDLGSAGFAMMIPILSGYIAYSLAGKPGIAPGMILGYVANNPIGES
                     EVKTGFLGAMILGLATGYLVRWCKTWKVSNTIKTIMPILIVPIFTTFILGIIYIYVIS
                     VPIGACMDWLVKFLSGMQGGNAVLLGAIIGAMTAVDMGGPINKTATAFTLALMAEGVY
                     APNGAHRIACAIPPLALALSTFIDRKKYSADDKNLGISALFMGTIGITEAAIPFAVKD
                     MKRVLPAIIIGSAVGGALGMMNGVEALVPHGGLIILPVVNGKLWYVLAMLVGVLVSAA
                     ILHFTKPNLDIAEKEEK"
     gene            1073..>2733
                     /locus_tag="PLK68_10055"
     CDS             1073..>2733
                     /locus_tag="PLK68_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM90786.1"
                     /translation="MISARMFKIIRFLNQKKESSYKEIGNALDLKERNVRYDIDCIND
                     ALSLKGLSEIEKRSKGQLIVPIDLDLNILLEDSEFIFSADERMQLLRFIIMFDVHSLN
                     IKKLSEKFQVSRRSIQNDLDTLIKEFNQYGLSLVYNRHFHLIEDDHKGYPLRVNELKK
                     YIYLFSNKLSEYNTFELELMKLLYRIYNFDILHIYDWITNKMKMMSWTFSDDSFDWYV
                     ANILTTCWYIIKEKQLPSCPKKIDVEDYSLQELEMIINKQLDDNQKKLIMSYSSYTNK
                     YGEVDINLDLIMTEDIIIQLVTMMSDELGVFFLRDMILIKGLLNHVAPMLERIKNNIR
                     VYELSQTVIPQSYEYVFEVLKSIVLSIQPLQEISQEELIYITIHFIASIQRLRANDYK
                     NILLICGLGFGATALLKDTLRNEYQVQVIDSIPAYDLEHYQKWEQIDIVISTSKLILP
                     VKKTLIVVNAIFDNHDHNKLEAVGIRKKNVLTNYIAIEKRLGFLNSPEREKVLEIIKE
                     ELGYKDVRIPKKYYNISDLLGENCIRYVKSFSNWKDAIKLSTDIL"
BASE COUNT          964 a          303 c          522 g          944 t
ORIGIN      
        1 tatgaaaaat aataaagtat ggaaagatat tcaaaaagcc tttaatactg gggtttcgta
       61 tatgcttcct tcagttgtgg ttggtgggat tctattagcg gttgctttag caacagggac
      121 agcaacggat actggaatgg aagtaactaa tcctttcatg aaaaatttaa atgatttagg
      181 atcagctggt tttgctatga tgattccaat tttatctggg tatatcgctt attcattggc
      241 tggaaaacct gggatagctc cagggatgat cttaggatat gtagcaaata atccaattgg
      301 tgaatcagag gttaaaacag gattcttagg cgcaatgatt ttagggttag caactggtta
      361 tttagttcgt tggtgtaaaa catggaaagt ttcaaatacg attaaaacaa tcatgcctat
      421 tttaattgtt cctattttta caacatttat tttaggaatt atctatattt acgttatttc
      481 tgttccgatt ggagcttgta tggactggct tgttaaattt ttaagtggaa tgcaaggagg
      541 aaatgcagtt ctcttaggtg caatcattgg tgcaatgaca gcagttgata tgggtggacc
      601 aattaataag acggcaacgg catttactct tgcgttgatg gcagaaggtg tttatgctcc
      661 aaatggtgct catcgaattg cttgtgcaat tccgccatta gcattagctt tatctacttt
      721 tattgatcgt aaaaaatata gtgcagatga taagaattta ggaatatcgg cattattcat
      781 gggaacgatt ggaattactg aagcagcgat tccttttgcg gtaaaagata tgaaaagagt
      841 tttaccagca attattattg gtagtgcagt aggtggggca ctagggatga tgaatggagt
      901 cgaagcatta gttcctcatg gtggtttgat tattttacca gtagtcaatg gcaaattatg
      961 gtatgtatta gcgatgctgg taggagtatt agtatccgca gcaatcttac attttacaaa
     1021 gccaaattta gatatagcag aaaaagaaga aaaataaacg gaggaaattg ggatgattag
     1081 tgctagaatg tttaaaatta ttagattttt aaatcaaaaa aaagaatcta gttataaaga
     1141 aatcggcaat gctttagatc ttaaagaacg taatgtacgt tacgacatag attgtattaa
     1201 cgatgctttg agtttaaaag gattgtctga aattgaaaag cgttctaaag gacaattaat
     1261 tgtcccaatt gatttagatt taaatatttt attagaagat agtgaattta ttttttcagc
     1321 tgatgagagg atgcaattat tacgttttat tattatgttt gatgttcata gtctaaatat
     1381 taaaaagtta agtgaaaaat ttcaagtatc gcgtcgttca attcaaaatg atttggatac
     1441 tcttattaaa gaatttaatc agtatggtct atcgctagtc tataatcgtc attttcattt
     1501 aattgaagat gatcataagg gatatccttt aagggtcaat gagttaaaaa aatatatcta
     1561 tttatttagt aataaacttt ctgaatataa tacatttgaa ttagaattaa tgaaattgtt
     1621 atatagaatc tataattttg atattcttca tatttatgac tggatcacta ataaaatgaa
     1681 aatgatgagt tggactttta gtgatgattc gtttgattgg tatgtagcaa atatattaac
     1741 tacttgctgg tatattataa aagagaagca attgccatct tgtcctaaaa aaattgatgt
     1801 agaagattat tcactccaag aactggaaat gataattaat aaacaattag atgataatca
     1861 aaaaaaacta attatgagtt attctagtta tactaataaa tatggtgagg ttgatatcaa
     1921 tttggattta ataatgactg aagatattat aattcaactt gtgacaatga tgtctgatga
     1981 gttaggagta ttttttttac gggatatgat tttaataaaa ggattattga atcatgtggc
     2041 accgatgttg gaacgaataa aaaataatat tcgagtttat gagttaagtc aaacagtgat
     2101 tccacaaagt tatgaatatg tatttgaagt tttaaaatca attgtattga gtattcaacc
     2161 gttacaagag atcagtcaag aagaattaat ttatattact attcatttta ttgcaagtat
     2221 tcaaagatta agagcaaatg actataaaaa tattttacta atatgtggct taggttttgg
     2281 cgctacagca cttttaaaag atactctaag aaatgaatat caggttcaag tgattgatag
     2341 tattccagcg tatgatctag agcattatca gaaatgggag caaatagata ttgttatttc
     2401 aacgtcaaag ttaatcctcc cagtaaaaaa aactttgatt gttgtaaatg caatttttga
     2461 taatcatgat cataataaat tagaagcggt gggaattaga aaaaagaatg ttttaacgaa
     2521 ttatattgca attgaaaaaa gattgggatt tttaaattct cctgagcgtg aaaaagtact
     2581 tgaaataatt aaagaagaac ttggctataa agatgttagg attcctaaga aatactataa
     2641 tataagtgat ttattaggtg aaaattgtat tcgttatgtt aaatcattta gcaattggaa
     2701 agatgcaatc aaactttcaa ctgatatttt aat
//
LOCUS       DAOMTA010000379         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1618_length_2733_cov_2.102315, whole genome shotgun sequence.
ACCESSION   DAOMTA010000379 DAOMTA010000000
VERSION     DAOMTA010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_2733_cov_2.102315"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..421)
                     /locus_tag="PLK68_10060"
     CDS             complement(53..421)
                     /locus_tag="PLK68_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HRM90787.1"
                     /translation="MALLVDGDACPDLPAIKDLAWKYQVEMTVFVDYAHFIQDDYFRT
                     ILCEVGSDSVDLVLLKQVQANDLVITQDYGLASLVLSKGAKVLHISGKVIAKNTHHIN
                     LNPGIYIVKSGFKTMKIIIH"
     gene            complement(409..990)
                     /locus_tag="PLK68_10065"
     CDS             complement(409..990)
                     /locus_tag="PLK68_10065"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRM90788.1"
                     /translation="MITNAIIIISIIIFILINFIDKSNYDKTSLAIKYGAFYMPRIEV
                     KKEYWRFITANFVHIDIMHIFMNVYCIYNLGHFFEYLLGTGAYLYLIFISCLATSGAC
                     YYHGKKYAYAYNTVTLGASGIFYGYLGAMIALGILVGGYFDYMLQQYIYVILINVAFT
                     FFNRQVSKAGHFGGLAGGFLATAMLIGCGVWHF"
     gene            complement(991..1791)
                     /locus_tag="PLK68_10070"
     CDS             complement(991..1791)
                     /locus_tag="PLK68_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM90789.1"
                     /translation="MKNRIISVVLSFFVIVNIILIACAFNVTPLTIKRDTFVYEYGSE
                     ISTKPQDYINANEAILSQVILNFSNLKNEIGEYKVSATYLGVEYPFYIKIVDTTKPVV
                     TLKASTFNVHLNTEVYAIDLIEKVEDNSDIAAYFIDENGEKSTHKVFTEKGSYVERII
                     VEDQAGNQSASLRVKIVAGQNGNNPTLTGIDDIEVLKNSKFNPLDGVKATDGSGNDIT
                     KNIKILKNNVNTDKVGDYEVIYSITNDKGHNLQRTRRVSVIKTEKAGE"
     gene            complement(1969..>2733)
                     /locus_tag="PLK68_10075"
     CDS             complement(1969..>2733)
                     /locus_tag="PLK68_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM90790.1"
                     /translation="KELEDLFEEALHNHEFEVYFQPKYSTKKKRLYGAEALVRWKSSK
                     LGSISPGEFIPVLERSSNIIELDEYVFVITCKQIRKWLDDGLEVAPIAVNVSQLHLYR
                     QDFVESYLKVIDAYQIPYNLIELEMTETSLFDNRDILKDILNKFRKLKITVSMDDFGS
                     GYSSIMMLESMPIDCLKIDKTMIDDLEVNSKAKEILKSIISLAQSLGLVVVAEGVEQA
                     GQYEELIRMKVDYIQGYYCARPLPLDEYEKILKLKK"
BASE COUNT          971 a          522 c          292 g          948 t
ORIGIN      
        1 ccaaattaat aaccagtgat aaaaaaataa aaaataaaac tgtttttttc atttaatgta
       61 taattatttt cattgtttta aagcctgatt ttacaatata aattccggga tttaaattta
      121 tgtgatgagt atttttggca attacttttc ctgaaatatg caaaacttta gccccttttg
      181 ataacactaa acttgctaga ccatagtcct gagtaataac aagatcattt gcttgaacct
      241 gtttcaataa aactagatca acactgtcac ttcccacttc acatagaata gttctaaaat
      301 aatcatcctg tataaaatga gcataatcaa caaaaaccgt catttcaacc tgatatttcc
      361 aagctaaatc tttaattgct ggtaaatcag gacaagcatc accatcaact aaaagtgcca
      421 tacaccacaa ccaatcaaca ttgcagttgc taaaaagcca cctgccagac ctccaaaatg
      481 tcccgccttt gatacctgac gattgaaaaa tgtaaaagct acattaatta aaatcacata
      541 tatatattgt tgcaacatat aatcaaaata accgcctaca agtattccta aagcaatcat
      601 tgcacctaaa taaccataaa aaatcccact agctcctaaa gtcacagtat tatatgcata
      661 agcatatttt tttccatgat aataacaagc tccgctagta gcaagacagc ttatgaagat
      721 tagatataaa tatgcgcccg ttcctaataa gtattcaaaa aaatggccaa gattataaat
      781 acaatataca ttcataaaaa tatgcataat atcgatatga acgaaattag cggtaataaa
      841 acgccaatat tcttttttta cttcaattcg cggcatataa aaagccccgt atttaatcgc
      901 taaagaggtt ttatcataat tactcttatc aataaaatta attaaaataa agattataat
      961 cgatatgata ataatagcat tggttatcat ctattctcct gccttttctg ttttaatgac
     1021 actaacgcgt cttgttcttt ggagattatg tcccttatca tttgtaattg aataaattac
     1081 ttcataatca ccaactttat ctgtattaac attatttttt aaaattttta tattcttagt
     1141 aatatcattc ccactaccat cagtagcttt cactccatca agaggattaa atttactatt
     1201 ttttaatacc tcaatatcat caattcctgt aagagtagga ttattaccat tttgtccagc
     1261 tactatttta actcgtaaac tggcagattg atttcctgcc tgatcttcaa caattatcct
     1321 ttcaacataa gaaccttttt cagtgaacac tttatgtgtt gacttctcac cattttcatc
     1381 aataaaatat gcagctatat ctgaattatc ctcaaccttt tcaattaaat caattgcata
     1441 tacttcagta tttaaatgaa cattaaacgt agatgctttt aatgttacaa ctggtttagt
     1501 tgtatcaact atctttatat aaaaaggata ttcaacacct aaatatgtag cagatacttt
     1561 atattcacca atttcatttt ttaaattcga aaaattcaaa ataacttgtg ataaaattgc
     1621 ctcattagca ttaatataat cttgcggctt agtcgatatt tcactaccat attcataaac
     1681 aaatgtatct cttttgatcg ttaggggggt aacattgaag gcacaagcaa ttaaaattat
     1741 atttactatg acaaagaagc ttaaaacaac tgatataatt cgatttttca tgctatcacc
     1801 ctctcccatc tttaaatcca tttttctata ttcacgaact atcttatgct ttttccagct
     1861 aaaaatcaat gagattttat gattattaag actttttaag tttcaataat cataacttag
     1921 aaatttctaa aaaaattcaa aaaattacgt taaaataatt gaatcttttt acttttttaa
     1981 ttttaaaatt ttctcatact catctaatgg cagcggtcga gcacaataat atccctgaat
     2041 ataatctacc ttcattctaa ttaattcctc atactgtcca gcctgttcta caccttcagc
     2101 tacaacgact agccctaaag attgagctaa actaataatc gatttcaaaa tttctttagc
     2161 ctttgaattg acctccaaat catcaatcat tgttttatca atttttaaac aatcgattgg
     2221 cattgattcg agcatcatga ttgaagaata acctgaacca aaatcatcca tagatactgt
     2281 aatctttaat ttcctaaatt tattgagaat atcttttaaa atatcacgat tatcaaacaa
     2341 acttgtttcc gtcatctcta attcaattaa attatagggt atctgataag catcaattac
     2401 ttttagataa ctttcaacaa aatcttgacg atataaatgt aattgcgaaa catttaccgc
     2461 tataggagca acctcaagac catcatcaag ccatttacga atctgcttac acgtaattac
     2521 aaaaacatac tcatccaatt caataatatt actgctgcgt tctaaaaccg gtataaattc
     2581 tccaggactt atcgatccta gtttactgct tttccaccta accagcgcct cagctccata
     2641 aagtcttttt ttctttgtac tatattttgg ctgaaaataa acttcaaatt catgattatg
     2701 caaggcttcc tcaaacaagt cttccaactc ttt
//
LOCUS       DAOMTA010000380         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1622_length_2729_cov_2.447644, whole genome shotgun sequence.
ACCESSION   DAOMTA010000380 DAOMTA010000000
VERSION     DAOMTA010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_2729_cov_2.447644"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLK68_10080"
     CDS             complement(<1..267)
                     /locus_tag="PLK68_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90791.1"
                     /translation="MNSIREEFAVTSLAMDLTKDLIATGCYSENKIHFWNVSNQTKVK
                     TIRFDKAFNPINSILFFKNNIAFIANSAANTVILHGENFSEIQKL"
     gene            460..666
                     /locus_tag="PLK68_10085"
     CDS             460..666
                     /locus_tag="PLK68_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90792.1"
                     /translation="MNNEEKARLQEIFKSSKGIVDHEFSQDIIDACQADEDARRQRVL
                     NGETTLEQEEEALMAEWEKLHTHH"
     gene            747..1070
                     /locus_tag="PLK68_10090"
     CDS             747..1070
                     /locus_tag="PLK68_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90793.1"
                     /translation="MDLRIGFSKAKQCDLTNYKGEKLLVHSVVIFDEELSYEDEILVV
                     HQWESTFTPQWKDRDIAEATKKAKAIIKSILENGDSDEIGYRLEPSTVIIGDQYENDC
                     KVYQV"
     gene            1236..2219
                     /locus_tag="PLK68_10095"
     CDS             1236..2219
                     /locus_tag="PLK68_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRM90794.1"
                     /translation="MSEYSDQERQEFYAKVKETLNEPVTSREQADRHTQLEYKITTTR
                     SDLLHKNPIKGDFDFNHLAQIHHKLFDGIQDFAGKQRHFDIYKAIPSPEGKKEVGYFL
                     KAKDIHFSFEDFTKEIKDSNSLKGLNKEQFVDKFTNLYANINEIHPFEEGNGRATKLM
                     MKQLANEAGYQVNFDQVEKREWNYACKRALSDQTQFRGSDQFSMSKDIQLLKDVMNKI
                     VHSLQIKQDNVIDEKETLKKTALDLAPNAQVHKASPGRYEGRIVAETENFVAQRLGDF
                     SKHFVVHEKKSFDQPPKVNEVVSITHKANTDTAKVENMQNKELSKSKEIKR"
     gene            complement(2255..>2729)
                     /locus_tag="PLK68_10100"
     CDS             complement(2255..>2729)
                     /locus_tag="PLK68_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM90795.1"
                     /translation="EESNQGDILLVESVDRLSRLSQDDFSILKEKIKSKGLKLIIHDL
                     PTTHINNATSDKMTSEILNVINSMLIDLLATMARLDNEKRKERIKQGLERAGYKPTGK
                     KPNLKKHERIKVLLSQGAMTKEEIALAVGVGEATVYRVQKQLNPSSNMKNNIESQ"
BASE COUNT          907 a          421 c          536 g          865 t
ORIGIN      
        1 taacttttgt atttcgctga aattttcacc gtgtaggatc acagtatttg cagctgaatt
       61 ggcaatgaaa gcaatattgt tcttaaagaa aagtattgaa ttaatagggt taaatgcttt
      121 atcaaaacgg attgttttga cttttgtttg gttgcttaca ttccaaaaat ggatcttgtt
      181 ttcggaatag caaccagtgg ctattaaatc tttggtcaag tccatcgcca gggacgtgac
      241 tgcaaattct tctctaatgc tgttcatatt aaactactgc tttgaagttg cgctaattat
      301 gccttattta tggatatttc cataattcca gtgtgttggt gagtatatta aatgagctaa
      361 aatacttaca ataacacttt acaattacat acaaattttc ggatacgaaa tctaaaaaaa
      421 tcaagtataa tattcaaatg actaagagga tttatatcta tgaacaatga agaaaaagca
      481 agattgcaag agatctttaa aagttctaaa ggtattgttg atcatgagtt ttctcaagat
      541 attatagacg cctgtcaagc ggatgaagat gctagaaggc aacgtgtatt gaatggtgaa
      601 acgactttgg aacaagaaga agaagctttg atggctgagt gggaaaagtt acatactcat
      661 cattaatttt tgggtttaaa gaaatcagca gttgtcagtt ccacaaataa acgagagttt
      721 acgtaaatag attggagtaa aacgttatgg atttaagaat tggattttcc aaggcaaaac
      781 aatgtgactt gaccaactac aaaggcgaga aactacttgt tcattcagtc gtgatttttg
      841 atgaagaatt aagttatgaa gatgaaatcc ttgttgttca tcaatgggaa agcaccttta
      901 cacctcaatg gaaagatcgt gatatagcag aagcaacaaa gaaagctaaa gctattatta
      961 agtcaatttt agaaaatggc gatagtgatg aaattggtta tcgcttagag ccaagcactg
     1021 taatcattgg cgatcagtat gaaaatgact gcaaggttta tcaagtttaa tcctgcttat
     1081 tagtggaaaa ctgcaagcta ctctttctta tttcggttac tactctaatt tattactgtg
     1141 aaattttagt agtaagacat tgatttttta tatagccact ctaattaatt tgaggaactg
     1201 acagcagtag tgctgatttt ttttaggagt gaaaagtgag cgagtattca gatcaagaac
     1261 gacaagaatt ttatgcaaaa gtaaaagaaa cattaaatga acctgttacg tcaagagagc
     1321 aagcagatag gcatactcaa ttagagtata aaattactac aacacgctca gatttgctgc
     1381 acaaaaatcc tataaagggg gattttgatt ttaatcattt ggcgcaaata catcataagc
     1441 tatttgatgg aattcaggat tttgcaggaa agcaaagaca ctttgatatt tataaggcta
     1501 taccctcacc agaggggaaa aaggaagtcg gttacttcct aaaggccaaa gatattcatt
     1561 tctcatttga agactttaca aaggaaatta aagatagcaa ttccttaaag gggctgaata
     1621 aagaacaatt cgttgataaa tttacaaact tgtatgccaa cataaatgag attcaccctt
     1681 ttgaggaggg gaacggtcgg gcaactaaat tgatgatgaa gcagctggcg aatgaagctg
     1741 ggtatcaggt caattttgat caggtggaaa agcgtgagtg gaactatgcg tgtaaacgag
     1801 ctttaagtga tcaaacacaa tttcgtggct ctgatcagtt ttccatgtca aaggatattc
     1861 agttgctaaa agacgtaatg aataagatag tccattcatt acaaattaaa caagacaacg
     1921 ttattgatga gaaagaaaca ttaaaaaaaa cagcgcttga tttagcgcct aacgcccagg
     1981 tacacaaagc aagtcctgga agatatgagg gccgtatagt tgctgaaaca gaaaattttg
     2041 ttgcccaaag attaggggat tttagtaaac actttgtagt ccatgaaaag aaaagctttg
     2101 atcaaccacc taaagttaat gaagtggttt caattacgca taaagccaat actgatactg
     2161 caaaggtgga aaatatgcag aataaagagc tatctaagtc taaggaaatt aaacgttaat
     2221 tttggtgcta tacatgaaaa gcatgattat cactttattg gctctctatg ttatttttca
     2281 tattactgct tgggtttaat tgtttttgta ctcgatacac tgttgcttct cctacgccta
     2341 cagctaatgc aatttcttct ttggtcatag ctccttgaga cagtagcact ttaatccgtt
     2401 catgtttttt gagattaggc ttttttcctg ttggtttgta gccagctcgt tctaggccct
     2461 gcttaattct ctctttcctt ttttcattat ctagtcgggc cattgtcgcc aataagtcga
     2521 taagcattga gttgataaca tttaatattt cgcttgtcat cttatcgctg gtcgcattgt
     2581 tgatatgtgt ggttggtaaa tcatgaatga ttagtttgag acctttagat ttaatctttt
     2641 ccttgagtat ggaaaaatcg tcctggctta atcttgaaag ccgatctaca gattcaacaa
     2701 gtagaatatc cccctgattg gattcttct
//
LOCUS       DAOMTA010000381         2725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1625_length_2725_cov_2.276404, whole genome shotgun sequence.
ACCESSION   DAOMTA010000381 DAOMTA010000000
VERSION     DAOMTA010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1625_length_2725_cov_2.276404"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1590)
                     /locus_tag="PLK68_10105"
     CDS             complement(220..1590)
                     /locus_tag="PLK68_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRM90796.1"
                     /translation="MGNYNFDDLQYLQIPLPEELLKLKWHGSFTRMKRIIDAKLANDI
                     PIALKKRLLHEKEIIRRIPQEYPLSYEEALKICHNNFNDFSDEELEKLQDENAVEWIF
                     VEGQPYFRADFFDNILKTRNDYAARLFDKSKVESREANFALLNNAIHEIKTKGQLTYH
                     FRLKTGIKIKASDSNKKIKIHLPLPLEDCQVKNFKLIATMPQYKYLNDGSHPQRTVYF
                     EENIDQVKDFYVEYEYDNEMKYVELKPEDVLESQPNFYLHEQAPHIVFTPYLKSLAKE
                     IVKDETNNLIKAKLIYEYITTHITYSFVRNYYTIPNIPEYAALGGKGDCGVQALLFIT
                     LCRCVGIPARWQAGLYVTPQDIGNHDWARFYIAPYGWLYADCSFGGSAYRNGDQERWQ
                     YYFGHLDPFRMPANSEYQYDLYPPKQFIRQDPYDNQTGEAEFEDRGLYLDEYDLHLEV
                     LEIKKV"
     gene            complement(1606..>2064)
                     /locus_tag="PLK68_10110"
     CDS             complement(1606..>2064)
                     /locus_tag="PLK68_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90797.1"
                     /translation="IINQKWGLNKEIVAYQYGYLKKYVRIINNPIVEIKEIGREYFEF
                     IKENYSTPIEEAYLLERIDANVFVGAFIEKQIVGFAGRHIEGTIGFVEVIKEYRRLGI
                     AQALEQYLMQKLIAENEIIYLQVEVDNYPSMRLHEKLGYERSDDIITWYM"
     assembly_gap    2065..2090
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2091..2307)
                     /locus_tag="PLK68_10115"
     CDS             complement(<2091..2307)
                     /locus_tag="PLK68_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90798.1"
                     /translation="MKNEILKFLKQDYLNNLDLIYALEHGAKIKYYNQAGIMLKFEDI
                     YMLAFKDEKIAEQLLKQIDKCSMLAIHN"
     gene            complement(2347..>2725)
                     /locus_tag="PLK68_10120"
     CDS             complement(2347..>2725)
                     /locus_tag="PLK68_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM90799.1"
                     /translation="GSIFIVGLAIIALFMIANTIKITITARQTEISIMRMVGASNWYI
                     RIPFMLEGMLIGLIGSIIPIIVLVYGYGMVYDYANGALMSAMLALKPPMPFIRDFSLV
                     IAAVGAGVGLVGSFVSIRRFLKF"
BASE COUNT          898 a          508 c          342 g          951 t
ORIGIN      
        1 caagataaga taccggtgat gcttcacgat agagaacttc atcatcctcg caccagcacc
       61 ccaacaaatc ataaatattt gtcatttctt tttcattata tttactaaaa ccttgattga
      121 aagttaaaaa gttagtcaca ccatataaat cgatcattcc ctcaacatct tggggatatt
      181 ctggaaaagt ttgataacta taatttttaa cttttataat taaactttct tgatctctag
      241 tacttctaaa tgtaaatcgt attcatctaa atacagccca cgatcttcaa actcagcctc
      301 accagtttga ttatcatatg gatcttgacg aataaattgt ttcggtggat aaagatcgta
      361 ttgatattca ctatttgcag gcattctaaa cggatcaagg tgtccaaaat aatactgcca
      421 tcgttcctga tcaccattac gataagcaga acctccaaaa gaacaatcag catataacca
      481 gccatatgga gcaatataaa atcttgccca atcatgatta ccgatatcct gtggagttac
      541 atataaacct gcttgccaac gggcaggaat ccccacgcaa cgacataaag taataaataa
      601 tagtgcttga acaccacaat caccttttcc acctaaagca gcatactcag gaatattggg
      661 aattgtgtaa taattccgaa caaatgaata ggttatgtga gttgtaatat actcatatat
      721 taatttagct ttaattaaat tatttgtctc gtctttaact atttcttttg ccaaagactt
      781 taaataaggg gtaaaaacaa tatgcggtgc ttgttcatgt aaataaaaat taggttgact
      841 ttctaaaaca tcttcgggtt taagttctac atatttcatc tcattatcat attcatactc
      901 cacataaaaa tctttaacct gatcaatatt ttcttcaaaa tagacagttc gctgtggatg
      961 acttccgtca tttaaatatt tgtattgcgg cattgtcgca attaatttaa aattcttaac
     1021 ttgacaatct tctaacggca aaggaagatg aattttaatt tttttatttg aatctgatgc
     1081 cttgatctta attcccgtct ttaaacgaaa atgatatgtt aattgtccct ttgttttaat
     1141 ttcatgtatt gcattattaa gcagtgcaaa atttgcttca cgactttcaa ctttgctttt
     1201 atcaaacaaa cgtgcagcat aatcatttct agtctttaaa atgttatcaa aaaaatcagc
     1261 tctaaagtat ggttgtcctt ctacaaaaat ccattctacc gcattttcat cttgtaattt
     1321 ttctagttcc tcatcactaa aatcattaaa attattatga caaattttta aagcttcttc
     1381 atatgataac ggatattctt gaggtattct acgaataatt tctttttcat gaagcagtcg
     1441 tttttttaaa gctatcggaa tatcattagc taattttgca tcaattattc ttttcattct
     1501 tgtaaaacta ccatgccatt ttaattttaa taattcctct ggcaacggaa tttgtaaata
     1561 ctgtaagtca tcaaaattat aatttcccat tattctttcc tttctttaca tataccaggt
     1621 gataatatca tcagaacgct catagcctaa tttttcatgt agtcgcattg atggataatt
     1681 atctacttct acttgtaaat aaataatttc gttttcagca attagttttt gcattaaata
     1741 ctgttctaga gcctgagcta ttccaagacg tcgatattct ttaattactt caacaaaacc
     1801 aattgttccc tctatatgac gcccagcaaa tccaacaatc tgcttttcaa taaacgcacc
     1861 cacaaataca ttagcatcaa ttcgttcaag caaatatgct tcttcaatcg gagtagaata
     1921 attttcttta ataaattcaa aatattccct cccaatttct ttaatttcaa cgattgggtt
     1981 attaattatt ctaacatatt tttttaaata gccatactgg taagctacaa tttctttatt
     2041 taatccccat ttttgattaa ttatnnnnnn nnnnnnnnnn nnnnnnnnnn cattgtgaat
     2101 tgctagcatt gagcacttat ctatttgttt aagtaattgt tcagcaattt tttcatcttt
     2161 gaatgcaagc atatagatat cctcaaattt aagcattatt cctgcctgat tataatattt
     2221 tatcttagca ccatgttcaa gagcatatat taaatctaaa ttattaagat aatcttgttt
     2281 aagaaatttt aatatctcat ttttcatcat tcctactcct attaaaaaaa caggcactgc
     2341 ctgtttttag aattttaaga atcgtctaat tgaaacaaag cttccaacca aaccaacgcc
     2401 agcccctaca gcagcaataa ctagtgagaa atcgcggata aatggcattg gtggttttag
     2461 tgctaacatt gctgacatta atgcaccatt agcgtaatca tataccattc cataaccata
     2521 aactaaaaca ataatcggga taattgatcc aattagacca atcaacattc cttcaagcat
     2581 aaaaggaata cggatatacc agttactagc accgaccata cgcataatac tgatttcagt
     2641 ttgacgcgct gtgatcgtga ttttaattgt attagcgatc ataaataatg caataatggc
     2701 aagtccgaca ataaaaatcg atcct
//
LOCUS       DAOMTA010000382         2716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1640_length_2716_cov_2.125517, whole genome shotgun sequence.
ACCESSION   DAOMTA010000382 DAOMTA010000000
VERSION     DAOMTA010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1640_length_2716_cov_2.125517"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLK68_10125"
     CDS             <1..273
                     /locus_tag="PLK68_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM90800.1"
                     /translation="IESEVGKGTHVTGEFIKNHLDTPPMGNIVETIITLIQADEKINY
                     LFKYTSDKLEFILDTQEIKKILEGVPINQPEIIVWLKEYIKEGLQL"
     assembly_gap    381..408
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <409..664
                     /locus_tag="PLK68_10130"
     CDS             <409..664
                     /locus_tag="PLK68_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRM90801.1"
                     /translation="IAAGARPVLNSLVETVAQEKLPATVLQTGCIGMCTLEPIVEVFD
                     RDDNKTTYVLVDSAKAKEIAEKHLKHDEIIDEYTVGHYKK"
     gene            676..2475
                     /locus_tag="PLK68_10135"
     CDS             676..2475
                     /locus_tag="PLK68_10135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRM90802.1"
                     /translation="MPTKRTQVLVCAGTGCTIGNSGELITEFEKEIKALGLEKEVEVL
                     RTGCLGLCGVGPNISIYPDNIIYKTVQVSDVKEIVMEHFYKGRPVHRLMLNESDEETK
                     EIHDINDTKFYNKQKRIALHNCGVIDPENINEYIGKDGYFALAKVVSEMTPQEVVDVI
                     KASGLRGRGGGGFPAGVKWQFALNEPGKEKYVICNADEGDPGAFMDRSILEGNPHSVI
                     EAMAIAAYAIGANHGYIYIRAEYPIAVERLNTALEQARELGLIGKNIFESGFDFDLEL
                     RLGAGAFVCGEETALIQSIEGERGMPNPKPPFPAHKGVWGKPTIINNVETYANIAQII
                     QHGAEWFRSIGTETSPGTKVFALGGKIVNTGLVEVPMGTTLREVIYEIGGGCPNHKRF
                     KAVQTGGPSGGCLTEEQLDTPIGFDELVKLGSMMGSGGMIVLDEDNCMVDVARFYMDF
                     IVDESCGKCTPCRVGTKRMLELLEQICDGKGTMETLDELELLASTIQDTALCGLGQTA
                     PNPVLSTIHQFRDEYIAHIVDKKCPAGVCKELLQYEIDEDKCRKCGLCAKQCPVGAIS
                     GELGKVPYVIDQEKCIKCGQCIKACHFNVIERK"
     gene            2485..>2716
                     /locus_tag="PLK68_10140"
     CDS             2485..>2716
                     /locus_tag="PLK68_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRM90803.1"
                     /translation="MSKIKMTINNREVEAYEGQTVLEAAKNNGIHIPTLCYLKDVTGT
                     GACRVCQVEIEGAKTLCAACVYPVREGLVVKTN"
BASE COUNT          930 a          403 c          570 g          785 t
ORIGIN      
        1 atcgaatccg aagttggaaa aggtactcat gtaactggtg aatttattaa aaatcatttg
       61 gatacgccac caatgggaaa tattgttgaa acaattatta ctttaattca agctgatgaa
      121 aaaattaatt atttatttaa atatacaagt gataaattag aatttatttt agacactcaa
      181 gaaattaaaa aaattcttga gggtgttcca attaatcaac cagagataat tgtctggtta
      241 aaagaatata taaaggaggg attgcaacta tgaaatcctt agaagattta aaaaaacttc
      301 gtgatgctgc tcaaagcaac atgtcaatgc gaacagatga acaacaaaaa tatcgggttg
      361 tcgtaggaat ggcaacttgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ttgcagcagg
      421 agcgcgtcct gttttaaact ctttagtaga aactgttgcc caagaaaaat tacctgcaac
      481 agtattacaa actggatgta ttggaatgtg cactctagaa ccaatcgtag aagtgtttga
      541 tcgcgatgac aataaaacaa catatgtatt agtagactct gctaaagcta aagaaattgc
      601 tgaaaaacac ttaaaacatg atgaaataat cgacgagtat actgtcggac attataagaa
      661 ataggaggtt gtaagatgcc aacaaaaaga acccaagtgt tagtttgtgc tggaactggt
      721 tgtacaatcg gtaattcagg tgagctaatc acggaattcg aaaaagaaat caaagctctt
      781 gggcttgaaa aagaagttga agttttaaga actggttgtt taggattatg tggagttggt
      841 ccaaatattt ctatctatcc agataatatc atttataaaa ctgttcaggt ttctgatgtt
      901 aaagaaatcg ttatggaaca cttctataaa ggtcgtccag ttcatcgttt aatgttaaat
      961 gaatctgatg aagaaacaaa agagattcat gatataaatg atactaaatt ctataataaa
     1021 caaaaaagaa tcgcccttca taattgtggt gtaattgacc cagaaaacat taatgaatat
     1081 atcgggaaag atggctactt tgccttagct aaagtagtta gcgaaatgac tccacaagaa
     1141 gtcgtagatg taattaaagc tagtggatta cgcggccgtg gaggtggtgg attccctgct
     1201 ggagttaaat ggcaatttgc tttaaatgaa cctggtaaag aaaaatatgt tatttgtaac
     1261 gctgatgaag gtgatcctgg agcatttatg gatcgttcaa ttcttgaggg aaatccccac
     1321 agtgttattg aagcaatggc tattgccgct tatgcaattg gtgctaacca tggatatatt
     1381 tatattcgag ctgaatatcc tattgccgta gaacgtttaa atacagcttt agagcaagca
     1441 cgtgagctag gactaatcgg taaaaatatt tttgagagtg gttttgattt tgatttagaa
     1501 ctacgtttag gagctggagc cttcgtttgt ggtgaagaaa cagctttgat tcaatcaatt
     1561 gaaggtgaac ggggaatgcc taatccgaaa ccaccatttc ctgcccataa aggggtctgg
     1621 ggaaaaccaa ctattattaa caacgttgaa acatatgcca atattgctca aattattcaa
     1681 catggtgctg aatggtttag gagcattggt actgaaacat cacctggtac aaaagtattt
     1741 gcccttggag gaaaaattgt taatactgga ttagttgaag ttccaatggg aactacttta
     1801 cgtgaagtaa tttatgaaat tggtggtggc tgcccaaatc acaaacgctt taaagcagtt
     1861 caaacaggtg gtccatcagg tggctgtttg actgaagaac aactagatac accaattggt
     1921 tttgatgaat tagtaaaact tggctcaatg atgggatcag gtggaatgat cgttctcgac
     1981 gaagacaact gtatggtcga tgtagcccgc ttctatatgg attttattgt tgatgaatca
     2041 tgtggtaaat gcactccttg ccgtgtagga accaaacgaa tgctagaatt gcttgaacaa
     2101 atttgtgacg gtaaaggaac aatggaaact ttagatgaac ttgaattact agcttcaaca
     2161 attcaagata ctgctctatg cggcttaggt caaactgctc ctaaccctgt actttctacg
     2221 atccatcaat ttagagatga atatattgca catatcgttg ataaaaaatg tcctgctggg
     2281 gtatgtaaag aattattaca atatgaaatc gatgaagata aatgtcgtaa gtgtggtctt
     2341 tgtgctaaac aatgtccagt tggagcaatc agtggtgaac ttggaaaagt tccatatgtt
     2401 atcgatcaag aaaaatgtat taaatgtggt caatgtatta aagcatgcca ctttaatgta
     2461 attgaaagaa agtaggtgta aggaatgtcg aaaataaaaa tgactatcaa caaccgtgag
     2521 gtagaggcat atgaaggcca aacagtcttg gaagcagcca aaaataacgg aatccatatc
     2581 cctactttat gttatttaaa agatgttaca ggaacaggtg cttgccgtgt ttgccaagtt
     2641 gaaatcgaag gtgcaaaaac attatgcgct gcctgtgttt atcctgttag agaaggacta
     2701 gtagttaaaa ctaatt
//
LOCUS       DAOMTA010000383         2711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1644_length_2711_cov_2.163780, whole genome shotgun sequence.
ACCESSION   DAOMTA010000383 DAOMTA010000000
VERSION     DAOMTA010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_2711_cov_2.163780"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PLK68_10145"
     CDS             <1..914
                     /locus_tag="PLK68_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRM90804.1"
                     /translation="SFGDTPASWSDSMNIETAKSIYGTVLTDEIVDDKVVRPDLSNVD
                     KVIVGMRSPNNGSLFNQSGMIVEDGKQKFYPLSLQYRPYTADGENVRKVSIAGDLNSD
                     GTQQNRSYYGATSKIANEYDLTACLEAVEAAKAVNKDIPVVVALNANGPVIVSEFEDK
                     VDAIVCGFSVSDSAVFDIINGKSEPKGLLPLQFPANMDTVEANKEDVAGDLVCYKDTQ
                     GNTYDVAYGLNWSGIIDDERVQTYNPDRPTTDTTTVTPTPTPDKPTTSVKTGDEINLN
                     TTMLAGAVALVAGLTAYVTAKKRKAVK"
     gene            977..2329
                     /locus_tag="PLK68_10150"
     CDS             977..2329
                     /locus_tag="PLK68_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRM90805.1"
                     /translation="MKILKKLMILLVLAALIIGCSKEPENNYLGNLKVGYTMKLDDKE
                     VPKAKFEFFYGRALTKYLNQYYSAFSDDYADINDLKNQDCTVTGYTDKSWEYYFADQA
                     KKDILEVEALVRGAKTAGYQINADDKKKARSTFGELESYCKENKIDFEDYLHSTFGLE
                     ATKDNLISYYEEANLASRYAAVLLKEPTDEEVEQYYLANKNDIDTINIRYFAFAKDDK
                     AKADEFAQKIRSEADFKQMAVDYSSDDKKIYYQNNDLSLRQDLRKSDLPEYLQSVLFD
                     QALPINSVKVVEGDSSIDVVMLVAREQPVYRQASISTVYLDARETDTDNLTTEKMNTC
                     KEFADNLLQDFMNNTDRSIDKFHEYNSKYADDKNNQGDYDNISKGDSTIEIANWVFDE
                     SRQVGDLEVLMSNYGYTIVYFRGFGGIDYFERAKVLAKEATYDKQLNDLKRNIKVMIK
                     "
     gene            2406..>2711
                     /locus_tag="PLK68_10155"
     CDS             2406..>2711
                     /locus_tag="PLK68_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM90806.1"
                     /translation="MKRRRVVVTVLIIVIIGLMMVYSASNIWAGYKFNDSLYYIKRQG
                     IFAVIGIIAMFVFSKIDYHIYQKNANKLLIGSFILMILVLIPGIGAVRGGSRSWFNLG
                     "
BASE COUNT          974 a          323 c          545 g          869 t
ORIGIN      
        1 ggagttttgg tgatacacct gcatcttggt cagattcaat gaatattgag acagctaaat
       61 ctatctatgg aacagtttta actgatgaaa tagttgatga taaagtagta cgacctgatt
      121 tatcaaatgt tgataaagta attgttggaa tgcgttctcc aaataacgga agcttattta
      181 atcaatcagg tatgatcgtt gaagatggta aacaaaaatt ttatccatta tcattgcaat
      241 accgcccata tactgctgat ggcgaaaacg taagaaaagt gtcaattgct ggagatttaa
      301 atagtgatgg tacccaacaa aatagatctt attatggagc aactagtaaa attgctaatg
      361 aatacgattt aacagcttgt ttggaagctg tagaagcagc taaagcagtc aataaagata
      421 ttcctgtagt tgttgcttta aatgctaatg gtcctgttat cgttagtgaa tttgaagaca
      481 aagtagatgc aatcgtatgt ggcttctctg taagtgatag tgctgtgttt gatattatta
      541 atggtaaatc tgaaccaaaa ggattattac cattacaatt tcctgcaaat atggatactg
      601 tagaagcgaa taaagaagat gttgctggtg atctagtatg ttataaagat acacaaggaa
      661 atacttatga tgtagcctat ggtttaaact ggtcaggaat tattgatgat gagcgtgttc
      721 aaacatataa tccagaccgt ccaactacag atacaacaac agttacccct acaccaacac
      781 ctgataaacc tactacttca gtaaaaactg gtgatgaaat taatttaaac acaacaatgc
      841 tggctggggc cgttgctcta gtggcaggat taactgcata tgtaacagct aaaaaaagaa
      901 aagcagtaaa ataaaatgat aaagatgata tagaggtatg gttcagcata cctctataat
      961 taattgaggt gagcatatga aaatacttaa aaaattaatg attttgctag tactggcagc
     1021 tttgataata ggttgttcca aagaaccaga aaataattat ttaggaaatc tgaaagttgg
     1081 ctatacaatg aaactagatg ataaagaggt gccaaaagca aaatttgagt ttttctatgg
     1141 tcgcgctttg actaaatatt taaaccaata ttacagtgct tttagtgatg attatgcaga
     1201 tataaatgat ttaaaaaatc aagattgtac ggttactggt tatacagaca aatcatggga
     1261 atattatttt gctgatcagg caaagaaaga tattttagaa gtagaggcat tggtaagagg
     1321 tgctaaaact gctggttatc aaataaatgc tgatgataaa aagaaagctc gctcaacatt
     1381 tggtgaacta gaaagttatt gtaaagaaaa taagattgat tttgaagatt atctccattc
     1441 aacctttggt ttagaggcaa caaaggataa tttaatcagt tattatgagg aagctaatct
     1501 ggcctcacgc tatgcagcag ttttattaaa agaaccaact gatgaagagg tggagcaata
     1561 ttatctagct aataaaaatg atattgatac gatcaatatt cgctattttg cttttgctaa
     1621 ggatgataaa gcaaaagcag atgagtttgc ccaaaagatt cgtagtgagg cagattttaa
     1681 acagatggca gttgattata gtagtgatga taaaaaaatt tattatcaaa ataatgattt
     1741 gagtctacgt caagatttaa gaaaatcaga tttacctgaa tatttgcaat ccgtattatt
     1801 tgatcaggca ttaccaatta atagtgttaa agtagttgaa ggggattcta gtattgatgt
     1861 agttatgctg gtggctagag aacagcctgt ttatcgacaa gctagtattt caacagttta
     1921 tttagatgct agagaaacag atactgataa tttgacaacc gaaaaaatga atacatgtaa
     1981 agaatttgca gacaatttgc tacaagattt tatgaataat actgacagat cgattgataa
     2041 gtttcatgaa tataatagta aatatgcaga tgataagaac aatcagggag attatgacaa
     2101 tatatctaaa ggggatagta ctattgaaat tgcaaattgg gtttttgatg aatctcgaca
     2161 agttggagat ttggaagtat taatgagtaa ttatggttat accattgtat attttagagg
     2221 atttggagga attgattatt ttgaaagagc aaaggtatta gctaaagaag caacgtatga
     2281 taagcagtta aatgatttaa aaaggaatat caaggttatg attaaataaa aagtttatca
     2341 ggaatgataa gctttttttc atttcttaaa atattttttc atatacttaa aatggaggta
     2401 agtatatgaa acgtagaaga gtggtcgtta cagttttaat aattgtgata attgggttga
     2461 tgatggtata tagtgcctcg aatatttggg ccggatataa atttaatgat tctttatatt
     2521 acattaaacg acaaggaatt tttgctgtta ttggtataat tgctatgttt gtgttttcaa
     2581 agattgatta tcatatttat caaaaaaatg caaataaact tttgattggg tcttttatct
     2641 tgatgatttt agttttgatt ccggggattg gagcagttcg cggggggtcg cgctcatggt
     2701 ttaatttagg t
//
LOCUS       DAOMTA010000384         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1651_length_2701_cov_2.243764, whole genome shotgun sequence.
ACCESSION   DAOMTA010000384 DAOMTA010000000
VERSION     DAOMTA010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1651_length_2701_cov_2.243764"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLK68_10160"
     CDS             complement(<1..278)
                     /locus_tag="PLK68_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRM90807.1"
                     /translation="MSKIKNNALGVILCLAIAMPAYYLGKQLPLIGGPVFAILMGMII
                     TFLVKDKSKVQSGITYTSKKILQYAVILLGFGMNLSDIVKTGSSSLPI"
     gene            388..1263
                     /locus_tag="PLK68_10165"
     CDS             388..1263
                     /locus_tag="PLK68_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM90808.1"
                     /translation="MLDFRIDTFLAVCKYMNFTKASQYLNITQPAVSGHIRYLEEYYD
                     VKLFMYIGKKMQLTKAGKILLDVATTLKHDDIFLKNKLNNLSLKQELVFGATLTVGEY
                     VMPKVIDQLLNENQNTMIKMQVANTSELLKMINEGQLDFALVEGYFNKLEYDYRKFCQ
                     DEYICVGSIDFPLSIVHDISELFKYNLIIRENGSGSREIIERWLKERNLDIDDFSNII
                     EIGNINMIKRLVKNNHGITFIYKLAERRLKQVKVNALTIKHDINFIWRKNSVFNDYYE
                     ELFEVFRLQNDKVLL"
     gene            1288..1419
                     /locus_tag="PLK68_10170"
     CDS             1288..1419
                     /locus_tag="PLK68_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90809.1"
                     /translation="MPLTDDDFLKDSKLELYNEVIESLQEQEETTDVLDYEFDLEEE"
     gene            1457..2380
                     /locus_tag="PLK68_10175"
     CDS             1457..2380
                     /locus_tag="PLK68_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90810.1"
                     /translation="MIEEYIANNQFQDALDLLNDMDDEMTRYQRLVCLYGLGEYQMAK
                     AEGMLAKAKASNTYYDVVSIYVATLKELEEFEEAINIMVEELSMPYIPYEYEAIFNAA
                     HDELLLAKREANEGMERHNNAFSLDDMENILMKDLPNEDLLYMAIEQMEGINIRRLLP
                     AIRNFLKDENKPSFAKSLLIELMIDQEIDEEMTLVKKGIEYGINPSYAPLVLNQEVGG
                     TILNLLSEGIEDDNPSLYSLCEQFLNFYLYLVYPKYIDDYDYRPIAAAIHYHIASMQY
                     IDIELDDIEYLYNCDKEEILEKLNEIKEIEY"
     gene            2474..>2701
                     /locus_tag="PLK68_10180"
     CDS             2474..>2701
                     /locus_tag="PLK68_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRM90811.1"
                     /translation="MKINNKKLENAIVELTVAFDSEEWKATQEKALDKLAKNVKIDGF
                     RPGKAPAAMVRARVSKASVLEEATDMILQTKF"
BASE COUNT         1007 a          290 c          494 g          910 t
ORIGIN      
        1 attattggta aagatgaact tcctgttttt acaatatcgc ttaaattcat accaaatcct
       61 aataatataa cggcgtattg taagattttt ttagaagtat atgtgatccc actttgaact
      121 ttgcttttat ctttaactaa gaatgtaatg atcattccca ttaaaatggc aaatacggga
      181 ccgccaatca atggcagttg tttaccgaga tagtatgctg gcattgcaat agctagacat
      241 agaataactc ctaaagcatt attttttatt tttgacattt ctaatccccc tttgtcgaag
      301 ttattataca taaatcaaac aataaataaa aattatatat atttatataa cgataataat
      361 atgttatagt tataatgagg tgatttaatg ttagattttc ggatcgatac ttttttggcg
      421 gtatgcaaat atatgaattt tactaaagca agtcagtatt taaatattac tcagccagct
      481 gtttcgggtc atattcgtta tttagaggaa tattatgatg ttaagttatt tatgtatatt
      541 ggtaaaaaga tgcaattaac taaagcagga aaaattttgc ttgatgtagc tacaacatta
      601 aagcatgatg atattttttt gaaaaataaa ttaaataatt tgtcattgaa acaagagctg
      661 gtttttggag caacacttac agtaggggaa tatgtgatgc ctaaagtgat tgatcaattg
      721 ttaaatgaaa atcaaaacac aatgattaag atgcaggttg caaatacaag tgagttatta
      781 aagatgatca atgaaggaca acttgatttt gctttagttg aaggatactt taataaatta
      841 gagtatgatt atcgaaaatt ttgtcaagat gaatatatat gtgttggttc aattgatttt
      901 ccattgtcaa tagtccatga tattagtgaa ttatttaagt ataatctaat tattcgtgaa
      961 aatggctcag gttcacgaga aattattgaa cgatggttaa aagagcggaa tttagatatt
     1021 gatgatttta gtaatattat tgaaattggt aatattaata tgatcaaacg tcttgttaaa
     1081 aataatcatg gaataacttt tatctataaa ttagctgaaa gaagattgaa gcaagtcaaa
     1141 gtaaatgctt taacaataaa acatgatatt aattttatct ggcgtaaaaa tagtgttttt
     1201 aatgactact acgaagaatt atttgaggtg tttcgattgc aaaatgataa agtattgcta
     1261 taattacgtt tgtaaggagt gatgattatg cctttgacag atgatgattt tttaaaggat
     1321 agtaagttag aactttataa tgaagtgatt gaaagtctcc aagaacaaga agaaactaca
     1381 gatgtcttgg attatgaatt tgatttagaa gaagaatagt gatgtatcct cgatacatca
     1441 tttttaagga gaaaatatga ttgaagaata tatagctaat aatcaatttc aagatgcact
     1501 tgatctattg aatgatatgg atgacgaaat gacgcggtat cagcgtcttg tttgtcttta
     1561 tggtttaggt gaatatcaaa tggctaaagc tgaaggaatg ttagcaaaag cgaaagcaag
     1621 taatacctat tatgatgttg taagtattta tgtggctact ttaaaggaac tagaggagtt
     1681 tgaagaagca atcaatatca tggtagaaga attatcaatg ccatatattc cgtatgaata
     1741 tgaagcaatc tttaatgctg ctcatgatga actgctgtta gctaaacgtg aagcaaacga
     1801 gggaatggaa cggcataata atgcttttag tttggatgat atggaaaata ttttgatgaa
     1861 agatttgcct aatgaggatt tattgtatat ggcaattgaa caaatggaag gaattaatat
     1921 tcggcgccta ttaccagcta ttcgtaattt tttgaaagat gaaaataagc ctagcttcgc
     1981 taagagttta ctcattgaat tgatgattga tcaagaaata gatgaagaaa tgaccttggt
     2041 aaaaaagggg attgaatatg gaattaatcc aagttatgct ccattagttt tgaatcaaga
     2101 agttggtgga acgatcttaa atcttttatc tgaaggaatt gaagatgata atccttcgtt
     2161 atattcattg tgtgagcagt ttctaaattt ctatctatat ttagtttatc ctaaatatat
     2221 cgatgattat gattatcgtc caattgctgc agcaattcat tatcatattg cgtcgatgca
     2281 gtatattgac attgaattag atgatatcga gtacctttat aattgtgata aagaagaaat
     2341 tttagaaaaa ttaaatgaaa ttaaagaaat tgaatattaa agtactgaaa ttgcaagatt
     2401 aattgttcaa aaaagttgaa ttaatagtat tgttagtata taatacgtga tgaaactaaa
     2461 aaaggagaat agtatgaaaa tcaataataa aaaattagaa aatgcaatcg tagaactaac
     2521 agtagcattt gatagtgaag aatggaaagc tactcaagaa aaagcacttg ataaattagc
     2581 taaaaatgta aaaatcgatg gttttagacc aggtaaagca cctgcagcta tggtaagagc
     2641 aagagtttct aaagctagtg ttttagaaga agcaacagat atgattttac aaacaaaatt
     2701 t
//
LOCUS       DAOMTA010000385         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1658_length_2696_cov_1.878076, whole genome shotgun sequence.
ACCESSION   DAOMTA010000385 DAOMTA010000000
VERSION     DAOMTA010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_2696_cov_1.878076"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="PLK68_10185"
     CDS             <1..1578
                     /locus_tag="PLK68_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRM90812.1"
                     /translation="ITPPWYFLACYVLYVLILLIGALQKGAILKIPMIIISVVAMAIT
                     YISFPISTYRPSKYSLFDNARTPISTPGNIKEEYNVNSQGDRHYRNSLDFTIAGEVTL
                     NNFKIRGIAYDLYEDGKWGTSHSRVETEWFKNNLEKIANITKTSRQVIEVNQISGYSQ
                     RNYTPYFIINDDMTYYGDHYEGKNPQTYEMIIPNDDFNALLSTIDYKAKGELLKEIAE
                     RNGTQDYYDEYFGQGNEDDEKLTSVPEETKSIIENFLKQHNVIDNGNIFNYITQCTNA
                     LAANTSYTLRPGNTPDNVDVVDYFLNTNKKGYCVHYASTLALMLRSRGYPARFVVGYQ
                     VPGSKNNAGKLIVRDSNAHAWVEIYDEYLGWIPIEATPTSSENPNTPTDTITPAPNQG
                     DKTPQPVEPEKPNIQQISQNDSFQIPVYIYYLAGGMIFIFIVLFQARIRKKRMFKGAA
                     NSNQKVCYYYYYLTKLKINCDVIKTIIDKARFSQHQISSEELEIVEQFYQDKTNAYFK
                     QANLFKKIYLRYLIAVL"
     gene            1638..1841
                     /locus_tag="PLK68_10190"
     CDS             1638..1841
                     /locus_tag="PLK68_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90813.1"
                     /translation="MAKLIKETTKSERENYLNKLFACKNGDCENCGVCKIFAGTSPQE
                     VYYDYIIGKREFLEISQTFNSRK"
BASE COUNT          968 a          337 c          474 g          917 t
ORIGIN      
        1 attacaccac catggtattt tctagcatgt tatgttttat atgttttgat tttattgatt
       61 ggtgctttgc aaaaaggggc tatattaaaa ataccaatga ttatcattag tgtagttgcc
      121 atggcaataa catatatatc atttccaata agtacatatc gtccttcaaa gtatagtttg
      181 tttgataatg ccagaacacc aatttcaact cctggaaata taaaagagga atacaatgtc
      241 aatagccaag gggatcgtca ttatcgtaac tcattagatt ttacaattgc tggtgaagtg
      301 actttaaata atttcaagat tcgaggaatt gcttatgact tgtatgaaga tggtaaatgg
      361 gggacttctc atagtcgcgt tgaaacagaa tggtttaaaa ataacctaga gaagattgca
      421 aatattacaa agacttctcg tcaagttatt gaagttaatc aaatcagtgg ttatagtcaa
      481 cgtaattata caccatactt tatcattaat gacgatatga catattatgg tgaccactat
      541 gagggtaaaa accctcaaac atatgagatg attataccta atgatgattt taatgctcta
      601 ttatcaacga tcgattataa agccaaagga gaattattaa aggaaatagc cgagcgtaat
      661 ggaacacagg actactacga tgaatatttt ggacaaggga atgaagatga tgaaaaatta
      721 acatcagttc ctgaggaaac aaaatcgatt attgagaatt ttttaaagca gcataatgtt
      781 attgataatg gtaatatttt caattatatt acacagtgta caaatgcttt agcggctaat
      841 acttcttata ccttgagacc gggaaataca cctgataatg tggatgttgt tgattatttt
      901 ttaaatacta ataagaaagg gtattgtgtt cactatgctt caacattagc attaatgtta
      961 agaagtaggg gatatcccgc acgttttgtt gttggctatc aagtgccggg aagtaagaac
     1021 aatgctggta aattgatagt tagggatagt aatgctcatg cttgggttga aatttatgat
     1081 gaatatttgg gctggatacc aattgaagcg actccaacaa gtagtgaaaa tccgaataca
     1141 ccaactgata caattactcc tgcacctaac caaggtgata aaacaccaca gccagtggag
     1201 ccagaaaaac cgaatattca acaaatttct caaaatgata gttttcaaat tcctgtttat
     1261 atttattatt tagcaggagg gatgatcttt atctttatag ttttatttca agcaagaatt
     1321 cgtaaaaaac gaatgtttaa aggagctgct aatagtaatc aaaaagtctg ttattattat
     1381 tactatttaa ctaaattgaa aattaattgt gatgtgataa agacaataat tgataaagct
     1441 cgtttttcac aacatcaaat atctagtgaa gaacttgaaa tagtagagca gttttatcaa
     1501 gataaaacaa atgcatattt taaacaagca aatttgttca aaaaaatcta tttacgttat
     1561 ttaatagccg ttttatagag caaagcacgc gaattaattg cgtgcttttg ctataattga
     1621 attaaggaga taggaaaatg gctaaattga ttaaagaaac tactaaaagt gaacgagaaa
     1681 actatcttaa caagcttttt gcatgtaaaa atggggactg tgaaaattgt ggagtttgta
     1741 agatttttgc aggtacttca ccgcaggaag tttattatga ttatataata ggaaaacgtg
     1801 aatttttgga gattagtcaa acttttaata gtcgcaaata aaatacagtg cttattaact
     1861 gattgtaagt tgctaaaaca ctgtattttt atatgcaatc caaataagtt attaaagctt
     1921 gaagttttct agtttcgtta tttctaacgg aataagggaa taatattcat gatctgatcc
     1981 atattctgaa gacttttttg ttctaataat atattttttc taattgtaat agcactgccc
     2041 gttagagctt tttgtaatta taggttaatt gctatttggt agaatattta ctgaatagtg
     2101 atgacagata tcgaaggcat tataatagta taggtgtaaa tctatactga caaaagtctt
     2161 agatagtaaa tgtgattgaa ataattaagc tgatctttaa actgtttaag tcgatttttt
     2221 atcatatttg atacatagac tttaataaat tatattttaa tcaactattt cattaaattt
     2281 ttatcttatt cttgatttat taaaaatact tttaaaagtc tatttaaaat attctcttga
     2341 gatgttttta ggaaagaaat ttaagtttaa ttcttttatt atctttttaa agccagtctt
     2401 cttttaggaa ataaaaaagg tttacatcaa ttcaaatgat taatgaatta gagaaactat
     2461 atcagattaa aactaataat tagtctgctt ttgcattcaa tcgtattgac aaagagaaat
     2521 atgtgtgata gattttattc atagataaac ttgtgattgg gagataaaac atcgaacgcg
     2581 cttgtagaga gtctagggtg gtggaagcta gatagagatg cgactttggt aaatggaccc
     2641 atgaaggcaa aggggaaaga agtaatttga gtatcttttg acgttagctt acgtta
//
LOCUS       DAOMTA010000386         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1664_length_2692_cov_2.290861, whole genome shotgun sequence.
ACCESSION   DAOMTA010000386 DAOMTA010000000
VERSION     DAOMTA010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1664_length_2692_cov_2.290861"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1218..>2692)
                     /locus_tag="PLK68_10195"
     CDS             complement(1218..>2692)
                     /locus_tag="PLK68_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRM90814.1"
                     /translation="SKDFVYDNINKCYWTKDNNGKLISETKNNDNSYTITSSGVKPDG
                     PKYNYFNYMKYKYNKLYTCGGRVIAGNFFNRKGTVQVLDNDNWNVFEDEIDDITKVPY
                     LDVSSISIDPNDTTHIFAASARTALYEFYKGKYKNHYSIENSNDAIHSAIINNGIENP
                     EYVLVTGSTYDNEGNLWLLTSQTKNTLIEYSKDKIWSTHNKNEILTSNGMLQNMIIDK
                     NSNLWFSNNHWVSPALFYYDIKNDAVKAYKTFVNQSGATLELNGGIHCIAEDKEGNIW
                     IGSNVGPLILSNTAIKNNNSIFTQVIIPRNDGTSYADYLLSGIDISCIAIDGANRKWI
                     GTFDKGLYLISADNMTQIHHFISSNSSLLSDAIESLAINDNTGEVFIGTDKGLCSYMS
                     DATVTYDNMTKDNVYAYPNPVDPNYTGLISIVGLSYNSNVKIITASGTIITEGISNGG
                     MFTWDGKDKHGNRVASGIYFAVTAKSDGSSGTVCKIAMIK"
BASE COUNT          930 a          376 c          331 g         1055 t
ORIGIN      
        1 tgttcagata aaaagtttta tcctgtacct cctgaaatat ctgaaggtgg attatgatgt
       61 agttgtaatg attttgaata tatgaaaatg aatactttgg agaaaattta taatactctt
      121 aagtatgaat ggccttcagt agaagtacca gataatatta gaaaagaagc tttaaaacct
      181 atagaaagga tgttggaact gagttaattt cagtttcaac tttatataag tatagtaata
      241 tctatatttt atattatcta tttaccaata gtccatggga taaatttata tataatccat
      301 gttattgatg tacatattat gtatacaata attaatgcta ttattatcct ataatattgg
      361 ccattatagt aaaaatttat tttattgagt atagtacttg ttaataatgg tacaccacca
      421 cataaaaaat aatatgttat gctatgagaa cctgtatatt ttataaattt gtaattgggt
      481 atttttttgc ttatattaat aagtaaataa ataccaacta tactgtctat taagaaaata
      541 atataattac tgattgtcaa tggacatatc attatattta tcttataata atattctatg
      601 tatttaataa atataataat aataattgat aatgatacgt ataatgtatt gttataattg
      661 ttgataactt tttcattttt atgatacaaa tatcctaagt acaagtatat catagacata
      721 taagatatat tataattcca aatttttaaa ttgttagtgt tgataaatgc tgatattata
      781 aataaaagta tgcatactat aggcataata aatctattat atttttcttt tatcaacaat
      841 aaaatagaaa atataaactc agaaatagcc aatgatgtta taaaccatga tgcttttcct
      901 gttaatatat tatttattat tgcatatatg ttgttgtttt catatataag agctttgcaa
      961 acagctataa aaatagaaaa tataaagtaa ggtataatta tgcttcttaa tatatatatt
     1021 agcttatttt gtaaacaaaa aggtttatta ttataaaaaa gatatccgga tataaaataa
     1081 aaagctatta aagcattact tacatataga ttgtatttta ttatttcgcc accggagtaa
     1141 taaatttctg tatgaaataa tagaattgct atcatacatt ccgcggataa tatcaatcca
     1201 tatgattctt ttcattatta ttttatcata gcaattttac atacagtacc ggaacttcca
     1261 tctgattttg ctgtaacagc aaagtaaatg cctgatgcta ccctattccc atgcttatct
     1321 ttgccatccc atgtaaacat gcctccatta cttatacctt ctgtaataat tgtaccacta
     1381 gcagttatta tttttacatt tgaattataa gataatccaa ctattgatat aagtccggta
     1441 taatttggat ctacaggatt tggataagca tatacattat ctttggtcat attatcatat
     1501 gtaaccgtag catcactcat atatgaacaa agtcctttat cagtgcctat aaagacttct
     1561 cctgtattat cattaatagc taatgattct atagcatcag acaataaact gctgttagac
     1621 gaaataaaat gatgtatttg tgtcatattg tctgcactta ttagatacaa tcctttgtcg
     1681 aaagtaccta tccattttcg gttggcacca tcaatagcaa tgcatgaaat atctattcca
     1741 cttaatagat aatctgcata agatgtacca tcattacgag ggataataac ttgtgtgaat
     1801 atactattat tattttttat tgcagtattt gatagtatta atggtcctac gtttgatcct
     1861 atccatatat taccttcttt atcttctgct atgcagtgta tacctccatt aagttctaat
     1921 gtagctccac tctgattaac gaaagtctta tatgctttta ctgcatcatt tttgatatca
     1981 taataaaata atgcaggact tacccaatga ttattactaa accataaatt actattttta
     2041 tcaataatca tattctgaag catcccatta ctggttaata tttcattttt gttgtgagta
     2101 ctccaaattt tatctttaga atattctatt aatgtatttt ttgtttgact ggtcaacagc
     2161 cataaattgc cttcattatc gtatgtactg cctgtaacta aaacgtattc tggattttct
     2221 ataccattat ttattatagc agagtgtatt gcatcattgc tgttttctat tgaataatga
     2281 tttttatatt ttcccttata aaattcgtat aaggccgtac gtgcagaagc tgcaaatata
     2341 tgagttgtat cattgggatc tattgaaata cttgatacat caaggtatgg aacctttgtt
     2401 atgtcatcta tttcatcttc aaatacattc caattatcat tatccagtac ttgtacagta
     2461 cctttacggt taaaaaaatt tccggctata actcttcctc cacaagtata taatttatta
     2521 tatttatact tcatataatt gaaataatta tattttggtc catcaggttt tacaccagac
     2581 gatgtgatag tataactatt atcattattt ttagtttctg atatgagttt tccattatta
     2641 tctttagtcc aatagcattt gtttatattg tcataaacaa aatctttact ac
//
LOCUS       DAOMTA010000387         2690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1666_length_2690_cov_2.648197, whole genome shotgun sequence.
ACCESSION   DAOMTA010000387 DAOMTA010000000
VERSION     DAOMTA010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_2690_cov_2.648197"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PLK68_10200"
     CDS             <1..1709
                     /locus_tag="PLK68_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM90815.1"
                     /translation="NEIYPFLEDYNIANGKGGKWYDTFNGTDASMKTSAVDNIITSRN
                     LEISNITMVETDLSDHNLFYADAKLLDQPVASRDYLDLVISDANALDEGKYSNASYNT
                     LDEKLVAGELLDQNATQEQINKAVKDIQTAQDNLKTRYPNLALNKDVSVSGLEVNDGR
                     WTAPMAVDGIVSSDSRVSLAKDKDEQWLEVDLGKTETISNFVINYESQVPAFKIQVAG
                     EDKVYRDIYEVSNLEGQISNIQKISIDPTEARYVKYVQLKRWTHSGNGKQYSGSIYEF
                     EVYEQDPDAKDYSNIALNRPVSASSLEVGDGRFTAPMAVDGVVSKDSRVSFGKTADNQ
                     WLLVDLGSRKTVSEFVINYESCPPAFKIQVSTDGVNYQDVYEAADLPDGGPSEIKEIQ
                     IEATKARYVKYVQLKRWTHTNKNKYSGSIYEFEVYKERDHTVTTADEVLKSLNKEAPQ
                     INGNKIVLPAVPDGFEISLYGCDNKQVVAMDGTITRPLEDMDINVLYQVKNLNDETDI
                     AYSEADINFIVPGAYNKEESINAKPNVMPGLREWKGNEGEFTLLLKMPVWKLLLNKFS
                     FI"
     gene            1673..>2690
                     /locus_tag="PLK68_10205"
     CDS             1673..>2690
                     /locus_tag="PLK68_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HRM90816.1"
                     /translation="MEATAKQIQFYLKEMIKHELEIVTSGEKSGDIVLVKDGSVDNLG
                     KEGYTLAIDDMIEIKSNYETGILYGGISITQMFSQNDGMNNVAKGLARDYPKYEVRAG
                     MLDVARTYIPMEYLQEMTIYMAYYKLNEVQVHVNDYWGATGYSAFRLECDTYPMITAT
                     DGSYTKEEYRQYQIDMKNYGINVITEIDTPYHAECFRNVPGVKMLKAGALDIREQSSY
                     DVIEAVLDEYLDGPNPVIQSDKFHIGTDEYDKSYSEQMRKWTDHFINYVNDKGYDTRL
                     WGSLGSRGFNGTTPVSNDATVNLWAPYWADVKETYNAGYDIINTCGGWLYIVPGANAG
                     YPDRL"
BASE COUNT          943 a          343 c          575 g          829 t
ORIGIN      
        1 acaatgaaat ttatccattt ttagaagatt acaacattgc aaatggtaaa ggtggaaagt
       61 ggtacgatac ctttaatggt actgatgcaa gtatgaagac tagtgcagtc gataatatta
      121 ttacttctag aaatttagaa attagtaata ttacaatggt tgaaacagat ttatctgatc
      181 ataatttatt ttatgccgat gctaagttat tagatcaacc agttgcttct agagattatt
      241 tagatcttgt gattagcgat gctaatgctt tagatgaagg taaatatagt aacgcttctt
      301 acaacacttt agatgaaaaa ttggttgcag gggaactgct tgatcaaaat gctactcaag
      361 agcaaatcaa taaggctgtt aaagatattc aaacagcaca ggataatctt aaaacaagat
      421 atcctaattt agctctaaat aaagacgtta gtgtatcagg actagaggtg aatgatggaa
      481 gatggactgc tccaatggca gtagatggta ttgtttctag tgatagtcgt gtatctttgg
      541 ctaaagataa agatgagcaa tggttagaag ttgacctagg taaaacagaa actatttcta
      601 attttgtgat taattatgaa tcacaagtac ctgctttcaa gattcaagtt gctggtgaag
      661 ataaagtgta ccgtgatatt tatgaggtta gtaatttaga ggggcaaatt agcaatattc
      721 aaaagatttc aattgatcct actgaagcac gatatgttaa atatgtccaa ttaaaacgct
      781 ggacacatag tggtaatggg aaacaataca gtggcagtat ttatgaattt gaggtatatg
      841 aacaagatcc agatgccaaa gattacagta atattgcatt gaatcggcct gttagcgctt
      901 catcattaga agtaggtgat ggccgcttta ctgcaccaat ggcagtagat ggagtggttt
      961 ctaaggacag tcgggtatct tttggtaaaa ctgcagataa tcaatggtta ttagtagatt
     1021 taggaagtcg taagacagta tctgaatttg taatcaatta tgaatcatgc ccaccagcat
     1081 ttaaaattca agtatctact gacggtgtaa actatcaaga tgtttatgaa gctgctgatc
     1141 ttccagatgg aggacctagt gaaattaaag agattcaaat tgaagcaaca aaagcaagat
     1201 atgttaagta tgttcaatta aaacgctgga cccatactaa taaaaataaa tatagtggca
     1261 gtatttatga atttgaagta tataaagaaa gagatcatac agttacaact gctgatgaag
     1321 ttttaaagtc attgaataag gaagcgccac aaatcaatgg taataaaatt gttttaccag
     1381 cggtacctga tggctttgaa attagtttat acggttgcga taataaacag gttgtagcaa
     1441 tggatggaac aattactcgg ccattggaag atatggatat taatgtatta tatcaagtta
     1501 aaaacttgaa tgatgaaact gatattgctt atagtgaagc cgatattaac tttattgtac
     1561 ctggagctta taacaaggaa gaaagtatta atgctaagcc taatgtaatg ccgggattac
     1621 gggaatggaa aggtaatgaa ggagaattca cattattgtt aaagatgcca gtttggaagc
     1681 tactgctaaa caaattcagt tttatttaaa agagatgatc aaacatgaac ttgaaattgt
     1741 tactagtggt gaaaagagtg gtgatattgt tttagtaaaa gatggtagtg ttgacaactt
     1801 agggaaagaa ggatatacgc ttgccatcga tgatatgatt gaaattaaat ctaactatga
     1861 aacaggaatt ttatatggtg gtatttcaat tacccaaatg ttttcgcaga atgatgggat
     1921 gaataatgta gctaaaggat tagcgcggga ctaccctaaa tatgaggttc gggcaggtat
     1981 gctagatgta gctcgtactt atattccaat ggagtattta caagaaatga ctatttatat
     2041 ggcatactat aaacttaatg aagttcaagt tcatgtaaat gattattggg gagctacagg
     2101 gtattcagct tttagattag agtgcgatac ttatccaatg attacagcta ctgatggcag
     2161 ctacactaaa gaggagtatc gtcaatatca gattgacatg aaaaactatg gaatcaatgt
     2221 aattacggaa attgatacgc catatcatgc tgaatgtttt agaaatgttc ctggagtaaa
     2281 aatgcttaaa gcaggggcgt tggatattag agaacaatcc tcttatgatg ttattgaagc
     2341 tgttctggat gaatatttag atgggccaaa tcctgttatt caaagtgata aattccatat
     2401 tggaacagat gaatatgata aatcatattc agagcaaatg cgtaaatgga ctgatcattt
     2461 tattaattat gttaatgata aaggatatga tacaagatta tgggggtctt taggtagtcg
     2521 aggatttaat ggaacaacac cagtatcaaa tgatgctacg gttaacttat gggcaccata
     2581 ttgggcagat gttaaagaaa cttataatgc cggttatgat atcatcaata cttgtggggg
     2641 atggttatat attgttcctg gagctaatgc tggttatcca gatcgtttgg
//
LOCUS       DAOMTA010000388         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1670_length_2685_cov_2.526236, whole genome shotgun sequence.
ACCESSION   DAOMTA010000388 DAOMTA010000000
VERSION     DAOMTA010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1670_length_2685_cov_2.526236"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..555
                     /locus_tag="PLK68_10210"
     CDS             184..555
                     /locus_tag="PLK68_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90817.1"
                     /translation="MRYEDSMKGVADQIIKEQQRVSKIKNNPEEFHWHDEYATLNFFR
                     FICKNIGNLRNGEIEKMVTRLKNIDQKAVENHVNGAVWAFDYDKMFCVLMENEICRKV
                     CEKNKYNSWMNLIGQYCVSRT"
     gene            832..996
                     /locus_tag="PLK68_10215"
     CDS             832..996
                     /locus_tag="PLK68_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRM90818.1"
                     /translation="MKKYECEPCGYIYDPAVGDPDAGIDPETAFEDIPDDWTCPICGL
                     GKDVFVPVED"
     gene            1004..1507
                     /locus_tag="PLK68_10220"
     CDS             1004..1507
                     /locus_tag="PLK68_10220"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HRM90819.1"
                     /translation="MQVRNCQTLKEPKRTYKRNGGQIMTYDLNITFDDNLVTGNETID
                     TQHKELIDRIQNFVTACQNGDSKVKAIKMLDYLDEYTDFHFKEEEKLQEKSGYPEREK
                     HYEKHEEFRKTIQELYEYLQEYEGPTDRFSELVQKNVIDWLFGHIKTYDRSVAKFIFM
                     KQNPDRC"
     gene            1890..2318
                     /locus_tag="PLK68_10225"
     CDS             1890..2318
                     /locus_tag="PLK68_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90820.1"
                     /translation="MKKKIIAVISGTAILIIAAGSIYGKFESSHKEGEPDVVGTFSVN
                     RDENITVVANREDIEDREAFARKLLQMYKDDSFHSTKFSTDRGYATSIDMNIYLWKED
                     IEDGESVMTAEYRPVEYGKDYDVVNNPDKFQLYIDGKEIK"
     gene            2498..>2685
                     /locus_tag="PLK68_10230"
     CDS             2498..>2685
                     /locus_tag="PLK68_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6219 family protein"
                     /protein_id="HRM90821.1"
                     /translation="MKAHKYWSVGALITMLGTFYTGYKKQKSSHKYFALSSMLCMVMA
                     IYTGHKMITGNRKEKAKK"
BASE COUNT         1041 a          358 c          554 g          732 t
ORIGIN      
        1 cattacaata agacagatgt ggaagtgcag caggaactgg gaataggcag gacttctttt
       61 tataagatga agaagcaggc ccttggatat ttaggatttt atttttatga gatcgtggta
      121 ccgcaggcaa aggataaaag atttaaaccg tcactgggcg ttgaggaaga gtaggtgagt
      181 aatatgagat acgaagattc gatgaaaggt gtagctgatc agataatcaa agaacagcag
      241 cgagtcagta aaattaaaaa caatccagaa gagttccatt ggcatgacga atatgcaacg
      301 ttgaatttct ttcggtttat ctgcaaaaat atcggtaact tgagaaatgg agaaattgaa
      361 aaaatggtca cacgtttaaa gaacatagat cagaaagcag tggaaaacca tgtgaatggt
      421 gctgtttggg catttgatta tgataagatg ttctgtgtat tgatggaaaa tgaaatttgc
      481 cgaaaggtgt gtgaaaagaa taaatataac agttggatga atctgattgg acagtattgt
      541 gtttcaagga cttaaagacg aaaaaagttg ttggagagaa ataatttcca gcaactctgt
      601 ctttaatgaa tagagttcat ttttttattc aactattccc atgatatata caatatgtaa
      661 atttgcaatt caaatatcag tttatgctaa gtacaataat aataataatt actattattg
      721 ataagagaat aattatcatg tataatacag atatacaaga aacaaagtgt tagaaaaatt
      781 gctaggaact tggtaacaaa aataaatcga cattgaaaag ggaaaatgac tatgaaaaaa
      841 tatgaatgtg aaccatgtgg atatatttat gatccggcag taggagatcc agacgctggt
      901 atcgatccag aaactgcatt tgaggatatt ccagatgact ggacatgccc aatctgtgga
      961 ttaggaaaag atgttttcgt tcctgtagaa gactaagcag aaaatgcagg tacgaaactg
     1021 ccagacatta aaagagccta agagaacata taaaagaaat ggaggtcaaa ttatgactta
     1081 cgatttaaac attacctttg atgacaattt agtaacagga aatgaaacaa ttgataccca
     1141 gcataaagaa ttgatcgacc gtatccagaa ctttgtaact gcctgtcaaa atggcgacag
     1201 caaagtaaaa gcaatcaaaa tgctggatta tctggatgaa tatactgact ttcatttcaa
     1261 agaagaggaa aagcttcagg aaaaatccgg ttatccagag cgtgaaaaac attatgaaaa
     1321 acatgaagag tttagaaaga caattcagga actgtatgaa taccttcagg agtatgaagg
     1381 accgacagat cggttcagtg aacttgtaca gaaaaatgta atcgactggc tgtttggaca
     1441 cattaaaaca tacgaccgct ctgtggcaaa atttatcttt atgaaacaaa atccagaccg
     1501 atgctaaaat aaaccaggga acgagttcat tcagaatgta gacaacgtga tgaagtaaac
     1561 taggggctgt cacaatgcag cccctttcct gttatagcga tattctaggt aatatattgc
     1621 gctggcatga catattattt tactactact atatgggaag agcaagcagc tcttcttttt
     1681 ttgtcctttt tatccaatta taagttgtaa agtctctttt catgccaaca gaaatatgct
     1741 aaattgattt tatagaacaa gatataattc cacttatata tcggaggaaa atgaatgaga
     1801 gagaaaacta tatatgagaa gattgcagaa aagtataaca caacaccaga agaagtacgc
     1861 agtaaaaaaa ttaaaaggaa attaaaaata tgaagaaaaa gataattgca gtaatttcag
     1921 gaacagcaat cttgataatt gctgcaggaa gtatttatgg gaaatttgaa tccagtcata
     1981 aagaaggcga accagatgta gttggaactt tttctgtaaa tcgggatgaa aatataaccg
     2041 tggtcgcaaa tagggaagat attgaggata gagaagcgtt tgcaagaaaa cttttgcaga
     2101 tgtacaagga tgattctttt cattcgacaa aattctccac agacagagga tatgcgacga
     2161 gcattgatat gaacatctat ttatggaaag aggatattga agacggggaa tcggtaatga
     2221 cagccgaata tagacctgta gaatacggaa aggactatga tgttgtcaat aatcctgata
     2281 aatttcaact atatatagat ggaaaagaga ttaaatagta atctcctgaa agcaagcttt
     2341 ttgtcaatta tttcaaagaa aaattaaagg ataacaatac atagttttga caatgatatt
     2401 ttgaaaaatt ttataggaat gctctattat caaattgaca taggaatatg gtagcgtata
     2461 attagaattg aatacactga aaaaatggag gaattacatg aaagcacata aatattggtc
     2521 agtaggagca ttgatcacaa tgcttgggac tttttacact ggatataaaa agcaaaaatc
     2581 aagtcacaaa tactttgctt taagttccat gttatgtatg gtaatggcga tatatacagg
     2641 tcataaaatg attaccggga ataggaaaga aaaggcaaaa aaaga
//
LOCUS       DAOMTA010000389         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1671_length_2685_cov_2.123954, whole genome shotgun sequence.
ACCESSION   DAOMTA010000389 DAOMTA010000000
VERSION     DAOMTA010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_2685_cov_2.123954"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..2507)
                     /locus_tag="PLK68_10235"
     CDS             complement(36..2507)
                     /locus_tag="PLK68_10235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM90822.1"
                     /translation="MKLKYSLKGLDCANCAQKVQERVSKPENVRECSVVFATTKMFVE
                     TDTDLFDESKIVEAVKSVEPDAEVINLSKNGQDYNVSNNHEECNGEHDHKHHHDHEKC
                     GCGHEHEHHHDHKECRCGHDHEHNIESVQHGDLKGAINIKISGLDCANCAMKVEQAIN
                     KMNEIDEAMIIFSTETLKVKPKTSIAQNELLKKLQKVVDQVEDDVTLTLKDEIKVVEK
                     PKLFVPKEHLGLIGGTLVYIAGIIAGEFDYAAIVYGIAYLLVGYKVILKALKNIRRGE
                     VFDENFLMCIATIGAFCISDYKEAIAVMLFYSVGEIFQAYAVNKTRTSISSLMDLKSD
                     YANLLVGEEIKKVAPEEIKIGDEIIVKVGEKVPLDGVVLEGASTLDTSSLTGETLPRN
                     VSKGDEVLAGVVNLTGIINLKVSQVYEDSTVSRILDLVENAASKKAPIEQFITRFARI
                     YTPTVVFLAVALAIVPMLIFKDAVFTDWLYRALTFLVVSCPCALVISIPLGLYAGLGK
                     ASKVGALIKGGNYLELLKDIDTVVFDKTGTLTEGSFEVVEINGADDLLMLGAYGESMS
                     NHPIAKSILRKYGQEIDQKRISDFKEIAGKGIEVKIDDKVYNLGNKSYIEGLGITVNN
                     PSTVGTVVHIVCQGKYLGNIVVADKIKETTIEGIKHLKKYGIKNTVMLTGDRSEVAED
                     IAKKIGIDTVYSELLPQDKVIQVETLINQGAKLSFVGDGINDAPVLARADLGIAMGGV
                     GSDAAIEAADIVLMNDDIVTIGEAISISQKTNKILKQNVTFTLIIKIGVLLLTMFGYA
                     NMWMGVFADVGVTLIAILNSMRILR"
     gene            complement(2521..>2685)
                     /locus_tag="PLK68_10240"
     CDS             complement(2521..>2685)
                     /locus_tag="PLK68_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM90823.1"
                     /translation="ACLDMTQSAISHQLRILKQANLVKFRRDGKLMYYSLDDEHVKQI
                     FDAGYKHVIE"
BASE COUNT          861 a          570 c          309 g          945 t
ORIGIN      
        1 aagaaaatta ggaccggcta accagtccta atcaattatc gtaatattct cattgaattt
       61 aagattgcaa tcaatgttac tccaacatca gcaaacacac ccatccacat attggcgtat
      121 ccaaacattg ttaataataa gactccaatt ttaataatca atgtaaaagt cacattttgt
      181 tttagaattt tatttgtttt ttgtgaaata ctgattgctt caccaatcgt aacaatatca
      241 tcattcatca atacaatatc agctgcttca atagcggcat cacttccaac accacccata
      301 gcaattccta aatcagctcg agctagaact ggtgcatcat taataccatc accgacaaat
      361 gataattttg ctccctgatt gattagagtt tctacttgaa ttactttatc ttgtggcaac
      421 aactcactat aaactgtgtc aattccaatt tttttagcaa tatcttcggc tacttcgcta
      481 cgatcccctg tcaacattac agtattttta ataccatatt ttttcagatg tttgattcct
      541 tcaatagtag tttcttttat tttatctgcg acaacaatat tacctaaata tttaccttga
      601 caaacaatat gcactactgt ccctactgta gatggattat tgactgtaat tcctaaacct
      661 tcaatataag atttattacc taaattatat actttatcat caattttaac ttcaatacct
      721 ttacctgcta tctctttaaa gtcactaatt cgcttttgat caatttcttg accatatttt
      781 cttaaaatac ttttagcaat tggatgattt gacatacttt caccatatgc tccaagcatc
      841 aataagtcat cggcaccatt aatttctaca acttcaaagc taccttctgt aagtgttcca
      901 gttttatcaa acacaacagt atcaatatcc tttaataatt caagataatt accgcctttg
      961 attaatgccc caactttact tgctttccct aatcctgcat atagtcccaa tggaattgaa
     1021 ataaccaatg cacatggaca tgagacaact aagaaagtta aagcccgata taaccaatct
     1081 gtaaatactg catctttaaa aatcagcatt ggaacaattg caagtgcaac agccaaaaat
     1141 acaacagttg gcgtatagat tcttgcaaag cgtgtaataa attgttcaat tggagctttt
     1201 ttactagctg cattttcaac taaatctaaa attcttgaaa cagttgaatc ttcataaacc
     1261 tgagatactt taagattaat aattcctgtt aaattaacta cgcctgctaa tacttcgtca
     1321 cctttgctaa cattacgcgg cagtgtttca ccagttaaac tagatgtatc tagagtacta
     1381 gcaccttcaa gtacaactcc atcaagaggt accttttcac caactttaac aataatctcg
     1441 tcaccaatct taatttcttc tggagcaact ttttttattt cctcacccac taataaatta
     1501 gcataatcac tttttaaatc cattaatgaa ctaattgaag ttcttgtttt attaactgca
     1561 tatgcttgaa aaatttctcc aacagaatag aataacataa ctgcaatagc ttctttatag
     1621 tcactgatac aaaatgctcc aatcgttgca atacacatta agaaattttc atcaaaaacc
     1681 tcaccacgcc tgatattctt taatgctttt aatataactt tataccctac taataaatat
     1741 gcaattccat aaacaattgc tgcataatca aactcgcccg ctataatacc agcaatataa
     1801 actaaggttc caccaattaa cccaagatgt tccttaggaa caaataattt tggtttttca
     1861 actactttaa tttcatcttt taatgtcaaa gtcacatcgt cttcaacttg atcaactact
     1921 ttttgtagtt ttttcaataa ttcattttga gcaatcgatg tcttaggttt tactttcagt
     1981 gtttcagtag aaaaaataat cattgcttca tcgatttcat tcattttatt aattgcttgt
     2041 tcaactttca tagcgcaatt tgcacaatcc aatccactga ttttaatatt aatcgcacct
     2101 tttaaatcac catgttgaac tgattcaata ttatgctcat ggtcatgtcc acagcggcat
     2161 tccttgtgat catggtgatg ttcatgctcg tgtccgcagc cacatttctc gtgatcatga
     2221 tgatgtttgt gatcatgttc accgttacat tcttcgtgat tattagaaac attataatct
     2281 tgaccatttt ttgataaatt gattacttct gcatccggtt caacgctttt aacagcttca
     2341 acaatttttg attcatcaaa caaatcagta tctgtttcaa caaacatttt tgtcgttgcg
     2401 aacacaacac tgcactctct aacattttca ggtttactaa ctcgctcttg aaccttttga
     2461 gcacaattag cacaatctaa acctttcaaa ctatacttaa gtttcataaa taccctctcc
     2521 ttactcaatt acatgtttat acccagcgtc aaatatctgc ttaacgtgtt catcatcaag
     2581 tgaataatac atcaatttcc catctctcct aaatttaact aggtttgctt gctttaaaat
     2641 tcttaattgg tgtgaaatag cagactgtgt catatctaaa cacgc
//
LOCUS       DAOMTA010000390         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1673_length_2683_cov_2.381659, whole genome shotgun sequence.
ACCESSION   DAOMTA010000390 DAOMTA010000000
VERSION     DAOMTA010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_2683_cov_2.381659"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLK68_10245"
     CDS             complement(<1..475)
                     /locus_tag="PLK68_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRM90824.1"
                     /translation="MANREYPHDIEAERSLLGSMLISKDKCIDILNKTVEDDFYDDSH
                     QAIFKAMAAINEEGTPVDVTTVTSYLMDHSQLDKIGGVDYLLRLSESVPTVAHSEYYL
                     KILHNKATLRRIIRETTQIAENAYGDVENIDAFIDETEKTILKVTQDRSAGEFRDI"
     gene            complement(485..931)
                     /gene="rplI"
                     /locus_tag="PLK68_10250"
     CDS             complement(485..931)
                     /gene="rplI"
                     /locus_tag="PLK68_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRM90825.1"
                     /translation="MKVILLQDVKKVGKKGEVVKVADGYGQNFLIKNKLAVLETNTSR
                     KIVESQKEAEHQQDLENQAKAKELAKEIESITLEFTLKSGKDGKTFGSVSTKQVVEQL
                     REKYGIKIDKRKFIDAHPIGALGYTKLKVDLYKGIIATINVHLKER"
     gene            complement(931..>2683)
                     /locus_tag="PLK68_10255"
     CDS             complement(931..>2683)
                     /locus_tag="PLK68_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRM90826.1"
                     /translation="LNNEAKNAFIFGGIGLIKYDENRNISWVSDLFTEMKLNIVGKKL
                     LEWQPLLASLFEDDDIKVIDINSRKFEVYNSKESRLLYLKDVSDYVGISKEFEDQQVC
                     VAYITVDNYEESIEQADEQTAASIQSTTRQIVLDWAKENGIVLKRYKSDGYIAMFNER
                     TYRKQVEDKFKILDYFKEQAEQLGQMMTLSIGIGRGSNILRELDELAFSALSLAYSRG
                     GDQATVKSNDEPIRFFGGNSESYEKSNKIRARVIAQSLAGLIRQANNVLIMGHKQSDF
                     DSFGASIAMYSICKAYGKKAHIIIDYDSLEEKTGVIARSLRDDERYRGVFITPARINE
                     FNHSKTLLVNVDNHKPSLAIDANALDIIKNKVVIDHHRRGEEFIELPLLTYLEPAASS
                     TVELIVELFDYQKENVCVTEREATIMYAGMLIDTNYFRTRVGTRTFQAAAKLKEMQAN
                     VSEAYKYLEDDYDTTLTKLSITQTAYRYGENILIAFGRQDKIYSRTLLAKAGNELLGI
                     SGVKAVFTVGRTGKEEVSISARSTRDVNVQLIMEKLGGGGHFSMAACQLKYEDVTIAI
                     NLLEEAINEYLDERTNE"
BASE COUNT          827 a          566 c          322 g          968 t
ORIGIN      
        1 gaatatctct aaattcacca gcacttcgat cttgagttac ttttaaaatc gttttttcag
       61 tttcatcaat aaaagcatca atattttcaa catcaccata agcattttca gcaatttgag
      121 ttgtctcgcg aataattctt cgcaatgttg ctttattatg taatatcttc aaataatatt
      181 cactatgcgc tacagttggt acagattcac ttaatctaag caaataatca acaccaccta
      241 ttttatctag ctggctatga tccataagat aagatgttac cgtggtaaca tctactggag
      301 tgccttcttc attgattgca gccattgcct taaagattgc ttgatgactg tcatcataaa
      361 aatcatcttc aactgtttta ttcaagatat caatacactt atcttttgaa ataagcattg
      421 aacctaataa tgatctttct gcctctatgt catgaggata ttctcgattg gccatacatt
      481 tctcctatcg ttcttttaaa tgaacattga ttgttgcaat aattccttta tataaatcta
      541 cttttaattt agtatatcct aatgctccaa ttggatgagc atcaataaat tttcttttat
      601 caattttaat accatacttt tctcttaatt gttcaactac ttgtttagta gaaacgctac
      661 caaaagtttt accatcttta cctgatttta aagtaaattc taaagtaatg ctttcaattt
      721 ctttagctaa ttctttagct tttgcttgat tttctaaatc ttgttggtgc tctgcttctt
      781 tttgactctc aacaatttta cgacttgtat ttgtttctag aactgctaac ttgtttttaa
      841 ttaagaagtt ttgtccatac ccatcggcta ctttaacaac ttctcctttt ttaccaactt
      901 tttttacatc ttgtaataaa ataactttca ttattcattc gtcctttcat ctaaatattc
      961 gttgattgct tcctctaata aattaattgc aatcgttaca tcttcatact ttaattggca
     1021 agctgccata gaaaaatgac cgcctccacc taatttttcc ataattaact gcacattaac
     1081 atcacgggta cttcgtgcac tgatagatac ctcttcttta cctgtacgac ctactgtaaa
     1141 taccgcctta actccactaa tacctaatag ctcattacca gcctttgcta acaatgttct
     1201 actatatatt ttatcttgac gtccaaaagc aatcaagata ttttcaccat aacgatatgc
     1261 tgtttgcgta attgacaatt ttgttagagt cgtatcataa tcatcttcta gatatttata
     1321 agcttctgat acattcgctt gcatctcttt taatttagct gcagcctgaa atgttctagt
     1381 tcctacacgt gtcctaaaat aattagtatc gatcaacatt cccgcataca tgattgtcgc
     1441 ttcacgttca gtcacacaaa cattttcttt ttgataatca aataattcta caatcaactc
     1501 aacagttgac gaagctgccg gctctaaata cgtcagcaat ggtaattcaa taaattcctc
     1561 accacgacga tgatgatcaa ttacaacttt atttttaatt atatcaagtg cattagcatc
     1621 gattgctaat gacggtttat gattatcaac attcaccagc aaggttttac tgtgattaaa
     1681 ttcattaatt ctagccggtg taataaaaac tccacgataa cgttcatcat cacgcaggct
     1741 ccgagcaatt accccagtct tttcttccaa tgaatcataa tcaataatga tgtgtgcctt
     1801 tttcccatag gctttacaaa ttgaatacat tgcgattgaa gcaccaaaag aatcaaaatc
     1861 cgattgttta tgccccataa ttaaaacatt attagcctga cgaatcaatc ccgctaatga
     1921 ttgagcaatt acacgggcac gaattttatt tgatttttca tagctttcac tatttccacc
     1981 aaagaaacga attggttcat catttgattt aactgtagcc tgatctccgc ctcgagaata
     2041 agccagcgaa agcgcagaaa aagccagctc atcaagttca cggagaatat tacttcctcg
     2101 tccaattcca atagataatg tcatcatttg tcctaactgc tctgcttgtt ctttaaaata
     2161 atccaagatc ttaaacttat cttcaacctg tttacgataa gtcctttcgt taaacattgc
     2221 aatataacca tcacttttat agcgtttcaa aacaattcca ttttcttttg cccaatctaa
     2281 tacgatctgg cgtgtcgttg attgaattga agcagctgtt tgttcatcag cctgttcaat
     2341 cgactcttca tagttatcaa ccgtaatata cgcaacacaa acctgctgat cttcaaactc
     2401 tttactaatc cctacataat cacttacatc ttttaaatat aataaacggc tttccttact
     2461 attataaacc tcaaatttac gagaattgat atcaatcacc ttaatatcat catcttcaaa
     2521 taaagaagct aataacggtt gccattctaa taatttttta ccaacaatat ttaatttcat
     2581 ctcagtaaat aaatcactga cccaagaaat attccggttt tcatcatatt taatcaggcc
     2641 gattccacca aaaataaagg catttttagc ttcattattt aac
//
LOCUS       DAOMTA010000391         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1676_length_2682_cov_2.019414, whole genome shotgun sequence.
ACCESSION   DAOMTA010000391 DAOMTA010000000
VERSION     DAOMTA010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1676_length_2682_cov_2.019414"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..280
                     /locus_tag="PLK68_10260"
     CDS             23..280
                     /locus_tag="PLK68_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90827.1"
                     /translation="MGLLEKIADIMQCTYISDLRGRLSLDHEQLQFLNELRAETYALS
                     EWQEAVRYITGNLDSFNSAAEGKNILLDYYIKKEATEISIK"
     regulatory      319..420
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            495..>782
                     /locus_tag="PLK68_10265"
     CDS             495..>782
                     /locus_tag="PLK68_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRM90828.1"
                     /translation="MKNKTFKITTKTIAYMAMFMALQVVLELAFKVVPGQPQGGSITL
                     SLVPILLASYLLGGYYGIVVGACCCGLHFVLGLATYWGPWSLFLDYLIPLAV"
     assembly_gap    783..792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <793..1047
                     /locus_tag="PLK68_10270"
     CDS             <793..1047
                     /locus_tag="PLK68_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRM90829.1"
                     /translation="IASFFKNFEIKGHVVYPGIIVVMILKFISHYLSGAWLFGAYAPE
                     GMNPWWYSFGYNLAYCLPTLIICYIGFALIYPRLNTSIKL"
     gene            1142..>1454
                     /locus_tag="PLK68_10275"
     CDS             1142..>1454
                     /locus_tag="PLK68_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HRM90830.1"
                     /translation="MSDSLVGKKVLIHCYKHDGSIHRCWAKGFILDENDDHFIAINNR
                     TLVTESDGRMWHTREPAICYFPKNKWYNVICMIRKTGVYYYCNIASPTLYDGEALKYI
                     DY"
     assembly_gap    1455..1472
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1473..1690
                     /locus_tag="PLK68_10280"
     CDS             <1473..1690
                     /locus_tag="PLK68_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90831.1"
                     /translation="DLKVFPDNNYRVLDEEEYKQHAKQMNYSKELDRILKKQLELLID
                     LATKREGPFSEDFTKHWYHVYEDLTQG"
     gene            1700..2149
                     /gene="tsaE"
                     /locus_tag="PLK68_10285"
     CDS             1700..2149
                     /gene="tsaE"
                     /locus_tag="PLK68_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRM90832.1"
                     /translation="MEKVIKVNNLEETIALGNRLGLLLQPNMLLTLSGDLGAGKTTFT
                     KGIGQGLGITKVINSPTFTILKQYQGRLNLSHFDAYRLEGQDDDLGFEEIFDSDDVCV
                     VEWANFIEDILPVDRLTIEIKKIDENIREFVFKTNSEKYAQVVEALT"
     gene            2146..>2682
                     /gene="tsaB"
                     /locus_tag="PLK68_10290"
     CDS             2146..>2682
                     /gene="tsaB"
                     /locus_tag="PLK68_10290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRM90833.1"
                     /translation="MKTIVMDTSNAYLVIGLYEDDQCIDKYQADGNRRQSEYALTHLD
                     EMLKKHHWEVLNVDEMIITIGPGSYTGQRVALTIAKTLAAISKIKIKAVSSLHGYAGA
                     SKAISVIDARSKKIFVGVYEHNQAIIDDQIMLIDDFANFKEQYPDFQVIGDSDLVGIN
                     KVKLDLSDCIYQAGKSVDY"
BASE COUNT          894 a          336 c          536 g          888 t
ORIGIN      
        1 attttagaaa aggagaaaag cgatgggtct acttgaaaaa atagccgata ttatgcagtg
       61 tacctatatt tctgatttaa gaggaagatt atcgcttgac catgaacagt tacagttttt
      121 aaatgaatta agagctgaaa catacgcttt gtctgaatgg caagaagcag taaggtatat
      181 tactggtaat ttagattctt ttaattcggc agcagaagga aaaaacatct tattggatta
      241 ctatattaaa aaagaagcta cagaaatatc aataaaatga tttttttctt gatattataa
      301 taagataatc tataatagta tacgagccag gggtgcaaaa ttgctgagaa gggcataagc
      361 ctgatccctt atcatctgaa ctgggtaatg ccagcgtaga gagagcaaat ttaaaattgt
      421 ttgctttttt gcgttcgcaa agaagcattt tttaattttg ttaggctctt tcataaggaa
      481 ggggaaaaac aaatatgaaa aacaaaacat ttaagattac aaccaaaaca attgcttata
      541 tggcgatgtt tatggcttta caagtagttt tagaattagc ttttaaagtc gttccgggac
      601 agcctcaggg aggaagtatt acgctgtcat tagtaccgat cctattagcc tcatatttat
      661 taggtggata ttacggtatt gttgttggag cctgctgctg tggactgcat tttgttttag
      721 ggttggctac atattggggg ccatggtcat tatttttaga ttatttaatt ccactagctg
      781 tannnnnnnn nnatagcatc attttttaaa aactttgaga ttaaaggtca tgtagtttat
      841 cctggaatta ttgttgtaat gattcttaag tttatctcac attatttatc tggagcatgg
      901 ctgtttggag catatgctcc tgagggaatg aatccatggt ggtattcatt tggttataat
      961 ttagcctatt gcctaccaac tttaattatt tgctacatcg gatttgcctt aatatatcca
     1021 cgattaaata cttcgattaa actgtaaaag aaaagtagct tgctactttt cttttcttct
     1081 gtctttaaaa aggatagtaa tttgtgtata atagtactgt tataaagaga ggtagtaaaa
     1141 gatgtcagat tctctggtag gaaaaaaagt attaattcac tgttataagc acgatggaag
     1201 tattcatcga tgctgggcca aaggattcat cttagatgaa aatgatgatc attttattgc
     1261 aatcaataat cgcaccttag taactgaatc tgatggtcga atgtggcata caagagagcc
     1321 tgccatctgt tattttccta aaaataagtg gtacaatgtg atttgtatga ttcgtaaaac
     1381 aggagtttat tattattgta atattgcttc accaacttta tatgatggtg aggcattgaa
     1441 atatattgat tatgnnnnnn nnnnnnnnnn nntagattta aaagtattcc cagataataa
     1501 ctatcgggtt ttagatgaag aagagtataa gcaacatgct aaacagatga attattcaaa
     1561 agaattggat agaatattaa aaaaacaatt agaactgcta attgatttag caacgaaacg
     1621 tgaaggaccg tttagtgaag attttacgaa acattggtat catgtatatg aagatttgac
     1681 acaaggatag gagaatacaa tggaaaaagt aattaaagta aataaccttg aagaaacgat
     1741 tgctttgggt aatcgtttag gcttgttatt acaaccaaat atgctattga ctttaagtgg
     1801 cgacttagga gccgggaaga cgacctttac caaaggaatt ggtcaaggat tagggattac
     1861 caaggttatt aatagtccta cttttaccat tttaaaacaa tatcaaggtc gcttgaattt
     1921 gtcacatttt gatgcttatc gtttagaagg acaggatgat gatttaggat ttgaagaaat
     1981 ttttgatagt gatgatgttt gtgttgttga atgggctaat tttattgaag atattttacc
     2041 ggtagaccgt ttaacaattg aaataaaaaa aattgatgaa aatattcgcg aatttgtctt
     2101 taaaacaaat agtgaaaaat acgctcaagt tgtggaggct ttaacatgaa gacaattgta
     2161 atggatacat caaatgctta tttagttatt ggcttatacg aagatgatca atgtattgat
     2221 aagtatcaag cagatggaaa tcggcggcaa tctgagtacg ctttaacaca tcttgatgaa
     2281 atgttgaaaa aacatcattg ggaagtttta aatgtggatg aaatgataat tacgattggt
     2341 cctggttctt atactggtca acgggtcgct ttgacgattg ccaaaacttt ggcagcaatt
     2401 tcaaaaataa aaatcaaggc agtttcttcg ttacatggtt atgctggagc ttctaaggca
     2461 atcagtgtaa ttgatgctcg aagcaaaaag atttttgttg gggtatatga gcataatcag
     2521 gccataattg atgatcaaat catgttgatt gatgattttg ctaattttaa agaacaatat
     2581 cctgattttc aagtgattgg tgatagtgat ttagtcggaa ttaacaaagt taaactcgat
     2641 ttaagtgatt gtatttatca agctggtaaa agtgttgatt at
//
LOCUS       DAOMTA010000392         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1683_length_2676_cov_2.046166, whole genome shotgun sequence.
ACCESSION   DAOMTA010000392 DAOMTA010000000
VERSION     DAOMTA010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_2676_cov_2.046166"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLK68_10295"
     CDS             complement(<1..1134)
                     /locus_tag="PLK68_10295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM90834.1"
                     /translation="MDLATEKLHILKSVLPTNNDHDQKIFSLKQFLLLLEKRLIINFE
                     KKELENFLTIDNLRLLSKTQKTSHLDLRFQKELENYEWVQLKTLYLPKNNEKIYLVFS
                     NVDREHIINSITEKFVYKKGDYLYYLDTKNGYFSNFSITDNGTVIPPQEGNNYTQAMI
                     KYTRQNTVLEDQDRVIEQLTPEYILNRLQEQSVYKIELGMLDENKEYRRKLITVQYYD
                     EENKIVLIRRSDITKEYLKQKNQENKLKAIKKAADTDALTGIYNRIGAQRLIVEYLHK
                     SNNEMSAFIIIDLDNFKKINDSLGHLQGDEVLKQVANILKENFRKTDIIARLGGDEFI
                     VFMKNIVQKENAIITLNNLLKKLRLSYQWHEETILITGSIGVAV"
     gene            complement(1136..1996)
                     /locus_tag="PLK68_10300"
     CDS             complement(1136..1996)
                     /locus_tag="PLK68_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90835.1"
                     /translation="MENLNIREDVSDIVNKTELLADLQIGFWIIEYPNDGIPKMYGDT
                     IMYSLLGGNSNTLTPEELYSFCMERIDSEYIGLVFDAIENIKKNKRTEIRYPWRHEEK
                     GWTWVRCGGFLDKSYKNGIRFKGYYHDITEELEMDIKNNEHKIIDFKKLRLYSPYFIE
                     NCEGLFEIDIDSLEIKTIFYKKKKYHKIDDGDNVFSVIRKWTHPNDITRLDNIFVSAS
                     LEQIINKKKIKQIEFKTKTIFGDYTWVEARIFCAQVMGIKKLLFYIYDISDRKITDYI
                     PKGDYIFYNC"
BASE COUNT          885 a          376 c          337 g         1078 t
ORIGIN      
        1 tacagcaact ccaatagaac cagttattag aattgtttct tcatgccatt gataagataa
       61 tcgtaatttt ttaagcaggt tatttaatgt tatgatggca ttttcttttt gaacaatatt
      121 tttcataaat acaataaatt catcaccacc caaacgagca attatatctg tttttcgaaa
      181 attttctttt aatatattag ctacctgctt taatacttca tcaccttgta aatgccctaa
      241 actatcattg attttcttaa aattatctaa gtcaatgata ataaaagcac tcatttcatt
      301 atttgattta tgaagatatt ctactattaa acgctgagca ccaatccggt tatagatacc
      361 tgtaagggca tctgtatcag ctgctttttt aatagctttt agcttgtttt cttgattttt
      421 ttgtttaaga tattcttttg taatgtcact tcttcgaata agtacgattt tattttcttc
      481 atcataatat tgaacagtaa ttaattttct ccgatattct ttattttcat ctaacatacc
      541 tagttcaatt ttataaacac tttgttcttg cagacgatta agtatatatt caggagtcaa
      601 ttgttcgata actctgtctt gatcttctaa aacggtattt tgtcgtgtgt attttatcat
      661 tgcttgagta taattattgc cttcttgagg aggaatgaca gtaccgttat cagttatcga
      721 aaaattagaa aaatagccat tttttgtatc aagataatac aaataatccc cttttttata
      781 aacaaatttt tctgtaattg aattaatgat atgttctcta tcgacattac taaatacaag
      841 atatattttt tcattgttct tgggaagata gagagttttt aattggaccc attcataatt
      901 ttctagttcc ttttggaaac gtaggtcaag atgtgatgtt ttttgtgttt tagaaagtaa
      961 tcttaaatta tcaattgtta aaaagttttc taattctttt ttttcaaagt tgataattaa
     1021 tcttttttca agtagcaaaa gaaattgttt tagtgaaaat attttttgat catgatcatt
     1081 attggtaggt aaaacagatt ttaatatatg taatttttca gtagctaagt ccacttcaac
     1141 aattgtagaa tatatagtcg ccctttggaa tataatcagt aatttttcta tcagaaatat
     1201 cataaatata aaataataat tttttaattc ccattacttg ggcacaaaat attctagctt
     1261 ctacccaagt ataatctcca aaaattgttt ttgttttaaa ctcaatttgt tttatctttt
     1321 ttttattgat tatttgttct agagatgcag atacaaaaat attatctaaa cgtgtaatat
     1381 cgtttgggtg tgtccatttt cttataacag aaaaaacatt atccccatca tcaattttat
     1441 gatatttttt ctttttataa aaaatagtct ttatttctaa actatctata tcaatttcaa
     1501 aaaggccttc acaattttcg ataaagtaag gtgaatataa tcgtaatttt ttgaaatcga
     1561 ttattttgtg ctcattattt ttgatatcca tctcaagttc ttctgttata tcatgataat
     1621 agcctttaaa tcttattcca tttttatatg atttatcaag aaaaccgcca caccgaaccc
     1681 atgtccaccc tttttcttca tgtctccatg gataccgtat ttcggttctt ttgttctttt
     1741 ttatattttc gattgcatca aaaactaatc ctatatattc agaatcaatt ctctccatac
     1801 aaaatgaata gagttcttcc ggcgtcagag tattgctgtt tcctcctaat aacgagtaca
     1861 taattgtatc accgtacatc tttggaattc cgtcgttagg atattctatt atccaaaaac
     1921 cgatttgcaa atctgctaat aattcggttt tattaacaat atctgataca tcttctctta
     1981 tattcaaatt ttccattatt taaaacctcc ccaatataaa tttgtatcaa ataatttgca
     2041 tattttttat catttttcag ttaaccatta tacaatatca atttctttta tgaaaggctc
     2101 ttttagttat atcagttgat aaaatgataa aaaagatttc aattttaaac cattatatca
     2161 aataaaaata attttttatt taccatgttt gtacgacttc acctattgga ttaagtagaa
     2221 aatataatta taaaataaaa atgatagttt actttaatag caagctgtag gcttatgaat
     2281 aatcatgata tgataaattt ggaagtaaga ttaattgtaa tagagttttt aattatgatt
     2341 gatatagatg gatattttac aattagcgat ggaataaata gggaataggg agactttttt
     2401 gaaaaatatt ttcttgtacg tataaataat agacataaat ttttttgata aggtaaaata
     2461 aatgtggttg agggaagaaa atcctttaaa atatgtgtaa aaaggagttg acagtcatgt
     2521 tcaaaactgc gatcctgcca gacagtagga tagtgtctgc ttttttgcat atatcattac
     2581 ttgttgcagc cggaattcta attactatat ttgttgtatt aaagaaaaag aaaggattaa
     2641 aaattcttag tttataaata taaatcagca tcctgc
//
LOCUS       DAOMTA010000393         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1684_length_2675_cov_2.767176, whole genome shotgun sequence.
ACCESSION   DAOMTA010000393 DAOMTA010000000
VERSION     DAOMTA010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1684_length_2675_cov_2.767176"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PLK68_10305"
     CDS             complement(<1..134)
                     /locus_tag="PLK68_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM90836.1"
                     /translation="MNILLVCAAGMSTSLLVNRMNEAAAAKGIGINIEAHPVGSIDQF
                     G"
     gene            complement(150..803)
                     /gene="rpe"
                     /locus_tag="PLK68_10310"
     CDS             complement(150..803)
                     /gene="rpe"
                     /locus_tag="PLK68_10310"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM90837.1"
                     /translation="MEKLLCPSMMCANFGNLEKEIKELEEGGIDFFHLDVMDGSYVPN
                     FGMGLQDIEYICKQATKPCDVHLMVVNPSDYIEKFAALGVKIIYIHPETDKHACRTLQ
                     KIKDAGAKAGIALNPGTSFETVKELLYLCDYVMLMSVNPGFAGQKYLDFVTPKFRQFV
                     DAASNYGGYQVMIDGACSPEKIAMLSKVGVKGFILGTSALFGKEKSYKEIITELRKL"
     gene            1004..2023
                     /locus_tag="PLK68_10315"
     CDS             1004..2023
                     /locus_tag="PLK68_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM90838.1"
                     /translation="MVKKKNISIKEIAKLADVSVATVSRVINNNGRFSDKTKEKVETI
                     IKEYGYTANIAAKSLRTSKSKTIGLIVPNIDNEWFSQLALDIENYFFKHNYSVFICNT
                     SQNEEKEVAYFKSLDSKLVDGIICISGIETIPSDSLTRDIPIVCIDRKPKDHSNAYYV
                     ESNHYLGGYLATEELIQQGCKNIAIVSRNKSLSVNKQRLMGYRQALKDYQLQENKQLE
                     LLLDIEQANYEGAKEAINQLIKNNIPFDGVFATNDWRAYGVLVGLLENNVKVPDEVKI
                     IGFDDIFISASSHPSLSTINQNIPALTKTACSLLLDLMNDIAINDEKKQFILPVEVIR
                     RDSTS"
     gene            complement(2157..2369)
                     /locus_tag="PLK68_10320"
     CDS             complement(2157..2369)
                     /locus_tag="PLK68_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90839.1"
                     /translation="MRCNEQSDFKYISNYAPSVIAEFNDNHLIIKDFNTRKDFYEFCQ
                     CKIDLWKENYNILDPKVGDLIENSFV"
BASE COUNT          914 a          489 c          348 g          924 t
ORIGIN      
        1 ccaaattgat cgatactccc aactggatgt gcttcaatat taatcccgat tccttttgct
       61 gcagccgctt cgttcattcg atttacaagt agacttgttg acattccagc tgcacatact
      121 aacaaaatat tcattttatc cttcctcctt tataactttc ttaattctgt aataatctct
      181 ttataacttt tttctttgcc aaacaacgca ctagttccta aaataaatcc tttaacgcct
      241 acctttgata acatcgcgat tttttctggt gaacaagcac catcgatcat tacctgatag
      301 ccgccatagt tagaggcagc atcaacaaac tgccggaatt ttggagtgac aaaatctaaa
      361 tatttttgtc ctgcaaaacc tgggttaaca ctcattagca tgacataatc acataaatac
      421 aaaagttctt taactgtttc aaaagatgtt cccggattca aagcaattcc tgcttttgct
      481 ccagcatctt taatcttttg taatgttcgg caagcatgct tatcagtctc tggatgaata
      541 taaataatct ttacgcctaa agctgcgaat ttctcaatat aatcacttgg gttgacgacc
      601 attagatgca catcacatgg tttcgttgct tgtttacata tatactcaat atcttgcaga
      661 cccatcccaa aatttggaac atagctacca tccataacat ctagatgaaa aaaatcaatc
      721 ccaccttctt ctaactcttt tatttccttt tcaagattgc caaaattagc acacatcatt
      781 gatggacata atagtttctc catacaatcc tcctatattt tgagtaaacg tttacctttc
      841 gttcatactc ctattatata tatcttgagt aaacgtttcc acatatatcc atggcacaat
      901 atgtgccgtt gtatttattc acctttttct ttgtgagtaa gcgattacat tttattgaaa
      961 atatctattt tttaatctat aatgaaaatg gaggggaaca aaaatggtaa agaaaaaaaa
     1021 tatttcaatt aaggaaatag ctaagttagc tgatgtctct gtcgccactg tttctagagt
     1081 tatcaacaat aatggccgct tttctgataa aactaaagag aaggtcgaaa caattattaa
     1141 agaatatggt tatactgcta atattgccgc caaaagctta cggacatcaa aatcaaaaac
     1201 aatcggatta attgttccaa acatcgataa tgaatggttt tcacagttag cattagatat
     1261 cgaaaactat ttttttaaac acaattattc agtatttatt tgtaatacct cacaaaatga
     1321 agaaaaagaa gttgcttatt tcaaatcact agattctaaa ttagtggacg gaatcatctg
     1381 catttcagga attgaaacaa tcccatcaga ttcattaact cgtgacatcc caattgtttg
     1441 tattgatcga aaacccaaag atcatagtaa tgcctactat gttgaatcta atcactattt
     1501 agggggatat ttagcaactg aagaactaat tcaacaaggg tgtaaaaata ttgcaatcgt
     1561 atcacgaaat aagtcattat ccgtcaacaa gcagcgttta atgggctatc gtcaagcttt
     1621 aaaagattat caattacaag aaaacaaaca gctagaatta ttattggata ttgaacaagc
     1681 taattacgaa ggggctaaag aagcaattaa tcaattaatt aaaaacaata ttccttttga
     1741 tggtgtcttt gcaactaatg attggcgtgc ttatggggta cttgtaggac ttttagaaaa
     1801 caatgttaaa gtccctgatg aagttaagat cattggtttt gatgatattt tcatttctgc
     1861 atccagccac ccatcacttt caacaatcaa tcagaacatt cccgctttaa ctaaaacagc
     1921 ttgtagttta cttttagatt taatgaatga tattgctatc aatgatgaaa aaaagcaatt
     1981 tattttgcca gttgaagtta ttcgacgaga ttcaactagt tgattataat actcttttaa
     2041 acttatacgt gatatgctcc cttttaaaga acaaataatc ttgttgttta gaagagagca
     2101 ttttatttaa ttaaaaagct agagatattt ctctaatttt taatggttta tttaaatcac
     2161 acaaacgaat tttcaattaa atcaccaacc ttaggatcta aaatattata attttctttc
     2221 cataaatcta ttttacactg acaaaactca tagaaatctt ttctagtatt aaagtctttg
     2281 attattaaat gattatcatt aaattccgca ataacagacg gtgcatagtt agagatatac
     2341 ttaaaatcac tttgctcatt acatcgcata aatgaatgaa aaatatttct tttcgctaac
     2401 tgaaaaaatc tgcactattc ataatccatt cccctttggt ctcaaataac attctatttt
     2461 agtatattaa actaattaaa ttcttctctt taagattatc tcactactat acattatctg
     2521 cgaattaaaa ttatgtctca tattatgcca atacttttaa atcaagtaat taaaaagagc
     2581 ccttcacaga cccttttaaa tatatttaat ctaatctttc accattacta gcaattacct
     2641 gcttatacca attaaaagat tttttctttc ttctt
//
LOCUS       DAOMTA010000394         2675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1685_length_2675_cov_2.163740, whole genome shotgun sequence.
ACCESSION   DAOMTA010000394 DAOMTA010000000
VERSION     DAOMTA010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_2675_cov_2.163740"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLK68_10325"
     CDS             complement(<1..218)
                     /locus_tag="PLK68_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90840.1"
                     /translation="MGFPKGFLWGGAIAANQAEGAYLEDGKGLTTVDMIPHGEKRMYV
                     KLGDMYPVKLIDGENYPSHEAIDFYHRY"
     gene            complement(278..1714)
                     /locus_tag="PLK68_10330"
     CDS             complement(278..1714)
                     /locus_tag="PLK68_10330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM90841.1"
                     /translation="MKKTVKLPENFFLGAAASAWQTEGWSEKKESQDSYIDLWYKENK
                     NVWHNGYGPAVATDYYHRYKEDIAYMKEIGMNCYRTSLNWSRFLTDYENIVVDEEYAN
                     YYDKMLDELIAQGIEPMVCLEHYEIPAELFKKYDGFASKRVVELFVKYAQEAFKRYSH
                     KVKYWFAFNEPVVVQTRIHLDALRYPFYQDSKAWMQWNYNKALATNMIMKVYKEGGYR
                     IAGGKFGTIINVETAYPRGNSPRDLEAADKYDLFYNRIFLDPAILGHYEEGFFDLLKK
                     HDILMEYTQEELEIIQNNTLDWVGINLYHPNRVKGRTTIVHPEAPFHPDFYYEEFNMP
                     GKKMNPHRGWEIYPQIMYDMAMRMKNDYHNFEWFVAESGMGVENEKQYKNESGMIQDD
                     YRIEFISMHLDWLLRGVTEGSNCKGYMLWAFTDNVSPMNAFKNRYGLVEIDLEDNRNR
                     RLKKSAYFYKEIIEKRSFEIETDEEVYK"
     gene            complement(1882..>2675)
                     /locus_tag="PLK68_10335"
     CDS             complement(1882..>2675)
                     /locus_tag="PLK68_10335"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRM90842.1"
                     /translation="NYKQTIRNLAKCGIKCICYNFMPVFDWTRSSVDHQLNDGSKALV
                     YYKNEVAQLDPTKLSLPGWDTSYSVMEVSELITAYKELGEEGLWENLKYFIKEILPVA
                     VECDVNMAIHPDDPPWSIFEIPRIITNEKNLDRFLSLYDDCHHGLTLCSGSLGCASFN
                     DIPLLIRKYGKMRRIHFAHIRNVKILDDGSFEESAHYSRCGSLDMVEIMRAYIEVGFE
                     GYIRPDHGRMIWGETGKPGYGLYDRGLGAMYLGGIIDALKDDGDL"
BASE COUNT          860 a          596 c          318 g          901 t
ORIGIN      
        1 tgataacgat gataaaaatc aattgcctca tggctaggat aattctcacc atctattaac
       61 ttaacaggat acatgtcacc taattttaca tacattcgct tttcaccatg cggaatcata
      121 tcaacggtcg ttaacccttt gccatcctca agataagctc cttcagcctg gttagcagcg
      181 attgctccac cccataaaaa accctttgga aatcccataa ccttctcctt tatcacttca
      241 agtattttca attctgataa cttagaaaaa atcagaacta tttataaacc tcttcatctg
      301 tttctatttc aaaactacgt ttttctataa tttctttata gaaataagct gatttcttta
      361 aacgcctatt tcggttgtct tctaaatcaa tttcaactaa gccatatcta tttttaaaag
      421 cattcattgg cgaaacatta tcagtaaatg cccatagcat atatccttta cagttagagc
      481 cttctgtaac tcctctaagc agccaatcca aatgcataga gataaattca atacgataat
      541 catcttgaat cataccagat tcatttttgt attgcttttc attctctacc cccataccac
      601 tttcggcaac aaaccattcg aaattatgat aatcattctt cattctcatg gccatatcat
      661 acatgatttg tggatagatt tcccagcctc gatgaggatt cattttcttt cctggcatat
      721 taaattcttc ataataaaaa tctggatgaa atggtgcttc tggatgaact atcgttgttc
      781 ttccttttac tcgatttgga tgatataaat taattcccac ccaatccaaa gtattatttt
      841 gaataatctc aagttcttct tgggtatatt ccattaaaat atcatgcttc ttcaataaat
      901 caaagaaacc ctcttcatag tgacctaaga ttgctggatc taaaaaaata cgattataaa
      961 ataagtcata tttatcagcc gcctctaaat cacgaggaga attccctctt ggataagctg
     1021 tctccacatt aataatagta ccaaacttcc caccggcaat ccgatatcca ccctctttat
     1081 aaactttcat aatcatattt gtcgctaaag ctttattata attccattgc atccaagctt
     1141 tactatcttg ataaaaagga tatcttagtg catcaagatg gattctagtt tgaactacga
     1201 ccggttcatt aaaagcaaac caatatttta ctttatgact atagcgctta aatgcttctt
     1261 gagcatattt cacaaataat tccaccacac gtttacttgc aaatccatca tattttttaa
     1321 ataattcagc aggtatttca taatgttcca aacataccat cggttcaatt ccttgagcaa
     1381 tcaattcatc cagcatttta tcatagtaat tcgcatattc ttcgtcaact acaatatttt
     1441 cataatccgt taaaaatcgt gaccagttta acgatgtcct ataacaattc atgccaatct
     1501 ctttcatata agcaatatct tccttatagc gatgataata gtcagtcgct accgcaggac
     1561 cataaccatt atgccaaaca tttttatttt ccttatacca taaatctata taagaatcct
     1621 gagactcctt tttctcactc cagccttctg tctgccaagc acttgcagct gcacctaaga
     1681 aaaaattctc tggtaatttt actgtttttt tcatattttt ctccatttcc ccacgctaag
     1741 cgcatggaca caaatatttc ttgaaaacat tctagttctt tctactcttc ctatccccct
     1801 gctataatga aagagaggtg gtatactaca gagcacgata aggagagcag gtaactctac
     1861 tcgttagaat atcctgtgta attataagtc gccgtcatct ttcaaagcat caataatccc
     1921 cccaagatac atagccccca aacctctatc gtataaacca tatcccggtt taccagtttc
     1981 tccccatatc attcgaccat gatccggtct tatatatcct tcaaaaccta cttcaatata
     2041 tgcacgcata atttccacca tatctaaaga cccacatctt gaatagtggg cactttcttc
     2101 aaaagaacca tcatctaata tttttacatt tctaatatgt gcaaaatgaa ttcttctcat
     2161 ttttccatat tttctaatta acaacggaat atcattaaaa gaagcacacc ctaatgatcc
     2221 actgcacaac gttaagccat gatggcaatc atcatataac gataaaaatc tatctaaatt
     2281 tttttcattt gtaataatac gtggaatctc aaaaatagac caaggcggat catcgggatg
     2341 aatggccata ttaacatcac actcaacagc aactggtaag atctctttta taaaatattt
     2401 aagattttcc cataatcctt cctcacccaa ctctttgtat gctgtaatta attcactcac
     2461 ttccattact gaataagaag tatcccaacc cggtaaagat agtttcgttg ggtctaattg
     2521 agcaacttca tttttgtaat agactaatgc tttcgaacca tcatttaatt gatgatcaac
     2581 agaagatcta gtccaatcaa aaacaggcat aaaattataa caaatgcatt taattccgca
     2641 tttagcaaga tttctaattg tttgtttata attat
//
LOCUS       DAOMTA010000395         2670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1691_length_2670_cov_2.287189, whole genome shotgun sequence.
ACCESSION   DAOMTA010000395 DAOMTA010000000
VERSION     DAOMTA010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_2670_cov_2.287189"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLK68_10340"
     CDS             complement(<1..619)
                     /locus_tag="PLK68_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM90843.1"
                     /translation="MVKAIGIVNLHSDVDFVGLTERRPVASVSFLGRYALIDFVLSNM
                     SNSTIDAVGVLIQKKPRSLFKHLGNGDSWNFNSKAGGVSLLYNEKYANNPNYNHDINN
                     LIENIAFLEANKADYVVIAPAHIITTMDYEDVIENHEKTGAEITMVYKKVHDANEAYI
                     GCDCLTIKDKVVTAVERNKGSRKDRSISLETYVINTKTLLKVMKQA"
     gene            complement(634..1758)
                     /locus_tag="PLK68_10345"
     CDS             complement(634..1758)
                     /locus_tag="PLK68_10345"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRM90844.1"
                     /translation="MGREIVAMILAGGRGTRLEALTAKVAKPAVHFGGKYRIIDFPLS
                     NCANSGIDIVGVLTQYESVLLGTYVGAGTKWGLDGKQSLAAILPARERGEVGATWYAG
                     TADAIYQNISFIDQYDPEYVLILSGDHIYKMDYDKMLTAHKQRKADATIAVLNVSLKE
                     ASRFGIMNTYEDGTIYEFDEKPEKPKSTLASMGIYIFTYKQLRKYLIADAKKEDSKHD
                     FGMNIIPDMLNDNKKLYAYEFDGYWKDVGTVESLWQANMDLLKDKDLDLYNIKKDWKI
                     YTEDTLGKPQIIGDQAKVKNSLITQGCLVNGNVEGSVLFNNVNVGEGAKVIDSVLMPG
                     VLVEEGAEVYKAIVDEGVVIRAKTKINKEAKEVALVSDNK"
     gene            1997..2416
                     /locus_tag="PLK68_10350"
     CDS             1997..2416
                     /locus_tag="PLK68_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRM90845.1"
                     /translation="MARIKYSTEEVDLLARLIKSEALGEGEQGMLLVGNVVINRVVAN
                     CDVFRNTRTITEVVYQKNAFSGVGTPLFNEPVNELLRQIALRCINGYRNEPATNALWF
                     KNPGTNVACPEKFYGNLSGRFKQHCFYNPGLKLNCDL"
     gene            2440..>2670
                     /locus_tag="PLK68_10355"
     CDS             2440..>2670
                     /locus_tag="PLK68_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein GerQ"
                     /protein_id="HRM90846.1"
                     /translation="MDYFNDLNPYLVRQDPAPAETPDTTPTPAPAPTPNQTPGTPSTP
                     SVTPAPVIDQAYVENILRINVGKLGTFYFTYTG"
BASE COUNT          837 a          556 c          358 g          919 t
ORIGIN      
        1 gcgcctgctt cattactttt aataaagttt ttgtattaat tacataagtt tctaatgaaa
       61 tactacgatc tttacgacta cccttattac gttctacagc cgttacaact ttatctttga
      121 ttgttaagca atcacatcca atatatgctt cattagcatc atgaactttc ttatatacca
      181 ttgtaatttc agcaccggtt ttttcgtgat tttcaattac atcttcataa tccattgttg
      241 ttatgatatg tgctggagca atcacaacat aatcagcctt attagcttct aaaaaagcga
      301 tattttcaat taaattatta atatcatgat tataattagg gttattggcg tatttttcat
      361 tatataataa tgaaacccca ccagctttag agttaaagtt ccaagaatca ccattaccta
      421 agtgtttaaa taatgatctt ggtttttttt gaatcaatac tccgactgca tcaattgttg
      481 aatttgacat attagataat acgaaatcaa tcaatgcata acgtcctaaa aaactaacag
      541 acgctactgg tcttctctct gtaagaccga caaaatctac atctgagtgt aaattaacta
      601 taccaattgc ttttaccatc tctatttcct cccctactta ttatcactaa ccaaagcgac
      661 ttctttagct tctttgttga tcttagtttt tgcacgaata actacacctt catcaacaat
      721 tgctttatac acttcagcgc cttcttcaac taatacccct ggcattaata ctgaatcaat
      781 aactttagcc ccttcaccaa cattaacgtt gttgaataaa actgatcctt caacattacc
      841 attaactaaa catccttgtg taatcaaaga atttttaact ttagcttgat ccccgataat
      901 ttgtggtttt cctaaagtat cttcagtata gatcttccaa tctttcttga tgttatataa
      961 atcaagatct ttatctttta acaagtccat gttagcctgc cataagcttt caacagttcc
     1021 aacatctttc caatatccat caaattcata agcatataat ttcttgttat catttaacat
     1081 gtcaggaata atgttcattc caaaatcatg tttactatct tcttttttgg catctgcaat
     1141 taaatatttt cttaattgct tataagtaaa gatataaatc cccattgatg ctaatgtaga
     1201 ttttggtttt tctggttttt catcgaattc ataaatagtt ccatcttcat aagtattcat
     1261 gattccaaaa cggcttgctt cttttaatga tacatttaat acagcgatag tggcatcagc
     1321 tttacgttgt ttatgagctg taagcatttt atcataatcc attttataaa tatggtcccc
     1381 agataaaatc aaaacatatt ctggatcata ttgatcgata aagctaatgt tttgataaat
     1441 agcatcagcc gttccagcat accaggttgc tcctacttca ccacgttcac gagccggtaa
     1501 aattgcagct agtgattgtt ttccatcaag tccccattta gttccggcac caacataagt
     1561 acctaacaat acagattcat attgcgtaag caccccaaca atatcaatcc ccgaattagc
     1621 gcagttactt aaaggaaagt caataatacg atatttacct ccaaagtgta ctgctggttt
     1681 agctacttta gctgttaggg cttctagacg tgttccacgt ccaccagcta aaatcattgc
     1741 gacaatctct cttcccattt ttaaatacct ccttgtctaa tgtaagcgct ttacctataa
     1801 tttaattcta ccataaatta gaaataaatt cgagattaat tatacgaaat ataattattg
     1861 tatcttgtca tatattatac tctcatttat ttttttaata aaatgatatc ataattttta
     1921 tccccgcaat tttccctaaa ttcaaacaac tatcattttt tataaataac tgcatatatt
     1981 ataacggggt gataatttgg cacgaattaa atactcaacc gaagaagtcg atttactagc
     2041 gcggcttatt aaatctgaag ctttaggtga aggagaacaa ggtatgctat tagttggtaa
     2101 tgtagttatc aatcgcgttg ttgcaaactg tgatgttttt cgaaacaccc gaaccatcac
     2161 tgaagtagtc taccaaaaga atgctttttc cggggtcgga accccactct ttaacgaacc
     2221 agtcaacgaa ctgcttcggc aaattgcatt acgctgtatt aacggctatc gaaatgaacc
     2281 ggcaactaat gccctatggt ttaaaaatcc cggtaccaat gtcgcttgcc cagaaaaatt
     2341 ctatggcaat ttatccggtc gctttaaaca acactgtttt tataacccag gcttaaaatt
     2401 aaattgtgat ttataaaaaa ttatacgagg agatcgatta tggattattt taatgactta
     2461 aacccttatt tagtacggca ggatccagct ccagctgaaa cccctgatac taccccaacg
     2521 ccagctccgg cccctacccc taaccaaacc ccaggaacac cttctacacc ttcagtaact
     2581 ccagcaccag ttattgatca agcatatgtt gaaaatattt tacggatcaa cgttgggaaa
     2641 ttaggtacct tttactttac gtataccggc
//
LOCUS       DAOMTA010000396         2668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1693_length_2668_cov_2.088787, whole genome shotgun sequence.
ACCESSION   DAOMTA010000396 DAOMTA010000000
VERSION     DAOMTA010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1693_length_2668_cov_2.088787"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLK68_10360"
     CDS             complement(<1..181)
                     /locus_tag="PLK68_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRM90847.1"
                     /translation="MKELENKIIEFVQKRGWDQLEHPECLIKSISIEAGELLECIQWD
                     NDYKTENISEELADVM"
     misc_binding    364..593
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            631..1635
                     /locus_tag="PLK68_10365"
     CDS             631..1635
                     /locus_tag="PLK68_10365"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRM90848.1"
                     /translation="MIEIKRVSKVYRLKDREVVAVDDVSLTIEDGDIYGIIGYSGAGK
                     STLVRLINQLEVQDSGKIFIDGQDLGNLSQIELRKLRTKVGMIFQHFNLLWSRTVSEN
                     IELALEIAGNKDKRIRQQKVQELVELVGLTGRENAYPSELSGGQKQRVGIARALANDP
                     RILLCDEATSALDPDTTKSILELLLKINKKLNITIIMITHQMEVVQRICNHIAVMSEG
                     KVIEEGTVKEIFTSPQHSVTKSFIQEGKNHNEFDETVLKKIYSKGRLLKVVFDENVSR
                     LPILTKVIRECDSDINVIEANLSNTIDSSFGIMILQVIGDYEKVITLFEKYLAKVEVM
                     "
     gene            1636..2301
                     /locus_tag="PLK68_10370"
     CDS             1636..2301
                     /locus_tag="PLK68_10370"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HRM90849.1"
                     /translation="MRSILENIDINAMIAALNETLFMTLISLIFAVILGMVLGIAIYL
                     TQDDGLYPNLIVNKILNLIVNVFRAVPYIILVFLLIPLTTFLVGSMLGAKAALPSLIL
                     SSAPFYGRMVMIALNEVDGGTIEASKAMGASNVQIITKVLIPEAKPALISSITVMSIS
                     LVGYTAMAGAIGAGGLGNLAYLYGFVRTNNYVMYTATFLILVIVFIIQFIGDYFVRKI
                     DKR"
     gene            2311..>2668
                     /locus_tag="PLK68_10375"
     CDS             2311..>2668
                     /locus_tag="PLK68_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM90850.1"
                     /translation="MKAVTLTACGGKTERDDKTLLVAATSKPHGDILEEAKNILKDDY
                     DIDLEIKILDDYYIFNRSLNDSDVDANFFQHVPFFEKDIKDNKYDLVNAGGVHIEPFG
                     FYSKQIKNINELKKNDI"
BASE COUNT          935 a          308 c          529 g          896 t
ORIGIN      
        1 tcattacatc ggcaagttct tcactaatat tttctgtttt gtaatcatta tcccactgaa
       61 tacattcaag taattcccca gcttcaatag aaatactttt aattagacat tcgggatgtt
      121 ctaattgatc ccaaccacgt ttttgaacaa attcaataat tttattttca agctctttca
      181 taaacatctc cttttttata attatatgaa ctattgatag ataatcaagt aaaatgaata
      241 aatgtcgata caaacgggaa taaatttaga tataaaaaag acaggaaaat aatgcatagt
      301 aaaaaagtag gaaataatta aaaaatactc ttgactttta aaatagattt agttaatata
      361 ttgccaacga caatgaaaag aagagtagtt cacttgaact ttatagagaa tccttggatg
      421 gtggaaaagg ataggggata atgaatgaag atggtcttgg agtcttgtag gcgatattta
      481 gcctatgacg ggtgtgcccg ttagcgcact agagtatgat tgtactcgaa gaggttattg
      541 ctgtgaggta ataatgaatt aaggtggtaa cacgggcaat gctcgtcctt tggcttaaag
      601 gacgagtttt tatattttaa ggaggatttt atgattgaaa ttaagcgcgt tagtaaagtt
      661 tatcgattaa aggatcgtga agtagttgct gtagatgatg tttctttaac tatcgaagat
      721 ggcgatattt atggaataat tggttatagc ggagctggta aatctacatt agtgcgtcta
      781 attaaccaat tagaggtaca agatagtggt aaaatattta ttgatggaca agatttaggg
      841 aatttatcac aaatagaatt aagaaaattg agaactaaag taggaatgat ttttcagcat
      901 tttaatttat tgtggtcacg aacagtaagt gaaaatattg aattagcttt agaaattgct
      961 ggtaataaag ataaaaggat tcgtcagcaa aaggttcaag aactagtaga gctagttggt
     1021 ttaacaggtc gtgagaatgc ttatcctagc gaattatcag gtggacaaaa gcaacgggtg
     1081 gggatcgcgc gagctcttgc taatgatcct agaatcttat tatgcgatga ggctactagt
     1141 gcattagatc ccgatacgac aaaatcgatt ttagaattat tattaaaaat aaataagaaa
     1201 ttaaatatta cgatcattat gatcacacat caaatggaag tagtccaaag gatctgtaat
     1261 catattgcag ttatgtcaga aggaaaagtt atcgaagaag gaactgttaa agagattttt
     1321 acttcgccgc aacattcagt aactaaaagt tttattcaag aaggaaaaaa tcataatgaa
     1381 tttgatgaga ctgttctaaa gaaaatttat tctaaaggac gattattgaa agtagtgttt
     1441 gatgaaaatg ttagtcgttt gccaattttg actaaagtaa ttagagaatg tgatagtgat
     1501 ataaatgtaa ttgaagctaa tttaagcaat actatcgata gttcatttgg gattatgatt
     1561 ttgcaagtaa tcggtgatta tgaaaaagta ataactttat ttgaaaaata tttagctaaa
     1621 gtggaggtga tgtagatgcg aagtatttta gaaaacattg atatcaatgc gatgatagca
     1681 gctctaaatg aaacgctctt tatgacattg atttcattga tttttgcggt aattcttgga
     1741 atggtcttgg gaattgctat atatttaact caagatgatg gactgtatcc taatttgatc
     1801 gttaataaaa ttttaaattt gatcgttaat gtatttagag cggtaccata tattatttta
     1861 gtatttttgc taattccatt gactacattt ttggttggtt caatgttagg tgctaaggca
     1921 gcattaccgt cattgatttt atcaagtgca ccattttatg gacggatggt catgattgct
     1981 ttaaatgaag ttgatggtgg aacgattgaa gcaagtaaag caatgggggc aagtaatgtt
     2041 caaatcatta ctaaggtttt aattccagaa gctaaaccag ctttgatttc ttcgattaca
     2101 gttatgtcca tttcattagt tggttataca gcaatggcag gtgccatagg agctggtggt
     2161 cttgggaatc tggcatatct atatgggttt gtaagaacta acaattatgt aatgtataca
     2221 gccacatttt taattttagt tattgttttt attattcaat ttattggtga ttattttgta
     2281 aggaaaatag ataaaaggta gggaaatatt atgaaagcag ttacactaac tgcatgtggt
     2341 ggaaaaacag aacgtgatga taaaacatta ttagtggctg caacctcaaa gccacatggt
     2401 gatattttgg aagaagctaa gaacatttta aaggatgatt atgatattga tctagagata
     2461 aaaattttag atgactatta tatttttaat cgctctttaa atgatagtga tgtagatgca
     2521 aactttttcc aacatgtccc attttttgaa aaggatatta aggataataa atatgatcta
     2581 gttaatgctg gtggagtgca tattgaacca tttggttttt attcaaaaca aattaaaaat
     2641 attaatgaat taaagaaaaa tgatattg
//
LOCUS       DAOMTA010000397         2661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1700_length_2661_cov_1.864543, whole genome shotgun sequence.
ACCESSION   DAOMTA010000397 DAOMTA010000000
VERSION     DAOMTA010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_2661_cov_1.864543"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLK68_10380"
     CDS             complement(<1..294)
                     /locus_tag="PLK68_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HRM90851.1"
                     /translation="MYSYLIGTVVEMNIDHIVVENSGIGYLIYVNNPYEYTRGKEIKI
                     FLYQQVKEDALLLYGFKTSEEKEMFLKLILVKGIGCKTAVGILATGDVTSIISA"
     gene            complement(421..1757)
                     /gene="cgtA"
                     /locus_tag="PLK68_10385"
                     /pseudo
     CDS             complement(421..1757)
                     /gene="cgtA"
                     /locus_tag="PLK68_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
     gene            complement(1844..2128)
                     /gene="rpmA"
                     /locus_tag="PLK68_10390"
     CDS             complement(1844..2128)
                     /gene="rpmA"
                     /locus_tag="PLK68_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRM90852.1"
                     /translation="MMFKLDLQLFASKKGVGSTKNGRDSKSKRLGAKLSDGQFCTAGS
                     IIYRQRGTKVHPGTNVGKGGDDTLFATVDGVVKFERLGRDRKQVSVYPAA"
     gene            complement(2132..2455)
                     /locus_tag="PLK68_10395"
     CDS             complement(2132..2455)
                     /locus_tag="PLK68_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRM90853.1"
                     /translation="MIKVLVKQNNNQIVNLSITGHADSGEYGKDLVCAGVSTVGIGAM
                     NMLAKKGFLAKGLGTIEINEGYINVVVNHTDEVCQVVLETLVVTLETMVESYGRFIKI
                     SKVEV"
     gene            complement(2460..>2661)
                     /gene="rplU"
                     /locus_tag="PLK68_10400"
     CDS             complement(2460..>2661)
                     /gene="rplU"
                     /locus_tag="PLK68_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRM90854.1"
                     /translation="GAPYVKGATVEATVEKQGKEKKVVIYKYNAKKHYHKKQGHRQPY
                     TKLVINTINKTAKKAAEETAEA"
BASE COUNT          819 a          620 c          323 g          899 t
ORIGIN      
        1 tgcacttata atgcttgtta catcaccagt agctaaaatt cctaccgctg ttttacaacc
       61 aatccctttg accagaatta attttaaaaa catttctttt tcttcacttg ttttaaatcc
      121 atataataat aatgcatctt ctttaacttg ttggtaaaga aaaatcttta tttctttacc
      181 tcgtgtatat tcatatggat tattgacata tatcaaataa ccaattccac tattttctac
      241 aacaatatga tcaatattca tctcaactac cgtaccaatt aagtaactat acattcccta
      301 ctccttcaca tttactatta tacagcacat cgtactgttt tttatctcaa aatcgtttca
      361 aaccattctc aacaattata gcattgtaaa agaaaaaaac caactagtat tactagctgg
      421 ttactcataa aaatcaaatt caaactcacc gatagcaaca ttatcaccat cttgagctcc
      481 ggcatttcta agtgcatcat caacacccat attacgcatt ttaatcaata gtcgttttaa
      541 tgaatcatca cttactaacg atgtcattgc caccattctc tcgatcttct tgccagtaat
      601 ttgccaatta ccattaccta aattatgtaa ttcaaatggt ttatcttcct catcacccat
      661 tgtataaaga acagtatgtt caacttcatc ttccattgtg aaagttggtg cagtagctaa
      721 cgcatcagca accgcatata aaacctgatc tacaccatca tgaataatag cactaattgg
      781 gaaaatctta acatcttcac caacctgttt tttaaaacga actaaatttt cttcagcccc
      841 ttcttcatcc attttatttg ctacaacgat ttgaggacgt tctaataaac gatattgata
      901 ttcaccaagt tctttattaa tagtaacata atcctcatat ggatcacggc catcgactgc
      961 tcccatatca acaatatgaa cgattactcg acaacgttca atatggcgca agaacttcag
     1021 tcccagccct tttccctcgg atgctccgcc gataatccca gggatatctg ccatgataaa
     1081 cctttacctt gagccgctcc ctcaatcaat cctggtaaat cagccataac aaaactacgt
     1141 ccatctttta cttgaaccac tcctaaatta ggaacgatcg tagtaaaatg ataatctgca
     1201 atttccgggc gtgctcgtga aacaactgat aagagtgttg atttacctac tgatggaaaa
     1261 ccaaccaatc ccacatccgc caataatttc aattcacaaa taagatcgaa attttcacca
     1321 ggttcaccac gctcacaaat ttgtggtgca ggattccgtg aagttgcaaa acgcgcattt
     1381 ccgcgaccac cacggccacc tttagcaatt acaacacgtt gcttatcttc ggttaaatcc
     1441 gccatgatct gcccagtttc ttcattaaga atcattgttc caaccggtac tttaatgacc
     1501 gtatcaatag catctttacc atgcattttt ttagccatcc cattttgacc agatggtgcc
     1561 ttatataatc gattatattt caaatctaac aaagttgata atgaggtcgt cgcttgaaaa
     1621 atcacacttc caccacgacc gccatcacct ccagctggac caccttttgg tacatgggcc
     1681 tcacgtctaa aagctactgt cccgtcaccg ccttttccag cttcaactct aatttttgct
     1741 ttatcaataa actgcatata tccacctctt ttcatctctt tattattatg atacaaataa
     1801 tcagttatta gtacaagaaa aaatcccttt gcagggattt taattatgca gctgggtaaa
     1861 cagaaacttg ttttctatct ctaccaagtc tttcgaattt tacaactcca tcaactgtag
     1921 caaataaagt gtcatctcca ccttttccta cattagtacc tggatgaact ttagttcctc
     1981 tttgtctata aataatagac cctgcagtac aaaattgccc gtcagataat ttagctccta
     2041 atctttttga tttagaatca cgaccatttt tagttgaccc tacccctttt ttagaagcga
     2101 ataattgtaa atctaatttg aacatcatat gttacacctc cactttagat atcttaataa
     2161 atcgaccata gctttcaacc atcgtttcta aagttacaac aagtgtctct agcaccactt
     2221 gacaaacttc atcagtatga ttgacaacta catttatata tccttcatta atctcaatcg
     2281 ttcctaagcc tttagctaag aatccctttt ttgcaagcat attcattgct ccaataccaa
     2341 cagtagaaac accagcacaa actaaatctt taccatattc tccactgtca gcgtgacctg
     2401 taatactaag attaacaatt tgattattat tttgttttac taaaacctta atcatttgat
     2461 taagcttctg cagtttcttc agcagctttt ttagcagttt tattaattgt attgattact
     2521 aattttgtat aaggttgtct atgaccttgt tttttgtggt aatgtttttt agcgttatat
     2581 ttataaataa caactttctt ttctttacct tgtttttcaa cagtagcttc aacagtagca
     2641 ccttttacat aaggagcacc t
//
LOCUS       DAOMTA010000398         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1702_length_2660_cov_2.193858, whole genome shotgun sequence.
ACCESSION   DAOMTA010000398 DAOMTA010000000
VERSION     DAOMTA010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_2660_cov_2.193858"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLK68_10405"
     CDS             complement(<1..243)
                     /locus_tag="PLK68_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRM90855.1"
                     /translation="MNKNRIDAVVNNMKEAGLDYLLISEPSSIDYLIDYVNNPGERMY
                     VLMLAANGAHKLFFNKLFFVENDLGIEIVWHSDTDDA"
     gene            complement(230..1987)
                     /gene="aspS"
                     /locus_tag="PLK68_10410"
     CDS             complement(230..1987)
                     /gene="aspS"
                     /locus_tag="PLK68_10410"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM90856.1"
                     /translation="MNRTHNNGELRISDVNKIVELKGWVAKKRNLGSLVFIDLRDRYG
                     ITQIVLNEEMEALGKDIKNEYVLHVVGKVIERSSKNPNMPTGDIEIEAAQIDIINTAK
                     VTPIYTTDDTDASEDARMQYRYLDLRRPVMQNKIIMRHKITSSIRNFLDNHEFIDIET
                     PYLNRSTPEGARDFLVPSRVHNGSFYALPQSPQLFKQLLMVSGFERYYQIARCFRDED
                     LRLDRQPEFTQVDVEMSFMTTDEIISIGEQLLAQVMKDVKGIELQLPLPRMTWHEAME
                     KYGIDKPDIRFGLELVNLNEVVKDVEFMVFKSALEADGHVKGINVKGEADNFSRKAID
                     KLTDVVKTYKAKGLAWLKVKAGKAEGPIAKFFNEEQMTKLLKAMDASDNDLLLFVSDA
                     KYNVVCDALAALRNHLGKELKLFNPDEFAFLWVVDFPMFEYDDETERYYAVHHPFTRP
                     KDSDIDKIENDPANCLADAYDIVLNGFELGGGSQRIYEQELQERAFRALGFTQERIDS
                     QFGWFVEAFQYGTPPHGGFALGLDRLAMLLTGSENIREVIAFPKNASAVCPMSKAPSE
                     VDNAQLEELGIAVIEDEQE"
     gene            complement(1980..>2660)
                     /locus_tag="PLK68_10415"
     CDS             complement(1980..>2660)
                     /locus_tag="PLK68_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRM90857.1"
                     /translation="EANPLRILDCKVDHNHPAMANAPKTVDYLSEAAKAHFTKVCELL
                     DDLEIEYVVDTNLVRGLDYYCHTVFEVISDDPRLGAGATVGGGGRYNGLVQELGGPEA
                     PGVGFAFGMERLLIAMDEELEEPEGLDVYVMPMGDEARDLAVQITAMLRANGFSVDMD
                     YQGRSLKAQFKTVDRLNSHFAMIIGDQEIADEVVNIKCTHSKTQDIVPIENIVAYIEN
                     HMEGHVHE"
BASE COUNT          842 a          586 c          335 g          897 t
ORIGIN      
        1 cgcatcatca gtatcgctat gccaaacaat ttcaatccct agatcatttt ctacaaagaa
       61 taatttattg aagaacaact tatgggcacc atttgctgca agcatcaata catacattct
      121 ttcaccgggg ttatttacat aatcaatcaa ataatcaatt gaagaaggtt cactgatcaa
      181 caaataatca agaccagctt ccttcatatt atttacaact gcatcaattc tattcttgtt
      241 catcttcaat caccgcgatt cctaattctt ctaattgagc attatctacc tcacttggtg
      301 cttttgacat tggacatact gcacttgcat tttttggaaa agcaataacc tcacgaatat
      361 tttcactacc agtaagtaac attgctagac gatccagacc taaggcaaat cccccatgtg
      421 gtggagtacc gtattgaaag gcctcaacaa accagccaaa ttgactatca attctctctt
      481 gggtaaaacc taatgctcta aaggctcttt cttgtaattc ttgttcgtag atacgttgtg
      541 aacctccacc caattcaaaa ccgtttaata caatgtcata agcatcagct aaacaatttg
      601 caggatcgtt ttcaatttta tcaatatcac tatctttagg tcgagtaaat ggatgatgaa
      661 ccgcataata acgttcagtt tcatcatcat attcaaacat tggaaaatct acgacccata
      721 aaaatgcaaa ttcatcaggg ttaaataatt ttaattcttt tcctaaatga ttacgtaaag
      781 ctgccaaagc atcacaaaca acattatatt tagcatcgct aacaaataat aataagtcat
      841 tatcacttgc atccattgct tttaataatt ttgtcatttg ttcttcgtta aagaatttag
      901 caattggtcc ctcagccttt ccagctttaa cttttaacca tgctaatcct tttgctttat
      961 atgttttaac tacatccgtc aatttatcaa ttgctttacg agaaaaattg tcagcttcac
     1021 ctttgacatt aattcctttt acatgcccat cagcttctaa cgcgctctta aaaaccataa
     1081 actcaacatc tttaaccact tcatttaaat taactaactc taatccaaaa cgaatatctg
     1141 gcttatcaat tccatatttt tccatagctt catgccaagt cattcgtggc aatggcagtt
     1201 gcagttcaat tcctttaaca tctttcatta cttgtgctaa cagctgttca ccaatactaa
     1261 taatttcatc agtagtcatg aaactcattt caacatcaac ttgagtaaat tccggttgcc
     1321 gatctaatcg taaatcctca tctctaaaac agcgtgcgat ttgataataa cgttcaaatc
     1381 ctgaaaccat caataattgc ttaaataatt gtggtgactg tggtaaagca taaaatgaac
     1441 cattatgaac ccgtgaaggg actaagaaat ctcttgctcc ctcaggagta gaacgattta
     1501 aatagggcgt ttcaatatca ataaattcat gattatctaa gaaatttcga atactgctag
     1561 taattttatg acgcataatt attttattct gcattaccgg tcttcttaaa tcaagataac
     1621 gatattgcat tcttgcatcc tcacttgcat cagtatcatc agttgtgtaa ataggtgtta
     1681 ctttagccgt attgataata tcaatttgag cagcttcaat ttcaatatcc ccagttggca
     1741 tattaggatt tttagaactt ctttcaatta ctttaccaac tacatgcagg acatattcat
     1801 ttttaatatc cttacctaga gcttccattt cttcatttaa tacgatttgc gtgattccat
     1861 aacggtctct taaatcaatg aaaaccaaag aacctaaatt tcttttttta gcaacccagc
     1921 cttttaattc aacaatttta ttaacatcac taattcttaa ttcaccattg ttatgagttc
     1981 tattcatgta catgtccctc catatgattt tcaatatacg caacaatatt ttcaattggt
     2041 acaatatctt gtgtctttga atgtgtacat ttaatattga ctacttcgtc agcaatttct
     2101 tgatcaccaa tgatcattgc aaaatgacta ttcaaacgat caaccgtttt aaactgtgct
     2161 tttaaagaac gaccttgata gtccatatca acactaaagc catttgctcg tagcattgcc
     2221 gtgatctgta ccgctaaatc tcttgcttca tctcccattg gcataacata cacatccaac
     2281 ccctcaggtt cttctaattc ttcatccata gcaatcaata atcgttccat tccaaaagca
     2341 aatccaactc caggtgcttc tggtccccct aattcctgta ctaagccatt ataacgacca
     2401 ccgccaccaa cagtggctcc tgcacctaag cgtggatcat cagatattac ttcaaaaact
     2461 gtatgacagt aataatctaa accacgcact aaatttgtat caacaacata ttcaatttct
     2521 agatcatcta ataactcaca tactttggta aaatgtgcct tagccgcttc acttaaataa
     2581 tcaactgttt ttggcgcatt tgccatcgct ggatgattat gatctacttt acaatctaaa
     2641 attcttaacg gatttgcttc
//
LOCUS       DAOMTA010000399         2659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1704_length_2659_cov_2.143241, whole genome shotgun sequence.
ACCESSION   DAOMTA010000399 DAOMTA010000000
VERSION     DAOMTA010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1704_length_2659_cov_2.143241"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLK68_10420"
     CDS             <1..426
                     /locus_tag="PLK68_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM90858.1"
                     /translation="LRGLRTLPVRLKSQQETLQQVINFLQEDPRIERIYHPLCNGEQQ
                     SELAHKYLKGYGSLLGVVLKDANPEIIKRFIDSLEHFTLAYSWGGFESLVMAVYKGNN
                     INEIKERGLSLGQLRMYIGLEDSELLISDLKNALDQAYQ"
     gene            complement(465..1028)
                     /locus_tag="PLK68_10425"
     CDS             complement(465..1028)
                     /locus_tag="PLK68_10425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90859.1"
                     /translation="MEEITVYSLDYYQMSKEIKREIVHLLHVVWPSDDCNDAHNEELT
                     VQSFYIMKDNQVVSYGAVLRMETTIDNEKYQIGGLSCIATLPNYRRQGLSSKIVQTAT
                     KWIEDNLDFGIFTCKRELEDFYACNGKWQVANNVVLIANRDNNALSSNKLAVIVLIRL
                     FSNKAQVNGQKILNARIYLDLPQGQFI"
     gene            complement(1028..1669)
                     /locus_tag="PLK68_10430"
     CDS             complement(1028..1669)
                     /locus_tag="PLK68_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM90860.1"
                     /translation="MNKKYILFDLDGTLTDPMKGITKSVRYALNYYGIEVNDLNDLLP
                     FIGPPLRDSFQEFYGFDALKAEEAVVKYREYFSTKGIFDNKVYPGIEVCLQTLKDQGK
                     VLLVATSKPEKFAKEIIEHFGLAKYFDFVGGSEFNGREKKAEVIDYVLTANMIDKDEA
                     IMVGDRKHDVIGAHENDLPCIGVLYGYGTKEELMACNSDYLVADINALQELLG"
     gene            complement(1699..>2659)
                     /locus_tag="PLK68_10435"
     CDS             complement(1699..>2659)
                     /locus_tag="PLK68_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90861.1"
                     /translation="ALEFSSINKNTPTLKLDYGHWRAENEFFGIIDEDYNFLKVFTLE
                     NEQIRECPINEYYGSIAGHEFSFKYADYQERIVAFDFNGCIYSVLPKIINNDIYVCVD
                     INDGEQRALNIAKINLESKQISFITNDNISVNPITSPQQTNILINKSDQGWENYNIET
                     GETNLIKNIDPYMHSNCITFIDEQTVVTYDENGHSYLINILTNEVRTLDKYPLEGTIV
                     NIEYSDETIKLTNIITGAVSRIDYHFYGTNYMGTYCSIDYLVLFNNEKLYLYDIESNQ
                     LVDLDPQKELDEQLKEVMIIDQKHLLITTDKRVYIVANVGNNG"
BASE COUNT          930 a          489 c          309 g          931 t
ORIGIN      
        1 cttcgtggtc taagaacatt accggttcgt ctaaaatcac aacaagaaac acttcaacaa
       61 gtaattaatt ttctacaaga ggatcctcga atcgaacgaa tttatcatcc tctttgtaac
      121 ggagagcaac aaagcgaact tgctcataaa tatcttaaag gatatggtag tttattaggg
      181 gttgtcttaa aagatgcaaa tccagaaatc attaaacgtt ttatcgattc attagaacat
      241 tttactttag cctatagttg gggtggtttt gaaagtttag taatggcagt ttataaagga
      301 aacaatatta atgagataaa agaacgtggt ctatctttag gacaacttcg catgtatatc
      361 ggacttgaag atagtgaatt attgattagt gatttaaaaa atgccttgga tcaagcatat
      421 cagtaataaa aataagggtt gagcaatatt actcaaccct ttctttaaat aaattgacct
      481 tgaggcaaat ccagataaat tctcgcattt aaaatttttt gtccattaac ttgagcttta
      541 tttgaaaaaa gacgtattag tacaataact gcaagtttat tactagatag agcattatta
      601 tcacgattag caattaaaac aacattattt gccacctgcc atttgccatt acaagcataa
      661 aaatcttcaa gctctcgttt acacgtaaaa attccaaaat ctaaattatc ctcaatccat
      721 ttagtcgctg tttgaactat ttttgaactt aatccctgcc tacgataatt tggcaaggta
      781 gcaatacaac tcaatccacc aatttgatat ttttcattat ctatcgttgt ttccatccga
      841 agaactgctc cgtaactaac aacctgatta tctttcatga tatagaatga ctgcactgta
      901 agttcttcgt tatgggcatc attacaatca tcactaggcc aaacaacatg taaaagatgt
      961 acaatttctc tttttatttc tttggacatt tgataatagt ccaaagaata aacagtaatt
     1021 tcttccatta tccaagtaat tcttgcaatg cattaatgtc cgcaactaaa taatcactat
     1081 tacaagccat caattcttct tttgtcccat aaccatacaa cactccgata caaggtaaat
     1141 cattttcatg ggctccaatc acatcatgct ttcgatcccc aaccataatc gcttcatctt
     1201 tatcaatcat atttgctgtt aaaacataat caattacttc agcctttttt tctcgtccat
     1261 taaactcact accgccaaca aaatcaaaat acttagctaa accaaaatgt tctataattt
     1321 ctttagcaaa tttttctggc tttgaagtag caactaacaa tacttttccc tgatctttca
     1381 aagtttgcaa acacacttca attcccggat atactttatt atcaaaaatt cccttggttg
     1441 aaaaatattc gcgatattta actactgcct cttcggcttt taaagcatca aaaccataaa
     1501 attcttgaaa agaatctctt aaaggtggtc caataaaagg taacaaatca tttaagtcat
     1561 taacttcgat tccataataa tttaaagcat aacgtactga tttagtaatc cccttcatcg
     1621 gatcagttaa ggttccatct aaatcaaaca aaatatattt tttattcata taagtcctcc
     1681 actacactaa cttatatttt atccattatt ccctacatta gcaactatat ataccctttt
     1741 atctgtagta attaataaat gtttttgatc aatgatcatt acttctttta attgctcatc
     1801 taattctttt tgtggatcca aatcaactaa ctgattactt tcaatatcat aaagataaag
     1861 tttttcatta ttaaataaaa caagataatc tatgctacaa taagttccca tataattagt
     1921 accataaaaa tgataatcaa ttcttgaaac agcgcctgta ataatatttg ttaacttaat
     1981 cgtttcatca ctatattcaa tattgacaat cgttccttcc aaaggatatt tatctaacgt
     2041 tctaacttca tttgttaaaa tattaattaa ataagaatgt ccattttcat cataagtaac
     2101 aacagtctgt tcatcaataa aagtaataca attactatgc atatatggat cgatattttt
     2161 aatgagattg gtttctccag tttcaatatt ataattttcc cacccctgat cactcttatt
     2221 aattaaaata ttagtttgtt gtggtgaagt aattggattt actgaaatat tatcattagt
     2281 aataaaactt atttgcttac tttctaaatt aatcttagca atatttaaag cacgctgctc
     2341 tccatcatta atatcgacac aaacataaat atcattatta ataattttag gcaaaacact
     2401 atatatacat ccattaaaat caaatgcaac tattcgctct tgataatccg catatttaaa
     2461 actgaattca tgaccagcta tactcccgta atactcatta ataggacatt ctcttatttg
     2521 ttcattttct aacgtaaaaa ctttaagaaa attataatcc tcatcaatga ttccaaaaaa
     2581 ttcattttca gcacgccaat gcccataatc tagttttaat gtgggagtat ttttgttgat
     2641 tgaagaaaat tcaagagct
//
LOCUS       DAOMTA010000400         2657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1706_length_2657_cov_2.031130, whole genome shotgun sequence.
ACCESSION   DAOMTA010000400 DAOMTA010000000
VERSION     DAOMTA010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_2657_cov_2.031130"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLK68_10440"
     CDS             complement(<1..218)
                     /locus_tag="PLK68_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM90862.1"
                     /translation="MWIGLACLSALFAGVTAVLAKIGLKNVDSSLATALRTIIVLIFS
                     WIMVLFAGTQEQLKYIDSNTVLVLCFSG"
     assembly_gap    364..393
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<394..1028)
                     /locus_tag="PLK68_10445"
     CDS             complement(<394..1028)
                     /locus_tag="PLK68_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM90863.1"
                     /translation="MNKPFVLVVEDDNAVRNLIATTLETHDYKYHTSQTGKQAIIETA
                     SQKPDIMLLDLGLPDLDGVEIIKKVRTWTNMPIIVISARSEDRDKIEALDAGADDYLT
                     KPFSVEELLARLRVILRRLNYNQSQNIESSDFVNGDLKIDYAGGCAYLDQQELHLTPI
                     EYKLLCLLSRNVGKVLTHTYITKEIWGSTLESDVASLRVFMATLRKKIELDP"
     gene            complement(1021..>2657)
                     /locus_tag="PLK68_10450"
     CDS             complement(1021..>2657)
                     /locus_tag="PLK68_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM90864.1"
                     /translation="FFGHSQKDRKHLFSSRLDKKLLNLLKDLDINIVYHQNTGKDKSK
                     QRDFNFVVKGKDLAISIFILLIATLIGILFHHLGFSEANTITVYILSVLITAITTSNR
                     IYSLVSSILSVLIFNFMFTNPRFTLAAYGSGYPVTFAIMLIAAFITSTLAIRIKQQAK
                     QSAQHAYRTKVLLETNRLLQKEKSIAGIKETAAKQIVKLLNKSVMIYQITENDTILHE
                     VFHHNNMTVHDCDEIETNVHWVYQNNKTYQAKNYYLPLASTNDIYGVVVIILEDVILD
                     AFENNLVLSIVGECALALEKEFFNQKKEEAAIEAKNERLRANLLRSISHDLRTPLTSI
                     SGNASVLINNSGSLDENKKMQLYEVIYDDSLWLINLVENLLSVTRIEDGKMNLHLETE
                     LIEEVINEALNHISHKKDEHRIEVKANDEFILAKIDAGLIIQVIINIVDNAIKYTPKG
                     SMISIETFKHKDFVEIQIADDGAGISDKDKEKLFEMFYTVKHEVIDGRRGLGLGLALC
                     KAIIVAHGGNIAVKDNIPHGTIFSFTLPIKEVEIHE"
BASE COUNT          894 a          494 c          321 g          918 t
ORIGIN      
        1 attccagaaa aacataaaac caatactgta ttactatcaa tatattttaa ttgttcctgg
       61 gttccagcaa ataagaccat tatccatgaa aaaattaaaa caattattgt ccttaatgct
      121 gttgctaaag aagaatcaac attcttcaaa ccaatttttg caagaactgc agtaacccca
      181 gcaaataaag ctgatagaca agctaatcca atccacaccc taagtttccc cctatactta
      241 ttatatttat attttaccgc aattaacaaa aaagaaaagt gaaacaaact gtttcactca
      301 tattattcaa ttcgtaacat tcggtatcca actccaacat gggtctgaat atatttagga
      361 tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggatcta attctatttt tttacgaagc
      421 gtagccataa atactcttaa tgaggcaaca tcactctcta aagtactccc ccaaatttct
      481 ttagtaatat atgtatgtgt taatactttg ccaacatttc ttgagagaag gcacagcaac
      541 ttatattcaa tcggtgttaa atgcagttct tgctgatcta aatatgcaca tccgccagcg
      601 taatcaattt ttagatcacc attaacaaaa tcactacttt ctatattttg actttgatta
      661 taattaagcc gtcttaaaat aacacgtaat cttgctaaaa gttcctcaac tgaaaaaggt
      721 ttggtcaaat aatcatcagc cccagcatca agagcctcaa ttttatcacg atcttcactt
      781 cgagcactaa taacgattat tggcatattt gtccaagtac gtactttttt tataatttca
      841 accccatcta aatcaggcag ccctagatcc aacagcatta tatccggttt ttgtgaagca
      901 gtttcaataa ttgcttgttt tcccgtttgt gaagtatgat atttataatc atgagtttct
      961 aaagttgtag caatcaaatt tctaactgcg ttatcatctt ctacaactaa tacaaatggc
     1021 ttattcatgt atttcaacct ccttaatcgg caatgtaaaa ctaaaaatcg ttccgtgagg
     1081 aatattgtcc ttaactgcaa tattaccacc atgcgctaca ataatagctt tacacaaagc
     1141 taaccctaaa ccaagtccgc gacgtccgtc aataacctca tgctttacag tataaaacat
     1201 ttcaaaaagt ttttctttgt ccttatcact gattcctgct ccatcatcag caatttgaat
     1261 ttctacaaaa tctttatgct taaaggtttc aatactaatc attgaacctt ttggtgtata
     1321 tttaattgca ttgtcaacaa tatttataat tacttgaatt attaagccag catctatttt
     1381 tgctaaaata aattcatcat tagcctttac ctcaatacgg tgttcatcct ttttatgact
     1441 aatatgattc aatgcctcat ttataacttc ctcaataagt tctgtttcta aatgcagatt
     1501 cattttacca tcttcaattc ttgtaactga aagaagattt tcaaccagat taatcaacca
     1561 taatgaatca tcgtagatta cttcgtataa ctgcattttt ttattttcat ctaagctgcc
     1621 gctattattt atcagtacac ttgcattacc agaaatacta gttaatggtg ttcgtaaatc
     1681 atgcgaaatt gaacgtaata aatttgcacg caaccgctca tttttagctt caattgcagc
     1741 ttcttctttc ttttggttaa aaaactcttt ttctagagct aaagcacatt caccaacgat
     1801 tgatagtact aaattatttt caaaagcatc aagaataaca tcttctaaaa ttattactac
     1861 tacaccataa atatcattag tactagcaag cggtaaatag taattcttag cttgatatgt
     1921 tttattattc tggtagaccc agtgaacatt agtttcaatc tcatcacaat catgaacagt
     1981 catattatta tgatgaaaga cttcatgtaa aatcgtatca ttttcagtaa tttgataaat
     2041 catcactgat ttatttaata atttaacaat ttgtttagct gctgtttctt taattcctgc
     2101 aatacttttt tctttttgca aaagacgatt tgtctctaat agaacttttg tacgatatgc
     2161 atgttgagca gactgttttg cctgctgttt tatccttatt gctaaagtac tcgtaataaa
     2221 agcggctatc agcataattg caaaggttac aggataacca gaaccataag ctgctaaagt
     2281 aaaacgtgga ttagtaaaca taaagttaaa aattaaaaca cttaaaatcg atgatacaag
     2341 actataaata cggttagatg tagttatagc agtaattaaa acacttaaaa tataaactgt
     2401 aattgtattt gcttcactaa aacctaaatg atggaataat atccctatca gggttgctat
     2461 caacagaata aatattgaaa tagctaaatc tttaccctta acaacaaaat taaaatctct
     2521 ttgctttgat ttatctttcc ccgtattttg atgatagaca atattaatat ccaaatcttt
     2581 tagtaaattt aataatttct tatctagcct tgaactaaat aaatgtttcc tgtccttttg
     2641 actatgtcca aaaaaca
//
LOCUS       DAOMTA010000401         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1710_length_2654_cov_2.382840, whole genome shotgun sequence.
ACCESSION   DAOMTA010000401 DAOMTA010000000
VERSION     DAOMTA010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1710_length_2654_cov_2.382840"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1087
                     /locus_tag="PLK68_10455"
     CDS             <1..>1087
                     /locus_tag="PLK68_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRM90865.1"
                     /translation="NIIKAFKENIKFHKLLTLKERISLIGIMGIWIVLENGLIQILPD
                     ALIKNADFNRYFLKNLIISSRFRIYLLEYIIYTLIIMVLMIVFYQYLIGVMGKHEPEL
                     LKVKVDLEFNRLFDRTLLKAQIHGNRFFQSLDKYFFETHFYQKYRGMINLVFWPCMFI
                     LITYLFPNSIYILSAILIVVLLMYFAASIIIRYEKRKNGQEFEDDSGLLFLPYRLIKR
                     TLNNSRLYNHHPRMVTALLAFISVCLVGMYLEQPLMLHHPWVIGHRGSIYGVENTDGA
                     IMTAADKGADYAEIDVQLSKDGVPVVIHDADLSRLAHKDEKVKNMTAKQLSETLVYHN
                     EYTDKIPTLDHLIKKLKKNSTKMGLLIE"
     assembly_gap    1088..1097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1098..1537
                     /locus_tag="PLK68_10460"
     CDS             <1098..1537
                     /locus_tag="PLK68_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRM90866.1"
                     /translation="GNGEKLAKKIIDVIEKNDYQKQAIYMSLDLDTVQYLQSQRPEWW
                     IGYCIYGSAGDIEGSLWNQGIDFLAIEENRATVSFVEKANRNWVPVYVWTVDDESRMI
                     QYLELGVSGIITNYPNRGRKAVDKFKENNYQYYYHHGSGYPDS"
     gene            1644..2240
                     /locus_tag="PLK68_10465"
     CDS             1644..2240
                     /locus_tag="PLK68_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM90867.1"
                     /translation="MKIMIVEDDLILARELQLLCVKWNFQAKYLEDFKHVDQVYCTYQ
                     PDLILLDINLPFYDGFYWCQKIRENSTVPILFISSREQNADKILALSAGGDDYIEKPF
                     DLELLLVKIKAILRRTYEYKHLEKEYLDEDTSYDIVRGRFVYQNKVLELTKSEGKIMS
                     TLLGQRGNWVTREQLMMTLMNLLLMRLYRFIFHVYEIN"
     gene            2216..2305
                     /locus_tag="PLK68_10470"
     CDS             2216..2305
                     /locus_tag="PLK68_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90868.1"
                     /translation="MSRLRNKLKELTNGKDIIKTKKGVGYYID"
     gene            2298..>2654
                     /locus_tag="PLK68_10475"
     CDS             2298..>2654
                     /locus_tag="PLK68_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM90869.1"
                     /translation="MINYLKEKLRPIVGGMLVLVFINIYFLYLCGSRLYLSDLVYLDV
                     ILIFIGITIFIYGNVKYRAVEKIMKTNNFLTTKELKSYLSLQALSVIEQNDLYYHEEI
                     NHLSTQIQELSDYISRW"
BASE COUNT          902 a          292 c          518 g          932 t
ORIGIN      
        1 aatattatta aagcctttaa agaaaatata aagttccata aattattgac attaaaagaa
       61 cgtatttcat taattggaat aatgggtatt tggatcgttc ttgagaatgg attaattcaa
      121 atattaccag atgctttaat aaaaaatgcg gattttaatc gttatttttt aaagaattta
      181 attatttctt cgagatttag aatttattta ttggaataca tcatatatac attaataatc
      241 atggttttga tgattgtttt ctatcaatat ttaataggtg ttatgggtaa gcacgagcct
      301 gaactattga aagtaaaggt ggatttagaa tttaatcgct tatttgaccg aactttatta
      361 aaagcacaaa ttcatggtaa tcgttttttt caaagcttgg ataaatattt ttttgaaaca
      421 catttttatc aaaagtatcg tgggatgatc aaccttgttt tttggccctg tatgtttatc
      481 ttgattactt atctttttcc taattcaata tatattttat cagctatctt aattgttgtt
      541 ttgttgatgt attttgctgc tagtattatt attcgttatg aaaaaagaaa aaatggtcag
      601 gaatttgaag atgatagtgg tttgctgttt ttgccttatc gcttaattaa acgaacttta
      661 aataattctc gcttatataa tcatcatcct agaatggtta cagcactctt agcttttatt
      721 tctgtttgtc tagtaggaat gtatttagaa caaccgttga tgctccatca tccatgggta
      781 atcggtcatc gtggttcaat ttacggggtt gaaaatacag atggagctat catgacagca
      841 gctgataaag gagcggatta tgcagaaatt gatgtgcaat tatctaaaga cggggttcct
      901 gttgtgattc atgatgcaga tttgtcgcga ttggcacata aagatgaaaa agtaaaaaat
      961 atgactgcta aacaattaag tgaaaccttg gtttatcata acgaatacac tgataaaatt
     1021 ccgacgctag atcatttgat taaaaagtta aaaaagaata gcactaaaat gggacttttg
     1081 attgagtnnn nnnnnnngcg gtaatggaga aaaactagct aaaaaaatta ttgatgttat
     1141 agagaaaaat gattatcaaa agcaggctat ctacatgtcg cttgatttag atactgttca
     1201 atatcttcaa agtcagcgtc ctgaatggtg gattggatat tgtatttatg gtagtgctgg
     1261 agatattgaa ggatcattat ggaaccaagg tatcgacttt ttggcgatag aagaaaatcg
     1321 agctacagtg agttttgtag aaaaggctaa tcgtaattgg gttcctgttt atgtatggac
     1381 agtagatgat gaaagtagga tgatacagta tcttgaactt ggggttagtg gaatcattac
     1441 taattatcct aatcggggga gaaaagcagt cgataaattc aaagagaata attatcagta
     1501 ttattatcat catgggtcag gatatcctga tagttaaaac tagattgcga tctagttttt
     1561 tttctaacag ttttgtaagg ttggttaaat tactaggcta tcttaagagg cgatagtata
     1621 atgaataaaa ggagttgaag agaatgaaaa tcatgatagt tgaagatgat ttgatcttgg
     1681 cgcgtgaatt gcaattatta tgtgttaagt ggaattttca ggcaaagtat cttgaggatt
     1741 ttaagcatgt agatcaagta tattgtacat atcagccgga tttgatttta ttagatatta
     1801 atttgccttt ttatgatggt ttttactggt gtcaaaagat aagggaaaat tccacagtac
     1861 caatattatt tatttctagt cgagagcaaa atgctgataa aattctggca ctgtcagctg
     1921 gaggcgatga ttatattgaa aaaccatttg atttagaatt gctgttggtg aaaataaaag
     1981 caatattgag aagaacttat gaatataaac atttggaaaa agaatatctt gatgaggata
     2041 cctcttatga tattgtgagg gggcgatttg tatatcaaaa taaagtttta gaattgacta
     2101 aatcagaagg taagataatg tcgacactat tagggcaacg aggcaactgg gtaaccaggg
     2161 aacagttgat gatgacactg atgaatttgt tactgatgcg actttatcgg ttcatatttc
     2221 acgtctacga aataaattaa aggaattaac gaatggaaaa gatattatta agacgaaaaa
     2281 gggagtaggt tattatattg attaattatt taaaagagaa attacggcct attgtaggtg
     2341 gaatgttagt tttagtattt attaatattt attttttata tttgtgtggt agtcgcttat
     2401 atttaagtga tctggtttat ttggatgtta ttttaatttt tattgggatt acgatcttta
     2461 tttatggaaa tgtgaaatac cgagcagttg aaaaaataat gaaaactaat aattttttga
     2521 ccacaaagga attaaaaagc tatttatcgt tgcaagcact tagtgttatt gagcaaaacg
     2581 atttatatta tcatgaagaa attaatcact taagtactca aatacaagaa ttatcggatt
     2641 atatttctag atgg
//
LOCUS       DAOMTA010000402         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1714_length_2651_cov_2.115948, whole genome shotgun sequence.
ACCESSION   DAOMTA010000402 DAOMTA010000000
VERSION     DAOMTA010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1714_length_2651_cov_2.115948"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1113)
                     /locus_tag="PLK68_10480"
     CDS             complement(46..1113)
                     /locus_tag="PLK68_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HRM90870.1"
                     /translation="MTIYQAMQLNVKNLKAAISDTPTKKDKHRYIAAMILRNIFCLAF
                     CIIFITFFTTLFGNENSSVGIIGLLAVLSLRFTDLDFNIKQSTLALIASFVICAIAPH
                     LANIVPLGFGLLINFSALLLILILTSHQVSYANHFTFILGYILLWGNDVSENSYTLRI
                     IAMMVAAIFTALVYYHCHYQQTMKLNFKDILKDFFSPSKRTFWYLKISSAIALVIFLG
                     EMLNFSRTMWIAFACMSIINIDHEQIIYKFKHRALFAVVGSIIFGILFTIVPKEYLGL
                     AGILGGIMVGFSGSYHWQTVFNCFGALAITIDLFGFLNAIIIRIVANIAGSIFSFVYH
                     HLFEKIIQLLINENLMFDNNL"
     gene            complement(1113..>1439)
                     /locus_tag="PLK68_10485"
     CDS             complement(1113..>1439)
                     /locus_tag="PLK68_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM90871.1"
                     /translation="LDLLSILIIDDEIITPIIISKRMGVSKPLVSRLIEQLNKKELLL
                     KSPHPFDKRSYCLKITAQGRKHLDDIYTYYLEPIYQLKKKLPSDDFTQLINLIKKSNI
                     TLKGGQ"
     assembly_gap    1440..1473
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2092..2412
                     /gene="rnpB"
                     /locus_tag="PLK68_10490"
     ncRNA           2092..2412
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLK68_10490"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2498..>2651
                     /locus_tag="PLK68_10495"
     CDS             2498..>2651
                     /locus_tag="PLK68_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRM90872.1"
                     /translation="MNKHDKTRYVFAQAIKGLIKVHPLDKIAVTDIVTRSGMTRQTFY
                     RYFKDKY"
BASE COUNT          942 a          356 c          453 g          866 t
ORIGIN      
        1 gtttagttgt ttaaactcta agagcacttt taattagata ttctattata aattattatc
       61 aaacattaga ttctcattta ttaataattg aataatcttt tcaaaaaggt ggtgataaac
      121 aaagctgaaa atactaccgg cgatattggc aacgattcta atgatgattg catttaaaaa
      181 tccaaataaa tcaatggtga ttgctaaggc gccaaaacag ttgaaaacgg tctgccaatg
      241 ataactgccg ctaaaaccta ccatgattcc tcctaaaatc cctgctaacc ctaaatattc
      301 tttaggaact attgtaaata gtatcccaaa aattatactg ccaacaacag caaaaagagc
      361 acgatgttta aatttataga taatttgttc atgatcgatg ttgattattg acatacatgc
      421 aaatgcaatc cacatagttc ttgaaaaatt aagcatttct ccaagaaaaa taaccaaggc
      481 aatcgcactg ctgattttta gataccaaaa tgttcgcttg ctaggactaa aaaaatcttt
      541 aagaatgtct ttgaagttaa gtttcatcgt ttgttgatag tgacaatgat aatagacaag
      601 tgctgtaaag atggctgcaa ccatcatggc aataatccgc agagtataac tgttttcgct
      661 gacatcgttt ccccataaga gaatataacc aagaataaag gtaaaatggt tagcgtaact
      721 aacttgatga cttgtaagta ttaagattag tagtaaagcg ctaaaattta ttaataaccc
      781 aaagcccaat gggactatat tggctaaatg cggagcaata gcacatatta caaaactggc
      841 aattagggct aatgtacttt gtttaatatt aaaatctaaa tcagtaaagc gaagagataa
      901 tactgccaac agaccgatta ttcctactga actattttca tttccaaaaa gtgttgtaaa
      961 aaaagtgata aagataatac aaaaggctaa gcagaaaata tttcttagga tcattgctgc
     1021 aatataacga tgtttatctt tttttgtagg tgtatcacta attgcagctt ttaagttttt
     1081 aacatttaat tgcattgctt gataaatagt cattattgtc ctccttttaa ggttatatta
     1141 gattttttta ttaagttgat taattgtgta aaatcatcag atggtaattt cttttttaat
     1201 tgatagatgg gttctaaata ataagtatat atatcatcaa gatgttttct tccttgtgca
     1261 gttattttta aacaataact acgtttatca aatgggtgtg gacttttaag taacagttct
     1321 tttttattta attgctcaat aagacggctt actaatggtt ttgatacacc cattcgtttg
     1381 ctgataataa taggggtaat tatttcatca tcgataatta gtattgataa taaatctagn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacctcgg cgatttaata aatgcgaaaa
     1501 caaacgaatt tcttgtgagt aatttatcat ttttatccat tctggttctt tcataattcc
     1561 acctcttagt taactttatt aactatatat tattcataaa aaaagaattg tcaataacgt
     1621 caatttaaaa aataacaatt tttttatttt aggtatatac aacattcttg aggtgcgatt
     1681 gcatggtagg gatggaaaat atttggatag gaactggaag tttggaaatc gctgaataaa
     1741 gcgctttgag taactattag aataatagtt ctgattaatt tgcaactttt agcaagatga
     1801 tattgaatcg aatagctgat aggatggaag taacagagat ttttgataaa aatcttggtg
     1861 taagtaattt aggtaaaatc gttatattga atttaatcat cgtggtgttg ataaagtgaa
     1921 agaaatgaag ttgaaaatag tgtagcaagg attaaagtgg aatatgatta tataactaaa
     1981 gcatctaata aggaaggcta ttgcaaaagt aattgataaa tttgttttat cacgactgtt
     2041 tgaaaaataa tacttgtttt ctatttagaa ataagtaaaa tataatagac tcgtagaaaa
     2101 cgattgctac aaaagtagag gaaagtccag gcttccacaa gctgcgatgc ttgtagtgtt
     2161 tgtatgtgac gaatcaataa gtcacagtag cgtgagctaa cgacgggcct aagacctaag
     2221 cgtaagtatg gttgataagc cgtaaagtgc cacagagact attctaatcg aaagattagc
     2281 tgaaaagagg taaactctgc aaggaagaaa cccaaatttg gtaggggaat cgtctaaacg
     2341 gaaatgaacg cgaaggcgga tgcatgtgca tagataaata atcgtttgaa aaacagaact
     2401 tggcttacaa aacgagtcgt tatttagctg aatattttca gcttttttat ttgtctattt
     2461 ttttatcctc gtttataatt attaaagagg tatgaatatg aataaacatg ataaaacaag
     2521 atatgtattt gctcaagcaa ttaaaggttt aattaaggtt catcccttgg ataaaattgc
     2581 agtaactgat attgttacac gcagcgggat gacgcgtcag acattttatc gctactttaa
     2641 agataaatat g
//
LOCUS       DAOMTA010000403         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1715_length_2651_cov_2.067411, whole genome shotgun sequence.
ACCESSION   DAOMTA010000403 DAOMTA010000000
VERSION     DAOMTA010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_2651_cov_2.067411"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..761)
                     /locus_tag="PLK68_10500"
     CDS             complement(129..761)
                     /locus_tag="PLK68_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent methionine synthase"
                     /protein_id="HRM90873.1"
                     /translation="MIKENALKYLGYLDNQVDSNTEILLNECLKELEQVTPKFMYQIY
                     TLTHHPLTIKELNLTINYPDLIDLFDSCDRIVIIACTLGLQLDQQLRYYSKINLTKMT
                     VMDALASSYIEIKCDEYEAKQNFGKRTFRFCPGYGNVPLELNKNLANALNCSKHIGLT
                     VQESNLLLPQKSMIGLIGLGDEKLTKHCFSCVNKENCMYRKRGQRCYKKD"
     gene            complement(754..1644)
                     /gene="metF"
                     /locus_tag="PLK68_10505"
     CDS             complement(754..1644)
                     /gene="metF"
                     /locus_tag="PLK68_10505"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRM90874.1"
                     /translation="MKIIDLLHRRPTLSFEIFPPKNHDGDISSIYQTIDELAKLKPDF
                     ISVTYGAGGSTTENTVEIASKIKNEYSIEAVAHLSCIDATPEQLIRVLDSLKANNIEN
                     VLALRGDYPRGYDPMKAPHYYKYASELNDFISKNYPDTFCLSGACYPEVHQEAASLEE
                     DLIALKKKVDAGAEYLITQIFFDNNYYYRLVREARIRGINVPIIAGIMPATNSKSLLN
                     IAKLSGCNIPYNLSASIERFKSNPQAMKEVGMNYATNQIIDLITNGVDGIHLYTMNKP
                     ETVHEILKRTSNILAEFKND"
     gene            complement(1717..2265)
                     /locus_tag="PLK68_10510"
     CDS             complement(1717..2265)
                     /locus_tag="PLK68_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HRM90875.1"
                     /translation="MNQLELSEIIDKVVNKSDLTTKDIPSLDLYMDQIMTLFDDHLQD
                     NKRFVDDKLLTKTMINNYSKAGVIKPVKGKKYTKEQIIGMLLVYNLKNTITIQEIKQV
                     LAPVYANDESLENIYDQFIEIKKFQSDQLKPLVLKTVENFNLDIDNDNQRLISIMALS
                     SLSNQLTNIVQGIIDNYYIESE"
     gene            2391..>2651
                     /locus_tag="PLK68_10515"
     CDS             2391..>2651
                     /locus_tag="PLK68_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRM90876.1"
                     /translation="MRFFKKAMDPVSSETHFIGAVLSLATLVMMMIIAIIEGSDHLTI
                     IGIIVFGLSSIALYSASCLYHYYNANEDNKIKLILRKLDHSMI"
BASE COUNT          853 a          420 c          382 g          996 t
ORIGIN      
        1 atttaagtga attgtttgaa ttagtttggg gtcagtaata tttaaacatt ctggaatatc
       61 accagcttta agtccagcat cttgaagttg cgtccccatc gcaccatcaa aaaccaaaat
      121 atcatttttt aatctttctt gtaacatctt tgtcctcttt tccgatacat acaattttct
      181 ttattaacac atgaaaagca atgtttagtc aatttttcat ctccaagacc aattagtccg
      241 atcattgatt tttgcggtag cagcagatta ctttcttgaa cagtcaaacc aatatgtttg
      301 ctgcaattta atgcgttagc aagatttttg tttaattcga gtggaacatt accatatccc
      361 ggacaaaaac ggaaggtccg tttaccaaaa ttttgctttg cttcatattc atcacattta
      421 atttcaatat aactactagc taaggcatcc atcacagtca ttttcgttaa atttattttt
      481 gaataataac gtaactgttg atctagctgt aaccccaaag tacaggcaat tataacgatt
      541 ctatcgcaac tgtcaaataa atcaattaaa tcgggatagt taatcgttaa gtttaattct
      601 tttatagtta agggatgatg agttaaggtg tatatttgat acatgaattt gggtgttact
      661 tgttctaatt ctttaagaca ttcatttaaa agaatttcag tattagaatc tacttgatta
      721 tctaaatagc ccaaatattt taaagcattt tctttaatca tttttaaact cagctaaaat
      781 attactagtt cgttttaaaa tttcatgtac agtctccggt ttattcattg tataaagatg
      841 aattccatct actccattag tgatcaaatc aataatttga tttgtcgcat agttcatccc
      901 aacttctttc attgcttgag gattgctttt gaaacgctca attgaggctg ataaattata
      961 aggaatatta caaccactta atttagcaat atttaataac gactttgaat ttgttgcagg
     1021 cattattcct gcaataatcg gaacattgat tcctctaatt cgagcttctc taaccaaacg
     1081 ataataatag ttgttatcaa agaaaatttg tgtaattaaa tattctgctc cagcatcaac
     1141 ctttttcttt aaagcgatta aatcttcttc caatgacgct gcttcttggt gtacctcggg
     1201 ataacatgca ccacttaaac aaaaagtatc aggataattt ttactaataa aatcatttaa
     1261 ttcactagca tatttataat agtgcggagc cttcatcgga tcgtatcctc ttggataatc
     1321 tccacgtaaa gctaagacat tttcaatatt gttagctttt aatgaatcta gaacgcgaat
     1381 taactgttct ggtgtcgcat caatacaact taaatgtgca actgcttcaa tactatattc
     1441 gtttttgatt ttagatgcaa tttctaccgt gttttcagtt gttgaccccc cagccccata
     1501 tgtaactgaa ataaagtcag gtttaagttt tgctaattca tcaattgttt gatagattga
     1561 gcttatatcc ccatcgtgat ttttaggggg aaagatttca aacgacaaag ttggtcgccg
     1621 gtgtaataaa tcaataattt tcatgtgtag tctctcctta aataaattac gttaattata
     1681 agaaaaacaa gaggtctagt caactaaaac cccttgttac tcactttcaa tataatagtt
     1741 atcaatgatt ccttgaacga tatttgttaa ttgattagac aatgatgata aagccatgat
     1801 gctaatcaac ctttggttat cattatcaat atctaaatta aaattttcaa ctgttttcaa
     1861 tactaatggt tttaattgat cgctttgaaa cttttttatt tcaataaatt gatcataaat
     1921 attttctaac gattcatcat tagcataaac aggagccaaa acttgtttta tctcttgaat
     1981 agtaatcgtg tttttcaagt tataaacaag aagcattcca atgatttgct cttttgtata
     2041 tttctttcct tttactggtt taattactcc ggctttagaa taattgttga tcattgtttt
     2101 ggtaagtaat ttatcatcaa caaagcgttt attgtcttga agatggtcat caaacaaagt
     2161 cataatctga tccatatata gatctaatga tggaatatct tttgtggtta aatcgctttt
     2221 atttacaact ttatcaatta tttctgataa ttctaattga ttcataaaat cacctcatgg
     2281 atattttata atatttgagt taatgtttca agatgtggtt gacatatctt ataataatga
     2341 tatcataaat acataatata gttattaaaa ctacttaagg aggttgagat atgagatttt
     2401 ttaaaaaagc gatggatcca gttagtagtg aaacgcattt tattggtgct gttttatcat
     2461 tggcaacact agttatgatg atgattattg ctattattga agggtcagat cacttaacta
     2521 taattggaat tattgttttt ggactatcgt caattgcctt gtatagtgcc agttgtcttt
     2581 atcactatta taacgcgaat gaagataata aaattaagct gattttacga aaactagatc
     2641 attcgatgat t
//
LOCUS       DAOMTA010000404         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1719_length_2649_cov_1.956438, whole genome shotgun sequence.
ACCESSION   DAOMTA010000404 DAOMTA010000000
VERSION     DAOMTA010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1719_length_2649_cov_1.956438"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    207..259
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(260..1668)
                     /gene="rny"
                     /locus_tag="PLK68_10520"
                     /pseudo
     CDS             complement(260..1668)
                     /gene="rny"
                     /locus_tag="PLK68_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534398.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
     gene            1968..>2649
                     /locus_tag="PLK68_10525"
     CDS             1968..>2649
                     /locus_tag="PLK68_10525"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRM90877.1"
                     /translation="MSKTLVLAEKPSVGRDIARVLQCNKNVNGGLEGNKYIVTWALGH
                     LVTLADPEKYDKHYQKWDLNDLPIMPEKMQLVVIGKTAKQYNAVKNLMNRNDVKDIII
                     ATDAGREGELVARWIINKAHIKKPMQRLWISSVTDKAIKEGFKKLKNAREYEALYHSA
                     YSRSIADWIVGINATRALTCKYNAQLACGRVQTPTLAMIAAREEEIKDFVPRNYYGIE
                     IISSQINWT"
BASE COUNT          822 a          480 c          383 g          911 t
ORIGIN      
        1 aatacgatgc aaataatctt caatcatttc acgtccaatt gaaccaaata catcaccaat
       61 aaataatatt ttcattttat ttccctttca aataaaagga taggctatgc ctatccctat
      121 ttagcaactt cacttgctct tacttcacga attactgtaa ctttgatatg tcctggatat
      181 gtaagctctt cttcaatctt cgttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnna ttcgaacttc acgtccagct tgaattgcaa aaacccgatc
      301 aactccatca aagctttttg ccatttcttc aagtttttct agtcgttgaa tatagttttc
      361 aattgtctca ctacgactac ctgggcgtgc tgcactcaaa gtatcagcag cagcaaccaa
      421 aattgatatt acacttgttg caggtacatc accatgatgt gattcaattg catttacaac
      481 tgtagcatgt tcaccatgtt ttttagcaaa ttttgctcct aattccacat ggctcccttc
      541 catttcgtgg tcaactgcct taccaagatc atgtaataaa ccagcccgtc gagctagttg
      601 ttgatttaaa cctaattcag cagccatgat tcctgctaaa tgagcaactt ccaatgaatg
      661 ttgtaaggcg ttttgaccat aactagtacg atacttaagt tttcctaaca tcatgataat
      721 atctttatca atttttgaaa ttcctaattc aaaaacggca tcttcacctg ttttgcgaat
      781 cacttcatct aattcatttt tagttttttg aactacttct tcgattctac ctggttgaat
      841 tcggccatca cgaatcaaag tctccaatga taatctagca atttctcttc taacaggatc
      901 gaagcatgaa attgtaatag cttcaggagt atcatcaatt attaaatcta ccccagtaca
      961 ttgttcgatt gcttttatat tgcgaccttc acgtccaata attcttccct tcatttcttc
     1021 acttggaaga gcgacaacag aaactgttct ttcaattgtt tcttcttgag cataacgatt
     1081 aatcgcatta gcgattatat cacgagaaag cagacttgct tttgatctag cttcctcctc
     1141 ttgatcttta atataagccg tcatctctgt agcagtttgt tgttcaacct gcttaaataa
     1201 ttcttgctta gcttcatttg ctgacattgc agcaattttt tctaattcac caattttagc
     1261 attgattttt tcttttaatt cagcttctag tttacctagt ccggcttctc tttcgtctaa
     1321 ttgtactact ttttgcgcta aaacatcttc tttcccttga acagcaatat cacgacgatc
     1381 aactgtttgt tcacgttgta aaagtttatt ttcaagttca ttaatttctt gtttttgttc
     1441 tttagcttct ttttcagctt gaagttttaa ctcatatgct tctgtcttag catctaaaat
     1501 tgcttcttta acaagattgt ctgctttggc agttgcatca tctataatct ttgcagcact
     1561 tacgttagct ttagagattt ttaatttatt aattaaataa ttaataaaaa aacctaaagc
     1621 cacagctaga acataggtta aaatagaaaa ggcaatattt cctggcatat ttgtcctcca
     1681 ttttcttcta tgtaaattat ttttatgaat ctataggaat tcattatgtg acaagcacaa
     1741 taatattgta taatattttg acatcattta caatgaaaag tgtttcgagg atattttttt
     1801 gttaaagtcc tataacaaca aattttaggt gattttaaga taataatttt taaaaagaga
     1861 aatatctatg attaaatcaa caaaaaaaga tagcggtatc attttataat ttagtttcat
     1921 atcagtagtt tattatgata gaatatttaa gaaatgaggt aaaaaaaatg tcaaaaacac
     1981 tagtattagc agaaaaacca tcagttggac gcgatattgc acgagttcta caatgcaata
     2041 aaaatgttaa tggtggtctt gaaggaaata aatatattgt tacatgggct ttaggtcact
     2101 tagtaacatt agctgaccct gaaaaatatg ataaacatta tcaaaagtgg gatttaaatg
     2161 atcttcccat catgcctgaa aaaatgcaat tagtagttat tggtaaaact gctaaacaat
     2221 ataatgccgt caaaaacctt atgaatcgta atgacgttaa agatatcatt atagcaaccg
     2281 acgccggtcg tgagggagaa ctagtagctc gatggatcat taataaagca catattaaaa
     2341 agccaatgca gcgattatgg atttcatcag taactgataa agcaatcaaa gaagggttta
     2401 aaaaattaaa aaatgctcgt gaatatgaag ctttatatca tagcgcatat agtcgaagta
     2461 ttgctgactg gatcgtcgga atcaatgcta ctcgagctct tacctgtaaa tataacgctc
     2521 aattagcttg tggtcgtgtt caaaccccta cactagcaat gattgcagct cgtgaagaag
     2581 aaattaaaga ttttgttcct cgcaattatt atggtataga aatcattagt agccaaatta
     2641 actggactt
//
LOCUS       DAOMTA010000405         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1721_length_2647_cov_2.530864, whole genome shotgun sequence.
ACCESSION   DAOMTA010000405 DAOMTA010000000
VERSION     DAOMTA010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_2647_cov_2.530864"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLK68_10530"
     CDS             complement(<1..861)
                     /locus_tag="PLK68_10530"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRM90878.1"
                     /translation="MMKEKVILAYSGGLDTTAIIPWLKENYDYEVICCCIDVGQAEEL
                     NGLEERAKACGATKLYIKNVVDEYVKDYIMPTVQADAVFEYKYLLGTATARPLIAKIL
                     VDVARQEGATAICHGATGKGNDQIRFELGIKALAPDLKIIAAWRDPKWNMDSRESEIE
                     YCQAHGINLPFSADSSYSRDRNIWHISHEGLELENPENAPNYDHLLVLSTSPEKAPEE
                     PEHVVIEWEKGFPVKLNGKAKSLTAILEELNEIGGKHGIGIIDIVENRVVGMKSRGVY
                     ETPGGTILMEA"
     gene            1018..2154
                     /locus_tag="PLK68_10535"
     CDS             1018..2154
                     /locus_tag="PLK68_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM90879.1"
                     /translation="MDGVKIDYKKILEIVLIGIGCYWALNNFQIILDIFNSILAVIMP
                     FLLGIMIAFILNVLMIRIEKILSRFILDKKYTSFKRVISIIVSLLIVIGVIGIIITLI
                     IPELTNAIKVIVKSFPETFEQLQVWINQNGNSFPQLETWINSVDLNSIASELSGLFKI
                     GLTGMLGSTVDVISMFFTSILNLVVGIVFALYILMSKETLKRQSHKLIDAYIPKRISV
                     KLLEVGTLARTTFSNFVIGQTVEAFILGTLCAVGMAVLNLPYAPMVGSLVGITAFVPI
                     VGAFIGGGIGAFMILTVDPMQALIFIIFLVVLQQLEGDLIYPRVVGSSVGLPSIWVLF
                     AVTVGGGLWGITGMLFSVPVLSVVYALIKGHVNKSGTKPKITNN"
     gene            2374..>2647
                     /locus_tag="PLK68_10540"
     CDS             2374..>2647
                     /locus_tag="PLK68_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90880.1"
                     /translation="MLKIENLTKTYGQKKAVDNLSLEIENGHIYGFIGHNGAGKTTTL
                     KSIAGIMEFDQGNIYIDNKSIKEEPLACKKVMAYIPDNPDLYEYLTG"
BASE COUNT          871 a          376 c          448 g          952 t
ORIGIN      
        1 agcttccatt aaaatagttc ctcctggtgt ttcataaact ccacgagatt tcattccaac
       61 aacacggttt tctacaatat caatgattcc aattccgtgt tttccgccaa tttcattcaa
      121 ttcttctaag attgcagtca atgattttgc tttaccattt aatttaacag ggaatccttt
      181 ttcccattcg ataactacat gctctggttc ttcaggtgcc ttttcaggcg atgtagataa
      241 cactaataag tggtcataat ttggtgcatt ttcaggattt tccagttcta atccttcgtg
      301 agaaatatgc caaatattac gatcacgtga ataacttgaa tcagcagaga atggtagatt
      361 gataccatga gcttgacagt attcaatttc agattcacga gagtccatat tccattttgg
      421 atcacgccat gcagcaatga tctttaaatc aggtgcaaga gctttgatac cgagttcaaa
      481 acgaatttga tcgttacctt taccggtagc accgtgacaa atagctgttg ctccttcttg
      541 acgtgcaaca tcaactaata ttttagcaat taatggtcta gctgttgctg ttcctaataa
      601 atacttatat tcaaatacag catcagcttg aactgttggc atgatataat ctttgacata
      661 ttcatcaact acatttttaa tatataattt agttgcccca catgctttag ctcgttcttc
      721 taatccgttt aattcttcag cttgaccaac atcaatacaa caacagatta cttcataatc
      781 ataattttct tttaaccaag gaataatagc agtagtgtct agtcccccag agtaagcaag
      841 aataactttt tctttcatca tttaattccc ccttaaaata actttaaatt atcattataa
      901 taaactatag aattcataat tgcaatattt tctcgtaatt atgcaatatt ttgttgattg
      961 ttcatattta agcaatagtg gtaaaataag attaactaaa gtaatcggga ggataaaatg
     1021 gacggagtga aaatcgacta taaaaaaata ttagaaatag tattgattgg tatcggatgt
     1081 tattgggctt taaataattt tcaaataata ctagatattt ttaatagtat tcttgcagta
     1141 ataatgcctt ttttattagg gattatgatt gcttttatct taaatgtatt aatgattaga
     1201 atcgaaaaga ttttatctag atttatttta gataaaaagt atactagttt taaacgtgtg
     1261 atcagtatta ttgtttcatt gttgattgtt attggggtaa ttggaataat tattacttta
     1321 attattccag aattaacaaa tgcgataaaa gtaatcgtta aatcttttcc agaaacattt
     1381 gaacagttac aagtgtggat caatcaaaat ggcaatagtt ttccgcagtt agaaacatgg
     1441 atcaatagtg ttgatttaaa cagtattgca agtgaattaa gtggtttatt taaaattgga
     1501 cttactggaa tgctcggttc aactgttgat gtcatttcaa tgttctttac aagtattctt
     1561 aatttagttg tgggaattgt atttgccctc tacatattaa tgagtaaaga aactttaaaa
     1621 cgccaaagtc ataaattaat cgatgcctat attccaaaac gtatttcagt aaaattgtta
     1681 gaagtgggga cactcgctcg aacaactttt tcaaattttg tgattggtca aacagttgaa
     1741 gctttcattt tgggaacttt gtgtgccgtt ggaatggctg tgttaaatct gccttatgct
     1801 ccaatggtag gctctttagt agggatcacg gcttttgttc caattgtagg ggcatttatc
     1861 ggtggtggca taggggcatt tatgattttg acagttgatc caatgcaagc attgattttt
     1921 atcattttct tagttgtgtt acaacaactg gaaggagatt taatttatcc acgagtagta
     1981 ggctcttcag taggtttacc gtcgatctgg gtactttttg cagtaacggt tggtggagga
     2041 ttatggggaa ttacaggaat gttatttagt gttccggttc tttcagtagt ctatgctctt
     2101 attaaggggc atgttaataa aagcggtact aaacctaaaa taactaataa ttaaatctaa
     2161 gtaattttta ttaatgaaat gaatatcctg ttaaagcagg atattttttt aataactatt
     2221 tagaaaatat tctaaatagt ttaatattat gttacaatct atttagataa tattctaaat
     2281 agttacaata tcacttgcaa ttaggatata agtaggatat tatgataata taaataataa
     2341 aaataatttt atttatgaaa tgaggagatt aaaatgttaa aaattgaaaa tttgacaaaa
     2401 acttatggtc aaaaaaaagc cgtcgataat ttatcattag agattgaaaa tggtcatatt
     2461 tatggtttta ttggacataa tggagctggt aaaacgacta cattaaagtc gattgctgga
     2521 attatggaat ttgatcaagg gaatatttat attgataata aatcaattaa agaggagccg
     2581 ttagcatgta agaaggtaat ggcctatatt cctgataatc ctgatctata tgaatattta
     2641 acaggaa
//
LOCUS       DAOMTA010000406         2643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1726_length_2643_cov_1.901082, whole genome shotgun sequence.
ACCESSION   DAOMTA010000406 DAOMTA010000000
VERSION     DAOMTA010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_2643_cov_1.901082"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..295)
                     /locus_tag="PLK68_10545"
     CDS             complement(173..295)
                     /locus_tag="PLK68_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90881.1"
                     /translation="MAINNLAARQFYQWCQSDNWETLVNQDTEALLRRIDLGLP"
     gene            complement(838..>1178)
                     /locus_tag="PLK68_10550"
     CDS             complement(838..>1178)
                     /locus_tag="PLK68_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM90882.1"
                     /translation="SGIKIVLEETRLEEYIKDTDIVITGEGRLDFQTAMGKAPIGVAK
                     LAKKYHKPVIAFAGSVTADANECNHQGIDAYFPIVRGVTTLEEAMESSNAKNNLIATV
                     EQVFRLWKIK"
     assembly_gap    1179..1188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1189..>1787)
                     /locus_tag="PLK68_10555"
     CDS             complement(<1189..>1787)
                     /locus_tag="PLK68_10555"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM90883.1"
                     /translation="VTGPLGTRVVCQYGIITETKTAVIEMASAAGITLVAPDQRNPLL
                     TTTYGVGEIIADAITMGCRHFIIGIGGSATNDGGIGMLQALGYRFLDKYGNQVTYGAQ
                     GLEQLVEIDDSGVVSSLNECTFKIACDVNNPLCGKLGSSAVYGPQKGATAEMIKKMDK
                     GLNDYAKLAKLKYPNADPELPGTGAAGGLGFAFLTFLNA"
     assembly_gap    1788..1797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1798..1957)
                     /locus_tag="PLK68_10560"
     CDS             complement(<1798..1957)
                     /locus_tag="PLK68_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM90884.1"
                     /translation="MKIVIAIDSLKGSLSSIEAGEAIKAGIEHVYDDAKVIVSPLADG
                     GEGTVEALT"
     gene            2085..>2643
                     /locus_tag="PLK68_10565"
     CDS             2085..>2643
                     /locus_tag="PLK68_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HRM90885.1"
                     /translation="MYINNQLATQIVETVKDICDHDINFIDCQGIIYASTDIKRIGSF
                     HEAGLLAIKEKRIVEVYDDTSFNGSLQGVNLPVYYHDQIIAVIGISGKPDKVKKYAYL
                     AQKITNLLIREKELNEFNRSQAEKMHYLLQSLLDQENLDSQYFSDMLKYFQLDLKNNY
                     RLLIILAKRLVNEQSIIQELHSLAIP"
BASE COUNT          835 a          555 c          351 g          882 t
ORIGIN      
        1 acggcatttg ataataacga aggatgctta cccattggaa agataatcat tttaacaggt
       61 ggatgctcgt atataatttt ttcatattct tctatgatat cactgtgtaa catcgcatcc
      121 ttcccactag taattaacaa caatagtacc tttaaagtat ttaaaggtac ttctacggta
      181 aacctaaatc aattcgtctt aacagtgcct cagtatcttg attcactaaa gtctcccaat
      241 tatcactttg acaccattga taaaattgtc ttgctgcaag attgtttatt gccatgactc
      301 actcctcgat taatgctttg gcaaatcccc gacgaagagt tcgcccatca aaactatctg
      361 ccactacaca agtgattaaa tctgagcgtc ttaatgcagc attaactgca ccccaaacac
      421 ctccacttgt ttcgattaaa gtaacatctt taatatttaa ggattctaat aaagcgatta
      481 cctgatcagc ttcttgtcac cataagtcag taggaaacct cgctattcac tgtaatttac
      541 catgtcctaa aaaatcaatt aaaataagtt tatactcatc ttgatatagt gataaaattt
      601 attcaaacat tccagctgat gctgtatttc catgcaaaaa caatagcggt tactcttccc
      661 ctaacatctg acaataaaca tttttttctt gtatttaaaa tatatcatat tctgcctcct
      721 gaatttttta tcgagcaagg ctataaacag taaagccctg catgctactg tttaatccag
      781 attcccattc ccatcgctcc tttttgaaca aattttatat acatttatca tatcatctta
      841 ttttatcttc cacaaacgaa acacctgttc aacagtagct attaaattat ttttagcatt
      901 actgctctcc attgcttcct ctaaagttgt aacaccacga acaatcggaa aataagcatc
      961 gataccttgg tgattacatt cattagcatc cgctgtcaca cttccagcaa aagcaattac
     1021 tggcttatga tacttcttcg ctaatttagc aacccctatt ggagctttac ccatagctgt
     1081 ttgaaaatca agacgccctt caccagtaat aacaatatca gtatccttta tatattcttc
     1141 taatcgtgtt tcttctaaaa caattttaat acctgattnn nnnnnnnngt gcatttaaaa
     1201 atgtcaaaaa tgcaaagcct agaccaccgg cagctccagt accgggtagt tccggatcag
     1261 cgttaggata ttttaatttc gctaatttag cataatcatt aagcccctta tccatttttt
     1321 ttatcatttc agcagtcgcc cctttttgcg gtccataaac agcactactc cctagtttac
     1381 cacatagcgg attattaaca tcacaagcaa ttttaaaagt acactcgttc aacgaagaaa
     1441 caaccccact gtcatcgatt tcaactaatt gttccagtcc ttgtgctcca tatgttactt
     1501 gatttccata tttatctaaa aaacggtaac ctagtgcctg taacattcct attcctccat
     1561 catttgtggc actaccgcca atccctataa taaaatgccg acatcccatt gtaattgcat
     1621 cagcaataat ttcaccaaca ccgtaagttg ttgtcagtaa gggatttctc tgatctggag
     1681 ctaccaaagt aattcctgca gcactcgcca tttcaataac tgccgtcttc gtttctgtaa
     1741 taattccata ttgacaaact actctagtgc ctaatggccc cgtaactnnn nnnnnnncgg
     1801 ttaatgcctc aaccgttcct tctccaccat cagctagtgg gcttactatt acttttgcat
     1861 catcatatac atgctctatt cctgctttga ttgcttctcc agcttcaatt gaagataaac
     1921 ttccttttaa cgaatcgatc gcaataacta ttttcattat tttatccccc ttgtttttct
     1981 tagttataat ataacaaatt atttttataa ataatatgtt ataattaaca tgttatttaa
     2041 ataaaatgca tatttattta gtacttatac caaaggagga ttatatgtat attaataacc
     2101 aattagctac tcaaatcgtt gaaacagtta aagatatttg tgaccacgat attaatttta
     2161 ttgattgtca aggaattatc tatgccagta cagatataaa aagaattggt tccttccatg
     2221 aagctggtct tttagccatc aaggagaaaa gaattgtcga agtgtatgat gatactagtt
     2281 ttaatggtag tcttcaggga gttaatttac cagtctatta ccacgatcaa attattgctg
     2341 ttattggaat cagtggtaaa cctgataaag taaaaaaata tgcctattta gcacaaaaaa
     2401 tcacaaatct attaattcga gaaaaagagc tgaatgaatt taatcgtagc caggctgaaa
     2461 aaatgcatta tctgttacaa tcattactag accaagaaaa ccttgactcc caatattttt
     2521 cagatatgtt aaaatatttt cagttagatt taaaaaacaa ttatcgtctt ttgattattt
     2581 tagcaaaacg gctcgtaaat gagcagtcta ttatccaaga acttcatagc ttagccattc
     2641 cgg
//
LOCUS       DAOMTA010000407         2643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1728_length_2643_cov_1.741886, whole genome shotgun sequence.
ACCESSION   DAOMTA010000407 DAOMTA010000000
VERSION     DAOMTA010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_2643_cov_1.741886"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLK68_10570"
     CDS             <1..307
                     /locus_tag="PLK68_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM90886.1"
                     /translation="ITKNRGCIPNLDSEMMVEVACRVGANGVEPLAMDPVDTFYKGLL
                     ENQYAYEKLTVDGFLECNKLKLLQALVLNRTVVDTDLAKVILDDLIEANKAFWPEFK"
     gene            439..1230
                     /locus_tag="PLK68_10575"
     CDS             439..1230
                     /locus_tag="PLK68_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM90887.1"
                     /translation="MQLNELVNLHFDDLNESDLYIWNYINNHREVCTKITIEDLGKKC
                     NVSRTTILRFSKKIGLEGFAELKYYLRNEQTLADVGLGKTVNIYALFENHAKMIRELQ
                     NRDYNTCCRMIDHAKRIFVVSSGTIQRSVAKELSRQFLKLGIIMTLVNGDSEINMLAR
                     AITADDLLIIISLTGESESVIRIAKVAKTVGSKTISITRINANSLSLLADEPIYIFFG
                     DFPIVFETAYTSPTMFFALTELLFANYHNYQQKKLFQQGNQNYAE"
     gene            complement(1333..2349)
                     /locus_tag="PLK68_10580"
     CDS             complement(1333..2349)
                     /locus_tag="PLK68_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM90888.1"
                     /translation="MDNHKKKLSIKDIAAISGVSIATVSRVLNNKGGYSKETEEKINA
                     LAKSYGYVSNMNAKSLRESKSQTIGLIVPDISNDFFATLALHIENYSAKHNYSVFICN
                     SANNVQKEKDYFKSLASKCVDGIICISGLNKLTEDIIYNDIPIVCIDRYPENNKTIPR
                     ISSDDIHAAFLATEHLIKKGCRDIVFISSFTADYEKKERYLGYKKALDTYGIPLDKNY
                     ILQTQGKEPSQIEAEILITDFLKTQRRIDGIFASSDLAALGALYALKRANLKVPEQVK
                     LIGFDNTLYSRLPTPSISTIERNPKMLAEKGCEVLLNMIQGKDIGSIDTIVPVVLVER
                     ESSK"
BASE COUNT          898 a          364 c          446 g          935 t
ORIGIN      
        1 tattactaaa aatagaggat gtattccaaa tttagatagt gagatgatgg tagaagtagc
       61 ttgtcgggtt ggggcaaatg gtgttgaacc actagctatg gatccagtag atactttcta
      121 taaaggatta ttagaaaatc aatatgcata tgaaaaatta acagttgatg gattcttaga
      181 atgtaataaa ttgaaattat tacaggcgtt agtattaaat cgtacagtag ttgatacgga
      241 tttagctaaa gtgattctag atgacctaat tgaagctaat aaagcatttt ggcctgaatt
      301 caaataataa cactccctta ctatatctct ttatttgaat tttgttgtag taatagcgaa
      361 gtttttaact tcgctatttt atcatttatt gcaaaaaatt tttttgttat acttaaataa
      421 taaaggacgg atgaaaagat gcaattaaac gaattagtaa atcttcattt tgatgatttg
      481 aatgaaagtg atttatatat atggaattat attaataatc atcgtgaagt atgcacaaaa
      541 atcacaattg aagatttagg aaaaaaatgt aatgtttcaa ggacaacgat attacgtttt
      601 tctaaaaaaa ttgggttaga gggatttgca gagctaaaat attacttgcg caatgaacag
      661 acccttgccg atgtaggatt aggcaagaca gtaaatattt atgcattatt tgaaaatcac
      721 gctaaaatga ttagagaatt acaaaatcgt gattacaata cctgttgtcg tatgatcgat
      781 catgctaaac gtatttttgt agtttctagt gggacgattc aaagatcagt ggcaaaagaa
      841 ttgtcgcgcc aatttttgaa attaggaatt attatgacat tagttaatgg tgatagtgaa
      901 attaatatgt tagcacgagc aatcactgcg gatgatttat tgattattat ttctttgacg
      961 ggagaaagtg aatcggtaat tagaattgct aaggtggcca agacagtagg aagcaaaaca
     1021 atctcgatta ctaggataaa tgctaacagt ttatctttgc tggctgatga accaatttat
     1081 atcttttttg gtgattttcc tattgttttc gaaacagcat atacttcacc aacaatgttt
     1141 tttgcattaa cggaattatt gtttgcaaat taccataact atcaacaaaa aaagttattt
     1201 caacaaggaa atcaaaatta tgctgaataa ctaaaaaacc tgcgcttagt ggtacaatct
     1261 tgtatttaga gctgatgaaa cttaattatt tcattgctcc tttgaaaaga ttgatcattg
     1321 atgcggaggt ttttatttac tcgactctcg ttctactaag acaacaggaa caattgtatc
     1381 gatcgaacca atgtcttttc cttgaatcat attcaataga acttcacagc ccttttcagc
     1441 aagcatcttt ggattccttt caattgttga aattgagggg gtaggtaacc gtgaatataa
     1501 agtattatca aatccgatta atttgacttg ttcgggaact ttaagatttg ctcgttttag
     1561 tgcatacaag gctcctaagg cagctaaatc actagaagca aaaataccat caatcctgcg
     1621 ctgtgttttt aaaaagtctg ttattaatat ttcagcttca atttggcttg gttcttttcc
     1681 ttgagtttgt aagatataat ttttatcaag tggaataccg taagtatcta aagctttttt
     1741 atatcctaaa taacgctctt ttttctcata atcagcggtg aaactagaaa taaaaacaat
     1801 atctctacag ccttttttga tcaaatgttc agtggctaaa aaagctgcat gaatatcatc
     1861 actagaaatt cgaggaatag ttttattatt ttcaggatag cgatctatac atacaatggg
     1921 aatgtcatta taaatgatgt cttctgttaa tttatttaaa ccactgatac aaataatgcc
     1981 gtcgacacac ttgcttgcta aacttttaaa ataatctttt tctttttgaa cattatttgc
     2041 actattacaa ataaaaacag agtagttatg ttttgctgaa taattttcaa tatgtaatgc
     2101 tagagtcgca aaaaaatcat tgctgatgtc tggaacgatt aaaccaatcg tctgtgattt
     2161 cgattctcta agacttttgg cattcatatt agaaacatag ccatatgatt tagctaaagc
     2221 attaattttt tcttctgttt cttttgaata tccgccttta ttatttaata ctctggaaac
     2281 agttgcaatg ctaactccag aaattgcagc aatatcttta attgatagtt tttttttatg
     2341 attatccatg ttatctcctt gcttgttaag attatataaa ttatacatga ataggctatg
     2401 aatgtaaata ttagcaggtt ttattaattt ttacgtaaac gtttacgtaa aaatgttgac
     2461 atttacctat ttgtatggta tttttaatct acgtaaacgt ttacttaaag taatggcgcc
     2521 gttatttttt taccagttag gttaaacgtt tacgcaaata aatgcaagaa aattgcatct
     2581 aaactataag agaggaaaat agtagaatag agatcattct ggctaaggaa agggtttatg
     2641 gat
//
LOCUS       DAOMTA010000408         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1729_length_2642_cov_2.224198, whole genome shotgun sequence.
ACCESSION   DAOMTA010000408 DAOMTA010000000
VERSION     DAOMTA010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_2642_cov_2.224198"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..389)
                     /locus_tag="PLK68_10585"
     CDS             complement(153..389)
                     /locus_tag="PLK68_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90889.1"
                     /translation="MLFIILEILNNIIMAVVGYHVKLRDSKTMKGVSMLNASVYNIGR
                     FVLPFIQAIMPSLTVTYLSTFNIGAIFMVLVASM"
     assembly_gap    700..709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(996..>1781)
                     /gene="rlmD"
                     /locus_tag="PLK68_10590"
     CDS             complement(996..>1781)
                     /gene="rlmD"
                     /locus_tag="PLK68_10590"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM90890.1"
                     /translation="RGLLRHVLIRRALVTDQTMVVLVCNDNVFKGSKNFCNELIKNFP
                     SIKTVVLNVNKRKTSVVLGNEEKILYGKGFIVDELCGLKFKISPKSFYQINHQQCELL
                     YSKALDLLNLTGQERIIDAYCGIGTIGMIVANRTKEVTGVELNKDAVKDAINNAKMNK
                     IENIKFINDDASAFMIKLAKQKQKVDCVIMDPPRSGSTQEFMDAVKILNPKQVVYISC
                     DPSTQVRDIKYFAKIGYRGEVMYPVDMFPHTSHVETIVLLSKI"
     assembly_gap    1782..1819
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1820..2158)
                     /locus_tag="PLK68_10595"
     CDS             complement(<1820..2158)
                     /locus_tag="PLK68_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM90891.1"
                     /translation="MKCLIEKKCGSCKYINTDYQRQLKIKTDYCKKLLKDNKLDMYEV
                     KDTIGMGHPYEYRNKIIVAFNHRYEFGFYEEDSHKIIPYDRCLLHEELSDMIIKKIQS
                     LLKRYRVSIYD"
     gene            complement(2278..>2642)
                     /locus_tag="PLK68_10600"
     CDS             complement(2278..>2642)
                     /locus_tag="PLK68_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM90892.1"
                     /translation="RGKYSEVAEVDPNRIIIVYIEIAPGQFIELFPKDEGQAPHDEWN
                     QRLGYSHFALLVEDIEKTYRELVECGVAIDTPISKGPSHTYQMWVHDPDGNKFEIMQY
                     TDKSFQVVGHIEEEISED"
BASE COUNT          892 a          479 c          282 g          941 t
ORIGIN      
        1 agtaaatatg ccttttttga atttggaatt tctatcctca taatcattag aataaagtca
       61 atcaaataaa tatctaaagc tattgatgaa aacaatgttt taaaaagatt tgattctaaa
      121 gtgattttct ccatttacaa catagtcact tatcacatag atgctaccag caccataaag
      181 attgctccaa tattaaaagt acttaaataa gttactgtca aagatggcat tatcgcttga
      241 ataaacggca aaacaaacct tccaatgtta tatacagaag catttaacat agagacacct
      301 ttcattgttt tggaatctct taatttaaca tgatagccaa caaccgccat aattatatta
      361 tttaaaattt ctagaataat aaaaagcatg atattttatg tcaatcgaat ctcacggttc
      421 ccgcaagcaa tgtccatgga catgtaatgt ttgttataag taaagcaaga acatacgaat
      481 cactttcttt aatatatcct cttaatttta taaaacatgc taaaaagata atcaaaataa
      541 ataccattgc ttgttgtatt actgacgtca taaattttta tcttctattc ttttgtaatt
      601 agtatgctta tacttgatgt atcgatttta ataattcaat acccgttttt gttttaaata
      661 ttttttctat aaaatttctt atatcttcaa cggaataatn nnnnnnnnng ctgctgtttc
      721 aagagctttt aagtcttgct gattcagttt tactaattca ccaatgtttt agcataagca
      781 tacactttta agaaatgttt aatgttatag ttatttccat tagcatattt aatcatttta
      841 tttgcaatat tataaatcat cacaatcctt cttaatctaa taattaatcc taataaaaga
      901 ataatcttca tatctattaa aataaagatt ttttatattc tcacttattt tcattattta
      961 ataaaaaaag cgcaactaaa ttacgctcta aacttttata tttttgatag cagtacgatt
     1021 gtctcaacat ggctagtatg agggaacatg tcaaccggat acattacctc accacgatag
     1081 ccaatttttg caaaatactt gatgtccctt acctgagtac ttggatcaca agaaatataa
     1141 acaacttgtt taggattcaa gatttttaca gcatccataa attcctgcgt actgccactg
     1201 cgtgggggat ccataattac acaatcaact ttttgcttct gtttagctaa tttaatcata
     1261 aaagcagaag catcatcatt aataaattta atattttcaa tcttattcat tttagcatta
     1321 ttgatggcat ctttaactgc atctttattt aattccacac cagtaacttc cttagttcta
     1381 ttagctacaa tcattccaat tgtcccaatt ccacaataag catctataat tctttcttga
     1441 cctgttaaat tcaataaatc tagagcttta gaatacagta attcacactg ttgatgatta
     1501 atttgataaa atgattttgg cgaaatctta aacttcaaac cacacaattc atcaactata
     1561 aatcccttac cataaagaat cttttcttca ttacctaaaa ccacactcgt tttacgttta
     1621 tttacattta atacaaccgt cttaatactt ggaaaatttt taatcaactc attacaaaaa
     1681 tttttggatc ctttaaatac attatcattg caaactagta ctaccattgt ctgatcagta
     1741 acaagagcac gtcgaattaa tacatgacgc aataatccgc gnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnna tcataaatac taactcgata tcgttttaat aagctttgaa
     1861 tcttcttaat aatcatatca ctgagttctt catgaagaag acaacgatca taaggaataa
     1921 tcttatgact atcctcttca taaaagccaa attcatatct atgattaaaa gcaacaatta
     1981 ttttattacg gtattcataa ggatgcccca ttccaatagt atccttgact tcatacatgt
     2041 ctaatttatt atcttttaac agcttcttac aataatctgt tttaatcttt aattgtctct
     2101 ggtaatcagt attaatgtat ttacagctac cacatttctt ctctattaaa catttcattt
     2161 tatcacctag caacagtata acataactaa agcaaaatac taatcaattc cttctctcat
     2221 tattatttct tcataaataa aaacaaggta tagtttgtta ccaaactata cctactatta
     2281 atcttcagat atctcttctt caatgtgccc cactacttga aatgacttat cagtatactg
     2341 cataatttca aatttattac catcaggatc atgcacccac atttgatagg tatgtgatgg
     2401 acctttagaa ataggtgtat ctattgcaac tccacattca actagttctc gataagtttt
     2461 ttctatatcc tcaaccaata acgcaaagtg tgaatacccc agcctctggt tccactcatc
     2521 atgtggcgct tgtccttcat cctttggaaa tagttcaata aactgtccag gggcaatttc
     2581 aatataaaca atgataattc tgtttggatc aacttcagct acttcactat atttaccacg
     2641 at
//
LOCUS       DAOMTA010000409         2641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1730_length_2641_cov_2.163960, whole genome shotgun sequence.
ACCESSION   DAOMTA010000409 DAOMTA010000000
VERSION     DAOMTA010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1730_length_2641_cov_2.163960"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1644
                     /locus_tag="PLK68_10605"
     CDS             202..1644
                     /locus_tag="PLK68_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM90893.1"
                     /translation="MDRNIKTSFKNVVDEYIVNRKAMFIPIAAVASFMLVGYAAADKE
                     KPEIKSNQIDLAYGEKFDVDSIDITDNKDSRDLIEVSADTSSLNVTQLGSYKVSVTAT
                     DSGSNVATKTVQVNVVDNEGPKFELLGSNQGYTIDVPVKGSTDFASYVKANDNVDGDV
                     TPFIEANTPLNTEVKGQQDITLKATDSSGNETVKTFTFAVSDLEAPVINLTQGENVTV
                     DYGSGFDLNNFVNVTDNLDGTLVPTVEGSIDTSKIDETQTLKISATDSSGNVSEANLN
                     VVVKDLSAPVISLTKSSITVNAGENVDFNAYIASAIDNKDGDVKANVKVDAPSTAKAG
                     TKTATYTVTDAAGNTGTASLSVKVNAVYGGAASNNYGNSVLSAAYSRLGCPYVWGADG
                     PNSFDCSGFVKWCYSRVGISLPHSSSAQKNAGTQISISQAQPGDILWKSGHVGIYIGN
                     GQYIHAPRTGDVVKISSVSGSGFVCAVRVK"
     gene            2070..>2641
                     /locus_tag="PLK68_10610"
     CDS             2070..>2641
                     /locus_tag="PLK68_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM90894.1"
                     /translation="MANDKINVNMFGTFSLSRGNVTVDLTKLLGKQLINLFQLLLLQK
                     GVVISKNNIIDILYPDSENPNSVVKFSIFRLRKDLKASGIFNEDEEVILTVKGGYQIN
                     PKLEWVMDTEEFYYNWDKIKYIDDISDKDLAYAQKVNHLYKGKLYITTNHSIWIEQML
                     EFFRSAYVNCVIKICKYYMKRKQFEKMMSI"
BASE COUNT          953 a          325 c          495 g          868 t
ORIGIN      
        1 tatgtttatg gtaatttcta tttaggacta tgtaaaatct tataaatttc ttaagaaatc
       61 ttaaaaaaat tattctcttg aaagctaagc ggagcttgcg atacagccaa aggacaaaaa
      121 tgaaagagct ttcagggcta gatttttcta aaaaatagta atataataac aattggatat
      181 attattgaaa ggaggtaacg aatggataga aatattaaga caagctttaa aaatgtagtt
      241 gacgagtata tcgttaatcg taaagcaatg ttcattccga ttgcagctgt tgctagtttt
      301 atgctagttg gttatgcagc agcagacaaa gaaaaacctg agattaagtc taatcaaatc
      361 gatcttgcct atggtgagaa atttgatgta gatagtattg atattactga taataaggac
      421 agtcgtgatt taattgaagt atcagctgac acttcatcat taaatgtaac acaattaggt
      481 tcttataaag tatcagttac tgcaactgat agtggttcta atgttgctac aaaaacagtt
      541 caagttaatg tagtagataa tgagggacca aaatttgaat tattaggttc aaatcaagga
      601 tacacaattg atgttcctgt aaaaggaagt acagatttcg ctagctatgt taaggctaat
      661 gataatgttg atggtgatgt aactccattt attgaggcta ataccccatt aaatacagag
      721 gttaaaggtc aacaagatat tactttaaag gctactgata gttcaggtaa tgaaactgtt
      781 aaaactttta cattcgctgt tagcgattta gaagctccgg ttattaattt aacccaagga
      841 gaaaatgtta ctgtagatta cggaagtggt tttgatttaa ataattttgt aaatgttaca
      901 gataacttag atggtacttt agtaccaact gttgaaggat ctattgatac ttcaaaaatt
      961 gatgaaacgc aaacattgaa aatttctgca actgatagtt caggaaatgt tagtgaggct
     1021 aacttaaatg ttgttgttaa agatttatca gctcctgtaa ttagtttaac aaaatcttca
     1081 attacagtta atgctggtga aaatgttgat tttaatgcat atattgcttc agcaattgat
     1141 aacaaagatg gtgatgttaa agccaatgtc aaagttgatg ctcctagtac agcgaaagct
     1201 ggtactaaaa cagctacata tacagtaact gatgcagccg gtaacacagg taccgcgagc
     1261 ttatcagtaa aggtaaatgc agtttatggc ggggcagcaa gtaataacta tggtaatagt
     1321 gttttatcag ctgcttattc tagattaggg tgtccatatg tatggggagc agacggtcca
     1381 aattcatttg actgttcagg ttttgtcaaa tggtgttact cacgtgtagg aatttcactt
     1441 ccacactcat cttcagctca aaagaatgca ggtacacaaa ttagtatttc ccaagcacag
     1501 ccaggagata tcttatggaa atcaggacat gttggtattt atattggtaa tggtcaatat
     1561 attcatgccc caagaactgg tgatgtagtt aagatttcat ctgtgtctgg ttcaggattc
     1621 gtttgtgctg taagagtaaa ataaaaattg aatataacaa ctaattaaag attacgattt
     1681 taaattcgta atcttttttg attctatcaa tataattagt attaccgcac ttataaataa
     1741 agttgacgta attaaaaata cagtgctata atgaatatgt tatttttata aaaaaattag
     1801 aatcgaggat catacttttc ctgcacggga aagaatagat tttcgtgcac aaaaaaaaca
     1861 cataaatttg ggtctattta cgtgttttat agtaagatta tatgcgaacg aaaacgtata
     1921 gttaaaccaa gtggtaagaa tttatcacta ataaattact attaaataaa aataatgggc
     1981 taatactgta atggggtttt agaatatgac tattttggtg gataaatttg tgggcaagta
     2041 acatatcttg aaaagtgagg gaattagata tggcaaatga taagattaac gtaaatatgt
     2101 ttggaacgtt ttcactttca cgtggaaatg taacagttga tttaaccaaa ttattaggaa
     2161 aacagttaat taatttattc caattattgc tgcttcaaaa aggagttgta atatctaaga
     2221 ataatattat tgatatttta tatccggata gtgaaaatcc aaatagtgtg gttaagtttt
     2281 ctatttttag acttcgtaaa gatttaaaag catcaggtat ttttaatgag gatgaagaag
     2341 ttattttaac tgttaaaggt ggttatcaaa tcaatcccaa gttggaatgg gtgatggata
     2401 ctgaggagtt ttattataac tgggataaaa taaaatatat tgatgatata agtgataagg
     2461 acctagcata tgctcaaaaa gtaaatcatc tatataaagg taaattatat ataacgacta
     2521 atcattctat ttggattgaa cagatgctag aattttttcg ctcagcatat gttaactgtg
     2581 ttatcaaaat ttgcaaatac tacatgaaaa gaaaacagtt tgaaaagatg atgtcaataa
     2641 a
//
LOCUS       DAOMTA010000410         2632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1738_length_2632_cov_1.866511, whole genome shotgun sequence.
ACCESSION   DAOMTA010000410 DAOMTA010000000
VERSION     DAOMTA010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_2632_cov_1.866511"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLK68_10615"
     CDS             <1..253
                     /locus_tag="PLK68_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM90895.1"
                     /translation="LSGGEKVLLKMAILMQHKVNLLILDEPTNHIDIETREILEDALS
                     NYSGTLLFVSHDRYFIDKLAQRKLVIENNTINSVYCDYK"
     gene            complement(378..1178)
                     /locus_tag="PLK68_10620"
     CDS             complement(378..1178)
                     /locus_tag="PLK68_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM90896.1"
                     /translation="MEYTQLNNGIKMPSIGFGVYQIPLLMTKQCVLDALAIGYRHIDT
                     ARNYGNEREVGEALIDSGISRAEIFLTTKIYGADSYRQACQYIDEALNKLQTKYIDLM
                     LFHWPSGNITETYRAMEDYYKAGKLKAIGLSNFFIHDFNTVISSCTIIPMVNQVEAHI
                     FHQRKVFQKDMNQYGIKLEAWSPLACGKNNIFGNLILEKIAKSHRKSTAQVALRFLNQ
                     KGIAIIPKSIHKDRIKENWEIFDFELTNDEMKEIELLDCDCSLFSWYE"
     gene            complement(1283..>1718)
                     /locus_tag="PLK68_10625"
     CDS             complement(1283..>1718)
                     /locus_tag="PLK68_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRM90897.1"
                     /translation="IVGLVLNFTGIYDLLIASQFGEMITGTLSQATTPIVSMILFILG
                     YDLNVDKKTLVPILKLMGIKIVYYALVIAGFFILFPAQMADKTFMMAPIIYFMCPTGF
                     GLMPVIAPLYKDEDDASFTSAFVSIFMIITLIVYTLVVIFIA"
     assembly_gap    1719..1728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1729..2294)
                     /locus_tag="PLK68_10630"
     CDS             complement(<1729..2294)
                     /locus_tag="PLK68_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRM90898.1"
                     /translation="MTALTTLFPVFFMLALGFIARVKGWITLEQKNGANAIIFKILFP
                     ILVLNLMCTATIETDHIKIIGYVFGVYLLALVAGKLLAPLAGKKYAHFSPYLLTVVEG
                     GNVALPLYLSIVGKSSNTVIFDIAGTVIAFIVFPVLIAKEAATGSSMKEMIKSIFTNS
                     FVIAVMIGLIIISGTFLAVVYTAKKECET"
     gene            complement(2334..>2632)
                     /locus_tag="PLK68_10635"
     CDS             complement(2334..>2632)
                     /locus_tag="PLK68_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM90899.1"
                     /translation="AHDILVEAYSPVGHGEMLKNNEVKLIADKYGVTIPQLCIKYCLQ
                     LDTLPLPKTADPGHMQNNADLDFIISDEDMMLLKNINKIKNYGEFSIFPVYGKI"
BASE COUNT          930 a          466 c          328 g          898 t
ORIGIN      
        1 cttatctggt ggtgaaaaag tattattaaa aatggctatt ttaatgcaac ataaagtcaa
       61 cttattaatt ttagatgaac caactaatca tattgatatt gaaactcgtg aaattttaga
      121 agatgcactt tcaaattatt caggaacatt attatttgta tcacacgatc gttatttcat
      181 cgacaaacta gctcaacgta aattagttat cgaaaataat acaatcaaca gtgtttattg
      241 tgactacaaa taaactataa aaaaggacga ctaatgaagt gaatctccct tatttggaca
      301 tgatagggaa ggttcatttc ttaacatcct cctttttttg ttattcttct ctaactaccg
      361 ctaaataaat tatttactta ttcataccag ctaaataagc tacaatcaca atctaataat
      421 tcaatttctt tcatttcatc atttgttaat tcaaaatcaa aaatctccca attttccttt
      481 attctatctt tatggatcga tttaggtata atcgcaatac ctttttgatt taaaaatctt
      541 aaagcaacct gagctgttga tttacgatgt gatttagcta ttttttctaa tattaaattg
      601 ccaaagatat tattttttcc acatgccagt ggtgaccacg cttcaagctt gattccatat
      661 tggttcatat ctttttgaaa aacttttctt tgatgaaaaa tatgtgcttc aacttgattg
      721 accataggaa ttattgtaca tgaactaata acagtattaa aatcatgaat aaaaaaattt
      781 gataagccaa tcgcttttaa cttgccggcc ttataataat cttccatcgc tcgatatgtt
      841 tctgttatat ttccagatgg ccaatgaaac aacattaaat caatgtattt tgtctgtaat
      901 ttatttaacg cttcatcaat atactgacat gcttgacgat aactatcagc cccatatatc
      961 ttagttgtta gaaagatttc agctctcgaa atacctgaat ctataagcgc ctctccaact
     1021 tctctttcat taccatagtt ccttgctgta tcaatatgtc gatacccaat tgctaaagca
     1081 tctaagacac attgctttgt cattaataat ggaatttggt atacaccaaa gcctatactt
     1141 ggcattttaa taccattatt taattgtgta tattccatat tcaatctttc tcctaatttt
     1201 taatttatat tcttatcaaa aggtcaaatg gactattatt tatcttaaag ctcctctaaa
     1261 tcaataagaa agcacgctta ttttaagcaa taaaaataac aaccaaagta tagacgatca
     1321 gtgtaataat cataaagata gaaacaaagg cacttgtaaa tgaggcatca tcttcatctt
     1381 tatataatgg ggcaataaca ggcatcaatc caaatccagt gggacacata aaatagataa
     1441 ttggagccat cataaaagtc ttatcggcca tttgggcagg aaataaaata aagaatcccg
     1501 caataaccag cgcataataa acaattttaa ttcccattaa cttaagaatc ggtactaatg
     1561 tttttttatc aacatttaaa tcatagccta aaataaataa aatcattgaa acgatcggtg
     1621 ttgtggcttg tgataatgtt cctgtaatca tttcaccaaa ttgcgatgct atcagtaaat
     1681 cataaatccc cgtaaaattc aaaacaagac cgacaatcnn nnnnnnnngt ttcacattct
     1741 ttcttcgcgg tataaacaac tgctaagaat gttcccgata ttatgataag acctatcatc
     1801 acagcaataa caaaagaatt tgtaaaaata cttttaatca tctctttcat cgaagaacct
     1861 gttgccgctt ctttagctat taaaactgga aaaacaataa aagcaataac tgttccagca
     1921 atatcaaaaa taacagtatt actagatttt ccaacaatag aaagatataa cggtaatgcc
     1981 acatttcctc cctctactac tgtaagtaaa taaggtgaaa aatgagcata tttcttgccc
     2041 gcaagtggtg caagtaattt acctgcaact aaagctagaa ggtaaacacc aaaaacataa
     2101 ccaataattt taatatggtc tgtttcaatc gttgctgtac acatcaaatt aaggactaaa
     2161 ataggaaata aaattttaaa tattattgca ttagctccat ttttctgttc aagtgtgatc
     2221 catcccttta ctcgtgcgat aaagcctagt gccagcataa aaaatactgg aaataacgtt
     2281 gttaaagctg tcataataat ctcccttcat ttatataatt tcttctttat gagttaaatt
     2341 ttcccatata ccggaaatat actaaactct ccataatttt taattttatt aatatttttt
     2401 aaaagcatca tatcttcatc actgataata aaatctaaat ccgcattatt ttgcatatgt
     2461 cctggatcag cagttttagg tagtggtaag gtatctaact gtaaacaata cttaatacac
     2521 aattgaggaa ttgtaacccc atatttatca gcaattaatt taacttcatt attttttaac
     2581 atttctccat gacctactgg tgaataagct tcaactaaaa tatcatgtgc tt
//
LOCUS       DAOMTA010000411         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1743_length_2627_cov_2.035381, whole genome shotgun sequence.
ACCESSION   DAOMTA010000411 DAOMTA010000000
VERSION     DAOMTA010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_2627_cov_2.035381"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..382)
                     /locus_tag="PLK68_10640"
     CDS             complement(83..382)
                     /locus_tag="PLK68_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRM90900.1"
                     /translation="MDMIEVSTIDYLPGYRITKSLGIVFSSKIMNQAIASDNTNIEEM
                     LDTARQEAMAETVAQASRRHASAIINVRFTTTNLGNNMIECQVSGMAVKAEKIDE"
     gene            complement(422..2371)
                     /locus_tag="PLK68_10645"
     CDS             complement(422..2371)
                     /locus_tag="PLK68_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90901.1"
                     /translation="MARNFYIDNINDNDKLNEVSLLLNEMEEVSRIKIGKTGISFYCE
                     DPENVQAVLNNHDENLVLKEEINSRKREFVAPEQKVEHIFMFTNLETEEEAKEIEEVI
                     SRYSAYENVSLDFTNKLLKVTTSQKNILVRLNRLVDKVNPKIDVEQWKKPFKSQDIFQ
                     QKYLNTMIRIAGLFVALALGLVTKDDPNFITNIAWLIALIIFNEKTLVQAYKDLKVKQ
                     FLSENITINLACLFGWVYGAYIEAIVVSLIYQVGERLLMRLINLTMEKIDDEINPIQL
                     GRREIGENEYEMVSLEDFDIGDVIVVLPGETIPLGGKIASGESELDMFAINGSDILEP
                     VKAGSEVQSGSVNVKDTLRIKILYTYDRSAMSRVLEIATMAPVGSSRTHRLVELISKI
                     DTVLLVVVGILCATLVPILNFEANFKYIYLGAILITISGSFAYKQASSFAVLSGVAKA
                     FSKNIVIKENSGLDALNLCRTVIYDRFDGVEVTEEEMELFAKLSKLHVGLIIFNDGPV
                     DLENDQYRIYNNLTVDEKLEVMDKASIAGPVAYIGDNSKDIALLQKAYVGISRGGIKD
                     KKVIENSDIMLMNSDLNTVIETFMISKKQKYITIENIFVGLFINVILMILAVLFIIPW
                     WLALVIYLIEVVVVLFNTHRIIDMK"
     gene            complement(2430..>2627)
                     /locus_tag="PLK68_10650"
     CDS             complement(2430..>2627)
                     /locus_tag="PLK68_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90902.1"
                     /translation="SRRRIAFYQRNGLVLNDQHDYFVPPVRNLKHRLYFHLMSYPTEI
                     DALQFERYYPQILQLVYGVNE"
BASE COUNT          903 a          513 c          305 g          906 t
ORIGIN      
        1 tttaagtaaa tttttaacat tctttttatt atagtgattt aataaaaact ccattaacgt
       61 agaatctgtc ttcactaaat acttattcat cgattttttc cgccttaact gccattccag
      121 aaacttgaca ctcaatcata ttgttgccaa ggtttgttgt tgtaaagcga acattaatga
      181 ttgctgaagc atgtcttcga gaagcttgag caactgtttc tgccatcgct tcttgtcttg
      241 ctgtatctaa catttcttca atattagtat tatcactagc aattgcctga ttcatgattt
      301 tactgctaaa aacgattccc agactctttg taattcggta tccaggtaaa taatcaatcg
      361 tacttacttc aatcatatcc attatatttt cctccttata agaaaaggtt actaaagtaa
      421 cctatttcat atcaatgatt cgatgagtat taaataatac aactacaact tctattaaat
      481 aaattactaa tgccagccac caaggaataa taaataaaac agctaagatc attaagatta
      541 cattaataaa caatcctaca aaaatatttt caatcgtaat atatttttgt ttcttagaaa
      601 tcatgaatgt ttcaataacg gtattaagat cagaattcat cagcataata tcactatttt
      661 caatcacttt tttatctttg atgccaccac ggctaatacc tacataagct ttttgcaata
      721 atgcaatatc ttttgaatta tcaccaatat aagcaactgg tccagcaata cttgctttat
      781 ccattacttc taatttttca tcaactgtca aattattata gattcgatac tgatcatttt
      841 caagatcaac tggtccatca ttgaaaataa tcaagccaac atgtaatttt gataatttag
      901 cgaataattc catttcctct tcagttactt caactccatc aaaacgatca taaataactg
      961 ttcgacataa attcaaagca tctaaaccac tattttcttt aataacgata tttttagaaa
     1021 aagctttagc cactcctgat agaacagcaa aagaagaagc ctgcttgtaa gcaaaagaac
     1081 cagaaattgt tattaaaata gcccctaaat aaatatattt aaaatttgct tcaaaattaa
     1141 gaattggcac taatgttgca caaagaattc ctacgacgac taaaagaact gtatcaatct
     1201 tactgattaa ttctaccagc ctatgcgttc ttgaagaacc aacaggtgcc attgtcgcaa
     1261 tttctaagac tctgctcatt gcactgcgat catacgtata taaaatttta attctcaaag
     1321 tgtctttaac attgacactt ccactttgaa cttcacttcc ggctttaact ggctctaaaa
     1381 tatctgatcc attaatggca aacatatcta attcactttc acccgaagca atcttgccac
     1441 ctagaggtat cgtttctcca ggtaaaacta cgatcacatc accaatatca aaatcttcta
     1501 aagaaaccat ctcatattca ttttcaccaa tttctcgacg tcccaattga attgggttga
     1561 tctcgtcatc aattttctcc atcgttaaat tgattaaacg catcaataac cgttctccaa
     1621 cttggtaaat caaagaaacc acgatagcct caatataagc accataaacc catccaaata
     1681 aacaagctag attaattgta atattttcac ttaagaattg tttaactttt aaatctttat
     1741 atgcttgaac caaggtcttt tcattaaaaa taatcaaagc aatcagccag gcaatattag
     1801 ttataaaatt aggatcatct tttgtaacta atccaagtgc taaagctaca aataatcctg
     1861 caattctaat cattgtattt aaatattttt gttgaaaaat atcttgtgat ttaaatggct
     1921 tcttccattg ctccacatca attttaggat taactttatc aactagacga tttaatcgca
     1981 ctaaaatatt cttttgacta gttgtgactt taagtaactt attagtaaaa tcaagactaa
     2041 cattttcata ggctgaatat cgtgaaataa cttcctcaat ttcttttgcc tcttcttctg
     2101 tctctaaatt agtaaacata aaaatatgtt ctactttttg ttctggtgcc acgaattcac
     2161 gcttacgtga gttgatttcc tcttttaaaa caagattttc atcatgatta ttcaaaaccg
     2221 cctgcacatt ttcaggatct tcacaataaa atgaaatacc tgtttttcca atcttgattc
     2281 gtgaaacttc ttccatttcg tttaagagga ggctgacctc atttaattta tcattatcat
     2341 ttatattatc aatatagaaa ttacgagcca tagtaatctc ctttccaata gtcacctaat
     2401 tttagcatat cttcaaaaaa attccaactc tactcattaa caccataaac taactgtaaa
     2461 atttgaggat aataacgttc aaattgcaat gcatcaattt ccgttggata agacattaaa
     2521 tgaaaataca gacgatgttt taagttacgc acaggtggta caaaataatc atgctgatca
     2581 tttagcacta agccatttct ttgataaaac gcaatccgtc gacgact
//
LOCUS       DAOMTA010000412         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1755_length_2617_cov_2.359094, whole genome shotgun sequence.
ACCESSION   DAOMTA010000412 DAOMTA010000000
VERSION     DAOMTA010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_2617_cov_2.359094"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLK68_10655"
     CDS             complement(<1..510)
                     /locus_tag="PLK68_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HRM90903.1"
                     /translation="MQTYLLSRKYFKLAFIIYAFINTLALGFRLNILLFIMIGWGGLI
                     IGWDFYQKQINYHDIKFILIITYGLILFLATAINPYSNLKSYLIALLQLMIFFLIYGN
                     QKSFSSNNIYDELKSIIPLTNLLTGLAGICSLIMYLFKFSKIQNGWPIGLVGNRLFGV
                     YFNCNPAAFL"
     gene            658..>1040
                     /locus_tag="PLK68_10660"
     CDS             658..>1040
                     /locus_tag="PLK68_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRM90904.1"
                     /translation="MKNITLVIMAAGIGSRFGGGIKQLAPVGPNGEIIMDYSIYDAKK
                     AGFNKVVFVIRKDLEAEFDAVIGSRIKQIIDVEYVFQELDNIPDEYQETFKQRTKPWG
                     TGQAILCCKDVINEPFLVINADDYYG"
     assembly_gap    1041..1053
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1054..1584
                     /locus_tag="PLK68_10665"
     CDS             <1054..1584
                     /locus_tag="PLK68_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRM90905.1"
                     /translation="QAYEEAYKYLNENHDNTAKQQIAMVSFVLKNTLSDNGGVTRGVC
                     TVDQNNHLCDIVETHNIEKGDNCAFVKKENQIIELDLEVPVSMNMWALQPEIFDILDS
                     KFKLFLSQLENDNFKDEYLLPTIIGSLLKENQVEVTVLKSLDNWFGVTYKEDKQTVIE
                     SIQKLTAVGVYPQKLF"
     gene            1683..>2617
                     /gene="mgtE"
                     /locus_tag="PLK68_10670"
     CDS             1683..>2617
                     /gene="mgtE"
                     /locus_tag="PLK68_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRM90906.1"
                     /translation="MEQIKLIEKLLEIRKYKEIKEILKEMNDVDVAEMLEGFSDENMI
                     RIFRLLPKDDAADIFAYMSSDREHALIDSLTNKELENIINDLYSDDVMELLEELPANV
                     VKRIIAASNPETRRDINHLLRYPEDSAGSNMNIDFVDLRADMTVKEAIARIRRIGVDK
                     ETINTCYVIDNFRHLLGIVTLRKLVLSSQSALIEEIMNDNLITVHTMEDQEDVAHDFQ
                     KYDLTSMPVVDNENRLVGIITVDDIVDIMQEETTEDIEKMAAMVPSDKPYIKNGPFET
                     FKKRIPWLLLLMISASITGKIIQGFEHALAGSVILT"
BASE COUNT          953 a          322 c          480 g          849 t
ORIGIN      
        1 caaaaaggct gcaggattac aattaaaata tacaccaaat aatcgatttc ctactaatcc
       61 gattggccat ccattttgaa ttttcgagaa tttaaataaa tacattatta aagaacagat
      121 tccagctaga ccggttaata ggttggtcag aggaataata ctttttaatt catcgtagat
      181 attgtttgat gaaaaacttt tttgattccc gtagattaaa aaaaagatca ttagctgtaa
      241 aagtgcaatt aaatatgatt ttaaattact atatggattg atcgctgttg caaggaacaa
      301 aattaggcca taggtaataa ttaaaataaa tttaatatca tggtaattaa tttgtttttg
      361 ataaaagtcc catccaatga taagtcctcc ccatccaatc atgataaata acaaaatatt
      421 aagtcggaaa cctagggcaa gtgtattgat aaaagcataa ataatgaatg ctaatttaaa
      481 atattttctt gataatagat aagtctgcat aatccccctc catgatattc attctaacaa
      541 aatattgata ttaatactac tttaatttgt ttgctgcgat ttgcttatta caataactat
      601 taaaatagtt aaagatagtg tataattaat gaagaatatt tagaaatgag ggactttatg
      661 aagaatatta cattggttat tatggcggcc ggaattggta gtcgttttgg aggtggcatt
      721 aaacaattgg caccagtagg accaaatggt gaaataatca tggattattc catttatgat
      781 gctaaaaagg caggttttaa taaagtagtt ttcgttattc gtaaggattt agaagcagaa
      841 tttgatgctg tgattggatc acgaatcaaa caaattattg atgttgaata tgtttttcaa
      901 gaactagata atattcctga cgaataccaa gaaacattta aacaaagaac aaaaccatgg
      961 ggaacagggc aggcaatttt atgttgcaag gatgtaatta atgaaccatt tttagttatt
     1021 aatgctgatg actattatgg nnnnnnnnnn nnncaggcat acgaagaagc ttataaatac
     1081 ttaaatgaaa atcatgataa tacggctaaa caacagattg cgatggttag ttttgtactt
     1141 aaaaatactt tgagtgataa tggtggtgta acacgtgggg tgtgcacggt tgatcaaaac
     1201 aatcatcttt gtgatattgt tgagacacat aatattgaaa aaggtgataa ttgtgcattt
     1261 gttaaaaaag aaaatcaaat tattgaactt gatctcgagg taccggtatc aatgaatatg
     1321 tgggctctac aaccagaaat atttgatatt ttagattcta agttcaaatt gtttttatct
     1381 caattagaga atgataattt taaagatgag tatctattac caactattat aggttcacta
     1441 ttaaaagaaa atcaggttga agtgactgtg ttaaaatcgt tagataattg gtttggagtt
     1501 acatataaag aagataaaca aacagttatt gaaagtattc aaaaattaac agctgtcggt
     1561 gtttatccac aaaaattatt ttaatagaaa ccgaaggaag atgtgggtat cttccttttg
     1621 tctttcatca gcatatactc tttggtataa ttaataatga tatgaaaaac ggggaaagtc
     1681 gaatggaaca aattaaatta attgaaaaat tattagaaat aaggaaatat aaggaaatta
     1741 aagaaatctt aaaagaaatg aacgatgtgg atgttgccga aatgctcgaa ggtttttctg
     1801 atgaaaatat gatacgtata tttagattat tgccaaaaga tgatgctgca gatatttttg
     1861 catatatgtc aagcgacaga gaacatgcgt taatagattc gttaaccaat aaagagttag
     1921 agaatattat taatgatctt tattcagatg acgtaatgga acttcttgaa gagttacccg
     1981 ctaatgttgt gaagcgaatt attgctgctt ccaatcctga gactcgtcgt gatataaatc
     2041 atttattacg ctatcctgaa gattctgcag gtagtaatat gaatatcgat tttgttgatt
     2101 tacgtgcaga tatgacagtt aaggaagcga ttgcacgaat tagacgtatt ggagtagata
     2161 aagaaacaat caatacctgt tatgtaatcg ataattttcg tcatcttttg ggaattgtta
     2221 ctttgagaaa gttagtttta tcatcacaaa gtgctttgat tgaggagatt atgaacgata
     2281 atcttattac agttcatacg atggaggatc aagaggatgt agcacatgat tttcaaaaat
     2341 atgacttaac ttcaatgcct gtggttgata atgaaaatcg tttagttgga atcattacag
     2401 ttgatgatat tgttgatatc atgcaggaag aaacaactga agatatcgaa aaaatggccg
     2461 caatggtacc gtcagacaag ccttatatta aaaatggccc gtttgaaaca tttaaaaaaa
     2521 gaataccgtg gcttttgtta ttaatgattt cagcttctat tactggaaaa atcattcaag
     2581 gatttgagca tgctttagcg ggctctgtga ttttaac
//
LOCUS       DAOMTA010000413         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1756_length_2617_cov_2.055816, whole genome shotgun sequence.
ACCESSION   DAOMTA010000413 DAOMTA010000000
VERSION     DAOMTA010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_2617_cov_2.055816"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1533)
                     /locus_tag="PLK68_10675"
     CDS             complement(124..1533)
                     /locus_tag="PLK68_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM90907.1"
                     /translation="MSREKTLVKNTLIMALGTFLPRIINLLTTPIITSATTDAQYGQL
                     DLVTTTILSFIVPLCTLQIEQALFRFLVDAKSEKEQRRVITNGYVMIFGLMVIAAIIC
                     LFVPISMFEGSFKLLIIGYIWIEIIATTSRFVLRAFSKYKEYSILAALVVIVNFVVVS
                     VCLLWLKTGYIGVLIALVAADVVGFIYVLCVCNIFKFFNFKYFSSEYALKMLGYALPF
                     VPNMVSWYINQLSDRWIISIFLGAGPNGVYALANKIPSIVNILYPAFNLAWTDSATRS
                     VNDPDSGRYYNRMFRMLFCIVSAGSVLLVAGSPIIFGILCRNKELYSAFDYTPTLILA
                     TYFYCFSQFFGSIYVAVKSAKNMSVTTTIAAIINIIINLGLINIIGVQAAVLSTLAAN
                     LFLAGYRFFDLNRRYVRLKINKRLTVLTIVIFIISMSLAWSGDKILWILNIVIALGYA
                     YFISGDIFIGMFKAVLKKR"
     gene            complement(1577..2107)
                     /locus_tag="PLK68_10680"
     CDS             complement(1577..2107)
                     /locus_tag="PLK68_10680"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRM90908.1"
                     /translation="MDKKVLRKQLIQARLDLDSETYASKSNFIVSKLKQQPEFIEARA
                     IGIYVSFRHEVETISLIKEIINNKIVCVPKISGKQMDFYQINSINELKTSNFGILEPN
                     NSHPVTKDNLDLLIVPMVGYDQSGNRLGYGGGYYDRYLSDYCGNVIGLAFSFQEVANL
                     PVEPFDLPIKKIINEK"
     gene            complement(2100..>2617)
                     /locus_tag="PLK68_10685"
     CDS             complement(2100..>2617)
                     /locus_tag="PLK68_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM90909.1"
                     /translation="TMKYPTCVLMEEGAKGSCITIAVAGENQIMDSGSKMIHLAPNTS
                     SSIVSKSVSRRGGKVNYRGMVQHGAKAYNSKSKVECDTLILDDISTSDTVPVNWMRNN
                     DSIIEHEATVSKISEEQLFYLMSRGLTKKEAMEMIVMGFIEPFARELPMEYAVELNQL
                     IKLDFGDQGIG"
BASE COUNT          962 a          523 c          319 g          813 t
ORIGIN      
        1 atatgcttcc atattatcct cctgtaaccc aattcattaa tattaactat acaaaaaagg
       61 acataaaaaa aatgttaaaa tttaattact ttgccaaata attaaatctt aacattatta
      121 ctattaacgt ttctttaaaa cagctttaaa cattccaata aaaatatcac cagaaataaa
      181 gtatgcgtag ccaagcgcaa taacaatatt taatatccat aaaattttat ctcccgacca
      241 tgctaaagac atactaatta taaaaattac gattgtcaac actgttagac gtttatttat
      301 ttttaaacgt acatagcgcc gattcaaatc aaagaaccga tatcccgcta aaaataaatt
      361 cgcagccaat gttgaaagaa cagctgcctg tactccaata atattaatca aacctaaatt
      421 gatgataata ttgattatag ctgcaatagt cgttgttacc gacatatttt tagcactttt
      481 aacagctaca taaatacttc caaaaaattg tgagaagcaa taaaaatatg tagctaaaat
      541 taaggttggg gtataatcga aagcactata taattcttta ttacggcata aaattccaaa
      601 gatgatcggt gatccagcca ctaataaaac tgaacctgct gagacaatgc aaaacaacat
      661 ccgaaacatc ctattataat acctgccact atcaggatca tttacactcc ttgttgccga
      721 atctgtccaa gctaaattaa aggccggata taagatatta acaatcgaag gtattttatt
      781 agccaaggca taaactccat ttggccctgc ccctagaaaa attgaaataa tccatcgatc
      841 agacaattga ttaatatacc atgataccat attaggtaca aacggtaatg catagccaag
      901 cattttaaga gcatattcgc tactaaaata tttaaaatta aaaaatttaa aaatattaca
      961 tacgcatagt acataaataa agccgactac atcagcagct actaatgcta ttaaaacccc
     1021 aatataccct gtctttagcc acaataaaca aactgagaca acaacaaagt tgactattac
     1081 aactaacgcc gccaaaatag agtactcttt atatttggaa aaagccctta aaacaaacct
     1141 agaagttgtt gcaataatct caatccatat atatccaata atcaatagct taaaactgcc
     1201 ttcaaacatg ctgattggca caaacagaca aataatcgca gcaatcacca taagtccaaa
     1261 aatcattaca tatccgttag taatgacacg ccgttgttct ttttcacttt tagcatcaac
     1321 aagaaatcta aatagagcct gttcaatttg caacgtacat aaaggtacaa taaatgaaag
     1381 aatcgtcgtt gtaactaaat ctaattgccc atactgcgca tcagtagttg cactagtaat
     1441 aattggcgta gtcaataaat taataatccg aggcagaaaa gtccccaacg ccataattaa
     1501 cgtattttta actaatgttt tctctcttga cataatttca ccctacttta tattccccat
     1561 tataacaaaa ccgtgattat ttttcattaa taatcttttt aatcggcaaa tcaaatggtt
     1621 ctactggtaa atttgcaact tcttggaaac taaatgccaa gccaattaca ttaccacaat
     1681 aatcacttaa atagcggtca taataaccac cgccataacc taatcgatta ccactttgat
     1741 cgtagccaac cattggtaca attagtaaat caagattatc tttagtaaca ggatgactat
     1801 tattaggttc taaaattcca aaattacttg tctttaattc attgattgaa ttaatttgat
     1861 aaaaatccat ctgtttacca ctaatttttg gaacacagac aattttatta ttaattattt
     1921 ccttaatcaa actgattgtt tctacttcat gtctaaaaga aacatagatt ccaattgctc
     1981 ttgcctcaat aaattcaggt tgctgtttta atttagaaac aataaaatta gatttagatg
     2041 cataagtttc gctatctaaa tctaatcttg cttgaatcaa ttgtttccgt aatacttttt
     2101 tatccaatac cctgatcccc aaaatccaat ttaattaatt gatttagttc aacagcatat
     2161 tccattggta attcgcgagc aaacggctca atgaacccca ttacaatcat ttccattgct
     2221 tctttttttg ttaaaccacg agacattaag tagaaaagct gttcttcaga aatttttgag
     2281 acagtcgcct catgctcaat aatagaatca ttatttcgca tccaattaac cggaactgta
     2341 tcactagttg aaatgtcatc taaaatcaac gtatcacatt caaccttgct ttttgaatta
     2401 taagctttag caccatgctg taccattccc cggtaattga cttttccacc gcggcgagaa
     2461 actgatttag aaacaatact acttgatgta ttaggcgcta agtgaatcat ctttgaacct
     2521 gaatccataa tctgattctc acccgctaca gcaatagtaa tacaagaacc ttttgctccc
     2581 tcttccatca acacacaagt tggatacttc atagtta
//
LOCUS       DAOMTA010000414         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1757_length_2617_cov_1.823966, whole genome shotgun sequence.
ACCESSION   DAOMTA010000414 DAOMTA010000000
VERSION     DAOMTA010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1757_length_2617_cov_1.823966"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1421
                     /locus_tag="PLK68_10690"
     CDS             240..1421
                     /locus_tag="PLK68_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HRM90910.1"
                     /translation="MENILVIGNGFDIAHGFNTRYEDFINFCKTIATVYQGFKDNQFI
                     YQNEYNDILKEKLDKVFKEKSVSAKAIKYFSNLTPTSETSQFIKACKENYWLTYVLKN
                     KSMIGDKWSDLEYVIAKQIEILSYISNNLNWHTREETMLASRYSSNLIELFKIVTEER
                     GNNTDFQHQIELTKKHLYRELEELTWLLEIYLTRFLNTKTKKIELFKYLPVTKLISFN
                     YTDTYTRMYKKETNTVHYIHGFAAKDRIKEENNMVFGIGSEIKNVTDNDKYDYLEFQK
                     YYQRIVKKTGNNYTKWLNDNEQFYIYFFGHSLDIVDGDVIRKLIHCKKAKVIIFYYNQ
                     KALNALVVNLAKILGKDELIQFTNEEKITFYKSDDLEIIKKLKNTMYQTRHEKLKSTV
                     R"
     gene            1447..1872
                     /locus_tag="PLK68_10695"
     CDS             1447..1872
                     /locus_tag="PLK68_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM90911.1"
                     /translation="MEKIIKVGIGVLLIKNNQILLGHRIKDGVDTGGIYEPDTWCLPG
                     GKQEYHETIFEGAIREVKEETNLNISQIEVFNVVDDIQLNKHYVTIHIIAKNYDGDLK
                     AMEPDKQDEWCWFEIEKLPNNIYSPSKKFIEAYLDRSIT"
     gene            2122..2307
                     /locus_tag="PLK68_10700"
                     /pseudo
     CDS             2122..2307
                     /locus_tag="PLK68_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT         1056 a          266 c          421 g          874 t
ORIGIN      
        1 gactctaaat caatatgcct aatcaaatta atattaatga tagaacaaaa gaattcaaaa
       61 taagaagata tttattttat ttttattgca atttttttat tatcttatca ttgttaaagg
      121 tgaaaatgcc tagcactgag tctatctgtt tataaaggtt tatttaatag ttgacagtaa
      181 aaaatagtta gataataatt agtaaattaa aattttgatt gataaggggg agtatggtga
      241 tggagaatat attagtaatt ggtaacggct ttgatattgc acatggtttt aatactcgtt
      301 acgaagattt tataaatttt tgtaagacta ttgcaacggt ttatcaagga tttaaggata
      361 atcaattcat atatcaaaat gaatataatg atatattaaa agaaaaatta gataaggttt
      421 ttaaagaaaa gtcggtttct gccaaagcaa taaaatattt ttcaaactta actccaacat
      481 ctgaaacttc tcaatttatc aaagcatgta aagaaaatta ttggcttaca tatgtcttaa
      541 aaaataaaag tatgattggg gataagtggt cagatcttga atatgtcatt gctaaacaaa
      601 tagagatatt atcatatatt tcaaataatc ttaactggca tactcgagaa gaaactatgc
      661 tcgcttcacg atattctagt aatttaattg aattatttaa gattgtaaca gaagaacgtg
      721 gaaataatac cgattttcag catcagatag aactcacaaa gaaacattta tatcgagaat
      781 tggaagaatt aacatggtta ttagaaatat atttaacacg atttttaaat actaaaacca
      841 agaaaattga attatttaaa tatttaccag taactaaact catttctttt aattatactg
      901 atacgtacac aagaatgtat aaaaaagaaa caaatacagt tcattatatt catggatttg
      961 ctgctaaaga taggataaaa gaagaaaata atatggtttt tggaattggc tcagaaatta
     1021 aaaatgtcac cgataatgat aagtatgatt atcttgaatt tcaaaagtat tatcaaagga
     1081 ttgttaaaaa aacaggtaat aactacacta aatggttaaa tgacaatgaa caattttata
     1141 tctatttttt tggtcattcc ttagatattg tagatggaga tgtaattaga aaactgattc
     1201 attgtaaaaa agcaaaagta attatctttt attataatca aaaagccttg aatgcattag
     1261 ttgttaattt agcaaagatt ttaggaaaag atgaacttat tcaattcact aatgaagaaa
     1321 aaatcacttt ttataaatct gatgatcttg aaataattaa aaaacttaaa aatacaatgt
     1381 atcaaactcg tcatgagaag cttaagagta cagttagata aatccaatta gaaatgagga
     1441 aataatatgg agaaaatcat taaagtggga ataggtgttt tgttaatcaa gaataatcag
     1501 attcttttag gacatcggat aaaagatggg gttgatactg gtggcatcta tgaaccggac
     1561 acatggtgtc taccaggtgg aaaacaagag tatcatgaaa ctatttttga aggagcgatt
     1621 agagaagtta aagaagaaac taatttaaat atctcacaga tagaagtttt taatgtagtc
     1681 gatgatattc aattaaataa gcactatgta acgatacata ttattgctaa aaattacgat
     1741 ggagatttaa aagctatgga accagataaa caagatgagt ggtgctggtt tgaaatagaa
     1801 aagctgccta ataatattta ttccccttca aaaaaattta ttgaagcata tttagataga
     1861 agtatcacct agataaatct cattataaga acgatatttt tgaaatcatt ggcgttgaag
     1921 atagactaaa tacattatta acaatgtggt taacacgtgt ttatactatt aaaggaaata
     1981 ttatgaataa atatacaatg ataaaagaag ttatttgaaa aacaacaaga tattaaaaaa
     2041 gcagataaaa cgtcaaaata tatgagagat ttatttacat tttatggaat tccctcaccc
     2101 tagcgcaaag ataagacaaa tacaaacttg ctggagcaaa aaattgttaa tagttttgga
     2161 agtgatgagt tttttattaa taaagcaatt ggctggagtt taagaaatta ttttagaact
     2221 catttggtat gggtaataaa ctttattatt aaatatcgta ctctaatgaa taagctaagt
     2281 attaaagaag ctagtaaata tttataattt atagtgttgc atgtattttt ttgatattta
     2341 agatggatat aattgataat atacaaagtt attataagaa tatattttat gaaagagtat
     2401 ttcgcctgta ggtatgattt gtcaatagac tgtttattct gagagtctga caaatcttaa
     2461 agattgagac gtatatgagt ctgttatttt atatagaaaa ttttgtagtt gtaggaagga
     2521 catatccttt aatatatacc gaggaaaagg aaaaaggcaa gacattattt attgaaatac
     2581 tattaattgg agttggactc gcaatggata cattgtt
//
LOCUS       DAOMTA010000415         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1758_length_2616_cov_2.043733, whole genome shotgun sequence.
ACCESSION   DAOMTA010000415 DAOMTA010000000
VERSION     DAOMTA010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_2616_cov_2.043733"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="argB"
                     /locus_tag="PLK68_10705"
     CDS             <1..636
                     /gene="argB"
                     /locus_tag="PLK68_10705"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRM90912.1"
                     /translation="DIKSEFKNGLRVTDKETLEVAEMVLSGKINKGLVQHMERIGTHA
                     VGLSGKDGNMITVKKAMPKGDDIGYVGEIINVDVTLLETLLEKDYTPIISTVGLDSKY
                     NAFNINADDVATGVARALKASKLVFLTDIEGVLRNPSDPSTRISVINTESAAELFEQG
                     IITGGMIPKLKNCLEAVQEDVKKVHILDGRLEHSLLIEIFTTSGVGTEIKK"
     gene            complement(650..1468)
                     /locus_tag="PLK68_10710"
     CDS             complement(650..1468)
                     /locus_tag="PLK68_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HRM90913.1"
                     /translation="MRKKIRTIKLLLLIIVIIGSGFGYYYSRYIAPDSYSIKKTTITN
                     NSLPDEFKNFEIGFISDINLQKSSDVTRLKKIVTSLNKENVDMVIFGGDLFSATPFDN
                     DKVIELLSNIKSKYGKFAVLGEKDLASSNDVNAILNEGGFEVLHNEYRPIYFNGSTIS
                     LFGLEGTGELSGLINENNSESYKIVAVHEPDYFNTTSNNEIALQLSGHTMGGYIRLPF
                     IGGLFKKTNGNTYVSGEHTKSKSQLLISNGLGMEDGYEYRLFCPNQINIVTLKK"
     gene            complement(1465..>2616)
                     /locus_tag="PLK68_10715"
     CDS             complement(1465..>2616)
                     /locus_tag="PLK68_10715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM90914.1"
                     /translation="PADYQKMSFLGETGVTLLMSDSTNSEVPTFSISEKKVANSVQEE
                     FRKTEGRLIVATFASNVHRVQQIVEAAVKFNRKILVYGRSMENNIQVSRAMGYIKCPD
                     KFFIKSEQAKKLPDNEILILCTGSQGEALAALSRIANGTHKYVKIKPGDTVVFSSNPI
                     PGNAYSVNVVVNKLFRAGAKVLTNTAFNNLHTSGHASQEEQKLMMLLTRPKYFFPVHG
                     EYRMLKIHAELSQDVGIPPENSFVLSNGDTILLNNGEARLGPRIHVEDIYVDGNDISG
                     LSTAVLRDRQMLSEDGMVSILISMDSRSGKLLTRPVIISRGFVYMKDSFHMIREAEKL
                     VETNLSKLLQGKTTFGEIKNTTRDTLSSYFYRKTKRNPMIIPVIMNKKS"
BASE COUNT          891 a          418 c          430 g          877 t
ORIGIN      
        1 gatattaaaa gtgaatttaa aaatggttta cgggtaactg ataaagaaac tttagaagtt
       61 gctgagatgg ttttgtcagg gaagatcaat aaaggattag ttcaacatat ggaaagaata
      121 ggaacccatg cagttggctt atctggtaaa gatggtaata tgattacagt aaaaaaagca
      181 atgccaaaag gggatgacat tggttatgtt ggggaaatta taaatgttga tgttacttta
      241 ctagaaacat tgttagaaaa ggattataca ccgattattt caacagtagg attagattct
      301 aagtataatg cttttaatat taatgcagat gatgtagcaa cgggtgtagc tcgtgcttta
      361 aaagcgagta agttagtatt tttaactgat attgagggag ttttacgtaa tccgagcgat
      421 cctagtacac gcatatcagt aattaataca gagtcagcag ctgaattatt tgaacaagga
      481 attataaccg ggggaatgat tccaaaatta aaaaattgtc ttgaagcagt tcaggaggac
      541 gtaaagaaag tccatatttt agatggccgt ctggaacata gtttattaat tgaaattttt
      601 acaactagcg gggttgggac agaaataaaa aaataaaggt ttagagcctt tattttttta
      661 atgtcacaat attgatttga ttaggacaga aaagacgata ttcgtatccg tcttccatac
      721 ctaaaccgtt agaaatcaat aattgacttt ttgatttagt gtgttcacca ctaacataag
      781 tattaccgtt agttttttta aatagtccgc caataaaagg aagacgaata taaccaccca
      841 ttgtatgacc ggaaagttgt aaagcaattt cattatttga agtcgtgtta aaataatctg
      901 gttcatgaac tgcaactatt ttgtaacttt cactattatt ttcattaatt agaccactta
      961 attctcctgt tccctcaaga ccaaaaagac taatcgtaga tccattaaaa taaataggac
     1021 gatattcgtt atgcagaact tcaaaaccac cctcatttaa aatggcattg acatcatttg
     1081 atgaagctaa atctttttca ccaagaacag caaatttacc atatttagat tttatattgg
     1141 ataataattc gattacctta tcgttatcaa agggcgtggc tgaaaagagg tcaccaccaa
     1201 aaataaccat atcaacattt tctttgttta atgaagtgac aatttttttt aatctagtaa
     1261 catcactact tttttggaga ttaatatcgc tgataaaacc aatttcaaaa ttcttgaatt
     1321 catcaggtaa actattatta gtaatagtcg ttttttttat agaataacta tcaggagcga
     1381 tatatcggct ataataataa ccaaaaccgc tcccgataat aacgattatc aataatagta
     1441 gtttaatcgt tcttattttt tttctcatga ttttttattc atgataaccg gaataatcat
     1501 tggattccgt ttagttttgc gataaaaata tgaagataat gtatcgcggg tagtattctt
     1561 aatttctccg aaagtagttt taccttgtaa caattttgat aaatttgttt caactaactt
     1621 ctcagcttca cgaatcatgt gaaaactgtc tttcatatat acgaaaccac gtgagataat
     1681 tactggtctt gtcaataatt ttccactacg agaatccata ctaattaaaa ttgaaaccat
     1741 tccatcttca gataacattt gtcgatcgcg aagtacagct gtggataagc cgctaatatc
     1801 attaccatca acataaatat cttcaacatg aattctcgga cctaatcgag cttcaccatt
     1861 attcaataaa attgtatctc catttgataa aacaaatgaa ttttctggtg gaataccgac
     1921 atcttgagat aattcagcat gaatcttcaa catgcgatat tctccatgga cagggaagaa
     1981 gtatttaggt cttgtcaata acatcatcag tttttgttcc tcttgagaag cgtgtccgga
     2041 agtatgtaaa ttattgaaag cggtatttgt tagtacctta gcaccagctc taaataattt
     2101 atttactact acattaacac tataagcgtt acctggaatt ggatttgatg aaaagacaac
     2161 agtatcgccg ggtttgattt tgacatattt atgagttcca tttgcaattc gacttaatgc
     2221 tgctaatgct tcaccttgag aaccggtaca taaaatcaaa atttcattat ctggtaattt
     2281 ttttgcttgt tcacttttaa taaagaactt atcaggacat ttaatatagc ccatagctct
     2341 tgaaacttga atgttgtttt ccattgaacg accatatact aaaattttac gattaaattt
     2401 aacagctgct tcgacaattt gttgtactcg atgaacgttg gaagcgaaag ttgctacaat
     2461 caaacgtccc tctgtttttc taaattcctc ttgaacacta ttagcgacct tcttttcact
     2521 gattgaaaaa gtaggaactt cagaattagt agaatcactc attaataaag taaccccagt
     2581 ttcacctagg aaagacatct tttgataatc tgctgg
//
LOCUS       DAOMTA010000416         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1767_length_2612_cov_2.260070, whole genome shotgun sequence.
ACCESSION   DAOMTA010000416 DAOMTA010000000
VERSION     DAOMTA010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1767_length_2612_cov_2.260070"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLK68_10720"
     CDS             complement(<1..333)
                     /locus_tag="PLK68_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM90915.1"
                     /translation="MDSYLNMLVSLIDFRSYFTVTHTITTCTISLELARLLSLSSEQT
                     KKIYYGAMMHDLGKIGIPIEILEYPGQLTQEQMIIMRSHVLKTRELLEEKIDQEILEI
                     ACRHHEKLN"
     gene            complement(334..666)
                     /locus_tag="PLK68_10725"
     CDS             complement(334..666)
                     /locus_tag="PLK68_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90916.1"
                     /translation="MFIKNFSPLDKLAPAILYHHLDYQKMIYIDDEIKNISQIINLCD
                     RIDVFLQTSNDYSMLKDYLDKESGTRFVENLVTTLYWQVEQVYQFTTNPFSKWQKVTS
                     LNISLSLE"
     gene            complement(657..884)
                     /locus_tag="PLK68_10730"
     CDS             complement(657..884)
                     /locus_tag="PLK68_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90917.1"
                     /translation="MNYVDSRLIDHGLRVANMTYRMLEAQNSHEQKYLQDVFMLAMLH
                     DIGAYKTEKLIKCLFLRQKIYGNMLFMVICS"
     gene            complement(951..2012)
                     /gene="eutH"
                     /locus_tag="PLK68_10735"
     CDS             complement(951..2012)
                     /gene="eutH"
                     /locus_tag="PLK68_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HRM90918.1"
                     /translation="MNFFILIMLILCLIGLIDKILNNKLGLVAAFDKGMDSMGGIAMS
                     MIGFYCIAITLIQNNVDVITKISADSSLDPSIVVGSILAPDMGGFSIVSGLGNSTFLI
                     FSGVILTATLGQTISFQLPIFLASLKKEDLNPFISGLVYGILSLPIILVAVAWYLQIP
                     NLLINLLPVIALYVSYDKTIFVLTLFGYLIRIISILLFGMVVLQLFFDTLPFTTTALI
                     SDAMVIVLRMCIVVCGSMILSDLIIKRFSRMIFMIGQKLGVNSASVMGLLLSLGTSIA
                     MIPLFSQMDRKGKMMNAAFSVSGAYVLGGQLGFISSVVDGNGVIIYMISKIIAGLLAI
                     VFVLIFYREEKVSVDAMEK"
     gene            complement(2086..>2612)
                     /locus_tag="PLK68_10740"
     CDS             complement(2086..>2612)
                     /locus_tag="PLK68_10740"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610580.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRM90919.1"
                     /translation="LAENEVCQRLPGTDGKTKMSKSIGNCIYLADTSEDVRKKVMSMY
                     TDPNHLKVEDPGTVEGNTVFTYLDCFCKDEYFEKYLPDYKNLDELKEHYRRGGLGDVK
                     IKKFLFNVLEETLNPIRERRAVLQADIAAVMKVLEDGCVKANEKANATLKRAKDAMKI
                     NYFDDKDNITEYFK"
BASE COUNT          951 a          516 c          279 g          866 t
ORIGIN      
        1 attcagtttt tcatgatgcc gacacgcaat ttctaaaatt tcttgatcta ttttttcttc
       61 taataattct cttgttttaa gaacatgact acgcattatt atcatttgct cttgagtcag
      121 ttgacccgga tattctaata tttcaattgg aattccaatt ttaccaagat cgtgcatcat
      181 tgcaccataa tagatctttt ttgtctgttc agaacttaag cttaataatc ttgctaactc
      241 taaagatatc gtgcatgtgg taatagtatg tgtaactgta aaataactac gaaaatcaat
      301 tagagaaact aacatattta aataactatc caactattct aatgaaagcg aaatatttaa
      361 actagttact ttttgccatt ttgaaaatgg attagttgta aattgataga cttgctcaac
      421 ctgccaataa agagtagtaa ccaaattttc tacaaatcta gttcctgact ccttatctaa
      481 gtaatcttta agcatgctat aatcatttga agtttgtaaa aatacatcaa ttcgatcaca
      541 caaattaata atctgagaga tatttttaat ttcgtcatca atataaatca ttttttgata
      601 atcaagatga tgataaagaa tagcaggtgc caatttatct agtggtgaaa agttctttat
      661 gaacaaataa ccataaatag catgttgcca tatatttttt gtctcaaaaa taaacatttg
      721 atcaattttt ctgtcttata tgctccaata tcatgtagca ttgctaacat aaaaacatct
      781 tgcaagtact tttgttcatg actattttga gcctcaagca tacgatatgt catattagct
      841 actcgtaaac catgatcaat caatcgcgaa tcaacataat tcaaacaatt acttataatt
      901 tcaataaaac cttaaaacct tcattattta ctcctgcaat attattcaac ttatttttcc
      961 atcgcatcca ctgacacttt ttcctctcga taaaatatca atacaaaaac aattgccagt
     1021 aaacctgcga ttatcttact aatcatatag attatgacgc cattaccatc gactacacta
     1081 gaaatgaatc ctagctgtcc ccctaaaaca taagctccac ttacactaaa agcggcattc
     1141 atcatcttcc ctttacgatc catttgtgaa aacaatggaa tcattgcaat gcttgtccca
     1201 aggcttaata gtagtcccat cacactagca ctattgacac ccaacttttg accaatcatg
     1261 aaaatcattc gtgaaaaacg tttaataatc aaatcgctta aaatcattga tccgcacaca
     1321 acaatgcaca tccgtaaaac aattaccatt gcatcactaa ttaaagcagt tgtagtaaaa
     1381 ggcaatgtat caaaaaacag ctgtaaaaca accattccaa acaacaagat actaataatt
     1441 ctaatcaaat aaccaaataa tgttaaaaca aaaatggttt tatcataaga aacatataga
     1501 gcaattaccg gtaataagtt aattaataaa ttaggaattt gtaaatacca ggcaactgcc
     1561 actagaatta ttggtaaact taaaatccca taaactaatc cactaataaa tggatttaaa
     1621 tcttcctttt ttaaagatgc taaaaaaatt ggtaattgaa aactaattgt ttgtcctaat
     1681 gttgcagtca aaataacccc tgaaaaaatc aaaaaagtac tattaccaag tcccgaaaca
     1741 attgaaaacc cacccatatc aggtgcaaga atactcccaa caacaattga tggatctaat
     1801 gatgaatcgg cacttatttt agtaatcaca tctacattat tttgaatcaa tgtaattgcg
     1861 atacaataaa acccaatcat actcatggca attccaccca tcgaatccat ccctttatca
     1921 aaagcagcta ctagtcccaa cttattattc aagatcttgt caatcaaacc aatcaagcac
     1981 aaaataagca ttattaatat aaaaaaattc attatcgtac ctcaatttcc atattcttcc
     2041 aatattttac caaacaaacc tcaaacaaaa aagcctattc ggcttttatt taaaatattc
     2101 agtaatatta tccttatcat caaaataatt gatcttcatt gcatctttag cacgtttaag
     2161 tgttgcattc gctttttcat tagctttaac acaaccatct tctaacactt tcataacagc
     2221 agcaatatca gcttgtaaaa cagctcttct ttctctaatt ggatttaatg tttcttctaa
     2281 aacattaaat aaaaattttt ttattttaac atcacctaaa ccaccacgac gataatgctc
     2341 ttttaattca tcaagatttt tataatcagg taaatatttt tcaaaatatt catctttgca
     2401 aaaacaatcc aaataagtaa acactgtatt tccctcaacg gttcccggat cttcaacttt
     2461 taaatgattt ggatctgtat acatactcat tacctttttc cgaacatctt cactagtatc
     2521 cgcaagataa atacaatttc caattgactt tgacattttt gttttgccat ctgttcccgg
     2581 caatctttga caaacttcat tttcagccaa ca
//
LOCUS       DAOMTA010000417         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1768_length_2612_cov_1.795072, whole genome shotgun sequence.
ACCESSION   DAOMTA010000417 DAOMTA010000000
VERSION     DAOMTA010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1768_length_2612_cov_1.795072"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLK68_10745"
     CDS             <1..222
                     /locus_tag="PLK68_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90920.1"
                     /translation="DIEDVLIGIICQKMLYHKYTHQPLILPEYLNRQKILTFFNNEPV
                     KYQTFEMNAKYLLKNNLVDYTQVAGIIYE"
     gene            215..1207
                     /locus_tag="PLK68_10750"
     CDS             215..1207
                     /locus_tag="PLK68_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM90921.1"
                     /translation="MNDDLQLLENIANFLKQGYLIKDVLNLCLIIHQTEKIKLIEQNL
                     VAGKAFDEVIIEIDFDNTFIEYFKFFRFKNNISTAIEHSLKICRKKEQIFTQLKKELT
                     YPVLLIMFFSLFIVYGLLPSIMQLFVEFNISPSIIIRIIFKLFEIIPIIVIFIILSFT
                     VFFTISIYAIKRKYFKLIDLLIEKIVLIRRLIQKYYSLKFALYYNELLINGYDSTDII
                     VMLYEQIDDSDIKMIIYEIYRQVLEGEALEDIINDFEYFEPLFIAYFKLLIHDNQKDK
                     SLDNYLRVSIDTLHMQVTRLIKLFVPIIYCFVAGFVILVYVAIVIPMMNVVSNL"
     gene            1220..1525
                     /gene="comGC"
                     /locus_tag="PLK68_10755"
     CDS             1220..1525
                     /gene="comGC"
                     /locus_tag="PLK68_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HRM90922.1"
                     /translation="MKRNNGFTLIEMIFCISVILVILLLVIPNVTSKNRVVKEKSCDA
                     QIEVVNSQIVLYEIENGELPTSISDLTSGEHPYLTEKQATCPSGLRISISDGQAYVR"
     gene            1515..>1660
                     /locus_tag="PLK68_10760"
     CDS             1515..>1660
                     /locus_tag="PLK68_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM90923.1"
                     /translation="MYDDSGFTLIEVVFTISIILILSTITLHFVINSKPVISLQQQCQ
                     QVIS"
     assembly_gap    1661..1670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1671..1932
                     /locus_tag="PLK68_10765"
     CDS             <1671..1932
                     /locus_tag="PLK68_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM90924.1"
                     /translation="LEEGKSRAMINHEQINIVIQTKQISYNGQKDQRTLTINDNYYID
                     DYYEFHFNHNGNISSGGHLKICSSNGCKSIILNVGSGAFYVK"
     gene            1922..2143
                     /locus_tag="PLK68_10770"
     CDS             1922..2143
                     /locus_tag="PLK68_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90925.1"
                     /translation="MLNKQGMTLIETLMAFSIFISIIVLFLSCYNNAINHHYQINQDY
                     MNYLKQQQEKEVELWQTSGLSESVNEVLH"
     gene            2095..2556
                     /locus_tag="PLK68_10775"
     CDS             2095..2556
                     /locus_tag="PLK68_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRM90926.1"
                     /translation="MADKWFERISQRGFTLIEVLFSLSICLLIILNSVPILRIVTAKD
                     KLSFNLSSYALGVKQISSILHTAKDIEINDDLTYTNANNEIFTISLNHHRIVKEPGFD
                     IIIHNVDELNFYQKNKNIYMKIIVDNKEHVYLIATDYQISENEKDEVETVL"
BASE COUNT          972 a          285 c          394 g          951 t
ORIGIN      
        1 gatatagagg atgttttaat tggcataata tgccaaaaaa tgctctatca caagtataca
       61 catcagccct taattttacc agaatattta aatcgtcaaa aaattttaac ttttttcaat
      121 aatgaacctg ttaagtatca aacatttgaa atgaatgcaa aatatttatt aaaaaataat
      181 ttagttgatt atacacaggt tgcaggaatt atttatgaat gatgatttac aattattaga
      241 aaatatcgct aactttttaa aacaaggtta tttaatcaaa gatgttttaa atttatgtct
      301 gataatccat caaactgaaa aaataaagct gattgaacaa aatttggttg ctggtaaagc
      361 atttgatgag gttattatag aaattgattt tgacaataca tttattgaat actttaaatt
      421 ttttcgattt aaaaataata tttcaactgc aattgagcat agcttaaaaa tatgcagaaa
      481 aaaggaacaa atatttacac agctaaaaaa agaattaacg tatccagttt tactcattat
      541 gtttttttca ttgtttattg tatatggatt attaccatca atcatgcaat tgtttgtcga
      601 atttaatatt agtccaagta ttataattag aattatattt aagctttttg aaattattcc
      661 aattatagta attttcatta tacttagttt tacagtattt ttcacaataa gtatttatgc
      721 tattaaaaga aaatatttta aacttattga tttgttaatt gaaaaaatag ttttaataag
      781 aaggttaatc caaaaatact attcactaaa atttgctctg tactataatg aattactgat
      841 caatggctat gattcaactg atattattgt catgctttat gagcaaatag atgatagtga
      901 tattaaaatg ataatttacg aaatttatcg acaagtttta gaaggagaag ctttagagga
      961 tataataaat gattttgaat attttgaacc attatttata gcatatttta aattattaat
     1021 tcatgataat caaaaagata aatcactcga taattattta agggtatcta ttgatacttt
     1081 gcatatgcag gtaacgagat tgattaaact ctttgtacca ataatatatt gttttgtggc
     1141 tggttttgta attttagttt atgttgcaat tgttattccg atgatgaatg tagtcagtaa
     1201 tttataggag gtatgaaaga tgaaaaggaa taatggcttt acattgattg agatgatttt
     1261 ttgtatttca gtaattttag ttattttgtt acttgttatt cctaatgtaa ctagtaagaa
     1321 tcgggttgtt aaagaaaaga gttgtgatgc gcaaatagaa gtagttaatt cacaaatagt
     1381 tttatacgaa attgaaaacg gagaacttcc aacaagtatt agtgatttga cttctggaga
     1441 acatccatat ttgacagaaa agcaggcaac atgtccaagc ggacttagga tttcaatcag
     1501 tgatggacaa gcatatgtac gatgatagcg gattcactct gattgaagtt gtctttacaa
     1561 ttagtataat cttaatttta agtacaatta cacttcattt tgtaattaat tcaaaaccag
     1621 taatttcttt acagcagcaa tgtcaacagg taatttcatt nnnnnnnnnn gttagaagaa
     1681 ggaaaaagta gagctatgat caaccacgag caaataaata tagttattca aacaaaacaa
     1741 attagttata atggacaaaa ggatcaacga actcttacga ttaacgacaa ctattatatt
     1801 gacgattatt atgagtttca ctttaatcat aatggaaata tcagttcggg tggtcatcta
     1861 aagatttgta gttcaaatgg atgtaaaagt ataattttaa atgttggaag tggagcattt
     1921 tatgttaaat aagcaaggga tgacattaat tgaaacattg atggcttttt ctatttttat
     1981 cagtattata gtattgtttc tttcttgtta taataatgca ataaatcatc attatcagat
     2041 taatcaggat tatatgaact atttaaaaca gcaacaagaa aaggaggtag aactgtggca
     2101 gacaagtggt ttgagcgaat cagtcaacga ggttttacat tgattgaagt tcttttttct
     2161 ttatcaatat gtttattaat tattttaaac agtgtcccca ttttaagaat agttactgca
     2221 aaagataaat tatcctttaa tctttctagt tatgctcttg gagttaaaca aatttcatca
     2281 attttgcata cggctaagga tattgaaatc aatgatgatt taacttacac aaatgcaaat
     2341 aacgaaatct ttactatttc actaaatcat cataggatag taaaagagcc aggttttgat
     2401 atcattatac acaatgttga tgaattaaat ttttatcaaa aaaataagaa tatttatatg
     2461 aaaataatag tcgataataa agaacatgtt tatcttattg ctactgatta tcaaataagt
     2521 gagaatgaaa aagatgaagt tgaaacagta ttgtaataat cggggtacga ttcttcaagt
     2581 cgtgttagtt atttttattg ttttaatctt aa
//
LOCUS       DAOMTA010000418         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1781_length_2591_cov_2.242114, whole genome shotgun sequence.
ACCESSION   DAOMTA010000418 DAOMTA010000000
VERSION     DAOMTA010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1781_length_2591_cov_2.242114"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..>2591)
                     /locus_tag="PLK68_10780"
     CDS             complement(242..>2591)
                     /locus_tag="PLK68_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HRM90927.1"
                     /translation="LGSIWDPYGNTKEIPVAVVNEDKKVEYNDSTLNVGKELAKSLQN
                     NDSMDFKLVDSKTADQGLKDGDYYMVITIPSNFSKNATTLLDAQPQKMILNYTTNPGS
                     SYIASKMDDSAIAKIKAEVSSTVTKTYAETIFNQLGTVGSGMSEAADGSNQINDGANQ
                     LISGNKTISDNLQVLATSSITFKDGASTLTNGLQDYTDGVVTVNNGVYSLKDGINSLN
                     NATPALSSGINQLDNGATELQTGISQYTGGVTSAYQGSQALVSNNSTLSNGVDTLAAG
                     ASQLKAGNQQISDSLTQMASQVKTSSISLSNYTTLINTLQNSGNADYKKLAQTILDTG
                     LSKEEVEQYGLTKFGVTDALPSSYQLVQQMSSSLSTMNTALNGDTTTPGLATASRTIQ
                     AGLNNLDASISGGEYTNTDGSKTTIPTEKSLKTGIKSYLAGTSQINSGLAQLNDNSSS
                     LVDGANKLKTGTSQLASQTPTLTNGIGALDQGAKQLADGTSTLVSNNPTLLSGADQLA
                     DGANQISDGAGQLAAGSTTLGTGLTTLQDGANTLATSLHDGAEQVNSINSNDSTFDML
                     ASPVDTSHKEISTVENNGHGMAPYMMSVGLYVACMAFTLMYPLFNDVEKAESGFKYWL
                     SKASVWFTVSTIASIVMIASLMFFCDFAPQQLLMTFIFAVIVGAASMALVTLLSIVCG
                     KIGEFVLLVFMVINLGGSAGTYPLETSSAIFKAIHPFMPFTYSVDGFRKVISMSNVSL
                     NTEIIVFVGIIIICSLLTILVYNHRIKKPTPLIPQAFENVNE"
BASE COUNT          829 a          460 c          420 g          882 t
ORIGIN      
        1 gtcttgcgat tattttagta attatttata tactgatttt aagaatgaaa aaaagatgag
       61 aaattaaatt tttatgcatt tacttgagtg gaacataaaa acagtacaag aaattgtact
      121 gtttttatgt tccaatatgt gtttataaaa tcaaaggtat taaaagtcat tttaatggga
      181 taaaaagtgt attttaaaag caaaagacag tatagaatcc tacactgtct tttgctttta
      241 tttattcatt aacattttca aatgcttgag gaattaatgg agttggtttt ttaattcgat
      301 gattataaac cagaatggtt aataaactgc aaataataat aattcctaca aatacaatta
      361 tttctgtatt caatgaaaca tttgacatac taataacttt tctgaagcca tcaacgctat
      421 atgtgaatgg cataaaagga tgaattgctt taaaaattgc acttgatgtt tctagtggat
      481 aagtaccggc cgaaccaccc aaattaataa ccatgaagac taagagtaca aattcaccaa
      541 ttttaccaca cacaatactt aacaaggtca ctaatgccat tgaagccgct cctacaatta
      601 cagcaaagat aaaagtcatt agtaactgtt gcggagcaaa atcacagaag aacatcaatg
      661 atgcaatcat tacaattgaa gcaattgtag atactgtaaa ccagactgaa gctttagata
      721 accaatattt aaagccgctt tctgcttttt caacatcatt gaataatgga tacatcaacg
      781 taaatgccat acaagctaca tataaaccta cactcatcat atatggagcc attccatgac
      841 cattgttttc aactgttgaa atttccttat gagaagtatc tacaggacta gcaagcatat
      901 caaatgtgct gtcgttactg ttaatactat ttacctgctc tgcaccatca tgaagtgaag
      961 tagctaaagt atttgcgccg tcttgtaagg tggttaaccc agttccaaga gtagttgatc
     1021 cagctgctaa ttgtccagcg ccgtcactga tttgattagc tccatcggct aactgatcag
     1081 caccgctgag caaagttgga ttattgctta ctaaagtact agtaccatca gctaattgtt
     1141 ttgctccttg atcaagggca ccgataccgt tagttagagt tggtgtttgg ctagctaatt
     1201 gagaagtacc agtttttaat ttattggcac catctactaa agaacttgaa ttatcattta
     1261 attgagctaa gccactattg atttgacttg ttccagcaag atagctttta attccagttt
     1321 ttaaactttt ctctgtggga atagtagttt tactaccatc agtgttagta tattcaccac
     1381 cactaattga agcatcaaga ttatttaaac cagcttgtat agttctacta gcagttgcta
     1441 gacctggggt agttgtatca ccatttaagg ctgtattcat cgtactaaga ctagaactca
     1501 tctgctgaac caattgatat gaagaaggca aagcatcagt tacaccaaac tttgttaatc
     1561 cgtattgttc aacttcttct ttacttagac ctgtatccaa aattgtttgt gctagctttt
     1621 tatagtctgc attaccacta ttttgaagtg tattgattaa ggtagtataa tttgataaag
     1681 aaatactaga tgtttttact tgtgaagcca tttgtgttaa gctatcactg atttgctggt
     1741 taccagcttt taattgactt gccccggctg cgagagtatc tacaccatta cttaaggtag
     1801 aattattgct aactaaagct tgagaacctt gataagcgct agtaacaccg ccagtatatt
     1861 gacttattcc agtttgaagt tctgtggctc cattatcaag ttgatttatt ccacttgata
     1921 atgcaggagt agcgttgttt agagaattga taccatcctt taatgaatat acaccattat
     1981 ttacagttac tacaccatcg gtataatctt gtaacccgtt tgtaagggta gaagccccat
     2041 ctttaaaagt gattgaacta gttgctaata cttgaagatt atccgaaata gttttattac
     2101 cactaattaa ctgattagcc ccatcattaa tttgattaga accatcagct gcctcactca
     2161 tgccgcttcc aactgttccc aattgattga aaatagtttc agcataagtc ttagttacgg
     2221 ttgaagaaac ttcagctttg attttagcaa ttgctgaatc atccatttta gaagcaatgt
     2281 aactgctgcc aggattagta gtatagttta aaatcatttt ttgtggttgg gcatctaata
     2341 atgtcgttgc atttttagaa aaattactag gaatagtaat taccatataa taatcaccgt
     2401 cttttaatcc ttgatcagca gttttactgt caaccagttt aaaatccatt gaatcattgt
     2461 tttgaagact ttttgctaac tctttaccga catttaaagt ggaatcatta tattcaacct
     2521 ttttatcctc attaactacc gctacgggaa tttctttagt atttccatac ggatcccata
     2581 ttgaccctaa g
//
LOCUS       DAOMTA010000419         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1784_length_2589_cov_1.852802, whole genome shotgun sequence.
ACCESSION   DAOMTA010000419 DAOMTA010000000
VERSION     DAOMTA010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1784_length_2589_cov_1.852802"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="PLK68_10785"
     CDS             <1..133
                     /locus_tag="PLK68_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRM90928.1"
                     /translation="QTVQDLAAKSEDDMIKVRNLGKKSLKEVKEKLVELGLGFKPID"
     gene            166..528
                     /gene="rplQ"
                     /locus_tag="PLK68_10790"
     CDS             166..528
                     /gene="rplQ"
                     /locus_tag="PLK68_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRM90929.1"
                     /translation="MAKNRKLGRTSDIRKSMLRSLATEVIMYGKLETTETRAKEVRSV
                     VEELITLGKRGDLHARRQAAAVLHNVVDAETGKTAVQKLFDDVAPKYSDVNGGYTRIL
                     KTYNSKGDNAPMAIIALV"
     gene            628..1458
                     /locus_tag="PLK68_10795"
     CDS             628..1458
                     /locus_tag="PLK68_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRM90930.1"
                     /translation="MDKIIEIKDLSFEYEAGLKTINHISFDINKGDYVAILGHNGSGK
                     STIAKLLIGLLEKKSGSIIINGYELNLENLYKVRDNIGIVFQNPDNQFIGATVRDDIA
                     FGLENTCVPQSDMDKIINTYAAKVGMSDFLDHEPTKLSGGQKQRVAIAGILAMAPTII
                     ILDEATSMLDPMGRREINSLVKELNKEKDITIISITHDIEEAKNADQVIMLSAGEVVA
                     SGNPKDILSDEANLVKYELDIPFALKVAKGLEKLNIRTSMSLQEEVLITELCQLHLKK
                     "
     gene            1434..2285
                     /locus_tag="PLK68_10800"
     CDS             1434..2285
                     /locus_tag="PLK68_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRM90931.1"
                     /translation="MSITFKEVEHIYSENTPFAYHALKGVNLKITDQSFTAIIGQTGS
                     GKSTLIQHINALLLPTSGSINIDEYLITATDKPSKLKPLRKKAGLVFQFPEYQLFEET
                     IEKDIIFGPMNFGVSEEEAKKIAHNVLKTVGLDESYLNKSPFDLSGGQKRRVAIAGIL
                     AMNPDILILDEPTAGLDPQGTNEMMSLFKRINESGKTIILVTHDMNHVLQYCDEVVVM
                     NHGKVEKHDTVTNVFKDSEYLNSLGIDLPIITNFIIKLNNQGFNLDSSINNIEQLIAA
                     IGGELNG"
     gene            2278..>2589
                     /locus_tag="PLK68_10805"
     CDS             2278..>2589
                     /locus_tag="PLK68_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HRM90932.1"
                     /translation="MDNMTFGKYIPVNSLIHQLDPRLKIGALLIFLIAIFFDAGFIGY
                     GIMGVFVLLVAGLSNISIKHILKAIKPMVFMMLFLMIFNIFLINTGSVLFTIGSLKIY
                     SG"
BASE COUNT          929 a          331 c          499 g          830 t
ORIGIN      
        1 ccaaactgtt caagatcttg ccgctaaatc tgaagatgat atgatcaaag taagaaactt
       61 aggtaagaaa tcgttaaaag aagtaaaaga aaaattagtt gaattagggt tagggtttaa
      121 acctatcgat taattcgcga gtacgaagga aggagcgcta atctcatggc taaaaataga
      181 aaattaggac gtacttctga tattagaaaa tcaatgttac gttctcttgc aacagaagta
      241 attatgtacg gtaaattaga aactacagaa actagagcta aagaagttcg ttctgttgtt
      301 gaagaattaa ttactttagg taaacgcggt gacttacacg ctagacgtca agctgctgct
      361 gtattacaca atgtagttga tgctgaaact ggaaaaactg cagtacaaaa attatttgat
      421 gatgttgcac ctaaatatag tgatgtaaat ggtggataca ctcgtatctt aaagacttac
      481 aacagtaaag gtgacaatgc cccaatggca atcatcgctt tagtttaatt gttaaagacc
      541 ctcgggtctt ttttctttta ctttgcttga ctaagtcaag taataataat agaatgttta
      601 ggagtagtta aacgggtggt gaaaaagatg gataaaatca ttgaaataaa agatttatca
      661 tttgaatatg aagctggtct aaagacgatc aatcatattt cttttgatat aaataaagga
      721 gattatgttg ctattttagg acataatggt agcggaaaaa gtacgattgc aaagttacta
      781 attggactac tagaaaaaaa gagcggttca attattatta atggatacga acttaatttg
      841 gaaaatttat ataaagtcag agataatata ggaattgttt ttcaaaatcc tgataatcag
      901 ttcattgggg caacagttcg tgatgatata gcctttggtt tagagaacac atgtgtacca
      961 caaagtgata tggataaaat cattaatact tatgcagcta aagtaggtat gagtgatttt
     1021 cttgatcatg agccaactaa attgtctggg gggcaaaagc agcgagtggc aattgctggt
     1081 attttagcaa tggctccaac aatcattatt ttggatgaag caacaagcat gttagatcca
     1141 atgggacggc gtgaaattaa tagtctagtt aaggaattaa ataaagaaaa agatattacg
     1201 attatttcta taactcacga tattgaagaa gctaaaaatg ctgatcaggt aattatgttg
     1261 agtgctggtg aagttgttgc aagtggtaat cctaaggata tcctaagtga tgaagccaat
     1321 ttggtcaaat atgaattaga tatcccattt gctttaaaag tagctaaggg tttagaaaaa
     1381 ttaaacatta ggactagcat gtctttacaa gaggaggtat taattacaga attatgtcaa
     1441 ttacatttaa agaagtagaa catatttata gtgaaaatac cccttttgct taccatgctt
     1501 taaaaggagt aaatttaaaa attactgatc aaagctttac agcgattatt gggcagaccg
     1561 gtagtggaaa gagtactttg attcaacata tcaatgcttt gttactacct acaagcggga
     1621 gtatcaatat tgatgaatat ctaattacag caaccgacaa accaagtaaa ttaaaaccac
     1681 ttcgtaaaaa agcagggcta gtttttcagt ttcctgaata tcaattattt gaagaaacaa
     1741 tcgaaaagga tattattttt ggacctatga attttggagt cagtgaagaa gaggctaaaa
     1801 aaattgctca taacgttctc aaaacggtag gattagacga aagctattta aataaatcgc
     1861 cttttgattt gtcaggtggt caaaaacgcc gagtagcaat tgctggaatt ttggcaatga
     1921 atccggatat tttaattttg gacgaaccaa ctgctggatt agatccgcaa gggactaatg
     1981 aaatgatgag tttatttaaa cgaataaatg aaagtggtaa aactattatt ttagtaactc
     2041 atgatatgaa tcatgtttta caatattgtg atgaagtagt agtaatgaat cacgggaaag
     2101 ttgaaaaaca tgatacagtc actaatgttt ttaaggatag tgagtattta aatagcttgg
     2161 ggatcgattt accaatcatt actaatttta tcataaaact aaataatcaa ggatttaatc
     2221 ttgattcttc aattaataat attgaacagc tcattgcagc gattggcggt gaattaaatg
     2281 gataatatga catttggtaa atatattcct gttaattctt taattcatca attagatccc
     2341 cgattaaaaa taggagctct actaatcttt ttaattgcta ttttctttga tgcagggttt
     2401 attggatacg gtatcatggg agtatttgtt ttgttagttg caggattatc aaatatttct
     2461 attaaacata ttttaaaagc aattaaaccg atggttttta tgatgctctt cttaatgatt
     2521 tttaatattt ttttaatcaa tacaggaagt gtattattca caattggatc acttaagatt
     2581 tattcgggg
//
LOCUS       DAOMTA010000420         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1793_length_2583_cov_1.835443, whole genome shotgun sequence.
ACCESSION   DAOMTA010000420 DAOMTA010000000
VERSION     DAOMTA010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_2583_cov_1.835443"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="PLK68_10810"
     CDS             complement(<1..1836)
                     /locus_tag="PLK68_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90933.1"
                     /translation="MALQLINITKFYKYGKKRQRVIDDLTIKFPSRGMIGIIGQSGTG
                     KSTLLNIIAGIERPCNGQVLIDGHRLNYQQIRMYQRDYISYVYQFYNLVNALTIKENL
                     ILITKIKGIDLKQVSGQLEEYCRTLKVVELLERYPDELSGGQKQRIGLVRAFLCNTPI
                     LLADEPTGALNDKLSIEVMRLLKRYAKEHLVIVISHNSRLIKQYTKMIVDLDKKKNYY
                     NFSNERYHKYSPIIIKKQPGRLLFYCKRQLKYQFKKIIMMFVSQIFIILAFVLLLSAF
                     NGGWQYLEKKFEEDPLKRVIEVSKNDYINNIFERKEIKKFEKNNKIANVAYKLVFSNG
                     ILSSYDEELDVEVYQIFKTKKLDYISGKYPSKENQILINQTTAKKYKLKIGERINFEI
                     NDDEYEFTISGIINDHINIGTNLYLEQQYLEEELNDDCLDKTVLIFHSDQVDAVIKEY
                     QKDYLLINLHGDYIDNYRSIFDITRFVIFLFIVISFAISLILISVILKTIFIERKRDT
                     SLLLANGLGKGKAINLFCREAILIGGLIGSAGAILAMGVLRLIELFDLSDYLFNIPRL
                     FVLPKYIFTKYDLYVVLIVIYIIACYLAGLQASLKINRMDISILLK"
     gene            1874..>2583
                     /locus_tag="PLK68_10815"
     CDS             1874..>2583
                     /locus_tag="PLK68_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538061.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRM90934.1"
                     /translation="MNLFKYSDDNKRYHTFNYYLKNKYQSKVAKVSLNAGFTCPNRDG
                     TKGTGGCIFCSSTGSGDFAGNIQDSLEIQFSNVSLLLQNKWPNCKYIAYFQANTNTYG
                     PLKKIKACIEPFINKKNVVAISIATRPDCLETDVLNYLAEVNNRCDLWVELGLQTIHD
                     QTGELINRGHNYQDFLDGLNKLRKLKINVCVHIINGLPGETYEMMLETAKAVGQLDIQ
                     ALKIHMLYVIEKTKLHQL"
BASE COUNT          908 a          439 c          311 g          925 t
ORIGIN      
        1 ctttaacaat atgctaatat ccattctgtt tattttaaga ctagcttgta accctgctaa
       61 ataacatgca attatataaa taacaatcaa cactacatat aaatcatatt ttgtaaaaat
      121 atatttcggt aagacaaaca accttggaat gttaaataaa taatcactta aatcaaatag
      181 ctcaattaat cttaatacgc ccattgctaa gattgctccc gcacttccaa tcagccctcc
      241 tatcaaaatt gcttcacgac aaaataaatt aattgcttta cctttaccaa gaccattagc
      301 caataaaaga ctagtatctc tttttcgttc aataaaaatt gttttaagaa taactgaaat
      361 taaaattaat gaaatagcaa aactaattac aataaataag aaaatcacaa atctagtgat
      421 atcaaaaatt gatcgatagt tgtcaatata atcaccgtgt agattaatca gcaaataatc
      481 tttttgatat tcttttatca cagcatctac ttgatcacta tgaaatatta aaactgtttt
      541 atccaggcaa tcatcattta actcttcttc taaatactgt tgttctaaat aaagattagt
      601 ccctatatta atatgatcat taatgattcc actaatagta aattcatatt catcatcatt
      661 aatttcaaaa ttaattctct cacctatttt taatttatac ttctttgccg ttgtctgatt
      721 aattaatatt tgattctctt ttgaaggata tttcccagaa atataatcta actttttagt
      781 tttaaatatc tgatatacct caacgtctaa ctcttcatca taactgctta aaattccatt
      841 actaaatact aatttgtaag caacatttgc tattttatta ttcttctcaa attttttaat
      901 ttctttgcgt tcaaagatat tattaatata atcatttttt gaaacttcaa taactctttt
      961 taatgggtct tcttcaaatt ttttttctaa atattgccat ccaccattaa aagcacttaa
     1021 taataaaaca aaagctagaa taataaatat ttgtgataca aacatcatta ttatcttttt
     1081 aaattgatat ttgagttgcc ttttacaata aaaaagcagt cgacctggct gcttttttat
     1141 gattattggt gaatatttat gatatcgttc attactaaaa ttataataat tctttttttt
     1201 atcaagatct acaatcattt ttgtatattg tttaattaat cgagaattat gcgaaataac
     1261 aatcactaaa tgctccttgg catacctttt taaaagtctc atcacttcaa tacttaattt
     1321 atcatttaat gctccagttg gttcatctgc taaaagaatt ggtgtattac acaagaaagc
     1381 tcgaaccaat ccaattcgct gcttttgacc acccgatagt tcatcgggat atctttccaa
     1441 caattcaact acttttaaag ttcgacaata ctcctccaat tgaccagaca cttgctttaa
     1501 atcaatacct tttatttttg taattaagat taaattctct tttattgtta aagcattgac
     1561 taaattataa aattggtaaa cataactaat ataatcacgt tgatacattc taatttgttg
     1621 atagttgagc ctatgcccat caattaatac ctgaccattg catggacgct caattcccgc
     1681 aataatgttt aacaaagtcg atttccctgt accactttga ccaatgattc ctatcatccc
     1741 tctacttgga aatttaattg ttagatcatc aataacgcgt tgtctctttt ttccatactt
     1801 ataaaattta gtaatattaa taagttgtaa tgccataacg tcctccttat gttataatag
     1861 caatggtgat aaaatgaact tatttaaata tagtgatgat aataaacgct atcatacttt
     1921 taattattat ctgaaaaata aatatcaaag taaagttgcc aaagttagtt tgaatgcggg
     1981 ctttacttgt ccaaatcgcg acggtactaa aggcacgggc ggctgtattt tttgtagcag
     2041 cactggttca ggtgattttg ccggaaatat ccaggattca ttagaaatac aatttagtaa
     2101 tgttagtttg cttcttcaaa ataaatggcc aaattgtaaa tatattgctt attttcaagc
     2161 caatactaat acatacggcc cattaaaaaa aatcaaagct tgtatcgagc catttatcaa
     2221 taaaaaaaat gtggttgcaa tcagcatcgc aactcggcca gactgcttgg aaaccgatgt
     2281 tttaaattat ttagcagaag ttaataatcg ctgcgattta tgggtcgaat taggtttaca
     2341 gaccattcac gatcaaacag gtgaactaat caatcgtggc cataattatc aagacttcct
     2401 agacggcttg aataaattac gaaaattaaa aattaatgtc tgtgtacata ttatcaatgg
     2461 tctacctggt gaaacatatg aaatgatgct tgaaacagca aaggcggttg ggcagttgga
     2521 cattcaagcc ttaaagatcc atatgcttta tgtcattgaa aaaacaaaat tacatcagtt
     2581 ata
//
LOCUS       DAOMTA010000421         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1794_length_2583_cov_1.818434, whole genome shotgun sequence.
ACCESSION   DAOMTA010000421 DAOMTA010000000
VERSION     DAOMTA010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1794_length_2583_cov_1.818434"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..952)
                     /gene="era"
                     /locus_tag="PLK68_10820"
     CDS             complement(62..952)
                     /gene="era"
                     /locus_tag="PLK68_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRM90935.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLLNSILETKLAIMSDVAQTTRNTIQ
                     GIHTDDEAQIIFMDTPGIHKPQDRLGTFMNTTALNSIFGVDLVLFLAPANEKIGRGDK
                     FIIERLKEADGPVFLVLSKIDTVSKEELIKKLQEWQELFDFKEIIPISATTNDNIDLL
                     LKTVKAYLPEGNMYYPQDHLTDHPERFVMAEFIREKILYFTKEEVPHSVAIVIERMLE
                     DEAGVEIIATIVCDRKSQKGIIVGKQGTMIKKIRQNAQREMKRFLQVPVHLELFVKVE
                     NNWRNKQKYLKEFGYNEDDY"
     gene            complement(931..1347)
                     /gene="cdd"
                     /locus_tag="PLK68_10825"
     CDS             complement(931..1347)
                     /gene="cdd"
                     /locus_tag="PLK68_10825"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRM90936.1"
                     /translation="MDVKTIIEKAFEAAKNSYSPYSKFKVGACIEMRDGNYFLGTNIE
                     NAAFGSTMCAERNAVYGAYSNGYGKADIKQLAIVAGSDEIVSPCGACRQVLMELLPQD
                     CPVILANRESYEITTMKELLPRAFIGESLTCSNQDL"
     gene            complement(1425..1790)
                     /gene="ybeY"
                     /locus_tag="PLK68_10830"
     CDS             complement(1425..1790)
                     /gene="ybeY"
                     /locus_tag="PLK68_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRM90937.1"
                     /translation="MGIEEDLELSCILVDDSKIHEINKEYRKIDRATDVISFALEDNE
                     QFYVPGMPRSIGDIFISVDHAKVQAEEYGHDLKREMCFLFTHGLLHLLGFDHMIEEDE
                     IQMIAMQKSILDALNITRR"
     gene            complement(1895..>2583)
                     /locus_tag="PLK68_10835"
     CDS             complement(1895..>2583)
                     /locus_tag="PLK68_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM90938.1"
                     /translation="YKEIIPNRSEKKQKIRGRISKISFDHISYSYGYSKPILQHLDLQ
                     IERSLWLKGDTGVGKSTLLKLLMKYDELLKGHIYINGIDLMNIDTNSLYRKIIYLDRK
                     PVFYHESLRFNLILNSKEEELMEKLLKIFGLNYYLDRLDLIIETDGQPLSSGQAQIVM
                     LIRAIIKKPEVLILDEALCNIDDYKAKKIISYIHDNLPNTIMIIVAHQTKLVNELFDC
                     AIIRDGKIYK"
BASE COUNT          822 a          522 c          298 g          941 t
ORIGIN      
        1 catatcattt tctttataag gcttattttt taaaactagt ccagtaatat tttcttcacc
       61 attaataatc atcttcatta tatccaaatt cttttaaata tttttgtttg ttgcgccaat
      121 tattttcaac cttaacaaac aactctaaat gaactggtac ttgtaaaaaa cgtttcatct
      181 cgcgttgagc attctggcga atctttttga tcatcgtccc ttgtttacct acaataattc
      241 ccttctgact tttacgatca caaacaattg ttgcaataat ttctacaccc gcttcatctt
      301 ccagcattct ttctatcaca attgcaacac tatgtggtac ttcttcttta gtaaaataaa
      361 gaattttttc acgaataaat tcagccatta caaaacgctc tgggtgatct gttaaatgat
      421 cttgaggata atacatattc ccttctggca aatatgcttt taccgtttta agtaataaat
      481 caatattatc attagtagtt gcactaattg gaataatttc tttaaaatca aataattcct
      541 gccactcctg taatttttta atcagctctt cttttgaaac agtatcaatt ttacttaata
      601 ctaaaaacac cggcccatcg gcttccttta agcgctcaat aataaattta tctccacgtc
      661 caattttttc attagccggt gctaaaaata aaactaggtc aacgccaaaa atgctattta
      721 aagcggttgt attcataaat gttcccaaac gatcttgcgg tttatgaata cctggtgtat
      781 ccataaaaat tatttgggct tcatcatcgg tatgaattcc ttgaattgta tttctagttg
      841 tttgagcaac atcagacatg atagctaatt ttgtctctaa aatactattt aatagtgtcg
      901 attttccaac attaggacga ccaacgatac ttacaaatcc tgatttgaac atgttaaact
      961 ttctcctata aatgcccgcg gcaataattc tttcattgtt gttatttcat aactttcacg
     1021 attagccaaa atcactggac aatcttgtgg taatagttcc atcaatactt ggcgacatgc
     1081 tccacatggt gaaacaatct catcactacc tgccacaatt gccagctgtt tgatatcagc
     1141 tttaccatag ccattgctat acgcaccata aacagcatta cgttctgcgc acatagttga
     1201 accaaacgct gcattttcaa tattagtccc taaaaagtaa tttccatctc tcatctcaat
     1261 acatgcccct actttgaatt ttgagtatgg actataagaa tttttcgctg cttcaaaagc
     1321 cttttcaatt attgttttta catccataaa accacttcta ctcaacttaa atcatgatat
     1381 tgagtattta tcctttctaa gtctataaat tagcaataaa ctacttatcg tcttgtgata
     1441 tttaaagcat ctaaaattga tttttgcatt gcaatcatct gtatttcgtc ttcttctatc
     1501 atgtgatcaa accctaaaag atgtaataat ccatgggtaa ataaaaaaca catttcacgc
     1561 tttaaatcat gaccatattc ttcagcttgg actttagcat gatcaacaga aataaaaata
     1621 tctccgatac ttcttggcat ccctgggaca taaaactgct cattatcttc taatgcaaaa
     1681 ctaattacat ctgtggcccg atctatttta cgatattctt tatttatttc gtgaatttta
     1741 gaatcatcta ccaagatgca tgataattct aaatcttctt caattcccaa ttgcagcttc
     1801 attgattatt gaacgataat catcagtata atcttcatta aaaccagctg cttcatttat
     1861 caatgcaaaa tctatttcca tacaattacc tcatttactt atatattttt ccatctctaa
     1921 ttatagcaca gtcaaataat tcattcacta attttgtttg atgcgctacg attatcataa
     1981 tcgtatttgg caaattatca tgtatgtagc ttataatttt cttagcttta taatcatcta
     2041 tattgcacaa tgcttcatcc aaaataagaa cttctggttt ttttatgata gctctgataa
     2101 gcattactat ctgggcttga cctgacgata aaggctgacc atctgtttca ataatcaaat
     2161 caagacggtc aagatagtaa tttaacccaa atatctttaa caatttttcc atcagttcct
     2221 cctctttact attcaaaatc aagttaaatc gcaatgattc atgataaaaa acaggttttc
     2281 gatctaaata aataatcttt cgatacaaag aattagtatc aatattcatt aaatcgatcc
     2341 cattaatata aatatgcccc tttaataact catcatactt catcagtaat ttaagtaagg
     2401 ttgattttcc caccccagta tcacctttta accataatga acgttcaatc tgaagatcca
     2461 aatgttgaag aatcggctta ctataaccgt aactatagct aatatgatca aaactaatct
     2521 tactgattcg cccccttatt ttttgctttt tttcacttct attaggaata atctctttat
     2581 aac
//
LOCUS       DAOMTA010000422         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1798_length_2580_cov_2.186139, whole genome shotgun sequence.
ACCESSION   DAOMTA010000422 DAOMTA010000000
VERSION     DAOMTA010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1798_length_2580_cov_2.186139"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1379
                     /locus_tag="PLK68_10840"
     CDS             9..1379
                     /locus_tag="PLK68_10840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM90939.1"
                     /translation="MSTFTKFKMQEFCLQTIEELGFQKPTPIQEQVIPLVYKNSDIIG
                     ISQTGTGKTHAFLLPVMDRIDPKNNSVQAVITAPTRELATQIYNNAKLFTKYNSEIKV
                     SLIVGGNDRQKTVNKLAVQPHVVIGTPGRIKDLSLDEQALKITTASTFIVDEADMTLE
                     FGYLEDIDAVAGKMRDDLRMMVFSATIPQMLRPFLKKYMKSPVLVEIDDDKVTTENVE
                     HILIATKHKNRYEVLKKIIESIDPYVCIIFANTRSDVASTTKKLREDGFKVGEIHGDL
                     EPRERKQMMRRIQNNEYQFIVATDIAARGIDIDGVSHVINMEFPKEPDFYIHRSGRCG
                     RGNYTGICYSMYDTTDEANLKVLERKGILFKNMTIKNGQFVDLGDRLKRNKRPKQQTE
                     LEKKVQSIIRKPKKVKPGYKKKRKREVEQVVRKQKRAMIQNDIRRQKKERARAAQAAK
                     RNGEEN"
     gene            1379..2239
                     /locus_tag="PLK68_10845"
     CDS             1379..2239
                     /locus_tag="PLK68_10845"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRM90940.1"
                     /translation="MIIGSHVSMSGKEMLLGSVKEALSYGANTFMFYTGAPQNTARKP
                     IDQLRIEEAKELMKENGIEIDNVVVHAPYIINLANTTKPETFELAVSFLKQEIERCKA
                     IGATRLVLHPGAHVGAGSQIGLERIVEGLNEATKDCGNVKIALETMAGKGSECGRTFD
                     ELQYIIENVDNNECLGVCMDTCHLHDAGFDLTKFDDILTEFDQKIGLERLLVVHVNDS
                     KNECGASKDRHENIGYGHIGFDTLNMIVHHDKLKSVPKILETPYIEKIAPYKEEIEMF
                     RTQTFNPRLK"
     gene            complement(2212..2481)
                     /locus_tag="PLK68_10850"
     CDS             complement(2212..2481)
                     /locus_tag="PLK68_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90941.1"
                     /translation="MSNTSYNELFNLDFKMFKKLVYEQDIDIDEEQLLLIYNLIQNNR
                     YALIDSHYNEVLYNYISEKTSITTCLKIKSFLSNCPHYFNLGLKV"
BASE COUNT          945 a          335 c          492 g          808 t
ORIGIN      
        1 agtacaaaat gagtacattt acaaaattta aaatgcagga attttgttta caaacaattg
       61 aagaattagg ctttcaaaaa ccaactccaa ttcaagaaca agtaattcct ttagtgtata
      121 aaaatagtga tattattggt atttcacaaa caggtacagg aaaaactcat gcctttttgt
      181 taccggtaat ggatcgcatc gatcctaaga ataatagtgt tcaagctgta attacagccc
      241 cgacacggga attagcaacc caaatctata acaatgctaa attatttact aaatataata
      301 gtgagatcaa agtatctttg atcgttggag gaaatgatcg tcaaaagacc gttaataaac
      361 tagcagttca accacatgtt gttattggga caccgggaag aattaaagat ttatctttag
      421 atgaacaagc tttaaaaatt acaactgctt caacttttat tgttgatgaa gctgatatga
      481 cgcttgaatt tggatatttg gaagatattg atgctgtagc cgggaagatg cgtgacgatt
      541 taagaatgat ggtcttttct gcaacgattc cgcagatgtt aagaccgttt ttaaaaaaat
      601 atatgaaatc accagtatta gtagagattg atgatgataa agtaactact gaaaatgtgg
      661 aacatattct aattgcaact aagcataaaa accgttatga ggtattaaaa aagattattg
      721 agtcgattga tccttatgta tgtattattt ttgctaatac tcgtagtgat gttgccagta
      781 cgactaaaaa attacgagaa gacggattta aagtgggaga gattcatggt gatctagaac
      841 ctcgtgaacg taagcaaatg atgcggcgaa ttcaaaacaa tgaatatcaa tttattgttg
      901 caacggatat tgcggctcgt ggaattgata ttgatggagt atcgcatgtt attaatatgg
      961 aatttcctaa agaaccggat ttttatattc atcgtagtgg tcgttgcggc cgtggaaatt
     1021 atactggaat atgttacagc atgtatgata caaccgatga agcaaattta aaagttcttg
     1081 aacgtaaagg aattcttttt aaaaatatga cgattaaaaa tggtcaattt gttgatttgg
     1141 gtgatcgttt aaaaagaaat aaacgtccta aacaacaaac cgaattagaa aagaaagttc
     1201 aaagtattat tcgtaaacca aaaaaggtaa aacctggtta taaaaagaaa cgtaaaagag
     1261 aagttgagca agttgttcgt aaacaaaaac gagcaatgat tcaaaatgat attcgccgac
     1321 aaaagaaaga aagagctcgt gcagctcaag cggctaagag gaatggagaa gagaattaat
     1381 gattattggt agtcatgttt caatgtcagg aaaagagatg ttattaggtt cagtcaaaga
     1441 ggcattatca tacggggcta atactttcat gttttacact ggagctcccc agaatactgc
     1501 ccgtaagcct attgatcaat tacgcattga agaagcaaaa gaattaatga aagaaaatgg
     1561 aattgaaatt gataatgttg ttgtgcatgc accgtatatt atcaatctag ctaatacaac
     1621 aaaacctgag acttttgaat tagcagtcag ctttttaaag caagaaatag aacgttgtaa
     1681 agcgatagga gcaacacgat tagttttaca tccaggtgcc catgtaggag ctgggagtca
     1741 gattggtttg gaaagaatcg ttgaaggatt gaatgaagca acaaaagatt gtggtaatgt
     1801 taaaattgct ttagagacaa tggctggtaa aggaagtgaa tgtggtcgaa ctttcgatga
     1861 attgcaatat attattgaaa atgttgataa taatgaatgt ttaggggtct gcatggatac
     1921 ttgtcatctt catgatgctg gatttgattt aactaagttt gatgatattt taacagaatt
     1981 tgatcaaaaa attggtttgg aacggttatt ggttgttcat gtaaatgatt ctaaaaatga
     2041 gtgtggagca agtaaagatc gtcatgaaaa tattggatat ggtcatattg gatttgatac
     2101 attgaatatg atcgttcatc atgataaatt aaaaagtgtt cctaagattt tagaaacacc
     2161 atatattgaa aaaattgctc catataagga agaaattgag atgtttagaa ctcaaacttt
     2221 taatcctaga ttaaagtaat ggggacaatt ggataaaaaa cttttaattt taaggcaagt
     2281 agtgatactt gtcttttcac ttatataatt gtagagaact tcattataat ggctatcgat
     2341 cagtgcatag cgattatttt gaattaaatt ataaattagt aataattgct cttcgtcaat
     2401 atcaatatcc tgctcatata caagcttctt aaacatctta aaatctaaat taaataattc
     2461 attgtaacta gtgttactca tcatgtttcc tccttttttt caattatatg cataaagtaa
     2521 aaaagtagta ctaaaatgta ctacttaatc attaataaaa tttctttttt taattcttca
//
LOCUS       DAOMTA010000423         2578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1801_length_2578_cov_3.293698, whole genome shotgun sequence.
ACCESSION   DAOMTA010000423 DAOMTA010000000
VERSION     DAOMTA010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1801_length_2578_cov_3.293698"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            898..2301
                     /locus_tag="PLK68_10855"
     CDS             898..2301
                     /locus_tag="PLK68_10855"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HRM90942.1"
                     /translation="MKPDTNTMSTDHNSDVLKIFGIDSNIIEHYDIFHRKDGVHIILK
                     LINREQRCPVCQTRTTKVVNYIDKKIKHSVVSTSSCFIDYHARRYRCPACNKTFYESN
                     PFTFGTERISSATVYSVLQDLKKPFETFKSVGERYHISTNTVINIFDRYISTPRLPMP
                     SVLSIDEVYVPTDDRNKYLCILFDFETKMIIDVLPSRHKNVLSAYFHSVPLDERKNVE
                     YMSSDMWVSYHDISKIFLPDTIRTIDRFHLLMEFSKKFNSIRCETMKKYALTKNAYQK
                     EKTKRKLKPDEEAIYREACINYYAFKKFSWLFYKTDSDILNPNNDKKFNRVFNRYMNF
                     YDIHEHICSCDNGFEEICNLKYDLDMFFKNSTVETAKENLENLIKDFKENDRDEMKNF
                     AGTLSRWKNEIIYSFTIIGESTINNGRIENINRTIKTIKRNANGYKNFERLRRRIMWC
                     VNKQGNIILSQRKENES"
     gene            2298..2486
                     /locus_tag="PLK68_10860"
     CDS             2298..2486
                     /locus_tag="PLK68_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90943.1"
                     /translation="MKKYTVIVDEDYQAFNENCNITLDEIDLKIQALNKELEDIKNIA
                     LKNYDILDKLFELRHVDF"
BASE COUNT          933 a          428 c          379 g          838 t
ORIGIN      
        1 caattataaa aaaattagat ataaaaaata ttgaaaatcg gctattacat atgggaatta
       61 ttatttattt aaaaaaataa attttaaaaa gcgcttcata taattgaagg gctttgttgt
      121 ttcggtttta tttcaagcca tgatatttga aactgctttt caaaatcttg tggttttaat
      181 atatgtagta aaaacagacc tcttctgttt catgaggtct gcttttttgc tttatattac
      241 tttgatatca tgtcataatt atttaaatat atagaaagta aaaaggatga tacccaacat
      301 cgccaaacgt cacttatcat cctctgtcaa aagacatgat tataataact gattactcta
      361 taaaactcaa tacttccaaa gctaaaaatg attttgatgc tcttataaaa ttcagaaaac
      421 tatattctga tctcatttct tcattttctt tatcttctat tgaatgcacc tgtggcagtc
      481 ataactggca tatgcattct tcctattctc gcttctatga ttttcttgga agaaaaatca
      541 aagtttccat tcagcgtatt atctgtgctt catgcggtaa aactcatgct ctgcttattg
      601 aagatatgat tcctttttct acttttacat tctctcaaat catcgactgt cttgatgatc
      661 ctgatttccc tgtggataac tttcacagat cttttctgat caacaaatat tataacgttg
      721 aacaaaatta ccgttcatac tgtcaaataa accgaagaaa tcatatgctt atttttcttc
      781 caacataact tttatctttt tttctgttct tttcattatc atttaagtaa agataaacag
      841 aagaagatga ttttgaaatg aatttgaaca aatttttata tagcgctggt gatttctatg
      901 aaacctgata ccaatactat gtccactgat cacaacagtg atgtattaaa aatatttggt
      961 attgatagta acatcattga acattacgat atcttccata gaaaagatgg tgtccatatt
     1021 atcttgaaac ttataaacag agaacagcgt tgccctgttt gccagaccag aacaactaaa
     1081 gttgtcaatt acatagataa gaaaataaaa cattctgttg tttccacttc ctcttgtttc
     1141 attgattatc atgcccgacg ttacagatgt cctgcatgca acaagacttt ttatgaatcc
     1201 aatcctttta cttttggaac ggaaaggatc tcgtcagcta ccgtttatag tgttctgcag
     1261 gatcttaaaa agccatttga aacatttaaa agtgttggtg aaagatatca tatatccacc
     1321 aatacagtca tcaacatatt tgacagatat atcagtacac caaggcttcc catgccatct
     1381 gtactttcaa tagatgaagt atatgttcca accgatgaca gaaacaaata tctctgcata
     1441 ctttttgatt ttgaaacaaa gatgatcatc gatgtcctgc catcaagaca taaaaatgtg
     1501 ttatcggcct attttcatag cgttcctctt gatgaaagaa aaaatgttga atatatgtca
     1561 tccgatatgt gggtatccta ccacgacata tcaaaaatat tcttacccga tactatcagg
     1621 acaattgaca gattccacct ccttatggag ttttcaaaaa aatttaattc aatcagatgt
     1681 gaaacaatga aaaaatatgc acttactaaa aatgcctatc agaaagaaaa aacaaaaaga
     1741 aaactgaaac ctgatgaaga agcaatctat agagaagcat gtatcaacta ttacgccttt
     1801 aaaaaattca gctggctttt ctataaaact gattctgata ttttaaatcc aaacaacgat
     1861 aagaagttca acagggtatt caaccgatat atgaattttt atgatatcca tgaacatatc
     1921 tgttcatgtg ataatggttt tgaagaaata tgcaatctaa aatatgatct cgatatgttc
     1981 tttaaaaaca gtacagtcga aaccgcaaaa gagaaccttg aaaatttaat aaaggatttt
     2041 aaagaaaatg accgtgatga aatgaagaac ttcgcaggta ctctttcccg ttggaaaaat
     2101 gaaatcattt attcttttac gatcatcggt gaatcaacta tcaataacgg gcgcatagaa
     2161 aatataaacc gaacgattaa gaccatcaaa agaaatgcga acggatataa aaactttgag
     2221 cgcttaagaa gacgcataat gtggtgtgta aacaagcagg gaaatataat actcagtcaa
     2281 agaaaggaaa atgaatcatg aaaaaatata cagtgattgt agatgaagat tatcaggcgt
     2341 ttaatgaaaa ctgcaatata acgctagatg aaattgattt aaaaatacag gccctaaaca
     2401 aggagttgga agacataaag aatatagctt tgaaaaacta tgatatccta gataaacttt
     2461 tcgaactcag acatgtagat ttttagacaa tataaaacca tgaggtgtct catttgagat
     2521 ttgcctcatg gtttctcatt agctctattt caaaaatatc atgttatttc ataaagcc
//
LOCUS       DAOMTA010000424         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1807_length_2573_cov_2.218427, whole genome shotgun sequence.
ACCESSION   DAOMTA010000424 DAOMTA010000000
VERSION     DAOMTA010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_2573_cov_2.218427"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..781)
                     /locus_tag="PLK68_10865"
     CDS             complement(101..781)
                     /locus_tag="PLK68_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRM90944.1"
                     /translation="MKKLGSKKTLPELAVEQIIGYINENGLQPGDQLPNEVDLMNKLK
                     VGRGTIREAIKSLNSKGVVIIKRGIGTFVADQPGISDDPLGFTFETDKTKVLMDALEV
                     RLLIEPQMIIKTIDNISAKQLKELSDLADEIESLINKNKDYTVQDIEFHTLLARVSGN
                     RVIGKLIPIIAGAIEQAIDVTDKSLVQETIITHRLIVEAVANKDKEGASKAMARHIQD
                     NIDILKTI"
     gene            914..>2573
                     /gene="ilvD"
                     /locus_tag="PLK68_10870"
     CDS             914..>2573
                     /gene="ilvD"
                     /locus_tag="PLK68_10870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM90945.1"
                     /translation="MKSQELRKIAPEIDPLRIGTGWSVADLSKPQIMIESTYGDSHPG
                     SVGLLDLVYEVEKGINDHGAKASKFFTTDICDGEAQGHDGINYSLASRDMIAGMIEIH
                     NNATPFDGSVFVASCDKGMPAHLMGAGRINNPCIFVTGGVMEAGPDMLTLEQIGKYSA
                     MYLRGEISKEQFEYYQHHACPSCGACSFMGTASTMQIMTEALGLMLPGTALMPATCKD
                     LKEQAYKAGKQIIKLAKMDLTARDIVTMKSFENAIMVHAAISGSTNSLLHLPAIASEF
                     GIDLNADLFDQMHQNAHYLLDIRPAGKYPAEYFYYAGGVPAVMEAIKENLNLDVMTVT
                     GKTLGENLEELKNNGYYENCQTFLDKINLKSTDIIRPYDDPIGKDGTIAILKGNLAPD
                     GAVIKHSACPQEMMQAVLRARPFDSEEAAIDAIIKKQIKPGDAVFIRYEGPKGSGMPE
                     MFYTGEAISSDQELAKSIALITDGRFSGASKGPVIGHVSPEASEGGPIALIEENDLIK
                     IDVPQRRLEIIGIEGKQRTSQEITKILKERHAKWKPKEPKYKNGI"
BASE COUNT          836 a          455 c          462 g          820 t
ORIGIN      
        1 atttccttat aaataaaaat gcttagttta ttcaatcaat ctccgatcgt tagttatcga
       61 atctaacttt aggcgatatc tcatctttac taagcttttt ttaaattgtc tttaatatat
      121 caatattatc ttggatatgt ctagccatcg ctttactagc tccttcttta tctttattag
      181 caacagcttc aacaatcaaa cgatgtgtaa taattgtttc ttggacaagg gatttatcag
      241 ttacatcgat agcttgttca atcgctccag caataattgg aattaatttt ccaataaccc
      301 gattaccgct aacccgagct aataaagtat gaaattcaat atcttgtaca gtataatctt
      361 tatttttgtt aattaagctt tcaatttcat cagctaagtc tgataattcc ttaagttgtt
      421 ttgcactaat attatcaatt gttttaataa tcatttgcgg ttctatcaac aatcggacct
      481 ccaaagcatc cattaaaact ttagttttat cagtttcaaa agtaaacccc agtggatcat
      541 cactgattcc tggctgatca gccacaaaag ttccaatccc acgtttaatt attacgactc
      601 cttttgaatt tagactttta atcgcttcac ggatcgttcc acgtccaact ttaagtttat
      661 tcattaagtc aacttcatta ggtaattgat cacctggttg aagaccattt tcgtttatat
      721 agccaataat ttgctctact gccaactctg gcagcgtttt tttacttcct aattttttca
      781 ttttctcgcc tcgcctataa attcttacac attttattat ttttgtcaat tttaattaac
      841 gctattgaca aacatcatca agccttttag aattaaatta tatttaagac gtcatacgtc
      901 ataggaggat aaaatgaaat cacaagaatt acgtaaaatt gcacctgaaa ttgatccact
      961 aaggattggt actggatgga gtgttgccga tctaagcaaa cctcaaatta tgattgaaag
     1021 tacttacggt gactcccacc ccggaagtgt tggtctttta gatctagtct atgaggttga
     1081 aaaagggatc aatgatcatg gtgctaaagc ctcaaaattt ttcactaccg atatttgtga
     1141 tggtgaggca caagggcatg acggtatcaa ttattctcta gcatctagag atatgattgc
     1201 tggtatgatt gaaattcaca ataatgcaac tccctttgat ggcagtgttt ttgttgcaag
     1261 ttgtgataaa ggtatgcccg ctcacttaat gggagccgga agaattaaca atccttgtat
     1321 ctttgtaact ggaggtgtaa tggaagctgg tccagatatg ctaaccttag aacaaatcgg
     1381 aaaatatagt gccatgtatt tacgtggcga aatctccaaa gagcaatttg agtattatca
     1441 gcatcatgcc tgtccaagct gtggagcttg tagtttcatg gggactgctt caacaatgca
     1501 gattatgacc gaagcattag gattgatgtt acctggtaca gcattaatgc cagctacttg
     1561 taaagattta aaagagcagg cctataaagc aggaaaacaa ataattaaat tagccaaaat
     1621 ggatttaaca gctcgcgata ttgtcaccat gaaatcattt gaaaatgcaa ttatggttca
     1681 tgcagcaatt tctggaagta ctaattcact gttacactta ccagctattg caagtgagtt
     1741 tggaattgat ctaaatgctg atttatttga tcagatgcat caaaatgcac attatcttct
     1801 tgatattcgt cctgcaggaa aatacccggc tgaatatttt tactatgcgg gtggtgttcc
     1861 agcggtcatg gaagcaatta aagagaatct taatttagat gtaatgacag ttactggtaa
     1921 aactttaggt gaaaatttgg aagaattaaa aaataatggt tactatgaaa attgccaaac
     1981 atttttggat aagatcaatc taaaaagcac tgatattatt agaccatatg atgatcccat
     2041 tggaaaagat ggaacaattg ccattttaaa agggaattta gcaccagatg gagcagtaat
     2101 caaacattca gcatgtcctc aagaaatgat gcaagctgtt cttagggccc gcccatttga
     2161 tagcgaagag gcagccattg acgcgatcat caaaaaacaa atcaaacccg gtgatgcagt
     2221 atttattcgt tacgaaggac ctaaaggtag tggaatgcct gaaatgttct atactggtga
     2281 agccattagt tctgatcagg aactagccaa gagtattgca ctaattactg atggacgttt
     2341 ttctggagct agcaaaggac cagttattgg tcatgtctca ccagaagcta gtgagggagg
     2401 accaattgct ttaatcgagg aaaatgactt gatcaaaatt gatgttcctc aacgtcgatt
     2461 agaaattatt ggaattgaag gaaaacaacg tacctcacaa gaaattacaa aaatattaaa
     2521 ggagcgccat gctaaatgga aaccaaaaga accaaaatat aaaaatggaa ttt
//
LOCUS       DAOMTA010000425         2572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1809_length_2572_cov_2.237187, whole genome shotgun sequence.
ACCESSION   DAOMTA010000425 DAOMTA010000000
VERSION     DAOMTA010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_2572_cov_2.237187"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..610)
                     /locus_tag="PLK68_10875"
     CDS             complement(74..610)
                     /locus_tag="PLK68_10875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM90946.1"
                     /translation="MSLYVTRHGQTNYNVNNLVCGISPAALTTDGIEQAKELGRQLKS
                     IKYDFLYVSPLQRAIDTADYANVEGLEVIIEPRISEINFGIYEGVHRDDPGFIANKHN
                     LAIRYPNGESFIELCKRVYEFLDEIKEQATKSNVLLVCHGAVCRAINTYFNEMSNDDI
                     FYYQTENCQLLKYDYLSR"
     gene            complement(788..2005)
                     /locus_tag="PLK68_10880"
     CDS             complement(788..2005)
                     /locus_tag="PLK68_10880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM90947.1"
                     /translation="MQRFTLPRDLYHGKGSLEALKSLEGKKAIICVGGGSMKRNGFLD
                     RAIAYLEEAGMQVKLFEGIEPDPSVETVMKGAAVMEEFQPDWIVAMGGGSPIDAAKAM
                     WIKYEYPEITFEEMCKVFGIPKLRKKAHFCAIPSTSGTATEVTAFSIITDYEKGIKYP
                     IADFEITPDVAIVDPDLAETMPLKLVAHTGMDAMTHAIEAYVSTANCNYTDPLAIHAI
                     EMIQANLVKSYNGDMGARDDMHDAQCLAGQAFSNALLGIVHSMAHKTGAAFVDQGGHI
                     IHGAANAMYLPKVIAFNAKDETAKKRYGVIADYMHLGGNSDDEKVALLIAYLRKMNDE
                     LNIPHSINHYGADGLPADTGFVSEDVFLARVDNIAALAIEDACTGSNPRIPTQEEMVE
                     LLKACYYDSEVDF"
     gene            complement(2095..>2572)
                     /locus_tag="PLK68_10885"
     CDS             complement(2095..>2572)
                     /locus_tag="PLK68_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90948.1"
                     /translation="EESYFLHLLETKELNFEKLYQFNYQLLFSNENPLSFKEIIYLSD
                     LETQIEITHGDTALPSLPIVATNRNDETRHSKKEISVYERASQFELLNHLPHTFMWVS
                     PIPQEVLKRFNLVTKECQENKMSYHDLLITRKGYRLSKEDEGFIRAIKCSIKEINI"
BASE COUNT          814 a          514 c          354 g          890 t
ORIGIN      
        1 atttttatca aaagggctgg atcgacctag cctatcgtaa aatcattatt gttgaccatg
       61 atgagttaag caattatcta gataaataat catatttaag caattggcaa ttttccgttt
      121 gataataaaa aatatcatcg ttgctcatct cattaaaata cgtattaatt gcccgacaaa
      181 cagcaccatg acacactaat aaaacattac ttttggttgc ctgttctttg atttcatcaa
      241 gaaattcata gacccgcttg cataattcaa taaacgattc accattagga taccgtattg
      301 ctaaattatg tttattagca ataaaaccag gatcatcacg atgtaccccc tcatagatac
      361 caaaattaat ttcactgatt ctcggttcaa taatcacttc taaaccttct acattagcat
      421 agtcagcagt atcaatagca cgttgtaacg gtgatacata taaaaaatca tatttaattg
      481 attttaattg tcgacccaat tctttggctt gttcaattcc atctgttgtc aaagcggctg
      541 gactaattcc gcaaactaaa ttattaacat tataatttgt ctgtccgtgt ctcgttacat
      601 aaagactcat ttcattcacc tgctttactt ttatcactgt actcacaatg attttttctt
      661 aactaacggt tattctacta caaataagtc tctttattca actaattata gtatattctt
      721 ttgataaaat aaaagaaaag acaaggccct tgcttccttg tctaaatcgc taaaaagttt
      781 ttatttttta aaaatctact tcgctgtcat aatagcaagc ttttaacaac tcaaccattt
      841 cttcttgagt tggaattctt ggattagaac ctgtacaagc atcttcaatt gctagtgctg
      901 caatattatc aactcgtgcc aagaatacat cttcgctaac aaaaccagta tctgctggta
      961 atccatcagc tccataatga ttgattgaat gtggaatatt taattcatca ttcattttgc
     1021 gtaagtatgc aattaataaa gctacttttt catcatcact atttccacct aaatgcatat
     1081 aatcagcaat tacaccataa cgttttttag ctgtttcatc ttttgcatta aaggcaataa
     1141 cttttggcaa gtacattgca tttgctgccc cgtgaataat gtgaccacct tgatcaacaa
     1201 aagctgcacc agttttatgt gccattgaat gcacgattcc taatagcgca ttagagaatg
     1261 cttgtcctgc taaacattgg gcatcatgca tatcgtcacg agcacccata tccccattgt
     1321 atgatttaac taaattagct tgaatcattt caatggcatg aatcgctaat ggatcagtgt
     1381 aattacaatt cgcagttgaa acatatgcct caatcgcatg cgtcatcgca tccataccgg
     1441 tatgcgcaac tagcttaagt ggcattgttt cagctaaatc aggatcaaca attgctacat
     1501 ctggtgtaat ttcaaaatca gcaattggat atttaattcc tttttcataa tctgtaatga
     1561 ttgagaatgc tgttacttct gtagcagttc cactagttga tggaattgca caaaaatgtg
     1621 ctttttttct tagtttagga ataccaaaaa ctttacacat ttcttcaaat gtaatttccg
     1681 gatattcata cttaatccac attgctttag cagcatcgat tggtgaacca ccacccatag
     1741 ctacgatcca atcaggttgg aactcttcca tgactgcagc acctttcatc actgtttcca
     1801 cagatggatc aggttcaatc ccttcaaata gttttacttg cattccagct tcttctaaat
     1861 aagcgatagc acgatcaaga aaaccatttc ttttcatact tccgccaccg acacaaataa
     1921 tcgctttttt tccttctaaa gattttaaag cttctaacga accttttccg tgatacaaat
     1981 ctctaggtaa tgtaaatctt tgcatcagac cacctccata accaaattat agcgcttaca
     2041 tatttagtta tgaagagaca aaaaaggaat agccctatcc ctttttatgt atacctagat
     2101 attaatttct ttaatactac acttgattgc tctaataaag ccctcatctt ctttcgacaa
     2161 acgatatcct tttctcgtaa tcaacagatc atgataactc attttatttt cttgacactc
     2221 cttagtaact aaattaaaac gttttaaaac ttcttgagga atcggtgaca cccacataaa
     2281 agtatgaggt agatgattta ataactcaaa ttgactagcc cgttcatata ccgaaatttc
     2341 ttttttacta tgacgtgttt catcattacg gttggttgct acgatcggta atgatggtaa
     2401 agctgtatcc ccatgtgtaa tttctatttg ggtttctaga tcagatagat aaataatctc
     2461 tttaaatgat aacggatttt catttgaaaa taataattga taattaaatt gatatagttt
     2521 ttcaaaattt agttcctttg tttctagtaa atggagaaaa taactctctt ca
//
LOCUS       DAOMTA010000426         2572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1810_length_2572_cov_2.187922, whole genome shotgun sequence.
ACCESSION   DAOMTA010000426 DAOMTA010000000
VERSION     DAOMTA010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1810_length_2572_cov_2.187922"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLK68_10890"
     CDS             <1..991
                     /locus_tag="PLK68_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HRM90949.1"
                     /translation="KIIAFEVRSDEMADFEIMNNSNNFEITYYKEYLCQKNLSLLNGY
                     DGIAFLGESKINHEILDAIAKAGIGVIATRTIGYDHIDIEYAKELGIKVCNSSYGPEG
                     VADFTVMLMLLSLRHYKKALWLGQVNDYSLQGLEGREMKDLTIGIMGTGRIGQAVLKN
                     LSGFGARLLAYDIHQNETAKIYATYVDLEQFYQECDIISLHMPYLKSTHHLINDQTIS
                     KMKDGIIIINCARGQLCNTESLIRGIENKKIGALGLDVVEGEEGIYHQDMRTDIIKNK
                     NMAYLRQFPNVVMTQHLAFYTDAAVSSMVQLSLNGLWACYNDLDWDNELTKDL"
     gene            1154..2443
                     /gene="eno"
                     /locus_tag="PLK68_10895"
     CDS             1154..2443
                     /gene="eno"
                     /locus_tag="PLK68_10895"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM90950.1"
                     /translation="MSKIKSVYAREVLDSRGNPTVEVEVTTECGAFASAIVPSGASTG
                     VHEAVELRDGDKNRYLGLGTEKAVKNVNEIIAKELIGKEVTNQREIDETMIALDGTPN
                     KGKLGANAILGVSLAIAKCAATSLDLPLYRYIGGANAYVLPTPMMNIINGGAHADNNV
                     DFQEFMIMPVSAPTFKEAIRMGAEVFHALKAVLKGKGLNTAVGDEGGFAPNLASNEDA
                     IKTILEAVEKAGYKPGEDIKLAMDVASSEFYQDGKYVLPGENNKSFTSKELVDFYAEL
                     CSKYPIISIEDGLDQDDWEGWDYLTEKLGDKVQLVGDDFFVTNTKRLEEGIKRGVANS
                     ILIKVNQIGTLTETLEAIEMAQKANYTAVISHRSGETEDTTIADIAVATNAGQIKTGS
                     ASRTDRIAKYNQLIRIEDRLGKQSKYSGLSGFYQLNK"
BASE COUNT          923 a          320 c          543 g          786 t
ORIGIN      
        1 gaagattatt gcatttgaag tacgaagtga cgagatggca gattttgaaa ttatgaataa
       61 ttcaaataac tttgaaataa cttattataa ggaatatcta tgccaaaaaa atttatcttt
      121 attgaatgga tatgatggaa ttgctttttt aggggagagt aagatcaatc atgaaatttt
      181 agatgctatt gctaaagcag gaattggagt tattgcaaca agaacaatag gatatgatca
      241 tattgatatc gaatatgcca aagaattagg aataaaggtt tgtaattcta gttatggacc
      301 ggaaggagtt gcagatttta ctgtcatgct aatgctgtta tcgttacgtc attataaaaa
      361 agctttgtgg ttaggacagg taaatgatta ttctttacaa gggctagaag ggcgagagat
      421 gaaagattta acgataggaa ttatggggac cggacgaata ggtcaagcag ttttaaaaaa
      481 cttatccggc tttggagctc gtttattagc ttatgatatt catcaaaatg aaaccgctaa
      541 aatatatgca acttatgtcg acctagagca gttttatcaa gaatgtgaca ttatttcttt
      601 gcatatgccg tatcttaaaa gtacgcacca tctaattaac gatcaaacaa taagtaaaat
      661 gaaagatgga ataatcatta ttaactgtgc tcgggggcaa ttatgcaata ccgagtcttt
      721 aatcagggga atcgaaaata agaagattgg ggctttagga cttgatgtgg ttgaaggtga
      781 agaaggaatt tatcatcaag atatgcgtac tgatataatt aaaaataaaa atatggcata
      841 tttacgtcaa tttcctaatg ttgtcatgac acaacattta gctttttata ctgatgcagc
      901 tgttagcagt atggttcaat tatcacttaa tggtctatgg gcttgttata atgatctgga
      961 ttgggataat gagttaacaa aagatttgta atcgcagaaa tttctgtgat ttttttaatg
     1021 ctttttactt gcacataagg ttagtatgtg ctaaccttat tgtagttagg taaaactaac
     1081 ttcgtgtgaa taaatgattc tttctaacct ggcttagtca cccaggttaa ggaatcatga
     1141 aggaggaaaa caaatgagta aaattaaaag tgtatatgcc cgtgaggtct tggattcacg
     1201 tggtaatcct actgtagagg tagaagtaac tacagaatgt ggagcattcg ctagtgcgat
     1261 agtaccaagt ggagcttcaa caggtgttca tgaggcagtt gaattacgtg atggtgataa
     1321 aaaccgttat ttaggattag gaacagaaaa agcagtaaaa aatgttaatg agattattgc
     1381 taaagaatta attggtaaag aagtaacaaa tcaaagagaa atcgatgaaa cgatgattgc
     1441 tttggacgga acaccaaata aaggtaagtt aggtgcaaat gcaatcttag gtgtatcttt
     1501 agctattgct aaatgtgctg cgacgagctt agatttacca ttatatcgtt acattggagg
     1561 agctaatgct tatgtattac ctacaccaat gatgaatatc attaatggtg gagctcatgc
     1621 agataataat gtagattttc aggaatttat gattatgccg gttagcgctc caacatttaa
     1681 agaagcaatc agaatgggtg cggaagtatt ccatgctttg aaagccgttt taaaaggaaa
     1741 aggattaaat acagcagttg gtgatgaggg aggctttgcg ccaaatttag cttctaatga
     1801 agatgctatt aaaactattc tagaggcagt agaaaaggct ggctataaac caggtgaaga
     1861 tattaaattg gcaatggatg ttgcaagcag tgagttttac caagatggta aatatgtatt
     1921 accaggagaa aataataaat catttacttc aaaagaatta gttgattttt atgctgaatt
     1981 atgttctaaa tatccaatta tctcaattga agatggttta gatcaagatg attgggaagg
     2041 ttgggattat ctaactgaga agttagggga caaagtacag ttagtcggtg atgatttctt
     2101 tgtaactaat actaaacgtc ttgaagaagg aattaaacgc ggtgtagcta attcaatctt
     2161 aattaaagta aatcaaattg gtactttaac agaaacttta gaagctattg aaatggccca
     2221 aaaagcaaat tatacagcag ttatctctca tagatcagga gaaacagaag atacaactat
     2281 tgctgatatt gcggtagcaa caaatgcggg acaaattaaa actggctctg catcaagaac
     2341 agaccgaatt gctaaatata atcaattaat tagaattgaa gatcgtctag gtaaacaaag
     2401 taaatattct ggattatcag gattttacca attgaataaa taatttggat aaattttata
     2461 aaaactattg catagtcagt tagtctgtgc taacatatag atagttagtt taagctaact
     2521 agagaaaagg agaaatgtat gaaagtagta tttgcatcaa gaacaggtaa tg
//
LOCUS       DAOMTA010000427         2568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1814_length_2568_cov_1.990052, whole genome shotgun sequence.
ACCESSION   DAOMTA010000427 DAOMTA010000000
VERSION     DAOMTA010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_2568_cov_1.990052"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>705
                     /locus_tag="PLK68_10900"
     CDS             <1..>705
                     /locus_tag="PLK68_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRM90951.1"
                     /translation="GFSVNIYRYIKKIVQEDIADLNELRAPIGQYFKQGFGVLAVGLI
                     CVLGTLAFVVPGIILGLGLSMTPYLLANYPSLSIFEAITTSWKMMQGKKMKCFVLFFS
                     FYGWILLSTVTLGILFIWLLPYMTLTFNKFFLENENEFYGVVNSNNNVSSNDIEEFAL
                     LNNLKLDTRNNVYGMFNDYPVVVMFGSANNDLIITIDTIGEDRTALDEYFNNLRTILT
                     NIKTINYSNGTITLSA"
     assembly_gap    706..715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <716..1330
                     /locus_tag="PLK68_10905"
     CDS             <716..1330
                     /locus_tag="PLK68_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90952.1"
                     /translation="EVYEILNRITLKLRDLGFLPSCGTCHINKPTSFYEYHGQLMNMC
                     DDCRDAISTQAEEIVEDSSRGIIGAVAGALIGGVLWALVYQIGFIVAFLGYLIVFLAI
                     NGYQRMAGKISKKGLIISIICRVLVLFFAESISLGLKIKDLMQLQSIFTAFSYIPYFL
                     SFSEIFAIVVRDIVLGLIFMGLGSWQYIYKIKKSLDEENLNKLD"
     gene            complement(1327..1980)
                     /locus_tag="PLK68_10910"
     CDS             complement(1327..1980)
                     /locus_tag="PLK68_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM90953.1"
                     /translation="MKNQIIFRPIIKKDYLAIEKIIREAWHYDEFCTSKIAKLLSKIF
                     LSSCLSNQTFTLVALLKEQPIGIIMGKNIKTHKCPFKYRVKQGFNIFNLLIRKERRKT
                     AKIFKAVNNIDKVLLQQTNQDYQGELSFFAIDAQYRGLGLGKELFNLLISYMQEQQIN
                     RFYLYTDTSCNYHFYEHLGMMRRVEQTHCFKINNEKNVMHFFIYDYLCKKTVSTETV"
     gene            2051..>2568
                     /locus_tag="PLK68_10915"
     CDS             2051..>2568
                     /locus_tag="PLK68_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM90954.1"
                     /translation="MLKNESLKLTTAQFAKLHKINKRTLHYYDEIDLFKPKFKGENNY
                     RYYDYFQSIELENILMLKQLDMSISEIKSYLNNPNVNDFVNIADDKILKIEQDIRRLK
                     QTKKVLEIKKNQLLKSSRVTDFEIEIVERQDEYLLVSNEPFIQYDVKEILEYLQQAWN
                     IEQYKVGCGSYI"
BASE COUNT          882 a          307 c          435 g          934 t
ORIGIN      
        1 tggtttttca gtcaatatat atcgttatat caaaaaaatt gtgcaggagg atattgctga
       61 tcttaatgaa ttgagagcac ctatagggca atattttaaa cagggatttg gtgttctggc
      121 tgttggactt atttgtgttt taggaacatt ggcatttgtt gttccaggaa ttattttggg
      181 attagggtta tcaatgacac cttatctttt agcaaattat cctagtttat caatttttga
      241 agcaattaca acaagctgga aaatgatgca aggaaagaaa atgaaatgtt ttgtcttatt
      301 ttttagtttt tatggctgga ttttattaag tacagtaaca ctgggaatat tatttatttg
      361 gttattgcca tatatgacat taacatttaa taaattcttt ttagaaaacg agaatgaatt
      421 ttatggtgta gttaacagta acaataatgt aagtagtaat gatattgaag agtttgcttt
      481 attaaataat ttgaaacttg atacacgtaa taatgtatat ggaatgttta atgattatcc
      541 agtagtagtg atgtttggga gtgctaacaa tgacctaatt attacaatcg atactattgg
      601 ggaagatcga actgctctag atgaatattt taataattta agaacaatat taacaaacat
      661 taaaactatt aattatagta atggaacaat taccttaagt gctttnnnnn nnnnngaagt
      721 atatgagatt ttaaatcgaa ttactttaaa gttaagagat ctgggcttcc ttccaagttg
      781 tggaacttgt catattaata aaccaacttc attctatgag tatcatggtc aattgatgaa
      841 tatgtgtgat gactgtagag atgcaatcag tacccaagct gaagaaatcg ttgaggacag
      901 tagccgagga atcattggtg ctgtggctgg tgctctaatt ggtggtgtgc tttgggcatt
      961 ggtgtatcaa atcggtttta tcgtcgcatt cttaggttat ttaattgttt ttttagcgat
     1021 aaatggttat cagcgaatgg ctggtaaaat ttctaaaaaa ggattaataa tttcaattat
     1081 ttgtcgcgtt ttggtactgt tttttgcaga atcaatcagc ttagggctaa aaattaaaga
     1141 tctgatgcaa ttacaaagta tttttactgc attttcatat attccttact tcctttcatt
     1201 cagtgaaatc tttgctattg tagttagaga tatagtcctt ggattaatct ttatgggatt
     1261 aggaagctgg caatatattt ataagattaa aaagtctttg gatgaagaaa atctaaataa
     1321 actcgattaa acagtttcag tagaaactgt ttttttgcac aagtagtcat aaataaaaaa
     1381 atgcattacg tttttttcat tgttgatttt aaaacagtga gtttgttcaa ctcggcgcat
     1441 catccctaga tgctcataga aatgataatt acagcttgta tcagtgtata aataaaatct
     1501 attgatttgt tgttcttgca tataactaat cagtagatta aataattctt ttccaagacc
     1561 taatccccga tactgtgcat caattgcaaa gaaagatagc tcgccttggt agtcttgatt
     1621 agtttgttgt aacaaaactt tatcaatatt attgacagcc ttaaatattt tagctgtttt
     1681 tcgtctctct tttcttatta agagattaaa aatattgaat ccttgtttaa ccctatattt
     1741 aaagggacat ttatgtgttt tgatattttt tcccataata atgccaatag gctgttcctt
     1801 tagcaaggca acaagcgtaa aagtttgatt gcttaaacag ctgcttaaga aaatttttga
     1861 tagtaattta gctattttgg aagtacaaaa ctcatcatag tgccatgctt cacgaataat
     1921 tttttcaatt gctaaatagt cttttttaat aattggtcta aaaataattt gatttttcat
     1981 actattctcc tttatttaat atataattat cataaagtct acggttactg taaagtcaag
     2041 gaggaacata atgttaaaaa atgaatcatt aaaactaact actgcacaat ttgctaaatt
     2101 acataaaatt aataagcgaa cgctacatta ctatgatgaa attgatttat ttaaaccaaa
     2161 atttaaaggt gaaaataatt atcgatatta cgattatttt caaagtattg aattagaaaa
     2221 tattctaatg ctaaagcagt tagatatgag tatcagcgaa attaaaagtt atctaaataa
     2281 tcctaatgtt aatgattttg taaatattgc tgatgataaa atactaaaaa tagaacaaga
     2341 tattcgtcgt ttaaaacaaa caaaaaaggt attagagata aaaaagaatc aattgttgaa
     2401 gagttcacga gttactgatt ttgaaatcga aatcgttgag cgccaagatg aatatttatt
     2461 agtttcaaat gagcctttta ttcaatatga tgttaaagaa attttagaat atttgcagca
     2521 ggcttggaat atagaacaat ataaagtagg gtgtggcagt tatattag
//
LOCUS       DAOMTA010000428         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1820_length_2564_cov_2.003189, whole genome shotgun sequence.
ACCESSION   DAOMTA010000428 DAOMTA010000000
VERSION     DAOMTA010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_2564_cov_2.003189"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLK68_10920"
     CDS             complement(<1..756)
                     /locus_tag="PLK68_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM90955.1"
                     /translation="MAEYKGAGDTVTINDVLEVCGKYITNQESIDLINQAYDFIMEKH
                     EGQKRKSGEPYTIHLIWVAYILATLQTGPATIAAGLLHDVMEDCGVSREEMVERFGEE
                     ITTLVEGVTKIGKMPFKDESDVYAENHRKIYIAMAKDIRVILIKFADRLHNMRTLQFM
                     PPHKQKRISRETLEVYTPIAHRLGINDIKTELEDLSLSYLDPVAYSEISKLLATKQEE
                     RKESVAKMMASVKQILDDNSYQYRILGRAKSIYS"
     gene            complement(801..1319)
                     /locus_tag="PLK68_10925"
     CDS             complement(801..1319)
                     /locus_tag="PLK68_10925"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRM90956.1"
                     /translation="MDLTKYVASIENFPEDGIIFRDVTPLMADKEAFKETIDRFVEWT
                     KSLDTNVDIVAGPEARGFLFGCPVAYNLNAGFVPVRKPGKLPRETVSETYALEYGTNE
                     VHMHADSIKPGQNVIIVDDLLATGGTVEATVKLVEKLGGKVVGIAFLIELEGLKGRDK
                     LQGYNIYSILKY"
     gene            complement(1383..>2564)
                     /locus_tag="PLK68_10930"
     CDS             complement(1383..>2564)
                     /locus_tag="PLK68_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRM90957.1"
                     /translation="VDVIVTDHHNYDDELPDAFSIIHTKISPDYPYKEISGGFVAYKL
                     ASALLKKHDKYLFSLAAITTISDMMPLLDENRALVKRALEFMKENKYPQLELLLGSNQ
                     TYSVTSIGFIIAPKINSFGRLSEIINPNHLVKYFRSDASIGILKAVSEKAIEVNAKRQ
                     ELTNKQYQSVLTMIDPNEQFLYAYQNEIHEGIIGLVAGKYTRQYNRPSFVMTFDSEKN
                     LYKGSARGIEGIPLNKIFENVQDLLESYGGHALAGGFSVGSDKVEELKEKLELYLNKQ
                     LKDYTPPLVDVIEVTGNEISKVSVKQLELLEPLGNGNEEMRFFIKDLPVSKVTSLSNG
                     KHIRFDLSLPQVKAQALFFNHGDLFEQLKDTKMINAVGKFNINVFNNIESVNLIIEDI
                     K"
BASE COUNT          815 a          512 c          312 g          925 t
ORIGIN      
        1 actatagatt gatttagccc gtcccaagat acgatactga tagctattat catccaatat
       61 ttgtttaacc gatgccatca tttttgcaac tgattctttc cgttcttctt gtttagtcgc
      121 taataatttt gatatctcgc tatatgcaac aggatcaaga taagataggc ttaaatcctc
      181 taactccgtt ttgatatcat tgattcctaa acgatgagca attggtgtat aaacttctaa
      241 cgtttctcga gaaattcttt tttgtttatg aggtggcata aattgtaatg ttcgcatatt
      301 atgaagtcga tctgcaaatt taatcaaaat aacgcgaata tcctttgcca tagcaatata
      361 aattttacga tgattttcag cataaacatc agattcatct ttaaatggca ttttaccaat
      421 cttggtaacc ccttcaacta acgtagtgat ttcctcacca aaacgttcta ccatttcttc
      481 ccgagatact ccacaatctt ccatgacatc atgcaaaagt cctgccgcaa tagtagcggg
      541 tcccgtttgt aacgttgcca aaatatatgc tacccaaatc aggtgaatcg tatacggttc
      601 accgcttttg cgtttttgcc cctcatgctt ctccataata aaatcgtatg cctgattaat
      661 taaatcaata ctttcttgat ttgtaatata tttaccacac acttcaagaa catcattaat
      721 tgtaactgta tcgccagcac ctttatattc agccataatg tcaccccctc ttattattaa
      781 aaaagagatt taatctcttt ttagtatttc aatattgaat agatattata cccttgaagt
      841 ttatcacgtc cttttaatcc ttcaagttcg attagaaaag caattcctac tacctttcca
      901 cctaattttt caactaattt tacagttgct tcaactgttc caccagttgc taataagtcg
      961 tctacaataa taacattttg ccctggttta atactgtcag catgcatgtg cacttcatta
     1021 gttccatatt ctaaagcata agtttcactt actgtttcac gaggtaattt acccggtttt
     1081 ctaaccggaa caaaccctgc atttaaatta tatgcaacgg gacatccaaa taaaaaacct
     1141 cgagcttctg gtccagcaac aatatcaaca ttagtatcca gacttttagt ccactcaaca
     1201 aatcggtcaa tcgtttcttt aaaagcttcc ttatcagcca tcaacggtgt tacatctctg
     1261 aaaataatac catcttcagg aaagttttca atacttgcaa catacttagt taaatccatt
     1321 ctatttcctc cacataatat cctatgatat ttaattataa catttttata ctttaattta
     1381 aactatttaa tatcttcaat aattaagttg acagattcaa tattattaaa aacattaata
     1441 ttaaacttac caactgcatt gatcatttta gtatctttta attgttcaaa aagatctcca
     1501 tgattaaaaa ataacgcttg tgctttgact tgcggtaatg acaaatcaaa acgaatatgt
     1561 ttaccattcg ataacgacgt aactttactt actggtaaat cttttatgaa aaaacgcatt
     1621 tcttcattgc cattgcctag tggctctaat aactctaatt gttttacact aactttacta
     1681 atctcatttc ctgtaacttc aattacatcc actaaaggag gagtataatc ttttaattgt
     1741 ttatttaaat acaattctaa tttttctttt agctcctcaa ctttatcact acctacacta
     1801 aatccacctg ctaaagcatg accaccataa ctttctaaca aatcttgaac attttcaaaa
     1861 attttattca acggtattcc ttcaattcca cgagcactgc ctttataaag atttttttca
     1921 ctatcaaatg tcatcacaaa gctaggtcgg ttatattgtc gagtatattt tcctgccact
     1981 aaaccaatga ttccttcgtg tatttcattt tgataagcat ataaaaattg ctcattcggg
     2041 tcaatcattg ttaaaacgct ttgatattgt ttatttgtta attcttgtct tttagcattt
     2101 acttcaattg ctttttcact aactgctttt aatattccaa ttgaagcatc acttctaaaa
     2161 tatttaacga gatgattagg attaataatt tcacttaagc gaccaaatga attaatcttc
     2221 ggggcgatga taaacccaat actagtaaca ctataggtct gattactccc caataacaat
     2281 tctaattgag gatatttatt ttctttcata aattccaatg ctctttttac caatgctcga
     2341 ttttcatcaa gtaatggcat catatcagaa attgttgtaa tagctgccaa tgaaaataag
     2401 tatttatcat gcttttttaa taatgcactt gctaatttat aagctacaaa accacccgag
     2461 atttctttat aaggataatc gggtgagatt tttgtatgaa taatagaaaa agcatctggt
     2521 aattcatcat cataattatg gtggtcagta acaataacgt ccac
//
LOCUS       DAOMTA010000429         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1823_length_2562_cov_3.244116, whole genome shotgun sequence.
ACCESSION   DAOMTA010000429 DAOMTA010000000
VERSION     DAOMTA010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_2562_cov_3.244116"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /gene="pglZ"
                     /locus_tag="PLK68_10935"
     CDS             <1..1100
                     /gene="pglZ"
                     /locus_tag="PLK68_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HRM90958.1"
                     /translation="SQQAIYPTITKFGMAALLPKKALSLENKNGSLKVLVDGNTSEMS
                     DRDGILKATNSNSVAVKYKDLVLMKRDDRRNVIKGMDVVYIYHDTIDNTSHHDEQGVF
                     AACDTAIEELKNLVKIITGELNGLNVIITSDHGFLYTYQSLNEDDKMERSSFKKNIIE
                     QGRRYVITDDQANPDYLVPVKGFYNNNGYQAFAPRENIRIKGAGAMNFVHGGMSLQEM
                     VVPVIKYKYLRSGYKSYERNKDKYDAKPVTVSLLSSSRKISNMIFNLSFYQKEAVANN
                     YVACTYDVFMTDSQGNVVSDKQKIVADRVSQSAKDREYRCTFNLKQQTYNRSESYYLV
                     IQDEDGLQVPIKEEMTIDIAMSFDDFDFFDI"
     gene            1119..2039
                     /locus_tag="PLK68_10940"
     CDS             1119..2039
                     /locus_tag="PLK68_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRM90959.1"
                     /translation="MIFDNLSHDERVNYLIKPILKVLQDAGGQLKRSEIKEKLMEEDE
                     DIAEYASMKKKSKKTGNEYKEFDFKFNFAIKDLSFAKFLVFEKRNPTITLTDKGINAD
                     LSNFDVQKEVIDISNKCWAEVINQNKDKKPNVEKEEETIVNSDISLEEEYDQDFKTRL
                     LEAISKMSPKKFELFSRILLKKMGVEFTEIGVQVSNDGGIDGYGYHHDSSDFRTTRVV
                     IQCKRYNAGPVSEPEINQFLGAMSKFNADYGVFITNSRFTKAAKTAALQGHPITLIDG
                     NDLVDLVKKYEIYIKPITTYELLDFYDEEK"
     gene            2066..2133
                     /locus_tag="PLK68_10945"
     tRNA            2066..2133
                     /locus_tag="PLK68_10945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2096..2098,aa:Ser,seq:tga)
     gene            2163..>2562
                     /locus_tag="PLK68_10950"
     CDS             2163..>2562
                     /locus_tag="PLK68_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HRM90960.1"
                     /translation="MDIELSNREIIKDKLMNNFGGKFVRKDLTKKIKEGANVPVYVLE
                     FLLGQYCSTEDETVIEEGVNKVKKILSDNFVRPDEAQKILSVLKQRGSYSVIDKITVA
                     LNIRNDVYEAEFSNLGIKNIPIEEDYPAKYD"
BASE COUNT         1002 a          300 c          480 g          780 t
ORIGIN      
        1 atagccaaca agctatatat ccaacaatta ctaagtttgg tatggcagct ttacttccta
       61 agaaagctct atctttagaa aataaaaatg gttcattaaa agtattagta gatgggaata
      121 cttcggaaat gagcgataga gatggaatct taaaagctac aaattctaat agtgtagcag
      181 ttaaatacaa agatttagtt ttaatgaagc gtgatgacag acgaaatgtc attaaaggaa
      241 tggatgtcgt ttatatttac catgacacta ttgataatac tagtcatcat gatgaacaag
      301 gtgtattcgc tgcgtgtgat acagctattg aagaattaaa gaatttagta aaaataatta
      361 cgggtgaatt aaatggctta aatgtcatta ttacatctga tcatggtttc ttatatacat
      421 atcaatcatt aaatgaagat gataagatgg aacgttcatc attcaagaag aatattattg
      481 aacaaggtag aaggtatgta ataacggatg accaggcaaa tcctgattat ttagtaccag
      541 ttaaaggttt ctacaacaat aatggttatc aagcatttgc accaagagaa aatattagaa
      601 tcaaaggcgc aggtgctatg aactttgttc atggtggaat gagcttacaa gaaatggtag
      661 taccagttat caaatataaa tatttgagat ctggatataa atcatacgaa agaaataaag
      721 ataagtatga tgcaaaacct gtaacagtat cattattatc ttcaagtaga aagattagta
      781 acatgatttt taacttaagt ttctaccaaa aagaagctgt tgctaataac tatgttgcat
      841 gtacatatga tgtatttatg acagattcac aaggtaatgt agtcagtgat aagcaaaaga
      901 ttgttgctga tagagtgagt caatctgcta aagatagaga atacagatgt acttttaact
      961 taaaacaaca aacatataat agatcagaat catattactt agtgattcag gatgaagatg
     1021 gattacaagt accaattaaa gaagaaatga caattgatat tgcaatgtca tttgatgatt
     1081 ttgatttttt tgatatctag atagagggag gtgaaaccat gatatttgac aacttatcac
     1141 acgatgaaag agttaattac ttaataaaac caattctaaa agtattacaa gatgcaggtg
     1201 gtcaattaaa acgttctgaa attaaagaaa aattaatgga ggaagatgaa gatattgctg
     1261 aatatgcatc aatgaaaaag aagtctaaaa agacaggaaa tgaatacaaa gagtttgatt
     1321 ttaaattcaa cttcgctatt aaagacttat cgtttgctaa attcttagtg tttgaaaaaa
     1381 ggaatcctac gattacttta acagataaag gaattaatgc agatttaagc aattttgatg
     1441 ttcaaaaaga agtaattgat atttctaata aatgttgggc agaagtcatt aatcaaaaca
     1501 aagacaagaa gccaaatgtt gaaaaagagg aagaaacaat tgtaaatagt gacatcagtt
     1561 tagaagagga atatgatcag gattttaaaa ccagattatt agaagcaatt tctaaaatgt
     1621 cacctaaaaa gtttgaattg ttttcacgta tacttttaaa gaaaatgggt gttgaattta
     1681 ctgaaatagg tgttcaagtt tctaatgatg gtggaatcga tggttatggt tatcaccatg
     1741 attctagtga ctttagaaca acaagagtgg ttatccaatg taagagatat aatgctggtc
     1801 cagtatcaga accagagatt aatcaattct taggggctat gagcaagttt aatgctgatt
     1861 atggggtatt tatcactaat agccgattta ctaaagcagc taaaacagca gcattacaag
     1921 gacatcctat cacattaatt gatggtaatg atcttgtgga tttagttaag aaatatgaga
     1981 tttacattaa acctataact acatatgagt tgttagattt ttatgatgaa gaaaagtaat
     2041 agtggacgac actattactt tatatgacga tatagcacaa cgggagtgca ttacatgaag
     2101 taaagattgc tggttcgact ccagcagttg tcatcaataa ggaaaaatgg aggtattgaa
     2161 taatggatat cgaactttca aatagagaaa taattaaaga taaattaatg aataactttg
     2221 gtggtaagtt tgtacgtaaa gatcttacaa aaaagattaa agaaggagca aatgtaccag
     2281 tatatgtact tgaattctta ttaggacaat actgtagcac agaagatgaa accgttattg
     2341 aggaaggagt taataaggtt aagaagatac tttccgataa ctttgttcgt ccagatgaag
     2401 ctcaaaaaat cttatccgta ttaaaacaaa gaggatcgta ctcagtaatt gataagatta
     2461 ctgttgcttt aaatattaga aatgatgttt atgaagctga gttttcaaat ttaggaatta
     2521 agaatattcc tattgaagaa gattatccag caaaatatga tc
//
LOCUS       DAOMTA010000430         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1825_length_2559_cov_2.098642, whole genome shotgun sequence.
ACCESSION   DAOMTA010000430 DAOMTA010000000
VERSION     DAOMTA010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_2559_cov_2.098642"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLK68_10955"
     CDS             <1..1045
                     /locus_tag="PLK68_10955"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM90961.1"
                     /translation="ADFVSTQIRVGGNKARIKDEKIPLKYGLIGQETTGVGGMFNAFR
                     VIPVMIEIAKDVEKYCPDAWIINYSNPTGLVTEALNKVCNVKVAGLCAGGMRPRWWAA
                     EALNIPEEKIYYDYLGLNHMNFSYNMTIDGRPITDEEFSKVIKKCTTVSQEWMETLHL
                     IPSQYTQYYFHTKERVQQLKEQDKTRGEAILELEKQIFREYADELNNQKPKTLAKRGG
                     GGYSKVAIGVMDAIYNDSGKWMVINVANNGTVRFLDDDAVIETACYVSKNGMKPLTIA
                     HYPKAVIGLISAVKTYESLAVEAAITGNKKLALEALVAHPLVRDYDVAKPLLEEMLEA
                     HKEYLPQFYHHGK"
     gene            1047..2078
                     /locus_tag="PLK68_10960"
     CDS             1047..2078
                     /locus_tag="PLK68_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM90962.1"
                     /translation="MWGYIDEQKVILENILSLQRDTFSTLSKTFISPKEIIITASGTS
                     LNAAMLVKCLIEKEADCSIIVENPFQLRYYSKHLYNPQNILIVISQTGKSTGTLECLQ
                     IAKQHHIPTVSITANGDSPIAKKANIHIDMLCGEENVGPKIKGYSATVLTLHLLLLKI
                     TQNPRYQFITQEYYQSIQELPQNIKASLCWCQKNKHWALAKAMSIVGFGVNYPTAREG
                     TLKILETMQIPVMNFDMEEFMHGPHRTIIKDSYIVLIDTDGCGKILMNNFIDFIKTKT
                     SNYLIISTLKTSNENIIHINQHSYTSSWLNAVIPFQIMCTYLPECNGINSSDPVYSDF
                     ATTVGTRIE"
     gene            2179..>2559
                     /locus_tag="PLK68_10965"
     CDS             2179..>2559
                     /locus_tag="PLK68_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRM90963.1"
                     /translation="MSVVTKRQLNLLQVFMDSNRYLTGQKLSQLLNVSSKTIRNEIKI
                     LNQLMNNFALIQSIPSHGYQISIFDDDLFNCWIDKVSKQWSYFIPTNPLERAYYILGL
                     MLESQEFIKIDDISDILFIDRTSVS"
BASE COUNT          947 a          326 c          453 g          833 t
ORIGIN      
        1 tgctgatttt gtttcaacac aaattagagt tgggggaaat aaggcgcgaa taaaggatga
       61 aaaaataccc ctaaaatatg gcttgattgg acaagaaaca actggagtag gaggaatgtt
      121 taatgcattt agagtaattc cagttatgat tgaaatagct aaagatgttg aaaaatattg
      181 tccagatgct tggattatca attattcaaa tccaacagga ttagtaactg aagcactaaa
      241 taaagtttgt aatgttaaag tggcgggatt atgtgctgga ggaatgcgac caagatggtg
      301 ggctgctgaa gctttaaata ttccagaaga aaaaatctat tatgattatt taggattaaa
      361 tcatatgaat ttttcttata atatgactat tgatggaaga ccgattactg atgaagaatt
      421 tagtaaagtt ataaaaaaat gtacaactgt ttcacaagaa tggatggaaa cattacactt
      481 aattccgagt cagtatacac aatattattt tcatacgaaa gaaagagtac aacaattaaa
      541 agaacaagac aagactagag gtgaagctat tcttgaatta gaaaaacaaa tatttaggga
      601 gtatgcagac gaattaaata atcaaaaacc aaaaacttta gctaaacgtg gcggtggtgg
      661 atattccaaa gttgcgattg gtgttatgga tgcgatttat aatgatagtg gaaaatggat
      721 ggttatcaat gttgctaata atggaacagt tcgtttctta gatgatgatg ctgttattga
      781 aacagcttgt tatgtaagta aaaatggcat gaaaccatta acaattgctc actatcctaa
      841 agctgttatc ggacttatta gtgcagtaaa aacatatgaa tcattagcag ttgaagcggc
      901 gatcacggga aataaaaaac tagcattaga ggctcttgtt gctcatccat tagtaagaga
      961 ttatgatgta gcaaaaccac ttttagagga gatgctggaa gcccataagg aatatcttcc
     1021 acaattttat catcatggaa aataagatgt ggggctatat tgatgagcaa aaagttattt
     1081 tggaaaatat tctttcttta caaagagata cattttctac tctttctaag acatttataa
     1141 gtcctaaaga aattataatt actgcatcag gtacctcatt aaacgctgca atgttagtga
     1201 aatgtttgat tgaaaaagaa gcggattgct cgattatagt ggaaaatcct tttcagttaa
     1261 gatattactc aaaacattta tataaccccc aaaatatact tatagttata tctcaaacag
     1321 ggaaaagtac gggtacatta gaatgtctac aaatagctaa acagcatcat ataccaaccg
     1381 tttcaataac ggctaatggt gatagcccaa tcgcaaaaaa agccaatatt catattgata
     1441 tgttgtgtgg agaagaaaat gttggtccta aaattaaagg atatagtgca acagtactaa
     1501 ctttgcattt gctattgttg aaaataactc aaaatccacg gtatcaattt atcactcaag
     1561 aatattatca aagtatccaa gaattacctc aaaatataaa agcttccctt tgctggtgcc
     1621 aaaaaaataa gcattgggct ttagcaaaag caatgagtat cgttggtttt ggagtgaatt
     1681 atccaactgc tagagaagga acgctaaaga ttttagaaac aatgcaaatt cctgtgatga
     1741 attttgatat ggaagagttt atgcatggtc cgcatagaac gattattaaa gattcttata
     1801 ttgttcttat tgatactgat ggatgtggaa agattttgat gaataatttt attgatttta
     1861 taaaaactaa aacaagcaat tatcttatta tttcaacttt aaaaacaagt aatgaaaata
     1921 ttattcacat taatcagcat tcatatacat cttcatggtt aaatgctgtg ataccttttc
     1981 aaattatgtg tacttattta ccagaatgta atgggattaa ttcatctgat cctgtataca
     2041 gtgattttgc tactacagta gggacacgga ttgaataaaa tagatcagcc attaaggctg
     2101 atttattttg tctatctgtt attttacaaa taaataaaac ttattatata attgaaataa
     2161 tagaaggagg aattaaatat gagtgttgtt actaagagac aattaaatct tttgcaagta
     2221 tttatggatt ctaatagata tttaactgga caaaaactat ctcaattatt gaatgtgagt
     2281 tctaaaacta ttagaaatga aattaaaata cttaatcaat tgatgaataa ctttgcactt
     2341 attcaatcaa ttccttctca tggttatcaa attagtattt ttgatgatga tttgtttaat
     2401 tgttggattg ataaagtatc taagcaatgg agttatttta ttcctacaaa tcctttagaa
     2461 agggcttatt atattttagg tttaatgctc gaaagtcaag agtttattaa aatcgatgat
     2521 atatctgata ttttatttat tgatagaact tctgttagt
//
LOCUS       DAOMTA010000431         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1837_length_2551_cov_1.935897, whole genome shotgun sequence.
ACCESSION   DAOMTA010000431 DAOMTA010000000
VERSION     DAOMTA010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_2551_cov_1.935897"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLK68_10970"
     CDS             <1..474
                     /locus_tag="PLK68_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90964.1"
                     /translation="MTAQQTATILEDEVNVIVIPTKTIPQGLSACIMFNPDASLDDNV
                     AEMTEAVGNVKTGQVTFAIKDTNIDGVEIKANDYMALVEKDIVACKDNKLKALKVVLE
                     KLVDEDAELITLIYGEDVNDDDIEEIESFVEDNFEAELEVVNGKQPVYSFIVGVE"
     gene            complement(599..>940)
                     /locus_tag="PLK68_10975"
     CDS             complement(599..>940)
                     /locus_tag="PLK68_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM90965.1"
                     /translation="QVKDYQDKMKDDLKDVWQNLNLVFTQDTGGYIQKANINNRLNSL
                     KKGTNYEDITVHSLRHSNATLLLLNGVDLKIVSAHLGHNDIQTTANIYIDVLKSQQQM
                     VAQLIEFNLEI"
     assembly_gap    941..950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<951..>1168)
                     /locus_tag="PLK68_10980"
     CDS             complement(<951..>1168)
                     /locus_tag="PLK68_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM90966.1"
                     /translation="SEDFSTYNAVIHFQLYTGMRIGETLALTWDDIDFNQRLIHINKT
                     VNFVKGEFKIGPPKTENSYRVLGMNETV"
     assembly_gap    1169..1178
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1179..1881)
                     /locus_tag="PLK68_10985"
     CDS             complement(<1179..1881)
                     /locus_tag="PLK68_10985"
                     /inference="COORDINATES: protein motif:HMM:NF026010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90967.1"
                     /translation="MAIEERRTKTGISFKIIAYDGYKLDKDGKYIQVKKSTTYRPPKD
                     MPLRQARRIAQQMELDFTDQYKKHQANGINMRLSEVWDWFEKYYAPNYLRESTIETMR
                     SIVEAKILPDIGHIKLGDFSPNRITMFLYDAAIYKDRDGNPIKPTKYYKDSYTQLIFS
                     KLHSLFDAAVKQGWIKDNPCTNAIKPKRNNEYEKHEYPYADPCGPSGGHRAGHESCWP
                     GQCAHGPALHELPDPA"
     gene            complement(1882..2238)
                     /locus_tag="PLK68_10990"
     CDS             complement(1882..2238)
                     /locus_tag="PLK68_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90968.1"
                     /translation="MKIKTTNNRFKVARINAGYSQRDVSCILDFLSFQALSLYEQGKR
                     VPSNMVLYSLPKLYHVSLDYLLNEDDYRNHEEFIISELGLNEKSILILKSNSEHTLIN
                     KVLKEFIQSNKTRSDE"
     gene            complement(2216..>2551)
                     /locus_tag="PLK68_10995"
     CDS             complement(2216..>2551)
                     /locus_tag="PLK68_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM90969.1"
                     /translation="SGLSQPALSSVLGFKGGKATIGYYERNKRLPNVATLIKMKNIFQ
                     VSLDYLLCLDDYPTHIDYTKNELGLDDETIQLLLELSTNKELNMKLNDFIQHQLLKEI
                     ETHENKDNQ"
BASE COUNT          783 a          369 c          445 g          934 t
ORIGIN      
        1 atgactgcgc aacaaacagc aacaatttta gaagatgaag taaatgttat tgttattcca
       61 acgaaaacta ttcctcaagg gttatcagca tgtatcatgt ttaatcctga tgcttcattg
      121 gatgataatg ttgctgaaat gacagaagca gtaggaaatg taaaaacagg acaagtaact
      181 tttgcaatta aagatactaa tatcgatggt gtagaaatta aagctaacga ctatatggct
      241 ttggtggaaa aagatattgt tgcttgtaaa gataataaat taaaagcatt aaaagttgtt
      301 ttagaaaaac tagtagatga ggatgctgaa ttgattacat tgatttatgg tgaagacgta
      361 aatgatgatg acattgaaga aattgaaagt tttgttgaag ataattttga ggctgaatta
      421 gaagtagtaa atggtaaaca gcctgtttat tcatttatag ttggtgtaga ataataaaac
      481 cattgaaaaa gtgccctatt tatcggcact ttttgcatgt tggattgtat caggggctta
      541 ttaggggctt aaaattagtt gatagaaata aaaactaatg aattttaggg aacacatact
      601 agatttcaag attaaactca atcagttgtg cgaccatctg ttgttgagat tttaagacat
      661 caatatagat gttggcagtg gtttgaatat cattgtgccc taaatgtgct gatacaattt
      721 ttaaatcaac accatttaaa agtaataaag ttgcatttga atggcgtaag gaatgaacgg
      781 tgatgtcttc atagtttgtt ccttttttta atgagtttaa gcgattatta atgtttgctt
      841 tttgaatgta accaccagta tcttgagtaa atactaaatt taaattttgc caaacatctt
      901 ttaaatcatc tttcatctta tcttgataat cctttacttg nnnnnnnnnn aacagtttca
      961 ttcattccta acactctgta actattttct gttttaggtg gaccaatttt aaattcacct
     1021 tttacaaaat tcacagtttt attgatatgg ataaggcgtt gattgaaatc tatgtcgtcc
     1081 catgtaagtg caagtgtttc tccaattctc attccggtat atagttgaaa atggataact
     1141 gcattatagg tgctgaaatc ttcacttann nnnnnnnngg gcagggtcag gaagctcatg
     1201 taaagcgggc ccatgggcac actgcccagg ccaacagctt tcatgaccag ctcgatggcc
     1261 accagaaggg ccacacgggt cagcgtacgg gtattcatgt ttttcatatt cattgtttct
     1321 ttttggttta atagcgttgg tacagggatt gtcttttatc cagccttgtt taacagctgc
     1381 atcaaaaagt gaatgtaatt ttgagaatat caattgtgta tatgaatctt tatagtattt
     1441 agtaggtttt ataggattgc catctctatc tttgtatatt gcagcatcat aaagaaacat
     1501 ggtaattcta ttcggtgaga aatcaccaag ttttatatgt ccaatgtcag gaagaatttt
     1561 tgcttctaca atgcttcgca tggtttcgat ggtagattct ctaaggtagt ttggtgcata
     1621 gtatttttca aaccaatccc atacttcact taatctcata ttgattccat tagcttgatg
     1681 ttttttatat tgatctgtaa aatctaattc catttgttgt gctattcttc ttgcttgacg
     1741 taatggcata tcttttggtg gtcggtatgt tgtagatttt ttaacttgta tatatttacc
     1801 atctttatct aatttgtatc catcgtaggc aattatctta aaacttatac ctgtttttgt
     1861 tcttctttct tcaatagcca tctattcatc actccttgtt ttattacttt gtataaattc
     1921 tttgagtact ttgttgatta gtgtatgttc agagttagat ttcaaaatta aaatagattt
     1981 ttcattcaat cctaattcag aaatgataaa ttcttcatgg tttctataat catcttcatt
     2041 aagtaaatag tctaatgaaa catgataaag tttaggtaag gaatataaaa ccatgttact
     2101 aggaactctc tttccttgtt cataaagaga taatgcttga aaacttagaa aatcaagaat
     2161 acatgataca tctctttgag aataaccagc atttatacgt gcgactttaa agcgattatt
     2221 ggttgtcttt attttcatga gtttcgatct cctttagcag ttgatgttga atgaaatcat
     2281 ttaatttcat atttaattct ttgttggtag aaagttctag taacaactgt atagtttcat
     2341 catctaatcc gagttcattt tttgtataat ctatatgtgt tggataatca tctaagcaca
     2401 gtaaataatc taaagatact tggaagatgt ttttcatttt aattaaggtt gctacattcg
     2461 gcagcctttt attgcgttca taatatccaa tagtggcttt gccgccttta aatcctaaaa
     2521 cactactgag tgcgggttga gaaagaccac t
//
LOCUS       DAOMTA010000432         2548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1839_length_2548_cov_2.160850, whole genome shotgun sequence.
ACCESSION   DAOMTA010000432 DAOMTA010000000
VERSION     DAOMTA010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_2548_cov_2.160850"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1945)
                     /locus_tag="PLK68_11000"
     CDS             complement(173..1945)
                     /locus_tag="PLK68_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM90970.1"
                     /translation="MLYLKRILIVGLSFVVGLINIIGNNYTVFALEKNTLVHQNVMSM
                     IQSQRGGLFDPIYTESLEDNWQGDYIYYGEDTDGSALKWQLLDSDNKDFSKNANSTMF
                     LFSDKTIGTSFWYRENDYGFDYSNVKPGETPIYWQKGYEWDKSDLKTNMEDEMKNKFS
                     SIENEGIIFSSKSSEDAQNVAVGLEKLGTTEMTASKWFPLSIQELTNKCYGFSSVPFN
                     RNQAPLGLHTGDKSRVSSDDKNYLIRSYEEHWTKPMENGMRYHPVFVLNSNSVYGANC
                     AGGLNYGNMYTVQSMRPAMNIDTSKVMLVKSHNQKEIIGIKAVNDSKTNEFKLTLLDD
                     KQKLNIENIKTERDKITFDFEAIGNGKYLSVVVQDENNNVMYYGNIKDNLDKVNVGTM
                     TIDLNQIGLNSKSFSSGEYKLLIFTEQLNFDKKTDYSSKFVDVEVVQNNVPPAVINKV
                     PTIVAEDKVLTVGDTFDPLKDVTAYDNEDGIIKLTEANIAANDVNTNKEGTYNVTYKV
                     TDKQGASSTKTITIDVIERIVIPENKPVDKTNNDTNSSNSSKVPQTGDINNIGVLAVT
                     LMMSGSIVIGCNRKKSKANSLSEK"
     gene            2260..>2548
                     /locus_tag="PLK68_11005"
     CDS             2260..>2548
                     /locus_tag="PLK68_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90971.1"
                     /translation="MLNNLSTKKKLMLLPIIFIVIVIVCALVFSYFNQINNQRIKTAT
                     QTDIFIQQVLKGRISVYQFLRVPSEQSAQKVKSDFKELDDFVKNLKPFLTEK"
BASE COUNT          819 a          445 c          272 g         1012 t
ORIGIN      
        1 ggctgtcttt atattatcaa taatctatat ctacccttat aagttttaga acatcataat
       61 agagataaat caattttaat ctaataaata taaatattta tcaatttagc gtatttttaa
      121 atcttgctaa tactagatgg ctaatttaaa aaatattaat ttttttaata ttttattttt
      181 cgcttagact attagcctta cttttctttc tgttacatcc tattacgata cttcctgaca
      241 tcataagtgt gaccgctaag acacctatat tattaatatc acctgtttgt ggcaccttac
      301 tagaattaga agaatttgta tcattatttg tcttatctac tggtttattt tctggtataa
      361 ctattcgttc tattacatca attgtaattg tcttagttga tgaagcacct tgtttatctg
      421 ttactttata agttacatta tatgttcctt ccttatttgt atttacatca tttgctgcaa
      481 tatttgcttc cgttaacttg attattccat cttcattatc atatgctgtc acatctttca
      541 gtggatcaaa tgtatctccc actgtcagta ctttatcttc tgccacaatt gttggtactt
      601 tattaataac agcgggtggt acgttattct gtacaacttc tacgtctaca aatttgcttg
      661 aataatccgt ctttttatcg aaattcaact gttctgtaaa aatcaataat ttatattctc
      721 ctgaactaaa tgatttagaa ttcaatccaa tttgatttaa atcaatcgtc attgttccta
      781 catttacttt gtctagatta tcttttatat tcccataata cataacatta ttattttcat
      841 cttgaactac aacactcaag tattttccgt ttcctattgc ttcaaaatca aaagttatct
      901 tatcacgttc ggtcttaata ttttctatat ttaatttttg tttatcatca agtaatgtta
      961 atttaaactc atttgtctta ctatcgttga ctgctttaat accaataatc tctttttgat
     1021 tatgactttt aaccagcata actttagaag tgtctatgtt cattgctgga cgcatacttt
     1081 gtactgtgta catatttcca taatttaatc ctccagcaca atttgcacca tatacactgt
     1141 tactatttaa cacgaataca ggatgatacc gcattccatt ttccattggc tttgtccaat
     1201 gttcctcata tgaacgtatt aaataatttt tatcatcaga actaacacga cttttatctc
     1261 cagtatgtaa tccaagaggt gcttgatttc tattaaatgg aacactgcta aaaccataac
     1321 acttattggt aagctcttgt atacttaaag gaaaccattt actagcagtc atttctgtag
     1381 tacctaattt ttccaaacca accgctacat tttgtgcatc ttcactactt tttgaagaaa
     1441 aaataatgcc ctcattttca attgacgaaa acttattttt catttcatct tccatattag
     1501 tctttaaatc acttttgtcc cattcatagc ctttttgcca atatatagga gtttcccctg
     1561 gctttacatt actataatca aaaccataat cattttcacg ataccaaaaa ctagttccaa
     1621 tagtcttatc agagaacaaa aacatagtac tatttgcatt cttactaaaa tccttattat
     1681 ctgaatctag gagttgccat ttcaatgctg aaccatccgt atcttctcca tagtagatat
     1741 aatcaccttg ccaattatcc tctaaactct ctgtataaat cgggtcaaat agtcccccac
     1801 gttgagactg tatcattgac ataacattct gatgaaccag cgtattcttt tctaaagcaa
     1861 atactgtata attatttcca ataatattta taagtcctac tacaaaagat aatccaacta
     1921 ttaatattct ttttaaatac aacattcagt ttctccttat tttaaatcaa atctatcaag
     1981 attcataact tttgtccaag ctgcaataaa gtcatttaca aatttttctt ttgaattgta
     2041 taaataaatt tggacataaa actagcatca ttattttttg aattaagatc atttatacta
     2101 tcactaagat ttgtaactgc ccttaaaaaa atgttctcaa ttataatatt tataataaat
     2161 ttttataatg ctatttgttt gcatctatct tttgttacta tgattctatt attatattag
     2221 ttatcaatag taatcttatg aacttttgga gatatcgcca tgcttaataa tctttctaca
     2281 aaaaagaaat taatgctttt acccattata tttatagtaa ttgtaattgt ctgtgctttg
     2341 gtatttagtt attttaatca aataaataat caaagaatta aaaccgcgac acaaacagat
     2401 atttttatac aacaagtatt aaagggaaga atttctgtat accaattttt aagagttcct
     2461 tcagaacaaa gtgctcaaaa agttaaaagt gattttaaag agttagatga ctttgttaaa
     2521 aatttaaaac cttttttaac agaaaaag
//
LOCUS       DAOMTA010000433         2541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1842_length_2541_cov_1.513677, whole genome shotgun sequence.
ACCESSION   DAOMTA010000433 DAOMTA010000000
VERSION     DAOMTA010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_2541_cov_1.513677"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1189)
                     /locus_tag="PLK68_11010"
     CDS             complement(260..1189)
                     /locus_tag="PLK68_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRM90972.1"
                     /translation="MKTKKFKEISNEWIELKKLSVKYPTIAKYRVVIDTQLAHQFNDY
                     TMEQFNEDIIIAYFNKLSTKENYANSTLLSIRYVLRAIINYSHQKYKTNNCSFELIKI
                     AKKQRQINTLSNSQKINLSNYCFNNFQPISIAVAISLYTGLRIGEICALKWEDINFED
                     NYIYVYKTVERLKSKEDTGNKTALMILNPKTSSSKRIVPIPLFLNEFLVNYKSRYQIE
                     DNNVFIITGNNKIPDPRTTQYRFNKLCKQFDFNTNFHTLRHSYATNCVMNEVDTKSLS
                     EMLGHSNVGTTLNLYVHSSLEFKKKQINKISRL"
     gene            complement(1672..>2541)
                     /locus_tag="PLK68_11015"
     CDS             complement(1672..>2541)
                     /locus_tag="PLK68_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRM90973.1"
                     /translation="GAEVNVAIGLTRLGHQATYLTVLGDDPWGHYIKKKLYQEGIDTR
                     LVYYNSLFPTGMMMKNQVIEGDPDIYYYRQGSAFSNIDTNLIDQINLNDFDQLHITGI
                     PLALSSKTRETSLRLVKKAREAGLYISFDPNLRPSLWPNQTTMIDTTNEMAKYCNMFL
                     PGNNEGKILMGSDDPEKIAVYYHQLGCDELVIKLGSQGAYYSSKNETLTVPGFNVKKV
                     IDTVGAGDGFAAGIISGHLEALCSKDMLIRANAIGAIQVQTLGDNEGLPNIEELNAYL
                     KKSHCEANPESWI"
BASE COUNT          828 a          371 c          368 g          974 t
ORIGIN      
        1 aaattaagtt tttttattat tataaattgg tttggctcta cgatttttat cgtagagttt
       61 ttttgtattt gaattaagtg tagaataggg cacttactat taaacgctag atcataaaat
      121 ttatttctta tgcataaaag attctcataa agaagccaca actaatttct tatttccaat
      181 aatttaataa aaacaggctg aaataaagta attaaatttt atttttaacc tgttttaaaa
      241 taaaattatt tataaaaaat tatagtctgg aaattttatt aatttgtttc tttttaaatt
      301 ctaaacttga atgtacataa agattaagcg tggttcctac atttgaatga ccaagcattt
      361 cactaagtga tttagtatcc acttcgttca taacgcaatt agtagcataa gaatggcgaa
      421 gagtatggaa gttagtatta aaatcaaact gtttacataa tttgttaaaa cgatactgag
      481 ttgttctggg atcaggtatt ttattattgc cggtaatgat aaaaacatta ttatcttcga
      541 tttgatatcg acttttataa ttaactaaga attcattaag aaataatgga ataggaacaa
      601 ttcttttaga tgaactggtt ttagggttaa gtatcatgag tgcagtttta ttaccggtgt
      661 cctccttgct ttttaaacgt tcaactgttt tatatacata aatatagttg tcttcaaaat
      721 taatatcttc ccattttaaa gcacaaattt caccaatccg aagaccggta tataaagaaa
      781 tggcaacagc tatagagatt ggttgaaaat tattaaagca gtagttagat aaattgattt
      841 tttgagaatt agacaacgta ttaatttgtc tttgtttctt ggcaatttta attagctcaa
      901 aactacaatt attagtttta tatttttgat gagaataatt aataatggct cttaatacat
      961 atcgaatact tagaagggta ctattggcat agttttcttt agtagacagt ttattaaaat
     1021 aggcaatgat aatatcttca ttaaactgtt ccatcgtata atcattgaat tgatgggcta
     1081 actgtgtgtc aataacgact ctatatttag caattgtcgg atattttact gatagctttt
     1141 ttaactcaat ccattcattt gatatttctt taaatttttt tgtcttcata atttgctttt
     1201 ctccttattt ttatatttac ttttctatga atacgtcaat tatttgacat taaaaaagta
     1261 taaagaaaaa attaatatac agatagtgtc aaaaatcatt acaaaaattt atatttagtt
     1321 ttttattaca aattcttaaa ttatcgaaat actcttgaat atgtatatct tcttttatag
     1381 gatactgagt tggttccttg actgtatcta aagctataaa gcgtacttct ggtggtgtta
     1441 tatttttgaa tcgttcttgt aacttttgtt attgtagaaa tttatatagg aattaatgga
     1501 ttcttttaat ccttcataaa catgaaattt ctttaaatca tgcacattgt gctttcctag
     1561 cttataccct tttgataatg aaataagtaa tattccttgt atttgtttaa tatagatacc
     1621 aattttttgc tctgttgttg gctttgctat aataaaaacc ccaaaagttg gttatatcca
     1681 gctttcgggg tttgcttcac aatgtgactt ttttaaatag gcatttaatt cttcaatatt
     1741 tggtaatcct tcgttatctc caagtgtttg aacttgaatc gcgccaattg cattcgcacg
     1801 aatcaacata tctttactac ataatgcttc caaatgacct gaaataattc ctgctgcaaa
     1861 gccatctcca gcaccaactg tatcaatcac tttttttaca ttaaatccag gaactgttaa
     1921 agtttcattt ttactgctgt aataagcacc ttgggaacca agctttatta cgagttcatc
     1981 acagcctagc tggtgataat atacagcgat tttttcagga tcatcactgc ccatgagaat
     2041 ttttccttcg ttattgcctg gtaagaacat attacaatat ttggccattt cattagtggt
     2101 atcgatcatt gttgtttgat taggccaaag agatggtctt aagttcggat caaatgaaat
     2161 ataaagaccg gcttcacgag ctttttttac aagccttaaa ctagtttctc gagttttaga
     2221 agacagtgct agggggatac ctgtaatatg taattggtca aagtcattta aattgatttg
     2281 atcaattaga ttagtgtcaa tattcgaaaa agcagaacct tgacgataat agtaaatatc
     2341 aggatcaccc tcaataactt gatttttcat catcattcca gttggaaaaa gactattata
     2401 gtaaactaat cttgtatcaa ttccttcttg ataaagcttt tttttaatgt aatgacccca
     2461 tggatcatca cctaaaacag ttaaatacgt agcttgatgc cctagtcttg ttaatccgat
     2521 agcaacatta acctcggctc c
//
LOCUS       DAOMTA010000434         2536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1843_length_2536_cov_2.165659, whole genome shotgun sequence.
ACCESSION   DAOMTA010000434 DAOMTA010000000
VERSION     DAOMTA010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1843_length_2536_cov_2.165659"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..604)
                     /locus_tag="PLK68_11020"
     CDS             complement(65..604)
                     /locus_tag="PLK68_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM90974.1"
                     /translation="MKNLSVRNIVLAGFFLAVGIVLPFFTMQIPSIGNMLLPMHIPVL
                     ICGFVCGWPLGLIVGFILPLLRSMLFTMPPMYPTALAMAFELAAYGALTGLFYNRLAK
                     KPLNTYIALGIAMIGGRVIWGIISAILYGMAGQPFGFAIFIAGAFTNAIPGIILQLIV
                     IPILIMALENAHLIKYNND"
     gene            complement(1290..2048)
                     /locus_tag="PLK68_11025"
     CDS             complement(1290..2048)
                     /locus_tag="PLK68_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRM90975.1"
                     /translation="MSFEMLSDLGIKKYEMCRDDIGRFFARSVVAGLYLGLATILSTT
                     LGTLLFKDNLIASKIAVAGSFGIGLVIIVILGSELFTGNCFTTMIPVYGKKLKFRQII
                     PMWIVCYFGNFVGIALVCYLFFISGSNHELLSEYVVSCANTKLSFDIIELLVKAVLCN
                     FIVCVGAYVGMKMQDDTAKTIIMMIVVMAFVLPGFEHSIANMGSFTLTIGVLGSGANF
                     GLIAIHMIVVTFGNMIGGGVLLGLPLYLMIKPNK"
     gene            2247..>2536
                     /locus_tag="PLK68_11030"
     CDS             2247..>2536
                     /locus_tag="PLK68_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM90976.1"
                     /translation="MFKKGSYIYYALGNGMFYFSWAMFSCIISVYLAGINCSATEISL
                     ITSAAALFAMATQPITGFLADKFKSPKLVAIITGALTIIFGLLFASTKSFIF"
BASE COUNT          932 a          475 c          310 g          819 t
ORIGIN      
        1 caatttaata atatcctgag ccttcatttg gggatatttc tctagctgag ttgtaacgag
       61 tgcactaatc attattatat ttaattaaat gcgcattttc taatgccata attaatattg
      121 gaatcacgat cagctgtaat atgattccag gaattgcatt ggtaaaagct ccagctataa
      181 agattgcaaa tccaaatggc tgtcctgcca ttccatataa tattgcacta ataattcccc
      241 agattactct tccaccaatc atcgctattc ctaaagcaat ataagtattg agcggctttt
      301 tagctaaacg attataaaat aaccctgtta aagctccata agcagctaat tcaaaagcca
      361 tagctaatgc agttggatac attggcggca ttgtaaataa catacttcta agcaatggta
      421 agataaagcc aacaattaat cctaacggcc acccgcaaac aaacccacaa atcaatactg
      481 ggatatgcat tggtaacaac atgttgccga tacttggtat ctgcatcgta aaaaacggta
      541 aaacaattcc tactgccaag aaaaaaccag ctaaaacgat atttctaaca cttaaatttt
      601 tcatttttcc ctccttataa aacatgaaaa aagccataaa aatacctgtt tttcaaaaca
      661 gtctactacc ttcatggcat aatgttatct tcttgacaac gtctattctt gctttaatca
      721 tgtataaaaa ttttatcgat aaaccacagt tttgtcaaca tcaataatca agttcaatcg
      781 cttaggcaat aatttttttt aaaataaaag agttattgat gactgtcagt agaaattatt
      841 agcgaagtaa aaattgtgta tgtatgcacc acaaaataat cttaaattaa ctattttcct
      901 atattcaggt aaaataaagg ttaccaaaaa tttaatcctt taattggact agcttcatca
      961 aaataattaa tttcctatca atatcaagct agtattattg aagaagatga gcgttttatc
     1021 tctataggca cattttaaaa gagcaattaa ctatactgat acttaaaaat aatcatcaaa
     1081 acatacaatt tctaccaatc aattaacacc tctatgttat cgtactttta tcaacaaaga
     1141 tgattatgat gttattctta gtataaagct gattgattag gtatttttga tattgatata
     1201 atttattccc gaacaaaaat ttaaaaccgt aataaatgca aataaaaaaa tctattaata
     1261 atgaatcata aattcattat catagatctt tatttatttg gcttaatcat tagatatagt
     1321 ggtaatccta acaaaactcc accaccaatc atattaccaa aagttacaac gatcatatgt
     1381 atagcaatca aaccaaagtt agcaccactt cctaaaaccc ctatcgttaa agtgaaagaa
     1441 cccatattag caatactatg ttcgaatcca ggtaaaacaa atgccataac cacaatcatc
     1501 ataataattg ttttggccgt atcgtcctgc atcttcatcc ctacgtaagc acctacacaa
     1561 acgataaagt tacataaaac tgctttgact aataattcaa tgatatcaaa acttaatttt
     1621 gtatttgcac aactaacaac atattccgaa agcagttcat gattactacc actaataaaa
     1681 aataaataac aaactagtgc aattcctaca aaattcccaa aataacaaac tatccacatg
     1741 ggaattatct gacgaaactt caatttctta ccataaacag gaatcattgt tgtaaaacaa
     1801 tttcctgtaa ataactctga tcctaaaatt acaataatta ctaatccgat tccaaatgaa
     1861 cctgctacag caatttttga agcaattaaa ttatctttaa atagcaatgt tcctaatgtt
     1921 gttgataaga tcgtcgctaa tcctaaatat aatcccgcaa ctactgaacg agcaaagaag
     1981 cgcccaatat catcacgaca catttcatat ttttttatcc ctaaatctga taacatttca
     2041 aaactcatat tgaaaccctc caaacctgtt tcattataaa ccattattct tccaagttca
     2101 acaaattact tgtctggtgc tatgttgtaa aaaatatagc tcttataccg gattctagat
     2161 attcttttcc aaaaaatgaa aaagcgctta cttaaaaaat ttcagtgata aaatcgaagc
     2221 atcaaaaaat atttggaggg tcatacatgt tcaaaaaggg aagttatatc tactacgcct
     2281 taggaaatgg gatgttctat ttttcatggg caatgttttc ttgtatcatt tctgtctatc
     2341 ttgctggaat aaactgctcg gctactgaaa tttcattaat cacttcggct gcggctttgt
     2401 ttgcaatggc gactcaacca attacaggtt ttctagctga taaatttaaa agtccaaaac
     2461 tagtcgcaat tataactggt gcattaacaa ttatttttgg tttactattt gcttcaacaa
     2521 agtcttttat cttttt
//
LOCUS       DAOMTA010000435         2533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1845_length_2533_cov_3.000807, whole genome shotgun sequence.
ACCESSION   DAOMTA010000435 DAOMTA010000000
VERSION     DAOMTA010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1845_length_2533_cov_3.000807"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLK68_11035"
     CDS             complement(<1..627)
                     /locus_tag="PLK68_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRM90977.1"
                     /translation="MFKRILIANRGEIAVRIIRTCQELGIEAVAVYSNVDSTSLHVQL
                     ADHAVCIGPAKAADSYLNMKNILSVATALGCDAIHPGFGFLSENSTFARLVEECGITF
                     IGPSGDVIDMMGNKSMARQKMIEAGVPVVPGSDGSVNTLQEAKEVANQIGYPVLIKAS
                     AGGGGRGMRKAFSEEEFDDAYLTAKAEAKACFGDDDMYLDVLESLYLLD"
     gene            complement(633..1076)
                     /gene="accB"
                     /locus_tag="PLK68_11040"
     CDS             complement(633..1076)
                     /gene="accB"
                     /locus_tag="PLK68_11040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRM90978.1"
                     /translation="MKIDLVKQLINEFENSEVFKMKVEIDDIKLELEKTPVAPVNVVS
                     TPVVSAPTPTLAPVEQVAANEPAITGTPVKSPIVGVYYASSSPTAKPYVEVGTKVKAG
                     QVLCIVEAMKVMNEIKAPIDGTVTSIMANTEDLVEFDQVLMIIEG"
     gene            complement(1163..1390)
                     /gene="acpP"
                     /locus_tag="PLK68_11045"
     CDS             complement(1163..1390)
                     /gene="acpP"
                     /locus_tag="PLK68_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM90979.1"
                     /translation="MEFEKVKEIIVDSLSCDEDAVTLEANLKEDLDADSLDAVELIMA
                     VEEEFDIEIPDDKAAEIKTVQDIVDYIKENA"
     gene            1602..2495
                     /locus_tag="PLK68_11050"
     CDS             1602..2495
                     /locus_tag="PLK68_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM90980.1"
                     /translation="MKIPEIKAKTIIQNKADKNHFWFGFDYNMNLYQGCHHGCIYCDS
                     RSDCYQISNFDQIKVKHNVLTLLSKELCSKKRKGVIAIGAMSDPYNHYEQQLKITHQA
                     LKIIKQTNFGVMITTKSHTLINDLELIKQINDKQSVLICMTITCGDDSLAKLIEPNVS
                     ISSKRFETIKQLRQHNIYAGVLMTPILPFINDTPENIREIIYRAHLANASFVYPMFGV
                     TLRDHQRDYFYTQLDHLFPGCKERYIKQFGNTYLCNSPRLYELKNIFIEECHKYNIIY
                     KMEDIINGYKQTASSEQLSLF"
BASE COUNT          833 a          492 c          334 g          874 t
ORIGIN      
        1 gtctaataaa tataaactct ctaatacatc taaatacata tcatcatcac caaaacatgc
       61 tttagcttct gctttagcag ttaaataagc atcatcaaat tcttcttcgc taaaggcttt
      121 tcgcattcca cgaccaccac cacctgctga ggctttaatt aaaactggat aaccaatttg
      181 atttgcaact tctttagctt cttgtaaagt atttacactt ccatcactcc caggaactac
      241 cggcacacct gcttcaatca ttttttgacg ggccatactt ttatttccca tcatatcaat
      301 aacatcgcca ctaggcccaa taaaagttat tccacattct tctactaaac gagcaaaagt
      361 tgagttttct gataaaaagc caaatcctgg atgaattgca tcacaaccta gtgcagtcgc
      421 tacacttaaa atatttttca tatttaaata actatccgct gccttggcag gacctataca
      481 taccgcatga tcagccaatt gcacatgcaa tgaagtacta tcaacatttg aataaacagc
      541 aactgcttca attcctaatt cttggcatgt tcgaattatt cttaccgcga tttcgccacg
      601 gttggcaatt aaaattcgct taaacatctt aactaccctt caatgatcat taacacttga
      661 tcaaattcaa ctaaatcttc agtatttgcc atgattgatg ttactgttcc atcaattgga
      721 gctttaattt cattcattac cttcattgct tcaacaatac acaacacctg tcctgctttt
      781 actttagtgc ctacttctac atacggttta gcagttggtg aactagatgc ataataaacc
      841 ccaacaattg gtgatttaac cggtgttccg gtgattgctg gttcattagc tgctacttgc
      901 tccactggcg ctaaagtagg cgttggagca gaaactaccg gtgtacttac aacatttacc
      961 ggtgctactg gcgttttttc taattctaat ttaatatcat ctatctctac tttcatttta
     1021 aaaacttctg agttttcaaa ttcatttata agttgcttta ctaaatctat tttcataaat
     1081 actctccttt attttgaata tcaaagtaat tttttaaaaa aattattacc cagctattct
     1141 gggtaataaa aatcagcatt tattatgcat tttctttgat ataatctacg atatcttgaa
     1201 ctgttttaat ttcagctgct ttatcatcag ggatttcaat atcaaattct tcttcaactg
     1261 ccatgattaa ttcaactgca tctaatgaat ctgcatctaa gtcttctttt aagttagctt
     1321 ctaaagttac tgcatcttca tcacagctta aagaatcaac aattatttct tttacttttt
     1381 caaattccac gttatattcc tcctcgtgta tattacggtc acattataac aaaatcaaaa
     1441 catttgtaaa tagtttgatg ttcaaaactt ttatcgaaga ctgtcgaatt aacccaaaac
     1501 ctagtaaaat caaggtgttt caagtaatat tttttttcgt tttttttatg ttttaatcgt
     1561 cattttttta tgttatagtc ttttttagga gggcacaatt aatgaaaata cccgaaataa
     1621 aagctaagac aatcattcaa aacaaagccg ataaaaatca tttttggttt ggttttgact
     1681 acaacatgaa tctctaccaa ggatgtcatc acggttgtat ttattgtgac agtcgcagtg
     1741 attgttatca aatctcaaac tttgatcaaa ttaaagtaaa acataacgta ctcaccctac
     1801 tttctaaaga actttgttct aaaaaaagaa aaggggtgat tgcaattgga gccatgtctg
     1861 atccctataa tcattatgaa caacaattaa agataactca tcaagcttta aaaataataa
     1921 agcaaaccaa ttttggtgta atgataacga ctaaaagtca tacgctcatt aacgatcttg
     1981 aactcataaa acaaattaat gataaacaat ctgtcctaat ttgtatgacc attacttgtg
     2041 gtgatgattc attagcaaaa ttgatcgaac caaatgtttc aatttcatca aaacgatttg
     2101 aaacaattaa acaattacgc caacacaata tttatgctgg ggttttaatg acaccgattt
     2161 tgccatttat taacgatacc cctgaaaata ttcgagaaat catctatcgc gcacatttag
     2221 ccaatgccag ctttgtttat cctatgtttg gtgtgacttt acgagatcat caacgtgatt
     2281 atttctatac gcagctagat catttatttc ccggctgtaa ggaacgctat ataaaacaat
     2341 tcggtaatac ttatttatgc aacagcccac gactttatga attaaaaaat atatttattg
     2401 aagaatgtca caaatataat atcatttata agatggaaga tattataaat ggttataaac
     2461 aaactgcatc atcggaacaa ttgagtttat tttaaaaaat agtccagaag atcttctgga
     2521 ctatttagca tgt
//
LOCUS       DAOMTA010000436         2528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1853_length_2528_cov_2.275374, whole genome shotgun sequence.
ACCESSION   DAOMTA010000436 DAOMTA010000000
VERSION     DAOMTA010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_2528_cov_2.275374"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1055
                     /locus_tag="PLK68_11055"
     CDS             57..1055
                     /locus_tag="PLK68_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM90981.1"
                     /translation="MKDKKLTMSDIAKMAGVGKSTVSRYFNGGYIKEETRLKLKKVID
                     ENNYEPSTLAQSLKAKYTKVIGIVVPCLDSITTSRVLMTMDQYLKDHGYTTLIINTNH
                     DEMRELTSIEQLWRMNVDGIILMATAVTMAHQNIAAKLDIPLLFVAQRYGAGVSIIND
                     DYSAGYEVGKYAAYMGHRKICYIGVSGKDEAVGIYRKDGVINGLRDNGVSSIDLLETD
                     FSLEKAHLIALDYLKKKQPTLFIGATDNIALGCLKAINELKLKMPDDISLIGFGGYET
                     SQFINPSLSTVRFNNEETGIKAGQTIIDLIEGNVVDNLQLIGYTLIKGQSVKDLNK"
     gene            1199..>2528
                     /locus_tag="PLK68_11060"
     CDS             1199..>2528
                     /locus_tag="PLK68_11060"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HRM90982.1"
                     /translation="MDYNKIAKEIINCVGGKENITGAMHCATRLRLNLVDESKVDEES
                     LTDIDVVKGTFLAKGQYQIILGPGLVNLVCDEVTNILGIKTDSTLQVEKVEEKGNLLQ
                     RAVQLLSDIFVPIIPAIVAGGLLMGINNILTAQGLFFANKSLIEAYPQWADLASMINL
                     FSNAAFTFLPVLIGFSATKKFGGNPYLGAALGMIMVHPDLLNAYSYGKAGVEVPVWNI
                     FGLSIEAVGYQGTVLPVLGVSWILANIEKRLHKVTPTWLDNLTTPLLATLITGFITFI
                     LVGPLLREAGVLLSDGISWMYNTLGLFGGAIFGFFYAPICLTGMHHSFIAVETQLLAS
                     IKTTGGSFIFPTASMSNVAQGAAVIAILLVTKDKKLKSICSASGISALLGITEPAMFG
                     VTLKLKYPFIAAMVGSAVGSAYLAATKTLANALGAAGIPGFLSIDPKNYLN"
BASE COUNT          857 a          321 c          514 g          836 t
ORIGIN      
        1 ttatattaat aagacgggaa ctgcccgtca ttttctttag gttagaggtt ataattatga
       61 aagataaaaa attaacaatg agtgatattg caaaaatggc tggtgttggc aagagtacag
      121 tatcacggta ttttaatggt ggttatatca aagaagaaac gcgtttaaaa ttaaaaaaag
      181 taattgatga aaataattat gaaccgagca cattagcgca atcattgaaa gcaaaatata
      241 ctaaggtaat tggtattgta gtaccttgtc ttgattcaat tacaacatct cgtgttttga
      301 tgacgatgga tcagtattta aaagatcatg gttatacaac tttaataata aataccaatc
      361 atgatgaaat gcgagaatta acttcgattg aacagttatg gcgaatgaat gttgatggaa
      421 ttattttaat ggcaacggca gtaacgatgg cacatcaaaa tattgctgca aaattagata
      481 ttccactatt atttgtggca cagcgttatg gagcaggagt ctctatcatt aatgatgatt
      541 atagtgctgg atatgaagtt ggaaaatatg cagcatatat ggggcatcgt aagatttgtt
      601 atatcggtgt aagtggtaag gatgaggctg taggaattta tcgtaaagat ggtgtgatca
      661 atggtttaag ggacaacgga gtaagcagca ttgatttatt agaaactgat ttttcattag
      721 aaaaggctca tcttattgct cttgattatc taaaaaagaa acagccaact ctatttatcg
      781 gagcaactga taatatcgct ttggggtgtt taaaagcaat taatgagtta aaattaaaga
      841 tgcctgatga catttcttta attggttttg ggggttatga gacttctcaa tttattaatc
      901 ccagtctttc gacagttaga tttaataatg aagaaactgg aattaaagca ggtcaaacaa
      961 tcattgattt aattgaaggg aatgttgttg ataatttaca gttaattgga tataccttaa
     1021 ttaagggaca aagtgtcaaa gatttaaata aataaaagtg atgatgtttt aataaaaaac
     1081 atcttttttt attaaaaatg tggtatcggt ttcagttttt tgttaaaaag tattgacagg
     1141 gtcacaacaa tcgcttatag tatatttgtg gaaccgatac catagaggag gataaaacat
     1201 ggattataac aaaattgcca aagaaataat taattgtgtt ggtggtaaag aaaacattac
     1261 gggggcaatg cattgtgcaa ctaggctacg tttaaatcta gttgatgaaa gcaaggttga
     1321 tgaagaatca ttaactgata ttgatgttgt gaagggaact tttttagcta aagggcaata
     1381 tcaaattatt ttaggaccag gtctagttaa tttggtatgt gacgaagtaa caaatatttt
     1441 aggaattaaa actgatagta cgctccaagt agaaaaagta gaagaaaaag gaaacttatt
     1501 acaacgtgca gttcagttat taagtgatat ttttgttcca attattcctg ccatcgttgc
     1561 tggcggattg ttaatgggaa ttaataatat tttaacagct caaggactat tttttgctaa
     1621 taaaagtttg attgaagcat atccgcaatg ggctgattta gcatcaatga ttaatttatt
     1681 ttcaaacgct gcatttactt ttttaccagt gttaattgga tttagtgcta ctaaaaagtt
     1741 tggtgggaat ccatatttag gtgcagcttt aggaatgatc atggttcatc cggatctact
     1801 aaatgcttat tcgtatggta aagcaggagt agaagtacca gtatggaata tttttggact
     1861 atcaattgaa gcagtagggt atcaaggaac ggttttacct gtattagggg tatcttggat
     1921 cttagctaac attgaaaaac gtttacacaa agtgacacca acttggctgg acaatcttac
     1981 aacaccatta ttagcaacgt taattactgg ttttattact tttattttag ttggcccatt
     2041 attaagagaa gccggggtct tattatctga tggaatatca tggatgtata atacgctagg
     2101 attattcgga ggtgcaatct ttggattttt ctatgcacca atttgtttaa caggaatgca
     2161 tcatagtttt attgcagttg aaacacaatt attggcatcg atcaagacaa caggtggatc
     2221 attcattttt ccaactgcat caatgtcaaa tgttgctcaa ggggcagcag ttattgcaat
     2281 tttattagta actaaagaca aaaaattaaa aagtatttgt tcagcttcag gtatttcagc
     2341 gttattagga attactgagc ctgcaatgtt tggagtaact ttaaaactta aatatccatt
     2401 tattgcagct atggtaggtt cagcagttgg aagtgcttat ttagcggcaa caaaaacact
     2461 tgctaatgct ttaggggcag caggtatacc aggtttctta tcaatcgatc ctaagaatta
     2521 tttgaatt
//
LOCUS       DAOMTA010000437         2527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1855_length_2527_cov_2.336570, whole genome shotgun sequence.
ACCESSION   DAOMTA010000437 DAOMTA010000000
VERSION     DAOMTA010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1855_length_2527_cov_2.336570"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLK68_11065"
     CDS             <1..328
                     /locus_tag="PLK68_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HRM90983.1"
                     /translation="DNGVLISKSILDFFTAAIFACNLGYVVSVIAIPQFVIFFILFLS
                     AKFIFPLTTPAMINDFKACGGFLMIATGFRMIKVKEFPIADMIPAMVIVMPISWLWVN
                     YIMTVI"
     gene            464..1405
                     /locus_tag="PLK68_11070"
     CDS             464..1405
                     /locus_tag="PLK68_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM90984.1"
                     /translation="MNFGIIGFGNIARKFVKSIEYTDEGKIYAIASHSLTSDDEYVKA
                     HPEVKIYQDYDELLQDRELDAVYIALPHKYHKEWILKALDNHIAVLSEKPMVLTVADI
                     EEIKTKVYTEKGYCLEALKTKFNIGLEHLKQDLSLIGKIKQIEANFCFDATAHQTTSY
                     LFNVDQGGALNDVGSYLLGFVLALVDSDIKNVNSQIDIVAGVEWYFKAKINFNNECTA
                     IVEGAIDRNKERLAIIEGEFGKIEIPMFNRIIDYKIIKNDGTVVERNYPIIGDDMTME
                     IQCFIDDVRAHKTESALHSLEDTKRILELTEIIREAN"
     gene            1406..2386
                     /locus_tag="PLK68_11075"
     CDS             1406..2386
                     /locus_tag="PLK68_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM90985.1"
                     /translation="MKLGILGTGMIVKDLLTTINKLKIESISILGTEQTKKETTILAK
                     QYHLDHCYYDYDEMLRSEIDTVYVALPNHLHFSFAKKALLKGKHVIMEKPITSNVKEL
                     EELKEIAQENDLIILEAVNIHYLPAFKKLKEDIKRIGNIKIMNFNYSQYSSRYDAFKQ
                     GTILPAFDYHKSGGALMDINVYNVNAIISLFGQPVSIGYMANIENQIDTSGILMYDYG
                     SFKGISIGAKDCKAPIISTIQGDKGVIKIDKPVNQMTGYQIIFNDGTEEIYRVDDCEH
                     RLYYEFIEFIRIIDNNDYDFAKKMLELSLIVAKVMNEARNQEKIVFTNDN"
BASE COUNT          925 a          263 c          474 g          865 t
ORIGIN      
        1 tgataatgga gttttgattt ctaaatcaat tcttgatttt tttactgctg cgatttttgc
       61 ttgtaattta gggtatgtgg tatctgtaat tgccattccg cagtttgtaa tcttttttat
      121 tttattttta tcagcaaagt ttatttttcc attgactaca ccagcaatga tcaatgattt
      181 taaagcttgt ggtggctttt taatgattgc tacaggattt agaatgatca aggttaaaga
      241 atttccaatt gcggatatga ttcctgcaat ggttattgtt atgccaatta gctggttatg
      301 ggtcaattat attatgacgg ttatataaaa gtattgtgat tgtactaaaa gtacaaaaat
      361 cacagtattt tttttatttt cttagtactt tgtttccaga atattataaa tatatttaaa
      421 taataaatag ttatgttata acaaggaaaa ggaggcaaaa tcaatgaatt ttggaattat
      481 tggatttggg aatattgcaa gaaaatttgt taaaagtatt gaatacactg acgaaggaaa
      541 gatttatgca attgcatctc attccttaac aagtgatgat gaatatgtaa aggctcaccc
      601 agaagttaaa atatatcaag attatgacga attattacag gatcgagaac ttgatgctgt
      661 atacattgcc ttacctcata aatatcataa ggaatggatc ttaaaagcac ttgataatca
      721 tattgcggta ttatcagaaa aaccaatggt attgacagtt gcagatattg aggagattaa
      781 aacaaaagta tatacggaaa aaggatattg cttagaagca ttaaagacta aatttaatat
      841 tggtttagaa catttaaaac aggatttatc attgattggt aaaatcaaac aaattgaagc
      901 taatttttgt tttgatgcta ctgctcacca aactacctca tatttattta acgttgatca
      961 aggtggggca ctgaatgacg taggaagtta tttgcttggt tttgtattag ctttggtgga
     1021 ttcagatata aagaatgtta attcacagat tgatattgtt gctggagttg aatggtattt
     1081 taaagcaaaa attaatttta ataatgaatg tactgcgatt gtggaagggg caattgatcg
     1141 taataaagag cgtttagcaa ttatcgaagg tgaatttgga aaaatagaga ttccaatgtt
     1201 taatcgtatt attgattata aaatcataaa aaatgatggt actgtggttg aacgcaatta
     1261 tccaattatt ggagatgata tgactatgga gattcagtgc tttattgatg atgtacgagc
     1321 tcataaaact gagagtgcac ttcatagttt ggaagataca aaaaggatat tggaattaac
     1381 agaaataatt agggaggcaa attaaatgaa attaggtatt ttaggaacag ggatgattgt
     1441 taaagactta ttaacaacta taaataagtt aaagattgaa tcaatttcga ttttagggac
     1501 tgaacaaact aagaaagaga ctactattct cgctaagcaa tatcatctag atcattgtta
     1561 ttatgactat gatgaaatgt tgcgaagtga aatcgataca gtgtatgttg ccttgccaaa
     1621 tcatttacat tttagttttg ctaaaaaagc tttattaaaa ggtaagcatg taataatgga
     1681 aaaacctatt acttcaaatg ttaaggaatt ggaagaatta aaagaaattg ctcaagaaaa
     1741 cgatctaatt attttagaag cagttaatat ccattattta cctgctttta aaaaattgaa
     1801 agaagatata aagagaattg gaaatattaa aatcatgaat tttaattata gtcaatattc
     1861 ttcacgatat gatgctttta aacaaggaac aattttaccg gcatttgatt atcataaatc
     1921 aggtggagct ttgatggata tcaatgttta taatgttaat gcaataataa gtttatttgg
     1981 tcaaccggta agtattgggt atatggcaaa tattgagaat caaattgata caagtggtat
     2041 tctaatgtac gattatggtt cgtttaaggg gataagtata ggggctaaag attgcaaagc
     2101 accgattatt tcaacgattc aaggtgataa aggggttatt aaaattgata aacctgttaa
     2161 tcagatgacc ggatatcaaa ttatttttaa cgatggcact gaagaaattt atcgagttga
     2221 tgattgtgaa catcgcttat attatgaatt tattgaattt attagaatca ttgataataa
     2281 tgattatgat tttgctaaaa agatgctgga attaagttta atagtagcta aagtcatgaa
     2341 tgaggcaaga aatcaagaaa aaatagtatt tacaaatgat aattaaagga gaagacaaat
     2401 ggaatttaaa agcttgatgc atgtatcttt tttcactgat caaatggatg tgatgagaga
     2461 cttttatgaa aataagttag gattaaaagt taaaattgta acaagggcgg gactttataa
     2521 aggacta
//
LOCUS       DAOMTA010000438         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1857_length_2526_cov_2.623229, whole genome shotgun sequence.
ACCESSION   DAOMTA010000438 DAOMTA010000000
VERSION     DAOMTA010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_2526_cov_2.623229"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLK68_11080"
     CDS             complement(<1..856)
                     /locus_tag="PLK68_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM90986.1"
                     /translation="MKIKSLRHQLVSTTSIIIMTVIVVFSLILLLYMGYYLLIIGRIY
                     AMDEDLITPIASIVAVIFLILIIVALITSIACGFMISKRFLQTVDQFTKSIKQIKNEG
                     LSHRLVIEGNDELALLGKEFNETIDQVERSLLQQNQFVSDASHELKTPLAIIKGNLDM
                     LERWGKDDPAILSNSLNVTSNEVERLIQLCNELLHLTREMDIHCEEPTDLNLVVDEVI
                     TNFKEVHPEFEFIIKITVTSMIWMRIEHLKQLLIILIDNAIKYSREEEKKIELLYVDQ
                     KLMVKDHGI"
     gene            complement(853..1506)
                     /locus_tag="PLK68_11085"
     CDS             complement(853..1506)
                     /locus_tag="PLK68_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM90987.1"
                     /translation="MAILIIEDEKNMSDLLKLELTHAGYCCDQAYDGEVGLKKALEQE
                     YELILLDLMLPRINGIEVCRRLREIKQTPVIMLTARDEVMDKVNGLQVGADDYLAKPF
                     AMEELLARINALLRRMNFQKALLEYSYGEVKIDPSKHKVFFKDNEVNLTTTEFDLLSL
                     LVQNGGDVVSRNKILDQVWGYDEDVSTNVVEVYIRYLRNKIPGIKIETVRGVGYRLS"
     gene            complement(1512..1814)
                     /locus_tag="PLK68_11090"
     CDS             complement(1512..1814)
                     /locus_tag="PLK68_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRM90988.1"
                     /translation="MEKLIIYKKQIITTTIIIVISVICFGVYILHTTLNNINYSKSEA
                     HVVALKQFPGTIVSSQIEYEHMQIFYHLEIENQQQELIEINISAKSGKIIGFEYKE"
     gene            complement(1937..2239)
                     /locus_tag="PLK68_11095"
     CDS             complement(1937..2239)
                     /locus_tag="PLK68_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90989.1"
                     /translation="MLARLYVIIESEDIKDYQMIKDLLLQINPNFSISPSREYAGKKD
                     CSEFFVTANLDVTEVPLLLERLNNDWDGEIDDCSCYGFNTRMFHDLVYYLEFALFE"
BASE COUNT          858 a          454 c          284 g          930 t
ORIGIN      
        1 caatcccgtg atctttaacc attaattttt ggtcaacata taataattca attttctttt
       61 cttcttctct tgaatattta attgcattat caattaaaat aattaaaagt tgttttaaat
      121 gttctatgcg catccatatc attgaagtta cagttatttt aataataaat tcaaattctg
      181 ggtgaacttc tttaaaattt gtaattactt catcaactac taaattaaga tcagttggtt
      241 cttcacaatg aatatccatc tctcgggtta aatgaagaag ttcattacac aattggatta
      301 atcgctcaac ttcattacta gtgacattaa gtgaattgct aagaatagca ggatcatctt
      361 ttccccaccg ctccaacata tctaaatttc cttttataat tgccaatggc gtttttaatt
      421 catgtgatgc atcacttaca aattgatttt gttgtaataa tgatctttcc acttgatcga
      481 ttgtttcatt aaattccttg cctaataaag ctaattcatc atttccctca ataacaagcc
      541 ggtgacttaa gccctcattt ttaatttgtt taatactctt tgtaaattga tcaacagttt
      601 gtaaaaaacg tttactaatc atgaaaccac aggcaatact ggtaatcaat gcaacaataa
      661 ttaaaatcaa aaagattaca gcaacaatac tagctatggg ggttatcaaa tcttcatcca
      721 ttgcatagat tcgtccaata atcaataaat aataacccat ataaagtaat aaaatcaagc
      781 taaatactac gatcactgtc ataataataa ttgaagtagt actaactaat tgatgtcgta
      841 gtgatttaat tttcatgata aacggtatcc tactccacgt actgtttcaa ttttaattcc
      901 gggaatttta ttacgtaaat aacggatata tacttcaaca acatttgttg atacatcttc
      961 atcataaccc caaacctgat ctaaaatctt attgcgacta acaacatcac cgccattttg
     1021 aactaataat gataataaat caaactcagt cgttgttaaa tttacttcat tatctttgaa
     1081 aaagaccttg tgcttacttg gatcaatttt aacttctcca taactatatt caagaagtgc
     1141 tttttgaaag ttcattcttc tcaatagtgc atttatcctc gctaataatt cttccattgc
     1201 aaacggcttt gctaaataat catctgctcc aacttgtaaa ccattgacct tatccatcac
     1261 ctcatcacga gctgtaagca taattactgg cgtttgttta atctctcgca agcgtcgaca
     1321 tacttcgatt ccattaattc gcggtaacat taaatctaat aagattaatt catattcttg
     1381 ctctaaagct ttttttaacc caacttcacc atcataagct tgatcacaac aataacctgc
     1441 atgggttaat tctagcttta atagatctga catatttttt tcatcttcaa ttatcagtat
     1501 tgccatcttc atcactcctt atattcaaat ccaattatct ttccactttt tgcactaata
     1561 tttatctcaa tcagttcttg ctgctgattc tctatttcaa gatggtaaaa tatttgcata
     1621 tgctcatatt caatttgtga agatacgata gttcctggaa attgtttcaa tgctacaaca
     1681 tgtgcttcac ttttagaata attaatgtta tttaaagttg tatgtagtat atagacacca
     1741 aaacaaataa cactaatgac gattattatt gtagttgtta taatttgttt tttataaata
     1801 atcagctttt ccattcccat cttcctttca tctatatcat aacctatttt gcccgattgt
     1861 tgcttaaaga acaataataa ttaaataaga attttctcat tttctaaaaa taaataagtg
     1921 tgatctaaaa tcacacttac tcaaataaag caaattctaa ataataaact agatcatgaa
     1981 acattctagt attaaagcca taacagctgc aatcatctat ttctccatcc caatcattat
     2041 ttaatcgttc taataacagc ggtacctctg taacatctaa attagctgtt acaaaaaact
     2101 cactacaatc tttttttcca gcatactcac gactcggtga aattgaaaaa ttaggattta
     2161 tttgtaacaa caaatcttta atcatttgat aatcttttat atcttcactt tcaataatta
     2221 catataatct cgctaacata taaccacctc tttcctgcct attttatcat aaaatattgt
     2281 ttagataaaa gtatgctatt aattaatttt attctgatta tatttactaa taaatatgtt
     2341 aagataaagt aaagagatat atttgaaagc tgggataatt atggatgaat cactaattac
     2401 gatcattggg tttattattg gaggcctatc tatctttatt tatggaatca atcttatgag
     2461 cgatggcctc aaaagtattg caggatatca tatccgtgag tatattgaaa aatatacaag
     2521 taatct
//
LOCUS       DAOMTA010000439         2526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1858_length_2526_cov_2.552813, whole genome shotgun sequence.
ACCESSION   DAOMTA010000439 DAOMTA010000000
VERSION     DAOMTA010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1858_length_2526_cov_2.552813"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLK68_11100"
     CDS             <1..1097
                     /locus_tag="PLK68_11100"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM90990.1"
                     /translation="NEKGFVFYEHFIDALLKRGIQPIPTLYHFEMPAFLYEKYNGFYS
                     RKVVDIFVELCKKIVDRYHDKVENWIIFNEQNGILQKGPKMFFGAVCPDGVDTQTFDN
                     QIMHNTLIAHSLINEYIHQKGGKVMGMATVVQSYPETCHPLDTLESMKAQSEAYVFLD
                     VFARGHYNSYYFANMKNEGTMPEILDGDLEILKKGITDSLSISYYMSTISHYGEESLT
                     NINDVVIKKNPYLEMSEFGWTIDPTGLRITLRQLYDRYEMPIYIVENGFGYNDQINAD
                     GQIIDDYRIDYMRKHLEEMKLAIQEGVDCRGYLSWGPVDILSSRAQMKKRYGFIYVNR
                     ENDDLKDMRRIRKKSFAWYQQVIASNGEVL"
     gene            1140..2162
                     /locus_tag="PLK68_11105"
     CDS             1140..2162
                     /locus_tag="PLK68_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM90991.1"
                     /translation="MKVTMKDIANKLGISINAVSIALNDKPGVSDEMRLEILKMADKM
                     GYINQKRQYLSVYSKSNICILMQSYYADTGHFYSIVLRSIVEQAKVFGYFSILNYFED
                     GHFVVPECIEERKVAGILVVGKISDSNLLLLKNIGVPVVLVDFTSLCTPCDCVLTHNK
                     QGSYMMTTHVINKGYQRIGFFGDLDYSFSFEDRFIGFKQSLLKNNIVSYSKVDEYIQK
                     YSFLQGIEEYVLTNQIDKIIEILNTKKLPEVLVCANDSNAFAVITALKDMGLKVPKDI
                     SVVGFDDTPLCVKSVPQITTVQVQKELMGEVAVSNLIDRIHRKDNIPMTQLLSVKIVE
                     RTSLKI"
     gene            2321..>2526
                     /locus_tag="PLK68_11110"
     CDS             2321..>2526
                     /locus_tag="PLK68_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90992.1"
                     /translation="MIKKALRIILSLVMSFGLVLSSMNVTYASESDFATIKTRLKDYF
                     LTLDTIDDGSKVETCYVSKAKDYL"
BASE COUNT          891 a          286 c          487 g          862 t
ORIGIN      
        1 ataatgaaaa aggatttgtg ttttatgaac attttattga tgcactgctt aaaagaggaa
       61 ttcaaccaat tccaacttta tatcattttg aaatgccagc atttttatat gagaagtata
      121 atggatttta tagtcgcaaa gtagtcgata tatttgtaga actgtgcaaa aagattgttg
      181 atcgttatca tgataaagtt gaaaattgga ttatttttaa tgaacaaaat ggaattttac
      241 agaaagggcc aaaaatgttt tttggggcag tttgtccgga tggagttgat actcaaacgt
      301 ttgataatca aattatgcat aatactttga ttgctcatag tcttattaat gaatatattc
      361 atcaaaaagg tggtaaggtt atgggaatgg cgacagtggt tcaaagttat ccagaaactt
      421 gtcatcctct tgatacactt gaaagtatga aggctcaaag tgaagcttat gtttttttag
      481 atgtttttgc aagaggacat tataattcat attattttgc aaatatgaaa aatgaaggaa
      541 caatgcctga aatcttagat ggtgatttag aaatattaaa gaaagggatt acggattcac
      601 tatctattag ttattatatg tcgacaattt cacattatgg tgaggagagt ctaacaaata
      661 ttaatgatgt tgtaattaaa aagaatcctt atttggaaat gtcagaattt ggctggacga
      721 ttgatccaac tggtcttaga attactttaa gacaattgta tgatcgttat gaaatgccaa
      781 tatatatagt ggaaaatggt tttggttata atgatcaaat aaatgcagat ggccaaatta
      841 tcgatgatta tcgtattgat tatatgagga aacatcttga ggaaatgaaa ttagcgattc
      901 aagaaggtgt tgattgccgt ggttacttgt catggggacc ggttgacatt ttaagcagcc
      961 gggcacaaat gaagaaaaga tatggtttta tttacgtcaa tcgtgaaaat gatgatttaa
     1021 aagatatgcg tagaattaga aaaaaatcat ttgcctggta tcaacaagta attgcatcaa
     1081 atggagaagt actataatcg aggagcatgt gctatactat ttaatgaggt gaaacaaaga
     1141 tgaaagtgac gatgaaagat attgctaata aattaggaat ttcaattaat gccgtttcaa
     1201 ttgcactaaa tgataagcct ggtgtcagcg atgaaatgcg tttggaaatt ttaaaaatgg
     1261 ctgataaaat gggttacatt aatcaaaaaa gacaatatct atcagtttat tctaaatcaa
     1321 atatttgtat tttaatgcaa agttattatg cagatacagg acacttttat tcgattgttt
     1381 tacggtcaat cgttgaacaa gcaaaagtat ttggttattt ttcaatttta aattattttg
     1441 aagacgggca cttcgtagta cctgaatgta ttgaagaaag gaaagtggct ggaatattag
     1501 ttgtaggcaa aatatctgat agtaacctgc tgctgcttaa aaatattggt gtacccgttg
     1561 ttctagtaga ttttacatca ttatgtacac cgtgtgattg tgttttgacg cataataaac
     1621 aaggtagtta tatgatgact acgcatgtta ttaataaagg gtatcaacgg attggttttt
     1681 ttggagatct tgattattcc tttagttttg aggatcgttt tattggattt aaacagtcac
     1741 tgttaaagaa taatattgtt agttattcaa aggttgatga atatattcaa aaatattctt
     1801 ttctacaagg aattgaagaa tatgttttaa ctaatcaaat tgataaaata attgaaattt
     1861 tgaatactaa aaaattacca gaggtcttag tatgtgcaaa tgattctaat gcatttgctg
     1921 tgattactgc gttaaaagat atgggcttaa aagtcccaaa ggatattagt gtcgttggat
     1981 ttgacgatac accgttgtgt gttaaatcag taccgcagat cacaacagtt caagttcaaa
     2041 aggaactgat gggcgaggtg gcggtatcta atctaattga tcggattcat cgtaaagata
     2101 atattccgat gacacaatta ttaagtgtga aaatagttga acgaacgtct ttaaaaattt
     2161 aaagacgttt tcatcattta taattcaacc atattctact gattataaag gtttataaaa
     2221 aaactttttc aagtgatttt tcgttgaaat tcaagtgata ataactaaaa ataatagatg
     2281 tagtaagcgt tttcaatatt atttaaaagg aggatattat atgattaaaa aggcattgag
     2341 aataatcctg tcattagtta tgtcttttgg gcttgtttta agttccatga atgtaactta
     2401 tgcaagtgag agtgattttg caacaatcaa aacgcgttta aaagattact ttttaacttt
     2461 agacacgatt gatgatgggt caaaagtaga aacatgctat gtttcaaaag caaaagatta
     2521 tcttga
//
LOCUS       DAOMTA010000440         2524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1862_length_2524_cov_2.790603, whole genome shotgun sequence.
ACCESSION   DAOMTA010000440 DAOMTA010000000
VERSION     DAOMTA010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1862_length_2524_cov_2.790603"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..587)
                     /locus_tag="PLK68_11115"
     CDS             complement(96..587)
                     /locus_tag="PLK68_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM90993.1"
                     /translation="MGLFNKWKKKVEVEPYRISAPVAGKVIDIKDTNDQVFNSEALGK
                     GVGIIASEKQITAPIGGQIVSFFPTKHAVGIKTDKGVELLIHVGIDTVELNGKHFISM
                     KKQGDTVVRGDILLKVDFDAIREAGYDPTVLMVVTNTAQYAQIKCNLGNKSNNDIIIE
                     IEE"
     gene            complement(597..1532)
                     /locus_tag="PLK68_11120"
     CDS             complement(597..1532)
                     /locus_tag="PLK68_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM90994.1"
                     /translation="MSDVIRLISASGSDFEKMTPMELKESIFKSEGRVIMGQHLLFAG
                     EGLVRGITNSEVMFAFGADMVMLNTMDLDNMENNKGLQGLSYQTLRQRCKRPLGIYLG
                     CPKAGFEDGGKKALYRREGMLCTPEHVQKCVDMGVDFIVLGGNPGSGTSINDVIACTK
                     WIKEKYGDQLFVFAGKWEDGINEKVLGDPLANRDAKEIIKELIDAGADCIDLPAPGSR
                     AGISVTMIRELVEYIHSYKPGTLAMSFLNSSVEGADPDTVRLIALKMKETGADIHAIG
                     DGGFSGCTSPENIHQLSISIKGRPYTYFRMASVNK"
     gene            complement(1534..2184)
                     /locus_tag="PLK68_11125"
     CDS             complement(1534..2184)
                     /locus_tag="PLK68_11125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM90995.1"
                     /translation="MKKLLCPSMMCANFGNLENEIRKLEESGIDIFHLDVMDGSFVPN
                     FGMGLQDIEYICKTANKPCDVHLMVVNPGAYVKKFAQLGVKIIYVHPESDVHITRTLQ
                     MIKDAGAQAGIVVNPGTSYESVKETLSLVDYVMVMSVNPGFAGQKYLNFVDDKFKQFC
                     AKKEEFGYKVMIDGACSPERIAMLSSIGVEGFILGTSALFGKEKSYQEIIKELRGL"
     gene            complement(2208..>2524)
                     /locus_tag="PLK68_11130"
     CDS             complement(2208..>2524)
                     /locus_tag="PLK68_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIB"
                     /protein_id="HRM90996.1"
                     /translation="KNSQIRQNAMSAAVTGFFSITEPAIYGVNLKYKKPFYIAVGCAA
                     VSGGIAGFVNAAALAGGPVGVLSFPLFFGEGFVGFCIAMVFAFVASAVLTYLFGYDRS
                     ND"
BASE COUNT          781 a          590 c          298 g          855 t
ORIGIN      
        1 attacttcat agccatattt tgcttcaata tactctctga tcacattttt atatttaact
       61 gcaacatgat catttccaat tccaattttc attttctact cctctatctc aataataata
      121 tcattattac ttttatttcc taaattacat ttgatttgtg catattgagc agtatttgtc
      181 actaccatca agactgttgg atcgtaacca gcttctctaa ttgcatcaaa atcaaccttt
      241 agtaaaatat cgccacgtac aaccgtatca ccttgtttct tcattgagat aaaatgttta
      301 ccgtttaatt caacagtatc aattccaaca tgaatcaata attctacacc tttatccgtc
      361 ttaattccaa ccgcatgttt tgtaggaaaa aaacttacaa tttgaccacc gattggggca
      421 gttatttgtt tctctgatgc tatgatgcct actccttttc ccaatgcttc actattaaat
      481 acttgatcat ttgtatcttt gatatcaatt acttttccag ctactggtgc actaatacga
      541 tatggttcaa cttctacttt tttcttccat ttattaaata atcccattat tttcacctat
      601 ttattaacac ttgccattct aaaatatgta taaggtcgcc ctttaattga aatactcaat
      661 tggtgaatat tctctggtga agtacatccc gaaaagccac catcaccaat agcatgaata
      721 tcagcaccag tttctttcat ctttaacgca atcaagcgta ccgtatctgg gtcagcccct
      781 tcaactgaac tattcaaaaa gctcattgcc agagtgcctg gtttataact atgaatgtat
      841 tcaactagtt cccgaatcat cgtgacactg atgcctgctc tacttcctgg ggctggtaaa
      901 tcaatacaat cagctcccgc atcaatcaat tctttaatta tctctttagc atctcgatta
      961 gccagaggat ctccgagcac cttttcgttt ataccatctt cccattttcc agcaaagacg
     1021 aataattgat caccatactt ttccttgatc catttagtac aagcaattac gtcattaatt
     1081 gaagttccac taccaggatt accacccaaa acaataaaat caactcccat gtcaacacat
     1141 ttttgaacat gttcaggtgt acaaagcatt ccttcacggc gatataaagc ttttttacca
     1201 ccgtcttcaa atcccgcttt gggacaacct aaataaatcc caagtggcct tttacaacgt
     1261 tgccttaagg tttggtaaga taatccttga agtcctttat tattttccat attatctaaa
     1321 tccattgtat taagcataac catatcagca ccaaaagcaa acatcacctc agaattagtt
     1381 attcctctaa ctaatccttc accagcaaat aataaatgct gtcccatgat tacccgccct
     1441 tctgatttaa aaattgattc tttcaattcc atcggtgtca ttttttcaaa atcactgcct
     1501 gaagcactaa ttagtctaat tacatcgctc attttatagt cctctcaatt ctttaattat
     1561 ttcttgataa ctcttctctt taccaaataa agcactcgtt cctaagataa aaccttctac
     1621 cccaatactt gacagcatag caatccgctc cggcgaacat gccccatcaa tcatcacttt
     1681 atagccaaat tcctcttttt tggcacaaaa ttgtttaaat ttatcatcaa caaaatttaa
     1741 atacttttga ccagcaaaac ctggattaac actcattacc atcacataat cgacaagcga
     1801 taatgtttct tttacacttt cataagatgt cccaggattt actacgattc cagcttgcgc
     1861 tccagcatct ttgatcattt gcaaagttct agttatatgc acatcacttt caggatggac
     1921 atatataatc tttaccccta attgtgcaaa tttttttaca tatgccccag gatttactac
     1981 catcagatga acatcacaag gtttatttgc tgttttgcaa atatattcaa tatcttgcag
     2041 tcccattcca aaatttggaa caaaactccc atccattaca tcaaggtgaa aaatatcaat
     2101 tccactttcc tctaattttc ttatttcatt ttcaagatta ccaaaattag cacacatcat
     2161 tgatggacat aatagttttt tcatattttt tctcccctaa atgtttctta atcattactg
     2221 cgatcgtatc caaatagata agtcaaaaca gctgaagcga caaatgcaaa taccattgca
     2281 atacaaaatc ctacaaatcc ttcaccaaaa aataatggga atgataaaac tccaactggt
     2341 ccccccgcta aagctgcagc attaacaaat ccagcaatcc caccagaaac tgctgcacat
     2401 ccaactgcaa tatagaaagg ctttttatat ttcaaattga ccccgtaaat agccggttct
     2461 gtaattgaga agaatccagt tactgctgct gacattgcat tttgtcgaat ttgtgaattt
     2521 tttg
//
LOCUS       DAOMTA010000441         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1864_length_2523_cov_2.524311, whole genome shotgun sequence.
ACCESSION   DAOMTA010000441 DAOMTA010000000
VERSION     DAOMTA010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_2523_cov_2.524311"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1935)
                     /locus_tag="PLK68_11135"
     CDS             complement(217..1935)
                     /locus_tag="PLK68_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRM90997.1"
                     /translation="MSNYDNSPYIKINGYDNDAYSGYAQIDKKIKESLGNKKIVVVDC
                     YLGINDRELLNVLIKKLTPAHVILSEDIFYDGKKLTEMMQVNLTEDRVRGVMYYGTIR
                     DYVDEAKLAKIQKFVKNKEGIVLVYGFGASLITKGDLLIYADLTRWEIQLRYRAGLPN
                     FKQSNYDEDPLIKNKRGYFIEWRIADKHKREIFEDIDLYLDTNCSNKPKMITGIAFRN
                     ALRSVCNQPFRLVPYFDPGVWGGQWMKEVCNLDKSKSNYAWSFDGVPEENSINLKFGD
                     IVIATPALNVVMYQAIELLGAKNYARFGAEFPIRFDLLDTMGGGNLSLQVHPTTEYIK
                     SHFGMPYTQDESYYILDCDGGGVYLGLKENINSDEFINDLERANEGNQSFDADKYINF
                     FPAKKHDHFLIPAGTIHCSSANCVVLEVSATPYIFTFKLWDWDRVGLDGKPRPIHVED
                     GKKVIDYQRTTTWVESNLVNNFTEIEKSDEYTEVKTGLHELEFIETRVITTKQKIYQQ
                     TNKSVVMMNLVAGESAVIKSPKNKFEPYTVHYAETFIVPAQVGEFTIEPFDKNEIKVL
                     KAYVRN"
     gene            complement(1960..>2523)
                     /locus_tag="PLK68_11140"
     CDS             complement(1960..>2523)
                     /locus_tag="PLK68_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM90998.1"
                     /translation="DDNKIEVVGINYYQPLRVRQRETAWNPDKPFIPTAYYETYSPRG
                     IKMNFSRGWEIYPKGIYDMLKIIQNDYRNIECWITENGMGVMDEDKFKDASGQIQDSY
                     RIEFLSDHMAWMLKAIDEGCDCRGFLNWTFTDNLSPVNAFRNRYGFVEIDMENNRNRR
                     IKKSGDWVKELMRTRCFEAEDTEPEYK"
BASE COUNT          806 a          515 c          274 g          928 t
ORIGIN      
        1 tcgatcaaaa attcttttta taattaagac catgatactt ataataatca tgataaagac
       61 tatctgtcaa caagaataat tacttattta tacgatattt actacttttt ttgtcaattt
      121 aaagagaaat aattaaaaaa atagaattct attttaatat gctccgctta agtaaacaag
      181 gaacatatca ttttgaattc tatttcttta attaaatcag tttctaacat atgcttttaa
      241 tacttttatt tcatttttat caaacggttc aatagtaaac tcacctactt gagctggtac
      301 aataaaagtt tccgcataat gcactgtata tggctcaaac ttatttttag gacttttaat
      361 tactgcactt tccccagcaa ctaaattcat cattaccaca cttttattag tttgttgata
      421 aatcttttgt ttagttgtaa taactcgtgt ttcaataaat tctaattcat gtaatccggt
      481 tttaacttct gtatattcat cactcttttc aatttctgta aaattattta ccaaattgga
      541 ttctacccaa gtagttgttc tttgataatc aattaccttc tttccatctt caacatgaat
      601 cgggcgtggt ttaccatcta atcctacacg atcccaatcc cataatttaa aagtaaaaat
      661 atatggtgta gcacttactt ccaagactac acaattagcc gaagaacaat gaattgttcc
      721 tgctggaatt aagaaatgat catgcttttt tgctggaaaa aaattaatat acttatcggc
      781 atcaaaactt tgattaccct cattagctct ttctaaatcg ttaataaatt cgtctgaatt
      841 aatattctct tttaaaccta aatatactcc acctccatca caatcaagga tatagtagct
      901 ttcatcttgc gtataaggca ttccaaaatg gctcttgata tattcagttg ttgggtgtac
      961 ttgtaagctt aaatttcctc cacccattgt atcaagaaga tcaaatctaa ttggaaactc
     1021 tgccccaaaa cgagcataat tttttgctcc taaaagctct atagcttgat acataacaac
     1081 atttaaagct ggggttgcaa taacaatatc gccaaacttc aaattaatac tattctcctc
     1141 tggaacccca tcaaaactcc aagcataatt agatttactt ttatctaaat tacaaacctc
     1201 tttcatccat tgtcctcccc aaacccctgg atcaaaatat ggtactagac gaaatggttg
     1261 attgcaaaca cttcttaatg catttcgaaa cgcaatacca gtaatcattt taggtttatt
     1321 actgcaatta gtgtctagat ataaatcaat atcttcaaaa atctctcgtt tatgcttatc
     1381 ggcaattcgc cactcaataa aataaccacg cttatttttt attagaggat cttcatcata
     1441 attactttgt ttaaaattag gtaatccagc ccgatatctt aattgaattt cccatcgtgt
     1501 taggtctgca tatattaata aatcaccttt agtaattaga cttgccccaa aaccgtatac
     1561 aagaactatt ccttctttat ttttaacaaa cttttgtatc ttagccagct tagcttcatc
     1621 aacataatct ctaatagttc cataatacat tactccccta acacgatctt cagttagatt
     1681 cacctgcatc atttctgtca attttttacc atcataaaag atatcttcac ttaaaattac
     1741 gtgtgctgga gtcaattttt ttatcaatac atttaataat tcacgatcat taattcccaa
     1801 ataacaatct acaacaacta tctttttatt tcctaaagat tctttaattt tcttatcaat
     1861 ctgcgcatat ccagaatatg catcgttgtc atagccattt attttgatat aggggctatt
     1921 atcataatta ctcatttgct ttttgcctcc tactcaatct tatttatact caggttctgt
     1981 atcttcagct tcaaaacatc gtgttctcat taattctttt acccaatcgc ctgatttttt
     2041 aattctccga ttccgattat tttccatatc aatttccaca aacccataac gatttctaaa
     2101 tgcattaaca ggtgaaagat tatctgtaaa cgtccaattc aaaaagcccc gacaatcaca
     2161 accttcgtca attgctttta acatccaagc catatggtca cttaaaaatt caattcgata
     2221 gctgtcctga atttgcccgc tagcatcctt aaacttatct tcatccatta ctcccatacc
     2281 attttcagta atccaacatt caatatttcg ataatcattt tgaatgatct tcagcatatc
     2341 ataaattcct ttaggataaa tttcccatcc tcttgaaaaa ttcattttaa ttcctcgtgg
     2401 actatatgtt tcataatatg cagttggaat aaatggttta tctggattcc atgccgtttc
     2461 tctttgtcga acccgtaacg gctgataata gttgattcca acaacttcta ttttattatc
     2521 atc
//
LOCUS       DAOMTA010000442         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1865_length_2523_cov_1.828606, whole genome shotgun sequence.
ACCESSION   DAOMTA010000442 DAOMTA010000000
VERSION     DAOMTA010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1865_length_2523_cov_1.828606"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLK68_11145"
     CDS             <1..453
                     /locus_tag="PLK68_11145"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRM90999.1"
                     /translation="HFGIMHIFCNCYSLVNLGSVMEYLLGMKRYLIILIASALATTIL
                     PTVFYILTGNGASSIMGGISGAIFGLMGALLALAWKFKDVYAYLFKQISSSVLLMLLI
                     SILVPSISLSGHISGMIGGFIATLLIIKFMPLRIWKKKKTENYHNLVN"
     gene            496..1395
                     /gene="galU"
                     /locus_tag="PLK68_11150"
     CDS             496..1395
                     /gene="galU"
                     /locus_tag="PLK68_11150"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRM91000.1"
                     /translation="MKQKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDTPTIQYIIQ
                     EAVDSGIEEILIITNSNKHAMENHFDKSYELEARLTESGKMEQVKMIQDIADMANIYY
                     IRQKEPKGLGHAVLCAKSFIGDEPFAVLLGDDIVVNDGGEPALKQLIDAYINKEASVV
                     GVQTVEHKDVCKYGIVSPSHSHPRENGGRLVKLNNMVEKPAVEEAPSDMAVLGRYVLT
                     PKVFELLETQGKGAGGEIQLTDAIKRLMDIQAVYAYDFEGIRYDVGDKFGFIKATIDF
                     ALKREDLKEKVQAYINSLVKDVK"
     gene            1395..1862
                     /locus_tag="PLK68_11155"
     CDS             1395..1862
                     /locus_tag="PLK68_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91001.1"
                     /translation="MSFQEKYEEYKQRKEAQAFFNQNNDQKVYGKDYLIAILVGFGST
                     IVMGCILTWIISKIGFNFSYFTILIGIFEAQAIKKVLNKSGQQLAIIAAVTFVLGIVV
                     AQAIFISITLPFFNVSMLVETFKYCFQNMITGDVLSTIIYLFGAIAAYMALKD"
     gene            2105..>2523
                     /locus_tag="PLK68_11160"
     CDS             2105..>2523
                     /locus_tag="PLK68_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRM91002.1"
                     /translation="MAKKFLSMDGNTAAAHVAYAFTEVASIYPITPSSPMAENAEAWS
                     AQGKKNIFGSPVNVIEMQSEAGAAGAVHGALQGGALATTFTASQGLLLMIPNLYKIAG
                     ELLPGVFHVSARALATRALNIFGDHQDVMAVRQTGCA"
BASE COUNT          847 a          305 c          512 g          859 t
ORIGIN      
        1 cattttggaa ttatgcatat tttttgtaac tgttattcat tagttaatct aggctcagtt
       61 atggaatatt tgttgggaat gaagcgttat ttgattattt tgattgcttc tgcccttgca
      121 actacgattt taccaacagt tttttatatt ttgactggaa atggtgctag tagtattatg
      181 ggaggtattt ctggagctat ttttggattg atgggagctt tattagcatt ggcttggaaa
      241 tttaaagatg tttatgcata tttattcaag caaatatcgt caagtgttct attgatgctg
      301 ctgatttcta tattggtgcc ttcgatttct ttatctggac atatttcagg tatgattggt
      361 ggttttattg caactttact aattatcaaa tttatgcctt tacgcatttg gaaaaagaaa
      421 aaaacggaaa attatcataa tcttgtcaat taataatcag atatagtaaa atataaacat
      481 atagaggaga gtacaatgaa acaaaaagta agaaaagcaa ttattccagc ggctggatta
      541 ggaacaagat ttttaccagc aactaaagca ctagcaaagg agatgttgcc aattgttgat
      601 accccaacaa ttcaatatat tatccaagaa gcagtggata gtggaattga agaaattttg
      661 attattacta attcaaataa acatgcgatg gaaaatcatt ttgataagtc gtatgaatta
      721 gaagcgcgtt taacagaatc aggtaaaatg gaacaagtta aaatgataca agatattgct
      781 gatatggcaa atatttatta tattcgtcaa aaagagccaa agggattagg tcatgcggta
      841 ttatgtgcaa aatcatttat tggtgatgag ccatttgcag tattattagg tgatgatatt
      901 gttgtaaatg atggtggtga gccagcatta aagcaattaa tagatgcgta tataaataaa
      961 gaagcatcag ttgttggggt acagacagtt gaacataaag atgtttgtaa atatggaatt
     1021 gtcagtccgt cacattcgca tcctcgtgaa aatggtgggc gtttagttaa gttaaataat
     1081 atggtggaaa agccggcagt tgaagaagct cctagcgata tggcagtatt aggaagatat
     1141 gttttgacac caaaagtatt tgaattgcta gaaactcaag gaaaaggtgc tggtggagaa
     1201 atccagttaa cagatgctat caaacgttta atggatattc aagcggtcta tgcgtatgac
     1261 tttgaaggga ttcgttatga tgttggtgat aagtttggtt ttattaaggc aacgattgat
     1321 tttgcattaa agcgcgaaga tttaaaagaa aaggttcaag catatataaa ttcacttgta
     1381 aaggatgtta aatgatgagt tttcaagaga aatatgaaga gtataaacaa agaaaagaag
     1441 cgcaagcgtt ttttaatcaa aataatgatc aaaaagtata tgggaaagat tatttaatag
     1501 ctattttagt tggatttggt tcaacgatcg ttatgggatg tattttaacc tggattattt
     1561 ctaaaatagg ttttaatttt tcatacttta ctattttaat tggaattttt gaagctcagg
     1621 caataaaaaa agtattgaat aaaagtggtc aacaattagc cattattgct gcagttactt
     1681 ttgttttagg aattgtcgtg gcacaagcaa tttttataag tataacgcta ccttttttta
     1741 atgtcagcat gctagttgaa acattcaaat attgtttcca aaatatgata acaggtgatg
     1801 tgttaagtac aattatctat ttatttggtg cgattgcggc atatatggca ttaaaagatt
     1861 aaatatatta atttaaaaga atataattgg ctgatcggtt ttagtgatca gcttttttgt
     1921 tatattattt ttaataaaga ttgatggatt caaggtaaat ggattgttaa ataaaaacat
     1981 tctttttatt taaaatgaaa aaataatgcc tttctatatt tgtgagggtc ttttagtaat
     2041 tataactagc aatacaaaag gctacgtagt ataataatgg tatgcaaaag gaggattttt
     2101 aaaaatggct aaaaagtttt tatcaatgga tggtaatact gctgcagcgc acgttgcgta
     2161 tgcgtttact gaagtagcaa gtatttatcc aattacacct tcatctccaa tggctgaaaa
     2221 tgcggaagca tggtctgccc aaggtaaaaa aaatatcttt ggttcacctg ttaatgtaat
     2281 tgaaatgcaa tcagaagccg gagctgctgg tgctgtccat ggggcgttac aaggtggtgc
     2341 acttgcaact acttttactg cgtctcaagg tttattattg atgattccta acttatacaa
     2401 gattgctggg gaattattac ctggtgtatt ccatgtatca gcaagagcac ttgcaacaag
     2461 agcattaaat atttttggtg atcatcaaga tgttatggct gttcgtcaaa caggatgtgc
     2521 tat
//
LOCUS       DAOMTA010000443         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1866_length_2523_cov_1.636548, whole genome shotgun sequence.
ACCESSION   DAOMTA010000443 DAOMTA010000000
VERSION     DAOMTA010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_2523_cov_1.636548"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    248..257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(444..1502)
                     /locus_tag="PLK68_11165"
     CDS             complement(444..1502)
                     /locus_tag="PLK68_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM91003.1"
                     /translation="MDKKAKIVIADLDFDYIMPLQARLAKMYYNQVELEIITDKDYYK
                     EFLSSPKSIDILLVSEELYDNSLNRHKIDNIFVLVENDDNDKSLNQNLLYKYTSVKEI
                     LNIIFSKSKASLSYNTKNITRKTKVILVFSASGGVGKTTLSLGLSACLAKNYRSVLYM
                     EACRLNTFHYFMSDKEPIATNDVYAKLSMDDIPKYNLLKNYLKTELFSYVPPFKASLV
                     SLNLNYDIFEKIAVSFKKSNEFDCIVIDTDSVFDDYKARLLEIADKVLIVTKQTKSSA
                     FALKQLIVNMSGLNSDKYIIVCNDFCKDSENAVILPEYASSFSVNEYVNHIENCETKT
                     LENLSEEDTIKRLSFLMI"
     gene            complement(1511..2425)
                     /locus_tag="PLK68_11170"
     CDS             complement(1511..2425)
                     /locus_tag="PLK68_11170"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRM91004.1"
                     /translation="MIKNEKMMIYDEFVCNSNMSYVLRDNSVFSSTGYKVLQNQKNDC
                     FVKCCKMILNGRIQLYYITEEYIQLSKLILSIDADIFISITSDFISHIMSVKSNGFLS
                     CKNIDLSLEHIYVDPLTNHVRLIYLPLNVDLYSDMFQFERSLRTEICKAIKMSSSLNS
                     PQTNKLLKNLNDETVPIENLICSFGGGKEAGNIYLNKFGQNLVKQSMRLVAVNTPLPF
                     ELKVNRDEFTIGRKESQVDGLISFNKMIGRVHCKVNYIKGSYYITDLQSANGTYVNKV
                     RITPNEPCSVENGDIVRLANSEFRVEIM"
BASE COUNT          838 a          442 c          297 g          936 t
ORIGIN      
        1 aaagttaata aaaactcctc aaaaaacaaa tccttatgta atctaacatt gcattccaaa
       61 gatgttccca ctgtgagttt taagacatta tcttgaattt caatttcttt ataaacattt
      121 tggttagaca gtattatctt atatctattc accgttttat ctcctaccca ttaaaactta
      181 tcaaacttcc atctcttatt ccgaattcat taaggctctt atctccgcga attaaggcta
      241 taggatgnnn nnnnnnngct cagaagataa atagcattcc ttaatattag acacatctat
      301 tcctaattta aatgcagaat tcaacgcaat gactaaatca ttagctgaaa tatccaaagg
      361 tatttctaaa tcaaacgact cttttcgttc tgatatttcg aatacgataa ttgctttttt
      421 tgtttccata gttttctcca aatttatatc ataaggaaag ataatctttt aatagtatct
      481 tcctctgata aattttcaag cgtttttgtt tcacaattct ctatatgatt aacatattcg
      541 tttactgaaa atgaggatgc atattcaggt aaaataaccg cattttcaga atctttacaa
      601 aagtcattgc aaacgataat atatttatct gaatttaatc cggacatatt tacaattaat
      661 tgttttaaag caaatgcact ggatttcgtt tgtttggtaa cgattaaaac tttgtccgca
      721 atttctaata atcttgcctt gtagtcatca aatacactat ctgtatcaat cacaatgcaa
      781 tcaaattcat tcgatttttt aaatgaaacg gctatttttt caaatatatc ataatttagg
      841 tttaaagaca caagcgatgc cttaaaagga ggtacataac taaacaattc tgttttcaga
      901 taattcttca ataaattata cttcggtatg tcatccatac tcaattttgc atacacatca
      961 tttgttgcta ttggttcttt atcgctcata aaataatgaa atgtgttcaa tctgcaagct
     1021 tccatatata gaacagatct ataatttttt gccaaacacg ctgaaaggcc taacgataac
     1081 gttgtttttc caacaccccc acttgcagaa aacaccaata ttaccttggt ttttcttgta
     1141 atgtttttag tgttataact taaagaggcc ttgctcttac tgaaaataat attaagtatt
     1201 tcttttacgc ttgtatactt ataaagtaaa ttttgattca atgatttgtc attgtcatca
     1261 ttctcaacaa gtacaaatat attatcaatt ttatgtctgt ttaaagagtt atcgtatagt
     1321 tcttccgaaa ctaacaatat atcaatgctt ttgggagaac tcaaaaattc tttataatag
     1381 tccttatcag taataatttc caattcaact tgattgtaat acattttagc caatcgggct
     1441 tgtaaaggca taatgtaatc aaaatccaaa tcagctatta ctattttggc ttttttatcc
     1501 atagtacctc ctacataatc tctacacgaa actcactgtt tgccaaccta acaatatccc
     1561 cattttcaac actgcacggc tcattaggtg ttattctgac tttatttaca taagtaccat
     1621 ttgcactttg taaatcagta atataataac tgcctttaat gtaattaact ttgcagtgaa
     1681 ctctgcctat cattttatta aacgaaatta atccatctac ttgagattct tttctaccta
     1741 tagtaaattc atctctgttt acttttaatt caaaaggtaa cggagtattt acagccacta
     1801 aacgcattga ttgctttaca agattttgcc cgaatttatt caaataaata tttccggctt
     1861 ctttaccccc gccaaaactg caaattaaat tttcaattgg tacagtctca tcattcaagt
     1921 tttttaataa cttattagtt tgaggagaat taagtgaact tgacatctta attgccttac
     1981 atatttcagt tctcaatgat ctttcaaact gaaacatatc actatacaaa tcgacattga
     2041 ggggaagata gattagtctt acatgatttg ttaaaggatc aacataaatg tgttctaaag
     2101 aaagatctat gtttttacaa gataagaaac cgttactttt aacagacatt atgtgggaaa
     2161 taaaatccga agttattgat ataaagatat cagcgtcaat tgacaatatc agctttgata
     2221 attgtatata ttcttctgtt atgtaatata gctgtattct tccgtttaat atcatcttgc
     2281 aacattttac aaaacaatca tttttctgat tttgcagtac tttatatccg gttgaagaaa
     2341 acaccgaatt gtctcttaat acatacgaca tattggaatt acaaacaaat tcatcataaa
     2401 tcatcatttt ttcgtttttt atcatactca cttccgcctc aaaatttatt tagttttcac
     2461 attcaaattc aatttaatag tgaatttgag tctaaaaaca aatcacattc ttcacttgta
     2521 ttt
//
LOCUS       DAOMTA010000444         2522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1869_length_2522_cov_2.319822, whole genome shotgun sequence.
ACCESSION   DAOMTA010000444 DAOMTA010000000
VERSION     DAOMTA010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_2522_cov_2.319822"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLK68_11175"
     CDS             complement(<1..479)
                     /locus_tag="PLK68_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91005.1"
                     /translation="MLWQNKSYDLVSYLEIIIGIIALICLLSLLYLWYQPKCQNKFNN
                     IVFLGHLIICFDLYLLVDKVAHLLMSFYQAYLHHLKYNIYDYALLSSDILLTVVLIFS
                     TFLFINNLEHLLIKKDSKEEYNLILNRVFKAYNCLMVVFIFKDVVLKIVYDFLVDVRG
                     "
     gene            complement(463..1071)
                     /locus_tag="PLK68_11180"
     CDS             complement(463..1071)
                     /locus_tag="PLK68_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91006.1"
                     /translation="MQSFLAMHINNIVMILLLFIYVVCLNGLFSMRRRLIKWRRFFAF
                     LFIISTAGLMLGTIRNELMVYWFNNASFNIESNFEWMIKIGYDLISLIIMILMIFLVK
                     NILDFIKNSSRDAIRKLAINADIIAILIIIRTVIVPLIVKLARVLELFGLNNQTSSSN
                     DIFLYALPYLGLPEVAIIVVTIFINILGISYWNRGAEDVMAK"
     gene            complement(1164..2444)
                     /locus_tag="PLK68_11185"
     CDS             complement(1164..2444)
                     /locus_tag="PLK68_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRM91007.1"
                     /translation="MDNKNYGFETLQIHAGQKPDVTTKATGMPLYLSNAFTFEDADQA
                     ARIFALEEGGYFYSRLSNPTVDALQQRMAALDGGFGAVAFASGTAAIMGLIMTVCETG
                     DEIVAANNLYGGTIGSLSGTMAGMGFKTHFINPTDLEALKAAINDKTKIIFVESVGNP
                     NGDMLDFEAISAIAKEYQILFVVDNTTPTPYLFKPIEYGADLVVYSTTKYLAGHGNVM
                     GGVIVDSGNFKWKDNPRYPLFNMPDKAYHDIVYADLGAGAFCTKAVAKTLRDLGGCMS
                     PFNAYMTLLGLETLSLRMKKHVENARLLAKFLNECDDVEWVSYAELPDSPNYELAKKY
                     FPNGFGGLYTFGVKGGVAGGKTVINNIKLFIHVTNFADSRSLLTHPASTTHAQMSEEE
                     RIAGGVKQETIRMSVGLEDVEDLIADLKQAFAKI"
BASE COUNT          927 a          489 c          302 g          804 t
ORIGIN      
        1 cctctaacat ctaccaagaa atcatataca atttttaata caacatcttt aaaaataaat
       61 actaccatca aacaattata ggccttaaaa actcgattta aaattaaatt atactcttct
      121 ttgctatctt tctttattaa taaatgttct agattattga taaacaaaaa agtactaaaa
      181 attaaaacca ctgttaataa aatatcactg cttaataatg cataatcgta aatattgtat
      241 tttagatgat gcagataagc ctgataaaag ctcattaata aatgggcaac tttatctact
      301 aataaataaa gatcaaaaca aattatcaag tgtcctaaaa aaacaatatt attaaacttg
      361 ttttgacatt tgggttggta ccacaaataa agtaatgata gcaaacaaat caaggcaata
      421 atacctatta ttatttcgag ataactaact agatcataac ttttattttg ccataacatc
      481 ttcagcacct cgattccaat aacttattcc tagtatattg ataaagattg taacaacaat
      541 aattgcaacc tccggtaatc ccaagtatgg caaagcataa agaaatatat cattagagct
      601 gctagtttga ttatttaatc caaataattc caatactcta gctagtttaa caatcagcgg
      661 aacgattaca gttctaataa taattaagat agctataata tcggcattaa tagctaattt
      721 acgaattgca tcacgactac tatttttaat aaaatctaaa atatttttca ccaaaaaaat
      781 cattaaaatc ataataatta aactaatcag atcataacct atcttaatca tccactcaaa
      841 attgctctca atattaaaac tagcattatt aaaccaataa accatcagct cattacgaat
      901 ggttcctaac attaaacccg cggtactaat aataaataag aacgcaaaga aacggcgcca
      961 ttttataaga cgacgtcgca ttgaaaatag tccatttaaa cagactacat agataaaaag
     1021 cagcaggatc ataactatat tattaatatg catggctaaa aaactctgca tttaatcacc
     1081 tcattaaaat tatacatgag gactaataaa aaaactagaa aaaaggctat tgttttgaac
     1141 aataaacaat agtcctaatt attttatatt ttagcaaatg cttgttttaa atccgcaatt
     1201 aaatcttcaa catcttcaag accaacagac attctaattg tttcttgttt taccccacct
     1261 gctattctct cttcctctga catttgagca tgtgtcgtac ttgcaggatg agtcagcaat
     1321 gaacgtgaat cagcaaaatt agtaacatga ataaataatt taatattatt aattactgtt
     1381 ttaccaccag caacaccacc tttgactcca aaagtatata atccaccaaa accattgggg
     1441 aaatattttt tagctagttc atagttagga ctatctggta attccgcgta actaacccat
     1501 tcaacatcat cacactcatt taaaaatttt gctaataatc gggcattttc aacgtgtttt
     1561 ttcattctta atgataatgt ttctaatcct aataaagtca tatacgcatt aaacggtgac
     1621 atacaccctc ctaaatcacg taatgtcttt gctactgctt tagtacaaaa agcacccgct
     1681 cctaaatcag cataaactat atcatgataa gctttgtcag gcatgttaaa taatggatag
     1741 cgaggattat ctttccattt aaagttccca ctatcaacga tcacaccacc cataacatta
     1801 ccatgaccag ctagatattt agtagttgaa tagactacta agtcagctcc atattcaatt
     1861 ggtttaaata aataaggggt tggtgttgtg ttatcaacaa caaataaaat ttgatattct
     1921 ttagcaatag cactaattgc ttcaaagtct aacatatctc cattaggatt tcctaccgat
     1981 tcaacaaaaa taatttttgt tttatcgttg attgccgctt ttaaagcctc taaatcagtt
     2041 ggattaataa agtgtgtctt aaatcccatt cctgccatcg ttcctgataa agaaccaatc
     2101 gtaccaccat acaaattatt tgcagcaaca atttcatctc cagtttcaca cacagtcata
     2161 atcaatccca taattgctgc agttccagaa gcaaatgcta cagccccgaa tccgccatct
     2221 aaagccgcca ttctttgttg tagagcatca actgttggat tagataatcg agaatagaag
     2281 tatcctcctt cctctaacgc aaaaattcgc gccgcttgat cagcatcttc aaatgtaaaa
     2341 gcatttgata aatataaagg catccccgta gccttagttg tcacatctgg cttttgacca
     2401 gcatggattt gtaatgtttc aaaaccatag tttttattat ccatagtatc tcctcctaaa
     2461 attgataaaa ttgtatcaca ccttgagctt tttttccata taaaaaggtt ggtctcccaa
     2521 cc
//
LOCUS       DAOMTA010000445         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1872_length_2520_cov_2.059229, whole genome shotgun sequence.
ACCESSION   DAOMTA010000445 DAOMTA010000000
VERSION     DAOMTA010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1872_length_2520_cov_2.059229"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..285
                     /locus_tag="PLK68_11190"
     CDS             76..285
                     /locus_tag="PLK68_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRM91008.1"
                     /translation="MYGGTNLISQLIEKDFIDRYHMTIIPTILRKEIRLFETTHDYEI
                     KLKLIKSLSYNGISDCFMNEDNILL"
     gene            complement(394..1662)
                     /gene="eno"
                     /locus_tag="PLK68_11195"
     CDS             complement(394..1662)
                     /gene="eno"
                     /locus_tag="PLK68_11195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM91009.1"
                     /translation="MPYISNVYARQVLDSRGFPTIQVEVTTESGFSGSAIVPSGASTG
                     KYEALELRDNDDARYRGKSVFKAINNVNETIAKLIKEKSVLEQREIDLAMIRFDNTEN
                     KEKLGANAMLAVSLAVANCAANYLEIPLYRYLGGCNAHVLPTPMINIVNGGSHATNSL
                     DFQEFMIMPISANSFYQAMEMATNVFHTLKDILKRSNLATSVGDEGGFAPNLESNDDA
                     LELIIKAIKECNYEPGKDISIALDVAASELYQNGIYTINGKQYTSDELIKYYEQLVSK
                     YPIISIEDGLDQEDYTGWKKLTSRLGAKIQLVGDDLFVTNTNRLKAGIAGNYSNSILI
                     KLNQIGTLSETIDCIEMAVKNQIATIISHRSGESEDTFISDLAVGLNIGQIKTGSMSR
                     SERICKYNRLLKIEDHLVGNSCYQGKINNK"
     gene            1885..>2520
                     /gene="murI"
                     /locus_tag="PLK68_11200"
     CDS             1885..>2520
                     /gene="murI"
                     /locus_tag="PLK68_11200"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRM91010.1"
                     /translation="MKINEEKNLPIGFIDSGLGGLSVLKEAIKIMPHEDFIYYGDSLN
                     APYGTKSVDEIRDLTFAIVDKLLKLGIKGLAVACNTATSAAVRQLRIMYPDLTIVGIE
                     PAIKPAVESNHGGEILVMATPMTIKQEKFNRLLDIYKDRAKIIPVSCKGLMEFVEHGN
                     LNGSFLEAYFNETLVPYLNDTTETIVLGCTHYPFLRPYLKEFLGDRKIQIID"
BASE COUNT          857 a          374 c          395 g          894 t
ORIGIN      
        1 gcaacaaaaa gaagttttgc attttgattt tgatttttaa atcttttaaa atcataagta
       61 ggaaaaaata tctggattta tggaggcaca aatctgattt cacaattaat tgaaaaagat
      121 tttatagatc gttatcatat gacgattatt cctacaattt taagaaagga aattcgtctt
      181 tttgaaacta ctcatgatta tgaaataaaa ctaaagttga ttaaatcact aagttataat
      241 ggaataagtg attgttttat gaacgaagat aacattttat tataaaagaa tttatatttt
      301 tgttaaaaat ggtagtgatg ttattgataa tatcgctacc atttttaaat taaaactaat
      361 taagttattt aattgaatta tttaagagat aaattattta ttattgatct ttccttgata
      421 acatgaatta ccgacaagat gatcttcaat ttttaataga cgattatatt tacaaatacg
      481 ttcactacga gacatcgaac cagtcttgat ttgtccaata tttaaaccga ctgccaaatc
      541 actaataaaa gtgtcttcac tttcaccaga acggtgtgaa ataattgtag caatctggtt
      601 ttttacagcc atttcaatac aatcaatagt ctcacttaaa gtaccgattt gatttaattt
      661 aattaagata ctattgctat agtttcctgc gattccggct tttaaccgat tggtattagt
      721 aacaaataaa tcatcaccaa ctaattgaat tttagctcct agtctactag taagtttttt
      781 ccaaccagta taatcttctt gatctaaacc gtcttcaata gagataatag ggtatttgct
      841 tacaagttgt tcgtaatatt taattaattc atcacttgta tattgtttac cattaattgt
      901 gtaaatacca ttctggtata gttcactggc agctacatcg agggcaattg aaatatcttt
      961 gccaggttca taattacatt ctttaatagc tttaatgatt aattctaggg catcatcatt
     1021 ggattcgaga ttaggcgcaa atccaccttc atcacccaca gaagttgcta gattacttct
     1081 tttcaaaata tcttttaaag tatggaaaac atttgttgcc atttccattg cttgatagaa
     1141 agaattggca ctgattggca taatcatgaa ttcttgaaaa tctagtgagt tagtagcatg
     1201 gcttccgcca tttacaatat tgatcatcgg ggttggtaag acatgagcat tgcaaccgcc
     1261 taaataacgg tataatggaa tttcaagata gtttgcagcg caatttgcaa ccgccaaaga
     1321 tactgccagc atggcattag cacctaattt ttctttattt tctgtattgt caaagcgaat
     1381 cattgctaaa tcaatttctc gttgttctaa tacacttttt tctttaatta atttggcaat
     1441 agtctcattc acattgttta ttgctttaaa tacacttttt ccacgatacc gtgcatcatc
     1501 gttatcgcgt aattctaagg cttcatattt tcctgttgag gcaccacttg gaacaatcgc
     1561 acttccagaa aatccacttt ccgtagttac ttcaacttga attgttggaa aacctcttga
     1621 atctaatact tgtcttgcat atacatttga aatatatggc ataattttat cactccttca
     1681 ttttttatta tgaacggctt taatttgctt taatcaaata tattatcttg tttttaataa
     1741 ttattatacg gtaataaagg agttttttat taatgattca tgtaattgtt aattcaaagg
     1801 tactttacaa gattggatac aagtgctaat atgcaattaa ttttagagat gttaatcaag
     1861 tgctagataa aggggagaac acgaatgaaa ataaatgaag aaaaaaattt accaattgga
     1921 ttcattgatt ctggtttagg gggattaagt gttttaaaag aggcgataaa gataatgcct
     1981 catgaagatt ttatatacta tggtgattct ttaaatgctc cttatggaac caaatcagta
     2041 gatgagatta gagatttaac ctttgctatc gttgataaat tattaaaatt agggatcaaa
     2101 ggattagcag tagcgtgtaa tactgcaaca agtgcagctg ttcgacagct tagaataatg
     2161 tatcctgatt taacaatagt tggaattgaa ccagcaatta aaccagcagt tgaatcaaac
     2221 catggtggtg aaatcttagt aatggcaaca cctatgacaa taaaacaaga aaagtttaac
     2281 cggctgctag atatctataa agaccgtgcc aaaataatac cagtatcatg taaggggtta
     2341 atggaatttg tagaacatgg aaatttgaat gggagctttt tagaagcata ttttaatgaa
     2401 acacttgtcc cttatttaaa tgatactact gaaacaattg ttttaggatg tacgcattac
     2461 ccctttttaa gaccgtattt aaaagaattt ttaggagatc gaaaaataca gattattgat
//
LOCUS       DAOMTA010000446         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1874_length_2520_cov_1.565517, whole genome shotgun sequence.
ACCESSION   DAOMTA010000446 DAOMTA010000000
VERSION     DAOMTA010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_2520_cov_1.565517"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..>425
                     /locus_tag="PLK68_11205"
     CDS             144..>425
                     /locus_tag="PLK68_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91011.1"
                     /translation="MMLNGIQIPESLNKLPEDSIPNNTQIGITANIRRIVARYGVDDD
                     EWAEAISSQYFLLCLSNKLKQKNEDYENLLNKCLSSFRVTYKGQFVLDKD"
     assembly_gap    426..463
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            471..>727
                     /locus_tag="PLK68_11210"
     CDS             471..>727
                     /locus_tag="PLK68_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91012.1"
                     /translation="MKNEDSNDSYFRRSAKTRADNIDSQRMYINFHYGLLIRIVSSED
                     TEVLRAIKHTCLDNLLNNKSVDPYGGWYPYRVPWITSRILI"
     assembly_gap    728..737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <738..1222
                     /locus_tag="PLK68_11215"
     CDS             <738..1222
                     /locus_tag="PLK68_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91013.1"
                     /translation="SEKEKVDKVVTDAVSSIYQRINEKAPYWRSGVGNWVSKWESTAL
                     CLEALYVWEAIDTKKDLIEKVIRYIFNEENKSEWLNTNISFETEDSANKVLASVILAS
                     VSFRVTKTYYKDIYLKHADEIIDFLKRVISVILNQDIKAVLQYCTVPQILYYVLSAIE
                     "
     gene            1237..2061
                     /locus_tag="PLK68_11220"
     CDS             1237..2061
                     /locus_tag="PLK68_11220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM91014.1"
                     /translation="MSGWNKNLASIWTNNVAPSRPSNAEMCVYTKYLRELQSQCGQRI
                     KMLVLGSTPEFRDWGFEQPIDISVVDKSIEYHKCISREIRHKNIKESVYVQNWEEMTF
                     DKQFDLIIGDLAIGNVDPDLFHVFLQNVNKSLSKNGFFLGKSLIWDENELIKSPTEII
                     DDYRLQNYIHPYTFINHRLGLYCLDRNTNTLDFSIMFNELKKLYENGYIDQQLFSYFT
                     DVGWSTEMKFKFFAPTQKQFIDCVNQELQFVKFEHTEDCYTNVFPIYVIKKGGYFK"
     gene            2058..>2520
                     /locus_tag="PLK68_11225"
     CDS             2058..>2520
                     /locus_tag="PLK68_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM91015.1"
                     /translation="MNIFLVHGSFGKPFENWFPWLEEELSKRDIVCTIPTFPTPKHQN
                     YKDWEKLMNYYCELEIVNSETVLIGHSCGCAFLVKYLLAHKIRVKGLITVSGYNDFFG
                     GDNIMDSLNKPFYFVLPSSIDMNNYAKTIISYYGNDDPYIPQKYLSSFAHLI"
BASE COUNT          906 a          316 c          443 g          807 t
ORIGIN      
        1 atagaaatag aatgctataa aattatagcc acaggaaatg attttataga ggacaatcca
       61 gttataaaca ccttgcataa aaatcgagtg gagttatcta aacagatcga taagggattg
      121 agattattga aaggtgggtg acaatgatgt taaatggaat tcaaattccg gaaagtttaa
      181 ataaattgcc tgaagacagc ataccaaata acactcagat tggtattact gctaatataa
      241 gacgtatagt tgcaagatat ggcgtcgatg atgatgaatg ggcagaggct atatcttcac
      301 aatattttct gttatgtctt tcaaataaat tgaaacagaa aaatgaagat tatgaaaact
      361 tgttaaataa atgtttatca agttttagag ttacttataa aggacaattt gtattagata
      421 aagacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgaatgt atgaaaaatg
      481 aagacagtaa tgattcatat tttagacgtt cagccaaaac acgagccgat aacatagata
      541 gtcagcgaat gtatattaat tttcattacg ggttattgat tcgtatagta tcatcggaag
      601 atactgaagt tctgcgtgca ataaaacata catgtttaga caatctttta aacaataaat
      661 cggttgatcc atatggaggt tggtatcctt accgtgtacc atggattact tctcgaatat
      721 taattagnnn nnnnnnnatt ctgaaaaaga aaaagttgat aaagtagtaa ctgatgcagt
      781 atcttcaatt tatcaacgaa taaatgaaaa agctccatac tggcgaagtg gcgttggtaa
      841 ttgggtttcc aaatgggaat caacagcatt atgtttagaa gcattatatg tatgggaagc
      901 cattgacacg aagaaagatt taatagaaaa ggtaatcaga tacatcttta acgaagaaaa
      961 taaaagcgaa tggttaaata ctaatattag ttttgaaacc gaagactccg caaataaagt
     1021 tttggcatct gtaatactcg catcagtatc attccgtgtt acaaaaactt attataaaga
     1081 catatatttg aaacatgctg atgaaataat agattttttg aagcgtgtta tatccgtaat
     1141 tttaaatcag gatataaagg ctgtattgca atactgtaca gttcctcaaa ttctttatta
     1201 tgttctatct gcaatcgaat aaaggaggtg atttccgtga gtggatggaa taaaaattta
     1261 gcatctattt ggacaaacaa tgtggctcct tctcgtccgt caaatgctga gatgtgtgta
     1321 tacacaaaat atttgcgtga attacagtcc caatgtggtc aaagaataaa aatgcttgta
     1381 ttggggtcta caccagagtt tagggattgg ggatttgagc aaccaataga tataagtgtt
     1441 gttgataaga gcatagaata tcataaatgc atatctagag aaattaggca taaaaacatt
     1501 aaagaatcag tatatgtaca gaactgggaa gaaatgactt ttgataagca gtttgattta
     1561 attattggag atttggcaat aggtaatgta gatcctgatc tatttcatgt ttttcttcaa
     1621 aatgtaaaca aatcactttc aaaaaatggt ttctttcttg ggaaaagctt aatttgggat
     1681 gagaacgaat taataaaatc gccaacagaa ataattgatg attatcgtct tcaaaattat
     1741 atacatccat atacatttat taatcatcgt ttaggtttgt attgccttga tagaaacacg
     1801 aatacattag attttagcat tatgtttaat gagctaaaaa agctatatga aaatggctat
     1861 atagatcaac aattattttc atatttcaca gatgtggggt ggagtactga aatgaaattt
     1921 aagtttttcg cccctacgca aaaacaattt attgattgcg taaatcagga attacaattc
     1981 gtaaaatttg agcatactga agattgctat acaaatgtat ttccaattta tgtaataaaa
     2041 aaaggaggat attttaaatg aatatttttt tagtacacgg ttcttttggt aagccttttg
     2101 aaaattggtt tccatggtta gaagaagaat tatcaaaaag ggatattgta tgcacaattc
     2161 ctacttttcc tactccaaaa catcaaaact ataaggattg ggaaaaacta atgaattatt
     2221 attgcgaatt agaaatagtt aattcagaaa cagttctaat tggtcatagt tgtgggtgtg
     2281 cttttttggt taagtattta ctggcacata aaataagagt aaaaggacta attaccgtat
     2341 ctggttataa tgactttttt ggcggtgata atattatgga ctctttaaat aaacctttct
     2401 attttgttct tccttcttca atagatatga ataattatgc aaaaactata atctcatatt
     2461 atggaaatga tgatccttat attccacaaa aatatcttag cagttttgct catctaatag
//
LOCUS       DAOMTA010000447         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1880_length_2517_cov_2.244110, whole genome shotgun sequence.
ACCESSION   DAOMTA010000447 DAOMTA010000000
VERSION     DAOMTA010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_2517_cov_2.244110"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLK68_11230"
     CDS             <1..974
                     /locus_tag="PLK68_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM91016.1"
                     /translation="LDLDEIMKKYSPGHNDATRNTSLEMIQEVAQQNPTFMSGTADLA
                     SSTKTNIAGEKTFSVENYDGRNLAFGIREFAMVAMMNGITLHTGIKVSAGGFLVFSDY
                     FKGALRMACLMELPIILPLSHDSIAVGEDGPTHQPVEQLTMLRSMPNMRVFRPADAVE
                     MASAWKLAVESTNNPTALILTRQNVTTMTATSYEGVCHGAYIVGKEVNKLDAIIIASG
                     SEVNLAMAAKEELLKEDIDVRVVSMPSMELFEQQDAKYKDEILPHNVRARLSIEMASD
                     FGWHKYVGLDGKTMSVNKFGASAPADIVIKNYGFTVENVVKNVKDIL"
     gene            1077..1457
                     /locus_tag="PLK68_11235"
     CDS             1077..1457
                     /locus_tag="PLK68_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation inhibitor of replication protein
                     SirA"
                     /protein_id="HRM91017.1"
                     /translation="MNTYKIYKLNPNVVGLLEQYPEVKNQIINIEPKNVFFTKQMECL
                     FGNNEGVSDFIEYKLKDRYDYHRDKNKHVIDNQLTKEEMICVIHDYYIIIEANKKTNI
                     FLDILYQISKSYVIMVEQSKNLEV"
     gene            1460..1660
                     /locus_tag="PLK68_11240"
     CDS             1460..1660
                     /locus_tag="PLK68_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HRM91018.1"
                     /translation="MLNIILGLIVGAIIGFFAARYMFKKQLQKNPPINEKMIRAMYME
                     MGRKPSEAQIKKIMSSVMSQYK"
     gene            1684..2304
                     /locus_tag="PLK68_11245"
     CDS             1684..2304
                     /locus_tag="PLK68_11245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM91019.1"
                     /translation="MKIYLTRHSKTLWNQEKRLQGWQDSPLTAEGIEDAKLLKARITE
                     LKIDYCYSSPIERAKATSEILFDHVLVDERLKEMNFGKYEGCLINELLNDPIYNRLWN
                     LPDDDVSTPGGETYHEVQMRLKDFFNDIYKKHHDDTIFITIHGMLFIILHGIMLNYKT
                     SELTKINKYVVRGCSLSEVEYDGKEFKIKYIGDDSHLKSSEIITYK"
BASE COUNT          929 a          330 c          437 g          821 t
ORIGIN      
        1 cattagattt agatgaaata atgaaaaaat attcacctgg tcataacgat gcaacaagaa
       61 acacttcatt agaaatgatt caagaagttg ctcaacaaaa tcctacattt atgtcaggaa
      121 cagctgattt agcatcttca actaaaacaa atattgcagg cgaaaaaaca tttagtgttg
      181 aaaattatga tggccgtaat ttagcgtttg gaattcgtga gtttgctatg gttgcaatga
      241 tgaatggaat cactttacac acaggaataa aagtatctgc aggtggtttc ctagttttct
      301 ctgactactt taaaggcgct ttgagaatgg cttgtttgat ggaattgcca atcattttac
      361 cattatctca tgactcgatt gcagttgggg aagatggacc aactcatcaa cccgttgaac
      421 aactaacaat gttacgttca atgccaaata tgcgagtatt tagaccagca gatgctgtcg
      481 aaatggcatc tgcatggaaa ttagcagttg aatcaactaa taatcctaca gctctaattt
      541 tgactcgtca aaatgttacc acaatgactg caacttcata cgaaggtgtt tgtcatggag
      601 cctacattgt aggtaaagaa gtcaataaat tagatgcaat tattatcgct tcaggtagtg
      661 aagtaaattt agcaatggca gctaaagaag aattattgaa agaagatatc gatgttcgtg
      721 ttgtatcaat gccatcaatg gagttatttg aacagcaaga tgctaaatat aaagatgaga
      781 ttcttccaca caatgttaga gctcgtttat cgattgaaat ggccagtgat tttggttggc
      841 ataaatatgt tggtttagat ggtaaaacaa tgtcagtaaa taaatttggt gctagtgcac
      901 ctgctgatat cgttattaaa aactatggtt ttacagttga aaatgtagta aaaaacgtta
      961 aagatatttt ataaggacta gtaaaagtga tgtaataaca tcacttttat tgatttttgg
     1021 gcatgatttg acaatatatt tagattttct ttgatacact aatcatggtg atagaaatga
     1081 atacatataa aatatataag ttaaatccta atgttgttgg attacttgaa caatatccag
     1141 aggtgaaaaa tcaaatcatt aatattgaac ctaaaaatgt tttttttacg aagcaaatgg
     1201 agtgtttatt tggaaataat gaaggagtta gtgactttat cgaatacaag ttaaaagacc
     1261 gatacgatta tcatcgggat aaaaataaac atgttattga taatcaatta acaaaagaag
     1321 aaatgatttg cgttattcat gattactata taattattga agcaaataaa aaaacaaata
     1381 tattcttgga tattttatac caaatatcaa aaagttatgt tataatggta gagcaatcta
     1441 aaaacttgga ggtataaata tgttgaatat aattttgggt ttaatcgttg gtgcgattat
     1501 cggttttttc gcagcacgtt atatgtttaa aaaacaatta caaaaaaatc caccaatcaa
     1561 cgaaaaaatg attcgtgcaa tgtatatgga aatgggtcgt aaacctagtg aggctcaaat
     1621 taaaaaaatc atgtcatctg ttatgtcaca atataaataa ggggtaaccc ttatttttac
     1681 tttatgaaaa tataccttac tcgacactca aaaacattgt ggaatcaaga aaaaagatta
     1741 cagggatggc aagattcacc gctaacagca gaaggcatag aagatgctaa gttgctaaaa
     1801 gcaagaatca cagaacttaa aattgattat tgttatagca gtccgataga acgtgctaaa
     1861 gcaacaagtg aaatattatt tgatcatgtt cttgttgacg aacgattaaa agaaatgaat
     1921 tttggtaaat acgaaggatg ccttattaat gaattattaa atgatccaat ttataatcga
     1981 ttatggaatc ttccggatga tgatgttagc actcctggtg gtgaaacata tcatgaagtc
     2041 caaatgcggt taaaagattt ttttaatgat atttataaaa aacatcatga tgatacaatt
     2101 tttattacga ttcatgggat gttatttatt attcttcacg gaataatgct taattataaa
     2161 acatccgaat taacaaaaat caataaatat gttgttcgtg gttgttcttt aagtgaagtt
     2221 gaatatgatg gaaaagaatt taagattaaa tatattggtg atgatagtca tctaaaaagt
     2281 agtgaaataa ttacatataa ataattacct aatggatgca tctctttttg gataatgcat
     2341 ccttattttt attaaataaa aaagccgtga taattcacaa ctttatgtat taacgagcat
     2401 atttacatcc aagtaatttt attttctgtc ccatttttaa ttctaattaa attttctttg
     2461 tgtcgataaa ttaacagtaa aataaccgct gcagttagga ttttaccagt cattgac
//
LOCUS       DAOMTA010000448         2516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1882_length_2516_cov_1.956522, whole genome shotgun sequence.
ACCESSION   DAOMTA010000448 DAOMTA010000000
VERSION     DAOMTA010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1882_length_2516_cov_1.956522"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..>941)
                     /gene="miaB"
                     /locus_tag="PLK68_11250"
     CDS             complement(83..>941)
                     /gene="miaB"
                     /locus_tag="PLK68_11250"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRM91020.1"
                     /translation="YGCNKFCTYCIVPYTRGKERSRLAKDIIKEVEELVAEGYQEITL
                     LGQNVNSYGKDLGEDYNFSNLLEDVAKTNIPRIRFTTSHPWDFSEDMIKIIAKYDNIM
                     PAIHLPVQSGNNEVLKLMGRRYSREQYLELFHKIKEYIPDCTVTTDIIVGFPNETHEQ
                     YLDTLSLYQECEYDLAYTFVYSPRAGTPAAKMVDNVASDEKDQRLYKLNEIVNEKAYK
                     QNQRFLNKIVEVLVEGTSKKDDSMLTGYTRHQKLVNFKGDPKDIGKIIKVKITEAKTW
                     ALKGESIES"
     assembly_gap    942..983
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<984..1424)
                     /locus_tag="PLK68_11255"
     CDS             complement(<984..1424)
                     /locus_tag="PLK68_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608890.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRM91021.1"
                     /translation="MVKVGLGKKYYIQTYGCQANERDSETLSGILESMSYQPTTEIKE
                     ADVIVLNTCAIRENAEEKVFGKVGYVKNLKKTNPNLIFAMCGCMAQEEVVVNRILEKH
                     PHVDLIFGTHNIHRLPELLKDALYSKEMIVEVWSKEGDVIENAPV"
     gene            complement(1649..1894)
                     /locus_tag="PLK68_11260"
     CDS             complement(1649..1894)
                     /locus_tag="PLK68_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91022.1"
                     /translation="MAKCKLLMQTAQTQKLIDFFDGYEDDKVKCKFIKKTGIKAEIEC
                     ETELTPDEAAGHCKSLFKKTPDGAVLYFSIQPDGFFG"
     gene            complement(2007..2267)
                     /locus_tag="PLK68_11265"
     CDS             complement(2007..2267)
                     /locus_tag="PLK68_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRM91023.1"
                     /translation="METIKVSSTSCPNNVAGAIASMIRNESKLQIQVIGAAALNQAIK
                     AIAIARGYIIPTGNEIVCIPSFHDLIVDDKEITALRLLLELR"
     gene            complement(2313..>2516)
                     /locus_tag="PLK68_11270"
     CDS             complement(2313..>2516)
                     /locus_tag="PLK68_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HRM91024.1"
                     /translation="ISDVGMTGPYMSAIGCDLDAIVTRLRGFGAPFIVAESSGQLSGV
                     IITFEENTPVAIERILINQDHPY"
BASE COUNT          772 a          496 c          310 g          896 t
ORIGIN      
        1 aattagctgc tcatactttt gatattcttt aatgacatca agattcaata aatatttatt
       61 taactgttta gcacaatcca aactacgatt caattgattc ccctttcaaa gcccatgttt
      121 tggcttcagt tatttttact ttaataattt ttccaatatc tttaggatca cctttaaagt
      181 ttaccaattt ttgatgccga gtatacccag tcaacattga atcatccttc ttagatgttc
      241 cttcaactaa tacttcgaca atcttattta agaaacgttg attttgctta tacgcttttt
      301 cattaacgat ttcattcaat ttatataatc gttggtcttt ttcatcactt gctacattat
      361 caaccatttt cgcggctggt gttcctgccc gaggtgagta gacaaatgta taagctaaat
      421 catattcaca ctcttgatat aaagacaatg tatctagata ttgttcatga gtttcatttg
      481 gaaaccccac gataatatct gtcgtgactg tgcagtcagg aatatattct ttgatcttat
      541 ggaataattc tagatattgt tcacgactat aacggcgccc catcaacttt aatacttcat
      601 tattccccga ttgaactggt aaatgaattg ccggcatgat attatcatat ttcgcaataa
      661 ttttgatcat atcttcacta aaatcccaag gatgactcgt tgtaaaacga attcgtggaa
      721 tatttgtttt agcgacatcc tcaagtaagt ttgagaaatt ataatcctca cctaaatctt
      781 taccataact gttaacattt tgcccaagaa gcgttatttc ttgatagcct tcagcaacca
      841 gttcttcaac ttctttgata atatcttttg ctaaacgaga acgttctttt ccacgtgtat
      901 aaggaacgat acaataagta caaaatttat tacatccata cnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnaactggt gcgttttcaa ttacatcgcc ctctttagac
     1021 caaacttcaa caatcatctc tttactataa agtgcatctt ttaataactc aggtaatcga
     1081 tgtatgttgt gagtaccaaa aatcaaatca acatggggat gcttttccaa tatccgatta
     1141 acaacaacct cttcttgagc catacatcca cacattgcga aaattagatt tggattagtc
     1201 ttctttaaat ttttgacata tccaactttt ccaaatacct tttcttcggc attttcacga
     1261 attgcacaag tatttaaaac aatcacgtca gcctctttaa tttctgtagt cggttgatat
     1321 gacatacttt ccaatattcc tgataaagtt tcactgtcac gttcatttgc ttgacaccca
     1381 tatgtttgaa tataatattt ttttcctagt ccaactttga ccatatctcc tggaacatga
     1441 taggcatcat catagcgacc aacttcgtca cgacttcttc ttttaatgaa ggccctttaa
     1501 aataattact ataatctttc atttattcac cacttttcta tcatattata tacataaatc
     1561 tctattattt ccacaagaaa aaattcattt ttattaaatc taattcttgc ttttatcgta
     1621 aataaaaaaa taaggactta cgtccttatt atccgaagaa tccatctggt tgaatagaga
     1681 agtataaaac tgctccatca ggtgtctttt taaaaagact cttacaatgt ccagctgctt
     1741 catctggtgt aagttctgtt tcacattcaa tttcagcctt gattccagtt tttttaatga
     1801 atttacattt tactttatca tcttcatatc catcaaaaaa atcgattaat ttttgtgttt
     1861 gtgcagtttg cattaataat ttacattttg ccataataaa tctcctccta tatcacgcct
     1921 actattataa cgcaaaacac taaaaaaaga aacccttttc atctaaagta gggttacttt
     1981 ttttgttaag tttttattac ttaaaattat cgtaattcta acaataacct aagtgctgtg
     2041 atttccttat catcaacaat tagatcatga aatgaaggaa tacagacaat ttcatttcca
     2101 gttgggataa tataaccccg agcaattgca attgccttaa ttgcttgatt taaagcagca
     2161 gctccaataa cctgaatctg taatttagat tcatttctaa tcatactagc aatagcgcca
     2221 gccacattat tagggcatga cgttgatgat actttaattg tttccataat tttcctccta
     2281 aaaaaatata tgaaaacatt taattattat acctaatatg gatgatcttg attaattaag
     2341 attcgttcaa tcgctacagg tgtattttcc tcaaaagtaa ttattactcc agataactga
     2401 ccactacttt cagctacgat aaatggcgct ccaaaacctc ttagccttgt gacgattgca
     2461 tcaagatcgc aaccaattgc gctcatatac ggccccgtca tccctacatc actgat
//
LOCUS       DAOMTA010000449         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1895_length_2508_cov_2.660008, whole genome shotgun sequence.
ACCESSION   DAOMTA010000449 DAOMTA010000000
VERSION     DAOMTA010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1895_length_2508_cov_2.660008"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="thrC"
                     /locus_tag="PLK68_11275"
     CDS             complement(<1..1196)
                     /gene="thrC"
                     /locus_tag="PLK68_11275"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRM91025.1"
                     /translation="MEKKYISTRNQQKEINFYQAIVQGIGEDGGLLVPDFDFTKMDLD
                     VLSKLNYVDLATEVLSTFVPEEGKELIHDACLNAYGKGLFPEIVVPVKKAGDVYVAEL
                     FHGQTAAFKDMALSLLPYLMTLSLKQLKEEREVMILAATSGDTGKAALEGFKDVEGTC
                     IKVFYPLDGVSAIQQQQMVSQTGKNVKVVGIHGNFDDAQSAVKKAFASEELKVASDQH
                     NVFLSSANSINVGRLIPQIVYYFHSYFELVRNNEIKLGDKINFCVPSGNFGNCLAGYF
                     AKKMGLPIDKFVCASNKNNILTDFFTTGKYDANREFYKTNAPAMDILVSSNLERLVWF
                     MSDGDGEKVRQYMDKLNSEGVYEVDDATFAKIKDQFKAGCLSEDEILTTIKTCFNETG
                     YLLDTHT"
     gene            1526..>2508
                     /locus_tag="PLK68_11280"
     CDS             1526..>2508
                     /locus_tag="PLK68_11280"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM91026.1"
                     /translation="MKIGVIGLGTVGYGVIEILTNERKRLEKVINDEVMIKYGCGLEE
                     VDLPEGVIYTKDFNDVINDHEIDVVVELIGGVTVAKTIILEAIRNGKHVVTANKALLA
                     HDGKEIFMAAKEKNVHIFYEASVGGGIPVLVSQKESLVANNTKEIIGILNGTTNFILT
                     LMENENLDFDEALTIADGLGYVEANPALDLDGIDAAHKICILAQNGFKKYIDFDKISA
                     SGIRNVTKVDMDYAKKLGYRFKMIAQAKEVNNGIGIDVAATLVSKKELVANVMDAYNV
                     VEIDNDYVENVIYYGKGAGRYVTASAVVSDIIKTTQVERWDHDYEDCSNIYP"
BASE COUNT          856 a          402 c          394 g          856 t
ORIGIN      
        1 gtatgcgtat ctaataaata tccagtttca ttgaaacatg ttttaattgt agttaagatt
       61 tcatcttcac ttaaacatcc tgctttaaat tgatctttaa ttttagcaaa agttgcatca
      121 tctacttcat aaacaccttc gctatttaat ttatccatat attgacgaac tttttctcca
      181 tcaccatcag acataaacca aaccaatctt tctaagttac tagatactaa aatatccata
      241 gctggagcat ttgttttata aaactcacga tttgcatcat atttaccagt agtaaagaaa
      301 tctgttaaaa tattattttt atttgaagca caaacaaatt tatcaattgg taaacccatt
      361 tttttagcaa aatatcctgc taaacagtta ccaaaattac cactaggaac acagaaatta
      421 atcttatctc ctaatttgat ttcgttattt cttactaatt caaaataact atggaaatag
      481 taaacgattt gaggaatcaa acgtccgaca ttgattgaat ttgcagatga taagaaaaca
      541 ttatgttgat cacttgcaac tttcaattct tcagatgcaa aagctttttt taccgcactt
      601 tgagcatcat caaaattacc gtgaattcct actaccttaa cattctttcc tgtttgactg
      661 accatctgtt gttgttgaat cgcagatacc ccatccaaag gatagaatac tttgatacaa
      721 gttccctcaa catctttaaa accttctaat gccgctttac cggtatcgcc actagtagct
      781 gctaagatca ttacttcacg ttcctctttt aattgtttta aagataacgt cattaaataa
      841 ggtagtaacg ataatgccat atccttgaat gctgccgttt gtccatggaa tagttcagca
      901 acataaacat caccagcctt ttttactggt acaacgattt ctggaaataa tccttttcca
      961 tacgcattta aacacgcatc atgaatcaat tcttttcctt cttcaggtac aaatgtactt
     1021 aatacttcag ttgctaaatc aacataattc aatttagata aaacatctaa atccatcttt
     1081 gtaaaatcaa aatccggtac taataaccca ccgtcttccc caattccttg gacgatagcc
     1141 tgataaaaat taatctcttt ttgttggttt cgtgtactta tatatttctt ttccattgtt
     1201 catccccctg tgtgatttaa actattgtat acaatttatt tatataagtc aatacatact
     1261 tattataaca tatcaaagta aaataacttt gattatttca attattattg attaccattg
     1321 actattgtat acaatcgtgg taatatgtca taaattagga aattggagga ccgagatatg
     1381 ttatcaatga acatgtttgc aataattatg gcaacgatta gttccattat tgtgcatgct
     1441 caataatata ttggttctgc tatttattta atgatattgg gaccttattt taaaggtcta
     1501 tttttattat ataaggggga attagatgaa aatcggtgtt atcggactag gtactgttgg
     1561 ttatggagtt atcgaaatcc taactaatga aagaaaacgt ctcgaaaaag tcattaatga
     1621 tgaagtaatg attaaatatg gttgtggtct tgaagaggtt gatctaccag agggggtaat
     1681 ttacacaaaa gactttaatg atgttataaa tgatcatgaa attgatgttg tagttgaatt
     1741 gattggtgga gtaactgtcg ctaaaacaat tattttagaa gctataagaa atggtaaaca
     1801 tgttgttact gccaataaag cattattagc ccatgatggt aaagaaatct ttatggcagc
     1861 aaaagaaaaa aatgtccaca ttttctacga agctagtgtt ggtggcggaa tccctgtttt
     1921 agtcagccaa aaagaaagtt tagtcgctaa caacacaaaa gaaatcattg gtatattaaa
     1981 tggaacaaca aactttattt taactttgat ggaaaatgaa aatttagatt ttgatgaagc
     2041 tttgaccatt gccgatggcc taggttatgt tgaagcaaat ccagcattgg atttagatgg
     2101 aatcgatgct gcccataaaa tttgtatttt agctcaaaat ggatttaaaa aatatattga
     2161 ttttgataaa attagtgcta gtggaatccg taatgtaact aaagttgata tggattatgc
     2221 taaaaaactg ggatatcgtt ttaagatgat tgctcaggca aaagaagtta ataacggaat
     2281 tggaattgat gtcgcagcta ctctagttag taaaaaagaa ctagtagcaa acgtcatgga
     2341 tgcatataat gtagtagaga tcgataatga ctatgttgaa aatgttattt actacggtaa
     2401 aggtgcaggt cgttatgtta ccgcttctgc agttgtaagt gatataatta aaactaccca
     2461 agtagaaaga tgggatcatg attatgaaga ctgctcaaac atctaccc
//
LOCUS       DAOMTA010000450         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1897_length_2507_cov_2.464519, whole genome shotgun sequence.
ACCESSION   DAOMTA010000450 DAOMTA010000000
VERSION     DAOMTA010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_2507_cov_2.464519"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLK68_11285"
     CDS             <1..1153
                     /locus_tag="PLK68_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM91027.1"
                     /translation="GTSNNNIFTSFGLDYKYLDDGHDIEKLIELFESVKNIDHPIVLH
                     IHTIKGKGLPYAEVNKEAWHAGGPFNVADGTYKFVDEDDQTLFNSLTNLLNANIVVNA
                     GTPMGLGFIEPVRKEYVERGQFIDVGIAEENAMAMISGIAKNGGIPVFGTFAPFLQRT
                     YDQLSHDLCLNNNPATILVLSAGVYGMNSNTHIALCDIAMFAHIPNLIYLAPTTKEEY
                     LQMFKYATTQKDHPIAIRVPVQMIESGQEDTTDYSIHNKSQIVQQGNDIAIIGVSNLL
                     PLALKTAQKYKEDTGKNITVINPKFLTGLDEELLNDLTNNHRLIITLEDGELEGGYGH
                     RISSFYGMTEMKVKNYGISKAFHTDFNADELLAQHGISVDQLVTYMKNS"
     gene            complement(1563..1826)
                     /locus_tag="PLK68_11290"
     CDS             complement(1563..1826)
                     /locus_tag="PLK68_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91028.1"
                     /translation="MDDICKKYGICPRLKLQLWIKKYNNHEELKSSEIGEHTIMTKGR
                     KTAFGGCTEKALKTNKKTDGLLMRLKSSIQSHQIEDNEELEMI"
     gene            complement(2182..>2507)
                     /locus_tag="PLK68_11295"
     CDS             complement(2182..>2507)
                     /locus_tag="PLK68_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM91029.1"
                     /translation="KTYRGKSAISDQLVFVTVYGDAYCRSGFNSTLNYSINKTLKSGY
                     EFECPRLSPHIFRHTFATRCLEAGMSPNTVSSLLGHGTIRMTLSYVHNSSEKFKEDTS
                     LLNRI"
BASE COUNT          843 a          419 c          382 g          863 t
ORIGIN      
        1 cggaacaagt aataacaata tatttacatc atttggtctt gattataaat acttagatga
       61 tgggcatgat atcgaaaaat taattgaatt atttgaaagt gttaaaaata tcgatcaccc
      121 tatcgtattg catatccata caatcaaggg aaaaggatta ccatatgcag aggtaaacaa
      181 ggaagcttgg catgctggtg gtccatttaa tgttgctgat ggaacatata aatttgttga
      241 tgaagatgat caaaccctat tcaatagctt gactaactta ttaaatgcaa atattgttgt
      301 aaatgccggt acaccgatgg ggctaggatt catcgaacct gtaagaaaag aatatgtcga
      361 acgtggacaa tttattgatg ttggcattgc tgaagaaaat gctatggcaa tgattagcgg
      421 gattgctaaa aatggcggaa tacctgtctt tggaactttt gctccttttt tacaacgtac
      481 atatgatcag ctatctcacg atttatgtct gaacaataat ccagcaacaa ttcttgttct
      541 atccgctgga gtctatggga tgaattcaaa tactcatata gctttatgtg atattgcaat
      601 gtttgcacat attccaaatt taatctattt agcgccgaca actaaagaag agtatttaca
      661 aatgtttaaa tatgcaacta cccaaaaaga tcatccaatt gcaatcagag tcccagtaca
      721 aatgattgaa agtggtcaag aagacacaac tgattactct atccataata aatctcaaat
      781 cgttcaacaa ggtaatgata ttgctattat cggagtaagt aatctgcttc cattagcctt
      841 aaaaacagct caaaaataca aagaggatac cggtaaaaat atcactgtaa taaatccaaa
      901 atttctaaca ggtttagatg aagaattact aaatgacttg actaataatc atcgtcttat
      961 tattacttta gaagatggtg aattagaagg tggatatggg cacagaataa gcagtttcta
     1021 cggtatgaca gagatgaaag tcaagaatta tggtatctcc aaggcctttc atactgattt
     1081 taatgctgat gaattattag ctcaacatgg tatatccgtt gatcaattag tgacttatat
     1141 gaaaaatagc taaaaactat atattattgt tttatatata attctcatta gtatacaata
     1201 aaattaataa tgtatttaaa aaaggctgta acgtctaaag tgaactgcaa cctgtcaagt
     1261 agacaattga aaaaatataa aatttttcct gccggtagat tatctactgg cagtttttat
     1321 gcgatttgca aataaatttt atgtttttcg attctgatat aataatggaa ttctgttaca
     1381 tctgtgagcc gtttctcatt tagtgcatcg gtagtaaact ctcgattaag taaattttta
     1441 gctatatatt gaggatttac agcctgtcct gtacatccat tagtagagta tttaatagtt
     1501 gatttgatat taagtagtac gtagtactcg tttatcatta acatcaatgt catgataaga
     1561 ttctagatca tctctaattc ttcattatcc tctatctggt gactctgtat ggagcttttc
     1621 aatctcatca gtaatccgtc tgtttttttg ttcgttttca gagctttctc tgtacaacct
     1681 ccaaatgcag tttttcttcc ttttgtcata atagtgtgtt ctcctatttc agatgatttt
     1741 aactcttcat gattattata cttctttatc cagagttgta acttcaatct tggacaaatt
     1801 ccgtattttt tacaaatatc atccaaggaa ccacgaccat ctaaataatc taaaacagct
     1861 tgctgacacc aaacatagat acaattcgat tttggctttc ttttccatct agatacaaat
     1921 ttacagcata taccttatcc tcaatggata tttttctttt agacataaaa tatgcttcct
     1981 cctaagtgac aagtttttat tatttcactt gtctccccag aaagaagcat atcaatttta
     2041 atgcgttaca gtctctttta tttataacta tttaacaaca atattgacaa gaatgtggtg
     2101 aatcatcaaa aaagcgccca taaaatcaat gctttttaag attaaatttt ctcaatacca
     2161 actcaatgcc agcaaaaaca gttaaattct attcaataaa gacgtatctt ctttgaattt
     2221 ttctgaacta ttatgcacat aagaaagtgt cattctaatt gttccatgtc ctaacaatga
     2281 acttactgta tttggtgaca tacctgcctc caaacatctt gttgcaaaag tatgtctgaa
     2341 tatgtgtgga gataatcttg gacattcaaa ctcataccct gattttaaag ttttatttat
     2401 cgaatagtta agagtactat taaatccaga tcggcaatat gcatcaccat aaactgtaac
     2461 aaatactaat tgatcactta tggcactctt acctctatat gtttttc
//
LOCUS       DAOMTA010000451         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1898_length_2507_cov_2.282219, whole genome shotgun sequence.
ACCESSION   DAOMTA010000451 DAOMTA010000000
VERSION     DAOMTA010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1898_length_2507_cov_2.282219"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLK68_11300"
     CDS             complement(<1..495)
                     /locus_tag="PLK68_11300"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRM91030.1"
                     /translation="MGKIGFVYAGQGSQVVGMGQSFYDNYQIAKDVFDNIDLDIDVKK
                     LCFEGPLEELSLTRNTQPCMVAVAVVATKLLKENGIAPDYVAGLSLGEYSALNAAGVL
                     DDQTAINLVRFRGQAMERAAAGIESKMIAIIGLDRELLNEAVKEARALGVVSIANYNC
                     PGQLV"
     gene            complement(495..1559)
                     /locus_tag="PLK68_11305"
     CDS             complement(495..1559)
                     /locus_tag="PLK68_11305"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRM91031.1"
                     /translation="MKKVNIGDMTLDVPIIQGGMGVGISLGNLAGNVALNGGMGVIST
                     AHPGYRADDFEKNPLEANKRELANEIKKAKEIAKGKGMVAINAMVAITDYAALVEVAV
                     KSKIDAIISGAGLPMNLPSFVQGTKVKIAPIVSSGKAAKLICKTWDRKFKVAPDFVVI
                     EGSEAGGHLGFHKEDVLNKTTAKLVDIFKEVKETVQPFVEKYQKDIPIFVAGGVYDSE
                     DIQKYLDLGADGVQMATRFICTEECDADIKYKQAFINAKKGDIEIVKSPVGMPGRAIM
                     TKLTERLKQDERIPVKRCYNCLIPCDVKTTPYCISGALINAAKGNLDEGLVFSGSNGY
                     RNDKIISVKELMDELKRGLK"
     gene            complement(1562..2494)
                     /locus_tag="PLK68_11310"
     CDS             complement(1562..2494)
                     /locus_tag="PLK68_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HRM91032.1"
                     /translation="MKLNKLLNIEYPLIQGGMANIATGEFAASVSNAGALGLIGAGGM
                     DTATLKKNIEICRNLTDKPFGVNIMLINPCADEMAQLVIDEKVPVVTTGAGNPGKYVA
                     AWKAAGIKVLPVVPSVALAKRLEKYNVDAIIVEGTEAGGHIGELTTMALVPQVVEAVS
                     VPVIAAGGIASGKQVLAAYALGACGVQVGTCLLASEECPIHENYKQAVIKAKDTSTTV
                     TGRIAGTPVRVIKNKMAKEYVKREKEGADMMELEKYTLGSLRRAVLEGDADTGSLMAG
                     QVVGMINEIRPLKVIIKELFDDCDKAFKKLESEF"
BASE COUNT          747 a          581 c          313 g          866 t
ORIGIN      
        1 tactaattgt cctggacaat tatagttagc aattgaaaca actcccaaag ccctagcttc
       61 tttaactgct tcatttaata attcgcgatc taagccaata attgcaatca ttttgctttc
      121 aattcctgct gccgctcttt ccatcgcttg accacggaaa cgtactaaat taattgctgt
      181 ttgatcatct aaaacacctg ctgcatttaa agctgaatat tcaccaagac ttaatcctgc
      241 tacataatct ggagcaattc cattttcttt taatagctta gttgctacta cagcaacagc
      301 aaccatacat ggttgtgtat ttcttgttaa agaaagttct tcaaggggac cttcaaagca
      361 taatttcttt acatcaatat ctaagtcaat gttatcaaaa acatctttag caatttgata
      421 gttatcatag aatgattgac ccattccaac tacctgacta ccttgaccag catatacaaa
      481 tccaatctta cccattattt tagtcctctc tttaattcat ccattaattc ttttactgaa
      541 ataattttat cattacgata tccattgctt ccactaaata ctaatccctc atccaaatta
      601 cctttagcag cattgatcaa tgcaccacta atacaataag gcgtagtttt aacatcacag
      661 ggaattaagc aattatagca gcgttttact ggaatccttt catcttgttt aagtctttct
      721 gttaacttgg tcatgatggc tcttcccggc attccaactg gtgatttaac aatctcgata
      781 tctccttttt tagcatttat aaatgcttgt ttatatttaa tatctgcatc acattcctca
      841 gtacaaataa accgtgttgc catttgaaca ccatcagcac ctaaatctaa atacttttga
      901 atatcctcac tatcataaac accaccagct acaaaaatag gaatatcctt ttgatatttt
      961 tcaacaaatg gttgaacagt ctcttttact tctttaaaaa tatctactaa tttagccgtt
     1021 gttttattta acacatcttc tttatgaaag cctagatgac caccagcttc acttccttca
     1081 atgacaacaa agtctggagc taccttaaat ttacgatccc aagttttaca aatcaattta
     1141 gcggctttac cacttgatac aattggggca attttaactt ttgttccttg aacaaaactt
     1201 ggtaagttca taggtaatcc agcacccgaa ataattgcat ctattttact ttttacagct
     1261 acttcaacta aagctgcata gtcagtaatt gcgaccattg cattaattgc aaccatccct
     1321 ttacctttag caatctcttt agctttcttt atttcattag ctagctcacg tttattagct
     1381 tctagcggat ttttttcaaa atcatcagca cgataaccag gatgagcagt tgaaataaca
     1441 cccataccac catttaaagc aacatttcca gccaaatttc caagtgaaat accaactccc
     1501 atgccacctt gtatgattgg tacatcaaga gtcatatctc cgatatttac ctttttcata
     1561 attaaaactc gctttctaat ttcttaaatg ccttatcaca atcatcaaat aattctttaa
     1621 taattacttt tagaggtcta atttcattga tcattccgac tacttggcca gccattaatg
     1681 aaccggtatc ggcgtcacct tctaaaactg ctctacgtag tgaacctaat gtatattttt
     1741 caagttccat catgtcagca ccttcttttt cacgcttaac atactcttta gccattttat
     1801 tttttattac ccgtactggt gttcctgcga ttcttccagt tactgtagtg cttgtatctt
     1861 tagctttgat tacagcttgt ttataatttt catgaatcgg acactcttcg cttgcaagca
     1921 gacaagttcc tacctgtact ccacaagccc ctagagcata cgctgccaat acctgttttc
     1981 cactcgcaat tccaccagct gctataacag gaacactaac tgcttcaact acttgaggta
     2041 ctaatgccat tgtagttaat tcacctatat gaccacctgc ctcagttcct tcaacaataa
     2101 ttgcatcaac gttatatttt tcaaggcgct ttgctaaagc tacacttgga acaactggca
     2161 atactttaat tcctgctgct ttccatgcag caacatattt accaggattt cctgctccag
     2221 ttgttacaac cggcactttt tcatcaatta ctaattgtgc catctcatca gcacaaggat
     2281 taatcaacat gatattgaca ccaaaaggtt tatcagttaa attacggcaa atttcaatat
     2341 tcttttttaa tgtagcagtg tccattccac cggcaccaat taaaccaagt gcgccagcat
     2401 tagatacact agctgcaaat tcaccggtag cgatatttgc cattcctcct tgaatcaaag
     2461 gatattcaat atttaataat ttatttaatt tcatcttcta actccat
//
LOCUS       DAOMTA010000452         2506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1900_length_2506_cov_2.616483, whole genome shotgun sequence.
ACCESSION   DAOMTA010000452 DAOMTA010000000
VERSION     DAOMTA010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_2506_cov_2.616483"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..538)
                     /gene="rpsI"
                     /locus_tag="PLK68_11315"
     CDS             complement(146..538)
                     /gene="rpsI"
                     /locus_tag="PLK68_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRM91033.1"
                     /translation="MANVQYRGTGRRKSSVARVILTPGTGNIVINDKPAKEYLPSDVL
                     LMIVNQPLELTNTTSQFDVSVNVYGGGYSGQAGAIRHGISRALLQAGTDYRPTLKAAG
                     FLTRDARVKERKKYGLKKARRAPQFSKR"
     gene            complement(556..993)
                     /gene="rplM"
                     /locus_tag="PLK68_11320"
     CDS             complement(556..993)
                     /gene="rplM"
                     /locus_tag="PLK68_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRM91034.1"
                     /translation="MRQTTMANAATIERKWYVVDATDLTLGRLASEVATVLRGKNKPT
                     YTPHVDTGDYVIIVNADKVVMTGKKLDTVNYYRHSGWLGGLKVKTARQFKEQNPTGWV
                     EAAVKGMLPHNTLGRKQGMKLFVYAGSEHPHAAQKPEELKIKG"
     gene            complement(1260..>2506)
                     /locus_tag="PLK68_11325"
     CDS             complement(1260..>2506)
                     /locus_tag="PLK68_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM91035.1"
                     /translation="CFNRPQYIQLNLAMGGNWPGDVETNLAGTEFAIDYVYYAQNEEQ
                     KAAAKEYYDAAPEISGLKDVTMQEGATPDLLAGVTSNRNSFVDFSIENEHMFKNEGGL
                     TSVDLLCTGKDDLASLAKLPVGKYNIHYTAIPNDIEYDGNRPERESDYKFTRKTMTLT
                     VAERTFPSDFKLNGIVGDKLANVALPEGWSWVDPETKITGTADEYDVKYVNGEYSKTV
                     KVVVNAVVVDKEGLKARLAEATAEAAKIDVYKAATIEKLNTVIEEATKVLNDTSANKE
                     AVEEAIRALNTAIANLEKYVTEAELDQVIAKGEEFLGKTDIYTKESLDILSTALAKVR
                     SAITSGDKEAIESAYAQLTEAIDKLVKIDAPTPEVKPEVKPENKPNTSVKTGDNTYLG
                     AIMTSLLLSAGGLVLFKKRKYN"
BASE COUNT          729 a          481 c          322 g          974 t
ORIGIN      
        1 tctattgctt cgataacaat attataccca tatcaacttc tccaagcgat taaattgtta
       61 ttaatcctac ttctgtttag ttacttataa aaagaaaaaa atccttgaat aatcaaggat
      121 tttataaaca acacatttga taaaattaac gttttgagaa ttgtggagca cgacgagctt
      181 ttttaagacc atatttttta cgttctttaa cgcgtgcatc acgagtaagg aatccagctg
      241 cttttaaagt tggtctataa tcagtaccag cttgtaataa tgctcttgag ataccatgac
      301 ggatagctcc agcttgtcca gagtatccac caccatatac gtttacacta acatcaaatt
      361 gagaagttgt gttagtcaat tctaatggtt gattaacaat cattaataaa acgtctgatg
      421 gtaagtattc tttagctggt ttatcgttaa ttacgatatt tcctgtacct ggtgttagga
      481 taacacgagc tacagaagat tttctacgtc cagttcctct atattgtaca tttgccattg
      541 ttctgtattt cccccttacc ctttaatctt caattcttct ggtttttgtg ctgcatgtgg
      601 atgttcacta cctgcatata caaataattt catcccttgt tttctaccta atgtgttatg
      661 tggtaacata ccttttaccg ctgcttcaac ccaaccagtt ggattttgtt ctttgaattg
      721 gcgagcagtt ttaactttta aaccacctaa ccatccagag tgacgatagt aatttactgt
      781 atctaatttc tttccagtca ttactacttt atcagcgttt acgatgatta cataatcacc
      841 agtatccaca tgtggtgtgt aagtaggttt atttttacct cttaacactg ttgctacttc
      901 tgatgccaaa cgacctaaag ttaagtcagt tgcatcaact acgtaccatt ttctttcgat
      961 tgtggctgca ttagccatag ttgtttgtct cattttactt tctcctatat ccttaccggg
     1021 gacttagagt tcataagaac attctcatta taaatacttc tatatttaat gtcaatgatt
     1081 tttctacaaa taaattacct tattatcata tatttttcta tttttgactt ttaaaaaaac
     1141 tctagaataa aaacaccatt ttaatcatta tttttattca ttaattactg ttactattta
     1201 atatttaact catttaaaaa gacagtgcta gaaataatca atagcactat ctttctattt
     1261 taattatatt ttcttttttt aaacaatacc aaaccgcccg cagataataa caatgatgtc
     1321 ataattgcac ctaaatatgt attatcacca gttttaacac ttgtattagg tttattttct
     1381 ggttttacct caggtttcac ttcaggtgta ggagcatcaa ttttaactaa tttatcaatt
     1441 gcttctgtta attgagcata agctgattca attgcttctt tgtcacctga tgttatcgca
     1501 cttcttactt ttgctaaagc agtacttaat atatctagtg actctttagt ataaatatct
     1561 gttttcccta aaaattcttc acctttagca atcacttgat ctaattctgc ttctgttaca
     1621 tatttttcaa gattagctat agctgtattt aaagctctaa ttgcttcttc tacagcctct
     1681 ttattagcac ttgtatcatt taatacttta gtcgcttctt cgattactgt atttaatttt
     1741 tcaatagttg cagctttata tacatctatc ttagctgctt cagctgttgc ttctgctagc
     1801 ctagctttta aaccttcttt atcaacgaca actgcattaa ctacaacttt tactgttttt
     1861 gaatattcac cattgacata ttttacatca tattcatctg ctgtacctgt aatttttgtt
     1921 tctggatcaa cccaagacca tccttctggt aatgctacat ttgctaattt atcaccaacg
     1981 attccattta atttaaaatc actagggaaa gtacgttcag caactgttaa agtcatcgtt
     2041 ttacgagtga atttataatc actttcacgt tctggtctat ttccatcata ttctatatca
     2101 tttggaatag cagtataatg aatgttatat ttaccaactg gtaattttgc taaactagct
     2161 agatcatctt tgcctgtaca aagtaaatca acactagtta atccaccttc atttttaaac
     2221 atatgttcat tttcaattga aaaatctaca aaactattac gattagatgt aacaccagct
     2281 aatagatcag gtgttgctcc ttcttgcatt gttacatctt ttaacccact aatttctggt
     2341 gcagcatcgt aatattcttt tgctgcagct ttttgttctt cattttgagc ataatataca
     2401 taatcaattg caaattcagt tcctgctaaa ttagtttcta catcaccagg ccagtttcca
     2461 cccatagcta agtttaattg aatatattgt ggacgattaa aacatt
//
LOCUS       DAOMTA010000453         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1906_length_2501_cov_2.684791, whole genome shotgun sequence.
ACCESSION   DAOMTA010000453 DAOMTA010000000
VERSION     DAOMTA010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1906_length_2501_cov_2.684791"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>2501
                     /locus_tag="PLK68_11330"
     CDS             99..>2501
                     /locus_tag="PLK68_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM91036.1"
                     /translation="MKGTQADLNWLADPKVFAVNRLNAYSDHSYYLDIDKALNGDAME
                     LKQSLNGRWYFNYAKNPNERFVDFYKEDIDCHYFDMIDVPGHIQMQGYDQMQYINTLY
                     PWDGHEKLRPPHISSDDNPVGSYVCYFEVNEALKNKTTRLVFDGVETAYYVWLNGEFI
                     GYSEDSFTPSSFDVTYALKDGENKLAVEVYKRSSASWLEDQDFWRFSGIFRDVSLYAI
                     EDIHINDLFVKTTLKNNYKDVQVSVNLDVIAKKEGYLNTALYDQNNNIIYQAKQLPLT
                     NFSFGLTNVNLWSSENPYLYKLLLTVYDHDDNLVEVIPQKIGFREFKMDQGIMKLNGQ
                     RIVFRGVNRHEFAADKGRAITKEDMLYDIKFMKMHNINAVRTSHYPNQSLWYDLCDEY
                     GIYLIDEANLESHGSWQKLGACEPSWNIPGNLPQWHDVVVDRANTMLQRDKNHPSILI
                     WSCGNESYAGTNIVAMANHFRENDPTRLVHYEGCVWNRDYCEATDMESRMYAKAKEIE
                     TYLQGNPAKPYISCEYMHAMGNSLGGMKHYTDLEDKYAQYQGGFIWDYIDQAVYYTNG
                     YGEKVLGYGGDFKERYTDYNFCGDGIVFADRTISPKAQEVKYLYQDIIIKPTDAGVII
                     TNKMMFSNTSKYQFVYQLKQGKKVLQEGCFIADVKPGDSKEILIPWLTKLDKEAVKTV
                     SALLKDDQIWAKAGFEVAFGQTIVGEYKPLSVSKQPLKVIIGDGNIGIRYNDFEVMFA
                     NSEGLISLKYGNHEYIARAPRPVFSRASTDNERGYKHEVNSSMWFGASTFYNCTKFNY
                     EVA"
BASE COUNT          867 a          291 c          497 g          846 t
ORIGIN      
        1 tataaataat tatcttatta aaagaattga aaaatttatt atattttagt aaaatatata
       61 tgtatttcac taaaatataa gtaaaattgg aggctattat gaaaggaaca caagcagatt
      121 taaattggtt agcagatcct aaagtttttg cggtaaatcg cttaaatgct tatagtgatc
      181 attcttatta tctcgatatt gataaagcat tgaatggaga tgcaatggaa ttaaagcagt
      241 ctttaaatgg acgctggtat tttaattatg ctaaaaatcc taatgaaaga tttgttgatt
      301 tttataaaga ggatattgat tgtcattatt ttgatatgat cgatgtacca ggtcatattc
      361 aaatgcaagg ttatgatcag atgcagtata ttaatacttt atacccatgg gatgggcatg
      421 agaaattaag accaccacat atatcaagtg acgataatcc tgtaggaagt tacgtttgct
      481 attttgaagt aaatgaagca ttaaagaata aaacaactcg tctggttttt gatggagttg
      541 aaacggctta ttatgtttgg ttaaatggag aatttattgg ttatagtgag gattcattta
      601 cgccatcaag ttttgatgta acttatgctt taaaagatgg tgaaaataaa ttagcggttg
      661 aggtttataa acgctctagt gccagttggt tagaagatca ggatttttgg cgcttttcag
      721 ggatttttag agatgtaagt ttatatgcta tagaagacat tcatattaat gatctgtttg
      781 ttaagactac tttaaaaaat aattataaag atgtccaggt atcggtcaat ttagatgtaa
      841 ttgctaaaaa agagggctat ctaaatactg ctttatatga tcagaataat aatattattt
      901 atcaagcaaa gcaattgcca ttaactaatt tttcgtttgg actgactaat gttaatttat
      961 ggtcaagtga aaatccatat ttgtataaac tattgttaac cgtttatgat catgatgata
     1021 atttagtaga agttatacca caaaaaattg gatttagaga gtttaaaatg gatcaaggga
     1081 taatgaagtt aaatggtcaa agaattgtct ttagaggtgt taatcgtcat gaatttgctg
     1141 ctgataaagg tcgggctatt acaaaagagg atatgctata cgatatcaaa tttatgaaaa
     1201 tgcataacat taatgcagta agaacatctc attatccaaa tcaatcattg tggtatgatc
     1261 tatgtgatga gtatggaatt tatttaattg atgaagcaaa tttagagagt catggttcat
     1321 ggcaaaaatt aggtgcttgt gaaccgtcat ggaatattcc aggaaacctg ccacaatggc
     1381 atgatgtcgt tgttgatcgt gctaatacaa tgctacaacg tgataaaaat catccttcga
     1441 ttttaatttg gtcatgtggt aatgaatcat acgcgggaac aaatattgtt gctatggcta
     1501 atcattttag agaaaatgat ccgactcgtt tagttcatta tgaaggttgt gtatggaatc
     1561 gtgattattg tgaagcaact gacatggaat cacgtatgta tgcaaaagcg aaagaaattg
     1621 aaacatattt acagggcaat cctgcaaaac cgtatattag ttgtgaatat atgcatgcta
     1681 tgggaaactc tctaggtgga atgaaacatt atactgattt agaagataag tatgctcaat
     1741 atcaaggtgg ttttatttgg gactatattg atcaggcagt gtactataca aatggttatg
     1801 gtgaaaaagt tcttggttat ggtggagatt ttaaagaacg ctatactgat tataatttct
     1861 gtggcgatgg aattgttttt gctgatcgta ctatttcgcc aaaagctcaa gaagttaaat
     1921 atttatatca agatattatt attaagccga cagatgcagg tgtcataatt acaaataaaa
     1981 tgatgtttag taatactagt aaatatcaat ttgtttatca attaaaacaa ggtaaaaaag
     2041 ttttacagga gggatgtttc atagccgatg taaaacctgg agactctaaa gaaatattga
     2101 ttccgtggtt aacaaaatta gataaagaag ctgttaagac agttagtgct ttattgaaag
     2161 atgatcaaat ttgggctaaa gctggttttg aagttgcttt cggtcaaact atagttggtg
     2221 aatataaacc gctctcagtt tctaaacaac cgctaaaagt tattattggt gatggtaata
     2281 tcggtataag atacaatgat tttgaagtga tgtttgctaa tagtgaagga ttaatttcat
     2341 taaaatatgg gaaccatgaa tatatcgcaa gagctccacg tcctgttttt agccgagcat
     2401 caactgataa tgaacgcggg tataaacatg aggtgaatag ttcaatgtgg tttggggctt
     2461 caacttttta taattgtact aagtttaatt atgaagttgc c
//
LOCUS       DAOMTA010000454         2494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1913_length_2494_cov_1.835178, whole genome shotgun sequence.
ACCESSION   DAOMTA010000454 DAOMTA010000000
VERSION     DAOMTA010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_2494_cov_1.835178"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLK68_11335"
     CDS             <1..841
                     /locus_tag="PLK68_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HRM91037.1"
                     /translation="KAIVTVNDINNNYGEEYKYSYSLEGVVARDIDTLVSSIVCVTDN
                     NENVGNHKIKAIINEDVAKNYKEVVVVKGKHTITPKAININIDNINTTYGDDEKVLTF
                     SLAVDSTLAYGDTLADLGITLERESGNDVGEYKITGDASNENYDVTFTDGTYTIASKK
                     VQLIIDDKMKVEGEIDPEFTYNIIGLVNGDIFTVELTREPGEKAGKYVINGKVEGANN
                     YNLEIVTGTLTVNAKTALPVKPVDKGTPTGDNVDLNTLIALAGISALLLLIILRKIHS
                     LNK"
     gene            1101..1598
                     /locus_tag="PLK68_11340"
     CDS             1101..1598
                     /locus_tag="PLK68_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91038.1"
                     /translation="MSLVKNMDNWSDMSKLDLLYKFSIIKYLRYFIIPELFGLSILVI
                     ASIKIRGIFIFIIIVVIMMLYQINKISQSFIKEKAGKLIYRQKTSFFRSLRNFDEYTY
                     SDYCIRKIKKVKSFFNYYVINGEIVKRSFIAYMDDDEICIDEKYCTVLKIPKAYHGLD
                     KLRDF"
     gene            complement(1652..>2494)
                     /locus_tag="PLK68_11345"
     CDS             complement(1652..>2494)
                     /locus_tag="PLK68_11345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM91039.1"
                     /translation="VGESLSLKDMFKSIAIASANDASVAVAEHIAGSINKFVEMMNDK
                     AKKLGLKNTHFKNATGLHDPDHYTCAHDLAIMAAYLIEIGGEDLFAVTSLYDSYIRED
                     TKQSFWLVNTNKLLKLYNGVDGLKTGYTKEAGYCLVTTAQRDGQRIIGVLMKETAPKT
                     RNEEMCSLLDYGFNNYKQVTIFKKDTLIEQHLVDKMDNLTIDVKCKKDIVYTKAKSAD
                     AKVSTKVNYKENFLPVKQGDVIGTLDVIVDGKTIATYDVYSDTNASKSTYLSKTIKTL
                     KYLF"
BASE COUNT          932 a          312 c          391 g          859 t
ORIGIN      
        1 taaagcaatt gtaactgtta atgatataaa taataattat ggtgaagaat acaaatattc
       61 atattcttta gagggtgtag tagcacgcga tattgataca ttagtatcat cgattgtttg
      121 tgttacagat aacaatgaaa atgtagggaa tcataaaata aaggcaatta tcaatgaaga
      181 tgtagctaaa aactacaaag aagtagtagt tgtaaaagga aagcatacaa ttacacctaa
      241 agctataaat attaatattg ataacattaa tacgacatat ggtgatgatg aaaaagtatt
      301 aacattttca ctagcagtgg atagtacttt agcttatggt gatacattgg cggatttagg
      361 aatcacttta gaacgtgaat cgggaaatga tgtaggtgaa tataagatta ctggtgatgc
      421 aagtaatgaa aactatgatg taacatttac ggatggtaca tatactatag ctagtaaaaa
      481 agtgcagcta ataatcgatg ataaaatgaa ggttgaagga gaaattgatc cagaatttac
      541 ttataatatt ataggattgg ttaatggaga tatatttact gttgagttaa ctagagaacc
      601 aggtgaaaaa gcaggaaaat atgtaatcaa tggtaaagtc gaaggggcca ataattataa
      661 tttagaaatt gttactggta ctttaacggt caacgctaaa accgccctac ctgttaaacc
      721 tgtagataag ggaaccccaa ccggtgataa cgtagattta aatactttga tagcattggc
      781 aggaattagt gccttactat tgttaattat tttaagaaaa atacatagtt taaataaata
      841 gctattatat gaaattcccc actaaaaaat gatatgatcc ctctaaagta gacaatgtaa
      901 ataaaacatt gtttacgaaa ggagggatca aaatagtggg gaatattatt ttattattga
      961 acttatggat atgtttataa gtataatact aaaataatta aattataaat catataaaaa
     1021 cttaatatca actttactaa aagtgacttt taatcctctt tgataaagat ataatggaat
     1081 aaaaataagg agtgataaca atgtcattgg taaagaatat ggataattgg agtgatatgt
     1141 ctaaattaga tttattatac aaattttcaa taataaagta tcttcggtat ttcattattc
     1201 cagaattgtt tggcttaagc atcttggtaa ttgcgtcaat aaaaataaga ggaatattta
     1261 tatttataat tattgtagtt ataatgatgt tgtatcaaat taataaaata agtcagtctt
     1321 ttatcaaaga aaaagcaggt aaattgattt atcgtcaaaa aacaagtttt tttagatcat
     1381 taagaaattt cgatgaatat acatatagtg attattgcat tagaaaaata aaaaaagtta
     1441 aatcgttttt taattattat gttattaacg gggaaattgt taaacgtagt tttattgctt
     1501 atatggatga tgatgaaatt tgtatagatg aaaaatattg tacagtatta aaaattccta
     1561 aagcatatca cggtttagat aaattaagag atttttaaga aataggctaa ggcctgtttt
     1621 tgtgtatata aaaaacggat tttaatccgt tttaaaataa atattttaat gttttaattg
     1681 tttttgatag atatgttgat ttagaagcat tagtgtcact ataaacatca taggtagcaa
     1741 ttgttttgcc atcaacaatg acatctaatg tgccaataac atcaccttgt ttaaccggta
     1801 agaaattttc tttatagttt actttagtag aaactttagc atcagcactt tttgctttag
     1861 tatatactat atctttctta catttgacat caatagtcag gttatccatt ttatcaacta
     1921 gatgctgttc aattaaagta tcttttttaa aaatggtaac ttgcttataa ttattaaaac
     1981 cataatccag taaactacac atttcttcat ttcttgtttt tggagctgtt tctttcatta
     2041 ggacaccaat aattctttga ccatcacgct gtgcagtggt aactaagcaa taaccagctt
     2101 cttttgtata tccagttttt agaccatcaa caccattata taactttaac aatttattag
     2161 tattgactag ccaaaagctt tgtttcgtat cttcacgaat ataactatca tacaatgacg
     2221 ttacagcaaa taagtcttca ccgccgattt caattagata ggcagccatt attgctaaat
     2281 catgagcaca agtgtagtga tcgggatcat gtagaccggt tgcattttta aaatgagtat
     2341 tctttaaacc taatttttta gccttatcat tcatcatctc tacaaactta ttaatacttc
     2401 cagcaatatg ttcagctact gccacactag catcattagc actagcgata gcaattgatt
     2461 taaacatatc ttttaaagat aaactttcac caac
//
LOCUS       DAOMTA010000455         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1914_length_2493_cov_1.884742, whole genome shotgun sequence.
ACCESSION   DAOMTA010000455 DAOMTA010000000
VERSION     DAOMTA010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_2493_cov_1.884742"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLK68_11350"
     CDS             complement(<1..311)
                     /locus_tag="PLK68_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRM91040.1"
                     /translation="MRRKIITLILIIGLWQGFALSIDKAVILPLPLVVFNQMFNLATS
                     QSFYIAIGATLSRVALSFFLALIVGTILGVFSGLFKSVNEYLAPIFSFLQTIPQIAYI
                     L"
     gene            complement(308..1201)
                     /locus_tag="PLK68_11355"
     CDS             complement(308..1201)
                     /locus_tag="PLK68_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91041.1"
                     /translation="MKKIIKYLLCLMMIFTMIGCSNTETKDKSNTVKILCPAGAPSLA
                     FVSEYENISKKGKIDFVDGSDQLVAELSKNSSDYDIIVAPINLGAKLIASEQTDYRIQ
                     GVITWGNLYYIGTSNEALNQTGELGLFGEGAVPQKIVDTVKINTSLTPKYYQSATLVQ
                     QQLLSGNIQVGMLAEPLASATIAKAKQAGIELSIIADLQKEYGKDGYPQAAIFVKENN
                     SYDELFEAIDQFTNNDYNGLKDYLEKIGIETLSLPSVEITVKSIDRQNVHYKKASECS
                     EQIKDFLKLFNIDYNKNMLSS"
     gene            complement(1222..>2493)
                     /locus_tag="PLK68_11360"
     CDS             complement(1222..>2493)
                     /locus_tag="PLK68_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HRM91042.1"
                     /translation="KTQGLGILGFMNRGFKTKVGPVYDKSMNDVNCMQCGQCINVCPV
                     GALQEKEEVHNVIAALNDDSKHVVVQTAPAVRASLGEEFGMPIGTRVTGKMVHALKLM
                     GFDRVYDTNFGADLTIMEEGYEFIHRISNDGVLPMITSCSPGWVNYIEHEYPELLDHL
                     SSCKSPHMMLGSMIKSYYAKENNLDPKDIYVVSIMPCVAKKGEKEREENLTDGLKDVD
                     AVLTTRELGKLIKMFGINFRDLKDEDFDQDMFGEYTGAGVIFGASGGVMEAALRTVTD
                     VLTKEDLTNLDYHAVRGEEGVKEASLKIGDMTVNVAVAHSMVLAKPLLEEIKNGTSKY
                     HFIEIMGCPGGCVNGGGQSYVNALTRNSGFDWKQARAKALYDEDLALPVRKSHKNSQI
                     QKLYADFLGEPNSEKAHHLLHTHYTRKERFK"
BASE COUNT          789 a          476 c          358 g          870 t
ORIGIN      
        1 attaaaatat aggcaatttg agggatagtt tgtaagaatg agaaaattgg agctaagtat
       61 tcattaacac ttttaaacaa gccactaaaa acgcctaaga tggtcccaac tattaaagct
      121 aaaaagaaac ttaaagcaac acgtgataaa gttgctccaa ttgcgatata gaaggattga
      181 ctagttgcta ggttgaacat ttggttaaag actacaagtg gtaaaggcaa gattactgct
      241 ttatcaattg ataaagcaaa accttgccat aaaccaataa ttaatattaa agtaattatt
      301 ttacgtctca tgaacttaac atatttttat tataatcaat gttaaataat tttaagaagt
      361 ctttaatttg ctcagaacat tcactagctt ttttataatg gacattttga cgatcgatag
      421 atttaactgt aatttcaaca cttggcaaac tcaatgtctc gataccaatt ttttctagat
      481 agtcttttaa tccattgtaa tcattattag tgaactgatc gattgcttca aatagttcat
      541 catagctgtt attttcttta acaaagatag cggcttgagg ataaccatct ttaccgtatt
      601 ctttttgtaa atcagcaata attgaaagtt caattcctgc ttgtttagct ttagcaatcg
      661 ttgcacttgc aagtggttca gctaacattc caacttgaat gttgcccgat aataattgtt
      721 gttgtactaa tgttgctgat tgatagtact taggagtcaa tgaagtatta atttttacag
      781 tatcaacgat tttttgagga actgcacctt caccgaataa acccagttcg ccagtctgat
      841 ttaatgcttc gttactagtt ccaatatagt ataaattacc ccaggtaata accccttgaa
      901 tacgataatc ggtttgttcg ctagcaatca atttagcacc taaattaatt ggggcaacaa
      961 taatatcgta atcactgcta ttttttgata attcagcaac aagctgatcg ctaccatcaa
     1021 caaaatctat ttttcctttt ttactgatat tttcatactc actgacaaag gcaagtgacg
     1081 gagctcccgc aggacatagt attttaactg tattggattt atctttagtt tctgtatttg
     1141 aacacccaat catcgtgaat atcatcatta aacataataa atatttaatt attttcttca
     1201 tatattcccc cctaacgata attatttaaa ccgttctttt cttgtataat gtgtgtgtaa
     1261 taaatgatgt gctttttcgc tgtttggttc acctaagaag tcagcatata atttttgaat
     1321 ttgtgaattc ttatgggatt ttctaacagg taatgctaag tcttcatcat acaacgcttt
     1381 agcacgagct tgtttccagt caaaaccgct attacgagtt aaagcattaa catatgattg
     1441 accaccaccg ttaacacatc cgccaggaca tcccatgatt tcaatgaaat gatatttaga
     1501 tgtaccgttt ttaatttctt cgagtaaagg tttagctaga accatactat gggcaactgc
     1561 aacatttact gtcatatcac caatttttaa actagcttct ttgactcctt cttcaccacg
     1621 aactgcatga tagtctaaat tagttaagtc ttcttttgtt aaaacatcag ttacagttct
     1681 taaggcagct tccattacac caccactggc accaaagata actccggcac ctgtgtattc
     1741 accgaacatg tcttgatcaa aatcttcgtc tttcaaatca cggaaattaa tcccaaacat
     1801 tttaattaat ttacctaact ctctagttgt taataccgca tcaacatctt ttaaaccatc
     1861 agttaagttt tcttcacgtt ctttttcacc ttttttagca acacaaggca taatagaaac
     1921 aacatagata tcttttggat caaggttatt ttcttttgca taataagatt taatcattga
     1981 acctagcatc atatgtggtg atttacaact tgataaatga tctaataatt caggatattc
     2041 gtgttcaata tagtttaccc atccaggact acaagatgta atcattggta aaactccatc
     2101 attgctgata cgatggataa actcgtatcc ttcttccatt atggtcaaat ctgcaccaaa
     2161 atttgtgtca tatactctat caaagcccat taattttaag gcatggacca ttttaccagt
     2221 tactctagtt cctattggca ttccaaattc ttcgcctaaa ctggctctaa ctgctggtgc
     2281 agtttgcact acaacatgtt tactgtcgtc atttaaagcc gcaataacat tatggacttc
     2341 ttctttttct tgtaacgctc caactggaca aacattgata cattgtccac attgcataca
     2401 gttgacatca ttcatactct tatcataaac tggaccaact ttagttttga aaccacgatt
     2461 cataaagcct aggattccta aaccttgggt ttt
//
LOCUS       DAOMTA010000456         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1916_length_2492_cov_1.909725, whole genome shotgun sequence.
ACCESSION   DAOMTA010000456 DAOMTA010000000
VERSION     DAOMTA010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1916_length_2492_cov_1.909725"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..788
                     /locus_tag="PLK68_11365"
     CDS             240..788
                     /locus_tag="PLK68_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM91043.1"
                     /translation="MPPKTRITKELIIEKSFEITKSEGIENLNARYLAKQLNCSTMPI
                     FKVFNDMNELKFELKKKIDAYYNDFILAHLDKNNYLYTMSFAYIDFAIKDKNLFGALF
                     VNEFIKTRSIEEVVHSAWNRETIEYSAQQFGITIKESEILYRDIRFYTHGIATQIYGG
                     NMALKEEEIQTLLKNAINKFLK"
     gene            785..1246
                     /locus_tag="PLK68_11370"
     CDS             785..1246
                     /locus_tag="PLK68_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91044.1"
                     /translation="MKNLRIIKKYFQVTKANKKITFILVLASLLANGPYMFTSLLFSL
                     TINYLTKQNSKMVIITMILYFVLKIASKLFKIISYNIEKKLYNDVYLKLQNDMIKKLD
                     IINMKYFTNHSKGEILNIVNGDIKLLAEFGTWLSQAILLFQLLFLVKLVSA"
     gene            1246..>2109
                     /locus_tag="PLK68_11375"
     CDS             1246..>2109
                     /locus_tag="PLK68_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91045.1"
                     /translation="MILGCIINSIVIYILNIYNDKFEILTKEGKLKADDEMQFYSQLL
                     NGLRDIKIFSILDQLHAKYQLLNKAYLNIHDKQINNKIISNIISPSITMCTEIILMFY
                     ACYNCLNGKFEIDTVLIIQSYFGILFSSLSDFISTLGELRIKNVSIDRYDNFINSSCN
                     EYFLNDDMIKPNEFCITFKKVTFSYREKTVFQNFNLSLKSNSLIAIIGPSGCGKSTLF
                     NLLLRFEKPTSGKILIGTHPIDKYSRIKYSQILTCVAQHPYLFNMSIYENLALIDPDI
                     NNIKKACQQAEI"
     assembly_gap    2110..2148
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2149..2433
                     /locus_tag="PLK68_11380"
     CDS             <2149..2433
                     /locus_tag="PLK68_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91046.1"
                     /translation="DEYIMSLPKQYDTILSDGALNFSGGQQQRLAIARALLKNSKILL
                     FDEITSALDEKTSYEIFQTLIKLKKTHTILMISHKPSEYQQCDQIINLSF"
BASE COUNT          950 a          335 c          311 g          857 t
ORIGIN      
        1 atatataatt ctattaataa ggctgcttgt attattttaa aacaattaaa aaataattaa
       61 taaaaacact tgacctaaag taactcaaat aaattatagt atgattagat aaaaaagagc
      121 agtatatgta aaatatgttt attaaacatt tatcccttgt atattgacaa agtacatgtt
      181 cttatataaa ataacaattg ttatataaca tatgttattt tatatgaaag gagtcaatta
      241 tgccaccaaa aacaagaata acaaaagagt taattattga aaagtcattt gaaattacta
      301 aatccgaggg gattgaaaat ctaaatgcac gctatctcgc taaacaacta aactgttcaa
      361 caatgcccat ctttaaggtt tttaatgata tgaatgaatt aaaatttgaa ttaaagaaaa
      421 aaattgatgc atattacaac gatttcatct tagcacatct tgataaaaat aattatcttt
      481 acacgatgtc ttttgcttat attgattttg ccatcaaaga caagaatcta tttggagcat
      541 tgtttgtcaa tgaatttatc aaaactcgtt ccattgaaga agtcgtccat tccgcttgga
      601 atcgtgaaac gattgaatat tcagcacaac aatttggtat tacaattaaa gaaagtgaga
      661 tactttatcg tgatattcgt ttttataccc atggaattgc aactcaaata tatggtggta
      721 acatggcttt aaaagaggaa gagatccaaa ctcttttaaa aaatgctata aataaatttc
      781 taaaatgaaa aatctaagaa ttattaaaaa atattttcaa gttactaaag ctaataaaaa
      841 aataaccttt atactggtct tagcttcttt attagcaaat ggtccttata tgtttacaag
      901 tttattgttt agcttaacga ttaattattt aactaaacaa aatagtaaaa tggtcataat
      961 taccatgatc ttgtattttg ttcttaaaat agcatcaaag ttatttaaaa ttatcagcta
     1021 taatattgaa aaaaagctat ataatgatgt ctacctaaaa ttacaaaatg atatgattaa
     1081 aaaattagat attattaata tgaaatactt tactaatcac agcaaaggag aaattcttaa
     1141 tatcgttaat ggtgatatta aattactcgc agagtttggt acttggctat ctcaagctat
     1201 cttattattt caattattat tcttagtcaa attagtatca gcttaatgat cttaggatgt
     1261 atcatcaata gtatcgttat ctatatttta aacatatata atgataaatt tgaaattcta
     1321 accaaagaag gaaaattgaa agctgatgat gaaatgcaat tttacagtca actgcttaac
     1381 ggtttacgag atatcaaaat tttcagtatt cttgatcagt tacatgccaa atatcaattg
     1441 ttgaacaaag cttatctaaa cattcacgat aaacaaatta acaataaaat aatcagtaac
     1501 ataattagtc ctagtatcac aatgtgtaca gaaattattc taatgttcta tgcctgctat
     1561 aattgtctaa atggcaagtt tgaaattgac acagtactca ttatccagtc ttattttgga
     1621 attttattca gctctttaag tgactttatt tctaccttag gtgaactcag aataaaaaat
     1681 gtatcaattg atcgttatga taattttatt aattcatcgt gtaatgaata ttttcttaat
     1741 gatgatatga tcaaaccaaa tgaattttgt attacattta aaaaagttac tttttcttat
     1801 cgtgaaaaaa cagtctttca aaacttcaat ttaagcctaa agtcaaattc attaattgca
     1861 ataatcggtc ccagtggttg tggaaaaagt actttattca atttactttt gcgctttgaa
     1921 aaaccaacta gtggtaagat attgattggt acacatccaa tagacaagta ttctaggata
     1981 aaatattcac agattttaac ttgtgttgcc caacatccgt atttgttcaa tatgtcaatt
     2041 tatgaaaacc ttgcacttat tgatcctgat atcaataata tcaaaaaagc ttgtcaacag
     2101 gcagaaatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cgaatatatt
     2161 atgtcactac caaaacaata cgatactatt ttaagtgacg gtgctttaaa tttttctgga
     2221 ggacaacaac aaaggctagc aattgctcga gcacttctaa aaaattctaa aattttatta
     2281 tttgatgaaa taactagtgc tcttgatgaa aagacttcat atgaaatttt tcaaacatta
     2341 attaaattaa agaagacaca cacaatttta atgatttccc ataaaccaag cgaataccaa
     2401 cagtgtgatc aaattataaa tttatcgttc tagtgttatt gcatctaaaa acattttatt
     2461 tgaactcaca ttaatttttt cacgtattaa aa
//
LOCUS       DAOMTA010000457         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1921_length_2487_cov_2.383635, whole genome shotgun sequence.
ACCESSION   DAOMTA010000457 DAOMTA010000000
VERSION     DAOMTA010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_2487_cov_2.383635"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>315)
                     /locus_tag="PLK68_11385"
     CDS             complement(<1..>315)
                     /locus_tag="PLK68_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM91047.1"
                     /translation="QYGAIEAMRHCDNEIEEMRKAYMLRRNYIVKAFNDLGLHTFTPQ
                     GAFYVFPCIKSTGMTSDQFCEELLKDQLVACVPGTAFGEAGEGFIRVSYAYSIEQIKE
                     ATS"
     assembly_gap    316..325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<326..>864)
                     /locus_tag="PLK68_11390"
     CDS             complement(<326..>864)
                     /locus_tag="PLK68_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM91048.1"
                     /translation="YCYDKECIVTVGGSEAIDLAFRAIINPGDEVILLQPSYVAYTPG
                     VALAGGKVVNIELKEDNEFKLTPELLEAAITPKTKAILLNYPSNPTGGFMTREDYEKI
                     VSIIKKHEIIVITDEIYAELSYEQKFCSIAAFDEIKDQVILVSGYSKAYAMTGWRLGY
                     VLANEVLTKAMNKIHQYVI"
     assembly_gap    865..874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<875..1132)
                     /locus_tag="PLK68_11395"
     CDS             complement(<875..1132)
                     /locus_tag="PLK68_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537082.1"
                     /note="catalyzes the transamination of the aromatic amino
                     acid forming a ketoacid; first step in aromatic amino acid
                     degradation in lactococci; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRM91049.1"
                     /translation="MDFNKVLNDTVREIPPSGIRKFFDLANQMEGVISLGVGEPDFDT
                     PWKIREAAIYSIEQGKTFYTANQGLVELRKEICRYQKRRFGL"
     gene            complement(1132..1623)
                     /locus_tag="PLK68_11400"
     CDS             complement(1132..1623)
                     /locus_tag="PLK68_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM91050.1"
                     /translation="MDENLLLTLLHNNARMDVTDLAMALNETEDNVVDTISQLEKDKV
                     ICGYHTIINWDRTNVDNVMALIQINATPEREYGYDRIAKKIYAFPEVDTMYLLSGSFE
                     FVAIVKGHTMQEAARFVASKLAPLEGVTGTATMFVLKQYKNNGLSFDDDDKEAAERLL
                     VTP"
     gene            complement(2033..>2487)
                     /locus_tag="PLK68_11405"
     CDS             complement(2033..>2487)
                     /locus_tag="PLK68_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HRM91051.1"
                     /translation="QMANVSRKTFYALYHDKYEVIERIVVDNVISDLKGMLELLGKVE
                     LNQPIILEKMYQHFYDEKEFYVKAINIEGNNSLEHCLLYCFEEINLEILKDVEISLVE
                     KEYAAYFFAASQVMLIKKWLINDLELTPREMVLSSHKWAVKAMVNNYF"
BASE COUNT          787 a          497 c          295 g          888 t
ORIGIN      
        1 tgatgtagct tccttgattt gttcgattga gtaagcatac gataccctaa taaatccttc
       61 accagcttcc ccaaaggcag taccagggac acaagcaact aattgatcct ttaataattc
      121 ttcacaaaat tgatcagaag tcattcctgt tgatttaata cacgggaata cataaaatgc
      181 tccttgagga gtaaatgtat gtaatcctaa atcattaaat gctttaacaa tgtaattacg
      241 acgaagcatg tatgcttttc gcatttcttc aatttcatta tcacaatgac gcattgcttc
      301 aattgctcca tattgnnnnn nnnnngatta catattgatg aattttattc attgctttag
      361 tcaaaacttc attagctaat acatatccta aacgccagcc tgtcattgca taagctttag
      421 aatatccgct gactaaaatt acctgatcct tgatttcatc aaatgcagca attgaacaaa
      481 atttttgttc atatgataat tcggcatata tttcatcagt tattacaata atctcatgct
      541 tttttataat tgaaacaatc ttttcataat cctcacgagt cataaatcca cctgttggat
      601 tacttggata gtttaataag attgcttttg ttttaggtgt aatagcagct tctaataatt
      661 caggtgttaa tttaaactca ttgtcttctt taagctcaat atttactacc tttccacctg
      721 ctaaagcaac tcctggagta tatgcgacat aactcggctg taataaaatc acttcatctc
      781 caggattgat aattgcacga aaagcaagat caatcgcctc tgagccccca actgtaacga
      841 tacattcctt atcataacaa taatnnnnnn nnnncaaacc aaatcttctt ttttgataac
      901 gacaaatttc ttttctcagt tctactaaac cttgattagc tgtataaaag gttttacctt
      961 gttcaattga ataaattgca gcctctctaa ttttccatgg agtatcaaaa tcgggttccc
     1021 caacccctaa agatataact ccctccattt gattagctaa atcaaagaat tttcgaatac
     1081 cactaggtgg tatttctctt actgtatcat ttaatacctt attgaaatcc attatggagt
     1141 taccaataat ctttctgcag cttctttgtc atcatcatca aaacttaatc cattattttt
     1201 atattgcttt aatacaaaca ttgttgcagt accagttacc ccttctaatg gtgccaattt
     1261 agaggcaacg aaacgggcgg cttcttgcat tgtgtgtcct ttaacaatcg caacgaattc
     1321 aaagcttccg cttagcaaat acattgtatc tacttctggg aatgcataga tctttttagc
     1381 aatacgatca taaccatact cacgttcagg agttgcatta atttgaatta atgccattac
     1441 attatcaacg tttgtacgat cccaattaat aatcgtatga tatccgcaga taactttatc
     1501 cttttctaat tggctgatgg tatcaacaac attatcttct gtttcgttta atgccatcgc
     1561 aagatccgta acgtccatac gtgcattatt atgaagtaat gttaataata agttctcatc
     1621 catatttctt tcctccaatt tattttactc attatataac attttatgtt tgaggtaaag
     1681 gtgaagaact attacaatca ttattttttt ctttaaaaac cattattcaa agtgacttcc
     1741 aacatttagc ttttcttcct ctttttcacc actatcaccc caattacatt aattgccata
     1801 atctatatag gtaccatctt aatcccatca cttgtagatc ccaataattt tggatatctt
     1861 gtttgccatt attgttaggt atttcttaat caggtacttc aataatttct tctaaaccag
     1921 tcacggccat agctaagttt tcagtgatct taacaataag attctatcgc taaattcagg
     1981 taagactaag cgtaactaaa aaagtaacaa tcattaatat ttgtcacctt ctttaaaaat
     2041 aattattaac catagcttta actgcccatt tatgagaact taacaccatc tctcgtggtg
     2101 taagttctaa atcattaatc aaccatttct ttattaacat aacttgtgaa gcagcaaaaa
     2161 agtaagctgc atactccttt tcgactaaac ttatttctac atcttttaat atctctaagt
     2221 taatttcctc aaaacaatat aaaagacaat gttctaaaga attattcccc tcaatattta
     2281 tagcctttac ataaaactct ttttcgtcat aaaaatgttg atacatcttt tctaatataa
     2341 tcggttgatt taattccacc tttcctaaca attctagcat tccttttaaa tcactaatca
     2401 cattatcaac aacaatgcgt tcaatcactt catatttatc atgatataat gcataaaaag
     2461 tttttctcga aacatttgcc atttgac
//
LOCUS       DAOMTA010000458         2483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1927_length_2483_cov_2.639209, whole genome shotgun sequence.
ACCESSION   DAOMTA010000458 DAOMTA010000000
VERSION     DAOMTA010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_2483_cov_2.639209"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1392)
                     /locus_tag="PLK68_11410"
     CDS             complement(76..1392)
                     /locus_tag="PLK68_11410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM91052.1"
                     /translation="MEYRHAINNRRKLKKKPTIALVVIVVIAILLGGIFWFINREGPY
                     DKYKVYNKDNKKFGTVEHYEKDDDSFFISLYYPKTKNSNLDKIVKDYQENYVKEQKIN
                     KNSKDILYMDYSINEVYNQFINLKFKTIRYDEDDKVVETKEKLFTYDTKKEKILTVGD
                     SLRNTFKTVLASSQGIDKVDAKSNNLTVEKDKLIIYTTEDLKNKIEVNYKDNKELIKL
                     ANKNIPSDAPLDVAGPAAQPEVDPNKKMIAFTLDDGPHKTNTLKVVEMFEKYNGRATF
                     FELGKNITLYPDVVKTVYEHGFEIASHSWDHPDLRKLDAEGLNKQIVDTQNAIYKITG
                     AEPTLIRPPYGAFNDNVKSVVKNNGMEIALWSVDTLDWKLKDANKIKETIINNSYDGA
                     VVLLHDIHNFSVEGLEMALGELYNRGYQFVTLDTLKQYKDLKTVFR"
     assembly_gap    1517..1526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1532..2386)
                     /gene="rsmI"
                     /locus_tag="PLK68_11415"
     CDS             complement(1532..2386)
                     /gene="rsmI"
                     /locus_tag="PLK68_11415"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRM91053.1"
                     /translation="MNRQKSFQNDRPCLYLVATPIGNLEEMTYRAIRTLQEVDYIGAE
                     DTRNTVKILNHYNIRTKLISHHEHNLGQSIPKLINLLLDGNNIALVSDAGYPAISDPG
                     YELVKAAIDNEINVIPISGANACLDALVVSGIAPQPFLFYGFLDHQDKKKKKELQVLK
                     NYQETIVFYESPHRITKTLKLMEDILGDRPIALCREITKKHEEILRGSISEIIKVAGD
                     LKGEMVIVVSGNNNVIEETVFEQTIVEHVDEYVSKGMTVKDAIKEVAKLRNIKKNEVY
                     ATYHQKDE"
BASE COUNT          788 a          435 c          270 g          980 t
ORIGIN      
        1 tgattatatt aaacaattgc ataatagata cattaaaaat atggtttttt gtgtaaaaaa
       61 attcgctagc atatactagc gaaaaactgt tttcaaatct ttatattgtt taagcgtatc
      121 aagagtaaca aattgataac cacggttata aagttctccc aaagccattt ctaatccctc
      181 aacagagaaa ttatgaatat catgtaataa aactactgca ccatcatatg aattattaat
      241 aatagtctct tttattttat tagcatcttt taacttccaa tccaatgtat caacactcca
      301 tagagcaatt tccattccat tatttttgac tactgatttt acattatcat tgaaagcacc
      361 atatggtggt ctaattaatg taggttcagc accagtaatc ttataaattg cattttgtgt
      421 atcaacaatt tgtttattaa gtccttcagc atccaacttt cttaaatctg gatgatccca
      481 agagtgacta gcaatctcaa aaccatgttc atagactgtt tttaccacat caggatataa
      541 agtaatattt ttccctaatt caaagaaagt tgcacgacca ttatattttt caaacatttc
      601 aactactttt aaagtatttg ttttgtgtgg tccatcatca agagtgaaag caatcatttt
      661 cttgtttgga tcaacttcag gctgagcagc aggtcctgca acatctaaag gtgcatctga
      721 aggtatattc ttattcgcta atttgattaa ttctttatta tctttataat taacttcaat
      781 cttgtttttc aaatcttcag tagtgtaaat aattaattta tctttttcta ctgttaaatt
      841 gttactttta gcatctactt tatcaattcc ttgactacta gctaacactg ttttaaaagt
      901 atttcttaaa ctgtctccaa cagttagaat cttttctttt tttgtatcat aagtaaataa
      961 tttttctttt gtttctacta ctttgtcatc ttcatcataa cgaatagttt taaattttaa
     1021 gttaataaac tgattatata cttcattgat tgagtagtcc atgtataata tatctttact
     1081 atttttatta atcttttgtt cttttacata attttcttga taatctttaa caattttatc
     1141 taaattactg ttttttgtct ttggataata taatgaaata aagaaactat catcgtcttt
     1201 ttcatagtgt tctactgttc caaatttctt gttatcttta ttatatactt tatacttatc
     1261 atacggtccc tcgcggttaa taaaccaaaa tattcctcct aataaaattg ctataacaac
     1321 aattactact agggcaattg ttggtttttt ctttaatttt cttctattat ttattgcgtg
     1381 cctatactcc ataactttcc catcctttcg ccatacatat aatacgatat ttgtcgaatt
     1441 aaatcaaatt tattgtattg ctaataaaac atctttttta atgtttcacg tgaaactttt
     1501 aagaatttta tttaatnnnn nnnnnnacga attactcgtc cttttgatga tatgtagcat
     1561 atacttcatt tttcttaata tttcttaatt tagcaacctc tttaattgca tccttaacag
     1621 tcattccttt gctaacatat tcatcaacat gttcaacaat agtttgctca aaaactgtct
     1681 cttctataac attattattg ccagaaacaa caataaccat ttctcccttt aaatcaccag
     1741 caactttaat aatttcactt atagatccgc gtaaaatttc ctcatgtttt ttagttattt
     1801 cccgacaaag tgcaattggt cgatctccaa gaatatcttc cattaattta agtgtcttag
     1861 taatacgatg cggagattcg taaaaaacaa tcgtttcttg ataattcttt aatacctgca
     1921 actctttttt cttcttctta tcttgatgat ctaagaagcc ataaaataaa aaaggttgcg
     1981 gagcaattcc agaaactact aatgcatcta aacaagcatt agccccacta attggaatta
     2041 catttatctc attatcgatt gcagctttta ctaattcata acctggatca gaaatagctg
     2101 gataaccagc atcactaact aatgcaatat tattaccatc aagcaacaaa ttaatcaatt
     2161 taggaatcga ttgccctaaa ttatgctcat gatgggaaat taatttagtt ctaatattat
     2221 aatgatttaa tattttaaca gtatttctgg tatcctcagc accaatataa tcaacttcct
     2281 gtaaagttct aattgctcga taagtcattt cttctaaatt accaattggt gtggcaacta
     2341 aatataaaca tggtcggtca ttttgaaaac ttttttgtct attcatcaat attttctcca
     2401 aacatttttc ttacctcatt actataacca ccatctgcat tatgtgcatt taaaggtggt
     2461 tcaattttta atcctttttt tcc
//
LOCUS       DAOMTA010000459         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1932_length_2480_cov_2.757938, whole genome shotgun sequence.
ACCESSION   DAOMTA010000459 DAOMTA010000000
VERSION     DAOMTA010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_2480_cov_2.757938"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="argC"
                     /locus_tag="PLK68_11420"
     CDS             <1..999
                     /gene="argC"
                     /locus_tag="PLK68_11420"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRM91054.1"
                     /translation="GCELVRFLLTHKEVEIKWVSSRTYTDEEYSSVYSSYFKLLDQQC
                     VDEDITKYLDDVDVVFFATPQGVCAKMLNEEVLSKVKVIDLSADYRIKDVKTYEQWYG
                     IDHASPQFIDEAVYGLCEINRDKVKQARLIANPGCYPTCSILSVYPLAKADLIDMDTL
                     IIDAKSGVSGAGRGAKVANLYCEVNESIKSYGVASHRHTPEIEEQLGYAANQEVLLNF
                     TPHLIPMNRGILITAYAKLKKGVNEVDVAKAYQCYDDEYFVRVLKSGVLPEVRSVKAS
                     NFVDINYKVDPRTNRVIMMGAMDNLVKGAAGQAIQNMNIMFGLDEKEGLMLAPAIL"
     gene            1013..2236
                     /gene="argJ"
                     /locus_tag="PLK68_11425"
     CDS             1013..2236
                     /gene="argJ"
                     /locus_tag="PLK68_11425"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRM91055.1"
                     /translation="MKIIENGTVTSPKGYLGAGEHVGIKKAKKDLALIYSKVDAKAVG
                     TFTQNIVKAAPVLWDKMIVDNYDHVRIIAVNSGVANACTGDEGLKCNEEFAQTVADNF
                     GLEKEEVLICSTGVIGKQLPMNKIINGVPIVKPLLSESQEAGVIVAESIMTTDTKPKH
                     LAVEIEVGGKTVTLGACCKGSGMIHPNMATMLGFITTDCNISKELLNKALKEVIPDTF
                     NMVSVDRDTSTNDTVLLLANGLAGNQEIVIEDQDYQIFKEALYYVNEYLAKAIAGDGE
                     GATKLLEVQVHNADTVQQAKVIAKSVCTSPLVKTAVYGNDANWGRLLCAMGYSGEQFD
                     PYNVDLSVASEFGELQLVAKGMATDYSEELATKILSSSEVKAIIDIHNGDCKAIAWGC
                     DLTYDYVKINADYRS"
     gene            2264..>2480
                     /locus_tag="PLK68_11430"
     CDS             2264..>2480
                     /locus_tag="PLK68_11430"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536092.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRM91056.1"
                     /translation="MHTVEQNKAQVLVDALSYIQKFQGDIVVIKYGGSAMTNEVIKHS
                     VLKDIAVLKSVGIKPVIVHGGGNDINGW"
BASE COUNT          842 a          306 c          572 g          760 t
ORIGIN      
        1 ggatgtgagt tagtccgatt tttattaact cacaaagaag tggaaattaa gtgggtttca
       61 tcaagaacct acactgatga ggagtatagt tcggtttatt ccagctattt taaattattg
      121 gatcagcagt gtgttgatga ggatattaca aaatatcttg atgatgttga tgtggttttc
      181 tttgcaactc cacaaggggt ttgtgccaaa atgcttaatg aggaagtttt aagcaaagta
      241 aaagtgattg atttaagtgc agactatcga attaaagatg ttaagacata tgaacaatgg
      301 tatggaattg atcatgcttc accacaattt atcgatgaag ctgtatatgg gctgtgtgag
      361 ataaatcgtg ataaagtgaa acaggcacga ttaatagcta atccaggatg ttatccaact
      421 tgttcaattt tatcagtcta tccgttagct aaggcggatt taattgatat ggatactttg
      481 atcattgatg ctaaaagtgg tgttagtggg gctggccgtg gggctaaagt cgcaaactta
      541 tattgcgagg taaatgaaag tatcaagtct tatggggttg caagtcatcg tcatactccg
      601 gaaattgaag aacagttagg ttatgcggca aatcaagaag tattattaaa cttcacgcct
      661 catttaattc caatgaatcg aggaatctta attacagctt atgcaaaact taaaaaggga
      721 gttaatgaag tggatgtcgc aaaggcttat caatgttatg atgatgagta ttttgtaagg
      781 gtattgaaat ctggggtctt accagaagta cgtagtgtta aggctagtaa ttttgttgat
      841 attaattata aagttgatcc acggacaaat cgagttatta tgatgggagc tatggataac
      901 ttagttaaag gagcagctgg ccaagcaatt caaaatatga atattatgtt tggattagat
      961 gaaaaagagg ggctaatgct tgctccagca atcttataag ggggaaaata aaatgaaaat
     1021 aattgagaat ggaacggtaa cctcgcctaa aggatattta ggtgctggag aacatgttgg
     1081 tattaaaaaa gcaaaaaaag atttagcatt gatctatagt aaagttgatg ccaaagcggt
     1141 aggaactttt acccaaaata ttgttaaggc agcaccagtg ttatgggata agatgatcgt
     1201 tgataattat gatcatgtta gaatcattgc tgttaatagt ggggtcgcaa atgcttgtac
     1261 tggcgatgaa ggtttaaaat gtaacgaaga gtttgctcaa actgtagcag ataattttgg
     1321 attggaaaaa gaggaagttc taatttgttc tacaggtgta attggtaagc aattaccaat
     1381 gaataaaatc attaatgggg taccaattgt taaaccgtta ttaagtgaga gccaagaagc
     1441 aggggtaatc gtagctgaat caattatgac gacagatacg aaaccaaagc atcttgctgt
     1501 tgaaattgaa gtcggtggaa agacggttac tttgggggct tgctgtaagg ggtcaggaat
     1561 gattcatcca aatatggcaa ccatgctggg atttattaca accgattgta atatttctaa
     1621 ggaattatta aataaggcat tgaaagaagt aattcctgat actttcaata tggtttctgt
     1681 tgatcgtgat acttcaacta atgatacagt cttgttatta gctaatggat tagctggaaa
     1741 tcaagaaatt gttatagaag atcaggatta tcaaatattt aaagaagcct tatattatgt
     1801 taatgaatat ctagctaaag caattgccgg tgatggcgaa ggtgcaacta aattgttaga
     1861 agttcaagtt cataatgctg atacagttca acaggctaaa gtaatcgcta aaagcgtttg
     1921 tacttcaccg ttagtaaaga cagcagtata tggcaatgac gctaactggg gaagattatt
     1981 atgtgcaatg ggttattctg gtgaacaatt tgatccatat aatgttgatt tgagcgttgc
     2041 tagtgaattt ggggaattgc aattggtggc taaaggaatg gcgactgatt atagtgaaga
     2101 attagcaact aagatcttat caagcagcga agtaaaagca attattgata ttcataatgg
     2161 tgattgtaaa gcgattgcat ggggatgtga tctaacttac gattacgtta aaatcaacgc
     2221 tgactatcgt tcataataaa aatttaatta agggggaaat aatgtgcata cagttgagca
     2281 aaataaagca caagtattag tagatgcttt aagttatatt caaaaattcc aaggagatat
     2341 cgtagttatt aaatatggtg gtagtgccat gactaatgaa gttattaaac acagtgtttt
     2401 aaaagatatt gcggttctaa aatcagttgg gattaaaccg gtaattgttc atggtggggg
     2461 gaatgatatc aatggatggt
//
LOCUS       DAOMTA010000460         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1935_length_2480_cov_2.032577, whole genome shotgun sequence.
ACCESSION   DAOMTA010000460 DAOMTA010000000
VERSION     DAOMTA010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1935_length_2480_cov_2.032577"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>221
                     /locus_tag="PLK68_11435"
     CDS             <1..>221
                     /locus_tag="PLK68_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91057.1"
                     /translation="NNEDQMACLNRLCKEYDPQTIAVNVSNNFAFSDGLTQGLYEMIT
                     AKMAPEYAERIVRNDMLAIKLMELRTPLE"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <232..933
                     /locus_tag="PLK68_11440"
     CDS             <232..933
                     /locus_tag="PLK68_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRM91058.1"
                     /translation="PEVMEVAFSVIEEAFSSKSIIPGVTTCEDLQWLMMQRVKDLGLD
                     YWFEPTVDLQRPGLDNPRYFGVIEKGDLLHCDFGIKYLNICTDTQRLAYIAKDDEESI
                     PQELLDGMKVNDRFQDIVAENMADGKSGNDVLIDSLKQGKDEGIEAVLYSHPCNIYGH
                     GPGTTIGLWNNQKAIPVKGDVLMSYDTTYALELNTKSKAFGQDYYFYTEETVAFTRDG
                     LIYLHPGRKNIYFIK"
     gene            942..2156
                     /locus_tag="PLK68_11445"
     CDS             942..2156
                     /locus_tag="PLK68_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRM91059.1"
                     /translation="MKNRMKKEFLGFEGGLFSEVEKADVGDSFAKLAENGVALMGWAD
                     PFMPDFSIPEHIMEKTLEAIKSPVAAHYTAPTGNMELRAMICQKAKKMYGVDLNPARN
                     VIITPGSDSALFFAMYPFLEKGDEVIIPCPSYPNNMQNITMMQATPVVLELNEAEDYQ
                     INQQALESLVSNKTKMIVLTHPNNPTTTVYNHESLMAIREVVLKHDLVLVCDQAFEDF
                     TFENEFIAPMALDGMFEHTVTVCSISKGMGLSGYRVGYIMASDVIMDVMYGCAVSVIG
                     ATNTVSQIAAIEAFKHPEFMDEFNRAYDIRRHQAYNILNTVPGVSMELPASGFLAWVD
                     VSALGDSSAICKRLISEAKVAVNDGINYGPGGTGHLRIVLGVYRDDQQVIEALTRMAA
                     TLDAIAKEKGIK"
     gene            complement(2181..>2480)
                     /locus_tag="PLK68_11450"
     CDS             complement(2181..>2480)
                     /locus_tag="PLK68_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91060.1"
                     /translation="RIAMYFVINCFISAVCIISLILFTEVFPKWTKLLVGAVQVVTTS
                     YAIAILIKKIGEWTSVWSGDYKYINSNFLWILPIVAVITIAVAYICNKKSKKAAK"
BASE COUNT          821 a          326 c          516 g          807 t
ORIGIN      
        1 aataatgaag atcaaatggc ttgtttaaat cgtttatgca aagaatatga tccacaaaca
       61 attgcagtca atgtttcaaa taattttgct tttagtgatg gattgacaca ggggttatat
      121 gaaatgatta cagcaaaaat ggctccagaa tatgctgaac gaatcgtacg taatgatatg
      181 ttggcaatta agctgatgga gttgcgtact ccattagaat tnnnnnnnnn nccagaagtt
      241 atggaggttg ctttttctgt gattgaagaa gcttttagtt caaaaagtat tattccaggg
      301 gttactactt gtgaggactt acaatggttg atgatgcagc gtgttaaaga tctaggatta
      361 gattattggt ttgaaccaac tgttgattta caacgacctg gtttagataa tccccgatat
      421 tttggtgtaa ttgaaaaagg tgatttatta cattgtgatt ttggaattaa atatttgaat
      481 atttgtacgg atactcaaag attggcttat attgctaaag atgatgaaga gagtattcca
      541 caggaattgc ttgatggaat gaaggttaat gatcgttttc aagacattgt ggctgaaaac
      601 atggctgatg gtaaaagtgg taatgatgtt ttgatagatt ctttgaaaca aggtaaagat
      661 gaaggaattg aagctgtatt atattctcat ccatgtaata tttatggtca tggaccaggg
      721 acaacgattg gtttatggaa taatcaaaaa gcaattcctg ttaaaggtga tgtgttaatg
      781 tcatatgata caacatatgc acttgaatta aatacaaaga gcaaagcttt tggccaagat
      841 tattatttct atactgaaga aacagtagca tttactcgag atggattaat ttatttacat
      901 ccgggacgta aaaatattta ttttattaag taggtactaa gatgaaaaat agaatgaaaa
      961 aagagttttt agggtttgaa ggcgggctat tcagcgaggt agaaaaagcg gatgttggtg
     1021 atagttttgc taaattagct gaaaatggag tagcattaat gggatgggcc gatcctttta
     1081 tgcctgactt ttcaattcct gagcatatta tggaaaaaac acttgaagca ataaaatctc
     1141 ctgtagcagc gcattacact gctccaacgg gaaatatgga gcttagggca atgatttgtc
     1201 aaaaagccaa gaaaatgtat ggggtagatt taaatccggc tagaaacgta attattacgc
     1261 ctggaagtga tagtgcttta ttctttgcaa tgtatccttt cttggaaaaa ggtgatgaag
     1321 taatcattcc ttgtccaagt tatcctaata atatgcaaaa tattacaatg atgcaggcta
     1381 cgccggtagt tcttgaatta aatgaagctg aagattatca aattaatcaa caagcattag
     1441 aatcactagt tagtaacaag actaagatga ttgttttaac tcatcctaat aatccgacta
     1501 caacagtcta taatcatgaa agtcttatgg caattaggga agtggtttta aaacatgatt
     1561 tagtacttgt ttgtgatcag gcttttgaag attttacatt tgaaaatgag tttattgcac
     1621 cgatggcttt agatggtatg tttgaacata cagttaccgt ttgttcaatt tctaaaggga
     1681 tgggattaag cggttatcga gttggctata ttatggcaag tgatgtaatt atggatgtga
     1741 tgtatggttg tgcggtaagt gtgattgggg caactaatac tgttagtcaa attgctgcaa
     1801 ttgaagcttt taagcatcct gaatttatgg atgaattcaa ccgtgcttac gatattcgtc
     1861 gtcatcaagc ttacaatatt ttaaatacag tacctggggt ttcaatggaa ttgccagcct
     1921 ctggtttctt agcctgggtt gatgttagcg ctttaggcga tagtagtgcg atttgtaagc
     1981 gtcttattag tgaagctaaa gtggcggtta atgatggaat taattatgga cccggtggaa
     2041 caggacatct acgtattgta ttaggtgttt atcgtgatga tcaacaagtt atcgaagcat
     2101 taactcggat ggcagcaaca ttagatgcaa ttgctaaaga aaaaggtata aaataaaaac
     2161 atgaatttga attcatgttt ttattttgct gctttttttg attttttatt acagatgtac
     2221 gcaactgcaa tagtaattac agcaacgata ggtaggatcc ataagaaatt actattaatg
     2281 tatttataat ctcctgacca taccgaagtc cattcaccga tttttttaat tagaatagca
     2341 atagcataag aagttgtaac aacttgtacg gcaccaacta ataatttagt ccatttagga
     2401 aagacttcag tgaataaaat taaagaaata atacatactg cactaataaa acaatttatt
     2461 acgaaataca tagcaattct
//
LOCUS       DAOMTA010000461         2479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1936_length_2479_cov_2.243812, whole genome shotgun sequence.
ACCESSION   DAOMTA010000461 DAOMTA010000000
VERSION     DAOMTA010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_2479_cov_2.243812"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..2217
                     /locus_tag="PLK68_11455"
     CDS             250..2217
                     /locus_tag="PLK68_11455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HRM91061.1"
                     /translation="MKNIFLLLFFLLFIGKNLFAEEDNVEKKSNIHQQAMNTNWCDNT
                     TGEWLIGFFDNYVIFDNQLWNYENKTIKGDRYIFHVAHGRKEIDIKIGKLKSNKRNII
                     IGCIDHNCSPITTSILPDYQTKDNRNSFKDNGYRLRDSVTIQGVVRNAPNKLSAITIY
                     QYSPFEPCLPSADLLHNVPIDSLGRFYIRIPVYNSNLFDVYKIPQQLPLEVDEDYFFY
                     FDIANNQHLVMGRNARLVNELIAHPINTLEIINGRKVDINQRLNAINQANNNCISCLD
                     SIISRHVNLSERYKIWWRERIRNSYAFSLLSDEDMLNRKVEENEIISIINEGDYLNPQ
                     LPLTLYADAYDIINGYMTRLKNITVKANLGDVINDGYFKIGDDVKPMIRKLKEVHELA
                     QKGDTIAARHIYEVDIKAIPNYEKLQDSLNEILDPMLEKVQNVKYLNTIMLKADSMKL
                     RSDLRDLYFTNELVLNMNGTPAWLQLSQWARANVKSPWMLNFFNEQNAFFLDISNEAS
                     NMKFDDNKSLEELTKGSDIFNHIIKPFKGKIIYVDVWGTWCGPCRQEMIYVPALKESL
                     KDKDIVYIYLCNGSTDESWRNAIKRYHLEGDNCVHYNLPGKQENALERYLRVNGYPSY
                     RLVNRQGKLLQGEAPQPSCTEVLKSTINSIK"
BASE COUNT          857 a          412 c          448 g          762 t
ORIGIN      
        1 ttgaactttc ttcatctgat tgaacagatg ttttttctaa ttttcgttga tatttttagt
       61 attacagaat tgctaatata tagatttaaa gatgctatca tttctactta atatataaag
      121 taacctgttt tttagaggta ttatcatatc ttttgaaaat aatgatacct atataaaagt
      181 aattgataca tttaaaaaaa taaaatacca cgaaaagaag taactttact gaataaatca
      241 aaataccaaa tgaaaaatat ttttctatta ttattttttc ttttgtttat aggaaaaaac
      301 ctgtttgccg aagaggataa tgttgaaaaa aaatcaaata ttcatcaaca agcgatgaac
      361 actaattggt gtgataatac tacaggggaa tggttgatag gtttctttga taattatgtt
      421 atttttgata atcaactatg gaactatgaa aataaaacaa taaaagggga cagatatata
      481 tttcatgtag cacatggccg aaaggaaatt gatataaaaa ttggcaaatt aaagagtaac
      541 aagcgtaata tcattatcgg ttgcatagat cacaattgct cgcctatcac gactagcatc
      601 ctgccagact accagacgaa agacaaccga aacagtttca aggataatgg ttatcgcctt
      661 agggacagtg tcacaataca aggcgttgtt cgcaatgcac ccaataaact ttctgctata
      721 acaatctacc aatatagtcc ttttgaacca tgtcttccta gtgctgattt gcttcacaat
      781 gtgccaattg attctcttgg tcggttctat attcgaattc ccgtgtataa ttcaaattta
      841 tttgatgttt acaaaattcc tcagcaatta cctttggagg ttgacgaaga ttatttcttt
      901 tatttcgata ttgccaacaa tcaacatcta gtaatgggca gaaatgcccg tttggttaat
      961 gaactaatcg cacatcctat aaatactttg gaaataataa atggaagaaa agttgacatc
     1021 aaccaaaggc ttaatgcaat caatcaggct aataataatt gtatttcatg tctcgattct
     1081 atcatttcac gtcacgtaaa cctctcagaa cgatacaaaa tatggtggcg tgagcgaatt
     1141 cgcaatagtt acgcattctc tttgttgtct gatgaggata tgctcaatag gaaagtagaa
     1201 gaaaatgaga taatcagcat tataaatgaa ggtgattatc taaatccaca attgccactc
     1261 acactctacg ctgatgcata cgatattata aacggataca tgacaagatt gaaaaatata
     1321 actgtcaaag ccaacttagg tgatgtcatt aatgatggtt attttaagat aggagacgac
     1381 gtaaagccaa tgatcagaaa attgaaagag gttcacgaat tagctcaaaa aggagacaca
     1441 attgcagcaa ggcatattta tgaagtagac ataaaagcca tacctaatta tgaaaaattg
     1501 caggatagtc tgaacgaaat actcgatccc atgttggaaa aggttcagaa tgtcaaatat
     1561 ctaaatacta ttatgttgaa agcggattca atgaagcttc gttctgattt aagagactta
     1621 tattttacaa atgaattagt cctcaatatg aacggcactc ctgcatggtt acagctatct
     1681 caatgggcac gggctaacgt taagtcgcca tggatgctga atttctttaa cgagcagaat
     1741 gcattctttc tagatatttc taatgaggca tcgaatatga aatttgatga taataaatct
     1801 ttggaagaac ttacaaaagg ttcagatatt ttcaaccata taattaagcc cttcaaagga
     1861 aagattatct atgttgatgt gtgggggaca tggtgcggtc cttgcaggca ggagatgatt
     1921 tacgttcctg cattaaagga gtcgctcaag gataaagaca tcgtttatat ctatttatgc
     1981 aacggtagca ctgatgagtc gtggcgcaat gccatcaaac gataccatct tgagggcgac
     2041 aactgcgtgc actacaatct gcccgggaaa caagaaaatg ccttagaaag atatctgcgc
     2101 gtcaatggat atccatctta ccgcctcgtc aatcgccaag gcaaactttt acaaggggaa
     2161 gctccacaac caagttgtac tgaagtactt aaaagtacaa taaattcgat aaaataaaca
     2221 gcagtcttaa aataacaata aaactatctt taagatatta cttgttctga caatctttat
     2281 ctagtaggct tttaagaaag tctatatgaa ggacgacgta tttcgtaata ttttttgctt
     2341 gctacgtgcc gtttcttatt ggcggtagca tgctttttgt atacttgtgt tggaggatcg
     2401 catacgctgc tggtgcatat ccttcggatt tggcttctga aagactcatt gaaatgcaac
     2461 tgcgacttaa ataacggca
//
LOCUS       DAOMTA010000462         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1939_length_2476_cov_2.373399, whole genome shotgun sequence.
ACCESSION   DAOMTA010000462 DAOMTA010000000
VERSION     DAOMTA010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1939_length_2476_cov_2.373399"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLK68_11460"
     CDS             complement(<1..564)
                     /locus_tag="PLK68_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRM91062.1"
                     /translation="MNKREINQKLIVNYKNHLIDEEKSLATITKYIRDIEKFYQYADK
                     KEVTKELVKLYKEELMKSYKPTSINSMLAALNQFFEYNGWLECKIKELKIQKRVFLEE
                     SKELSKDEYKRLVNAARKQKNERLYVLLQAICSTGIRVSEHRYITVQALKDGYAQIYN
                     KGKVREIFFSDDLKRILLKYCHKNKIEN"
     gene            complement(710..1522)
                     /locus_tag="PLK68_11465"
     CDS             complement(710..1522)
                     /locus_tag="PLK68_11465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM91063.1"
                     /translation="MNVKLIAVDMDGTFLTNNNEYDQERFTRQYEFMKEKGIHFVVAS
                     GNQYYQLRSFFPDIHNELAFIAENGAYIVDQGKDVFVAKLAKEDIQTVLKVVNQYPEV
                     EKIVCGRKSAYISKYIDEDFYNTMNFYYHRLAKVDNFDDFDDTIFKIYLHCSKDNFET
                     ILTELKEKIGHIMTPVDCGHFGIDLIIPGINKAHGINLLMERWKISDAETMAFGDSGN
                     DLEMLEQATYGFAMANAKEAIKKIADYTISSNEEHGVLEVVDWYISKKKMFE"
     gene            complement(1650..>2476)
                     /locus_tag="PLK68_11470"
     CDS             complement(1650..>2476)
                     /locus_tag="PLK68_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRM91064.1"
                     /translation="YAYRFLSSIENVEAVKIKAKFNGATGNYSAILTAFPNEDWQVLA
                     KKFVEEYLGLTFNPLTTQIESHDYTCHILDGIRHFNNVLVDFDVDMWLYISMEYFKQI
                     PVKGEVGSSTMPHKVNPIRFENSEANIDMSNNICIALSNKLPKSRMQRDLSDSSSQRN
                     LGLAFGYSLQAINETMNGLAKCVVNKDKLASDLNEKWEVLAEPIQTMLRKYGVPDAYD
                     TLKALTRGKSISKEDILKFAESLDILSDQDRQTLVDMTPASYIGLAKELAKIELNK"
BASE COUNT          813 a          438 c          270 g          955 t
ORIGIN      
        1 attttcaatt ttatttttat gacaatattt taataagatt ctttttagat catcactaaa
       61 aaaaatttct cttacttttc ctttattata tatttgcgca tacccatcct taagtgcttg
      121 gactgtaatg tatcgatgtt cgctaacacg tattccagtt gagcaaattg cttgcagtaa
      181 aacatataga cgctcgtttt tttgctttcg agcagcatta actaatcgtt tatattcatc
      241 ttttgatagt tctttgcttt cttctaaaaa aactcttttt tgaattttta gttctttgat
      301 tttacattct agccaaccat tatattcaaa aaactgattt aatgctgcaa gcatactatt
      361 tatgctagta ggcttatatg atttcattaa ttcttcttta tataatttaa ctaattcctt
      421 agtaacttcc tttttatccg catattgata aaatttttca atatcccgta tatatttagt
      481 tattgttgct aaactttttt cttcatcaat tagatgattt ttgtaattaa cgattaattt
      541 ttgatttatt tctcttttat tcatctataa taatctcctt aatctcaata atctaatata
      601 aaataataac atatacatct catattatta aataaatcat atatattatt ggatttttta
      661 cgaaaaataa acaaacaaaa agaagaagta atttatcttt acatcttctt tattcaaaca
      721 tctttttttt acttatatac cagtcaacta cctctaatac accatgttcc tcattagagg
      781 aaattgtgta atccgcaatt ttcttaattg cttcttttgc atttgccatg gcaaaaccat
      841 atgtagcctg ctcaagcatc tctaaatcat taccactatc cccaaatgcc attgtttcgg
      901 catcactaat tttccaacgt tccattaaca ggttaattcc atgtgcttta ttaatcccag
      961 ggataatcaa atcaattcca aaatgtccac aatcaactgg tgtcataata tgtccgatct
     1021 tctctttcaa ttctgttaaa atcgtttcaa aattatcttt tgaacaatgc agatatatct
     1081 taaaaatagt atcatcaaaa tcatcaaagt tatctacttt agcaagacga tgatagtaaa
     1141 agttcattgt attataaaaa tcttcatcta tgtatttact aatataagca ctttttcgac
     1201 cacagacaat tttttctact tcaggatatt gattaactac ctttaaaact gtctgaatat
     1261 cctcttttgc aagttttgca acaaaaacat cttttccttg atcaacgata taagctccgt
     1321 tttcagcaat gaaagccaac tcgttatgaa tatctggaaa aaaagatctt agttgataat
     1381 attgattccc actagcaact acaaaatgaa ttcccttttc cttcataaat tcgtattgcc
     1441 tcgtaaatcg ttcttgatca tattcattat tatttgttaa aaaagttcca tccatatcta
     1501 ctgctattaa ttttacattc atatttacat cctttccatc tttcgttata atgtgacgta
     1561 tctttatata acttagtata tattgtttat aaaaagattg tagattattt cataaaatga
     1621 aaaaatgact ggaaaccagt cattttctac tatttattta attcgatttt agcaagttct
     1681 ttagctaaac caatataaga agccggtgtc atatcaacta aagtttgacg atcttgatct
     1741 gataagatat ccagactttc agcaaatttt agaatatcct ctttagaaat acttttacct
     1801 cttgttaaag cttttaaagt atcataagca tcaggtactc catactttct taacattgtt
     1861 tgaattggtt ctgctaatac ttcccatttt tcatttaagt cactagctag tttgtcttta
     1921 ttaactacac acttagccaa cccattcatc gtttcattga tggcttgtaa agaataccca
     1981 aatgctaaac ctaaatttct ttgacttgaa gagtctgata agtctctttg cattcttgac
     2041 ttaggaagtt tattagataa agcaatacag atattattag acatatcaat attagcttca
     2101 ctgttttcaa agcgaatagg atttacttta tgtggcatag tactgcttcc aacttcacct
     2161 ttaactggaa tttgtttaaa gtattccatt gaaatataca gccacatatc aacatcaaaa
     2221 tcgactaata cattattgaa atgacgaata ccatccaaaa tatgacaagt ataatcatga
     2281 ctttcaattt gagtagtcaa tgggttaaat gttaaaccta agtactcttc aacaaatttt
     2341 tttgctaaaa cttgccaatc ttcattaggg aaagccgtta aaatagcact atagttacct
     2401 gttgcaccat tgaacttagc tttgatttta actgcttcaa cattttcaat actagacaaa
     2461 aaacgataag cataaa
//
LOCUS       DAOMTA010000463         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1940_length_2476_cov_2.353986, whole genome shotgun sequence.
ACCESSION   DAOMTA010000463 DAOMTA010000000
VERSION     DAOMTA010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_2476_cov_2.353986"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..723)
                     /locus_tag="PLK68_11475"
     CDS             complement(172..723)
                     /locus_tag="PLK68_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRM91065.1"
                     /translation="MRKAAVLVVDGYEESETVTIVDLLRRAGIECHTFGFAEQYVRGM
                     QGMMIKVDKIFSDEIKNYDMLVLPGGRPGGVNLGANPLVIEMVQYYNENGKYLAAICS
                     GTIVLSKARVIDGKNVTGYTGYADKLVGGEFVDKVVVFDQNIITSQGPATSYPFAFKI
                     IEVLGQDVSEMKERLLYNFAGGR"
     gene            complement(725..1285)
                     /locus_tag="PLK68_11480"
     CDS             complement(725..1285)
                     /locus_tag="PLK68_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRM91066.1"
                     /translation="MKKAAVIVAPGFEEGETLSIIDIIRRANFQCDMIGFEKDVAGAH
                     DIIVKCDSVLNDEVIDYDMIILPGGYPGAANLRDNTRLIEILNIMAQKNKYICAICAA
                     PIVLEKAGLLKGKNYTAYVGYEQKIKQGNYLHDKVVIDGKIVTSRGPATVYAFAYKLV
                     DLLGGNSLALKKRMVYFNAFDVKEDE"
     gene            complement(1420..2142)
                     /locus_tag="PLK68_11485"
     CDS             complement(1420..2142)
                     /locus_tag="PLK68_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM91067.1"
                     /translation="MKDSQAFNLLNQFLAINTYNEKKKYDLILVLGNAIAETGLIAYQ
                     LYKTGHAYHFMIAGGKGHTTDILINTIKEKYDIKDITKRSEAELFKYLIEENYGVDNT
                     ILLEKESTNCGENIQFAFKLLKKEDIIVKNILLVHDPLMQRRIDATARHYAPHINFDN
                     YRCFLPVVENIGFELKNNIWGLWSKERYISLLLGEMKRVIDDKDGYGPNGKQYIEHVE
                     VPQKILAAYRYIFFRYGKYQRK"
     gene            complement(2132..>2476)
                     /locus_tag="PLK68_11490"
     CDS             complement(2132..>2476)
                     /locus_tag="PLK68_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91068.1"
                     /translation="CKVRAIQLSTLQRCAAHLASYIDIENALRIGSYAINCALDKRTG
                     IMVTISKNRNEFLLSHVDIHKVANHVKPFPLEWIDEKNKKIKKEYIDYVVPLIYKSGQ
                     IELPQYLQLNER"
BASE COUNT          775 a          522 c          273 g          906 t
ORIGIN      
        1 acattgcatt ccttagcaat ttccttgatt ccaagatttt gaacttcctc aaggtgattc
       61 aaaatatatg ctgctatctg gctattagtt gaaccatttt tttcgctgac aagcgtactt
      121 aaaaaaacta tcggtaattt atcatagatc attattatca ctcactttca cttatcttcc
      181 accagcaaag ttatataata gtctttcctt catttcacta acatcttgac ctaacacttc
      241 gataatctta aatgcaaatg gatatgatgt tgctggccct tggcttgtta taatattctg
      301 atcaaaaaca acaaccttat caacaaattc tccacctact aatttatcgg catatccagt
      361 atagccagtc acatttttac cgtcgataac tcttgccttt gataaaacaa ttgttccaga
      421 acatattgct gctaaatact ttccattttc attatagtat tgaaccattt caataaccag
      481 tggatttgct cctaaattca caccccctgg tcttccgcca ggtaaaacca gcatatcata
      541 atttttgatc tcatcactaa aaatcttatc tacttttatc atcatcccct gcatccccct
      601 aacgtactgt tcagcaaaac caaatgtatg acactcaatt ccggctcttc ttaacaagtc
      661 tacaatcgtc actgtttcac tttcttcgta tccatctact accaatactg cagcttttct
      721 catactattc atcctcctta acatcaaaag cattaaaata gaccattctt tttttgagag
      781 ctagtgaatt gccacccaat aaatctacca gcttatatgc aaatgcataa actgttgctg
      841 gaccgcgact cgttacaatt ttcccatcaa tcaccacttt atcatgtaag taatttcctt
      901 gttttatttt ttgctcgtat cccacataag cagtatagtt ttttcctttt agtaatcccg
      961 ccttttctaa aacaatcgga gctgcacata tggcacaaat atacttgttt ttttgtgcca
     1021 ttatatttaa aatttcaatt aaccttgtat tatctcttaa gttagcagca ccgggatacc
     1081 ctcctggtaa aataatcata tcataatcaa ttacctcatc atttagtacg ctgtcacatt
     1141 tcacaataat atcatgtgct cctgcaacat ccttttcaaa accgatcata tcgcattgaa
     1201 aattagcacg acgaataata tctataatag ataacgtctc cccttcttca aaccctggag
     1261 ctacaataac tgctgccttt ttcatatctt ctttcctcct tttaatttat atttacacaa
     1321 taatattgtt tacaccgttt atcaattcat ttccaaaatt aagaaatcat aaaaaagact
     1381 tttgtttcac caaaagtcta aatattattg ataaagtttt tatttccttt gatatttacc
     1441 atatcgaaaa aagatatatc tatatgccgc cagtatcttt tgaggaactt caacatgttc
     1501 aatatactgc ttaccatttg gtccatagcc atctttatca tcgatgactc tcttcatttc
     1561 tcctaataaa agtgaaatat atcgttcttt tgaccataag ccccaaatat tatttttaag
     1621 ttcaaatcca atattctcaa caactggcaa aaaacatcta taattatcaa aatttatatg
     1681 tggagcataa tgtcttgcag ttgcatcgat tcgcctttgc ataagaggat catgaaccaa
     1741 taaaatattt tttactataa tgtcttcttt tttgagaagt ttgaaagcaa actgaatatt
     1801 ttccccacaa ttagtagatt ctttttctaa taaaatagta ttgtctaccc cataattttc
     1861 ttcaataaga tacttaaata attctgcttc acttctttta gtaatatctt taatatcata
     1921 cttctcttta atcgtattga ttaaaatatc tgtagtatga ccttttcctc cagcaatcat
     1981 gaagtgataa gcatgccctg ttttgtatag ttgataagct attaatccag tttcagcaat
     2041 agcatttcct aatactaaaa tcagatcata ctttttcttc tcattatatg tatttatcgc
     2101 taaaaactga ttaagaagat taaaagcctg actatctttc atttaattgc aaatattgag
     2161 gaagctcaat ttgtccactt ttatatatta aaggaaccac ataatcaata tattcttttt
     2221 taatcttctt attcttttca tcaatccatt ctaatggaaa tggtttaaca tgatttgcta
     2281 ctttatgaat atcaacgtga cttaataaaa attcatttct attttttgaa attgtgacca
     2341 taatccccgt tcgtttatcc aacgcacaat ttattgcata actaccaata cgtaaagcat
     2401 tttctatgtc tatgtaactg gctaaatgtg ctgcacaacg ctgaagggta cttaactgaa
     2461 tggcccgtac tttaca
//
LOCUS       DAOMTA010000464         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1942_length_2472_cov_2.376500, whole genome shotgun sequence.
ACCESSION   DAOMTA010000464 DAOMTA010000000
VERSION     DAOMTA010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_2472_cov_2.376500"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PLK68_11495"
     CDS             <1..1413
                     /locus_tag="PLK68_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRM91069.1"
                     /translation="NYNGNYILYDMVKDENLYSVTLPLPATEYFYGYFVTYSDGSAVV
                     VQDPVNPSKKNEINNHDATWSYFYVGNSSDALAGQSYIYPRNDNMGYYQYDTYIAYDG
                     TENCLVIYLPNGYSLGNNYKTIYLAHGNGGNEIEWCQLGSAGNIVDNLIAEGELADSI
                     IVTLNNSHFSGTGFDIKSNVILAQDVVNNVIPFIEKNYKVSTDPKDRAYAGLSAGGVA
                     ASTVMEIAPDSFGYFGIISAAVQIDDEVFTDELISKLQTKKIYFSAGTVDFGLINSFF
                     KASILDFMLPKLDAENIDYTFEIQNGGHDWNTWRGAFTTFAKDILWNQENIEYCITDG
                     ANKNIKQGEELEIKTDIPSSLFKMLIFDDQEIDRSKYTVSGDLITIILPKELISTLTI
                     GEHILVIIANEGQAATMFNITADTNVLDIVENDQITKQSSHTAVLAPKTDDPSLFGVY
                     CLFILLSGGAIVVIKKKYFN"
     gene            1492..1632
                     /locus_tag="PLK68_11500"
     CDS             1492..1632
                     /locus_tag="PLK68_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91070.1"
                     /translation="MDEEILESFLTFIQRCNSEAVNSNDYNIIQNVFKYIGKKHSLLL
                     MN"
     gene            1752..2285
                     /locus_tag="PLK68_11505"
     CDS             1752..2285
                     /locus_tag="PLK68_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91071.1"
                     /translation="MKVFLSQNILFKSNEEISEYLFQQCLDNLVATKENLDLATLLKI
                     VQLFKESEDITFLGDEHDLDEFLLLQIKLLTSGKCAYLFKVSETKTVRSYFFNENSVV
                     VIINVCDGFFHYHDALENAKEVGAKVIYISQDYNKEVADKVDIFYRYGVEWSINTGYV
                     SLYYIGELLEKLCIRNF"
BASE COUNT          867 a          290 c          452 g          863 t
ORIGIN      
        1 aattataatg gaaactatat tctctatgat atggtaaaag atgaaaattt atattctgtc
       61 accttaccat taccagcaac agaatatttt tatggatatt ttgtaactta tagtgatgga
      121 tctgcagtag ttgtgcagga tcctgttaat ccttcaaaaa agaatgaaat taataatcat
      181 gatgctactt ggtcctattt ttatgttgga aatagttcag atgcattagc gggtcaatcc
      241 tatatttatc caagaaatga taatatgggt tattatcaat acgatacata catcgcttat
      301 gatggaactg aaaactgtct tgtgatttat ttaccgaatg gttatagttt agggaataac
      361 tataaaacga tttatttagc acatggtaat gggggcaacg aaattgaatg gtgtcaatta
      421 ggatcagcag gtaatattgt tgataatcta attgctgaag gagaactagc tgatagtata
      481 atcgtgacat taaataattc gcattttagc gggacaggat ttgatattaa atcgaatgtc
      541 atattggctc aagatgttgt taataatgta attcctttta ttgaaaagaa ttataaggtt
      601 tcaactgatc ctaaggatcg agcttatgcg ggattatcag ctggtggtgt tgcggcaagt
      661 acagtaatgg aaattgcacc tgatagtttt ggatattttg gtattatttc agcagctgtt
      721 caaattgatg atgaagtatt tacagacgaa cttatttcaa aattacagac aaagaaaatc
      781 tatttttcgg caggaacagt tgattttggg ttgattaatt ctttttttaa ggcctctatc
      841 ttagatttta tgttacctaa attagatgct gaaaatattg attatacttt tgaaatacaa
      901 aatgggggac atgactggaa tacgtggcgt ggagctttta caacatttgc taaagatatc
      961 ctatggaatc aagaaaatat tgaatattgt ataacagatg gagctaataa aaatattaaa
     1021 cagggagaag aacttgaaat aaagactgac ataccatcgt cattattcaa aatgctcata
     1081 tttgatgatc aagaaattga tagatcaaag tatactgttt ctggtgattt aataaccatt
     1141 attttaccaa aagaacttat ttcaacatta acaattggag aacatatatt agtaataatc
     1201 gccaatgagg gacaagcagc tacaatgttt aatattactg ctgatactaa tgttctagat
     1261 atagtagaga atgatcagat aactaaacaa tcatcacata ctgctgtgct ggcacctaag
     1321 acggatgatc cttcgctgtt tggagtttat tgtctattta ttttgttaag tgggggagca
     1381 atagttgtaa taaagaaaaa gtattttaat tgatatgagt ctaatatttt gaagactcat
     1441 attcttttat attatcaaga tgttataata tagggaaagg agctgataaa aatggatgag
     1501 gaaatactag aaagtttctt aacctttatt caaagatgta acagcgaagc tgttaatagt
     1561 aatgattata atattattca aaatgtattt aagtatattg gaaaaaaaca ttcccttcta
     1621 ttaatgaatt agctcgagag gcgaatgtat ctaaggcaag tatttcaaga tttataaaaa
     1681 aatatagttt tgaaaattat cagcaattta gaaccctatt atcaactcaa acaacactct
     1741 tagattataa tttgaaagta tttttatcac agaatattct ttttaaaagt aatgaagaaa
     1801 tcagtgaata tctttttcaa cagtgtcttg ataatcttgt tgcaacaaaa gagaatcttg
     1861 atttagcaac gcttttaaaa attgttcagt tatttaagga aagcgaggat attacttttc
     1921 taggtgatga gcatgatctt gatgaattcc ttttgttgca aatcaaatta ttgacttcgg
     1981 ggaagtgcgc atatctgttt aaagtcagtg aaacgaagac ggttaggagc tattttttta
     2041 atgaaaattc agttgttgtt atcattaatg tatgtgacgg tttttttcac taccatgatg
     2101 cgttagaaaa tgcaaaagaa gttggggcta aggttattta tataagccag gattataata
     2161 aagaggttgc tgacaaggta gatatttttt atcgttacgg agtagagtgg tcaataaata
     2221 ctggctatgt atcactgtat tatattggtg agttactgga aaaattatgt attcgtaatt
     2281 tttagacaaa taatttataa tgatgtaagc agcctcaaaa aatataaccc taatactaag
     2341 ttataatgat agggtgtgct tatctaacct tatttagtat tatttttaag atttgtgatt
     2401 attagatata taatcaatta taattacatt gaatagattt attttggtta tctaggtatg
     2461 gctatagaag ac
//
LOCUS       DAOMTA010000465         2469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1946_length_2469_cov_2.222038, whole genome shotgun sequence.
ACCESSION   DAOMTA010000465 DAOMTA010000000
VERSION     DAOMTA010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_2469_cov_2.222038"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLK68_11510"
     CDS             complement(<1..618)
                     /locus_tag="PLK68_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM91072.1"
                     /translation="MQEKPNTNNKRPGKSPQKNHNNKSKSRNNTPKKAVNKSRQTKNS
                     IDTKVFALGGLNEVGKNMYCVEHDNELFIIDAGVKFAEEGLPGIDYVIPDYSYLKRNE
                     KKIRGLMITHGHEDHIGGIPFLLQVVKIPFIYATPLASAMIKRKLDEKRLTNATKIIQ
                     IDDEYQIKSKYFNIGFFKTNHSIPESMGIIINSPNGRIVETGDFKF"
     gene            complement(697..1563)
                     /locus_tag="PLK68_11515"
     CDS             complement(697..1563)
                     /locus_tag="PLK68_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM91073.1"
                     /translation="MNKVAIITDSNSGITQAEGTKLGINVLPMPFTIDGQTFYEDINL
                     SQDEFYKKLMTDAEVFTSQPVVGDVSKLWDEVLKEYDEIVHIPMSSGLSGSCQTALML
                     AGEYDGRVQVVDSQRVSVTQKWDVLDALELSKAGKSAKEIKEILEANKLNASIYITVN
                     TLDYLRKGGRITPAVAILGGMMKIKPILQIQGEKLDTFSKTRTMSKATKIMMEAIKKD
                     IDERLDPEGKGKNVHVCIAYTYDEQPALELKKELEAIHPDSTIICDPLSLSVSCHIGP
                     HALAIAACKKII"
     gene            1747..2112
                     /locus_tag="PLK68_11520"
     CDS             1747..2112
                     /locus_tag="PLK68_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM91074.1"
                     /translation="MQIDNNSSVPIYEQIYKQLLEYITTGLMQADDKLPSVRELSSSI
                     HINPNTVVKAYRLLEEQGFIYSLPGKGSFVSSKQNNQTKLFEQQIKVLTKEIVTKSKY
                     IGLTFEQLCQFMEQTWEGK"
     gene            2112..>2469
                     /locus_tag="PLK68_11525"
     CDS             2112..>2469
                     /locus_tag="PLK68_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91075.1"
                     /translation="MLLVKELSKKIDHKQILDNITLNIPKGSIYGIIGENGAGKTTLI
                     RHLVGAYQGDTGFVELEGMRIYENPEAKAKLVYIPDEFFDTFGHNIRDIKELYQGIYP
                     SFNEARYLRLMKMFKHD"
BASE COUNT          815 a          390 c          382 g          882 t
ORIGIN      
        1 aaatttaaaa tctcctgttt caacaatccg tccattagga ctattaataa taattcccat
       61 tgattctgga atagaatggt ttgttttaaa gaatccaata ttaaaatatt tagatttgat
      121 ttgatactca tcgtcgattt gaataatttt tgtagcatta gttagtcttt tttcatctaa
      181 ctttctttta atcatggcac tagcaagtgg tgtagcataa ataaaaggaa ttttaactac
      241 ttgaagtaag aaaggaattc cacctatatg atcttcatga ccatgggtaa tcattaaacc
      301 acgaattttc ttttcatttc tttttaaata cgagtaatca ggaataacgt aatcaattcc
      361 cggtaatcct tcttcagcaa attttactcc ggcatcaata atgaataatt cattatcgtg
      421 ttcaacacag tacatatttt taccgacttc atttagtccg ccaagggcaa ataccttagt
      481 atcaatacta ttttttgttt gtcttgattt atttaccgct ttttttggcg tgttgtttcg
      541 agatttactt ttattattat gatttttttg tggtgacttg ccaggtcgtt tattatttgt
      601 attaggcttt tcttgcataa taacctcctt aattatattt tgtatccttt taagtattat
      661 aacacatatt aaaagacaag aaacgaagtt tcttgcctag ataatctttt tgcatgcagc
      721 aatggctaga gcatgcggtc caatatgaca tgaaacacta agtgacaatg ggtcacaaat
      781 aattgttgaa tcaggatgaa tagcttctaa ttctttcttt aattctaatg ctggctgttc
      841 gtcgtatgta tatgcaatac aaacatgaac attctttcct tttccttctg gatcaagacg
      901 ttcatcaata tcttttttta ttgcttccat cataatttta gttgctttag acattgttct
      961 ggtcttagag aaagtatcga gcttttcacc ttgaatttgt aagattggtt tgattttcat
     1021 cattccgcct aaaattgcaa cagcaggagt gattcttcca cctttacgaa gataatctaa
     1081 cgtattaaca gtaatataga tagaagcatt taatttattt gcttcaagaa tttctttgat
     1141 ttcttttgct gatttaccag ctttagataa ttcaagtgca tccagaacat cccatttttg
     1201 agttactgaa acgcgttgtg aatcaacgac ttgaactcgt ccatcatatt cacctgctaa
     1261 catcaaagca gtttggcaag agccgcttaa gccactagac atgggaatat gaacaatttc
     1321 atcatactct tttaatactt catcccaaag tttactaaca tcaccaacta cgggttgact
     1381 tgtaaaaact tcagcatctg tcattagttt tttataaaac tcatcttgag ataagttgat
     1441 atcttcatag aaggtctgac catcaatagt aaatggcatc ggtagtacgt tgattcctaa
     1501 ttttgtacct tcagcctgag tgattccaga attactatca gtgataattg ctactttatt
     1561 catttaaaaa cctcctatct ctaaaccata ttttacttga aataataaaa aatgcaaact
     1621 atttaaccat tattataaaa ataattaaat gaaaggcatt acaatatata actgtattat
     1681 atattgtaat acagttatat atttggtata ctacatttgt aagaatgtag taggaaagga
     1741 gttattatgc aaatagataa taatagcagt gttccaattt atgaacaaat ctataaacag
     1801 ctgctcgaat atataacgac agggttaatg caggcggacg ataaactacc ctctgtccgt
     1861 gaattatctt catcaattca tattaatcct aataccgttg ttaaagccta tcgtctttta
     1921 gaagagcaag gttttattta ttccttgccg gggaagggaa gttttgttag ctcaaaacaa
     1981 aataatcaaa cgaagttgtt tgaacaacaa attaaagttc tcacaaaaga gattgttact
     2041 aaaagtaaat atattggttt gacctttgaa caattatgcc aatttatgga acagacttgg
     2101 gagggaaaat aatgttatta gtcaaagaat tatcaaagaa aattgatcac aaacaaattt
     2161 tggacaatat tactttaaat atacctaaag gttcaattta tggaattatt ggtgaaaatg
     2221 gtgcaggtaa aacaactttg attcgtcatt tagtaggtgc ttatcaaggt gatacaggat
     2281 ttgttgaatt agaaggaatg cgtatttatg aaaatcctga agcaaaagca aaattagtat
     2341 atatccccga tgaatttttt gataccttcg gacataatat tcgggatatt aaagaattat
     2401 atcaagggat ttatccaagt tttaacgagg cacggtattt aagattgatg aaaatgttta
     2461 agcacgata
//
LOCUS       DAOMTA010000466         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1957_length_2464_cov_1.923620, whole genome shotgun sequence.
ACCESSION   DAOMTA010000466 DAOMTA010000000
VERSION     DAOMTA010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_2464_cov_1.923620"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>959
                     /locus_tag="PLK68_11530"
     CDS             98..>959
                     /locus_tag="PLK68_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRM91076.1"
                     /translation="MSYKFKITRGNAILNFSEEYCQSRCQLIESESFARVLKGFVHEG
                     KRKENTTYTYLREVKSESVEIVSDLRTIFKLLLVMSVEEVLSSYPHLSGYFRDRDEFV
                     KVIEEVYLFWRKLQRYSVVYNENTSRGYQNVQFGDAQAKFEELVLSVYRTIEETALGY
                     RHHVYRQITAGVNAGLRVTNMRSFLPYEYRNLDDIPFIETVIIHPPFITYSKRNKRDG
                     VFPETKHNPIEDLKFDSEQWFCYPAKVGDLLAFVYFNVAFMAQGVALCNLFELASEEE
                     YRNRKPDIIYV"
     assembly_gap    960..969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <970..1938
                     /locus_tag="PLK68_11535"
     CDS             <970..1938
                     /locus_tag="PLK68_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRM91077.1"
                     /translation="QGEVISESHRNNDKYEKRSKRTIDVYGYEDGKMNQSFYQDDEND
                     MMVALLSASDDFDYFGYMKKMMLTLHNVRKINQRQLPIHGAMVKITLHSGEVKNIVVM
                     GDSGAGKSETIEQIKVFGAAYIRDLVTVYDDMGVLLLDEEGKVKTSGTEIGAFVRLDD
                     LDAGYSFKELDRSVFMNPDKVNARIVIPITDYKDVVAHHDVDFFLYANNYEEGGESLS
                     FFDDVNSALDVFRAGNRMAKGTTTEYGLVGSYFANPFGPVQREEQTEVILVDYFNRMF
                     KQGVKVGQLRTRLGIKGNEHKGPQEAATAILKYITGEDELKQLEEE"
     gene            2071..>2464
                     /locus_tag="PLK68_11540"
     CDS             2071..>2464
                     /locus_tag="PLK68_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRM91078.1"
                     /translation="MKKYKLLLIILVLVITLIILSIKPTNQIVLYNYHPVIEISNKFD
                     PQANIQKVIGGSISDVNVDTSKIDFSKVGKYPITYIYNNIKTTITIELQDTLKPNVEV
                     QEVTVDLGMKITARDVVKDIYDNSRTTVK"
BASE COUNT          874 a          298 c          482 g          800 t
ORIGIN      
        1 ttataataag tattgtatac aatgtgcctt ttgtgttaaa atgacaaaaa tcaataagtg
       61 ggggaagtag cttgttgaag aaagaaaggg tggtaaaatg agttataaat ttaaaattac
      121 tcgcgggaac gcgatcttaa acttttctga agaatattgc caatctcgtt gtcaattaat
      181 cgaaagtgaa tcttttgcac gagttttaaa aggattcgtt cacgaaggaa aaagaaaaga
      241 aaatactact tatacttatt taagagaagt aaaaagtgag agtgtggaaa ttgttagtga
      301 tttaagaaca atttttaaat tgttattagt gatgtctgtt gaagaagttc tatcttcata
      361 tccacattta tcaggatatt ttagagatcg tgatgaattt gtaaaagtaa ttgaggaagt
      421 atatcttttc tggagaaaat tacaacgtta tagtgttgta tataatgaaa atacaagtag
      481 aggatatcaa aatgttcagt tcggagatgc tcaagctaag tttgaagaat tagttttatc
      541 agtataccgt actattgaag aaacagcttt gggatatcgt catcatgtat accgtcaaat
      601 tactgcgggg gttaatgcag gtttaagagt aacaaatatg cgttcattct taccttatga
      661 atatcgtaat ttagatgata ttccatttat tgaaaccgta attattcatc caccattcat
      721 tacttattct aagcggaata aacgtgatgg ggtattccca gaaacaaaac ataatccaat
      781 cgaagatttg aaatttgatt ctgaacaatg gttctgttat ccagctaaag ttggagattt
      841 attagcattt gtatatttca atgtagcatt tatggctcaa ggggttgctt tatgtaactt
      901 atttgaatta gcaagtgaag aagaatatcg taatcgtaaa ccagatatca tctatgtatn
      961 nnnnnnnnnc agggagaagt gattagtgaa tcacatagaa ataatgataa atatgaaaag
     1021 cgcagcaaaa ggacgataga tgtatacggt tatgaagatg gtaagatgaa tcaaagtttc
     1081 tatcaagatg atgaaaatga tatgatggta gcattattat cagcaagtga tgattttgat
     1141 tactttggtt atatgaaaaa aatgatgtta actttacaca atgttcgtaa gatcaatcaa
     1201 cgtcaattac ctatccatgg agcgatggtt aaaattacgt tacattctgg tgaagttaaa
     1261 aatatagtag ttatgggtga cagtggcgct ggtaaatcag aaacaattga acaaattaaa
     1321 gtatttggag cagcttatat ccgtgattta gtaactgtat atgatgatat gggtgttcta
     1381 ttattagacg aagaaggtaa agttaaaact tctggtacag aaattggagc atttgtacga
     1441 cttgatgatt tagatgcagg gtatagtttt aaagaattag accgttctgt ttttatgaat
     1501 ccagataaag taaatgcgcg aatcgtaata ccaatcactg attataaaga tgttgttgcg
     1561 catcatgatg ttgacttctt cctatatgca aataactatg aagagggtgg cgaatcatta
     1621 agtttctttg atgatgtaaa tagtgcttta gatgtattta gagcaggaaa tcgtatggct
     1681 aaaggaacta ctactgaata cggtttagta ggcagctatt ttgctaatcc atttggacca
     1741 gtacaacgtg aagaacaaac tgaagttatt ttagttgatt atttcaatag aatgtttaaa
     1801 caaggggtta aagtaggaca attaagaaca cgtttaggaa ttaaaggaaa cgaacataaa
     1861 ggtcctcaag aagcagctac ggcaattctt aaatatatta ctggtgaaga tgaattaaaa
     1921 caattagaag aagaataaat cttagataaa tggtacatcc cgtgtctgct tgacgaggga
     1981 tgtaccacga tactaagatt tttttgttta tcctcacgga gcagttcttt ttttttataa
     2041 gggtgaatat ggtttgatga ggggatcaaa gtgaaaaagt ataaactact tctaattatt
     2101 ttagttctag ttattacctt gataatatta tcaattaaac caactaatca aatcgtttta
     2161 tacaactatc atcctgtcat tgaaatatct aataaattcg atcctcaggc taatattcaa
     2221 aaagttattg gcggctcaat tagtgacgtt aatgtagata ctagtaagat tgattttagt
     2281 aaagtcggta aatatccgat tacgtatatt tataataaca tcaaaactac gattacgatt
     2341 gaacttcagg atactttaaa gccaaatgtt gaagtacagg aagttactgt tgatcttggt
     2401 atgaagataa ctgctcggga tgtagtaaaa gatatttatg ataattctcg gacaacagtt
     2461 aaat
//
LOCUS       DAOMTA010000467         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1960_length_2463_cov_2.416944, whole genome shotgun sequence.
ACCESSION   DAOMTA010000467 DAOMTA010000000
VERSION     DAOMTA010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_2463_cov_2.416944"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    308..317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<318..1582)
                     /locus_tag="PLK68_11545"
     CDS             complement(<318..1582)
                     /locus_tag="PLK68_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91079.1"
                     /translation="MGKYRQQIKSICFLAWPAVVQEAMNVVVTYVDTAMVGALGAGAS
                     AAVGLTSTVGWLVSSIAVAFGIGILSVCAQAVGANNDEKVKRVGQQALFLTLIVGIAL
                     TIMCVLIAPYLPTWLNGDKAIRGEAASYFMIISIPLLFRTAALILASALRGVSDMKTP
                     MLINLYMNIINIILNFLLIYPTRELFGIIIPGAGLGVNGAAIATAISFVAGGIMMFLR
                     YYKNTLFDLKNSGFHFYKKEFKECLNIGIPVVLERSVICLGHVTFASLIAKLGVVRFA
                     AHTIAIQAEQAFYIPGYGFQTAAATLVGNAVGQKDEHKVKEVTYLISGITMFLMIICG
                     IALFIFAEQLMGIFTPDHEVITLGARVLRIVSISEPLYGILVILEGTFNGMGDTKAPF
                     VFSLFTMWGIRVTGSWLMINVFHQSIEAV"
     gene            complement(1756..>2463)
                     /locus_tag="PLK68_11550"
     CDS             complement(1756..>2463)
                     /locus_tag="PLK68_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HRM91080.1"
                     /translation="SSIVNDGHGFIECAHGTISVPVPATSEIFAASNVVARQIDIDTE
                     LVTPTGAAIIAELSSSFQPMPPMNVQKIGWGCGTKELNIPNVLKVSLGTIDKTNDEII
                     VMETNIDDCSSEILAYTAEKLFENGALDVFFTPIYMKKNRPAYRLSTACKEDKLELLQ
                     NIIFRETTTIGIRYRREERKILARKAIELDTPYGKIAAKEVTNNDETYVYPEYESIKR
                     LAKEHDLAVKEIYKLIK"
BASE COUNT          846 a          507 c          303 g          797 t
ORIGIN      
        1 caaaaaatca aataattgga aaatcgtttt ttatggtaaa gtatagtttt aaatttaaga
       61 atatcgctta aaaagtagtt ctaaattata attatcatat tatttaatat ctaaaatttt
      121 tacttttttc aaattcatat tttcttatat taaatattta ctatgaaatc taaattcacg
      181 tatatttaaa agcaggtata tcgaataaga tacacccgct tttaatcaac tattaagacg
      241 gtacttccag ccatttttta aaaatcttcg cgataataag agacatctgg ctatattatc
      301 aaataccnnn nnnnnnncaa actgcttcaa tactttgatg gaaaacattg atcattagcc
      361 aagaaccggt taccctaatt ccccacatcg taaataatga aaaaacaaat ggtgccttag
      421 tatcacccat cccattgaat gttccctcaa gaattactaa gatcccataa agcggttctg
      481 aaatagatac tattctcaat actcgagccc ccaaagtgat aacctcatgg tcaggagtaa
      541 aaatccccat taactgttca gcaaatataa ataatgcaat cccacaaatt atcattaaaa
      601 acattgtaat accactaatc aaataagtta cctctttaac tttatgctca tctttttgtc
      661 caactgcatt tccaactaaa gttgctgccg ccgtttgaaa accatacccg ggaatataaa
      721 aagcttgttc tgcttgaatt gcaattgtat gagcagcaaa tcgtactact cctaacttcg
      781 caatcaagga agcaaaagtt acgtgcccta aacaaataac actacgttcc aatactactg
      841 gaatcccaat attcaaacat tccttaaact cttttttata aaaatgaaaa ccactgtttt
      901 ttaaatcaaa tagtgtattt ttatagtaac gtaagaacat cataattcct ccagcaacaa
      961 atgatatagc cgtcgcaatc gctgctccat ttacccccag tccagcaccg ggaattataa
     1021 ttccaaataa ttctcgtgtc ggataaatta acaagaaatt taaaattata ttaataatat
     1081 tcatatataa atttattagc attggtgttt tcatatcact gacaccacgt aaagccgatg
     1141 ccaaaatcaa agctgctgtc ctaaataata atggaattga aataatcata aagtatgatg
     1201 ccgcttcacc tctaattgct ttatcaccat ttagccaagt tggtaaatat ggtgcaatta
     1261 aaacacacat tatagttaaa gctatcccaa caattaatgt caaaaataga gcttgctgcc
     1321 ctacccgctt tactttttca tcattatttg ctcctactgc ttgagcacaa actgataaaa
     1381 tcccaattcc aaaagcaaca gcaatactag acactaacca tccaaccgtt gaggtcaatc
     1441 caactgcagc cgatgcccca gctcctaatg cccctaccat cgctgtatca acatatgtaa
     1501 cgacaacatt catggcctct tgaactaccg ccggccaggc taaaaaacaa atcgatttaa
     1561 tttgttgacg atattttccc actatctcac tcctacctaa tctaataaaa aaagaccatt
     1621 aaatggcttt aattgtatat actattatac acttttttaa tatagataga tagccccaaa
     1681 caaaagctta gtgttaaaat taaatttatt taataaatcc tccaattcat aattaaatcg
     1741 gaggatcaaa atctattatt ttattagttt atatatttct ttgactgcaa gatcatgctc
     1801 cttagctaat cgttttatac tttcgtattc cggatataca taagtctcat cattattagt
     1861 tacttcttta gcagcaattt ttccatatgg tgtatccagc tcaattgctt ttcgagctaa
     1921 aatttttctt tcttcacgac gataacgaat tccaattgtt gttgtttctc taaatataat
     1981 attctgtaat aattctaatt tatcttcttt acatgctgta ctcaatcgat aggctgggcg
     2041 attctttttc atataaatcg gtgtaaagaa gacatctaaa gctccatttt caaaaagctt
     2101 ttcagcagta taagcaagta tttcactcga acaatcatct atatttgttt ccattacaat
     2161 aatttcatcg ttagttttat cgattgttcc taaagatact ttcaaaacat taggaatatt
     2221 taattcttta gttccacaac cccaaccaat cttttgaaca ttcataggag gcatcggttg
     2281 aaacgatgaa ctcagctcag caataatagc cgcccctgtc ggtgtcacta attctgtatc
     2341 aatatcaatc tgacgggcta ctacattact agcagcaaaa atttccgatg tcgctggtac
     2401 cggaactgaa attgttccat gagcacattc aataaaacca tgcccatcat taacgatact
     2461 gct
//
LOCUS       DAOMTA010000468         2462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1961_length_2462_cov_2.338596, whole genome shotgun sequence.
ACCESSION   DAOMTA010000468 DAOMTA010000000
VERSION     DAOMTA010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_2462_cov_2.338596"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PLK68_11555"
     CDS             complement(<1..1356)
                     /locus_tag="PLK68_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM91081.1"
                     /translation="METKHKRSSFSGRLGYVMAVAGSAVGLGNIWRFPYLAAKYGGGT
                     FLFTYFILAITFGFALLISETALGRKTKKSPIAAYKQLGAKKLQFGGWLNAIVPMLIV
                     PYYCVVGGWVCKYLFEFITNNSASLVSDTYFTGFSSSTLQPVIWLLVFAGMVFGVVML
                     GVEKGIEKCSKVLMPALVAMALFIALYALFTPGAIEGVKYYLIPDFSRFSIMTVVAAM
                     GQMFYSLSIGMGILFTYGSYMKREIDMEQSITQVEIMDTLIAFLAGLMIIPAVFAFSG
                     GNPDTLNAGPSLMFITMPKVFASMSFGNIIGLVFFLLVLFAALTSAISLMECCVSIIQ
                     DRYDFSRKKCCVLIFGGIALLGIPCSLGFGVLDFITPLGLSILDFFDFMTNSIMMPIS
                     AACTCLLIIKVTGFKTVTDEVEYSSKFKRKKAYLFCMKYVVVPGLLIILISSVLSTMG
                     II"
     gene            complement(1618..>2462)
                     /locus_tag="PLK68_11560"
     CDS             complement(1618..>2462)
                     /locus_tag="PLK68_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM91082.1"
                     /translation="KLFLDVVGYVDIKNNYSLYRDNQQYHHYLNHFKLGGYKLFLDGS
                     PQGKTAWLSKPYENSGDYCGYPIYLDSDVKKFVDMAINDKAQLLTHCNGDAASNQLLD
                     AFNDQYTPLRPVMIHSQTLRPDQLPRLKSIGMIPSFFIAHIYYWGDIHRRNLGQRAES
                     ISCVHSALKLDLPYTFHQDTPVIEPNMLETIWCAVKRQTKNGVTLGTDEQISVYNALK
                     GVTINAAYQYFEEHLKGSIKEGKKADLLILDRNPCKVAIEEIRQIKIIETIKDGITIY
                     KKDI"
BASE COUNT          876 a          429 c          393 g          764 t
ORIGIN      
        1 aataatcccc attgttgata agactgaact aattaatatt attaataggc cagggacaac
       61 aacatatttc atacaaaata gataagcttt tttacgcttg aatttagaac tgtattcaac
      121 ttcgtcagta actgttttaa aaccggttac tttgatgata agtagacatg tacaagctgc
      181 agaaattggc atcatgattg agtttgtcat aaaatcaaag aaatcaagaa tacttaaacc
      241 aagcggtgta atgaaatcta aaacaccaaa tccaagtgaa catggaatac ctaataaagc
      301 aattcctcca aaaataagaa cacagcattt ttttcttgaa aaatcatatc gatcttgaat
      361 aattgaaaca caacattcca ttaatgaaat tgctgaagtt aatgctgcaa acagtactaa
      421 taaaaagaat actaatccaa taatgttacc aaatgacata ctggcaaata cttttggcat
      481 tgtaataaac attaaggaag gaccggcgtt tagtgtatct ggatttccac cagaaaaagc
      541 aaatactgcg gggataatca ttaaacctgc tagaaaggca attaatgtat ccattatctc
      601 tacttgagta attgattgtt ccatatcaat ttcacgtttc atatatgaac catatgtaaa
      661 taaaattccc atcccgatag acaacgaata gaacatttgt cccatagccg ctactactgt
      721 catgattgaa aaacgcgaga agtcaggaat taaatagtat ttaacccctt caatagcacc
      781 aggtgtaaat aatgcatata aagcaatgaa tagagccatt gcaactaaag caggcattaa
      841 tactttactg catttttcaa ttcctttttc aacacctagc ataactacgc caaataccat
      901 tccagcaaag acaagtaacc agatgacagg ctgtagtgtt gaagatgaaa aaccagtaaa
      961 ataggtatca ctaactaagc tggcagaatt gtttgtaata aattcaaata aatatttaca
     1021 aacccatccc ccaacaacac agtagtaagg tacgatgagc attggcacga tggcatttag
     1081 ccaaccacca aattgaagtt tttttgcacc caattgttta taggctgcaa tcgggctctt
     1141 ttttgttttt cttcccaagg cagtttcact aattagcaaa gcaaaaccaa aggtaatcgc
     1201 taaaataaaa tatgtgaata aaaaagtacc tccaccatat ttagctgcta aataagggaa
     1261 acgccaaata ttcccgagcc cgacagcaga accagctact gccataacat agcccaggcg
     1321 accagaaaag ctacttcgct tgtgttttgt ttccattttt atctcctatt attaaatttt
     1381 catgcctttt cattataata aaatttgtct ataaaagtca attaaataaa tggcactaaa
     1441 taggatgttt ttgttatgat tttgttaatg ctgagttaaa ttagatcaat agtgttatga
     1501 tataaatata aataagtaaa gaagggataa aatatggcac gaataattgc aattattatg
     1561 gccttatttg gtcataaagg gcattcgtaa tttcggtatt aaaaaagata tctacgttta
     1621 gatatctttt ttatatattg taataccatc tttaattgtc tcaatgatct taatctgacg
     1681 aatttcctcg attgcaactt tgcaaggatt acgatcaagg attaaaagat cagctttttt
     1741 tccttcttta atagaccctt tcaagtgttc ttcaaaatat tgataagcgg cattaattgt
     1801 tactcctttt aatgcgttgt agacagatat ttgttcatct gtccccaaag taaccccgtt
     1861 tttggtttgg cgttttacag cacaccaaat cgtttctaac atgtttggct caataacagg
     1921 ggtatcttga tggaaagtat aaggtaaatc taattttaaa gcactatgaa cacaagaaat
     1981 agattcagca cgttgtccaa gattacggcg gtgaatatca ccccaataat agatatgagc
     2041 aatgaagaaa ctaggaatca ttccaatgct ttttagccgc ggtagttgat ctggtcttaa
     2101 ggtttgacta tgaatcatta cagggcgtag aggtgtgtac tgatcattaa aggcatcaag
     2161 tagttgattg gaagcagcat caccgttaca atgagttagt agttgcgctt tatcattaat
     2221 agccatatct acgaattttt taacatctga atctaagtag atcggatatc cacaatagtc
     2281 accactattt tcatatggct tagataacca ggcggttttt ccttgtggag aaccatctag
     2341 aaataactta taaccgccta gtttaaaatg atttaagtaa tgatgatatt gttgattatc
     2401 ccgatataaa gaataattat ttttaatatc aacataacct acaacatcga gaaataattt
     2461 at
//
LOCUS       DAOMTA010000469         2461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1963_length_2461_cov_2.268911, whole genome shotgun sequence.
ACCESSION   DAOMTA010000469 DAOMTA010000000
VERSION     DAOMTA010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_2461_cov_2.268911"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..670)
                     /locus_tag="PLK68_11565"
     CDS             complement(212..670)
                     /locus_tag="PLK68_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91083.1"
                     /translation="MVFIEENCMTVIADMMSYIFAEIIMIIFMFYLNIWLGILSLLIA
                     GAILWLGKAMEQQTLEHSTIRQEQSEKLTNAVLDFIEGISIIKTYNLLGEKSAELSNN
                     FKESCRTNLEFEETHAPWQRWLNIIYGLGIAAILALSLYLQTQNLLTVPY"
     gene            complement(658..1014)
                     /locus_tag="PLK68_11570"
     CDS             complement(658..1014)
                     /locus_tag="PLK68_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91084.1"
                     /translation="MISLMSRILAIAGKYRRNIKIAFIFSFLKSMLAKSPIGLAFIAF
                     NAFYNNKMSDRLCFLLGITLIVCVLFQAVFQNISDRLQGDSGYRIFSDMRMEWVTLLK
                     EILGKLVQYYLRIWSL"
     gene            complement(1017..>2461)
                     /locus_tag="PLK68_11575"
     CDS             complement(1017..>2461)
                     /locus_tag="PLK68_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91085.1"
                     /translation="QPLGVIQEKGVGAHKKTFIDDIEAIELLLAHALPEGISNLAIPL
                     FIYIVMFIVDWKLALLSLGSLPIGLLAMGAMFKIGMKEMSNYYVAAQKMNNTIIEYVN
                     GMEVVKVFNRDGESYHRFENDIRGYRDFTLAWYKACWPWMALYNSILPCVALLTLPFG
                     SWLVLHNYSSLPDLVLILCLSFAIGTPLLRALAFVSTLPQISYKIESLEKMLSAPPLQ
                     QTSDCFKDRNHHISFDNVSFAYNNNVVLHNINLEIKEGSLTALIGESGSGKSTLAKLL
                     VHFYDVHSGSIKIGNQDLRQMSIETLNNQIAYVSQEQFLFNTALMENIRMGRLDASDE
                     EVYQAASKAQCDEFLSRLELGIHTLAGDGGKQLSGGERQRISLARAILKDAPIIVLDE
                     ATAFMDPENEEKMNAAIAEVIKDKTVIVIAHHLHSVINAHQICVLKNGYLNAVGTHQE
                     LIDTCLEYQKLWTMANNSANWRVSHNQGGN"
BASE COUNT          848 a          450 c          368 g          795 t
ORIGIN      
        1 ttatcataag agaaactaac atttttaaat tttatttctg gtagatcact ttgttctgga
       61 attacttcca taccatcatc aggcagttct ttttgagcta agacatcttc gacacgatcc
      121 atacaactac tcattacagt taaccgtgta ctttgactat acaacgcttt aagtggacca
      181 aataagtcaa atacaaataa agttactcca atcagtaagg aacagttagt aaattttgag
      241 tttgtagata taatgagagt gctaaaatag cagctattcc taaaccataa ataatattta
      301 gccaacgttg ccagggagca tgtgtttctt caaattctaa attagttcgg cagctctctt
      361 taaaattatt agaaagttca gctgattttt cacctaacag attataagtt ttaataatac
      421 taataccctc aataaaatca agtacagcat tagtgagttt ttcactttgt tcttgtctga
      481 tagttgaatg ttctaatgtt tgttgttcca tcgctttacc taaccatagg attgctccag
      541 caattaacaa tgaaagtatc cccaaccaaa tatttaaata gaacataaag atgatcataa
      601 taatttcagc aaaaatatag ctcatcatgt cagcaatcac ggtcatacaa ttttcttcta
      661 taaagaccat atccgtagat aatactgaac taattttccc aatatttcct tcagtaaagt
      721 aacccattcc atgcgcatat ctgaaaaaat tcgataacca gaatcacctt gtaaacgatc
      781 agaaatattt tgaaaaactg cttgaaataa aacacaaact attaatgtaa taccaagcag
      841 aaaacataat cgatcagaca ttttattatt ataaaaagca ttaaaagcaa taaatgctag
      901 tccgattggg gattttgcta acattgattt taaaaacgag aaaataaaag cgattttaat
      961 attgcgacga tattttccgg cgatagcaag gattcgtgac attaatgaga tcataattaa
     1021 ttacctcctt gattatgact aactctccaa ttagcactat tatttgccat ggtccataac
     1081 ttttggtatt caaggcaagt atctataagt tcttgatgag ttcctactgc gtttaaatag
     1141 ccattcttaa gtacacaaat ctgatgtgca ttgataactg aatgtagatg gtgggcaata
     1201 acaataacag ttttatcttt gataacttct gcaattgcgg cattcatttt ttcttcattt
     1261 tctggatcca taaatgcagt agcttcatca agaacaatga ttggagcatc tttgagaatt
     1321 gcccgagcaa gagaaattct ttgtcgttca ccaccggata attgttttcc tccatcacct
     1381 gccaaagtat gaattcctaa ttcaagacgg cttaaaaatt catcacactg tgctttacta
     1441 gctgcttgat aaacttcttc atcactagca tcaagccgtc ccattctaat attttccatc
     1501 aaagctgtat taaatagaaa ttgttcttgt gaaacatagg caatttgatt atttaacgtt
     1561 tcaatgctca tttgtcgtaa atcttgattt cctattttga tcgaacctga atggacatca
     1621 taaaaatgaa caagcagttt tgctaatgtg ctttttccac ttccagattc cccaatcaaa
     1681 gccgttagtg agccttcttt aatttctaag ttaatattat gtaaaactac attattattg
     1741 tatgcaaaac taacattatc aaagctgata tgatggtttc tgtctttaaa acaatcagat
     1801 gtttgttgta gtggcggtgc acttaacatt ttttcaagag actcaatttt ataactgatt
     1861 tgtggtaaag ttgagacaaa cgccaaagcc cgtaaaagcg gagtaccaat tgcgaatgat
     1921 aagcaaagaa taagaactaa gtcaggtaaa ctcgaatagt tgtgaagaac taaccaagat
     1981 ccaaatggta gagtaagtaa agctacacac ggtaaaatac tattatagag tgccatccat
     2041 ggccaacaag ctttgtacca ggcaagagtg aaatcacgat atccacgtat atcattttca
     2101 aaacgatgat atgattcacc atcgcgattg aatactttaa caacttccat tccattaaca
     2161 tattcaataa tagtattatt cattttttgt gcagctacgt agtaattact catttctttc
     2221 ataccaattt tgaacattgc ccccatcgct aataaaccaa tgggaagaga acctaaagat
     2281 aataaggcaa gtttccaatc aacaataaac ataacaatat agatgaagag gggaatagct
     2341 aagttcgaga tgccttccgg caaagcatgt gccaatagta gttcaattgc ttcaatatca
     2401 tcaataaaag tttttttatg agcaccaact cctttttctt gaataactcc taatggttgc
     2461 t
//
LOCUS       DAOMTA010000470         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1984_length_2445_cov_2.030544, whole genome shotgun sequence.
ACCESSION   DAOMTA010000470 DAOMTA010000000
VERSION     DAOMTA010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1984_length_2445_cov_2.030544"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PLK68_11580"
     CDS             complement(<1..150)
                     /locus_tag="PLK68_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRM91086.1"
                     /translation="MKKNIAVYAGTFDPVTNGHLDIIERASRMFDTLYVTICINPNKQ
                     GLFSID"
     gene            complement(155..706)
                     /gene="rsmD"
                     /locus_tag="PLK68_11585"
     CDS             complement(155..706)
                     /gene="rsmD"
                     /locus_tag="PLK68_11585"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRM91087.1"
                     /translation="MRVIAGKYKSRQLKSVKSNLTRPTTDRNKENLFNIIGPYFDGGA
                     VLDLFAGSGGLGIEALSRGYEHLYSVDNQYAAFQVIKENFTMLKLANAHVYKLDYRKA
                     LKRFANEKLKFDLILLDPPYGKGLVDDILEFLIVNEMLNDECMIVVEELKEVEFKPFK
                     ELDLIKKNDYGITALNVFKFMAG"
     gene            complement(715..1062)
                     /locus_tag="PLK68_11590"
     CDS             complement(715..1062)
                     /locus_tag="PLK68_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRM91088.1"
                     /translation="MEEILDKLITEIKLDQRYIDYVEAEKKLHNQEIDSLLHEYQNKL
                     NQYDELKKYNQYIDNQEIKDEIRDLKKKIASNEDILDYYCKYHRLNDFLDEMTKVVFG
                     GISNELDLSPYQL"
     gene            complement(1068..1250)
                     /locus_tag="PLK68_11595"
     CDS             complement(1068..1250)
                     /locus_tag="PLK68_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbE-like family protein"
                     /protein_id="HRM91089.1"
                     /translation="MMNEEIRYYLRYHPNWYIVLSRYPQEYEHLIQEYKDGKNQQFID
                     KIDQVSMLINMVEMMM"
     gene            complement(1240..1545)
                     /gene="ylbD"
                     /locus_tag="PLK68_11600"
     CDS             complement(1240..1545)
                     /gene="ylbD"
                     /locus_tag="PLK68_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein YlbD"
                     /protein_id="HRM91090.1"
                     /translation="MDEFKEFLKTIPSIKQDVLNGRYTWQQLYEIFVMYGKDDKFWLP
                     YKTGNSGFDLNMLLEVIKNIDLNALSSSLGSIEKVLNVASTFLDKKEPAKERKWYDE"
     gene            complement(1578..>1867)
                     /locus_tag="PLK68_11605"
     CDS             complement(1578..>1867)
                     /locus_tag="PLK68_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM91091.1"
                     /translation="LGLIIIPTCIIIFKLLKYSQEIKENKSRIILLGIASYFFLHLLI
                     NLGGISGLIPMTGVPLLLISAGGSSSVTAFVAVGVAQAIIAKHNRQKFDTD"
     assembly_gap    1868..1877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1878..2413)
                     /locus_tag="PLK68_11610"
     CDS             complement(<1878..2413)
                     /locus_tag="PLK68_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM91092.1"
                     /translation="MKVAMILIMSYFLTESDKAFVVKGRFKTQQLKSAFYKEKFLKCV
                     FLPMMLVAIAAGVGIFVQKDFGTTVILVTICFVCFIGTPRQYFKKYKRIVWVFIGVCG
                     VLFLIIGTSVLKGYQLGRISTWLAPLSDPYDTSMQLSNALIAFNNGGLFGVGLGNSTQ
                     KFGYIPESQNDFIGAIIY"
BASE COUNT          859 a          446 c          246 g          884 t
ORIGIN      
        1 atcaattgaa aagagtcctt gtttattagg attaatacaa atcgttacat ataaagtatc
       61 aaacattctg cttgctcgtt caataatatc taaatgtcca tttgttacag gatcaaatgt
      121 tcctgcataa accgcaatat ttttcttcat cgtcctatcc tgccataaat ttaaatacat
      181 ttaaagctgt aatgccataa tcattttttt tgatcaaatc taattcttta aatggtttaa
      241 attctacttc ctttaactct tctactacta tcatacattc atcatttaac atttcattta
      301 caattagaaa ttctaatata tcatctacta atccttttcc ataaggagga tctaataaaa
      361 ttaaatcaaa tttaagtttt tcattagcaa atcgttttaa tgctttacga taatctaatt
      421 tatatacatg agcattagct aatttcaaca ttgtaaaatt ttctttaatt acttgaaatg
      481 ctgcatactg gttatcaaca ctatacaaat gttcataccc tcttgataat gcttcaatac
      541 ctagaccacc actaccagca aataaatcca gtactgctcc accatcaaag tatggtccaa
      601 tgatattaaa cagattttct ttattacgat ctgttgtcgg tcttgtcaaa tttgatttaa
      661 cactttttaa ctgtctactt ttatacttac ctgcaattac tctcataagc cctcttataa
      721 ttgatatggt gataaatcta actcattaga aataccccca aatactactt tagtcatttc
      781 atctaaaaaa tcatttaaac gatgatactt acaataataa tctaaaatat cttcattact
      841 tgcaattttc ttctttaaat cacgaatttc atctttaatc tcctgattat caatatattg
      901 attatatttc tttaattcat catattgatt cagtttattt tgatattcat gcaataatga
      961 atcaatctcc tgattatgaa gtttcttttc agcttcaaca taatcaatat atcgctgatc
     1021 cagttttatt tctgtaatta atttatctaa tatctcttcc atcttaccta catcatcatt
     1081 tctaccatat taattaacat ggatacttga tcaatcttgt caataaattg ttgattttta
     1141 ccatctttat attcttgaat taaatgctcg tattcttgag gataccgaga taaaactata
     1201 taccaattag gatgataacg aagataatat ctaatttctt cattcatcat accattttct
     1261 ttccttagca ggctcttttt tatctaaaaa agtactagca acatttaaaa ctttttcaat
     1321 tgaacctaaa ctactcgata aagcatttaa atcaatattc ttaattactt ctagtaacat
     1381 atttaaatca aaaccactat tacctgtttt atatggtaac caaaatttat catctttacc
     1441 atacataaca aaaatttcat ataactgttg ccaagtataa cgtccattaa gaacatcttg
     1501 ttttatacta gggattgttt ttaagaactc tttaaattca tccataagat cacctaatat
     1561 attatatgta tattagccta atcggtgtca aatttttgac gattatgttt tgcaattatt
     1621 gcttgtgcga caccaactgc tacaaatgca gtaactgatg aagatccacc agcagaaatt
     1681 aacagtaatg gtactccggt cataggaatt aatccagata ttccaccaag gttaatcaat
     1741 aaatgtaaaa agaaatatga agcaattccc aacaaaataa tccgtgattt attttcttta
     1801 atttcttgtg aatattttaa taacttaaaa ataatgatac atgttggaat aattattaag
     1861 cctaaacnnn nnnnnnntca tatataatag caccaataaa atcgttttgt gactctggaa
     1921 tatatccaaa tttttgggtt gaatttccta aaccaacacc aaaaagacca ccattattaa
     1981 aagcaattaa agcatttgat aattgcatac ttgtatcata aggatcactt aatggtgcta
     2041 accatgtaga aattctccct aattggtaac cttttaatac tgaagtccca ataattaaaa
     2101 acaatactcc acaaacccca atgaacaccc atacgattcg cttgtatttc ttaaagtatt
     2161 gacgcggtgt cccaataaaa caaacaaaac aaatagttac taaaataaca gttgttccaa
     2221 aatctttttg cacaaagatc ccaactccag cagcaattgc tactaacatc attggcagga
     2281 aaacacattt taaaaacttt tctttataaa aagcagattt cagctgttgt gttttaaatc
     2341 taccttttac tacaaaggct ttatcacttt cagttaaaaa ataactcatt atcagtatca
     2401 ttgcaacttt catgaactca gctggctgaa tactaaatag agaac
//
LOCUS       DAOMTA010000471         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_1985_length_2445_cov_1.862343, whole genome shotgun sequence.
ACCESSION   DAOMTA010000471 DAOMTA010000000
VERSION     DAOMTA010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_2445_cov_1.862343"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLK68_11615"
     CDS             complement(<1..266)
                     /locus_tag="PLK68_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91093.1"
                     /translation="MLKGDGDTYIYIIDKNYKILYCNDNALQKFPCVKVGELCYHAVR
                     DEKKPCHDCPLQLNSNSTRPIFYNRKINQWQEINSAIIDWLNYK"
     gene            437..>629
                     /locus_tag="PLK68_11620"
     CDS             437..>629
                     /locus_tag="PLK68_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91094.1"
                     /translation="MLIIEKLNLKEKMSDGEESIAAFILTLGKELHKYSTRNIAEATL
                     TSPATVIRLCKKLDFKGFDD"
     assembly_gap    630..652
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <653..1372
                     /locus_tag="PLK68_11625"
     CDS             <653..1372
                     /locus_tag="PLK68_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM91095.1"
                     /translation="QFLKEMNYLDQQYGKVDANFPFNRNDTMMKTAHKISHLYEDTVT
                     DTMTLLHHDDLQKALRLLKNSNSIHIFSTGTALNLAESFKEKMLKIGKNVVISNNLNY
                     QLYEVSCIPKGDIAIIISYSGETINTIKIAQTCKNNKIPIIAITSFGENTLAKLASCK
                     LTISTKESLYHNLGDFSTHLATHLMLDILYSVFFLEDYDQNYDNKIKITKDLEALRSS
                     TNPIINDGMKHCFMNFNIEMI"
     gene            1457..>2445
                     /locus_tag="PLK68_11630"
     CDS             1457..>2445
                     /locus_tag="PLK68_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91096.1"
                     /translation="MKIKDNFLWGGATAANQYEGAYREDGKGLSIADVEMGSSHGVPR
                     EIHDRVQSGCYYPSHEGIDFYHHYQEDIALFAKMGFKCFRMSINWPRIFPNGDEYEPN
                     QAGLEFYDRVFAELAKYQIEPIVTLSHYETPLYLVQKYGSWRNRKLIDFFERFCEVIF
                     TRYREQVRYWMTFNEINEVMNQEMPYHQAGIIYQPGENHGDVKLQVSHHLMIASAKAV
                     ILGHRINPEFKIGCMLQYPMTYGATCRPQDELAKRLSMLPNFYYSDVMVRGHYTNTCR
                     AQAKRLNGNFITIKGDEEILQAGKVDYIAISYYFSSIASYSKDSEIKVTRDNP"
BASE COUNT          835 a          324 c          423 g          840 t
ORIGIN      
        1 tctttataat ttaaccaatc aataatagca ctattgattt cctgccattg atttattttg
       61 cgattataaa aaataggtct tgtactatta ctatttagct gtaatggaca atcatgacaa
      121 ggcttttttt cgtctctaac tgcatgatag cataattcac caactttaac acatgggaat
      181 ttttgtagag cattatcatt acaatacaaa attttataat ttttatcaat tatgtaaata
      241 taggtatcac catcaccttt taacatgaaa tcactccttt tacccttagt cttataatat
      301 tattataggt gatttgtttt atcatagcaa taaacgcaat aatttaaatt ttagacatca
      361 gattttatga ttattataaa tttcgaaaaa atatatggtt taaatatgat aggattagaa
      421 aaaggtgatg attgaaatgt taataattga aaaattaaac ttaaaagaaa agatgtctga
      481 tggtgaagaa agtattgcag cttttatctt gaccttaggt aaggaacttc ataaatattc
      541 aactcgaaat attgccgaag caactttaac ctcaccagct actgtaattc gtttgtgtaa
      601 gaaacttgat tttaaaggtt ttgatgattn nnnnnnnnnn nnnnnnnnnn nncaattttt
      661 aaaagaaatg aattatcttg atcaacaata tggaaaggtt gatgctaatt ttccttttaa
      721 tcgtaatgat acgatgatga agactgctca taagatatca catttatatg aagatacagt
      781 aacagatact atgacattgt tgcatcatga tgatcttcaa aaagctttac ggttattaaa
      841 aaatagtaat tctattcata ttttttctac aggaacagct ttaaatttag ctgagtcttt
      901 taaagaaaag atgttgaaaa ttggtaagaa tgtagtaatt tcaaataatt taaattatca
      961 attgtatgag gttagctgta ttccaaaagg agatatcgcc attattattt cctactcggg
     1021 tgaaacgatc aatacaatta aaattgctca aacttgtaaa aataataaga ttccgattat
     1081 tgcaatcact tcatttggtg aaaatactct tgctaagtta gcatcatgta aattgaccat
     1141 ttcaacaaaa gaaagcttat atcataatct tggggatttt agtacccatc ttgcgacaca
     1201 tttgatgttg gatattttgt attcagtttt ctttcttgaa gactatgatc aaaattatga
     1261 taataaaatt aagattacaa aagatttaga agcgttgcgt agttcgacta atcccatcat
     1321 taatgatggg atgaaacact gtttcatgaa ttttaatatt gagatgatct aaatttaaac
     1381 agtgttaaaa tctctttaat ttctatatag taatgacctc cttattggta tattaagcta
     1441 gtaaggaggt tttttaatga aaataaagga taatttttta tggggtggtg ctactgccgc
     1501 aaatcaatat gagggagctt accgtgagga tggtaaggga ctaagtatag ctgatgtcga
     1561 aatgggatcg agtcacggag ttccccgtga aattcatgat agggttcaat caggctgtta
     1621 ttatcctagt catgaaggaa ttgattttta tcatcattat caagaggata ttgccttgtt
     1681 tgctaaaatg ggatttaagt gttttcgaat gtcgatcaat tggccccgga tttttcctaa
     1741 tggggatgag tacgaaccta atcaggctgg tttagaattt tatgatcgtg tttttgctga
     1801 actagctaaa tatcaaattg agccaatcgt tacattatca cattatgaaa cacctctcta
     1861 tcttgttcaa aaatatggat catggcgtaa tcgaaagtta attgattttt ttgaacgctt
     1921 ttgtgaagtt atatttacca gatatcgaga gcaagtaaga tactggatga cattcaatga
     1981 aattaatgag gtaatgaatc aagagatgcc ttatcatcaa gcaggaatca tttatcagcc
     2041 gggagaaaat catggtgatg taaagttaca ggtttctcat catttgatga ttgcaagtgc
     2101 gaaagcagta attttaggac atcgaattaa tccggaattt aaaattggat gtatgttgca
     2161 gtatccaatg acttatggag caacgtgtag accgcaagac gaattagcaa aacgtctttc
     2221 gatgctgcct aatttttatt atagtgacgt aatggttagg gggcattata ctaatacttg
     2281 ccgagctcaa gcaaagagat taaatggtaa ttttataacg ataaaagggg atgaagaaat
     2341 tttacaggct ggtaaagtgg attatattgc aattagttat tatttttcat caattgcttc
     2401 gtattcaaaa gattcagaaa ttaaagtcac aagggataat cccta
//
LOCUS       DAOMTA010000472         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2002_length_2432_cov_1.832562, whole genome shotgun sequence.
ACCESSION   DAOMTA010000472 DAOMTA010000000
VERSION     DAOMTA010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2002_length_2432_cov_1.832562"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..521)
                     /gene="rplT"
                     /locus_tag="PLK68_11635"
     CDS             complement(162..521)
                     /gene="rplT"
                     /locus_tag="PLK68_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRM91097.1"
                     /translation="MARVKGGFTTRRRRKKVLKLAKGYFGSKHTLYKTAHEQVMHSLA
                     YSYRDRRQVKRDMRKLWIARINAAARLNDISYSKLMHGLKLANVEVNRKMLSEIAICD
                     PKGFTAIVNTAKKALAK"
     gene            complement(547..741)
                     /gene="rpmI"
                     /locus_tag="PLK68_11640"
     CDS             complement(547..741)
                     /gene="rpmI"
                     /locus_tag="PLK68_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRM91098.1"
                     /translation="MPKMKSHSGLKKRLKRTGSGKLKRSHAYVSHLSHNKTHKQKKHL
                     AKATLVSASDYKRIKSRLAK"
     gene            complement(759..1313)
                     /gene="infC"
                     /locus_tag="PLK68_11645"
     CDS             complement(759..1313)
                     /gene="infC"
                     /locus_tag="PLK68_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRM91099.1"
                     /translation="MEVAFISRFDNRKPKQPDELVNEKIRFKEVLVIDQNGDQLGVKS
                     RNQAIDIAYGAGLDLVCVAPNGKPPVCRIMDFGKYRFEQQKKAKEMKRNSKVVALKET
                     QLSVTIDVHDKNVKLKRTLKWLEEGNKVKIAIRFRGRQLAHMDLGKKVIDDFVAECAE
                     VGQIEKPAKLEGRTLTAIIAPKKK"
     misc_feature    complement(1320..1441)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(1873..>2432)
                     /locus_tag="PLK68_11650"
     CDS             complement(1873..>2432)
                     /locus_tag="PLK68_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRM91100.1"
                     /translation="KTAYHLVGEYLKSKNKNARVFIVHRLDKETSGVLLFAKNEIIKN
                     KLQNNWNDIVYKRGYLAIIEGKLKKKHGTIKNHLDESKTQMVYIANNGKGKLAITNYK
                     VLKESRYNSLIEVFLETGRKNQIRVHMQSLGHSIVGDKKYGATTNPIKRMGLHSHVFA
                     FVHPDTKARMEFKAVVPEEFKKMFK"
BASE COUNT          730 a          465 c          280 g          957 t
ORIGIN      
        1 cactacgcca tgaacaatcc tttaaattag ttattacttt aaaaactgct gctacttcac
       61 ttttaaaatc aactgtcata tctattcttt tcattttttg agccatccac gattccatta
      121 taacacaata aaaaaagaga aagcgaactt tctctaaata attattttgc taatgctttt
      181 tttgctgtat ttacaattgc agtaaatcct ttaggatcac aaattgcgat ttcagataac
      241 attttacggt taacttcaac gttagctaat tttaatccgt gcattaattt tgaatatgag
      301 atatcattta atcttgcagc agcattgata cgtgcaatcc ataattttct catatctctt
      361 tttacttgtc ttctatctct atatgaatat gctaatgaat gcataacttg ttcatgagca
      421 gttttatata atgtatgttt tgatccaaaa tatcctttgg ctaattttaa tacttttttt
      481 cttctacgtc ttgtagtaaa tccacctttt actcttgcca tttgtttctt cctcctatgt
      541 caataattat tttgctaatc tagatttaat tctcttgtag tctgatgcac ttactaaagt
      601 agctttagct aagtgtttct tttgtttatg agttttattg tgagataaat gtgatacata
      661 agcatggctg cgttttaatt taccgctacc tgttcttttt aaacgttttt taagaccact
      721 atgtgatttc atttttggca tgatatattc ctcctaaatt attttttctt tggtgcaatt
      781 attgcagtta gagttctacc ttctagctta gctggttttt caatttgacc aacttcagca
      841 cattcagcta cgaagtcatc aataactttt ttcccaagat ccatatgagc taattgtctt
      901 cctctaaatc taattgcaat tttaactttg ttaccttctt ctaaccattt caatgttctt
      961 tttaatttta catttttatc atgaacatca atagttaccg ataattgagt ttcctttaaa
     1021 gcaaccactt tagaattacg tttcatttct ttagctttct tttgttgttc gaaacgatat
     1081 ttaccaaagt ccataatacg acatactggt ggtttaccgt taggggcaac acacactaaa
     1141 tcaagtcctg caccataagc aatatcaatt gcctggttac gacttttgac acctaattga
     1201 tcaccatttt gatcaatgac tagtacttct ttgaaacgaa ttttttcgtt tactaactca
     1261 tccggttgtt ttggttttct gttatcaaat ctgctaataa aagccacctc cattttagta
     1321 gtttaaacaa aaagtgagca cactgagtac ccacacattg acattaagat agtttatctt
     1381 agcgacattt gcctatcgac ataatcaatc aggcgagaag taggcacttc ttctttccac
     1441 tacttttcat ttacttgagt atattatcat tttatttaat taaagtcaac ccatatttaa
     1501 aagatttatt catcttcatc tatttgtaaa tatttaaagt atccaaacac cgaaaagact
     1561 atcaatacta ttaatgcaaa catttcatca ccactaataa tatatgtaat cccaataaaa
     1621 ttattacttg tttttatgat tatgtttaaa tactttccat tctacagcaa ttatatttat
     1681 taataaatct aaacttagcc aaattcttaa tattttcact atcaagatac caccatttat
     1741 ttctcactta gcgaccgctt gccccctagt tcttcttgtg tcaaattata ctgcgcccac
     1801 agcctcttgc tactatagca tatcgactaa atagaagcta gaaaatgcct tgctacttat
     1861 cataacaagg cattatttaa acattttttt aaattcttct ggtacaaccg ctttaaattc
     1921 cattctagct tttgtatccg gatgaacaaa ggcgaataca tgactatgaa gtcccattcg
     1981 tttaatcgga ttagttgtag caccatactt tttatcccca acaatactat gacctaatga
     2041 ttgcatatgg acccgaatct gatttttacg acccgtttca agaaatactt caatcaatga
     2101 attatatcgt gattctttta atactttata atttgtaatc gccaatttac ctttaccatt
     2161 attagcaata taaaccattt gtgtcttact ttcatcaagg tgatttttaa tcgttccatg
     2221 tttcttttta agctttcctt caataattgc caaataacca cgcttataaa caatatcatt
     2281 ccaattattt tgtaatttat ttttaattat ttcattttta gcaaacaaca gtacccccga
     2341 tgtttcttta tcaagtcgat gaacaataaa aacacgggca tttttatttt tacttttaag
     2401 atactctcca actaaatgat aagctgtttt tt
//
LOCUS       DAOMTA010000473         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2004_length_2431_cov_2.034933, whole genome shotgun sequence.
ACCESSION   DAOMTA010000473 DAOMTA010000000
VERSION     DAOMTA010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_2431_cov_2.034933"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLK68_11655"
     CDS             <1..363
                     /locus_tag="PLK68_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRM91101.1"
                     /translation="TVVASKILELVPSVTEEMKALSDGFDVFTNIINDGINYDSFDAR
                     NVAALAASLNSAYKHGLINQEDLESALIKYYDKIDRVNEPISLNQDQYNEVVDVLSKL
                     DSAKFDQTKILNKIIVSE"
     gene            365..>2431
                     /locus_tag="PLK68_11660"
     CDS             365..>2431
                     /locus_tag="PLK68_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRM91102.1"
                     /translation="MKNIYDTLEFNQIKNKISTYCVSSLGKTRIAELMPLKDTDDLKL
                     EQKYLDQAMKLIFKYGKMPIGHFIDIEPLLLKTTKDGTLFGEDFVQIVYLLNNVKEVQ
                     SYLSEKELVDNELLQLCNALVLPKQLLADINRCIDSSGNVLDGASSELRRIRRQILSI
                     ESNIRTKIDQVKVANKDYLSQEAISSRNNHLVLPVKAGNKNLIKGIVHAVSSSGQTMF
                     IEPDIIVQMNNQLVHAKDDERREVNRILTVLSNNVKDHYDVLHEDQELMIDIDVIFAK
                     AAYGVKIDGIVPEVAEDYQHFSLIRARHPLIEPHDVVANDIVLNAPKRMLLISGSNTG
                     GKTVVLKTAGLLSVMALCAMALPCTKATVPMFDQICVDLGDEQSIEQSLSTFSSHMKR
                     LVEITNEVTEKSLVLLDEIGSGTDPREGQSIAEAILRYLHQINPLIVASTHYSGLKEF
                     AKNEDYILVAAVEFDQELMQPTYRLIEGSVGNSYAIEISSRLGLNQEIVDMAYQIKEE
                     SLTDTDKLLEKLQNELAQVQIEKDRLEALTNETEVKMKKYERLISSFEKHKDEMMEKA
                     KAEANKLLEESKQEIDLVVEDLKQQAVLKQHVVIDAKRNLDLLKHEPKKVINEEVHEY
                     QVGDVVKVLSANRQGEILSINKKGILTINMSGLKLNAKPEEVSFISKKVKPKKVKSNL
                     KSLRKTT"
BASE COUNT          924 a          301 c          461 g          745 t
ORIGIN      
        1 acagttgtag caagcaaaat tttagagctt gtaccttcag ttactgaaga aatgaaagca
       61 ttatcagacg ggtttgatgt ttttactaat attattaatg atgggataaa ttatgatagt
      121 tttgatgccc gaaacgttgc tgcgcttgct gcttcattga acagtgctta taaacatggt
      181 ttaattaatc aggaagacct tgaatcagcg ttaataaaat attatgataa aatcgatcgg
      241 gttaatgagc ctatttcgtt aaatcaagat caatataatg aggttgttga tgtattatca
      301 aaattagatt cagctaaatt tgatcaaaca aaaattttaa ataagataat agtgagtgaa
      361 tagaatgaaa aatatatatg atacattaga atttaatcaa attaaaaata aaatcagtac
      421 ttattgtgtt agttcattag ggaaaacaag gatagctgag ttaatgccgt taaaagatac
      481 tgatgatttg aagctggaac aaaaatattt agatcaagca atgaaactga tttttaaata
      541 cgggaaaatg ccaatcggtc attttattga tattgaaccg ttattattaa aaacaacaaa
      601 agatggtact ttattcggtg aagattttgt ccaaattgtt tatttgttga ataatgtcaa
      661 agaagtacaa agctatttga gcgaaaaaga attggtagac aatgagctgt tgcaattatg
      721 caatgcgcta gttttaccta aacaattatt agctgatatt aatcgctgta ttgatagtag
      781 tggaaatgtt ttagatggtg ctagttcgga attaagacga attagacgcc aaattttatc
      841 aattgaatct aatattagaa caaaaattga tcaagtaaaa gttgctaata aagattattt
      901 aagtcaagaa gctatttctt caagaaataa tcatctggtt ttgcctgtca aagccggcaa
      961 taaaaattta attaaaggaa ttgtccatgc agtttcatca agcggacaaa caatgtttat
     1021 tgaacctgat ataattgttc aaatgaataa tcagctagtt catgctaaag atgatgaacg
     1081 acgtgaagta aatcgaatct taaccgtact ttcgaataat gtaaaagacc attatgatgt
     1141 tctacatgaa gaccaagaac taatgattga tattgatgtt attttcgcaa aggctgctta
     1201 tggagtcaaa atagatggaa tagttccaga agtggctgaa gactaccagc attttagctt
     1261 gattagagcg agacatcctt taattgagcc acatgatgtt gtagctaatg atatagtgtt
     1321 aaatgcacca aagagaatgt tattgattag tggatcgaat acaggaggga aaacagttgt
     1381 tttaaaaacg gctgggttat taagtgtgat ggcactgtgt gctatggctt tgccttgtac
     1441 taaagcaacg gttccaatgt ttgatcaaat ttgtgttgat ctcggtgatg aacaatcaat
     1501 tgaacaatca ttgtcaactt tttctagcca tatgaaaaga ttagttgaaa taacaaatga
     1561 agtaactgaa aaatcattag ttttactaga tgaaattggt tctggaacag atccgcgtga
     1621 aggacaaagt attgctgaag caatcttaag atatttacat caaatcaatc ccttgattgt
     1681 agcttcaacc cattattcag gcttaaaaga atttgctaaa aatgaagatt atattttagt
     1741 agcagcagta gaatttgatc aagaactgat gcagccaact tatcgcttga ttgagggcag
     1801 tgttgggaat tcttatgcga tcgaaatttc atcgcgatta ggtcttaatc aagaaattgt
     1861 tgatatggct tatcaaatca aagaagaaag tctgacagat acagataaat tattagaaaa
     1921 actgcaaaat gaactagctc aggttcaaat tgaaaaagat cgtttagaag ctttaacaaa
     1981 cgaaacagaa gttaagatga agaaatatga acgcttaatt agcagttttg aaaaacataa
     2041 agatgaaatg atggaaaagg caaaagcgga agctaataaa ctacttgagg aatcgaagca
     2101 ggaaattgat ttagtggtag aagatttaaa acaacaagct gttttaaaac aacatgttgt
     2161 cattgatgct aagagaaatc ttgatttatt aaaacatgaa ccaaaaaaag ttattaatga
     2221 agaagtccat gaatatcaag ttggtgatgt agtcaaagta ttatcagcta atcgtcaggg
     2281 ggaaattctt tcaattaata aaaagggaat tttaactatt aatatgagtg gattaaaatt
     2341 aaatgcaaag cccgaggaag ttagctttat ttctaagaaa gtgaagccta aaaaagttaa
     2401 atcaaatcta aaatctttgc gaaaaacaac a
//
LOCUS       DAOMTA010000474         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2012_length_2426_cov_2.344580, whole genome shotgun sequence.
ACCESSION   DAOMTA010000474 DAOMTA010000000
VERSION     DAOMTA010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_2426_cov_2.344580"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLK68_11665"
     CDS             <1..474
                     /locus_tag="PLK68_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRM91103.1"
                     /translation="NLYEIEVLDNNNQTISLNNYKDQVLLIVNTATDCEFTEQYDDLE
                     ELYEKYHNYGFCILDFPCNQFGEQAPGTMEEIMSFCADTYGVSFPQFAKIEVNGPNES
                     PLYTYLKEKQDSTLSKRIDWNFTKFLVNQEGDVVARFEPLVKPREIENNIKKLLK"
     gene            663..1157
                     /locus_tag="PLK68_11670"
     CDS             663..1157
                     /locus_tag="PLK68_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRM91104.1"
                     /translation="MVKFAIYTFLGFILESVYVSILKKEFYLSGLLKGPFIPIYGFGA
                     LLILSIVPYHTNNFEIFFYSLFGCTALEYLTHFFLSYDSQIEVWNYGKIPSNYTSRIC
                     MFYSLMWGFLGIILVNYIDPFINSLLINLNYYAVNIIALIYILIILYQFYNQQFQVTK
                     KQPK"
     gene            complement(1170..1478)
                     /locus_tag="PLK68_11675"
     CDS             complement(1170..1478)
                     /locus_tag="PLK68_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91105.1"
                     /translation="MKKIVLLWDTEIPSDFFVEKLIEESEKGSVKCEIQAYSIAEALE
                     NRIDADVVLLSPLICFEQIKIERLVRCPVDIIGIGAYALFDVKAILLKAFDAMRKKRI
                     "
     gene            complement(1760..>2426)
                     /locus_tag="PLK68_11680"
     CDS             complement(1760..>2426)
                     /locus_tag="PLK68_11680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM91106.1"
                     /translation="HYFYPKNDVAVSKDLPLKNVSVVIDPGHGGLDNGASVGKIYESE
                     LNLKISYALKEELESRGATVNMTREDEQDMTKRNHHYSKQDDMYLRVKKIDSYKSDYL
                     ISIHLNSAPASGAWGSQVFYYKNSDKGKRLASEIQTTMKEVTGSAKRISGADFRVLRA
                     TQTVGVLIECGFISNANERGQLQSSKYHQKLAVKICDGIEKYREKYPEDTIDPKDYEK
                     ILS"
BASE COUNT          817 a          406 c          295 g          908 t
ORIGIN      
        1 aacttatatg aaatagaagt attagataat aacaatcaaa caatctcact aaataattat
       61 aaagatcaag ttttactaat cgtcaataca gcaaccgatt gtgaattcac cgagcaatat
      121 gatgatttag aagaacttta tgaaaaatat cataattatg gtttttgtat tctagatttc
      181 ccatgtaatc aattcggtga acaagcacct ggaacaatgg aagaaatcat gtctttttgt
      241 gcggatactt atggtgttag ttttccgcaa tttgctaaaa tcgaagtcaa cggcccaaat
      301 gaatcaccac tttatacata tttaaaagaa aaacaagata gtactttatc aaaacgaatc
      361 gactggaact tcacaaaatt tttagttaat caagagggtg atgtcgttgc ccgctttgaa
      421 ccgttagtaa agccaagaga aattgaaaat aatattaaaa aattattaaa ataaatcttt
      481 ataacaacat acttgcattg caatataata aaaactacta aattgaatga acttattatt
      541 ttaaatcaaa aataataagt tcatttttaa tattaatatt catcgtatct gatacctcat
      601 ctttaagata agattcttgt atattcctct tttaattaca tatattatta aaggaggtaa
      661 taatggtaaa atttgcaatt tatacatttt taggatttat tcttgagtca gtatatgttt
      721 caatcttaaa gaaagaattc tatctatcgg gattactaaa aggaccgttt atccctatct
      781 atggctttgg tgcattattg attttaagca ttgtccctta tcatactaat aattttgaaa
      841 tcttttttta ttcactattc ggctgtactg ccctagaata tttgactcac tttttcttat
      901 catatgattc acaaatagaa gtatggaatt atggtaaaat acctagcaat tatactagcc
      961 gtatttgtat gttctatagc ttgatgtggg gatttttagg aattatttta gttaattaca
     1021 ttgatccatt tattaatagc cttctaatta atttaaatta ttatgctgtt aatattatcg
     1081 cactcatata tatccttata atcctatatc aattttataa tcagcaattt caagtaacaa
     1141 aaaagcagcc taaataggtt gcctacatat tagattctct tttttctcat cgcatcaaat
     1201 gcttttaata aaattgcctt aacatcaaat aaagcataag caccaattcc gattatatca
     1261 accggacaac gtactaaacg ctcaatttta atttgttcaa aacaaatcaa aggacttaac
     1321 aagacaacat cagcatcaat tcggttttct aatgcttcag caattgaata agcttgaatt
     1381 tcacatttta cagagccttt ttcagattct tcaatcagct tttcaacaaa aaaatcactt
     1441 ggtatttctg tatcccacaa taaaacaatt tttttcatct tgctaaaccc ctttctttta
     1501 tattttggta taatttgttg tattttatcc attaatttat cacattaaaa actttttata
     1561 agcaaaaatt attttttata cacacaaata tatcatttac aagaaaagat gtaactgctt
     1621 tcattactat ttattatatt tttttattgt ttttgacaat atattttatt atttatattt
     1681 aaaaaactcc tataaaatga agcacacttc cataactcag ccaactaaat tatagagtgt
     1741 acttaaaaaa ggagtttttc tacgataata tcttttcata atcctttgga tcaattgtat
     1801 cttctggata tttctcacga tatttttcaa tcccatcaca tattttgact gctaattttt
     1861 gatgatactt actactttgc agttgtcctc tttcattagc attagagata aaaccacatt
     1921 caatcaatac tccgactgtc tgggttgccc gtaaaactcg gaaatctgca cctgaaatgc
     1981 gttttgcact tcctgtaact tctttcatcg tagtttgtat ttcactggca aggcgctttc
     2041 ctttatcact gtttttataa taaaatactt gggaacccca agcacctgag gctggagctg
     2101 agtttaaatg aatactgatt aaataatcac ttttatatga atctatcttt ttaaccctta
     2161 aatacatatc atcctgtttt gaatagtgat gatttcgttt ggtcatatcc tgttcgtctt
     2221 cacgagtcat atttactgtt gcccctcgac tttcaagttc ttctttcaag gcatatgaaa
     2281 tttttaaatt taattccgat tcataaattt ttccaacact tgcaccatta tcaaggcccc
     2341 catgtccggg atcaattact actgatacat tttttaatgg taaatctttg cttacggcca
     2401 catcattttt aggataaaaa taatgt
//
LOCUS       DAOMTA010000475         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2025_length_2418_cov_2.074482, whole genome shotgun sequence.
ACCESSION   DAOMTA010000475 DAOMTA010000000
VERSION     DAOMTA010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2025_length_2418_cov_2.074482"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLK68_11685"
     CDS             <1..662
                     /locus_tag="PLK68_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM91107.1"
                     /translation="IGAGGGFVEIGDRMLYHKQVSRTAINHVVDYFEANEFDYYIESN
                     GGLYASKNLIPRLERIMYGDLENDPVARRTKAETPNHFIGSLKEGYDLHRDDVNKICF
                     LEKDNFPFEKIKKEFEQEFNVIHCTVPIFGDNSGELSVPGVNKASAINALIDELGIPK
                     ENTYAFGDGLNDADMLEFCQYGIAVGNAKEALKEIADEVTDDIKDDGIYNSMKKYGLI
                     D"
     gene            757..1431
                     /locus_tag="PLK68_11690"
     CDS             757..1431
                     /locus_tag="PLK68_11690"
                     /inference="COORDINATES: protein motif:HMM:NF036873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91108.1"
                     /translation="MESKNNNLKDLIVSGSYTAFIKGDDWGCGVNKILLSLDHKIDQV
                     NNLSFVVKEKKLTTDYCDTLYPIIESIIYRTVTNVYLVDYSGQITSEPSNFIMIEMKI
                     SPAEGNPLLFSMQTQYNTYNDLYELDIMIADGHEMTSLGQKVKQINIAKKMKDKITSA
                     DLFEEALYESCDGVSYKYAAYNPVQASDTLVVWLHGLGEGGTINTDPYITVLANKVIS
                     LAGQEF"
     gene            1483..2028
                     /locus_tag="PLK68_11695"
     CDS             1483..2028
                     /locus_tag="PLK68_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91109.1"
                     /translation="MDKTGDSLATKFTEADGTSYYCDSLHELINFYRKETKSEKVIIT
                     GCSNGGYMTMLMALKYGKEYNAYVPICEAFLNEKISDEEIKQLKKLPIFFIYSRDDDV
                     VAPETHELPTIRRLLGAGASNVHTFVSKHVIDTSGRFNDKDGEPLRYSGHWSWIYFFN
                     NEAVSDRCGIRVWQWMAQQLK"
BASE COUNT          851 a          249 c          479 g          839 t
ORIGIN      
        1 ttattggagc tggtggtggt tttgttgaaa ttggagatcg aatgttatat cacaaacaag
       61 tttctcgcac agcaatcaac catgttgttg attattttga ggctaatgaa tttgattatt
      121 acattgaaag taatggtgga ttatatgcaa gtaagaattt aattccacga ttagaaagaa
      181 taatgtatgg ggatttggaa aatgatccag ttgcacgtag aactaaagct gaaactccta
      241 atcattttat tggatcatta aaagaaggtt atgatcttca tcgtgatgat gttaataaga
      301 tttgtttttt ggaaaaagat aatttccctt ttgaaaaaat aaaaaaagaa tttgagcaag
      361 agtttaatgt aattcattgc actgttccta tttttggtga taacagtgga gagttatcgg
      421 ttcctggagt taacaaagct tcagctatta atgctttgat tgatgagtta ggtatcccta
      481 aagaaaatac atatgctttt ggtgacggat taaatgatgc agatatgctg gaattttgtc
      541 aatatgggat tgcggtaggt aatgctaaag aggcattaaa agaaattgct gatgaagtga
      601 cagacgatat taaagatgat ggtatttata attcaatgaa aaaatatggt ttaattgact
      661 aaaaagtcct acgttatgta ggatttttat tttaaagact ctaaatgaat aacttaaata
      721 tgttataata aaggagttat taaagggaga aaatgtatgg aaagtaaaaa taataattta
      781 aaagatttga ttgtatctgg aagttataca gcttttatta aaggtgatga ctggggatgc
      841 ggtgtcaata aaatattgct aagtctcgat cataaaattg atcaggttaa taatctgagt
      901 tttgtggtta aagagaagaa gttgactaca gattattgtg atacgctata tccgattatt
      961 gaaagtatta tttatagaac tgtaacaaat gtttatttag tagactatag tggtcaaata
     1021 actagtgaac catctaattt tattatgata gaaatgaaaa tcagtccagc agaaggaaat
     1081 ccattattgt tttcaatgca gacacaatat aatacatata atgatttata tgaattggac
     1141 atcatgatag ctgatggaca tgaaatgacc tcattaggtc aaaaagttaa acaaataaat
     1201 attgctaaaa aaatgaaaga taagattact agtgcagatc tgtttgaaga agcgttatat
     1261 gaatcttgtg atggagtgag ttataaatat gctgcttata atcctgtgca agcaagtgat
     1321 actttggttg tttggttaca tgggctaggt gagggaggaa caattaatac tgatccttat
     1381 attactgtat tagctaacaa ggttatttca ttagcgggtc aagagttcta aaatacgatt
     1441 ggtggggcta atattctggt acctcagtgt ccaacttttt ggatggataa aactggtgat
     1501 agtttagcaa ctaaatttac ggaagcagat gggacttcat attattgtga ttctttacat
     1561 gaattgatta atttttatcg aaaagaaact aaaagtgaga aagttattat cacaggatgt
     1621 tcaaatggtg gctatatgac aatgttgatg gcacttaagt atggtaaaga atataatgcc
     1681 tatgtgccaa tttgtgaagc ttttttaaat gaaaaaatat ctgatgaaga aattaaacaa
     1741 ttaaaaaaat tgccaatatt ctttatttat tccagagatg atgatgtcgt agcccctgaa
     1801 acccatgaat tgccaacgat taggcgcttg ttaggggctg gggccagcaa cgttcatact
     1861 tttgtttcaa agcatgttat tgatacttct gggcgtttta atgataaaga tggagagcca
     1921 ttacgatata gtggtcattg gtcatggatt tacttcttta ataatgaggc agtatctgat
     1981 cgttgtggaa ttagagtttg gcaatggatg gcacaacagt taaaataatc tgtaaaggta
     2041 aaaaagatag tatctctaaa aatggatgca ttaattcaaa taaaagggtg ttgtccatat
     2101 ttaattagat acattcattt ttttagtaga ttaatttttt tgttactctg ataaagacat
     2161 atttattgat tattacttta agtttattcg ttgaatcgtt tattataact atcgcatttt
     2221 acgtttttta ttatatgaaa agatttatta gtaatgttta caaaagaata ttttgttaaa
     2281 aattagggta atactttata ttttattgta tataactaaa aaattattaa agatagataa
     2341 ggaggataaa gatgaaaaag aagtatagat tattgacatg tttattggtt tttatgttgg
     2401 ggttaactgg atgtacta
//
LOCUS       DAOMTA010000476         2409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2034_length_2409_cov_2.182243, whole genome shotgun sequence.
ACCESSION   DAOMTA010000476 DAOMTA010000000
VERSION     DAOMTA010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_2409_cov_2.182243"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLK68_11700"
     CDS             complement(<1..456)
                     /locus_tag="PLK68_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRM91110.1"
                     /translation="MKLLIYLLGIIGSVGSLVYSFNFSSNNHFIFISCIIVGLLIYLG
                     FNYIQGKKKFVIGSIIADGILLLIPSTMDCLTYITSIIIYKYREVSVYDFNFESTYIF
                     YDDPQICILAFLLIFIPLFLSAVIAIDKQKYTLAILALLPGVFIELLFTI"
     gene            complement(456..1361)
                     /locus_tag="PLK68_11705"
     CDS             complement(456..1361)
                     /locus_tag="PLK68_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRM91111.1"
                     /translation="MFRYRLAYLLLIGFGVFFYIAFVGYFSYYFLLLILILPVLSLFY
                     LVMSFKFTKLDFAILNQKVIQDDFVKIKIIKDNLGLGAIRFVVEDQKYLIKGNQDGLT
                     LVFKHCGGRNFIINTYYQYDCLNLFCIKKRCHYEIPITIYPKRVELDFKEYAHQLPRV
                     GDEVYAVNRKGDDPTEIYDIHKYQEGDQLKNIHWKLSARYQDILVKDNAMLVGEVINL
                     YVSFDDNDDHNDLVFGYLDTFCGFLLKRQIGFLLSNKEIKSIQEYDEMFKYLLWNKEY
                     QSDISKHNYEFVISYNGIMKVEGGR"
     gene            complement(1364..2296)
                     /locus_tag="PLK68_11710"
     CDS             complement(1364..2296)
                     /locus_tag="PLK68_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM91112.1"
                     /translation="MNEKLELLINEVKKAVLGKDDVLKKVVIALLANGHILLEDIPGV
                     GKTNLAVALTKAIQLDYQRVQLTSDVLPSDLLGYSVYDFETKEMTFKKGPLFTNIFLA
                     DEINRTSSKTQSALLQVMEEGMISVDGLTYPTAKPFIVIATQNPFGSAGTQMLPDSQL
                     DRFMMRLSLGYPDEMSEIEIINRKKSGNPINSINPVLSPQELLMMQEAVTKIHLDESL
                     VKYIVQIVQQTRIHEKIELGASPRASIALMKAAQASAYLNGRDYVIVEDINENLYETL
                     NHRIFLMPSVKKNRENFQIIMEDIMMKIAPIAYK"
BASE COUNT          895 a          436 c          263 g          815 t
ORIGIN      
        1 aatcgtaaat aataattcaa taaaaacacc tggtaataaa gccaaaatag ctaatgtata
       61 tttttgttta tcaattgcaa ttacagctga taaaaataat gggataaaaa tcagcaaaaa
      121 agctaagata caaatttggg gatcatcata aaaaatatac gtactttcaa aattaaaatc
      181 atatacagaa acttcacgat acttataaat aataattgaa gttatgtatg ttaaacaatc
      241 catcgtacta ggaatcaata ataagattcc atcagcaata atactcccga taacaaactt
      301 ctttttacct tgaatataat taaatcccaa atatattaac aaacctacta taatacaaga
      361 aataaaaata aaatgattgt tcgaactaaa attaaaagaa taaactaacg agcctactga
      421 accaataatt cctaataaat aaattaataa cttcatcagc ggccaccttc cactttcatg
      481 attccattat agctaataac aaactcataa ttatgtttag aaatatctga ctgatactct
      541 ttattccata acaaatattt aaacatttcg tcatattctt gaatactttt aatttcctta
      601 tttgataata gaaagccaat ttgtcgtttt aataaaaaac cacaaaaggt atcaagataa
      661 ccaaaaacta aatcattatg atcatcattg tcatcaaaac taacatacag attaattacc
      721 tcacctacta acatcgcatt atctttaact aaaatatctt gataacgggc actaagtttc
      781 cagtgaatat ttttcaactg atccccctct tgatatttgt gaatatcata tatttctgta
      841 ggatcatctc ctttacgatt cacagcataa acttcgtcac caactcgtgg tagttgatgt
      901 gcgtactctt tgaaatctaa ttctaccctc tttggataaa tagtaattgg tatctcataa
      961 tggcatctct tttttatgca aaatagattt agacaatcgt attggtagta tgtattaata
     1021 ataaaatttc ttcccccaca atgtttaaat actaaagtta atccgtcttg atttcccttg
     1081 atcaaatact tttgatcctc aacaacaaac cttattgctc caagccctaa attatcctta
     1141 attattttaa tctttacaaa gtcatcttga ataacttttt gattcaaaat agcaaaatcc
     1201 agcttagtaa atttaaaact cataactaaa taaaaaagtg ataaaactgg taaaatcaga
     1261 atcaataaaa gaaaataata tgaaaaatat ccaacaaaag caatatagaa aaatacccca
     1321 aatccaatta ataacaaata agcaagacga tagcgaaaca taactactta tatgcaattg
     1381 gtgcaatctt catcatgata tcttccataa ttatttgaaa attttcacga ttctttttaa
     1441 cacttggcat taagaaaatc cgatgattta gggtttcata taagttttcg ttaatatctt
     1501 caacaataac atagtcccga ccatttaaat atgcactagc ttgagccgct ttcattaatg
     1561 caatcgaagc acgaggagaa gcccctaatt caattttttc atgaattctt gtctgttgaa
     1621 cgatttgaac aatatatttc actaaacttt catctaaatg aattttagta actgcttctt
     1681 gcatcatgag caattcctga ggcgataaaa ccgggttgat tgaattaatt ggatttccac
     1741 ttttttttcg atttataatt tcaatctcac tcatttcatc aggataacct aatgataaac
     1801 gcatcataaa acgatccaac tgagaatctg gtaacatttg tgttccagca cttccaaacg
     1861 gattttgtgt tgcaataaca ataaacggct tagcagttgg ataagttaac ccatcaacac
     1921 taatcattcc ttcttccata acctgcaata gtgctgattg tgttttactt gacgtccgat
     1981 taatttcatc agccaaaaag atatttgtaa atagcggtcc ttttttaaaa gtcatttctt
     2041 ttgtttcaaa atcataaaca gaatacccta ataaatcact tggtaaaaca tcagaggtta
     2101 attgaacccg ctgataatct aactgtattg cctttgttaa agcaaccgct aaattagttt
     2161 tcccgacccc aggaatatct tcaagtagaa tatgtccatt agctaataga gcaataacta
     2221 ctttttttaa aacatcatct tttcctaata cagccttttt tacttcatta atcaatagtt
     2281 ctaatttttc attcatagtc cctctcctta tatttctact acaatatcta aactaaaccc
     2341 taagttaatg ccattactat aagactattt tatcatatta ttagattatt taattattaa
     2401 tttgtctta
//
LOCUS       DAOMTA010000477         2408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2036_length_2408_cov_2.552061, whole genome shotgun sequence.
ACCESSION   DAOMTA010000477 DAOMTA010000000
VERSION     DAOMTA010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2036_length_2408_cov_2.552061"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..891)
                     /locus_tag="PLK68_11715"
     CDS             complement(139..891)
                     /locus_tag="PLK68_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91113.1"
                     /translation="MNLLISRILTYLNGTLLYDSHYRFCKFIVYHYLELEDLSFNEVS
                     EKSQISKEDILRFCALLGFDDYDTFKAELLRSHMIRLDQIRARMLGVNSEQLIDEMEK
                     SCSNEVMSEYISTICEAIFKAKRVVLIGALYPMSIAVEFQTDLVSFGKPVIQYHNFDK
                     DIQLDENDVTIFVSATGRSMNSFVEIKKELRVDLTTSILITQNKTYALDEYKISDYVI
                     QVPGKFDGINFNYQIMTICDLLRVHYYQQYYL"
     gene            complement(948..1694)
                     /locus_tag="PLK68_11720"
     CDS             complement(948..1694)
                     /locus_tag="PLK68_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM91114.1"
                     /translation="MPKNIYVLSDLHGHYNIFIKMLEKINFSDDDVLYILGDCCDRGP
                     DSLKIYLYIQKFDNIHLIKGNHEIMMRDAFKVDDPASSQGRMWAQNGGNKTFHSYHEY
                     LHKKAFNDCDYKVIKAAFYKMMIDYIDRCPSFIELNCNGQDYVLIHAGINPEKGLYEQ
                     TEDECAWMREYFFMSKGLDNKIIIFGHTPTCYIHQASGCFDVWYDPVFKDKIGIDGGL
                     GPFDKGQLNCLCLNTQEVFVIKKSELAIQE"
     gene            complement(1836..>2408)
                     /locus_tag="PLK68_11725"
     CDS             complement(1836..>2408)
                     /locus_tag="PLK68_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91115.1"
                     /translation="IYSYFPVILKINQLLQTTDRLNIAIDGKCGAGKSTLGEMIKAIF
                     ETNLFKMDDFFLQPFQRTAARLNEPGGNIDYERFKETVIVPLQHQESVKYQRFDCSKM
                     ALEAHVELIPYSRFNVIEGTYSMHPYFGMFYDFTIALNVSDDIQAKRILMRNGEMMYE
                     KFKKIWIPLENKYFDQYNIFNVADLQYYSN"
BASE COUNT          888 a          430 c          263 g          827 t
ORIGIN      
        1 aatggattta aaaataaaaa ttaattatta tataaaaggc tgtaaaacag tcttttattt
       61 tttatataga ttaaaaggat ctccattttg gaaatcctct tttactaatt aacaaaaaaa
      121 gataaattat tattcatttt acaaatagta ttgttgataa taatgtactc ttaataaatc
      181 acaaatagtc ataatttgat aattaaaatt gattccatca aattttcccg ggacttgaat
      241 tacataatca gaaatcttat attcatctaa agcatatgtt ttattttgag taattaaaat
      301 agatgttgtt aaatcaaccc gtaattcttt ttttatttca acaaatgaat tcattgacct
      361 acctgttgca gacacaaaaa tagtaacatc attttcatca agctggatat ctttatcaaa
      421 attatgatac tgaattactg gttttccaaa actaactaag tcagtttgaa attctactgc
      481 aatagacatc ggatataaag ctcctattaa aacaacacgc ttagctttaa agatagcttc
      541 acaaatagtc gagatatatt cactcattac ttcattcgaa caagattttt ccatttcatc
      601 aatcagctgc tcactattaa ccccaagcat tcgagcccga atctgatcta aacgaatcat
      661 atgtgatctc aacagttcgg ctttaaatgt atcataatca tcaaaaccaa gcaaagcaca
      721 aaaccttaaa atatcctctt tactgatttg acttttttca ctaacttcat tgaaagataa
      781 atcctctaat tctaagtaat gataaacaat gaatttacaa aaacgataat gtgaatcata
      841 aagcaatgtc ccatttaaat acgttaatat tctacttatt aataaattca taaaatacct
      901 cccttgattt aattctactc ttattaattt taaaattaga gttttttcta ttcctgaata
      961 gccaattcac tttttttaat tacaaatact tcttgagtat ttaaacataa acaatttagc
     1021 tgccctttat caaatggtcc taaaccgcca tcaatcccga tcttatcttt aaaaaccgga
     1081 tcataccaaa catcaaaaca gccacttgcc tgatgaatat aacaagttgg cgtatgacca
     1141 aaaataataa tcttattatc caaacctttt gacataaaga aatactcgcg catccatgca
     1201 cattcatctt cagtttgttc atataacccc ttttcaggat tgattcctgc atgaatcaat
     1261 acataatctt gaccattaca attcagttca ataaatgaag gacaacggtc aatgtaatca
     1321 atcatcatct tataaaacgc cgctttaatt actttataat cacagtcatt aaatgctttt
     1381 ttatgtaaat attcatggta gctgtgaaat gttttatttc caccattttg agcccacatc
     1441 cggccttgtg atgaagcagg gtcatcaact ttaaaagcat cacgcatcat aatctcgtga
     1501 tttcccttaa ttaaatgaat gttatcaaat ttttgaatat ataaataaat ttttaagcta
     1561 tctggacctc gatcacaaca atcccctaaa atatatagaa catcatcatc agaaaaatta
     1621 attttttcca acattttgat aaaaatattg tagtggccat gaaggtcaga aagtacatat
     1681 atatttttag gcattactat caccctttct tatttaagtt tatcattaaa caaacatttt
     1741 ttaaacagat atgctaaaag taatcagtaa ttttttgatt taataaggct ttttcacaaa
     1801 ataaaaacag caatttattt ccttaaaatt gctgcttaat tactataata ttgcaaatcc
     1861 gcaacattaa aaatattata ctgatcaaaa tatttattct ctaaaggaat ccagattttc
     1921 ttaaatttct catacatcat ctcaccatta cgcattaaaa tacgcttagc ttgaatatca
     1981 tcagatacgt tcaaagcaat cgtgaaatca taaaacattc caaaatacgg atgcatactg
     2041 taagttccct caataacatt aaagcgagaa tatggaatta attctacatg agcttctaaa
     2101 gccatctttg aacaatcaaa acgctgatat ttaacgcttt cctgatgttg caaaggaaca
     2161 attactgttt ctttaaaacg ttcataatca atatttccac ccggttcatt taaacgagca
     2221 gcagttcgtt gaaaaggctg taagaaaaaa tcatccattt taaaaagatt tgtttcaaaa
     2281 attgccttaa tcatttcacc aagggtcgac ttccctgcac cacattttcc atcaatagca
     2341 atatttaaac gatcggttgt ttgcaataat tgattaattt ttaaaattac gggaaaatag
     2401 ctataaat
//
LOCUS       DAOMTA010000478         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2047_length_2400_cov_2.212367, whole genome shotgun sequence.
ACCESSION   DAOMTA010000478 DAOMTA010000000
VERSION     DAOMTA010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2047_length_2400_cov_2.212367"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /locus_tag="PLK68_11730"
     CDS             complement(<1..2354)
                     /locus_tag="PLK68_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRM91116.1"
                     /translation="MYVQYTGNSNEKIAVRVSGGEKIATLDLYQVSDENERLEKVKTY
                     LQSLQTQINKMASKHEELHRDDNSVNYDYDEKNCILGATEIMLDHMLYSVSGKQIMAG
                     LKGTTLDEKANQLLNSLNAMDQMMELFYQNKGLNENAAAINDRYPAQHLNIRYQRMFA
                     GAFMYAGGNHIGIEWGSVSGLSNGIPFEAAENGKYLSGSLFGWGIAHEIGHNINQGSY
                     AIAEITNNYFSLLSQNRDSNDTTRFKYPDVYEKVTSNTVGMSSNVFTQLAMYWQLHLA
                     YDQNYHYKLYDSHEEQLNSLFFARVDYYARNPGKVNIPEGGTALKLNSDAQQNFVRLA
                     SAAANKDLTDFFTAWGIIPNEETKAFISQFEAETDKIQYLDDDSMAYRLDGKARMSVD
                     TEINASLSNDKNSNQVTLTISNTNKVDGAMLGYEILRNGQAVGFVNAETGETTFTDTV
                     STVNNRVFTYSIIGYDKLLNRTAELTLKPIKISHDGSLDKDQWLIETNLISDNDEAVG
                     DEDSTPCLPEQKAVNDLIDNNYQNVYKGSTDTKQGEFIVSLNTLADITGVKLTNPSFN
                     QVQIYVSDDKDNWTLVKEGSLKTGEENKLYFSQIVNDELENNKSLVSYSSSYIKIVAV
                     DENDISLSEIDVLGPTGDNIDMSQENSIGILSGDYKLDANNTIPKGSLIFTGTYAGNP
                     AYNVVELRYNKDEILSGYQAIFAEDPGDGDLADVSEGTWIYYLIPETDENGKIIENSF
                     GYQDDEGNFVKVELPGSIKPELYRVNDAQTNAGQRLVSDSFEVK"
BASE COUNT          757 a          445 c          298 g          900 t
ORIGIN      
        1 attttcactt caaaagaatc acttactaaa cgttgaccag catttgtttg agcatcgtta
       61 acgcgataca actctggttt aattgagcca ggaagttcaa ctttaacaaa atttccttca
      121 tcatcttgat atccaaagct attttctatg atttttccat tttcatcagt ttcaggaatt
      181 aaataataaa tccaagttcc ttcactaaca tcagctagat ccccatctcc aggatcttct
      241 gcaaaaatag cttgatagcc acttaaaatt tcatctttat tatatcttaa ttccacaaca
      301 ttataagctg gatttccagc atatgttcct gtaaagatca atgatccctt tggaattgta
      361 ttatttgcat ctagtttata atcaccgctt aatataccaa tactattttc ttgcgacata
      421 tcaatattat cacctgttgg tcctaagaca tcaatttccg ataaactaat atcattttca
      481 tcaacagcaa caatcttaat ataactactt gaatatgaaa ccaatgattt attattttct
      541 aattcatcat taacaatctg actaaaatat aatttatttt cttcacctgt ttttaatgag
      601 ccttctttta ctaaagtcca gttgtcctta tcatcactta catagatctg tacttgatta
      661 aatgacgggt tagttaattt aaccccggtt atgtcagcca aagtatttag tgaaacaata
      721 aattcacctt gttttgtatc tgtactccct ttataaacat tttgataatt gttatcaatt
      781 aaatcgttta ctgctttttg ttctggtaaa catggtgttg aatcttcatc accaactgct
      841 tcatcattat ctgaaataag attagtttca atcagccatt gatctttatc aagacttcca
      901 tcatgagaaa ttttgattgg ttttaaagtc aactctgcag ttcgatttaa taatttatca
      961 tatccaatga ttgaataagt aaaaacacga ttattaacag ttgaaacagt atctgtaaat
     1021 gttgtttcac cagtttctgc atttacaaat ccaactgctt gaccgtttct taaaatttca
     1081 taacctaaca ttgctccatc gactttattt gtgttagaaa tagttaatgt tacttgatta
     1141 ctatttttgt catttgataa tgatgcatta atttcagtgt ctacagacat tctagcttta
     1201 ccatctaaac gataagccat tgaatcgtca tctaaatatt gtattttatc tgtttctgct
     1261 tcaaattggc taataaatgc tttagtttct tcatttggaa taattcccca tgctgtaaag
     1321 aaatctgtta aatctttgtt agctgctgct gaagctaaac gtacaaagtt ttgttgagca
     1381 tcactgttta attttaatgc agtaccacct tcaggaatat tcaccttccc aggattacga
     1441 gcataataat ctactcgagc aaagaacaat gaatttaatt gttcctcgtg tgaatcatat
     1501 aatttataat gataattttg atcataagct aaatgtaatt gccaatacat tgccaattgt
     1561 gtaaagacat tacttgacat tccaaccgta tttgaagtta ctttttcata aacatctgga
     1621 tatttaaatc ttgttgtatc attagaatca cgattttgtg ataataatga gaaatagtta
     1681 ttagttattt ctgcaattgc ataacttcct tgattaatgt tatgtccaat ttcatgggca
     1741 attccccaac caaataaaga acccgataaa tattttccat tttctgctgc ttcaaatgga
     1801 attccatttg ataaaccaga tactgaaccc cattcaattc cgatatgatt tccaccagcg
     1861 tacataaaag caccagcaaa cattctttga taacggatat ttaaatgctg agcaggatag
     1921 cgatcattaa tagcagctgc attttcatta agccccttat tttgatagaa taattccatc
     1981 atttgatcca ttgcatttaa tgaatttaat aattgatttg ccttttcatc taatgttgta
     2041 ccttttaaac cagccatgat ttgtttacct gatactgagt acagcatatg atcaagcata
     2101 atctcagtag ctcctaaaat acaatttttc tcgtcataat catagttaac actattgtca
     2161 tcacgatgaa gttcttcgtg ttttgaagcc attttattaa tttgtgtttg taaactttga
     2221 agataagttt ttactttttc taatcgttca ttttcatcac taacttgata taaatctaat
     2281 gtagcaatct tttcaccgcc tgatactcta acagcaatct tttcattaga attaccagta
     2341 tattgaacat acacagatcc acctttttca actccgatag actgtaattc tttcatactg
//
LOCUS       DAOMTA010000479         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2048_length_2399_cov_2.537543, whole genome shotgun sequence.
ACCESSION   DAOMTA010000479 DAOMTA010000000
VERSION     DAOMTA010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_2399_cov_2.537543"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..753
                     /locus_tag="PLK68_11735"
     CDS             205..753
                     /locus_tag="PLK68_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM91117.1"
                     /translation="MKKLLSSLMVACLMFVATISTVGAVGSITADEQKIIDALEAGVT
                     VDGVKVNLKAADINAAKNYLTNNDVTAAQVTTILNNIEGAKSYMQTNHIKDIASIKGA
                     HATAILGFAQAAADVLGLTIKVGADGTVTVYKGSEVVYSTTNVIKKTGYDFTQTAVMA
                     AGLVAVLFGAGYYAKRKQLLVK"
     gene            786..1424
                     /locus_tag="PLK68_11740"
     CDS             786..1424
                     /locus_tag="PLK68_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HRM91118.1"
                     /translation="MALVLVPLSFTIICYLVLFLIISPIIEPVMSIYSLVASEHAPNL
                     NDNSNKDLYKNKLVSSATIKASEVVMPSYGDLFGKITIDSVNKTINLYYGDGNDQLRK
                     GAGLFNGSFLPGFNRTSLIAGHSLPYFECLGDVNVGDIIKISTHYGDFEYKITDTKIG
                     KATDESNYDLAQSEKEQLILYTCYPFELIGYKADRLFVYADKISGPTILEGQ"
     gene            1426..>2157
                     /locus_tag="PLK68_11745"
     CDS             1426..>2157
                     /locus_tag="PLK68_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91119.1"
                     /translation="MARKKSSSRTTLSYILSLLIAIFLSILVVLTVTRFTVMSPSFLI
                     SKMDGADFYKQSVVSLNEEIQQETQSTGFPIEMFENYVDEDTAKTAMEKYINNAFDGG
                     ETTINTTEFETKLTQDIDNYLTTNNIIINSESQEAISVLKSNLIDLYKSYLSFPYLAL
                     VIDVINTYDGIFLIAAAALVVLIGLASFLLYRLYSHYQGRRRYFSYALSASGLMCFAL
                     PFFIYIGKFIDKISLSPAYFYNFFTS"
     assembly_gap    2158..2167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          816 a          345 c          376 g          852 t
ORIGIN      
        1 attattatat cataaataaa ttgtttttta tatatcaaat ttttaatatt tttttgtatt
       61 ttttatcatt gtcacaaaat tatctcaaat ttaaataact aaatttaact aattacttgt
      121 tattatgcaa aaaaagatat attatatatg tgtatatttg tgtatctttc gcaaatattc
      181 taaaactaaa aaggagggga caagatgaag aaacttttaa gttctttaat ggtagcttgc
      241 ttaatgtttg ttgcaactat ctcaactgtt ggtgctgttg gttctatcac tgctgatgaa
      301 caaaaaatca tcgatgcttt agaagctggt gttacagttg atggagttaa agttaatcta
      361 aaagctgctg atattaatgc tgctaaaaat tacttaacta acaatgatgt tacagctgct
      421 caagtgacta ctattttgaa caacattgaa ggagcaaaat cttatatgca aactaatcat
      481 attaaagata tcgcttctat caaaggtgct catgccactg caattttagg tttcgctcaa
      541 gctgcagctg atgtacttgg gttaactatt aaagttggtg ctgatggtac tgtcactgtt
      601 tacaaaggta gcgaagttgt ttattcaact acaaacgtta tcaaaaagac tggttatgat
      661 ttcactcaaa ctgctgtaat ggcagctgga cttgtagctg tattattcgg tgcaggatat
      721 tacgctaaga gaaaacaact tttagttaaa taatcgtgcg attaaaacat aagcaaaaaa
      781 agataatggc actagtatta gtgccattat cttttaccat tatctgctac ctagtattat
      841 ttttaataat tagtccaatt attgaaccag tcatgtctat ttacagtctt gttgctagtg
      901 aacatgcacc aaatttaaat gataacagca acaaagatct atataaaaat aaactagtta
      961 gttctgctac tattaaagct tcagaagtag taatgccttc ttatggtgac ctatttggta
     1021 aaataaccat tgattctgtc aacaagacaa ttaacctata ctatggtgat ggtaatgatc
     1081 agcttcgtaa aggcgctgga ttattcaacg gttcattttt accaggattt aaccgtactt
     1141 cattaattgc cggtcactca ttgccttact ttgaatgttt aggtgatgtc aatgttggag
     1201 atattattaa aatatctacc cattatggtg attttgaata taaaataaca gatactaaaa
     1261 ttggtaaagc tactgatgaa tcaaattacg atcttgccca aagcgagaaa gaacaattga
     1321 tcttatatac atgttatcca ttcgaattga ttggttacaa agctgaccgt ttatttgttt
     1381 atgcagataa aataagtggt ccaacgatct tggagggaca ataacatggc aagaaaaaaa
     1441 tcaagtagtc gaactacatt atcgtatatt ttatcattat taattgcaat ctttctaagt
     1501 atcttagtag ttctaacagt aactcgtttt actgttatgt ctccaagttt cttaatttct
     1561 aaaatggatg gagctgattt ttataaacaa tcagttgtat cattaaatga agagatccag
     1621 caagaaacac agtcaacagg atttcctata gaaatgtttg aaaactatgt tgatgaagat
     1681 actgctaaaa cagcaatgga aaaatacatt aataatgcct ttgatggtgg tgaaaccact
     1741 attaacacaa ctgaatttga aacaaaactt acccaggata tagataacta tttaacaact
     1801 aataatatta ttattaatag tgaatcacaa gaagcaatta gcgtactgaa aagtaacttg
     1861 attgatttat acaaaagcta tttatcattc ccttatctag cccttgttat tgatgttata
     1921 aatacatatg atggaatctt tttaattgcg gcagcagctt tagttgtttt aatcggatta
     1981 gcaagtttct tattatatcg cctatatagt cattatcaag gtagaagacg ctatttctca
     2041 tatgccctat cggcttctgg tttaatgtgt ttcgctttac cattctttat ctatattggt
     2101 aaatttattg ataaaatcag tttatcacct gcatatttct ataatttctt tacaagtnnn
     2161 nnnnnnnaaa ctcttacatg ttgacatttg taatcgttgg tttagtaatg attgttttag
     2221 caaatattgt agcgtatata aaatttgaac gaaaataatg cgcttgtaat taatacaagc
     2281 gttttatttt gcttataaag aaaaaaggct ctaacttaaa ttataggcct tttaactgtt
     2341 atgtgtaatt atcaaatatt tgttcagcgg tcttaattaa agcttttgaa aaaatgcat
//
LOCUS       DAOMTA010000480         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2049_length_2399_cov_2.263652, whole genome shotgun sequence.
ACCESSION   DAOMTA010000480 DAOMTA010000000
VERSION     DAOMTA010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_2399_cov_2.263652"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLK68_11750"
     CDS             <1..428
                     /locus_tag="PLK68_11750"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRM91120.1"
                     /translation="AEAVTKASELALEKGARRAIPLNTSGPFHTELLAPASNELKEKF
                     VSVNFNEMQVPVIFNSSAKELESGVSIAQMLEKQVMSSVYFEDSIRYMLENGVDTIIE
                     IGPGKVLSGFIRKIDKTIKTYQVEDQASLEKTLAGLKGE"
     gene            430..1182
                     /gene="fabG"
                     /locus_tag="PLK68_11755"
     CDS             430..1182
                     /gene="fabG"
                     /locus_tag="PLK68_11755"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRM91121.1"
                     /translation="MKLKDKVVLVTGGAQGIGKEICLTCAREGAKIIVNYVNFGNNKK
                     IAEATKSELEAIGATVMLAQANVASFEETETMFKEIIKEFGRIDVLVNNAGITKDGLL
                     MRMKENDFDAVIDVNLKGTWNCMKHATKIMMKQRYGRIISMSSVVGVMGNAGQVNYSA
                     SKAGIIGMTMSLAREVGSRGITVNAVAPGFIQTAMTDVLPEDIKESMAKQIPLGTFGQ
                     VSDIANTVVFLASDDARYITGQTIHVDGGMAM"
     gene            1194..>1399
                     /locus_tag="PLK68_11760"
     CDS             1194..>1399
                     /locus_tag="PLK68_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRM91122.1"
                     /translation="MERRRVVVTGLGAINAIGHNVEETWKSIKEGVCGIDEITNYDKE
                     LTKVKIAGEVKNFNAADYLDKKAVR"
     assembly_gap    1400..1409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1410..>2399
                     /gene="fabF"
                     /locus_tag="PLK68_11765"
     CDS             <1410..>2399
                     /gene="fabF"
                     /locus_tag="PLK68_11765"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRM91123.1"
                     /translation="FTQFGVIAAKEAFKDAGLKIEEIDADRFGVVVSSGIGGLATIEK
                     DHTKGLEKGYDRVSPFFVPMSITNLAAGNIAIELGAKGLCTCPVTACAGGTNAIGDAF
                     RNIRDGYQEIMVAGGAEASITPLGIGGFSSMKALCDNNDPKRASIPFDEERCGFVMGE
                     GAGIVVLEELEHAKARGAHIYCEMVGYGVSCDAHHITAPLEDGSGGAKAMINAIKDAG
                     LVAEDVTYINAHGTSTPLNDKGETMAIKSALGEAAKKVAISSTKGNTGHCLGAAGGIE
                     GVICVKAIEEGFIPATINYQKPDPLCDLDIVPNTGRNQDVNVAMSNSLGFGGH"
BASE COUNT          799 a          289 c          572 g          729 t
ORIGIN      
        1 aggctgaggc agttactaag gctagcgaat tagcattaga aaaaggtgct cgtcgggcaa
       61 ttcctttaaa tacaagtgga cctttccata cagaattatt agcaccagct agtaatgaac
      121 ttaaagaaaa gtttgtaagt gttaatttta atgaaatgca agttcctgta atattcaata
      181 gttcggctaa agaacttgag tctggagtta gtattgctca aatgttagaa aaacaagtaa
      241 tgtcttcagt gtattttgaa gatagtattc gttatatgtt agaaaacggg gttgatacaa
      301 ttattgaaat cggtcctggt aaagttttaa gtggatttat tcgtaagatt gataagacaa
      361 ttaaaactta tcaagttgaa gatcaagcat cattagaaaa aacattagcg ggattaaaag
      421 gagaataaga tgaaattaaa agataaagta gttttagtaa ctggtggagc ccaaggaatt
      481 ggtaaagaaa tttgtttaac ttgtgctcgt gaaggtgcta agatcattgt aaattatgtt
      541 aattttggta ataataaaaa aattgctgaa gcaactaaat cagaattaga agcaatcggt
      601 gcgactgtta tgttagctca agctaatgtt gcttcatttg aagaaacaga aacaatgttt
      661 aaagaaatca ttaaagaatt tggtcgaatc gatgttcttg taaataatgc tggaattact
      721 aaagatggat tgttgatgcg aatgaaagag aatgattttg atgcagttat tgatgttaat
      781 ttaaaaggaa catggaattg tatgaaacat gcgactaaaa ttatgatgaa acaacgttat
      841 ggtcgtatta tttcaatgtc atcagtagtt ggggtaatgg gaaatgctgg tcaagtaaat
      901 tattcagcaa gtaaagctgg aattattgga atgacaatgt cattagcgcg cgaagttggt
      961 agtcggggaa ttactgtgaa tgcggtagca ccaggtttta ttcaaactgc aatgactgat
     1021 gttttacctg aagatattaa agaaagtatg gctaaacaaa ttcctttagg aacttttgga
     1081 caagtaagtg atatcgctaa tacggtagta tttttagcaa gtgatgatgc tagatacatt
     1141 actggtcaaa ctatccatgt tgatggcgga atggcaatgt aaggagagag acaatggaaa
     1201 gaagaagagt tgtagtaaca gggcttggag caattaatgc aattggtcat aatgttgaag
     1261 aaacttggaa aagtattaaa gaaggtgttt gtggaattga cgagatcaca aactatgata
     1321 aagaattaac aaaagtaaaa attgcaggtg aagtaaaaaa ttttaatgct gctgattatt
     1381 tagataaaaa agcagttcgn nnnnnnnnnc tttactcaat ttggtgtaat tgctgctaaa
     1441 gaggctttta aggatgctgg tttaaagatt gaagagattg atgctgaccg ttttggtgta
     1501 gttgtgtcaa gcggaattgg agggcttgca acaattgaga aagatcatac taaggggtta
     1561 gaaaaaggtt atgatcgtgt ttcgccattc tttgtgccaa tgtcaattac aaatttggca
     1621 gcgggaaata ttgcaattga attaggggca aaaggattat gtacttgtcc ggtcacagct
     1681 tgcgctggtg gaactaatgc gattggtgat gcgtttagaa atatccgtga tggttatcaa
     1741 gaaattatgg ttgctggtgg ggcagaagct agtattactc cacttggtat tggaggcttt
     1801 tcatcaatga aagctctttg tgataataat gatccgaaac gggcgtcaat tccttttgac
     1861 gaggaacgtt gtggatttgt tatgggtgag ggtgctggaa ttgttgtact tgaagaatta
     1921 gaacatgcta aagctcgtgg cgctcatatc tattgtgaaa tggtcggata tggtgtcagt
     1981 tgtgatgctc atcatattac tgcaccttta gaagatggct ctggtggggc taaagcgatg
     2041 atcaatgcaa tcaaggatgc tggtttagta gctgaagacg taacttatat taatgcacat
     2101 ggaactagta cccctttaaa tgataaaggt gaaacaatgg caattaaatc tgctttaggt
     2161 gaagctgcta aaaaggttgc tatttcttcg acgaaaggaa atacggggca ttgtttaggt
     2221 gctgccgggg gaatcgaggg ggtaatttgt gttaaagcaa tcgaagaggg atttattcca
     2281 gctacgatta attatcaaaa accagatcca ttatgtgatt tagatatcgt tccaaatact
     2341 ggtagaaatc aagatgttaa tgtagctatg tcaaattcat taggatttgg tggtcataa
//
LOCUS       DAOMTA010000481         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2056_length_2392_cov_2.241763, whole genome shotgun sequence.
ACCESSION   DAOMTA010000481 DAOMTA010000000
VERSION     DAOMTA010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2056_length_2392_cov_2.241763"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLK68_11770"
     CDS             <1..338
                     /locus_tag="PLK68_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM91124.1"
                     /translation="KIQYLDLVKNDFIQNEIIENIEEYRELLIRESKEEIKDKNIYVD
                     FEKILIVVFTFFTTISICGRGRISGEIIIIIYLLILICSTALLHYPQSRKFFYQLYQK
                     LQEYKRKDK"
     gene            335..1903
                     /locus_tag="PLK68_11775"
     CDS             335..1903
                     /locus_tag="PLK68_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91125.1"
                     /translation="MIWLIIISIVLIAGVLGFLKILVTKKYLIHQYNLGVGVQYRDFT
                     KEYYHYNKERSIDEETFIDFEMPQILKKINFTYSEIGNEYLYNLFFRENNNLDTQESI
                     IDKLNCQKKLAESIYQLNKLNKAYVPILSIKRSLLNISAKSCLLAGLFLILDVVSIIA
                     AIIDIENVYFVVIMLLLSILMNTQLSQKTGKISLQISLINDMLQITNKLLKINIYPDS
                     NQTLVMNSFIHLKKMTKTTYYFNKIKQFDIFNILELMKSLFFIDIFQAVKLSRKVDQV
                     QNDIMVLYENIGLLDVCIMIKVIRSLYDTCIPTILKDERVEIVEGYHLLIEKPIKNTV
                     VISNDTIVTGSNASGKSTFLKMLGANLLLAKTLNISFAKEFKYYPFALISSIHMKDDI
                     MNGDSFYVKEIKRLKQITEFAKKQKSLVLIDEILKGTNEKERIIIALALMKYLFKCNS
                     MTIITTHDIELTETFDQVDKYCFNDIKKDNKIIFDYLIKKGVCTVGNAIAIVKTLDFD
                     QEILKEINDKIEVF"
     gene            complement(1970..>2392)
                     /locus_tag="PLK68_11780"
     CDS             complement(1970..>2392)
                     /locus_tag="PLK68_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM91126.1"
                     /translation="LTTDAPTLKHLHLVDEGIENLLKEKVVITNHMDELITSLTSKRY
                     TRSRIQRMLIHILMNNSKEDIAEAMQIDYIRVLKMNNVGQAYLNKIKKSCEYKLVTNF
                     SSYHHPALELEFRATKLLSCLSKNPNQLISLEYKSIPK"
BASE COUNT          886 a          246 c          379 g          881 t
ORIGIN      
        1 aaaagattca atatcttgat ctagttaaaa atgattttat tcaaaatgaa attattgaga
       61 atatagaaga atatcgagaa ttattaattc gtgaatcaaa agaagaaatc aaagataaaa
      121 atatatatgt tgattttgaa aaaatactaa ttgttgtctt tacatttttt acaactataa
      181 gcatatgtgg aagaggtagg atatctgggg aaataataat tattatttat ctacttattt
      241 taatttgtag tacggcttta ttgcattatc cgcaatcgcg taaatttttt tatcaattgt
      301 atcaaaagtt gcaagaatat aagaggaaag ataaatgatt tggttaatta ttataagtat
      361 cgtgttgatt gcaggagtat taggtttctt aaaaatatta gttacgaaga aatatttgat
      421 ccatcaatat aatttaggag ttggtgtgca gtatcgggat tttactaaag agtattatca
      481 ttataataaa gaacgaagta ttgacgaaga aacttttata gattttgaaa tgccgcaaat
      541 tttaaagaaa attaatttta cttatagtga gataggaaat gagtacttgt ataatttatt
      601 ttttagagaa aataataatt tagatactca agaaagtatt attgataaat tgaattgtca
      661 aaaaaaatta gcagagagta tttatcagct gaataaactt aataaagcat atgtaccgat
      721 cttatctatt aaaagaagct tgctaaatat ttcagctaaa tcgtgtttat tagcagggct
      781 gtttttgata cttgatgtgg ttagtataat agcagcaata attgatatag agaatgttta
      841 ttttgttgtt ataatgttat tattgagcat tttaatgaat acccaattat ctcaaaaaac
      901 aggaaaaatt agtcttcaga tatcattaat taatgatatg cttcaaatta ccaataaatt
      961 attaaaaata aatatttatc ctgattcaaa tcagacttta gtaatgaact catttatcca
     1021 tttgaagaaa atgacaaaaa caacatatta ttttaataaa ataaaacagt ttgatatttt
     1081 caatattttg gagttaatga aatcattatt ctttattgat atatttcaag cagttaaact
     1141 tagtaggaaa gtagatcaag tacaaaatga tatcatggtt ctttatgaga atatcggttt
     1201 gttggatgta tgtattatga ttaaggttat tcgtagtctt tatgatactt gtataccaac
     1261 catattaaaa gatgaaagag ttgagatagt agaggggtat catttattga tagaaaaacc
     1321 gattaaaaat actgttgtaa tatcaaatga tacgattgtt actggtagca atgcctctgg
     1381 gaaatctacg tttttgaaaa tgttaggtgc caatttgttg ttagcaaaaa ccttaaatat
     1441 tagttttgca aaggaattta aatattatcc gtttgctttg atctcatcaa ttcatatgaa
     1501 ggatgatatt atgaatggag atagttttta tgttaaagaa atcaaacgtt taaagcaaat
     1561 tactgaattt gctaaaaagc aaaaatcatt agtcttaatt gatgagattt taaaggggac
     1621 aaatgaaaaa gagcgaatca ttattgcgct ggcattaatg aaatatttat ttaaatgcaa
     1681 ttctatgacg attattacta cccatgatat tgaattaaca gaaacctttg atcaagtaga
     1741 taaatattgt tttaatgata ttaaaaaaga taataaaatc atttttgatt accttattaa
     1801 gaagggagtt tgtactgttg gaaatgcaat cgcgatagtt aagacattag attttgatca
     1861 agaaatttta aaggaaatta atgataaaat agaagttttt tgaagtgaac ctcgttgctt
     1921 ggacacacat cacattctaa ataatagagg ttcatttcat tttaggcttc tattttggga
     1981 tacttttata ttctaatgat ataagctgat ttggattttt tgataaacag gataataatt
     2041 ttgttgctct aaattctaat tcaagtgcgg gatgatgata actggaaaaa ttagtgacta
     2101 atttatattc acagcttttt tttattttgt ttaaataagc ctgcccaaca ttattcatct
     2161 ttaatactcg aatatagtcg atttgcattg cttcagcaat atcttcttta ctattattca
     2221 tcaagatatg aattaacatg cgctgaattc gtgagcgtgt ataacgtttc gaagtaaggc
     2281 ttgttattaa ttcatccata tgattagtga taactacttt ttctttaagt aaattttcaa
     2341 ttccttcgtc gactaaatgc aaatgtttta atgttggcgc atcagtagtt aa
//
LOCUS       DAOMTA010000482         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2058_length_2390_cov_2.100642, whole genome shotgun sequence.
ACCESSION   DAOMTA010000482 DAOMTA010000000
VERSION     DAOMTA010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2058_length_2390_cov_2.100642"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLK68_11785"
     CDS             <1..628
                     /locus_tag="PLK68_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM91127.1"
                     /translation="SEHPEIEIVQIQFNYADFNNPSVESKKVYEVCRKFNKPILVMEP
                     IKGGGLANLPVAANRILTDLNKNASNASYALRFAASFEGVYKVLSGVSNLEQMKDNLK
                     VMKNFIPFNDEEYEAVAKVRKILDSLGGIPCTACRYCTDGCPMKISIPDLFGCYNDKK
                     IFNDWNSDYYYGVHTKTNGKASTCIGCQQCPQHLPIIKYLKEVAETFE"
     gene            complement(725..1912)
                     /gene="hydF"
                     /locus_tag="PLK68_11790"
     CDS             complement(725..1912)
                     /gene="hydF"
                     /locus_tag="PLK68_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRM91128.1"
                     /translation="MAGLNKTPGANRLHIGFFGKRNSGKSALINTFVNQEVSIVSSEP
                     GTTTDPVYKAMEIDGLGPCLLVDTAGFDDIGTLGQLRNDKTKKASEKIDIAIILFNDR
                     KICQELDWYHYFKEKHIPTILVISKADLQRNDNLLKKITEATSETPLLISSLTKQGIK
                     ELQEKLLEKVPADFEKESITGELAKKGDLILLVMPQDIQAPKGRLILPQVQVMRELLD
                     KGCQIMAVTTEQLKSALQKLNQAPDLVITDSQVFKYVYENISSESKLTSFSVLMAGYK
                     GDLNYYAKSVEILERLNGDSTVLIAECCSHAPLSEDIGRVKIPRMLKKKFPGIGVEIV
                     SGTDFPDDLTKYDLIITCGGCMFNRRYIMTRVNQAKGQMIPMTNYGIFIAYVNGILDK
                     VIY"
     gene            complement(1915..>2390)
                     /locus_tag="PLK68_11795"
     CDS             complement(1915..>2390)
                     /locus_tag="PLK68_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816059.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRM91129.1"
                     /translation="ISTRESKRVRERVLELGISQISGGSKTSVGGYDEPETEEDTTSA
                     QFDISDNRTLDEVVKWLCELNYIPSFCTACYREGRTGDRFMQLCKSGQISNCCHPNAL
                     MTLKEYSQDYAGDDTKLKADNLIMKELDNITSDKVRKICQERLTMIEDGKRDFRF"
BASE COUNT          777 a          447 c          341 g          825 t
ORIGIN      
        1 aagtgaacat cccgaaatag aaattgtcca aattcaattt aattatgctg attttaataa
       61 tcctagtgtt gaaagtaaga aggtttatga agtatgtcgc aaatttaata aacctatttt
      121 agtaatggaa ccaattaaag gcggtggtct tgcgaatctt cccgtagcag caaatagaat
      181 tttaactgat ctaaataaaa atgccagcaa cgctagttat gctctacgct ttgcagcgtc
      241 ttttgaaggt gtatataaag ttttatcagg tgttagtaat ttagagcaaa tgaaagataa
      301 tttgaaggtt atgaaaaatt tcattccttt taacgatgag gaatacgagg cggtagcaaa
      361 agttcgaaaa atattagatt ccttaggagg aattccttgt accgcttgtc gttattgtac
      421 agacggctgc ccgatgaaaa tttcaattcc tgacttattt ggctgttata atgataaaaa
      481 aatatttaat gactggaata gtgattatta ttatggagtt cacacaaaaa ctaatggcaa
      541 ggcatctact tgtattggat gtcaacaatg tccgcagcat ctgccaatta ttaaatattt
      601 aaaagaagtc gctgaaacat ttgagtaatt aaaaagatac tccatgatgc aaatgtaatg
      661 aaaaggtaga tattagttcc aatatctacc tttattttac ttctgtatca tttgaatagt
      721 ccttttaata tatgacttta tccaagatcc catttacata tgcaataaaa atgccataat
      781 ttgtcatcgg aatcatttgc ccttttgcct gattaactct tgtcataata taccggcgat
      841 taaacataca gccaccacaa gtaataatta aatcgtactt ggttaaatca tctggaaaat
      901 ctgttccact aacaatttca acaccaattc caggaaattt tttctttaac attcgaggaa
      961 ttttaacccg tccaatatct tcacttaatg gtgcatgact acaacattct gcaattaaaa
     1021 ctgtcgaatc accattcagc cgttctaaga tttcaacact tttagcataa taatttaaat
     1081 ctcccttata acctgccatt aaaacagaaa atgatgtcaa tttgctttct gaagaaatat
     1141 tttcataaac atatttaaat acttgtgaat ctgtaataac aagatcaggg gcttgattta
     1201 atttttgtaa agctgatttt agttgttccg ttgttacagc cataatttga caacctttat
     1261 caagcaattc acgcattacc tgaacttggg gtaaaattaa tctgcctttg ggagcttgaa
     1321 tatcttgtgg cattactaat agtataagat cacctttttt tgctaattca cctgtaatgc
     1381 tttctttttc aaaatcagcc ggtacctttt caagcaattt ttcctgtaac tcttttattc
     1441 cttgcttcgt taacgaacta atcagtaatg gtgtttcact agttgcttca gtaattttct
     1501 ttagaagatt atcattcctt tgtaaatcag ctttacttat tactaatatc gtaggaatat
     1561 gcttctcttt aaaataatga taccagtcta attcttgaca aatctttcta tcattaaaaa
     1621 gaatgattgc aatatcaatc ttttcactag ctttttttgt cttatcgttt cgcaattgcc
     1681 ctagagtccc aatatcatca aaacctgcag tatcaactaa caagcatggt cccaaaccat
     1741 caatttccat cgccttatag acaggatcag tagttgttcc cggttcactt gagacaattg
     1801 aaacttcttg attaacaaag gtgttgatca atgccgattt gccactatta cgttttccaa
     1861 aaaaaccaat atggagccga ttagctcctg gtgttttatt taaccctgcc attattagaa
     1921 cctgaaatca cgtttcccgt cttcaatcat cgtaagacgt tcttgacaaa tttttctaac
     1981 tttatcacta gtgatattat ctaattcctt cataattaaa ttatcagctt ttaacttagt
     2041 atcatcaccg gcataatctt gactatattc ctttaatgtc atcaaagcat taggatgaca
     2101 acaattcgag atttgaccag atttacataa ttgcataaaa cgatctcccg ttcttccttc
     2161 gcgataacaa gcagtacaaa aacttggaat ataatttaat tcacataacc acttaacaac
     2221 ctcatctaaa gttctattat cactaatatc aaattgtgca gaagtagtat cttcttcagt
     2281 ttcaggttca tcataaccgc caacacttgt cttagagcca ccactaattt gtgaaatacc
     2341 taactctaac actctttctc gaacccgttt agattcacgt gttgaaataa
//
LOCUS       DAOMTA010000483         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2072_length_2381_cov_1.991831, whole genome shotgun sequence.
ACCESSION   DAOMTA010000483 DAOMTA010000000
VERSION     DAOMTA010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_2381_cov_1.991831"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1039)
                     /locus_tag="PLK68_11800"
     CDS             complement(107..1039)
                     /locus_tag="PLK68_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91130.1"
                     /translation="MVTNSISRQQSNNLNIALVPSNESYGHEFWLKKYSGWKKPILGL
                     IEHGLYLGNNHEKVGDEIEYDLKVIFTYGKYREEILKEAFADIKVIKIGPRIAYARTD
                     EQYKKDITDKLIGNKRTLTIFPAHSITSLKSKYDVKDLMCKAHTICKMYNLENINVCL
                     PYGDIQKGIDKEFEKENCHVVSAGSDSISFLPRLRAIIETSTLTLSNSLGTNLGYCIY
                     MGCQQILIPQEIEYEGKEEAIIEEKKNKGHDYEYYYQRETDSFAELFHINAGIQISKE
                     QYELCDKFWGFTDVKEPKELLNIFEMVHKGKMYK"
     gene            complement(1101..>2381)
                     /locus_tag="PLK68_11805"
     CDS             complement(1101..>2381)
                     /locus_tag="PLK68_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM91131.1"
                     /translation="KMIKKLISEGFLKIFSASTINKIVNFAYSYVLIRVVSKSDYGVY
                     SYAATVYGFLMIFNIIGLDSAALQLYVENEKNEARQKTIFSYCSKVAVFVNCLICIGV
                     LCFSRFIPLSIKGSNEVLGLMFFQPVITAIKVMQQTYLRANVRNNDYAALNIIDSGFV
                     ATLSIIGSITYGISGLIISQYLAGISVILIGRYKYNCSIFAGAKEKKYELDKKSIYQI
                     ALLMAVNNSLATILSLLGTTILGMIISDSEVIASYKVALTLPSALLFIPQAVMIFALP
                     YFIRHKDDYVWTKEKTKLLLGGIGIFNGGVALVSICAAPMIIKILFGSQYLDCVPVFR
                     ILMLSFFFQATFRIPVSNILFSKRKLESNTIASIVGIVLTVGLSFMLVPTLAGVGTAL
                     AYLITYLTVVLILVRHLLVVIKRMKRAYYESKGV"
BASE COUNT          802 a          463 c          289 g          827 t
ORIGIN      
        1 tgaatgtata aatagaacaa gcacaatact catcgcacgt atataatcaa tcacttttga
       61 atctttttca gatagttgat accccatttt aattccttcc atatcattat ttgtacattt
      121 tacctttatg taccatttca aaaatattga gtaattcttt tggctctttt acatcagtaa
      181 agccccagaa cttatcacaa agttcatatt gttcctttga tatctgtatt cctgcattta
      241 tatgaaataa ttctgcaaaa gaatccgttt ctctttgata ataatattcg taatcatgac
      301 ctttgttctt cttttcctca ataattgctt cttcttttcc ctcgtattct atttcctgtg
      361 ggataagaat ctgttgacac cccatataaa tacaataacc taagttagtc ccaaggctat
      421 tggatagcgt taaggttgat gtttcaataa ttgcacgtag tctaggtaaa aaactaatag
      481 aatctgaacc tgcgctaacc acatggcaat tctccttctc aaactcttta tcaattcctt
      541 tttgaatatc gccatacggt aaacagacat taatattctc aagattatac attttacata
      601 ttgtatgagc tttacacatt aaatccttaa cgtcatattt gcttttcaag gaagtaattg
      661 aatgggcagg aaaaattgtc aatgttcttt tatttccaat aagcttatcg gtaatatcct
      721 ttttatattg ttcatctgtg cgcgcatatg ctatacgagg tccaatctta attactttaa
      781 tatcagcaaa agcttctttt aaaatttctt ccctatattt tccataggtg aaaataactt
      841 ttaaatcata ttcgatttca tctccaactt tttcatgatt attccctagg tataatccat
      901 gttcaataag tcccaaaatc ggttttttcc atcctgaata tttcttcaac caaaactcat
      961 gtccatacga ttcgttactc ggaaccaaag caatgttaag attattcgat tgctgtctcg
     1021 aaattgaatt tgtaaccaaa tgaacacacc tatataattt aagctttgat tttgaattaa
     1081 tgttaaattt attaattaca ctaaacacct tttgattcat agtaagccct cttcattctc
     1141 ttaatcacaa ccaacaaatg tctaaccaaa attaaaacaa ctgttaaata tgtaatcaaa
     1201 tatgccaacg ctgttcctac tccggcaagc gttggaacta acataaaact taatccaact
     1261 gttaagacaa ttccaactat tgaagcaata gtattacttt ccaattttct cttagaaaaa
     1321 agaatattag acactggtat tctgaaagtt gcctggaaaa agaatgacaa catcagaatt
     1381 ctaaaaacag gtacacaatc taagtattgt gaaccaaata aaattttgat tatcattgga
     1441 gccgcacata tagataccag cgctacgcct ccattaaata tacctattcc ccccaataaa
     1501 agttttgttt tttcttttgt ccacacatag tcatctttat gtctgataaa ataaggcaaa
     1561 gcaaatatca tgacggcctg aggtataaat aacagagctg atggtaaagt gagtgccacc
     1621 ttatatgaag ctatcacttc actatcacta ataatcattc ccaaaattgt tgttccaagc
     1681 aaactcaata ttgtcgctaa gctgttattg actgccatca acaacgcaat ttgatatata
     1741 gattttttat ctaattcata ttttttctct tttgccccag caaaaatact acaattatat
     1801 ttgtatctac caattaatat aactgaaata ccagcaaggt attggctaat gatcaaccca
     1861 cttattccat atgttataga accaataatt gatagtgttg caacaaaacc agaatcaatt
     1921 atatttagtg ctgcataatc attgtttctt acattagctc gaagatatgt ctgttgcatt
     1981 actttaattg cggttatcac tggctgaaaa aacataagcc ctaaaacttc gttactccct
     2041 ttgattgata acggaataaa tctagaaaaa cacaacacac ctatacaaat aagacaattt
     2101 acaaatactg ctactttgga acaatatgaa aaaattgtct tttgccttgc ttcatttttt
     2161 tcattttcta catacaactg aagagctgcc gagtcgagac ctataatatt aaaaatcatc
     2221 aaaaacccat aaactgttgc agcatatgaa tagactccat aatcgctctt tgaaacaacc
     2281 ctaatcagca cataactata cgcaaaattc acgattttat ttattgttga agcagaaaat
     2341 attttaagaa atccttcgct tattagtttt tttatcatct t
//
LOCUS       DAOMTA010000484         2374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2082_length_2374_cov_2.609314, whole genome shotgun sequence.
ACCESSION   DAOMTA010000484 DAOMTA010000000
VERSION     DAOMTA010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_2374_cov_2.609314"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1477)
                     /gene="melB"
                     /locus_tag="PLK68_11810"
     CDS             complement(128..1477)
                     /gene="melB"
                     /locus_tag="PLK68_11810"
                     /inference="COORDINATES: protein motif:HMM:NF007749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HRM91132.1"
                     /translation="MYVVFYRCSASFVGTLFFLAKFWDAVNDLGMGMIVDNTHSRWGK
                     FRPWLAIGTIVNAIILVALFTDWGLSGTSLYIFAAIMYIVWGMTYTVMDIPYWSMLPN
                     LTSNPEERDRVAVIPRIFASIGGSLLVGGFGLQIMDFLGNGDAQVGYTNFAIVIAIIF
                     IVTVGITVVNVKSADRVEAKKPEKTSFRKMFEIIRKNDQLLVAIATILTFNFGMNCIA
                     GVQTYYFIYVAGNKGLFSVFTMFAGFAEIFGLIIFPKLSQRLSKQQVYALASGIPVVG
                     LIILLVTGFIAPQNYILTAVAGICVKFGSGLQLGTVTVVLADVVDYGEYKLGTRNESV
                     IFSIQTLLVKFASAMGALFTGFALDATGYVAGASQTMATQNGMRIIMVALPIILVLIS
                     YIIYKKYYKLNGAYYQRIMNIIALRKEENQMILDDVTEDIKNSNEPIIDLKEKKYAN"
     gene            complement(1438..1569)
                     /locus_tag="PLK68_11815"
     CDS             complement(1438..1569)
                     /locus_tag="PLK68_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91133.1"
                     /translation="MNNDRLSFKEKYSYGVGAIGKDMCCGIIFTYCMLYFTDVQLHL"
     gene            complement(1574..1843)
                     /locus_tag="PLK68_11820"
     CDS             complement(1574..1843)
                     /locus_tag="PLK68_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM91134.1"
                     /translation="MKGQGIEFLIHVGLDTNQLNGDGFKIFVDPDQVVKQGDILLTFD
                     SEILKKHRCIDATPFVFTNLNRKNITVNRYGMVNLNEPFIIVERG"
     gene            complement(1840..2040)
                     /locus_tag="PLK68_11825"
     CDS             complement(1840..2040)
                     /locus_tag="PLK68_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM91135.1"
                     /translation="MGVFKKEIQVNCPIEGKVYPLEYCPDEVFSKGMTGEGVVIFPTG
                     NRVVAPIDGKINFIFSSKQQLF"
BASE COUNT          856 a          438 c          259 g          821 t
ORIGIN      
        1 aaaattttca cgatctttga ttgctttacc ataataaagt tgtcctaatt gattattttt
       61 taagatttta aaaatataac ttatctcttg attatataaa tgaaatatct tagttgtttt
      121 atgaaattta attggcatat ttcttctcct ttaaatcaat aatcggttca tttgaatttt
      181 ttatatcttc cgttacatca tcaagaatca tctgattttc ttctttcctt aaagcgatta
      241 tattcataat tctttgatag tacgctccgt ttaatttgta atacttttta taaataatat
      301 aacttattag cactaaaata attggtaatg caaccataat aatacgcatt ccattttgag
      361 ttgccatagt ttgagaagct ccagcaacat atcctgtggc atctaaagca aaacctgtaa
      421 ataaagcacc catagctgaa gcaaatttta ctaataaggt ttgaattgaa aaaattacag
      481 actcattacg tgtccctagc ttatattctc cataatctac aacatcggct aatactactg
      541 tcactgtacc taattgtaat ccagaaccga atttgacaca aatccctgct actgctgtta
      601 aaatataatt ttgtggagca ataaatcctg ttactaataa aatgattaga ccaactactg
      661 gtattccact agctaaagca tacacctgtt gtttacttaa tctttgagat aactttggaa
      721 agatgatcaa accaaaaatc tctgcaaatc ctgcaaacat tgtaaaaaca gaaaataatc
      781 ccttattgcc tgcaacataa ataaaataat aagtttgaac cccagcaata caattcattc
      841 caaaattaaa tgttaaaatc gttgctattg ctactaacag ctgatcattt ttacgaatta
      901 tttcgaacat ctttctaaac gaagtctttt ctggtttctt tgcttctact cgatcagctg
      961 atttaacatt aactacagta attcccacag ttacaataaa aataatggca atgacaatag
     1021 caaaatttgt atatcccact tgagcatcac catttccaag gaaatccatg atttgtaatc
     1081 caaacccacc aactaacaga gaacccccaa tcgatgcaaa aattcttgga ataacagcta
     1141 cacgatctcg ttcttctgga tttgatgtta aattaggtaa cattgaccaa tatgggatat
     1201 ccatgacggt atatgtcatt ccccatacga tatacataat agcagcaaat atatacaaac
     1261 tagttcctga taatccccaa tcagtaaata aagcaactaa aataattgca ttaacaatag
     1321 ttccaattgc taaccaaggt ctaaatttac cccatctact atgggtgtta tcaacaatca
     1381 ttcccatacc taaatcatta acagcatccc aaaattttgc taagaaaaac aatgttccta
     1441 caaatgaagc tgaacatctg taaaatacaa catacaataa gtaaagataa ttccacagca
     1501 catatcctta ccaattgcgc ctactccata tgaatatttt tccttaaatg ataatctatc
     1561 attattcatt tttctatccc ctttcaacga taataaatgg ttcatttaaa ttaaccatac
     1621 catatctatt aacagttata tttttacgat ttaaatttgt aaaaacaaac ggtgttgcat
     1681 caatacaacg atgttttttt aatatttctg aatcaaaagt taacaaaata tcaccctgtt
     1741 taacaacttg atcaggatct acaaatattt taaagccatc cccgtttagt tgattcgtgt
     1801 ccaaacccac atgaattaga aactctattc cttgaccttt caaaacaatt gctgctttga
     1861 agaaaaaata aaatttattt taccatcaat tggtgcgaca actctattac cagttggaaa
     1921 tattacaacc ccttctccag tcattccttt tgaaaaaact tcatcaggac aatactctaa
     1981 aggataaact tttccttcta tcggacaatt aacttgaatt tcctttttaa atactcccat
     2041 cactttcctc ctcagtttac tgtaagcgct tctatatcga caattataac atttggatat
     2101 atttataata gtattagatg tattttaaat atgtcaattt gttatatatt ccctaattac
     2161 aaaaggaaag taattgaaat aaatttattt attaatatta tatattttca aaatacaaca
     2221 ttaagacata tttttataac attttaacta aaattataaa gtagcttttt atttttttac
     2281 ctttaacttt aagattcatt gtattagaat aaaccacgag gtgaattatt aaatggaatt
     2341 ttcaaaacga atggaacttt ttcaagaaag tatt
//
LOCUS       DAOMTA010000485         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2083_length_2373_cov_2.936152, whole genome shotgun sequence.
ACCESSION   DAOMTA010000485 DAOMTA010000000
VERSION     DAOMTA010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_2373_cov_2.936152"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2373
                     /locus_tag="PLK68_11830"
     CDS             <1..>2373
                     /locus_tag="PLK68_11830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM91136.1"
                     /translation="AAIASDALVSYEINIDDDLIIEDIIENTVNMLKLVDLNTNCSYS
                     EFLLRWTKKCVHPDDKNKFYQELHPHRLKKLFEQGITEVYCEYRSLNATQKQGWVSTT
                     IHLLHVGETNKLFGFVYVKDINDKKIHELELLRQSQSDPLTKLYNRTAFGQIVNEYLN
                     KKRESSSALLLIDIDNFKNINDNLGHSFGDTVLCEIAHKLTNIFNNKAIIGRYGGDEF
                     IIFIKDIPSKKYVYHKASIILEELHLYYSSNYQEYTISSSIGITFSPDDGKTLHELFE
                     QTDSALYRAKKLGKSQYFAFNDSYQDITPVTNYISKGWLIDELDEIVYVSSLDTYELL
                     YLNRKGREITGIEAGEYNHIKCYEALQGRTSPCPFCTNAKLNLNEFYIWEFSNQHLNK
                     DYIVKDKLVLWEGTPVRMEIAVDVSDTHNFNLQRVPTEFAIEKTILDCLQALTIPDTL
                     EEAINNVLEIIGNFYQATRAYIVEIDLNTKIGSNTYEWCRENYPHYRDQLQRIDLNEI
                     PYIYEAFEHHNNLIINDCEAIKKEHPREYAHFISREAHSLVTIPYEETGIFAGYIGVD
                     NPSINQNTIALLDSINFSIVNEIKKRRLYEKTQYNLYHDNLSGLLNRNSFTQFLSYEN
                     SAVYSQGVILADINGLKEINRDFGHYHGDKIITIISSIMNSYFPSEKIFRLSGDEFII
                     IVNNLEYKQFIEATKQMEDTLLGSTPNGVSLGYTWSEDNMDINDLIHQAEELMMINKQ
                     IYYERADTYKKHYSPKKLENLLKCFKAKQFVVYLQPKFDIDQNKVVSAEA"
BASE COUNT          850 a          377 c          378 g          768 t
ORIGIN      
        1 agcagcaata gcatcagatg ctttagtatc ctatgaaatt aatattgatg atgatttaat
       61 tatcgaagat attatcgaaa ataccgttaa catgttaaaa ctagttgact taaacactaa
      121 ctgtagttat agtgagtttt tacttcgctg gactaaaaaa tgcgtccatc ctgatgataa
      181 aaataaattc taccaagaat tacatcctca ccgtttaaag aaattatttg aacaggggat
      241 tacggaagta tattgtgaat atcgttcgct caatgcaact caaaaacaag gctgggtcag
      301 tacaacgatt catctcttac acgttggtga aacaaataaa ttatttggct ttgtctatgt
      361 aaaagatatt aatgataaga aaatacatga attagaatta ttgcgccaat ctcaatccga
      421 tccgctcact aaactataca atcgaaccgc ttttggtcaa attgttaatg aatatttaaa
      481 taaaaaaaga gagtctagta gtgctttatt attgattgat attgataact ttaaaaatat
      541 caacgataat cttggtcata gttttgggga taccgtttta tgtgagattg ctcataagct
      601 aacaaatatt tttaataaca aagcaattat tgggcgttat ggcggcgatg aattcattat
      661 tttcattaaa gatattccat ccaagaaata tgtttaccat aaagctagta tcattcttga
      721 agaacttcat ctttattact catcaaatta tcaagaatat acgatcagca gttcaattgg
      781 tattactttt agccctgatg acggtaaaac attacacgaa ttgtttgaac aaaccgattc
      841 tgctctctat cgagcaaaaa aactgggcaa atcgcaatat tttgctttta atgatagcta
      901 tcaggatata actcctgtaa ctaattatat tagtaaaggc tggctaattg atgaacttga
      961 tgaaattgtt tatgttagca gcctagatac ttatgaatta ctatacttaa atcgaaaggg
     1021 acgtgaaatc accggaattg aagcgggaga atataatcat attaaatgtt acgaagctct
     1081 acaaggtcga actagcccct gcccattttg tactaatgct aaattaaatc ttaatgaatt
     1141 ttatatttgg gagtttagca atcagcatct aaataaagac tatattgtta aagataaatt
     1201 agtattatgg gaaggaacac ctgtgcgaat ggaaattgct gttgatgttt ctgatactca
     1261 taacttcaat cttcaacgtg ttcctactga atttgcaatt gaaaaaacga ttttagattg
     1321 tcttcaagcc ttgacaattc cggacactct tgaagaagct attaacaacg ttttagagat
     1381 cattggcaat ttttaccaag caacaagagc atatatcgtt gaaattgacc taaatacaaa
     1441 aattggttca aacacttatg aatggtgccg agaaaactat cctcactatc gtgaccaact
     1501 acagcggatt gatttaaatg aaattcctta tatttatgaa gcttttgaac atcataataa
     1561 tctaattatc aatgattgtg aagccatcaa aaaagagcat cctcgagaat atgcacactt
     1621 tattagtcgt gaagcccatt ctttagtgac aattccctat gaggaaactg gcatttttgc
     1681 aggctatatt ggtgttgaca atccttcaat taaccaaaat acaattgcat tacttgattc
     1741 tatcaatttt tctatcgtca acgagataaa gaaacgtcga ttatatgaaa aaacacagta
     1801 caatctttat cacgataact taagcggctt acttaatcgt aatagtttta ctcaattttt
     1861 gagttatgaa aatagtgctg tttactctca aggagtcatt cttgcagata tcaacggctt
     1921 gaaagaaatt aatcgcgatt ttggacatta ccatggtgat aaaataatta cgattatttc
     1981 tagtattatg aattcatatt tcccaagcga aaaaatcttt agattaagtg gtgatgaatt
     2041 catcattatt gtaaacaatc ttgagtataa acaatttatt gaagctacta agcagatgga
     2101 agatacttta ctcggttcaa ctccaaatgg tgtctcactt ggatacacat ggagtgaaga
     2161 taacatggat attaatgatt tgattcacca agccgaagaa ttaatgatga tcaacaaaca
     2221 aatatactat gaacgagctg atacatataa aaaacactat tcacctaaaa aacttgaaaa
     2281 tcttctcaaa tgttttaaag caaaacaatt tgttgtttac ttacagccaa aatttgatat
     2341 tgatcaaaac aaagttgttt cggccgaagc ttt
//
LOCUS       DAOMTA010000486         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2085_length_2371_cov_2.136010, whole genome shotgun sequence.
ACCESSION   DAOMTA010000486 DAOMTA010000000
VERSION     DAOMTA010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_2371_cov_2.136010"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="PLK68_11835"
     CDS             <1..2147
                     /locus_tag="PLK68_11835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM91137.1"
                     /translation="GVSLFGISLFTFVFAGIRKDQLKNHLEAVVNNSPGDIFMMTPNH
                     IEFFSSNLSTSLGYCKEEYCQLTKRSYDLIDKRDIDYVNEAFEAAYREKRPLDVIYRV
                     QHKNGSIAYIQMIASYYEDKAGRPYFYGDFINITEVVNARKNNQLVIDNTMTVVFLIR
                     LRDAPPNFENPEINMISIGILEKYGYTKQSCIEAMKKGGNCGLINKNDYAAIKDDYIK
                     AVDNKEDYNAIYRFELEQTTLWMDSKTKYLNTENGYRTYLCVLNDVTSMKQKEEEIWL
                     SSQILDRVIKMANLNIWQYDYQTKQLILMNLERDSFIYQILDLPRNEDIIIDNYLNKI
                     NNIEVMNEQTKEKIREQLIAQTVQNKFSYELPLTLNSKIIWIKVVGETIYDYGDRPLK
                     LVGYFKDVTLEKSSFQKYLENAKTLEALQEKSFYELSINLSQNRIINTNIQEDDLSFI
                     NSSYSSYINSITEKIIQKEYQDKVKEVLGLEAILTRYQQGTTTNSVVYKRYVDGEYYW
                     MEATYHILDLQENKDIFCYLYVIDIDKQKRQELALKNKAEHDGLTGLYNRSKAINEIN
                     QILLSNEMTCGALLMLDMNDFKEINDNYGHAIGDKIITKTAKRLKEMFRNDDILCRLG
                     GDEFMILCRNIDEISIRMKLEDVTRQMKIPYLIEEYQIMAPLSIGFVMIPLYGTTFNN
                     LYKKADIALYKAKQDGLASYRMYHDDMNKKAL"
     gene            complement(2153..>2371)
                     /locus_tag="PLK68_11840"
     CDS             complement(2153..>2371)
                     /locus_tag="PLK68_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91138.1"
                     /translation="YDKIISSDEAKTISLNYFNFTESDISNLNIELDNDSSNIYYNIS
                     FTKDKHHYSINIEAVNGIPTNAKTTKSS"
BASE COUNT          890 a          275 c          429 g          777 t
ORIGIN      
        1 gtggagtgag cttatttgga ataagtcttt ttacatttgt ttttgctggt attcgaaaag
       61 atcaactaaa aaatcatctg gaggcagtag taaataattc gcctggagat atttttatga
      121 tgacacctaa tcatattgaa ttttttagca gtaatttatc aacatcgtta ggttattgta
      181 aagaagaata ttgtcagctg acaaagcgga gctatgattt aattgataaa cgtgatattg
      241 attacgttaa tgaagcattt gaggcagcct atagggaaaa aagaccatta gatgttatct
      301 atcgtgttca acataaaaat gggagtattg cttatattca aatgattgca agttattatg
      361 aagacaaggc tggaagacca tatttttatg gtgactttat taatattacc gaggtagtaa
      421 atgcacgaaa aaataatcag ttagttattg ataatactat gaccgtagtc tttttaattc
      481 gtttgagaga tgctcctcca aattttgaaa atccagaaat aaatatgatt tcaatcggta
      541 ttttagaaaa atatggatat acaaaacaat cgtgtatcga agcaatgaaa aaaggtggaa
      601 actgcggatt gatcaataaa aatgattatg cagcaattaa agatgactat atcaaagcgg
      661 tagataataa agaagattac aatgcgattt atcgatttga attggaacaa acaacattgt
      721 ggatggattc caaaacaaaa tatttaaata ctgaaaatgg ttatcgaaca tatttgtgtg
      781 tattaaatga tgtaacatca atgaaacaaa aagaagagga gatttggctg tcatcacaaa
      841 ttcttgatcg cgttataaaa atggcaaatc taaatatttg gcagtatgat tatcaaacaa
      901 agcagctgat tttaatgaat ttagagagag atagttttat ttatcaaata ttagatcttc
      961 caaggaatga agatattatt attgataatt atttaaataa aatcaataat attgaagtaa
     1021 tgaatgaaca aactaaagaa aaaataaggg aacagctgat tgctcaaaca gtacagaata
     1081 aatttagtta tgaattaccg cttacgctta atagtaaaat tatttggatc aaagtagtcg
     1141 gagaaactat ttatgattac ggtgaccgtc ctttgaaatt agtaggttat tttaaagatg
     1201 ttactcttga gaaatcatca tttcaaaaat atttagaaaa tgctaaaaca ttagaagcat
     1261 tacaagaaaa atctttttat gagttgtcaa ttaatttaag tcaaaatcga attattaata
     1321 ctaatataca ggaggatgat ttaagtttta taaacagcag ttatagcagc tatatcaata
     1381 gcataactga aaagattatt caaaaagaat atcaggataa agttaaagaa gtcttaggat
     1441 tagaggcaat tctcactcgc tatcaacaag gtactacaac taattctgtg gtgtataaac
     1501 gatatgttga tggtgagtat tattggatgg aggcaactta tcatattctt gatctgcagg
     1561 aaaataaaga tattttttgc tatttatatg tcattgatat tgataaacaa aaacggcaag
     1621 aattggcttt gaaaaataaa gcagaacatg atggtttgac aggattatat aatcggtcta
     1681 aagctattaa tgaaatcaat cagatcttgc ttagtaacga aatgacgtgc ggagcattat
     1741 tgatgctgga tatgaatgat tttaaagaaa ttaatgataa ttacggtcat gctattggtg
     1801 ataaaataat tactaaaact gctaaacgat taaaagagat gtttagaaat gatgatatct
     1861 tgtgtcgact cggaggcgat gaatttatga ttctatgtcg caatatcgat gaaatatcaa
     1921 tccgaatgaa attagaagat gtcacaagac aaatgaaaat accatatttg attgaggagt
     1981 accaaattat ggctccatta tcgattgggt ttgtgatgat ccctttgtat ggtacgacat
     2041 tcaataatct ttataagaag gccgatattg ctttatacaa agctaagcag gatggtttgg
     2101 ctagttatcg gatgtatcat gatgatatga ataaaaaagc tctttagtaa atctaagagc
     2161 tttttgttgt tttagcatta gtgggaatcc catttacagc ttcaatattg atggagtagt
     2221 gatgcttatc tttagtaaaa ctgatgttat agtagatatt gctactgtca ttatctaact
     2281 caatgtttaa gttactaata tctgattcag tgaaattaaa ataatttaag ctaattgttt
     2341 tagcttcatc actactgatg atcttatcat a
//
LOCUS       DAOMTA010000487         2361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2092_length_2361_cov_1.918474, whole genome shotgun sequence.
ACCESSION   DAOMTA010000487 DAOMTA010000000
VERSION     DAOMTA010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2092_length_2361_cov_1.918474"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLK68_11845"
     CDS             complement(<1..330)
                     /locus_tag="PLK68_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM91139.1"
                     /translation="MKVNSLCLDNFRNYNHFFIEFDRDINILIGSNGQGKTNLIEAIY
                     LLSVGKSFKTHINKQMIMFDCEFAKVKGEVTSNNKLRSLEMILGSDFKRAKIDDQDIY
                     KISEYVGL"
     gene            complement(330..536)
                     /gene="yaaA"
                     /locus_tag="PLK68_11850"
     CDS             complement(330..536)
                     /gene="yaaA"
                     /locus_tag="PLK68_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HRM91140.1"
                     /translation="MKKIQIRDEYITLGQFLKYAGCVSSGIEAKMVIKDELVMVNGEV
                     ETRRGKKLRTGDQIEFNGESFVID"
     gene            complement(746..1900)
                     /gene="dnaN"
                     /locus_tag="PLK68_11855"
     CDS             complement(746..1900)
                     /gene="dnaN"
                     /locus_tag="PLK68_11855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRM91141.1"
                     /translation="MNFKIKRLKLLNALAKATKAVSIRSPLPVLTGIKFDLQDDCLIL
                     TGSDSDITIQTKIEKDEDLTIYQTGGVVLNSRYILDIVRKIDSDEIKIEILDGALTRI
                     SGATSKFDLNGTDVLDYPRIDLSKTGTKVLLNALSLKDIISQTKFAASDKEHKPILTG
                     INFKAGNRQLECTATDSYRLAKKIVSLDEDVTFNITIPQKSLDEISKIIERDEMIEMY
                     VSDRKVLYVFDNNIIQTRLIDGTFPDVQRIIPTSCVSKMLVDSYEIAGTIDYELDLDA
                     HYLLNAIDRVSLLTNEQNNIIKLDMSDEKVVLSSYMQEIGSVEEILDKSFYKGTPISI
                     SFSSKYATDAIRAFNEPKIKILFTGEMKPFIIKDFEKDDVIQLVLPVRTF"
     gene            complement(2056..>2361)
                     /locus_tag="PLK68_11860"
     CDS             complement(2056..>2361)
                     /locus_tag="PLK68_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91142.1"
                     /translation="QNPKSSLTKESILKTTAEFYYLTISQLISKNKTRKLTTPREMCM
                     YLMRELLDITFAEIGATFSNRDHSTVMKACARVETKIKKDPDYKLAINKLKDKLGIV"
BASE COUNT          806 a          372 c          280 g          903 t
ORIGIN      
        1 aagaccaaca tattcactaa ttttataaat atcctggtca tctattttcg cacgtttaaa
       61 atcactccca agaatcattt ctaaactacg gagcttatta ttggaagtga cttcaccttt
      121 tactttcgca aattcgcaat caaacataat catctgctta tttatatgag ttttaaaaga
      181 ttttcctact gacaataaat aaatcgcttc gatcagatta gttttacctt gtccattact
      241 gccaattaaa atattgatat cacgatcaaa ctcaataaaa aaatgattat aattacgaaa
      301 attgtctaga cataatgaat taactttcat taatcaataa caaaagactc tccattaaat
      361 tctatctggt caccagttcg aagtttcttt cctcttcttg tttccacttc accattaacc
      421 atcactaatt catctttaat aaccatctta gcttcgattc cactagaaac acatcctgca
      481 tactttaaaa actgtcctag ggtaatatat tcatctctta tttgaatttt tttcatgact
      541 atcctctttt tttataaaat ctagagttat tataacataa attgttgtta aaatcaaaaa
      601 aataagcatt ttaacgcatt ttttataaaa atgaataaaa tcttattttt tattaaaatt
      661 cgctcatatt taaaatttat ccacatataa ataaaacaac tagtaaaaaa atactttcac
      721 gaaaaaaaaa gctacataat gtagcttaga aagttcttac tggtaaaact aattgaatta
      781 catcatcttt ttcaaaatct ttaataataa aaggtttcat ttctccagta aataaaattt
      841 taatttttgg ttcattaaaa gctctaattg catcagttgc atattttgaa ctaaatgaga
      901 tacttattgg tgttccttta taaaaagatt tatctaaaat ttcttctaca gaaccgattt
      961 cttgcatata tgatgatagg acgacttttt cgtcagacat atctaattta ataatattat
     1021 tttgttcatt tgttagtaat gaaacacgat caatagcatt taatagataa tgagcatcta
     1081 aatctagttc ataatcaatt gtacctgcaa tttcatatga gtctactaac atcttcgaaa
     1141 cacagcttgt tggaatgatt ctttgtacat ctgggaaagt tccatcaatt aatcgtgttt
     1201 gaataatatt attatcaaac acataaagga ctttacgatc agaaacatac atttcaatca
     1261 tttcatcacg ttcaataatt tttgaaattt catctaaaga tttttgagga attgtaatat
     1321 tgaaagtaac gtcttcatct aaagatacaa ttttcttagc tagacgatag ctatccgttg
     1381 ctgtacattc taattgacga tttcctgctt taaagttgat tcctgttaaa attggtttgt
     1441 gctctttatc actagcagca aatttggtct gtgaaataat atcttttaaa gatagtgcat
     1501 ttaataatac ttttgttcca gtttttgata aatcaattcg tggataatct aaaacatcag
     1561 taccatttaa gtcaaattta gaagttgctc ccgaaattct agttaaagca ccatctaaaa
     1621 tctcaatttt aatttcatca ctatcaatct tacgaacaat atctaagata taacgagaat
     1681 ttaaaactac cccaccagtt tgataaatcg ttaaatcttc atctttttca attttagttt
     1741 gaattgtaat gtcagaatca ctaccagtta gaattaaaca atcgtcttgt aaatcaaact
     1801 taattccagt caatactggt aagggacttc gaattgaaac tgctttagtt gctttagcaa
     1861 gagcatttaa taatttaagt cgttttattt taaaattcat aaatttcctc cttgtgacta
     1921 attatatata attatttaaa ataatgctat taatattatt atagtgtgaa tattgtgcat
     1981 aagcattaaa taagctgata taatcagtgt tttttatgtg tataactatg tgtgcaattt
     2041 gtggtagttt tgtgtctaaa cgatacctaa tttgtctttt aacttattta ttgctaattt
     2101 atagtcagga tctttcttga ttttagtttc tacgcgagca catgctttca ttactgttga
     2161 atgatctcga ttgctaaaag tagccccaat ttcagcaaaa gtaatatcta ataattcccg
     2221 cattaaatac atacacattt ctcttggggt tgttaattta cgtgtcttgt tttttgaaat
     2281 tagctgactg attgtcagat aatagaattc tgcagttgtt tttaaaatac tttctttagt
     2341 tagggaactc tttggatttt g
//
LOCUS       DAOMTA010000488         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2093_length_2360_cov_2.902386, whole genome shotgun sequence.
ACCESSION   DAOMTA010000488 DAOMTA010000000
VERSION     DAOMTA010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2093_length_2360_cov_2.902386"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLK68_11865"
     CDS             <1..451
                     /locus_tag="PLK68_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRM91143.1"
                     /translation="ELLNYYHSYNHSHHEDFVYVYPTVVETLEYLKNRGYPLGIVTTK
                     LKVAADVGLNTFDLKKYFDVVIGLDDVKVTKPDPEGIIKAMELLGVKKAVYIGDNITD
                     IQAGKNAGIKTIGVKWSPKGYQHLLELEPDLMIDEMKEIIPYIEGAC"
     gene            453..1361
                     /gene="trxB"
                     /locus_tag="PLK68_11870"
     CDS             453..1361
                     /gene="trxB"
                     /locus_tag="PLK68_11870"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRM91144.1"
                     /translation="MKDLIIIGAGPAGLSACLYATRAGAEVLMLDAGAPGGKLNVSAE
                     IENWPGQKKKTGPELAYEMYEHALSFGGEQAYGEVTKVIDYGDYKEVITTDQSYEAKN
                     VLIATGTKERKMGIPMEEELTGHGVSYCAVCDGPFFKGDEVAVVGGGNSALEEAIYLT
                     KFAKKVHLIVRRDVFRADKIIQDHVQANDKIEIHFLKKPHHLIAKDNKVAAIALEDSK
                     TGEVSELAVKGVFPFVGLDPITDFVKDLGITDERGYIITNELMETSVKGIYAAGDVRQ
                     KVLRQVVTATNDGAIVGQQVGQGLNN"
     gene            complement(1358..1942)
                     /locus_tag="PLK68_11875"
     CDS             complement(1358..1942)
                     /locus_tag="PLK68_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRM91145.1"
                     /translation="MTIGSTLISPILKKLKDKYQDITFQVYIDNTNVIEEKLLNNTLD
                     IALVEGTIINQDLIVEPVIKDRVVFVCAQDYPLNNVKVMSLTELSLHPFIDREQGSGT
                     RNQLDECLKNNNIFLEPAWQCHSWESVKQAVLNGHGIALMPMKIIENEVIEKSIQVIN
                     VNNISIERDFSICYHKNKSINKKMQAFIKTCKNY"
     gene            1936..>2360
                     /locus_tag="PLK68_11880"
     CDS             1936..>2360
                     /locus_tag="PLK68_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase"
                     /protein_id="HRM91146.1"
                     /translation="MSKFIDAIFTNKLITTFEDIQICATSSMASLKAMKVVETENLLL
                     GISTKGQYPAFTKKLAYELEQYDDYLSALSNIRRLVLLNNLVNKHDRKQFFKQLMYFE
                     KTELDMLLMFILEKEGRLSIFTAQCMNVIDYFAEQVSAMS"
BASE COUNT          812 a          303 c          456 g          789 t
ORIGIN      
        1 tgaattgttg aattattatc atagttataa ccatagtcat cacgaggatt ttgtctatgt
       61 ttatccgaca gtagtagaaa cattagaata tttaaaaaat agaggttatc ctttaggaat
      121 cgttacgact aaattaaaag ttgctgctga tgtaggactc aatacttttg atttaaaaaa
      181 atattttgat gtagtaattg gattagacga tgttaaggtc actaagccgg atccagaagg
      241 aataatcaag gcaatggaat tattgggtgt taaaaaagct gtatatatcg gtgataatat
      301 aactgatatt caagctggga aaaatgctgg tattaaaaca attggtgtaa aatggtcacc
      361 taaaggttat caacatttat tagaattaga accagattta atgatcgacg agatgaagga
      421 aatcattcca tatatagaag gagcgtgtta agatgaaaga tttaattatt attggagctg
      481 gtcctgctgg attaagtgca tgtttatatg caactagggc aggggcagag gtgttgatgc
      541 tggatgctgg agcacctgga ggaaagttga atgtcagtgc tgaaatcgaa aactggcctg
      601 gacaaaaaaa gaagactggc cctgagttag cttatgaaat gtatgaacat gcattatcat
      661 ttggcggtga acaggcatat ggtgaagtaa ctaaagtaat tgattacggt gattataaag
      721 aagttataac aacggatcag tcatatgaag caaaaaatgt cttgattgct actggaacta
      781 aggaacgtaa aatgggaatt ccgatggaag aagaattaac cgggcatggt gtttcttatt
      841 gtgctgtttg tgatggacca ttttttaaag gtgatgaagt agcagttgtt ggtggtggta
      901 attcggcatt agaagaagct atttatttaa ctaaatttgc taaaaaagta catttaattg
      961 ttcggcggga tgtttttaga gctgataaaa taattcaaga tcatgttcaa gctaatgata
     1021 aaattgaaat tcattttcta aaaaaacctc atcatcttat tgcaaaagat aataaggttg
     1081 cggcgattgc attagaagat agtaagactg gtgaagttag tgaattagca gttaagggtg
     1141 tctttccatt tgtaggatta gatccaatta ctgattttgt taaagactta ggaattactg
     1201 atgagcgtgg ctatatcatt acaaatgaat tgatggaaac atctgtcaaa gggatttatg
     1261 ctgctggtga tgttcgtcaa aaagtacttc gccaagttgt gacagctacc aatgatggtg
     1321 caattgttgg tcaacaagtt ggtcaaggct taaataatta ataatttttg caggtcttaa
     1381 taaaggcttg cattttttta tttatacttt tatttttatg ataacaaatt gaaaaatcgc
     1441 gttcaatact gatgttattg acattaataa cttgaatact tttttcaata acttcatttt
     1501 caataatttt cataggcatt aaggcgatac catgaccatt taaaactgcc tgtttaacag
     1561 attcccaact atgacattgc caagcaggct ccaaaaaaat attattattt tttaagcatt
     1621 catcaagttg atttcttgtc ccactacctt gttcacgatc aataaaagga tgtaaactca
     1681 attcagttag gctcattact ttaacgttat ttagagggta atcttgggca caaacaaaaa
     1741 caactcgatc tttgataacg ggttcgacaa ttaaatcttg attgatgatc gtaccttcaa
     1801 ctaaagcaat atctaaagta ttatttaata atttttcttc aataacatta gtattatcaa
     1861 tataaacttg aaaagtaata tcttgatatt tatcttttaa tttctttaaa ataggtgaaa
     1921 taagagtact gccgattgtc aaagtttatt gacgctattt tcaccaataa attaattact
     1981 acttttgaag atattcagat ttgtgctact tcaagtatgg catctttaaa ggccatgaag
     2041 gtagtagaaa cggaaaatct gcttttaggt atatcaacaa aggggcagta tccagctttt
     2101 actaaaaaat tagcttatga attagaacaa tatgatgatt atttatctgc tttaagtaat
     2161 attcgtaggc tggttctttt aaataatttg gtaaataaac atgatcgaaa acagtttttt
     2221 aagcagctga tgtattttga aaaaacagaa ttagatatgt tactaatgtt tattttagaa
     2281 aaagaaggac gtctatcaat ttttacagca cagtgtatga atgtgattga ttactttgct
     2341 gaacaagtat ctgcgatgtc
//
LOCUS       DAOMTA010000489         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2100_length_2356_cov_1.982182, whole genome shotgun sequence.
ACCESSION   DAOMTA010000489 DAOMTA010000000
VERSION     DAOMTA010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_2356_cov_1.982182"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1057..2295)
                     /locus_tag="PLK68_11885"
     CDS             complement(1057..2295)
                     /locus_tag="PLK68_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91147.1"
                     /translation="MKKIFQFFIAVVMCLSLSTNIYAINDNEHEITTDKPHIILTPNQ
                     AGDELTVKLHLSQEMFERQIATMHLSINTSKSIQEYQNAFMLDEGLKKLASIYDYNLS
                     ENKIDLLLSNKTKLFEQQEITLGTIRIDSNKDFEIEFTPAKITYVYVNDKAENSIEFD
                     CPAVILSSVSGGDSGDNGQDPADKITVETVVEHLGGSKLELAEESQKVVQTSVMSYLN
                     KHYAEILGNLPEGTVIKAQLSLKNLTESDITIDQKDKIEKVLEDGAKILAYYDISITA
                     TAYSKDDVIIPEINGVEISEMASPIKLSMTIPSEFIKKNRQFGIVRLHDNEANGLKSS
                     LANENIITFETDRFSIYTLVAKDYTQAEVNKDPDVLNNSIVKTGDDTSSMLPMIFMAA
                     SLLVIIGILEMKRREVNKQK"
BASE COUNT          798 a          376 c          287 g          895 t
ORIGIN      
        1 attaaaacaa ttggctgttt agtaagtcac aaacgaattt tagttgaatt tattattagt
       61 attttgggtc tggatctact ctatgccatt ttttcaaaca cattcaaaaa gcttaatttt
      121 ttatctacaa aatccagaat tgtattcaca gaccattatt tttatgatga ttgcaataat
      181 cattgtcact gccggttacg gaaatcgaca aattattgtc attgataaaa aatattttaa
      241 atattgcagt aatcaaaatt tatggtttaa ttaccaacaa ttttaccgca ttatttttaa
      301 ctgggaacca acatttgaca ttcaaatact gctagttaat attgcaaaga ttgccctatc
      361 atacagtgat atttatatac tatgcaatca aatttattga ggctcaaggc gttaaaatcc
      421 aggatcccta tcacttaaaa gaagctttag ctaataaaga catgcgtttt gctgaatata
      481 ttgaaaaaac aagtaatcat ttttaaagaa attacatcta ttcatgacct gaaaaaaatt
      541 tttctccacc caaaatggat agccactcaa ttatatttat cattgataat caagaattag
      601 tgttcctttc attaagttac catcctttat gctttattgg aaaaaaatga tttagaaaca
      661 gagcttaatc acgctatttc tttgttatta aacctgcata aaaatattga acaaatttta
      721 acttttaaaa ttgggtattc ttatattcaa ataaaaatca atcaaataat ttggattgaa
      781 tcattagtta ctatttaatt atccacactc agcttggtca atataaattt cgaaaaaact
      841 tagtcaatta ctcgatgaat tagctgttaa tcaatttatt caagttcaca agagctatat
      901 tgttaatcaa aaccacattg ttattcgaaa aagtaatgaa atagttttaa aaaataaaat
      961 agctattccg attggtcgga aatttaaaaa tactaattaa aataaaaaag agaaatagaa
     1021 atgcctattt agcataattt ctctttttcg ttataactac ttttgtttat ttacttctct
     1081 tcttttcatt tctaaaattc caataatcac taacaatgaa gctgccataa aaatcattgg
     1141 taacattgat gaagtgtcat caccagtttt aacaatagaa ttatttaata catctggatc
     1201 tttatttact tctgcttgtg tatagtcctt agcgactaat gtatagattg aaaaacgatc
     1261 agtctcaaaa gtaataatat tttcatttgc taagctagat tttaagccat tagcttcatt
     1321 atcgtgcagt ctaactattc caaattgacg gtttttctta ataaattcac taggaattgt
     1381 catagataat ttgattggag acgccatttc actaatttct acaccattta tttctgggat
     1441 gatcacgtca tcttttgaat aagctgtagc cgtaatagaa atatcgtaat aagctaatat
     1501 tttcgctcca tcctctaaaa ctttttcaat cttatctttc tgatctatcg ttatgtcact
     1561 ttctgttaga tttttcaatg aaagttgagc tttaataaca gttccctcag gtaaatttcc
     1621 taaaatttct gcatagtgtt tatttaaata actcatcacg cttgtttgaa ctactttttg
     1681 cgattcttca gcaagctcta atttactccc acctaagtgc tcaaccacag tttctacagt
     1741 tattttatca gcaggatctt gtccattatc tcctgagtca ccaccagaca cacttgataa
     1801 tattactgct ggacaatcga attctatact attttcagct ttatcattta catatacata
     1861 tgtaattttt gctggtgtaa attctatttc aaaatcttta ttactatcaa ttctaattgt
     1921 tcctaaagta atctcttgct gttcaaataa cttagtttta ttcgataata acaaatcaat
     1981 tttattttcg gaaagattat aatcatatat tgaagctagt ttctttaatc cttcgtccaa
     2041 cataaatgca ttttgatatt cttgaattga ttttgacgta ttaatagata aatgcatcgt
     2101 tgcaatttga cgttcaaaca tttcttgact taaatgtagt tttactgtca actcatcgcc
     2161 agcttgatta ggtgtcaaaa taatatgtgg cttatctgtt gtaatttcgt gttcattatc
     2221 attaatagca taaatattag ttgataaaga taaacacatt actacagcta taaaaaattg
     2281 aaatattttt ttcattattt accacctctc ttttgatttc cgattgtgat tgaaggtaat
     2341 tcagttggaa ttttca
//
LOCUS       DAOMTA010000490         2352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2107_length_2352_cov_2.370483, whole genome shotgun sequence.
ACCESSION   DAOMTA010000490 DAOMTA010000000
VERSION     DAOMTA010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2107_length_2352_cov_2.370483"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLK68_11890"
     CDS             complement(<1..353)
                     /locus_tag="PLK68_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91148.1"
                     /translation="MKLFSLALRNIKKSIKDYSIYFITLVIAVAIFYIFNSIDSQTAM
                     MSISKSTMEIVQSLVNVLSYISVFVSVVLGFLIVYANNFLIKRRKKEIGIYMTLGMSK
                     LKVSTILVLETVIVGV"
     gene            complement(340..1107)
                     /locus_tag="PLK68_11895"
     CDS             complement(340..1107)
                     /locus_tag="PLK68_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91149.1"
                     /translation="MEKILRIEKIEKYYGNKNNITKAVDNISFDVNGGEFVAIMGASG
                     SGKSTLLNCISTIDKVTSGHIYLKDADITKLKGYKLTKFRRDSLGFIFQDFNLLDTLT
                     SFENIALALTIQKSDYKKVDEKVNKVAAALGIKEILSKYPYELSGGQKQRVACARAIV
                     TNPDLILADEPTGALDSKSARMLLDSLNALNEKLNATILMVTHDSFTASYADRVIFIK
                     DGKIFNEIVKGDNSRKMFFNSIMDVQTLLGGELNEVI"
     gene            complement(1181..2188)
                     /locus_tag="PLK68_11900"
     CDS             complement(1181..2188)
                     /locus_tag="PLK68_11900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM91150.1"
                     /translation="MRLFDYLKDEIITVTVMISVTCLVMLILAVFNCPTFLVVYIPLL
                     LISSFVVVLMYNFFRRRNFYNNLIGILDHLDEKYLITELIGNPHFLDGKIMVNCIYEI
                     DKSMKEHLNDAIYRQSELKEYIEMWCHEVKTPVATSQMIIENNLNPVNESIKEELIKI
                     DNYVEQVLFYARSENVEKDYLIKDVKLSELVNSVIKRNKKDLINKHVKIELENLEIDV
                     ASDKKWLEFILNQIINNAIKYVDETPIIKLFAKQYKDRVELMIKDNGIGILENELNRV
                     FDKGFTGTTGRTKQKSTGIGLYLCKKLCYRLNHEIKIDSNSHGTVVTLVFPYSSHITL
                     Q"
     gene            complement(2185..>2352)
                     /locus_tag="PLK68_11905"
     CDS             complement(2185..>2352)
                     /locus_tag="PLK68_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM91151.1"
                     /translation="KIKSREELMAYLWDSDMFVDDNTLTVNINRLRKKLEDIGLNDVI
                     HTRRGQGYIIL"
BASE COUNT          835 a          419 c          229 g          869 t
ORIGIN      
        1 actccaacaa taactgtttc aagtactaaa atcgtagata cttttaactt tgacattcct
       61 aaagtcatat aaataccgat ttcttttttt cggcgtttaa ttaagaaatt atttgcataa
      121 acaattaaaa atcctaaaac taccgaaaca aaaaccgaaa tatatgataa aacatttact
      181 aaagactgca caatttccat tgttgatttg ctaattgaca tcattgctgt ttgactatca
      241 atcgaattaa agatataaaa tatagcaaca gcaataacta gtgtaataaa ataaattgag
      301 taatctttta tactcttttt aatatttcta agtgctaaac taaataactt catttagttc
      361 accacctaat aatgtttgaa catccataat actattaaag aacattttac gagaattatc
      421 acctttaaca atttcgttaa agattttacc atctttgata aagattactc gatcagcata
      481 actagctgta aatgaatcat gagtaaccat caaaattgtt gcattcaatt tttcatttaa
      541 cgcattcaaa ctatctaata acattcttgc tgatttacta tcaagagctc cagttggttc
      601 atccgctaaa atcaaatcag gattagtaac gatagctcta gcacaagcaa ctctttgttt
      661 ttgaccacca gataattcat atggatactt tgataaaatt tctttgattc ctaaagctgc
      721 tgcaaccttg tttactttct catctacttt tttataatct gatttttgaa tcgttaaagc
      781 caaagcaata ttttcaaatg aagtcaatgt atctaaaaga ttaaaatctt gaaagataaa
      841 gcctaacgaa tcccttctaa actttgttaa tttatatccc tttagtttag taatgtcagc
      901 atcctttaaa taaatatgac cactagtaac tttatcaatc gttgaaatac aatttagtaa
      961 agtacttttc ccactaccac tagcccccat aatggctaca aactcaccac cgttaacatc
     1021 gaaactaata ttatcgacag cctttgtaat gttgttttta tttccataat atttttcaat
     1081 tttttcaatt cttaaaattt tttccatttc tcttcacctc aacaactatt atagccgtca
     1141 taataatttc taccatcgaa taaaataaca taaccttaca ttattgtaag gttatgtgtg
     1201 agctgtacgg aaaaaccaaa gttactaccg taccatgtga atttgaatct attttaattt
     1261 catgatttaa tcgataacat aattttttac ataaatataa accaattccc gtactctttt
     1321 gtttagttcg tcccgttgtt cccgtaaatc ctttatcaaa aacacgattc aattcatttt
     1381 ctaagattcc aattccatta tcttttatca ttaattcaac acgatcttta tattgttttg
     1441 caaatagttt aattattggt gtttcatcaa catatttaat tgcattatta ataatctgat
     1501 ttaaaatgaa ctctaaccat tttttatcac tagcaacatc tatttcaaga ttctctaatt
     1561 caattttaac atgtttattg attaaatctt ttttattacg tttaattact gaattaacta
     1621 attcactaag tttaacatct ttaattaaat agtctttttc aacattctca cttcgagcat
     1681 aaaagagtac ttgttcaaca taattatcta tctttatgag ttcttcttta atactctcat
     1741 taactggatt tagattattt tcaataatca tttgacttgt agctacaggt gtttttactt
     1801 catgacacca catctcaata tactctttga gttcactttg tcgataaatt gcatcattta
     1861 aatgttcttt cattgattta tcaatttcat aaatacagtt aaccataatt ttaccatcta
     1921 gaaaatgagg atttccaatc aattcagtaa taagatactt ttcatcaaga tgatctaaga
     1981 ttcctatcaa attattataa aaatttctac gccgaaaaaa attatacatt aatactacta
     2041 caaatgagct aatgagcaga agtggaatgt agactactaa aaaagttgga caattaaaaa
     2101 ctgccaaaat caacattacc aaacaagtca cagaaatcat tactgtaacc gtaatgattt
     2161 catcttttaa ataatcaaat aatctcataa aatgatatac ccctgaccac gtctagtatg
     2221 aattacatca tttaaaccaa tatcctctaa ctttttacgt aagcgattaa tattaacagt
     2281 caatgtatta tcatccacaa acatatcact atcccataaa tatgccatta attcttctcg
     2341 agatttaatt tt
//
LOCUS       DAOMTA010000491         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2124_length_2342_cov_2.783559, whole genome shotgun sequence.
ACCESSION   DAOMTA010000491 DAOMTA010000000
VERSION     DAOMTA010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_2342_cov_2.783559"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..733
                     /gene="rpsL"
                     /locus_tag="PLK68_11910"
     CDS             320..733
                     /gene="rpsL"
                     /locus_tag="PLK68_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRM91152.1"
                     /translation="MPTINQLVRQGRNDKTTKSKSPALNRGFNSLAKKPTTTNSPQKR
                     GVCTRVATMTPKKPNSALRKYARVRLSNGMEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGTMDCAGVNDRKQGRSRYGAKKPKA"
     gene            762..1232
                     /gene="rpsG"
                     /locus_tag="PLK68_11915"
     CDS             762..1232
                     /gene="rpsG"
                     /locus_tag="PLK68_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRM91153.1"
                     /translation="MARKGQVAKRDVLPDPVYNSKTVSKLINNIMLDGKKGVAQNILY
                     DAFKKVEEKTGNPAMEVFDQAINNIMPVLELKVRRIGGANYQVPVEVSGERRMTLGLR
                     WLVNYSRLRNENTMVDRLANEIIDASNGAGASVKKKEDTHKMAEANKAFAHFRW"
     gene            1266..>2342
                     /locus_tag="PLK68_11920"
     CDS             1266..>2342
                     /locus_tag="PLK68_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM91154.1"
                     /translation="MSREFSLEKTRNIGIMAHIDAGKTTTTERVLYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNGYRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDSKAGVEPQTETVWRQATTYGVPRIVFCNKMDATGADFIMSLESLEKRLGVHGV
                     ALQLPIGAEDTFEGIIDLIKMKAVYFEGTKGENVIYKDIPEEYLDQANEYHAKMLDAA
                     ASYDDDLMMKVLEEEEPTEEEVKAAIRKGVLAVELFPVLCGSAYKDKGVQPMLDAVID
                     FLPAPTDIPSIKGIDEDGNEIEKHASDEEPFAALAFKIMADPFVGRLTFFRVYSGTMN
                     SGSYVLNATKNKKERVGRILQMHAN"
BASE COUNT          845 a          351 c          468 g          678 t
ORIGIN      
        1 gaacgaattt taatggttgt taagacaaga gatgatttag aagcatttat gaatacttta
       61 aagcgttatt tagattcagt agatataaaa ttaaagaaag agtaaaaact gtttaaatat
      121 aaaaagcgtt taaatggttt ttattttgtt tattaatgat ataatcaatt ttttaaaaaa
      181 aaaacaaata aaattattga catatttata tttttgtaat aatatgaact acggtgcccg
      241 cataggggcg cgttgtttat tacgaaatat gaaacacccg gaatcgtgtg tttaagtatg
      301 gaagaaagga gaacaaggaa tgcctacaat taatcaatta gttagacaag gtcgtaacga
      361 caaaactaca aaatcaaaat caccagcgtt aaacagaggt tttaactcat tagctaagaa
      421 accaacaaca actaattcac ctcaaaaacg tggtgtttgt acacgtgttg ctactatgac
      481 acctaaaaaa cctaactcgg ctttacgtaa atatgcccgt gttagattat caaatggaat
      541 ggaagtaact gcatatatcc caggaattgg acacaactta caagaacata gtgttgtttt
      601 aattcgtggt ggtcgtgtta aagatttacc aggggttcgt tatcacatcg tacgtggtac
      661 aatggactgt gctggtgtta atgatcgtaa acaaggccgt tcaagatacg gtgctaaaaa
      721 accaaaagct taagaaaaat caagtatagg aggaaaatta aatggcaaga aaaggtcaag
      781 ttgctaaaag agacgttcta cctgatccag tgtacaattc taaaactgta tctaaattaa
      841 tcaacaacat tatgcttgat ggtaaaaaag gtgttgctca aaacatctta tatgatgcat
      901 ttaaaaaagt agaagagaaa actggaaacc cagcaatgga agtttttgat caagcaatca
      961 acaacattat gccagttctt gaacttaaag ttagacgtat tggtggggct aactaccaag
     1021 taccagtaga agtatctggt gaaagaagaa tgactttagg gttaagatgg ttagtaaatt
     1081 attctcgttt aagaaacgaa aatacaatgg ttgatcgttt agctaacgaa attattgatg
     1141 cttctaacgg agctggtgct tctgttaaga agaaagaaga tactcataaa atggcagaag
     1201 ctaataaagc atttgctcat ttccgttggt aatatccaac aaatgataac gaaaggagaa
     1261 aaattatgtc tcgtgaattc tcattagaga aaactcgtaa tatcggaatc atggctcata
     1321 tcgatgctgg taaaacaaca actactgaac gtgtccttta ttacacaggt aagattcata
     1381 aaattggaga aacacatgaa ggtgcttctc aaatggactg gatggaacaa gaacaagaac
     1441 gtggtattac aatcacttct gcagcaacaa ctgcacaatg gaatggatac cgtgttaata
     1501 tcatcgatac accgggccat gtagacttca ctgtagaagt tgaacgttca ttacgtgtat
     1561 tagatggtgc ggttactgta ttagactcaa aagcaggtgt tgaacctcaa actgaaacag
     1621 tttggcgtca agcaactact tatggagtac cacgtattgt attctgtaat aaaatggatg
     1681 ctactggtgc tgacttcatt atgtctttag aatctttaga aaaaagatta ggggtacatg
     1741 gtgttgcact tcaattacca atcggtgctg aagatacttt cgaaggaatc atcgacttaa
     1801 ttaagatgaa agctgtttac ttcgaaggga ctaaaggtga aaacgtaatt tacaaagata
     1861 tccctgagga atatttagat caagcaaacg aatatcatgc taaaatgtta gatgctgctg
     1921 catcttatga tgatgatcta atgatgaaag ttttagaaga agaagaacca actgaagaag
     1981 aagttaaagc tgctatccgt aaaggtgtat tggctgtaga attattccca gtattatgtg
     2041 gttctgctta taaagacaaa ggggttcaac caatgttaga tgcagttatt gatttcttac
     2101 ctgctcctac tgatattcct tcaattaagg ggattgatga agatggtaat gaaattgaaa
     2161 aacatgcttc agacgaagaa ccgtttgctg cattagcgtt caaaattatg gctgacccat
     2221 ttgtaggaag attaactttc ttccgtgtat attcaggaac tatgaattca ggttcttatg
     2281 tattgaacgc tacaaagaat aaaaaagaac gtgttggacg tatccttcag atgcatgcta
     2341 ac
//
LOCUS       DAOMTA010000492         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2126_length_2341_cov_1.986877, whole genome shotgun sequence.
ACCESSION   DAOMTA010000492 DAOMTA010000000
VERSION     DAOMTA010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2126_length_2341_cov_1.986877"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..726)
                     /locus_tag="PLK68_11925"
     CDS             complement(97..726)
                     /locus_tag="PLK68_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRM91155.1"
                     /translation="MSQNNQAGQCLICFNDLNKSPSLYHLYYHATLCFHCLNQFSIYN
                     RTHDYHGYKLTILYYYNDFFKQLLFQYKGQGDYALKDAFLNAYPHFKTKYRRHLIALV
                     PSSQQDDLRRGFNPNEMIVRSFSNHIFTGLYKNSAYKQTSQTDRSQVSQIIKIKDGQR
                     LYNQNVIIFDDVITSGNTIMTCAKVISSYQPKTISIIVMASNQLDKLFK"
     gene            complement(693..1796)
                     /locus_tag="PLK68_11930"
     CDS             complement(693..1796)
                     /locus_tag="PLK68_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM91156.1"
                     /translation="MICPRCKNQDSQYFYTFKNITYCRKCVKIGSTCFSPSRSPSIIK
                     TVDYQLDYELTPLQNNISRQLLQRYQQHLNTSLKAVCGAGKTEITYEVIKYALNQGQR
                     VCFTTPRKELVIELAKRLQSQFKNISITTVYGGHSELVDGQFIICTTHQLYRYPQYFD
                     LLILDELDAFPYVNNEVLIGLLQNSIKGNYIYMSATLTNQPDLLMTKRYHGYLLDVPK
                     CYLTSSLVMYLWAIKKIRDFVRKKKPVLVYVPTIQLTNTVARIFKLFKLKSHAISSNT
                     KDIQKFIERLRHHQLDVLVTTTILERGITIDNVQVIILYGNNRIYTTATLIQICGRVG
                     RKTAHPSGSISIFTPYKTRAIKECLKTIKQDNA"
     gene            2067..2264
                     /locus_tag="PLK68_11935"
     CDS             2067..2264
                     /locus_tag="PLK68_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRM91157.1"
                     /translation="MQGKVKWFNAEKGFGFIDRGEGKDVFVHYSQITQDGYKTLNEGE
                     LVEFELYQSDRGMQAKHVVKI"
BASE COUNT          798 a          322 c          420 g          801 t
ORIGIN      
        1 gccctttaag tagaaaagta tttacgttac actatattta atggttattt aaaacgatat
       61 tattagtggt aaaacacaag ttttagtgtg taatatttat ttaaataatt tatctaattg
      121 attacttgcc ataacgatta tactaattgt tttgggctga taggaactga taacttttgc
      181 acaagtcatg atagtattgc ctgaggtaat aacatcatca aaaattatca cattttgatt
      241 atataaccgc tgaccatctt taatcttgat aatctgactt acttgactgc gatcagtttg
      301 gctggtctgt ttataggcgg aatttttgta taaaccagta aaaatatgat tactaaaact
      361 tcttacaatc atttcatttg ggttaaaacc tcgtcttaga tcgtcttgtt ggctgctggg
      421 aacaagcgcg atgagatgac gccgatattt ggttttaaaa tgaggatatg cattcaaaaa
      481 agcatctttt aaagcataat caccttgtcc tttatattga aataacaatt gtttaaagaa
      541 atcgttatag taatataaaa tcgtaagttt gtaaccatga tagtcatgag ttcgattata
      601 gattgaaaat tggttaagac aatgaaaaca tagtgtagca tgataataaa gatgatataa
      661 gctaggtgat ttattgagat cattaaaaca gattaagcat tgtcctgctt gattgttttg
      721 agacactctt taattgccct ggtcttgtat ggagtaaaaa tactaatact tccactggga
      781 tgtgctgttt tacgacctac tcgtccacat atttgaatca atgtagcagt tgtatatatc
      841 cgattattgc cgtataaaat aattacttga acattatcga tggtgatgcc gcgctctaaa
      901 atagtcgttg taactaaaac atctaattga tgatgtctaa gacgttctat aaatttttga
      961 atatctttag tgttagagct gatagcatga cttttaagtt taaacagttt aaagatgcgg
     1021 gcaactgtat ttgttaattg tatggtggga acataaacaa gtacgggttt tttctttctg
     1081 acaaaatctc taattttttt aattgcccaa agatacatta ccaaagatga agttagatag
     1141 cacttgggaa catctagaag ataaccatga tatcgtttgg tcattaataa atctggttga
     1201 ttggttagag ttgcagacat ataaatatag ttgcctttaa tgctgttttg gagtagacca
     1261 attaagactt cgttatttac gtaagggaaa gcatccaatt cgtctaaaat taataagtca
     1321 aaatattggg gatagcgata taactgatga gtagtacaaa taataaattg cccatctacc
     1381 aattcactat gaccgccata aacagtagtt attgaaatat ttttgaattg actttgcaat
     1441 cgctttgcta attcaattac tagttctttt cgcggtgtag taaagcagac tcgctgcccc
     1501 tggtttagtg catatttaat gacctcataa gttatttctg ttttaccggc accacaaacc
     1561 gctttaaggg aagtgttgag atgctgctga taacgttgaa gtaattgtct ggaaatatta
     1621 ttttgtaatg gtgttaattc gtagtcgagc tgataatcaa ctgtcttaat aatagaaggt
     1681 gatcgtgaag gagaaaaaca agttgaaccg atcttaacgc acttacggca ataagtaata
     1741 tttttgaaag tataaaaata ttgtgaatct tgattcttac atcgtggaca aatcatctta
     1801 tatcacccac ttatattgta actcaagatg ctaataaaag ggtctcaact tgatgctaaa
     1861 tttgtttcaa gaaagtttta ttttttttaa atgaaaatag agcatatatc gactttgatt
     1921 accgaattta tacttgacgc gacaacagtt ctatcaaaat agaacaaaat tcttatattg
     1981 gcaaactttg tcgagtgaaa tgaattcatc tttgaaaaca ataataatat actaacattg
     2041 accatattat taaaggggtg aatgagatgc aaggaaaagt gaaatggttt aacgcagaaa
     2101 aaggttttgg attcattgat cgtggtgaag gcaaagatgt atttgttcat tatagtcaaa
     2161 taactcaaga tgggtataag actttaaatg aaggtgaact agtagaattt gagctttatc
     2221 aaagtgaccg tgggatgcaa gcaaaacatg ttgttaaaat ctaagacaag ggataaaacc
     2281 ttgtctttta atttggactt taaaataata tatttaaatg ttataatacg gtagactaaa
     2341 c
//
LOCUS       DAOMTA010000493         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2133_length_2335_cov_2.078947, whole genome shotgun sequence.
ACCESSION   DAOMTA010000493 DAOMTA010000000
VERSION     DAOMTA010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_2335_cov_2.078947"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>2335
                     /locus_tag="PLK68_11940"
     CDS             100..>2335
                     /locus_tag="PLK68_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM91158.1"
                     /translation="MGNLMKKLKKTKMVKQFGILSLVLAMLIGVMSNGTIINAVDGNA
                     DANITFNSRIVEKVNGQDVDVSQADSGDSFFLAIYYAVSASTDASKYTECTLSITLPN
                     DIEFDETTDISNTGFDSIEQRTSLGKNILSIKSKELAAGQSGTLYLKMHFKNMTTKNG
                     TTAKFVDMKMTGSMVNENGSYTINPVSVPDASVTAKASQEWTVGKNVIQQADGNNYTI
                     KSIDGKDYYSVDYKVTAAPGSNLTDLGNNYGRLNCTTFELVDTLPVAPAGREDGGAAN
                     ISIKVGDKVLEEGADKDYELVLNENGTVKAIKIYYISTYESNPDDTNYVPDGAAMLTT
                     YTINANYSKDAYRVLPNEKDDIPFILDNKVELKYLPLGENTPKYVSANAPVNVGWVDE
                     NAPTTNLDITKYVTVNGDNSIVGDNKFIFDQEKQDCYYQNTDSRIAFGLYTDEACQNA
                     ATDIDGNTVGNSKVIDKDGKVNFAKISEGTYYLKETVGNLPFIGDGVDSANGETIYKI
                     EVKNENNKAVVYLDGKEIENGKLEINNLTTAQGFGYVAFNKVGTSATNSQHGPLAGVK
                     FILTNKVTGDKYETVSNDKGLVLFEGIIAGDYTVEEDFSSAEYDKPDVYKWDVKVEAN
                     KVNYPSLMLQDHNTGVPYVVNSSNKGKLIINKVDALTETKKLNGAEFTVYGPFESKET
                     ANIAIESEDFSSVDSFTLNGNEESKALVKGFYVYQETKAPNHYKLDENYYVTELKTQT
                     LNEVT"
BASE COUNT          867 a          276 c          478 g          714 t
ORIGIN      
        1 gcattactaa tcgttataat gaaaagaaga aacacgatga gattatttta acaaatgatt
       61 tgaagaaaat cagataaata aaagggggag aattagagaa tgggtaattt aatgaaaaaa
      121 ctcaagaaaa ccaaaatggt taaacagttt ggaattttat cgttagtatt ggctatgtta
      181 attggggtga tgtcaaatgg aaccataatt aatgcggttg atggtaatgc tgatgcgaac
      241 attacattta actcacgaat cgtagaaaaa gtcaatggac aggatgttga tgtttcacaa
      301 gctgattcag gagattcatt ctttttggca atttattatg ctgtttcagc aagtacagat
      361 gcttccaagt atactgagtg tacgttgagt atcacgttgc caaacgatat tgagttcgat
      421 gaaacaacag atattagtaa tactggtttt gattcaattg aacaaagaac atcactaggg
      481 aaaaatattc tatcaattaa aagtaaagaa ttggcggctg gacaatcagg tacgttatat
      541 ttaaaaatgc attttaagaa tatgacaact aagaatggca caactgctaa gtttgttgat
      601 atgaaaatga caggttctat ggttaatgaa aatggaagtt atacaattaa cccagtctct
      661 gtacctgatg cttcagttac cgcaaaagct agtcaagaat ggactgtagg gaaaaatgtt
      721 attcagcaag cagatggtaa taattacacg attaaatcaa tagatgggaa agactattat
      781 agtgtcgatt acaaagtaac tgccgctcca ggtagtaatc ttacggattt aggtaataat
      841 tatggtcggc ttaactgtac gacatttgaa ttagtggata ctttaccggt tgctccagca
      901 ggacgtgaag atgggggagc tgctaatatc tctatcaaag tgggagataa agtactagaa
      961 gaaggtgctg ataaagatta tgaattagtt ttaaatgaaa atggtactgt aaaagcaatt
     1021 aaaatttatt atataagtac ttacgaaagc aacccggatg atactaatta tgtaccagat
     1081 ggtgctgcaa tgctaactac ttatacgatt aatgcgaatt atagcaaaga tgcttatcgt
     1141 gtcttaccaa atgaaaaaga tgatattccg tttattttgg ataataaggt tgagttgaaa
     1201 tatcttcctt tgggagaaaa tacgcctaag tatgtaagtg ctaatgctcc tgtaaatgtt
     1261 ggctgggtag atgaaaatgc gccaacaaca aatttggata ttaccaaata tgttactgtt
     1321 aatggtgaca acagtattgt tggtgataat aaatttattt ttgatcaaga aaaacaagat
     1381 tgttattatc aaaatactga ttcacgtata gcgtttggac tgtatactga tgaagcgtgt
     1441 caaaatgctg cgactgatat tgatggtaac acagttggta atagcaaagt tattgataag
     1501 gatggtaaag taaattttgc taaaatttct gaaggaacat attatttgaa agaaactgtt
     1561 ggcaatttac catttattgg agatggtgtt gatagtgcta atggtgaaac gatttataaa
     1621 attgaagtta aaaatgaaaa taataaagct gtagtttatt tggatggtaa ggaaattgaa
     1681 aatggaaaat tagaaataaa taatcttaca actgctcaag gatttggtta tgttgcattt
     1741 aataaagtgg gaacaagtgc aacgaatagc cagcatggac cgcttgcggg agtaaaattt
     1801 attttgacca ataaagtaac tggagataaa tatgaaactg tttcaaatga taaaggatta
     1861 gtcttatttg aaggaattat tgcaggtgat tatactgttg aggaagattt ttcaagtgca
     1921 gaatatgata aaccggatgt ttataaatgg gacgttaaag ttgaagcaaa taaagtaaac
     1981 tatccatctt taatgctaca agatcacaat actggggtgc catatgttgt aaatagttcg
     2041 aacaaaggta aattaattat taataaagtt gatgcattga cagaaacaaa aaaattaaat
     2101 ggtgctgaat ttactgttta tgggcctttt gaatctaaag aaaccgctaa tatagctatt
     2161 gagtctgaag atttcagtag tgtggacagt tttaccttaa atggtaatga ggaatcaaaa
     2221 gctttagtta aaggttttta tgtatatcaa gaaactaaag cacccaatca ttataaactt
     2281 gatgaaaatt attatgtgac agaattaaaa acgcaaacat taaatgaagt tacag
//
LOCUS       DAOMTA010000494         2328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2146_length_2328_cov_2.001320, whole genome shotgun sequence.
ACCESSION   DAOMTA010000494 DAOMTA010000000
VERSION     DAOMTA010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2146_length_2328_cov_2.001320"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..2056
                     /locus_tag="PLK68_11945"
     CDS             2..2056
                     /locus_tag="PLK68_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM91159.1"
                     /translation="MPIAVLPIAGLFLGIGSSLTNETTISSLHLEGVLGNGTFLHDFL
                     IILTKVGSGLFNNLPLIFAAAVALGMAKKAKEVAVLSSIIAFFVMHTTISGMLSLNGS
                     ILDNQIINPDVLDGTITAVCGIMSLEMGVFGGIIVGLGVSYIHNRFYQIELPRALSFF
                     EGERFIPIISTITFLFVGIVMFYVWPFFQNGIYELGKLISTSGYFGTFVYGVIKRLLV
                     PFGLHHVFYLPFWQTAIGGSMVVNGAVIYGGQNIFFAQLADPNIVHFSSEATKYFTGE
                     FIFMIFGLPGAALAMYQTAKPENKKAIGSLLFSAALTSILTGITEPIEFTFLFAAPLL
                     FGVQVILAGSAYMVTHIFNIAVGLTFSGGLFDFIVFGVLQGNSKTSWVLLIPIGIIYF
                     MLYYFSFKYLIKKFDLKTPGREIDNMKLSIFKNPKASHRKLLNKGIEIDKQSQLIVRG
                     LGGRDNFTDLDCCITRLRATVSDNQLVNEGLLKQSGAAAVVMQGNGVQIIYGPKASSI
                     KSKLDEYLINIPDELDDYQLEKETDHTNLELGNIVDGEVLPIEQAPDQIFSHKLLGDG
                     VMIKPFHGLIVAPCDGEITMIYPTKHAIGMKLDNGYELLIHFGTDTVNLNGTGFEILV
                     KLNQRVQKGDLIWNADLEYIKENALDESILLIFTNLSNDLKIEKEYGKIKSGSTIMKI
                     CN"
     gene            2073..>2328
                     /locus_tag="PLK68_11950"
     CDS             2073..>2328
                     /locus_tag="PLK68_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HRM91160.1"
                     /translation="MYKIIKVLNNNTILACNETEEVIIMYKGIGFSKKVDDYFEVPRN
                     AKKFLMQKGYKAKSGSNDIINYIDPVYLEIASEIIRLIIEK"
BASE COUNT          753 a          281 c          478 g          816 t
ORIGIN      
        1 gctgccgatt gcagtattgc caattgcggg tttatttctg ggaattggta gttcattgac
       61 taatgaaaca actatttctt cactacattt agagggagtt ttaggaaacg ggactttttt
      121 acatgacttt ttaataatct taactaaagt ggggagtggc ctttttaaca atcttccact
      181 tatttttgct gctgcagtag cgttgggaat ggcgaaaaag gcgaaagaag tagccgtttt
      241 atcttcaatt attgcctttt ttgttatgca tacaacaatt agcgggatgc tttcattaaa
      301 tggaagtatt ttagataatc aaataattaa tcctgatgtt ttagatggaa cgattacagc
      361 ggtttgtggg atcatgtcat tggaaatggg ggtatttggt ggaataatcg tcggtcttgg
      421 cgttagttat atacataatc gcttttatca aattgaatta ccaagagcgt tatctttttt
      481 tgaaggagaa cggtttattc cgataatttc aacaataact tttttgtttg tgggaatcgt
      541 aatgttttat gtttggccat tctttcaaaa tggtatatat gaattaggta aacttatctc
      601 aacctcaggc tattttggaa cctttgttta cggtgtaatt aaacggttac tggttccttt
      661 tggcttacat catgtatttt atttaccatt ttggcaaact gcaattggag gttcaatggt
      721 cgtcaatggg gcggtaattt atggtggtca gaatattttc ttcgcacagt tagctgatcc
      781 aaatattgtg cattttagta gcgaggctac aaaatatttt actggtgaat ttatttttat
      841 gatttttggt ttaccgggag cagcattggc aatgtatcag actgctaagc cggaaaataa
      901 aaaagcgatt ggaagtttgt tgttctcagc ggctttaaca agcatcttaa cgggaataac
      961 ggaaccaatc gaatttactt ttttgtttgc tgctccactg ttatttgggg tacaagttat
     1021 cttagcagga agtgcttata tggttactca tatttttaat attgccgtgg gattgacctt
     1081 ttctggaggt ttatttgact ttattgtttt tggtgtattg caaggtaata gtaagactag
     1141 ctgggtattg cttatcccta ttggaataat ttatttcatg ctctattatt ttagttttaa
     1201 atatctaatt aaaaaatttg atttgaagac tccaggtaga gagatagata atatgaagtt
     1261 atctatcttt aagaacccta aagcaagtca tcgaaaattg ttaaataaag gaattgaaat
     1321 tgacaaacag tcacaattga ttgttcgagg gctaggtggt cgtgataatt ttactgactt
     1381 agattgttgt attaccagat tacgagcaac agttagtgat aatcaattag taaatgaagg
     1441 tttattgaaa caatcagggg cagcggcagt tgttatgcaa ggaaatggag ttcagataat
     1501 ttatggtcct aaagcatcat cgatcaaatc aaagctggac gagtatttaa taaatattcc
     1561 tgatgaatta gatgattatc aactggaaaa agagactgat catactaatc ttgaattagg
     1621 caatattgtt gatggtgagg ttttgccaat tgaacaggct ccagatcaaa ttttttcaca
     1681 taagctgtta ggtgatgggg ttatgatcaa gccatttcac ggtttgatcg ttgccccttg
     1741 tgatggtgag ataacaatga tttatccaac aaaacatgca attggaatga aattagataa
     1801 cggatatgaa ttgttgattc attttggaac tgatacagtt aatttaaatg gaactggttt
     1861 tgaaatatta gttaaattaa atcaaagagt gcaaaaaggt gatttaattt ggaatgcaga
     1921 tttagagtat attaaagaaa atgctttaga tgaaagtatt ttgttaatat ttacaaatct
     1981 atcaaatgat ttaaagattg aaaaagaata tggtaaaata aagagcggga gtacaattat
     2041 gaagatttgc aactaggaga gggaaaataa atatgtataa gatcattaaa gtattaaata
     2101 ataatactat tttagcttgt aatgagactg aagaagttat tattatgtat aaaggaattg
     2161 gattttcaaa gaaagtggat gattattttg aagtaccacg gaatgctaaa aaatttctta
     2221 tgcaaaaggg atataaagct aaaagtggtt caaatgatat aatcaattat attgatccag
     2281 tttatttaga aatagcatca gagattatta gactgataat agagaaat
//
LOCUS       DAOMTA010000495         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2148_length_2327_cov_4.926056, whole genome shotgun sequence.
ACCESSION   DAOMTA010000495 DAOMTA010000000
VERSION     DAOMTA010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_2327_cov_4.926056"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..528)
                     /locus_tag="PLK68_11955"
     CDS             complement(25..528)
                     /locus_tag="PLK68_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HRM91161.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            complement(546..1049)
                     /locus_tag="PLK68_11960"
     CDS             complement(546..1049)
                     /locus_tag="PLK68_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HRM91162.1"
                     /translation="MQETRVLVETKGTTGEETISYWFELPIDVAEFEEKLGIGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(968..1264)
                     /locus_tag="PLK68_11965"
     CDS             complement(968..1264)
                     /locus_tag="PLK68_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HRM91163.1"
                     /translation="MNDIFKDMQVKVGCEYISDLPSYKRKVWQEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTLQDVMKQQKGREEQCRKQGCWWKRKEQLAKKQYHTGLNCR"
     gene            complement(1352..1948)
                     /locus_tag="PLK68_11970"
     CDS             complement(1352..1948)
                     /locus_tag="PLK68_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM91164.1"
                     /translation="MLSTIYALLCIIVPCSLYQLIICKRQNKKQENKAIHFAWVYIFL
                     LYIYLTFSVAGIGSIWDIGHYDTIIRLEEINLLPFQSEGIMTYVLNIIMFLPLGFLIP
                     LIWKRYRNPLKIFMIGFGFSLSIELCQLFNRRNTDIDDLMMNTLGAVIGYFIWKLTKR
                     LFKNINHKSISLSKLEPIVYLTLAVLGEFLLFNWRIFL"
     gene            complement(2092..2313)
                     /locus_tag="PLK68_11975"
     CDS             complement(2092..2313)
                     /locus_tag="PLK68_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91165.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMSIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
BASE COUNT          736 a          492 c          362 g          737 t
ORIGIN      
        1 atgcttgtcg ctgatcttcc agcgttaata tggtatctcg cacatacctc ggcttgtttc
       61 aataaagcaa ccgcccatat ccaaatctcg tccgtaggct tcatagtcaa tgtagttctg
      121 taagctgggt ggaatatccc caagtgcctg caattcgtct atatagtagt aggcaacgtc
      181 tgtcatggtt tcacagtcgg gataataata aatatcgtcc ttgtgttcca cgacttcttc
      241 cagtgtcccg tagtggctga taaattcgtc cagacactct acgatatagt cgggaagttc
      301 ctctatcatt tcatacatct cattgagttc ttcaatggaa acatactcgc caatcgcaat
      361 gggaaagttg tcggtatcat ggatagcgta ttcctcatac tgttcattca agccgatttt
      421 ttctttcaca tcttcctcgt caatggggaa cgtgaaccaa gcaccgacta aatagccctc
      481 attgtatttt ccaagattag caatataaac cgccatatca tcaatcataa gcaccacctc
      541 atttcctact ctggcagttc aaagataccg tgttctgtta ctaaaaattt cccgtgttcc
      601 tgcaagtgag aagcgtaggc ttcaaagtca aaatagtatt cttgacagtc ctcggataaa
      661 tgtttgaacg tcgggtcgtt catcagcttt tgccttgcaa catctatcat gcttttgcag
      721 tcgggataaa cggtaatcac gttcctgcaa atataaagtg cttctaaatt ctcaaacacc
      781 gtaagtagtg atacatattc ttcctgcata tcacttgaaa gctggcggta cataaaatct
      841 aattcgttga gctggtacac gcttgtatgt tcgtggactt catcagcata aggcaacacc
      901 ttttcgataa tacggtaatc cccactttct gcaccgatac ctaacttttc ttcaaactcg
      961 gcaacatcta tcggcagttc aaaccagtat gatattgttt cttcgccagt tgttcctttc
     1021 gtttccacca gcacccttgt ttcctgcatt gttcctcacg tcctttctgt tgtttcatca
     1081 catcttgtaa ggtctggtac gacataccaa acacatattt agaaaaatct tctaactgtc
     1141 tttcttcata atcggcaggg ttcagtcgtt tcatttcctg ccacacctta cgcttgtaag
     1201 agggcaggtc ggaaatatac tcacagccga cttttacctg catatcctta aaaatatcgt
     1261 tcatttcatc actccaatct gaaatttagg tacgaaaaaa gcagtcaatc ctaagactaa
     1321 ctgctttttg tgtaaatatt ttttgttagc actagagaaa aattctccag ttgaataata
     1381 aaaattctcc taaaactgca agagttaaat atacaatagg ttctagttta gaaagagaaa
     1441 tagatttatg gttaatattt ttaaataacc tttttgtcaa tttccaaata aaatatccaa
     1501 taactgcccc tagcgtattc atcattaaat catcaatatc tgtatttctc cgattgaata
     1561 actggcaaag ttcaatggat aatgaaaaac cgaagcctat cataaaaatt ttcaaaggat
     1621 ttctgtatcg tttccaaatt aaaggaatta aaaagcccag aggtaaaaac attataatat
     1681 ttaaaacata agtcattatc ccctcagact gaaacggtaa taaattgatt tcctctaatc
     1741 ttataatcgt atcataatgt ccaatatccc aaatactacc tatacctgca acagagaacg
     1801 tcaagtagat atataacaga aaaatatata cccatgcaaa atgaattgct ttgttttctt
     1861 gttttttgtt ttgacgcttg caaatgataa gctggtataa ggaacaagga actataatac
     1921 atagcaaagc ataaattgta gataacatag taatcgtcct ttcacagtaa ttttaaataa
     1981 aatgatacag taaaaatctt aaaaaacacg aaagaatgta ttttgttttt atgaaagatt
     2041 ttatgttatc atatcatttt ttgctctgca attaaagata atttcaatat tttatgcacc
     2101 gataatctta ttgaacagct ctaacagtac atctttcaca ccgccagcat tgaataccaa
     2161 gccgacagca attaaggcaa ctaccaagaa gccgataagt ttggaaaact cacgcttgaa
     2221 acctaagtag ataccgataa ccacgatcga cataagcacc aagctctgtg catttgataa
     2281 aaaccattga tacaaatttt gtccgaaatt catttaattg tcctcgc
//
LOCUS       DAOMTA010000496         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2151_length_2325_cov_2.529515, whole genome shotgun sequence.
ACCESSION   DAOMTA010000496 DAOMTA010000000
VERSION     DAOMTA010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_2325_cov_2.529515"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..744
                     /locus_tag="PLK68_11980"
     CDS             97..744
                     /locus_tag="PLK68_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HRM91166.1"
                     /translation="MSLEIIQTLNPEYTIKSITDEAFRTYGKVIDNNIDEAIEFCIDF
                     VQAAKQDNFYLPSVLEVEQLSSIIELSHRVYGYLEIIAGIVAGDNVELSGIEYHQGSE
                     TIIAVTDYILVVGHIWDMQDDTYNSSKCELFYVPKGTIVECYSATLHYTPIAVSKEGF
                     ITICLLLKGTGDILEKRKKILKKKNKWFIAHQDNLEKIASGDYPGLLGRKIIIDH"
     gene            924..>2325
                     /locus_tag="PLK68_11985"
     CDS             924..>2325
                     /locus_tag="PLK68_11985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HRM91167.1"
                     /translation="MMQKIQRFGGAMFTPVLLFAFSGIVIGVCTVFQSDVIMGSIAAS
                     DTTWFKVWYVVKEAAWTIFRQVNLLFVISLPIGLAKKQNARACMESFVLYCTFNYALA
                     ALLSNWGSFVGIDYAIEAGSGTGLASVASIKTLDTGMVGALIVAGVAVWLHNRYFDTK
                     LPEWLGVFRGSSFVAMIGFPVMIGLAVIFFFIWPQVQHGIAGVQTFIISSGALGVWVY
                     TFLERILIPTGLHHFIYMPFMYDSVAIEGGIKTAWVAAMPEIAKSTASLRTLFPAGGY
                     ALYGFSKMFAPLGISAAFYATAKENKKKEVLGLMIPVTLTAMFAGITEPIEFTFLFIA
                     PVMFAVHSVLAASLATAQYLIGISGDFGSGIISNAALNWIPLGSAHWQQYLLSVVIGL
                     VFSGIWFIVFKFIIEKFDFKTPGREDDDEEVKLVSKAEYKASKEDTSNDPTGNIEVKG
                     GDAGKAAAFLAALGGKD"
BASE COUNT          731 a          304 c          494 g          796 t
ORIGIN      
        1 ttgttgatgc ctggttaact ggtgagtatg aaggtgggcg tcatcaagtt agaattgata
       61 tgctcaaaga tattgaagtg aggcaatgta agtaagatgt cattagaaat aattcaaact
      121 ttaaatcctg aatatacaat caaaagtatt actgacgaag cttttagaac ttatggtaag
      181 gttattgata ataatattga tgaagcaatt gaattttgta tagactttgt tcaggcagct
      241 aaacaagata acttttatct tcctagcgtt ttagaagtag aacaattgtc ttcgattatt
      301 gagctgtcac atcgtgttta tggttattta gaaataattg caggtattgt cgccggtgat
      361 aatgttgaat taagtggaat tgaatatcat caaggaagtg aaacaattat tgcagtcact
      421 gattatattt tagtagttgg gcatatatgg gatatgcaag atgacacata taatagttct
      481 aaatgtgaac ttttctatgt tccgaaaggt acgatcgttg aatgttatag tgctacttta
      541 cattacactc ctatagcggt tagtaaagaa ggctttataa ctatttgttt attattaaaa
      601 ggaactggcg atattttaga aaagcgtaaa aagattttaa agaagaaaaa taaatggttt
      661 attgctcatc aggataacct agaaaagatt gcctcagggg attatccggg tttattaggc
      721 agaaaaatca taattgatca ttaagcaaga tttttataat cttgcttctt ttttatttgt
      781 gaaatttcac gttaaaatgt gataatgggc tattatctta acatcggtac aaacagcctt
      841 caaaatattg taagcgcttt taattattgt taagataggc atatagggac ctatacatta
      901 gaaaagtgag gagagtttag aatatgatgc aaaaaattca aagatttggt ggggcgatgt
      961 ttacaccagt cttgttattt gctttttcag gaattgtcat cggtgtttgt acagtattcc
     1021 aaagtgatgt catcatggga agtattgcag catcggacac tacttggttt aaagtatggt
     1081 atgttgtaaa agaagcagca tggacaatct ttagacaagt caacttatta tttgttattt
     1141 cgttaccaat tggtctagct aaaaaacaaa atgctagagc atgtatggaa tcatttgttt
     1201 tatattgtac ttttaattat gcattagcag cactattaag caattggggt tcatttgtag
     1261 gaattgatta cgcaattgaa gctggtagtg gtacaggtct tgctagtgta gcaagtatta
     1321 aaacgttaga tactggaatg gttggtgcat tgattgttgc tggtgttgca gtatggttgc
     1381 ataaccgtta tttcgataca aaactgcctg aatggttagg agtatttaga ggttcttcgt
     1441 ttgtggcgat gattggtttt cccgtaatga ttggattagc agttattttc ttctttattt
     1501 ggccacaagt tcaacatgga attgctggtg ttcaaacatt tattattagc agtggcgcct
     1561 taggggtatg ggtgtatact ttcttagaaa gaattctgat tccaacagga ttacaccatt
     1621 ttatttatat gccgtttatg tatgattcag ttgcaattga aggcggaatc aaaactgctt
     1681 gggtagcagc aatgccagaa atcgctaaat caacagcttc attaagaaca ttgttcccag
     1741 ctggtggtta tgcattatat ggtttctcta aaatgttcgc tccactaggt attagtgcgg
     1801 cattctatgc aacagcaaaa gaaaacaaga aaaaagaagt attaggatta atgattccgg
     1861 ttactttaac agcaatgttt gcagggatta cagaaccaat tgaatttaca ttcttgttta
     1921 ttgcgccggt aatgtttgca gtgcattctg ttttggcagc atcattagca acagcacaat
     1981 atttaattgg aattagtggt gactttggaa gtggaattat ttctaatgcg gccttgaact
     2041 ggattccact tggatcagca cattggcaac aatatttatt atcagttgta attggtctag
     2101 ttttctcagg aatttggttt attgtattta aatttatcat tgaaaaattt gattttaaaa
     2161 cacctggtcg tgaagacgat gatgaagaag ttaagttggt ttcaaaagct gaatataaag
     2221 cttctaaaga ggatacaagc aatgatccaa ctggaaatat tgaagttaaa ggtggcgatg
     2281 ctggtaaagc agcagctttc ttagcggcat taggtggtaa agata
//
LOCUS       DAOMTA010000497         2324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2153_length_2324_cov_1.928162, whole genome shotgun sequence.
ACCESSION   DAOMTA010000497 DAOMTA010000000
VERSION     DAOMTA010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_2324_cov_1.928162"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..>347)
                     /locus_tag="PLK68_11990"
     CDS             complement(45..>347)
                     /locus_tag="PLK68_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HRM91168.1"
                     /translation="DSDLFFGNYHDERLYNNSPIQYLEGMSYDHPYVEMYRHCDIILC
                     CGQGAWEDEMIYSTHRMQELLENKDIPAWIDYWGYDVNHDWDWWQIQLPYFINHIL"
     assembly_gap    348..357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<358..935)
                     /locus_tag="PLK68_11995"
     CDS             complement(<358..935)
                     /locus_tag="PLK68_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRM91169.1"
                     /translation="MKVEYFKEYSNCLNREMEFKIYGHTGIPLLVFPAQDGRFYDFEN
                     FGMVSVIADKIEAGSIQVFCLDSIDGECWSDEGGNGPHRTYMMEQWYYYVMNELIPRI
                     FDINGTGQKIYTTGCSMGATHAANFMFRRPDIFQGCICLSGYYDSDLFFHDYVDDNGQ
                     FKYYNVGEKIYQSFLTIINSLEVDADVGYGLGA"
     gene            complement(935..2125)
                     /locus_tag="PLK68_12000"
     CDS             complement(935..2125)
                     /locus_tag="PLK68_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRM91170.1"
                     /translation="MNFLFISPMFPKNYWNFCDRMKNHGVNVLAIGDMPNESISEELK
                     NSVTEYCYVNNMENYDNVYRCVAYLASKYGKIDWLESNNEYWLELDAKLRTDFNVNTG
                     MKEDITKIFKTKSGMKAYYQKAGVKTARYHLVNTLENGRKFISEVGYPVVVKPDNGVG
                     AAATYKINNDQELEEFYNIDHITQYIMEEFVNGLILSYDGIANNEHQVLFETSHVFPN
                     SIMDVVNNHSNIHYYSLRKIPEELQKLGRSVVKEFPVNARFFHCEFFQLLEDRPGLGN
                     KGDFIGLEVNMRPPGGYTPDMMNWANDIDVYNIYADMVTYNHSEYYTNRSYHCIYCGR
                     RDGKKYYYNDDALMAMYGNHIVMNERMPDILSGAMGNYTYTARFETMDEVNDFIDKVL
                     KEVC"
BASE COUNT          807 a          475 c          257 g          775 t
ORIGIN      
        1 ggaaaatgcg gtgaaataaa tactacattc atatgtaatt ccccctataa aatatgattt
       61 ataaaatacg gcaattgaat ttgccaccag tcccaatcat gattaacatc atatccccaa
      121 taatcaatcc aagctggaat atccttattc tccaataatt cttgcatacg atgagttgaa
      181 tagatcattt catcttccca ggcaccttga ccacagcata gaataatatc acaatggcgg
      241 tacatttcta catatggatg atcatatgac attccttcta aatattgaat aggactatta
      301 ttataaagtc tctcatcatg ataattacca aaaaataaat cactatcnnn nnnnnnngca
      361 cccaaaccat aaccaacatc agcatctact tccaaactgt taattattgt taaaaatgat
      421 tgataaattt tttcccctac attgtagtat ttaaattgcc cattatcatc cacataatca
      481 tggaagaata aatcactatc ataatatcca ctcaaacaaa tgcatccttg aaatatatca
      541 ggtcgcctaa acataaaatt agctgcatga gtcgctccca ttgaacatcc tgtagtatat
      601 attttttgac cagtaccatt aatatcaaaa attcttggaa ttagttcatt cataacataa
      661 tagtaccact gctccatcat ataagtacga tgcggtccat ttccgccttc atcagaccaa
      721 cattccccat caatactatc taaacaaaat acttgtattg atcccgcttc gatcttgtct
      781 gcaatcacac ttaccatacc aaaattctca aaatcataaa accgaccatc ttgagcagga
      841 aaaactaata gcggaatgcc ggtatgccca tagattttaa attccatttc tctatttaaa
      901 caattactat attctttaaa atattcaact ttcattaaca gacctccttt aataccttat
      961 caataaaatc atttacttca tccattgttt caaagcgagc agtatatgta taatttccca
     1021 tcgctcccga taaaatatcc ggcattcttt cgttcattac aatatgatta ccatacattg
     1081 ccatcaaagc atcatcgtta taataatatt tcttgccatc acggcgtcca caatagatac
     1141 aatgatagga acgattagta taatattcac tatgattgta tgtgaccata tctgcataga
     1201 tattataaac atcaatatca ttagcccagt tcatcatatc tggcgtataa ccaccaggtg
     1261 gtcgcatatt aacttctaaa ccaataaaat cacctttatt tcctaagccg ggtcgatctt
     1321 ctaataattg aaagaactca cagtgaaaga atctagcgtt cactggaaac tcctttacta
     1381 cactacgccc taatttttgt aattcctcag gaatttttcg caaagaataa tagtgaatat
     1441 tactatgatt attaactaca tccataattg aattaggaaa aacatgtgat gtttcaaata
     1501 atacctgatg ctcattatta gcaattccat catatgaaag aatcaaacca ttaacaaatt
     1561 cttccataat atattgggta atatgatcaa tattatagaa ctcctccaac tcttgatcat
     1621 tattaatttt atatgtcgca gctgctccga cgccattgtc aggtttaaca acaacgggat
     1681 aacccacttc acttataaac ttcctaccat tttctaaagt attgacaaga tgatagcgag
     1741 ctgtttttac cccagctttt tgataataag ctttcattcc tgacttagtt ttaaaaatct
     1801 tggtaatatc ttctttcatc cccgtattga cattaaaatc tgttcttaat ttggcgtcga
     1861 gttctaacca atattcatta tttgactcaa gccagtcaat ttttccatat ttacttgcta
     1921 aatatgctac acaacgatat acattatcgt aattttccat gttattaaca taacagtatt
     1981 cagtaactga atttttaagt tcttcactaa tactttcgtt tggcatgtcc ccaattgcca
     2041 aaacattaac accatggttt ttcattcgat cacaaaagtt ccaataattc ttgggaaaca
     2101 ttggtgatat aaataaaaaa ttcatcaaac ccctccttta ttgtatacaa tgacttttca
     2161 ttgcttaaca taattataac atacaagtta aaaacatgtt taaaatatat taaatcaatc
     2221 tttataaaca aaaaaatcat caattattat aatcgatgat taatttattt aactggtttt
     2281 aaagaattcg cattagcttc aataactgca taattatgtc ttat
//
LOCUS       DAOMTA010000498         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2162_length_2321_cov_2.209620, whole genome shotgun sequence.
ACCESSION   DAOMTA010000498 DAOMTA010000000
VERSION     DAOMTA010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2162_length_2321_cov_2.209620"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="tmk"
                     /locus_tag="PLK68_12005"
     CDS             complement(<1..508)
                     /gene="tmk"
                     /locus_tag="PLK68_12005"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRM91171.1"
                     /translation="MKGKFITLEGPDGSGKTTVSKIVVEQLQMEGYKVLLTREPGGID
                     IAEQIRKIILDTNNITMDARTEALLYAAARRQHLVEKVAPALNDGYIVICDRFVDSSL
                     VYQGVGRKIGIEEVYQINQFAIGNIKPDATIFFDLPYEVGLARINNGERVADRLDLES
                     DDFHKDVYN"
     gene            714..1130
                     /locus_tag="PLK68_12010"
     CDS             714..1130
                     /locus_tag="PLK68_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRM91172.1"
                     /translation="MKLLPGFATFDDVFENMFSDPFFKGTSTHMRTDVKEVDDNYVLD
                     MELPGFDKKDISIELKDGYLNITANKSTDGKETDNQGNIIRQERFTGTCSRSFYIGDE
                     VKQEDIRASYDNGELKITLPKKAPKEITTNTYIPIE"
     gene            complement(1152..1313)
                     /locus_tag="PLK68_12015"
     CDS             complement(1152..1313)
                     /locus_tag="PLK68_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91173.1"
                     /translation="MRRVLFVITRAFVILLILNILTNNYFSYNFINIFVLSMLSLPGI
                     IVIYIISLL"
     gene            complement(1346..1939)
                     /gene="recR"
                     /locus_tag="PLK68_12020"
     CDS             complement(1346..1939)
                     /gene="recR"
                     /locus_tag="PLK68_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRM91174.1"
                     /translation="MNYPQAFQDLVDCFKRLPGIGGKSAERLAYHVLSMDKKYVDEFS
                     AAIGSIQTKIHYCKKCGHICEGEICDICQDPDRDQTTICIVEDPKDVFAMEKVKEYHG
                     LYHVLHGAMSIMDGKTMDDLNIASLFERLDDSIKEVIIATNPTRDGETTALYLAKLLS
                     KKNINTSRIANGLPIGSNIDYADELTLLKSLEGRKKI"
     gene            complement(1952..>2321)
                     /locus_tag="PLK68_12025"
     CDS             complement(1952..>2321)
                     /locus_tag="PLK68_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRM91175.1"
                     /translation="NTAKFASMLCDGKPVAAAPGGIIVAFEHQPNVNEVNETQNYYQL
                     KNFLKEVLGENYDFIAIKNSLWPDMRSKYIDMNRAGTLPAPEPIVLHHIGEFKEKRAE
                     LNDAQAMAVELFGDLVEFEE"
BASE COUNT          798 a          424 c          303 g          796 t
ORIGIN      
        1 cattataaac atctttatga aaatcatcag attctaaatc taagcgatct gccactcgct
       61 caccgttatt gatccgagct agtcctacct catatggcaa gtcaaagaaa atagtagcat
      121 caggtttaat attaccaatt gcaaactgat ttatctgata aacttcctct attcctattt
      181 ttctccctac accttgatag accaatgaac tatcaacaaa gcgatcacaa attacaatat
      241 acccatcgtt caaagccgga gctacttttt caactaagtg ttgacgtcga gctgctgcat
      301 ataataatgc ctctgttcgt gcgtccattg taatattatt agtatctaaa ataatcttcc
      361 ttatttgttc agctatatca attccaccag gctctctagt taataatact ttatatcctt
      421 ccatttggag ctgttcaaca actatttttg acacagttgt ttttccacta ccatctggtc
      481 cttctaacgt aatgaattta cccttcattt tatcttcctt tcaatcttta aatcaaaaac
      541 aattcacacc aaattatacc atatgtatga ttttgttaca atttaatagt ttttttagca
      601 ctctatactt gacagtgcta aaaaatatat tataatataa gtgtaatcca aaggaagtat
      661 cgattacatg aagtaatttc aattttgtat tattaacaaa ggagatggtt tatatgaaat
      721 tactacctgg ttttgctact tttgatgatg tttttgaaaa tatgtttagt gatccttttt
      781 tcaaaggaac aagtactcat atgcgaacag atgtaaaaga agtagacgat aattatgtac
      841 ttgatatgga gttacctggt tttgataaaa aagatatctc catcgaacta aaagatggtt
      901 atttaaatat tacagctaat aaatctactg atggaaagga aactgataat caaggaaata
      961 tcattcgtca agaacgtttt actggtactt gttcaagaag tttctacatt ggggatgaag
     1021 ttaaacaaga agatatccga gctagttatg ataatggtga gttaaagatt acattaccta
     1081 aaaaagctcc aaaagaaatt acaacaaaca catatattcc tattgaataa aaaatccggt
     1141 tcccggattt tttataataa tgagatgata taaattacaa taattcctgg taaggataac
     1201 attgagagta caaatatatt aataaagtta tagctaaaat aattatttgt taaaatattt
     1261 aaaatcaata aaattacaaa agctcgagta attacaaata aaactctacg cataaaaaga
     1321 cacctcatat aaatatatga agcgtttata ttttctttct tccttctaac gattttaaca
     1381 atgttaattc atcagcatag tcaatattac ttcctattgg taatccgtta gcgattctac
     1441 tagtattaat attctttttt gataataatt tagctaaata taaagctgta gtttctccat
     1501 cacgagttgg attggtcgca ataattactt ctttaatcga gtcgtctaaa cgctcaaata
     1561 aacttgcaat atttaaatca tccattgttt taccatccat tattgacata gcgccatgta
     1621 agacatgata taagccatga tattctttta ctttttccat cgcaaaaaca tccttaggat
     1681 cctcaacaat acaaatagtc gtctgatcac ggtctggatc ttgacaaata tcacaaattt
     1741 caccttcaca tatatgacca cattttttgc aataatgaat tttggtttga atactgccaa
     1801 ttgctgcaga aaattcatct acatattttt tatccattga taaaacatga tatgctaatc
     1861 gttctgcact ttttccacca atacctggca aacgcttaaa acaatcaact aaatcttgga
     1921 atgcttgggg ataattcatc gtaacctcct tttattcttc aaactcaacc aaatcaccaa
     1981 ataactcaac cgccatagct tgagcatcat ttagttcagc cctcttttct ttaaattcac
     2041 caatatgatg taaaacaatt ggctcaggtg caggtaatgt tcccgcccga ttcatatcaa
     2101 tatactttga gcgcatatct ggccaaagtg aatttttaat tgcaataaaa tcataatttt
     2161 caccaagaac ctcttttaaa aaattcttta actgataata attttgagtt tcattcacct
     2221 cattaacatt tggttgatgc tcaaaagcta caattattcc tcctggggca gcagcaactg
     2281 gcttaccatc acataacatt gaggcaaatt tagcagtatt t
//
LOCUS       DAOMTA010000499         2321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2163_length_2321_cov_1.920565, whole genome shotgun sequence.
ACCESSION   DAOMTA010000499 DAOMTA010000000
VERSION     DAOMTA010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2163_length_2321_cov_1.920565"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PLK68_12030"
     CDS             complement(<1..175)
                     /locus_tag="PLK68_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM91176.1"
                     /translation="MKGIVLAGGSGTRLYPLTQVTSKQLLPIYDKPMIYYPMSVLMNA
                     GIRDILIISTPADT"
     gene            371..1225
                     /locus_tag="PLK68_12035"
     CDS             371..1225
                     /locus_tag="PLK68_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM91177.1"
                     /translation="MKDIRNIIGILLGNTIYALGVTMFILPNNLITGGTTGMALLVNA
                     TTGFSITVFVSIFNVSMFLIGWKVLGKKFALTTLISSFYYPFILGILQGVFKNEMMSK
                     DTLLCVILAGLMIGTAIGLVIRCGASTGGMDIPPLILNKKLGIPISVSMYGFDFIILL
                     GQMVIRNREMVFYGILLVLIYTVVLDKVLVIGKSQMQVKIVSAKFNEINEKIISVLDR
                     GTTLIHSETGFKHNQYPVVLTVVNNRELTQLNNYVYDIDPDAFMIINKVNEVRGKGFS
                     SAKKYENK"
     gene            complement(1296..2024)
                     /locus_tag="PLK68_12040"
     CDS             complement(1296..2024)
                     /locus_tag="PLK68_12040"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRM91178.1"
                     /translation="MKLIIEDSKEKMSESAMQILLGTMMQDKRVNISLTAGRSPELLY
                     KMMIPYVKDQAKFADVQYYLFDEAPYIGKTAKEDGENWKEMQKVFFEPANIPADRVHI
                     TTMDNWETFDEQIRNAGEIDAMLIGLGFDGHFCSNCPRCTPMDSYTYALERKIKNAVN
                     PAYADKPQQPVTLTMGPKSLMRVKHLVMIVTGKEKAEILKQMLDSPITDELPATILKL
                     HPNFTVICDQDAASLLDLNNYKRL"
BASE COUNT          763 a          316 c          408 g          834 t
ORIGIN      
        1 gtgtatctgc aggtgtcgaa ataatcagaa tatctctgat ccccgcattc ataagcactg
       61 acattggata ataaatcatt ggtttgtcat aaattggtaa taattgttta cttgttactt
      121 gagttagagg atataatctt gttccagatc ctccagctaa tacaatacct ttcatattaa
      181 aaagcctcct ttatttcatc ataatcttaa accattacgt aacgtaatgg tcaagtgtaa
      241 ttgtataatg atattaaagt ttttagtact aaaaagatgt ctaaaagaat taattagtaa
      301 ttgtattgta taaaaaacaa ttatgattta aaatagataa agttgaaagt aatgatgaaa
      361 gagtgagaaa atgaaagata tcagaaatat aatcggaata ttattaggta atacaattta
      421 tgctttagga gtaacgatgt ttattttacc taataattta attacaggtg gaacaacagg
      481 gatggcgttg ttagtaaatg cgacgactgg attttcaata actgtctttg tttcaatttt
      541 taatgtcagt atgtttttaa ttgggtggaa agtattaggg aaaaagtttg ctttaacaac
      601 tttgatcagt tctttttact atccattcat tttagggatt cttcaaggtg tttttaaaaa
      661 tgaaatgatg agtaaagata cattattatg cgttatttta gcgggattaa tgattggtac
      721 agcaatcggt ttagtaattc gttgtggagc tagtactgga ggaatggata taccgccatt
      781 gatcctaaat aaaaaattag gtattcctat ttcagtaagt atgtatggtt ttgattttat
      841 tattttacta ggacaaatgg tcattcgtaa tcgagaaatg gttttttatg gaatcttgct
      901 agtgctgatc tatactgttg tcttagataa agtattggtt attggaaaat cacagatgca
      961 agtcaaaata gttagcgcta aatttaatga gataaatgaa aagattatta gtgttcttga
     1021 tcggggaact acactgattc atagtgagac gggttttaag cataatcagt acccagtagt
     1081 tttaacagtt gtaaataatc gagagttgac ccagttaaat aattatgttt atgacatcga
     1141 tcctgatgcc tttatgatta tcaataaggt aaatgaggtt aggggaaaag gcttctcaag
     1201 tgcaaaaaaa tatgaaaata aataaaacgg ctaatatgca gcgctcgcct aaagtcagtc
     1261 taactttagg cgggacacta caataggccg ttttgttata atcttttata gttatttaaa
     1321 tctagtaatg aagcagcatc ttgatcacaa ataaccgtaa agttaggatg cagttttaaa
     1381 atggtagctg gtaattcgtc agtgattggt gaatcaagca tttgctttaa aatttctgct
     1441 ttttctttac ctgtgacaat cattactagg tgttttactc gcatcaaact ctttggaccc
     1501 attgttaaag ttaccggttg ttggggttta tcagcataag cgggattaac cgcattttta
     1561 attttgcgtt ctaatgcata agtatagctg tccattggtg tacagcgagg acagttacta
     1621 caaaagtgac catcaaatcc taacccaatc agcattgcat cgatttctcc ggcattacga
     1681 atctgttcat caaaggtttc ccaattgtcc atagtagtga tatgaactcg atcggcagga
     1741 atattagctg gttcaaaaaa cactttttgc atttctttcc agttttcacc atcttcttta
     1801 gctgtttttc caatatatgg ggcttcatcg aataaataat attgcacatc agcgaatttt
     1861 gcttgatctt taacatatgg aatcatcatt ttatataata attcgggtga acgaccagcg
     1921 gttagtgaaa tatttacacg cttatcttgc atcattgttc cgagtaaaat ttgcatcgca
     1981 ctttcactca ttttttcttt tgaatcttca ataattaatt tcataatctt tctccttgat
     2041 tttcatattt tatgaaacat cttggataaa gaagtaaagc ttacacaaag gctattctaa
     2101 ggcaataaag atattaggtc aaggtaattt tggtaaaata attaataata tttatatttt
     2161 ttaaaataac tttttttagc tattttttca ttttatcata aattcaacta aaataattag
     2221 tgatttccaa atattggaaa ttatttcgca attatgcaaa ataattgttt atagactggt
     2281 tgagtttata ttatataatc atggggaggt taaaagaagg g
//
LOCUS       DAOMTA010000500         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2164_length_2320_cov_2.147020, whole genome shotgun sequence.
ACCESSION   DAOMTA010000500 DAOMTA010000000
VERSION     DAOMTA010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_2320_cov_2.147020"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..895)
                     /locus_tag="PLK68_12045"
     CDS             complement(107..895)
                     /locus_tag="PLK68_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM91179.1"
                     /translation="MKYQPLESKSALNKVSGRFPFKWDLNIYRGCQHGCIYCYAIYSH
                     KYLDNNDYFGTIYYKENILSCLEKELSSPKWKHELVNIGGVCDSYQPLEEQLQIMPEI
                     LKLMIKYKTPIIISTKSSLILRDIELINELSKITYVNIACTIITVDDDLRKIIEPGSS
                     PIIERFKVIDQIKKETKAHAGIHIMPIIPYLTDQPGNLNGLYKMAKKVNADYVLPGTL
                     YLRGQTKPYFLNCIKKYNYDLYQKIASLYYQKNALKTYKPMILK"
     gene            1027..1893
                     /locus_tag="PLK68_12050"
     CDS             1027..1893
                     /locus_tag="PLK68_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91180.1"
                     /translation="MKTKYVLVLMAMMFVISGCREEVTKEVGDTTINIAELNGKREID
                     KNDKIFANNNQGKHDLLMLMANSVDHYNLISGTMLSTLSYQGQKSESKYIFQVNVPEY
                     QSYIYLVPSKNEESQVTFRDSERMYFFVGDLVSLNNNLDELINKQMMTVEDVSKDDSL
                     FKYKDLTLEERVENMDISRVANDLFGDVAPYLLPEDIALRQIGTAYNNYEIKGEEKYL
                     NRDTYRVEGKINDSLYNSDSTFSMLVDKQTGIVLKYTRTTKDSLVEMKMEGISIDEMN
                     EENMYAKYLSQK"
     gene            1996..2071
                     /locus_tag="PLK68_12055"
     tRNA            1996..2071
                     /locus_tag="PLK68_12055"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2029..2031,aa:Asn,seq:gtt)
     gene            2073..2148
                     /locus_tag="PLK68_12060"
     tRNA            2073..2148
                     /locus_tag="PLK68_12060"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2106..2108,aa:Glu,seq:ttc)
     gene            2153..2228
                     /locus_tag="PLK68_12065"
     tRNA            2153..2228
                     /locus_tag="PLK68_12065"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2186..2188,aa:Val,seq:tac)
     gene            2244..2319
                     /locus_tag="PLK68_12070"
     tRNA            2244..2319
                     /locus_tag="PLK68_12070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2277..2279,aa:Thr,seq:tgt)
BASE COUNT          800 a          299 c          443 g          778 t
ORIGIN      
        1 gacccgtttt tgatggtcga tatgcacaaa gctgttgccc gtctgtcaga ggcaatgaaa
       61 cttaatcaga aaattctgat ttatggcgat tacgatgtgg atggtactac ttcagtatca
      121 ttggtttata agtttttaaa gcattttttt gataatatag agaagcaatt ttttgatata
      181 aatcataatt atattttttt atacaattaa ggaaataggg tttagtttgc cctcgtaaat
      241 agagagtacc gggtaacaca taatcagcat ttactttttt tgccatttta tataatccat
      301 ttaaatttcc gggttgatca gtgagataag ggataattgg cataatgtga ataccagcat
      361 gagctttagt ttcctttttg atttggtcaa ttactttaaa tcgttcaata ataggtgaac
      421 ttcctggttc gatgatttta cgtaagtcat catcaacagt aataatagta caagcaatat
      481 taacataggt tatttttgat aattcattga ttagttcaat atcacgaagg attaatgatg
      541 atttggtaga aataataata ggtgttttat acttaatcat taattttaaa atttctggca
      601 ttatttgtaa ttgttcttct aaaggctgat aactatcaca aacaccccca atattaacta
      661 attcgtgttt ccatttaggt gatgataatt ctttttctaa acaagataaa atattctctt
      721 tataatagat cgtgccaaaa taatcattgt tgtcaaggta tttatgactg taaatagcat
      781 aacaataaat acaaccatgt tgacagccgc gatagatatt taaatcccac ttaaagggaa
      841 atcgcccact aactttattt aaggcgcttt tagattctaa tggttgatat ttcattttat
      901 caccttctga tttaatcata acatattgct tgaaatatgt atgtaaagaa tcgttgttgg
      961 gaatattaat tctacataat tctaataatt gttatgatta aataaaaata aaaaggatga
     1021 atggtgatga agactaaata tgtgctagta ttgatggcaa tgatgtttgt tattagtggc
     1081 tgtagggaag aggtaacaaa ggaagtagga gatacaacga ttaatattgc agaactaaat
     1141 ggcaaaagag aaattgataa aaacgataaa atatttgcga ataataatca ggggaagcat
     1201 gatttattaa tgttgatggc taatagtgtg gatcattata atttaattag tggaacaatg
     1261 ctttcaacgt taagctatca aggacaaaaa agtgaaagca agtatatttt tcaagttaat
     1321 gttccagaat atcagtcata tatttattta gttccttcaa aaaatgaaga aagccaagta
     1381 acttttagag atagtgaaag aatgtatttt tttgttgggg atttagtgag tctaaataat
     1441 aacttagatg aattaatcaa taagcaaatg atgacagttg aggatgtaag taaagatgat
     1501 agcttattta aatataaaga tttgacactt gaggaaaggg ttgaaaatat ggatattagt
     1561 cgagtagcta atgatttgtt tggcgatgta gcaccatatt tattacctga agatattgca
     1621 ctcagacaaa taggtactgc ttataataat tatgaaataa agggagaaga aaaatatctt
     1681 aatcgagata cttatagagt agaggggaaa ataaatgata gtctctataa tagtgatagc
     1741 actttttcta tgttggtcga taaacaaact ggaattgttc ttaaatatac taggactaca
     1801 aaagatagtc tggtagagat gaagatggaa ggtatttcga ttgatgaaat gaatgaagag
     1861 aatatgtatg ctaaatatct tagtcaaaag tagaattttt actacttttt tcttttttga
     1921 taaaaaaata aatttttttc ataaaaaggg ttgcatcatt ctagggataa tgatattata
     1981 ttagggcact aagttgcttg aatagctcag ttggtagagc aatcggctgt taaccgatcg
     2041 gtcgtaggtt cgagtcctac ttcaagcgcc atggcctgtt ggagaaacgg ttaactcaca
     2101 tgcctttcac gcatgcattc acgggttcga atcccgtaca ggtcaccatt atggaggttt
     2161 agctcagttg ggagagcatc tgccttacaa gcagagggtc agcggttcga gcccgttaac
     2221 ctccaccatt taaaataaaa taggccggct tagctcaatt ggtagagcaa ctgacttgta
     2281 atcagtaggt tgagggttca agtcctttag ccggcaccat
//
LOCUS       DAOMTA010000501         2319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2167_length_2319_cov_2.209806, whole genome shotgun sequence.
ACCESSION   DAOMTA010000501 DAOMTA010000000
VERSION     DAOMTA010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2167_length_2319_cov_2.209806"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..879)
                     /locus_tag="PLK68_12075"
     CDS             complement(250..879)
                     /locus_tag="PLK68_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91181.1"
                     /translation="MGCCRRNFNTDEFKAWLSTLHEEQPGTEFCIKEDDQKETYNYLK
                     DNLFGQIVVWNNSVVEEIITDCTNNEQIFYLHYEMEDRNQIKELIKSFKRRMFCVCQK
                     EYEVSEDDGERNILLCCSSGLTTAMFAQMLQDFSNNNHLPYHFSATSIYDDDTLSKEY
                     DLVLMAPQVQHYTKSMAAKYHKRFLIMNPVDFAQYNCNNIVSRVQEVFL"
     gene            complement(954..>2319)
                     /locus_tag="PLK68_12080"
     CDS             complement(954..>2319)
                     /locus_tag="PLK68_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91182.1"
                     /translation="IMLIIPLNFMNVFNFRAMLQLLFFLSLGGAFFNTGMFDPSKDKY
                     YAVILMKMDGREFVLSQYLYTLFRHYVGYMTAFMILSFTLQYPFWLSYILPLFIIGLK
                     LMAVSAYLKDFKRNRKVRSENNNSPAIIGFSIGCLLVGYALIFSNLIIPIKMMLIVLI
                     ILSILAVILMSYVLKYDDYHQLSHKLLKNIDSLLNTDTQDIINDSYHKMITLEADTKS
                     NKTGFEYFNELFMQRHKKILWKTAKRQTYMIAFIIVVIIGILFYDVETQKDANNAMTS
                     ILPFFVFIMYLLNTAKNITQAMFVNCDHSMLTYSFYRIPKNILILFKIRLREIVKINL
                     LPAVVLALGYMAILFICGGIDQAFLAFISFISIISMSIFFSTHYLILYYLLQPYNAHT
                     EVKSPIYSTIISLTYIICYAFTKLELPILSFGIVCILFCIGYCVIACILVLKWAGNTF
                     KIRN"
BASE COUNT          905 a          366 c          250 g          798 t
ORIGIN      
        1 agtggttgaa aatgccgatt atcaaacatt aaataatcca ttacctatat tatataataa
       61 ttaagttaaa aattaaatta atacatcttt tctaactaat ttttttgcct ttatagccta
      121 tcttcttaac catgccatca ttgctcaatt gataatctaa acgattaata attaataaca
      181 aatacgactt taatattaaa gaaactcact ataatataca gtgagtcata aatattcaaa
      241 atttagtctt tataaaaata cttcctgcac acgagaaaca atattattac aattatattg
      301 tgcaaaatca acaggattca ttattaaaaa acgtttgtga tacttcgctg ccatactttt
      361 agtatagtgt tgaacttgtg gtgccatcaa cactaaatca tattctttcg atagcgtatc
      421 atcatcatag atactagtgg ctgaaaaatg gtatggtaaa tgattattat tactaaaatc
      481 ttgtaacatt tgagcaaaca ttgctgttgt taacccacta gaacaacaca gtaaaatatt
      541 tcgttcacca tcatcttcac tcacttcata ctctttctgg caaacacaaa acattcttcg
      601 cttaaaactt ttgattaatt ctttaatctg attacgatct tccatctcat aatgaagata
      661 aaaaatttgt tcattatttg tacaatcagt gatgatttct tctacaaccg aattattcca
      721 tacgacaatt tgtccaaaaa gattatcttt aagataatta tatgtctctt tttgatcatc
      781 ctctttgata caaaattcag ttcccggctg ttcttcgtgt aaagtggata gccatgcttt
      841 aaattcatca gtattaaaat ttcttctaca acatcccatt tttagccctc ctatctactt
      901 atatctataa ataaataata tcatcatcaa actaaacttt caatttagtt aatttaattt
      961 cgaatcttaa aagtattccc agcccactta agtactaaaa tacaagcaat gacacaatag
     1021 ccaatacaaa atagaataca aacaattcca aaggaaagaa ttggtaactc aagtttagta
     1081 aaagcataac aaataatata ggtcaatgaa ataattgttg aataaatagg actttttact
     1141 tcagtatgcg cattatatgg ctgcaataaa taatataaga ttaaatagtg tgttgaaaaa
     1201 aatatgctca ttgaaattat tgagataaaa gatataaatg ccagaaatgc ttgatcaatt
     1261 cctccacaaa taaataaaat agccatatat cctaatgcta aaacaactgc tggcaataaa
     1321 ttgattttaa ctatttctct taaacgaatt ttaaataata ttaaaatatt tttaggaatt
     1381 cgataaaaag aataagtcaa cattgaatga tcgcagttta caaacattgc ttgtgtaata
     1441 tttttagcag tatttaataa atacataata aaaacaaaaa aaggtaaaat acttgtcata
     1501 gcattatttg catctttttg agtttcaaca tcataaaata agataccaat tatcacaact
     1561 attataaatg caatcatata agtttgtcgt ttagctgttt tccataaaat tttcttatgt
     1621 ctttgcataa ataattcatt aaaatactca aaaccagttt tattactttt agtatcagcc
     1681 tctaaagtaa tcattttatg atatgaatca ttaataatat cttgagtatc tgtatttaat
     1741 aacgaatcga tattttttaa taatttatga ctaagctgat gataatcatc atactttaat
     1801 acataactca tcaaaataac cgctaatata ctaaggatta ttaaaacaat cagcatcatc
     1861 ttaatcggaa taatcagatt agaaaaaata agtgcataac ccactaataa acatccgata
     1921 ctaaatccaa ttattgctgg tgaattatta ttttcacttc gtacctttcg attcctttta
     1981 aaatctttaa gataagcact aacagccatt agctttaaac caataataaa taacggtaat
     2041 atataactta accaaaaagg gtattgtaat gtaaaagata aaatcatgaa tgctgtcata
     2101 tatccaacat aatgtcggaa cagagtatat aagtattgtg atagtacaaa ctctcgtcca
     2161 tccattttca ttaaaataac tgcataatat ttatctttcg agggatcaaa cattcctgta
     2221 ttaaaaaatg ctccgcctaa acttagaaaa aataataatt gtaacattgc acgaaagtta
     2281 aatacattca taaaattcaa aggtattatg agcataatt
//
LOCUS       DAOMTA010000502         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2176_length_2314_cov_2.248340, whole genome shotgun sequence.
ACCESSION   DAOMTA010000502 DAOMTA010000000
VERSION     DAOMTA010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2176_length_2314_cov_2.248340"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..895
                     /locus_tag="PLK68_12085"
     CDS             140..895
                     /locus_tag="PLK68_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM91183.1"
                     /translation="MDSTIKFDGLANLYEDGRPEYPTILIEKLYTDYGFKSTSIIADI
                     GAGTGKFSKLLLEKGSKVFCVEPNKDMRNELVNKLNKYNKLHAINGTAGNTTLDTSSV
                     DFIISAQAFHWFNVHEFKRECKRILKLEGRVALVWNVREMESPLNQECFQIYKQYCQN
                     FVGFNGGIKRDDERIKEFFDMHYKRMEFPNPLFFDQKSFIKRCISGSYSLKKTDQNYF
                     EYIAALENVFDKYSNNGQLVMENKTVVYIGRLK"
     gene            1415..2029
                     /locus_tag="PLK68_12090"
     CDS             1415..2029
                     /locus_tag="PLK68_12090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM91184.1"
                     /translation="MGFNDERKRMNEGELYRSNDAEILKEQLQCVEKLYDYNVTRPSE
                     KQKRKGLLKEMFAVIGDGCHIEPPFHANLGGKYVHFGKGVYANFNLTLVDDVEIYVGD
                     YTMMGPNVTIITGTHPILPELRKEAYQFNLPVYIGENVWIGAGTIILPGITIGDNSVI
                     GAGSIVTKDIPKNVVAYGQPCTIKREINDHDKIYYYKERKIKSV"
BASE COUNT          870 a          252 c          436 g          756 t
ORIGIN      
        1 aagaaaaact tattaaagat gacatggctt tatctggtgt tattacgaga ggttgtggtg
       61 gaatgcaaag tattgaagaa gcaacaacaa gattaataac tgataaaaaa agacttatca
      121 ataaaggagt attaataata tggatagtac aataaaattc gatggcctag caaatcttta
      181 tgaggatggt agaccggagt acccgactat attaatagaa aagttatata ctgactatgg
      241 ttttaaatca acatcgataa tcgcagatat aggtgctgga actggtaaat tttcaaagct
      301 gttattagaa aaaggtagta aggttttttg tgtcgaacct aataaagata tgagaaatga
      361 attagtcaat aaattaaata aatataataa attgcatgct atcaatggaa ctgctggtaa
      421 tactacgtta gatactagtt ctgttgattt tattatatct gctcaggcat ttcattggtt
      481 caatgttcat gagtttaaaa gagaatgtaa gagaatctta aaattagaag ggcgggtagc
      541 attggtttgg aatgtacgag aaatggagtc accattgaat caagagtgct ttcaaattta
      601 taaacagtat tgccaaaact ttgttggttt taatggcggg attaaacgcg atgatgaaag
      661 aataaaggaa ttttttgata tgcactataa gcgtatggag tttccaaatc ctttattttt
      721 tgaccagaag agttttatta agcgctgtat ctcaggttca tattctttaa aaaaaactga
      781 tcagaattat tttgaataca ttgctgcctt agaaaatgta tttgataaat atagcaataa
      841 cggacagtta gtaatggaga ataagacggt tgtttatata ggaagattaa aataaatgta
      901 gttcaatgtc aatgagaaat acttttatag ctttaccatt agttaaaggg gcatatatga
      961 ttatatagtc ataaaagcgc tttcttaatt taggcggtaa taggaaggaa gagatggcca
     1021 tgtaccatag attcccaact aaatatcttg aattagaatt gactgaaaca atggtctttg
     1081 cctaactaga agtttttagt aaaattattg agaaaattca ttaggctgga ttcaactact
     1141 ctatggatga ttttggaagt ggctattctt ttttaggaat ataaaaagat tcagatatcg
     1201 ttaagcttga tggtacattc ttttcttctg aaacgatgaa taaggggaaa gaaaatatta
     1261 ttgataagaa atagtgatta aatcactgaa caaattattt atagttaaca ttttgatagc
     1321 attatcaaac attagatact attacattga ttaaagctat attataatat aattggattt
     1381 aaaatttgag atataaatgt aagggagtta aaccatgggc tttaatgatg aaagaaaacg
     1441 aatgaatgag ggtgaacttt atcgttcaaa tgatgcggaa attctaaaag aacaactaca
     1501 gtgtgtagaa aaattatatg attataatgt aacaagacct tcagaaaaac aaaaacgtaa
     1561 gggattgcta aaagaaatgt ttgcagtgat tggtgatgga tgtcatattg aaccaccatt
     1621 tcatgctaat ttgggtggga agtatgtaca ttttggtaaa ggagtatatg ccaatttcaa
     1681 tctaacttta gtagatgatg ttgagattta tgttggtgat tatactatga tgggtccaaa
     1741 tgttacaatt attactggaa ctcacccaat tttaccggaa ttaagaaaag aagcatatca
     1801 attcaatctt ccagtttata ttggtgaaaa tgtatggatt ggtgctggaa caattatttt
     1861 accaggtatt acaatcggtg ataatagtgt tattggtgct ggaagtattg taacaaaaga
     1921 tattcctaaa aatgttgttg cttatgggca accatgcaca attaaacggg aaataaatga
     1981 tcatgataaa atttattatt ataaagaaag aaaaatcaaa agtgtttaat atttttgatt
     2041 tttttattta tggattaaat acgtattact aataatatta gtaatagctt taggtttaag
     2101 ctataatata aaagtataat gtaatattat tgtgatccaa tccaagaaga tagtaagatg
     2161 cttaaagaag caaagaatga ggatttagaa gcactaccag aactttatac acaatttgaa
     2221 aacgtggtta ctgcttaagc attggaaagt tagcccttgt tcaatgactc taaatcaata
     2281 tgcctaatcg aattaatatt aatgatagaa caaa
//
LOCUS       DAOMTA010000503         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2177_length_2313_cov_1.848096, whole genome shotgun sequence.
ACCESSION   DAOMTA010000503 DAOMTA010000000
VERSION     DAOMTA010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2177_length_2313_cov_1.848096"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /gene="rnc"
                     /locus_tag="PLK68_12095"
     CDS             complement(<1..475)
                     /gene="rnc"
                     /locus_tag="PLK68_12095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRM91185.1"
                     /translation="MKNHDYERLEFLGDAVLQYHVSRHIFDLYPELPEGRLTKLRAKL
                     VREESLARFARELDLGPLIYLGAGELNNGGRDRDSVLADIFEAFMGAICHDCGPEYVE
                     KMLDITIYKHVEDVNYDEITDFKTKLQELIQADQRKTVTYDLISSSGPSNNPVFEM"
     gene            complement(615..1589)
                     /gene="plsX"
                     /locus_tag="PLK68_12100"
     CDS             complement(615..1589)
                     /gene="plsX"
                     /locus_tag="PLK68_12100"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRM91186.1"
                     /translation="MKLGIDAMSGDLGSRIVVEACLSFLEKNKTDELYVVGKIEELEA
                     LKPYDQVTLIDAREVLEMTENILAIRRKKESSMVKTMMLARKGEVDAVLSCGNTGAYY
                     ASAMLFLKRIEGVEKSCLMAMMPTYSDNKVAMLDVGANSENTAEQLKSFAIMGNAYAK
                     NVLKITNPKIALLNIGSEHHKGDEIHQETYKLLEDMQEINFVGNIEGKEILDGEVDVV
                     VTDGFTGNVALKTIEGVAKVLVKSLKDGFMSSTRTKAGAVLAKPALKQLLTKFDTKAA
                     GGALMMGFVKPVIKAHGSSDAIAFENAINLAFEMVSSDVVEKMKEGLN"
     gene            complement(1627..>2313)
                     /locus_tag="PLK68_12105"
     CDS             complement(1627..>2313)
                     /locus_tag="PLK68_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRM91187.1"
                     /translation="KPILNRLKEYLASGGQCYVVCPLVEESEAIDSRDATGIYNGMKA
                     YFKEQYQIGLLHGKMDDETKDQIMAAFKANEIQILVSTTVIEVGVDVSNANWIVIYNA
                     ERFGLSQIHQLRGRVGRSDQQGYCFLLSNSSSQEALERLEFLRNCHDGFEVSYYDLKL
                     RGPGDILGNQQSGLPVFSVGNIFEDANILEISRKDALELLESKSNDLIYLKLIKEIEE
                     QLINNNKYID"
BASE COUNT          732 a          518 c          267 g          796 t
ORIGIN      
        1 ccatttcgaa tactggatta tttgacggtc ctgatgatga gattagatca taagttaccg
       61 tctttctctg atcagcctga atcaattcct gcaattttgt tttaaaatct gtaatttcat
      121 cgtaattaac atcctcaaca tgcttataga tcgtaatatc cagcattttt tcaacatatt
      181 ctggtccaca gtcatgacat atcgcaccca taaaagcttc aaaaatatcg gctaaaacac
      241 tatcacgatc acgcccacca ttattcaact cacctgctcc taaataaata agcgggccta
      301 gatcaagttc tctagcaaaa cgtgctaatg attcttcacg aacaagtttt gctctaagtt
      361 tggttagacg gccttctggc aattccgggt ataaatcaaa aatatgccga gaaacatgat
      421 actgtaaaac cgcatcacct aaaaactcca accgttcata atcatgattt ttcattttat
      481 tttcatttgc atatgaagga tgcgtaaaag cttccttaaa tatctaaaac agttttttcc
      541 agcatttctt ctaaaatatt tctatattct gataaggtat ttccattttt tgtaaaaatt
      601 gttctaattc cacgttaatt caatccttct ttcatttttt ccacaacgtc actagaaacc
      661 atttcaaaag ctaagttaat tgcattttca aaagcaattg catcacttga accatgggct
      721 ttaataaccg gtttaacaaa acccatcatt aaagcaccac ctgctgcctt agtatcaaat
      781 ttagtcaata actgttttaa agccggctta gcaagaaccg ctcctgcctt ggttctagtt
      841 gatgacataa aaccatcttt taaactttta accagcactt ttgcaacacc ttcaattgtc
      901 tttaatgcta cattacctgt aaagccatcc gttacgacaa catcaacttc accatccaaa
      961 atctctttac cttcaatatt accaacaaaa ttaatttctt gcatatcttc taacaattta
     1021 tatgtttctt gatgaatctc atcaccttta tgatgttcac ttccaatatt taataaagca
     1081 attttagggt tggtaatttt taaaacattt ttagcataag cgttacccat gattgcaaat
     1141 gatttcaact gttcagctgt attttctgaa tttgccccaa cgtcaagcat tgctacttta
     1201 ttatcactgt aagtaggcat cattgccatc aaacaagatt tttcaacacc ttcaatacgt
     1261 tttaaaaata acattgcact tgcataatag gcaccagtat ttccacaaga taagactgca
     1321 tcaacttcgc ccttacgagc cagcatcatt gttttaacca tacttgattc ctttttacga
     1381 cgaatggcta aaatattttc tgtcatttcc agaacttctc gcgcatcgat caaagtcact
     1441 tgatcatatg gttttaatgc ttctaattcc tcaatctttc ccacaacata taattcatct
     1501 gttttatttt tctctaaaaa tgatagacat gcttccacaa ctattctgct tcctaaatca
     1561 ccactcatgg catcaattcc taatttcatc tacttcacct catattagtc ataattataa
     1621 cataaattaa tcaatatatt tattattgtt gattaattgt tcttcgattt cttttattaa
     1681 ttttaaatat attaaatcat tagatttgct ttcaagcagt tctaaagcat cctttctaga
     1741 aatttctaaa atattagcat cttcgaaaat atttccaaca ctaaacaccg gtaatccact
     1801 ttgttgattt ccaagtatat cccccggtcc gcgcaatttt aaatcatagt aagaaacttc
     1861 aaaaccatca tgacaatttc ttaaaaattc taaacgttcc aatgcctctt gacttgaaga
     1921 attacttaat aaaaaacaat atccctgctg atcactacga ccaacacgac cacgcagctg
     1981 atgaatctga ctcaatccaa atcgctcagc attgtaaata acaatccaat ttgcattact
     2041 tacatctacc ccaacctcaa ttacagttgt tgaaactaag atttgaatct cattagcttt
     2101 aaatgctgcc atgatctgat cctttgtttc atcgtccatt ttaccatgaa gcagaccaat
     2161 ttgatactgt tctttaaaat aggccttcat cccattatat attcccgtag catccctgct
     2221 atcgatagct tcactttctt caaccagagg gcagacaaca taacattgtc ctccacttgc
     2281 taaatactct ttcaaacggt ttaaaatagg ttt
//
LOCUS       DAOMTA010000504         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2182_length_2309_cov_2.053682, whole genome shotgun sequence.
ACCESSION   DAOMTA010000504 DAOMTA010000000
VERSION     DAOMTA010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2182_length_2309_cov_2.053682"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLK68_12110"
     CDS             complement(<1..368)
                     /locus_tag="PLK68_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRM91188.1"
                     /translation="MKRLVLASSSPRRKELLELHKFDFIIDFQEIEEVLDESLALPLR
                     LEKLAYQKAAPIALKYPSDIVIGADTMVCLENQMLGKAADRQAAYEMLKLLSDQTQTV
                     YSAVAIIDNGKVSTYHDGTKV"
     gene            complement(368..>2309)
                     /gene="pnp"
                     /locus_tag="PLK68_12115"
     CDS             complement(368..>2309)
                     /gene="pnp"
                     /locus_tag="PLK68_12115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRM91189.1"
                     /translation="GKIPGGFLKREGRPSEHGTLTARMIDRPIRPLFADGFRNEVQSV
                     NTVLSVDQDATPEMAAMLGASLALCVSDIPFNGPIAGVNVGLVDGEFIVNGTPEQLEN
                     SLINLEVAGTKDAINMVEADAKEVSEEVMLEALLFGHEKIKELIAFQEKVVEACGKEK
                     IEIPLFELNNDLVEEVAVRAKAEMAAAVSIPGKLERYGAIDDLIAQVTEEYDNREYEN
                     EEIKADVMKQVGIILHDLEKDEVRRLITEDKIRPDGRKIDEIRPLDSQIDLLPRVHGS
                     ALFTRGETQVLSVATLGAIGEHQKIDGLGLEDQKLFMHHYNFPPYSVGETGRMGAPGR
                     REIGHGALGERALAQVIPSEEAFPYTIRVVSEVLESNGSSSQASICASTMALMAAGVP
                     IKAPVAGVAMGLVKKGDAYTILTDIQGMEDHLGDMDFKVAGTDAGICALQMDIKIDGI
                     TKEILQEALAQAKVARKQIMANMMDAIAAPRDELSPYAPKVQMMKIEPDQIKAVIGQG
                     GKTINEIIEQSDGVKIDIEQDGTVVIYHYDQAAINKAVELIEKIVKKAQVGEVYDGKV
                     VRVEDNYAFINLFDGTDGFLHISDYAYERTKKMGEVIKLGDIIKVKVTKVDDKGKVNV
                     SRKALLPKPIKKEEPKEEPKAE"
BASE COUNT          663 a          522 c          313 g          811 t
ORIGIN      
        1 actttagttc catcatggta agtagaaact tttccattat caatgattgc taccgctgag
       61 taaacagttt gggtctgatc agataacaat tttaacattt cataagctgc ctgacgatca
      121 gcagcttttc ccagcatctg attttctaaa cataccatag tatctgctcc aataacaata
      181 tcactagggt attttaaagc aatgggtgct gctttttgat aagctaactt ctctaaacgt
      241 aaaggaagtg ccaaactttc atctagcact tcctcaattt cttgaaagtc gattataaaa
      301 tcaaacttat gtaattctaa taattctttt cttctaggtg aactactagc aagaacaagc
      361 cttttcatta ttctgccttt ggttcctctt taggttcttc ttttttgatt ggtttaggaa
      421 gtaaagcctt gcgtgaaaca tttacttttc ccttatcatc aactttagtg actttaactt
      481 taatgatatc acctaatttt atcacttcac ccattttttt agttctttca tatgcataat
      541 cagagatatg caaaaaacca tctgttccgt caaacaaatt aataaaagca taattatctt
      601 ctactcttac tactttacca tcataaactt cgccaacttg cgctttttta acaatttttt
      661 caattagctc aactgcttta ttgattgcag cttgatcata atgataaata acaactgtac
      721 catcttgttc aatgtcgatc ttaacaccat cagattgttc aatgatttca ttgattgttt
      781 tacccccctg accaataacc gctttgattt gatccggttc aatcttcatc atttgtactt
      841 ttggtgcata tggtgataat tcatcacgtg gtgctgcaat cgcatccatc atatttgcca
      901 taatttgttt acgagctact ttagcttgag ctaaagcctc ttgtaaaatt tctttcgtaa
      961 taccatcaat cttaatatcc atttgtaatg cacaaatacc cgcatcagtt cctgcaactt
     1021 taaaatccat atctcctaaa tgatcttcca ttccttggat atctgtcaag atagtatatg
     1081 catccccttt ttttactagt cccatagcta ctccagctac tggtgcctta attggaacac
     1141 cggctgccat taaggccatt gtcgaagcac agatagaagc ctgtgaagag gaaccattag
     1201 attccaatac ttcagaaacc actctaattg tataaggaaa agcttcttca ctaggaatca
     1261 cttgtgctaa agctctttcg cctaaagcgc catgcccaat ttcacgacga cctggagcac
     1321 ccattcgtcc tgtttctcca actgaatatg gcgggaaatt ataatgatgc ataaaaagtt
     1381 tttgatcttc taaccctaat ccatctattt tttgatgttc tccaattgca cctaatgttg
     1441 caaccgataa aacttgcgtt tcaccacgtg taaataacgc tgacccatga actcgaggta
     1501 acaaatcaat ttgcgaatct aatggcctga tttcatcgat tttgcgtcca tccggtctta
     1561 ttttatcttc agtaattaat cgacgtactt catctttttc tagatcatgt aaaatgattc
     1621 caacttgttt catcacatca gcttttattt cttcattttc atattctcta ttgtcatatt
     1681 cctctgtcac ttgagcgatt agatcatcaa ttgctccata tctctctaat ttaccgggaa
     1741 ttgaaactgc tgccgccatt tcggctttag cacgtactgc aacttcttca actaaatcat
     1801 tatttaattc aaataatgga atttcaattt tttcttttcc acaagcctca actacttttt
     1861 cttggaaagc aattaattct ttgatttttt catgaccaaa caataatgct tccaacatta
     1921 cctcttcact aacttcttta gcatcagctt caaccatatt gatagcatct ttggttccag
     1981 ctacctctaa atttattaaa gaattctcta gctgttctgg tgttccgtta acaatgaatt
     2041 caccatcaac caaaccaacg ttgacccctg caatagggcc attaaaaggt atatcagaaa
     2101 cacataaagc caatgatgca cctaacatag ccgccatttc tggagttgca tcttgatcta
     2161 cagataaaac ggtgttaaca ctttgcactt catttctaaa cccatctgca aataatggtc
     2221 taattggacg gtcaatcatt cgagctgtta atgtcccatg ttcactaggt cgcccttctc
     2281 tttttaaaaa tccacctgga atctttcca
//
LOCUS       DAOMTA010000505         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2183_length_2309_cov_1.872671, whole genome shotgun sequence.
ACCESSION   DAOMTA010000505 DAOMTA010000000
VERSION     DAOMTA010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_2309_cov_1.872671"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PLK68_12120"
     CDS             <1..1497
                     /locus_tag="PLK68_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM91190.1"
                     /translation="TCLIQSSSGTTALAIGLIRSGLMTLEQSIGIIMGANIGTVITSV
                     FVGLKVSQYAVYFIILGAAFLMFSKNKKTKYMGQIIFGFGCLFYGLELMGDNLANISK
                     VPEFTQVANYLSQNPWLGLLGGTLLTTAVQSSAAVIAIAQQMYGAGAIGLSIALPFLF
                     GSNIGTTITAVLASFGGTVPAKRAAFFHVLFNVVGSLLFMIVLSPFTLLIEWLAGALN
                     ILPELQLSVAHGIFNVVTTAFFFPLIPAIVKLIKKILPSSKKEINMDLSELDQNVVQL
                     FPSHALAIAKNKIIEMGHITIEAVEGIRAYFETKNPLAKDGVYEMENAINTLDSKITE
                     YLVLISHETLNDHDSNDYLANMKTIKDFERIGDLCINIVKYYEAIYDEKEDFSPEARE
                     DLEAMMDMVIDMLNHAVKAFDTHDLDDIVYVDDKEADLDYFNKKAKQRHIKRVGRKIE
                     NSALVNSTYVDILANLERMGDHCQNISESYLLDESAYLNEEPESAFSK"
     assembly_gap    1702..1739
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1740..>2309
                     /locus_tag="PLK68_12125"
     CDS             <1740..>2309
                     /locus_tag="PLK68_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HRM91191.1"
                     /translation="IFVPQKKSSKNNREQRDQWLLNYVNHGYEFIGKSRKIYVFKAAQ
                     AANIKGTDQALLLTYFKRNKTISNIIFIFVAMLLSFLLIPSVFANQNPMEFITNGSII
                     LHYIPLLFCPALLIRFFNHHLTTEKIKLTLSNKKANNKISKYPFIMSNWLLIVSIVLI
                     IAGFSIDFIGRETLPLNDRIITLSALGLSS"
BASE COUNT          777 a          347 c          376 g          771 t
ORIGIN      
        1 acttgtttga ttcagtcaag ttcaggaaca acagcattag caattggttt aattcgttct
       61 ggtttgatga ccctcgaaca atcaattgga ataattatgg gtgccaacat cggaacagtt
      121 attacgtccg tatttgtagg gttaaaagta tctcaatatg cagtatactt tattatcctt
      181 ggggcagctt ttttaatgtt ttcaaaaaac aaaaaaacaa aatatatggg acaaatcatc
      241 tttggatttg gatgtttatt ctatggttta gagttaatgg gagataattt agcaaatatt
      301 tcaaaagtcc cagaatttac acaagtagcg aattatctat cacaaaatcc atggttagga
      361 ttattgggtg gaacattatt aacaacagca gttcaatctt cagcagcggt aattgcaatt
      421 gctcaacaga tgtacggtgc tggagcaatc ggtttatcta tcgctttacc attccttttt
      481 ggaagtaaca tcggtacaac gattactgcc gtattagctt catttggtgg aacagtacca
      541 gctaaacgtg ctgctttctt ccatgtatta ttcaatgtgg ttggatcact attatttatg
      601 atcgtcttat cgccattcac gctcttgata gaatggcttg ctggagcatt aaatatttta
      661 cctgagctac aattatcagt agctcatgga atctttaacg ttgtaactac ggcattcttt
      721 tttcctttaa taccagcaat cgtaaaactt atcaaaaaaa tcttaccgag cagtaaaaaa
      781 gaaataaata tggatttaag cgaattagat caaaatgttg ttcagttatt cccgagtcat
      841 gctttagcta ttgctaaaaa caagataatc gagatgggtc atattacaat tgaagcagtt
      901 gaaggaatta gagcttattt tgaaactaaa aacccattag ctaaagatgg tgtttatgaa
      961 atggaaaatg ctattaatac actagacagt aaaattactg agtacttagt tttgatttcg
     1021 catgaaactc taaatgacca tgattctaat gactatttag caaatatgaa aactattaaa
     1081 gattttgaaa gaattggtga tttatgtatt aacattgtta aatattatga agcaatctat
     1141 gatgaaaaag aagatttttc accagaagct cgtgaagatc tagaagcgat gatggatatg
     1201 gtcattgata tgttaaatca cgcagttaaa gcctttgata cacatgattt ggatgatatt
     1261 gtttatgttg acgacaaaga agcagattta gattacttca acaaaaaagc taaacagcgt
     1321 catatcaaac gagttggtcg taaaattgaa aattcagcac tagttaattc aacttacgtc
     1381 gatattcttg ctaatctcga acgaatgggc gatcattgtc aaaatatttc tgaatcgtat
     1441 ttattagatg aatcagccta tcttaacgaa gaaccagaat cagcattttc aaagtaaacc
     1501 gtgttttaca cggtttttct ttattgtttg gataaaaaat gataatctaa taatatattt
     1561 ataaggagtc gattatgaaa ttaagattaa ctaattatga tactagtgag tataaacaat
     1621 ttgaagatat gctaaataat ttatcaaagt caggctataa ttgcaaaagc gttgatatgt
     1681 ttacagtttt taaaaaagat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1741 tttttgtacc tcaaaaaaaa tcttctaaaa acaatcgtga acagcgtgat cagtggttat
     1801 taaattatgt aaaccacggt tatgaattta ttggtaaatc acgaaagata tatgttttta
     1861 aagccgctca agcagccaat attaagggta cagatcaagc actgctgcta acttatttta
     1921 aacgcaataa gaccatctcc aatattattt ttatttttgt cgctatgctg ctttcttttt
     1981 tactgattcc aagtgttttt gctaatcaaa acccaatgga atttatcacg aacgggtcaa
     2041 taattttaca ttatattccc ttattatttt gcccagcatt actaatccgt ttcttcaatc
     2101 atcatctcac tactgaaaag atcaaactaa ctttaagtaa taaaaaagca aataataaaa
     2161 ttagcaaata tccatttatt atgtcaaatt ggttactaat tgtttcaatt gttttaatta
     2221 ttgctggatt ttcaatcgat tttatcgggc gtgagacact cccgcttaat gataggatca
     2281 ttacattatc agcacttggt ctatctagt
//
LOCUS       DAOMTA010000506         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2187_length_2305_cov_2.528000, whole genome shotgun sequence.
ACCESSION   DAOMTA010000506 DAOMTA010000000
VERSION     DAOMTA010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2187_length_2305_cov_2.528000"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLK68_12130"
     CDS             complement(<1..607)
                     /locus_tag="PLK68_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM91192.1"
                     /translation="MKVNAKEEFAYTYTRDGNPTLNLLETKLARLEKGEAAQMFASGM
                     GAISASILTLAKAGDHIIIVNTVYGSSVKLIKQLSKFGIESTKIDVSDTLEIFDYVKN
                     NTSIIYFESPSSQKFEMLDLELISKFSKDKGIFTIIDNTWSTPLLQNPLVHGIDVVIH
                     SCSKYIGGHSDIVGGVVISSKKIIDEIVEFGQVLLGATMSPM"
     gene            complement(591..725)
                     /locus_tag="PLK68_12135"
     CDS             complement(591..725)
                     /locus_tag="PLK68_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91193.1"
                     /translation="MKDIENVCLEVDELDFDNYSPISPDIVLTSSFKFKNFDHYESEC
                     "
     gene            complement(825..1850)
                     /locus_tag="PLK68_12140"
     CDS             complement(825..1850)
                     /locus_tag="PLK68_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRM91194.1"
                     /translation="MKSAVFYGKHDIKVEEVEMPQLGNQDVLIKVMACGICGTDVHIY
                     EGDKGAANTTPPTILGHEFAGIVEAVGHEVKHVKVGDRVCVDPNQLCGTCYYCRSGIG
                     HFCEDMIGIGTTRDGGFAQYCAVNESQVYKLADTTSFEEGAMTEPVACSLHGLDMCNI
                     TPASTVLVIGGGMIGLLMVQLAKLAGAHEIILSEPVAVKREMGLKMGATLTVDPINDN
                     IPALLADKGIHRINTVIECAGLPATIKQAISLAGNKSVVMMFGLTKPDDEVAIKPFEI
                     FQKEIEIKASFINPYTQQRALDLINSKKIDVSSMVCDICSLDKLADILSKPELRNKGK
                     YIINPWQ"
     gene            complement(2026..>2305)
                     /gene="queF"
                     /locus_tag="PLK68_12145"
     CDS             complement(2026..>2305)
                     /gene="queF"
                     /locus_tag="PLK68_12145"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRM91195.1"
                     /translation="NQKMVESKSLKLYLFSFRNHGDFHEDCMNIIMKDLIKLMDPKYI
                     EVWGKFTPRGGISIDPYCNYGKPETKWETIAFNRLADHDLYPEKIDNR"
BASE COUNT          737 a          426 c          336 g          806 t
ORIGIN      
        1 tcattggtga catcgttgcg cctagtaaaa cttgtccaaa ttcaactatt tcatcaataa
       61 tttttttgct ggaaattacg actccaccga ctatatcact atgtccacca atatatttgg
      121 aacatgagtg aataacaaca tcaattccat gaactaatgg attttgtaat aatggtgttg
      181 accaagtatt atcgattatc gtgaagatac ctttatcttt gctaaattta ctgattagtt
      241 ctaaatctaa catctcaaat ttttgtgatg atggactttc aaaatagatg atactggtat
      301 tgtttttgac ataatcaaag atttctaaag tatcactgac atcaatttta gtactttcga
      361 ttccaaattt tgataattgt ttgattaatt taactgatga gccatagact gtattaacaa
      421 tgataatgtg atcaccggct tttgctaagg ttaaaattga tgctgaaata gcgcccatcc
      481 ctgaggcgaa catttgagct gcttcgcctt tttctaaacg tgctaatttt gtttctagta
      541 aattaagtgt tggattcccg tcccgtgtgt atgtataagc aaattcttct ttagcattca
      601 ctttcatagt ggtcaaaatt tttaaattta aatgatgatg ttaaaacaat atctggacta
      661 attggactat agttgtcgaa atctaactca tcaacttcta aacatacatt ttcaatatct
      721 ttcatattcg ttctccttac agttaaatct taaccgacaa aatggcgaat gtccattttt
      781 tttcatatat ggcaaaaaaa gaatgtacat aaaattgtac attcttattg ccatgggtta
      841 ataatatatt tacctttatt tcttaattct ggttttgaaa gaatatcggc taatttatct
      901 aaactgcaaa tatcacaaac cattgaacta acatcgattt ttttactatt gattaagtct
      961 aaagctcgtt gttgagtata tgggttgatg aaagaagctt taatttcaat ttccttttgg
     1021 aaaatttcaa atggtttgat agccacttca tcatcaggtt tagttagccc aaacatcatt
     1081 actacagatt tattaccagc taagctgatt gcttgtttaa ttgttgcagg tagaccagca
     1141 cactcgataa cagtgttgat acgatgaatt cctttatcag ctaaaagagc tggaatattg
     1201 tcattgattg gatcgacggt taaagtagca cccatcttaa gccccatttc acgtttaaca
     1261 gcaactggct ctgacaagat aatttcatga gcaccagcta attttgctaa ttgaaccatt
     1321 aacaaaccaa tcatgccacc accaataaca agaactgtac tagccggagt aatgttgcac
     1381 atgtcaagac catgtaagct gcaggcaact ggctcggtca ttgccccttc ttcaaatgat
     1441 gttgtatcgg ctagtttata tacttgagat tcgttgactg cacaatattg ggcaaaacca
     1501 ccatcccgtg ttgttccgat gccaatcata tcttcacaaa aatgaccaat accactacga
     1561 caatagtaac aagttccgca tagttgatta ggatccacac agacacgatc accaactttt
     1621 acatgtttta cttcatgtcc cacagcttcg acgattccag caaattcatg tcctaaaata
     1681 gtaggaggag tagtatttgc tgctccttta tcaccctcat aaatatggac atcagtacca
     1741 caaattccac aggccataac tttgattaat acatcttgat tacctaattg tggcatttct
     1801 acttcttcaa ccttaatatc atgttttccg taaaatactg cactcttcat ttttcttcct
     1861 ccagacatct tatataaaat agttttataa aagtatttaa tgtaagtata aatgatgatt
     1921 aatatgatgt caacggtttt aaaatatatt attttttgag ctaactcttt gatgtaaagt
     1981 tgaaaacaaa ttagatacct tgaaaaaggt ctctaataat tatatttaac gattatcaat
     2041 tttctcagga tataaatcat gatcagctaa acggttgaaa gcaatggttt cccactttgt
     2101 ttctggtttt ccataattac aatatggatc aattgaaata cctccacgtg gtgtaaattt
     2161 tccccaaact tcaatgtatt taggatccat taatttaatc aggtctttca taatgatatt
     2221 catacaatcc tcgtgaaaat caccatgatt cctaaaactg aataaataca gttttaatga
     2281 tttactctct accatctttt gatta
//
LOCUS       DAOMTA010000507         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2188_length_2305_cov_2.289778, whole genome shotgun sequence.
ACCESSION   DAOMTA010000507 DAOMTA010000000
VERSION     DAOMTA010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2188_length_2305_cov_2.289778"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(524..841)
                     /locus_tag="PLK68_12150"
     CDS             complement(524..841)
                     /locus_tag="PLK68_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRM91196.1"
                     /translation="MLEKVREEIIKFNQDRDWDQFHSPENLAKSIAIESGELLECFQW
                     DNSFNKQDVCDELADVVNYCILMADKLDVDLEDIVLKKLKKTEKKYPVEKAKGNSKKY
                     NQL"
     gene            complement(825..>2305)
                     /locus_tag="PLK68_12155"
     CDS             complement(825..>2305)
                     /locus_tag="PLK68_12155"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HRM91197.1"
                     /translation="YVSLIEDYNEDVRKYKINLQPCAYLHNYRKKEYDDLVDLCYEYY
                     LDKAPVFTRGDTKKLAMFIARYVKKGNPDVMYHIDSGKIKPSKSLQDSLTNMLKGKPE
                     FTLLDEQKVTYEKALAIAKENSARKQVYIIHGGPGTGKTVIAVNMLVELINRDKNTIY
                     VTKNSAPREVYQKKLTDGGYKKVYINNLFKGSGVFINANENDFDCIIVDESHRLNAKS
                     GMYQNNGENQIKEIINAAKFSIFFVDDHQIVTTSDIGSSKEIKKWCKYYNAIVYEDKL
                     TSQFRCNGSDAYLSWVDNVLEIEDTANDELDIDYDVRIFDDPLALKKAIFEKNKIANK
                     SRMLAGYCWKWQKNGRNRSDVHDIEIGDFSMSWNFASTKTWAIDEESINEVGCIHTSQ
                     GLEFDYVGIIIGDDLRYENGKIITDFFKRAPTDRSIRGLKGQYKNDKESALKLADEII
                     KNTYRTLLTRGQKGCYIYCTNKELNEYLKLKLKRGKENVRKS"
BASE COUNT          744 a          419 c          209 g          933 t
ORIGIN      
        1 ctaccttttt taattacttt ttcaattgtt tctggatata attctggatt taattcttta
       61 tacttctcat tgtatttttt aggatgcgtt cccttatatc taatccttct tttatgttta
      121 ggttccataa ttagattcct ctctttttta gctactaatt ttttatttac taacaactat
      181 cttatcatca ttagatagta aatgtctagg atatatgcaa atcatctatg cttactgaga
      241 taatcaaaaa tatttattaa gtaatttaga aaattttata taaaaaggat aaaatgtttt
      301 aaaactaacc ttttatcctt tcttcaaaat tatcctcttt atataatttt ttcatttcta
      361 ctgcagtatt attacccaaa taaaatgcaa tgggtagaac tctcaatttt tttaatttta
      421 agtaacgatt ttaatactct ttaaattcct catatcaaaa tttattgata tcttttaata
      481 taaaacagca tatttataca acactttatt atatatacaa agcttataat tgattatatt
      541 ttttactatt tcctttagct ttttcaacag gatatttctt ttcagtcttt ttcaatttct
      601 ttaatactat atcttccaaa tcaacatcta acttatcagc catcaaaata caataattta
      661 ccacatcagc caattcatca catacatctt gcttattaaa gctattatcc cattgaaaac
      721 actctaataa ttcacctgat tcaattgcaa tacttttcgc taaattctct ggagaatgaa
      781 attgatccca atccctatcc tgattaaact taattatctc ttctctaact ttttctaaca
      841 ttctctttac ctcttttaag ttttaatttt agatattcat ttaattcttt gtttgtacaa
      901 tatatatagc atcctttttg tcccctagta agaagtgttc tatatgtatt ctttataatt
      961 tcatctgcta attttagagc actttcttta tcatttttat attgaccttt taaaccacga
     1021 attgatctat ccgtaggtgc ccgtttaaaa aaatctgtaa taatcttgcc attttcataa
     1081 cgtaaatcat ctccaataat gattcctaca taatcaaatt ccaatccttg acttgtgtgg
     1141 atacacccta cttcgtttat agattcctca tcaattgccc aagttttagt tgatgcaaaa
     1201 ttccatgaca tcgaaaagtc tccaatttca atatcgtgaa catctgatct atttctacca
     1261 tttttttgcc atttccaaca atagcccgct aacattcttg atttattagc aattttattc
     1321 ttttcgaaaa tagctttttt taatgctaaa ggatcatcaa agattctaac atcataatca
     1381 atatccagtt catcatttgc agtgtcttct atctctaaaa cattatctac ccacgataaa
     1441 taagcatcac tgccattaca tctaaattgc gaagtcagct tatcctcata aacaatagca
     1501 ttataatatt tacaccattt ttttatttct ttacttgaac caatatcact ggttgttaca
     1561 atttgatgat catcaacaaa gaaaattgaa aacttggcag cattaataat ctccttaatt
     1621 tgattttctc cattattttg atacattcca gattttgcat ttaagcgatg tgactcatca
     1681 actattatac aatcaaaatc attttcattc gcatttataa aaacacctga acctttaaat
     1741 aaattattaa tataaacctt tttatatcca ccatcagtta gtttcttttg atatacttca
     1801 cgaggtgcag aatttttagt tacataaata gtatttttat ctcgattgat caattcaact
     1861 aacatattaa ctgcgataac tgttttacca gttccaggac caccatgaat aatataaact
     1921 tgttttctag cagaattttc tttagcaata gcaagagctt tttcataagt aactttttgt
     1981 tcatctaaca aggtaaactc tggtttaccc tttaacatat tagttaaact atcctgtagt
     2041 gatttactcg gcttaatttt accactatca atatgataca ttacatcagg attgcccttt
     2101 tttacatatc tagcaataaa cattgctaat tttttagtat caccacgggt aaaaacaggg
     2161 gctttatcta aataatattc ataacataag tctactaaat catcatattc tttctttcga
     2221 tagttatgta aataagcaca tggctgcaaa ttaattttat attttcttac atcttcatta
     2281 taatcttcaa ttaaactaac atatg
//
LOCUS       DAOMTA010000508         2299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2206_length_2299_cov_2.375668, whole genome shotgun sequence.
ACCESSION   DAOMTA010000508 DAOMTA010000000
VERSION     DAOMTA010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2206_length_2299_cov_2.375668"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..777
                     /gene="kdsB"
                     /locus_tag="PLK68_12160"
     CDS             34..777
                     /gene="kdsB"
                     /locus_tag="PLK68_12160"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRM91198.1"
                     /translation="MKFIGIIPARYASTRFPGKPLALLAGKPVIQRVYEQVKSVLDDV
                     YVATDDRRIYDAVKSFGGNVVMTSAHHKSGTDRIEEAVKNIGGDFDVVINIQGDEPFI
                     QSSQIKTVCECFNDSETQIATLGKPFTQDMGMEAIENPNSPKIVIDNRNYAMYFSRSI
                     IPFIRGKEKTQWLESYPFVKHIGLYAYRRHVLSEITSLPQSSLEKAENLEQLRWLQNG
                     YRIKVGLTEIETIGIDTPEDLKKAEAFLS"
     gene            complement(769..1350)
                     /locus_tag="PLK68_12165"
     CDS             complement(769..1350)
                     /locus_tag="PLK68_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM91199.1"
                     /translation="MTIDQQQAIKRFAEIGLTEVEKHLDPYTQKGIFGDLEEGKISAD
                     EFRIKLGNIIGKELTFKECEYAWRGYFKELPQRNLDVLVKLKEKGYNLILLSNTNPFI
                     MHWGLSENFDGVGHSLNYYFDALYLSYKNKMMKPDEKFFYKMLITEKIQPHETIFVDD
                     GPRNVAVASKLGINTFCPKNGIDWTEDIYNYLT"
     gene            complement(1407..>2299)
                     /locus_tag="PLK68_12170"
     CDS             complement(1407..>2299)
                     /locus_tag="PLK68_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRM91200.1"
                     /translation="DCLINLADKIKEEQNLVNNGRDTTTASPAKIDSWRNDSLPERLE
                     YSLMKGIGEYIDVDIHEALKTYNNAVQIIEGPLMSGMNKVGELFGSGKMFLPQVVKTA
                     RTMKQAVAILQPYIEAEKKDGATKSGKVLLATVKGDVHDIGKNIVGVVMACNNYEIID
                     MGVMVPAEQIVRKAIEEHVDIIGLSGLITPSLEEMVHVAQEMRKANLNIPIMIGGATT
                     SELHVALKIAPVYNGPVVWMRDASQNALVAPKLINDNERTRIKENLDKKYENLCEEYR
                     EEQQKIISIEESRKNKLNLF"
BASE COUNT          721 a          434 c          338 g          806 t
ORIGIN      
        1 tatgtaactt tgcgtcgata aattaatatt gaaatgaaat ttataggaat tataccagct
       61 agatatgctt ctacacgttt tccaggaaaa ccactagcct tattagcagg caagcctgtt
      121 atacagagag tttatgaaca agttaaatcc gtattggatg atgtctatgt agccacggat
      181 gatcgtagaa tttatgatgc tgtaaaatct tttggaggaa atgttgttat gacctcagca
      241 catcataaaa gtggtaccga ccgcattgag gaagccgtaa aaaatatagg cggagatttc
      301 gatgtagtaa tcaatattca aggggacgaa ccttttatac agtcttcaca aataaagact
      361 gtctgtgaat gttttaatga tagcgaaaca caaatagcaa ctcttggtaa accatttaca
      421 caagatatgg gcatggaggc tattgaaaat cccaattcac caaagatagt aatagacaac
      481 agaaattacg caatgtactt tagtagaagt ataataccgt ttatacgtgg aaaagaaaag
      541 actcaatggt tagaatcgta tccatttgtt aagcatatag gtttatatgc ttataggcgg
      601 catgttcttt ctgaaataac tagccttccg caatctagcc ttgaaaaggc tgaaaatttg
      661 gagcaattac gatggttgca aaatggatac cgaatcaaag taggccttac tgagatagaa
      721 acaataggga tagatacccc tgaagatctc aaaaaggcag aagccttctt aagttaaata
      781 attatatata tcttctgtcc aatcaatacc attcttaggg cagaaggtat ttattcctaa
      841 tttacttgca actgcaacat tgcgtggtcc atcgtcaaca aatattgtct catgaggttg
      901 aattttttct gtaatgagca tcttatagaa aaatttctcg tctggtttca tcattttatt
      961 tttataacta agatataaag catcaaaata ataatttaaa gaatgcccta ctccatcaaa
     1021 gttttcactt aatccccaat gcataataaa aggatttgta ttagaaagca atattaaatt
     1081 atatcccttt tcttttaatt taactaaaac atctaagttc ctctgcggaa gctctttaaa
     1141 atatccgcgc catgcatatt cacactcctt aaatgtaagt tctttaccaa taatattacc
     1201 tagttttata cggaattcat ctgcactaat ttttccttct tccaaatctc caaaaatgcc
     1261 tttttgcgta tatggatcaa gatgcttttc aacttcagtt agtcctattt cggcaaatct
     1321 tttaatagcc tgttgctgat ctatagtcat aataacacca ccaagatcaa aaactatatt
     1381 tttaatttta ctaccaataa tcataattaa aataaattca atttgttttt tctagattct
     1441 tcaatagata ttattttctg ttgctcttct ctatattctt cacaaagatt ttcatatttt
     1501 ttatcaaggt tttctttaat ccttgttctt tcattatcat ttataagttt tggagcaact
     1561 aatgcattct gacttgcatc tctcatccat acgacagggc cgttatatac tggtgctatc
     1621 tttagtgcta catgcaattc actagtagtt gcaccaccta tcattatagg aatgttaaga
     1681 ttagctttac gcatttcttg ggcaacatgt accatctctt caaggctagg tgttataaga
     1741 ccacttaatc caattatgtc tacatgttct tcaattgctt tcctaacgat ttgctcagct
     1801 ggaaccataa ctcccatgtc tattatctca taattattac atgccatcac tactccaact
     1861 atatttttac caatatcatg cacatctcct tttactgtag ccaataagac tttgcctgat
     1921 ttagtcgcac catctttttt ttcagcttca atatatggct gtaatattgc tacggcttgt
     1981 ttcatagtac gagcagtttt aactacttgt ggcaagaaca tttttcctga tccaaaaagt
     2041 tcaccgactt tattcattcc gctcattaat ggtccttcta ttatctgaac cgcattatta
     2101 taagtcttta gtgcttcatg gatatcaaca tcaatatatt ctcctatacc tttcataagt
     2161 gaatattcca aacgttctgg caatgaatca tttcgccatg aatcaatttt tgcaggactt
     2221 gcagttgttg tatccctgcc attatttact aaattttgtt cctcttttat tttatccgct
     2281 aagttaataa gacagtcct
//
LOCUS       DAOMTA010000509         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2209_length_2295_cov_2.152232, whole genome shotgun sequence.
ACCESSION   DAOMTA010000509 DAOMTA010000000
VERSION     DAOMTA010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_2295_cov_2.152232"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1158
                     /locus_tag="PLK68_12175"
     CDS             292..1158
                     /locus_tag="PLK68_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91201.1"
                     /translation="MIKRLNKNFFAAILTFAMAIGMIAPVNVAANDEYLNFDQDSIYA
                     IVSTTTGKALNIKTASWTSPAEADGEYNAKTNKISNSSQFMFINENDGSVTIKYTGIN
                     DGQDYYLRTESGQTYVWADTRNEDTTAKYKITKTADNQGIIESMKRTNEYLSIDSKGK
                     LVYTSDRDLAEKFNFVKNPGIISNIAWIENVATGKLVTFADQPDENFSAIKVTGDAGN
                     IGDTEKFKVTFHTNDNGIKNVISFESVSKPGYQIASAKWVDGAVPTIGSYKKVGGGKL
                     SQLNRLEMDCLY"
     gene            1146..>2295
                     /locus_tag="PLK68_12180"
     CDS             1146..>2295
                     /locus_tag="PLK68_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRM91202.1"
                     /translation="MFVLKDAATGKLVTANNDEVLEGGYEGELTDREKFIIHNAIDIA
                     DVTDLKADDSSRTETTIDLTWTNPNSLYTDVTLFKKASNEVVFDKVADVTGKNSYQVT
                     DLKAGMQYSFKLEYVNGNGNLSDINNPKNESAELKVSTRAGAKPATPADVKLVEKDGE
                     FTISWGAAENATHYQLLRADSMLGEYNVVETVTRDSTSVTVAPIGDKYENYYRIIALN
                     NGQNNDDDLSNAEISERSEYVSLEKNIFGEHTLFFSPKDDVAQLDKILLDLFNQQNDR
                     GADAQFKGQQYQVYFKPGDYTETTCMYLGFYTSFNGLGKIPMDVKLNNIAIPSYLDGN
                     NATCNFWRSAENLAVINTGNEQGKAGFDCNLARPEYFNWAVAQAAPLRR"
BASE COUNT          845 a          296 c          448 g          706 t
ORIGIN      
        1 ttataatgtg gaaaactact aaaaaataag tagttttttt tataaaaaaa taagcaaact
       61 tgttacattt aatgtaaccc gttacataac ctcttgcgtt cattccttct tattattaat
      121 tatgttattt tataggtgta atgttaagca cattgcattg aatatcaaat aatcattaag
      181 cgcttatagt atttgatatt ctaattgtta tggattgtat tgaagtaggc cgctttgata
      241 caattcaaat attgcttata tacgaaatat attaagaagg ggaaatggaa aatgataaaa
      301 agattaaaca agaatttttt tgcagctatt ctgacctttg cgatggcaat tggtatgata
      361 gcacctgtaa atgttgcagc gaatgacgaa tacttgaatt ttgatcagga ttcaatttat
      421 gcaattgttt caactacaac tggaaaagca ttaaatatta aaacagctag ctggacatca
      481 cctgcagaag cagatggtga atataatgct aagacaaata aaataagtaa ctcatcacaa
      541 tttatgttta ttaatgaaaa tgatggttca gtaacaatta agtatactgg tataaatgat
      601 gggcaagatt attacttaag aacagagtct gggcaaacat atgtatgggc tgatactaga
      661 aatgaagata ccacagctaa gtataaaata actaaaacgg ccgataacca aggtattatt
      721 gaaagtatga aaagaactaa tgaatatctt tcaattgata gtaaaggaaa attagtctat
      781 acaagtgata gagatttggc tgaaaaattt aattttgtca aaaatcctgg aattatcagt
      841 aatatcgctt ggattgaaaa tgtggctaca ggaaagttag taacatttgc tgatcagcca
      901 gatgaaaact tttctgcaat taaggtaact ggagatgctg gtaatatagg tgatactgaa
      961 aagtttaaag ttacttttca cactaatgac aatgggatta aaaatgtaat ttcatttgaa
     1021 tcggttagta aaccaggtta tcaaattgca tcagctaagt gggtagatgg cgctgtacct
     1081 accattgggt catataaaaa agtgggtggt gggaagctat cgcagttgaa ccgattggaa
     1141 atggattgtt tgtattaaag gatgcagcta caggaaaatt ggttactgca aataatgatg
     1201 aagttctaga aggtggttat gaaggggaat taactgatcg agaaaaattt attatccata
     1261 atgctattga tattgctgat gtcacagatt taaaagctga tgatagctca agaactgaaa
     1321 ccacaattga tcttacttgg acaaatccga atagtttata tactgatgta accttattta
     1381 aaaaagcttc aaatgaagta gtgtttgata aagttgctga tgtaactgga aaaaacagct
     1441 atcaagtaac tgatttgaaa gcggggatgc aatatagttt taaattagaa tatgtaaatg
     1501 gaaatggaaa tttatcagat attaataatc cgaaaaatga aagtgctgaa ttgaaagtta
     1561 gtacaagagc aggagcaaaa ccagcgacac cagcagatgt taaattagtt gaaaaagatg
     1621 gtgagttcac aattagctgg ggagctgctg aaaatgcaac acactatcaa ttgttaagag
     1681 cagatagtat gcttggggaa tataatgtcg tagagactgt aactagagat agtacttctg
     1741 ttactgttgc acctataggt gataaatacg agaattatta tcgcatcatt gctctaaata
     1801 atggacaaaa taatgatgat gatttatcaa atgctgaaat ttcggaacgt tctgagtatg
     1861 tatctctaga aaagaatata tttggagaac atacgttgtt cttctcaccc aaagatgatg
     1921 tagcacaact tgataaaata ttattagatt tattcaatca acaaaatgat agaggagcag
     1981 atgctcaatt taaagggcaa caataccaag tttatttcaa accaggtgat tatactgaaa
     2041 ctacatgtat gtatttaggg ttctatacat catttaatgg tttaggaaaa ataccaatgg
     2101 atgttaagtt aaataatatc gcaattccat cttatctaga tggtaataat gctacttgta
     2161 atttctggcg ttctgcagag aatctagctg ttataaatac tgggaatgaa caaggaaagg
     2221 ctggcttcga ttgtaatctt gcccgaccag aatattttaa ctgggcagta gctcaagctg
     2281 caccgcttcg tagag
//
LOCUS       DAOMTA010000510         2294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2210_length_2294_cov_2.099151, whole genome shotgun sequence.
ACCESSION   DAOMTA010000510 DAOMTA010000000
VERSION     DAOMTA010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2210_length_2294_cov_2.099151"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..819)
                     /locus_tag="PLK68_12185"
     CDS             complement(286..819)
                     /locus_tag="PLK68_12185"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRM91203.1"
                     /translation="MQYISKYTSPLGAITLASNGEALTGLWFDGQKYFGANLSKEYKN
                     VELPVFKQTKEWLNLYFNGQKPDFIPLLALQASEFRLAVWQILLEIPYGQTLSYGDIS
                     AVLAKQKGLKTMSAQAVGGAVGHNPISIIIPCHRVVGSNGNLTGYAGGLEKKIALLQL
                     EGADMGALFTPKKGTAL"
     gene            complement(962..1138)
                     /locus_tag="PLK68_12190"
     CDS             complement(962..1138)
                     /locus_tag="PLK68_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91204.1"
                     /translation="MKIEFKMSYKLGLLEFKVGDQLEISKNTIFESINDKYYMLILKG
                     LRYDLLKEDIKIIQ"
     gene            complement(1488..1757)
                     /locus_tag="PLK68_12195"
     CDS             complement(1488..1757)
                     /locus_tag="PLK68_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91205.1"
                     /translation="MNAFILLIPFLLIAFINEGAIKRAGYFAPLQGNEKIAYVIYQMA
                     NIGIFIYFFLSVIIDLSWQFCLGLLVYLLGLGMCIVLVKDFLSKK"
     gene            complement(1778..1954)
                     /locus_tag="PLK68_12200"
                     /pseudo
     CDS             complement(1778..1954)
                     /locus_tag="PLK68_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390711.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
     gene            complement(2096..>2294)
                     /locus_tag="PLK68_12205"
     CDS             complement(2096..>2294)
                     /locus_tag="PLK68_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM91206.1"
                     /translation="GCSTEFLDNLIQAEPKKIIYISCDVATQARDIKYLQEFGYHADV
                     CQPVDMFPHTTHIENIVRLSK"
BASE COUNT          811 a          394 c          258 g          831 t
ORIGIN      
        1 ctttgttcat aaactacatt ccttctttcc tataatattt aaatatatta tactatattc
       61 atttatttgt tacaatttat tgcataaatt atatatttct tacatggtaa ctcaaggtta
      121 ttatattcaa atctaaaaaa tacaatcaaa attaataaaa cagtaatctc caatccctat
      181 attggtaccc ttgactataa taaaatggag attttataac ttcatcttta ataagattca
      241 caaaatcatt cataataaat gcctccattt ttaagtttaa ttaacttata atgctgtccc
      301 ttttttagga gtaaacagcg ctcccatatc cgctccttct aattgaagca gtgcaatctt
      361 tttttcaaga ccaccagcat aaccagttaa gtttccgtta cttccaacga ctcgatgaca
      421 aggaataatg attgagatcg gattgtgtcc aactgcaccg ccaacagcct gtgctgacat
      481 tgtttttaaa cctttttgct ttgctaaaac ggctgaaatg tcgccatagc tgagggtttg
      541 tccatatggt atttctagca atatttgcca tacagcaaga cgaaattcac ttgcttgaag
      601 agccaaaaga ggaataaaat ctggcttttg accattaaaa tataagttaa gccattcctt
      661 tgtttgttta aatactggta actctacgtt cttatattct tttgataaat tagcgccaaa
      721 atatttttga ccatcaaacc atagacctgt taatgcttca ccattactag ctaatgttat
      781 tgctcctaac ggtgaagtat atttagatat gtattgcata tttcctcctt ataaataata
      841 attgttaaat attaaatgtt tattagtaat atttattata gcacagataa taaaattatt
      901 ttaagtttat tttccataac aaaaaatggc taatcactaa gatatagcca aattaaatat
      961 tttattgaat aattttaata tcttctttaa gaagatcata acgcaatcct tttaaaatta
     1021 acatataata cttatcattt atactttcaa atatagtgtt cttactaatt tctagttgat
     1081 cgccaacttt aaattctaat aatcctaatt tataagacat tttaaactct attttcattt
     1141 tcgcacctct atactggaac tcagtatacc attaatatta cttcaaaaca acaataagac
     1201 atcataagta tgctactaat atttattatt gttcttttta taattcttta tttaactata
     1261 tatatcttct tacttgattc atatattgtt cataagcaat tccaaacttg ctgatacacc
     1321 attgctcctc tgaaagaata acccaatgta tagagatttg aaatagtaat aaaattataa
     1381 ataagattat tgattgtgtt aataatacgg ttcttaaaaa gaaaataaaa tatgacatat
     1441 acattggatt tcgagagaaa cggtaaatct cgttagtatt taagccatca tttcttggat
     1501 aaaaaatctt taaccaaaac aatgcacatt cccaatccta acaaataaac cagtagacct
     1561 aaacaaaact gccaagataa atcaattatt actgataaga aaaaatatat aaatattccg
     1621 atattggcca tctgataaat tacataagct attttctcat tgccttgcag tggagcaaaa
     1681 tatccagccc gtttgattgc accttcgtta ataaatgcta ttaataaaaa aggaataagt
     1741 aaaataaatg cattcatttt gtttccccta tttctaatct aactacccct ataattggaa
     1801 acatgatcat cattacctct tagagcaacc atatgtacaa gtgtatttat caccaatttc
     1861 ttcaccatta cagcaataat gttctgcttt attcccacgt aaaaactgtt acttcaattg
     1921 taaattcata ttcttcatta taccattttt tcacaatcag tcgcccgctt ttattaggtt
     1981 atctaatttt actataatta tagatttaga acaattgaaa tcaatttact cgtcataaat
     2041 tataaaaaaa cagacttctt ttaggaaatc tgtttagtaa ttatttttat catggctatt
     2101 ttgacaaacg aactatattt tctatatgtg ttgtgtgagg aaacatatca acaggctgac
     2161 agacatcagc atgataccca aattcttgaa gatacttgat atcacgtgct tgagtagcta
     2221 catcacaaga aatataaatt attttcttgg gttctgcttg aatcaaatta tccaaaaatt
     2281 ctgttgaaca cccc
//
LOCUS       DAOMTA010000511         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2213_length_2291_cov_2.624776, whole genome shotgun sequence.
ACCESSION   DAOMTA010000511 DAOMTA010000000
VERSION     DAOMTA010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_2291_cov_2.624776"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLK68_12210"
     CDS             <1..901
                     /locus_tag="PLK68_12210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM91207.1"
                     /translation="LAIIYAFLVFFWVKDKVLKVQDDYDKLLTATKELSKGNFDVEID
                     GDLGIFNALNDEFKNIRIGFETAVKEETKSQNMKNELVSNVSHDLKTPLTCIKNYIVL
                     LQDDNLPIETRHEYLDNLNQYSNRLTNLIEDLFEVSKVNSGNIKLNLMELNIVALIEQ
                     AHAECSEILDAHQLTVITNYPHNDIILKLDGDKTYRIFENLFTNISKYALFNSRVYVD
                     LTEETEDITIIFKNISQEQMNFSPQEITERFVRGDKSRHESGSGLGLAIAKSFVEAQG
                     GTFNIAIDGDLFKAIITFKKIAK"
     gene            956..1639
                     /locus_tag="PLK68_12215"
     CDS             956..1639
                     /locus_tag="PLK68_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91208.1"
                     /translation="MKILFVGNSHTYMNDMPEMVRINSSEKLEVTMLARPAITFHDHL
                     ESMELQFALKQGYDFVIFQQAAHEPCPSKEATLHDAKALIELARSCGVMPYIMIPWSQ
                     RNYDDDFKTTKDIYHQVMMDNLVDGIPVGYVINRLSHQNPELELFQSDNQHLTSLGSY
                     LESITILNTIFFETKFPGKLIYPNQSSFEEHQLDERLIDFLTKEVVHTVERFKSNYCV
                     CGKREILDD"
     gene            1632..2066
                     /locus_tag="PLK68_12220"
     CDS             1632..2066
                     /locus_tag="PLK68_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91209.1"
                     /translation="MIRSVTKNDYEEIAKLMVEAFKNPPWNEVWSYERSYQRIEQLDD
                     GKYTRCFVYMLDNKIAGVVCGKLITYVNDLDFMIEDFYIDPHCQRRGLGKKMMKALEE
                     CLPEVDNLTLLTGREFYSADFYQKNGFKINDDMVFMVKKLKS"
     gene            2149..2274
                     /locus_tag="PLK68_12225"
     CDS             2149..2274
                     /locus_tag="PLK68_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91210.1"
                     /translation="MLNKFKGLKLKWYHFVVFWLPGGMLLLTGLLAYQYRNSKIV"
BASE COUNT          818 a          245 c          445 g          783 t
ORIGIN      
        1 cctagctatt atttatgcat ttttagtatt tttttgggtc aaagataaag tgctgaaggt
       61 gcaagatgac tatgataaat tattgactgc aacaaaagaa ttaagcaaag gtaattttga
      121 tgtagagata gatggtgatt taggtatttt taatgcttta aatgatgaat ttaaaaatat
      181 tcgaattggt tttgaaactg ctgttaaaga agaaactaag tctcagaata tgaaaaatga
      241 attagtaagt aatgtttctc atgacttaaa aacaccattg acatgtatta aaaattatat
      301 tgttttattg caagatgata atttaccaat agagacaaga catgaatatt tagataattt
      361 aaatcagtat tctaatcgtt taacgaattt aattgaagat ttatttgaag ttagtaaagt
      421 taatagtggt aatatcaagt taaatttaat ggaacttaat attgttgcat taattgagca
      481 ggcgcatgct gaatgtagtg aaatattaga tgcgcatcaa ttaactgtta ttactaatta
      541 tccacataat gatataatcc tcaaattaga tggtgataag acgtatcgta tttttgagaa
      601 tctattcact aatatttcta aatatgctct atttaatagc cgtgtatatg ttgatttaac
      661 ggaggaaact gaggatatta ctattatttt taaaaatatt tcacaagagc agatgaattt
      721 tagtcctcaa gaaattacag aaagatttgt tcgtggtgat aaatcgcgtc atgaaagtgg
      781 cagtggttta ggattagcaa tagctaaaag ttttgttgaa gcacaggggg ggacttttaa
      841 tattgcaatc gatggggatc tatttaaagc aataattact tttaaaaaga ttgctaagta
      901 gtcgtaattg aagctatact aaaggagtag tcctttgaaa gtaggtggaa ttaggatgaa
      961 aatattattt gttgggaatt cgcatacgta tatgaatgat atgccagaaa tggtgcgaat
     1021 caatagtagt gaaaagttag aagtgacaat gttggcccgt cctgcaatta cctttcacga
     1081 ccatttagag tcaatggaat tacagttcgc tttaaaacaa ggatatgatt ttgttatttt
     1141 tcaacaggca gcacatgagc cttgtccaag taaagaagca acattgcatg atgctaaagc
     1201 attgattgaa ttggcacgta gctgtggtgt tatgccatac attatgattc cttggagcca
     1261 acggaattat gatgatgatt ttaaaacaac caaagatatt tatcatcaag taatgatgga
     1321 taatttagtt gatggaatac cagtgggtta tgtcattaat cgtttaagcc atcaaaatcc
     1381 cgaattagaa ttgttccaaa gtgacaatca gcatttaact tcattaggga gttatcttga
     1441 aagcattacg attttaaata caattttctt tgaaacaaag tttcctggta aactcattta
     1501 tcctaaccaa agtagttttg aagaacatca attggatgaa agacttattg attttttaac
     1561 taaagaagta gttcatactg tagaacgatt taagagtaat tattgtgttt gtggaaagcg
     1621 agagatttta gatgattaga agtgtcacta aaaatgacta tgaagagatt gctaaactga
     1681 tggttgaagc atttaaaaat cctccttgga atgaggtatg gagttatgaa agatcatacc
     1741 agcgaattga acagttagac gacggaaaat atacaaggtg ttttgtgtat atgttagata
     1801 acaaaatagc tggtgttgtt tgtgggaaat taataacata tgttaatgat cttgatttta
     1861 tgattgaaga tttttatatt gatcctcatt gtcaaagaag gggattagga aagaaaatga
     1921 tgaaggcatt agaggaatgt ttacctgaag ttgataattt aactttgttg acaggacgag
     1981 aattttacag tgctgatttt tatcagaaaa atggttttaa gattaatgat gacatggtat
     2041 ttatggtaaa aaaattaaag tcttaataaa tctaaattat gccgaattat gttgacaata
     2101 tgtgattatc tccttatatt atatgtagat tgataaaggg gatataagat gctgaataag
     2161 tttaagggat taaaattaaa gtggtatcat tttgtagtat tttggctacc aggtggaatg
     2221 ctattattaa caggtttatt agcatatcaa tatcgtaatt caaaaatagt atgattattt
     2281 agcttgtaca a
//
LOCUS       DAOMTA010000512         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2227_length_2285_cov_2.077130, whole genome shotgun sequence.
ACCESSION   DAOMTA010000512 DAOMTA010000000
VERSION     DAOMTA010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2227_length_2285_cov_2.077130"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PLK68_12230"
     CDS             complement(<1..1069)
                     /locus_tag="PLK68_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRM91211.1"
                     /translation="MKKKKRLLPIVMASLMAMSINVGPLKAADAPLITNPSFEHEIDK
                     NEVQLYKAVRTDEKAFDGKYSIKVGNPKPENPSEVPIWRYNYGKGSVNVIFHNVEPNT
                     TYKITTHYWNESGVKMSTGVLDIEGKHTTSPWQLASNIQTNQKVSTEWQTNEHTITTG
                     PRTNEIYGFAYMEWTGNDLGSGVFYIDDFKIEEKASINYDADFSEFPETVPAVQNFEK
                     NGNEIYKLNTAKQVFSTDEFSKAKTQYLADSMVKKGLIKDYTIKTVTDASQGEGIVLV
                     KQPITFELPAAVTTSKIDAYQIDIDQDKTVIHSDYIQGIQNGAMTILQAFAQRKSLPA
                     GSVQDYSDQVVRGLQVASGRRS"
     gene            1413..2195
                     /locus_tag="PLK68_12235"
     CDS             1413..2195
                     /locus_tag="PLK68_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91212.1"
                     /translation="MAGFIYKLQNIVNTSPDSDDTNINIARCILKNIDKIKKRTSLQD
                     FADICYTSQSSISRFSQYLGYANFNDFKADCIGIQEETDEMIIDTRATKKINCVDYAQ
                     AISESLVRMQETSIDQEIEQLCEYIHTAKRIFIFATHIPGDLACIMQRAILTTGKFIE
                     FYPRKEHQMEVAKQIRRDDLCIFLSLEGTLLMEKAITIPTIISLAKNVLITQNVHIKF
                     SEQFTQVIGLGGHDSESIGKYKLLFFIDCLLNRYYHKYIVNN"
BASE COUNT          760 a          340 c          413 g          772 t
ORIGIN      
        1 aggatcttct tccagaggcg acctgaagcc cacgaacaac ttgatcactg taatcttgaa
       61 cagaaccagc aggaaggctt tttctttgag cgaaggcctg caatatagtc attgcaccat
      121 tttgaatacc ttgaatgtag tcagaatgga tgacagtttt gtcttgatcg atatcgattt
      181 gataggcatc gattttgctt gttgttacag ctgcaggaag ttcaaaagtg attggctgtt
      241 taactaaaac aataccttca ccttgtgagg catcagtaac agttttgatc gtatagtctt
      301 tgatcagccc ttttttaacc atactgtcgg ctagatattg ggtcttagct ttactgaatt
      361 catcagttga aaaaacttgc ttagctgtat ttaatttata aatttcattt ccattttttt
      421 caaaattctg aacagcaggg acagtttctg ggaattcaga gaagtctgca tcatagttga
      481 tagaagcttt ctcttcaatt ttgaaatcat cgatatagaa aactccagaa cctaagtcat
      541 taccagtcca ttccatatag gcaaaaccgt aaatttcatt agttctagga ccagtagtga
      601 tagtatgttc attagtctgc cactcagtgc tgactttttg gttagtttgg atattagaag
      661 caagctgcca aggagacgta gtatgcttac cttcaatatc aagaactccg gtagacatct
      721 ttactccgga ttcattccag taatgggtag taatcttgta tgtagtgtta ggctcaacgt
      781 tatggaaaat aacattaaca ctacctttac cataattata acgccagata ggaacttcag
      841 aaggattttc aggtttagga ttcccaactt tgatagagta ttttccgtca aaagcttttt
      901 cgtcagttct gacagctttg tagagttgaa cttcattttt atcaatttcg tgttcaaaag
      961 acggattagt aattagtgga gcatcggcag ctttaagtgg accgacgttg atcgacatag
     1021 ccataagtga ggccatgaca atcggtaata atctcttttt ctttttcata tatctctttc
     1081 cctttcatga tatgataata aataaaatat agcagttatt tgcgataaca cctcctgatt
     1141 aatatgatga tttgtctata catttctgta tctaactaaa ttgtaaaggg catgaggaaa
     1201 tgttacaata tagaaaattg caaatgattt ttaatttttg aatagatttt caaaaataga
     1261 atatcttttc acttcttgaa aagtagtgtt ataataatgg catgggttag tgctgttata
     1321 gttttatgaa taagagggga acaataaaag ccaaagaatt ttgaagtagt acgggaaagg
     1381 aggatatcga ggagattcct cgataacaga caatggctgg ttttatttat aaactgcaaa
     1441 atattgtaaa cacatcacca gatagcgatg atacaaatat aaatatcgct agatgcattt
     1501 taaaaaatat tgataaaata aaaaaacgga cttcattgca agattttgct gatatttgtt
     1561 atacatcaca atcaagtatt agtcgttttt cgcagtattt aggttatgca aattttaatg
     1621 attttaaagc tgactgtatt gggattcaag aagaaacaga tgaaatgatc atagatactc
     1681 gagcgactaa aaaaattaat tgtgttgact atgcccaagc aatttctgaa agtttagtaa
     1741 gaatgcaaga aacatcaatt gatcaagaaa ttgaacagtt gtgtgagtat attcataccg
     1801 ctaaaagaat ttttattttt gcaactcata ttcctggcga tcttgcctgc attatgcaac
     1861 gtgcaatttt aacaacgggt aagtttattg aattttatcc tcgtaaagag catcaaatgg
     1921 aggttgctaa gcaaatccgt cgagatgatc tctgtatttt tttatcatta gaagggacat
     1981 tattaatgga aaaagcaatc actattccaa caattatttc attagctaaa aatgttctga
     2041 taactcaaaa tgttcatatt aaattttcag aacaatttac acaagtaatt ggattaggtg
     2101 ggcatgatag tgaaagtatt ggaaaatata agttattgtt ctttattgat tgtttgttaa
     2161 atcgttatta tcataagtac atagtaaata attaaagtag aagtacagtt atttggacaa
     2221 ataactgtac tttattttta aaggatattt ttaggtgcta aagaaaaaat atcttgagag
     2281 aaata
//
LOCUS       DAOMTA010000513         2282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2231_length_2282_cov_2.169286, whole genome shotgun sequence.
ACCESSION   DAOMTA010000513 DAOMTA010000000
VERSION     DAOMTA010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_2282_cov_2.169286"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLK68_12240"
     CDS             complement(<1..200)
                     /locus_tag="PLK68_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91213.1"
                     /translation="MDNTVIKNLIYNQLFAAANYDLIATIAPDDPTKTRILNFSADCK
                     NNANMLDRIYQEENTSSYHPIV"
     gene            complement(252..779)
                     /locus_tag="PLK68_12245"
     CDS             complement(252..779)
                     /locus_tag="PLK68_12245"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRM91214.1"
                     /translation="MDLRLRPYQLSDQSALAALANNPQVSANLKDIFPYPYTPQDAQN
                     YLNFITQNSNNNLIEYAIIVDGLFAGAISITFGEDIYSHLAEIGYWLGEPYWHQGIMK
                     KAVKMIIKYIFDNYDTKIIKAEIFSRNIGSRKVLLANNFEYLVTLKKHAYKRGEFLDL
                     ELFELTRDKYQDNQL"
     assembly_gap    860..869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<870..1726)
                     /locus_tag="PLK68_12250"
     CDS             complement(<870..1726)
                     /locus_tag="PLK68_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM91215.1"
                     /translation="MEYYNLPQTNLKVSKVALGCMRIASKTPEEVENLVLESLKAGIN
                     FFDHADIYGGGKSEELFGKVLQKHPELRKEMIIQSKCGIRPGICFDFSKEYILASVDS
                     ILSRLQTDYLDILLLHRPDALMDPQEVSEAFDELYQAGKVRYFGVSNQNPGQIELLKK
                     YCKQPIIINQLQFGPAHAQMIDSGIYANMDEGIDHDGDILNYCRLNDITIQPWSTIRS
                     SLNEDTFIDNPKYPKLNEQLDKLADKYNVSKVAIVTAWILRHPAQMQPIAGTTSIKNM
                     LDTIKGVEV"
     gene            1811..1942
                     /locus_tag="PLK68_12255"
     CDS             1811..1942
                     /locus_tag="PLK68_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91216.1"
                     /translation="MNVKLKIFCGFLSGVIIGFIFSPVKHGVNVRVCNNGNHDRGEG"
     gene            1942..2142
                     /locus_tag="PLK68_12260"
     CDS             1942..2142
                     /locus_tag="PLK68_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HRM91217.1"
                     /translation="MSYQVIKMIVYVVSVMLSMWSLSCFNFDNVIRKAKVREFYLFFL
                     IASLCLGYLLGSFILEFTTIHF"
BASE COUNT          716 a          328 c          385 g          843 t
ORIGIN      
        1 tgaacaattg gatgatagct ggaagtattt tcttcttgat aaatacgatc gagcatattg
       61 gcattatttt tacaatcggc ggaaaaattc aaaatacgtg ttttagttgg atcatcggga
      121 gcgattgttg caatcagatc ataatttgct gcagcaaaaa gctgattata aattaaattt
      181 ttaattacgg tattatccat tttatcacct gttttaatat atgcacaaaa ataaaaaaga
      241 taaccaatct gttacaattg attatcttga tatttatcac gtgttaattc aaaaagctcg
      301 aggtctaaaa attcaccacg tttgtaagcg tgttttttta atgttacgag gtattcgaaa
      361 ttatttgcaa gcagaacttt tctactgccg atattacgac tgaaaatctc tgctttaata
      421 atcttagtat cgtaattatc aaatatatat ttaataatca tttttactgc ttttttcatt
      481 ataccttgat gccaataagg ctctccaagc caataaccaa tttcagctag gtggctataa
      541 atgtcttcac caaaagtaat agaaattgct cctgcgaata atccgtcgac aataatagca
      601 tattcgatta ggttattatt actgttttga gtaataaagt ttaaataatt ttgagcgtct
      661 tgtggtgtat aaggataagg aaaaatatct tttaggtttg cagaaacttg agggttgttt
      721 gccaaagcgg ctaaggcaga ttgatcactt agctggtaag gtcttaatcg taaatccatt
      781 acggtaaagg tttatcctct gcagtataaa tagcatacca ttcttcacga gttaattgta
      841 tgttagaagc ggtgcatatn nnnnnnnnnt taacctcaac tcctttaata gtatcaagca
      901 tattttttat actagttgtt ccggcaattg gctgcatctg tgccgggtga cgcaagatcc
      961 aggctgttac aattgctacc tttgaaacgt tatacttatc agcaagttta tctagctgtt
     1021 cgtttagttt aggatattta gggttatcaa taaaagtatc ttcgtttaat gaagatcgta
     1081 tagttgacca tggctgaatc gtaatgtcat ttaatcggca gtagtttaaa atatcgccat
     1141 catggtcaat tccttcatcc atattggcat agataccgct atcgatcatt tgagcgtgtg
     1201 ccggaccaaa ttgtaattga ttaataatga ttggctgttt acaatatttt tttagtaact
     1261 cgatctgacc gggattttgg tttgaaacac caaaatatct tacttttcct gcttgatata
     1321 attcatcgaa agcttcacta acttcttgag ggtccattag ggcatctgga cgatgtagta
     1381 ataaaatatc taagtaatct gtctgcaatc ttgataagat actatcgaca cttgctaaaa
     1441 tatactcttt agaaaaatca aaacaaattc cagggcgaat cccgcatttt gattgaataa
     1501 tcatttcttt tcttaattca ggatgttttt gtaatacttt accgaataat tcttctgatt
     1561 ttccaccacc ataaatatca gcatgatcaa agaagttaat accagctttt aatgattcta
     1621 ataccaggtt ttcaacttct tcgggagttt ttgatgcgat gcgcatacaa ccaagcgcta
     1681 cttttgaaac ctttaaattg gtttgtggta aattataata ttccatttta tttcctcctt
     1741 atatagatac atttgattat atcatgattg taaaaatcat ggtatactat atccaaggtt
     1801 aggtgataat atgaatgtaa aattaaagat tttttgtggc tttttaagtg gtgtaattat
     1861 aggatttatc ttttcaccag ttaaacacgg tgtaaatgtc agagtttgta ataatggtaa
     1921 tcatgatcga ggtgaaggat aatgtcatat caagttatta aaatgattgt ttatgtagtt
     1981 tcggtgatgc tgtcaatgtg gtctttaagt tgttttaatt ttgataatgt aattcgtaaa
     2041 gcaaaggtaa gagaatttta tttattcttt ttaattgctt cattatgttt aggatattta
     2101 ttaggttctt ttattttaga atttacaacg attcattttt aacatatttt taaatctctt
     2161 ccatataatt atgtgaggta attatatgaa agagataagt gttaaattgg gagtcgtttt
     2221 tttaattgca gtaatctttt tatcaattaa tgttggggta aaaaaagatg aaacaatcaa
     2281 ag
//
LOCUS       DAOMTA010000514         2282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2233_length_2282_cov_1.913785, whole genome shotgun sequence.
ACCESSION   DAOMTA010000514 DAOMTA010000000
VERSION     DAOMTA010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_2282_cov_1.913785"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLK68_12265"
     CDS             <1..481
                     /locus_tag="PLK68_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM91218.1"
                     /translation="LIRSKVLCPILFLTARVDEDDKINGLLSGGDDYITKPFSLRELE
                     ARIVTNIKREERHQQKTEYRFMDEMPIDYSEKIVAIAGHRMEFTKIEYQIIEFLSMHP
                     GQVFDKERIYAQVCGYDAEGDSRTITELVRRIRKKIADYSEKEYIETVWGIGYRWKK"
     gene            469..1647
                     /locus_tag="PLK68_12270"
     CDS             469..1647
                     /locus_tag="PLK68_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM91219.1"
                     /translation="MEKIRNLSLKKTILLYFVISLTAAFLLSGFTVHFAGNMQNKIWE
                     KYIDYADYTDVFQQYGKKYEIEISRPNQSQMNRLDYHLSEMCDFMETYSVLIFSIVGS
                     VAAVFFFYKNKLKTPLQELKDASQMIADNELDFHVSYENKDEMGTLCKEFEMMRSDLA
                     DNNRKMWRMIDDEKALRNAIAHDIRSPLSILRGYQEMLLEFVSAESIKTEDVIDILQT
                     GMYQIDRIEHFTENMRKMSHLEQRELQCSEIELSELAKKIEAEAAMLSKKESKLCKVE
                     RVQEQNIVKVDEELVMEVTDNLLENAVRYAQKSIALQIKKKDGFLIISVEDDGIGFVD
                     TEEKVTEPFYHKNPQDDLKHFGLGMYISRIFCEKHGGNLKIYNARQGGAHVEALFKAE
                     "
     gene            1764..>2282
                     /locus_tag="PLK68_12275"
     CDS             1764..>2282
                     /locus_tag="PLK68_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91220.1"
                     /translation="MKTIIKRSILDYLKNPVLWIGLIIIVASMYQCLSSYLQIHYIKQ
                     NEQITQNDVALEDADVMDGYIPTSDDKERRREWEDTIKETLMDTSKNGFGFSRQEADH
                     VMKEIQNMDVKTASEFLESQYGYYNAIDAYEDLEIHKGTAEEINHYIERKLSEHSFSW
                     YFAKKFTDFAGLH"
BASE COUNT          819 a          294 c          514 g          655 t
ORIGIN      
        1 tctgatacgc agtaaagtgt tatgtccaat tttatttctg acagcaagag ttgacgaaga
       61 tgataaaatt aatgggttgc tttcaggtgg ggatgattat attacgaaac catttagtct
      121 tcgggagttg gaagcaagga ttgtcacaaa cataaagaga gaagaaaggc atcaacaaaa
      181 aacagaatac cgcttcatgg atgaaatgcc gattgattat tctgaaaaaa tagtagctat
      241 agctggacac aggatggagt tcacaaaaat tgaatatcag attattgaat tcttatccat
      301 gcatccaggg caggtgtttg ataaagaacg tatatatgca caagtctgtg gatatgatgc
      361 ggaaggggac agtagaacga taacggaatt agtacggcgt ataaggaaaa aaatagcgga
      421 ttattcagaa aaagaataca tagaaactgt atgggggatt ggataccgat ggaaaaaata
      481 agaaacttgt cactgaaaaa aacaattctg ctttattttg tgatcagctt aacggcagca
      541 tttctgttat ctggatttac agttcatttt gcaggaaata tgcagaataa aatatgggaa
      601 aaatatattg attatgcaga ttatacggac gtgtttcagc aatatggaaa gaaatacgag
      661 attgagatat caagacctaa tcaatcgcag atgaatcgtt tggactatca tctttcggaa
      721 atgtgtgact ttatggaaac atattcggta ttgatttttt cgattgttgg gagtgttgct
      781 gcggtattct ttttttataa aaataagtta aagacacctc tacaggaact aaaagatgcc
      841 tcacagatga ttgcagataa tgaactggat tttcatgtgt cctatgaaaa taaagatgag
      901 atgggaactt tgtgtaaaga atttgaaatg atgcgaagtg atttagcaga taacaacaga
      961 aagatgtggc gaatgattga tgacgagaag gctttgcgga atgcaattgc acatgatata
     1021 cgttctccac tttccatatt gcgtgggtat caggaaatgc tcttagagtt tgtttctgcc
     1081 gagagtataa aaacagagga tgttattgat atcttacaaa caggcatgta tcagattgac
     1141 cgtatagagc atttcacgga aaacatgaga aaaatgtccc atttagaaca gagggaactg
     1201 caatgctcag agatagaatt atcggaactg gcaaaaaaga ttgaggcaga agctgccatg
     1261 ctgtccaaga aggaaagtaa attatgtaag gtagaaagag tgcaggaaca aaacattgtg
     1321 aaagtggatg aggaacttgt catggaagtg acagacaact tactggaaaa tgcggttcga
     1381 tatgcacaaa aaagtattgc tttgcagata aagaaaaagg atggttttct tataatttct
     1441 gttgaagatg atggaatagg atttgtggat actgaggaaa aagtaacaga gccattttat
     1501 cataagaatc cacaagatga tttaaagcat tttggccttg gtatgtatat ttctcgtatt
     1561 ttctgtgaaa agcatggagg aaatttgaaa atatataatg ccagacaagg cggtgctcat
     1621 gtagaagctc ttttcaaagc tgaataaaaa tactttataa ggctgaaatc acaaataaag
     1681 tggttttagt cttttttgtt gtgaatttat tgagaattga tttgtatgat gttgttaaag
     1741 aaataaagaa aggaagagat acaatgaaaa caatcataaa aagaagtata cttgattatt
     1801 taaagaaccc tgttttgtgg attggcttga ttattatagt tgccagtatg tatcaatgtc
     1861 tgtcatcgta tttgcaaatt cattatatca aacagaatga acagatcaca caaaatgacg
     1921 tagcattgga agatgctgat gtcatggatg ggtacattcc cacatctgat gataaagaaa
     1981 gaagaaggga gtgggaagat acgatcaaag agacgttaat ggacacctcc aaaaatggtt
     2041 ttggatttag caggcaggaa gcagatcatg taatgaaaga aattcagaat atggatgtaa
     2101 agactgcttc tgagtttttg gaatcccaat atggctatta taatgcaata gatgcttatg
     2161 aggaccttga aattcataag gggacagcag aagaaatcaa tcattatatc gagcggaaat
     2221 tatccgaaca ttctttttct tggtattttg ccaaaaagtt cacggatttt gcaggattgc
     2281 at
//
LOCUS       DAOMTA010000515         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2240_length_2279_cov_1.780126, whole genome shotgun sequence.
ACCESSION   DAOMTA010000515 DAOMTA010000000
VERSION     DAOMTA010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2240_length_2279_cov_1.780126"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLK68_12280"
     CDS             complement(<1..895)
                     /locus_tag="PLK68_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM91221.1"
                     /translation="MAEKKQFKAESKRLLDLMINSIYTHKEIFLRELISNASDASDKL
                     YYKALTENISSINRNDLKIMIEIDKENRVLTIKDNGIGMDKDELETHLGTIANSGSFE
                     FKNENEHDDIDIIGQFGVGFYAAFMVAKKVEVISKKYGTEQGYTWVSEVSDGYEIFET
                     NVADHGTTIKLYLKDNTDDDNYDEYLNQYHIRDLVKKYSDYVHYPINMDMTVSKKKDD
                     SDEYEDVIENQTLNRMVPLWKRNKKDITDEEYNDFYKDKFNDFNDPLKVIHSRVEGNV
                     SYDSLLFIPSKPPMNYYSNEYE"
     gene            complement(1034..1234)
                     /locus_tag="PLK68_12285"
     CDS             complement(1034..1234)
                     /locus_tag="PLK68_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRM91222.1"
                     /translation="MNTGTVKWFNSEKGFGFITKDTGGDLFVHFSAIQGSGFKSLEEG
                     AKVSFDIVESDRGEQAANVAAL"
     gene            complement(1430..2263)
                     /locus_tag="PLK68_12290"
     CDS             complement(1430..2263)
                     /locus_tag="PLK68_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM91223.1"
                     /translation="MEKKAIFLDVDGTLVANHGKMTEQVKKAITLARNQGHKVFICTG
                     RNKIGIRNELEEVNFDGFIASAGSYIEVADEVVHSRYYDRKLIEKACDVFNRNNIVYN
                     YECTDITYMSPKMMEFFVGGNNEEATNSEMERLKAEQSDKFNVRDMNEYDGRGIHKIS
                     FIAMKQEDFDHAFKELKENFNFVVHDMFGSKNINGEIMSKLDNKGTGIKRIVDYLNID
                     MEDTIGFGDSMNDYEMIDAVKCGVVMDNGSARLKEIADRICKSVDEDGVYYEFIDLGI
                     I"
BASE COUNT          749 a          442 c          224 g          864 t
ORIGIN      
        1 tttcatattc atttgaataa taattcattg gtggttttga aggaataaat aataatgaat
       61 catatgaaac gttaccttct actcggctat gaatcacttt caacggatca ttaaaatcat
      121 taaatttatc tttgtagaaa tcattatatt cttcatcagt aatatctttt ttattacgtt
      181 tccataatgg taccattcga tttaatgttt gattttcaat aacatcttcg tattcatcac
      241 tatcatcttt tttctttgaa actgtcatat ccatattaat tggataatgc acgtaatcag
      301 aatatttttt aactaaatca cgaatatgat attgatttaa atattcatca tagttatcat
      361 catccgtatt atcttttaag tataatttaa ttgtagttcc atgatcagca acattagttt
      421 caaaaatctc atagccatca ctaacttcag atacccaagt atatccttgc tcagttccat
      481 attttttaga aatcacttct actttttttg ccaccatgaa agctgcatag aaaccaacac
      541 caaattgtcc gataatatca atatcatcat gttcattttc atttttaaac tcaaatgaac
      601 cactattagc aatagttcca agatgtgttt ctaattcatc tttatccatc ccaattccat
      661 tatctttaat agttaaaaca cgattttctt tatcgatttc aatcataatt tttaaatcat
      721 tgcgattaat tgaagaaata ttttctgtaa gtgctttata atataattta tcacttgcat
      781 cacttgcatt agaaatcaat tcacgtaaga aaatttcttt atgtgtataa atagaattaa
      841 tcattaaatc taataatctt tttgattctg ctttaaattg ttttttctca gccatactca
      901 catctccatt ctaaatatta gcactcctca ttcatttgtg ctaaagatat tctaatacaa
      961 ctatttagca gtgtcaatag gaaagtgcta aattagtcaa aaataaaaag tctctattga
     1021 gacttttggt taattataaa gctgcaacgt ttgctgcttg ttcaccacga tcagattcaa
     1081 cgatatcgaa gcttacttta gcaccctctt ctaaagattt gaatccacta ccttggattg
     1141 cagaaaagtg aacaaataaa tctccacctg tatcttttgt aataaatcca aaaccttttt
     1201 cactattaaa ccattttact gtacctgtat tcatcagacg tacctcctat ttttattata
     1261 aaaagcatta ctataaaaaa agcagatgct aagttgctta taaatacaac tttcacatct
     1321 gtaagttaaa tattcattgc tcgttactca tatataatat cacagcaaca ataagaagtc
     1381 aaccaatatc agaataatat ttcaatttat attttataat ctttttaact taaattattc
     1441 ctaaatcaat aaattcataa tagactccgt cttcatcaac acttttacaa attcgatcag
     1501 ctatttcctt aagccgagca ctgccattgt ccatgaccac tccacatttg actgcatcga
     1561 tcatttcata atcattcata ctatcaccaa atccaattgt gtcttccata tctatattta
     1621 gataatcaac aattcgtttg attcctgttc ctttattatc aagttttgac attatttcac
     1681 cattaatatt tttactgcca aacatatcat gtactacaaa gttaaagttc tctttcaatt
     1741 ctttaaaagc atgatcgaaa tcttcctgct tcattgcaat aaaggatatt ttatgaattc
     1801 cacgaccatc gtattcattc atatctctta cattaaattt atcactttgt tcagctttta
     1861 agcgttccat ctccgaatta gtcgcttcct cattatttcc acctacaaaa aattccatca
     1921 ttttgggtga catatatgta atatcggtac actcataatt atatacgata ttattacgat
     1981 taaatacatc gcaagctttt tcaattaatt tacgatcata ataacggcta tggactactt
     2041 catcagcaac ttcgatatag cttccagcac tagcaataaa accatcaaaa tttacttcct
     2101 ctaactcatt tctaattcct atcttatttc ttcctgtaca aataaatact ttatgtccct
     2161 gattacgggc tagagtgatt gcttttttta cctgctcagt cattttgcca tgattagcaa
     2221 ccaatgttcc atcaacatct aaaaaaattg cttttttctc cataacattc ctccttaag
//
LOCUS       DAOMTA010000516         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2242_length_2278_cov_2.252362, whole genome shotgun sequence.
ACCESSION   DAOMTA010000516 DAOMTA010000000
VERSION     DAOMTA010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2242_length_2278_cov_2.252362"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLK68_12295"
     CDS             complement(<1..444)
                     /locus_tag="PLK68_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91224.1"
                     /translation="MKILYISRKSFSDSDFPLLRELQETGIEVDAYFLGDLKSSGILE
                     FDKKSHFWGIKRASEFNEVSKFYGQYVNLRNFFFVSFPNGRKGEIIRLFEFFYLYYHL
                     KRIDYDVIHLIWPLNGFSERLLYKLKPKILCTVHDPIPHSGQEWQE"
     gene            complement(986..2053)
                     /locus_tag="PLK68_12300"
     CDS             complement(986..2053)
                     /locus_tag="PLK68_12300"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HRM91225.1"
                     /translation="MNFTNFLELKIRQNHVLFLLTRLVKRLILFIPRSYDIILNEQKN
                     KQILGFRQWSKLVFYKIILILAEGKLRHDINERKHNIIRKFISTNYKVISTEDVPSIT
                     LNNHENCIWVFWWQGQNHFPAIIRTCYESIIQNSGGKKVILITKENFQIYVDIPDALI
                     NRVNEDKMLIAHLSDYVRFKLLYKYGGVWIDSTFFVTQNIGSNNEFNYDFYSIHYEHP
                     LDDNCVALCRWVSSLLFAKAGNGIMYNIYIILEKYMLKYDNLIDYLFIDYLLDFLASN
                     SETYNEVINNIPFNNENSMVLQKKLPYIYNEKDYCNIINNGTSYFKLTYKTKAKEFIG
                     NKQTYYGYLVNQYNTDIKPYK"
BASE COUNT          754 a          339 c          291 g          894 t
ORIGIN      
        1 ttcttgccat tcttgaccag aatgaggaat gggatcatgt acagtacaca atatttttgg
       61 ttttaactta tacaaaagcc tttcggagaa accatttaat ggccatatta aatgtatgac
      121 atcataatca attcttttta agtgataata caaatagaaa aattcaaata atcttataat
      181 ttcacctttc cttccattag gaaatgatac aaagaaaaaa tttcttaaat ttacatattg
      241 tccataaaat ttcgatactt cattaaactc agatgctctt ttaatacccc agaaatgact
      301 ttttttgtcg aattctaaaa tcccactaga tttcaggtca ccaaggaaat atgcatcaac
      361 ttcaatgcca gtttcctgta attctctaag tagaggaaag tcactatctg aaaatgactt
      421 tctggagata tatagtattt tcatatattt tttttatttc cactttttca aaagtggttg
      481 ttattatgca attttgttgt aaacgttcta ttttccgctc catagttctt tatcggcttt
      541 gtcaagcata taagagataa cgtgaggata taaaactttt tttgatttaa cattattggt
      601 tgtagtaaca atgtaaaagt tgtaccttgt cgacttcctt tccatctttg ataatggtga
      661 ggtgttcagt cagataattc tcggaattaa tatcgttgag tgtacagctt tttgttacag
      721 agaatattac tgcagcattc tcagcagcgt cttcgcttcc gatattctgg cagtttttga
      781 gatacaactt cactactttc attctctatt cacataaaat atttgatatt tcaaccattc
      841 tttccctgat ggaatattac tggttcaagg tgtagtcgac gacctttctc atcatcttat
      901 tttccataag tgaggtaaac ggtacgtcag ttttgaccat ttactcatgg atgtttataa
      961 taagataatt ttaataaaac tgtttctatt tataaggttt gatgtctgta ttatattgat
     1021 ttaccagata accataatat gtctgtttgt ttcctatgaa ctcttttgct ttggttttat
     1081 aggttaactt aaaataacta gtaccattat ttataatatt acaataatct ttttcattat
     1141 aaatgtaagg aagtttcttt tgtagtacca ttgagttttc attattaaac ggaatgttgt
     1201 ttattacttc attataggtt tcactattac ttgctaaaaa atctaaaaga taatcaataa
     1261 aaagataatc aattaaatta tcatatttta gcatatattt ctcaagaatt atataaatat
     1321 tgtacattat accatttcca gctttagcaa ataataaaga ggaaacccaa cgacataaag
     1381 ccacacaatt atcatctaaa gggtgttcgt aatgaataga atagaaatca taattgaact
     1441 cattattact accaatattt tgagttacaa aaaatgttga atctatccat actcccccat
     1501 atttatacaa taatttaaac cggacataat ctgacaaatg agcaattaac attttatctt
     1561 cattaactcg attaataagt gcatctggaa tatcaacgta tatttgaaaa ttttcttttg
     1621 ttattaatat aacctttttc cctccagaat tctgtataat tgattcatag caagttctaa
     1681 ttattgcagg aaaatgattt tgaccttgcc accagaatac ccagatacag ttttcgtgat
     1741 tatttaacgt tattgaaggt acatcctctg tcgatatcac tttgtaattt gttgatatga
     1801 attttcttat tatattatgt ttcctttcat ttatatcatg tcgcaattta ccttcagcca
     1861 gtattaaaat aattttataa aagactaatt tcgaccattg tctgaaacct agaatttgtt
     1921 tatttttttg ttcatttagg ataatatcat atgatctagg aataaataaa attaatcttt
     1981 taactaatcg tgttaatagg aacagaacat gattttgacg gattttaagt tcaagaaaat
     2041 ttgtaaaatt cataaatcgt atgaatctat aattttaaca tctttttata ttgtgctatc
     2101 gcaaaacatt tcaacaagaa agttatactt aacttgtatt cttttcttct ttttaaaata
     2161 ttaaagagat atgtaagatt attcttgaaa agtgttatta gaatatttct tttcttactt
     2221 atgaaatgcg ttttttttat cttgttgaga tattttaaat aaagtttatc aacttcat
//
LOCUS       DAOMTA010000517         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2244_length_2277_cov_2.808281, whole genome shotgun sequence.
ACCESSION   DAOMTA010000517 DAOMTA010000000
VERSION     DAOMTA010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2244_length_2277_cov_2.808281"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLK68_12305"
     CDS             <1..887
                     /locus_tag="PLK68_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM91226.1"
                     /translation="AFCEWSKECENRFIKLKDNEEHLNKIFIAIYNLQNEMSFNVDDD
                     AVTVRKADLNRDIKSFISYAIGCMFGRYSLNVEGLVYAGGEWDNSKYSLFTPDKDNII
                     PICDDEYFEDDIVSRFVEFVKAVYGEDTLEENLKYIASSLGGKGTPREVLRNYFLNDF
                     FKDHCNTYQVTGSGKRPIYWLFDSGKKNGFKALVYIHRYTPDLIARMRTQYIHEQQAR
                     YRNQIEMLERQIDGDVSTSERVRLNKQLKKFKEQDEELRKYEEKIHHWADRMEPMDLD
                     DGVKANYAKFQELLAKIK"
     gene            904..>2277
                     /locus_tag="PLK68_12310"
     CDS             904..>2277
                     /locus_tag="PLK68_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HRM91227.1"
                     /translation="MELESIIKQLNQKFAEPLPEFYERRIIVWNDEDQSFIDKLDDFE
                     LDNAKLLILNEKNNFEVKRILSHDDLTSNYLLYNPFNTDMEDDWLLDIKLFSDEFRAD
                     QLSMWMQEMNIISTPALRKSIKIYQGFLNAISRRKLVSQFGEEIDKPSKLHLAILASI
                     CKFRTLNPKDIIKAVMADGYDLKNGLKMSLLQYNASDTFWALVNKATGFDGIKQGNID
                     DLDIHIILSAMHKTMDEKVLSGLDNRYSPVHDGFCYEMIYEWLHSDKKDDLIDVLRNV
                     ERSLKLVDRFNQFELDDLVDTEILPCIDEVILNKLMQQIIQHTISADQIIAICEKRRT
                     MVWYKDNENYYRGLYQAAMMQKFYDRNIAGFHNTEAYKMWKVYTEEYYLMDTYYREFH
                     VAFDNTLKASNPYLDDNFKSVADEMEKLYKNWYLDKLAENWTNIAEAELSQKGEIARV
                     NQQVEFYN"
BASE COUNT          866 a          250 c          424 g          737 t
ORIGIN      
        1 atgctttttg tgaatggtct aaagagtgtg aaaatagatt tattaagtta aaagataatg
       61 aggaacatct taataaaatc tttatagcaa tttataacct tcaaaatgaa atgtctttta
      121 atgtagatga tgatgcagta acagttagaa aagctgattt aaatagagat atcaaatcat
      181 tcatcagtta tgctattgga tgtatgtttg gtagatattc attaaatgtc gaaggtttag
      241 tttacgcagg cggtgaatgg gataacagta aatactcatt atttactcca gataaagata
      301 atatcattcc aatttgtgat gatgaatact ttgaagatga tattgttagc agattcgtag
      361 agttcgttaa agcagtatat ggtgaagata cattagaaga gaacttaaag tatattgcaa
      421 gttctttagg tggtaaaggg acaccgagag aagttttaag aaattacttc ttaaatgatt
      481 tctttaaaga tcattgtaat acatatcaag tgactggctc ggggaaacgt cctatttact
      541 ggctatttga tagtggtaaa aagaatggat ttaaagcatt agtatatatt catagatata
      601 caccagattt aattgctcgt atgagaacac agtatattca cgaacaacaa gcacgttatc
      661 gcaatcaaat tgaaatgtta gaaagacaaa ttgatgggga tgttagtact tcagaacgtg
      721 tacgtttaaa taaacaatta aagaaattta aagaacaaga cgaagaattg cgtaaatatg
      781 aagaaaagat tcatcattgg gctgatagaa tggaacctat ggatttagat gatggtgtaa
      841 aagctaatta tgctaaattc caagagttac tagcaaaaat aaaataggaa ggaggttctg
      901 aaaatggaac ttgaaagtat aattaaacaa ttaaatcaaa agtttgcaga acctcttcct
      961 gagttctatg aacgtagaat tattgtatgg aatgatgaag atcaaagttt tattgacaag
     1021 ttagatgatt ttgaattaga taatgcaaag ttattgattc taaatgaaaa gaataatttt
     1081 gaagtgaaac gaatcttatc tcatgatgat ttaacgtcta actatcttct ttacaatccg
     1141 tttaatacgg atatggaaga tgattggtta ttagatatta aattatttag tgatgaattt
     1201 agagcagatc agctttctat gtggatgcag gaaatgaata ttatttcaac tcctgcttta
     1261 cgtaaatcta ttaagatata tcaaggattc ttgaatgcta ttagtagacg taaattagtt
     1321 tcacaatttg gtgaagaaat tgataaacca tcaaaattac atttggctat attagcttct
     1381 atttgtaagt ttagaactct taatccaaaa gatatcatta aagcagttat ggcagatggc
     1441 tatgatttaa agaatggatt aaagatgagc ttattacaat ataatgcatc agatacattc
     1501 tgggcattag ttaataaggc tactggattt gatggaatca aacaaggaaa tattgatgat
     1561 ttagatattc atatcatttt aagtgcaatg cataagacta tggatgaaaa agtattatcg
     1621 ggattagata atagatattc tccagtgcat gatggatttt gttatgaaat gatttatgaa
     1681 tggcttcaca gtgataaaaa agatgactta attgatgtat taagaaatgt tgaaagatca
     1741 cttaaattag ttgatagatt taatcagttt gaactagatg atctagtaga tactgagatt
     1801 ctaccttgta ttgatgaagt catcttaaac aagttaatgc aacaaattat tcaacataca
     1861 atcagtgctg atcaaattat tgctatctgt gaaaagcgta gaacaatggt ttggtataag
     1921 gataatgaaa actattatag aggcttatat caagcagcaa tgatgcaaaa attctatgat
     1981 agaaatattg caggattcca taatacagaa gcatataaga tgtggaaggt atatacagaa
     2041 gaatactatt taatggatac ttactataga gaattccatg tggcctttga taacacatta
     2101 aaagcatcta atccatattt agatgataat ttcaagtctg tagctgacga aatggaaaag
     2161 ctatataaga actggtattt agataaatta gctgaaaatt ggacaaatat agctgaagct
     2221 gaattatcac aaaaaggtga aattgctcgt gttaatcaac aagtggaatt ctataat
//
LOCUS       DAOMTA010000518         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2245_length_2276_cov_2.248086, whole genome shotgun sequence.
ACCESSION   DAOMTA010000518 DAOMTA010000000
VERSION     DAOMTA010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_2276_cov_2.248086"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..423
                     /locus_tag="PLK68_12315"
     CDS             250..423
                     /locus_tag="PLK68_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91228.1"
                     /translation="MSVLYDPIIPKLDYMQLWFYNWLMLDKEYPIDKIVRLSNEEYKK
                     EIQLFVEMYQNIK"
     gene            656..1468
                     /locus_tag="PLK68_12320"
     CDS             656..1468
                     /locus_tag="PLK68_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91229.1"
                     /translation="MGMKDWAKREVEIASAKERGNMPADEFDYGCACYDSALKAFNSL
                     CEEYNHSGASIRFTKFILDRLIDGKPLTPIEDVPEVWNDIADVSGGGIKYQCNRMPSL
                     FKEVFPDGHIEYDDVDQCYCEDINAGSTYTCELESNIIQELYPITMPYIPGDRIKVIT
                     EECLAYKKNGDFDTKAIFCCIKDGEKIEINRFFAEAQDYPDRKEVSVGHGLVEITYEE
                     YVGRKVIAGALRKEIILKQCEMNCAAFENCSYKEEHSENYDWECDKEGSVNT"
     gene            1913..2269
                     /locus_tag="PLK68_12325"
     CDS             1913..2269
                     /locus_tag="PLK68_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91230.1"
                     /translation="MSESLKSVFAAVGLIALLTVLLCMVLMIICEVANIIEETRRQYR
                     IKHRFNKPPTAKCYCRDCKFWIPENGECHASHTAKGYYMADNWFCYSAEPVTGEIYKQ
                     REEQIRKNTEERIDKK"
BASE COUNT          796 a          271 c          505 g          704 t
ORIGIN      
        1 taaaattgtg attgtggttc tgttacttgt tattctgggc attgcagcag caatgatagc
       61 gatcagcaat atgtcctgcc attttgataa ggacaaacac taaggaggtt tatatggtta
      121 ctactagcga ttgtaatacc tgtagtcgaa aagatatata cagtgaaata agaaaggtat
      181 atacacagtt ttgtgatcaa tttttggatc ttgatccggt ttctgagaaa tagataagga
      241 gaagagaaaa tgagtgtttt gtatgatccg attatcccta aattagacta tatgcagtta
      301 tggttttaca attggttaat gcttgataag gagtatccaa ttgataaaat cgtaagactt
      361 tctaatgaag aatacaagaa agaaatacag ttgttcgtag agatgtatca aaacattaaa
      421 taaaggagaa ttggatattg tattaattgt tttggagttg cataggcgtt gatttattgt
      481 tgggtggaag aatctgccat tgtatagacg actccagttt attgtattat gcattttaat
      541 agggctgatc ccgtttgtta atgtggccct ttctattttt atatttatct ttataaaaga
      601 aattgttgat gatattacaa aataaaaata ggttctcttg aaaggagaaa aaaagatggg
      661 aatgaaagat tgggcaaaga gagaagttga aattgcatcc gcaaaagaaa gaggaaatat
      721 gcctgctgat gagtttgatt atggttgtgc ctgctatgac agtgctttaa aagctttcaa
      781 tagtctgtgt gaggagtata accacagtgg ggcaagtatt aggtttacta agtttattct
      841 ggatcgattg attgatggaa aaccgctgac accgatcgaa gatgttccgg aagtatggaa
      901 tgacattgct gatgtttcag gaggcgggat taaatatcaa tgcaatcgga tgccttcact
      961 ttttaaggag gtgtttcctg atgggcatat tgagtacgat gacgttgatc aatgctattg
     1021 tgaggatatt aatgcgggta gcacatatac ttgtgaatta gaaagtaata ttattcagga
     1081 gttatatcct attacgatgc catatattcc aggtgacaga atcaaagtaa ttacagaaga
     1141 atgtctggca tataagaaga acggagattt cgatactaaa gctattttct gttgtataaa
     1201 agatggagaa aaaatagaaa tcaatcgttt ctttgctgaa gcgcaagatt atccagatag
     1261 aaaagaagtc agtgttggtc atggcttggt tgaaattaca tatgaagaat atgttggacg
     1321 taaagtaatt gcaggggctt taagaaaaga aattatatta aaacagtgtg aaatgaattg
     1381 tgcagcattt gaaaattgtt cttacaaaga agaacatagt gaaaattatg attgggaatg
     1441 tgataaggaa ggtagtgtaa atacatgaac gatatatata aaccattaac accagctttt
     1501 agagctgata ttcagagttc tatagatgct aatattgcag agttacgaag gaaattgaca
     1561 aaacagcatc attaattgat aggactaagg atatgagtgg ctgtattata ttaagcataa
     1621 taccaatttt taatcttctt atggccggtt atatgtttgt tggaattgac accatatata
     1681 aaagtaaaag aaaaatgttt accggaagat cctgctactc gtttgtcgcg taatacattg
     1741 gaagcgtttg aaaaagctat gaaggattct gaggaatcta cgaaggagac gcatttatga
     1801 acaattatgg agatgtgtac ggacgattct tctgggcatc agacactggg gattgttcct
     1861 gttaataata ttaaatacat aaaaatgtta agttagaaga aaggaaaaaa gtatgagtga
     1921 atcattaaaa agcgtatttg ctgcggttgg attaatagca cttcttacag tattactttg
     1981 tatggtatta atgattattt gtgaagtagc aaatataatt gaagaaactc gaagacagta
     2041 cagaatcaag catcgtttta acaagccacc gacagcaaaa tgttattgca gagattgtaa
     2101 attctggatt ccagaaaatg gtgaatgtca tgcctcacat acagcgaaag gatattatat
     2161 ggctgataat tggttttgtt attctgctga gcctgtcaca ggtgaaatct ataaacagcg
     2221 ggaagagcag attaggaaaa atactgaaga aaggattgat aagaaatgaa taaaat
//
LOCUS       DAOMTA010000519         2267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2257_length_2267_cov_2.073689, whole genome shotgun sequence.
ACCESSION   DAOMTA010000519 DAOMTA010000000
VERSION     DAOMTA010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_2267_cov_2.073689"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLK68_12330"
     CDS             <1..782
                     /locus_tag="PLK68_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM91231.1"
                     /translation="FQQAYRIAIDKINEYPTCDNLIVNVAVVLEGLLSLNEMDEAIKY
                     QQKIDSLLEQCLTSDNPMIKNQAQAVLINKYINQGKLEQAEKLINELPEQHLIDKKQK
                     LVNLYIAQAKLEAAAKLQEEKLLVTVNEIPTMLLTLMEIAIKQERYDDAEIIADIYKE
                     MHEVFGLWKYSSYTAHFQLCINRKKRLECLKILKEMFNAINKGWNINTSPLYRHITAK
                     KIDQTFVQEMKNMLVTSIKSDPDCKFITDDLEMNKILEKYK"
     gene            987..1394
                     /locus_tag="PLK68_12335"
     CDS             987..1394
                     /locus_tag="PLK68_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HRM91232.1"
                     /translation="MTIETNDNLNQLIKAYNHDIELNRLKDILDYLKSKPIYMPSTIK
                     ISKQKLLKIPNDDLIMILDNLSGETTQNYLPVYSSPVLFNSKYRYFVTLSIYDCFMLM
                     KRFKEKNAIIINPNRDNSLVIDNILIKYLKNGG"
     gene            2086..>2267
                     /locus_tag="PLK68_12340"
     CDS             2086..>2267
                     /locus_tag="PLK68_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91233.1"
                     /translation="MNLVTTMLINVINNSHNSVVDCDIAKYLIANHENINSLSISELA
                     KNTNVSISQVSRFVRS"
BASE COUNT          908 a          248 c          353 g          758 t
ORIGIN      
        1 attttcagca ggcatataga attgcaatag ataaaataaa tgaatatcca acttgtgata
       61 atctaattgt taatgtagca gttgttttag aaggattgct ttcattaaac gagatggatg
      121 aagctataaa atatcagcaa aaaattgaca gtttattgga acagtgttta actagtgaca
      181 atccaatgat taaaaatcaa gctcaagcag ttttgatcaa caagtatatt aatcaaggga
      241 aacttgaaca ggctgagaag ctaattaatg aattaccaga acaacattta atagataaaa
      301 aacaaaagtt agtcaatctc tacatagctc aagcaaaatt agaagcagcc gctaaattac
      361 aagaagaaaa acttttagtg acagttaatg agattccaac tatgctttta actttaatgg
      421 agattgcaat taaacaagag cggtatgatg atgctgaaat aatcgccgat atatataaag
      481 aaatgcatga agtttttgga ttatggaaat attctagtta tactgcacat tttcaattat
      541 gtataaatcg taaaaaacgg ttagagtgtt taaaaatatt aaaagaaatg ttcaatgcaa
      601 ttaataaagg atggaatatc aatacttctc ctttatatcg ccatattaca gcaaaaaaga
      661 ttgatcagac ctttgttcaa gaaatgaaaa atatgcttgt gacatcaatt aaaagtgatc
      721 ctgattgtaa atttattacc gatgatcttg aaatgaataa aatcttagaa aaatataaat
      781 gatggttaaa tatcatcatt ttttataata tttaagaata tgcacaagta attcacaatt
      841 tgtgaattac tctgtgattg caaggtagtc gcgtatcatt tagtattaaa cataatgtat
      901 aagaaaaggt gaataacgcg tttctttata agcttagatg tgttacaata atatccttga
      961 aaatcaattt gaaagtcggt gagtatatga ctatagaaac taatgataat ttaaaccagc
     1021 taataaaagc atacaatcat gatattgaac tcaatcgttt aaaagatatt cttgattatt
     1081 taaaatcaaa accaatttat atgccttcga caattaagat ttcaaaacaa aaattactaa
     1141 agatccccaa tgatgatcta ataatgattt tagataattt atctggagaa actactcaaa
     1201 attatttacc tgtatattct agtcctgttt tattcaattc aaaatatcgc tattttgtta
     1261 ctttgagtat ttatgattgt tttatgttaa tgaaaagatt taaagagaaa aatgcaataa
     1321 ttattaatcc taatagggat aattcattag ttattgataa catattaatt aagtatttaa
     1381 aaaatggcgg ataaaaacta aatttatgtt tattgatcca tatcgcagct tgtaaataga
     1441 aatgatatgg atcatttaat tgttgccttt taaattgtat gataagataa ttttaatgat
     1501 aaataggtat ttatattaca tggaataaag aaatagaaag gttaaatgga aacgtaaatc
     1561 aaactattga aaagaaaggg gatagaatct atcgtaacag ctattggaaa ccttatatcc
     1621 atgatttttc acaatattta gaatttaaaa agtgtaatag agttccaaga tttttaggcg
     1681 atgatgaaaa taatcgtgag atattatctt ttttgcatga atatgtatct ggtaacaact
     1741 ttcttaattg tcatgcaatt gtttggacag attatattgt cacaatgatg ctagttatca
     1801 gactaattcg gatggaacaa taagagatta taagacaaat gatactttgt ttagaaaaga
     1861 aagaataaaa atatttttta aagcttacgg aatgattata ccagataatc tctttatttg
     1921 ggggatatgt tgaatcgaag aactttgtaa tttgactaat catgaggtat tggctggaaa
     1981 taaagctttt caaaaaataa tgagattaat tattatcaaa atgaaattac ttttataaaa
     2041 aacaatagta aagcctggta ttgaattatg aataggagaa attggatgaa tttggtgaca
     2101 acaatgttaa taaatgtaat aaataattct cacaatagtg tagttgattg tgatattgct
     2161 aaatatttaa tagcaaatca cgaaaatatt aatagtctat caattagtga gttagcgaaa
     2221 aatacaaatg tttcaatatc gcaagtaagc cgatttgtac gatcgtt
//
LOCUS       DAOMTA010000520         2266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2259_length_2266_cov_2.554048, whole genome shotgun sequence.
ACCESSION   DAOMTA010000520 DAOMTA010000000
VERSION     DAOMTA010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2259_length_2266_cov_2.554048"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLK68_12345"
     CDS             complement(<1..602)
                     /locus_tag="PLK68_12345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM91234.1"
                     /translation="MKHGEISASMMCSDLIDLKETLNVFEEEGVEDLHIDMMDGSFVP
                     NFGLGVDYIRGLRKLTSIPLDLHLMIKDPEYKLQWIGIKNTDIVSIHYESTYQVQRVL
                     DWLEPYKCKRFLAINPATPVSALEEVLDYIDGINLLMVNPGFAGQKIVPSTMNKAAKV
                     IDLLKRENHNEIEVEVDGNITPENGQKLRTLGASIFVCGTS"
     gene            complement(599..1129)
                     /locus_tag="PLK68_12350"
     CDS             complement(599..1129)
                     /locus_tag="PLK68_12350"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM91235.1"
                     /translation="MGTGMIGISAGQWAYKKGAASVTVIGRSEAKRTLVETCGLHYVN
                     SSVDDLKEYDFVIEAVGSQYTIQQAISQTRPEGTVLLMGNPFGDINLDQNVYWKILRK
                     QLLIKGTWNSKYNGKDFSDWTEAVKAIANNEINIKPLISHQYSEDKLFDGLKLMYNKQ
                     EPYCKVMTIWNKKTEI"
     gene            complement(1145..1627)
                     /locus_tag="PLK68_12355"
     CDS             complement(1145..1627)
                     /locus_tag="PLK68_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRM91236.1"
                     /translation="MKAYRLYAVNDLRYEDIEIPKIPCSDWCIVKVYAAGICSSDIPR
                     IFKKGTYHFPTIPGHEFSGEVFSVGENKYSNLVGKKVGVFPLIPCRKCSQCMEKKYEM
                     CANYDYVGSRRDGGFAEYVAVPIWNLIELPKNMPYTSAAMLEPLSVALHAVKTGGMKK
                     "
     gene            complement(1629..>2266)
                     /locus_tag="PLK68_12360"
     CDS             complement(1629..>2266)
                     /locus_tag="PLK68_12360"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HRM91237.1"
                     /translation="PKQYLKVKEKPIFYYCVNTFAKRKDIDAIIIVVADEWRNYVKKY
                     MSHIDKPIYYAHSGKTRQFSIYNALLATKKAGYSDDDIVIIHDAARPLVTNDIIDQCI
                     NFINEGYDGVLPVIHVKDTIYQSNNGSSITNLLNRNELFAGQAPESFKLGKYLNIHKD
                     ISHEEIAKINGSTEIAVKTGLKIKLAEGSEINFKITTPEDLTTFKQIMEIK"
BASE COUNT          693 a          426 c          325 g          822 t
ORIGIN      
        1 gaagttccac aaacaaatat actagcacct aatgttcgta atttttgacc attttctgga
       61 gtaatgtttc catcaacttc aacttctatt tcattatgat tttctctttt taataaatcg
      121 ataacctttg ctgctttatt catcgtacta gggactattt tttgtcctgc gaaaccagga
      181 ttaaccatta ataggttgat accatcaata taatctaata cttcttctaa agcagatact
      241 ggtgtcgcag gattaatagc aagaaatctt ttacacttat atggttccaa ccaatctaat
      301 actcgttgaa cttgataggt gctttcgtaa tgaatactaa caatatcagt atttttaata
      361 ccaatccatt gaagtttata ttctggatct tttatcatta ggtgtaaatc taatggaata
      421 cttgtaagtt tgcgtaatcc acgaatatag tctacgccaa gaccaaagtt cggaacaaaa
      481 gaaccgtcca tcatatcaat atgtagatct tcaacaccct cttcctcaaa tacattaaga
      541 gtctctttta aatcaattaa atctgagcac atcattgatg cagatatttc tccgtgtttc
      601 atatttctgt ttttttgttc caaatggtca ttactttaca atacggttct tgtttattat
      661 acataagttt caaaccatca aaaagtttat cttcagaata ttgatgagaa attaatggct
      721 ttatgttaat ctcattgttc gctattgctt ttactgcttc tgtccaatca gaaaaatcct
      781 ttccattata ttttgaattc caagttcctt ttattagaag ttgttttcgg agaattttcc
      841 aatacacatt ttggtctaag ttgatatctc caaatggatt acccatgagt aaaacagtac
      901 cttcaggtct tgtctgtgag atagcttgct gtattgtgta ttgcgaacct acagcttcta
      961 taacaaagtc atattctttt aaatcatcta cgctgctatt aacatagtga agtccacagg
     1021 tttctactaa tgtccttttg gcttcagaac gcccaatgac agtgactgat gcggctcctt
     1081 ttttatacgc ccactgtcct gcacttatac caatcattcc cgttcctata atggcaacat
     1141 gttctcattt tttcattcct ccagttttaa cagcgtgcaa ggcaacagat agaggttcaa
     1201 gcatagctgc tgaagtatat ggcatattct taggaagttc tattagattc caaattggca
     1261 cagcaacata ttcagcaaat ccaccatctc tgcgagaacc aacataatca taattagcac
     1321 acatttcgta tttcttttcc atacattgag aacattttcg acaaggtatt aatgggaaaa
     1381 caccaacctt ctttccaact agatttgaat atttattttc tccaacagaa aatacttcac
     1441 cagaaaattc atgaccagga attgtaggaa aatgatatgt tccttttttg aatattcgtg
     1501 gaatatcaga actacatata cctgctgcgt aaacttttac tatacaccaa tcgctgcaag
     1561 gtattttggg aatttcaata tcttcataac gaagatcatt cacggcatat aaacgatatg
     1621 ctttcatcct attttatttc cataatttgc ttgaaggtcg ttaaatcttc aggcgtagtt
     1681 atcttaaaat taatctcaga cccttctgct agtttgattt ttagtccggt tttaactgct
     1741 atttcagtgc ttccattaat tttagcaatt tcttcgtgag atatatcttt atgtatgtta
     1801 agatatttgc caagtttgaa actctctgga gcttgacctg caaataattc attcctgttc
     1861 aatagatttg ttatacttga accattatta ctttgatata ttgtatcttt tacatgaata
     1921 acaggaagaa cgccgtcata accttcatta ataaaattaa tgcattgatc aataatatca
     1981 ttagtaacaa gcggtctggc tgcatcatgt atgataacaa tgtcatcatc tgaataaccg
     2041 gctttttttg ttgcaagaag tgcattatat atagaaaatt ggcgagtttt cccagaatgt
     2101 gcataataga taggtttatc tatatgtgac atgtattttt ttacataatt tctccactca
     2161 tcagcaacca ctataatgat agcatcaata tccttgcgtt ttgcaaatgt attaacacaa
     2221 tagtaaaaaa taggtttctc ttttaccttt aaatattgtt taggag
//
LOCUS       DAOMTA010000521         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2261_length_2265_cov_1.961086, whole genome shotgun sequence.
ACCESSION   DAOMTA010000521 DAOMTA010000000
VERSION     DAOMTA010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_2265_cov_1.961086"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(664..981)
                     /locus_tag="PLK68_12365"
     CDS             complement(664..981)
                     /locus_tag="PLK68_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRM91238.1"
                     /translation="MKYNIIRTDKADEGIRHTVLYLANHFGNEFALEKLDYIENTILL
                     LEDNPYIGTLPRYAVLKRQGYRVLILEKNLAFYKVNEEKKEVVIYAFVDQRQDYLNII
                     RGL"
     gene            complement(983..1261)
                     /locus_tag="PLK68_12370"
     CDS             complement(983..1261)
                     /locus_tag="PLK68_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM91239.1"
                     /translation="MASLRETIRPSADLRNHYNEISKQCREEKEAVIITVHGKGDTVS
                     LSFEEYQNMKAHIELLETLAEAEDDVRNGRVAPINETFDDLRMILKGK"
     gene            complement(1378..1728)
                     /locus_tag="PLK68_12375"
     CDS             complement(1378..1728)
                     /locus_tag="PLK68_12375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM91240.1"
                     /translation="MIDNICRGELYIADLNPYEGCEQGGIRPVLIVQNDKGNKFSTTT
                     IVAPITSNIYTKKHIPTHFTIDYALDRKSIVLFEQIRVIDKNRILRYIGKLNSKDLKN
                     IDNKIAISLGIEKV"
     gene            1924..>2265
                     /locus_tag="PLK68_12380"
     CDS             1924..>2265
                     /locus_tag="PLK68_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91241.1"
                     /translation="MIKSHKDGIKEKSIDLHRYYPDKYKKNTYYDVPEEVADLLEKER
                     KEENCSISQYRYYVKYTLDLNDYISRTYLLTNNSPETILKKLEESDEKKINEDIQNKN
                     VEIIYCILDELN"
BASE COUNT          761 a          348 c          245 g          911 t
ORIGIN      
        1 taacgctcga attaaggcta atcatgagta tcattatttt tgatttaaca atccggtatg
       61 tattgatatt acctttattc agttttcaag gttctctcta gggttatttc aatcccttca
      121 ctatactgat aaaactcata aatttcgtgc atcattttca ctaatttctt atactttttt
      181 aatacttcaa tttatagtaa ttttcatacc taaatcttct tgtaaagaca gttccatgtt
      241 ttaaattatt ttgctataga atttttccat atataagtca agcaaaaaaa gaacaatgct
      301 tcagttatac tgtctcattg ttcttttttc tatttttgga tatttatttt attttgttaa
      361 agtacctttt cgtttgatag cattattata ttattggttg taattttttt gaataacatt
      421 atttaagtat tacttttaaa agattattag atttttcaaa tttattatct agttaaacat
      481 atcacttagt gaaatccctg aatagtgttt cactgtatca aaaagacatg atttgctttt
      541 ctaatgatct gttacgttta taattgttcc ttaactaagt attcacttta tcttctaact
      601 catcatgtac atccagatat tcatttttat caacacaact agaatataaa acccttttta
      661 gccttataaa cctctaataa tattcaaata atcttgtctt tgatcaacaa acgcataaat
      721 aacaacttct tttttttctt cattaacttt atagaatgct aaattctttt ctaatattag
      781 aactctgtaa ccttgtcttt ttaatactgc atatctaggc aacgttccaa tatagggatt
      841 atcttctaaa agtaagatag tgttttcaat ataatctagc ttttctaaag caaattcatt
      901 tccaaaatga ttcgctaaat acaaaacagt atgtctaatt ccttcgtctg ccttatctgt
      961 tcgaataata ttatatttca tactatttac cttttaatat cattcttaaa tcatcaaacg
     1021 tttcatttat aggagcaact cgtccatttc ttacatcatc ttcagcttca gctaatgttt
     1081 ctaataattc aatatgagcc ttcatatttt gatattcttc aaaagaaaga gatacagtat
     1141 ctcccttacc atgtacagta atgattaccg cttctttttc ttctctgcat tgtttagaaa
     1201 tttcattata atgatttctt aaatccgcag atggtctaat agtttctctt aagcttgcca
     1261 taaaaatccc tcctatttca ttatagacaa attgtatcat ttaatgtcta tatattcaat
     1321 gtgttcttga gtattgaata caaagaatag aattttttac atcattacca ttaattttta
     1381 tactttttca atacctaaac tgatagcaat tttattatca atatttttta aatcttttga
     1441 atttaatttt cctatgtatc ttaatattct atttttatca attactctta tctgctcaaa
     1501 tagtacaatt gattttctat ctaatgcgta gtcaatcgta aagtgtgtag gaatatgctt
     1561 ctttgtatag atattgctcg ttataggagc aactattgtt gttgtactga atttgtttcc
     1621 cttgtcattt tggactatca aaactggtct tattcctcct tgctcgcatc cctcatatgg
     1681 attcaaatct gcaatataca attctcccct gcaaatgttg tctatcataa tttttcacct
     1741 cctataattc agtgaaaaat gtttattttc gtgcacataa tcataatata ttttccctta
     1801 atatgtggtt attttcccat aggtatttct aatttctaaa tttctaataa aataaattaa
     1861 ttacattgaa aatttcatgc acattttttg tttttttcct ttgtattata ggaggaaaaa
     1921 aacatgataa aatcacacaa agatggaata aaagaaaaga gtatagattt acataggtat
     1981 taccctgaca agtataaaaa aaacacatat tatgatgttc cagaagaagt ggcagattta
     2041 ttagaaaaag aaagaaagga agaaaattgt tcaatcagtc aatacaggta ttatgtaaaa
     2101 tacaccttag atttaaatga ttatatttca cgtacatatt tattaacaaa taattcacct
     2161 gagacaatat taaaaaaatt agaagaatca gatgagaaga aaataaatga agatatacaa
     2221 aataaaaatg ttgaaattat atactgcatt ctagatgaat taaat
//
LOCUS       DAOMTA010000522         2262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2265_length_2262_cov_2.414137, whole genome shotgun sequence.
ACCESSION   DAOMTA010000522 DAOMTA010000000
VERSION     DAOMTA010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_2262_cov_2.414137"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1038
                     /locus_tag="PLK68_12385"
     CDS             277..1038
                     /locus_tag="PLK68_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRM91242.1"
                     /translation="MSNIEKLKNELIEQRKTKFPGNIYNATQVLFAYNSNKIEGSRLT
                     EDQIEMIFETGSFIAKNHDVIKADDITEAVNHFRLFDYILDMIDKPLTKEMIIDMNKI
                     LKRGTSDEFNPRYNVGGFKVLPNVIGLTNLIKTSRPENVEKDINDLLFEYENRRNIDI
                     EDIIDFHLKFERIHPFGDGNGRVGRAIMFKECLRKNIVPFVILDQHKVYYLRGLKEYD
                     RDKNFLIDTCLNEQDIYKDLCKQLLNMNFENGKTR"
     assembly_gap    1309..1318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1349..1693
                     /locus_tag="PLK68_12390"
     CDS             1349..1693
                     /locus_tag="PLK68_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91243.1"
                     /translation="MAKEITSYRFDNETLFKLEELTNYYKKNDKKTNKSKVLSSIIAD
                     AYDNINIIQKNDFSNNELEKANREIIELEYTIIKLLTTNLKEVDDIEEIKRIINAKSR
                     FQKYVEDKLSEK"
     gene            1683..2069
                     /locus_tag="PLK68_12395"
     CDS             1683..2069
                     /locus_tag="PLK68_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91244.1"
                     /translation="MKNKSLKIRIDRDLDNKINYLLNDKKNEQAKYSDDTNVTKTDII
                     TDAVELLFSYRTRDAFRLLNEEQEAYLKGVLKQYQSLNNEFFNNLIFEILILKQLIVS
                     TNGITESQLDETVKKVLDNITNANSN"
BASE COUNT          924 a          240 c          411 g          677 t
ORIGIN      
        1 ccaccggcac agggaataaa gattgagatg gaatattaaa aaagtaatca tagtagaggt
       61 gaaaaaagct aacaaagaca gtctttataa aagggaggag tacgaggagg gtgtccctcc
      121 tcggtgggtt aaaatttata aaaaggaggt gtcgagagga ggaaaacatt tcctccttcg
      181 tggcactttt taaaattaga atgtatcaaa aatcttcaaa atttactatt attttcaaaa
      241 atcaatctat ataattataa taacggagag acacgtatga gcaatatcga aaagttaaaa
      301 aatgaattaa ttgaacagag aaaaacaaaa tttcctggta atatatataa tgccacacag
      361 gtattattcg catataattc aaacaaaatt gagggcagta gattaactga ggatcagatc
      421 gaaatgattt ttgaaaccgg ttcttttatt gctaaaaatc acgatgttat taaggcggat
      481 gatattactg aagcagttaa tcacttcaga ttatttgatt acatattaga tatgatagat
      541 aaacctttaa ctaaagaaat gattatagat atgaataaaa ttttaaagcg tggcacatcc
      601 gatgagttta acccaagata taatgttgga ggatttaaag tactgccaaa tgttatcgga
      661 ttaacaaatt taattaaaac atcaagaccg gaaaatgttg aaaaggatat aaatgatttg
      721 ctttttgagt atgaaaatag aagaaatatt gatatagaag atatcataga ttttcatttg
      781 aaatttgaac gtattcatcc ttttggagat ggtaatggca gagttggacg tgcaattatg
      841 tttaaggaat gtttacgaaa gaacattgta ccttttgtta ttcttgatca gcataaggtg
      901 tattacttgc gtggattaaa agaatatgat cgagataaaa atttcctaat tgatacgtgt
      961 ctaaatgagc aggatattta taaggacttg tgcaaacagc tgttaaatat gaattttgaa
     1021 aatggtaaaa caagataaac acaatcatca gtaatatcgt tgatgattgt gtttttattt
     1081 gatatatgag aaagtatatg tattacaaat atgtaataca tggctgtaat acagatgctt
     1141 tcttaccagg ttaaggaacg aaggggtaca aatttaaaaa tttgtattac atcgttcatt
     1201 tgaacaactg agcaacgctc agttttttta tgagaaaatg tattacatac ttggggtaca
     1261 tatgtatacg aaaaaaatgg gaatcgtaat acaaaaaaag atttgttgnn nnnnnnnnct
     1321 taatcatatt ttaaggagga tgtaaaaaat ggcaaaagaa attacatcat atagatttga
     1381 taatgaaacc ttgttcaaat tagaagaact aacaaattat tataaaaaaa atgataaaaa
     1441 aactaataaa tcaaaagtgt tgagcagcat catagcagat gcttatgata atataaacat
     1501 tattcaaaaa aatgattttt cgaataatga gttggagaaa gccaatagag aaataattga
     1561 gctggagtat acaataatta agttattaac taccaattta aaagaagtag atgatataga
     1621 agaaatcaaa agaataataa atgcaaaatc aagatttcaa aaatatgtgg aggataagct
     1681 aagtgaaaaa taaaagttta aaaatccgta tagatagaga tttagataat aaaatcaatt
     1741 atttgttaaa tgataaaaaa aacgaacagg caaaatatag tgatgataca aacgtaacca
     1801 aaactgacat aataacagat gcagttgaac ttctcttttc gtatcgaact agagatgcat
     1861 tcagattatt aaatgaagaa caggaggcat atctgaaagg agtgctaaag cagtatcaaa
     1921 gtcttaataa tgaatttttt aataatctga tatttgaaat tcttattttg aaacaattga
     1981 tagttagcac taatggcata acagaaagtc aattggatga aacagtaaaa aaggtgcttg
     2041 ataatattac aaatgcaaat agcaactgat aatgtgttgt taaagataaa gtggaataac
     2101 aatagcaata aatagaggta gaatatttgt tattcttttg ttgagaggtg gtattttatg
     2161 gcaaggtcta atgcaccgat tataggtgat gtgagatttt taaaatatgg tgctgatgca
     2221 cctgatgaat caagattcaa aggaaagaca agtacagaat ct
//
LOCUS       DAOMTA010000523         2262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2268_length_2262_cov_2.074309, whole genome shotgun sequence.
ACCESSION   DAOMTA010000523 DAOMTA010000000
VERSION     DAOMTA010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_2262_cov_2.074309"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLK68_12400"
     CDS             complement(<1..639)
                     /locus_tag="PLK68_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91245.1"
                     /translation="MGINLKLSTATLITCSVMIVGCSKDQNVYNDNLQEMAKAAFPVQ
                     NIDPDQTWNMMGTTTVNTTVDENYGESYTIKIYSSDPIGNTSAQLLSKSTVDNGKSVK
                     QTFEYGTGDSTLYVSRVDSKSRREVLPVSISNGGTSNVVFSNSSISQSKSRTITRAIG
                     DFYDPTVVGKRDIPYIESQLENFCKASDAIQITDGMDVASQNIKKTNYQTFYI"
     gene            993..2045
                     /locus_tag="PLK68_12405"
     CDS             993..2045
                     /locus_tag="PLK68_12405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91246.1"
                     /translation="MGHKVFVCYYDYDNNGNKVRYIDDNIIHNYGNNFLSGIIQRIDF
                     SKISKFAIENNIEFVYSRSFINANPFTIDLFRKFKEHGIKSVIEIPTYPYDHEYDGAT
                     RKEKFELVVDKLFRKQLAKQTDRIVTFTDQKEIFGQKTCCISNGIDFDCVPVKNNKTN
                     NENRIDFIGVAEVHYWHGFDRFIHGMGEYYKNGGTYNIHFHIVGGIADREMHDSRFAR
                     GFKDLTDEYNINDKVIYHGLLFGKELDKIFNECDFAIGSLARHRSGVNKIMTLKNREY
                     AARGIPFIYSETDEDFDNKPYIIKAPADESAIDIKKVIDFYECLSITPEEIRRSIKDL
                     SWKNQMDKVIKRISNE"
     gene            2038..>2262
                     /locus_tag="PLK68_12410"
     CDS             2038..>2262
                     /locus_tag="PLK68_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91247.1"
                     /translation="MNKKYKIVYCTPSLYIPGGIERVLTTKANYFADVLDYDIYIIIT
                     DGKRKEPYYPLSKKVHIIQLNINFEKLWELS"
BASE COUNT          831 a          311 c          363 g          757 t
ORIGIN      
        1 tatataaaat gtctgataat tcgttttttt tatattttga gatgcgacat ccattccatc
       61 tgtaatctgt atggcatcac ttgctttaca gaagttttct aattggcttt ctatatatgg
      121 aatatcacgt ttaccaacaa ctgtaggatc ataaaagtca ccaatagccc ttgttatagt
      181 tctagatttc gattgagaaa tagatgaatt ggaaaataca acatttgatg ttccaccatt
      241 actaatagaa acaggaagaa cttcgcgcct tgattttgaa tctacacgtg aaacataaag
      301 agtgctgtca cctgttccat actcaaacgt ttgttttaca gatttgccat tatctacagt
      361 actctttgaa agcagttggg ctgatgtatt acctattgga tctgaagaat atatctttat
      421 agtatatgat tctccataat tttcatctac agtcgtattt acggttgttg ttcccatcat
      481 attccatgtt tgatctggat caatattctg aacaggaaaa gccgcctttg ccatctcctg
      541 taaattgtcg ttataaacat tttgatcttt agaacatcct acaatcatta cagagcaagt
      601 aataagtgtt gcggttgata attttaagtt aattcccatc ttattttcgt atttagatta
      661 ataaccacat taattatgtc gcaaaaatac acaaattatt tgtattacat ccaaaaaagt
      721 ataaaatata tgatttatta ttattatttc atttaaaagc aggcatattt acatgataaa
      781 tggatgaata gcataatttt aaagcatgat ttattacaaa attcaactta ttttggaata
      841 ttgaataaaa aaatacatat ttacaagata cgaataataa aaaaatagta tatttgcaat
      901 gtatgaggat attatttcta atatatcatg gattctctga tcatagcgga ataagcaaga
      961 agatcagata ccaaatcaaa ggactgcgtg atttggggca taaagtattt gtatgttact
     1021 atgattatga caataatggg aacaaagtta ggtatattga tgataatatc atacataatt
     1081 atggtaacaa ctttttatct ggcataatac aaaggattga ttttagcaaa atttcgaagt
     1141 ttgctattga aaacaatatc gaatttgtat attctagatc attcattaac gcgaatcctt
     1201 ttactatcga tctattcaga aaattcaaag aacatggtat taaatcagta attgaaatac
     1261 ccacatatcc gtatgatcat gaatatgatg gcgcaacaag aaaagaaaag tttgaattag
     1321 ttgttgacaa actttttcgc aagcaacttg ccaaacaaac tgatagaatt gttactttta
     1381 ctgaccaaaa agaaatattt ggacaaaaaa catgttgcat atcaaacggc attgactttg
     1441 attgtgtccc ggttaagaat aacaaaacaa acaatgaaaa tagaatagat ttcatcggtg
     1501 tagctgaagt ccactattgg catggattcg accgttttat acatggtatg ggtgaatatt
     1561 ataagaatgg aggaacatat aatatacatt ttcatattgt aggcggtata gctgatcgtg
     1621 aaatgcatga ttcacgattt gcaagaggat ttaaagatct tactgatgaa tataatataa
     1681 acgataaagt aatttatcat ggtctattat tcggaaaaga acttgataag atattcaatg
     1741 aatgtgattt tgctataggc agtcttgcac gacacagaag tggtgtaaat aaaataatga
     1801 ctcttaaaaa tagagaatat gctgcacgtg gaattccttt tatttattca gaaactgatg
     1861 aggattttga caacaaacca tatattataa aagcgcctgc agatgaaagt gctatagaca
     1921 taaaaaaagt aattgacttc tatgaatgtt taagtattac tccagaagag attcgacgtt
     1981 ctattaaaga tttatcgtgg aaaaatcaga tggataaggt tataaaaaga atttcaaatg
     2041 aataaaaaat ataaaattgt atattgcaca ccatctcttt atattcctgg tggaatagag
     2101 agagttttga caacaaaagc aaattatttt gcagatgtgc tagactatga tatatatatt
     2161 ataattactg atggaaaaag gaaagagcca tattatccat tatcaaaaaa agttcatatc
     2221 attcaactca atattaattt tgaaaaattg tgggagcttt ct
//
LOCUS       DAOMTA010000524         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2275_length_2260_cov_2.066213, whole genome shotgun sequence.
ACCESSION   DAOMTA010000524 DAOMTA010000000
VERSION     DAOMTA010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_2260_cov_2.066213"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..852
                     /locus_tag="PLK68_12415"
     CDS             97..852
                     /locus_tag="PLK68_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRM91248.1"
                     /translation="MTGKLIILVVSFFIYCLMGWIWESIILPLSRHQKPYNRGFLNGP
                     WIPIYGFGAMLVIVLFDIQQINYPIYTLFISGGVVACLLEYITSYVMEKLFHRRWWDY
                     SQKAFNLNGRVCLEGFICFGLFSVVAIDYVQPFFTSKLLLVDENILFVLSGVLSVLFI
                     VDTIVSTHVALDIEKKLELVKQVLEESENRLIASIEEKQNEARAYIEKQRLEWQKQQI
                     VLKSLIRQKKLFKYGHRRLIRAFPDLLKRKKDE"
     gene            855..2216
                     /locus_tag="PLK68_12420"
     CDS             855..2216
                     /locus_tag="PLK68_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91249.1"
                     /translation="MLKKYFGTKQFYRTVISIAFPIMAQQFITTFVNLIDNVMIGSIG
                     NIALTSVTVANRFFLIMNSILFGLCGAAGIYIAQFFGAKQKKNCQEVFNINMAFAVGA
                     GLLFTLIIFIFPQYAIELFSKTPIIVDEAIKYLHLAKYTYIPFAVSFTCMMALRAVGI
                     NKIQLKVGIVAVITNTALNYCLIFGNFGFFKMGIQGAAIATIIARLVEMAIYLIILYR
                     NKHFFSFDLKGIIRINVNLLTSIVRKAIPLTINEILFSLGQALIFMSYMRCDEYLVAS
                     ISVVDTVANIMFIVFGGLSSAVSIMIGNKLGANKLDEARDNARKLLCFALMVSLMIGM
                     ICFLLAPLIPNLYNVDIPIKEAIVRLVRIKSIMINIYAFNVCIFFILRAGGDVVSTMI
                     MDSGFLWAAGVLVSTLLSSFTTISLISLYIVVESLDLIKLIIATYFFKKERWVKNIAV
                     VGG"
BASE COUNT          684 a          269 c          453 g          854 t
ORIGIN      
        1 acgcaggggg tcagcggttc gatcccgcta ggctccacca attgtatatt aagtctgctt
       61 tggcagactt tttaatttat ttaaaggagg caaaatatga ctggaaagtt gataattctt
      121 gtggtttcat tctttattta ttgtttaatg gggtggattt gggaatcaat cattttgcct
      181 ttatcacggc atcaaaaacc atataatcga ggttttttaa atggtccctg gattcctatt
      241 tatggttttg gtgcaatgct ggtaattgtt ttatttgata ttcagcagat taattatcct
      301 atttatactt tatttattag tggcggggta gtagcttgtt tattagaata tattacttcg
      361 tatgtaatgg agaaactgtt ccatcgacgc tggtgggatt atagccaaaa ggcttttaat
      421 ttgaatggtc gtgtttgttt agagggtttt atttgttttg gtttatttag tgttgtggca
      481 atcgattatg ttcagccttt ttttactagt aagctattat tagttgatga aaatatttta
      541 tttgtcttaa gtggggtttt atcagttctc tttattgttg atacaattgt atcaacacat
      601 gtagcattgg atattgaaaa gaaattagaa ttagtaaaac aagtactgga agaaagtgaa
      661 aatagattga ttgcaagtat tgaggaaaaa caaaatgaag cacgtgcata tattgaaaaa
      721 cagcgtctag aatggcaaaa gcagcaaatc gtcttaaaat cattaattag acaaaagaag
      781 ttgtttaaat atgggcaccg gcgtttgata cgtgcgttcc ctgatttatt aaagagaaaa
      841 aaggatgagt gatgatgctg aagaaatatt ttggaactaa acaattctat agaactgtta
      901 ttagtattgc ttttccaatt atggctcaac agtttatcac aacgtttgta aatttaattg
      961 ataatgtaat gattggcagt attggcaata ttgcattaac ttcagtaacg gtggcaaatc
     1021 gtttttttct aattatgaac tcaattttat ttgggttatg tggagcggcg ggaatctata
     1081 ttgctcaatt tttcggggct aagcagaaga aaaattgcca agaggtattt aatattaata
     1141 tggcttttgc ggttggggca ggacttttat ttacattaat aatttttatt tttcctcaat
     1201 acgcaattga attgttttct aaaacaccga ttatagttga tgaggcgatt aaatatttac
     1261 atcttgctaa atatacatat atcccttttg ctgtttcgtt cacttgtatg atggctttac
     1321 gagcagttgg tattaacaag attcagctaa aagtggggat agtagcagta ataactaata
     1381 ccgcattaaa ctattgtttg atttttggaa attttggatt ctttaaaatg ggaattcaag
     1441 gggcggctat tgcaacgatt attgctcgac ttgttgaaat ggcaatatat ttgattattt
     1501 tatatcgaaa taaacatttc tttagttttg atttaaaagg gataattaga attaatgtca
     1561 acttattaac cagtattgtt cgtaaagcaa ttcctttgac gatcaatgaa attttgtttt
     1621 cattgggaca agctttaatt tttatgtcat atatgcgatg tgacgaatat ttagtggctt
     1681 ccatatctgt tgttgacaca gtagctaata taatgtttat tgtttttggt ggtctatctt
     1741 cagctgtgtc gatcatgata ggaaataaat taggtgctaa taaacttgat gaggctagag
     1801 ataatgcgcg taaattatta tgttttgcat tgatggtatc gttaatgatt gggatgatat
     1861 gttttttatt ggcaccactg attcctaatc tgtataatgt tgatatacca attaaagaag
     1921 caatcgtaag attggttcga attaaatcga taatgattaa tatctatgct tttaatgtct
     1981 gcatcttctt tattttaagg gcgggtggag atgttgttag tacgatgatt atggattcag
     2041 gttttttgtg ggctgctgga gtactggttt caactctatt atcgtcattt acgactattt
     2101 ctttaatttc gttatatatc gttgttgaaa gtttggattt gataaaatta ataattgcaa
     2161 cctacttctt taaaaaagaa cgctgggtta aaaatattgc agttgtagga ggttagaatg
     2221 aaaaaagata actatattat taaatcatta aatggaatgg
//
LOCUS       DAOMTA010000525         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2284_length_2255_cov_1.979545, whole genome shotgun sequence.
ACCESSION   DAOMTA010000525 DAOMTA010000000
VERSION     DAOMTA010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2284_length_2255_cov_1.979545"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /gene="pheT"
                     /locus_tag="PLK68_12425"
     CDS             complement(<1..1405)
                     /gene="pheT"
                     /locus_tag="PLK68_12425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM91250.1"
                     /translation="MDISLKVLNRYVKVDDQDPKELADKITSIGLEVEGMHDLTRGNN
                     MTIGYVKECMPHPDSDHLNVCQVEVRPGEIRQIVCGAPNVATGQKVIVANPGCDLGDG
                     FVIKESKIRGVESNGMICSIAELGLDQRLLKPEDKDGIHVLDTDAPVGAEPLEYMGLK
                     DTILEIGLTPNRADCMAMTSLAYEVAAILGREVNLPEVSFIGAEGSGISVKVETDLCP
                     FFGAKLVKGVTTKESPEWLRNALIASGIKPINNIVDISNFVMLETGQPIHMYDYDKLN
                     KKEFIIKTGFDCKEVMLDGEEYKIEPADLIVSTDAGIGCIAGVMGANSTKIDENTTNI
                     VIEAATFDGATLRETARRLNLLTDASQHYIKGALNTANSLMILERCANLLVELAEAKE
                     VYQSVTTDLKIKDCFVRVTTSKVNGVLGTKITTDEIKDIFSALKFEYTIDGEEFNVKV
                     PTYRNDITLAADLIEEVA"
     gene            complement(1410..>2255)
                     /gene="pheS"
                     /locus_tag="PLK68_12430"
     CDS             complement(1410..>2255)
                     /gene="pheS"
                     /locus_tag="PLK68_12430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM91251.1"
                     /translation="KQELSKAVEDKKEALENQAILDKINEERIDITLPSFKLDQGSLH
                     PLSTIVAELEDLFLGMGYQIAEGPEVESDYFNFELMNLPKGHPARDMQDTFYIDENTL
                     MRTHTSPVQAHTMLAANGEGPIKVICPGKTYRRDDDDATHSHQFMQCEGLVVDKNITM
                     ADLKGTLEVFARKFFGEKREVRLRPSYFPFTEPSVEVDISCHNCGGKGCPMCKHTGWI
                     EILGAGMVNPRVLEMCGFDSEVYQGFAFGIGIERVAMLKYGIDNIRNFYNDDIRFLSQ
                     FGRKE"
BASE COUNT          701 a          478 c          279 g          797 t
ORIGIN      
        1 gtgctacttc ttcaattaaa tctgcagcta aagtaatatc attacgataa gttggtactt
       61 tcacattaaa ttcttcacca tcaatagtat actcaaattt tagtgcactg aaaatatctt
      121 tgatttcatc agttgtaatt ttagttccaa gtaccccatt tacttttgat gttgttactc
      181 gaacaaaaca gtctttgatt tttagatcag ttgttacact ttgatatact tcttttgctt
      241 ctgctaattc aactaataaa ttagcacatc tttctaaaat cattaaagaa tttgcagtat
      301 ttaaagcacc tttaatataa tgttgtgagg catcagtcaa taagtttaaa cgtcgagcag
      361 tttctcttaa tgttgcccca tcaaacgttg ctgcttcaat aacaatattg gttgtattct
      421 catcaatttt agtactatta gcacccataa ctccagcaat acaaccaatt ccagcatcag
      481 tggatacaat aaggtcagct ggttcaattt tatattcctc accatctaac attacttctt
      541 tacaatcaaa tcctgtttta ataataaatt cctttttatt taatttatca taatcataca
      601 tatgaattgg ttgtcctgtt tctaacatta caaaattaga aatatcaaca atattattaa
      661 ttggtttgat tccactagca ataagtgcat ttcttagcca ttcaggagat tctttagttg
      721 ttactccctt aactaattta gcaccaaaaa atggacataa atcagtttca actttaacgc
      781 tgattccact tccctctgct ccgataaatg aaacttctgg taaattaact tcacgaccta
      841 aaattgctgc tacttcataa gctaaagaag tcattgccat acaatcagca cgatttggag
      901 tcaaaccaat ttccaaaatc gtatctttta atcccatata ttctaatggc tcggctccaa
      961 ctggagcatc agtgtctaaa acatgaattc catctttatc ttctggtttt aaaagtcttt
     1021 gatctaaacc gagttcagca attgaacaaa tcattccatt cgactcaact cccctgatct
     1081 tactctcttt aataacaaaa ccatcaccta aatcacatcc tggattagct acaattactt
     1141 tttgtccagt tgccacattt ggtgccccac aaacaatctg tctaatttca ccaggtctaa
     1201 cttcaacttg gcatacattt aaatgatcac tgtctggatg tggcatacac tctttaacat
     1261 aaccaattgt catgttattt cctctagtca aatcgtgcat tccttcaact tccaatccaa
     1321 ttgaagtaat tttatctgct aattctttag gatcttgatc atcaaccttt acatatctat
     1381 ttaatacctt taaactaata tccatctttt tactcctttc taccaaactg acttaaaaat
     1441 ctaatatcat cattatagaa attgcggata ttatcaatgc cgtatttaag cattgcaacc
     1501 cgttcaattc caattccaaa tgcaaatccc tggtatactt cagagtcaaa tccgcacatt
     1561 tctaaaacac gtgggttaac cattccagct cctaagattt ctatccatcc tgtatgttta
     1621 cacattggac aacctttacc accacagtta tgacatgaaa tatcaacttc tactgaaggc
     1681 tcagtaaatg gaaaatacga tggacgtaag cgaacctcac gcttttcacc aaagaacttg
     1741 cgtgcaaata cttctaaagt tcctttcaaa tcagccatgg taatattttt atcaactact
     1801 aatccttcac actgcatgaa ttgatgtgaa tgcgttgcat catcatcatc acggcgatat
     1861 gttttacctg gacaaattac tttgattggt ccttcaccat ttgcagctaa cattgtatgt
     1921 gcttgaactg gtgacgtatg agtccgcatt aaagtatttt catcaatata gaatgtatct
     1981 tgcatatcac gagctggatg accttttggt aaattcatta attcaaagtt gaaataatca
     2041 ctttctacct caggaccttc agcaatttga tatcccattc ccaaaaacaa atcttctaat
     2101 tctgctacaa ttgttgataa aggatgcaaa gacccttggt ctaatttaaa gcttggtaaa
     2161 gtaatatcta ttctttcttc gttgatttta tctaaaatag cttgattttc caaagcttct
     2221 tttttatctt caactgcttt tgataattct tgctt
//
LOCUS       DAOMTA010000526         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2285_length_2255_cov_1.870909, whole genome shotgun sequence.
ACCESSION   DAOMTA010000526 DAOMTA010000000
VERSION     DAOMTA010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_2255_cov_1.870909"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLK68_12435"
     CDS             <1..266
                     /locus_tag="PLK68_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM91252.1"
                     /translation="QRLKQIEPLILEMKNIGLKYNVGVAEIAIAYALAKNTVPIIGVT
                     KVKHIDSALAALKITLSEEDIHKLEYFSNNFQLKIKGFWEKKM"
     gene            266..700
                     /locus_tag="PLK68_12440"
     CDS             266..700
                     /locus_tag="PLK68_12440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91253.1"
                     /translation="MIVVRVMTIKDYDDVYELWMNTKGMGMRNLDDSRSGIAKFLERN
                     PTTNFIASDGNKIVGVILAGNDGRRAYIYHTTVRSDYRGQGIATQLVKECLSAVKAEG
                     INKTALVVFADNQLGNDFWQSQGFKEREDLTYRDFSLNEENI"
     gene            700..981
                     /locus_tag="PLK68_12445"
     CDS             700..981
                     /locus_tag="PLK68_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91254.1"
                     /translation="MRIQQVDITEVFFQTKEYTQGMYDSIVRIGLSFPVKVSLEQGKF
                     YCQDGHKRLSAIANMEKLEVYKKRRKINIIVINNGNSRSNDCWRGRNMH"
     gene            1230..>2255
                     /locus_tag="PLK68_12450"
     CDS             1230..>2255
                     /locus_tag="PLK68_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM91255.1"
                     /translation="MYYNREIKELAQEFSTDLKSGLTEQQVTENLAKYGPNELEQTKK
                     QSIIVRFFLQFKDALTIILIIAAVVSIIVEPTEWIDSAIIVFVVVVNAILGLVQENNA
                     ERALEALQKMASPKAKVIREGNIITINASQVVPGDLLVLEAGDCIASDGRLVECFNLK
                     VDESALTGESVPVEKISSILDKEEIPLAERNNMVYASCNVTYGRGIAIVTTTGEENEV
                     GKIATMIQQTKRENTPLQDQLDQIGRTIGVICIIICIVIFGMELLEGLSILEAFKTSI
                     ALAVAAIPEGLATVVTVVLALSVQRMVKKNAIVKSLPAVETLGSTSIVCSDKTGTLTQ
                     NKMTVVKT"
BASE COUNT          773 a          271 c          502 g          709 t
ORIGIN      
        1 agcaacgttt aaaacagatt gaaccattga ttctagaaat gaaaaatatt ggattaaaat
       61 ataatgtcgg tgttgctgaa attgctattg cttatgctct ggcaaaaaat actgttccaa
      121 ttattggagt aacgaaagtt aagcatattg atagtgcttt ggctgcttta aaaataactt
      181 tgagtgaaga agacattcat aaactagaat attttagtaa taattttcaa ttaaaaatca
      241 aaggattttg ggagaagaaa atgtaatgat agtggtacga gtaatgacaa ttaaagatta
      301 cgacgatgtg tatgagcttt ggatgaatac taaaggaatg ggaatgagaa atcttgatga
      361 tagtcgtagt ggaattgcta aatttcttga acgcaatccg acaacaaact ttattgcgag
      421 tgatggtaat aaaattgttg gggtgatcct tgctggtaat gatgggcgcc gtgcttatat
      481 ttatcataca actgtgcgaa gtgattatcg tggtcaggga attgcgacgc agctagtaaa
      541 agaatgtttg tcagcggtta aagctgaggg aattaataag actgctttag ttgtttttgc
      601 ggacaatcag ctaggtaatg atttttggca gagtcaagga tttaaggagc gtgaggactt
      661 aacttatcgt gattttagtt taaatgagga gaatatttga tgaggatcca gcaggtagat
      721 ataacagagg ttttttttca aacaaaggag tatactcaag gaatgtatga ttcgattgtt
      781 cgaattggac tgtcatttcc ggtaaaagta agtttagaac aagggaaatt ttattgtcaa
      841 gatggtcata aacggttaag tgcaattgca aatatggaaa aattagaagt atataaaaaa
      901 cgtcgaaaaa taaatattat tgttattaat aatggtaata gtcgttctaa tgattgctgg
      961 agaggcagaa atatgcatta attaggatta aatatggaaa attgggaata atattgatga
     1021 tcaagaatta ttgtattagt tgctttttaa aacgcttaat gataaaataa gtatatataa
     1081 aagggagtag ttagccatta ggtttacgat gccgtcaact cgatattatt ttatatccgt
     1141 atcgtaatta aacaacgaga cttttattgt atgcaaatac gatatagtct cgttttttgc
     1201 ataatgtttt agaaaaagga gagatattta tgtattacaa tcgtgagatt aaagagttag
     1261 cccaggaatt ttcaactgat ttaaaaagtg gcttaactga acagcaagtc acagagaatt
     1321 tggcaaaata tggtccaaat gaattagaac agactaaaaa acaatctatt atagttcgat
     1381 tctttttgca gttcaaagat gcgttaacga ttattttaat tattgctgca gttgtcagca
     1441 ttattgttga accaactgaa tggatcgata gtgcaatcat tgtttttgtg gtagtagtta
     1501 atgcgatttt aggcttagtt caggaaaata atgcagaaag agcactagag gctttgcaaa
     1561 aaatggcgag tcctaaagct aaggttattc gtgagggtaa tattattacg atcaatgcca
     1621 gtcaagtcgt accaggggat ctgttagtgc ttgaagcggg tgactgtatt gcaagtgatg
     1681 ggcgattagt ggaatgtttt aaccttaaag ttgatgagag tgcactaact ggtgaaagtg
     1741 ttcctgtcga aaaaatcagc tcgattcttg ataaagaaga gatcccgtta gcagaacgaa
     1801 ataatatggt ttacgcttct tgtaatgtta catatggacg gggaatcgca attgttacca
     1861 cgactggtga agaaaatgaa gttggaaaga ttgcaacgat gattcagcag actaagcgtg
     1921 aaaatacacc gcttcaagat caattggatc aaattggaag aacaatagga gttatttgta
     1981 ttattatttg tatcgttatt tttggaatgg aactgttgga aggtctctca attcttgagg
     2041 cttttaaaac atcaattgca ttggcagttg cagcaattcc tgaagggttg gcaactgttg
     2101 ttactgtagt tcttgccttg agtgttcagc gaatggttaa aaagaatgca attgttaaaa
     2161 gtttaccagc agttgaaaca ttaggtagta cctcaatagt atgctcagat aagacgggga
     2221 ctttgactca aaataaaatg acagttgtaa aaaca
//
LOCUS       DAOMTA010000527         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2289_length_2250_cov_2.825968, whole genome shotgun sequence.
ACCESSION   DAOMTA010000527 DAOMTA010000000
VERSION     DAOMTA010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2289_length_2250_cov_2.825968"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..853)
                     /locus_tag="PLK68_12455"
     CDS             complement(41..853)
                     /locus_tag="PLK68_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91256.1"
                     /translation="MIIEHLLDDNNLTNTEKSIAQFLLNKDQKINNLTSSELGKQSYT
                     SQAAVTRLYKKLGFNNFREFLSTLILERNDYLKYNDLPTEHSEQYFTSLEDTEKVIAS
                     LYEKAMIQTNRLLDKNVVNRVCNRILSASFIDIYGIGLSDTIAKEMCFKLQSLGLPCS
                     YQNGINIKYIDNMQQHQSNVSILITTTGDNNLIKGIAKILKNRNVYTVGILGKKGKDL
                     IQLCHDYLLFDTSLFEDIDSLCATFSAEYVINILYATLLYRLELSNYMHYLK"
     gene            complement(951..1592)
                     /locus_tag="PLK68_12460"
     CDS             complement(951..1592)
                     /locus_tag="PLK68_12460"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM91257.1"
                     /translation="MKLEKKMMNKQNRWLDLAQELQFLAQGGLAYTKDKFDQERFERI
                     RQISAEMVALQSDLPIATVTSLFCNETGFQTPKLDSRGAVFKGDKILLVQESDGRWSI
                     PGGWVDALASVKENTIRELQEEAGIEAQIVKVIAILDRNIHNTPRYAYGITKIFIECS
                     YLGGAFKPNIETLDSGYFSLDELPELAEEKVTLEQIKMCFKAHFDDNWQCLCD"
     gene            complement(1555..>2250)
                     /locus_tag="PLK68_12465"
     CDS             complement(1555..>2250)
                     /locus_tag="PLK68_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRM91258.1"
                     /translation="IVLVTIVLAIIMSEITAMQSIIKALLASGGILAVVVGLASQEAA
                     SNMINGFMIMTYKPYKIGDFVNVREYNVIGTVIDISMRHSVIETLERTQVIIPNTIMN
                     KAIIENVSNVKTQKANYLYLEVSYESDIQKAIEIIQEEGSKHSLCLDGRTKAQKKKHE
                     PMVKVHCVEFNDSGIQLRATLLSKDSGAGFQLLSDLRLIIKKRFDENGIDIPYPHRVV
                     INETREKNDEQTK"
BASE COUNT          750 a          415 c          289 g          796 t
ORIGIN      
        1 atcaagcttt aaacgcatta gaaaaataat caactgacag ctattttaaa tagtgcatat
       61 aattagataa ttcaagacga tagagcaatg ttgcatataa aatattgatt acgtattcag
      121 cactaaaagt agcacataag ctatcaatat cttcaaataa actagtatca aataatagat
      181 aatcgtggca cagctgaatt agatccttac cctttttacc taatatcccg accgtatata
      241 cattcctatt ttttaagatt ttagcaattc ctttaattaa gttattatcg ccggtcgtag
      301 tgataaggat cgagacgtta ctttgatgct gctgcatatt atcaatatat ttaatattaa
      361 taccattttg atatgaacaa gggagcccta atgattgtaa tttaaaacac atttctttag
      421 caatcgtatc tgaaagacca attccataaa tatcgataaa cgaagcactc aatatccgat
      481 tacaaacacg attaacaaca tttttatcta gtaatcgatt tgtctgaatc atcgcttttt
      541 catataaaga ggcaattact ttttcagtat cttctaaaga tgtaaaatac tgttcagaat
      601 gctcagttgg gagatcatta tatttaagat aatcatttct ttctaagatc aatgtagata
      661 aaaattcacg aaaattattg aaccctaatt tcttataaag acgggttact gccgcttgtg
      721 aagtataact ctgttttccc aactcactgc ttgtaagatt attaattttt tgatctttgt
      781 ttaaaagaaa ttgagcaata cttttttcag tattggtaag gttattatca tctaataagt
      841 gttctataat catatttcgt agacctcctt taactatttg atcgtatcta tattttatca
      901 aataatcaat aaaataaaag ggttgaataa atcaacccag taattgtcta ttaatcgcat
      961 aaacattgcc aattatcatc aaaatgtgct ttgaagcaca tttttatctg ttctaaagtt
     1021 actttctcct cagccaattc aggaagttca tctaggctaa aataaccact atcaagtgtt
     1081 tcgatatttg gtttaaatgc accaccaaga taagaacatt ctatgaaaat cttagtaatt
     1141 ccatacgcat agcgaggcgt attatgaata ttacgatcta gaattgcaat tactttaact
     1201 atctgagcct caatcccggc ttcttcttgt aattccctaa ttgtattttc cttaacactt
     1261 gctaaagcat ctacccaacc accgggaatt gaccagcgtc catcagattc ctgcaccagc
     1321 aaaatcttat cacctttaaa aacagcacca cgagaatcta attttggtgt ttgaaaccct
     1381 gtttcattac aaaataatga agtaactgtt gcaatcggta aatcactttg taaagcgacc
     1441 atttctgcag atatttgacg aattcgctca aatctttctt gatcaaattt atcttttgta
     1501 tatgctaatc caccttgagc taaaaattgt aactcttgag ctaaatctaa ccacctattt
     1561 tgtttgttca tcattttttt ctctagtttc attaattaca actcgatgcg gatatggaat
     1621 atcaatcccg ttttcatcaa aacgtttctt aataatcaaa cgcaaatctg ataataattg
     1681 aaaaccagca ccactatcct tagataataa tgttgctcgt aattgaattc cactatcatt
     1741 aaattcgaca caatgaactt taaccattgg ttcatgtttt ttcttttgtg ctttagttct
     1801 gccatctaaa cataatgaat gtttacttcc ttcttcttga ataatttcaa ttgctttttg
     1861 aatatcactt tcgtaagaaa cctcaagata taaatagttg gctttttgag tcttaacatt
     1921 agatacattt tcaataattg ctttattcat tatcgtatta ggtataatta cctgtgttct
     1981 ttctaatgtt tcaatgacac tgtggcgcat tgaaatatca attacagttc caataacatt
     2041 atattctcta acattgacaa aatcaccaat tttataaggc ttataagtca tgatcataaa
     2101 accattgatc atattacttg cagcttcttg tgatgctaac ccaactacaa ctgctaagat
     2161 tcccccactg gccagcagag ccttaatgat tgactgcatc gccgtaattt cactcataat
     2221 aattgctaaa acaattgtta ctaaaacgat
//
LOCUS       DAOMTA010000528         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2295_length_2247_cov_2.264599, whole genome shotgun sequence.
ACCESSION   DAOMTA010000528 DAOMTA010000000
VERSION     DAOMTA010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_2247_cov_2.264599"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLK68_12470"
     CDS             <1..374
                     /locus_tag="PLK68_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formamidopyrimidine-DNA glycosylase"
                     /protein_id="HRM91259.1"
                     /translation="LITDETEKLSIKAFLATKQRILGIGNGVAQDIMFNAKLFPKRRI
                     KTLNEQDIKNLYDAIIRTLTKMVENHGRDSEKDIYGNPGGYKTILCAKSYKSGCPICH
                     CEIKKEQYLGGSIYYCPNCQK"
     gene            complement(608..>902)
                     /locus_tag="PLK68_12475"
     CDS             complement(608..>902)
                     /locus_tag="PLK68_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyltransferase"
                     /protein_id="HRM91260.1"
                     /translation="FFSEAMREYNNHLIDNWQAQKRYQTIKEAILTDKINDFNDAAAL
                     LSGRYGYICQYDRKTGKDTVWSVIYDVKNKRIFRVEGNPSRKSFMEDKRFKFK"
     assembly_gap    903..912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<913..1614)
                     /locus_tag="PLK68_12480"
     CDS             complement(<913..1614)
                     /locus_tag="PLK68_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRM91261.1"
                     /translation="MYHSRFKGEHYQIGFKWGRNLLKHKQLFLTNVPFEITLEHYDFA
                     LQCLPIYQKFFPEIIEEIRGIADGQKCSFKSICAVLLSMYCIVPEVHSSCFAFRNENE
                     ILFGRNSDFLTKIEKLYTNCIYQFTNDSYAFNGNTTACVEMEDGINEYGLAIGLTAVY
                     PTVKQLGLNAGMMLRLFLEKCKTVDEVIQLLNTLPIASSQTFTLVDTGGDIALIECNS
                     KQIKINRNNQNAFATN"
     gene            complement(1981..2202)
                     /locus_tag="PLK68_12485"
     CDS             complement(1981..2202)
                     /locus_tag="PLK68_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91262.1"
                     /translation="MDYYHGIYIDLDIYAEYSCHLFESISLDNIHEIFAILKDKDIII
                     EDPLNLNGIFTQYQNLSELEKYFDNNMSK"
BASE COUNT          801 a          393 c          282 g          761 t
ORIGIN      
        1 cattaattac tgatgaaact gaaaaactca gtattaaagc ctttttagca actaagcaaa
       61 gaattttagg gattggtaat ggtgttgctc aagatattat gtttaatgcc aaactctttc
      121 ccaaacgaag gattaagaca ttaaatgaac aagatataaa aaatttatat gatgcgatta
      181 ttagaactct aacaaagatg gtcgaaaacc acggtcgaga cagtgaaaaa gatatatacg
      241 gaaatcctgg tggttacaaa actatcttat gcgctaaatc ttataaaagt ggttgcccaa
      301 tctgtcactg tgaaataaaa aaagaacaat atctaggtgg tagtatctat tattgtccta
      361 attgtcaaaa ataaaaaagt aagatagaaa ttaatccaag ggctgtcagg tagatacaca
      421 aaacaaataa aatttttaac ccggctaact tagtgatatg ctcccacctc catagatacc
      481 catttggtta tttcgtgtgt ctactttagt ggaatcatca tatcatgttt tcatttactt
      541 ttttatcttt ctgtttaaat caaaataaaa acctgtttat tttcaatcag ttatagcatt
      601 catctaatca cttgaactta aatcttttat cttccataaa ggattttcgt gaaggatttc
      661 cttcaactcg aaaaatcctt ttatttttca catcatatat gaccgaccaa actgtatcct
      721 tccctgtttt tcgatcgtac tgacaaatat aaccatacct acctgataat aaagcagccg
      781 catcattaaa atcgttgatt ttatcagtta aaatcgcttc tttgatagtt tgatagcgct
      841 tttgagcttg ccaattatca ataagatgat tattatattc ccgcattgct tcactaaaga
      901 acnnnnnnnn nngttagttg caaaagcatt ctgattattt ctattgattt taatttgttt
      961 actattgcac tcaatcaatg caatatcacc accagtatca accagtgtaa aagtttgact
     1021 tgaagcaatc ggtaatgtat tcaacaattg aattacttca tcaactgttt tacacttctc
     1081 caaaaatagc cgcagcatca ttccggcatt tagccccagt tgttttacag tcggataaac
     1141 agcagttaat ccaattgcta aaccatactc gttgatacca tcttccatct caacgcacgc
     1201 tgtagtgttg ccattaaacg catatgaatc attagtaaat tgatatatac aattagtata
     1261 cagtttttca atttttgtta aaaaatcact atttcgtcca aataagattt cattttcatt
     1321 tctaaaagca aaacaagaac tatgtacttc tggaacaatg cagtacatac tcaacaaaac
     1381 agcacaaata cttttaaatg aacatttttg tccatcagca attcccctta tttcttcaat
     1441 aatctctggg aaaaattttt gataaatcgg taaacattgt aaagcaaaat catagtgctc
     1501 taaagtaatt tcaaagggta cattagttaa aaacagttgc ttatgtttta ataaatttct
     1561 cccccactta aatccaatct gataatgttc tcctttaaat cttgaatgat acataatttt
     1621 tcgctccttt ttataagagc caccggtaac tcataatcca agcataaatc aataaaattt
     1681 tattgtcaat gaattaaatt tattaaatca attacccgtt ttacttatta aaaatcatga
     1741 aattatctat ttaaaggcaa atataatcac tcctatgata aaaagagata gcggagcaat
     1801 taaaaactaa aatcaaaata gctaacgtac gtttaggaaa actactattt attaaagact
     1861 ttaatataaa taatttattt cctcttctcc ctttttcagt attatttata taagtatgaa
     1921 gcttatcaat ctgttgagaa atttcgctac ctcaagggtt atccaagcca tatttttttg
     1981 ctatttagac atattattat caaaatattt ttcaagctca gacaaatttt gatattgggt
     2041 aaaaataccg ttcaaattga gcggatcttc gattatgata tccttatctt ttaaaatagc
     2101 aaagatttca tgaatattat ctagagaaat actctcaaat aaatgacaac tatattcagc
     2161 atatatatcc aaatctatat aaataccgtg ataataatcc aatatgtgac cacattgcaa
     2221 atagcaaata cccatgatcg ccagcta
//
LOCUS       DAOMTA010000529         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2297_length_2247_cov_2.052007, whole genome shotgun sequence.
ACCESSION   DAOMTA010000529 DAOMTA010000000
VERSION     DAOMTA010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_2247_cov_2.052007"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..>711)
                     /locus_tag="PLK68_12490"
     CDS             complement(94..>711)
                     /locus_tag="PLK68_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRM91263.1"
                     /translation="KITYDGHENIIASFADIREVLNNDNLKSKNATIMLQVELVRDGK
                     TITKEVNAKYNSDSNSYTMGLTPATRNLSFFEAINYGVTKFVEMALLIFTTLGKLFTD
                     SANTIGQLSGPAGIYNVTAQITETGSISQLLTLLALLSTNIGMFNLLPIPGLDGCQVI
                     FAVVERVIGRELPLKVKYGLQIAGLALVFGLMIFVTFNDISRIFG"
     assembly_gap    712..721
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<722..1180)
                     /locus_tag="PLK68_12495"
     CDS             complement(<722..1180)
                     /locus_tag="PLK68_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRM91264.1"
                     /translation="MQTLINIVVFILILGIVVLIHELGHFITAKSFGVYCSEFSIGMG
                     PKIFSRKKGETEYEIRALPIGGFVSMAGEADNDIEEFKDVPIERTLKGISCWKKCVVF
                     LAGVFMNFVLSLVILIGVYCVIDVQTNTPEIGKVTSDSPAMIAGLEAGDTI"
     gene            complement(1180..>2247)
                     /locus_tag="PLK68_12500"
     CDS             complement(1180..>2247)
                     /locus_tag="PLK68_12500"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRM91265.1"
                     /translation="KITAMAAGYNVAKVEEILAMIDVEYICMVKKEDALYLQEKYPDL
                     KVVYGESGLIEIATLPEIEIVLNAIVGFAGLVPTIEAIKAKKDIALANKETLVVAGHI
                     ITELVKEYGVKLLPVDSEHSAIFQSLNGEEHNKIKKIILTASGGSFRDKQRDELAGVT
                     VKEALNHPNWSMGAKITIDSATLFNKGLEVMEAKWLFDVDYDQIEVLIHPESIIHSMV
                     EFVDTSIIGQLGNPDMRLPIQYALTYPERDYLIGGESLDLAKIASLTFKKPDFERFRA
                     LALAYQAGKSGGSMPCVLNGANEQANELFRNGKIEFLEIENLVEKALNKHQLVKNPTL
                     EQLIEIDAWARNFVLKEIGED"
BASE COUNT          749 a          467 c          274 g          747 t
ORIGIN      
        1 taataattta ttttccatca aatcatcctt cattattctt atcaatataa cactcaaaca
       61 ttatagcata aaaaaaggga aaattaattc cctttatcca aatattctag aaatatcatt
      121 aaaggttaca aagatcatta gaccaaaaac aagggctaat ccagcaattt gtaaaccata
      181 tttaaccttt aacggtaatt cacgcccgat tactctttca accacagcaa agataacttg
      241 acaaccgtct aaaccaggaa taggcaataa attgaacatt ccaatattag tcgataataa
      301 agctagcaat gttaataatt gactaattga tcctgtttca gtaatttgag ccgttacgtt
      361 gtaaattccc gctggtcctg acaattgtcc aattgtatta gcggagtctg taaatagctt
      421 acctaatgtt gtaaaaatca acagcgccat ctctacaaat ttagttactc cataattaat
      481 agcttcaaaa aagcttaaat ttcttgttgc tggagtcaaa cccattgtgt atgaattaga
      541 atcactatta tattttgcat taacttcttt agtaattgtt ttaccatcac ggaccaattc
      601 aacttgtaac attattgtag cattttttga tttaagatta tcattattca acacttcacg
      661 aatgtctgca aaagatgcaa taatattttc atgaccatca taagtaattt tnnnnnnnnn
      721 naatagtatc tcccgcctca aggcctgcaa tcattgcggg actgtccgaa gtaactttcc
      781 cgatttcagg tgtattagtt tgaacatcta ttacacaata aacaccaatt aaaattacta
      841 atgatagaac aaaattcata aagactccgg ctaaaaaaac aacacacttt ttccagcagc
      901 taatgccctt taaagtacgc tcaattggaa catctttaaa ttcctcaata tcattatcag
      961 cctctccagc cattgataca aaaccaccaa ttggtaaagc tctgatttca tattctgtct
     1021 cacctttttt gcgcgaaaat atctttggtc ccatcccaat tgaaaattca ctacaataaa
     1081 ccccaaacga tttagccgta ataaaatgtc ctaactcatg gatcagcaca acgattccaa
     1141 gaatcaaaat aaaaacgaca atattaatta atgtctgcat taatcttctc ctatctcttt
     1201 taaaacaaag tttcgagccc aagcatcgat ttcaatcaac tgttcaagtg ttggattttt
     1261 gactaactga tgtttattca atgctttctc aactaaattt tcaatttcaa gaaactcaat
     1321 tttaccattt ctaaatagct catttgcttg ttcatttgca ccatttaaca cacacggcat
     1381 acttccacca cttttgccgg cttgataagc taaagctaat gctctaaaac gttcaaaatc
     1441 tggcttttta aatgttaatg atgcaatctt tgctaaatca agactttcac caccaattaa
     1501 ataatcacgt tctggataag ttaaagcata ttgaattggt aaccgcatat ctggattgcc
     1561 taattgtccg ataattgaag tatccacaaa ttcaaccatc gaatgaatga tgctttcagg
     1621 atgaattaaa acttcaattt gatcataatc cacgtcaaat aaccattttg cttccattac
     1681 ttccaaccct ttgttaaata aagttgcaga atcaattgta attttagctc ccattgacca
     1741 attcgggtga tttaatgctt ctttaacagt tacacctgct aattcatcac gttgcttatc
     1801 acgaaagctg ccaccactag cagttaaaat aatcttctta attttattgt gttcttcgcc
     1861 atttaatgat tgaaaaatag cagagtgttc actatcaacc ggtagaagtt tgacgccata
     1921 ttcttttacc agttcagtaa ttatatgccc cgctacgact aaagtttcct tattcgccaa
     1981 agctatatct ttttttgctt taattgcttc aattgttggg actaaaccag caaatccgac
     2041 aattgcattt aatacaatct caatctctgg taaagttgca atttctatca atccactttc
     2101 accataaaca actttaagat caggatattt ttcttgaaga tataaagcat cttctttttt
     2161 taccatacag atatactcaa catcaatcat tgctaaaatt tcctcaacct tagccacatt
     2221 ataccctgct gccattgctg taatctt
//
LOCUS       DAOMTA010000530         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2298_length_2247_cov_1.929745, whole genome shotgun sequence.
ACCESSION   DAOMTA010000530 DAOMTA010000000
VERSION     DAOMTA010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_2247_cov_1.929745"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLK68_12505"
     CDS             complement(<1..607)
                     /locus_tag="PLK68_12505"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRM91266.1"
                     /translation="MKRNETRTIKVGNLTIGGNNEVIIQSMTNTKTKNIEATVKQINA
                     LAATGCQLVRLAVLNNDDALAIKEIKKHVSIPLVADIHFDYRLALQAIESGIDKIRIN
                     PGNIGSPEKVEMVVNACKKHHVPIRIGVNSGSLEKDILAKYGKPTAEGMIESAKKHVE
                     ILESLGFYDICISLKSSGIKKTSKSISKNISIYCHVVLSPAE"
     gene            817..1548
                     /locus_tag="PLK68_12510"
     CDS             817..1548
                     /locus_tag="PLK68_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRM91267.1"
                     /translation="MENSKEEKVTSVSTENIYKLNGRVPLKKAIPFGLQHVLAMFVSN
                     LAPVLIVCGAALVRGTGEHLTSAEITQLLQCAMFVAGIGTCMQLYPVWKIGSKLPIVM
                     GVSFTFLGSLLVICTNPDLGYEGMIGAVILGGIFEGIVGLSAKYWKRFLTPVVSACVV
                     IAIGLSLLSVGMDSWGGGNGAADFGSWYHLLVGTFTLVVCLVSRYLLKGVYKNLNILV
                     GLIFGYLLSVIFTVANIARWWIFQE"
     gene            1527..2222
                     /locus_tag="PLK68_12515"
     CDS             1527..2222
                     /locus_tag="PLK68_12515"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRM91268.1"
                     /translation="MVDFSGITKTIQEVGFFSIPKLVFFTSHTPVFDLGAFFTIAIVF
                     LVSAAETTGATTAVCTGALERDIKMEELQGSLAVDGLSSAISGCFGCIPLTSFSQNVG
                     LVTMTKVINRFTILVGALILILASLFPPLGAFFNSLPQAVLGGCTVMMFGSIMYEGIK
                     MLKENEFDERTMIIVSLAFCIGVGLTQTSGNFFAAFPPVVGDIFNGNAVAGVFVVSLL
                     LSLVLPKKKEAKK"
BASE COUNT          655 a          318 c          447 g          827 t
ORIGIN      
        1 gttctgccgg agaaagaaca acatggcagt atattgatat gtttttggaa atggatttcg
       61 atgttttttt tatgcctgat gattttaaag aaatacagat atcatagaaa cctaggcttt
      121 ctaatatttc aacatgcttt ttcgcacttt caatcattcc ctcagcagta ggtttgccat
      181 atttagctaa aatatctttt tctaaagaac cactattgac tccgattcga attggaacgt
      241 gatgtttctt acaggcattt acaaccattt caaccttttc tggagaaccg atattaccag
      301 ggttgattct aattttatca attcctgatt caattgcctg taatgctaaa cgataatcaa
      361 aatgaatatc agcgactagc ggaatactaa catgtttttt tatttcttta atagctagag
      421 cgtcatcatt atttaaaact gcaagtcgca ctaattgaca gccagttgca gctagagcat
      481 ttatttgttt cacagttgct tcaatgtttt tagttttagt atttgtcatt gactggataa
      541 ttacttcgtt attaccacca atagtaaggt ttcccacttt gatagttctt gtttcatttc
      601 tcttcatgaa tttcacctcg ttagaattat aacatatcct tccttattaa taaaaataaa
      661 tgttctatta tatggcttta ttaaagaaaa ggcttactag ggagtagata tttgtctatt
      721 tttatggtaa aattctatcg ttttttatag ttattatcgt ttggatagat agcagttata
      781 acttcaagaa gataaaagaa aataggggtc attatgatgg aaaatagtaa agaagaaaag
      841 gttacatctg taagtacaga aaatatttac aagctaaatg gaagagttcc tttaaaaaaa
      901 gcaattcctt ttgggttgca gcatgtttta gcgatgtttg tttcgaattt agcacctgta
      961 ttgattgtat gtggtgcagc acttgtaaga ggaacaggag aacatttaac ttcagctgag
     1021 atcacgcaat tgctacaatg tgcaatgttt gtagctggga ttggaacctg catgcaactt
     1081 tatccagttt ggaagattgg ttcgaaatta cctattgtta tgggtgttag tttcactttt
     1141 ttaggaagtt tattagtaat ttgtacgaac ccagacttag gatatgaagg gatgatcgga
     1201 gcggtcatct taggtgggat ctttgaggga attgtcggat tgagtgcaaa atactggaaa
     1261 cgttttttaa ctccagttgt atcagcttgt gtagtaattg caattggact ttcactttta
     1321 tcagttggaa tggattcatg gggcggtgga aatggtgctg ctgattttgg gtcatggtat
     1381 catttattag ttggtacatt cactttagtt gtctgtttag tatcacgtta tttactgaaa
     1441 ggtgtttata aaaatttaaa tattttagtg ggacttattt ttggatattt actatctgtt
     1501 atttttacgg tcgcaaatat tgctcgatgg tggatttttc aggaataaca aaaacaatac
     1561 aagaagtagg tttttttagt attcccaagt tagttttttt tacgagtcat acaccagttt
     1621 ttgatttagg agcattcttc actattgcaa tcgtattttt ggtatctgct gcagaaacaa
     1681 cgggagcgac tacagcagtg tgtaccggtg ctttagaacg tgatattaag atggaggaac
     1741 tacaaggttc acttgctgtt gatggccttt ctagcgcaat ttcagggtgt tttggttgta
     1801 ttcctttaac atctttcagt caaaatgtgg gattagttac gatgacaaaa gttattaatc
     1861 gttttaccat tttagtaggg gcattgattt taattttagc atccctattt ccaccgttag
     1921 gagcattctt taattcctta ccacaagcag ttttaggtgg atgtacagtg atgatgtttg
     1981 gttcaatcat gtatgaagga atcaaaatgc ttaaagaaaa tgagtttgat gagcgcacaa
     2041 tgatcattgt ttcattagct ttttgtattg gggtaggatt aactcaaact tcgggtaatt
     2101 tttttgcagc ttttccacca gtagtgggag atatatttaa tggtaatgct gttgcaggtg
     2161 tatttgttgt atctttgtta ttaagtttgg tgcttccgaa aaagaaagaa gcaaaaaagt
     2221 aaaaatgctt aaagaatcat aattgat
//
LOCUS       DAOMTA010000531         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2301_length_2246_cov_2.262894, whole genome shotgun sequence.
ACCESSION   DAOMTA010000531 DAOMTA010000000
VERSION     DAOMTA010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_2246_cov_2.262894"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2246
                     /locus_tag="PLK68_12520"
     CDS             <1..>2246
                     /locus_tag="PLK68_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HRM91269.1"
                     /translation="MNLSEIKINKTKYSHDVPGYISATDYCYGERIIVEDGNIGFFGE
                     YDRVNYRVNVQKAGTYQMKMNYASDGEAKFWMDLIQNGISASIGEATLSSTGNDSTYS
                     TGVVEINLPEGESQISFVPQSGVSFNLKALSFGSYIQISSDELEEGKLAGKEITVNLK
                     DGKFETDLNQANWVVEGLPAGITYSLKKINDTKAVIILKGNEVVDYDSNLTVTVKVDA
                     GEIGDTGYSLSDSVMIAAVDDDETLLDIGDLAFGTTEFDLMIEGGKFNQDLKVNDLVL
                     SEEAANYMTVKAVKVSADGNKVTVTLERKNTNYEDVAGTISIKSNGYSDGNVDLTANL
                     KLLKTNNLPDSIDVGDVAVGLKESDAYRKKGTLVNGAKGDYIDFYLNIKEEGNYVLTY
                     KIKDSEAITNGLKLSGGLGLATDNLGSVSFGKYWGNAQGYAQMLNLKAGEQTLRFEVN
                     SAGFELTNLKIEKLTQAIEVSDTLDQTTTITADKVVDGSKEIGWGIEGSTTKNIGFGS
                     AGAYQDYYLDVKTAGLYDVKVNYSHDCGGDTKAVIMTVDGKITATLGEVTLKNSGGWS
                     NWKDSDTIQIKLEAGKQFIRIYDDLDGFNYRNFQLTFKGEQDVTAPEITGNDAVIYVN
                     DATDIKDLLGLIVTDDVDGDLLDKVTISGEYNYNVAGTYTIAVTVSDEAGNETSKEFT
                     VKVVEPAKLIVEDKVLTVGDKFDPLAGIQVLDVDGTDITKNIIVISDGVDTSKAGSYE
                     VIYRITDA"
BASE COUNT          804 a          229 c          499 g          714 t
ORIGIN      
        1 atgaatctaa gtgaaattaa gatcaataag acaaaataca gccatgatgt gcctgggtac
       61 attagtgcta cagattactg ttacggtgaa agaattattg tagaagatgg aaatattggt
      121 ttctttggtg agtacgaccg agttaattat cgggttaatg tacaaaaagc tggcacttat
      181 caaatgaaga tgaactatgc tagtgatgga gaagcaaaat tttggatgga tttaattcaa
      241 aatggtatct ctgcttcaat tggtgaagca acactctcta gtactggtaa cgatagcact
      301 tacagtactg gggtagtaga gattaatctg cctgaaggtg aaagccaaat tagttttgtc
      361 ccacaaagtg gtgtaagttt taacttgaaa gcattatcgt ttggctcata tattcaaatt
      421 tctagtgatg aattagaaga aggtaaatta gctggtaaag aaattactgt taatttaaaa
      481 gatggaaaat ttgaaactga tttaaatcaa gctaattggg tggttgaagg attgcctgca
      541 ggtattactt attctttaaa gaaaataaat gatacaaaag cagtaattat attaaaaggt
      601 aatgaagttg tagattatga tagtaatctt acggtgactg ttaaagttga tgctggtgag
      661 attggtgata ctggttatag tttgagtgat agtgtaatga ttgctgcagt tgatgatgac
      721 gaaacattat tagatattgg tgatcttgct tttggaacaa ctgaatttga tttaatgatt
      781 gaaggcggaa aattcaatca agatttaaaa gtaaatgatc ttgttttaag tgaagaagca
      841 gctaattata tgactgttaa agcggtaaaa gtaagtgctg atggaaacaa agtaacagta
      901 acattagaac gtaaaaatac taattatgaa gatgttgcag ggacgattag tataaaatca
      961 aacggttata gcgatggcaa cgtcgattta acagcaaatc ttaaattatt aaaaacaaat
     1021 aatctaccag atagtattga tgttggtgat gttgcggtag gattaaaaga aagtgatgca
     1081 tatcgtaaaa aaggtacttt ggttaatggt gctaagggag attatattga cttctatttg
     1141 aatattaaag aagaaggaaa ttatgtattg acatataaga ttaaagatag tgaagcaatt
     1201 actaatggtt taaaactaag tggtggttta ggattggcta ctgataatct tggcagtgtt
     1261 tcttttggta aatattgggg aaatgcacaa ggttatgcac agatgttgaa tttaaaagct
     1321 ggtgaacaga ctttaagatt tgaagttaat agtgctggtt ttgaattaac taatttaaaa
     1381 atcgaaaaat taactcaagc aattgaagtt agtgatacgc ttgatcaaac aacgacgata
     1441 acggctgaca aggtagttga tggttctaaa gaaattggtt gggggattga aggttcaaca
     1501 actaaaaata ttggttttgg atctgcagga gcttatcaag attattatct tgatgtaaag
     1561 acggcaggat tatacgatgt aaaggttaat tatagtcatg attgcggtgg cgatactaaa
     1621 gctgttatca tgacagtaga tggaaaaata actgctactt tgggtgaagt tactttaaag
     1681 aattctggtg gctggtcaaa ttggaaagat agtgacacga ttcaaattaa attagaagct
     1741 ggtaaacaat ttattcgtat ttatgatgat ctagacggct ttaattatcg taatttccaa
     1801 ttaacattta aaggggaaca ggatgtcact gctccagaaa ttacgggtaa tgatgcagtt
     1861 atctatgtaa atgatgcaac agatattaaa gatttactag ggttaatagt tactgatgat
     1921 gttgatggtg atttattaga taaagtaaca attagtggcg aatataatta caatgttgca
     1981 ggaacttata cgattgcagt gactgtaagt gatgaagctg gtaatgaaac aagtaaagaa
     2041 tttacagtta aagtagttga acctgcaaaa ttaattgttg aagataaagt attaacagtt
     2101 ggcgataaat ttgatccatt agctggtata caagttttag atgttgatgg tacggatatt
     2161 actaaaaata ttattgtaat tagtgatggt gttgatacaa gtaaagcggg aagttatgaa
     2221 gttatttaca gaataactga tgcttt
//
LOCUS       DAOMTA010000532         2241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2310_length_2241_cov_2.203111, whole genome shotgun sequence.
ACCESSION   DAOMTA010000532 DAOMTA010000000
VERSION     DAOMTA010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_2241_cov_2.203111"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLK68_12525"
     CDS             complement(<1..236)
                     /locus_tag="PLK68_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HRM91270.1"
                     /translation="MEKKISSEIIYDGKIITVYKDKVECENGNLATREVVRHHGGVGV
                     LAIVDGKILLVKQYRYPNAVDTLEIPAGKLELNE"
     gene            452..1420
                     /locus_tag="PLK68_12530"
     CDS             452..1420
                     /locus_tag="PLK68_12530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM91271.1"
                     /translation="MKKLKITENDANQRIDKYLKKLLVNAPNNFIYKMFRKKDIKVNG
                     KKVDEKYILSLDDEVEMFLYDDKFKEFTETKSIYEVNKTFSVLYEDKHVLIVFKPAGL
                     LVHEDANESINTLTNQVLSYLASKNELDLSRENTFMPGPVHRLDRNTSGIVIFGKTLA
                     ALQNLNEMIKQRHCIEKKYLTICRGQLSHQRNLHGYMVKLENQAQVKLVKKDYPGALT
                     METIVRPLKYNCDYSLVEVTLITGRMHQIRVHLSSIEHPIIGDRKYGDFELNKYIKKT
                     FGLNNQLLHAYKIKFVKTFGVLNYLQDKEIICPVPALFEKIEKSLL"
     gene            1483..2241
                     /locus_tag="PLK68_12535"
                     /pseudo
     CDS             1483..2241
                     /locus_tag="PLK68_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538100.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT          799 a          269 c          435 g          738 t
ORIGIN      
        1 tcttcattta attctaattt acccgcagga atttctaaag tatctacagc attaggatac
       61 cgatattgct ttactaataa aattttgcca tcaacaatag caagaacgcc aactccgccg
      121 tggtgtcgaa ctacttctcg agttgcgaga ttcccatttt cacattctac tttgtcttta
      181 taaacagtga taatttttcc atcatagatt atttctgatg atattttctt ttccattagt
      241 ttcacctcat taaaagtata acaaaaaaag tatgcggggg tgcatacttt tttctattat
      301 ttcacgtaag ggtgataata gggggtatgt aaagcgaaat acttcactta acaaaatcaa
      361 ttatagcatc agtttcataa aatgtaaagg gttacaataa aagtattagg cgtttttagc
      421 aaattttgtt ataatgtttc aaggtgatat aatgaaaaaa ttaaaaataa cagaaaatga
      481 tgctaatcaa agaattgata aatatttaaa aaagttacta gttaatgctc caaataattt
      541 tatatataaa atgtttcgaa aaaaagatat caaagtaaat gggaaaaaag tggatgaaaa
      601 atatatttta tcattagatg atgaggtcga aatgttttta tatgatgata aattcaaaga
      661 atttacagaa accaaaagta tctatgaggt taataaaacg tttagtgttc tttatgaaga
      721 taaacatgta ttgattgttt ttaaaccagc agggctttta gttcatgagg atgcaaatga
      781 aagcattaat actttaacaa atcaggtttt aagttatctt gcttcaaaaa atgaactaga
      841 tctaagtcga gaaaatactt ttatgccagg accagttcat cgtttagata ggaatactag
      901 tggaattgta atttttggta aaactttggc agctttgcaa aatctcaatg aaatgataaa
      961 gcaacgtcat tgcattgaaa aaaaatattt aacgatctgt cgcggacaat tgagtcatca
     1021 gcgaaattta cacggatata tggtcaagtt agagaatcag gctcaagtta aattagtaaa
     1081 gaaagactat cctggggctt taacgatgga aacgattgtt aggccgttaa aatataattg
     1141 tgattatagt cttgttgagg ttacattaat aactggacgg atgcaccaga tcagagtaca
     1201 tttatcaagt attgaacacc caattattgg tgatcgtaaa tatggagatt ttgaactgaa
     1261 taaatatatt aagaagactt ttggtttaaa taatcaattg ctgcatgctt ataaaataaa
     1321 atttgtaaaa acctttggtg tactaaatta tcttcaagat aaagagataa tttgccctgt
     1381 gccggctttg tttgaaaaaa tagagaaaag tttgttatag acacctttaa ataaagagtt
     1441 gtttattgta taattattta catagattat aggaggaaag atatgaaata ctatattgga
     1501 attgatttag gtgggactaa tgttcgtact ttgctggtgg atgaaaatgg taaaacttat
     1561 agtgaagtaa aagataatac tgaaagagaa aatggtcctg actatgtatg tgctaaaatc
     1621 attcgtcaaa ttgaaagttt agatacatca atttgtggtg gtttacaagg tgtaagtgga
     1681 attggtattg gagtacctgg acctgttgac acagtaaaag gtacaatgat catggcaaca
     1741 aatttacctg gattcgaaaa ttatccaatt tgtgataaat tagcagatcg ttttaattta
     1801 ccaactttta ttgataacga tgcaaatgtt gctggattag cagaagcttt attaggtgct
     1861 ggaaaagatt atccgacatg ttatttcaac aggaattggg ggagccttta ttgttgatgg
     1921 taaattagtt tctggtggtc gaggacatgc tggagaaatt gggaatatta ttgttaaaaa
     1981 caatggttat aaattcggag gtttaaatcc aggggctgct gagggagaaa ctagtggtac
     2041 tgcaattact cgtaaaggta aagaactttt aggagaagat agagtaaatc atgctggaga
     2101 tgtttttaga ttggctagcg aaggtgattt aaaagcacaa agtatagttg atgaatgtat
     2161 ttcagaatta gcaacaatgt ttgctaatat tgctcacact gttgatccgc actgttttgt
     2221 aattggtggt ggtgtaatga a
//
LOCUS       DAOMTA010000533         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2315_length_2239_cov_2.159799, whole genome shotgun sequence.
ACCESSION   DAOMTA010000533 DAOMTA010000000
VERSION     DAOMTA010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_2239_cov_2.159799"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLK68_12540"
     CDS             complement(<1..519)
                     /locus_tag="PLK68_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HRM91272.1"
                     /translation="MNASSDAAEQIVRISLNGMEVAAKTTGKAAVEVANMLYAVMKDQ
                     KKTKGKTRLENLISTGKPLSVFCLKSEDFTCFQKEAKRYGVLYYAVRTQRSDTDNVVD
                     IMVKLEDASRMNRIIERFGLTDTSQSAQVKTDIVRTRANKQKNEKEIIRPTKSAKTLE
                     QEEQNKAVLNPEK"
     gene            complement(494..1903)
                     /locus_tag="PLK68_12545"
     CDS             complement(494..1903)
                     /locus_tag="PLK68_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HRM91273.1"
                     /translation="MATTSIWKVEGWLGKVLIYAENPEKTDNPAYYQSEKLSQEEKQG
                     LTDVIQYAMREEATSSMADKERFVSGVNCSPNTAREEMLAVKKRYGKNEGIVAFHGYQ
                     SFAPNEVTPEVAHEIGVKLANELWGDRFQVIVATHLDKQNHLHNHFVLNSVSYIDGYR
                     YSDCRATYKLMRDTSDRLCKEYQLSVIENPAKNKSKHYAEWRAENEGVLTWRGIIKND
                     LDEAIVSSVTDKQFFHKLREKGYYIKQGKDITVRPQGKQRGMKIARNFGEDYTYEAIC
                     QRIMQNPIPAQRKPTITKRTVTVLRLHGDLSKQRKIGGLRGRYLYYCYKLGIFPKNKP
                     ISDARIHFLLKEDIRRMHELSQEAKLLCTNKIDTTEQLFSYQSKKKEEMQQHIQTRKH
                     LRYRLRRKTLSDEDKQGLKYEIAELSKQIDILRKEVILCDDIAERSQVIKDKLDIIRQ
                     DETENRKEEKADERKFRRS"
     gene            complement(1906..>2239)
                     /gene="mobC"
                     /locus_tag="PLK68_12550"
     CDS             complement(1906..>2239)
                     /gene="mobC"
                     /locus_tag="PLK68_12550"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HRM91274.1"
                     /translation="NKMRKRNIAVITRLNKQEQQHLKVMVKRSGLSQEAYIRHLINGV
                     IPNDAPSPDYYAMMKELHAIGNNLNQIASKAHRLNVIDVQEYDKAVRLFEKTVKDITN
                     AVITPRKY"
BASE COUNT          610 a          436 c          339 g          854 t
ORIGIN      
        1 cttttccgga ttcagtactg ctttattttg ttcctcttgt tcaagtgttt ttgcagactt
       61 agtaggacga ataatttctt tttcattttt ttgtttattt gccctagtac gtacaatatc
      121 tgttttgacc tgtgctgatt gacttgtatc tgttagacca aaacgttcaa taatacgatt
      181 catacgtgat gcatcttcca gcttaaccat aatatcaaca acattatcag tatcagaacg
      241 ttgagtacga actgcatagt acaaaacacc gtaacgcttt gcttcttttt gaaaacaagt
      301 aaaatcttct gatttcaaac aaaatacaga taatggcttt cctgtactga ttaagttttc
      361 tagtcttgtt ttaccttttg ttttcttttg atctttcatc actgcataaa gcatattggc
      421 aacttcaaca gcggctttac cagtagtttt agcagcgact tccataccat ttaatgaaat
      481 acgaacaatt tgttcagctg cgtctgaact tgcgttcatc ggctttttcc tcctttctgt
      541 tttcggtttc atcttgccga ataatatcta atttatcttt tatcacttgt gaacgctcgg
      601 caatatcgtc acagagtatc acctcctttc gtaaaatatc aatctgttta gaaagttcag
      661 caatttcata tttaagacct tgcttatctt catcacttaa tgtttttctt ctcaagcgat
      721 aacgaagatg ttttcgtgtc tgaatatgct gctgcatttc ttctttcttt tttgattgat
      781 atgaaaaaag ctgctcagtt gtatcaatct tgttggtaca tagcagcttt gcttcttgac
      841 ttaattcatg catacgtcgt atatcttcct ttaataaaaa atgtatcctt gcatcagaga
      901 taggtttatt tttaggaaag atacctaatt tataacagta atataaataa cgacctctta
      961 atccaccaat cttacgctgt ttcgatagat caccatgtaa tcgtaaaact gtaacagttc
     1021 ttttcgttat agtaggtttt ctttgagctg gtataggatt ttgcataatt ctttggcata
     1081 ttgcttcata ggtataatct tcaccaaaat ttctagcaat cttcattcct ctttgttttc
     1141 cttgtggtcg aactgtgatg tctttacctt gtttgatata atatcctttc tctcgcagtt
     1201 tgtggaaaaa ttgcttgtcc gttacagaag aaacaattgc ctcatctaaa tcatttttta
     1261 taattcctcg ccatgttaag acaccctcat tttctgctct ccactctgca taatgttttg
     1321 acttgttctt tgcgggattt tcaataacag ataactggta ttctttacat aggcgatctg
     1381 aagtatctct catcagttta taagttgctc tgcaatcgct atagcggtat ccatctatat
     1441 aggaaacaga atttaaaaca aaatggttat gcaaatgatt ttgcttatcc aaatgagtag
     1501 caactataac ttgaaatctg tcgccccata attcattggc tagtttaaca ccaatttcat
     1561 gagcaacttc tggagtcacc tcattaggtg caaaagattg atacccatga aatgcaacga
     1621 taccttcatt tttgccataa cgttttttta ctgctagcat ttcctctctt gcggtattag
     1681 gagaacaatt tacaccactg acaaaacgtt ctttatcagc catagaactg gtagcttctt
     1741 ctcgcattgc atactggata acatcagtta gtccctgttt ttcttcttga gataattttt
     1801 cactttgata ataggcagga ttatctgttt tttcaggatt ttcagcatag attaacactt
     1861 tacctaacca gccctcaact ttccaaattg atgttgtagc catatctaat atttacgtgg
     1921 tgtaattact gcattagtaa tatctttaac tgtcttttca aatagacgaa ctgccttatc
     1981 atattcttga acatcaatga catttaaacg gtgagcctta gatgcaatct gattaagatt
     2041 attaccaatc gcatgtagtt ctttcatcat ggcatagtaa tctggtgaag gtgcatcatt
     2101 agggattact ccgttaatca aatgacgaat atacgcctct tgtgataaac ctgaacgttt
     2161 gaccatcact tttaagtgtt gttgttcctg tttgtttaat ctagtgataa ctgcaatgtt
     2221 tctttttctc attttattt
//
LOCUS       DAOMTA010000534         2239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2316_length_2239_cov_1.997253, whole genome shotgun sequence.
ACCESSION   DAOMTA010000534 DAOMTA010000000
VERSION     DAOMTA010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2316_length_2239_cov_1.997253"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLK68_12555"
     CDS             complement(<1..730)
                     /locus_tag="PLK68_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRM91275.1"
                     /translation="MNVEIINVGTELLLGEIVNTNATYIQKMCKDLGFNVYYQTTVGD
                     NHDRFKECLDVAFKRGANCVITTGGLGPTQDDLTKELSAEYLGLELLYNEEEAKKVEE
                     KCRFVTGWGDIPENNFKQAFFPKDCYILENEVGTANGCVMSKDEKIIVNLPGPPKEMT
                     YVVDHVLKDYLSQFKQDIIYTYDFLTMGIGESRVDEVLADLIDHQEEVSIALYASEET
                     VRVRLGCKASDKETADKKIAPIKTE"
     gene            complement(971..1126)
                     /locus_tag="PLK68_12560"
     CDS             complement(971..1126)
                     /locus_tag="PLK68_12560"
                     /inference="COORDINATES: protein motif:HMM:NF040063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HRM91276.1"
                     /translation="MRIATIIDKGLLEFKSTIIKDFILAFCDSQFIAKYDYLEMLSQL
                     IEIFYYY"
     gene            complement(1615..>2239)
                     /locus_tag="PLK68_12565"
     CDS             complement(1615..>2239)
                     /locus_tag="PLK68_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRM91277.1"
                     /translation="KILEVLFEYYPTQTIYWFIGLIAFSIPLFLKGEMKGEKLVIIPF
                     ICGLAIIFGLEFLNPGEGKVVVNPDFPALSAGLFVKMIIIGAVSGATMIMPGVSGSMV
                     LLILGEYYLFKSYLANVTSFSLDVIMPLGFMAIGIAVGIVVSAKLCSYFTKTHKAGFL
                     SLILGLIVASSLVLIPFDVSYNLSLVVTSIIAVIFGGIIVLGLSKIQ"
BASE COUNT          760 a          474 c          244 g          761 t
ORIGIN      
        1 tttctgtctt aattggagca atttttttat cagcagtttc tttatcgctt gctttacaac
       61 ctaatcgaac ccgcactgtt tcttctgaag cataaagagc aatactaact tcttcttggt
      121 gatcaattaa atctgctaag acctcatcaa cccgactttc tcctattccc atggttagaa
      181 aatcatatgt gtaaataata tcctgcttaa attgacttaa ataatctttt aaaacatgat
      241 caacaacata agtcatttct ttaggcggac caggcaaatt aacaattatc ttttcatctt
      301 tcgacattac acagccattt gctgtcccta cttcattttc taaaatataa caatctttag
      361 gaaaaaaagc ttgtttaaaa ttattttccg gaatatcacc ccatcccgtt acaaatcgac
      421 atttttcttc taccttttta gcttcttctt cattatataa taattctaaa cccaaatatt
      481 ctgccgataa ttcttttgtt aaatcatctt gggtaggtcc taatccccca gtcgtaatta
      541 cacaattagc accacgttta aaagctacat ctagacattc tttaaaacga tcatgattat
      601 caccaactgt cgtttgatag taaacattaa accccaagtc tttacacatt ttttgaatat
      661 aagttgcatt agtattaaca atttctccta ataacaattc tgtcccaaca ttaattattt
      721 caacattcat gaaattatcc tccttaatga ttaactatat tatagttaat tttaatcatt
      781 ttgctagtct tttaatcatt aaaattattt cttgcatatt ttccttctag cacctaagac
      841 caaaggtcta gttgaatacc aaaataaact aacaaaaatg actaaacaag caataaaagt
      901 taattattag ctgaattatt cctaaatcga attcaatata catatcactc actccctcgt
      961 caacttcact tcaatagtaa taaaaaattt ctatcagctg acttagcatc tctaaataat
     1021 cgtatttggc aataaactga ctatcacaaa atgctaaaat aaaatctttg ataatagtac
     1081 ttttgaattc taataacccc ttatcaataa tagttgctat cctcactttc ggactatcca
     1141 aatattactt atagcgattt acactctata aacgtttcag tccttcatca tttctgacta
     1201 ctatgacctc atctgatttc ttgtgataaa tctttttcta tcgatggtat cattactgct
     1261 tttaaagttt actggacttc atcgtccaca aggtctcccg aggtaaggca tacatctttt
     1321 tttatcttca caggtttttg atttactgtc ttggttttac gtttaccttt tgaacttggt
     1381 ttgttctgca accttgtcca ccattcagcc tcatcaaatt tcttttcgta acctcgaaga
     1441 ttttgccaca ccacttcctc cgcccatagc atcactacta acggcttgtg gttcactact
     1501 cttggcggta aatacccgtg gttggacttt caccagctag atgtatgcca tgctcgacac
     1561 acagaaaaag aagtcattgg ttttacaatc aataacttct tttttgtatt ttaactattg
     1621 aatcttagat aatccaagaa caatgattcc tccaaaaata actgcaataa ttgatgtcac
     1681 aactaaactt aaattataac ttacatcaaa aggaatcaat actaatgacg aagcaacgat
     1741 caatcctaaa atcaaactca agaatccagc tttatgagtt ttagtgaaat atgaacataa
     1801 ttttgcacta acaactattc ctacagcaat accaattgcc ataaatccta gcggcataat
     1861 aacatccaga ctaaaactag taacatttgc tagataagac ttaaatagat aatattctcc
     1921 taaaattaat aaaaccatac taccgctaac accgggcata atcatagttg ctcctgatac
     1981 ggcaccaata attatcattt taacaaacaa accagcactt agagcaggaa aatcaggatt
     2041 aacaactact ttcccttctc caggatttaa aaattctaac ccaaagataa ttgctaaacc
     2101 acaaataaaa gggataatta caagtttttc acctttcatt tcccctttta aaaataaagg
     2161 aatactaaaa gcaatcaaac caataaacca ataaatcgtc tgcgttggat aatattcaaa
     2221 taacacttcc aatattttc
//
LOCUS       DAOMTA010000535         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2339_length_2228_cov_2.505752, whole genome shotgun sequence.
ACCESSION   DAOMTA010000535 DAOMTA010000000
VERSION     DAOMTA010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2339_length_2228_cov_2.505752"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..815
                     /locus_tag="PLK68_12570"
     CDS             144..815
                     /locus_tag="PLK68_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRM91278.1"
                     /translation="MDKNELNDEVEIDLSQLLKLLKKIIILALVGIIIAASATTFLIS
                     KKYQSQGSVLLKADVVNGSLDSTQVNTNKMMVNNYVKLLQGNNIQDQVAKNLNITSAE
                     VRSSLSITNTTDTQIIEISSTTVDPGLSKRIVDETISVFTTLIQEKLDVTNVTIVDQP
                     EVNPNPVSPSMVKNVIIGAVAGIVISLGYLLLTYLLDTKIKNGEQAEQYLGVPLLGIV
                     PFFEE"
     gene            818..1594
                     /locus_tag="PLK68_12575"
     CDS             818..1594
                     /locus_tag="PLK68_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91279.1"
                     /translation="MAFIDIHSHYAWNVDDGIETREDAQAALKKAQIQNITKIVATPH
                     LTPGTTTAKEFEQIKERIEELKKLAKEYQIEIYPGCEVMLNGDYLELLDNHQYLTINN
                     GPYLLVEFNVTQKLPEDYDDRLYEYGIKQKIVIAHVERYFHHSIDLNLIQEWIDRGYI
                     IQVNSTSLLGIHGSMIQENAYQLLDNGMVHVIANDVHRPNGKRSVNLQETYEVLAKKY
                     QAEDLTRLMYDNPLAIINGHAPELIKVRKISKLPWFKRRK"
     gene            1595..>2228
                     /locus_tag="PLK68_12580"
     CDS             1595..>2228
                     /locus_tag="PLK68_12580"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRM91280.1"
                     /translation="MFKKNKKQSEKDTYLITSPDKKHGKIDFKEVYRHIRTNIEYSTV
                     GKNVKAINVTSSIPSESKSTTSLNLAMIYATKYANVLLIDADLRKATQHRYLKLSNSR
                     GLTNALIEYGETKKISSKCFQFIEDESFVGKLSVLTSGVKVPNPSELLSSQIFEDFIN
                     ELKGLYDFIIIDCPPIMLVSDAIPIGNVVDGTIFVCSSQLTGRKDAKASIE"
BASE COUNT          830 a          324 c          360 g          714 t
ORIGIN      
        1 aacaactctg tcacacattc tattttaaca tttttctaca ataaatatta atttttattg
       61 gtaatttctt gcattttatc cgcttataaa ggataatacg ttttggaata gttttattca
      121 atatattaat aatgggagga cttatggata aaaatgaatt aaatgatgaa gttgaaatcg
      181 acttatcaca attattgaaa ttactgaaaa agattattat cttagcctta gtcggaataa
      241 ttattgcagc ttctgcaact acctttctaa taagtaaaaa ataccagtca caaggaagtg
      301 tattattaaa agctgatgta gttaatggat cattagactc aactcaagtt aatacaaata
      361 aaatgatggt aaataactat gttaaattat tacaaggtaa taacattcaa gatcaagttg
      421 ctaaaaactt aaatattact tctgccgaag tacgcagttc attaagtatt acaaatacta
      481 ctgatacaca aattattgaa atttcttcaa caactgttga tcctggactt tctaaaagaa
      541 tcgttgatga aacaattagt gtttttacaa ctttaatcca agaaaagcta gatgtgacaa
      601 atgttacgat cgttgatcag cctgaagtaa atcctaatcc agtttcacca agcatggtta
      661 aaaatgttat tattggggca gtagccggta tcgttattag tttggggtat ttattactta
      721 catacctatt agatactaaa attaaaaacg gtgaacaagc tgaacaatat ctaggggtcc
      781 ctctattggg aatcgtccct ttctttgagg aataaccatg gcatttattg atatacatag
      841 tcattatgct tggaatgttg atgatggtat cgaaactcga gaagatgcac aggcagcatt
      901 aaaaaaagct caaattcaaa atattactaa aattgtagca actcctcatc ttactccagg
      961 aacaactaca gcaaaagagt ttgaacaaat taaagaacgg attgaggaat taaaaaagct
     1021 tgccaaagaa tatcaaattg aaatctatcc aggttgtgaa gttatgctta atggagacta
     1081 tctagaacta ttagataatc atcaatattt aactattaac aatggaccgt atttacttgt
     1141 tgaatttaat gttactcaaa aattacccga agattatgat gatcgtctat atgaatatgg
     1201 aataaagcaa aaaattgtta tcgctcatgt agaacgttat tttcatcata gtattgattt
     1261 aaatctaatt caagagtgga ttgatcgtgg ctatattatt caagttaaca gcactagttt
     1321 attaggaatc catggtagta tgattcaaga aaatgcatac cagctattag ataatggtat
     1381 ggttcatgta atcgctaatg atgttcatcg ccctaatggt aaacgtagtg ttaatctcca
     1441 agagacatat gaagttttag caaaaaaata tcaagctgag gatttaacta gactcatgta
     1501 tgataaccct ctagccatta tcaatggaca tgcgcctgaa ttaataaaag ttcgtaaaat
     1561 atctaaatta ccttggttta aaaggagaaa ataaatgttt aaaaagaata aaaaacagag
     1621 tgaaaaagac acatatctga ttacttcgcc agataaaaag catgggaaaa tcgattttaa
     1681 agaagtatac cgccacatta gaacaaacat cgaatattct actgttggta aaaatgttaa
     1741 agcaatcaat gtgacatctt caattcctag tgaatcaaaa agtactacat cattaaactt
     1801 agcgatgata tatgcaacga aatatgcaaa tgtcttattg attgatgctg atttaagaaa
     1861 agctacccag catcgttatt taaaactatc gaactcacga ggattaacta acgctttgat
     1921 cgaatatggt gaaactaaaa aaattagttc aaaatgtttc caattcattg aagatgaatc
     1981 gtttgtagga aaattatcag ttttaacttc tggggtcaaa gtaccaaacc ctagtgaatt
     2041 attaagtagt caaatcttcg aagactttat taatgaatta aaaggattat acgattttat
     2101 tatcattgat tgtcctccaa ttatgttagt tagtgatgct attccgattg gtaatgtggt
     2161 tgatggaaca atctttgttt gctcatcaca actaactggt cgtaaagatg ctaaggcttc
     2221 aatcgaaa
//
LOCUS       DAOMTA010000536         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2348_length_2217_cov_2.078631, whole genome shotgun sequence.
ACCESSION   DAOMTA010000536 DAOMTA010000000
VERSION     DAOMTA010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_2217_cov_2.078631"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1390
                     /locus_tag="PLK68_12585"
     CDS             71..1390
                     /locus_tag="PLK68_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRM91281.1"
                     /translation="MDKQISQELVTFIKQSPTAFHAVANMQNILIEHGYEELLEGQTW
                     QIKKGGHYFVTRNNSSIIAFNLGENLDNYSFNVAASHSDSPTFKVKENAEIEIKGKYT
                     QLNTEGYGGMLCATWFDRPLSIAGRVLVQEGDNYVTKLVNIDRDLVMIPNVAIHMNRT
                     VNDGYAYNKQVDMLPLFGGSETKAGDLKKLIADELGVDVETIYGTDLYLYNRMEPSIW
                     GANEEFISCPQLDDLQCAYTSLQGFLKGANKQSINVFACFDNEEVGSGTKQGAGSTFL
                     YDAMRRINNALGKGEEEYYRALASSFMLSADNAHAVHPNHPAKTDVNNCVYMNEGIVV
                     KSHAGQKYTSDAVSIAVFKGLCKKAGVPVQFFSNRSDTAGGSTLGNIAMAQVSMNSVD
                     IGLPQLAMHSSYETAGVKDTAYMIKVMEEFFNSHIEEMSGHILKVTK"
     gene            1390..1749
                     /locus_tag="PLK68_12590"
     CDS             1390..1749
                     /locus_tag="PLK68_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91282.1"
                     /translation="MKKEKTSKKRLKEIKKEVLEKYIIAGLWQTMCGYIVSFSVDVLI
                     AIIAFYVTLHNLVNQYKLIKENRLSLKPFSFQIFGIIVGLFIVILTLKSPFDISFAIL
                     VIAFLTSKKMFEKELMK"
     gene            complement(1855..>2217)
                     /locus_tag="PLK68_12595"
     CDS             complement(1855..>2217)
                     /locus_tag="PLK68_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91283.1"
                     /translation="REIIMNTKKINEQAIESLKTYFNEGKDLIKENVTPLTKDQIKAI
                     VNEEIAHHEAKLHELNDLKQKIDLEAEEAFEKVFGKAKEEPKTHKLTTDEMAVKLQEE
                     KEHQMDVANKLKEEKLSH"
BASE COUNT          733 a          277 c          396 g          811 t
ORIGIN      
        1 cttagacata tcctttaaca catgttaaag gatatgttac aataaagccg taattatgga
       61 ggtcatgaaa atggataaac aaatatcaca ggaattagta acttttatta agcaaagccc
      121 aactgctttt catgctgtag ctaatatgca gaacatttta attgaacatg ggtatgaaga
      181 attattagaa ggacaaacgt ggcaaattaa gaagggtggt cattactttg tcactagaaa
      241 taattctagc attattgctt ttaatcttgg tgagaattta gataattata gttttaatgt
      301 tgctgcatca catagtgatt caccaacttt taaagtcaaa gagaatgctg aaattgaaat
      361 aaaaggtaag tatacgcaat taaatacaga gggttatggt gggatgttat gtgctacctg
      421 gtttgatcgc ccattatcta ttgcaggtag ggttttagtt caagaaggtg ataactatgt
      481 taccaaacta gtaaatattg atcgagatct agtaatgatt ccgaatgttg caattcacat
      541 gaatcgtact gtcaatgatg gttatgctta taataaacaa gttgatatgt taccgttatt
      601 tggtggatct gaaactaaag caggagattt aaagaaactg attgctgatg aattaggtgt
      661 tgatgttgaa actatttatg gaacagatct atatttatat aatcgcatgg aaccatcaat
      721 ttggggagct aatgaagaat ttatttcatg tccacaacta gatgatttac aatgtgcata
      781 tacttcatta caaggttttc tcaagggagc aaataagcaa tcaatcaatg tatttgcctg
      841 ctttgataac gaagaagtag gttcaggtac taaacagggt gcaggatcaa catttttata
      901 tgatgctatg cgtagaatca acaatgcttt aggaaaaggt gaagaagaat attatcgggc
      961 attggcttca agctttatgt taagtgctga taatgctcat gctgtacacc caaatcatcc
     1021 tgcgaaaaca gatgttaata attgtgttta tatgaatgag ggtattgttg taaaatctca
     1081 tgctggtcaa aaatatacta gtgatgcagt tagtatagct gtttttaagg gattatgtaa
     1141 aaaggctgga gttccagtac aatttttctc taatcgcagt gatactgctg gtggtagcac
     1201 gttaggtaat attgcaatgg cacaagtttc aatgaacagt gttgatatag gattacctca
     1261 attagcaatg cattcatctt atgaaacagc tggtgtcaaa gatactgctt atatgattaa
     1321 agtgatggaa gaattcttta atagtcatat tgaagaaatg agtggacata ttttaaaggt
     1381 tactaaataa tgaaaaaaga aaaaactagt aaaaagcgat taaaagagat aaaaaaagaa
     1441 gtacttgaaa agtatattat tgctggttta tggcaaacga tgtgtggcta tattgtcagt
     1501 ttttctgttg atgttttgat tgcgattatt gctttctatg ttacattaca taatctagtt
     1561 aatcaataca aactaattaa ggaaaatcgg ttgtcattaa agcctttctc ttttcaaatt
     1621 tttggaatta ttgttggttt gttcattgta atacttactt taaagagtcc ttttgatatt
     1681 tcttttgcga ttcttgtaat tgctttttta acaagtaaaa aaatgtttga aaaagaacta
     1741 atgaaataac tgtacgtttg tatggctatt ttgataacta ctttttataa tgttatcaga
     1801 ttaataaaaa agtcacgata ttgatttgtc gtgacttttt ttagaataat taatttaatg
     1861 agataatttt tcttctttta atttattagc aacatccatt tgatgttctt tttcttcttg
     1921 aagtttgact gccatttcat cagtagttag tttatgtgtt tttggttctt ctttagcttt
     1981 accaaaaact ttttcaaaag cttcttctgc ttctaaatca attttttgtt ttaaatcatt
     2041 taattcatgt aatttagctt catgatgagc aatttcttca ttaacgatag ctttaatttg
     2101 atctttagta agtggtgtaa cattttcttt aataagatct ttaccttcgt taaaataagt
     2161 ttttaaagat tcaatagctt gttcattgat ttttttagta ttcataataa tttccct
//
LOCUS       DAOMTA010000537         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2352_length_2214_cov_2.309866, whole genome shotgun sequence.
ACCESSION   DAOMTA010000537 DAOMTA010000000
VERSION     DAOMTA010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2352_length_2214_cov_2.309866"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..520
                     /locus_tag="PLK68_12600"
     CDS             128..520
                     /locus_tag="PLK68_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91284.1"
                     /translation="MKYKEFRMLIFSIIFFFVIGGVCVYFALDHNRSKVIIGVILSFV
                     VLMMLTTKYNMKIFNDSMLVYEFKGIGILPALIDYKDVKEIKLVSKHKVKIKHKGIST
                     LYILDAESFYEEVMENIEEYKKSVNQED"
     gene            complement(538..1873)
                     /gene="gdhA"
                     /locus_tag="PLK68_12605"
                     /pseudo
     CDS             complement(538..1873)
                     /gene="gdhA"
                     /locus_tag="PLK68_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
BASE COUNT          730 a          374 c          318 g          792 t
ORIGIN      
        1 acatgccttt cacgcatgca ttcacgggtt cgaatcccgt acaggtcacc atttgaaatt
       61 agcaaccttt gggttgtttt tatttttgat tatgatatac tattgaatat taataagagg
      121 tggattcatg aaatataaag agtttagaat gttaattttt tcaattatct ttttctttgt
      181 gattggcggt gtgtgtgttt attttgcact agatcataat cgttcaaaag tgattattgg
      241 tgtaatctta tcgtttgttg tattaatgat gttaacaaca aaatataata tgaagatatt
      301 taatgattcg atgctagttt atgaatttaa aggaattgga atcttaccag ctttgataga
      361 ctataaagat gttaaagaaa taaaattagt gagcaaacac aaagttaaga taaaacataa
      421 aggtatttca acactttata ttttagatgc tgagtcattt tatgaagaag taatggaaaa
      481 tatcgaagaa tataaaaaaa gcgttaatca agaagattag cgcttttatc ttatctatta
      541 ataaattcct tgagaaatca ttgctgcaac aactttttca aatccggcaa tgtttgcacc
      601 ttttacaaga tcatatccaa gaccgtattt ttcagccgca gcttttgaat gatcgtgaat
      661 gtctttcata atttgtttta atttagcatc aacttcttca cgtgtccaat tataacgcat
      721 tgagttttga gccatttcta atgcagaaac tgcaacccca ccagcattag cagcttttgc
      781 aggtcctaga gttccaccgt tactaataaa gtattccatt gcttcaggtg tagtaggcat
      841 attagcacct tcaataatgt atttaacacc attagcagtt aattgtttag cttcttctat
      901 accaatttca ttttgtgtag cgcatgggaa agccatatct acttttaatc cccaaggttt
      961 ttcaccagcg tggaattcac atccgaattt ttcagcatag tcttttagtt taacatcatt
     1021 tgattcacga attttacata agaaatcaat tttttcatct gtagtaattc cttcaggatc
     1081 atatacatat ccatcacgtc cagaaattga tacgacttta gctccaaatt cacgagcttt
     1141 taaacaaaca ccccatgcta cgtttccata tccagaacaa gcaattgttt ttccttcata
     1201 agaatcattg aaatgttcta atacttcttt accatagtag catgctccaa atccagttgc
     1261 ttcgggacga attaaacttc ctccatatgg caatggctta ccagttaaaa caccattttc
     1321 aaaagcacca cgaatacgac gatattgacc atacatataa ccaatttcac gggcacctgt
     1381 accaatatcc ccagcaggta catctacgtc tggtccaata tgacgatata attcagtcat
     1441 gaagctttgg cagaaacgca taatttcgtt atccttgtgg atcgaaatca gatccacctt
     1501 taccaccacc gataggtagt gtagttaatg catttttgaa aatttgttca aatcctaaga
     1561 atttgataat tccaagattt acactaggat gtaatcttaa tcccccttta tatggtccaa
     1621 ttgcacttga gaattgaaca cgatatccac ggtttacttg tacttgacca tcatcatcca
     1681 tccaaggtac tttaaacatt aaagttcttt caggttctac taatctttca agaatagctt
     1741 gtttttcata ttcaggatga gcttcgatta caggtcgtaa gctttctaat acttcttcaa
     1801 ctgcttgaat aaattcaggt tgatcagcat ttttttcttt tacttgatta aatactttag
     1861 taatataatc catttttata ttgtcctcct tcttgtatat ttaatataat actattagtc
     1921 tgataataaa taaatcattt aagaaaaaaa tattataaat aaatataatt attatattgc
     1981 ataaaaaata ccatctttat aatattgtct tttaaaatac tatttgtata tcaataaata
     2041 catagctgct ataaagagag tttccaatat agtgagctaa taagttaata aacttatttt
     2101 ttgtatactt agcgtgagga ttaaaaataa aatttattat ttgataataa tagatttttc
     2161 tttgtgaaaa agggcaaact atagtagaat aaacagtgga ttctttttag aaat
//
LOCUS       DAOMTA010000538         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2354_length_2213_cov_2.506487, whole genome shotgun sequence.
ACCESSION   DAOMTA010000538 DAOMTA010000000
VERSION     DAOMTA010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_2213_cov_2.506487"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..831)
                     /gene="pflA"
                     /locus_tag="PLK68_12610"
                     /pseudo
     CDS             complement(119..831)
                     /gene="pflA"
                     /locus_tag="PLK68_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
     gene            complement(887..>2213)
                     /locus_tag="PLK68_12615"
     CDS             complement(887..>2213)
                     /locus_tag="PLK68_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM91285.1"
                     /translation="VKFARIPSYNQLFSGDPVWATLEVAGLGTDGRSMVTKNDFRFLH
                     TLENMGPSPEPNLTVLYTAALPENFKKYAAKISIDTSSVQYENDDVMRPVWGDDYSIC
                     CCVSATQTGKEMQFFGARANLAKCLLYAINGGIDEKTKTQVAPKYRPITSEYLDYEEV
                     MERYDQMMEWLADIYVNTLNLIQYMHDKYYYEAAEMALIDTDVRRTFATGIAGFSHVV
                     DSLSAIKYAKVKTVRDEDGIAIDYEIEGDFPRYGNDDDRADDIAVWLLKEFLNKLKKH
                     HTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQSGLLASLN
                     SVAKLPYEWALDGISNTQTISPDTLGHDEEERKTNLVQVMDGYFAQGAHHLNVNVFGT
                     EKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQMDVISRTCHKSM"
BASE COUNT          713 a          393 c          324 g          783 t
ORIGIN      
        1 caaatgaata gtttaagtgc aaaaacagaa acttttgaca agatgtttca taagttttcg
       61 taaaatcatc aaattcatga aactacgttt catctaaaaa aatatagata gtattaaact
      121 atctatattt atccgtatgt aataatttat tagcgttatc aatacgctct tgatttggtg
      181 ggttaattcc gtctaattga taaggaatcc ctagttcttc ccatttaaat actcctagtg
      241 tatgataagg taaaacttcc actttcttaa cattattcaa agtactaata aatttatcta
      301 attctgttaa atattcatct ttatcactga tacccggtac taaaacatga cgaatccaaa
      361 caggtttatt tatcgtactt aaatattttg ccatatctaa aatattatca ttaccttttc
      421 ctgttaactc ttgatgctgt gtactatcaa tatgtttaag gtctactaat aataaatctg
      481 tatatttcat taattcatta aatttagaaa aaaacggttc ttcgctaatt taaataattc
      541 aattaaaaaa tcaatttgta ataatggttc accaccgcta actgtaattc caccatcatt
      601 tccccaatag ctcttgtatt taagtgcttt agcaattgct tcttgaggtg tcatttccat
      661 tgtcgactta gcattagccc aagtatctgg attatgacag aatttacagc gtaacgggca
      721 tccatgtaga aagattatat agcgtattcc cggaccatca actgaaccga aactttcaat
      781 agaatgtaca aaacccttga ttgctgtttc catgttaatc tcctttttca ttaaaacagc
      841 ctcttttaat taagaggctg ttgttatgct tatttttcaa taactattac atagatttat
      901 ggcaagttct tgaaataaca tccatttgtt gttcacgagt taaatcaatg aatttaactg
      961 catatccaga tacacgaatt gtgaagtttg catattcttc tttttctgga tgttccatag
     1021 catcaattaa tttttcagtt ccaaatacgt ttacatttaa gtgatgagca ccttgtgcaa
     1081 agtatccatc cattacttga actaaattag ttttacgttc ttcttcatca tgacctaaag
     1141 tatcagggct gattgtttga gtattagaaa taccatctaa tgcccattcg tatggtaatt
     1201 tagcaacaga atttaatgaa gctaataaac cagattgttc tgcaccgtaa gaagggttag
     1261 cacctggtga taatggttca ccagctttac gtccatcagg taatgaacca gtagctttac
     1321 cataaacaac attagaagta attgttaaga tagaagtagt tggttctgaa tcacggtaag
     1381 tgtgatgttt tttaagttta tttaagaatt cttttaataa ccatactgca atatcatcag
     1441 cacgatcatc atcgttacca taacgaggga agtctccttc aatttcgtaa tcaattgcaa
     1501 taccatcttc atcacgaact gttttaactt tcgcatattt aattgcactt aatgaatcta
     1561 ctacatgaga gaatccagca ataccagttg caaatgtacg tcttacatct gtatcaatta
     1621 aagccatttc agctgcttca taataatatt tatcatgcat atattggatt aagttaagtg
     1681 tatttacata aatatcagct aaccattcca tcatttggtc atatctttcc attacttctt
     1741 cgtaatctaa atattcagaa gtgattggac gatattttgg agctacttga gtttttgttt
     1801 tttcatcgat accaccattg atagcgtata ataaacattt agctaagtta gcacgagctc
     1861 cgaagaattg catttcttta ccagtttgag ttgcagatac acaacagcaa attgaataat
     1921 catcacccca tacaggacgc attacatcat cattttcata ttgtactgag ctagtatcaa
     1981 ttgaaatctt agctgcatat tttttgaaat tttctggtaa tgcagcagta tataatactg
     2041 ttaagtttgg ttctggtgaa ggccccatgt tttctaatgt gtgtaagaaa cggaaatcat
     2101 ttttagtaac catagaacgt ccatcagttc caagtcctgc aacttctagt gtagcccata
     2161 ctgggtcacc agagaataat tggttgtaag aaggaatacg ggcaaattta acc
//
LOCUS       DAOMTA010000539         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2357_length_2212_cov_2.185906, whole genome shotgun sequence.
ACCESSION   DAOMTA010000539 DAOMTA010000000
VERSION     DAOMTA010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_2212_cov_2.185906"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PLK68_12620"
     CDS             <1..1051
                     /locus_tag="PLK68_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HRM91286.1"
                     /translation="KRHGLNQKEKDELLGIDIPQEIITDYEQRRLFNTKINLKVIDFL
                     EAGYLDFLVIPQDDSSPYGYTAISQKIVINALKEKRLDQKVMIYPGADEVGLSLLARA
                     YHEYYHLEPKVYPFYASVLGPMIIPKYEDRPMYESLKSHVRVCKAKLVNNPQDADVVL
                     AINSPGKIMQEAFIDKNDLDVTYTSYRYLLAFAEQIQDYINSGYHVALCDSAFSNGGD
                     LQLIEYLDELNILDQLVSYAGWNTNCNSLGTTLSQAFIGQENVINNLCYRLIEDVFYQ
                     AIVRKEVVENDLPHKGLSYYDFKDQQTDVENIIKKKLQTNFGHLHLSQRYPFNITKIY
                     MPWKRMFEIGMEIKL"
     gene            1108..1929
                     /locus_tag="PLK68_12625"
     CDS             1108..1929
                     /locus_tag="PLK68_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91287.1"
                     /translation="MLSYNRSVIPIIESAYEELTNVEKNIANYFIDQVEDEDLSSKAV
                     SQRLYVSEASLSRFAKKLGFSGYRQFLFAYQDSHQSSRHLDLLTKQVLNSYQKVLEKT
                     YSLIDNDQMVRIAKMLDEYNRVYIYGIGSSSVVAREFKLRFMRLGLDVDYLAESHSIR
                     MNITRVNQESLVIGISVSGKTEEVIEGLREAKEKGAKTIMLSAARVYEYRSYYDELIL
                     IGGLKNLAISDKISPQIPALIVVDILYSHYLNYNNDNKKEKLQMTLEHINYELEE"
     gene            complement(2010..>2212)
                     /locus_tag="PLK68_12630"
     CDS             complement(2010..>2212)
                     /locus_tag="PLK68_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM91288.1"
                     /translation="PAMIVESGLGYVFTFDKLINTTGNCNLCFRPLEPNFETGFYLVW
                     KKYQIFSRSAKMFLEELQKVLF"
BASE COUNT          788 a          283 c          397 g          744 t
ORIGIN      
        1 aaaaagacat gggttaaatc aaaaagaaaa agatgaactt ctcggtattg atattcctca
       61 ggaaattata actgattatg agcagcgtcg tttgtttaat actaagatta atttaaaggt
      121 tattgatttt cttgaagctg gctatcttga ttttctggtt attccacaag atgactcgtc
      181 accttatgga tatactgcaa tatcccaaaa aatcgttatt aatgctttaa aggaaaaaag
      241 attagatcag aaagtaatga tctatcctgg agctgacgaa gtaggtctgt cattattagc
      301 gcgggcttat catgaatact atcatttaga accaaaagtt tatccttttt acgcttcggt
      361 attaggacca atgattattc ctaaatatga agatcgacca atgtatgaaa gtctaaaatc
      421 acatgtacgc gtatgtaaag ctaagctggt caacaatcct caagatgctg atgtggtttt
      481 agcgataaat tcgccaggta agatcatgca agaagctttt attgataaaa atgatttaga
      541 tgtaacttat acaagttatc gttatttatt agcttttgct gaacaaattc aagattatat
      601 aaatagtggt tatcatgtgg cgttatgcga cagtgctttt agtaatggtg gtgatctaca
      661 attaattgaa tatttagatg agctaaatat tttagatcaa ttagtaagct atgcaggatg
      721 gaatacaaat tgtaattcct taggaacaac tttatctcaa gcttttattg gtcaggaaaa
      781 cgtaattaat aatttatgtt atcgtcttat cgaagatgtt ttttatcaag caatagttag
      841 aaaagaagtt gtcgaaaatg atttaccaca taaaggatta tcttattatg attttaaaga
      901 tcaacaaacg gatgtagaga atattattaa aaagaagtta cagactaatt ttggacatct
      961 tcatcttagt cagcgctatc cttttaatat aacaaaaata tatatgccgt ggaaacggat
     1021 gtttgaaatt ggaatggaaa ttaagttata ggaaacgtaa gtttcctttt tatgctataa
     1081 tcatttgtat agtaaaggag gggttaggtg ttatcttata atagaagtgt gattccaatt
     1141 attgaatcag cttatgaaga actaacgaat gtggaaaaaa atattgctaa ttatttcatt
     1201 gatcaagttg aagatgaaga tttatcatca aaagcggttt ctcaaagatt atatgtatct
     1261 gaagcatcct tatcacggtt tgctaaaaag ttagggttta gtggatatcg acaatttcta
     1321 tttgcttatc aagattctca ccaatcaagc cggcatttag atttattaac taaacaagtt
     1381 ttaaactctt atcaaaaagt tttagagaaa acttattcat tgattgataa tgaccaaatg
     1441 gttagaatag ctaagatgct ggatgaatat aatcgagttt atatttatgg aattggctca
     1501 tcgtcagttg ttgcaagaga atttaaatta aggtttatga gattaggatt agatgttgat
     1561 tatttagcag aatcacattc tattcggatg aatattaccc gagtaaatca agaatcatta
     1621 gtgattggaa tcagtgtaag tggaaaaacc gaagaagtta tagaaggatt acgagaagca
     1681 aaagaaaaag gagctaaaac aattatgtta tctgctgctc gggtatatga atatcgttct
     1741 tattatgatg aactgatctt gattgggggg ttaaaaaatt tagctatttc tgataaaatc
     1801 tcaccccaaa ttcccgcctt gatcgtggtt gatatcttat attcacatta tctaaattat
     1861 aacaatgata ataaaaaaga aaagctgcaa atgacattag aacatattaa ttatgaatta
     1921 gaagaataat ctagtatatg gatattggta agctcatatt aaaaagctaa cagagtgctg
     1981 ctttgttagc tttctttgtg aataattaat taaaatagaa ctttttgcag ttcctctaaa
     2041 aacattttag cagaacggct gaaaatttga tattttttcc agacaagata aaatccagtt
     2101 tcaaaatttg gttctaatgg tcggaagcag aggttacaat taccggttgt attaattaat
     2161 ttatcaaatg taaaaacata gcctaaacca ctttctacga tcattgctgg gg
//
LOCUS       DAOMTA010000540         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2371_length_2203_cov_2.093575, whole genome shotgun sequence.
ACCESSION   DAOMTA010000540 DAOMTA010000000
VERSION     DAOMTA010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_2203_cov_2.093575"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..184
                     /locus_tag="PLK68_12635"
     CDS             44..184
                     /locus_tag="PLK68_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91289.1"
                     /translation="MLKTIDPAKNAISTIKRLIATGDDIIIYRLVLVPSLAILVSMKR
                     KH"
     gene            332..889
                     /locus_tag="PLK68_12640"
     CDS             332..889
                     /locus_tag="PLK68_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91290.1"
                     /translation="MKRDVLNKISRVINYEESERVRLNVYVDQLGDQQEEYLLIGEEL
                     ILRLDNKIRKITFDEIKEINVSMCSRLYNPGIVESNLEKLIIKRWTYGSTLAVGQHLN
                     YYVDLDIILDDEKIMIEAYSLKNIVSIIEILSSKGITINDPLKIKDILTKGLDDTALE
                     KYLDNHFPKLAKEYNLDNPRGIIIP"
     gene            1053..1919
                     /locus_tag="PLK68_12645"
     CDS             1053..1919
                     /locus_tag="PLK68_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91291.1"
                     /translation="MLSKVNFRIKTIYNILRPSEKKVADYILNYCGKLEDLSMTLLAK
                     EVAVSQPTVMRFVKAIGYDSFKQFKLELAKNYDKENNIDILYGFSINADDKIMDLPGK
                     IVATATSMLENALKSLSLVNYQKTVALINQSQHISIYAVENSMGVAHDLMTKLIYLGK
                     SVTCHSDYYLQSIDASNLTRDSLAIGISYSGNSKNTVEVLKIAKDAGAQTIAIVNFEN
                     TMLTRYGDIVLSTSNDQFLYGDAIFSRTAQIALVDMIYMGVIISDYDNYTKKLDHYSR
                     LIKHRGYQKEEL"
     gene            2044..>2203
                     /locus_tag="PLK68_12650"
     CDS             2044..>2203
                     /locus_tag="PLK68_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91292.1"
                     /translation="MLKLERIVKSFDGINILNNLSLEIPKGQIVSILGPSGSGKTTLL
                     NLILGISEV"
BASE COUNT          827 a          254 c          380 g          742 t
ORIGIN      
        1 cacccagatt attttgccat agctttccca atttggttta cttatgctaa aaacgataga
       61 tcctgctaaa aatgctattt caacgatcaa aagattaatt gctacaggtg atgatattat
      121 tatatataga ttggtcttag tacctagtct agcaattcta gtttcgatga aaagaaaaca
      181 ttaattaaaa gaatctatgg agtatatata ttcgatacta aatagcgttt tataaaatta
      241 gtatttattg aattaataaa tatttgaata gaatttcttg actattgttt tagaccatag
      301 tgtttaataa aagtgtaaag gggggataat aatgaaacgc gatgttctga acaaaattag
      361 tcgagttatt aattatgagg aaagtgaacg tgttcgatta aatgtgtatg ttgaccagct
      421 aggtgatcaa caagaggagt atcttttgat tggggaagaa ttaatcttaa gacttgataa
      481 taaaattaga aagattacat tcgatgaaat caaagagatt aacgtatcga tgtgttcacg
      541 cttatataat ccaggaattg tggagtctaa tcttgaaaaa ttaataatta aaaggtggac
      601 ttatggtagt accttagcag tcggacaaca tcttaattat tatgtggatc tagatatcat
      661 tttagatgat gaaaaaataa tgattgaggc ttatagttta aaaaatattg tttctatcat
      721 agagatatta tcaagtaaag gaattacaat taatgatccc ttaaaaataa aagacattct
      781 tactaaaggt ttggatgata cagcattaga aaaatacctt gataatcatt ttcctaagct
      841 cgctaaagaa tataatcttg ataacccgcg aggaataatt attccttagt aatctaaaaa
      901 ccagataaaa atctggtttt ttcttttctt aacaaaaaaa ttactttttc tttagataaa
      961 aaaagctagc atgatagtag ctttttttaa tattgcttat actaaaaata aataaaaaga
     1021 gaaaataaag tagaaatact acaggagaga atatgctaag caaagtgaat tttaggataa
     1081 aaacaatata caatatttta cgtccatcag aaaaaaaggt agctgattat atattaaatt
     1141 attgtggaaa gctagaagat ttatcaatga cgttattggc caaagaagtt gctgtatcgc
     1201 agccaactgt aatgcgtttt gttaaagcga taggctatga cagtttcaaa caatttaaat
     1261 tagagttagc taaaaattat gataaagaaa ataatataga tattttatat ggtttttcaa
     1321 taaacgccga tgataaaatt atggatcttc caggtaagat tgtagctaca gcaacatcga
     1381 tgctggaaaa tgcattaaaa tctctttctt tagtgaatta tcaaaaaacc gtagctttaa
     1441 ttaatcaaag tcagcatatt tcaatatatg ctgttgagaa ttcaatgggt gtagctcatg
     1501 atttaatgac taaattaata tatttaggaa agagtgttac ttgtcatagt gattactatc
     1561 tccaaagtat tgatgcaagt aatctgacaa gggatagttt agcgattgga atatcttatt
     1621 caggaaattc taaaaataca gtcgaagtct tgaagattgc taaagatgct ggagctcaaa
     1681 cgattgctat tgttaatttt gaaaatacca tgttaactcg atatggtgat attgttttat
     1741 caacaagtaa tgatcagttt ttgtatggag atgcaatttt ttcacgaact gctcaaattg
     1801 cattagtcga tatgatctat atgggtgtga ttatcagtga ttatgataat tacactaaaa
     1861 aactagatca ctatagtaga ttaattaaac atcgtggata tcaaaaagag gagctgtgaa
     1921 tgtagttttt ctacatctac cacctctttt tattaacgct ttcttaacca agttttaaag
     1981 aaaaaagaat agatagaaat ttatttagtt agctataatg cagttgttcg aggaggaaaa
     2041 agaatgttaa aactagaaag gatcgttaag tcatttgatg gtataaatat tttaaataat
     2101 cttagtttgg agatacctaa ggggcaaatt gtttcaattt taggaccttc aggaagtggg
     2161 aaaactactt tattaaatct tattttagga attagtgagg ttg
//
LOCUS       DAOMTA010000541         2203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2372_length_2203_cov_1.790037, whole genome shotgun sequence.
ACCESSION   DAOMTA010000541 DAOMTA010000000
VERSION     DAOMTA010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_2203_cov_1.790037"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1210..1431)
                     /locus_tag="PLK68_12655"
     CDS             complement(1210..1431)
                     /locus_tag="PLK68_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91293.1"
                     /translation="MNNRGEIWKISDIYNGENIDDKAVVLVIRNNQVYIPTSKLEENI
                     DIIEENCDDIAANNDKYIKNYYNYKVALF"
     gene            complement(1477..>2203)
                     /gene="rsmH"
                     /locus_tag="PLK68_12660"
     CDS             complement(1477..>2203)
                     /gene="rsmH"
                     /locus_tag="PLK68_12660"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM91294.1"
                     /translation="TKERLLGKGYGEDVLTIKNCNFKDIDIISKEVGLFDFVLADLGV
                     SSMQIDNPERGFSYKKEGPLDLRLNPQKGVPASIKLQKLNRDQLEQLFIENSDEPYAK
                     EIARNIDSYLKSGQLITTTTQLYKIIEKSLSSIPVTERKDIVKKSAARVFQALRIEVN
                     REFDVLIEFMDKLPTILKPGGRVAILTFHSGEDRIVKKAFKEMKNLGIYSDVARNVIR
                     PSKEECHMNSRAKSTKMRWAIKA"
BASE COUNT          724 a          365 c          249 g          865 t
ORIGIN      
        1 aatatgaatg gtatcaaaaa tttgtcgaaa gtctagaaat gaatggaatt gctctagaac
       61 aagagatgta tcgattcatt aatattgatg agaatatgtt tccttatttt ctccacgacg
      121 aatttcaaag taagtttgaa aaataatgac agttgaacag tttaaaaata aagattttgc
      181 caactaatat atcaaagaat attttgacga ttcattaccc tatcaacaat tcatttttct
      241 gttttaacca agcataatat ttcaaaagac gaaaattcat atattattac tttacacttt
      301 tatactccta tttacattct tatctcttta tgtaacagta tcccagaacg tgaagatgag
      361 gtagttaata ttttgggact tcgcattaaa cagtttaatc gtttatatgc taataatggt
      421 aaaatttaat gaatcgcatt agcattttta caagttgctt tttatttgca atttattatc
      481 atattgaaaa tcgaaatctt ccttttgttc taccagcatg attatttttc cgatcacgat
      541 atgttttcta tgtttaattt aactttatgc aaaggatacc agtttttaaa aaaatatatt
      601 tctaaccatc agcttaatcc aatacttttt aaaaggactg ttcaaatata taatcacaga
      661 accgtcttta ctttgagttt ctagatttct ttatcaaatt tctctgtctt ctttgcaaat
      721 tttagctggt atccttttaa ctctttattt atcaaaccac aacgttacat ggaatgacgt
      781 atctataatt aagattctat tttcagtaca gccttttagt ttttatccct actccattta
      841 caatttagct ttttgtttag tgtctatatt ggaataagaa tatagacatt atcatcatcc
      901 tgagcatttt tataaaatca gtaataaaca ctcaacttgt gaaaactaca acttagatac
      961 ttgtcataag catgaaacaa aattatttca taaagcactc aaagataaat atcaaaataa
     1021 ataaaaagat aactacaaaa tctaatatat ttaaagttat cctcttattt atatgatttt
     1081 aaaattaatt taatcgtact ctgttttact taaaattatt aaagagctta ttttatatta
     1141 ataatttcca taagtatttt ggaaatatca tcataatcat tgatccaaat gacatttatc
     1201 cccaaactat taaaataatg ctaccttata attataatag ttttttatgt acttatcgtt
     1261 attagctgca atatcatcac aattctcttc aatgatatct atattctctt ctaactttga
     1321 agtcggaata tatacttgat tatttcttat taccaaaaca accgctttat catcaatgtt
     1381 ttctccatta tatatatcac tgattttcca aatttctccc ctattattca tatattagtt
     1441 tagttatctt tcaatatata gtaattccaa gtatacttaa gctttgattg cccatctcat
     1501 ttttgttgat ttagcacgac tattcatatg acactcttct ttacttggtc taatcacatt
     1561 tctagctaca tcactataaa ttccaagatt tttcatttct ttaaatgcct ttttaacaat
     1621 tcgatcttca ccagaatgaa atgttaatat cgcaactctg cctccaggtt taagaatcgt
     1681 tgggagttta tccataaatt ctatgagaac atcaaattca cgattaactt caatccgtaa
     1741 agcttggaat acccgtgcag ctgatttctt cactatgtct tttcgttctg tcactggaat
     1801 agaagacagg cttttttcaa ttattttata cagttgtgtt gtcgttgtaa ttaattggcc
     1861 tgattttaag tatgaatcaa tatttctagc tatttctttt gcgtatggct catcagagtt
     1921 ttcaataaaa agctgctcta actgatctct atttaacttt tgtagcttaa tagatgctgg
     1981 aactcctttt tgaggattta atcttaaatc taagggaccc tcttttttat aggaaaagcc
     2041 ccgttctgga ttatctattt gcatagatga tacccctaga tcagctaaaa caaaatcaaa
     2101 caaaccaacc tcttttgata tgatatcaat gtctttaaag ttacaatttt ttattgttaa
     2161 aacatcttcg ccatatcctt tacctaataa gcgttcttta gtt
//
LOCUS       DAOMTA010000542         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2390_length_2196_cov_2.314806, whole genome shotgun sequence.
ACCESSION   DAOMTA010000542 DAOMTA010000000
VERSION     DAOMTA010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_2196_cov_2.314806"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>2196)
                     /gene="ileS"
                     /locus_tag="PLK68_12665"
     CDS             complement(115..>2196)
                     /gene="ileS"
                     /locus_tag="PLK68_12665"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRM91295.1"
                     /translation="GKGILDTDTSFVIWTTTPWTIPANLAICLNERYDYALIETTKGK
                     LIVLNELVDSLMEKFEITEYKVIKNFKGSELELITCQHPLYDRESLVILGDHVTAEAG
                     TGCVHTAPGFGADDFFVGQKYGLPAYCNVDEHGCMMKDCGEWLEGQYVDDANKTVTMK
                     LDELGVLLKLEFITHSYPHDWRTKKPIIFRATDQWFCSVDKIREKLLSEIDKVNWLNE
                     WGHIRIYNMIKDRGDWCISRQRSWGVPIPIIYCEDGTPIMEEEVFKHISNLFRKYGSN
                     VWFERDEKDLLPEGYINEHSPNGIFKKEKDIMDVWFDSGSSHTGCMIERGYRYPVDLY
                     FEGSDQYRGWFNSSLIVGTAAHGCAPYKTVLSHGFVLDGKGNKMSKSLGNTVDPIKLV
                     NQYGADIVRLWATSVAYQQDVRISNDIMKQISENYRKIRNTMRFVLGNLNDFKQADLV
                     AVEALADVDKYMLAQLNDLIKGYHKAYDNYDFAEANQLILNYFTNLLSSFYMDFTKDI
                     LYIEKADSLRRRQVQTVLYYHAKAMMKLISPVLVFTAEELHDHFHCDDDKAESIFLEP
                     NVEMINMPNSDQLKAHFDRFLELRKDVMKALEGLRNEKIIKSNMEAKVTISLKDEFKE
                     MASMLADLKQLFIVAKVELVKDSSLEEFDSAYIKVEKFEGHQCPRCWNYFDEDEMEGE
                     LCPRCHAVING"
BASE COUNT          732 a          423 c          272 g          769 t
ORIGIN      
        1 caataaaaaa agtaatctaa atcgaacagt catccttggc tatccaagat cagagatgca
       61 gtccaattta tatgattact tttagctata cttatttaaa cttagagatt ttatctatcc
      121 attgattaca gcatgacaac gagggcacaa ttcgccttcc atttcatctt catcaaaata
      181 gttccaacat cgtggacatt gatgaccttc gaacttttca actttgatat aagcgctatc
      241 aaattcttct aacgaagaat ctttcactaa ctcaacttta gcaacaataa ataattgttt
      301 taagtcagca agcattgaag ccatttcctt aaattcatct tttaatgaaa tcgtcacttt
      361 tgcttccata tttgatttta taattttttc atttctcaat ccttctaaag ctttcatgac
      421 atctttacga agctctagga aacgatcaaa atgagctttt aattgatcac tgttaggcat
      481 attgatcatt tcaacattag gttctaagaa aatagattca gctttatcat catcacaatg
      541 gaaatgatca tgtaattctt cagcagtaaa tactagaact ggtgaaatca atttcatcat
      601 tgctttagca tgataatata aaactgtttg gacttgtctt ctacgaagac tatcggcttt
      661 ttcaatataa agaatatctt tagtaaagtc catatagaat gagcttaata aattagtaaa
      721 ataatttaaa attaattgat ttgcttcagc aaaatcataa ttatcataag ctttatgata
      781 tcctttaatc aagtcattta attgagctag catatattta tcaacatcag ccaaagcttc
      841 aactgcaact agatcagctt gtttaaaatc atttaagttt cctaatacga aacgcattgt
      901 atttctaatt ttacgataat tttcagaaat ttgtttcata atatcattac tgattcttac
      961 atcttgctgg taagctactg aagttgccca taatcttaca atatcagctc catattggtt
     1021 aactaattta attggatcaa cagtattacc taatgattta gacattttat tgcctttgcc
     1081 atctaaaaca aatccatgtg ataaaactgt tttgtatggt gcacaaccat gagctgctgt
     1141 tccaacaatc agtgaagagt taaaccagcc acggtattga tcactacctt caaaatataa
     1201 atcaactgga taacgatatc cccgttcaat catacatcca gtatggctgg aacctgagtc
     1261 aaaccataca tccataatat ctttttcttt tttaaagata ccatttggtg aatgttcatt
     1321 tatatatcct tctggtaata aatctttttc atcacgttca aaccaaacat ttgaaccata
     1381 ttttctaaat aaattagaaa tatgtttaaa aacttcttct tccataatcg gtgttccatc
     1441 ttcacaataa atgattggaa tcggtactcc ccaagatctt tgacgagaaa tacaccagtc
     1501 accacgatct ttaatcatat tataaatacg aatatgtccc cactcattta accagttaac
     1561 tttatcaatc tcacttaata atttctctct gattttatca actgaacaga accattgatc
     1621 agtcgctcta aaaataattg gttttttagt acgccaatca tgtggataac tatgtgtaat
     1681 aaattcaagc tttaataaaa ctcctaattc atctagtttc attgtaactg ttttatttgc
     1741 atcatctaca tattgacctt ctagccattc accacagtct ttcatcatac atccatgttc
     1801 atcaacgtta caatatgctg gtaaaccata tttttgtccg acgaaaaagt catctgctcc
     1861 gaatcctggt gctgtatgaa cgcaaccagt ccccgcctca gcagtaacat gatcacctaa
     1921 gatcactaat gattcacgat catataaagg atgttgacaa gtgattaatt ctaactcact
     1981 acctttaaag tttttgatta ctttatactc agtaatttca aatttttcca ttaatgaatc
     2041 aactaattca tttagaacga ttaattttcc tttagttgtt tcaatcagtg cataatcgta
     2101 acgctcattt aaacaaattg ccaggtttgc aggaatagtc caaggtgtag ttgtccaaat
     2161 aacaaagcta gtatcagtat ctaagattcc tttacc
//
LOCUS       DAOMTA010000543         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2399_length_2191_cov_2.308052, whole genome shotgun sequence.
ACCESSION   DAOMTA010000543 DAOMTA010000000
VERSION     DAOMTA010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2399_length_2191_cov_2.308052"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /gene="carB"
                     /locus_tag="PLK68_12670"
     CDS             <1..1733
                     /gene="carB"
                     /locus_tag="PLK68_12670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM91296.1"
                     /translation="FPNQVIAKLTGKTSDEVKNMCQENKINAVYKVVDTCAAEFDATT
                     PYYYSVYGGVNEVNPADDKKKIMVLGSGPIRIGQGIEFDYCSVHCVWALKEAGYDTII
                     VNNNPETVSTDFDIADRLYFEPLTPEDVENIVNIEHPDGAIVQFGGQTAIKLTKALME
                     MGVKILGTSADDVDAAEDRERFDEILEKCEIDRPRGSTVFTIEEALEVANRLKYPVLV
                     RPSYVLGGAGMEICLNDGDVKKFMKIINRQYQEHPILIDKYLAGKEVEVDAICDENGI
                     LIPGIMEHVERAGVHSGDSISVYPTQKIKQHIKDTIVEYTGRLAKALNVIGLINIQFI
                     VYEDQVYVIEVNPRSSRTIPYISKVTDIPVVDVATHVIMGKTIKEQGYEYGLAPEKET
                     IAIKMPVFSFEKIKGAEISLGPEMKSTGEVLGISTDYDEAIYKAFIGSGINLPKKKNI
                     ICTIKDSAKDEFLPIAKQYYMFGYDLYATEGTYNFLKENDVPVSLVNRISAKSNTLFD
                     LMLTDTVDLIIDIPTRNDNLKDGFLIRRFAVEAGIPIFTSLDTAQALITSLEKRHKGD
                     TSLVDITKLK"
BASE COUNT          794 a          252 c          416 g          729 t
ORIGIN      
        1 aattcccaaa tcaagtaatt gctaagttaa caggtaaaac tagtgatgaa gtaaaaaata
       61 tgtgccaaga aaataaaatt aacgcagtgt ataaagtggt tgatacatgt gcggctgaat
      121 ttgatgcaac aactccatat tattattcag tctatggtgg agtaaatgaa gtaaatccag
      181 ctgatgataa gaaaaaaata atggttttag gttcaggacc aatcagaatt ggacaaggaa
      241 ttgaatttga ttattgctca gttcattgcg tttgggcttt aaaagaagct ggttatgata
      301 caattattgt aaataataat cctgaaacag taagtactga ctttgatatt gcagatcgct
      361 tatattttga accattaaca ccagaggatg ttgagaatat tgttaatatc gaacatcctg
      421 atggagcaat tgttcaattt ggtggtcaaa ctgcaatcaa actaacaaag gcattaatgg
      481 agatgggggt taaaatttta ggaacatcag ctgacgatgt tgatgcagct gaagaccgtg
      541 aaagatttga tgagatttta gaaaaatgtg aaattgatcg tcctcgtggt tcaacagtct
      601 ttacaattga ggaggcttta gaagttgcta atcgattaaa atatccagtt ttagttcgtc
      661 cttcatatgt tttaggagga gcaggaatgg agatctgttt aaatgatggt gatgtaaaga
      721 aatttatgaa aatcattaat cgtcaatatc aagaacaccc aattttaatt gataaatatt
      781 tagctggtaa agaagtagaa gttgatgcta tttgtgatga aaatggaata ctgattccgg
      841 gaattatgga acatgttgaa cgtgcgggag tccattcagg tgatagtata tcagtttatc
      901 caactcaaaa gatcaaacag catattaaag atacgattgt tgaatacaca ggtcgtttag
      961 ctaaagcttt aaatgtcatc ggtttaatta atattcaatt tattgtatat gaagaccaag
     1021 tatatgtaat tgaagtaaat cctcgttcaa gtagaacaat tccatatatt tctaaagtaa
     1081 cagatattcc ggtggtagat gtggcaactc atgttattat gggaaaaaca attaaagaac
     1141 agggatatga atatggctta gcaccagaaa aagaaacaat tgcgattaaa atgccggttt
     1201 tctcttttga aaaaattaag ggagctgaga ttagtcttgg tccagaaatg aaatcaacag
     1261 gagaagtgct tggaatttca actgattatg atgaggcaat ttataaagca tttattggtt
     1321 caggaatcaa tttaccaaag aaaaagaata ttatttgtac gattaaagat tctgcaaaag
     1381 atgaattttt accaattgca aaacaatatt atatgtttgg ttatgattta tatgcaaccg
     1441 aaggaactta taatttctta aaagaaaatg atgtaccagt tagtttagtg aatcgcatta
     1501 gtgctaaaag taacacattg tttgatttaa tgttaacaga tactgttgat ttaatcattg
     1561 atattcccac tcgtaatgat aacttaaaag atggtttctt aattagaaga tttgctgtag
     1621 aagcaggaat tccaatcttt acttcattag atactgctca agcattgatt actagtttag
     1681 aaaaacgtca taaaggggat acttctttag tagatattac taaactaaaa tagtttaaaa
     1741 cagacttagt gagattgcat cattaagtct gttttttgta aaatataaac tttgataagt
     1801 agtttcttga taaaattatg gtcaataaca ttattatttt gagttgttaa tgaagataat
     1861 taactttatg tttttttata ttgaagtata attattgaaa gaggtagata atatgtatac
     1921 atatgaaatg ctatatcggt tgtgtagcta agaatctagt gatagtgtga ttgcacacta
     1981 tttattaatg catttaaatt cattggaata gttgatgatt aagaaagtta ttgatgatgt
     2041 agggatctct aaggcttctt tacatcgttt ttttagtaag ggtggctatg agagttttaa
     2101 agacttaatt actatattag atgaagaagt aaaacagaaa aaaatgttta atattgatta
     2161 tcaacagtat cgttccaata attatacttc a
//
LOCUS       DAOMTA010000544         2191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2400_length_2191_cov_1.830993, whole genome shotgun sequence.
ACCESSION   DAOMTA010000544 DAOMTA010000000
VERSION     DAOMTA010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2400_length_2191_cov_1.830993"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLK68_12675"
     CDS             complement(<1..1055)
                     /locus_tag="PLK68_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91297.1"
                     /translation="MEHKQSLLTLTKRLLKIAATLKKFFVISTIVSIIGNIAQMGLMG
                     FGAAFILSVAGKLKYANSVTYCILMIMSGILIVTCRYLEGYFSHAGSYELLAKMRVDM
                     FGTLRKLAPGSLIGRNNGDIMAIAIADIESIEFFFAHTIGPLFTVILLPLVTLIIAGS
                     IDMLFVYALLPIYLIISVIIPILAIKLGRNIGIGYRQKLGELKIFLLDSVYGLSEIQI
                     FDYGKRRNEELEAVNRNINRSIHQLAYHRQLVVSTPTFFIYLARIAVIAVASYLALKG
                     NIDTTGIIILSFLVSASFSSTQSLTTVVSSLLETYAAAQRYFDLEDMVPVVNEIVEPK
                     ELKNIDKIEFINVSFSYP"
     gene            complement(1059..>2191)
                     /locus_tag="PLK68_12680"
     CDS             complement(1059..>2191)
                     /locus_tag="PLK68_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91298.1"
                     /translation="DNLRRKADTFNQSTMSIMKMNFSSFLVSDGLIYLGVILAVVIGC
                     SQLAKGTIDLAQAVLLLMLSYSFFSSIRQLMSATHDALSGIAATIKVDEILNLDASRI
                     HKSEVETAHEIAVKYSQDTLAYLQDYSGIIVKDIVFNYHQRRQVLKQLSIEIPKGKTT
                     AIVGKSGCGKSTLASMLMRFIDPDQGYLFLDGQSYFSLTPEEVRKNIVMVPQRVSIFS
                     GSLEENLKLAAPNASTSELLNVLKQVHLYQWLQSLPEGLSTDLGDSGAKLSGGQRQKI
                     GIARALLSGAPYLIFDEATSSVDRDSEDEIWQCINELGLTRTLIIISHRLSTIQRADN
                     IIVLNQGRLVEQGNHQALLKYHGEYYQLVKEQEALEAAGKVG"
BASE COUNT          763 a          433 c          290 g          705 t
ORIGIN      
        1 ggatagctaa aacttacatt aataaattca attttgtcga tatttttcaa ttctttaggc
       61 tcaacaattt catttacgac gggaaccata tcttccaaat caaaatagcg ttgtgccgcc
      121 gcataagttt caagtaaact tgaaactaca gtcgttaatg attgcgtcga tgaaaatgaa
      181 gccgaaacta aaaatgataa gataataatt ccggttgtat caatatttcc ttttaaagca
      241 agatatgaag ctactgcaat tacagcaatt cgagcaagat aaatgaaaaa cgttggcgtg
      301 gaaacaacta attggcggtg ataagcaagt tgatgaattg aacgatttat attgcgatta
      361 acagcttcta attcttcatt acgacgtttt ccataatcaa aaatttgaat ttctgataat
      421 ccatatacac tatctaataa aaatattttt aattctccta acttttgacg ataaccaatt
      481 cctatattac gacccagttt aatggctaaa ataggaataa taacactaat aattaaatag
      541 attggtaaca gtgcatagac aaagagcata tcaatacttc ctgcaataat caaagtcact
      601 aacggaagta aaataacggt aaataatggc ccaatcgtat gtgcaaagaa aaattcaata
      661 ctttcgatat cagcaattgc aattgccatg atatcgccat tattccgtcc gatcaaactc
      721 cctggtgcca acttgcgtaa agttccaaac atgtcaactc gcattttagc taataattca
      781 tacgaaccag catgtgaaaa atatccttca agataacgac aagtaacaat taaaattcca
      841 ctcataatca ttaaaataca atatgtgacg ctattggcat attttaactt tccggcaaca
      901 ctcaaaataa aagcagctcc aaatcccatt agccccattt gagcaatatt tccaattata
      961 ctgacaattg tagaaataac aaagaatttt tttaatgtgg cagcaatttt caataatcgt
     1021 ttggttaagg ttaataatga ttgtttatgt tccataatct atcctacctt tcccgctgct
     1081 tctaaagctt cttgttcttt taccaactga taatattcac catgatattt taataatgct
     1141 tgatgattcc cttgttcaac taatcttcct tgatttaaca caatgatatt atcagcacgt
     1201 tgaattgtcg ataagcggtg agaaataata attaatgttc tagttaaacc aagctcatta
     1261 atacattgcc aaatctcatc ttcactatca cgatcaacac ttgaagttgc ttcatcaaaa
     1321 attaaatatg gagcaccact taatagagct ctggcgattc caatcttttg acgctgcccg
     1381 ccagataatt tagcaccact atcacccaaa tctgtcgata agccttctgg caaactttgc
     1441 aaccattgat ataaatgcac ttgttttaaa acgtttaata attcacttgt tgaagcattt
     1501 ggtgcagcca gtttcaaatt ttcttctaat gaaccactaa aaatactaac ccgttgtggt
     1561 accataacaa tgttttttct aacttcttca ggcgttaaac taaaataact ttgaccatca
     1621 agaaataaat agccttgatc tgggtcaata aaacgcatta gcatacttgc caacgtactt
     1681 tttccacaac cactcttacc tacaattgca gttgttttac cttttggtat ttcaatactt
     1741 aactgcttta aaacttggcg cctttgatga taattaaaaa caatatcttt tactatgatc
     1801 cccgaataat cctgcaaata agctaaagta tcctgactgt atttaaccgc tatttcgtga
     1861 gctgtttcaa cctcactctt atgaattctt gaagcatcta aatttaatat ttcatctact
     1921 tttattgttg ccgcaatccc tgataaagca tcatgagtcg cagacatcaa ttgtctaatt
     1981 gaactaaaaa aactatatga caacattaac aataaaactg cctgtgctaa atcaatcgtt
     2041 cctttagcca attgtgaaca gccaattact actgccagaa taacacctaa ataaattaat
     2101 ccatctgaaa ccaaaaatga tgaaaagttc attttcataa tcgacattgt actttgatta
     2161 aatgtatccg cttttcgcct caaattatca t
//
LOCUS       DAOMTA010000545         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2401_length_2190_cov_2.429977, whole genome shotgun sequence.
ACCESSION   DAOMTA010000545 DAOMTA010000000
VERSION     DAOMTA010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_2190_cov_2.429977"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLK68_12685"
     CDS             <1..627
                     /locus_tag="PLK68_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRM91299.1"
                     /translation="VDSIITVSNDRLLQLIGGRPMQEAFREADNVLRQGVQTITDLIA
                     IPAFINLDFADVSAVMKNRGNALIGIGMSSGDDKAKEAAKRAISSPLLEVSVAGAKDA
                     IINVTGGPNISLFDANIALETISQEVGDDINTYLGIAINENLDDDIIVTVIATGFEEE
                     NDDDFEPRPNVLKTRSVEDVVPLSARFEDEEDDDDGDFPPAFIKNRRV"
     gene            677..1453
                     /gene="proC"
                     /locus_tag="PLK68_12690"
     CDS             677..1453
                     /gene="proC"
                     /locus_tag="PLK68_12690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRM91300.1"
                     /translation="MKKIGFIGMGNMAGAIAGGIIKSGFVEGENVYAFDIDNDKLTKM
                     HTDFSINVCTSEKELVAMADIVIIAVKPNVVEDVVAKITDELDNKAIISIVAGYDNEM
                     YNELLLDSTRHLTIMPNTPALVMNGMTLFEQENTLTADELNYAVEMFSSIGEVVILPS
                     YQMKAGGSISGCGPAFVYMFIEAMADGGVRLGLPRDVAYRLASQTLIGAGMMQKETQL
                     HPGILKDQVCSPGGITIKGVETLEENGFRNAVLKAIKESN"
     gene            1536..>2190
                     /locus_tag="PLK68_12695"
     CDS             1536..>2190
                     /locus_tag="PLK68_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91301.1"
                     /translation="MEVYIELTYLTNYLIILVALEMMAILISKEMSYLMVIKHSFYLS
                     GVILLLYLDSYSWLIILIWAVVFLCLYQRQIFLYYPIFIFVYFSLLLFASSIIPEAFI
                     YNGILISPVNVSSIGLFIVSLLVVLMQIMFIVYLKRKIRIDDYLYVMKLDYQQHSYTI
                     KGFLDSGNEVYYEGFPLILINQKIIDEYEVIDVLELNDLREDIIEIIKVDQVIINNQR
                     "
BASE COUNT          722 a          252 c          442 g          774 t
ORIGIN      
        1 gtcgattcaa ttattaccgt ttctaacgat cgtttattac aattgatcgg tggacgtcca
       61 atgcaagaag cattcagaga agctgataat gttttacgtc aaggggttca aacaattact
      121 gacttaattg ctattcctgc ttttattaat cttgactttg ctgatgtttc tgcggttatg
      181 aaaaatcgcg gtaatgcttt aattggaatt gggatgtctt ctggtgatga taaagcgaaa
      241 gaggctgcaa aacgtgctat ttcttcgcca ttattagaag tgtctgtagc gggtgctaaa
      301 gatgcaatta ttaatgtaac tggtggacca aatatttcat tatttgatgc aaatattgct
      361 cttgagacga tttctcaaga agttggtgat gatattaata catatttagg aattgcaatc
      421 aatgaaaatt tagatgatga tattatcgtc actgttattg caacaggttt tgaagaggaa
      481 aacgatgatg attttgaacc acgtccaaac gttttaaaaa cacgcagtgt tgaagatgtt
      541 gtacctttat cagcgcgttt tgaagatgaa gaagacgatg atgacggtga ttttccacca
      601 gcatttataa aaaatagaag agtttaatta ataagctaac taaggttagc ttattttgca
      661 aagggggata atttagatga aaaaaatagg atttattggt atgggaaaca tggccggtgc
      721 tattgctggc gggattatta aaagtggttt tgttgaaggt gaaaatgttt atgcattcga
      781 tatagataat gataaactaa caaagatgca tactgatttt tcaataaatg tttgtactag
      841 tgaaaaagaa ttagttgcta tggctgacat agtgataatt gcagttaaac caaatgttgt
      901 tgaagatgta gtcgctaaaa ttacagacga attagataat aaggcaatta tttcaattgt
      961 tgctggttac gataatgaaa tgtataatga attgttattg gattcaacac gacatttgac
     1021 gatcatgccc aatacaccag ctttggtaat gaatggaatg actttatttg aacaagaaaa
     1081 tacgctaact gcagatgaac taaattatgc tgttgagatg ttttcaagca ttggagaagt
     1141 agtaatttta ccaagttatc aaatgaaagc cggaggaagt attagtggtt gtggaccagc
     1201 gtttgtttat atgtttattg aagcaatggc agatggtggg gtacgcttag gcttaccaag
     1261 agatgttgct tatagactag ctagtcagac tttaatcggt gctggaatga tgcaaaaaga
     1321 aacacagtta catccaggta ttttaaaaga tcaagtttgt tcgcctgggg ggatcactat
     1381 caaaggggtc gaaactttag aagaaaatgg ctttagaaat gctgttttaa aagcaattaa
     1441 agaatcaaat taaaatatta ggatcgcaag atcctttttt cttttcttga catttttttg
     1501 cttagataag cgctatgata gttaggggtg aaaagatgga ggtatatatt gaactgacgt
     1561 atctaacaaa ctatttaatt attttggttg ctttggaaat gatggcgatt ttaattagta
     1621 aggaaatgtc gtatctaatg gttattaaac atagctttta cttaagtgga gtaattttac
     1681 tattatactt agatagttat agttggttaa ttatattgat ctgggcagtt gtttttttat
     1741 gtctatatca acgccaaata tttctgtatt atccaatttt tatttttgtt tatttttcat
     1801 tattattatt cgctagttca ataattccag aagcatttat ttataatgga atcttaattt
     1861 caccagtaaa tgttagtagc attggtttat ttattgttag cttgctcgtt gtattgatgc
     1921 agattatgtt tattgtttat ttaaaaagaa aaataagaat tgatgactat ttatatgtaa
     1981 tgaaattgga ttaccagcaa catagttata caattaaagg atttcttgat agtggtaatg
     2041 aagtttacta tgagggtttt cctttaatct taattaatca aaaaattatt gatgagtatg
     2101 aagtaatcga tgttttagaa ttaaatgatt tacgtgaaga tattattgaa ataatcaaag
     2161 ttgatcaggt tataattaat aatcagagat
//
LOCUS       DAOMTA010000546         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2402_length_2190_cov_2.303513, whole genome shotgun sequence.
ACCESSION   DAOMTA010000546 DAOMTA010000000
VERSION     DAOMTA010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2402_length_2190_cov_2.303513"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(90..301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(386..1996)
                     /locus_tag="PLK68_12700"
     CDS             complement(386..1996)
                     /locus_tag="PLK68_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91302.1"
                     /translation="MLKSLIKIRFQGIFMRSMKGSKNKNAGIGKLALMVLVFAYIAIV
                     FGSMFGYMFNMVLEPFTAIGYEWLYFGIMAVLIVLLCFIGSVFTTQQEIYGAKDNEIL
                     LSMPIKTSDILLSRIFVVLIINYLYEALIAIPGGIVYFTQVPFDLLKLLFFLIVIITL
                     PLLALALSCAFGWLMAMLMKRIRNKTIITMVLSLGFLGLYFYLINKLPEYLVALVANG
                     KSIGEAIQNTLFPIYHLALAISDVNVVSLLLYLVCSVGPFVIVMYLLSKNFISIATSK
                     APSKKVKLKDSDIKFSSQKGALFKRELKHFTSNPMVMMNAALGIAFTVVLAGAVLIKG
                     PELLDMLGVIPPEYQALVNEFIVPLLCLAVIGTNSMNIISAALISLEGNRLWIIKSLP
                     IKTKDILESKLMLHLALCVPPGLLFSIVGSVVFSLSLVDSLLVIIVPIVFTVFEALLG
                     LLINLWKPKFDWINETVVVKQSAAVMITMFGTMALVVFIGGIYIGYLNEFIGAVTYVY
                     IMLALFLIIDIAFYYLLNTWGIKRFEEL"
     gene            complement(1989..>2190)
                     /locus_tag="PLK68_12705"
     CDS             complement(1989..>2190)
                     /locus_tag="PLK68_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91303.1"
                     /translation="KGLMRDLCDRGGAIFFSTHVLEVAEKLCDKIAIIKAGKLVVSGN
                     TQDVIGDDSLEEVFLELEDDNA"
BASE COUNT          821 a          467 c          251 g          651 t
ORIGIN      
        1 catcccccat atccagttga tcccacaatt cctactctaa tcatttctag tcctccattt
       61 ctttgttaaa ataaaaaatc gcctcttaaa caagagacga tttaaccgcg ttaccactct
      121 atttatcata agttgaccct atgatctctt tcatcgttaa gggtgattac ccgaaagcga
      181 tactactatt tcccactttc ctctcataaa tgcgcttcat cctaactaga ttaccagcct
      241 tccaccaacg ccagctcgct aaaaccatta ttaagactac tcttttagtc accgagtttg
      301 gttattatta tattactaaa aaaaaatttg tcaaatattt atttgaattt ccaacaattt
      361 aaaagcatgt gtcgtaaaca catgcctata attcttcaaa tcttttaatt ccccaagtat
      421 ttaacaaata gtagaaagca atatcgataa tcaagaaaag cgctagcatt atataaacat
      481 aagttacagc accaataaac tcatttaaat aaccaatata aataccacct ataaaaacta
      541 caagcgccat cgttccaaac attgtaatca ttacagcagc actttgtttt acgacaacag
      601 tctcattgat ccaatcaaat ttcggcttcc ataaattaat caataaacct aataaagctt
      661 caaaaaccgt aaaaactata gggacgataa taactaataa actatctact aaactcaatg
      721 aaaaaactac acttccgaca attgaaaata acaatcctgg tggtacacat aaagctaagt
      781 gtagcatcaa tttactttca agaatatctt tagttttaat tggtagtgat ttaatgatcc
      841 atagacgatt cccttccaac gagatcaaag cagcactaat aatattcatt gaattagtcc
      901 caatcactgc caaacataac aatggaacta taaattcatt caccaaagct tgatattctg
      961 gtggaattac tcccaacata tctaaaagct caggaccttt aatcaagaca gcaccagcta
     1021 aaacaacggt aaatgcaatt cccaaagcag cattcatcat gaccatcgga ttagatgtaa
     1081 aatgttttaa ctctcgttta aataacgctc ccttttggct actaaattta atatctgaat
     1141 ccttcaattt aactttcttt gatggtgctt tactagttgc aatactaata aaattcttcg
     1201 aaagaagata cattacaatt acaaatggcc ctacactaca aactaaatat agtaacaaac
     1261 tcacgacatt gacatcactg attgctaaag ctaaatgata gattggaaat aacgtatttt
     1321 gaatcgcttc accaatcgat ttaccattag ctactaatgc aaccaaatat tccggaagtt
     1381 tattaatcaa ataaaaatag agtcctaaaa atcctaacga taataccata gtaataattg
     1441 ttttattccg tattcgtttc attaacattg ccattaacca gccaaatgca caagataaag
     1501 ctaaggctaa taacggcaac gttataatga caataaggaa aaagagtaat tttaaaagat
     1561 caaacggcac ctgagtaaaa taaacaattc caccagggat tgcaatcaat gcctcatata
     1621 aatagttgat gattaatacc acaaaaatac gacttaataa aatatcactt gttttaatcg
     1681 gcattgataa taatatctca ttatctttag caccataaat ttcttgttgg gttgtaaaaa
     1741 cactcccaat aaagcaaagt aaaacaatta atactgccat gattccaaaa tataaccatt
     1801 cataaccaat tgccgtaaat ggttctaaaa ccatattaaa catgtagcca aacattgaac
     1861 caaaaacaat ggcaatataa gcaaacacta ataccatcaa tgctaattta cctattccgg
     1921 catttttatt tttacttcct ttcatcgatc tcataaaaat tccttgaaac cgaatcttaa
     1981 tcaatgactt aagcattgtc atcctccaac tctaggaaca cttcttctaa tgaatcatcc
     2041 ccaatgacat cttgggtgtt cccggatacg acaagtttac ccgctttaat aatagcaatt
     2101 ttatcacaaa gtttctcagc tacttcaaga acatgagttg aaaaaaagat tgctccacca
     2161 cgatcgcaca aatcccgcat caaacccttt
//
LOCUS       DAOMTA010000547         2184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2419_length_2184_cov_2.604509, whole genome shotgun sequence.
ACCESSION   DAOMTA010000547 DAOMTA010000000
VERSION     DAOMTA010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2419_length_2184_cov_2.604509"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLK68_12710"
     CDS             complement(<1..225)
                     /locus_tag="PLK68_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91304.1"
                     /translation="MKENFLWGGALAASQCEGAYNLDGKGLSVSDVMLAGSKTKKRKR
                     TDGIVAGEYYPSHEAINFYHYYKDDLQYFKE"
     gene            complement(286..720)
                     /locus_tag="PLK68_12715"
     CDS             complement(286..720)
                     /locus_tag="PLK68_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HRM91305.1"
                     /translation="MLGINNSGRFINRTLLDEIVMTNNKQRYQYNEDHTKIRANQGHS
                     IKVDIELIEKIPPEYLYHGTAFKYLNKIEQEGIKKMKRLYVHLSKDIETAFKVGSRHG
                     KAIVLVIDTKAMCEDGCKFYYSQNGVWLTEDIDYKYVMEVIK"
     assembly_gap    913..922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<923..1228)
                     /locus_tag="PLK68_12720"
     CDS             complement(<923..1228)
                     /locus_tag="PLK68_12720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91306.1"
                     /translation="MIIRKATIKDLKAITAVEATCFPPAEAASRSNFKKRLKTYPNHF
                     WLLEDEGKLISFINGMVTNETTINDIMFEDASLHDEAGEWQAIFGVNTIPEYRQQGLA
                     "
     gene            1374..2111
                     /locus_tag="PLK68_12725"
     CDS             1374..2111
                     /locus_tag="PLK68_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRM91307.1"
                     /translation="MPRPCRKRKVCRLPRYAYFGPNPSATKTVVLSIDEYETIRLMDL
                     EGLTQQECAKQMDVARTTVQSIYEQARYKLAQSIVRGYSLKIEGGNYSLCDKAHLVNC
                     TSSCTIKNKRKMEDNRMKIAVTYEDGNIFQHFGKSKEFKVYDIENQEIISSQIESTNG
                     QGHSALAEILKNLNIDVLICGGIGGGARNILTSLGIEIIPGVIGSSDEAVIDYLKGEL
                     HYDPNTACNHHDDGHHACHEHDNKCCH"
BASE COUNT          741 a          413 c          297 g          723 t
ORIGIN      
        1 ttctttaaaa tattgtaaat catctttata gtaatgataa aaattaattg cttcatgact
       61 tggataatat tctccagcaa caatcccatc agtccgtttt cgttttttag tcttacttcc
      121 tgcaagcatt acatcactaa cacttaatcc cttaccatcg agattatacg caccttcgca
      181 ctgactcgca gccaaagcac caccccataa aaagttttct ttcatcgttt tccacctcat
      241 acttattatt ataacataga aaataaaaag caatggtatt aaccatcact taattacttc
      301 cattacatat ttataatcaa tgtcctcagt taaccacaca ccattttggg aataataaaa
      361 tttacagcca tcttcacaca ttgcctttgt atcaataact aaaacaattg ctttaccatg
      421 tctacttccg actttaaatg ctgtttcaat atctttcgat aaatgaacat ataaacgttt
      481 cattttttta atcccttctt gctctatctt gttcaaatat ttaaatgccg ttccatgata
      541 aagatattct ggtggaatct tttcaattaa ttcaatatct actttgattg aatgaccttg
      601 gttagctcga atttttgtat gatcttcatt atattgatat cgctgtttat tatttgtcat
      661 gacaatctca tctaataatg ttcgattaat aaaacgacca ctattattaa ttcctaacaa
      721 caactcatca acctttgccc atccatcacc accaatgatc cattgagatt tactaaatca
      781 ttcatgttca aactctaaac gcatatcata ccagatagca ccaccatgca ttgactgaga
      841 aatcccacaa tttttaaatc caaacttctc ataataatgc aataatttat ccttgcaagt
      901 aagaatacat ccnnnnnnnn nnagctaacc cttgttgacg atattcagga attgtattta
      961 ctccaaaaat tgcctgccat tctcctgctt catcatgtaa gctagcatct tcaaacataa
     1021 tatcattgat cgttgtttca tttgttacca ttccgttaat aaaactaatc aattttcctt
     1081 catcttccaa tagccaaaaa tgattcggat aagttttcaa acgtttttta aaattactcc
     1141 gactagctgc ttctgcaggt ggaaaacaag tggcttcaac agctgtaatt gcttttaaat
     1201 ctttaattgt tgctttacgt attatcattg ttaaaacctc ctttactcgt ttagagtata
     1261 tcacttatta taaaattaat aaagaatcat tgaatagtgg ttaattattg ctatttttat
     1321 aatattagct tataatgtta ttgacatatg tcaataacaa aggtggtgaa gctatgccaa
     1381 gaccatgtcg aaaaagaaaa gtatgcagat tacctagata tgcatatttt ggtccaaacc
     1441 cgtcagctac aaaaacagta gtcctatcaa tcgacgaata tgaaacaatt agattaatgg
     1501 atcttgaagg actcacacaa caagaatgtg ctaaacaaat ggatgttgcc cgaacaaccg
     1561 ttcagagtat ttatgaacaa gcacgttata agcttgctca aagcattgtc cggggctatt
     1621 ctttaaaaat tgaaggtggt aactactctc tttgtgacaa agctcactta gtcaattgca
     1681 cttcatcttg tacaattaaa aataaaagaa aaatggagga taacagaatg aaaatagcag
     1741 taacttatga agatggtaat atttttcaac attttggaaa gagtaaagag tttaaagtat
     1801 acgacattga gaatcaagaa attatctcta gtcaaataga aagtactaac ggtcagggtc
     1861 attcagcctt agcagaaatt ctaaaaaatc ttaacattga tgtattgatt tgtggtggaa
     1921 ttggcggagg tgcacgaaat atcctcacat cattaggaat cgaaatcatt cctggggtca
     1981 ttggcagtag tgatgaagca gtaatcgact atctaaaggg tgagttgcat tacgatccta
     2041 acacagcctg taatcaccat gatgatggtc atcacgcttg tcatgaacac gataataaat
     2101 gttgtcatta aaaagatgag actaaaaact atagtctcat ctttttaaaa ttcaacttta
     2161 attaattcta catcattaga aacc
//
LOCUS       DAOMTA010000548         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2424_length_2182_cov_2.068641, whole genome shotgun sequence.
ACCESSION   DAOMTA010000548 DAOMTA010000000
VERSION     DAOMTA010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2424_length_2182_cov_2.068641"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLK68_12730"
     CDS             <1..746
                     /locus_tag="PLK68_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRM91308.1"
                     /translation="MVPCCENQNLYVYLSRFKKTIITIKIERKQIMANVVLIGATGFV
                     GSAILNELVERAHKVTAVVRNTSRLPKNNNITAIEEDVANVDAIAKLSEGKDAIISSY
                     NPGWMNPQITELLYSNYPKILEATKKSGVKRLLIVGGAGTLFVKPGVRLIDTGVIPDE
                     IMGGVRPLADFYLKTLVNENDIDWVFFSPAGTFDKEGKKTGKFRLGSDDLVVDAQGNS
                     HISVQDYAIAMVDELEQVKHHKERFTIGY"
     gene            1494..>2182
                     /locus_tag="PLK68_12735"
     CDS             1494..>2182
                     /locus_tag="PLK68_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM91309.1"
                     /translation="MKKRKIFYLLVSMLLLCGFTSPKQDLKITYLGNCSYLYESARSK
                     VVIDPFGTEYGNYFYLPSNDTKQNIIKGNAPFDKVNLLLITHIHGDHFNAKLTEKFLL
                     NNRKAKMICPQQVYMQMRDSCKSFAHIKSQIISPDLAMNGSKKINANGVPITVIRMQH
                     GTNRSLVGIDYSNYTNYEKTEDFGYVIHFGKKTVFHQGDGCLKINGKALNKIDCPIDI
                     AYLGFFDWDSAS"
BASE COUNT          770 a          354 c          385 g          673 t
ORIGIN      
        1 acatggtgcc ttgttgcgaa aatcaaaatt tgtatgtata tttgtcccga tttaagaaaa
       61 caataataac aataaaaata gaaaggaaac aaattatggc aaatgtagtt ttaataggtg
      121 caaccggttt tgtaggttca gccattttga acgagctggt agagcgtgcc cacaaagtaa
      181 cagctgttgt tcgcaacaca tcaagactgc caaagaataa caatattaca gccattgaag
      241 aggatgtagc taatgtagat gccattgcca agttgtctga gggcaaggat gctatcatat
      301 cttcttataa tccgggatgg atgaatccac aaattacaga actcctatac agcaattatc
      361 ctaagattct tgaggcaacc aagaaaagcg gtgtaaaaag attgcttatt gtaggcggag
      421 ccggtactct atttgtaaaa ccaggggttc gtttgattga taccggtgtt attccggatg
      481 aaatcatggg tggtgttcgt cctctcgcag acttctatct caaaacattg gtgaacgaga
      541 atgatatcga ttgggtattc ttctctcctg ccggcacatt cgataaggaa ggtaagaaaa
      601 cgggaaaatt ccgccttgga agcgacgatc ttgttgttga tgctcaaggc aatagccaca
      661 tatccgttca agattatgca atagcaatgg ttgatgaatt agagcaagtt aagcaccata
      721 aagagcgatt tactattgga tactaaacaa cagtcctatt tatatataat gtctcggttc
      781 aattgaactg gggcattttt atattttata aaaaaataca ttttaatatc aaaacctcac
      841 taaaagctag taactaactg tgtataagat tgttttgtca agtgaggttt agtgaggtta
      901 gtgaggttaa cctcactcta attagtatcc ttttaccttc caaaagcttt gataataaag
      961 aacaaatcaa cttattttgt taagccaatc aacttgtttt gtcagacaaa tcaacttgtt
     1021 ttgtacaaat aatgaattcg tttcatcaac ataaggcgcc actacccttt cgcgatttta
     1081 atactaaaag gcaatgttat tatatatttt gcagtttaca attttagtga agttaacctc
     1141 actaacctca ctaaacatca ctccattaaa ttaactgata ttcaacacat tacactattt
     1201 tagtgaggtt tgatattaaa aaacattttt tcgaaaaaaa tattttaata ttaaaatgga
     1261 taaaccaaag cactgttcaa aatagaaaca gctctcttat cattgtttta aataaaataa
     1321 gatctacagc gctgagatat aatatttaca tacagaaaag acaccggaaa tgttcttttt
     1381 aattattgat attttgtagt gattgagaaa ataattatct ttgtggatta aaccaagaaa
     1441 aacgaccata aaacaataaa cagattagca gaaataataa aataaacaaa atcatgaaaa
     1501 aacggaaaat attctatttg ctagtttcca tgctcctttt gtgtggattt actagcccca
     1561 agcaagactt gaaaattacc tatttgggaa attgtagtta tctttatgaa agcgctagat
     1621 ccaaggtagt cattgaccct tttggaactg agtatggtaa ctatttttat ttgccttcaa
     1681 atgatacaaa gcagaatatt attaaaggaa atgcaccttt tgataaagta aacctacttc
     1741 tgatcacgca cattcatgga gaccacttta acgccaaatt gacagaaaaa tttcttttaa
     1801 ataatcgtaa ggccaaaatg atttgtcccc aacaagttta tatgcaaatg agagattcgt
     1861 gcaaaagttt tgctcatata aagtctcaaa tcattagccc tgatttagca atgaatggat
     1921 cgaaaaaaat aaatgcaaat ggggttccaa ttaccgtgat acgtatgcag catggcacta
     1981 atcgcagtct tgtgggtata gattattcaa actatacaaa ttatgaaaaa acagaagact
     2041 ttggatatgt tatccacttt ggcaagaaaa cggtatttca tcaaggcgat ggttgcctga
     2101 agataaatgg gaaagctctg aataagattg attgtccaat agatattgca tatttaggat
     2161 ttttcgattg ggattcagca tc
//
LOCUS       DAOMTA010000549         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2429_length_2179_cov_2.132298, whole genome shotgun sequence.
ACCESSION   DAOMTA010000549 DAOMTA010000000
VERSION     DAOMTA010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_2179_cov_2.132298"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLK68_12740"
     CDS             complement(<1..568)
                     /locus_tag="PLK68_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRM91310.1"
                     /translation="MKKNVEIKKLGINGEGIGYIDRKIVFVPGALPQEEVIVEIIKQT
                     RTYSEGKLLQVVKPSKDRVTPKCRSYDNCQGCTMLHLSYFKQLNAKKEAVRESIRKYT
                     EYDLSRTIFKDVIAAPSQEGFITAVNLPIVDFKGKISFGIYQRDSKYLTLMTRCFKQH
                     PIINECLLKLEEILTTNKCKTYSDKFRTG"
     gene            complement(601..1887)
                     /locus_tag="PLK68_12745"
     CDS             complement(601..1887)
                     /locus_tag="PLK68_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM91311.1"
                     /translation="MDPTQITNVIIFVILIIFSGIFSCTETAFLSVNKIRMRNLAEEG
                     NKRALTVEKLLSNSDRLFSSILVGNNLVNIGASSLSTSFVISIFGDSATLVAAATGVV
                     TFLILVFGEITPKSFATKNADTIALFLARFVALVCTLFTPVVFFLNIITSFFIKILGG
                     ARDSGPTMTEEDLKTIVTVGHEEGVLEEQEKEMIHNVFEFGETEIKEIMTPRIHVESI
                     PDDCSYQELMEIYQRSQFSRYPVHSESFDEIVGVLNVKDLLFFNIDPDEFVVKDFMRD
                     TFVVYEFNEVADVFASMRKEHATLAIVLDEYGVMSGIVTFEDIVEEIVGEIDDEYDAE
                     EDEMIISLGENEYLIDGSLNLNEVNDRVGTDFDSEDFESIGGLVLGEVSGVPEIDDEV
                     QIENVIFKIVKMHKNRIAQLKVTILEEKKDEEEEHH"
     gene            complement(1966..>2179)
                     /locus_tag="PLK68_12750"
     CDS             complement(1966..>2179)
                     /locus_tag="PLK68_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRM91312.1"
                     /translation="KKVSSQRQKAYTFMDTKTLKVENYIGKSKKEVSNPELKFEFIGE
                     GDKVIDQLPRVGESVEAGSTIVIMLG"
BASE COUNT          721 a          443 c          246 g          769 t
ORIGIN      
        1 atcctgttct aaatttatca ctatatgttt tacatttatt tgtagttaaa atctcttcta
       61 attttaataa acactcatta attattggat gttgtttaaa acatcttgtc attaatgtta
      121 aatatttact atcacgttga tagattccaa aactaatttt ccctttaaaa tcaacaatag
      181 gcaaattaac agcagtaata aagccctctt gactaggcgc cgcaattaca tctttaaaaa
      241 ttgttcttga taaatcatac tctgtatatt ttctaattga ttcgcgtaca gcttcttttt
      301 ttgcattcaa ctgcttgaaa taacttaaat gcaacatcgt acacccttga caattatcat
      361 acgaacgaca cttaggcgta acacgatctt ttgatggttt tactacttgt agtaatttcc
      421 cttcgctata agttctagtc tgtttaataa tttcaacaat aacttcttct tgtggcaacg
      481 ctccaggaac aaaaacgatc tttcgatcaa tatacccgat accttcacca ttaatcccta
      541 attttttaat ttctacattt ttcttcatcc tacaatctcc tttttaaaag aggaagtcat
      601 ctagtgatgc tcttcctctt catctttctt ttcttctaga atcgtcactt ttaattgagc
      661 aattctgttt ttatgcattt ttacaatctt aaaaattacg ttttcaatct gaacttcatc
      721 atcaatttca ggaacaccag atacctctcc aagaactaat ccaccaattg attcaaaatc
      781 ttcagaatcg aaatcagttc ctactctatc attaacttca tttaaattca agcttccatc
      841 tattaaatat tcattttctc ctagagaaat gatcatttcg tcttcttcag catcatattc
      901 atcatcgatt tcgcctacaa tttcttcaac aatatcttca aaagtaacaa ttccactcat
      961 aaccccatat tcatctagta caatcgctaa tgttgcatgt tctttacgca tcgaagcaaa
     1021 cacatccgca acttcattaa attcataaac aacaaaagta tcgcgcataa aatcttttac
     1081 aacaaattca tcaggatcga tattaaagaa aagtaaatcc ttaacattta aaactcccac
     1141 aatttcgtca aatgattcac tatgcactgg gtaacgtgaa aattgactac gttgataaat
     1201 ttccatcaat tcttggtaag aacaatcatc gggaatactc tcaacatgaa ttcttggcgt
     1261 cataatttct ttgatctcag tttcaccaaa ctcaaataca ttatgaatca tttccttttc
     1321 ctgttcttct aataccccct cttcatgacc tactgtaacg atcgtcttta aatcttcttc
     1381 tgtcatggtt ggtccactgt cacgagcacc acccaatatt ttaatgaaaa agcttgtaat
     1441 gatatttaag aaaaatacca ctggggtaaa aagagtacaa actaaagcaa caaatcgtgc
     1501 caaaaataaa gcaattgtat ctgcattctt agttgcaaaa gattttggtg taatttcacc
     1561 aaaaaccaaa atcaagaatg taactactcc agtagctgca gctactagag ttgctgaatc
     1621 tccaaaaatt gaaatcacaa atgatgtaga taacgatgaa gcaccgatat taactaagtt
     1681 atttcctact aagattgaac taaataaacg atccgaatta gataacagct tctctactgt
     1741 tagtgctctt ttatttcctt cttcagctaa gtttcgcatc ctgattttat taacagataa
     1801 aaatgctgtt tcagtacaag aaaaaatacc tgagaaaata attaaaatga caaatattat
     1861 aacgttggtt atctgggtcg ggtccacaat aaatctattc tccttattat taatgttttt
     1921 cattatcata ttataaacat tataaaaagt caaattaagc tttaattaac caagcataat
     1981 aacgattgtt gatccggctt caactgactc accaactcgt ggtaattggt ctattacttt
     2041 atcaccttcg ccaataaatt caaattttaa ttccggatta ctaacttcct ttttactttt
     2101 cccaatatag ttttcaacct ttaacgtttt agtatccata aatgtataag ctttttgccg
     2161 ctgtgaagaa acttttttt
//
LOCUS       DAOMTA010000550         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2432_length_2175_cov_3.694340, whole genome shotgun sequence.
ACCESSION   DAOMTA010000550 DAOMTA010000000
VERSION     DAOMTA010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_2175_cov_3.694340"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1674
                     /locus_tag="PLK68_12755"
     CDS             256..1674
                     /locus_tag="PLK68_12755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM91313.1"
                     /translation="MLHKKLKPFPSDFLWGASTSAYQVEGANLIDGKGPSCQDVKKVP
                     EGTSELDVCADQYHRYKEDIALMAEMGFKTYRFSIAWTRILPNGTGEVNPKGIEYYNN
                     VINECLKYGIEPLVTMFHFDMPAALDERGSWGNPESVDWFVNFAKVMYENYGDRVKYW
                     LTINEQNMLTLVGPVIGTLHLPEGCTNEIKEIYQQNHHMLVAQAKAMALCHEMIPGAK
                     IGPAPNISLVYPASCKPEDVLAAQNYNAIRNWLYLDMAVYGVYNNLVWAYLEEHDACP
                     TFAPGDAEALKNGHPDFIGFNYYNTATCEASDGTETMDPGADQQTARGEAGFYRGFKN
                     PNLPTTELGWEIDPMGFRATIREMYSRYRLPMIVTENGLGAYDKLTEDGKVHDQYRIE
                     YLRKHLEQVQLAITDGCEMMGYCPWSAVDLISTHEGMVKRYGFIYVDREEFDLKTLDR
                     YRKDSFYWYKKVIATNGDDLSD"
BASE COUNT          745 a          347 c          356 g          727 t
ORIGIN      
        1 acaattataa caaattcatc acattagtcc aagataaaaa aatttttctc tagtttataa
       61 aataaagcaa ttattagaat aaaatgcata tttttcattt ttaaaggtta tatttagcca
      121 atatgtatta cccttacact tagtaaatct ttgattttac catccctctt tagtgctttt
      181 ttcatatttt gaaaaacggt tccatttaca ttactttaag gtataataaa gccgtaaaaa
      241 caaggaggaa ataaaatgtt acacaaaaaa ctaaaaccat tcccaagtga ttttttatgg
      301 ggagcttcaa catctgctta tcaagtagaa ggagctaact taatcgatgg aaaaggacca
      361 tcttgtcagg atgtcaaaaa agtacctgaa ggaacttcag aacttgatgt gtgtgctgat
      421 caatatcatc gttataaaga agatattgca ttgatggcag aaatgggatt caagacttat
      481 cgtttctcta tcgcttggac aagaatttta cctaatggaa ctggtgaagt caatccaaaa
      541 ggaattgaat attataataa tgttattaat gaatgtttaa aatacggtat tgaaccattg
      601 gtaacaatgt tccattttga tatgccggct gctttagatg aaagaggaag ctggggtaat
      661 ccagaatcgg ttgactggtt tgtaaacttt gcaaaagtta tgtatgaaaa ttatggtgat
      721 cgtgttaaat attggctaac gatcaacgaa caaaatatgc tgactttagt tggaccggta
      781 attggtacat tacacttgcc ggaaggatgc acaaacgaaa tcaaagaaat ctatcagcaa
      841 aaccatcata tgctggtagc acaagctaaa gcaatggcat tatgccacga aatgattcct
      901 ggagcaaaaa tcggacccgc accaaatatt tcattagttt atcctgcttc ttgtaaacct
      961 gaagatgttc tagcagcaca aaactataat gctataagaa actggttata tttggatatg
     1021 gcggtttacg gtgtatataa taatttagtt tgggcttact tagaagaaca tgatgcctgc
     1081 ccaacatttg cacctggaga tgctgaagca ttgaaaaatg gacatcctga ttttattggt
     1141 ttcaactatt acaatactgc tacttgtgaa gctagtgatg gaactgaaac aatggatcct
     1201 ggagctgatc agcaaactgc tcgtggtgaa gccggattct atcgtggatt taaaaatcct
     1261 aatttaccaa ctactgaatt aggatgggaa attgatccaa tgggattccg ggcaacaatc
     1321 cgtgaaatgt actcacgtta tcgtctgcca atgatcgtta ctgaaaatgg actaggagca
     1381 tatgataaat taacagaaga cggtaaagta catgatcaat atcgaattga atatttaaga
     1441 aaacatcttg aacaggtaca attagcaatc acagacggtt gtgaaatgat gggatactgt
     1501 ccatggtcag cagtagactt gatttcaaca catgaaggta tggtcaaacg ttatggattt
     1561 atttatgtag accgtgaaga atttgattta aaaactttag atcgttatcg taaagattca
     1621 ttctattggt acaaaaaagt tattgccact aatggtgatg atttatcaga ttaatttaat
     1681 cttaaaaagt cttctataga aatctataga aggctttttt gctaataaat atagtattat
     1741 aacaacatgt tttctattta atttaagtaa atttgctaat ctacaatctt ttcaaatacc
     1801 gggtcataac taactaatta attattgttt caccttcttg ttgcaattat tttaacgctt
     1861 caatcacaat ttatctatta ttctgatcaa ctgcctaagc ctgctcaccc accaagattt
     1921 cttcctctga acatttaata tccttatatc cttgtagtgt ttgtttatat aaagccattt
     1981 ttatttttcc ttctcatttc ctttttcata ttctaaagct aattttaaat catattccat
     2041 tattttattt tctattaagg caaaactttg aaaaagcaac taatcatttc cacttttcta
     2101 gcgaatagct taattttttt atcaaataaa ataatattcc cacttgttaa tgaattcaat
     2161 cattccttta aaata
//
LOCUS       DAOMTA010000551         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2439_length_2172_cov_2.043458, whole genome shotgun sequence.
ACCESSION   DAOMTA010000551 DAOMTA010000000
VERSION     DAOMTA010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_2172_cov_2.043458"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLK68_12760"
     CDS             <1..818
                     /locus_tag="PLK68_12760"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRM91314.1"
                     /translation="KCVGNKAASGTAILDEYGEHQIKTGDWIVYTSADSVFQIAANEE
                     IIPLEELYKACEIAREIAMDERWKVGRVIARPYVGKRVGEFKRTANRHDLALAPFGRT
                     VLDNLKDKQFDVIGVGKIPDIFVDQGITKAVKTVSNQDGMEKTVELAKSEFKGLCFVN
                     LVDFDAVYGHRRNPEGYGQAIVDFDKQLEELMKYLNHDDLLMITADHGNDPTYSGTDH
                     TREQVPLIIYSKELLKPRHLHDMESFAVIGATIADNFGIELPTIGQSILELIK"
     gene            827..1093
                     /locus_tag="PLK68_12765"
     CDS             827..1093
                     /locus_tag="PLK68_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91315.1"
                     /translation="MEDNTKRLIVMSILAYAIGTFIFAAGLMTKTAVSIILFYIIASI
                     LIICGILALYNNYKKNHQIKLYLYLIVVGIVFLFLNTTVLINNL"
     gene            complement(1259..>2172)
                     /locus_tag="PLK68_12770"
     CDS             complement(1259..>2172)
                     /locus_tag="PLK68_12770"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM91316.1"
                     /translation="LIENALYVARDKYGLRPVSIGQLPNGAYVFSSETCAFEVVGAKF
                     VRDLEPGEIVRVKDGQIKSKLYTNDVTDKICAMEYIYFSRPDSNLDGINVHTTRKLAG
                     KQLFKEAPVEADLVIGVPDSSISAAIGYAEAANIPYEMGLIKNKYVGRTFIQPTQEMR
                     EQGVRMKLSAVSSIVKGKRVVMIDDSIVRGTTSKRIVRLLKEAGAREVHVRIASPAIK
                     YPCFYGVDMSTMDELISNRLNVNELCNYIEADSLAFITEEGIDKSIHFNKEKHKCSLC
                     LACFNGEYVTNLYDSFEMDKIKKGKEY"
BASE COUNT          747 a          324 c          354 g          747 t
ORIGIN      
        1 gtaaatgtgt tggcaataag gctgcaagtg gtacagcaat tcttgatgag tatggtgaac
       61 atcagataaa aacaggtgat tggatcgtat atacttcagc tgattcagtt tttcaaatag
      121 ctgcaaatga agagattatc ccgttagaag aattgtataa agcatgcgaa attgctcgtg
      181 aaatagccat ggatgagcgt tggaaagttg gtagagttat tgctcgacca tatgtaggaa
      241 aaagggtagg ggaattcaag cgtactgcca atcgtcatga tttggcctta gcaccatttg
      301 gacggactgt tcttgataat ttgaaagata aacagtttga tgttattgga gtcgggaaaa
      361 ttccggatat ttttgttgat caaggaatta ctaaagcagt taagacggta agtaatcagg
      421 atggaatgga aaaaactgtt gaacttgcaa aatcagaatt taaaggattg tgttttgtta
      481 atcttgtaga ttttgatgca gtatacggtc atcggcgtaa tcctgagggc tatgggcaag
      541 caattgtaga ttttgataag caattggagg aattaatgaa atatttaaat catgatgatt
      601 tattaatgat cactgctgat catggtaatg atccaactta tagtggtact gatcatactc
      661 gtgaacaagt tccattaatt atttattcaa aggaattatt gaagccacgt catttacatg
      721 atatggaatc ttttgctgta attggtgcta caattgcaga taattttggt atcgaattac
      781 caactattgg ccaatcaatt ttagaattaa ttaaatagga gaatttatgg aagataatac
      841 aaaacgattg atcgtgatgt caattttagc ttatgcaatt ggaacgttta tctttgctgc
      901 aggattaatg accaagacag ccgttagcat aatattattt tatataattg caagcatatt
      961 aataatttgc ggtattttag cattatataa taattataaa aaaaatcatc aaattaaatt
     1021 atacttatac ttaatagtgg taggaattgt tttcttgttt ttaaatacaa cggtattaat
     1081 taataactta tagtttataa aagcattcta agattattgg aatgctttta attaggattt
     1141 atcgatcaat ttatttctgt ttgtctatta aaaattaatt ataaaattta cataatatac
     1201 attatgcgaa gttatatatg ttatatctac tttaataatt ataagaggta aggagttttt
     1261 aatattcctt acctttcttg attttatcca tttcaaatga atcatataaa tttgttacat
     1321 attcaccatt gaaacaagct aaacataaac tacatttatg tttttcttta ttaaaatgga
     1381 tagatttatc aattccttct tcagtaataa aggcaagtga atcagcttca atataattgc
     1441 ataattcatt aacattcaaa cgattagaaa ttaattcgtc catggttgac atatcaacac
     1501 cataaaaaca aggatatttg attgctggtg aagcaattct tacatgcact tctctagcac
     1561 cagcttcttt taacaatcgt acaattcttt tagaggtagt tccacgtaca attgaatcat
     1621 caatcataac aacacgtttt cctttaacaa ttgaagaaac tgcagataat ttcattcgaa
     1681 ccccctgctc tctcatttct tgagtaggtt gaataaaagt acgacctaca tatttattct
     1741 taatcagacc catttcataa ggaatattag ctgcttctgc atatccgatt gcagcagaaa
     1801 tagaagaatc aggaacacca ataactaaat cagcttctac tggtgcttct ttaaataatt
     1861 gtttaccagc aagttttcta gttgtatgaa catttattcc atctaaatta ctatctggtc
     1921 gtgaaaaata aatatattcc attgcacata ttttatcagt aacatcattt gtataaagct
     1981 ttgatttgat ttgaccgtct ttgactctaa cgatttcacc aggttctaaa tcacgaacaa
     2041 attttgcacc aacaacttca aatgcacacg tttctgatga aaatacatat gccccattag
     2101 gcaattgacc aatagaaaca ggtcgcaaac catacttatc tcttgctaca tacaaagcat
     2161 tctcaatcaa aa
//
LOCUS       DAOMTA010000552         2171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2442_length_2171_cov_2.364839, whole genome shotgun sequence.
ACCESSION   DAOMTA010000552 DAOMTA010000000
VERSION     DAOMTA010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_2171_cov_2.364839"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..899)
                     /gene="deoC"
                     /locus_tag="PLK68_12775"
     CDS             complement(189..899)
                     /gene="deoC"
                     /locus_tag="PLK68_12775"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM91317.1"
                     /translation="MKKITKKANEMNLKELASYIDYSVLKPEFTEQEIIDLTKDGVKL
                     NCATICINPGYMDLCEPYVKGTDTMLCPVCDFPFGTSSTESKVKQIEIVAKYDSVKEI
                     DIVANFGMIKSGKWEEVLADIKACTEAAHKYGREIKVIFETDALNELQIRKMCHICIE
                     AGADFVKTSTGFLTGHEAHGASLEIIKVMMEECGDKIKIKGSGCIRTREHFLQLIDMG
                     IDRMGVGYRSVSVVLGLD"
     gene            complement(1073..1843)
                     /locus_tag="PLK68_12780"
     CDS             complement(1073..1843)
                     /locus_tag="PLK68_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM91318.1"
                     /translation="MMIASERFLRIVNTVNERGFISTKELSENLDVTETTIRRDCEEL
                     EKQGLLIRVHGGAKSIEQKTILSNKDEKQMSERITNNKEKDLVCKKAASFIRDGDCIF
                     LDGGTSIVPMLKYIKGKNVKIVTHSTLIANGFNDFESELFMIGGKYIPEYNMSVGPIT
                     LSDLEKFNFDYAFLSCAGIDIDRKLVYTAEMDTMAVKQKAMDLAVKKYLLIDSSKLSV
                     KGFCSFISSDDFDAVICNKDAHMNIDDIPVNYILTNED"
BASE COUNT          684 a          409 c          198 g          880 t
ORIGIN      
        1 gttagcttta attccttttg gtacaagtac attaaaatat caatacaaga taaagatcca
       61 cctggatgtc cagatttagc ttcatatatc attttaataa tactttttct agcttcatta
      121 gccattttat ttaacatcgt ttacaccgcc cccttctctt cttagctttt gtaaacactt
      181 ttgattagct agtctaaccc taacacaaca gatactgatc tatatccaac tcccatacga
      241 tcaattccca tatcaatcaa ttgtaaaaaa tgttctcttg ttcgaataca tccacttcct
      301 ttaatcttaa ttttatcacc acattcttcc atcattactt tgattatttc caaactcgct
      361 ccatgagctt catgtcccgt taaaaatcca gtacttgttt taacaaaatc agcacccgct
      421 tctatacaaa tatgacacat ttttcttatt tgtaactcat ttaaagcatc agtctcaaaa
      481 ataactttaa tttctcttcc atatttatgg gccgcttcgg tacaggcttt aatatccgct
      541 aaaacttctt cccactttcc acttttgatc attccaaagt tagcgactat atcaatttct
      601 ttaaccgaat cgtatttagc cacaatttca atttgtttaa ctttggattc tgttgagctt
      661 gttccaaatg gaaaatcaca tactggacat agcattgtgt ctgttccttt aacataaggc
      721 tcacataaat ccatataacc aggattaatg caaatagttg cacaatttaa ctttactcca
      781 tcttttgtta gatcgataat ttcctgttca gtaaattctg gttttaaaac tgaatagtca
      841 atataagatg ctaactcctt taaattcatt tcatttgcct tttttgttat ttttttcatt
      901 tttctaatag ataactattt atttttgttt gtttttgttc gttatctttc ctccttaatt
      961 aaaaaataac acataaacaa acatttgtaa acatatattt gtttattttt gttcgttttt
     1021 gtttttttgt ataaaaaaac atatcaaaca aactagttca atatgtcttt atttaatctt
     1081 catttgttaa aatataattt actggaatat catcaatatt catatgagca tccttattac
     1141 agataactgc atcaaaatca tcactgctaa taaagctaca aaaaccttta acacttaact
     1201 ttgatgaatc aattaataaa tacttcttta ctgctaagtc cattgctttt tgttttactg
     1261 ccattgtatc catttcagct gtatagacta attttctatc tatatctatc ccggcacaac
     1321 ttaagaaagc ataatcaaaa ttaaactttt ctaagtcact taaagtaatt ggaccaactg
     1381 acatattgta ttctggaata tatttaccac caatcataaa taattcagat tcaaaatcat
     1441 taaatccatt tgcaatcaag gtactatgtg ttactatttt aacatttttt ccttttatat
     1501 attttagcat cggtacaata cttgttccac catctaaaaa tatacaatct ccatccctga
     1561 taaatgatgc tgctttttta catactaaat ctttctcttt attattagta attctttcgc
     1621 tcatttgttt ttcatcttta tttgaaagaa ttgttttttg ctcaatactt tttgctccgc
     1681 catgaaccct tatcagcagt ccttgttttt caagttcttc acaatctctt cttatcgttg
     1741 tttctgttac atctaagttt tcagaaagtt cttttgttga aataaaccca cgttcattaa
     1801 ctgtatttac tattcttaaa aacctctcac ttgcaatcat catactcacc tcttcttttc
     1861 attatactta aatgccattt ttattcaatt attttaaaaa gataatcttt ttcattatat
     1921 aattactttt tataaaatta tatgatttgt taactataac tttaaacaat atctaagatt
     1981 tgctaaatca aactgttcta taaataaacc ttgatttaat caaggtttat tagtcaaaat
     2041 tattcaccaa cgtatgataa cacttatttt attgattttt aatggtttct catccataaa
     2101 tcactatatt tacacctatt atttctattt aaaagatttt ttgatgtcaa atcttttaga
     2161 aaaaaagaaa a
//
LOCUS       DAOMTA010000553         2170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2446_length_2170_cov_2.505437, whole genome shotgun sequence.
ACCESSION   DAOMTA010000553 DAOMTA010000000
VERSION     DAOMTA010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_2170_cov_2.505437"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..>2170)
                     /locus_tag="PLK68_12785"
     CDS             complement(163..>2170)
                     /locus_tag="PLK68_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM91319.1"
                     /translation="TEQLESTAALPVKTFKVVGIYQSTYLDQASYYNSFYTANSSSII
                     SSKMYLTLNHVDNDIFDNGSNTAQSLGIDSGDISYHQNLLYYYGISNNDGFNQAMMTA
                     VGIVVVIIMIGSIGLIYNAFAISLNERSRYLGTLSSIGATKRQKKQSVYFEGLVVGII
                     AIILGVIAGVGGMAVTFKVINPILANLGQEIGFPLAISFKGILTAVICAIITIFISSI
                     IPAYHASKISPIAAITNQQDTKIKVRNIKTSFLTRRIFGFEGDLAMKNIKRNKNRYRI
                     TLFSLIISSILFLTASGFTYYLKESYDMTSININYDGYININSQNQELINELAHLKNT
                     TQIAVAKETSLNLETSLDNINPELLSFLTENEQNYGNTYFLDLNLVTYNQEYLDSIPN
                     LNKELQNQILINKENNIKLNRKYKQTDILKENLDHLIGTTFNNNGDEIEITLNKIKYT
                     NQYLLGQTPHETHYSLNLLVSNDTFNQLINQYQLNSNNTNIYYTSNNNEAVLKEIKEI
                     TDNYPEESLYSFNLSEDIKQANQLILIINIFTYGFIILIGLISIANIINTISTSMALR
                     TKEFSMLKSIGMTPRAFNKMIYFESLFYGIKTLIYSLPLGFIIMYFLYENLAAIFERS
                     FSVPINSFIIITIAIFVIVFTTLAFSSQKIKKLNIIDGLKNDNN"
BASE COUNT          737 a          289 c          351 g          793 t
ORIGIN      
        1 acatgccttt cacgcatgca ttcacgggtt cgaatcccgt acaggtcacc atttgtaagt
       61 tagtccagga tatttatctt ggatttttat tttgtttaaa aaataactga taaaagaaaa
      121 agaacctaac ataaaattga actaggttct tttaacaatt aattagttat tatcattttt
      181 taaaccgtca atgatattta atttcttaat tttttgactg ctgaaagcaa gggtggtaaa
      241 aacaattaca aaaattgcaa tagttataat gataaaacta ttgattggaa cactgaatga
      301 ccgctcaaat attgcagcta gattttcata taaaaagtac atgatgataa agcctagagg
      361 caaactgtaa attagagttt taatgccata gaatagactt tcaaaataaa tcatcttatt
      421 aaatgctctt ggtgtcattc cgatagattt taacattgaa aattcttttg ttcttaatgc
      481 catactagta gaaatcgtat tgatgatgtt tgcgatactg attaaaccga ttaaaatgat
      541 aaagccatat gtgaagatgt taatgattaa aataagctgg tttgcctgct tgatatcttc
      601 acttaggtta aagctgtata gtgattcttc aggataatta tcggtaattt ctttgatttc
      661 ttttaaaact gcttcattat tattactcgt ataataaata ttagtattat ttgagtttaa
      721 ttgatattga ttaattaatt ggttaaaagt atcattgctg actaaaagat ttagtgagta
      781 gtgagtttcg tgaggagttt gcccaagtaa atactgatta gtatatttaa ttttatttaa
      841 agtaatttca atttcatcac cattgttatt aaatgtagtt ccgattaagt gatcaagatt
      901 ttcttttaag atatcagttt gcttatactt ccgatttaat ttaatattat tttctttgtt
      961 gattagaatt tgattttgaa gttctttatt taaattaggg atactatcta aatattcttg
     1021 attataagta actaaattaa gatcaaggaa ataagtattt ccatagtttt gttcattttc
     1081 tgttagaaat gataataatt caggattaat attatctaaa ctagtttcta aatttaatga
     1141 tgtttcttta gcaacagcaa tttgagtcgt attttttaga tgggctaatt cgttaatcag
     1201 ttcttgattt tgtgaattga tattaatata gccatcatag ttgatattga ttgaagtcat
     1261 atcatatgat tctttaagat aataagtaaa tccagaagcg gttaaaaaga ggatagaact
     1321 aatgattaat gaaaataggg tgattcgata acggttttta ttacgcttaa tatttttcat
     1381 cgctaaatca ccttcaaagc caaatattcg gcgagtaagg aaacttgttt taatatttcg
     1441 caccttgatt ttagtatctt gttgattagt gattgctgca atcggcgaaa tcttactagc
     1501 gtgataagct gggataatac tggagataaa aattgtgata attgcgcaaa taacagctgt
     1561 caagatacct ttaaatgaaa ttgctaaagg aaatccgatt tcttgtccaa gattagctaa
     1621 gattggattg atgactttaa aggtaacagc cattcctcca actcctgcta tcacacctaa
     1681 tataatagca ataatgccaa ctactaagcc ttcaaaataa acagattgtt ttttttgtcg
     1741 ttttgttgct ccgatacttg ataaagttcc aagataacga ctgcgttcat ttaaggaaat
     1801 tgcaaaagca ttataaatca aaccaataga accaatcatg ataatgacaa caacaatgcc
     1861 aacggctgtc atcattgctt ggttaaaacc atcattatta ctgattccgt aataataaag
     1921 aagattttga tgataactaa tatctccact gtctattcct aatgattgtg cggtatttga
     1981 gccgttatca aaaatatcat tgtcgacatg attaagggtt aaatacattt tgctgcttat
     2041 aatgctactg ctgttagcgg tataaaagga attataatac gatgcttgat cgaggtaagt
     2101 agattgataa ataccgacaa ctttaaatgt tttgactggt agggcagcag tagattctag
     2161 ttgttcggtt
//
LOCUS       DAOMTA010000554         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2454_length_2165_cov_2.324645, whole genome shotgun sequence.
ACCESSION   DAOMTA010000554 DAOMTA010000000
VERSION     DAOMTA010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2454_length_2165_cov_2.324645"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1975)
                     /gene="gyrB"
                     /locus_tag="PLK68_12790"
     CDS             complement(50..1975)
                     /gene="gyrB"
                     /locus_tag="PLK68_12790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRM91320.1"
                     /translation="MSEEKVEHSYDESDIQVLEGLEAVRKRPGMYIGTTSIRGLHHLV
                     WEIVDNSIDEALAGYASHIRVILKDDDVVQVIDDGRGMPTGTHAKTGISTVETIFTVL
                     HAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVNVHRDGHEYYQRFENGGKPAGELK
                     IIGDSDITGTIVTFKADKIIFKEGTVYDYDTLRQRIRELAFLNKGLRLSLEDQRNGVD
                     RKHEYYYEGGIKEYVAYINKNKTPIHEEIIYVEDMQQEITIEVGMQYNDGYQSNIYSF
                     CNNINTHEGGTHEEGFRLALTRVINNYAKSKGLLKKDEEALSGDDVREGLTAIISVKH
                     PDPQYEGQTKTKLGNAEVRKIASNIISASLDKFLLENPDTAKIIVDKAIVAARARMAA
                     KKAREMTRRKNVLEVSNLPGKLADCSSKDASECEIFIVEGDSAGGSAKMGRDRRIQAI
                     LPLRGKILNVEKSRLEKILGNAEIRSMITAFGTGIGEEFNLDKLRYHKIVIMTDADVD
                     GGHIRILMLTFLYRYLRPLVEGGFVYAAQPPLYLLKHGKDEYYCYSDEELDDLKTKIG
                     EGAKYSIQRYKGLGEMNAEQLWETTMDPEKRILNQIDLDEAMEADMIFDMLMGEKVEP
                     RREFIQENAKYVTDLDI"
     gene            complement(1975..>2165)
                     /locus_tag="PLK68_12795"
     CDS             complement(1975..>2165)
                     /locus_tag="PLK68_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRM91321.1"
                     /translation="EYPVLLLDDVLSELDEARKNMLLDILNQKIQTFITTTSIDGINH
                     QIVEKAKKIYIKGGKEAT"
BASE COUNT          647 a          467 c          261 g          790 t
ORIGIN      
        1 agttttactt ttctaattcc ccgttcttcc attatttatc tcctttatct taaatatcta
       61 aatcagtaac gtatttagcg ttttcttgaa taaattcacg tcttggttca actttttcac
      121 ccattaacat atcaaaaatc atatccgctt ccattgcttc gtctaaatca atttgattta
      181 aaattcgttt ttcaggatcc atcgttgttt cccaaagttg ttctgcattc atttctccaa
      241 gacctttata acgttgaata ctatatttag caccttcacc aatcttagtt ttcaaatcat
      301 ctagttcttc atcactataa caataatatt catctttacc atgtttcaat aaataaagtg
      361 gtggttgagc cgcataaaca aagccacctt caactaacgg tcttaaataa cgatacaaga
      421 atgttaacat taagattctg atgtgcccac catcaacatc ggcatcggtc ataattacaa
      481 ttttatgata tcttaattta tctagattaa actcttctcc gattcctgta ccaaacgctg
      541 taatcataga acgaatttca gcatttccaa ggattttttc taatctcgat ttttcaacgt
      601 ttaaaatttt tccacgtaat ggtagaattg cctgaattcg acgatcacgt cccattttgg
      661 cactaccccc agcggaatcc ccttcgacaa taaagatttc acattcacta gcatctttac
      721 ttgaacagtc agctaattta cctggcaagt ttgaaacttc aagaacattt tttcttcttg
      781 tcatttctcg agctttttta gctgccattc ttgctcttgc tgcaacaata gctttatcaa
      841 caataatctt agctgtatca ggattttcta ataaaaattt atctaatgat gcgctaataa
      901 tattgctggc aatttttcga acttcagcgt tacctaattt tgttttagtt tgtccttcat
      961 attggggatc aggatgttta accgaaataa tcgctgttaa accttcacga acatcgtcac
     1021 cagataaagc ttcttcatct ttctttagca gtcctttact tttagcataa ttattaataa
     1081 ctcgtgttaa agctaaacgg aaaccttctt catgagttcc cccttcatga gtattgatat
     1141 tattacagaa ggaataaata tttgattgat aaccatcatt atattgcatt cctacttcaa
     1201 ttgtaatttc ttgctgcata tcttcaacat agattatttc ttcatgaatc ggtgttttat
     1261 ttttattaat ataggcaaca tattctttaa ttccgccctc ataataatat tcatgttttc
     1321 gatccacacc gttacgttga tcttctaacg ataaacgtaa tcccttattt aaaaatgcta
     1381 actctctaat tctttgtctg agtgtatcat aatcataaac agtaccctct ttaaaaatta
     1441 ttttatcagc cttaaaagta acaattgttc ctgttatatc agagtcacca ataatcttta
     1501 attcacctgc tggtttccca ccattttcaa aacgctgata atattcatgt ccatcacgat
     1561 gaacatttac ttctaaccat tctgaaagcg catttacaac tgaggctcca accccatgaa
     1621 gtccaccaga tactttgtat ccgccaccac caaattttcc accagcatgt aatactgtaa
     1681 aaattgtttc aactgttgag attccagtct tagcatgagt accggttggc attcctcgac
     1741 cgtcatcaat tacttgaaca acatcatcat cctttaaaat tacgcgaata tgactagcat
     1801 atccagctaa ggcctcgtca atcgagttat caacaatttc ccaaactaaa tgatgtaatc
     1861 ccctaattga agttgtccca atatacattc ctggtctttt acgaaccgct tcaagtcctt
     1921 ctaagacttg aatatcggat tcatcataac tatgttctac tttttcttct gacattatgt
     1981 agcctccttg ccacccttaa tatagatttt ttttgccttt tcaacaattt gatgattaat
     2041 tccatcaatt gaagttgttg taataaaagt ttgaattttc tgattcaaaa tatctaataa
     2101 catattttta cgcgcttcat ctaactcaga taaaacatcg tctaatagta aaacaggata
     2161 ttctc
//
LOCUS       DAOMTA010000555         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2464_length_2161_cov_1.944919, whole genome shotgun sequence.
ACCESSION   DAOMTA010000555 DAOMTA010000000
VERSION     DAOMTA010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_2161_cov_1.944919"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLK68_12800"
     CDS             <1..560
                     /locus_tag="PLK68_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536636.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRM91322.1"
                     /translation="ATKELLKTTPGLEIINNILIPALDTVGKQYEKNIIFLPQLIQSA
                     ETSKIAFGIIKDTFKDTAATKGPIIMATVHGDIHDIGKNIVKVVLESYGYKVIDLGKD
                     VPPETVVEAFHKHHPKAIGLSALMTTTVVSMAKTIELLKQIDNICPIFVGGAVLTADY
                     AKEINADYYSKDAMEAVELLNKIIK"
     gene            complement(567..>2161)
                     /locus_tag="PLK68_12805"
     CDS             complement(567..>2161)
                     /locus_tag="PLK68_12805"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM91323.1"
                     /translation="VKTYLYNRKIELLETCSEETNQLLNYFTLCSDGSISTVAGKEVA
                     VGDPTETALVKASLEKGFTKDGLAELYPRFNELAFDSERKMMTVFVKHEDKIISITKG
                     GPDVIFARCNNLDIDEVSVVNETMSNEALRVLALGIRYWDEEPQEFTSEMIENNLTFI
                     GMVGMIDPPREEAKQAVAEAKSAGVRTIMITGDHVITASAIARSLGILESGQKAIMGS
                     ELAKMSDQELAEHIEEYSVYARVAPEHKVRIVNAWKSKGKVVAMTGDGVNDSPALKAA
                     DIGCAMGITGTDVAKGAASMILTDDNFATIITSIREGRGIFDNIKKDVQFLLSSNIGE
                     VLTIFGASLISLLTPHNFGVPLLPIHLLWVNLITDSLPAFALGMEPTEPTVMHRKPRQ
                     KDEGFFSHGLGFTIAWQGLMIGTLTLIAYAIGNNQDHTVGMTMAFITLCGCQLVHSFN
                     VKSSASILNRTLFNNPYLWGALAAGLLLQVIILAIPELSAIFKLQPLNIVQWGICVGL
                     CLSTTVICELVKFFDRRKNNPS"
BASE COUNT          731 a          428 c          307 g          695 t
ORIGIN      
        1 atgctacaaa agaattattg aaaacaacac ctggtttaga aataattaat aatattctca
       61 ttcctgcttt agataccgtt ggtaaacagt atgaaaaaaa tataatcttt ttacctcaat
      121 tgattcaatc tgctgaaaca tcaaaaattg ctttcgggat aattaaagat acttttaaag
      181 atactgctgc tacaaaagga ccaatcatta tggcaacagt tcatggagat attcatgata
      241 ttggaaaaaa tattgttaaa gtagtattgg agagctatgg ctataaggta atcgatttag
      301 gtaaagatgt tccaccagaa actgttgttg aagctttcca taagcatcat cctaaggcaa
      361 tcggtttaag tgcattaatg actaccaccg tagtttcaat ggcaaaaact atcgaactat
      421 taaaacaaat cgataatata tgccctattt ttgtaggtgg tgctgttctt actgctgatt
      481 atgctaaaga aattaatgct gattattatt ctaaagatgc tatggaagca gtagaactac
      541 ttaataagat aataaaataa tccatcttaa gatggattat tttttcgtct atcaaaaaat
      601 tttacaagtt cacaaatcac agtagttgat aaacaaagtc ctacacaaat tccccattga
      661 acaatgttta atggttgcaa tttaaaaatc gcacttaatt caggaattgc taaaataatc
      721 acttgtaaca ataaccctgc tgctaatgct ccccataaat aaggattatt aaatagtgtt
      781 cgatttaaaa tcgaagcact acttttaaca ttaaaagaat gcactaactg acaaccacat
      841 aaagtaataa aagccattgt catcccaact gtatgatctt ggttattgcc tattgcataa
      901 gcaatcaacg ttagcgttcc gatcattaat ccttgccaag caatagtaaa acctaaacca
      961 tgcgagaaaa atccttcatc tttttgacgt ggtttacgat gcattactgt tggttcagtc
     1021 ggttccattc ctaatgcaaa tgctggtaaa ctatcagtaa ttagattcac ccataacaaa
     1081 tggatcggta acaatggcac cccaaaatta tgtggtgtta acaatgagat caacgaagct
     1141 ccaaaaattg tcaatacttc acctatatta cttgataata agaattgaac atcttttttg
     1201 atattatcaa aaataccgcg accttcacga attgatgtaa taattgtcgc aaaattatca
     1261 tctgtcaaaa tcatgcttgc agcacctttt gcaacatcag ttccagttat tcccatcgcg
     1321 caaccaatat ctgctgcttt caaagctggt gaatcattaa ctccatcacc ggtcatagct
     1381 actactttac ctttggattt ccaagcatta acaattcgta ctttatgttc tggtgcaact
     1441 cgtgcataaa cagaatactc ttcaatatgc tcagctagtt cttgatctga catttttgcc
     1501 aactcacttc ccatgattgc tttttgacca gattctaaaa ttccaagact tcgagcaatt
     1561 gcacttgcgg taataacatg gtcaccggta atcataatcg ttctgacccc agctgattta
     1621 gcttctgcca ctgcctgttt agcttcttca cgtgggggat caatcattcc aaccatccca
     1681 ataaaagtta aattattttc aatcatttca ctggtgaatt cttgtggttc ttcatcccaa
     1741 tatctaattc ctaaagctaa aactctcagt gcttcatttg acattgtttc attaactacg
     1801 ctaacctcat caatgtctaa attattgcat cgtgcaaaaa taacatcagg tcccccttta
     1861 gtaatcgaaa taatcttatc ttcatgttta acaaaaactg tcatcatttt acgctcagaa
     1921 tcaaaggcca gctcattaaa tcgaggatat aattcggcta aaccatcttt ggtaaatcct
     1981 ttttctaaac tagctttaac aagagcagtt tccgtaggat caccaactgc aacttccttt
     2041 cctgcaacag tagaaatact accatcacta cacaaagtaa aataattcaa taattgatta
     2101 gtctcttcac tacaagtttc taataattca attttgcgat tataaagata tgtttttaca
     2161 a
//
LOCUS       DAOMTA010000556         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2469_length_2159_cov_1.892586, whole genome shotgun sequence.
ACCESSION   DAOMTA010000556 DAOMTA010000000
VERSION     DAOMTA010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2469_length_2159_cov_1.892586"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLK68_12810"
     CDS             complement(<1..764)
                     /locus_tag="PLK68_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRM91324.1"
                     /translation="MNYNEEYQRWVNCENLDPSLKAELASMNEKEKEDAFYMYLEFGT
                     AGMRGILGAGTNRMNIYTIRKANVGFAKYVLGLPEGKERGVAIGYDNRHMSYKFAIES
                     AKVLATYGIKSYIFESLRPTPELSYAVRYLKCAGGIMITASHNPKEYNGYKVYDDTGC
                     QLIPEWGDQVVAYVNEVKDELAVEVISDEEAYPYITWIGEEVDEAYYQEVMAIEINPG
                     MDKKDFKIVFSPQHGTSNLPVRNCLSRLGYNVIPVLA"
     gene            complement(842..1339)
                     /locus_tag="PLK68_12815"
     CDS             complement(842..1339)
                     /locus_tag="PLK68_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRM91325.1"
                     /translation="MIQCYYGNGKGKTTAAVGQALRMAGADKKVLFLQFLKDGDSSEI
                     KMLKKCGIKVLYAKMPQMFIDMHDPEMIKLVSRLEDELFEQIDESYEGIVLDEILDAI
                     ALNLLNEGKVYDCLVSLKETHEVILTGRQPSHKLKPILDYSSEIKKHKHPYDKGIKAR
                     KGIEF"
     gene            complement(1342..1821)
                     /gene="ybaK"
                     /locus_tag="PLK68_12820"
     CDS             complement(1342..1821)
                     /gene="ybaK"
                     /locus_tag="PLK68_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRM91326.1"
                     /translation="MAKKIKTNALRLLDQAKIDYEIKEYEYDEDHLSGEHIVAQVEIP
                     AKDIFKTLVLKGNRGFLVCCIPVLEEIDLKKLAKLSNNKSVAMIHMKDLLATTGYIRG
                     GCSPVGMKKKFDTYFDCSINECDKIALSAGKRGYQMIVEAKQLLAYLDAVVGDVIRR"
     gene            complement(1905..>2159)
                     /locus_tag="PLK68_12825"
     CDS             complement(1905..>2159)
                     /locus_tag="PLK68_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HRM91327.1"
                     /translation="SFVVSDPVGLHGRPATILVNQASKFTSNIKLVYNGKEVNLKSIM
                     GVMSLGVPTKATVEIVAEGDDEKDVIASIAKVIKEQKVAE"
BASE COUNT          665 a          454 c          239 g          801 t
ORIGIN      
        1 gctaaaaccg gaataacatt atagcctaaa cgactcaaac aatttctaac tggtagattt
       61 gaagtaccgt gttgtggtga gaaaacaatt ttgaaatctt ttttatccat accgggatta
      121 atttcaattg ccattacttc ttgataatat gcttcatcta cttcttcacc gatccatgta
      181 atatatggat aagcttcttc atcgctaatt acttctactg ccaattcatc tttaacttca
      241 tttacatatg caactacttg atctccccat tcaggaatta attggcatcc agtatcatca
      301 tatactttat aaccgttgta ttctttaggg ttatgacttg cagtgatcat aatcccacca
      361 gcacatttta aataacgtac tgcataagat aattctggag ttggtcttaa actttcaaaa
      421 atataagatt taattccata agtagctaaa actttcgcac tttcaattgc aaatttgtaa
      481 gacatgtggc ggttatcata accaattgcg actccacgtt cttttccttc tggtaaacct
      541 aatacatatt tagcaaagcc aacattagcc tttctaattg tatagatgtt cattcggtta
      601 gtaccagccc ctaaaattcc tcgcattcct gcagttccaa actctaggta catatagaat
      661 gcatcctctt tttccttttc attcatactt gctaactctg cttttagtga tggatctaaa
      721 ttttcacaat ttacccatct ttgatattct tcattgtagt tcatgttttt cacctcttaa
      781 tttaattata acaaacctgt aaaaaaataa ctacttgttt tataaatgaa attactacaa
      841 attagaactc aattccttta cgcgctttta ttcctttatc atatggatgc ttatgtttct
      901 taatttcact cgaataatct agaatcggtt ttaatttatg actaggctga cgccctgtta
      961 aaattacctc gtgagtttct tttaagctta ccaagcaatc atacactttc ccttcattta
     1021 ataaatttaa agcaattgca tcaagaattt catctaaaac aattccttca taactttcat
     1081 ctatttgttc aaataactca tcttctaaac gactcaccaa ttttatcatc tctggatcat
     1141 gcatatcaat aaacatctga ggcattttag catacaacac tttaattcca cactttttca
     1201 gcatctttat ttcactgctg tcaccgtctt tcaaaaattg tagaaataat acttttttat
     1261 ctgccccagc cattcttaat gcttgtccta ctgcagcagt agtttttcct ttaccattac
     1321 cataataaca ttgaatcatc gctatctcct tatcacatca ccaactaccg catctaaata
     1381 tgctagtaac tgctttgctt ctacaatcat ttgatatcct ctttttccag cactgagagc
     1441 aattttatca cactcattaa ttgagcaatc aaaataagta tcaaattttt tcttcattcc
     1501 cactggacta cagcctccac gaatatatcc ggtcgttgct aataaatcct tcatatgaat
     1561 cattgctaca cttttattat tagataactt tgccaatttt tttaaatcta tttcctctaa
     1621 taccggtata cagcatacta aaaaaccacg gtttcctttt aaaaccagtg tcttaaaaat
     1681 atctttagcc ggtatttcaa cttgtgcaac aatatgttca ccacttaaat gatcttcatc
     1741 atattcatat tcctttattt cgtaatcaat ctttgcttga tctaataaac gtaaagcatt
     1801 agttttaatt tttttagcca taaaaacctc ctaacaacat tattatctta atccaaacat
     1861 aaattttaag caaataaaaa gaagtccgag gacttctaat tttcttattc agctactttt
     1921 tgttctttaa taacttttgc aattgaagca attacatctt tttcgtcatc accttcagct
     1981 acgatttcta ctgtagcttt agtaggaaca cctaaagaca taactcccat gattgatttt
     2041 aagtttactt ctttaccatt gtatactaat ttaatattgc ttgtaaactt actagcttgg
     2101 ttaactaaga tagtagctgg tcttccatgt aatcctacag gatcagatac tacgaaaga
//
LOCUS       DAOMTA010000557         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2475_length_2157_cov_1.770219, whole genome shotgun sequence.
ACCESSION   DAOMTA010000557 DAOMTA010000000
VERSION     DAOMTA010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2475_length_2157_cov_1.770219"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..931)
                     /locus_tag="PLK68_12830"
     CDS             complement(110..931)
                     /locus_tag="PLK68_12830"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRM91328.1"
                     /translation="MKDIEEEYLTFVVRHYKAHKYDPNKAFKKFKKITGSNNNIKHKT
                     LRIISVAASIAIIIGVLIVYNYYEKTKNLIVVASAGQCKTVKLEDGSKIILSPYSSFT
                     YNIVEMEKGDRSVKLIGKAFFSIHHDEKHPFSVDGKVGKVKILGTVFQVDEQNKNTST
                     VYVVSGKVYFSGLNSNKGVLLTKGMKATLTGNKRKPLIVSSGNINQTAWATGVFKFNN
                     TPINEALGDISEYYHVNIIASDTTKCISGEFEATNMNDVVDLLESTLGIKIQINK"
     gene            complement(975..1484)
                     /locus_tag="PLK68_12835"
     CDS             complement(975..1484)
                     /locus_tag="PLK68_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRM91329.1"
                     /translation="MYNFNDLFNSYYRSLCIYAIHLINDDDSVEDIVMECYIKLWNRL
                     NNGEHIDDVKNYLFIAVRNACYDLNRKNCSSNDFIQIEQVKDVIQLNCDDDRSYREAR
                     LWKAIDSLPKKCREIFLMSKRDELTYVEIANELNLSVKTVEAQIGKAYKILRGKAMEI
                     YFLSLSLFL"
     gene            complement(1541..>2157)
                     /locus_tag="PLK68_12840"
     CDS             complement(1541..>2157)
                     /locus_tag="PLK68_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91330.1"
                     /translation="EEFFILIGFELVIASNSIAQNEMNIGLKKMEKQVVDNKKFDLKY
                     EEWLQNEQIKNYVYLNGEDYLWNQSFNNWQSHQVDFPLKMRDSTNLVNLDPYKNIGHY
                     ISRNLILTGNYRKLLMNKNAFIFDAKLNFKVNDWFSLGIGGSYSSKMRSLLAYDGDNS
                     NYYAYLRFKINNFWYIEPQFKRYLNVSTFKWQNSFGISSIWHFK"
BASE COUNT          707 a          401 c          263 g          786 t
ORIGIN      
        1 ttttgtattt gttccatttc ctgtttaacc gtaatctgct gagcaaatcc agattgactc
       61 acaaatatag aaataaataa tattaataag tatctcatat tgccattcgc tatttattta
      121 tttggatttt tatgcctagt gtactttcca acaaatccac aacatcattc atatttgttg
      181 cttcaaattc accagaaatg cattttgtcg tatcactggc aatgatattc acatggtaat
      241 attctgatat atctcccaaa gcctcattga tgggcgtatt gttaaactta aacactcctg
      301 tagcccaggc tgtctggtta atatttccag aagagacaat taaaggtttt cttttgtttc
      361 ctgtcagcgt tgccttcatt cctttcgtca acaatacacc cttattacta tttagtccgg
      421 aaaagtatac ttttccactt actacataga cggtagaagt atttttattc tgttcatcta
      481 cctgaaaaac agtacccaga atcttaactt ttccaacttt accatctaca gaaaatggat
      541 gcttttcatc atgatggatt gaaaaaaaag ccttgcctat aagttttacg gaacgatctc
      601 ctttttccat ctcaacaata ttatatgtaa aactagaata tggagataaa attattttac
      661 taccgtcttc aagcttaaca gtcttacact gtcctgcaga agcaactaca atcaggtttt
      721 tagtcttttc ataataatta taaacaataa ggactcctat aattatagct atagaagctg
      781 cgactgatat aatacgcaaa gtcttgtgtt ttatattatt atttgatcct gttatttttt
      841 taaacttttt aaaagcctta ttaggatcgt atttgtgtgc tttgtaatgt ctaactacaa
      901 acgtcaaata ctcttcttca atatctttca tatgcatatt tttattatat agactcatat
      961 tagatgaaat acccttacaa aaacaaggat aaacttaaaa aatagatttc cattgcctta
     1021 cctctcagaa ttttatatgc cttacctatt tgagcctcca ctgttttaac agatagattt
     1081 aattcatttg ctatttctac ataagtaagt tcatcgcgct tactcattaa gaaaatctct
     1141 ctacattttt tgggtaatga gtcaatagcc ttccaaagac gggcctctct atatgacctg
     1201 tcatcgtcac aatttagctg aatcacatcc ttaacttgct ctatctggat aaagtcatta
     1261 gacgaacaat tctttctatt taaatcatag caagcatttc tgaccgcaat gaaaagatag
     1321 tttttaacat catcaatatg ttccccatta tttaatctat tccacaattt tatataacac
     1381 tccataacaa tatcttcaac agaatcatca tcattaatga gatgaattgc atatatacac
     1441 aaagaacgat agtacgaatt aaataagtca ttgaaattat acatattttg cttaggattt
     1501 atcaaacttt tatatctata tatgctcgat tatacattaa ctatttgaaa tgccatattg
     1561 acgatattcc aaatgaattt tgccacttaa acgtagaaac atttaagtat cttttgaatt
     1621 gaggctctat ataccaaaaa ttgttgatct taaacctaag ataagcataa taattgctgt
     1681 tatcaccatc atacgccaat agacttctca tttttgaaga atatgacccg ccaataccta
     1741 atgaaaacca atcatttacc ttaaaattca atttagcatc aaatatgaat gcatttttgt
     1801 tcatcaatag tttccggtaa ttacctgtta aaattagatt tctacttata taatgaccta
     1861 tatttttata aggatctaaa tttacgagat tggtggagtc acgcattttc agaggaaaat
     1921 ccacttgatg agattgccaa ttgttaaagc tttgattcca aagatagtct tcgccattca
     1981 gataaacata atttttaatc tgttcatttt gaagccattc ttcatatttc aagtcaaact
     2041 ttttattatc cacgacctgt ttttccattt ttttcaagcc gatattcatt tcattttgcg
     2101 caatactatt ggatgcaata acaagttcaa agccaatcaa tatgaaaaac tcttcat
//
LOCUS       DAOMTA010000558         2150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2489_length_2150_cov_2.172315, whole genome shotgun sequence.
ACCESSION   DAOMTA010000558 DAOMTA010000000
VERSION     DAOMTA010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2489_length_2150_cov_2.172315"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLK68_12845"
     CDS             <1..371
                     /locus_tag="PLK68_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRM91331.1"
                     /translation="YAVENIIAEKIETTLDRGIYNTRMRDLFDVYNLLTQKDISIDMN
                     VLIDSFVNVSKSRNTLNNIYDYEELISELSESAVFEENFNRFKKIKEIEDVSLEDIFT
                     VFKDVIEKIDVNQFINSRSR"
     gene            394..912
                     /locus_tag="PLK68_12850"
     CDS             394..912
                     /locus_tag="PLK68_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91332.1"
                     /translation="MIIDRKYAVIKYFVIGFILLIIIIASIFYFTGNEDSKTKYKYKY
                     LNEDQITFASDEQVDDYKIMLERYLEKNKYSDVETVKFYNRTFKEDNYVYFYCLLDDE
                     FKTLLECKYDKSEEKFLNYFEWVGDKYDDSTEAPASKITYLEIVDKESYESKKFDEEI
                     ENREPDENIDSD"
     gene            1182..1436
                     /locus_tag="PLK68_12855"
     CDS             1182..1436
                     /locus_tag="PLK68_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91333.1"
                     /translation="MNKKLINRIKKLEEKKEQLLTQTNEIDVELKKLYELKKEDENLQ
                     KKRMCFEEKLDNILVKAKKPDEKSMQRNLCEDGENVNDIQ"
     gene            1423..>2150
                     /locus_tag="PLK68_12860"
     CDS             1423..>2150
                     /locus_tag="PLK68_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91334.1"
                     /translation="MTFNKITYSLYQKVKYLIDAAYPNIDENVIGNYKKINIVLSKKT
                     LKQNEKYEDRKCIIYNLYRQESELSNSLLICLAHHIDYLVRGETKNDSEFNKIYIHIL
                     HTAINEKMVKYDELKRTDDYKNKKLIQKALDSYWESNKKFDTVYLEIYNCYEIKSDLK
                     RDGFVYNEYYQCWQKEVKTNNISTQKDYCFNLKSDLIFNIREKNHIIFTLYGMICVTG
                     NTYFAKDILKKNKYFFKENCWQKK"
BASE COUNT          938 a          175 c          355 g          682 t
ORIGIN      
        1 cttatgcagt tgaaaatata attgcagaaa aaatcgaaac aactcttgac cgaggtatat
       61 ataataccag aatgagggat ttatttgatg tttataatct tttaacacaa aaagatatta
      121 gtatagatat gaatgtatta atagattcat ttgtaaatgt ttccaaaagt agaaatacat
      181 tgaataatat ttatgattac gaggagctta tcagtgaatt aagtgagtca gctgtctttg
      241 aagaaaattt caacagattt aaaaaaatta aggaaattga agatgtctct ctcgaagaca
      301 ttttcacagt ttttaaggat gttattgaaa aaattgatgt caatcaattt ataaattcac
      361 gttctagata gataaattag tataggagaa gatatgatta ttgatagaaa atatgctgta
      421 attaaatatt ttgttattgg attcatactg ttgataatta tcattgctag tatattttat
      481 tttactggca atgaggacag taaaacaaaa tataagtata aatatcttaa tgaggatcaa
      541 attacatttg catctgatga acaagttgat gattacaaaa taatgttaga aaggtattta
      601 gaaaaaaata aatatagtga tgttgaaaca gtaaagtttt ataacaggac ttttaaggaa
      661 gataattatg tttattttta ttgtttgctg gatgatgaat tcaaaacatt gttagaatgt
      721 aaatatgata aaagtgagga aaaattttta aattattttg aatgggttgg agataagtat
      781 gatgatagta ctgaggcacc cgcaagtaaa attacttatc tggaaattgt tgataaagaa
      841 tcatatgaaa gtaaaaaatt tgatgaagaa atagaaaata gggaacctga tgaaaatata
      901 gactcagatt aaaatagcaa ttatgtatag atcctggtaa aaattaccag gatttttaga
      961 ttggggtaga aagtatgttg acgttctatt ttaagagaaa aaagaatttg atttgaaatt
     1021 tcaaaatgga tttataagta tttttctaaa catagtatat atgatctatt tgtaatagag
     1081 acaggtgata accgaataag taaaaaataa aaagaataga tattaagtaa ttaaataatc
     1141 gtctttcaaa taactgtaaa agatgataat gtggaggaaa tatgaataag aaattaataa
     1201 acagaattaa gaaactggaa gaaaaaaaag aacaattatt aacacaaaca aatgaaattg
     1261 atgtggaatt aaaaaaatta tatgaactga aaaaagaaga tgagaatcta cagaaaaaga
     1321 gaatgtgctt tgaagaaaag ctggacaata ttttggtaaa agcaaaaaag ccggatgaaa
     1381 agtcaatgca aagaaatcta tgtgaagatg gggaaaatgt aaatgacatt caataaaata
     1441 acatattcat tgtatcaaaa agttaaatac ctgattgatg ccgcttatcc aaatattgac
     1501 gaaaatgtta ttggaaacta taaaaaaata aatatagttt tatcaaaaaa aacattaaaa
     1561 caaaatgaga aatatgaaga cagaaagtgt attatttata atttatatcg ccaggaaagt
     1621 gaactgtcaa attctctttt aatttgttta gcgcaccata ttgattattt agtgagaggt
     1681 gaaacaaaaa atgatagtga atttaataaa atttacatcc atatattaca cactgcgatt
     1741 aatgaaaaaa tggtcaaata tgatgaatta aagcgaacag atgattataa aaataaaaag
     1801 ttgattcaaa aagccctgga ttcatactgg gaaagtaata aaaaatttga tacagtttat
     1861 ttagaaattt ataattgtta tgaaataaaa agtgatttaa aaagagatgg atttgtgtac
     1921 aatgaatatt atcagtgttg gcaaaaagaa gtaaaaacaa ataatatttc cacgcaaaaa
     1981 gattattgtt ttaatttaaa gagtgattta atttttaata ttcgagaaaa aaatcatatt
     2041 atttttacac tttatggaat gatttgtgtt acgggaaata catattttgc aaaagatatt
     2101 ttaaagaaaa ataaatactt tttcaaagaa aactgttggc aaaagaaaat
//
LOCUS       DAOMTA010000559         2143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2507_length_2143_cov_2.264368, whole genome shotgun sequence.
ACCESSION   DAOMTA010000559 DAOMTA010000000
VERSION     DAOMTA010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_2143_cov_2.264368"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1011)
                     /locus_tag="PLK68_12865"
     CDS             complement(136..1011)
                     /locus_tag="PLK68_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM91335.1"
                     /translation="MEIRTLQYFLTIAREESISGAAEYLHVTQPTLSRQMKELEEELG
                     KQLFIRGKRRITLTDEGMILRKRAEEILGLVERAEAEVKANEELLTGDIYLGCGESEG
                     MRPIAKTIATMLEKYPHVKFHLHSGKAEEVMEKIDAGVLDFGIVIEPVDLNKYDYLRL
                     PYSNVWGILMKRDSTLASLDKITPDDLRGKPLLCSNQGMVKNELAGWIGGNQRKLNIV
                     GTYNLLFNASLIVEEGNLYALCLDKIFNSNNSSLCFRPLEPKLEANMLIIWKKYQLFT
                     KSAETFLNMIKDNIT"
     gene            complement(1100..2047)
                     /locus_tag="PLK68_12870"
     CDS             complement(1100..2047)
                     /locus_tag="PLK68_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRM91336.1"
                     /translation="MLEFYKTFGTETKKIDKPEPGSWISAIAPTEEEKNYLIEEMGIL
                     PEFVKSSLDAEESSHIDYDEDYNQTLVIVDYPSAEEVEDGYDKNMLQYTTLPLGIVIM
                     KGYVVTISLYENLNIDDMAQGRIKGVNTDLKTRFLLLLLLRISQRYLIYLRQIDRISS
                     RTEQRLHKSMQNKELIQMLGLEKSLVYFSTSLKTDEITLNKIMRGKAIKLYDEDQDLL
                     DDVLIEIHQAIEMCNIYSNILSGTMDAFASVISNNLNIVMKVLTVITIVMAIPNIIFS
                     FYGMNVAGLPFPQWWFPTGLAIVACIIATIIFIKKDMFH"
BASE COUNT          675 a          386 c          292 g          790 t
ORIGIN      
        1 tgcagatgtt ccatttttag atgtatgttc attacctaaa gatgaaacac catttgatat
       61 aagtgacatt attgcgaaac gaaaaaataa tttattgtaa aaagagaaat aacaatttta
      121 gttctctttt tatctttatg taatattgtc ctttatcata tttaaaaatg tttcagcact
      181 tttggtaaag agttggtatt ttttccaaat tattagcata ttcgcttcta attttggttc
      241 aagtggtcta aagcataatg aactattatt agaattaaaa attttatcta agcataaagc
      301 atataaattt ccttcttcaa ctattaaaga tgcattaaat aataaattat aagttcctac
      361 aatatttaat ttcctttgat ttccaccaat ccatccagct aattcatttt ttaccatccc
      421 ttgatttgaa caaagaagag gttttccacg tagatcatca ggggttattt tgtcaagtga
      481 tgctagagta ctatcccgct tcattaaaat cccccaaaca ttgctataag gaagccttaa
      541 ataatcgtac ttatttaaat caacaggctc aataacgatt ccaaaatcta gaactccagc
      601 atcaattttt tccattacct cttcagcttt tccactatgc aaatgaaatt ttacatgagg
      661 gtatttttcc aacatcgttg caattgtctt ggcaatcgga cgcattcctt cactctcacc
      721 acatcctaga taaatatcac ctgttaatag ttcttcatta gctttgactt cagcttctgc
      781 cctttcaaca agcccaagaa tttcttctgc ccgttttctt aagatcatgc cttcatcagt
      841 caaagtaata cgacgttttc cccggataaa taattgtttt cctaattctt cttctaactc
      901 tttcatttgt cttgataatg ttggctgcgt tacatgtaga tattcagctg ccccagaaat
      961 actttcttcc cttgcgatgg taagaaaata ttgtagtgtt ctaatttcca ttaatatcac
     1021 cccatagtta tattatagta catttatgtt taacaagtat aaaaaactag attataaata
     1081 catataatct agaatttatt taatgaaaca tatctttttt gatgaaaata attgtggcga
     1141 taatacaggc aactattgct agtcccgttg ggaaccacca ttgtggaaat ggtaaacctg
     1201 caacattcat tccataaaaa ctgaaaataa tatttggaat agccataacg atcgtaataa
     1261 ctgttaatac tttcataaca atattcaggt tattggaaat aactgaagca aatgcatcca
     1321 ttgttcctga taaaatattt gaatagatat tacacatttc aattgcctga tggatttcaa
     1381 tcaaaacgtc atctaatagg tcttgatctt catcatataa tttaatagct ttcccacgca
     1441 taattttatt taatgttatt tcgtccgttt ttaatgaggt agaaaaatat actaatgatt
     1501 tttctagtcc taacatttga atcaattctt tattttgcat tgatttgtgt aatctttgtt
     1561 cagttcgaga agagatccga tcaatttggc gcagataaat taaatatcgt tgagaaattc
     1621 gtaataataa tagtaataaa aatcttgttt ttaaatcagt attgacacct ttgatccgac
     1681 cttgtgccat atcatcgata tttaagtttt catataagga aatggtaaca acgtatcctt
     1741 tcatgatcac aatacctaag ggcaatgtcg tatattgtaa catattttta tcgtaaccat
     1801 cttctacttc ttcagcacta ggatagtcaa cgattactag agtctgatta taatcttcgt
     1861 catagtcgat atgcgagctt tcttcagcat ctaatgaaga tttaacaaat tccggtagaa
     1921 ttcccatttc ttcaatcaaa tagttttttt cttcttcagt tggagcaata gcgctgatcc
     1981 agctgcctgg ttctggttta tcgatctttt ttgtttcagt tccaaaagtt ttgtaaaatt
     2041 ctaacataat aacctccact tacaggaggt tcatgtttat gcctcctgtt tctctatttt
     2101 attttctttt tcttagcgca caatcgctcg ggcacagggg aca
//
LOCUS       DAOMTA010000560         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2509_length_2142_cov_2.654528, whole genome shotgun sequence.
ACCESSION   DAOMTA010000560 DAOMTA010000000
VERSION     DAOMTA010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_2142_cov_2.654528"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..>728)
                     /locus_tag="PLK68_12875"
     CDS             complement(50..>728)
                     /locus_tag="PLK68_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM91337.1"
                     /translation="VIASGNQYYRLYQKFLPLSEQMYFIAENGCYIAEGATELYCNTI
                     THDNVELIKTALAPYDNLFMIMCGRKGAYVLSRDKHFESLVRLHYCNYQFVDSFDHID
                     DEIMKISINDPEEQIEKYLQALEPHLPVAVKVVTAGNMWMDIHNRDINKGVGMRFLQA
                     IYEIEPDECMAFGDQMNDYELLQQVKYGYAMDNAVLPIKEIAYGTTRSNDEQGVLIKI
                     KEVLDLA"
     assembly_gap    729..738
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<739..816)
                     /locus_tag="PLK68_12880"
     CDS             complement(<739..816)
                     /locus_tag="PLK68_12880"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91338.1"
                     /translation="MDGTFLDSDKRFDPEFIDIFYKLKEK"
     gene            complement(968..1366)
                     /locus_tag="PLK68_12885"
     CDS             complement(968..1366)
                     /locus_tag="PLK68_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM91339.1"
                     /translation="MNKRQMQLIIGILVIIIIVLLVYRNHRQETVYQRISASEAQKIM
                     DEESNIIIIDVRTVDEYKTGHIKNAICIPNELISNKEIAELPDKSQEILVYCRSGSRS
                     RQAANKLIKLGYENVIDFGGIIDWDGEVVK"
     gene            complement(1359..1820)
                     /gene="bcp"
                     /locus_tag="PLK68_12890"
     CDS             complement(1359..1820)
                     /gene="bcp"
                     /locus_tag="PLK68_12890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRM91340.1"
                     /translation="MLEVGSIAPDFTLLDQNGDSVSLSNFVGKKIILYFYSKDNTPGC
                     TKQACGYAQNYPRFKEKDTIIIGISKDTVVSHKKFEEKYQLPFILLANPELDVLQAYD
                     VWKEKNMYGRMVMGVVRTTYLINEEGIIEKAFTKVKAADDAERMLREIESE"
     gene            complement(2039..>2142)
                     /locus_tag="PLK68_12895"
     CDS             complement(2039..>2142)
                     /locus_tag="PLK68_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HRM91341.1"
                     /translation="KAIFNNPNIEKAVDDFNQDWYSVYGENKGICDL"
BASE COUNT          699 a          424 c          223 g          786 t
ORIGIN      
        1 ttaataaaag gtagtttacc ttttttcgtg taaaaaaagc ctagcaatac taggcaagat
       61 ccaatacttc tttaatttta attaagactc cctgttcatc attggatcta gttgtaccat
      121 aagcaatctc cttaattggc agaactgcat tatccatggc ataaccatat ttcacttgct
      181 gtaataactc ataatcattc atttgatccc caaaagccat acattcatca ggttctattt
      241 catagattgc ttgtaaaaaa cgcattccaa cacctttgtt aatatcacga ttatgaatat
      301 ccatccacat atttcctgct gttacaacct taactgcgac tggtaaatgc ggctccaatg
      361 cttgcagata tttttctatc tgttcttctg gatcattaat agaaatcttc atgatttcat
      421 catcgatatg atcgaatgaa tcaacaaatt gataattaca ataatgtaat ctcaccaatg
      481 attcaaaatg cttatcacgg cttaagacat aagccccttt ccgaccacac atgatcataa
      541 ataaattatc atatggggct aatgctgttt tgatcaattc aacattatcg tgtgtaattg
      601 tattacaata caattctgtc gctccctctg cgatgtaaca accattttca gcaataaaat
      661 acatttgttc acttaatggt aaaaattttt gatataatcg ataatactga ttaccactag
      721 caattacann nnnnnnnnct tttcctttag tttataaaat atatcaataa attccggatc
      781 aaaacgttta tctgaatcca aaaatgttcc atccatatct gttgcaatta attttatcat
      841 catagcctcc ttatcaggat gtagtatagc ataatctcta tttagataaa agaaaaaggt
      901 gccaaaatat agtgatcatt tctagttgct cactcaactt ttaaagttca ctacaaatag
      961 caccctctta tttaactact tcaccatccc aatcaatgat tccaccaaaa tcaataacat
     1021 tttcataacc aagtttaatc aatttgtttg cagcctgacg acttctactt ccactgcgac
     1081 aatatactaa tatttcttgt gatttatctg gtaactctgc aatttcttta ttagaaatta
     1141 attcattggg aatacagata gcatttttta tatgcccggt cttatattca tctactgttc
     1201 ttacatcaat aatgataata ttactttctt catccataat cttttgagct tcactcgccg
     1261 aaatcctttg ataaacagtt tcttggcgat gattacgata cactaataaa acaataataa
     1321 tgataactaa aataccaata attaattgca tctgtctttt attcactctc aatctctctt
     1381 aacattcgct cagcatcatc tgctgcttta acctttgtaa atgccttttc tataatacct
     1441 tcttcattaa ttaaatatgt agttcgcact acacccataa ccattcgacc atacatgttt
     1501 ttttctttcc aaacatcata agcttgtaaa acatctaatt ctggattagc taataaaata
     1561 aatggaagct gatatttttc ttcaaatttt ttatgtgata caaccgtatc tttactaatc
     1621 ccaataatta ttgtgtcttt ttctttgaat cgaggatagt tttgagcata tccacaagct
     1681 tgtttagtgc agcctggcgt attatcttta ctataaaaat ataaaattat tttctttcca
     1741 acaaaattac tcaatgaaac gctgtcacca ttttgatcta ataaggtaaa atctggagca
     1801 atacttccca cttctaacat cttttatttc tctcctttat ttttctataa taaatagcat
     1861 acctattttt gataccaaat gcaaatatta tgacttataa atattatacg attatatcaa
     1921 cattttatac tatttatcat cagttatccg agcattcttt caccactgat ttttaaactt
     1981 cttaaaccac tcatttttct ttaataaaaa gaaaaagtgg aatcctccac catttctttt
     2041 ataaatcaca aattccttta ttttctccat aaacactata ccagtcttga ttaaaatcat
     2101 ctacagcttt ttcaatattt ggattattaa aaatcgcttt ta
//
LOCUS       DAOMTA010000561         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2510_length_2142_cov_2.241974, whole genome shotgun sequence.
ACCESSION   DAOMTA010000561 DAOMTA010000000
VERSION     DAOMTA010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2510_length_2142_cov_2.241974"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLK68_12900"
     CDS             complement(<1..672)
                     /locus_tag="PLK68_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRM91342.1"
                     /translation="MAGVVVVGSQWGDEGKGKITDYLAQKADIVARYQGGNNAGHTIE
                     FNGQKFALRLIPSGIFSGNDVILGNGMVINPKALLEEMKYLNDANISTDKIMISDRAH
                     VILPYHLEIDEIQETRRGANNIGTTKKGIGPTYVDKYERVGIRMGEFVDEELFKERLE
                     EALASKKAQYPELTCSAEEIFEEYKEYAKIIAPMVCDTGVVLDECFQTGKNVLFEGAQ
                     GTMLDI"
     gene            843..1256
                     /locus_tag="PLK68_12905"
     CDS             843..1256
                     /locus_tag="PLK68_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM91343.1"
                     /translation="MYIKLEQYKIFNEAATTLSFSIAARNLFISQSAISQTISSIEKE
                     LQTQLFIRNSKGVSLTKEGKLLHQQIDKALALITGVENQLSNYHELKDGQLIIGAGDT
                     FSEYFLTNYIVKFKQLYPGVKIKVINRTSLETREF"
     gene            1246..1722
                     /locus_tag="PLK68_12910"
     CDS             1246..1722
                     /locus_tag="PLK68_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRM91344.1"
                     /translation="MNSDQIDLGFLNLPIKDDSLVIKECFQVHDIFVSKKPDNHIYTF
                     NQLADKPLILLEKSSNTRNRIDNYFANKGLLLKPTIELGAHNLLLDFARAGLGISCVI
                     KEFSQELLDQGILHEIKTSQPLPKRSIGYAYPKRRTQSVATNKFIELIANDPTLHI"
     gene            complement(1757..2008)
                     /locus_tag="PLK68_12915"
     CDS             complement(1757..2008)
                     /locus_tag="PLK68_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase to dihydroxyacetone kinase"
                     /protein_id="HRM91345.1"
                     /translation="MLEFRYDTQLLIEGKNLDEDVINDYITEHFKGDCLLAVGDDELI
                     KIHFHTNEPWQVLEYCASLGEIYDIVVEDMERQSRGLKG"
BASE COUNT          738 a          411 c          258 g          735 t
ORIGIN      
        1 aatatctaac attgtccctt gagccccttc aaataataca tttttaccag tttgaaaaca
       61 ttcatctaaa acaacaccag tatcacaaac cattggtgca atgattttag catattcttt
      121 atattcttca aagatttcct cggcactaca tgtaagttca ggatattgcg cttttttaga
      181 agccaaagct tcttctaatc tttctttaaa taattcctca tcaacgaatt cacccatacg
      241 gattcccacc cgttcatatt tgtcgacata tgtaggtcca attccttttt tagtcgttcc
      301 gatattatta gctccacgcc tagtttcttg aatttcatcg atttctaaat gataaggtaa
      361 gataacatga gcccgatcac taatcatgat cttatcagta ctaatattag catcatttaa
      421 atatttcatt tcttcaagta aagctttagg attaattacc attccgttac ctaaaataac
      481 atcattacca ctaaaaatcc ctgaaggaat caaacgtaaa gcaaactttt gaccattaaa
      541 ttcaattgta tgcccggcat tattaccacc ttgatatcta gcaacgatat ctgctttttg
      601 agccaggtaa tcagtaattt tacctttacc ctcatcgccc cattgacttc ctactacaac
      661 aactccagcc attaaattca cacctttctt atttactatt acattatata caataaacta
      721 acaaaatgca tcaataaata tacattaata taatagtttg actagtttac agaataatta
      781 tattatagtc ttagctatta gtaaaattaa tatattataa ttatgatata aggagaactt
      841 atatgtatat caaactagaa caatacaaaa tattcaatga agctgctacg accctatctt
      901 tttcaatcgc agctagaaat ctttttattt ctcaatctgc aatttcccaa acaatcagca
      961 gtattgaaaa agaactacag actcaattat ttattcgcaa ttctaaaggt gttagtttaa
     1021 ctaaagaggg taaattatta catcagcaaa ttgacaaagc tttagcattg attaccggag
     1081 tagaaaacca gttatcaaat tatcatgaat taaaagatgg tcaattaatt attggagccg
     1141 gtgatacttt tagtgagtat tttttaacta attatatcgt taaattcaaa caactatatc
     1201 ctggggttaa aatcaaagta atcaaccgaa ccagtctaga aacccgtgaa ttctgatcaa
     1261 atagatctcg gttttttaaa tcttcccatc aaagatgatt ctttagtaat taaagaatgc
     1321 ttccaagttc atgatatttt tgtttctaaa aagcctgata atcatattta caccttcaat
     1381 caattagctg ataagcccct aattctttta gaaaagtcat caaatacccg taatcgtatc
     1441 gataattact ttgctaataa gggactatta ttaaagccaa caattgaact tggagctcat
     1501 aatttattgt tggactttgc cagagcagga ctaggaattt cttgtgtcat taaagaattc
     1561 tcccaagaat tacttgatca agggattctt catgaaataa aaacatcaca accattgcct
     1621 aaacgcagta tcggttatgc ttatccaaaa cgacgaactc aatctgttgc tacaaacaaa
     1681 tttattgaac taattgctaa tgatccaact ttacatatct aaaaacgtca ctgattaatt
     1741 tagtgacgtt ttttatttat ccttttaacc ctcttgactg acgttccata tcctcaacaa
     1801 cgatatcata gatttcacca agtgatgcac aatattccaa cacttgccac ggttcgttag
     1861 tatggaaatg aatcttgatc aactcatcat caccaaccgc taacaaacaa tctcctttaa
     1921 aatgttcagt aatgtaatca ttgataacat cttcatcgag atttttacct tcaatcaata
     1981 gttgagtatc atatctaaat tctagcatat tattctccta attataacct ggttgagtta
     2041 aaaatttata aactcgctca ataatttttg attctaactt attatatgat aaagttggat
     2101 cttttaataa aggtatttcg aaaacatctt ttcgtccttg at
//
LOCUS       DAOMTA010000562         2140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2516_length_2140_cov_1.819185, whole genome shotgun sequence.
ACCESSION   DAOMTA010000562 DAOMTA010000000
VERSION     DAOMTA010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_2140_cov_1.819185"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..639
                     /locus_tag="PLK68_12920"
     CDS             55..639
                     /locus_tag="PLK68_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91346.1"
                     /translation="MSRKSDFINGLNKFTSDINTTNQKLDNILKSSDYTPEYKSKAKL
                     TAHQAKINIGRDLGESLHTILDEAINAYSVKLDKANKERMLNQGYQLGLQNLVNAIGS
                     NALSNDDLQSMYEVYKDDVIAVKSINAAVGKSGNAYLELLPLPDNEKTITRLNKLKSN
                     VDNYIMTPNNDSDLSVIGMTQFINDSLNVDMYSL"
     gene            657..806
                     /locus_tag="PLK68_12925"
     CDS             657..806
                     /locus_tag="PLK68_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91347.1"
                     /translation="MERDNDFQEKLNKKIDKIFADKEQERKESKRKAEEKELKDQIYR
                     NMKGI"
     gene            811..1344
                     /locus_tag="PLK68_12930"
     CDS             811..1344
                     /locus_tag="PLK68_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91348.1"
                     /translation="MDDIQKVIENCKRQLNEKLETSDMYLRQVKLSDQLIQYKTDFRN
                     VVDQLNRAENDNDYIKVVELEQKKETTKKAIQIVEERLSKIDLSLALDHKDVKIIIND
                     FTKAIGTIISEQEVSYRKVANELLNLNRQSKEYNNDIYMFENYLHDKHHYLNALNLRA
                     KCSASLESELQKIVNDI"
     assembly_gap    1574..1583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1584..1785
                     /locus_tag="PLK68_12935"
     CDS             <1584..1785
                     /locus_tag="PLK68_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91349.1"
                     /translation="EGVLCVIKLGAYKIKVRIQKSMHTKMIKQLRKVKIYTYRGKCYY
                     QLITIGLLTVSNGWVATKIQVA"
     gene            complement(1931..>2140)
                     /locus_tag="PLK68_12940"
     CDS             complement(1931..>2140)
                     /locus_tag="PLK68_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91350.1"
                     /translation="GWNAYAIESNTKCFIGVFGREPKNYEEVREWIEWVLSENDKTLI
                     VEPTTCKEIIKLADKKAKSITTYEY"
BASE COUNT          803 a          275 c          383 g          669 t
ORIGIN      
        1 gtatatagaa attaattaca tgatctaaaa aacaagaaaa gaggttaaat aaagatgagt
       61 agaaaaagtg attttataaa cggactgaat aagttcacaa gtgatattaa tacaactaat
      121 caaaagttgg acaatatctt aaagagcagt gattataccc ctgagtataa aagcaaggct
      181 aaattgaccg cacatcaggc taaaatcaat attggccgtg atttagggga atcactgcat
      241 actattcttg atgaggctat taatgcatat agtgttaaat tggataaggc caacaaagaa
      301 cgtatgttaa atcaggggta tcagctagga ctgcagaacc ttgttaatgc cattggcagt
      361 aatgcattat caaatgatga tcttcaaagt atgtatgagg tctataaaga tgatgttatt
      421 gccgtaaaat caattaatgc tgcagttggt aaatcgggta atgcatattt ggagctattg
      481 cccctacctg ataacgaaaa gactattaca aggttaaata aactgaaatc aaatgttgat
      541 aactatatca tgacccctaa taacgacagt gatttatctg ttattggtat gactcagttt
      601 atcaatgaca gtttaaacgt cgatatgtac agtttataaa attggaggaa tataaaatgg
      661 aaagagataa tgattttcaa gaaaaattaa ataaaaaaat agataagatt tttgctgata
      721 aagaacaaga acgtaaagaa tcaaaaagaa aagctgaaga aaaagaatta aaggatcaaa
      781 tttatagaaa tatgaaaggt atataatagt atggatgata ttcaaaaagt tattgaaaat
      841 tgtaagcgac aattaaatga gaaacttgaa actagcgaca tgtatttacg ccaggtaaaa
      901 ttaagtgatc agttaattca atataagact gattttagga atgttgtgga tcagcttaat
      961 agagctgaaa atgataatga ttatattaaa gttgttgaac tggaacaaaa gaaagaaact
     1021 actaaaaaag caatacaaat agtagaggag cgcttatcaa aaattgattt atcattagca
     1081 cttgatcaca aagatgttaa aattattatt aatgatttta ctaaagcgat tggcacaatt
     1141 attagtgaac aagaagtttc ttatcgtaaa gttgccaatg aattgttgaa tctaaatagg
     1201 caatctaaag aatataataa cgatatctac atgtttgaaa attatttaca tgataaacac
     1261 cattacctga atgctttgaa tttaagagct aaatgtagtg caagtttgga atctgaatta
     1321 caaaaaatag ttaatgatat ttagccctct atgggctttt tattatatcc ttttcttgct
     1381 taactcacgc ttaaactggc taaataaagg gttttagaag cgtttataga caacggatat
     1441 aaaaatatcc tgattagcct aaacgtcctc agacagtaag ctagttatgg ttaaaaatgc
     1501 atacggagca ttctaagtgt tatgttatat tgattgtgag tttattttat gattatgatg
     1561 ccgatttatg tatnnnnnnn nnntgagggt gtattatgtg ttataaaatt aggtgcatac
     1621 aaaataaaag tacgcataca aaaaagtatg cacacaaaaa tgataaaaca gttaagaaaa
     1681 gttaagattt atacgtatag gggaaaatgc tattatcagc tcataactat tggattgctt
     1741 acagtttcca acggttgggt tgcaaccaaa attcaggttg cataaaaggt tgcactcctt
     1801 tgatttaggg gtgcatttac aaaacgacaa aggtacaccc ctttttcaaa atgacaatta
     1861 tgcacacaaa aaaacacttg ctatgtattg gcgtacattg gtagagcaag tgcggtatta
     1921 tattatattt ttagtattcg taagttgtta ttgattttgc ttttttatcg gcaagtttaa
     1981 tgatttcttt acatgtagta ggctcaacta tgagtgtttt atcattttct gatagtaccc
     2041 attcaatcca ttctcgaact tcttcatagt tcttgggttc tcggccaaat acgccgataa
     2101 agcattttgt atttgattct atagcatagg cgttccaccc
//
LOCUS       DAOMTA010000563         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2519_length_2138_cov_2.249160, whole genome shotgun sequence.
ACCESSION   DAOMTA010000563 DAOMTA010000000
VERSION     DAOMTA010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2519_length_2138_cov_2.249160"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLK68_12945"
     CDS             <1..985
                     /locus_tag="PLK68_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91351.1"
                     /translation="SLIENILNYFVVNAMIKPIKELNCMIDILIKAGGFILIIMLGFA
                     LKTKGVCTREHGSFLSTIIMNITLPCSLLSSINNLEITPILLVALACGFLGNVITNLS
                     GYLIQKKESPMTRALSMINSSGYNIGTFTLPFVQSFFPSNLIGYVCLFDTGNALMCLG
                     GTYSAASTVVANEEKQSFKTVAKKLFSSIPFCTYIILFFLSLFHIAIPTQILTVTSIA
                     GNANAFLAMLMIGILLEIKLDLSQIRLIKKILLNRYAVTLGLSLFVYFILPIDLTVKK
                     MIILCLCSPISAVAPVFSNRLGSRSPVPSAINSLSIIISIFIMTILILFMV"
     gene            complement(1268..>2138)
                     /locus_tag="PLK68_12950"
     CDS             complement(1268..>2138)
                     /locus_tag="PLK68_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HRM91352.1"
                     /translation="KGLLVVMIAYIGIVATTLNAGATNQIDNYVETIDVKVQDGNQDQ
                     KDYLIRQASVSSVLDDLKISVNPQDILNLDLNYIVNKGDLIQITRVNQADIDEMNTVN
                     TTGLELFTTKVAQQGQNGQVKNTYRVTYENGNEVGRELIGSQVVSQATDTIIETGAVQ
                     EGAFFTGRLTTYGGDCAGGNGTSSTGIKLSPISGVQGSNSPKLTYNGRSYYCLAADPS
                     IPFGTIIEITNHNLSIESTAYGIVVDRGGAIKGNKIDIFNGTEAGKYFTGGTSKNTQF
                     KIISVGSGKNFWK"
BASE COUNT          642 a          387 c          285 g          824 t
ORIGIN      
        1 ttcactaatt gaaaatattt taaattattt tgtcgtaaat gctatgataa agccaataaa
       61 ggagttgaat tgtatgattg atattttaat taaggccggt ggctttattt taattattat
      121 gttaggattt gcactaaaga caaaaggggt ctgtacccgt gaacacggaa gttttttatc
      181 aacaatcatt atgaatataa cgcttccatg ctcattactt tcatcaatta ataaccttga
      241 gattaccccc atcctattag tagcccttgc ttgtggcttc ttaggcaatg ttattactaa
      301 cctttctggt tacttgattc aaaaaaaaga atcacccatg accagagctc tatcgatgat
      361 caactcatct ggctataata tcggaacttt tactttacca tttgttcaaa gtttctttcc
      421 atctaacttg attggttatg tttgtctttt tgataccggc aatgctttaa tgtgtttagg
      481 aggtacttat agtgctgcta gtacagttgt agctaatgaa gaaaaacaaa gctttaaaac
      541 tgttgctaaa aaattatttt catcaattcc tttttgcact tacatcattt tgtttttctt
      601 atctttattt catattgcaa ttcctacaca aattttaaca gttactagta ttgctggtaa
      661 tgctaatgct tttttagcaa tgttaatgat tggaatttta ttggaaataa aattagattt
      721 atcacaaatc agactgatta aaaaaatact tttaaaccgc tatgcagtta ctttggggtt
      781 atccttattt gtatatttta ttttaccaat tgatttaact gttaaaaaaa tgattatttt
      841 atgcttatgt tcgccaatat cagctgttgc cccagttttc tcaaatcgtt taggaagtcg
      901 ttccccagtt ccttcagcaa tcaactcttt aagtattatt attagtattt ttattatgac
      961 gattttaatc ttatttatgg tttaataaac cggtatttct ttatgtattg attaaattga
     1021 atgatttgtc gtatttattt aatacctgat acaaagggat tctcacaata cttcttacag
     1081 ccacatcaac aaggcgaacc cttttttcaa tatcatttgc taatcgctct tttaaacttt
     1141 tctcatctac taaaagcaaa atacgcttta cttcacaatc aataaaatct aatcattcta
     1201 aacacatatc aataattttt tgttcatgta tcacctaaca aaaaaactag tcattaattt
     1261 gactagttta tttccagaag ttttttccac ttcctactga gataatttta aattgggtat
     1321 tctttgatgt tccaccagta aaatatttac cagcttcagt cccattaaag atatcaatct
     1381 tgttaccttt aattgcacca ccacgatcta caacaatccc atatgcagtt gattcaatac
     1441 ttaaattatg attagtaatt tcaataattg ttccaaaagg aattgaagga tctgcagcta
     1501 agcaataata tgaacgtcca ttatatgtta atttaggact gtttgatcct tgtactccag
     1561 aaatcggact taatttaatg ccggttgatg aagttccatt tcctccagca cagtcgcctc
     1621 cataagtagt cagtcttcca gtaaagaatg ctccttcttg gactgcacct gtttcaatta
     1681 ttgtatcagt agcttgtgat accacctgac taccaattaa ttctcttcct acttcgttac
     1741 cattttcata agttacgcgg taagtgtttt tcacttgccc gttttgccct tgttgcgcaa
     1801 ctttcgtagt aaataactct aatccagttg tatttaccgt attcatctcg tctatatccg
     1861 cttgattaac tcgagtgatt tgtattaaat cacctttatt tacaatataa tttaaatcta
     1921 aatttaaaat atcttgagga tttacactga tttttaaatc atcaagaaca cttgatactg
     1981 acgcttgtct aattaaatag tccttttggt cttgatttcc atcttgaact tttacatcaa
     2041 ttgtttccac gtagttatca atttggtttg ttgctccagc atttaatgtt gtcgctacaa
     2101 ttccaatata ggcaatcatt actacaagta accctttc
//
LOCUS       DAOMTA010000564         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2520_length_2138_cov_2.026404, whole genome shotgun sequence.
ACCESSION   DAOMTA010000564 DAOMTA010000000
VERSION     DAOMTA010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2520_length_2138_cov_2.026404"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLK68_12955"
     CDS             complement(<1..1031)
                     /locus_tag="PLK68_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRM91353.1"
                     /translation="MQSLDKTHDSVENDFISILLHHTELLKSTVVERTYFDNEILRNI
                     FSLLKECETFDAVLFTDKGFEDIDFLMRVYDRFIFETSYYQMFKSYETRIINHYKTKC
                     LNELNLRLTKRQIDYNSYKEEFDKIDEIKPLSTKEHPTEKELIEAITSHNRIIDLGKF
                     NNLGKILKLESDDLVTVSAPPNFGKTPFLLNVFNECLNNKNNYCQYYNLEVNRNQIIK
                     RLIAIESKETINNVCSYKRSPKENISKAIKKYGSGDYYLNDEPMAWERMQAEIISHLK
                     KDKQNIVFIDYLGLIGLRNSSYNRTNYDKITYIMKELRKLCRKYNVLVFVASQCNRSA
                     LNNGVLTMY"
     gene            complement(1006..1824)
                     /locus_tag="PLK68_12960"
     CDS             complement(1006..1824)
                     /locus_tag="PLK68_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91354.1"
                     /translation="MAVKRMFSVDVVCSDNFIDMSHDAQLLYFHLSMNADNEGFVNNP
                     RTIIRTIGVDKKCLNQLIDSNFVIPFDSGVMVITHWRQNNNLDEKRMKKTTFLDEKNS
                     LIVENNIYIQVDCDTKLMSSMELHGTPKNSNKFHASIVENSIVESSKTKQNKNIKNRS
                     TETIPYQRIVDIYNTSCNRLLKVTKLTNNRKKVIRARYMEYEKDISIFETLFKKTNES
                     DFLCGINSEWRANFDWLLNQQNMVKVLEDRYINKHNQKNKESELVKNAIFGQDS"
     gene            complement(1915..>2138)
                     /locus_tag="PLK68_12965"
     CDS             complement(1915..>2138)
                     /locus_tag="PLK68_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91355.1"
                     /translation="VSMLREVGIIRATKTGRCYMFSQAEIQRFQRDYVGLDVSNKVKA
                     IEAYNAVESTKTATAPTVAKKATHQNYFQ"
BASE COUNT          621 a          369 c          269 g          879 t
ORIGIN      
        1 ctatacatgg ttaatacacc gttgtttaaa gcacttctat tgcattggct tgctacaaat
       61 accagtacgt tatactttct gcatagcttc cttagctctt tcatgatata tgttattttg
      121 tcatagtttg ttctgttata actgctattc cttaacccta ttaagcctaa atagtcaata
      181 aagactatgt tttgtttatc tttctttaag tggcttataa tctccgcttg cattctttcc
      241 catgccattg gttcatcatt taaataataa tcaccgctac catacttctt gattgctttg
      301 ctaatatttt ctttaggtga ccgtttataa ctgcaaacat tatttatcgt ttcttttgac
      361 tctatagcta taagtctttt gataatttgg tttctattaa cttcaaggtt gtaatattga
      421 cagtagttgt ttttattgtt taagcactca ttgaaaacat tcaatagaaa aggggtttta
      481 ccaaagttgg gtggtgctga taccgtaaca agatcatcac tttctagctt taatatcttg
      541 cctaaattat taaactttcc taagtctata atgcgattat ggcttgttat agcttctata
      601 agttcctttt ctgttggatg ttcctttgta cttaatggtt taatctcgtc tatcttgtca
      661 aattcttctt tgtagctatt ataatcaatc tgtcttttag taagtcttag attaagttca
      721 tttaaacatt tggttttgta atgattaatt atccttgttt cgtaactttt aaacatttgg
      781 taatatgaag tttcaaatat aaagcgatcg taaacacgca ttagaaagtc aatatcctca
      841 aaccctttat cagtgaatag aactgcatca aatgtttcac attccttaag aagtgaaaat
      901 atatttctta atatttcatt atcaaagtaa gttctttcta ctactgtact ttttaatagt
      961 tctgtatgat gaagcagaat gcttataaag tcattttcta cgctatcatg agtcttgtcc
     1021 aaagattgca tttttcacta actcactttc tttgtttttt tgattatgtt tatttatata
     1081 tctatcctct aataccttga ccatattttg ttgatttaac agccaatcaa aattagctct
     1141 ccattcactg ttaataccgc ataaaaagtc actttcattt gttttcttaa ataatgtttc
     1201 aaatatactt atatcttttt catattccat atatctagct ctaataactt ttttcctatt
     1261 atttgttaat tttgttactt ttaataatct attacatgaa gtgttatata tatctactat
     1321 tctttgataa ggaattgttt cagtagaacg atttttaata tttttatttt gttttgtttt
     1381 actactctct actatactat tctctactat actagcatgg aatttattgg aattctttgg
     1441 agttccatgt aattccatgg aactcatcaa tttagtatcg caatcaactt gaatatagat
     1501 attattttca actattaatg agttcttttc atcaagaaat gtagtttttt tcattctttt
     1561 ttcatctaaa ttattgtttt gtctccagtg tgttattacc ataaccccgc tatcaaaagg
     1621 gataacgaaa tttgaatcta ttaattgatt caaacatttt ttatcgacgc ctatagttct
     1681 aattatagtc ctaggattat tcacgaaacc ctcattatca gcattcatag ataaatgaaa
     1741 atacaataat tgtgcatcat gtgacatatc tataaaatta tcactacata caacatcaac
     1801 tgaaaacatt ctttttactg ccatttataa aaaccctttc tagcttataa aactgaccgt
     1861 acaaaatcag atttataagc attttgtttt aaatactcta atgtgataca atcactactg
     1921 aaagtaattt tggtgagttg ctttcttggc tactgttggc gcagtagccg tttttgtgct
     1981 ttcaactgca ttgtaggcct caatagcttt taccttgttg cttacatcta accccacata
     2041 gtcacgttga aaacgttgta tctcagcttg tgagaacata tagcatctac ctgttttagt
     2101 ggccctaatg ataccaactt ctctaagcat ggacacct
//
LOCUS       DAOMTA010000565         2137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2522_length_2137_cov_2.083573, whole genome shotgun sequence.
ACCESSION   DAOMTA010000565 DAOMTA010000000
VERSION     DAOMTA010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2522_length_2137_cov_2.083573"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(572..1237)
                     /locus_tag="PLK68_12970"
     CDS             complement(572..1237)
                     /locus_tag="PLK68_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91356.1"
                     /translation="MIEVKELNKKYGKTTVLSNINITFNAGHTYGVMGENGAGKSTLF
                     RCIMRLEGYDGKIEITKGLNVGYLPDTPYFYSLVTGMEFIEFCLKARGLKIDYNEIDA
                     VNDLFRLPLNRFPSSYSMGMRKRLVLMILILQHSDYYILDEPFNGLDLAGSILLKKWI
                     LRMKADGKMVVLSSHIISSLTDICDEITYIHAGNIVGTYRGLSAEEIEKDITTNFITD
                     TVF"
BASE COUNT          805 a          343 c          284 g          705 t
ORIGIN      
        1 ctagagaatt tgaagatatc tttaatttca gtgtatatac atatagcatg atatctgcgg
       61 tagagccaag aaacatagtg actccaattg ttatgtatgt agaatttata aatacatatc
      121 cgcatataac aggtataact cctaatatta tgcttggtga gataactgct atttgatatt
      181 gccaacattt tactggcgaa gaacaatagc aaccagggct gtataagtat ttctttacaa
      241 attctaattt aacattcttc ttgctgccac taaaaagaac aaaaaataga taatgaatta
      301 tttcatggag aaaaacacat gtaatcaata tacatattag aaaaggaaaa ctaacgaatg
      361 tatcatcttt ttctttcggt aaatatatgt tcgcatacgg ctttacgaca gttagatata
      421 gcagtaccaa acaaataaaa gaggtataat atacggtctt cttttttatt acgaatgttt
      481 tcatatttta cttattatat tactattacg tgcacaaata ttgcgctttc taaaatattt
      541 ccacttaaac acactcattc attatatata tttaaaacac agtatctgtt atgaaattag
      601 tcgtaatatc tttttctatt tcttcagcac ttagccctct atacgttcca acaatattac
      661 ctgcatgaat ataggttatc tcatcacaaa tatctgttaa tgatgaaatg atatgcgatg
      721 agagaacgac cattttgcca tctgccttca ttcgcaaaat ccatttcttt aataaaatag
      781 agcctgccaa atccagccca ttaaaaggtt cgtctaaaat atagtaatca ctatgctgaa
      841 gaattaatat catcaataca agtctttttc tcattcccat agaataacta ctaggaaatc
      901 gatttaacgg taaacggaaa agatcattga cagcatctat ttcgttataa tctattttca
      961 atccacgcgc tttgagacaa aattcaataa actccatacc tgtaaccaat gaatagaagt
     1021 atggcgtatc aggtaaatat cccacattca aaccttttgt aatttctatt ttaccatcat
     1081 acccctctaa cctcattatg catctgaaaa gagtgctttt acctgcacca ttttcgccca
     1141 tcacaccata ggtatgtcct gcattaaagg taatgtttat attagaaagt acggtagtct
     1201 ttccatattt tttatttagt tccttaactt ctatcatgta agtattggtt caattaattt
     1261 attagtttga aatattaata ttaacgttaa taatacttga attatcaaaa agtatccttg
     1321 gaccaatgat gcacatccaa aaaagaaaat ggctgtgagt atgaaaaact gcaataactg
     1381 attcttcgaa aaaacatatc tcagacattc tgtttgtata aagaagagaa cagtaataaa
     1441 atatacttta acgcaccata aaaagtcagt tgtattaata ttatttactg aatagagaat
     1501 tagaaaagga aataatatta ctgctgcatt acataatgca atctttatat tcattagcat
     1561 aaaatgtttc agtgagcaat agttataaag ataatattgt tttataggct ggcaaattaa
     1621 atttgctatc atgtaaacta taatcaacat tgatacttgc aatatccgaa tattaccata
     1681 cacaattccc atgattgaag caaagaacag caacagatag aaaataaaaa gtatacgaaa
     1741 gctactctgg taatggtagc tcccttttat aagaaatgga ttcgtgtgca tttgaaaatt
     1801 aattctaata cgtggtatca aaggaagaat gctagcaatt aaaatacata aggagatgtc
     1861 ataataataa ccattaaaaa tagatataag tataaaaggt atcgttaaaa tcaaatattc
     1921 taatataaaa aggttcttgt acttttcacc aaccaaagac ttcaataatg ttctgtctcc
     1981 cctcttagtg tgtaaagaaa tgactataca aaaatatata cttgctatat ataactcagg
     2041 caatttgaaa agaatatata gaaagcaaac aataaataga aataataacg atgatgttat
     2101 gtatcctaat tgttttaata aacctaagac cattttt
//
LOCUS       DAOMTA010000566         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2527_length_2134_cov_2.317941, whole genome shotgun sequence.
ACCESSION   DAOMTA010000566 DAOMTA010000000
VERSION     DAOMTA010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_2134_cov_2.317941"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..345)
                     /locus_tag="PLK68_12975"
     CDS             complement(7..345)
                     /locus_tag="PLK68_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91357.1"
                     /translation="MTKNTMIFIFLNMIYLLIWHATNKIRSTKAGKELDNGFEFYNSL
                     NTSDKENYWKEDTKILNLFFVLFIISMDISVILLFNENNLWIFSLVAGLIISSVVAII
                     LSINLKKKYK"
     gene            complement(430..1338)
                     /locus_tag="PLK68_12980"
     CDS             complement(430..1338)
                     /locus_tag="PLK68_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRM91358.1"
                     /translation="MKRMLVTGGTTFVSKYVAEYFVNAGYEVFVLNRNSKPQVQGVKL
                     IQGDRHNLGGVLKDTFFDVVADITAYNDKDIIDFVKELGSFDQYIMISSSAVYPEYGV
                     QPFLEESEKSENKFWGSYGTDKIAAEKALLERVKDAYILRPPYLYGPMNNVYREAFVF
                     DCAMADRKFYLPQDGSMKLQFFHVKDLCRLMEVIIKEKPEEHILNVGNVETVSIKDWV
                     TKCYESLGKIPEFVNVYEEIEQRNYFSFYNYEYYLDVSRQSKIYPETTSLEDGLRDSV
                     KWYLKHSAEVNKKPYFEYINENFVES"
     gene            1650..1886
                     /locus_tag="PLK68_12985"
     CDS             1650..1886
                     /locus_tag="PLK68_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6061 family protein"
                     /protein_id="HRM91359.1"
                     /translation="MRTIFAEYNPHRNSIDVYTSAGYMLRIDCWEAEKNLKTTPGSDC
                     ALTSLAVDEPLEYARLYLDGNLQMWVNAEDSLEI"
BASE COUNT          718 a          398 c          278 g          740 t
ORIGIN      
        1 attcacttat ttgtatttct tcttaagatt aatactcaat attattgcaa caactgatga
       61 aataattaat cctgcaacta atgaaaatat ccaaagatta ttttcattaa aaagaagaat
      121 aacggaaata tccatagata ttatgaaaag tacaaagaaa agatttaaaa ttttagtatc
      181 ttctttccaa taattttctt tatcactagt gtttaaacta ttgtaaaatt caaacccatt
      241 atctagttct ttaccagctt tagtgcttct tattttgttt gttgcatgcc aaataagtag
      301 gtatatcatg tttaaaaaaa taaaaatcat agtattcttt gtcatgtttg ttcctccaca
      361 aattccagtt tatctaacaa atcactaaat tgtaatttat agtattcttt ataacgattg
      421 ctattcaaat taactctcca caaaattttc atttatatat tcaaaatatg gtttcttatt
      481 tacctctgcg ctatgtttca agtaccattt aacactatct ctcaatccgt cttctaatga
      541 tgtggtttct ggatatattt tgctttgacg tgatacatcc aaatagtact catagttgta
      601 aaaactaaaa taatttctct gttcaatttc ctcataaaca tttacaaatt ccggtatttt
      661 tcctaagctt tcatagcatt ttgttaccca atctttaata gaaactgttt caacattacc
      721 aacatttaaa atatgttctt caggcttttc tttaattatt acctccatca acctgcataa
      781 atctttcaca tgaaaaaatt gtaatttcat actaccatct tgtggtaaat agaattttct
      841 gtctgccatg gcacaatcaa atacaaaagc ctctctatac acattattca ttggtccata
      901 taaataaggc ggtcttaata tatatgcatc tttcactctt tcaagcaatg ccttttcagc
      961 tgctatttta tcagttccat atgaacccca aaacttattt tcggatttct ctgattcttc
     1021 cagaaatggc tgaacaccat actctggata aacagcactc gaactaatca taatatactg
     1081 atcaaaagaa cctaattcct taacaaaatc aattatatct ttatcgttat aagccgttat
     1141 atcagctaca acatcaaaga atgtgtcctt caatacacca cccaaattat gcctatcgcc
     1201 ttgaataagt tttactccct gaacttgtgg tttagaattt ctgttaagta caaatacttc
     1261 ataacccgca ttcacaaaat attctgccac atatttactt acaaaagttg ttccacctgt
     1321 cactaacatc cttttcataa agttctcctt tataaatccc gattgagatt tcacctgttt
     1381 taagagattt gataactgtt atatcttgtt attaaattct ctgccaaccc ttgaaaacac
     1441 tggatttatc gcagatgagc tttcccttat tctttccctt gtgttatatc gtcccatcag
     1501 tgtttacgaa ttctgataag ggcaaataca agggcaagaa aatcatataa aacagtataa
     1561 cacaaaataa aagtgacatg gtgaactgat tgaaatgctt ctgatttttg tatctgatga
     1621 ttgattgaat gataaggaga taggatacga tgagaacgat atttgcagaa tacaatccac
     1681 acagaaacag tattgatgtt tataccagtg ctggctatat gcttcgcatt gattgttggg
     1741 aagcagaaaa gaatttaaaa accactcccg gatcagactg tgcattaact tcacttgcag
     1801 ttgatgaacc acttgaatat gcaagattat atcttgatgg aaatttgcag atgtgggtga
     1861 acgcggaaga ttcattagag atatagaaat accttttaga acaaagtagt ctttcgcctc
     1921 tacgaatata attgtgaatt agacgcttat atccgaaata aacaattgga acatacccga
     1981 caggagaact ttctttgata agtcacgaaa agaaacagtc tatcagaaaa tgcaatctga
     2041 cacatagacg ggctcgacac atagaataaa caagaggact ttcattcacc ctgttacatt
     2101 ttacagttca cctttctttt tggatatgtc caca
//
LOCUS       DAOMTA010000567         2134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2528_length_2134_cov_2.253006, whole genome shotgun sequence.
ACCESSION   DAOMTA010000567 DAOMTA010000000
VERSION     DAOMTA010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_2134_cov_2.253006"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1037
                     /locus_tag="PLK68_12990"
     CDS             114..1037
                     /locus_tag="PLK68_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM91360.1"
                     /translation="MTTHEVEVIFGITKQTLIYYEKEGLVVCQRDANNYRNYSSKDLD
                     TLKMVLLLRSMEISIDEIKLIMNSELSIRYVLERKQEYIEQLQIELKDIDEKIKAYIQ
                     RHKVEVIFDNSINHEYEGLLINQNEFCFNEVKINCNDIMQIDLSMCSSKGCDGKAYFF
                     LNYYVDLDIHTTSDTYSFQIMNNSQINNLFNYIVSLSVPINDPLGLIRIYRDRNDPVM
                     LNKYLNKHFSSWSQEYNLDNPRENYISIMKESYIYPLKELKDNEHSIDKLVNEQFKAL
                     KTPYTEIFKRASKSINKCFAKLIKRGDCKDD"
     gene            1030..2094
                     /locus_tag="PLK68_12995"
     CDS             1030..2094
                     /locus_tag="PLK68_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91361.1"
                     /translation="MIKMALHLLKLEKKSAVDLCICIASTVTVCLLFMEILKSPEISG
                     NLDFLNIQFLYNALLTFFMIFMCVVLMVYSSNYFIRLKSREIGLTVLSGLSFFKRTIY
                     SLVQVLCIVLFSSMIGIIVFLGISPIVKMVIYEILSVDANIFVLSFDALMQGLAVILA
                     MILVIMLFNSGFIMRTGITELLENHNIISYKKDTRAIKPPDGIYPLIYIFGLICMYTG
                     NNQVGGYILFSFFGAVGAYGVFRHYLPHHYNRNALKYCKTAKDLLIKEDVSLIMQQSK
                     SFILILMVAMIGIVPFICGTTDNSLFNFEMHLAFVITNILLSLTLINRFKIDHIQRRE
                     HFHAIYQLGFARAEIDFELI"
BASE COUNT          758 a          220 c          362 g          794 t
ORIGIN      
        1 attgccaatt ggaaatagtt ttaaaaagca attaattgag ttgtttgata aataatcctt
       61 gactgtattc ttagtatata gtgttaaatg ttaggtagaa gggaggtttt attatgacaa
      121 cacacgaggt tgaagtaatt tttggcataa ctaaacaaac actaatttat tatgaaaaag
      181 aaggcttagt tgtttgtcaa agagatgcaa ataattatcg gaattattca tctaaagatc
      241 ttgatacatt aaaaatggtc ttactattac gttcaatgga aatatccatt gatgagataa
      301 aactaataat gaatagtgaa ctttcaatta gatatgtact tgaaagaaaa caggagtata
      361 ttgaacaatt acaaattgag ttaaaagata ttgatgaaaa aattaaagca tatatacaac
      421 ggcataaagt agaggttatt tttgataatt ctataaatca tgaatacgag ggtctgttga
      481 ttaatcaaaa tgaattttgt tttaatgaag tgaaaattaa ttgtaatgac attatgcaga
      541 ttgatttatc gatgtgtagt tcaaaggggt gtgatggtaa ggcatatttc tttttgaatt
      601 attatgtcga tcttgatatt catactacta gtgatacgta ttcttttcaa ataatgaata
      661 atagtcagat aaataatcta tttaattata tagtttcatt atctgttccg atcaatgatc
      721 cattaggtct tattagaatt tatcgagata gaaatgatcc agtaatgtta aataaatatt
      781 taaataaaca ttttagcagt tggtcccaag aatataattt agataatcct cgtgagaatt
      841 atatttcaat tatgaaagag agttatattt atccattaaa agaattaaag gataatgagc
      901 atagtattga taaacttgta aatgaacaat ttaaagcttt aaaaacacca tatactgaga
      961 tattcaaaag agctagcaaa agtattaata agtgttttgc aaagttaata aaaagaggag
     1021 attgtaaaga tgattaaaat ggcattgcac ttattaaagt tagaaaaaaa gagtgctgtt
     1081 gatttatgta tttgtatagc ttcaacagta actgtttgtt tattatttat ggaaattcta
     1141 aaatcaccag agattagtgg taatttagat tttttaaata ttcaattttt atataatgcg
     1201 ttattaacgt tttttatgat ctttatgtgt gtcgttttaa tggtatattc aagcaattat
     1261 tttattagac ttaaatctag agaaataggg ttaactgtct tatcagggct aagctttttt
     1321 aaacgaacta tatacagttt ggttcaagta ttatgtattg tccttttttc ttcaatgatt
     1381 ggaattatag tttttttggg aataagtcca attgttaaaa tggtgatata tgaaatttta
     1441 agtgttgatg caaatatttt tgtccttagt tttgatgctt tgatgcaggg attagcagta
     1501 attttagcga tgatattggt aattatgtta tttaatagtg gttttattat gcgtactgga
     1561 ataacagaat tacttgaaaa tcataatata attagttata aaaaagatac tcgtgcaata
     1621 aaaccgccgg atgggattta tcctcttatt tatatatttg gcttaatatg tatgtatact
     1681 ggaaataatc aagttggtgg atatatctta ttttcatttt ttggggcagt cggtgcctat
     1741 ggagtgtttc ggcattattt accacatcac tataatagaa atgctttaaa gtactgcaaa
     1801 acggcaaaag acctattaat taaagaagat gtaagtttga ttatgcaaca gtcaaaatct
     1861 tttattttaa tcttaatggt agcaatgatc ggaatcgtgc cattcatttg tgggactact
     1921 gataatagtt tgttcaattt tgagatgcat cttgcatttg ttattaccaa tatattattg
     1981 agtttgacgc ttataaatcg ttttaaaatt gatcatatac aaagaagaga acactttcat
     2041 gccatctatc aacttggctt tgcaagagca gaaattgatt ttgaattaat ataaacagga
     2101 gggcctttgc tctccttagt ttcaaattta attg
//
LOCUS       DAOMTA010000568         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2533_length_2131_cov_1.722543, whole genome shotgun sequence.
ACCESSION   DAOMTA010000568 DAOMTA010000000
VERSION     DAOMTA010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_2131_cov_1.722543"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLK68_13000"
     CDS             <1..585
                     /locus_tag="PLK68_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91362.1"
                     /translation="SGALFSKLFTMLLLRLSDISVDVSFSFSIIPVLLTTALFVTIFA
                     IMIIKGYINILRSSVLDMLSASKQNELREEKIIITLLKVVIGVVLICGGYYAAMMVGD
                     MSSFIYIFYAVVLVVAGIYFLYNGVIPFVIKKLAQNKSYLYQKERSLWVNNLAFRLKK
                     NYRAYAMVTILMICSVTVLATSFAMKQTNCSKNQ"
     gene            641..1465
                     /locus_tag="PLK68_13005"
     CDS             641..1465
                     /locus_tag="PLK68_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91363.1"
                     /translation="MVKSKYLYNQYGVVSYSQLKKAAKEAKIKFDIPKLDDQQVVDLT
                     RLYLLDISDPEINPVIEIAGEKYQIVDETATSYLGLLQENISTYIVSDNTYEKLRNFG
                     QEFYMYNYQIKDPNNYQASIDYLNSLVKDGPVDYVSYLANDPNGGDIAWVRVMYSICI
                     FLFLVFILASGSIIFMKLSNEAFEDRERYNVLKKLGISKRTLSRSIRNEIRFAYYCPF
                     VLMVLSSYFSVHALANVMKTELYTVNIVSAIVILVIFYIIYTISVLMFKKKVLSDH"
     gene            1706..>2131
                     /gene="mscL"
                     /locus_tag="PLK68_13010"
     CDS             1706..>2131
                     /gene="mscL"
                     /locus_tag="PLK68_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRM91364.1"
                     /translation="MKKKSGIIEEFKKFITRGNVMDMAVGVIVGGAFTTIVTSLNNDI
                     ISPILGIFGGVDFSDLKLVMGSGENAPVLKYGSFLTAVINFLIIAMIIFLIVRAMNKI
                     NESIKAKLTDKEEVLVETTKVCPYCREKVAKEATRCPHCT"
BASE COUNT          736 a          231 c          391 g          773 t
ORIGIN      
        1 agtggagcgc ttttttctaa actttttaca atgttgttat tacgcttatc agatataagc
       61 gtggatgttt catttagttt tagtattatt cctgtattac tgacaaccgc tttatttgtc
      121 acgatttttg ctattatgat tattaaaggc tatattaaca ttttaagaag cagtgtttta
      181 gatatgttat cagcatcaaa acaaaatgaa ttacgtgaag aaaaaataat tattacttta
      241 ttaaaagtgg taattggggt agttttgatt tgtggtggct attatgctgc gatgatggtt
      301 ggtgatatgt cttcgtttat ctatatattt tatgcagtag ttttggttgt tgctggaatc
      361 tattttctat ataatggggt aattccgttt gtaattaaaa agttagctca aaacaaaagt
      421 tatctatatc aaaaagaacg ctcattgtgg gtcaataatt tagcatttcg tttaaaaaag
      481 aattatcgtg cttatgcaat ggtaacgatt ttaatgattt gttcggttac cgttttagca
      541 acctcatttg caatgaaaca aacaaattgc tcaaaaaatc agtagtaata atgagctcaa
      601 ctatcataat agtctaaact atattgcctt aaatgcagat atggttaaaa gtaaatatct
      661 atataatcag tatggtgtag tttcatatag ccagttaaaa aaagccgcaa aagaggctaa
      721 aattaaattt gatataccaa aattagatga tcagcaagta gttgatttga cgagattata
      781 tttacttgat atctcagatc ctgaaattaa tccggtgata gaaattgcag gtgaaaaata
      841 tcaaattgtt gatgaaactg caacatcata tttaggatta ttgcaagaaa atattagtac
      901 ctatattgtt agtgataaca catatgaaaa gttaagaaat tttggacaag agttttatat
      961 gtataactat caaataaaag atcctaataa ttatcaagca tcgattgatt atttaaatag
     1021 tttggtaaaa gatggacctg tagattatgt gagttattta gccaatgatc ctaatggtgg
     1081 ggatattgct tgggttcgag taatgtactc tatttgtatt ttcttgtttt tagtattcat
     1141 tttagctagt ggaagtatca tttttatgaa attgagtaat gaggcttttg aggatcgaga
     1201 gagatataat gttctaaaaa aactaggaat cagtaaacga acattgagta ggtcaattag
     1261 aaatgagata cgttttgctt attattgtcc ttttgttttg atggtactgt cctcatattt
     1321 ttctgttcat gcgctagcta atgttatgaa gacagaacta tacactgtaa atattgttag
     1381 tgctatcgtt attttagtaa tcttttatat aatctatact atttcagtat taatgtttaa
     1441 aaagaaagta ttgagtgatc attaaataaa agcaggatga agtttaattt gtcctgttat
     1501 tttttactaa taaagcactt gcagcaagca agaaaagaag atacaagtaa attatatttg
     1561 gctattgaat tgtagaaata aaactagggg tttaattaca taattattat atttaataat
     1621 tatgttgata agctattaag aaaaaacgtt gaaataatag aaatatggta taattaaaca
     1681 tattaacttg ttaggaggtt aaatgatgaa aaaaaaatca ggtattattg aggaatttaa
     1741 gaagtttata actcgaggaa atgttatgga catggctgta ggggtcattg ttggtggtgc
     1801 ttttacaaca atcgtaactt cacttaataa tgatattatt tcaccgattt tagggatttt
     1861 tggcggagtt gatttttctg atttaaaact tgtcatgggg agtggtgaaa atgctccagt
     1921 tttaaagtat ggaagttttt taacggcggt gattaatttc ttaattatag caatgattat
     1981 tttcttaatt gttcgtgcaa tgaataaaat taatgaaagt atcaaagcta aattgactga
     2041 caaggaagaa gtgctggttg aaacaacaaa agtatgccct tattgtagag aaaaagttgc
     2101 gaaagaggca acccgctgtc ctcattgtac t
//
LOCUS       DAOMTA010000569         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2537_length_2127_cov_1.896718, whole genome shotgun sequence.
ACCESSION   DAOMTA010000569 DAOMTA010000000
VERSION     DAOMTA010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2537_length_2127_cov_1.896718"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2127)
                     /locus_tag="PLK68_13015"
     CDS             complement(<1..>2127)
                     /locus_tag="PLK68_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRM91365.1"
                     /translation="ILSKTQEEVEDTTSLSSQKEHEQAKQQKHETPVKKETKLSSHKL
                     LPQFKEDERHNFHITDMDLGVGGSKAKYQMNINAIKLLKQLGAEQRLATSEEQKILSQ
                     YVGWGSLSDAFDESKENWKSEYMELKELLSDSEYKAARESTLTAFYTPPIVIQSIYKK
                     LQDMGLYEGNILEPSCGVGNFIGMQPKGVDYQFYGVELDQISGQIAKQLYQKEIIAVQ
                     GFQEVDMPDNIFDAVIGNVPFGQIPVYDPHYKQNNFMVHDYFFAKALDKVKVGGVVIF
                     ITSHFTMDKKNSSVRKYIAQRADLLGAIRLPDNTFTANAGTKVTSDILILQKREKPYV
                     HEPEWLDLAANDDGLVMNSYFVQHPEMILGQMVEESSPYGKAITCKAIESSSLSEQLK
                     ITFSHITAQISRDSVLYVDEEDRSIPADPNVRNFSYCVVEGSLYYRENSRMYPYETNK
                     TANNRIRGMILIRDCLREVIEAQTNDIDEAQIRSLQKDLHYYYDTFASKYGLINSNAN
                     KRVFKEDSSYGLLCSLEILNEDKTLKRKADIFDKKTIRPHKEITSVSNADEALTVSMA
                     EKGRVDLDYMSHLSNLSKEELIQNLRGVIYQNPNVFDENGNAVYETADEYLSGNVREK
                     LVFAKHAAKTNPELFTINVEALTQVQPEPLKAGDINVRLGSTWVPKKYYEEFMYELLQ
                     TPDFRQPNIKILYVSSTQEWTVTRKSL"
BASE COUNT          631 a          407 c          341 g          748 t
ORIGIN      
        1 taggcttttt cttgtgactg tccattcctg agtgctggaa acatacaata tcttgatatt
       61 aggctgacga aaatcaggtg tctgaagcag ttcatacatg aattcttcat aatatttttt
      121 tggtacccat gtacttccaa gtcgaacatt aatatcaccg gctttcaggg gttcaggttg
      181 tacttgtgtc agtgcttcga cattgatggt aaataattca ggatttgttt tagccgcatg
      241 tttggcaaaa actaattttt cacgaacatt acccgataaa tattcatcgg cagtttcgta
      301 tacagcgttg ccattctcat caaaaacgtt gggattttga taaatgacac ctcgtaaatt
      361 ttgaatcagt tcttctttcg ataaattgga taagtgtgac atataatcta aatcgacacg
      421 tcctttttca gccatactga cggtcaatgc ttcatctgca ttgcttactg aagtaatttc
      481 tttatgaggg cgtatcgtct ttttatcaaa aatatcagct tttcttttta gtgttttatc
      541 ttcatttaag atttcaagtg aacacagcaa gccataagaa ctatcctctt taaagacacg
      601 tttattagcg ttactgttga taagtccata ttttgatgca aaggtgtcat agtaataatg
      661 caaatccttt tgtaaagaac gaatctgtgc ttcatcaata tcgtttgttt gagcttctat
      721 cacttcacgt aaacaatcac gtatcaaaat catacctcgt atcctgttat tggctgtctt
      781 attagtttca tatggataca tacgactatt ttcacgatag tataatgaac cttctacaac
      841 acaataacta aaatttctaa cattaggatc agcaggaatc gaacgatctt cttcatcgac
      901 ataaagaaca gaatctcgac taatctgtgc agtaatatga gaaaatgtta ttttcaactg
      961 ctcagaaagt gatgaagatt ctattgcctt acaagtgatt gcttttccat atggagaact
     1021 ttcttctacc atttgtccta atatcatttc aggatgttga acaaaatagc tgttcattac
     1081 caagccatca tcattggctg ccaagtcaag ccactccggt tcatggacat atggcttttc
     1141 acgtttttgc aagataagaa tatcacttgt tacttttgta ccggcattgg cagtaaaagt
     1201 attgtccggt aagcgaatag cccctagcaa gtcagcacgt tgagcaatat attttctgac
     1261 actgctgttt ttcttatcca ttgtaaaatg agaagtgata aaaataacga caccaccaac
     1321 tttaacttta tccaatgctt tagcaaagaa gtaatcatga accataaaat tgttttgttt
     1381 ataatgtgga tcataaacag gaatttgtcc aaaaggaacg tttccaataa cagcatcaaa
     1441 aatattatcc ggcatatcca cttcctgaaa gccttgtaca gcgattattt ccttttgata
     1501 taactgcttt gctatctgtc cggatatttg atctaattca acaccataaa attgataatc
     1561 aacaccttta ggttgcatac cgataaagtt accgacaccg caagatggtt ctaatatgtt
     1621 cccttcatac aatcccatat cctgtaactt tttataaata gattgaatca caatcggagg
     1681 tgtataaaag gcagtaagag ttgattctct cgctgcctta tattcactgt cactcaataa
     1741 ttctttgagt tccatatatt cacttttcca gttttcttta ctttcgtcaa aagcatcaga
     1801 gagtgaaccc cagccaacat actgtgataa aatcttctgt tcttcagacg tggctaaacg
     1861 ttgttctgca cccaactgtt ttaatagctt gattgcattg atattcattt gatattttgc
     1921 tttagaacca cctacaccca aatccatatc agtaatatga aaattatgtc gttcatcttc
     1981 tttaaactga ggaagcagtt tatgagaaga tagttttgtt tcttttttaa caggtgtttc
     2041 atgcttttgt tgctttgctt gttcatgttc tttttgagaa gataaagatg ttgtatcctc
     2101 cacttcttcc tgtgttttgc ttaatat
//
LOCUS       DAOMTA010000570         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2545_length_2123_cov_2.263540, whole genome shotgun sequence.
ACCESSION   DAOMTA010000570 DAOMTA010000000
VERSION     DAOMTA010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_2123_cov_2.263540"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1162)
                     /gene="metK"
                     /locus_tag="PLK68_13020"
     CDS             complement(20..1162)
                     /gene="metK"
                     /locus_tag="PLK68_13020"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRM91366.1"
                     /translation="MKEKVLFTSESVSKGHPDKVCDQISDAILDACLKEDPNSRVACE
                     VFATTDLVVIGGEITTTAEVNYEQVARDVLKDIGYDDSDKGIDYRTCKVQVVMDLQSP
                     DIALGTNDEVGGAGDQGIMFGYACKETKGYMPLPISIAHHLVRYATEKKDTGEFKSAR
                     PDMKAQVTIDYTESTPKIDTILMSIQHDPDFDETEFKRYIKEEIMDAVVRKYNLNTDY
                     KVLINPTGRFVIGGPHGDTGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYML
                     RYIAKNIVAANLCDKLEIQVSYAIGVKEPTSIFIETYGTEHVDHDIILKAIRDNFDLT
                     PGGIIKTLDLRQPLYLKTAAYGHFGRGDNNLPWEELDKVEILKKYL"
     regulatory      complement(1229..1330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1321..1455)
                     /locus_tag="PLK68_13025"
     CDS             complement(1321..1455)
                     /locus_tag="PLK68_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91367.1"
                     /translation="MKNVRQQKMIASILLDIGLDDDIIEVITSLTKEEIEQINKKDSY
                     "
     gene            complement(1612..1863)
                     /locus_tag="PLK68_13030"
     CDS             complement(1612..1863)
                     /locus_tag="PLK68_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRM91368.1"
                     /translation="MENTSTIEEVEKVINKLRPYLNRDGGDIELIDFKDGIVYVKMLG
                     ACAGCSMLDETLKDGVEQILMEEVPGVLGVQNILEGTEY"
BASE COUNT          684 a          436 c          259 g          744 t
ORIGIN      
        1 ataaatcgag catttttatc tataaatatt tttttagaat ttctacttta tctaactctt
       61 cccatggaag attgttatcc ccacgaccaa agtgtccata agcagctgtt tttagataca
      121 atggttgacg taaatctaac gttttaatga ttcctcctgg agttaaatca aagttatctc
      181 tgattgcttt taggattata tcatggtcaa catgttcagt tccatacgtc tcaataaaaa
      241 ttgaagtagg ttctttaact ccaatagcat aagaaacctg aatttctaat ttatcacaaa
      301 gatttgccgc aacaatattt ttagcaatat aacgcaacat ataggcagct gaacggtcaa
      361 ctttggatgg atcctttcca ctaaaagccc caccaccatg gcgagcagcc ccaccatatg
      421 tatcaacaat aatctttcgc ccagttaagc cagtatcacc atgcggtcct ccaataacaa
      481 agcgaccagt tggattgatc agcactttat aatcagtgtt taaattatat tttcttacaa
      541 ctgcatccat tatttcttct ttaatataac gtttgaattc agtttcatca aaatcaggat
      601 cgtgctgaat cgacattaaa atagtatcaa ttttaggagt actttcggta taatcaatcg
      661 ttacttgagc tttcatatca gggcgggctg acttaaattc tccagtatcc tttttttcag
      721 ttgcataacg aaccaaatga tgagcaattg aaatcggcaa aggcatatac ccttttgttt
      781 ctttacaggc gtaaccaaac atgattcctt gatcccccgc tcctccaact tcatcatttg
      841 ttcctaaagc aatatcagga gactgtaaat ccattactac ctgaaccttg caagtccgat
      901 aatcgattcc tttatcgcta tcatcatatc caatgtcctt taaaacatct cgagctacct
      961 gctcatagtt aacttctgca gtagttgtga tttctccacc aataactacc agatcagtag
     1021 tagcaaaaac ttcacaggcc acacgtgaat taggatcttc ttttaagcac gcatcaagga
     1081 tagcatcact aatttgatca caaactttat caggatgccc ttttgaaact gattcagagg
     1141 taaataatac tttttctttc atttttcttt ctccttctac aaataaaaaa gcttcctaaa
     1201 gagaaagcat acatagttag gaagccttct ctcattgttc aagtataaac ttgctcagtt
     1261 aggcaccttc taatttaata tagggttgcc actacatcat cgatcttcat ctagtagttc
     1321 ttaataagag tcttttttat ttatttgttc tatttcttct ttagttagtg acgttattac
     1381 ctcaataata tcgtcatcga gtccaatatc taataagata ctggcaatca ttttttgttg
     1441 ccttacattc ttcatattat caccttaact tagtttgcat aaattaaaaa ataatattcc
     1501 ttaaaacaac aaatacataa taatatatct aagacattat tgcaagcaat ttattagata
     1561 ctaatatttt agcatggaat gaatcttttt ttgtctcaac tttgttttaa attaatactc
     1621 agttccttct aaaatatttt gcactcctaa aactcctggt acttcttcca ttaagatttg
     1681 ctcaacacca tcctttaaag tttcatctaa cattgaacaa cccgcacagg cacctaacat
     1741 cttcacataa acaatgccat ctttaaaatc tattaattcg atgtcaccac catcacgatt
     1801 taagtacggt cttaatttgt taattacttt ttctacttct tcaattgtac ttgtattttc
     1861 cattgtcctt cactccttta gcacttatct attttaccac aaaaaaatta tttttaaata
     1921 aatatacact ttaattaaga aaattactag gataatatga ttttaactaa aaaccataaa
     1981 aatcacttag atgatctata ttataatcga ctaactgtcg gatgaaaaac actgtaatga
     2041 cggtgttgta aaacaagata cttaataaag accattacta ctaaatatcc tatcttcatc
     2101 actattaatt cttcatcact att
//
LOCUS       DAOMTA010000571         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2548_length_2122_cov_2.142235, whole genome shotgun sequence.
ACCESSION   DAOMTA010000571 DAOMTA010000000
VERSION     DAOMTA010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2548_length_2122_cov_2.142235"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLK68_13035"
     CDS             <1..578
                     /locus_tag="PLK68_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM91369.1"
                     /translation="YQVLDKTCLFIKKVIAEKKDFTGVSINFSVLQFMQDDLENKVLK
                     VIEKHQLPYELIKIEITESMLVTNFDAITNFMTNMINRGIQFLLDDFGTGYSNITYVL
                     TIPFQVVKVDKSLIWQAMKDEKAAILIKKMIEAFNQIGLHILAEGIETKEQMEFMKKC
                     GCDLLQGYYFSRPVSFDEALNVIKTTKITEK"
     gene            complement(643..>1575)
                     /locus_tag="PLK68_13040"
     CDS             complement(643..>1575)
                     /locus_tag="PLK68_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91370.1"
                     /translation="KTLFHCSFNTKQRNTYYNKVEKNYSNSFQLALCLKNIIEQKTIH
                     HVSMSSISSFTILFEKFICLTKLQKQKVLFVCTLDHQNRDLLYRQLLFDIGSYVDIEL
                     FGNVGNILECDINQYDYIISTISMDIDINIPIVLISEFLDNNTMRYFKNLVMETRQIE
                     MNEFLFNKKYYFNCVDIDNINGIYDYLYSVLKKEVAISFDIFKKNLLDKDQVEIIDSC
                     SHSLIFSSHMKLNLKNLLIVFVLKNPIIYNKKKVSIIIFSTLQDDFEGNVRTQGLLKS
                     LRYLCDNEQGFDQFINNPTYVCFIKGIKDISVKK"
     assembly_gap    1576..1585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1586..>2122)
                     /locus_tag="PLK68_13045"
     CDS             complement(<1586..>2122)
                     /locus_tag="PLK68_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM91371.1"
                     /translation="NVGLILEGCEENKRIILSDIIFINYKHSYIMFDFLKSLYKNKEM
                     LDYKIISLLKKYNVSLSDTSLIDFLVYILVMISRAKSKHLLTEIDIPEEFNLSIEMQV
                     AKEIALKIKDTINCQITYDEIKQIAIELFAKEDKGSFYYNKEMSLTIVNESLKAIENE
                     FFIIFKEPLLKDLKEELFT"
BASE COUNT          775 a          299 c          257 g          781 t
ORIGIN      
        1 cttatcaggt acttgataaa acttgtcttt ttataaaaaa agtgattgct gaaaaaaagg
       61 attttaccgg tgtttctatt aatttttcag tattacaatt tatgcaagat gatcttgaaa
      121 ataaagtttt aaaagttatt gaaaaacatc aactgcctta tgaattaata aaaattgaaa
      181 ttaccgaaag tatgctggta actaattttg atgcaatcac aaattttatg acaaatatga
      241 ttaatcgtgg aatccaattc ttactagatg attttggtac tggttattct aatatcacct
      301 atgtccttac tatccctttt caagtcgtta aagtagataa aagcctaatc tggcaagcaa
      361 tgaaagatga gaaagctgca attcttatta aaaaaatgat tgaagcattt aatcaaattg
      421 gtttacatat cttagctgaa ggaatcgaga cgaaggagca aatggaattt atgaaaaaat
      481 gcggctgtga tttattgcaa ggttattatt tttctcgacc agtatcattt gatgaagctt
      541 taaatgtaat caaaacaact aaaattaccg aaaaataata gacatggttg tctataataa
      601 tgacccgatc tcattcatta agtccaacga atgaggagcg ggtcactttt tcacacttat
      661 atccttgatg cctttgataa agcaaacata agtgggatta ttaataaatt gatcaaaccc
      721 ctgctcattg tcacataaat atcttagtga tttcaataat ccttgggttc taacatttcc
      781 ttcaaaatca tcttgtaaag tcgaaaaaat gattattgat acttttttct tattatagat
      841 aatagggttt tttagtacaa atacaattaa tagatttttt aaatttaatt tcatatgact
      901 cgaaaaaata agactatggc tacatgaatc aataatctct acttgatctt tgtctagtaa
      961 gtttttctta aatatatcaa atgaaatagc gacttctttt tttaatacac tatagagata
     1021 atcgtaaatt ccattgatat tatcaatatc aacacaatta aaataatatt tcttattaaa
     1081 taaaaattca ttcatttcga tttgtcttgt ctccattaca agatttttaa agtatctcat
     1141 tgtattgtta tctaaaaact cagatattaa aacaattggg atattgatat caatatccat
     1201 tgaaattgtt gaaatgatat aatcatattg atttatgtca cactctaaaa tattacctac
     1261 attaccaaat agttcaatat caacatacga tcctatatca aacaataact gcctatataa
     1321 taaatctcta ttttgatgat ctagcgtaca tacaaataaa actttctgtt tttggagttt
     1381 tgttaaacaa atgaactttt cgaacaaaat tgtaaaagac gatatactgc tcatcgaaac
     1441 atggtggatt gttttttgct ctattatatt cttgagacat aaagcaagtt gaaatgaatt
     1501 ggagtaattc ttctcaactt tattatagta tgtattacgc tgttttgtat taaaactaca
     1561 atgaaacaat gtcttnnnnn nnnnngtaaa caattcttcc tttaaatctt tcaaaagcgg
     1621 ttctttaaat ataataaaaa actcattttc aattgctttt aatgattcat tgacaatcgt
     1681 taaagacatt tctttattat aataaaatga ccctttatct tctttagcaa acaattcaat
     1741 agcaatttgt ttaatctcat cataggtaat ctggcaattt atagtatctt taatttttaa
     1801 tgcaatctct tttgcaactt gcatttcaat agataagtta aactcttccg gaatatcaat
     1861 ttcagttaat aaatgcttag atttagctct tgaaatcatt accaatatgt aaacgaggaa
     1921 atcaattaaa gatgtatcag ataaagaaac attatatttt tttaacaatg atataatctt
     1981 ataatctaac atctctttat ttttataaag gctctttaaa aaatcaaaca taatatatga
     2041 gtgcttgtaa ttaatgaaaa taatatctga taatataatt cgtttgtttt cttcacatcc
     2101 ttctaaaatc aaccctacat tt
//
LOCUS       DAOMTA010000572         2118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2559_length_2118_cov_2.067378, whole genome shotgun sequence.
ACCESSION   DAOMTA010000572 DAOMTA010000000
VERSION     DAOMTA010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2559_length_2118_cov_2.067378"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..>2118
                     /gene="feoB"
                     /locus_tag="PLK68_13050"
     CDS             5..>2118
                     /gene="feoB"
                     /locus_tag="PLK68_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRM91372.1"
                     /translation="MALAGNPNSGKTTLFNALTGSNQFVGNWPGVTVEKKEGKLKGHK
                     DVKLMDLPGIYSLSPYTLEEVVARNYLIQERPDAIINIVDGTNLERNLYLTTQIMELG
                     IPVIMAINMMDIVAKNNDKIDVKKLSKELGCQVVEISALKGKGIKEAADRAVTLANSK
                     RINAPIHKFDSAVEEKLDQIEAMLPSDIAVEQRRFYAIKLFERDDKISGLMSSVPNVE
                     TIIDQSEKELDDDAESIITNERYQFITSIIDDCYKKHSHDQLSVSDKIDRIVTNRWLA
                     LPIFAVVMFVVYYISVTTVGTWATDWANEGVFGDGWSLFGLAVPSIPSLIEGLLESLG
                     TAAWLNGLILDGIVAGVGAVLGFVPQMLVLFFFLAVLESCGYMARVAFIMDRIFRKFG
                     LSGKSFIPMLIGTGCGVPGIMASRTIENDRDRKMTIITTTFIPCGAKLPIIALIAGAL
                     FGGASWVAPSAYFIGIAAIITSGIILKKTRRFAGEPAPFVMELPAYHMPTITNVLRSM
                     WERGWSFIKKAGTIILLSSILVWFTSYFGFVDGQFTMLEDTQLDHSILASIGNAIAWI
                     FTPLGWGDWKAAVAAITGLVAKENVVGTFGILYGFAEVADDGAEIWGTLAASYTQIAA
                     FSFLIFNLLCAPCFAAMGAIKREMNNGRWTAFAIVYQTVFAYLVAFSVYQIGNLVITG
                     TFTFATVIAILVVIGFVYLLVRP"
BASE COUNT          666 a          274 c          457 g          721 t
ORIGIN      
        1 tacaattgca ttagcgggga atcctaatag tgggaaaaca acgctattta atgctttgac
       61 tggttccaat caatttgttg gaaactggcc aggggtaaca gtagagaaaa aagagggtaa
      121 attaaaaggt cataaagatg ttaaattaat ggatctacct ggtatttatt ctttgtcgcc
      181 atatactttg gaagaggtag tggcacgtaa ctatctaatt caagaaagac cagatgcaat
      241 tatcaatatt gttgatggaa caaacttaga aagaaatctt tatttaacta cacaaattat
      301 ggagttgggg attcctgtta ttatggcaat caatatgatg gatatcgttg ctaaaaataa
      361 tgataagatt gatgtaaaaa aattatcaaa agaattgggg tgccaagttg ttgaaatttc
      421 tgctcttaaa ggaaaaggaa taaaagaagc ggctgatcgt gctgttactt tagctaattc
      481 taaaagaata aatgcaccaa ttcataaatt tgatagtgct gttgaagaaa aattagacca
      541 gattgaagca atgttgccaa gtgatattgc agtggaacaa cgacgttttt atgcgattaa
      601 attatttgaa cgtgatgata aaatttcagg attaatgagt agtgtaccta atgttgaaac
      661 aattattgat caaagtgaaa aagaattaga tgacgatgca gaaagtatta ttactaatga
      721 acgttatcaa tttataactt ctattataga tgattgttat aaaaaacata gtcatgatca
      781 attatcagtt tctgataaaa ttgaccgcat tgttacaaac cgctggttag ccttaccaat
      841 ctttgcagta gtaatgtttg tggtttatta tatttcagta actactgttg gtacttgggc
      901 gactgattgg gcaaatgaag gtgtgtttgg tgatggatgg agtttatttg gactagcagt
      961 tccatcaatt ccaagcttaa ttgaaggatt attagaatcc ttaggaactg cggcctggtt
     1021 aaatggctta attttagatg gtatcgtagc tggggtaggt gcggtattag gtttcgttcc
     1081 acaaatgtta gtgttattct ttttcttagc ggtgcttgaa tcatgtggat acatggctcg
     1141 ggttgcattt attatggata gaatttttag aaaatttgga ttatcaggta aatcattcat
     1201 tccaatgttg attggaactg gatgtggggt accaggaatt atggcatcac gtactattga
     1261 aaatgatcgt gatcgaaaaa tgacgattat tacaactacg tttattcctt gtggtgctaa
     1321 gttaccaatt attgcgttaa ttgcaggtgc attatttggt ggtgcatctt gggtagcacc
     1381 gagtgcatat tttattggaa tagctgcaat tattacttct ggaattattt taaagaaaac
     1441 aagacgtttt gctggtgaac cagcaccatt cgtaatggag ttaccagcgt atcatatgcc
     1501 gacgattact aacgttttaa gaagtatgtg ggaaagagga tggtctttca ttaaaaaagc
     1561 tggaacgatt atcttattat caagtatttt agtttggttt acttcatact ttggatttgt
     1621 cgatggacaa tttacaatgt tagaagatac tcagttagat catagtattt tagcatcaat
     1681 tggtaatgca attgcatgga tatttacacc acttggttgg ggtgattgga aagctgcagt
     1741 agctgcgatt acaggtttag ttgctaaaga aaacgttgtt ggtacattcg gtattttata
     1801 tggatttgca gaggttgctg acgatggagc agaaatctgg ggaaccttag cagcaagcta
     1861 tacacaaatt gcagcatttt cattcttaat ctttaactta ttatgtgcac catgttttgc
     1921 tgctatggga gcaattaaaa gagaaatgaa taatggtcgc tggacagcgt ttgcaattgt
     1981 gtatcaaact gtatttgctt atttagtagc atttagtgtt tatcaaattg gaaatttagt
     2041 tattactgga acatttacat ttgctacagt aattgctata ttagtagtga ttggttttgt
     2101 ttatctatta gttcgacc
//
LOCUS       DAOMTA010000573         2116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2561_length_2116_cov_2.592431, whole genome shotgun sequence.
ACCESSION   DAOMTA010000573 DAOMTA010000000
VERSION     DAOMTA010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_2116_cov_2.592431"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLK68_13055"
     CDS             <1..252
                     /locus_tag="PLK68_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91373.1"
                     /translation="DCESMRRYKFKRNRMIRRVNDVLEQFVEGMILGNLADLEEIEAF
                     NQAWCDEADKMIHEENKNFNQVIDVLKEHWNKEFLAGLI"
     gene            357..>1043
                     /locus_tag="PLK68_13060"
     CDS             357..>1043
                     /locus_tag="PLK68_13060"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM91374.1"
                     /translation="MKVISVINQKGGVGKTQTSINLAVGLSRKGKKVLLIDGDAQGNA
                     TSYFMNDVNKVDLLKLVQMEVTNEDPLVWLEKALGEGEYEKDINDVLLGECDIMEAAY
                     LTGIDNLYIVPSTETRLINTDQLIKTTNKLQHNRLKKALRSIRKDFDYVVIDNAPTFN
                     TITLNTLYASDEVIIPLKIGRFELAGFIQTMKELENLMNDFECFYKIYVLFNMIPRGK
                     RPLYHAFIDKM"
     assembly_gap    1044..1053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1225..1935
                     /locus_tag="PLK68_13065"
     CDS             1225..1935
                     /locus_tag="PLK68_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM91375.1"
                     /translation="MSLTDLQDMKPKMNHLQSLRKVDIEDIRENIHNIFAQRDIEELK
                     ENIKKDGLLKPLEVYQDGDQYILIGGHRRYNALLELYMEDAVEPEINCIVYPKPNSEN
                     DEMLQIITSNAQRNMSKDEMVEVTKILLKVLENEPDRKPYLMDRRTWIGGYLGVCGKT
                     AQEYINIANGKETKKKAKVIDHTFDYAEELLRNKFRTKAKISKNKLTLSFSDVADLNR
                     ILEMMDCLEESSMEVANV"
     gene            1928..>2116
                     /locus_tag="PLK68_13070"
     CDS             1928..>2116
                     /locus_tag="PLK68_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM91376.1"
                     /translation="MFKVILCGKISSDFAVKEVGDSKVCNFTVACKNSFDKYESHVRC
                     SVWGSFVDELVNEDYKDKL"
BASE COUNT          814 a          227 c          439 g          626 t
ORIGIN      
        1 gactgtgaaa gtatgagaag gtacaagttc aaaagaaatc gcatgatcag aagggtaaat
       61 gatgttctag aacagtttgt tgaaggaatg atactaggca atctggctga tttagaagaa
      121 attgaagcat tcaatcaagc ctggtgtgat gaagcagaca aaatgattca tgaagaaaac
      181 aagaacttta atcaggtaat tgatgtttta aaagagcatt ggaataaaga atttcttgcg
      241 ggattgattt agaaggtaaa tttaccttgt agatttgtac ctttgtttag tgatgacgat
      301 ttaaagaaaa tagaagcaga aaggttccag taatgactgg attaaggaaa aacagaatga
      361 aagttataag tgtaattaat caaaaaggtg gcgttggtaa aacgcagaca agcatcaatt
      421 tagcagtagg gctaagcaga aaaggtaaaa aagtactatt gattgatggt gatgcacaag
      481 ggaatgccac tagttatttt atgaatgatg tcaataaagt ggatttattg aaacttgtac
      541 aaatggaagt tactaatgaa gatccattag tttggttaga aaaagcattg ggtgaaggtg
      601 aatatgaaaa agatatcaat gatgttttat taggtgaatg tgacattatg gaagctgcct
      661 atttaacagg aattgataat ttgtacattg taccatcaac tgaaacaaga cttattaata
      721 ccgatcagtt aattaaaaca acaaataaac ttcaacataa tcgtttgaag aaagctttaa
      781 gaagtatcag aaaggatttt gattatgtag tcattgataa tgcaccaaca tttaatacga
      841 ttactttgaa tactttatat gcaagtgatg aagttatcat tccattaaag attggaagat
      901 ttgaattagc tggatttatt cagactatga aagagctaga aaatctaatg aatgatttcg
      961 agtgtttcta taagatttac gtgctgttca atatgattcc tagaggtaaa agacctttat
     1021 atcatgcatt tatagataaa atgnnnnnnn nnnttattta caaataatga catgaaagta
     1081 ctagaaacaa caattggata ccaggaagca gttgcttcta agtctggtat ggcttgtcag
     1141 ttactagtag ataccaaaag taaaattgct gatgattaca gagcattagc tgatgaaatt
     1201 tcaatggaag gataggtgat aaagatgagt ttaacagatc tacaagatat gaagcctaag
     1261 atgaatcatc ttcagtcact tagaaaagtt gatattgaag atatcagaga aaatattcat
     1321 aacatctttg cacaaagaga tattgaagaa ttaaaagaaa atattaaaaa agatggtcta
     1381 ttaaaaccat tggaagttta ccaggatggt gatcaatata tcttgattgg tggtcatcgt
     1441 agatataatg cactattaga actttatatg gaagatgcag tagaaccaga gattaactgt
     1501 atcgtttatc caaaacctaa cagtgaaaat gatgaaatgc ttcagattat tacctcaaat
     1561 gctcaaagaa acatgagtaa agatgagatg gtggaagtaa caaagatatt attgaaagtt
     1621 ttagagaatg aaccggatag aaaaccatat ctgatggata gaagaacatg gattggtggt
     1681 tatctgggtg tatgtggtaa aactgcccag gaatatatca atattgccaa tggtaaggaa
     1741 acgaaaaaga aagctaaagt tattgatcat acttttgatt atgcagaaga gctattaaga
     1801 aataagtttc gtacaaaggc taagattagc aaaaacaaat taacattatc atttagtgat
     1861 gtagcagatt taaatagaat tttggaaatg atggactgtc tagaagagtc atcaatggag
     1921 gtagcaaatg tttaaggtta ttttatgtgg aaagatcagc agtgattttg ctgtaaaaga
     1981 agtaggagat agtaaagtat gtaactttac tgttgcctgt aagaattcgt ttgataaata
     2041 tgaatctcat gtcagatgtt cagtctgggg atcatttgta gatgaattag taaatgaaga
     2101 ttataaagat aagcta
//
LOCUS       DAOMTA010000574         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2568_length_2111_cov_2.638132, whole genome shotgun sequence.
ACCESSION   DAOMTA010000574 DAOMTA010000000
VERSION     DAOMTA010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_2111_cov_2.638132"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /gene="mutL"
                     /locus_tag="PLK68_13075"
     CDS             complement(<1..889)
                     /gene="mutL"
                     /locus_tag="PLK68_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRM91377.1"
                     /translation="MQKIRQLDDVLANKIAAGEVIERPANVVKELVENSIDANSTKID
                     VIIEEGGLNLIQIIDNGEGMVKEDALLCFSRHATSKIKDDQDLFCIQTLGFRGEAIPS
                     IASISNFELKTSTGGTGTTVTYEYGRFVECNESDSKKGTNIKVEKLFQNVPARLKYIK
                     STNAEFANIQTYLERLSLSHPHIAFMLVHNGRTIYKTNGNGNLLEVISNIYGLNVAKA
                     MIPVDFEDDEFHVTGYVSKIDVNRASKNHMVTMVNSRVVKNKVSVDSINNAYRRYLAD
                     KRNPIAIVNIEIDPYLVDVN"
     gene            complement(899..>2111)
                     /gene="mutS"
                     /locus_tag="PLK68_13080"
     CDS             complement(899..>2111)
                     /gene="mutS"
                     /locus_tag="PLK68_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRM91378.1"
                     /translation="QNERDKTGIKGLKVGYNRVFGYYIEVTKGNLSLVKDEFNYTRKQ
                     SLSNAERFVTPELKEMESKLLSAQDKMIKLEYALFTEIRNYIKKDVHAIQDVAKIIAK
                     IDVFQSLAMISSENSYVRPTFNHNKVFKVVDGRHGVIERVMAQGTYVSNDVNIDAANP
                     VMLITGPNMGGKSTYMRTIALIALMGQIGCFVPCSEACIPIFDQIFTRIGASDDLISG
                     QSTFMVEMLEANNALRFATENSLIIFDEIGRGTATFDGMAIAQAMIEYIAAKIKCITL
                     FSTHYHELTFLEEKNLGIKNVHASASIENDDLVFLYRIKPGRSNKSYGVNVAKLAKLP
                     DAVLNRANVLLEALEENNIEHHLSDDTLKEAPPVTVSVVEKYLEGIDPMALSPIDALS
                     TLIELKKLNEK"
BASE COUNT          684 a          424 c          254 g          749 t
ORIGIN      
        1 cattaacatc tactaaataa ggatctattt caatattaac aatcgcaatt ggattacgtt
       61 tatcagctaa atatcgacga tatgcattat taatactatc aacactcact ttatttttaa
      121 ctacccgtga atttaccatt gtcaccatat gattcttact agcacgattc acatcaattt
      181 ttgaaacata acccgtcaca tgaaattcat catcttcaaa atccactgga atcattgctt
      241 tggcaacatt taatccataa atgttcgaga taacttccaa caaattacca tttccattgg
      301 tcttataaat tgttctgcca ttatgaacta acataaaagc aatgtgcgga tgagataaag
      361 atagccgttc caaatatgtt tgaatattag caaactcagc attagtcgat tttatatatt
      421 ttaatcgagc tgggacattt tgaaataatt tttcaacttt gatattagtc ccttttttac
      481 tatcactttc attacactca acaaagcgtc catattcata agtaacagtt gtccctgttc
      541 ctcctgtact cgttttcaat tcaaaattac taattgaagc aatcgaagga attgcttcac
      601 ctctaaaccc taaagtttga atgcaaaaca aatcttgatc atctttgatt ttactagtag
      661 catgacgtga aaaacatagt aaggcatctt ctttgaccat tccctcacca ttatcaatta
      721 tttgaatcaa gtttaatcca ccttcttcaa taatcacatc tatcttcgta ctgtttgcat
      781 caatactatt ttcgactagt tctttaacta catttgccgg tctttcaatc acctcaccag
      841 cagcgatttt attagccaat acatcatcta gctgtcttat tttttgcaaa tatatcacct
      901 acttttcatt taatttcttt aattcaatca atgttgataa agcatcaatt ggtgaaagcg
      961 ccattggatc aataccttct aaatattttt ccactacact tacggttact ggtggagctt
     1021 cttttaaagt atcatcgctt aaatgatgct caatattatt ttcctctaaa gcttctaata
     1081 gtacattagc acgatttaat actgcatctg gtaattttgc taatttagca acattgacac
     1141 cataagattt atttgaccgt cctggtttga tacggtataa aaagacaaga tcatcatttt
     1201 caattgaagc actagcatgg acatttttaa ttccaagatt cttttcttct aagaatgtta
     1261 attcatgata atgagttgaa aataatgtaa tacacttaat ctttgcagcg atatactcga
     1321 tcatagcttg agcaattgcc atcccatcaa aagttgcagt ccctcgacct atctcatcaa
     1381 aaataatcaa tgaattttct gttgcaaagc gtaaagcatt attagcttct aacatttcaa
     1441 ccataaaagt tgactgcccg gaaattaaat catcactagc cccgattcga gtaaaaattt
     1501 gatcaaaaat tggaatacaa gcttcgctac atggaacaaa acaccctatt tgtcccatca
     1561 gtgcaattaa agcaatcgtt cgcatataag tcgatttacc acccatatta ggtcctgtaa
     1621 tcaacatcac tggattagca gcatcaatat ttacatcatt cgaaacatat gttccttgtg
     1681 ccataacccg ttcaattacg ccatgacgac catcaactac tttaaacact ttattatgat
     1741 tgaaagttgg ccgaacataa ctattctcac tagaaatcat tgctaatgat tgaaaaacat
     1801 caattttagc aataatttta gcaacatcct gaatagcatg aacatctttt tttatgtaat
     1861 ttctaatttc tgtaaataat gcatattcta gttttatcat tttatcttgt gcactaagta
     1921 attttgattc catctctttt aattctggtg tcacaaaacg ttcagcatta gataaacttt
     1981 gtttacgagt ataattaaat tcatctttaa ctaatgatag atttccttta gtcacttcaa
     2041 tataatatcc aaagacacgg ttatatccta cttttaatcc ttttattcct gttttatcac
     2101 gctcattttg c
//
LOCUS       DAOMTA010000575         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2569_length_2111_cov_2.104086, whole genome shotgun sequence.
ACCESSION   DAOMTA010000575 DAOMTA010000000
VERSION     DAOMTA010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_2111_cov_2.104086"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="PLK68_13085"
     CDS             <1..179
                     /locus_tag="PLK68_13085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HRM91379.1"
                     /translation="VTNCATRLRVSVKDETLVQPVGVFKKAGAHGLVAKGKAFQVIVG
                     LTVPYVREEFEKLL"
     gene            196..>1189
                     /locus_tag="PLK68_13090"
     CDS             196..>1189
                     /locus_tag="PLK68_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM91380.1"
                     /translation="MKKQSSICIAGGGSTFTPGIVLMLMENQERFPIRQIKLYDNNAE
                     RQNKLAEALGILMKERYPEVAFSWTTDPETAFTDIDFCMAHIRVGLYAMRELDEKIPL
                     KHGVVGQETCGPGGLAYGMRSIGGVLEILDYMEKYSPNAWMLNYSNPAAIVAEATRRL
                     RPNSKIINICDMPIGIERNFATILGLESRKDMIVRYYGLNHFGWWTSITDKEGNDLMP
                     KIKEHCRQFGYAVDTEDFQHRDQSWIDTYTKVKDVYAVDPDTLPNTYLKYYLYPDYVV
                     EHSNKEYTRANEVMDGREKRVFGAAADIVAKQTAKDCGFSTDTHAAYIVDLACAI"
     assembly_gap    1190..1199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1200..1524
                     /locus_tag="PLK68_13095"
     CDS             <1200..1524
                     /locus_tag="PLK68_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM91381.1"
                     /translation="KERMLLIVPNDGAIINFDPTAMVEIPCIVGNEGAEPLKMGEIPQ
                     FQKGLMEQQVSVEKLCVEAWIEGSYQKMWQALTLSKTVPSAKVAKEILDDLIEANKGY
                     WPELK"
     gene            1540..2031
                     /locus_tag="PLK68_13100"
     CDS             1540..2031
                     /locus_tag="PLK68_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91382.1"
                     /translation="MVYGKLQSIFKFFKGMSALDIVKYLFVGIPILILGFLYLFMAQG
                     DIEKMFNEPLIVGQLLLVLAMPFCYLAMRNICQDLKDRKKRDGLLIPLWILIVYQVSS
                     FNMICSFLIIYGTFQEYGKGMFAIKKFTINNSTKAMLVGLIPLGMLYGLVLFVKIRLG
                     ILF"
BASE COUNT          734 a          261 c          426 g          680 t
ORIGIN      
        1 cagttacaaa ctgtgcaact cgcttaagag tatcagttaa agatgaaaca ttagtacaac
       61 cggttggagt ctttaaaaag gctggtgctc atggtttagt tgctaaaggt aaagcgttcc
      121 aggttatcgt tggattaaca gtaccttatg taagagaaga atttgaaaag ttattataaa
      181 aaggggagaa gaagaatgaa aaaacaatca tctatttgta ttgctggcgg tggaagtact
      241 ttcaccccag gaattgttct aatgcttatg gaaaatcaag aacgttttcc aattcgtcaa
      301 attaaattat atgataataa tgcagagcga caaaataaat tagctgaagc attaggaatt
      361 ttaatgaaag aaagatatcc agaagttgcc ttttcttgga caactgatcc agaaacggca
      421 tttacggata ttgatttttg tatggcacat attcgtgttg gtttatacgc aatgcgtgaa
      481 ctagatgaaa aaatcccatt aaaacatggt gttgttggtc aagaaacatg tggacctggt
      541 ggtttagcat atggaatgcg ttcaattggt ggtgttttag aaattttgga ttatatggaa
      601 aagtattctc ctaatgcctg gatgttgaat tattcaaatc cagctgctat cgtagctgaa
      661 gcaactagaa gattacgacc aaattctaaa attattaata tttgtgatat gccaattgga
      721 attgaaagaa attttgctac tattttaggc ttagaatcac gtaaagatat gattgtaaga
      781 tattatggtt taaatcactt tggctggtgg acatcaatta ctgataaaga aggtaatgat
      841 ttaatgccaa aaattaaaga acattgtcgt caatttggtt atgctgttga tactgaagat
      901 tttcaacatc gcgatcaatc ttggattgat acttatacta aagttaaaga tgtatatgcg
      961 gttgatcctg atacattacc aaatacttat ttaaaatatt atttatatcc agattatgta
     1021 gttgaacatt caaataaaga atatacacgt gctaatgaag ttatggatgg acgagaaaaa
     1081 agagtatttg gggcagctgc tgatattgta gcgaaacaaa ctgctaaaga ttgtggattt
     1141 agtacggata cacatgctgc ttatatcgtc gatttagctt gtgcaattgn nnnnnnnnna
     1201 aaagaaagaa tgttgttgat tgttcctaac gatggtgcaa taattaactt tgacccaaca
     1261 gcaatggttg aaattccatg tatcgttggt aatgaaggtg ctgaaccatt aaaaatggga
     1321 gaaattccac aattccaaaa aggtttgatg gaacaacaag tatcagttga aaaattatgt
     1381 gttgaagctt ggatcgaagg aagttatcaa aaaatgtggc aagctttaac attatcaaaa
     1441 acagtaccaa gtgctaaggt agcaaaagaa attttagatg atttaattga agcaaataaa
     1501 ggatattggc cagaattaaa ataataggag gaagtgaaaa tggtgtacgg aaaactacaa
     1561 tcaatattta aattttttaa aggaatgagt gccttggata ttgtaaaata tctatttgtg
     1621 ggtattccta tcttgattct tggattcttg tatttattta tggcccaagg ggatattgaa
     1681 aagatgttta atgaaccttt gattgtggga caattattac tcgttctagc aatgccattt
     1741 tgttatcttg caatgcgcaa tatctgtcaa gatcttaaag atcgaaaaaa acgtgatgga
     1801 ttgttaattc cgttatggat cttaattgtc tatcaagtta gttcatttaa tatgatttgt
     1861 agttttctca tcatttatgg tactttccaa gagtatggta aaggaatgtt tgcaataaaa
     1921 aaattcacta taaataatag tactaaagcg atgttagtgg gattgattcc gcttggtatg
     1981 ttgtacggat tagttttatt tgttaaaatt agattaggaa tactattcta ggaggaatca
     2041 ggaatgttaa atttgtttaa gaaaaaaagt aaatcagtag aagtattagc accactaacc
     2101 gggacgacac t
//
LOCUS       DAOMTA010000576         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2579_length_2105_cov_2.126829, whole genome shotgun sequence.
ACCESSION   DAOMTA010000576 DAOMTA010000000
VERSION     DAOMTA010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_2105_cov_2.126829"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..921
                     /locus_tag="PLK68_13105"
     CDS             91..921
                     /locus_tag="PLK68_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM91383.1"
                     /translation="MYRIEKVLNNNSILASKDNQEVIFLGKGIGFGKKINDIYIPGDG
                     VKKYKMETKEQEKRLPHEIIRNVDPVFIEIASDIIRFAQEQFDHVDTKILLPLADHID
                     FAIKRIQENVNMSNPFTKDIELLFPEEYKVALKGKQLINSITGFEITEDEVGYITLHV
                     HSAISTDHVGESMQAMEIIHESIDKLRKELNIVIDRNSISYIRLMNHIKYLLLRLNTE
                     EKLQMDISDFTQERFPFAYERSKEICIRLSKVMKKEIPQSEIGYLALHLERILSIELK
                     "
     gene            1072..1377
                     /locus_tag="PLK68_13110"
     CDS             1072..1377
                     /locus_tag="PLK68_13110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM91384.1"
                     /translation="MYKILLCCASGLTTSMLVNAMKKDAKEKNIDVMIWAVAESAIDL
                     SWADADCILVAPQNAGDLEKVKGIVNSSIPVASIDGVNFSKMDGQAVLEQAIEMINK"
     gene            1480..>2105
                     /locus_tag="PLK68_13115"
     CDS             1480..>2105
                     /locus_tag="PLK68_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRM91385.1"
                     /translation="MFDVLIKALGFVIVIVIGFLLKQFRILKKEDGYTLATIIMNVTL
                     PCALFSNANGITINGAMIVLILMGIILNVLMVAIGYFVSNGKSAPTRAAYMINCSGYN
                     IGNFVLPFVQAFFPGMGVAYLCMFDVGNALMGLGGTFAIASSVVSSEQKLSVSNVIKK
                     LFSSIPFDVYIVIFFLALFKIKIPAPILSITDFIGAGNGFLAMLMIGL"
BASE COUNT          741 a          218 c          388 g          758 t
ORIGIN      
        1 attatagtaa gtttataatg gtgataaaca tgtgaaacac aaaatgattt tgctgcttaa
       61 ggaaaacgga taagatggga agggataatc atgtatcgaa tagaaaaagt gttaaataat
      121 aatagtattt tagcaagtaa agataatcag gaagttattt ttttaggtaa gggtattggt
      181 tttggtaaaa agataaacga tatttatatt ccaggtgatg gagttaaaaa gtataagatg
      241 gaaacaaaag aacaggaaaa aaggttgcca cacgagatta tcaggaatgt tgatcccgtt
      301 tttattgaaa tcgcatccga tataattaga tttgctcaag aacaatttga tcacgtagat
      361 acaaagattt tattgccgtt agccgatcat attgattttg caattaaaag aattcaagaa
      421 aatgttaata tgtctaatcc gtttaccaaa gatattgaat tactatttcc agaagaatat
      481 aaggtggcat taaaaggtaa acaattaatc aattcaatta ctggtttcga aataactgaa
      541 gatgaagttg gttatataac attacatgtt cattcagcca ttagtactga tcatgttggc
      601 gaatcgatgc aggcaatgga aataattcat gagagtattg ataaattacg aaaagaattg
      661 aatattgtaa ttgatcgtaa ttcaatctct tatattcgac tgatgaatca cattaaatat
      721 ttattattac gtcttaatac tgaagaaaaa ttacaaatgg atattagtga ttttacccaa
      781 gaaagatttc cttttgcata tgaacgttct aaagaaattt gtattcgttt atcaaaggta
      841 atgaaaaaag agattccaca atcagaaatt ggttatttag ctttacattt agaaagaatt
      901 ttatcaattg aactaaaata ggggatattt tactatattt tttcataaaa taaacagtga
      961 atcgttttat gtcaaaattt atttttaaat tctgaaaatt gtagtaattt taatttcatt
     1021 gtgataaaat cgtttcatat gtaaatgata aatgtttatg gaggtatatt tatgtataaa
     1081 attttgctat gctgtgcatc tggtttaact actagtatgc ttgtaaatgc aatgaaaaaa
     1141 gatgcaaaag aaaagaacat agatgtgatg atttgggcag ttgctgaaag cgcaatcgac
     1201 ttatcttggg ctgatgctga ttgtatttta gttgctcctc aaaatgctgg agatcttgaa
     1261 aaggttaaag gtatcgttaa tagttctatt ccggttgctt caattgatgg tgttaacttt
     1321 tctaaaatgg atgggcaagc tgttttagaa caagcaattg aaatgatcaa taaataagtt
     1381 attcgattga ataacttatt tttttatatt ttgaaaaaag aataaaaaaa gtttaaaaag
     1441 tagttatgat tgtcttatca tataaaaagg ggaataaata tgtttgatgt gttaataaag
     1501 gctttaggtt ttgtaattgt aatagttatc ggttttttat taaaacaatt tagaatatta
     1561 aaaaaggaag acggttatac tttggcaaca attattatga atgttacatt gccatgtgca
     1621 cttttttcta atgctaatgg aattacgatc aatggtgcta tgattgtttt aattttaatg
     1681 gggattattt taaacgtttt aatggttgca attgggtatt ttgttagtaa cgggaaatcg
     1741 gcaccaacaa gagcagcgta tatgatcaat tgttcgggct acaatattgg taattttgtt
     1801 ttaccgtttg ttcaagcttt tttcccagga atgggtgtag cttatttatg tatgtttgat
     1861 gttggaaatg cgttaatggg attaggggga acctttgcga ttgcttcaag tgttgttagt
     1921 agtgaacaga agttatccgt aagcaatgta ataaaaaaat tattttcatc aattcctttt
     1981 gatgtatata ttgttatttt ctttttagca ttatttaaaa taaaaatacc agctccaatt
     2041 ttatcaatca ctgattttat tggcgctgga aatggttttt tagcaatgct gatgattgga
     2101 ctgtt
//
LOCUS       DAOMTA010000577         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2588_length_2102_cov_2.034685, whole genome shotgun sequence.
ACCESSION   DAOMTA010000577 DAOMTA010000000
VERSION     DAOMTA010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_2102_cov_2.034685"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..574)
                     /locus_tag="PLK68_13120"
     CDS             complement(401..574)
                     /locus_tag="PLK68_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91386.1"
                     /translation="MTKEIMTIKEFAEVTNIKEYVVRRLVKEKKVVFFMSGKRAYINY
                     PLSIKRLWTNENK"
     gene            complement(788..1114)
                     /locus_tag="PLK68_13125"
     CDS             complement(788..1114)
                     /locus_tag="PLK68_13125"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRM91387.1"
                     /translation="MLVEVEDIVYKILRKKESIIASDYNDTNDELTQCENLLDSIYLK
                     DMRERILLALSQLPLQQSNRIYLVYYLGFSKTEIAHIEGCTEGAIRKSIKRGLLQLKR
                     ILRKNF"
     assembly_gap    1215..1224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1307..1663)
                     /locus_tag="PLK68_13130"
     CDS             complement(1307..1663)
                     /locus_tag="PLK68_13130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM91388.1"
                     /translation="MKIKRGQIYLADLSQGYGSEQGGVRPVLVIQNNKGNKYSPTVII
                     ACITSRYRHKHHLPTHYYIPDTAGLKYRSIVMMEQIKVIDKTRLIKYIGSVTPRFMKI
                     LDKRILISLGIPKKRS"
BASE COUNT          639 a          380 c          231 g          842 t
ORIGIN      
        1 ttataaaata taaatttcct agttgaatca tagaattata ctcctaaata aaatgccccg
       61 actaggggca atttgtacat tcgtctttta tatcccgtac acgaattaac ggataatatt
      121 tgaacgctgg ccttttatat cccgcaagcc agctagcgga taatatttga acattactgt
      181 tcattcaata gtatgtccag atataaacaa atattcatct attattcatc tatatctttc
      241 aactatattt taatgaatat tgatatttaa gtcaatgatt ttcaacttta tacactaata
      301 ttttcattga actatcatgc tctttgaata tctcgttacc tttattttaa ctttttgaaa
      361 gttttaattt ccttatcaca aagatattaa aattcacata ctacttattc tcatttgtcc
      421 ataatctttt tatggaaagt ggataattaa tataggctcg tttaccactc ataaaaaata
      481 caactttctt ttcctttact aatcttctta caacatactc ttttatattt gttacttcag
      541 caaactcttt tattgtcatg atctctttag tcatatataa cacccgcttt catttattcc
      601 aaagtagctg cattgatttt tcatagttaa tataaatacg ttgcatcgtt ttgatatata
      661 caagtttgtt atctttaatc aatcttcgta ctactttaat actgagtccg gttctcaaac
      721 tgaattgctt tatggtaagg atatttttat tttcctcatt cataatttca cactccttta
      781 caaaacttta aaaattcttt ctcaatattc ttttcaactg caaaagacct cttttaatag
      841 atttccttat cgctccctct gtacaacctt caatatgtgc tatctctgtt ttagaaaagc
      901 ctagataata tacaagatat attctgttac tttgctgtaa tggcaactgg cttaatgcta
      961 aaagtatcct ttccctcata tcctttaaat atattgaatc aagcaaattt tcacattgtg
     1021 ttaattcatc gtttgtatca ttataatcac tagctatgat actttctttt ttccttaata
     1081 tcttatatac aatatcttct acttctacta gcacatcttc tttataaata tctggatata
     1141 aatattttaa atttactcgt ttcatctata taggaattaa agtaatattc acttattctg
     1201 ttaatcttgc tgcgnnnnnn nnnnttttta attctctttc ccctcttttt aacatatcct
     1261 tttcaccttc tttcgctatg taaatccagt aactacaata aattcattaa gatctttttt
     1321 taggaatacc taaactaatt aaaatgcgtt tatctaatat cttcatgaat cttggtgtta
     1381 cacttcctat atatttaatt agcctagttt tgtctataac cttgatttgt tccatcataa
     1441 caatagaacg atattttaag cctgccgtat caggtatata gtaatgtgtt ggcaaatggt
     1501 gcttatgtct ataacgactt gttatacaag caataataac cgtaggacta tacttattgc
     1561 ctttgttatt ttgaatcact aatactggtc ttacaccgcc ttgttcagaa ccataacctt
     1621 gacttaaatc agctagatag atctgtcctc ttttaatttt catttgaatt tcctcctatg
     1681 taaaaactag attcagttaa gaatctagtt ttgtcttata agtttttata atatgaatcg
     1741 ttgttttcca tttattctta atcacctgaa aacattaagg aatgctatta gacgaacact
     1801 agcaatgttc tcatgggaat ctcacccttg tgtggtatct cacacaactg catgcagaag
     1861 tatcattatg cctacattac atcattgcca agcacttgct aaatactttt atagatcaat
     1921 aatattcgct cgcatttcac tggtcatggc gtattgtcta aaaggctcat aaagtaggaa
     1981 cgtatctaat agatgttatt taactttcaa agatctatct atgaagtcca actggcttca
     2041 ctataaatgc cttgaaaagt taaaattttg tcctattttt gaaatttttt taattttctt
     2101 at
//
LOCUS       DAOMTA010000578         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2589_length_2102_cov_1.944309, whole genome shotgun sequence.
ACCESSION   DAOMTA010000578 DAOMTA010000000
VERSION     DAOMTA010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2589_length_2102_cov_1.944309"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1138
                     /locus_tag="PLK68_13135"
     CDS             95..1138
                     /locus_tag="PLK68_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91389.1"
                     /translation="MEYYIYMLISLAVMLTAIKLIFCELEGRTAQKKSILSWYTKLNE
                     PFKYTRTKSIIFMCVICYAIASVQAMFTTEWFVEMLGFIAVGVICDGISQYIGFYYNK
                     IRFRKKINEALLMKSEIAKAMNETANALVQQSLPTYSSHEIAARYFNDDTHLATISFD
                     GGEYVAAFEHLPPLTYVVEAQYEKAEEKLADRNVKVTKLTNEGKLPFKDERIDVIVNE
                     LSNYDKYDLYRVVKPGGYIIVNQMGSDNYKELINIFLPFKLHGRWDREASCQTLSDIG
                     LEIIDSVEEHGYVRFDTLASFIQFMKGITKADITQDRFMNFYSHVLKQIKDKKYFELT
                     THRFMVVARKKEL"
     regulatory      2010..>2102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          759 a          222 c          413 g          708 t
ORIGIN      
        1 gatttagaat tcaatgaaaa agggtatttt gtaacagttg atggtgatgc ttattattca
       61 ttgaaaggcg agaaaattta taagaggtaa agaaatggaa tattatattt acatgctaat
      121 ttcgttggca gtaatgttga cagcaatcaa attgattttt tgtgagttag aaggtagaac
      181 ggcacaaaag aaaagtattt taagctggta tactaaatta aatgaaccgt ttaagtatac
      241 tcgaacaaaa agtattatct ttatgtgtgt gatatgttat gctattgcgt cagttcaggc
      301 gatgtttaca actgaatggt ttgttgaaat gttaggattt atcgcagttg gagttatttg
      361 tgatgggatt agccagtata ttggatttta ttacaataaa atccgtttta ggaagaaaat
      421 caatgaggct ttattgatga aatcagaaat tgccaaagcg atgaatgaaa cagctaatgc
      481 gctggtacag caaagtttac caacttattc aagccatgag attgccgccc gttattttaa
      541 tgatgacact catttagcga ctatctcatt tgatggggga gaatatgttg ctgcttttga
      601 acatttacca ccacttacat atgttgtaga agctcaatat gaaaaagctg aagaaaaatt
      661 agctgatcgc aatgttaaag taacaaaatt aacaaatgaa gggaaattac catttaaaga
      721 tgagcgaatc gatgtcattg ttaatgaatt atctaattat gataagtatg atttgtatcg
      781 ggtcgtaaaa ccaggtgggt atattattgt taatcaaatg ggtagtgata attataaaga
      841 attgatcaat attttcttac cttttaagtt acatggtcgt tgggatcgtg aggctagttg
      901 tcagacattg agtgacattg gtttggagat tattgatagt gtggaagaac atgggtatgt
      961 tcgttttgac acgttggcat catttattca gttcatgaaa ggaatcacta aagctgatat
     1021 tactcaagat cgttttatga atttttatag tcatgtgttg aaacagatca aagataagaa
     1081 atattttgaa ttaacgactc atcgttttat ggtagtggca agaaaaaaag agttataaga
     1141 gtatggttaa attgaaaaaa attttcaatt taaccttttt ttatggagaa aaattaatta
     1201 ttacgaaaaa tgataattaa actaccagac aattttttag gagagtttta tctgcgctat
     1261 gaacagaagg tgtttataac attggcaggg ttgtctaaga tcatattgag atatgtaagt
     1321 ctgtgactag aaaattattg ctttttgtaa cgatgttggt tcacgtatca gaaataatat
     1381 gtactgctgt tatgaagtgg atattaagct gtttagaatc tcattgccgg aggagtaatg
     1441 ggtaactgat tatgggtaaa cgcttaaaaa aatgtcgtaa attcatattt aaaattgtat
     1501 taaaggattt tttacagtat tgatagaata aaaagagatg gttttatgat tgttaagaaa
     1561 aaggctttaa atctaaaaag atttaggagc tgataaggta ggtatacttc caatctcgac
     1621 actaaaagaa ttgttaatta gaatattata ttattgaatt taatatacaa cattaaattc
     1681 atgaagtaac atggctaaac agtaattaaa tcaaatatca agcaaggtta agaaaagtat
     1741 cacttatcac accgtatgat taataaacaa aagatgcaag ttatgtagct aatacaatgc
     1801 ggtaaaatta tcaagatgag ttatttagag attatttaaa ttagattgaa gaaaatagtt
     1861 aataaagatg atgaggttat ttttattcat ccaataatgc tggcaggaga taataaaatt
     1921 aaagatctag tattttaaat cagatcatta gtcgaaaaat ggaagtttaa cttccatttt
     1981 tgattcttta ttgtataatt aaagtaattt aataacgcat atttaatggt tgttgtacat
     2041 taataaggaa tcttgttaga agcaagagcg gtcccgccgc cgtaatgagg agttaagacg
     2101 at
//
LOCUS       DAOMTA010000579         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2597_length_2098_cov_2.296133, whole genome shotgun sequence.
ACCESSION   DAOMTA010000579 DAOMTA010000000
VERSION     DAOMTA010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_2098_cov_2.296133"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1183)
                     /locus_tag="PLK68_13140"
     CDS             complement(215..1183)
                     /locus_tag="PLK68_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM91390.1"
                     /translation="MTFDTDKLFRILSLYIENTLIRQAYVEKIRTPVYENIQYEYPLP
                     YHLASIASATIQQHSKIKLSRSELSAFTILFNNAINNQNKKKKKVLLINCMSGSMFTL
                     HRYRIETELAAQLVITKYTQYYRINEEDLSNYDLIISSVPIRKQLSIPVINTSYMITN
                     DDIIRIKSYLSYLFNDEDLVYYFHPYLYSSNVKVKSKKGVASTFYHLLTCLYPNLKDT
                     FKYELNKQHRYTLNTFNNVIGLIKLNKPINANNNIVAITLNEPILFDQQQMQVIILFS
                     CLDNNNIMYNTLFNTLKNVANNELDVKKLLSHLSYPEFLSVIKNNK"
     gene            complement(1282..>2098)
                     /locus_tag="PLK68_13145"
     CDS             complement(1282..>2098)
                     /locus_tag="PLK68_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91391.1"
                     /translation="ELKIIQLLLASPDYISTYDIATSTGIPRRLVRDEISNVRVILSS
                     LNLNLLSKPSKGYFIEGKSSKDLSRLQNIINDNERDENNVFPTLPKDRRNYIAKRLIE
                     EASYIKLETFANELLVSRPTVANEVLLLKKDIHKYGLTLIQKPNYGISIQGSEISKRK
                     VLADWVFENLTQSDMFGDFLDAYFNSPDYQIIEIINQHQIIMSDISLIDFLICFSIAI
                     ARNTINHTIDSPIANFEQFKDRFEYATAKELGKYVQERFKISMNMRFKILLFY"
BASE COUNT          722 a          281 c          337 g          758 t
ORIGIN      
        1 ttgttgatgc ctggttaact ggtgagtatg aaggtgggcg tcatcaagtt agaatcgata
       61 tgttgaaaga aattgaagaa agacaaaaaa gtaaataact gttagtgaat tgtattgtcc
      121 attagtgcct ccctgtacta ataatctctg ccattgataa tataattctt agctaaaata
      181 aagtgtaatg aaaaaacaac aaatgctttt aaagctattt gttgtttttg attacgctga
      241 gaaactctgg atacgataag tgagataata atttttttac atcaagttca ttattagcaa
      301 catttttcag cgtattaaat aaagtattat acataatatt attattgtct aaacatgaaa
      361 agaggataat gacctgcatt tgctgctgat caaacaggat tggttcattt aaagtaattg
      421 cgacgatatt gttattggca ttgattggtt tatttagttt tattagccca atcacattat
      481 tgaaagtgtt caaagtataa cgatgctgtt tatttaactc gtatttgaaa gtatctttta
      541 gatttggata aagacatgtt aataaatgat agaaagttga ggcgacgccc tttttgcttt
      601 tgactttaac attggatgaa tatagatatg gatgaaaata ataaacaagg tcttcatcat
      661 tgaacaaata agataaatag cttttgattc taatgatatc atcattagta atcatataac
      721 tggtattaat tacaggaatc gataattgtt tacgaatcgg tactgaagaa ataattaagt
      781 cataattact taagtcctcc tcattgattc gataatattg agtatattta gtaataacta
      841 attgtgctgc taattcagtt tcaatccgat aacgatgtag tgtaaacata gaaccactca
      901 tacaattgat caataaaact tttttctttt tcttgttttg attgttaata gcattattaa
      961 agagtattgt aaatgccgat aattcactac gtgataactt tatcttggag tgttgttgaa
     1021 tcgttgctga tgcaatagat gctaaatgat atggtagagg atattcatat tgaatatttt
     1081 catataccgg ggtacggatt ttttcaacat atgcttgtct gatcaaagta ttttcaatat
     1141 atagcgatag gattctaaat aatttatcag tatcaaaagt aattaatgtt tctgctttga
     1201 ttctcgctag aatttcatta gcaatggtaa ttacattatc atcatgagaa gcagataagc
     1261 cgcgtgatga ccttttacag attaataaaa tagtaatatt ttgaatctca tattcattga
     1321 aattttaaaa cgttcttgaa catattttcc taattcttta gcagtagcat attcaaaacg
     1381 atccttgaat tgctcgaaat tagcgattgg gctatcaatc gtatgattga tcgtattcct
     1441 tgcaatagcg atagagaaac aaattaaaaa gtcaatcaaa ctaatatcgc tcataatgat
     1501 ttgatgctga ttaatgatct caatgatttg ataatctgga gaattaaaat aggcatccaa
     1561 gaaatcacca aacatatctg attgtgttaa gttttcaaaa acccagtcag ctaagacttt
     1621 tctttttgaa atttcacttc cttgaatact aataccgtaa tttggttttt gaattaaagt
     1681 taaaccatat ttatgaatat ctttttttaa aagaaggacc tcattagcaa cagttggtcg
     1741 acttactaat aattcattag cgaaagtttc taatttaatg tagctggcct cttcaattaa
     1801 tctttttgca atataatttc tacgatcttt tggtaaagtt ggaaaaacat tattttcatc
     1861 acgctcatta tcattaataa tgttttgcaa tcggcttaaa tctttagaag actttccttc
     1921 gataaaataa ccttttgaag gcttggataa taaattaagg tttaatgatg acaaaataac
     1981 tctaacgtta ctaatttcat cacgcactaa acgacgagga attcctgtcg aggtagcgat
     2041 atcgtaggtt gaaatgtagt ctggtgaagc taataataat tgaataattt ttaattcg
//
LOCUS       DAOMTA010000580         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2609_length_2093_cov_2.223258, whole genome shotgun sequence.
ACCESSION   DAOMTA010000580 DAOMTA010000000
VERSION     DAOMTA010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_2093_cov_2.223258"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="nadE"
                     /locus_tag="PLK68_13150"
     CDS             <1..1082
                     /gene="nadE"
                     /locus_tag="PLK68_13150"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRM91392.1"
                     /translation="SELIYGDLDLKKLSSERRKMTTFKSYHNYETIYFDSTNIDLNTT
                     YYYDPHPFVPSNRDLRAKRCKEVFDIQTRGLMQRLKATGIKKVVIGISGGLDSTLALL
                     VCTMAFKKLNYDTKDIIAITMPCFGTTSRTKNNALGLMEELAVTSIEVDITESVRIQF
                     RDIEQDENIHDVTYENVQARTRTEILMNKANQVGGLVIGTGDLSEVALGWSTYNGDHM
                     SMYAVNVSVPKTLVRYLVDYVASLYHGEKLETILKDILDTPVSPELLPQENDQIVQKT
                     EDIVGPYELHDFFIYHMVRFGDEPRKLYRKTKLAFKDKYDKELNFIGVSLANNLNVAV
                     SQMVLKLEVSLSLLVVTGECQVMQT"
     gene            complement(1157..>1357)
                     /locus_tag="PLK68_13155"
     CDS             complement(1157..>1357)
                     /locus_tag="PLK68_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HRM91393.1"
                     /translation="KNQGFDAKNGEWVDMFDKGIIDPTKVTRSALLNAASISALFITT
                     EAGVAEIKSETPEAPMMPNQMY"
     assembly_gap    1358..1367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1368..2093)
                     /locus_tag="PLK68_13160"
                     /pseudo
     CDS             complement(1368..2093)
                     /locus_tag="PLK68_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687962.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
BASE COUNT          689 a          354 c          313 g          727 t
ORIGIN      
        1 acagtgaact gatctatggt gatctcgatt taaagaaatt atcaagtgaa cgtcgaaaaa
       61 tgacaacttt taaatcatat cataattatg aaactattta ttttgattca acaaatatcg
      121 atttaaatac cacatattat tatgatcccc acccatttgt ccctagtaat cgagacttac
      181 gggctaaacg ctgtaaagag gtatttgata tccaaacaag aggtttaatg cagcgcttaa
      241 aagccaccgg aatcaaaaaa gtcgttattg gtatttcggg ggggttagat tctaccttag
      301 ccttattagt atgtacgatg gccttcaaaa aactaaatta tgatacaaag gatattattg
      361 caattacaat gccttgtttt ggaactactt caaggactaa aaacaatgcc ttaggattaa
      421 tggaagaatt agctgtaaca agtattgaag ttgatattac agaatctgtc cgtattcaat
      481 ttagagatat tgaacaagat gaaaatattc atgatgttac ttatgaaaat gttcaagcaa
      541 gaacaagaac agaaattctc atgaataaag ctaatcaagt tggtggtctt gtgattggaa
      601 ctggagattt atcagaagtt gctttaggct ggtcaacata caatggcgat catatgtcaa
      661 tgtatgcagt aaatgtctct gttcctaaaa ccttagttcg ttatttagtt gattacgtag
      721 ctagtttgta tcatggagaa aaattagaaa caatcttaaa agatatacta gatacacctg
      781 tttcaccaga gttattacct caagaaaatg atcagatcgt tcaaaaaact gaagatatcg
      841 taggtccata tgagctgcat gatttcttta tttatcatat ggtccgtttt ggtgatgaac
      901 cacgtaaact atatcgtaaa actaaactag cttttaaaga taaatacgac aaagaactaa
      961 attttattgg cgtttcttta gccaacaatt taaacgtagc tgtatcccag atggtcctaa
     1021 agttggaagt gtctctctct ctcctcgtgg tgactggcga atgccaagtg atgcaaacgt
     1081 aagtaactgg atcgatgaaa tcgaaaaaat ataacaaaaa ggttttgtat ttaaaatcaa
     1141 tacaaaacct tttttattaa tacatttgat ttggcatcat tggagcctct ggtgtttcag
     1201 acttaatctc tgcaacacca gcttcagttg taataaataa agctgagata cttgcagcat
     1261 ttaataatgc actacgagta actttagtcg gatcaataat tcctttatca aacatatcaa
     1321 cccattcacc atttttggca tcaaatcctt gatttttnnn nnnnnnncaa tttgacaaat
     1381 tggtgataat aatgcttcca tcacgatatt aatacctttt tgaacatcaa cattatcatt
     1441 tttaagcact ggttttaatt ctttataagc ttcaactaat gctgctccac caccaattac
     1501 aataccttct gcaactgctg ctttagtagc atttaaagca tcttcaatac gtaatttctt
     1561 ttcttttaat tcactttcag tagtcgctcc aactttaatt gttgcaaccc catttgttaa
     1621 cttaccaagg cgttcttgga attgtttttt atcataatca ctagtactgc tagcaactct
     1681 tgtttttatt tcttcaattc gtgcctttaa ctcaggattt tcttcattac cactgatcat
     1741 agtagtatta tctttagtca caataacttt tttaatagta ccaagttctt ctaattgaag
     1801 ttcttccaac ttcatattta agtctttatt ataaagtttt gcacctgtta atgcagcaat
     1861 atcttgcagc atttcttttt gattatcacc aaaaccagga gccttagttg caacaacatt
     1921 gaaagtacca cgtaatttat ttaaaactaa agttgaaatt acttcatttt cataatcttc
     1981 agcaatcaat aataatggtt tgtttgtctt taaaacttgt tcaagtactg gtaaaatttc
     2041 ttgtaaatta ttaattttat gattagttac taaaacatat ggattttcta att
//
LOCUS       DAOMTA010000581         2092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2611_length_2092_cov_1.796269, whole genome shotgun sequence.
ACCESSION   DAOMTA010000581 DAOMTA010000000
VERSION     DAOMTA010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2611_length_2092_cov_1.796269"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLK68_13165"
     CDS             complement(<1..147)
                     /locus_tag="PLK68_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HRM91394.1"
                     /translation="MTKEQRSRNMSHIKSKDTSIELKVRKYLYHHGFRYRKNDKTIFG
                     KPDLT"
     gene            263..>2092
                     /locus_tag="PLK68_13170"
     CDS             263..>2092
                     /locus_tag="PLK68_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRM91395.1"
                     /translation="MYTLLQSKTLINNTNKTKLLTNNYKQGIKISHELVNQLNSCDSF
                     CFSVAFINKSGLAVLKQSLINLKDRGIRGKIITSTYLGFNHPNVFKELLMFDNIEVRI
                     FEDEEIDFHPKGYIFKHDTNFDIIVGSSNLTQSALSTNQEWNIKIQANKCDDIVTQII
                     GEFDMQWQHSVPLTKSWISEYEKIYVPKPVIPVIKHKNIHPNLMQQEALASLTALRNN
                     HKNKALLISATGTGKTYLSAFDVKNIDPKRILFVVHRENIARDAMNTFKNIIKNHTFG
                     IFTGSDKDINSNYIFSTVQTIHKKEYREMFKPDTFDYIIIDEVHRAGAESYQELMNYF
                     IPSFLLGMSATPERSDNFDIYKMFDYNIAYEIRLQQAMEYNLLCPFHYYGISDFIVDG
                     KTIDDKTKFNDLITTERINHIINKIELYGHSGNRVRGLIFCSRKTEAVELSIEFNKRG
                     YRTCALTGDDSEIKRQKAIDKLESNDGDSLDYIFTVDIFNEGIDIPKVNQVIMLRPTQ
                     SAIVFVQQLGRGLRKSPSKEYVVIIDFIGNYEKNFLIPIALSGNYTYNKDALRRFVSE
                     GSLLMPGASTINFDLVSKKKIYESIDRAKFNDTKIIKDSYLHLK"
BASE COUNT          772 a          297 c          306 g          717 t
ORIGIN      
        1 ggtgaggtct ggttttccaa aaatggtctt gtcatttttt ctatatcgaa aaccgtgatg
       61 atataaatac tttcttactt ttaactctat tgaagtatct ttacttttaa tatgtgacat
      121 atttcttgaa cgttgttctt ttgtcataca atcagccata attatcaccc gtttcttaat
      181 atattttaac attaatacag gtaaatcgct agttattgtc actaaaatgc attatactat
      241 taatatcgac aaggagcaac tcatgtatac attattacaa tctaaaactt taattaataa
      301 tacaaataaa actaaactgc taactaacaa ctataaacaa ggaataaaaa tatcacatga
      361 attagttaat caactaaact cttgtgattc tttttgcttc tctgttgcct ttatcaacaa
      421 atccggttta gcagtactaa aacagtcatt gatcaattta aaagatagag gaattagagg
      481 taaaataata acttcaacat acttaggttt taatcatcct aatgtattta aagaattgtt
      541 gatgtttgat aatattgaag tgcgcatatt cgaggatgaa gaaatcgatt tccatccaaa
      601 aggatatatt tttaagcatg atactaactt tgatatcatt gttggaagct ctaatctaac
      661 ccaatcagca ttatcaacta accaagagtg gaatattaag attcaagcta ataaatgtga
      721 tgacatcgtt acacaaataa taggtgaatt tgatatgcaa tggcaacatt ctgttccttt
      781 aacaaaatca tggattagtg aatatgaaaa aatatatgtt cctaaaccag ttataccagt
      841 tattaaacat aaaaatattc atcctaattt aatgcagcaa gaagccctgg cttcgttaac
      901 agcattacga aataatcata aaaataaagc tttgttgatt tcggcaacag gaactggtaa
      961 aacatatctc agtgcttttg atgttaaaaa tattgatccc aaaagaatat tatttgttgt
     1021 tcatcgcgaa aatattgctc gtgatgctat gaacaccttt aaaaacataa tcaagaatca
     1081 tacttttggt atttttactg gtagtgataa agatattaac agtaattata tcttttcaac
     1141 tgttcaaaca attcataaaa aagaatatcg agaaatgttt aagccagata cttttgatta
     1201 catcattatt gatgaagttc accgtgctgg tgctgagtca tatcaagaat taatgaatta
     1261 ctttatacct agctttctat taggaatgtc agctactcct gaaagatctg ataattttga
     1321 tatatataaa atgtttgatt ataacattgc ttatgaaatt cgtttacaac aggcaatgga
     1381 atataattta ttatgtcctt ttcattatta tggtatatct gattttatag ttgatggaaa
     1441 aaccattgat gataaaacaa aatttaatga cttaataaca actgaacgga taaatcatat
     1501 tattaataaa atagaattat atggtcattc aggtaatcga gttagaggat taatattttg
     1561 cagtcgtaaa actgaagctg ttgaattatc aatagaattt aataaacgtg gttatcgaac
     1621 ctgtgcctta accggtgatg acagtgaaat caaacgtcaa aaagcaatcg ataaattaga
     1681 aagcaatgat ggtgacagtc tagattatat ttttacagta gatatcttta atgaaggaat
     1741 cgatattcct aaggtcaatc aagttattat gttacgccct acccaatcag caattgtctt
     1801 tgtccagcaa ttaggacgag gattacgtaa aagtccatcc aaagaatatg ttgtaattat
     1861 tgattttatt ggtaattatg aaaagaactt cttaatccct attgctttaa gtggaaatta
     1921 tacttacaac aaagatgcct taagaagatt tgtcagtgag ggcagtttat taatgccagg
     1981 agcttccaca atcaattttg atttagtttc taaaaagaaa atctatgaat caattgatcg
     2041 agctaaattt aatgatacta aaatcattaa agacagttat ttacatttaa aa
//
LOCUS       DAOMTA010000582         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2617_length_2088_cov_2.424496, whole genome shotgun sequence.
ACCESSION   DAOMTA010000582 DAOMTA010000000
VERSION     DAOMTA010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2617_length_2088_cov_2.424496"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLK68_13175"
     CDS             complement(<1..621)
                     /locus_tag="PLK68_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91396.1"
                     /translation="MGILLVRLIDVINEYSEDSTFYSIAYTMLLNFDNLQNLSINDVA
                     NLCHVSKSTISKFVRSLNFEDYSDFKAEAYFKENRFNSDYNYVANIQQYIANQDANTY
                     IDKVIQDIEIIKNIDMTVIRKIAQIIYQYPKITAFGTLFSQLGALDLQYKLAYNHKFI
                     MSYVNDVKQDEYLKNNSEQGVVIIYSNSGNYLNKYQLSSFDEKKKYD"
     gene            complement(647..769)
                     /locus_tag="PLK68_13180"
     CDS             complement(647..769)
                     /locus_tag="PLK68_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91397.1"
                     /translation="MDTIKPLKRAISLYKKYVFKKIESHYDNPTDDVIYMKLVF"
     gene            complement(822..929)
                     /locus_tag="PLK68_13185"
     CDS             complement(822..929)
                     /locus_tag="PLK68_13185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91398.1"
                     /translation="MSTDGKAVGCIAYCHHRNQCCEMKRLYVKPDFRNQ"
     gene            complement(952..1089)
                     /locus_tag="PLK68_13190"
     CDS             complement(952..1089)
                     /locus_tag="PLK68_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91399.1"
                     /translation="MLIVDGEKYLAEIKELIIEYTNSLNRDFSFQNIDAELEDLKRRY
                     L"
     gene            1277..1834
                     /locus_tag="PLK68_13195"
     CDS             1277..1834
                     /locus_tag="PLK68_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM91400.1"
                     /translation="MNLPSTSKEEIISICQRLAKEKGLSSINMRSVAIECSVSVGAIY
                     NYFPSKSELLCSTIESIWKDIFHMSSEQFSFTNFIECLTWLFESIQEGSQEYPEFLSK
                     HAMSLAAEDKVVGQKMMKKYFFHLKQNLLIVLKKDQKIRKDAFNQKLDQTLFIEYIFE
                     LFIFSIVNPQQNYQGIIELVERYLY"
     gene            1907..>2088
                     /locus_tag="PLK68_13200"
     CDS             1907..>2088
                     /locus_tag="PLK68_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HRM91401.1"
                     /translation="MKVKKHNLLLIASIVWLIAGFNILKIGIETYVGYTKLLNFFLSI
                     IVFIIFWFAIFYKLTK"
BASE COUNT          750 a          305 c          251 g          782 t
ORIGIN      
        1 gtcgtatttt ttcttttcat caaatgatga taactgatac ttatttaaat agttaccaga
       61 attagaataa atgatcacaa caccttgttc actattattt tttaagtatt cgtcttgttt
      121 aacatcattt acataactca taataaattt atgattatag gccagtttat actgcaaatc
      181 taacgctccc aattgtgaaa aaagtgttcc aaatgccgtg atttttggat attgataaat
      241 tatttgagca atttttctaa tcactgtcat atcaatattt ttgattattt caatatcctg
      301 aataactttg tcaatatagg tatttgcgtc ttgattagca atatattgtt ggatattggc
      361 aacataatta taatcactgt taaaacgatt ttccttaaaa tatgcttctg ccttaaaatc
      421 ggaataatct tcaaaattta atgatcgtac aaacttacta atagttgatt ttgatacatg
      481 acataaattt gcaacatcat taatactaag attttgcaaa ttatcaaaat tcaaaagcat
      541 tgtataagca atactataaa aagtactatc ttcactatat tcatttatta catcaataag
      601 cctaaccaat aatattccca ttaacctatc tcactttctt tgcttattag aatactaatt
      661 tcatataaat tacgtcatcc gttggattat cataatgtga ttcaattttt ttaaaaacat
      721 attttttata taacgaaatc gcccttttta acggtttgat tgtatccaaa accatctcct
      781 tgagcagttt caataattgc ttcaattaat ttttgacctt gtcactgatt acgaaaatct
      841 ggttttacat atagacgttt catttcacag cattgattac gatggtgaca ataagcaata
      901 cagccaacag ctttaccatc agtcgacaac cgctaaaata cgcccatgag actatagata
      961 tcttcttttt aaatcttcta attcagcatc tatgttttga aaagagaaat ctcgattcaa
     1021 tgaatttgta tactcaatga tcaattcttt tatttccgca agatattttt ctccatcaac
     1081 aatcaacatt atttttcacc tcaaatataa tttattttga tactatcatt ctaacataaa
     1141 atagtaatca gccaaactct agattcattt aaaataattg ataaaaaagc aaagtcatta
     1201 tgtttgacta ttataaaaat aaggcgtatt attcaacatg aacatggttc atataaaatt
     1261 ataaggagga aatattttga atttaccatc aacatcaaaa gaagaaataa tttctatctg
     1321 tcaacgttta gctaaagaaa aaggtctatc ttcaattaat atgcgttctg ttgcaattga
     1381 atgtagtgtt tcagtaggcg caatatataa ctattttcca tcaaaatcag aactcttatg
     1441 ttcgactatc gaaagtattt ggaaagatat ttttcacatg tctagtgaac aatttagttt
     1501 tacgaatttc attgaatgtt tgacttggtt atttgaaagt attcaagaag gcagccaaga
     1561 atatcctgaa tttctttcaa aacatgcaat gtctttagct gctgaagata aagtcgttgg
     1621 acaaaaaatg atgaaaaaat attttttcca tttaaaacaa aatttattaa ttgtattaaa
     1681 aaaagatcaa aaaataagaa aagatgcttt taatcaaaaa ttagatcaaa ctttattcat
     1741 tgagtatatt tttgaacttt tcattttttc aatcgttaat ccacaacaaa actaccaagg
     1801 tattattgag ttagttgaac gatatttata ctagcccact tattacgaag tgggaatact
     1861 tataattcaa agtgaacagt gttcagatta attatggagg tttattatga aagtaaaaaa
     1921 gcataattta ttattaatag cttcaatcgt gtggcttatt gctggtttca atattttaaa
     1981 aattggaatt gaaacatatg ttggatatac aaagttatta aatttctttc tctcaatcat
     2041 tgtttttatt attttttggt ttgctatatt ttacaaatta actaaaaa
//
LOCUS       DAOMTA010000583         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2620_length_2087_cov_2.527067, whole genome shotgun sequence.
ACCESSION   DAOMTA010000583 DAOMTA010000000
VERSION     DAOMTA010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_2087_cov_2.527067"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="PLK68_13205"
     CDS             complement(<1..1761)
                     /locus_tag="PLK68_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRM91402.1"
                     /translation="MLTKFDEQAQKAIVVGESIAFDLGHNNVGSEHLLLSLLKISDSK
                     LRELVKKYDVDDKNIYEDIKRLFGTNDDQPFYMEYSDAVKSILEAAMEITNQQNKSKV
                     TLNILTIALLQSEESVAHELLKKYKVDFEEIIYQLNESSELETKLDQIQSLVNLNKKV
                     KQGEHLIVGRQKELEKLCMILSKKEKNNALIIGEAGVGKSALVEKLAYLINQKQVNEG
                     LKKKIIYELSLSSIVAGTKYRGEFEEKLRKIIDKVKEMDNVIIFIDEIHNIIGAGGAE
                     GAIDAANILKPYLARKDLTIIGATTTEEYFQHFEKDQAMNRRFSVITLKENTKEETLE
                     ILQKTKLFYEQFHQIEVDNEVLTYLVDNVDRYIKNRTFPDKAIDIFDLACVKARFKQQ
                     HIITKQIVKDVIEEYTSVKISEDYDYDEIKAKLNHHIIGQSKAIEQIINQLKLTKQSK
                     QPSAVMLFVGNSGVGKSESAKQLSKLLGRKLIRLDMSEYRDSSSVQKIIGAAPGYVGY
                     DKPSLLLGQLQTYPKSIILLDEIDKASQDVINLFLQVFDEGYLEDSHKRKVYFNNTII
                     IMTSNKGTAKNTLGFKKNNHS"
     gene            complement(1855..>2087)
                     /locus_tag="PLK68_13210"
     CDS             complement(1855..>2087)
                     /locus_tag="PLK68_13210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM91403.1"
                     /translation="LDDANYDAIEKTIKEFKQNWQKNITTIDRSSTITLSIGGAYYPK
                     HGKNYQELWSNADKALYLSKNNGRNRISIYQL"
BASE COUNT          640 a          379 c          219 g          849 t
ORIGIN      
        1 actatgatta ttctttttaa atcctaatgt atttttagca gtccctttat ttgacgtcat
       61 aataataatt gtattattaa aatatacttt tcgtttatga ctatcttcta aataaccttc
      121 atcaaatact tgaagaaaaa gatttataac atcttgactt gctttatcaa tttcatctaa
      181 aagaataata cttttaggat aggtttgtaa ttgtcccagt aataaacttg gtttatcata
      241 accaacatat cccggggctg ctccaattat cttttgaaca ctgctgctat cacgatattc
      301 actcatatct aagcgaatca atttacgccc caaaagtttt gaaagctgtt ttgcactttc
      361 actttttcca acaccactat ttccaacaaa taacattact gcacttggtt gttttgactg
      421 ttttgttaat tttaattgat tgattatttg ttcaatcgct ttactttgtc caataatatg
      481 atgattcaat tttgctttaa tttcatcata atcataatcc tcacttatct taactgatgt
      541 atattcttct ataacatctt taacaatttg tttagtaata atatgttgct gcttaaatcg
      601 ggctttaaca caagctaaat caaaaatatc gatggcttta tcagggaaag ttcgattttt
      661 gatataacga tcaacattat caactaaata tgttaaaact tcattatcga cttcaatctg
      721 atgaaattgc tcataaaata atttagtttt ctgtaaaatt tctaatgttt cttctttagt
      781 attttctttt agtgtaataa cactaaaacg tcgattcatt gcttgatcct tttcaaaatg
      841 ctggaaatat tcttctgtcg ttgttgcacc aataatcgtt agatcttttc tcgctaaata
      901 cggttttaaa atattagcag catcaatagc cccttcggcg ccaccagctc caataatatt
      961 atggatctca tcaataaaaa tgatgacatt atccatttcc tttaccttat caataatctt
     1021 tcgtagcttt tcttcaaatt caccacgata ttttgttcct gcaacaatac tagataagct
     1081 caattcataa ataattttct tttttaatcc ttcatttact tgtttttgat taattaaata
     1141 cgctaatttt tctactaaag ctgacttacc tacaccagct tcaccaataa tcaacgcatt
     1201 atttttttct ttcttactta aaatcataca taatttttct aattcttttt gtctaccaac
     1261 aattaaatgt tcaccctgtt ttactttctt attaagatta actagtgatt gaatctgatc
     1321 taatttagtt tctaattcac tactttcatt taattgatag ataatttctt caaaatcaac
     1381 tttatatttc tttaataatt cgtgagctac actttcttca ctctgcaata aagcaattgt
     1441 taggatatta agcgttactt tacttttatt ttgttgatta gtgatttcca ttgctgcttc
     1501 taagatactt tttacagcat cactatactc catataaaaa ggttgatcat catttgtccc
     1561 aaacaacctt tttatatcct catatatatt tttatcatca acatcatatt tcttaactaa
     1621 ttcccgtaat ttggaatcac ttatctttaa cagcgatagc agtaaatgtt cactaccgac
     1681 attattatga cctaaatcaa aagcaatact ttcgcctacc acaattgctt tttgggcttg
     1741 ttcatcaaat tttgttaaca tgttccacct ctaattttat tatatgtttg acctcttaaa
     1801 ttattaacga aacgatttat tttaaacata aaacagttcc ctttcgagaa ctgtttagag
     1861 ttgatatata gatattctgt ttcgaccatt atttttactt aaatatagtg ctttatcggc
     1921 attagaccac aactcttgat aatttttacc atgtttagga taatatgctc ccccgatact
     1981 taaagtaatt gtgctgcttc tatcaattgt tgtaatattc ttctgccaat tttgtttaaa
     2041 ttctttaata gtcttttcaa ttgcatcata attagcatcg tctaaag
//
LOCUS       DAOMTA010000584         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2622_length_2085_cov_2.624631, whole genome shotgun sequence.
ACCESSION   DAOMTA010000584 DAOMTA010000000
VERSION     DAOMTA010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2622_length_2085_cov_2.624631"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLK68_13215"
     CDS             <1..200
                     /locus_tag="PLK68_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRM91404.1"
                     /translation="YQDEYKYFSLYALDDAYIDIDGAEYPLNQYFLQRQDPLCVSNQV
                     SCEIATITNSKPIVLVKSKDA"
     gene            373..>2085
                     /locus_tag="PLK68_13220"
     CDS             373..>2085
                     /locus_tag="PLK68_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRM91405.1"
                     /translation="MARFWDKALKSSLAMLVVFSTLFFMSPNIKVEAKETNYEIYPTP
                     HEITYQDKDYVIRSQVNVVYEDGIDAATKKRMTEVLESKNKQISTSKQKVDGKTNILV
                     GTYKSGGYVDGYVKSNYSVEESLFSKFGAHFVASNNGEIVILGKDTDGAFYGITSLKH
                     IFNQMDGSTIRNFVIKDYADTDIRGFIEGYYGIPWSNEDRMSLMKFGGDFKMTSYVFA
                     PKDDPYHKNLWRELYPEEELAAIKEMVQVGNDSKCRFVWTAHPFMGGFNASKVDEEIA
                     SLLKKFDQLYDAGVRQFGVLGDDVGSLDRSVVIKMMNSVSAWAKEKGDVYDSVFCPAG
                     YNHSWQGDYSELSDYDAGFPEDVKIFWTGEAVCQPVEQKTLDHFKRNRLPEGADERRS
                     PLFWLNWPVNDINGSRLMMGKGSLLHTDINIDDLSGVVTNPMQEAEASKVAIFAVADY
                     AWNVKSFNDDQSWADSFKYIDQEASEELHTLAKHMSNPQPNGHGLVLAESEELQPLIN
                     EFKTKLANGDSIIESGKQLIAEMNVIIDACDGFHANSKNENLKKELLSFTGSLKDLTT
                     AIKHF"
BASE COUNT          720 a          259 c          420 g          686 t
ORIGIN      
        1 tgtatcaaga tgaatataag tatttttcat tatatgcact agatgatgct tatattgata
       61 ttgatggtgc tgagtaccca ttgaatcagt attttttaca acgacaggat ccattatgtg
      121 tatccaatca agtaagttgt gaaattgcaa ctatcacaaa tagtaaacca atcgttctgg
      181 ttaaatcaaa agatgcttga taaatccatt ttttgtaatc gctttcttga caggggataa
      241 ttgtcatgat atactcgtga agtaaatgga ttgttactat tagattaggc aagaccgatg
      301 tacagattat tttctgtata tcggtctttt atttttctaa tccatttgac taaatcttag
      361 ggaggaaaaa agatggcaag attttgggac aaagctttaa aatcttcatt agcaatgttg
      421 gttgtattta gtacactatt ttttatgtca ccaaatataa aagtagaagc taaagaaaca
      481 aattatgaaa tttatccaac accacatgaa attacttatc aagataagga ttatgttatt
      541 cgttcacaag ttaatgtggt atatgaagat ggaattgatg ctgctactaa aaagagaatg
      601 actgaagtat tagagtcaaa aaataaacaa attagcacat ctaagcagaa agtagacgga
      661 aaaacaaata ttttagttgg tacatataaa tctggagggt atgttgatgg ctatgttaaa
      721 tccaactata gcgttgaaga gagtctattt agtaaatttg gggcgcattt tgttgcttca
      781 aataatggtg aaattgttat tttaggaaaa gatactgatg gggcatttta tggaattacc
      841 tcattaaaac atatttttaa tcaaatggat ggatcaacaa tccgtaactt tgtaattaaa
      901 gattatgctg ataccgatat tcgtggtttt atcgaagggt actatgggat tccttggtca
      961 aatgaagata gaatgtcatt aatgaaattt ggtggtgatt tcaaaatgac ttcttatgta
     1021 tttgcaccta aagatgatcc atatcataaa aatttatggc gtgaattata tcctgaagag
     1081 gaactcgcag ccattaagga aatggttcaa gtaggaaatg attctaaatg tagatttgta
     1141 tggacagccc atccatttat gggtggtttc aatgcatcta aagtagatga agaaattgct
     1201 tcattactta aaaagtttga tcaattgtac gatgctgggg tacgccaatt tggtgttctt
     1261 ggtgatgatg ttggtagcct agaccgtagt gttgtaatta aaatgatgaa tagtgtatca
     1321 gcttgggcaa aagaaaaagg agatgtttat gattctgttt tctgtccagc aggatataat
     1381 cattcatggc aaggggatta ttctgaatta agtgattatg atgctggatt ccctgaagat
     1441 gtgaaaattt tctggactgg tgaagcagtg tgtcaaccag ttgaacaaaa aacattagat
     1501 cattttaaaa gaaacagatt accagaaggg gctgacgaaa gaagaagtcc actgttctgg
     1561 ttgaactggc ctgtcaatga tattaacggt tcacgtttaa tgatggggaa aggaagttta
     1621 ttacacactg atattaatat tgatgattta agtggggtag taactaatcc aatgcaagaa
     1681 gctgaagctt ctaaagtggc tatttttgcg gtagctgatt atgcatggaa tgttaaaagc
     1741 tttaatgatg atcaaagttg ggctgattca tttaaatata ttgatcaaga ggcaagtgag
     1801 gaattacata cattagctaa gcatatgtct aatcctcaac ctaatggtca tggcttagtt
     1861 ttagctgaat ctgaagaatt acaaccatta attaatgagt ttaaaactaa gttagctaat
     1921 ggtgattcaa ttattgaaag tggaaaacaa ttaattgcag aaatgaatgt gattattgat
     1981 gcttgtgatg gtttccatgc aaatagtaaa aatgaaaacc tgaaaaaaga attattatct
     2041 tttaccggtt ctttaaaaga tttaacgaca gcaattaaac atttt
//
LOCUS       DAOMTA010000585         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2625_length_2083_cov_2.464004, whole genome shotgun sequence.
ACCESSION   DAOMTA010000585 DAOMTA010000000
VERSION     DAOMTA010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2625_length_2083_cov_2.464004"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLK68_13225"
     CDS             complement(<1..397)
                     /locus_tag="PLK68_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HRM91406.1"
                     /translation="MPTGVIINSLSIVLGGIFGALLGHKLSHRLKTEITLIFGICAMA
                     MGINAIGLMENMPAVIFSIIIGTIVGLSLHFSDWINKGAILMQKPIAKIFKNNGSDLS
                     SEEFQSTLITIIVLFCASGTGIYGSLTSGM"
     gene            607..1647
                     /locus_tag="PLK68_13230"
     CDS             607..1647
                     /locus_tag="PLK68_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM91407.1"
                     /translation="MTKVDIISGFLGAGKTTFIKKLISDVYPGEQIVLIENEFGEIGI
                     DGTFMNNAGIEVTEINSGCICCSLVGDFEASLKEVLETYHPDRIIIEPSGVGKLSDVI
                     KAVSDVHSHELELDNYIAVIDAKKCRLYTKNFGEFFNNQIEYASLIILSRTQDITEFQ
                     LDECLKIIKEHNNQANVITTPWDQLTKEILINAFSNSDNLRDELLEQLDICPICAHHH
                     DHHHDHEHHHDHEHHHDHEHHHDHNCECGHEHHHADEVFTSWGKQTPKKYSKSQLEKI
                     LKELSDSSAYGNILRAKGYVDSIEGDWWYFDLVPGEYEIRIGKPDFTGRICVIGEKLD
                     KIKLDRLFDEVA"
     gene            1647..>2083
                     /locus_tag="PLK68_13235"
     CDS             1647..>2083
                     /locus_tag="PLK68_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM91408.1"
                     /translation="METRVYIFAGFLDSGKTSFINDTLMNPDFYSEEDTLLISFEEGE
                     VAYNDIYLKKSNTSLVTLDFENFTTYDLKHLELEYRPTQIMIEANGMMDLNIFVKETI
                     PENWKVVQVLTTFDTTTFSMYLNNMRSLVYSQVVFSDLVIFNR"
BASE COUNT          750 a          304 c          323 g          706 t
ORIGIN      
        1 tcattcctga agttaaggag ccataaattc ctgttccact agcacaaaat aacacaataa
       61 tagtaattaa agtagattga aactcttccg aacttaaatc actaccatta tttttaaata
      121 ttttggcaat tggcttttgc attaaaattg ctcccttatt gatccaatca ctaaagtgca
      181 gtgataatcc cacaatagtt ccaataatta ttgaaaagat tactgccggc atattttcca
      241 ttaatccaat tgcattgatt cccatagcca tagcacaaat cccaaaaatc aaggttattt
      301 ctgtcttaag tctgtgactt aatttatgac ctaataatgc accaaatatt ccccctaata
      361 caattgataa actattaata atgactcctg ttggcattat ttttccccct ttataatttg
      421 aaaattattt ctctaaaaga ttccattcac tctctttata aagaatatca tttttattag
      481 ataattgcca attaaaatag tttcttgtct tattaagaaa aaaaaggaca taattctata
      541 ggctacaata tttgaaccat aaaatacatg atgttataat ataaagacaa aaaggagatg
      601 aactatatga caaaagtaga tattatatct ggtttccttg gagctggtaa aactacattc
      661 ataaaaaaat taattagtga tgtatatcca ggagaacaaa ttgtcttaat tgaaaatgaa
      721 tttggtgaaa ttggaattga tggaactttt atgaataatg ctggaattga agtaactgaa
      781 attaattcag ggtgtatttg ttgcagtcta gttggtgact ttgaagcttc attaaaagaa
      841 gtattagaaa catatcatcc tgatcgaatt atcatcgaac cttctggtgt tggaaaattg
      901 tcagatgtaa ttaaagctgt tagtgatgta catagtcatg aattagaact tgataattat
      961 attgctgtta tcgatgctaa aaaatgtcga ttatacacaa agaattttgg agagtttttt
     1021 aataatcaaa tcgagtacgc ctcacttatt attttaagcc gaactcaaga tataacagaa
     1081 tttcagctag atgagtgttt aaaaattatt aaagaacata ataatcaagc taatgtcatt
     1141 acaaccccat gggatcagct taccaaagaa atactaatta atgcctttag taatagtgat
     1201 aatcttcgtg atgaattgtt ggaacaatta gatatttgtc ctatatgtgc ccatcatcat
     1261 gatcatcacc acgatcatga acatcatcat gatcacgaac atcatcatga tcacgaacat
     1321 catcatgatc ataattgtga gtgtggtcat gagcaccatc atgctgatga agttttcacc
     1381 agttggggta aacagacacc taaaaaatat tctaaatctc aattagaaaa aatcttaaaa
     1441 gaactaagtg acagttccgc ctatggaaat attttaagag ctaaaggata tgtcgatagt
     1501 attgaaggtg attggtggta ttttgattta gtccctggag aatatgaaat tcgtatagga
     1561 aaacctgact ttacaggtcg aatttgtgtc attggcgaaa aattagataa aataaaatta
     1621 gatagattat tcgatgaggt cgcttaatgg aaacaagagt atatattttc gcaggctttc
     1681 tagatagtgg taaaacctca tttattaatg atactttaat gaatcctgat ttttatagtg
     1741 aagaagacac cttattaatt agttttgaag aaggtgaagt tgcctataat gatatttatt
     1801 taaaaaaatc taatacttca ttggtaacat tagattttga aaattttacc acctatgatc
     1861 taaaacattt agaattagaa tatcgtccta ctcaaatcat gattgaagct aatggaatga
     1921 tggacctgaa tatttttgta aaagaaacga ttcctgaaaa ctggaaagtg gtccaagtat
     1981 taacaacttt tgatactact actttttcaa tgtatttaaa taatatgcgt tctcttgtat
     2041 atagtcaggt tgtctttagt gatcttgtta tttttaatcg cta
//
LOCUS       DAOMTA010000586         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2639_length_2078_cov_2.005437, whole genome shotgun sequence.
ACCESSION   DAOMTA010000586 DAOMTA010000000
VERSION     DAOMTA010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_2078_cov_2.005437"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PLK68_13240"
     CDS             complement(<1..1370)
                     /locus_tag="PLK68_13240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM91409.1"
                     /translation="MEKKLPDNFYWGGSVSSFQTEGARNEGGKGVCIYDVRPTDPEFS
                     DWSVGIDEYHRYDEDIALMKDMGFNFYRFSICWSRIIPDGTLDEPVNEEGIKFYSDLI
                     DKLIAAGIEPMITLVHFDMPYKLVKEHNGFASRYVVDCFERYARVCIERFGNRVKHWM
                     SFNEQNLHGMMLRVSNAEEIPEGVDPAKHLYQVNHNVFIAHCKAVKALRELQPDAQFC
                     GMNAVTNIYPYSNTPKNNLFAWKAYQYMNGFHCDVFAKGKYPDYMIAYLENRGWMPTF
                     EEGDDELLKYTVDYIAFSYYRSNTVTEGEFDYTRPYHEVVGEHTVKNPHCEANEWGWE
                     IDPVGFRWTLNDLATRSDLPVFVLENGIGWREDMTQEEVDTMLAANKTIEDDYRINYH
                     RDHIKEMKNAMFEEGVKCLGYITWGPIDILASMCNMDKRYGFVYVNRTNKDIRDLKRI
                     PKKSYH"
     gene            1522..>2078
                     /locus_tag="PLK68_13245"
     CDS             1522..>2078
                     /locus_tag="PLK68_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91410.1"
                     /translation="MSIMTQLEFELDFSSSEKTIAKYILDNGEDILNLSVKELAKQTY
                     TSPATIVRLCRKLGLNGYGDFKIKYSAELQFDKKNKKRVDVNFPFGNTDSNSQIAYRI
                     ANLHQEAIEDTLNLVDFKNLDKVINLLDQARRIYLFGNGNSLLAGFDFQHKMMRIGKM
                     VEMRAHAGEQGFLSYTCSPDDVAIL"
BASE COUNT          700 a          384 c          283 g          711 t
ORIGIN      
        1 caatgatatg attttttagg aatacgtttt aaatcacgaa tatccttatt agtacggttt
       61 acataaacaa aaccatatcg tttatccata ttacacatac ttgctagaat atcaattggt
      121 ccccaagtaa tgtaacctag acatttaacg ccttcttcaa acattgcatt tttcatttct
      181 tttatgtgat cacgatgata attaatacga taatcatctt cgattgtttt attagctgct
      241 aacattgtat caacttcttc ttgcgtcata tcttcacgcc atccaattcc gttttcaaga
      301 acgaaaactg gtaaatcaga acgtgttgct aaatcattta aagtccatct aaaaccaaca
      361 ggatcaattt cccaacccca ttcattagct tcgcaatgtg gatttttaac tgtatgttct
      421 ccaactactt catgatatgg tcttgtataa tcaaattcac cttcagtaac agtattagag
      481 cgataataac taaaagcaat ataatctaca gtatacttta ataattcatc atcgccttct
      541 tcaaatgttg gcatccatcc acggttttct aaatatgcta tcatataatc tggatatttt
      601 cctttagcaa aaacatcaca gtggaaacca ttcatatatt gataagcttt ccatgcaaat
      661 aaattgttct ttggcgtatt tgaatatgga taaatattag taactgcatt cataccgcag
      721 aattgagcat ctggctgtaa ttctcttaat gctttgacag ctttacaatg agcaataaaa
      781 acattgtgat tgacttgata taaatgttta gctgggtcca caccttcagg aatttcttca
      841 gcgttagata cacgcaacat catcccatgt aaattttgtt cattaaaaga catccagtgt
      901 ttaactcgat taccaaatcg ttcgatacat acacgagcat atctttcaaa acagtcaaca
      961 acatagcgtg atgcaaaacc attatgttct tttactaatt tataaggcat atcaaaatgc
     1021 actaaagtta tcataggttc tataccagca gcaatcaatt tatcaattag atcactgtaa
     1081 aatttaattc cttcttcatt taccggttca tctaaagtcc catcaggaat aattcttgac
     1141 cagcaaattg aaaaacggta aaaattgaat cccatatctt tcattaaagc aatatcttca
     1201 tcataacgat gatattcatc aattcctact gaccagtcag aaaattctgg atcagttggt
     1261 ctaacgtcat aaatacaaac gccttttcca ccttcgttac gagctccttc agtttgaaaa
     1321 cttgatactg agccacccca ataaaaatta tctggtaatt tcttttccat tacaatacct
     1381 ctctttcata taccatttta gttaatctgt aaagataagt aaatgagttt ttctgacctt
     1441 ataatcaagt ttcatatttt atttaataaa taccagtttt atgaaacaat gtttcatgat
     1501 atactaaata gaggtgattt tatgagtatt atgactcaat tagaatttga attagatttc
     1561 tccagttctg aaaaaacaat tgcgaaatac atattagata atggtgaaga tattcttaat
     1621 ttatcagtaa aagaattagc taaacaaact tatacttctc cagcaacaat tgttcgtctt
     1681 tgccgtaaat taggacttaa tggctatggt gactttaaaa tcaaatattc agctgaatta
     1741 cagtttgata aaaaaaacaa aaaacgtgtt gacgtcaact ttccttttgg aaacaccgac
     1801 tcaaatagcc aaattgcata tcggattgcc aatctgcatc aagaagctat tgaagacacc
     1861 ttaaacttag ttgattttaa gaaccttgac aaagttatta atttactcga tcaagcaaga
     1921 cgcatctatc tttttggaaa tggtaattct ttattagctg gttttgattt tcaacacaaa
     1981 atgatgcgaa ttggtaaaat ggtggaaatg cgagctcatg ctggtgagca aggtttctta
     2041 tcatatactt gtagtcctga tgatgtagcc attcttat
//
LOCUS       DAOMTA010000587         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2641_length_2078_cov_1.767672, whole genome shotgun sequence.
ACCESSION   DAOMTA010000587 DAOMTA010000000
VERSION     DAOMTA010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_2078_cov_1.767672"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2078
                     /locus_tag="PLK68_13250"
     CDS             <1..>2078
                     /locus_tag="PLK68_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRM91411.1"
                     /translation="TVYESAYKIYVSDDKDNFGNAVVTRTDNTKKDVTEMISEPVSGR
                     YVKIEVTKQFGYPSVSAREFEVSLTDHKVQDPTANVALKKPGYASSEEDPSVEASNAF
                     DGNASARDSRWGSDIGNGPHWIYTDLGEKYTVKTIKVFWENRKATSYELQVADELSSP
                     MSDSDWTTVKSSTSRPKNKNERIVLDEAVEARYVRLKINSFTADDPDGGVTWNTISIY
                     EMEVYGGELKEDSSEIGDKITIDDVKPGDTHLKVNIPSSEDYDITYNGTDYEQVVDTD
                     YRIYQPIVDTEVSVSFKLVDKKTKDYTFKEIRKTIPGKYSKEEGDNASPVVLPELREW
                     KGFTGKYTIQSSSKVVYKDSSLKKTAQALADDYKEMTGRSLKVVQGTKEDVKAGDIYF
                     ALTTDESKGLQDEGNIIEIQDSISVEAFTTTGAFWATRTILQSLKQGGNEYINKGIAR
                     DYPLYKVRGFILDVGRKTFTMDYLKQLTKEMSWYKMNDLQIHLNDNLIPLEDYTKHDK
                     NVFDAYSAFRLESDVKEGGNGGLNKADLTAKDVWYTKEEFKNYIKESRDYGVNIVPEI
                     DTPAHSLALTKVRPDLRHGTSGRQNDHLNLTTKYDESVEFVQGIFDEYMKGQDPVFDA
                     QTTVHVGADEYSADGTAYRKFCNDMLKYVQDTGRTARIWGSLSSIKGDVEVTSKDVQM
                     NLWNFGWANM"
BASE COUNT          787 a          253 c          392 g          646 t
ORIGIN      
        1 atacagttta tgaaagcgca tataaaattt atgtatctga tgataaagat aactttggta
       61 atgctgttgt aacaagaaca gacaatacaa aaaaagatgt tacagaaatg atttctgaac
      121 ctgtatcagg tcgttatgta aaaattgaag ttactaaaca atttggatat ccttcagtat
      181 ctgcacgtga atttgaagta tctttaacag atcataaagt tcaagatcct actgcaaacg
      241 ttgcattaaa aaaacctgga tatgcaagtt cagaagaaga tcctagtgta gaggcatcta
      301 atgctttcga tggaaatgca tcagctagag attctcgttg gggaagtgat attggaaatg
      361 gtcctcattg gatttataca gatttaggtg aaaaatatac tgtgaaaact ataaaagttt
      421 tctgggaaaa tagaaaggca acaagttatg aacttcaagt tgctgatgaa ttatcatcac
      481 ctatgagtga ttctgactgg actacagtaa aatcatcgac ttcaagacct aaaaataaaa
      541 atgaacgtat tgtattagat gaagctgttg aggcaagata tgtacgttta aaaattaatt
      601 cattcacagc agatgatcct gatggtggag ttacatggaa tacaatttct atttatgaaa
      661 tggaggttta tggtggtgaa ttaaaagaag atagttctga aattggtgat aaaatcacta
      721 ttgatgatgt taaacctgga gatactcatt taaaagtgaa tattccaagt agtgaagatt
      781 atgatattac ttataatgga actgattatg aacaagttgt agatacagat tatagaattt
      841 atcaaccaat cgttgataca gaagtatctg tttcatttaa attggttgat aagaaaacaa
      901 aagactatac ttttaaagaa atcagaaaaa caattcctgg aaaatattct aaagaagaag
      961 gagataatgc ttcaccagta gtattacctg aacttagaga atggaaagga tttactggta
     1021 agtatactat ccaatcatca agtaaagttg tttataaaga tagtagttta aagaaaactg
     1081 cccaagcttt agctgatgat tataaagaaa tgactggtag gtcattaaaa gttgttcaag
     1141 gaactaaaga agatgtaaaa gctggagata tttattttgc attaactact gatgaatcta
     1201 aaggtttaca agatgaaggt aacatcattg aaattcaaga ttctattagt gttgaagcat
     1261 ttacaacaac tggtgctttc tgggcaactc gtactatttt acaatcttta aaacaaggcg
     1321 gtaatgaata tattaataaa ggtatcgcaa gagattatcc tttatataaa gttagaggat
     1381 ttattttaga tgttggtcgt aaaacattta caatggatta cttaaaacaa ttaactaaag
     1441 aaatgtcatg gtataagatg aatgacttac aaattcattt aaatgacaac ttaattcctt
     1501 tagaagatta tactaaacat gataaaaatg tttttgatgc ttattctgca ttccgtcttg
     1561 aatctgatgt taaagaaggt ggaaatggtg gtttaaataa agctgattta actgctaaag
     1621 atgtttggta tacaaaagaa gaatttaaaa actatattaa agaatctaga gattatggtg
     1681 taaatatcgt acctgaaatt gatacaccag cacattcatt agctcttact aaagttagac
     1741 ctgatttaag acatggtact agcggtagac aaaatgatca cttaaactta actacaaaat
     1801 atgatgaatc agtagaattc gtacaaggaa tctttgatga atatatgaaa ggtcaagatc
     1861 cagtatttga tgctcaaaca acagtacatg ttggtgctga tgaatacagt gcagatggta
     1921 ctgcataccg taaattctgt aatgatatgt taaaatatgt tcaagataca ggtagaactg
     1981 ctcggatttg gggaagttta tcatctatca aaggtgatgt tgaagttaca agcaaagatg
     2041 ttcaaatgaa cttatggaac tttggttggg caaatatg
//
LOCUS       DAOMTA010000588         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2651_length_2071_cov_3.324405, whole genome shotgun sequence.
ACCESSION   DAOMTA010000588 DAOMTA010000000
VERSION     DAOMTA010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2651_length_2071_cov_3.324405"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="PLK68_13255"
     CDS             <1..1855
                     /locus_tag="PLK68_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRM91412.1"
                     /translation="RNQKIIKPLNKKSLGKIIDEVFKHSAMSDTSLMLDKLKDQGFYY
                     STIAGTTVSVYDIQVPEAKYEIFEKADEKLEQIKKFYNKGKLTESERYQNVIKLWTEV
                     KDEVQEVVRLEFEADDRNPIFIMSDSGARGSLSNFTQLVGMRGLMSNPKGETIELPIK
                     SSFREGLTASEFFISTHGARKGSTDTALKTADSGYLTRRLVDVAQEVIISEEDCGTDR
                     GFVVTELYNNDDKSVIVPLHDRLVGRYSQKDIFHPETKELIIAGGELITEALADEIVN
                     AGITAVEIRSVLGCNAKGGVCRKCYGRNLATGDVVELGEAVGIMAAQSIGEPGTQLTM
                     RTFHDGGVAGGADITQGLPRIQELFEARNPKAKSIISEIEGEVTNIIDNAGRMEVVIT
                     NDLETRSYLAPYGAKIRVNVGDHVNIGAKITKGSIDPKELLSVADVEAVENYIIKEVQ
                     KVYRIQGIEISDKHIEIIVKQMLRKMKVVEGGETGCLPGTNINVNAFTELNKQVLKDG
                     KHPAVARPVLLGITKASLETESFLSAASFQETTKILTDASIKGKKDHLLGLKENVLIG
                     KLLPAGTGLRGALKSPERLAREAEEAMLASQEDMDDLIDNDDEIEMMEQAG"
BASE COUNT          713 a          296 c          432 g          630 t
ORIGIN      
        1 tagaaatcaa aagattatta aacctttaaa taaaaaatct ttaggaaaga tcattgatga
       61 agtgtttaaa cattcagcaa tgtctgatac aagtttaatg cttgataaat taaaagacca
      121 aggtttctat tactcaacaa ttgctggtac aactgtatct gtatacgata tccaagtgcc
      181 agaagctaaa tatgaaattt ttgaaaaagc tgatgaaaaa ctagagcaaa ttaaaaagtt
      241 ctataataaa ggtaaattaa ctgaatcaga aagatatcaa aatgtaatta aattatggac
      301 tgaggttaaa gacgaagttc aagaagttgt acgtttagag ttcgaagctg atgatcgtaa
      361 cccgatcttc atcatgtctg attctggagc ccgtggatca ttatctaact ttacccaatt
      421 agtaggtatg cgtggattaa tgtcaaatcc aaaaggggaa acaattgagt tgccaatcaa
      481 gtcttcattt agggaaggtt taacagcatc agaattcttt atctctaccc atggtgctcg
      541 taaaggttct accgatacag ctttaaaaac tgccgattct ggatatttaa caagacgttt
      601 agttgatgtt gctcaagaag taattatttc tgaagaagac tgtggaactg accgtggctt
      661 tgtagttaca gaattataca ataatgatga taaatcagtc attgtaccat tacatgaccg
      721 tttggttggt cgttattcac aaaaagatat tttccatcca gaaactaaag aattaattat
      781 tgctggtgga gaattaatta ctgaagcatt agctgatgaa attgttaatg ctggaatcac
      841 tgctgtagag attcgttctg ttttagggtg taatgctaaa ggtggagtat gtcgtaaatg
      901 ttacggacgt aacttagcta caggtgatgt ggttgaatta ggagaagctg taggtattat
      961 ggcggctcaa tcaattgggg aacctggtac ccaattaaca atgcgtacat tccatgatgg
     1021 tggggttgcc ggtggtgccg atattactca agggttacct cgtatccaag agttattcga
     1081 agctcgtaat ccaaaagcaa aatctattat tagtgaaatc gaaggagagg ttactaatat
     1141 tattgataac gctggaagaa tggaagtcgt tattactaat gatttagaaa cgcgtagtta
     1201 tttagcgcca tacggtgcta aaatccgggt gaacgttggt gatcatgtca atattggagc
     1261 taaaatcact aaaggttcaa ttgatccaaa ggaattatta tcagttgctg atgttgaagc
     1321 agttgaaaac tatattatta aagaggttca aaaggtatat cgaattcaag gtatcgaaat
     1381 ttctgataaa catatcgaaa tcatcgttaa acaaatgcta agaaaaatga aagtagttga
     1441 aggtggggaa acaggttgtc tacctggtac aaatattaat gtaaacgcct ttactgaatt
     1501 aaataaacaa gttttaaaag atggtaagca tccagcagta gctcgtccag tattactagg
     1561 gattacaaaa gcatctcttg aaactgaatc attcttatct gcagcatcat tccaagaaac
     1621 tactaagatc ttgaccgatg cttcaatcaa aggtaagaaa gatcatttat taggattgaa
     1681 agaaaacgta ttaattggta agctattgcc agcagggact ggtcttcgtg gagctcttaa
     1741 atcaccggaa agattagcgc gcgaagctga agaagcaatg ttagcgagtc aagaagatat
     1801 ggatgattta attgataatg acgatgaaat agaaatgatg gaacaagcag gttaatccct
     1861 gcttgttttt tatgttaata aatttacttg caatgctagt tactaagtgt taatataaac
     1921 ttgtcactat atagtgacaa agagcggttg tgaatgaatc tcaaatcttg aattagggat
     1981 gtattttgac tctttatggt aaatcacaat tgtgtagtaa aatagggatt ttattttttc
     2041 atggtagtcc tggcagtggg gcacatgcac t
//
LOCUS       DAOMTA010000589         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2658_length_2068_cov_2.474913, whole genome shotgun sequence.
ACCESSION   DAOMTA010000589 DAOMTA010000000
VERSION     DAOMTA010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_2068_cov_2.474913"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1159)
                     /locus_tag="PLK68_13260"
     CDS             complement(251..1159)
                     /locus_tag="PLK68_13260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM91413.1"
                     /translation="MELYSSNKDNLFDYLVDDGHGGKVKKDVSDVGVENLKKAGVDVL
                     VVLGGDGTLTSARDFSRKGVNVIGIPKTMDNDLPATDLTYGFISASSVGTEFIDRLNT
                     TAKSHHRVICCELMGRDAGWIALYSGMAGNASCILIPEIPFTIDNIVKHVAKRDAEGY
                     PYTVIAVAEGAKYADGTKVIGKIVEDSPDPVRYSGLAAKVADDLEQAIANHEVRSVNP
                     GHIIRGGDIQAYDRILSIRFGVKALELIKEGKFGNVVTLKGEEMSYTSLEEVIGDAKF
                     GKQKHVDPNGELVKAAKAIGICFGDE"
     gene            complement(1146..1361)
                     /locus_tag="PLK68_13265"
     CDS             complement(1146..1361)
                     /locus_tag="PLK68_13265"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM91414.1"
                     /translation="MADVKRIALLSGGGDCPGLNAVIRAVTKAAINEYGYEVIGYVYG
                     YRGLYNNDYIELTVDKVENIYKEGGTI"
     gene            1633..>2068
                     /locus_tag="PLK68_13270"
     CDS             1633..>2068
                     /locus_tag="PLK68_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM91415.1"
                     /translation="MRTNEVEKITGLTKHAILYYEKVGIIRPKRTNNGYRNYSHDDLQ
                     TLKLVKFLRNLDISIDDIKAILNNKCSLDDCLKIHKRNIDQTISELEDLQNTISTFKD
                     MHIPLIPALENIKLVPEKKGLGYRKTSKTIAYNRPLTRAMAIR"
BASE COUNT          745 a          398 c          246 g          679 t
ORIGIN      
        1 ccggcctgac cctaaaaaat gatgaataat ttttatttta atggattgat gtttataaat
       61 agaatttaat tcaaaatttt taaaaaaata ttatcaaatg atacttctta tactagacta
      121 atatctaccg caataagaat tattcatttc gactatcttt gattcttttt atgtaataaa
      181 ataatcttaa tagtttttat tcatataata ttatttacat ttttataaaa aataagactg
      241 ttttacagtc ttattcatca ccaaagcaaa ttccaatcgc ttttgcagct tttactaatt
      301 caccattagg atctacatgt ttttgttttc caaatttagc atcaccgatt acttcctcta
      361 aagatgtata agacatttct tcacctttta aagtaacaac attaccaaat ttaccttctt
      421 taatcagctc aagtgctttt acaccaaaac gaattgataa aatacgatca tatgcttgga
      481 tatctcctcc acggatgata tgtcctggat taactgaacg aacttcatgg ttagcaattg
      541 cctgttcaag atcatcagct acctttgcag ctaatcctga ataacgaact ggatcaggac
      601 tatcttcaac gatttttcca atcaccttag tcccatcagc atatttagca ccttctgcta
      661 ctgcaattac agtgtatgga taaccttcag cgtcgcgttt tgcaacgtgc ttaacaatgt
      721 tatcaattgt aaatggaatc tcaggaatta agatacatga ggcatttcct gccattcctg
      781 aatataacgc aatccatcca gcatctcgtc ccatcagctc acaacagata actcgatgat
      841 gtgattttgc cgttgtattt aaccggtcaa taaattcagt tcctactgaa gaagcactga
      901 taaatccata agttaaatca gtcgctggca aatcattatc cattgtttta gggataccaa
      961 ttacattaac gccttttctt gaaaaatcac gagcacttgt taaagtacca tcaccaccta
     1021 aaacaaccag cacatcgaca ccagcttttt ttaagttttc gacaccaaca tcagaaacgt
     1081 ctttttttac ctttccgcca tgaccatcat caacaagata gtcgaataag ttatctttat
     1141 tagaactata tagttccacc ttctttataa atattctcta ccttatcaac tgtcaattct
     1201 atgtaatcat tgttataaag accacgataa ccataaacat agccaataac ttcatacccg
     1261 tattcattaa tagccgcttt ggttactgct ctaataacag catttaaccc ggggcaatct
     1321 ccgcctccag ataataatgc gattcttttt acgtctgcca taaacatccc tccataaact
     1381 ttattatatg attttttata ataaaactca ataaaaatgt taccgtttac ataaaatacg
     1441 caatatttat aaaattcaac aaaatattat aaaaaatatt aacttctgcc ttaaataaac
     1501 aacatctatt tattataaag atgactacat tacttttaaa gattcaataa ataattactc
     1561 aataattaaa ttattattat aaaaacaaaa atatctactt tacgttataa tatctttata
     1621 ggaggggtct ccatgagaac aaatgaagtt gaaaaaatca caggtttaac taaacatgcc
     1681 atactttatt atgaaaaagt gggaatcatc cgacccaaaa gaacaaataa tggttaccgt
     1741 aactacagcc atgacgattt acaaacgtta aaacttgtga aatttttacg taatttagat
     1801 atttcaattg atgatatcaa agcaatctta aataataaat gcagtttaga tgactgttta
     1861 aagattcata agcgcaacat tgatcaaaca atttctgaat tagaagatct tcaaaataca
     1921 atatcaacat tcaaagatat gcatattccg ctaattcctg cattagaaaa tattaaatta
     1981 gttccagaga aaaaagggct tggatatcga aaaacaagta aaacaatagc ttataatcgc
     2041 ccactaacta gagccatggc aatacgac
//
LOCUS       DAOMTA010000590         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2660_length_2068_cov_1.958271, whole genome shotgun sequence.
ACCESSION   DAOMTA010000590 DAOMTA010000000
VERSION     DAOMTA010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_2068_cov_1.958271"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLK68_13275"
     CDS             <1..417
                     /locus_tag="PLK68_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRM91416.1"
                     /translation="KNIYHKKTIAKVTRMSFTGLADSLKVDSIVAPKKIIAGQIIRYV
                     RAKMNKDDDSSVKTLYKIVDGEVEASEFIATPKITFLGMTLNDLNLKNHVLVAAISRE
                     NETIVPKGDTTIELGDHLVIISRGETMKSLNDIIRR"
     gene            423..1862
                     /locus_tag="PLK68_13280"
     CDS             423..1862
                     /locus_tag="PLK68_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRM91417.1"
                     /translation="MNKKMIVFTIGKLLTITGLLMFMPVIVSLIYQETEGVYYAILGI
                     IMLALGILISRKAPRKKNIYAREGFAIVALSWLLISLLGAIPFCLTGEIPSYVDAVFE
                     TVSGFTTTGSSILSNVEALSHTSLFWRSFTHWVGGMGILVFVIAFIPIASGRSLHILR
                     AEVPGPVVGKLVSKVRLTARILYVIYAIMTVIEIILLLFGGMPLFDCILNAFGTAGTG
                     GFGIKNASIAAYDSAYIDGVITVFMILFGINFNLIYFFILGRIKEVLKSEELRWYLII
                     IVAAIALITINILPLYDSILSAFRYSSFQVGSIITTTGFVTADYGQWPVFSQVILLSL
                     MFVGACSGSTGGGIKVSRIVIYFKNARNELHKLLHPHSVRAVEFENQPVEDNVIRTIH
                     AYLVVYITIFALSLLILTFLNLDFKSAFSAIASCLNNIGPGLDVVGPVSNFGSLPDVS
                     KIVLTFDMLAGRLELFPMLLLFSPSLWRK"
BASE COUNT          642 a          276 c          426 g          724 t
ORIGIN      
        1 aaaaatattt atcataaaaa gactattgct aaagttactc ggatgagttt tacaggtctt
       61 gcggatagtc taaaggttga tagtatcgta gctccaaaga aaattattgc tggtcaaata
      121 attcgttatg ttcgagcaaa aatgaataaa gatgatgatt caagtgttaa aactttatat
      181 aagattgtag atggtgaagt ggaagctagt gaatttatcg ctacacctaa aataactttt
      241 ttaggaatga cgttaaatga tttgaattta aagaatcatg tgttagttgc tgctatttcc
      301 cgagaaaatg aaacaattgt acctaagggg gatacgacga ttgaattagg ggatcattta
      361 gtcattattt ctcgtggtga gacaatgaaa agtctaaatg atattataag gagataagca
      421 ggatgaataa aaaaatgatt gtttttacaa tcggtaagtt attaacaatt accggtttat
      481 taatgttcat gccagtaatt gtctcgttga tttatcaaga aacagagggg gtttattatg
      541 cgatacttgg cataataatg ctggctttag gaattctaat ttcaagaaaa gctcctcgta
      601 aaaagaatat ttatgctcgt gagggttttg ctatcgtagc attgagctgg ttattgatat
      661 cattattagg ggccattccg ttttgtttaa ctggtgagat accttcatat gtagatgcgg
      721 tgtttgaaac tgtatcgggt tttacaacaa ccggatcttc gattttatcc aatgtggagg
      781 cattaagcca tacaagttta ttttggcgaa gctttacaca ttgggttgga ggaatgggga
      841 ttcttgtgtt tgtaatcgca tttatcccga ttgcttcagg acgttcatta catattttac
      901 gagctgaggt tcctggaccg gtggttggaa aattggtttc taaagtacga ttaactgcac
      961 gaatcttata tgtgatttat gcaattatga cggttattga aataattctt ttactatttg
     1021 gcggaatgcc gttatttgat tgcattttga atgcttttgg aaccgctggt acaggagggt
     1081 ttggaattaa aaacgcaagt atcgctgctt atgatagtgc ttatattgat ggtgtgataa
     1141 ctgtttttat gatcttattt ggaattaatt ttaacttgat ttatttcttc attttagggc
     1201 gaatcaagga agtattaaaa agcgaagaat tgcgctggta tttgattata attgtcgctg
     1261 caatagcttt aattacgatt aatattttac cattatatga ttcaatctta tctgcattcc
     1321 gttattcaag tttccaggtt gggtcaatca taacgacgac aggatttgta actgctgact
     1381 atgggcaatg gccagtattt tcacaagtaa tattattatc attgatgttt gttggtgctt
     1441 gttcaggctc aactggtggt ggaatcaagg tatcaagaat tgtaatttat tttaaaaatg
     1501 caagaaatga actgcataaa ttattacatc cccatagcgt tcgtgcagtt gaatttgaaa
     1561 atcagccggt tgaagataat gtaatcagaa cgattcatgc atatttagtc gtatatatta
     1621 cgatatttgc tctatcgtta ttgattttaa catttcttaa tcttgatttt aaaagtgctt
     1681 tttcagctat tgcttcttgt ttaaataata tcggtcctgg tttagatgtt gttggaccag
     1741 taagtaattt tggttcgttg ccagatgttt ctaaaatagt tttaactttc gacatgctgg
     1801 caggacgttt agaacttttc ccaatgctgc ttttgttttc accatcactt tggcgaaagt
     1861 agcataaata agaaaaaatc gtgtagaata tagagaggtg ttccaatgaa aaaagaagaa
     1921 atagtcataa aaaatgcatt acgaatgact aaagaggccg atgatcagct ttctatctat
     1981 gccactaaaa atcgtgattg tataaaagta aaacagacga ttagaaatcg tgaagaattt
     2041 gatatccgat ggccgtttga agaagaca
//
LOCUS       DAOMTA010000591         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2670_length_2065_cov_1.878607, whole genome shotgun sequence.
ACCESSION   DAOMTA010000591 DAOMTA010000000
VERSION     DAOMTA010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2670_length_2065_cov_1.878607"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..559
                     /locus_tag="PLK68_13285"
     CDS             32..559
                     /locus_tag="PLK68_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91418.1"
                     /translation="MVKLKRMLGDIDDPTVVVLMALIESQSAETLANWSLNYVEQNLL
                     SIYQDKYPDDARLKHIICETRKYLAGTKTKKEQRMLLKEAKELIKDVEETIVPLAIVR
                     AILIACATKDSPTNALGYTFYAVAAIVYHQAGSCENKEIYDQLAKEEFKKVLKSLQLI
                     AIDNENNPVKVKWYC"
     gene            777..1205
                     /locus_tag="PLK68_13290"
     CDS             777..1205
                     /locus_tag="PLK68_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91419.1"
                     /translation="MKKVIISIFIILFLFLGISYFYYVHNSNARKYINNWNIELSSNL
                     KSLYEKKTEIGFQGDGTIYEIFELEKTSKLPNNLSSNKNTDFEALFKEYLVNLKINTN
                     QYPDFKNDYYWKYIEKNNSDKLLVIINKNKTRLYIIQSLS"
     gene            1280..2020
                     /locus_tag="PLK68_13295"
     CDS             1280..2020
                     /locus_tag="PLK68_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91420.1"
                     /translation="MKNKIKLIRCIGVVICMCLLQTNVYAQSINQEEKTYELLEQQIE
                     SEHIDIIAELDKLTNEYQEILVIETQNKNLTEINKIKDLISGLEKIKKEYMASIQNTT
                     RANQPNTAVAAVIGYFSNKNYKLASELLIHATVNTNKNSTYSPTNGSRVKSHSVFVKI
                     ANGSKTNGSDIFTNTGGTASKDCYYALHSFNYSKPTSSSKLVNISDYYDYASGDYNGM
                     EGIAVNAMYLAQQSGAIVPYNVLISQRL"
BASE COUNT          841 a          239 c          316 g          669 t
ORIGIN      
        1 gaagcaatag atttaagaag gagataattt tatggtaaaa ttaaaaagaa tgctaggcga
       61 tatagatgat cctacggtgg ttgtattaat ggctttaatt gagagtcaaa gtgctgaaac
      121 attagcaaat tggagtttga attacgtgga acaaaattta ttaagtattt atcaagataa
      181 atatcctgat gatgcaaggt taaaacatat tatttgtgaa actagaaaat atttagctgg
      241 tactaaaaca aaaaaagaac aaagaatgct tttaaaagaa gctaaggagc ttattaaaga
      301 tgttgaggag acaattgtac ctttggcaat tgtgcgagca attttaattg catgtgcaac
      361 aaaagattca ccaacaaatg ctctaggtta tactttttat gctgtggcgg caatagtgta
      421 tcatcaagct ggctcatgtg aaaataaaga aatatatgat cagcttgcta aagaagagtt
      481 taaaaaagta ttaaaatcac tgcaactgat agcaatagat aatgaaaata atccagttaa
      541 agttaaatgg tattgttaat taaaaaagag gtataaatga gaaattttta tttaatatat
      601 aggtctattt ttgtgttaaa ttgttttcat caatattaat gttaaatcgt caataaaaaa
      661 ttttttatca ttactttttc ataaaaatta tttgccttta taattctcaa tattacaaag
      721 tacttgttaa taaaaagtac ttttgatata cttaaattag aaaggggaga gaagaaatga
      781 aaaaggttat catatctata tttattattt tatttctatt tctgggaatc tcatattttt
      841 attatgtcca taattcaaat gcgagaaaat acattaataa ttggaacatt gaactatctt
      901 ctaacttgaa atcactatat gagaaaaaaa cagaaatcgg atttcaggga gacggaacta
      961 tttatgaaat ttttgagtta gaaaaaacaa gtaaacttcc aaacaatttg tcttctaaca
     1021 aaaatactga ttttgaagca ttatttaagg aatacttagt taacttgaaa attaatacaa
     1081 atcaatatcc agatttcaaa aatgattatt attggaaata cattgaaaaa aataattccg
     1141 ataaattgtt agtaattatt aacaaaaata agacaaggct atacattatt caatctttat
     1201 cataaccatt ataatttata agaaagaata ttcaaaatct atatattttt aggtaatatt
     1261 tatagaaaga aggaagaata tgaaaaataa aattaagctc atccgatgta ttggtgttgt
     1321 tatttgtatg tgcctgctgc aaactaatgt ttatgcacaa tcaattaatc aagaagaaaa
     1381 aacttatgaa ttattagagc aacaaataga atcagaacat atagatatta ttgcagagtt
     1441 agacaaattg actaatgagt atcaagaaat acttgtaatt gagacacaaa ataaaaatct
     1501 gaccgaaatt aataagatta aagatttaat aagcggttta gagaaaataa aaaaagagta
     1561 catggcttct attcaaaata caactagagc aaatcagcct aataccgctg ttgctgcagt
     1621 aattggttat tttagcaata aaaattataa attagcctct gaattactaa tacatgctac
     1681 tgtaaataca aacaaaaact ccacatatag tccaactaac ggcagtagag ttaagtctca
     1741 cagtgtattt gttaaaatag caaatggttc taagacaaat ggaagtgaca ttttcacaaa
     1801 tactggagga actgcatcaa aggattgtta ttatgcactt catagtttta actatagcaa
     1861 accgacttct agtagtaaat tagttaatat ctcagattat tatgattatg caagtggtga
     1921 ttataatgga atggaaggta ttgcagtaaa tgcgatgtat ctagcacaac agtcgggagc
     1981 catcgttcct tataatgtat taatttccca aagactataa ttttaaatca tctactatat
     2041 aactatctaa atagtaaata gatat
//
LOCUS       DAOMTA010000592         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2675_length_2062_cov_1.958146, whole genome shotgun sequence.
ACCESSION   DAOMTA010000592 DAOMTA010000000
VERSION     DAOMTA010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_2062_cov_1.958146"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..871
                     /locus_tag="PLK68_13300"
     CDS             116..871
                     /locus_tag="PLK68_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91421.1"
                     /translation="MKLEELVNRNYQQLNDNDLYIWNYIIHHRKECERLSIDELALRC
                     SVSRSTILRFSKRLGLKGYAEFKVFLRIDNQKNDKNALTDNIFNHYIETLEQYRDYHY
                     KEIVQSIYEAKNLYVYGTGVIQDNTAQHLKRSFSMVNKLFLDIDVLADFEAYINLFGS
                     NDVFIAISYAGENQRLLDYVYRLKAKGVIVVAIIANNDCTLSHVADYSLHVKTLPVMT
                     NQGRREDLVGNYFILIDFIIANYIEYAKSRGNE"
     gene            871..>1264
                     /locus_tag="PLK68_13305"
     CDS             871..>1264
                     /locus_tag="PLK68_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91422.1"
                     /translation="MTLDKLVEDNYHQLNENDLYIWQYILHHKRECQRISIKDLARNC
                     NVSHTSILRFTKKLGLEGFSELKVHLKWDLAQKPNFKPRIIDDTYHEFIETMERMKNR
                     DLTNIMEMIEKAERVFVYGTGVVQANMAG"
     assembly_gap    1265..1347
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1348..1689
                     /locus_tag="PLK68_13310"
     CDS             <1348..1689
                     /locus_tag="PLK68_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM91423.1"
                     /translation="TNKVFHAVGNGTEIDTILNNVTKNDLFIIISLSGDNETAVTLAR
                     ALRGLHIPRIGIAKVGNTLLSKYCDDMITFRYETFKVGMSDILYGSTAHFFIISDFLF
                     LRYLEYSQEKG"
     gene            1798..1935
                     /locus_tag="PLK68_13315"
     CDS             1798..1935
                     /locus_tag="PLK68_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91424.1"
                     /translation="MVSFTKNYEVPKDAEKGDTIHVVVEVQDNGKHQLKHCQRVIITV
                     K"
BASE COUNT          695 a          209 c          374 g          701 t
ORIGIN      
        1 aaaatcacat caaattgatg tggtttttct ttactgtgat aaattttttt ctaatttagt
       61 tattgatttt gttttactta aaatattgtt ataataatac atgtagaggt atgatatgaa
      121 attagaagaa ctagttaata ggaattatca acaacttaat gataatgatt tatatatatg
      181 gaattatatt attcatcatc gtaaagagtg tgagcgttta tcgattgatg aactagcttt
      241 aagatgtagt gtttcacgaa gtactatttt acgcttttct aagcgtctgg gtttaaaagg
      301 atacgctgaa tttaaagttt ttttacggat cgataatcaa aaaaatgata aaaatgcttt
      361 aacagataat atctttaatc attatataga aactttagaa caatatcggg attatcatta
      421 taaagaaatt gtacaaagta tttatgaagc taaaaatctt tatgtttatg gaactggggt
      481 aattcaagat aatacagcgc aacatttaaa acgttcattt tcaatggtga ataagttatt
      541 tttagacatt gatgtattag cagattttga ggcttatatt aatttgtttg gtagtaatga
      601 tgtttttatt gctatttctt atgctggtga aaatcaacgc ttattagatt atgtttatcg
      661 tttaaaagct aagggagtca ttgttgtggc tattattgct aataatgatt gtactttatc
      721 tcatgtggcc gattatagtc tgcatgtaaa gacattacca gtcatgacaa atcaagggcg
      781 tcgtgaagat ctggtgggga actattttat tttaattgat tttattattg ccaattatat
      841 tgaatatgct aaaagtagag gaaatgaata atgacattag ataaattagt agaagataat
      901 taccatcaat tgaatgaaaa tgacttatat atctggcaat atattttaca tcataaacgg
      961 gaatgtcaaa gaatttcgat taaagatcta gcacgtaatt gcaatgtatc gcatactagt
     1021 attttacgct ttacaaaaaa gctggggctt gaggggttta gtgaattgaa ggtgcatttg
     1081 aaatgggact tggcgcaaaa gccaaatttc aaaccacgaa ttatagacga tacttatcat
     1141 gaatttattg aaacaatgga acgaatgaaa aatcgagatt taactaatat catggaaatg
     1201 attgaaaaag ctgaacgagt ttttgtttat ggaactggag ttgttcaagc aaatatggcc
     1261 ggtgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnaca aacaaggttt ttcatgctgt aggtaacgga
     1381 acggaaatcg atacaatctt aaataatgta actaaaaatg atttgtttat tattatttca
     1441 ttatcgggcg ataacgaaac agcagtaact ttagcacgcg ctttacgagg gttacatatt
     1501 ccaagaattg gaattgctaa ggttggaaat actttattat caaagtattg tgatgatatg
     1561 attactttta gatatgaaac gtttaaagta ggaatgtcag atattcttta tggtagtacc
     1621 gcccacttct ttattatttc agatttttta tttttgagat acttagaata ttcacaagag
     1681 aaaggataga tgtaaagagg atgattaaat gaaactttaa gagttatgaa aatttagtta
     1741 aagataaagg tcctttaatg gctaattatg ctttgatggg agatggtaaa gttattgatg
     1801 gtgagcttta cgaagaatta cgaggtaccg aaggatgctg aaaaaggcga tactattcat
     1861 gtggtcgtag aagttcaaga taatggtaaa catcagttga aacattgtca aagagttatt
     1921 attacagtta aatagtttta tagcagctat attatttata tagctgcttt ttgttgagaa
     1981 ataaatagct tataaatgtt ttaaaggaga gcctgtaaat tattttgtgt aaagtctgtt
     2041 tccaatgata gaatttgatg ag
//
LOCUS       DAOMTA010000593         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2679_length_2060_cov_2.150623, whole genome shotgun sequence.
ACCESSION   DAOMTA010000593 DAOMTA010000000
VERSION     DAOMTA010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2679_length_2060_cov_2.150623"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLK68_13320"
     CDS             complement(<1..1530)
                     /locus_tag="PLK68_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRM91425.1"
                     /translation="MAMNYYQATELYQQTLKKIAHDEFTWKQFLSTSCKNYRLSFDDM
                     VMIYAQRPNAIAVLEMDDWNKKYGLWIKPKSKGIAVFDPHHNGQARLRYYFDISDTRK
                     TNKYRPVPIWSMKPEYEEEVINNLIDTFSLSKSDSMNLADTIIQASEVIVDEGMSDYF
                     SELKYYVNDTFLEELDELNLQLIYTALLKSSISYATLTRCGIIADDYISDDALRLISQ
                     FNSPQALNAIGVPTRDMTQMLIGEIRKSVLECIRSHNRTFELSSQPIYNEHEKEIRHT
                     ERSHHDETRIHSNGRTTDSQHNIKRRTERAAWEIRLDEKTLFERTSPNSVHELSDNSN
                     AQSAFDGNRPDSDATSRLDGGTNDGTARSERSTERIESDGMGTENEQHRGNDSSDTER
                     GTDLRLNSQNEAGNPFGIPAFLSQEVYEELLKKDRFHYHKNKDIVTVFELFEDKEKRI
                     SYVKESFKKMLIEEIILGTRYGYYPDEEKNALKIWKGKYTNPEFEEYVAWEDVTHFIE
                     DM"
     gene            complement(1563..>2060)
                     /locus_tag="PLK68_13325"
     CDS             complement(1563..>2060)
                     /locus_tag="PLK68_13325"
                     /inference="COORDINATES: protein motif:HMM:NF025554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HRM91426.1"
                     /translation="IVLQALDNKPVVITCNYIDERSFYLKNARFSFKEFAELVEKNSC
                     IFYPEHGSSRTYEIYQITSDKGRDYLFMRYSYAEKHFQAKHYEKMYMGMLADKTSLES
                     IFYKHNLDYRPFAQKMRSVSVSDVIVINDYGTRKAYYVDSFGFVEVPKFLAQLQRMKN
                     KEPSR"
BASE COUNT          596 a          392 c          313 g          759 t
ORIGIN      
        1 catatcctca ataaaatgtg taacatcttc ccatgctaca tattcttcaa attcaggatt
       61 tgtatatttt cctttccaaa tctttaatgc attcttttct tcatcaggat aataaccata
      121 gcgtgtccct aagatgattt cctctatcag catcttttta aaggattctt ttacatagga
      181 aatgcgtttt tctttatctt caaaaagttc aaatacagta actatatctt tattcttatg
      241 ataatgaaat ctatcttttt tcagcagttc ctcataaact tcctgtgata aaaaagcagg
      301 tattccaaat ggattacctg cctcattctg tgaatttaat cgtagatcag ttcctctctc
      361 agtatctgat gaatcgtttc ctcgatgttg ttcattttct gtacccattc catctgattc
      421 gattctttca gtgcttcgtt cactccttgc tgttccatca tttgtgccac catcaagtcg
      481 tgacgttgca tcgctatctg gtcgatttcc atcaaatgct gattgagcgt ttgagttgtc
      541 agataattcg tgaactgaat tgggcgatgt tctttcaaat agcgttttct catcaaggcg
      601 tatttcccaa gcagcacgtt ctgttcgtct tttaatgtta tgttgggaat cagttgttct
      661 cccattggaa tgtattctag tttcatcatg atggctcctt tcagtatgtc ttatttcttt
      721 ttcatgttca ttgtatatcg gctgtgagct tagttcaaat gtgcgattat gacttctgat
      781 acattccagc accgattttc taatttcacc gatcagcatt tgtgtcatat ctctagttgg
      841 aacaccgatt gcatttaatg cttgtggcga attaaactgt gaaatcaaac gcaaggcatc
      901 atcactaata taatcatctg caatgattcc acatcttgtt aatgtggcat atgaaatact
      961 gctttttaaa agagcagtat agattaactg taaatttaat tcatcaagtt cctctaaaaa
     1021 ggtatcattc acataatatt taagttcaga aaaataatca ctcataccct catcaacaat
     1081 aacttcactt gcttggataa ttgtatcggc taagttcata gaatcacttt tagataaact
     1141 gaatgtatct atcaagttat tgattacttc ttcttcatat tccggtttca ttgaccatat
     1201 aggaacagga cggtatttat ttgtttttct cgtatcactt atatcaaaat aatagcgtag
     1261 tcttgcttga ccgttatgat gaggatcaaa tactgcgata ccttttgatt taggtttgat
     1321 ccatagacca tattttttat tccaatcatc catttctaaa acagcgattg catttggtct
     1381 ttgggcatag atcatgacca tatcatcaaa agacaaacga taatttttac aagaagttga
     1441 gagaaattgt ttccatgtaa attcatcatg agcaatcttt tttaaagtct gttgatacag
     1501 ttctgttgcc tgataataat tcatcgccat gtgtatcaca cctccctttt agcaatgttt
     1561 ttttatcttg atggctcttt atttttcatt ctttgaagct gtgcaaggaa tttaggtact
     1621 tcaacaaagc caaaactatc tacataatag gcttttcttg ttccataatc attgataaca
     1681 atgacatcac ttacactgac agaacgcatc ttttgagcaa aaggacgata atcaagatta
     1741 tgcttataga aaatactttc caaagaggtt ttatctgcta acattcccat atacattttt
     1801 tcataatgtt tggcttgaaa atgtttttct gcataagagt atcgcataaa aagataatcc
     1861 ctgcctttat cagaggtgat ttgataaatc tcataagttc ttgatgaacc atgttccgga
     1921 tagaaaatac agctgttttt ctctacaagt tcagcaaact ctttgaaaga aaaacgagca
     1981 ttttttaaat agaaactgcg ttcatcaatg tagttgcatg taatcacaac cggcttgttg
     2041 tccaatgcct gcaagacgat
//
LOCUS       DAOMTA010000594         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2681_length_2059_cov_2.692615, whole genome shotgun sequence.
ACCESSION   DAOMTA010000594 DAOMTA010000000
VERSION     DAOMTA010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_2059_cov_2.692615"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1158
                     /locus_tag="PLK68_13330"
     CDS             28..1158
                     /locus_tag="PLK68_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRM91427.1"
                     /translation="MDALNEIKRWRQELHQIPELGLQEFKTSKYLKDELTKMGYEPIS
                     ILDTGVLVYLDNHQDKTLAFRSDMDALKISEQTNCSFKSCHNGYMHACGHDGHMAALL
                     GLAKKLKEQPLKWKHNILLIFQPAEESPGGAKLIVEAGILKQYNVRAIFGLHLMPTIE
                     AGKIACRPGPLMAQNGELDVTITGKSAHAGLYHLGIDSIMIASQIICQYQSIISRVIA
                     PVESCVINIGEIQGGTVRNIVADQTTFKGTVRTYSETVFKKITDTMEAINHGMEQTYG
                     CTIEFSCPPMYPPVLNDYDLYRQFVRLTDENYEELKEPLMLAEDFSFYQKEVPGIFFY
                     VGTKTPKYFSGLHTETFNFDEEVLMQAVELYYRLADKIKLGD"
     gene            1161..>2059
                     /locus_tag="PLK68_13335"
     CDS             1161..>2059
                     /locus_tag="PLK68_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM91428.1"
                     /translation="MDYFDRANNAFMKAYGRFDVTFDHGNGVYLYDTNGKKYLDFYSG
                     IGVNSFGYDYQLYTDAMCQQMHRLMHISNYFNSVETIEAAEAVIKATQLDQVFFTNSG
                     TEATEGALKLARKYYYEKHGKADSEIISLNHSFHGRSTGAVTLTGTPAYQTAFGPLIT
                     GVKYGDINDLDSVAKLITPRTAAIILEPVQGEGGIHVCTQEFMKGIRQLCDKHEIVMI
                     LDEVQCGMGRTGTIMTYFQYGIMPDIVCLAKGIGAGFPMGAFVANKKIGDALKPGDHG
                     STYGGNPMAGKACKTVFKILEET"
BASE COUNT          697 a          278 c          445 g          639 t
ORIGIN      
        1 atttaagaaa aatttaggag atcagttatg gacgctttaa atgaaattaa aagatggcgt
       61 caggaattac atcaaatccc tgagctaggt ttacaagaat ttaagacttc aaaatatctt
      121 aaagatgaat taactaagat gggatatgaa ccaatttcaa tcttggatac aggggttctt
      181 gtttatcttg ataatcatca agataaaacc ttggcttttc gtagtgatat ggatgcattg
      241 aaaatatccg aacaaactaa ttgtagtttt aaatcatgtc ataatggtta tatgcacgca
      301 tgtggtcatg atggtcatat ggctgcttta cttggattag ctaaaaaact aaaagagcag
      361 cctttaaagt ggaagcataa tatcttattg atatttcagc cggcagaaga atcaccaggt
      421 ggtgcaaagt taattgttga agctgggatt ttaaagcagt ataatgtaag agcaatattt
      481 ggtttacatt taatgccgac aatcgaagca ggaaagattg cttgtcgacc gggaccattg
      541 atggctcaaa atggtgaact tgatgtgaca ataactggca aaagtgctca tgcagggtta
      601 tatcatttag gaattgattc aatcatgatt gctagtcaaa taatttgtca atatcaaagt
      661 attatttctc gagtaatagc acctgttgaa agctgcgtaa ttaatattgg agaaattcag
      721 gggggaactg tgcgaaatat cgttgctgat caaacaactt ttaaaggaac agttagaact
      781 tacagcgaga cagtttttaa aaaaattaca gatacgatgg aggcaattaa tcatgggatg
      841 gagcaaacct atggctgtac cattgaattt agttgcccgc caatgtatcc accggtttta
      901 aatgattatg atctatatcg gcaatttgtt agattaactg atgagaatta tgaggaatta
      961 aaagagccgt taatgttagc tgaagatttt tctttctatc aaaaagaggt accaggaata
     1021 ttcttttatg taggaactaa aacaccaaaa tattttagcg gtttacacac agaaacattt
     1081 aattttgatg aagaagtctt gatgcaggca gtagaattat attatcgtct agcagataag
     1141 attaaattgg gggactaaaa atggattatt ttgatagagc taataatgct tttatgaaag
     1201 cttatggtag atttgatgtt acttttgatc acggtaatgg agtttattta tatgatacaa
     1261 acggtaaaaa gtatttggat ttttattctg gaataggtgt taattcattt ggctatgatt
     1321 atcagctgta tacagatgca atgtgtcaac agatgcaccg tttaatgcac atatcaaatt
     1381 attttaatag tgttgaaaca attgaggctg ctgaagcggt aattaaagca acacaacttg
     1441 atcaagtatt ttttactaat agtgggacag aagcgaccga aggggcgttg aagttagcgc
     1501 gtaaatatta ttatgaaaaa catggaaagg ctgatagtga aattattagt ttgaatcatt
     1561 cttttcatgg acgttctaca ggagcggtta cattaacagg aactcctgca tatcaaactg
     1621 catttggacc attaattacg ggtgtaaaat atggcgatat taatgatttg gattcagtgg
     1681 ctaagttaat caccccaaga acagctgcga ttattttaga acctgttcaa ggggaagggg
     1741 ggatccatgt ttgcacccaa gaatttatga agggaattcg acaattatgt gataagcatg
     1801 agattgtaat gattcttgat gaagttcaat gtgggatggg acgaacagga acgatcatga
     1861 catatttcca atatggaatc atgcctgata ttgtttgtct agctaaagga attggggctg
     1921 gtttcccaat gggagcgttt gtagcaaata aaaaaatcgg agatgcttta aaacctggtg
     1981 atcatggatc tacttatggt ggtaacccta tggctggaaa agcttgtaag acagtcttta
     2041 agattttaga agaaacgca
//
LOCUS       DAOMTA010000595         2055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2686_length_2055_cov_2.928500, whole genome shotgun sequence.
ACCESSION   DAOMTA010000595 DAOMTA010000000
VERSION     DAOMTA010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2686_length_2055_cov_2.928500"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..472
                     /locus_tag="PLK68_13340"
     CDS             149..472
                     /locus_tag="PLK68_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRM91429.1"
                     /translation="MTSININKEKFGQLIHKEKPVLVDFWAPWCGYCRRIGAAYEKIS
                     EEYSDILVAGKVNIDEEPQIAEAEKIEIIPTLVLYRDGKAIDSIVAPESKIMIEKFIN
                     EALEK"
     gene            488..1414
                     /gene="trxB"
                     /locus_tag="PLK68_13345"
     CDS             488..1414
                     /gene="trxB"
                     /locus_tag="PLK68_13345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRM91430.1"
                     /translation="MNENHVYDMIIVGGGPGGYTSALYAARAGFDTIVLEKLSAGGQM
                     SLTWQIDNYPGFENGIDGFSLAEKMQKQAEKFGAKSEYAEVFNMDLTGKLKRVETSSG
                     IYIGKTVVIATGANPRELGLAKEKELIGHGVAYCASCDGMFYKDKIVVVVGGGNSAVS
                     DAVLLSRIAKKVIIVHRRDTLRATKIYHDQLMKTENIEFRWNNTVNELIYGERLTGVR
                     LKDTVTGEENIIECDGLFVSIGRKPTTDFLDNQIELDNKGYIVAGENTETSIPGVYAV
                     GDVRTKLLRQIVTAVADGAMAVHMAEKYMGKM"
     gene            1846..>2055
                     /locus_tag="PLK68_13350"
     CDS             1846..>2055
                     /locus_tag="PLK68_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM91431.1"
                     /translation="MYIEHPYFYEGKYYANIDGEMIEITKEVAYAMNNFYRSSKAKKV
                     EIKNELGEVVDKMLREVPYSGQSIDG"
BASE COUNT          735 a          258 c          492 g          570 t
ORIGIN      
        1 tgagaagagt caattatata actgacatat gctgtaaata gaagtaaaaa agcgtaataa
       61 agtaaatcaa cttttctgtg atatcatcac ggaagcaagc acttatttgg ggtatattaa
      121 gttcaaatag gagaaggagt tgaaaaatat gacatctata aatataaata aagaaaagtt
      181 tggacaatta attcataagg aaaaaccggt tttggttgat ttctgggcac cgtggtgtgg
      241 ttattgccgt agaattggag cagcttatga aaaaataagt gaagaataca gcgatattct
      301 tgttgctgga aaagtaaata ttgatgaaga accacagatt gcagaagctg aaaagattga
      361 gataattcct acgctggttt tatatcgaga tggaaaggca atagattcta ttgttgcacc
      421 tgaatcaaaa ataatgatag agaaatttat taatgaagca ttggaaaaat aaaaggagga
      481 ctgctgtatg aatgaaaatc atgtttacga tatgattatc gtggggggag gacctggagg
      541 gtatacttca gcattatacg cagccagagc aggatttgat acaattgttt tagaaaaatt
      601 atctgcaggt ggtcagatgt cattgacctg gcagattgat aactatcctg gttttgaaaa
      661 tggaattgac ggattttcat tggcagagaa aatgcaaaaa caggcggaaa aatttggcgc
      721 taaaagtgaa tatgctgaag tttttaatat ggatttaaca ggaaagctta agagagtaga
      781 aactagttcg ggaatttata ttgggaaaac agtagtgatt gctactggag caaatccaag
      841 agaacttggt ctcgctaaag aaaaagaatt gataggtcat ggggttgctt attgtgcctc
      901 ctgtgatggt atgttttata aagataagat cgtggtggtt gttggtggtg gaaattctgc
      961 tgtatcagat gctgttcttt taagtcgaat tgctaagaaa gtcatcattg ttcatcgaag
     1021 agatacatta cgtgcaacga aaatctacca tgaccagctg atgaaaactg aaaatataga
     1081 gtttcgatgg aacaatacag taaatgaact gatttatgga gaacgattga ctggtgtgcg
     1141 gttgaaagat acagttacag gagaagaaaa cattatagaa tgtgacggcc tgtttgtgag
     1201 tataggaaga aaaccgacaa cagatttttt agataatcag atagaactgg ataataaagg
     1261 atatattgtg gcaggtgaaa acactgaaac gagtattccg ggtgtttatg ctgtaggaga
     1321 tgtcagaaca aagttacttc gtcagatagt aacagctgtg gcagatggtg ctatggcagt
     1381 acatatggca gaaaaatata tggggaaaat gtaaagtgaa ctccgccttt tggcatagga
     1441 attgttttgt taaattaaat aatgaaacag aaaatagtca ttttgataga acatattaac
     1501 tataaataat cagaatttga atagtgaact ttttacactg aaatatgtac gattctaata
     1561 atgacatgaa cttatgggaa cgtattgcgg atactttgtg aactttatcc tgatacaatg
     1621 attatgctga tgaaagaagg catagataac tgaatacaag aaagagacga aagcctcagt
     1681 agttaagaaa aaactactgg ggcttttttt cgtgcctgaa tttttcagcc aataactgcg
     1741 ggcgtatccc ggcaggataa aggcggagca ggcatgattc taaacagagg tccgccttct
     1801 ggatttgaaa cgagcaacaa gatttcaaat tcaggaggaa cggacatgta tattgaacac
     1861 ccatattttt atgaaggaaa gtattacgca aatattgatg gagagatgat tgagattaca
     1921 aaagaggttg cgtatgcgat gaacaatttt tacagaagca gcaaggcaaa aaaagttgag
     1981 atcaagaacg aactgggtga ggtcgtggat aagatgctgc gggaggtacc ttacagtgga
     2041 cagtccattg atggg
//
LOCUS       DAOMTA010000596         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2690_length_2054_cov_1.722361, whole genome shotgun sequence.
ACCESSION   DAOMTA010000596 DAOMTA010000000
VERSION     DAOMTA010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_2054_cov_1.722361"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1023)
                     /locus_tag="PLK68_13355"
     CDS             complement(<1..>1023)
                     /locus_tag="PLK68_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM91432.1"
                     /translation="FTNGDWLDASLFALSVAVGLTPEMLPMIVTTSLAKGSLAMAKEK
                     TIIKNLNSIQNLGAIDILCTDKTGTLTQDEVILEFPLDVHGKIDLRVLRHAFLNSYYQ
                     TGLNNLMDKAIINSTLAEQDNDSSLKDLTNKYEKIDEIPFDFQRRRMSVIIQDQDGKV
                     PMVTKGAIEEMLSVCRYVEYLGKVWPLTQKLEKIIINQVEQLNEKGLRVLGVSQKTDQ
                     DLVQKCTVSDEKDMVLIGYLAFLDPAKESTAPAIKALKEHGVATKILTGDNEKVTKAI
                     CQKVGLNVENILLGQDVAKMGLDKLKEVVETTTIFAKLSPEQKALIIKVLKENGHSVG
                     YMGDGI"
     assembly_gap    1024..1033
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1034..1900)
                     /locus_tag="PLK68_13360"
     CDS             complement(<1034..1900)
                     /locus_tag="PLK68_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM91433.1"
                     /translation="MRRFNSINERLVFASENKTNQVLDSFETNKMGITNETVKHHSDE
                     FGKNIITKQKKDSILKKIFNAFINPFTAILLILAVVSLFTNVIFAETSEKDPTTVIII
                     VVMVMISGILRFIQEQRSGSAAEKLIAMISNTTNIKRYGQEAKEIPIDEVVVGDIVYL
                     SAGDMIPGDLRIIEAKDLFISQASLTGESEPVEKFAIETTVGTNVLEAQNLAFMGSDV
                     ISGSAVGVVIATGDETMLGRISIDLNKKRELTTFEIGINSVSWLLIRFMLVMVPVVLF
                     INVPVPIFPALSC"
BASE COUNT          642 a          410 c          231 g          761 t
ORIGIN      
        1 ttaataccat cgcccatata tccaacgcta tgtccatttt cttttaaaac tttaataatc
       61 agtgcctttt gttccggtga taattttgca aaaattgttg ttgtttctac aacttccttc
      121 agtttatcca agcccatctt tgcaacatct tggcctaata aaatattttc aacattcaat
      181 cctacctttt gacaaattgc tttagttact ttttcattat ctcctgtcaa aatttttgtt
      241 gctacaccat gttccttcaa tgcttttatc gctggggctg ttgattcttt tgcaggatct
      301 agaaaagcta aatatccaat taaaaccata tctttttcat cactaacggt acatttttgt
      361 actaaatcct gatctgtttt ttgactcact cccaagaccc gtaaaccttt ttcattaagt
      421 tgttcaactt gattgataat tattttctct aatttttgtg ttaacggcca aactttacca
      481 agatactcaa catatctaca aacactgagc atttcttcaa ttgctccttt tgtaaccatc
      541 ggtactttac catcttgatc ctgaataata acagacattc ttcgtcgttg aaaatcaaat
      601 ggaatctcat caattttttc atatttgttt gttaaatctt ttaagctcga atcattatct
      661 tgttcagcca acgtactatt aataattgct ttatccatta gattatttaa tcctgtttga
      721 taataactat tcaaaaatgc atgtcgcaaa actcttaaat caattttccc gtgaacatct
      781 aaaggaaatt ctaaaattac ttcatcttgt gttaaagttc ctgttttgtc tgtgcaaaga
      841 atatcaatag caccaagatt ttgaattgaa tttaaatttt taattatagt tttttcctta
      901 gccattgcaa gagagccttt agcaagacta gttgtgacaa tcatcggaag catttcaggt
      961 gtcaaaccta cagcaacact taatgcaaat aaacttgcat ctaaccaatc tccattagta
     1021 aatnnnnnnn nnnacaagac aaagcaggaa atataggaac aggaacatta ataaataaaa
     1081 caacaggtac cataactaac ataaaacgaa tcaaaagcca cgatacagaa ttaattccaa
     1141 tttcaaaagt tgttaattct ctctttttat ttaaatcaat agaaattctt cccagcatag
     1201 tttcatcacc tgtcgcaatt accacaccga cagcacttcc gctaattaca tcacttccca
     1261 taaaagccag attttgtgct tcaagcacat tcgtccctac agttgtttca attgcgaatt
     1321 tttcaactgg ttcactttca cctgttaaac ttgcttggct aataaataaa tctttagctt
     1381 caataattct caaatctcca ggaatcatat ctcctgcgct caaatataca atatcaccaa
     1441 caactacttc atcaattgga atctcttttg cttcttgtcc atatcgttta atatttgtag
     1501 tatttgaaat cattgcaatc agcttctctg ctgcactccc acttctttgt tcttgaataa
     1561 agcgtaatat tccagatatc attaccataa ctacaataat gattactgta gttggatcct
     1621 tttcactagt ttctgcaaaa atcacattag taaataaaga tactactgct agtattaata
     1681 aaattgcagt aaatggattt ataaaagcgt taaatatttt ttttaatata gaatcttttt
     1741 tttgtttagt aataatattt ttaccaaatt catcactatg atgtttgact gtttcattcg
     1801 tgattcccat tttatttgtt tcaaaactat ctaaaacttg attagtttta ttctcactcg
     1861 caaaaactaa tctctcatta attgaattaa atcttctcat tgttataccc tccaaattaa
     1921 attttagagt atgacaaaac tatcctaaag aatggcatcg ccattattcg ggacttataa
     1981 acatcattgt cataccggcc atgctcttcc attctttttc tcctttatgt gaattcaata
     2041 ttttattcct gttc
//
LOCUS       DAOMTA010000597         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2703_length_2050_cov_2.260150, whole genome shotgun sequence.
ACCESSION   DAOMTA010000597 DAOMTA010000000
VERSION     DAOMTA010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_2050_cov_2.260150"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLK68_13365"
     CDS             complement(<1..213)
                     /locus_tag="PLK68_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRM91434.1"
                     /translation="MIKNIIGGIAVGIANVIPGVSGGTMMVILGIFNRMMDAISGIFK
                     KENPNRKEDIIFIFQVLVGAGVGIIGF"
     gene            complement(278..652)
                     /locus_tag="PLK68_13370"
     CDS             complement(278..652)
                     /locus_tag="PLK68_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HRM91435.1"
                     /translation="MKDELGLGYCGLVCGLCSENESCVGCKKGGCPEKDFCKNYQCCT
                     KSGYDSCCQCPDFPCQDSILHKLRIRTFCKFIGQYGEEKLIVCLRKNESNGVIYHYPN
                     SHLGDYDLESEEAIYHLILKGK"
     gene            complement(662..1981)
                     /locus_tag="PLK68_13375"
     CDS             complement(662..1981)
                     /locus_tag="PLK68_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRM91436.1"
                     /translation="MKQQAMFKNMSNEPLANRLRPTTLTEYVGQRHLIGPGKILYQLI
                     NSDVVPSMVFWGPPGVGKTTLARIIANQTKAKFINFSAVTSGIKDIRAVMKQAQEVQD
                     LGEKTIVFVDEIHRFNKAQQDAFLPYVEQGSIILIGATTENPSFEINSALLSRCKVFV
                     LKALTTDDLFGLLHYALISPKGFKDQNVMIDDDLLYMIAGFSNGDARVALNTLEMAVL
                     NGAITHDRIVVDKETIEQCINQKSLLYDKKGEEHYNIISALHKSMRNSDIDASIYWMS
                     RMIEAGEDPLYVARRLIRFASEDIGMADSRALEIAVATYQACHYNGMPECNVNLAHCV
                     TYMALAPKSNALYKAYERAKHDALNTIADPVPLQIRNAPTKLMKELNYGKGYQYAHDY
                     DDKITNMQCLPDNIKDRRYYFPTNQGTEAKVIKRMEQIEYLRKHHKD"
BASE COUNT          663 a          437 c          257 g          693 t
ORIGIN      
        1 aaatccaatt attccaactc cagcgccaac caaaacctga aaaataaaga taatatcttc
       61 tttgcgatta ggattttctt ttttaaagat tccactaata gcatccatca tgcgattaaa
      121 gatacctaga attaccatca tggttccacc actaacgcct ggaataacgt tagcaatacc
      181 aacagcgatt ccgccaatta tgttttttat cattgaaact tctcctttaa atacctcatc
      241 attataataa aatatcacca ttatcacaag cagtatttta tttccctttt aaaatcaagt
      301 gataaattgc ttcttcactc tctaaatcat aatctcctaa atggctgtta ggataatggt
      361 aaattactcc attagactca tttttcctta aacaaacaat taatttttct tccccatatt
      421 gtccaataaa tttacaaaat gttctaatcc gtaacttgtg taaaatacta tcctgacaag
      481 gaaaatcagg acattggcaa caactatcat atcctgactt tgtgcagcac tgataatttt
      541 tacaaaaatc cttttctgga cacccacctt ttttacaacc aacacagctt tcattttcac
      601 tacacagccc acaaacaaga ccacaatatc ctaatcctaa ttcatccttc atatttaatc
      661 cctaatcctt atgatgcttt cgtaaatatt caatttgttc catccgttta atcacctttg
      721 cctctgttcc ttgattagta ggaaaataat aacgacgatc cttaatatta tctggtaaac
      781 actgcatatt tgtaatttta tcatcataat catgcgcata ctgataacct ttaccataat
      841 ttaattcttt cattaattta gttggcgcat tgcgaatttg taaaggaacg ggatcagcaa
      901 ttgtattcaa agcgtcatgt tttgctcgtt catacgcttt atataacgca ttagatttag
      961 gtgccaaggc catataagtt acacaatgag ctaaattaac attacattct ggcatgccat
     1021 tataatggca tgcttgatat gttgctactg caatttccaa agcacgacta tcagccattc
     1081 caatatcctc tgaagcaaat cgaatcagcc tccttgctac atatagagga tcttcaccag
     1141 cttcaatcat tcgtgacatc caataaattg aagcatcaat atcactatta cgcattgatt
     1201 tatgcaaagc actaataata ttataatgtt cttcaccttt tttatcatat aataaagatt
     1261 tttgattgat acattgttca atcgtttctt tatccactac gattcgatca tgagtaattg
     1321 ctccatttaa gactgccatc tctaaagtat ttaaagcaac tcgagcgtca ccgttagaaa
     1381 aaccagcaat catgtataat aaatcatcat caatcataac attttgatct ttaaaccctt
     1441 tcggacttat cagcgcataa tgcaataaac caaataaatc atcggttgtt agtgctttta
     1501 aaacaaaaac tttacatctt gataataagg cagaattaat ctcaaatgaa ggattttcag
     1561 tagtagcccc aattaatata atactcccct gttcaacata tggtaaaaaa gcatcttgtt
     1621 gagctttatt aaagcgatga atttcatcta caaagacgat tgttttttcg cctagatcct
     1681 gaacttcctg tgcttgtttc ataactgccc gtatatcttt aatgccgcta gtcaccgcac
     1741 taaaattaat aaacttagct tttgtttgat ttgcaataat gcgtgctaaa gttgttttcc
     1801 caactcccgg tggtccccaa aaaaccattg agggaacaac atcactattg attagctggt
     1861 acaaaatctt tcccggtcca attaagtgcc tctgtcctac atattcagtc aaagttgttg
     1921 gtcttaggcg atttgccagt ggttcatttg acatgttttt aaacattgct tgctgcttca
     1981 tcatcatcac ttccatttcc ttataattat aactaattcc cgctttaaaa gcaaaaaaag
     2041 aaacagttgt
//
LOCUS       DAOMTA010000598         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2709_length_2048_cov_2.003512, whole genome shotgun sequence.
ACCESSION   DAOMTA010000598 DAOMTA010000000
VERSION     DAOMTA010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2709_length_2048_cov_2.003512"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..1620)
                     /locus_tag="PLK68_13380"
     CDS             complement(304..1620)
                     /locus_tag="PLK68_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM91437.1"
                     /translation="MKKFVSILLAVVLIFSVLTCVGCEADKKEKNTITYYHYEPYVSP
                     VSILIYQYNEKCKNVQNRINIVEFDNIEKMTSQLTTELMAGKGPDIITSGELSLLSLS
                     TEKLIKQGVFANIDDIISNSEDKDKLKLSDYNSNILDAGVYDGKRYFIPVTFEPQILL
                     SSSSKLSKYLDNKQPDLTYGNIKELSGNINNDDNNQALFSGVEDYKNVFLNLIDENVD
                     FYSQKYDFDSDDFRKSVTSLKELYSSNEKNEDNIVPMLSSNGQIELFALYDEIADIEN
                     DNDEPIILNTPNKDGESTGVITDAIFISNNSDNKEKALEFIEYALSEKVQSAFCGANI
                     EEYLPTTSYGSGWEYPVNNESYSKLFDKAKEVKYINNTANISEANFMYAKDYVDNIKS
                     YRLYGLYNYYNTEVIGDIVDKFLSDEIDENKFLKELKSRTEIYLDE"
BASE COUNT          666 a          352 c          261 g          769 t
ORIGIN      
        1 cactgagtta aattcacttc attaataatt gaaagtaaca tataaaccct ttgcaattcc
       61 ctttgcaggg tcaaaaacta ctacagtatc atccttaact ttagttataa ccacataatg
      121 caatatggaa tcgtcaatca ctacatgtgc aataacaggt ataggtaatt tagaggttaa
      181 tgattcctta tcagcttcaa caaccttttg ctgaaaacca aaatgttcaa ttacctttac
      241 aagaccgtat gcactagtac cttgtcgatc cgtacctgca atatccctaa tttttgcata
      301 acactattca tctaaataaa tttcagttct tgatttaagc tctttaagaa atttattttc
      361 atctatttca tcggataaaa atttatctac aatatcacct ataacctctg tgttataata
      421 attatataat ccataaagtc tatagctttt tatgttatca acgtaatcct ttgcatacat
      481 aaaattagct tctgaaatat ttgcagtgtt attaatatac tttacctctt ttgctttatc
      541 aaataattta ctgtaagatt cattgttgac gggatattcc cagccacttc cgtaagaggt
      601 tgtcggtaga tattcctcta tattcgctcc gcaaaaagca ctctgcacct tttccgataa
      661 cgcatactca ataaactcaa gagctttttc tttgttgtcg gaattattgc taataaaaat
      721 tgcatcggtt ataacacccg tggactcacc gtctttattc ggtgtattca aaattatggg
      781 ctcgtcatta tcattttcaa tgtctgcaat ttcatcatat aacgcaaata attcaatctg
      841 accgttcgat gataacattg gcactatgtt atcttcattt ttctcattac tgctgtatag
      901 ttcttttagt gaggtgacag actttctaaa gtcatcgcta tcgaaatcat atttttgaga
      961 atagaagtca acattttcat ctattaaatt aagaaaaaca tttttataat cctcaacgcc
     1021 ggaaaacaga gcttgattat tatcatcatt attaatattt ccgctcaatt ctttgatgtt
     1081 accgtatgtt aaatccggtt gtttgttgtc taaatatttt gataatttgc ttgatgagga
     1141 gagtaaaatc tgaggctcaa atgttacagg aataaaatat ctttttccat cgtaaacacc
     1201 cgcatcaaga atattagaat tataatcgga taatttcaat ttatccttgt cttcgctatt
     1261 agaaataata tcgtcaatat ttgcaaatac accctgttta ataagttttt ctgttgataa
     1321 tgataataga gataattcac cagatgtaat aatatcagga cctttccctg ccataagctc
     1381 agttgtaagt tgacttgtca ttttttcaat attatcaaac tcaacaatat tgattctatt
     1441 ttgaacattc ttacattttt cattatattg atatattaaa atagaaactg gtgatacata
     1501 cggctcataa tgataataag taattgtatt cttctctttt ttgtctgctt cacatccgac
     1561 gcaggttaaa acagaaaata ttaatacgac tgccaataaa atacttacaa attttttcat
     1621 cagcttactc ctctgtataa taataattta ctttaaacaa aagtttattg atacaactat
     1681 gtcaaaattt acggataaca tgttaaaagt atataggaaa tgatgttgct atttaattat
     1741 tatccaagaa atacaacact tgattttcca gttacctgat ataatcctag acctgaactg
     1801 tacgtatttg cataatttag tgcagctttt gtatgtgctg ttgcattagt gcatttttta
     1861 ctccgtcctt aattttactt gataatgaat ttttgttttt cgttgtaatc aaccttctta
     1921 ttataaattt atcaaaaaaa tatttgtgaa tgttgatttt tgcaaaaatg gtaataaatc
     1981 cgacaaaaat tgcactctat aattcttatt tagggaagca gctttaaacc ttatttaaat
     2041 ttttcgat
//
LOCUS       DAOMTA010000599         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2722_length_2040_cov_2.190428, whole genome shotgun sequence.
ACCESSION   DAOMTA010000599 DAOMTA010000000
VERSION     DAOMTA010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_2040_cov_2.190428"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..361
                     /locus_tag="PLK68_13385"
     CDS             5..361
                     /locus_tag="PLK68_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91438.1"
                     /translation="MRKKEDKYDFRAFGLAIKKARMKKGLTREQVGAMIEIDPRYLTN
                     IENKGQHPSLQVFYDLVSLLEVSVDEFFLPASDLVKSTRRRQLEKQLDSLSDKDLIIM
                     ESVADGIIKSKEVEDK"
     gene            complement(428..619)
                     /locus_tag="PLK68_13390"
     CDS             complement(428..619)
                     /locus_tag="PLK68_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HRM91439.1"
                     /translation="MKSKCKWILCPVCGNKTRLQIRNDTELKNFPLYCPKCRQESLID
                     VNKFKIRVITEPDAKTQSR"
     gene            complement(632..1996)
                     /locus_tag="PLK68_13395"
     CDS             complement(632..1996)
                     /locus_tag="PLK68_13395"
                     /inference="COORDINATES: protein motif:HMM:NF040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HRM91440.1"
                     /translation="MNNLSLFTGNTINVLSIINTALQHNYISSARADSLYCEMETLSN
                     DLVMYFNHGTSTSLDKNDFIRIWDTINYMFIHGFNEDNQSYYCLKTKNIRDYFNSGLE
                     CVKKDILLIQEILEQLKNQKLPINNERYSSILYEQIPDFLQSLKKYYAIFNYCYIAGD
                     LDYPLYDGLPLYHDMYNLKGSDLALHYLKCFSIENAFCYEFYKELPELVQQFEDFKGI
                     PIYYLGINIFELVLYQDICHILLYNSSGILLEETDIGRIKNRLENSVDMSKDIEYALK
                     LICSSLPDEMSKYIWRFKNEIKKKFESFINDDFELLIYRKAYKTSNVILLNSIQSNQH
                     FLKILTHIQSLTDIEEKIIYLQNIKINIYDLFDLLENDIFIGNEYNLYFSTLTPNDIA
                     IIIKALIPDLGVFNQKCSLDDELYSELDCGVEWKKKLIDFLKKYSILQKKEVEYALNN
                     IKII"
BASE COUNT          679 a          318 c          281 g          762 t
ORIGIN      
        1 acatatgcgt aaaaaagaag ataaatacga ttttagagct tttggtcttg ctatcaaaaa
       61 agctcgtatg aaaaaagggt tgacccgtga acaggtggga gcaatgattg agattgaccc
      121 tcgttaccta acaaatatcg agaacaaagg gcagcaccca agtttacagg tattctatga
      181 ccttgtttca ttactggaag tttccgttga tgaatttttc ctgcctgcgt ctgatctggt
      241 gaaaagcacc agaagacgac agttggaaaa acagcttgat agcttatccg ataaagattt
      301 aattatcatg gaaagcgttg ctgacggaat cataaaatct aaagaagtgg aggacaagta
      361 atccttcgct ttttagtttt caaataaaat aagccgataa cccaagaacc ttttttcatt
      421 ctcggtgtta tcggctctgc gtcttagcgt ctggctctgt tattactcta attttaaatt
      481 tatttacatc aattagactt tcctgtcggc atttcggaca atacaatgga aaatttttta
      541 actctgtatc atttcttatc tgtagtcttg tcttattacc acatacagga cataaaatcc
      601 atttgcattt agatttcatt tcattctctc cttatatgat ttttatatta ttaagtgcat
      661 attcgacttc ttttttttgt aaaatactgt atttctttaa aaaatctatt aattttttct
      721 tccattcaac accacaatct aattcactgt ataattcatc atctaaagaa catttttgat
      781 taaatactcc caagtctgga attagagctt ttattattat tgctatatca ttaggtgtaa
      841 gtgttgaaaa gtataaatta tactcgtttc caataaaaat atcattttcc agtaagtcaa
      901 ataaatcata tatgtttatt ttaatatttt gaagatatat gattttttct tctatgtccg
      961 ttagcgattg aatgtgtgtt aatattttca aaaagtgttg attactttga atagaattaa
     1021 gtagaataac atttgaagtt ttatatgctt ttcgataaat tagtaattca aaatcatcat
     1081 ttataaatga ttcaaatttt ttctttattt catttttaaa tctccaaata tacttactca
     1141 tttcatctgg taatgaggaa cagataagtt tcaaagcata ttctatatct tttgacatat
     1201 ctactgaatt ttctaatctg ttctttattc tgccaatatc cgtttcttca agaagtatgc
     1261 ccgaactatt gtacaaaaga atatgacata tatcttgata gagtactaat tcaaaaatgt
     1321 tgattcccaa ataataaata ggaataccct tgaaatcttc aaactgttga accaattcag
     1381 gcaattcttt gtaaaattca taacaaaaag cattttcaat ggaaaagcat tttaaataat
     1441 gtaacgctaa gtcacttcct ttaagattat acatatcgtg atatagcggc agcccatcat
     1501 acaaaggata atctaaatct cctgcaatat aacaataatt gaatatggca taatacttct
     1561 ttaatgattg caaaaaatca ggtatctgtt catagaggat actggaatat ctttcattat
     1621 tgattggtag cttttggttc tttaattgtt ctaaaatttc ttgaattaat aaaatatctt
     1681 tttttacaca ttctaaacca ctgttaaaat aatctcttat atttttagtt ttcaagcaat
     1741 agtaagattg attatcttca ttaaatccat gaataaacat ataatttatg gtatcccaaa
     1801 tccgaataaa gtcgttttta tctagtgagg tactagttcc atgattaaag tacattacca
     1861 aatcatttga tagtgtttcc atctcacaat ataaactatc agctctggca gatgaaatat
     1921 aattatgttg taaagcagtg ttgataatag aaagcacatt tattgtatta cctgtaaata
     1981 gggatagatt gttcaactct atattgtcca ccttctttcg ctatgattct tattgttctt
//
LOCUS       DAOMTA010000600         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2723_length_2040_cov_2.177834, whole genome shotgun sequence.
ACCESSION   DAOMTA010000600 DAOMTA010000000
VERSION     DAOMTA010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_2040_cov_2.177834"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLK68_13400"
     CDS             <1..294
                     /locus_tag="PLK68_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91441.1"
                     /translation="DNIDIYCLLGILNSKLMNWFFRCFSTNSNVNGYEIDNLPIPQLD
                     NQLQNKISELVLQILHQSNVSQIKIKEIEKRIDIIVYKTFELNNNEIKLINES"
     gene            complement(502..1686)
                     /locus_tag="PLK68_13405"
     CDS             complement(502..1686)
                     /locus_tag="PLK68_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HRM91442.1"
                     /translation="MGNPPYITYRGKEKTSIKDDELKILITHYKKSAEYKVNSYALFI
                     EEGLTLCRTDGNLSYIIPGSILQNSYLKKIRRFLLTENQIKEIVMFDNRIFDAVTDSL
                     ILRCNNGYKNNNIMSYKRVKDLSFKDVDQQFIKTEIWNNIKTSFVIDVKTNATDCNIF
                     FKMESLGLQLGDIYNVYVGIVANGIKKFLYKTKETDSFKKYLQGKHIAPYIIKYNSIY
                     INFQPNLLHSNTDTSVYEQSEKILVRKTGNVLLAVIDNEQYYTDQSIYNLYLKTDNYN
                     NKVCCAILNSKLLNYYFNKKLITNAEVFPYIKGLHLKQLPLPLLSDKNAVDYNCNLEF
                     KISQEVDRITSIKSKDPQANIEKEQKEIDILVYHLYGLTYKEVLTIDPKFEINKDSYM
                     NI"
     gene            complement(1717..>2040)
                     /locus_tag="PLK68_13410"
     CDS             complement(1717..>2040)
                     /locus_tag="PLK68_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91443.1"
                     /translation="IKDNYRLGEDIQSKVLAFAFGLSAEIERNLISQRTKEALARKKA
                     KGVVLGRKLGSKNSYTKLTGKEDYIHKWINDGMSKAKIARKLHVNPSTIFRFLQRTDG
                     NMAIS"
BASE COUNT          661 a          306 c          272 g          801 t
ORIGIN      
        1 gataatattg atatatattg tttattagga atattaaatt caaaattgat gaattggttt
       61 ttccgctgtt tcagtacaaa tagcaatgtt aatggatatg agattgataa tcttccaata
      121 ccccaattag ataatcagtt acaaaacaag atctctgaat tagttctcca aatattgcat
      181 caatctaatg tttctcaaat aaaaattaaa gagattgaaa aacgaattga tataattgtg
      241 tacaaaacat ttgagctaaa caataatgag attaaattga ttaacgaaag ctaatatatt
      301 attaaagagc attatggggc tgtgtcaaaa ttcaattttg ggattcacta acttacaatt
      361 tataagtaag ttattctcac aagccaaaaa taagccatat ttaccttgaa aacagggggg
      421 taaatatggc ttttccatta ttatagtatc aaactataac attttagaag gggaatttta
      481 gttttgacac agccccataa tttatatatt catataactg tctttgttta tctcaaattt
      541 tgggtctata gttagcactt ctttataagt taaaccgtat agatgatata ctagaatatc
      601 tatttctttt tgttcttttt caatatttgc ttgcggatct ttagatttta ttgaagtaat
      661 tctgtcaact tcttgtgata ttttaaattc aaggttacaa ttataatcaa cggcattttt
      721 atcactaagc aatggtaatg gaagttgttt tagatgcaat ccttttatat acggaaagac
      781 ttcagcgttt gttattaatt ttttattaaa atagtaattt aataatttag aattaaggat
      841 tgcacagcaa actttattat tataattatc cgtctttaaa tataagttat aaatactttg
      901 gtccgtataa tattgttcgt tatctataac agctaataat acattgcctg tcttgcggac
      961 aagtattttt tctgactgtt cgtatactga cgtgtcagta ttactatgta ataaattcgg
     1021 ttgaaaattt atataaatcg aattgtattt tattatatat ggtgctatat gtttaccttg
     1081 taaatatttt ttaaaactat cagtttcttt tgttttatat aaaaactttt ttataccatt
     1141 tgccactata ccgacataaa cattataaat atctcctaat tgtaacccca aagattccat
     1201 tttaaaaaat atattacaat ccgtagcgtt ggtctttaca tcaataacaa atgaagtttt
     1261 aatattattc catatctctg ttttgataaa ctgttggtca acgtctttaa aacttaaatc
     1321 cttaactctt ttataggaca taatgttgtt atttttatat ccattgttgc atcttaatat
     1381 gagtgagtct gttacagcat caaagattct attgtcaaac attacaattt cctttatttg
     1441 attttctgta agcaaaaagc gtctaatttt ttttagataa ctattttgta gaatagagcc
     1501 aggtataata tatgacagat taccatctgt tcgacaaaga gtgagacctt cttctataaa
     1561 caaagcatag ctattaactt tgtattctgc agatttttta taatgtgtaa ttaaaatttt
     1621 taattcatca tctttaattg atgttttttc ctttcctcta tatgttatat atggagggtt
     1681 cccgataaca atatcaaatc cgttgcaacc attcggtcaa ctgattgcca tatttccatc
     1741 agtcctttgt aaaaatctaa atatagtgga gggattaaca tgtaattttc gagcaatttt
     1801 agctttggac atgccatcgt taatccactt gtgtatatag tcttctttac cagtcaactt
     1861 agtgtaacta tttttgctgc ctaattttct tcctagcaca actcccttgg ctttcttcct
     1921 agctaatgcc tcttttgttc tttggctaat caaatttctc tctatttcag cagacaaccc
     1981 gaaagcaaac gccaatactt tactttggat atcttcgcct aagcgatagt tatctttgat
//
LOCUS       DAOMTA010000601         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2727_length_2039_cov_2.748488, whole genome shotgun sequence.
ACCESSION   DAOMTA010000601 DAOMTA010000000
VERSION     DAOMTA010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_2039_cov_2.748488"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLK68_13415"
     CDS             <1..873
                     /locus_tag="PLK68_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRM91444.1"
                     /translation="FKKKSEFQYTPRYGGKSIVPSPGDIIFFCKGNKNDSTHTGIVID
                     VTDNTVHTIEGNSSNTIKRNSYKTDSKSILGYGIPAFPNSISNGVSGMLSEEFKYFAK
                     YESSCNYDQGFSSSDDYHALGYYQFDNRYDLQKFLKYCYQSDNNKYACFEAYLSCSKA
                     SLRNNKGLEKAWHTAYSNDSKDFANKQDQFEYDNYYIPIENALKSLGIDLSSRRDCIK
                     GLVCGMANLFGNSGAKRLIKNSGVTNDLGDKEFVTKLCDYLVSYCPKHYAYGKSYANR
                     YKNEKNDCLRYISN"
     gene            929..1525
                     /locus_tag="PLK68_13420"
     CDS             929..1525
                     /locus_tag="PLK68_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91445.1"
                     /translation="MMCLLENKVEEYYKLNGEYMVYSDMLEYGFTKREIRRLVEKELL
                     RKLVKGLYIYKNELEDEFFVYQFANKNMIYSHETACYLHGLTTVIPSRCNVTTYSGCH
                     LRNNRLKVSYVKKELLHVGAVEHIDYFGNKIIVYDCERTICDLIRNKKKVDTQVYYQS
                     LQSYFNNKKLDMRKLSKYGKLFNVESEIAEIYSLYKSA"
     gene            1536..>2039
                     /locus_tag="PLK68_13425"
     CDS             1536..>2039
                     /locus_tag="PLK68_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRM91446.1"
                     /translation="MAYKNIDQWKNGMIKYAKEKNMDLRDVQQRFILEEFAEKISNSK
                     YRDSLIIKGGFVVSNLLGFENRTTLDMDATVNSTVYSVEEINNMITDIIEEDITKSFF
                     DYRIGDIKEGQSDDGYPGYTVSILALKGKTRLNLKIDISNNTLIYPEAIEHTFNSLFS
                     DKKINVCT"
BASE COUNT          790 a          229 c          382 g          638 t
ORIGIN      
        1 ttcaagaaaa aaagtgaatt tcaatatacc cctagatatg ggggaaaaag tatagtccct
       61 tcgccaggag atattatttt tttctgtaaa ggaaataaaa acgattcaac gcatacagga
      121 attgtaatag atgttacaga taatactgtc catacaatcg aaggtaactc gagcaataca
      181 ataaagcgta attcttataa aacggacagc aagtccattt taggatacgg aattcctgca
      241 tttccaaatt caatttcaaa cggagttagc ggaatgcttt ctgaggaatt taaatacttt
      301 gcaaaatatg aaagtagctg taattatgat cagggattta gcagttctga tgactatcat
      361 gcactgggat attaccagtt tgataacaga tacgatcttc aaaaattctt aaaatactgt
      421 tatcaatcag ataataataa atacgcttgt tttgaggcat atttaagctg ttctaaagca
      481 tctttaagaa ataataaggg attagaaaaa gcttggcata cagcttattc taatgattca
      541 aaagattttg cgaataagca ggatcaattt gaatacgata attactatat accgatagaa
      601 aatgcattaa aatcattagg aatagattta tcgtcgagaa gagattgcat taaaggcctt
      661 gtctgtggta tggctaatct ttttggaaat tctggtgcta aaaggcttat aaaaaattcc
      721 ggtgttacta atgatctggg cgacaaagag tttgtaacta agctgtgtga ttatcttgtg
      781 tcatattgtc ctaaacatta tgcatatgga aaatcatatg caaacaggta taaaaatgaa
      841 aaaaacgact gtttaaggta tatttctaat taatatgtga atattaaaca tatatatgtt
      901 ataatatatg tacgatagga acggtgatat gatgtgttta ttagaaaata aagttgaaga
      961 gtactataaa ttaaatggtg aatacatggt ttatagtgat atgctggagt atggatttac
     1021 taaaagagag ataagacggc tggttgaaaa agaactttta agaaaacttg ttaaagggct
     1081 gtatatttat aaaaacgaat tagaagatga attttttgtc tatcagtttg caaataaaaa
     1141 tatgatctat agtcacgaaa cagcgtgcta tttacatggg ctaacaacag taattccatc
     1201 cagatgcaat gtaacaacat attcaggatg ccatttaagg aataatcgtt taaaagtatc
     1261 gtatgtaaaa aaagaactgc tgcatgtagg ggcagttgaa catattgatt attttggtaa
     1321 taaaattatt gtctatgatt gtgaaagaac tatttgtgat ctgattagaa ataaaaagaa
     1381 agtggataca caggtatact atcagtcctt acagtcgtat tttaataata aaaaattaga
     1441 tatgagaaaa ttgagcaaat atggaaaatt atttaatgta gaaagtgaaa tcgcagaaat
     1501 ttatagtctg tataaaagtg catagaggag aaattatggc atataaaaat atagatcagt
     1561 ggaaaaacgg aatgataaaa tatgcaaaag aaaagaatat ggatttaaga gatgttcagc
     1621 agcgctttat tcttgaagaa tttgcagaga aaataagtaa ttctaagtac agggacagtc
     1681 tgattattaa gggaggtttt gtcgtcagca atttattagg ttttgaaaat agaaccacct
     1741 tagatatgga tgctacggta aattctacag tttattctgt agaggaaata aataatatga
     1801 taactgatat tatcgaagaa gatattacaa aatcattttt tgattataga ataggtgata
     1861 ttaaagaagg gcagtctgat gatggatatc caggctatac agtttctatt ttagcgctaa
     1921 aaggtaagac aaggttaaac ttgaaaattg atatttcaaa taatacatta atttatccag
     1981 aggcaataga gcatactttt aacagtttat ttagtgataa aaaaataaat gtatgtact
//
LOCUS       DAOMTA010000602         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2729_length_2038_cov_2.077156, whole genome shotgun sequence.
ACCESSION   DAOMTA010000602 DAOMTA010000000
VERSION     DAOMTA010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_2038_cov_2.077156"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /gene="smpB"
                     /locus_tag="PLK68_13430"
     CDS             complement(<1..278)
                     /gene="smpB"
                     /locus_tag="PLK68_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRM91447.1"
                     /translation="MKIVSNNKKAYHDYFILDTYEAGIELKGTEIKSVRLGNVNLKDA
                     FVRIKDNEAYIENMHISPYSHGNQFNHEPLRNRKLLLHKKEILKISNK"
     gene            complement(302..>1583)
                     /locus_tag="PLK68_13435"
     CDS             complement(302..>1583)
                     /locus_tag="PLK68_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HRM91448.1"
                     /translation="TISCFMEIDENGEVVEHDIVPAVINSTERMTYTNVNKILDGDQT
                     LQLQYSHVKDLFFLMQELAMILQAKKARRGAIDFDVKEAKVLVDSKGNTTDVVLRERG
                     ASDRIIEEFMLLANETVAEHFKWLELPFIYRVHENPKPKKLLQFSGIAKTLGYTIKGS
                     LENVYPGELSNIIEVSKDTPEHTIIATLLLRCMQKARYDEQCLGHFGLADEYYTHFTP
                     PIRRYPDLIVHRLIRKYMFENRLDRRTIMHYQELMPEIALQTSAREREAIMIEREVDD
                     MKIAEFMEKQIGEEFEGIISSVTNFGFFVELDNTIDGLVHVTDLTDDFYFFDEKNLRY
                     IGQRTGKVFKMSDRVKVRVSSASKKDKSVDFEIVGMKSNKKTTKTVIINKRKDNDRKN
                     NRKRNDRRKKRHEEPRFTNNHFKRSKKGKSKRRA"
     assembly_gap    1584..1606
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1607..>2038)
                     /locus_tag="PLK68_13440"
     CDS             complement(<1607..>2038)
                     /locus_tag="PLK68_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRM91449.1"
                     /translation="KPILKGDVVKILGHRNDPGIDILSIVYEHDAPVEFPQAVYDQIE
                     NIPDSLEGIDIGQRIDLRDEVVVTIDGDDAKDLDDGISLKKLDNGHYHLGVHIADVSY
                     YVTEESPLDKEAFARGTSIYLADRVIPMLPHKLSNGICSLNP"
BASE COUNT          645 a          401 c          262 g          707 t
ORIGIN      
        1 aatttatttg aaatttttaa aatttctttt ttatgaagta atagttttcg attacgtaac
       61 ggctcatgat taaattgatt accatggcta taaggagaaa tgtgcatatt ttcaatataa
      121 gcttcattat ctttaattct tacaaaagca tctttaagat ttacattacc taaacggacc
      181 gatttaattt ctgttccttt tagttcaatc cctgcttcat atgtatctaa gataaaataa
      241 tcatgataag ctttcttgtt atttgataca attttcatgc ccatttcccc tttctgatta
      301 attaagcgcg acgcttagat ttaccctttt tagagcgttt aaaatgatta tttgtaaaac
      361 gtggttcttc atgacgcttt ttacgacgat cattccgttt tcgattgttt ttacgatcat
      421 tatctttgcg cttatttatt ataacagttt ttgttgtctt tttgttggat ttcatgccaa
      481 caatttcaaa atctactgat ttatcttttt tagatgcact cgatactcga actttaactc
      541 gatcagacat cttaaacacc ttgcctgttc gctgaccaat ataacgaaga ttcttctcat
      601 caaagaaata aaaatcatca gtcaaatcag ttacatggac taacccatca attgtattat
      661 caagttcaac aaagaaacca aaattagtaa ctgaactaat aattccttca aattcctcac
      721 caatttgttt ttccataaac tccgcaattt tcatatcatc aacttcacgt tcaatcataa
      781 tcgcttcacg ttcacgagcc gaagtttgta gtgctatttc aggcatcaat tcttgataat
      841 gcatgatagt acgacgatct aaacgatttt cgaacatgta ctttctaatt aaacgatgaa
      901 cgattaaatc tggataacgt ctaattggtg gagtaaaatg tgtataatat tcatcagcta
      961 atccaaaatg tcctagacac tgttcatcat aacgcgcttt ttgcatacaa cgcaataata
     1021 aagttgcaat aattgtatgt tccggagtat ccttactaac ttctatgata ttgcttaatt
     1081 cacccggata aacattttct aatgaacctt tgattgtata gcctaaagtt ttagcaattc
     1141 cactaaattg taataacttt ttcggtttag gattttcatg aacccgataa atgaacggca
     1201 gctctaacca tttaaaatgc tctgcaactg tttcatttgc caataacata aattcttcaa
     1261 taatacgatc acttgcccca cgttctctca atacaacatc agtagtatta cctttactat
     1321 caactagtac ttttgcttct ttaacatcaa aatcaatcgc accacgtcta gcttttttcg
     1381 cttgaagaat cattgctaat tcctgcatta agaaaaataa atctttgaca tgactatact
     1441 gtaactgaag cgtctgatca ccatcaagaa ttttatttac attagtataa gtcatccgtt
     1501 ctgtcgaatt aattactgct ggaacaatat catgttctac aacctcacca ttttcatcaa
     1561 tttccataaa acaagaaatt gtgnnnnnnn nnnnnnnnnn nnnnnncgga tttaatgaac
     1621 aaatgccgtt tgataactta tgtggcagca ttgggataac acgatcagct aaataaattg
     1681 atgttccacg tgcaaatgct tctttatcaa gcggtgattc ttctgtaaca taataagaaa
     1741 catcggcaat atgcacccct aaatgataat gaccattatc taattttttt aatgaaatac
     1801 catcatctaa gtctttggcg tcatcaccat caatagtaac aacgacctca tcacgcaaat
     1861 caatccgttg accaatatca attccttcca aactatcagg aatattctct atttgatcat
     1921 agacagcttg aggaaactca actggcgcat cgtgttcata tactatcgat aagatatcaa
     1981 tcccaggatc attgcgatgc cctaaaatct ttacaacatc accttttaaa atcggttt
//
LOCUS       DAOMTA010000603         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2735_length_2036_cov_1.988894, whole genome shotgun sequence.
ACCESSION   DAOMTA010000603 DAOMTA010000000
VERSION     DAOMTA010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_2036_cov_1.988894"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..318
                     /locus_tag="PLK68_13445"
     CDS             13..318
                     /locus_tag="PLK68_13445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM91450.1"
                     /translation="MIRILLCCAGGMSTSLLVTKMEQAAQEKGIETKIWAVGATEVKN
                     HSQDADVILLGPQVRYLEKSIADDANGVPAYLIDMRDYGKMDGKAVLTFALNKLQEV"
     gene            321..1748
                     /gene="ascB"
                     /locus_tag="PLK68_13450"
     CDS             321..1748
                     /gene="ascB"
                     /locus_tag="PLK68_13450"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM91451.1"
                     /translation="MKMVEKNFPKGFLWGGAIAANQCEGAVLEDGKGYSTADALPEGV
                     FGNEAIPPLDNYLKKNAIDFYHRYKEDIALFAQMGFKVLRLSIAWSRIFPHGDETVPN
                     EKGLQFYDDVFDELAKYGIQPLVTLSHYEMPLYLATNYGGWVNRKVIDFYLNFAQVCF
                     ERYCNKVKYWLTFNEINVILHAPFNGGGIKGKADQINPEVLYQAIHHQFVASAQATKI
                     AHKINPNFKIGCMIAGSPIYPLTPQPEDIQETVRRDRESLFFADVHVRGKYPGYMLRF
                     FEENHIHLEITKEDEEILKNTVDFISFSYYMSYCATADEKLNQQARGNVMSGVKNPYL
                     KESEWGWQIDPKGLRYILNQFYDRYQLPLFIVENGLGAKDILVVDEQYGYTVNDDYRI
                     DYLKEHLKQVLEAIKDGVEVLGYTSWGPIDIVANTKCQVSKRYGYIYVDVHDDFSGTL
                     ARYPKKSFWWYQKVIATNGESLNDI"
     gene            1875..>2036
                     /locus_tag="PLK68_13455"
     CDS             1875..>2036
                     /locus_tag="PLK68_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM91452.1"
                     /translation="MILEKLRERKRFTNSEKLIAEYVLKHPEQLYQLSVEELGKETYT
                     SKATVIRLCK"
BASE COUNT          694 a          262 c          439 g          641 t
ORIGIN      
        1 aggaaaatta aaatgataag aattttatta tgttgtgctg gtggaatgtc aactagtcta
       61 ttagttacaa aaatggaaca agcagcacag gaaaaaggaa ttgaaacaaa aatctgggca
      121 gtcggagcaa cagaagtaaa aaatcacagc caagatgcag atgttattct tttaggacca
      181 caggtgcgtt atctagaaaa aagtattgcg gatgatgcta atggagtacc agcatattta
      241 atagatatgc gcgattatgg caagatggat ggaaaagcgg tattgacttt tgcactaaat
      301 aaattgcaag aggtgtagtc atgaagatgg tagaaaaaaa tttccctaaa ggatttttat
      361 ggggtggtgc aatagcggct aatcagtgtg aaggcgcagt cttagaagat ggcaaaggtt
      421 attcaacggc tgatgctttg cctgaagggg tatttggtaa tgaagcaatt ccgccattag
      481 ataattattt aaagaaaaat gcaattgact tttatcatcg ctataaagaa gatattgcat
      541 tgttcgctca gatgggattt aaagtattac gcttatcgat agcatggagc cgtattttcc
      601 cgcatggtga tgagacggtt cctaatgaaa agggtttaca gttttacgat gatgtttttg
      661 atgaattagc taaatatgga atacaacccc tagttacatt atcacattac gagatgcctt
      721 tatatctagc gactaattat ggaggatggg tgaatcgtaa agtaattgat ttttatctta
      781 actttgctca agtatgtttt gaacgttatt gtaataaggt taagtactgg ctaacattca
      841 atgaaataaa tgttattctt catgcccctt ttaatggtgg tgggattaaa ggaaaagctg
      901 atcaaattaa tcccgaagtt ttatatcagg caattcatca ccagtttgtt gcaagtgctc
      961 aagcaacaaa aatagctcat aaaatcaatc cgaattttaa aattggttgt atgattgcag
     1021 ggagtccaat ctatccattg accccgcagc ctgaagatat tcaggaaaca gtccgacgtg
     1081 atcgggagtc actattcttt gctgacgtcc atgttagggg aaaatatcca ggatacatgc
     1141 tacgtttttt tgaagaaaat catattcatt tagaaatcac gaaagaagat gaagaaattt
     1201 taaaaaatac agtcgatttt atttctttta gttattatat gagttattgt gctacagctg
     1261 atgagaaact taatcaacaa gctcggggaa atgtaatgag cggagttaaa aatccctatt
     1321 taaaagaatc tgaatggggt tggcagattg atcctaaagg gttgcgatat attcttaatc
     1381 aattttatga tcgatatcag cttccgttat ttattgttga aaatggtctt ggagcaaagg
     1441 atattttggt tgtagatgaa caatatggtt atacagtaaa tgatgattat cgtattgatt
     1501 atttaaaaga gcatttaaag caggttttag aagcaatcaa agatggtgta gaagtactcg
     1561 gttatacaag ttggggtcca atcgatattg ttgctaatac aaaatgtcaa gtatctaaac
     1621 gttatggcta tatttatgtt gatgtgcatg atgattttag tggaacgttg gcgcgttatc
     1681 ccaagaagag tttttggtgg tatcaaaaag ttattgcaac gaacggtgaa agtttaaatg
     1741 atatttagct gttttgtaaa atggaggagg attttcaatc tcctttattt taattaataa
     1801 aaagataatt gattagttca aatttgtttc atcttgatat ctatgtcata ataggaaaag
     1861 atgaggtgat agtgatgatc ttagagaagc taagggagag gaaacgcttt actaatagcg
     1921 aaaagctaat tgcagagtat gttttaaaac atcctgaaca gttatatcaa ttatcagtgg
     1981 aagaattagg aaaagaaact tatacgagta aggctacagt aatacgtctt tgtaaa
//
LOCUS       DAOMTA010000604         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2737_length_2034_cov_2.034866, whole genome shotgun sequence.
ACCESSION   DAOMTA010000604 DAOMTA010000000
VERSION     DAOMTA010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2737_length_2034_cov_2.034866"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLK68_13460"
     CDS             complement(<1..297)
                     /locus_tag="PLK68_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM91453.1"
                     /translation="MRKQVTYLGNILCYEIENLNKPVCICFHGMNMTREMYKTDQLQH
                     LLKNYSLVLVDLCGYGDSSIETKNFNMVVFNQLVLKLVKCENIKSCTIVGYCLGG"
     gene            complement(294..683)
                     /locus_tag="PLK68_13465"
     CDS             complement(294..683)
                     /locus_tag="PLK68_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91454.1"
                     /translation="MEPLPCGALKNKLEEVLTDNEKEQLKQLHITLNRDSRYPKASVF
                     PYFESAEQFGCGAGVQHSYIDGHGNFGPCDFIEPTFGNVLNENIQYIWVKMSKAINGP
                     HCQCIAKNCDICKQLPRFYKLMRGYKK"
     gene            complement(713..1501)
                     /locus_tag="PLK68_13470"
     CDS             complement(713..1501)
                     /locus_tag="PLK68_13470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM91455.1"
                     /translation="MNKRMIIKMCINPHIIKTFLNLHCQFRKHEDYKVRNMVKCGLNI
                     IINDHLVQHDGYYLVNSFIPPINTEAFRTVVKMVPGKGKNFFVNHTEGIRLAPISTYI
                     AVTDKCMYHCWHCSASKFMKDATSNSQFSTLELKKIIAQLQRLGVAIIGFTGGEPLLR
                     EDLEELIASIDERSVSYIFTSGYKLTYQRALALKEAGLFGIAISLDSLDAQRHNGMRH
                     NDHAYDYAVEAIKNAKKAGLYTMSQTVCTRELLINGGILKLALY"
     gene            complement(1507..>2034)
                     /locus_tag="PLK68_13475"
     CDS             complement(1507..>2034)
                     /locus_tag="PLK68_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM91456.1"
                     /translation="KVCKGMLEFLTVQESCCYDAIVCTWALMYEQPQKVLNQCQRLLK
                     NGGYLYILVNDQQTLPQIRKIYPKLLIEYVGSINKLMFDLPMPNNAQQLERWAKKAGL
                     KNCRVTSKKQEFYFTDWNRAAKFVTSTGALAGYDAMLDLKNEKIFNTLVEQLQKFFTK
                     PCITHHFVMGIFKKG"
BASE COUNT          664 a          392 c          255 g          723 t
ORIGIN      
        1 accccctaaa caatatccaa caatagtaca agatttgata ttttcgcact tcactaattt
       61 taataccagc tgattaaata ctaccatatt aaaattcttt gtttctatcg aagaatcccc
      121 atatccgcat aaatcaacta aaaccaaaga ataatttttt aataaatgtt gcagttgatc
      181 tgttttatac atttctctag tcatattcat tccatgaaaa caaatacaga ctggtttatt
      241 taaattttca atttcatagc aaagaatatt tcctaaataa gtcacttgct ttctcatttt
      301 ttataacctc gcattagttt ataaaatcga ggtaattgtt tacaaatatc acaattttta
      361 gcaatacatt gacaatgcgg gccatttatt gcttttgaca tttttaccca aatatattga
      421 atattttcat tcaacacatt tccaaatgtc ggctcaataa agtcacaggg accaaaatta
      481 ccatgtccat caatatatga atgctgtact ccagctccac agccaaattg ttctgcactt
      541 tcaaaatatg gaaagacact ggctttagga tatcgagaat ctctatttaa tgtaatatgc
      601 aattgcttta attgttcttt ttcgttatca gttagaactt cttccaattt gttttttaaa
      661 gcaccacaag gtaagggctc catgattcgc atttcatcta tttctaattt ttttaataca
      721 gtgccagctt taaaattcct ccattaatca gtaattctcg tgtacatacc gtttgactca
      781 ttgtataaag ccctgccttt ttagcatttt taatagcttc aacagcataa tcgtaagcat
      841 gatcattatg gcgcattcca ttatgtcttt gggcatctaa agaatcgaga cttattgcaa
      901 taccaaataa ccccgcttct tttaaagcca gagcacgttg ataagttaat ttatatccgc
      961 ttgtaaaaat ataactaaca ctacgttcat caatacttgc aataagctcc tctaaatctt
     1021 cacgcaataa aggttcacca cctgtaaaac caataattgc tacacctaat ctttgtaatt
     1081 gggcaatgat tttttttaat tctaaagtcg aaaattgact atttgatgtg gcatctttca
     1141 taaacttaga tgcactgcaa tgccagcaat ggtacataca tttatctgta acagcaatat
     1201 atgtagaaat cggagccagc cgaatccctt cagtgtgatt aacaaaaaaa ttttttcctt
     1261 taccaggaac catcttaact actgtcctaa aagcttcagt attaatcggt ggaataaatg
     1321 aattcactaa ataataacca tcatgctgga ctaaatgatc atttataatt atatttaatc
     1381 cacattttac catatttcta accttataat cttcatgctt gcgaaattgg caatgcaaat
     1441 ttaaaaatgt tttaataatg tgaggattga tacacatttt aataatcatc cgtttgttca
     1501 ctcaggtcac cccttcttaa atattcccat cacgaaatga tgagtaatgc atggtttagt
     1561 aaaaaacttc tgcagctgct caacaagagt attaaaaatt ttttcatttt tcaaatccaa
     1621 cattgcatca taaccagcca aagcacctgt tgaggtaaca aattttgcgg cccgattcca
     1681 atctgtgaaa taaaattctt gtttttttga agttactcga caatttttta atcctgcttt
     1741 tttagcccaa cgttccaatt gctgcgcatt gtttggcatt ggtaaatcaa acattaattt
     1801 attaattgag cctacatatt caattaataa ttttggataa atttttcgaa tttgtggcaa
     1861 tgtttgctga tcatttacta agatatacaa atatccccca ttttttaata accgctgaca
     1921 ttgatttaat actttttgtg gttgttcgta catcaatgcc caagtacata cgattgcatc
     1981 atagcaacaa ctttcttgta ctgttaaaaa ttcaagcatt cctttacaca cttt
//
LOCUS       DAOMTA010000605         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2738_length_2034_cov_1.481051, whole genome shotgun sequence.
ACCESSION   DAOMTA010000605 DAOMTA010000000
VERSION     DAOMTA010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2738_length_2034_cov_1.481051"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1533)
                     /locus_tag="PLK68_13480"
     CDS             complement(196..1533)
                     /locus_tag="PLK68_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91457.1"
                     /translation="MNERLANEKISKLLLSLAIPSILAQMVTLLYNLVDRIYIGRMED
                     GALAIAGIGLCSAIITIITAFTNLFGRGGAPLASISLGADENDQANKIISNCFSSLVL
                     SSIVIMIVLYIFGEEILLLFGASANTLSYAKDYLNIYLLGTIFVQLSVGMNYFINCQG
                     YAKFGMLTLVIGGALNIILDPIFIFTLNMGVAGAALATIISQFVSFLWVMHFIFSKRS
                     TIKIKKEYLFFDKIIMKRVLGLGISPFFMNSTEGILQVCFNRQLLFFGGDIAVSSMTI
                     MASMAQIVFLPMEGIAQGSQPIISFNYGARQKERVIEAIKMVIKVALTFSVIMVTLME
                     LFPALFVSMFTNDPELMELGVKMLRVYIFGYIIIGANSSFQQIYTSLGEGKRSFFFAF
                     YRKIILLIPLIYILPNFISNGVLAVMLAEPVSDLLTTGTNAIFFKRFINDKLK"
     gene            complement(1526..1960)
                     /locus_tag="PLK68_13485"
     CDS             complement(1526..1960)
                     /locus_tag="PLK68_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRM91458.1"
                     /translation="MKKTFLMAIYPYLLKQYNIMFETIQKKYQITQIEIDVLAFLANN
                     PEYHHAQDIVNIRGISKAYVSCSLDKLVKRGFVERQVDNENRRCNCLFVTPYANELIK
                     EIRAVQDNYNEIAYQGLSNQDKEQFNRLISQIYENLGGNNNE"
BASE COUNT          701 a          392 c          248 g          693 t
ORIGIN      
        1 cgctaattct tattttaaat caagtacaag ttattccacc ttttaattga ctctttatat
       61 attctttgta acttttccta ttataactac ataattttct ctgcactaaa gctttttctt
      121 gacccataga ttatctgtga attttattgc atacaaaaag cgacttatcc tgtcgccttt
      181 cttattttta tgtaattatt tcaacttatc attgataaaa cgcttgaaga aaatcgcgtt
      241 agttcctgtt gttaataaat cagatactgg ttctgccagc attaccgcta atacaccatt
      301 gctaatgaaa ttaggtaaaa tataaatcaa aggaattaat aaaattatct tgcgatagaa
      361 agcaaagaaa aatgatcttt ttccttcgcc caatgatgta tagatttgtt gaaaagagct
      421 attagcacca ataataatat agccaaagat atacactctt aacatcttta cccccaattc
      481 cattagttct ggatcgttag taaacatgct aacaaaaagc gccgggaaca actccatcaa
      541 agttaccatt attactgaaa atgtcaaggc caccttaatt accattttga ttgcctcaat
      601 aactcgttct ttttgtctag caccataatt aaaagaaatt atcggctggc tcccttgggc
      661 tattccttcc attggtaaaa agacaatttg ggccattgaa gccataattg tcatagaact
      721 aacggcaata tcaccgccaa aaaataataa ttgtcgatta aaacatactt gtaagattcc
      781 ctccgtacta ttcataaaaa atggtgaaat ccctaaaccc aatactcttt tcataattat
      841 tttatcaaag aacaagtatt ctttctttat ttttattgtt gaccgtttcg aaaaaataaa
      901 atgcattacc cataaaaaag aaacaaattg cgaaatgatc gtagccaagg ccgctcccgc
      961 tactcccata tttaaagtaa aaataaaaat tggatctaga ataatattta acgcgcctcc
     1021 aataactaac gttaacattc caaacttcgc atacccttgg caattaataa aataattcat
     1081 tcccacactt aattgtacaa aaatcgtccc caataaataa atatttaaat aatctttggc
     1141 atatgataac gtatttgcac ttgcaccaaa caataataaa atttcttcac caaatatata
     1201 taaaacaatc atgataacta ttgaacttaa tactaaagaa ctaaaacaat ttgagattat
     1261 tttatttgct tggtcatttt catcagctcc tagacttatt gaagctaatg gtgccccacc
     1321 acggccaaac aaattagtaa aagcggtaat tattgtaata attgcactac acaaaccaat
     1381 tccagcaatt gctaaagctc catcctccat tcttccaata taaattcgat ccactaaatt
     1441 gtatagcaag gttaccattt gtgctaagat cgatggtatc gccagtgata acagtaactt
     1501 tgaaattttt tcattagcaa gtcgctcatt cattgttatt ccctcctaaa ttttcataaa
     1561 tttggctaat taatcggtta aactgttctt tatcttgatt acttaacccc tgataagcaa
     1621 tttcattata attatcttga acagcccgaa tttctttaat cagctcatta gcatatggtg
     1681 ttacaaacaa acaattacaa cgacgatttt cattatctac ttggcgttca acgaaccctc
     1741 ttttgactag tttgtcaagc gaacaagaaa catacgcctt agaaatacca cggatattga
     1801 caatatcttg agcatgatga tactcaggat tattagctaa aaatgccaat acatcaattt
     1861 caatttgagt gatttgatac tttttttgta ttgtttcaaa cataatatta tattgcttta
     1921 ataaataagg ataaattgcc attaaaaagg ttttcttcat ttctcttcat tctttattct
     1981 ctattcttgt ctgcctgttt tatttcaaaa agagtcttca agctgtgaga ttct
//
LOCUS       DAOMTA010000606         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2742_length_2031_cov_1.969130, whole genome shotgun sequence.
ACCESSION   DAOMTA010000606 DAOMTA010000000
VERSION     DAOMTA010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_2031_cov_1.969130"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2031)
                     /locus_tag="PLK68_13490"
     CDS             complement(<1..>2031)
                     /locus_tag="PLK68_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRM91459.1"
                     /translation="EALASSPELLGQRIDALYQLTTDQPKILITHGQALVRYLPSRQL
                     FLDNCLNLKTGMQIDIYDLQKLLIKAGYTSAPRVDQPFYFSKRGGVIDVFSIQYDNPL
                     RIEFFDDEIDNIRFYNQNSQRTIEKVKEVTIIPASDILYDEQEVPAVLSKINDLRDRQ
                     IEELDELYQEDYLSKVSIDQENLRNHDTSFTMYGYFNLFNQTASLLDYLDTPLIIQAN
                     NHDINFAYKNYLEENHYYYQELASIGKTIKGLNLFRDLYEVIDRKSVNFKPFAQSDKD
                     VLFNARAIMINNDDEAMIINQIRAYLKLSKVVVALDDDHQLKLMTELFDRHEMAYTLI
                     GIKDEIYPGLNIAVNKIGFGIELVDEKIVIISANELFKTRNIKKPKYFKYKNAKVLKD
                     YQELNIGDYVVHDNHGIGQYLGIKTLEVQGFHKDYLYVAYAGDDTLYIPVEQFKMIRK
                     YSSNEGKVPKINKLGGSQWQKTKAKARSKVDDIADKLIEIYSARINQPGYAFPSDSEI
                     QLEFERSFGYELTVDQLRSVEEIKADMEKSQPMDRLLCGDVGFGKTEVALRAAFKAIL
                     GNKQVAFLCPTTILSMQHYKTMIARFKDFPVKIALLNRFTSTKEKKQILSDLKLGNID
                     LLVGTHRILSKDIVFKDIGLLCIDEEQRFGVKQKEKIKEIRKTIDVLTLTATP"
BASE COUNT          668 a          382 c          240 g          741 t
ORIGIN      
        1 ggtgtagcag ttaaagttaa aacatctatt gtctttctga tttctttaat tttctctttt
       61 tgtttaacac caaaacgctg ttcttcatct atacataata atccaatatc tttaaaaaca
      121 atatcctttg ataagattcg atgtgtccct acaagcagat caatatttcc taattttaaa
      181 tcacttagta tctgcttctt ttccttagtc gatgtaaagc gattcaataa agcaattttg
      241 acaggaaaat ccttaaatcg tgcaatcatt gttttataat gctgcattga taaaattgtt
      301 gtgggacaca aaaaagcaac ctgtttattt cctagaatag ccttaaatgc tgctcttaaa
      361 gcaacttccg ttttcccaaa tccaacatct ccgcatagca agcgatccat cggctgactc
      421 ttttccatgt cggctttaat ttcttcaact gaacgtaact ggtcaactgt taattcataa
      481 ccaaatgagc gttcaaactc cagctgaatc tcactatcac ttgggaaagc atagccaggc
      541 tgattaatac gagcagaata aatttcaatt aacttatcag caatatcatc aactttactc
      601 cgtgctttgg cttttgtttt ctgccattgt gagcctccca atttattaat tttaggaact
      661 ttaccctcat tacttgaata cttcctaatc atcttaaatt gttcaaccgg tatatataaa
      721 gtatcgtctc cagcataggc tacatataaa taatctttat gaaatccttg aacttctaat
      781 gttttaattc ctaagtattg ccctatccca tgattatcat gtacaacata atcaccaata
      841 tttaattctt gataatcttt taaaaccttt gcattcttat acttaaaata ttttggtttt
      901 ttaatattac gtgttttgaa taattcattt gcacttataa taacaatttt ttcatcaact
      961 aattcgattc caaaaccaat tttatttacc gcaatattca accccggata tatttcatct
     1021 tttatcccaa tcaaagtata tgccatttca tgacgatcaa ataattcagt cattaatttt
     1081 aattgatgat catcatctaa agctacaaca acttttgata actttaaata agctctaatt
     1141 tgattaataa tcattgcctc atcatcatta ttgatcataa tagcacgagc attaaacaac
     1201 acatctttat cactttgagc aaaaggttta aaattaactg attttctatc aatcacttca
     1261 tataaatctc taaatagatt caaaccttta atagttttcc caatactagc caattcttga
     1321 taataataat gattttcttc taaatagttc ttataagcaa aattaatatc atgattgttt
     1381 gcttggatga ttagcggggt atcgagataa tctaataaac tagctgtctg attaaataaa
     1441 ttaaaataac catacatcgt aaaactagta tcatgattcc gcaaattttc ttgatctatt
     1501 gatactttac ttaaataatc ttcttgatat aactcatcaa gttcttcaat ctgccggtct
     1561 cttaaatcat tgattttact taaaacagcc gggacttctt gttcatcata taaaatatca
     1621 ctagctggaa taatggttac ttctttaacc ttttctatcg ttctttgact attttgatta
     1681 taaaaacgta tattatcaat ctcatcatca aaaaattcaa tccgtaatgg attatcatat
     1741 tgaattgaaa aaacatcgat tactccacca cgtttagaaa aataaaacgg ctgatctact
     1801 cgcggtgctg atgtataacc agccttgatc aaaagttttt gcaaatcata tatatcaatt
     1861 tgcattcctg tttttagatt taaacaatta tctaaaaata actgtctact tggtaaatac
     1921 cgtaccaggg cctgtccatg agtaattaaa attttaggct gatcagttgt caattgatat
     1981 aaagcatcaa ttcgctgtcc taatagttct ggcgatgagg ctaaagcttc a
//
LOCUS       DAOMTA010000607         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2765_length_2020_cov_1.900254, whole genome shotgun sequence.
ACCESSION   DAOMTA010000607 DAOMTA010000000
VERSION     DAOMTA010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2765_length_2020_cov_1.900254"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..440)
                     /locus_tag="PLK68_13495"
     CDS             complement(18..440)
                     /locus_tag="PLK68_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91460.1"
                     /translation="METKITLDSLIKEQENRINQCPLNEDPRYISTVYIYDDMLSYHN
                     WLAKTKRFLKTKFSNDKDIEEFDRISKTALESEQQLQLLAILEAFVDFPEPIIEKNVN
                     NKGSKSSNIISINNSNSQSQNQAIAINIFLEAIKPKFK"
     gene            complement(730..>2020)
                     /locus_tag="PLK68_13500"
     CDS             complement(730..>2020)
                     /locus_tag="PLK68_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91461.1"
                     /translation="KKAVNGKTRQFYLLAPGLQEYKIRRLAETNVKYYDAKAEDLFPN
                     IFSSLDQNICSDYKKQKISFTTFSKYSKNKGLNAIASEGKNCNDVNFSPIGASKVQLN
                     MVMDKNLRDKIMTNDASSYTEFVPDTSIPSLKIGKEELRNIEITINGITVCNKEDISF
                     VYLSPVSEQREVSFRILETGFNEKASITQFEFNNCVTISSKFECFTLTFKFDFNENAP
                     VICSCTFELTDKYKNNSEALKWIDLPIALFENKEIIISSIQNITIKTNQSNIPNELKN
                     IRKYYKNIKEIELLYYVGFKEYTNYSIEALKKTEMLIHSYYETPMYCPNEDKEQFINI
                     DGDIQSEDKQELLKNTPTGIALSYPVENIDFNGLSFKLKEKHIIIPSCDILYIKKKNK
                     NNTQIKINKHTNFLCLIYTNRDINEIDKYKNFKHLKS"
BASE COUNT          613 a          263 c          273 g          871 t
ORIGIN      
        1 cgaagcagtt gcattattca cttgaattta ggttttatgg cttctaaaaa aatatttatt
       61 gctatagctt gattttgcga ttggctattg gagttattaa tagaaattat attagaactt
      121 ttactacctt tgttgtttac atttttttcg atgattggtt ctggaaaatc tacaaatgct
      181 tccagaatag ctaataattg tagttgttgt tctgattcta atgccgtttt actaatccga
      241 tcaaactctt caatatcttt gtcatttgaa aatttagttt ttaaaaaccg tttagtttta
      301 gctaaccaat tgtgatatga tagcatatca tcatatatat aaactgttga tatatagcga
      361 gggtcttcat ttagcggaca ctgattaatg cgattttctt gttctttgat taaagaatca
      421 agcgtaattt ttgtttccat gattaaatat attgttgttg aatttataaa tgtttatgta
      481 atttttaatt aaaaatagtc gttaatatcc atgatgatat atagacttaa tcccagaatt
      541 gagtgttttt tctcgtttct ctttagaaat ctcttgaagg aggtcttata cgtagtatca
      601 attcttctat atagtgattc cattattcta ttgtattcat ttatccattt tttctttttc
      661 taattttagt aattctattt attttcttta caagaaaaac cacttcttta aattataaag
      721 catattggat taacttttta aatgtttaaa gtttttgtat ttatctattt cattaatatc
      781 cctatttgta taaattagac aaagaaaatt agtatgtttg ttaattttta tttgtgtatt
      841 atttttattt tttttcttaa tataaagtat atcgcaagat ggtattataa tatgtttctc
      901 cttcaattta aatgataatc cattaaaatc tatgttttct actggataag aaagtgcaat
      961 accagttggt gtatttttta ataattcttg tttatcttca gattgaatgt caccatctat
     1021 gttaatgaat tgctctttat cttcatttgg acaatacatt ggtgtttcat aataagaatg
     1081 aataagcatt tctgtttttt ttaatgcttc tatgctataa ttagtatatt ctttaaaccc
     1141 tacatagtat aacagttcaa tttcttttat gtttttgtaa tatttcctta tgttttttaa
     1201 ttcattaggg atattacttt gattagtttt tatcgttata ttttgtatag aagaaattat
     1261 tatctcctta ttctcaaata atgcgattgg taagtcaatc cattttagag cttcagaatt
     1321 atttttatat ttatcagtta attcaaaagt acaagaacat atgacagggg cgttctcatt
     1381 aaaatcaaat ttgaacgtta aagtaaaaca ttcgaactta gacgaaattg ttacacaatt
     1441 attaaattca aattgcgtga ttgatgcttt ttcattgaag cctgtctcta atattcgaaa
     1501 tgaaacttcc ctttgttcag atacgggtga taaatataca aaagaaatat cctctttatt
     1561 gcagacggta ataccattaa tagttatttc tatatttctg agctcttctt tgcctatttt
     1621 taatgatgga attgatgtgt caggaacaaa ttctgtatat gatgaagcat cattagtcat
     1681 tatcttatcg cgtagatttt tatccattac catattgagt tgaactttac ttgctcctat
     1741 tggagaaaaa ttgacatcat tacaattttt accttctgat gcaatagcat tgagtccttt
     1801 attcttacta tattttgaaa atgtggtaaa agatattttc tgtttcttat aatcactaca
     1861 tatattttgg tcaagtgacg aaaatatatt agggaataga tcttcagctt ttgcatcgta
     1921 gtatttgacg tttgtttctg ctagccttct tattttatat tcttgcaaac ctggagctaa
     1981 aagataaaat tgtctagtct taccatttac ggcttttttt
//
LOCUS       DAOMTA010000608         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2771_length_2017_cov_1.761978, whole genome shotgun sequence.
ACCESSION   DAOMTA010000608 DAOMTA010000000
VERSION     DAOMTA010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_2017_cov_1.761978"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLK68_13505"
     CDS             <1..1263
                     /locus_tag="PLK68_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM91462.1"
                     /translation="NTLKISGLNGGYGTKGWEAVVKAFEKKEGVKVELNLEKNIAETL
                     RPVITSGKDVPDLIYLSVGSEGGLVDTMISDKAIAEISDVLDMKVYGEDVKVKDKIIP
                     GFTNSFATKPYGDGKLYLAPFTYDPCGLFYNAGLFEQKGWTVPTTWDEMWELGEKAKA
                     EGIALFTYPTTGYFDAFFSALLNETVGADAYTKLMEYDTKTWQSAETKKAFEIVGKLA
                     QYTEANTVANANKDNFTKNQQLILDNKALFCPNGTWLPDEMSEAPRAEGFKWGFMALP
                     KVSAEGDSYSSTFSEQVYIPSKAKNADLAKKFITFMYSDEAAKLFAEESGCVLPTTTA
                     SSYLPEKVKDKDGNEIDNQKTLFYQIYDNGAKSCTVGFKSVDAIEGVDLTSAEGILYG
                     TVNSVVTGDKTVDEWYNAVIDAVKKYDK"
     gene            1325..>2017
                     /locus_tag="PLK68_13510"
     CDS             1325..>2017
                     /locus_tag="PLK68_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM91463.1"
                     /translation="MNKKKERRRFIILCLAPAVILFGVFMILPTLNVFWMSTLKWGGL
                     SADKTFVGFNNFVLLMQDMNFIRALQNTILIIAVVTVITMAVAILFASILVREKIKGQ
                     NFFRVIFYIPNILSIVVISSIFSAIYDPSENGMINSVLMLFKPESWETVKFLGDQSIV
                     IYAIIGTMIWQAIGYYMVMYMASMSSIPEHLYEASALDGAGKIKQFFSITLPLIWDNI
                     RTTLTFFIISTIN"
BASE COUNT          693 a          266 c          388 g          670 t
ORIGIN      
        1 aatactttaa aaatttcggg actaaatggt ggttatggga caaaaggatg ggaagctgta
       61 gttaaggctt ttgaaaaaaa agaaggggtt aaagttgaat taaatttaga aaaaaatatt
      121 gctgaaactt taagaccggt aattacttca ggaaaagatg tacctgattt gatttattta
      181 tcagtaggat ctgaaggtgg tttagtagat acaatgattt ctgataaagc aatcgctgaa
      241 atcagtgatg ttttagatat gaaagtttat ggtgaagatg taaaagttaa agataaaatt
      301 atccctggat ttactaattc atttgcaact aagccatatg gtgatggaaa attatattta
      361 gcaccattta cttatgatcc ttgcggctta ttctataatg ctggtttatt tgaacaaaaa
      421 ggttggacag ttccaacaac ttgggatgag atgtgggaat taggtgaaaa agcaaaggca
      481 gaaggaattg ctttattcac ttatccaaca actggatatt ttgatgcttt tttctcagcg
      541 ttattaaacg aaactgtagg tgctgacgct tatactaagt taatggaata tgatactaaa
      601 acatggcaat ctgctgaaac taaaaaagca tttgaaatag tcggaaaatt agcacaatat
      661 actgaagcta atacggttgc aaatgctaat aaagataatt tcacaaaaaa tcaacaatta
      721 attttagata ataaagcatt attctgtcca aatggtactt ggttgccaga tgaaatgtct
      781 gaagcaccac gtgctgaagg tttcaaatgg ggatttatgg cattaccaaa agtaagtgca
      841 gaaggagatt catattcttc tacattctct gaacaagtat atattccttc aaaagccaaa
      901 aatgctgatt tggctaaaaa atttattaca tttatgtaca gtgacgaagc tgcaaaatta
      961 tttgctgaag aatctggatg cgttttacca actacaactg cttcatcata tttaccagaa
     1021 aaagtaaaag ataaagatgg taatgaaatt gataatcaaa aaacactatt ctaccaaatt
     1081 tatgataatg gggctaagag ttgtacggtt ggatttaaat ctgttgatgc aattgaaggg
     1141 gttgatctaa ctagtgctga aggtattctt tatggaacag ttaattctgt tgtaacagga
     1201 gataaaacag ttgatgaatg gtataatgct gtgattgatg ctgttaaaaa atacgacaaa
     1261 taataattga taaaataata gcggtggtgt cattccaccg ctattttaga aagtaggtgc
     1321 agcaatgaat aagaaaaaag aaagacgcag attcattatt ttatgccttg ccccagcggt
     1381 aatcttattt ggagtgttca tgattttgcc gacccttaat gtcttttgga tgtcaacatt
     1441 aaaatggggc gggctatcag ccgataagac ttttgttgga tttaataatt ttgtgttatt
     1501 aatgcaagat atgaatttta ttcgtgcttt acaaaatacc attttgatta ttgcggttgt
     1561 aactgtaatt acgatggctg tagcaattct ttttgcttcg attttagtta gagaaaaaat
     1621 aaaaggacaa aatttctttc gggtaatttt ttatattcct aatatattat caatagtcgt
     1681 tatctcttcg attttttctg caatctatga tccctcagaa aatggtatga tcaatagtgt
     1741 tttaatgtta tttaaaccag aaagctggga aactgttaag tttcttggag atcaaagtat
     1801 tgttatttat gccatcatag gaacaatgat atggcaagct ataggttatt atatggttat
     1861 gtacatggct tcgatgagtt cgattcccga acacttgtat gaagcttcag cacttgatgg
     1921 agctgggaaa atcaaacaat ttttttcaat tacactacct ttgatttggg ataatatccg
     1981 aacaacttta actttcttta ttatttcgac gattaac
//
LOCUS       DAOMTA010000609         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2773_length_2016_cov_2.057624, whole genome shotgun sequence.
ACCESSION   DAOMTA010000609 DAOMTA010000000
VERSION     DAOMTA010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2773_length_2016_cov_2.057624"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLK68_13515"
     CDS             <1..897
                     /locus_tag="PLK68_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91464.1"
                     /translation="RYWITFNEINNVHTIPFAAGAIRHDSNLQDKFQAAHNMFVASSR
                     ANKLCHEIIPDAKIGCMLSLSGVYPATCKPEDVMGAYNLRRRSLFFSDVMIRGKYPSY
                     IKRIFEENNIQLDTRPEDFELIKDYPSQYLGFSYYRTTTYEDGMPILGNTGGVIGKAN
                     PYLKETPWGWQIDPLGLRYVCNELYDRYQVPLFIVENGMGNIDQIEVDGSINDDYRIA
                     YIRDHLEALKEAIKDGVDLIGYTYWGPIDIVSAGTGEMKKRYGFIYVDKDNQGHGTLK
                     RSKKKSFEWFKKVIASNGDDLS"
     gene            971..1633
                     /locus_tag="PLK68_13520"
     CDS             971..1633
                     /locus_tag="PLK68_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM91465.1"
                     /translation="MNLKLVIFDMDGLMYDTEQIGMDCLINAAQKFGYVIDQEFGLSS
                     IGMNANDYQKLVKEKFGADYPYDLISKKSRKTRMAYLRKNGMIIKPGLCELINYLQKK
                     EIKLALASSSSKETIDEYNHLAGFDNVFDYIIAGNMVEHSKPDPEIFLKVLEHFELKK
                     NEALILEDSRNGIMAAHNANIPVICVPDLVKHGQDITKLTYATLPSLNEVKLEIEKII
                     TK"
     gene            1665..>2016
                     /locus_tag="PLK68_13525"
     CDS             1665..>2016
                     /locus_tag="PLK68_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM91466.1"
                     /translation="MDGLMFDTEPLGAVCFARAAKQFGYIIEEEFRYKLIGINANDHY
                     ALMKSKFGQDCPAKEIHELSKKLRSDYLYKHGIVIKPGLFELITYLKNKGIKIAVASS
                     SAYSKINEYLALAGL"
BASE COUNT          717 a          236 c          387 g          676 t
ORIGIN      
        1 agatattgga ttacttttaa tgaaataaat aatgttcata cgattccttt tgctgctgga
       61 gcaatccgtc atgatagtaa tctacaagat aaatttcaag cggctcataa tatgtttgtt
      121 gcaagtagtc gagcaaataa gctgtgtcat gaaattattc cagatgcaaa gattggttgt
      181 atgttgtcat taagtggtgt ttatccagct acttgtaaac ctgaagatgt aatgggtgct
      241 tataatttac gtcgtcgttc attatttttc agtgatgtga tgattagagg taaatatcca
      301 agttatatta aacgaatttt tgaggaaaat aatattcagt tggacacacg tcctgaagat
      361 tttgaattaa ttaaagatta cccttcacaa tatttaggat ttagttatta tcgaacaaca
      421 acatatgaag atggaatgcc aattttaggt aatactggcg gtgtcattgg aaaagctaat
      481 ccatatttaa aagaaacacc atggggctgg caaattgatc ctttaggatt acgttatgta
      541 tgcaatgagc tttatgatcg ctatcaagta cctttgttta ttgtagaaaa tggaatggga
      601 aatattgatc agattgaagt ggatggctca atcaatgatg attatcgaat tgcctatatt
      661 cgagatcatc tcgaagcttt aaaggaagca attaaagatg gtgttgattt gattggatat
      721 acttactggg gtcctattga tattgtttca gcaggtactg gcgaaatgaa gaaacgttat
      781 ggttttatct atgttgataa agataatcaa ggtcatggaa ctttaaaacg ttctaaaaag
      841 aaatcatttg aatggttcaa aaaagttatt gcctctaatg gtgatgattt aagttaattg
      901 tattattaaa gaaaatatag taagataaag gagcgaaaaa acgctctttt attatattgg
      961 gggaagaatg atgaatttaa aattagtgat ttttgatatg gatggtttaa tgtatgatac
     1021 tgaacaaatt ggaatggatt gtttaatcaa tgcagctcaa aaatttggtt atgttattga
     1081 tcaagaattt ggattaagtt caattggcat gaatgctaat gattaccaaa aattagttaa
     1141 agaaaaattt ggtgctgatt atccttatga tctaattagt aaaaaatcac gtaaaacacg
     1201 aatggcttat ttaagaaaaa atggaatgat aatcaaacct ggtttatgtg aattaataaa
     1261 ttatttacaa aaaaaagaaa ttaagcttgc tttagcatca tcgtcaagta aagagacaat
     1321 cgatgagtat aatcatcttg ctggatttga taatgtcttt gattacatta ttgctggaaa
     1381 tatggttgaa catagtaagc cggatccgga gatcttttta aaagtgcttg aacactttga
     1441 gttgaaaaaa aatgaagctt tgattttaga agattctcgt aatggaatca tggccgctca
     1501 taatgcaaat atccccgtga tctgtgtacc tgatttagtt aaacatggtc aagatattac
     1561 taaattaaca tatgccacat taccttcatt gaatgaagtt aaattagaaa ttgaaaagat
     1621 aatcactaaa taaaacaatg atattggaat tggtgatttt tgatatggat ggtttaatgt
     1681 ttgatacaga accattggga gcagtctgtt ttgcaagagc ggctaaacaa tttggataca
     1741 taattgaaga agaatttcgc tataagctga ttggaatcaa tgccaatgat cattatgctt
     1801 tgatgaaatc aaaatttggt caagattgtc cggcaaaaga gattcatgaa ttatcaaaaa
     1861 aacttagaag cgattattta tataagcatg ggatcgtaat taaacctggc ttatttgaat
     1921 taatcacata tttaaagaat aaaggaataa aaatagcagt tgcatcttct tctgcctata
     1981 gtaagattaa tgaatattta gctttagctg gtttaa
//
LOCUS       DAOMTA010000610         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2776_length_2015_cov_1.506122, whole genome shotgun sequence.
ACCESSION   DAOMTA010000610 DAOMTA010000000
VERSION     DAOMTA010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2776_length_2015_cov_1.506122"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLK68_13530"
     CDS             <1..221
                     /locus_tag="PLK68_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91467.1"
                     /translation="SSLHKRYIPYYLTLLLAGKLEQGKLYYHQKLPYLTLKTRQQVNI
                     DGETYPSGTYNLKIVKNSLKIVLYRQKD"
     gene            408..>2015
                     /locus_tag="PLK68_13535"
     CDS             408..>2015
                     /locus_tag="PLK68_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM91468.1"
                     /translation="MKEYYNEAKTEVRKRVNGSLKPLTNEQVKANQEKYGLNELIETK
                     GKSIPVIFLEQFKDFLVIILIFAAIISGVLGDIESTLVILIVITINAILGTVQTVKAE
                     QSLNSLKELSSPSAKVLRDGKVIEIPSKEVTIGDEVYLEAGDFIPADGRILENASMKV
                     DESALTGESVAVEKSSDLITGEVALGDRTNMVYSGSFVTYGRGNFLVTGIGMETEVGK
                     IAQLLKSTSEKKTPLQVNLDNFGKKLSIIIMVFCALLFGINILQGGNVGDAFMFAVAL
                     AVAAIPEALSSIVTIVLSFGTQKMAREGAIIRKLQAVEGLGSVSIICSDKTGTLTQNK
                     MTVEDYYIEGKRIDASEIDPSIPLHKDLMRLSILCNDSSNVDGQEIGDPTETALINLS
                     AHLGVPASRVRAVYPRLSEIPFDSDRKMMSTLHLLKDGYTMITKGAVDVLIERIKYIR
                     KNNQIVPITAQDREDILAMNMEFSQNGLRVLAITYKKLTAEKSLDYDDENDLIFLGLI
                     SMMDPPRVESAPAVTECLQAGITPIMNT"
BASE COUNT          703 a          273 c          405 g          634 t
ORIGIN      
        1 tttcaagtct tcacaaacga tacatacctt attatttaac attactatta gcgggcaaac
       61 ttgaacaagg gaagctctat tatcatcaaa aattacctta tttaacatta aaaacaagac
      121 agcaggtcaa tattgatggt gaaacatatc cgtctggaac ttataatctt aaaattgtta
      181 agaattcgtt aaagattgtt ttatatcgac aaaaagattg actttcgaat gagtagagga
      241 taatattatg gttaataaaa gggagtagct aacacaatgt gtttacgatt tcgtcaatac
      301 gatggtttta aatcatccgg aacgtaatga aatagcgaga cttttattgc attttaagta
      361 tgcaataaag ggctcgcttt tattgtgttg agaaaaggag aaaatgaatg aaagaatact
      421 acaatgaggc caagacggag gtccgcaaaa gagtaaatgg ttcattaaag ccactaacta
      481 atgaacaagt taaagctaac caagaaaagt atggtttaaa tgagttaatt gaaactaaag
      541 gaaagagtat acctgtaatt ttcttagaac aatttaaaga ttttttagta attattctaa
      601 tttttgcagc tattatttct ggagtattag gtgatatcga aagtacgctg gttattttaa
      661 tcgttattac gattaatgct attttgggaa ctgttcaaac agtcaaagca gaacaatcat
      721 taaatagttt aaaagaatta tcttcaccaa gtgctaaagt cttacgagat ggaaaagtta
      781 ttgaaattcc cagtaaagaa gtaacgattg gtgatgaggt ttatttagaa gccggtgatt
      841 ttattcctgc agatggacgt attttagaaa atgctagtat gaaggtggat gaaagtgctt
      901 tgactggtga aagtgttgcc gttgaaaaaa gctctgatct aatcactgga gaagttgctt
      961 taggtgatcg cacaaatatg gtttattccg ggagttttgt tacctatgga cgtggtaatt
     1021 ttttagtaac aggaattgga atggaaactg aagtaggaaa aattgcacaa ttattgaaat
     1081 ctacttctga aaagaagaca ccacttcaag ttaatctgga taattttgga aaaaaactat
     1141 caattataat aatggttttc tgtgcattgt tatttggtat taatatcctt caaggtggta
     1201 atgttggaga tgcctttatg tttgctgtcg cactagctgt tgctgcaatt cctgaggcat
     1261 tgagttcaat tgtaacaatc gttttatctt ttggaactca aaagatggct cgagaaggag
     1321 caattattcg taaattacaa gcagttgagg gattgggcag tgtttctatt atctgctctg
     1381 ataaaactgg aacattgacc caaaacaaga tgactgttga agattactat attgaaggaa
     1441 aacgaattga tgctagtgag attgatccaa gtattccttt gcataaagat ttgatgcgtt
     1501 taagtatctt atgtaatgat tcatcaaatg ttgatggtca agaaatcgga gatccaacag
     1561 aaacggcatt aattaattta agcgctcatt taggagtacc agcaagtcgt gttcgggcag
     1621 tatatccacg tttaagtgaa attccttttg acagtgatcg aaaaatgatg tcaacccttc
     1681 atttattaaa agatgggtac acgatgatta caaaaggggc agttgatgtt ttgatcgaac
     1741 ggattaaata tattcgtaaa aataatcaaa tcgtaccaat aacggcacaa gatagagaag
     1801 atattttagc gatgaatatg gaattctcac aaaatggatt acgtgttcta gcgataactt
     1861 ataaaaaatt aactgctgaa aaaagtttag attatgatga tgaaaatgat ttgattttct
     1921 taggtctgat ttcgatgatg gatcccccac gagttgaaag tgcaccagca gtaacagaat
     1981 gtctccaagc gggaatcact ccaattatga atacc
//
LOCUS       DAOMTA010000611         2012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2780_length_2012_cov_3.756771, whole genome shotgun sequence.
ACCESSION   DAOMTA010000611 DAOMTA010000000
VERSION     DAOMTA010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2780_length_2012_cov_3.756771"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1308)
                     /locus_tag="PLK68_13540"
     CDS             complement(112..1308)
                     /locus_tag="PLK68_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRM91469.1"
                     /translation="MSKKDIVKYIIDEYGVTSPTDITNALKDLLGETLQDMLNSEFDE
                     HMGYDKYDQKTDKTNYRNGTSKKTVKTSQGNIDLDIPRDRNSSFDPAIIEKHNRDISD
                     VDNKIINLYARGMSTRDISDTVKDIYGVEVSAAMISKITDKIIPKALQWQNRPLDTVY
                     PIVFIDCVHFNVKTDNMVTKKAAYVVLGVNENGYKEIFGIWIGENETAKFWLSVLTDL
                     KNRGVKDILIICSDGLSGIKQAIESVFPNTVQQRCIVHLIRNSCKYLSYKDRKEFCKD
                     LRTVYTESTSDKALEALDKCKGKWGDKYPYAFKPWEENWNEVCSMFNYVPELRKIMYT
                     TNAIESLNSAFRKFTKIRTVFPTDESLFKSLYLAQDKITSKWNVPYGNWGIIYSSLQI
                     IFEGRV"
     gene            complement(1423..>2012)
                     /locus_tag="PLK68_13545"
     CDS             complement(1423..>2012)
                     /locus_tag="PLK68_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM91470.1"
                     /translation="INAIENLTATTLRNIIGDLELDETLTSRDIINSRMRSILDEATD
                     PWGIKVHRVEVKNIIPPRDIQEAMEKQMRAERERREAILQAEGKKTAAILNAEGDKES
                     MILRATADKEAKIAIAEGEAEALRLVYEAQAKGITYINQANPDSAYVTLQGFKALEEL
                     SKGEATKIIIPSEIQGIAGLASSLKELVTDKPKKD"
BASE COUNT          590 a          369 c          251 g          802 t
ORIGIN      
        1 aaaacataat ttatctaact tcttaaaaaa cttacacact tttattgaca aagcctcata
       61 cagttacttt tttatatcat atcataatat atttaattgt aaatgttctc gttaaaccct
      121 cccttcaaag atgatttgaa gactagaata tataattccc caattaccat atgggacatt
      181 ccatttactt gttattttat cttgagctaa gtacaaagat ttaaataaac tttcatctgt
      241 tgggaatact gttcttattt tagtaaactt tctaaatgca ctatttaaac tttcaattgc
      301 attcgttgtg tacatgattt ttcttagttc tggaacataa ttaaacatac tgcaaacttc
      361 attccagttt tcttcccatg gcttaaatgc atacggatat ttatctcccc atttaccttt
      421 acacttatcc agtgcttcta atgctttatc acttgtagat tctgtatata ctgttcttag
      481 atctttacag aattcttttc tgtctttata actcaagtat ttacatgaat ttctaattag
      541 atgaactatg catctttgtt gtactgtatt tgggaatact gattcaattg cttgtttaat
      601 acctgaaaga ccatcactgc aaataatcaa tatatcctta actcctctat tctttaaatc
      661 agttagtact gatagccaga atttagctgt ttcgttttcg ccgatccaaa taccaaatat
      721 ttccttatat ccattctcat taactcctaa aacaacatat gcagcttttt tggtaaccat
      781 attatctgtt ttaacattaa aatgaacaca gtcaataaag actattggat atacagtatc
      841 taatggcctg ttttgccatt gtaaagcttt agggataatt ttatctgtta ttttacttat
      901 catagctgca ctaacttcta caccgtaaat atctttaaca gtatcactga tatctcttgt
      961 agacatacca cgggcatata agttgataat tttgttatca acatcactaa tatcacgatt
     1021 gtgtttttct ataatagctg gatcaaatga tgagtttcta tctctaggaa tatctaaatc
     1081 tatatttcct tgagatgttt taactgtttt tttggaagtt ccattacgat aattagtttt
     1141 atctgttttt tgatcatact tgtcatatcc catatgttca tcaaattcag agttcaacat
     1201 atcttgtaat gtttcaccta ataagtcttt taaagcatta gttatatcag taggtgatgt
     1261 aacaccatac tcatcaatta tatatttaac tatatctttt ttactcataa taaaaatcct
     1321 cctttggtag ttaatttcat tctaccattg gaggatcagt ttacacaaaa ttatttacac
     1381 tctcttaaag gacgtcagta atatgacgtc cacaatgttt aattaatctt tttttggttt
     1441 atcagttact aattctttta aagaactagc taaaccggca ataccttgaa tctcactagg
     1501 aataataatt tttgtggctt cacctttaga taactcttct aaggctttaa acccttgtaa
     1561 tgttacatat gcactgtctg ggtttgcttg attaatatat gtgatacctt tggcttgggc
     1621 ttcataaact aatcttaatg cttctgcttc tccttcagca attgcaattt tagcttcttt
     1681 atctgctgtt gcacgtaaaa tcattgattc tttatcacct tcagcattta agatagcagc
     1741 agtctttttt ccttctgctt gtaagattgc ttcacgtctt tcgcgttcag ctcgcatttg
     1801 tttttccatt gcttcttgaa tatcccgagg tggaataatg tttttaactt caacacgatg
     1861 tactttgatt ccccatggat cagtagcctc atcaagaata ctacgcattc ttgagttaat
     1921 aatatctctt gatgtcaaag tttcatctaa ttctaaatcc ccaataatat ttcttaatgt
     1981 agttgctgtt aaattctcga ttgcgttgat tg
//
LOCUS       DAOMTA010000612         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2792_length_2007_cov_2.318648, whole genome shotgun sequence.
ACCESSION   DAOMTA010000612 DAOMTA010000000
VERSION     DAOMTA010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_2007_cov_2.318648"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1556)
                     /locus_tag="PLK68_13550"
     CDS             complement(147..1556)
                     /locus_tag="PLK68_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM91471.1"
                     /translation="MDYKEIADQIIITIGGQDNVKYVTHCVTRLRFVLKNKNLVNQDK
                     INEIDGVLGSTFGAGQFQIIMGKNLSATFTQVVKNYNFEVAEIIDEILDEDIKEKDDS
                     PIWKRGIKNIFNFLSACVTPMIPGLTVAGLIKVFLVLTSLAWPEIVDNQTYLVINGIV
                     NTSFYFMPVFVAYGAAIRLGSTPIYSMIVACLLIHPDIIGMLSAEEPLKILGFTAYSA
                     SYASSFLPAILSTVAVANIEKLLNKYLPGMFKGIFVGGLTLVLASLLTLTVLGPIGFF
                     AGEYFINFLVWMQSTIGPFALGALGGVLPFVIMSGMHTVFGPVMMQSIASLGYEGFLR
                     PTQFVHNVAEGGACFGVALKTKNPELRSQAISSGVGAILAGVSEPAIYGINLRLKKPM
                     YGVMAGGAVGGCIAGFFGVKAFAYGNPSILALPIYGETIIGIIIAIIAAFVVSSVVSY
                     FSGFEDVPVKNKNFQQNSH"
     gene            complement(1568..>2007)
                     /locus_tag="PLK68_13555"
     CDS             complement(1568..>2007)
                     /locus_tag="PLK68_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM91472.1"
                     /translation="EVGLKALDLINRYYQINLNKKEASAIAFHLINAEFNNNVSKTTS
                     ILKSIDDILNIIEANFGLELPEDSLYYSRLVIHLKFFMQRVIKGESDDENFEKLIISA
                     KSDINKKIGITLDSIERYLNEEFDYVLSEAERFYLLVHISRII"
BASE COUNT          692 a          396 c          244 g          675 t
ORIGIN      
        1 cattactttt tttgaaaata cattgtcatc aacatattca agattaaaac tgactccatt
       61 tacaactgca tatacattat ttgtattttt ttgattattt tttaatgatt taaatatctt
      121 tatcatagta ttaactccta aatttgctaa tgactatttt gttggaagtt tttatttttt
      181 actggaacat cttcaaatcc tgagaaataa ctcaccacac tagatacaac aaatgccgca
      241 ataattgcga taatgatgcc aataatcgtt tccccataaa taggaagtgc caaaatacta
      301 ggattaccat aagcaaatgc ctttactcca aaaaatccag caatacaacc gccaactgca
      361 ccaccagcca taacaccata cattggcttt tttagacgca gattgattcc gtagatagcc
      421 ggctctgata ccccggctag aatagccccg acaccactac taatcgcttg tgaacgtaat
      481 tctggattct tagtttttag tgctacccca aaacaagctc ctccctcagc aacattatga
      541 acaaattgtg ttggccttaa aaaaccttca taacctaatg atgcaatact ttgcatcatt
      601 acagggccaa aaacagtatg cattcctgac attattacaa atggcaatac accaccgaga
      661 gctccaagcg caaatggacc aatcgtgctt tgcatccaaa ctaagaaatt aataaaatat
      721 tcaccagcaa aaaaaccaat tgggcctaat acagtaagag ttaaaagact tgcaagaaca
      781 agcgttaatc caccaacaaa aattccttta aacattcccg gtaaatactt attcaaaagc
      841 ttttcaatat ttgcaactgc tacagttgat aatattgctg gtaaaaaaga tgaagcataa
      901 cttgctgaat aagctgtgaa gcctaatatt tttaagggct cttcagcact taacatccca
      961 ataatatctg gatggatcag tagacaagct actatcattg aataaatagg tgtacttcct
     1021 aatctgattg ctgcaccgta tgctacaaaa acaggcataa agtagaaaga agtattaaca
     1081 ataccattta taactagata tgtttgatta tcaacaattt ccggccacgc taatgaggtt
     1141 agtaccaaaa atacttttat taatccagct acagtaagcc caggaatcat tggtgtaaca
     1201 catgcagata agaaattgaa aatatttttt attcctcttt tccaaattgg cgaatcatct
     1261 ttttctttaa tatcttcatc caaaatttca tcaataattt ctgctacttc aaaattataa
     1321 tttttaacta cttgagtaaa tgtcgcagat aaatttttcc ccataattat ttgaaattga
     1381 ccggcaccaa aggttgagcc caacactcca tcaatttcat taatcttatc ttgattaact
     1441 agatttttat tttttaatac aaatcgtaat cttgtaacac agtgggttac atatttaaca
     1501 ttatcctgtc cgccaatcgt tataatgatt tgatcggcta tttctttata atccatatta
     1561 ttctcctcta tattattctt gaaatatgta ctaataaata aaatcgttcc gcctctgata
     1621 gtacataatc aaattcttca tttaaatatc tttcgattga atctaacgta atacctattt
     1681 ttttattaat atctgactta gcacttatta ttaatttttc aaaattctca tcatcacttt
     1741 caccctttat cacccgctgc ataaaaaatt taagatgaat cactagccta gaataataca
     1801 gtgaatcttc cggtagttca agaccaaaat tagcttcaat tatatttaaa atatcatcta
     1861 tgctctttaa aatactcgtt gttttgctta cattattatt aaactcagca ttaattaagt
     1921 gaaatgctat agcacttgcc tcttttttat ttaaatttat ttgataatat ctattaatca
     1981 aatctaatgc tttaaggccg acttcat
//
LOCUS       DAOMTA010000613         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2793_length_2007_cov_2.210553, whole genome shotgun sequence.
ACCESSION   DAOMTA010000613 DAOMTA010000000
VERSION     DAOMTA010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_2007_cov_2.210553"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLK68_13560"
     CDS             <1..1028
                     /locus_tag="PLK68_13560"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRM91473.1"
                     /translation="YKVAVVGATGAVGREMLRCIFQFKFPFESIKLLASARSAGKVVE
                     FEGHEFTVEELTENSFEGIDVAFWSAGGSISEKYMPFAVKAGCVNIDNTSHFRMDPEV
                     PLVVPEVNGEALRNHKGIIANPNCTTIQMVSALNRLNEYFDIERIIVSTYQAVSGAGV
                     AGMEELYRQSQEILDGKEPVAKTLPCAGDKKHFPIAFNCIPQVDKFDLDNHFSKEEMK
                     MVNETKKIFNKDIKVNATCVRVPVLRGHSESVYIETKKPIDIDKVFELLNNSTGVELY
                     DDIENQIYPMANLFVGDELVHVGRVRKDLDNPNGLSLWVVADQLMKGAAYNSVDIGLK
                     MIEMGLI"
     gene            1590..1910
                     /locus_tag="PLK68_13565"
     CDS             1590..1910
                     /locus_tag="PLK68_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRM91474.1"
                     /translation="MAKKLISIATLFVLMAVSVFSAFAVEDETKNALVYGDVNIDGDI
                     TVIDATDIQKYIVGLEELTADSKSVADVDSDGAISVMDATSIQKYIVGLNGCGKVGQQ
                     FVTE"
BASE COUNT          662 a          264 c          395 g          686 t
ORIGIN      
        1 aatataaagt tgcagtcgtg ggggctactg gagccgtcgg aagagaaatg ttaagatgta
       61 tcttccaatt taaatttcct tttgaaagta ttaaattatt agcatcagct cgtagtgcag
      121 gaaaagttgt tgaatttgaa ggtcatgaat ttactgttga agaattaaca gaaaatagct
      181 ttgaaggtat cgatgtagcg ttttggtctg ccgggggaag tatttctgaa aaatatatgc
      241 cattcgctgt taaagctggg tgtgtcaata ttgataatac tagtcatttc agaatggatc
      301 ctgaggtgcc attagtagta cctgaagtaa acggagaagc tttaagaaat cataaaggaa
      361 ttattgctaa tccaaactgc actactatcc aaatggttag tgctttaaat cgtttaaatg
      421 aatattttga tatcgaaaga atcatcgttt ctacttatca agcggtttca ggagcaggtg
      481 ttgcaggaat ggaagaatta taccgccagt ctcaagaaat cttagatggt aaagaacctg
      541 ttgccaaaac attaccttgc gctggggata aaaaacattt tccaattgct ttcaattgca
      601 ttccacaagt tgataaattt gatttagaca atcatttttc taaagaagaa atgaaaatgg
      661 ttaatgaaac taaaaaaatc ttcaataaag atatcaaggt caatgctact tgtgttcgtg
      721 ttcctgtact tcggggacat tctgaatcag tatatattga aactaaaaaa ccaatcgata
      781 ttgataaagt attcgagcta ctaaacaatt ctactggggt tgaattatat gatgatattg
      841 aaaatcaaat ctatccaatg gctaatttat ttgttggaga tgagttagta cacgttggac
      901 gtgttcgtaa agatttagat aaccctaacg gattaagttt atgggtagtt gccgatcaat
      961 taatgaaagg tgctgcttac aactctgttg atattggact taaaatgatt gaaatgggct
     1021 taatttaata aaaaaggaat tttattcgat ggtgatatgc cccttgttaa gtagataagg
     1081 taaataacca aaatctactt aaggaggagc gtaccatagc ttgaataaag ttccttttta
     1141 tttatttatt tatactgaca gaataacatt ctttgcaacg aaattttcgt atgataacta
     1201 aatggttata acagtggcaa aattcagtat tgctattgcg tttgtgatgc ataaattagt
     1261 gtgatgtgaa ccttattgta tgtatagaag atattaatgt aataaaaaca cattgttgtt
     1321 attcgttatt tgttaaattg ttatcttcat acaattctca tatttgttta taataaaaca
     1381 atttatcatc tttttgttta tttgctattg acagaacagt agtctttgtg ataaaatact
     1441 ttataagata accaaacggt tataaacgac aaagagaagt aaaagtatat tcttgttttc
     1501 tcgttcgatg tttatataat gttgattatc aaattcaatt tcggtatatt attggttgaa
     1561 aatgcactgt tctaaggaga gattttggta tggcaaaaaa acttattagt attgcgacat
     1621 tgtttgttct tatggcagta agcgtattca gcgcttttgc ggttgaggat gaaacaaaaa
     1681 atgctcttgt ttacggtgat gtgaacattg acggcgatat taccgtaatc gatgctaccg
     1741 atattcagaa gtacattgtc ggtcttgaag aacttacagc agacagtaag tcagttgcag
     1801 atgttgatag tgacggcgct atttctgtta tggatgcgac ttcaattcaa aagtatattg
     1861 taggtcttaa cggttgcgga aaagtcggtc agcaatttgt tactgaatga ttttaaatca
     1921 taatcacagt aataataata aagtcccgag gtattgtgtg tacttcggga ctttattatg
     1981 cattgaattt gtattattaa tagggct
//
LOCUS       DAOMTA010000614         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2808_length_2002_cov_2.312789, whole genome shotgun sequence.
ACCESSION   DAOMTA010000614 DAOMTA010000000
VERSION     DAOMTA010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2808_length_2002_cov_2.312789"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLK68_13570"
     CDS             complement(<1..819)
                     /locus_tag="PLK68_13570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRM91475.1"
                     /translation="MQKFEKANFNIANYDETDNYGKFVIEPLERGFGTTLGNSLRRVL
                     LSSLPGSAVYAIKVQGAIHEFSAVDGVVEDVTSIILNLKKLVFDVDSDESATMIIDVE
                     GPATVTGADIQCPSEVTMISNDMEIAHVAQGAHLYMELYAKKDRGYVSADQNKKEINT
                     IGIIPTDSIYSPVEKVSYAVEPTRVGESAKYDQLTLEIETNGALKPYEAISLAAKILV
                     EHLNMFVELTDMAVNMEVMSEAQSDTTNKVLDMTIEELDLSVRSYNCLKRAGIQT"
     gene            complement(869..1261)
                     /gene="rpsK"
                     /locus_tag="PLK68_13575"
     CDS             complement(869..1261)
                     /gene="rpsK"
                     /locus_tag="PLK68_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRM91476.1"
                     /translation="MAKVKQVRGKKRAKKNIAKGVAHVHSTFNNTIVTITDEHGNVLT
                     WSSAGALGFKGSRKSTPFAAQMASEAAAKASMDHGVKSVEVCVKGPGPGREAAVRALQ
                     AAGLEVTAINDVTPIPHNGCRPPKRPRG"
     gene            complement(1281..1646)
                     /gene="rpsM"
                     /locus_tag="PLK68_13580"
     CDS             complement(1281..1646)
                     /gene="rpsM"
                     /locus_tag="PLK68_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRM91477.1"
                     /translation="MARIAGVDIPRDKRVVISLTYIYGIGKPTAQEILKKVGINENTR
                     VKDLTEDEIGTIRKEIESIKVEGDLRREVALNIKRLMEIGSYRGIRHRKGLPVRGQKT
                     KTNARTRKGKAKPIAGKKK"
     gene            complement(1673..1786)
                     /gene="rpmJ"
                     /locus_tag="PLK68_13585"
     CDS             complement(1673..1786)
                     /gene="rpmJ"
                     /locus_tag="PLK68_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRM91478.1"
                     /translation="MKVRPSVKPMCDKCRVIKRKGRVMVICENPKHKQRQG"
     gene            complement(1818..>2002)
                     /gene="infA"
                     /locus_tag="PLK68_13590"
     CDS             complement(1818..>2002)
                     /gene="infA"
                     /locus_tag="PLK68_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRM91479.1"
                     /translation="VLETLPNAMFKVALENGVEILAHVSGKIRMHYIRILPGDRVTVE
                     ISPYDLTRGRITFRHK"
BASE COUNT          578 a          408 c          291 g          725 t
ORIGIN      
        1 tgtttgaatg cctgctcgtt ttaagcagtt atatgaacgt actgataaat ctaattcttc
       61 aatcgtcata tctaatactt tattagttgt atcactttga gcttcagaca taacttccat
      121 attcacagcc atatcagtta actctacaaa catatttaaa tgttctacta aaatcttagc
      181 agctaatgaa atagcttcat aaggttttaa agctccattt gtttcgattt ctaaagttaa
      241 ttgatcatat ttggcacttt ccccaacacg agttggttca actgcataag atactttttc
      301 aactggtgaa taaattgaat cagtaggaat aataccaata gtattaattt ccttcttatt
      361 ttgatcagca cttacataac cacgatcttt tttagcatat aattccatgt ataagtgagc
      421 accttgagca acatgagcaa tttccatatc attagaaatc attgttactt ctgaagggca
      481 ttgaatgtca gcacctgtta cagtagcagg tccttcaaca tcaataatca ttgtagccga
      541 ttcatcacta tccacatcaa agactaattt cttaagattt aagataattg atgtaacatc
      601 ttcgacaaca ccatctactg cagaaaattc atggatagca ccttgaactt taattgcata
      661 tacagcagat ccaggtaaag aagatagaag aactcgacga agtgaattcc ccaaagttgt
      721 accgaaacct ctttctaaag gttcaataac aaatttacca taattatctg tttcatcata
      781 attagctata ttaaaatttg ctttttcaaa cttttgcatc tatttccctc ctcactagga
      841 attattattt tttttgatca tacaataatt atccacgagg acgttttggt ggacggcacc
      901 cgttatgtgg aattggagta acatcattga ttgcagttac ttctaatcca gcagcttgta
      961 aagctctaac agctgcttca cgtcctggtc caggaccttt aacacatact tctacagatt
     1021 ttactccgtg atccattgat gcttttgcag cagcttcaga agccatttgc gcagcaaatg
     1081 gagtagattt tcttgaacct ttaaatccta atgcaccagc actagaccat gttaatacat
     1141 taccatgttc atcagtaatt gttacaatag tattattgaa agttgaatga acatgtgcta
     1201 caccttttgc gatattcttt tttgcacgtt ttttaccacg tacttgttta acttttgcca
     1261 tttctagttc aacctcctat ctattttttc tttcctgcga ttggttttgc cttaccctta
     1321 cgagtacgag catttgtttt agtcttttga ccacgaacag gaagtccttt acgatgacgg
     1381 attcctctgt agcttccaat ttccattaag cgtttaatgt ttaaagctac ttctcgacga
     1441 aggtcacctt caactttaat tgattctatt tcttttctaa ttgtaccgat ttcatcttca
     1501 gttaaatctt taactctagt gttctcgtta attccaactt ttttcaaaat ttcttgagct
     1561 gttggtttac cgataccata gatataagtt aatgagataa ccacacgttt gtcacgtggg
     1621 atatcaactc cagcaatacg agccatttta tttcctccta attaactact aattaacctt
     1681 gtctttgttt atgtttaggg ttttcgcaaa taaccattac gcgccctttt cttttaatta
     1741 ctctgcattt atcacacatt ggttttacag atggtcttac tttcatcttg tctacctcct
     1801 tttggaagat ttaagtttta tttatgtcta aatgtaattc gcccacgtgt taaatcgtat
     1861 ggagaaattt ctaccgtaac cctgtccccc ggtaaaatgc gaatgtaatg catacggatt
     1921 ttaccagaaa cgtgagcaag gatttctact ccattttcta acgctacttt aaacattgca
     1981 tttggtaatg tttcaagtac cg
//
LOCUS       DAOMTA010000615         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2817_length_1999_cov_1.987654, whole genome shotgun sequence.
ACCESSION   DAOMTA010000615 DAOMTA010000000
VERSION     DAOMTA010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2817_length_1999_cov_1.987654"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /gene="sbcD"
                     /locus_tag="PLK68_13595"
     CDS             complement(<1..406)
                     /gene="sbcD"
                     /locus_tag="PLK68_13595"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HRM91480.1"
                     /translation="MRFVHISDIHLGKLLYQQNLLEIQADLLNQVCDYLVDNEIDVLV
                     MAGDIYDRSVPSNEAIDVLNDFLTKVILKYHKKVLMIAGNHDSASRLSFVSGLLRQEG
                     LYIEAYPAKQMKPIEIEGVNFYLMPFFKPSYIR"
     gene            complement(418..822)
                     /locus_tag="PLK68_13600"
     CDS             complement(418..822)
                     /locus_tag="PLK68_13600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91481.1"
                     /translation="MQLEKQIYIDKDLPAGWKPYYIFLMKVNNEIVGRMTLREGSCEE
                     RYYDGHIGYTVEPEFRGHYYAYQGVQLIKPIALKLGFKELIITCSPNNLASKKTILKL
                     QAQYLETVEIPKKYRKDFEAGETIKEVYLIKL"
     gene            972..1274
                     /locus_tag="PLK68_13605"
     CDS             972..1274
                     /locus_tag="PLK68_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91482.1"
                     /translation="MKFKRLDRVDYQVSIITIIIVCASFFCVYIFNYKITHDDMIYSL
                     KERSNTIYHYVENYLDKDTFNHNFSLDMKNDTYKQIKQKCKKCNKCFISLYCKNDR"
     gene            1264..>1999
                     /locus_tag="PLK68_13610"
     CDS             1264..>1999
                     /locus_tag="PLK68_13610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM91483.1"
                     /translation="MTDEGEYIYLVDGLPTASPDFRYPGDKIEKEIIPELKAALKNKI
                     ILPDQIKETTWGPIFVSYYPIHDEGKVVGVLGIEFDASHQFEAFKQIRIITPLIAIMA
                     SLIATIIAVLSFRRISNPRFKDMANTDYLTNLHNRNAFEIDFENLSTHKHNTGIIITD
                     LNDLKKINDTYGHSTGDEYIKKVAKILEKHVQKNTVYRFGGDEFIILIHHDAKQQIKA
                     VVTAIQTELSKQTDEKLSISIGFAVYD"
BASE COUNT          712 a          334 c          259 g          694 t
ORIGIN      
        1 agcgaatata agatggttta aaaaaaggca ttaaataaaa attaacccct tcaatttcta
       61 tcggcttcat ttgtttagcc ggatatgctt caatatacaa tccttcttgt cttagcaatc
      121 cactaacaaa acttaatcga ctcgcactat catggtttcc cgctatcatc aaaacttttt
      181 tgtgatactt taaaataact tttgttaaaa aatcatttaa aacatcaata gcttcattac
      241 taggaacact ccgatcataa atatctccag ccatgactaa aacgtctatt tcgttatcga
      301 ctaaataatc acaaacttga ttcagtaaat cagcttgaat ttctaataaa ttttgttgat
      361 ataacaactt tcctaaatga atatcactaa tatgaacaaa tctcattatt ttcctcctta
      421 taatttaatt aaataaacct ctttaattgt ttcaccggct tcaaaatctt tccgatattt
      481 tttaggtatt tctactgttt ctaaatattg tgcttgtaat tttaaaatcg tttttttaga
      541 agctaaatta tttggtgaac aagtaattat cagttcctta aaccctagtt ttaaggctat
      601 tggttttatt aactggactc cttgatacgc ataataatgc ccacgaaatt cgggctcaac
      661 agtataacca atatgtccat catagtatcg ttcttcacaa ctaccttctc gtaaagtcat
      721 tcgccccaca atttcattgt ttactttcat taaaaaaata taatatggtt tccagccagc
      781 tggtaaatct ttatcaatat atatttgttt ttctaactgc atctctatca ccttaacaca
      841 ttatatcata aataccattt tgatgaggat gaatattatt actttgactg acaatatttg
      901 gttttataat cagcatatct aaacacaatt tagtcaattt atggtagaat caaagaaagg
      961 ggttgaaacc aatgaaattt aaacggctcg atcgggtaga ttatcaagta tcgatcatta
     1021 caatcataat tgtttgtgca tccttttttt gtgtctatat ttttaattat aaaatcactc
     1081 acgatgatat gatctatagt ctaaaagaac gttcaaacac gatatatcat tatgtagaaa
     1141 attatttaga caaagatact tttaatcata atttttctct tgatatgaaa aatgatactt
     1201 ataaacaaat aaaacaaaaa tgtaaaaaat gcaacaaatg ttttatatct ctatactgca
     1261 aaaatgacag atgaaggcga atatatctat cttgtcgatg gactgccaac tgcaagtcca
     1321 gattttcgtt atcctggaga taaaattgaa aaagaaataa ttcctgagct taaagcagca
     1381 ttaaaaaata aaattatttt gccagatcag attaaggaaa ctacctgggg tcccattttt
     1441 gtttcatatt accctataca cgacgaagga aaagtagttg gcgtgctagg aattgaattt
     1501 gatgctagtc atcaatttga agcctttaaa caaattcgaa ttattacacc gttgattgcc
     1561 attatggcta gtcttatcgc tactatcatt gccgttctat catttagaag aatttcaaat
     1621 cctcgtttta aagatatggc taatactgat tacttaacaa atttacataa tcggaatgct
     1681 tttgaaatag attttgaaaa cttatcaaca cataagcaca ataccggaat aattattacc
     1741 gatcttaatg atctaaaaaa aatcaatgat acatatggac atagtactgg tgatgaatat
     1801 attaaaaaag ttgctaaaat tcttgaaaaa catgtccaaa aaaatacagt ttatcgtttt
     1861 ggcggggatg aatttatcat tcttattcat catgatgcca aacagcaaat caaagctgtt
     1921 gtaacagcaa ttcaaacaga gctttcaaaa caaactgatg aaaaattatc aatctctatt
     1981 ggttttgctg tttatgatc
//
LOCUS       DAOMTA010000616         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2826_length_1993_cov_2.411249, whole genome shotgun sequence.
ACCESSION   DAOMTA010000616 DAOMTA010000000
VERSION     DAOMTA010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2826_length_1993_cov_2.411249"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(962..>1993)
                     /locus_tag="PLK68_13615"
     CDS             complement(962..>1993)
                     /locus_tag="PLK68_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRM91484.1"
                     /translation="KFYLNIAQLFAILKNGHLYIEIPINERLKYTNEGGKIMPVRLYL
                     DYGNLIVNFPYRDCGIARGDTITSINDVCSNEIVEKLYSLQGSEINNDLKDKGLEQYI
                     TPLLWYIYGWGDEYKFGIKNHGYIKTIYASGVPNQEAIKMINTHKAINRDFRVDYLDK
                     DSAIMTIPNFYQTKELSVFCDSVFKQLRDKKIKLLYINVRDNLGGKSSNVELLLSYLR
                     HPAYDSFRKVIIRVSKETIIYYKRMDQDIYNEIKDLPNGKFFELKSDKTKSNKHNSNI
                     FKGKVIVFANYRTYSAASYFVHMIAKHHIGNVIGTTGCPSVCSGNPIQYSLPNSHLNC
                     YIPTGLFCE"
BASE COUNT          656 a          294 c          290 g          753 t
ORIGIN      
        1 catctatgga taagttaaca gcttctcgaa gtaatttttc atctatatga tcttcgaatt
       61 gaataaagaa cttaatgcgt ggttgtccct cagacttata gaaatattgc atgaatgcaa
      121 aaggttgaac tttaaagtac ttgtttatat tacgactcat cgtttatatt attagttcac
      181 aaactttaga acctacaaag ttattatttt tatgattaaa atgaacttga tatgtctttt
      241 tatttctttt atattgtgat attggtgcat aatataatga ttattgaatc tagttttgcc
      301 ttcagcgtgg atttaaagca ttgattacca atgtgtttgg ctgaaggcaa tttttatggt
      361 ctgtaattat caataaatta aatccttttc tgtcgtagtc taattatatg atatatgagt
      421 acataatttg ttgtaattgt gtattattgt atataaaacg gtgtatgttt tgaaaaatat
      481 ttataatgaa ttactaaaaa tagcatagct aatttataat gaaagcatgt aaattaaatc
      541 gtatatgcta tccttttata actttcgatt aatgtgaaac aaactttcga cctaggtcgc
      601 acgagcgttt gacctaggtc gaaagtttgt agaggatagt tgttagagca taataccatt
      661 ccttttgagt attgaaaata acccttttat tagttactta ttattatgtt gttgttgtcg
      721 gtatttatta caatatacat aaagacggtt cagaaaaata aaattttatt ttactgatta
      781 gtcttaagtt ttgccttcag cagaatgcgc tgataatcaa tgctgtaagt cttgtttgaa
      841 ggcaaaacga aaataacttt taaactttct accatgcaca acaatgcatc gtaaattaat
      901 taatatataa ttttgtcaaa acgaaagagc aatattgcaa agcggttatg ggataatatt
      961 cttattcaca aaataaacct gtaggaatat aacaatttaa atgagaatta ggcaatgaat
     1021 attgtatagg atttccagaa catacactag ggcaccctgt tgttccgatt acatttccta
     1081 tatgatgttt agctatcata tgtacaaaat aagaagccgc agaataagtc ctgtaatttg
     1141 caaatactat aactttaccc ttaaatatat tcgagttatg cttgttagat ttggtcttat
     1201 cagacttaag ctcaaagaat ttaccattag gcaaatcttt aatctcgttg tatatatctt
     1261 gatccatacg tttataataa ataatagtct ctttactcac tctaataatt acttttctga
     1321 atgaatcata agcagggtgt cgtaaatagg aaagtaataa ttctacatta ctactttttc
     1381 cacccaaatt gtctcttaca tttatataaa gaagttttat ctttttgtca cgtaattgct
     1441 taaaaactga atcacagaag acagacaatt cttttgtttg gtagaaattt ggtattgtca
     1501 ttattgctga gtctttatca agataatcca ctctaaaatc ccggtttata gctttatgtg
     1561 tgttgatcat ttttatagct tcttgatttg gaacgcctga agcataaatc gtttttatat
     1621 aaccatggtt ctttattccg aatttatatt cgtctcccca accatatata taccatagta
     1681 gtggcgttat atattgttcc aatcctttat ctttaagatc attattgatt tcagaacctt
     1741 gcagagaata aagcttttcc acaatttcat tagagcatac gtcatttata ctagttatcg
     1801 tatctccccg tgcaatgcca caatctcggt atggaaaatt tacaataaga tttccataat
     1861 ctaagtataa tcttacaggc attatcttac caccctcatt agtatattta agtctctcat
     1921 taataggtat ttcgatgtac aaatgtccat tctttaatat agcaaatagt tgagctatat
     1981 tgagataaaa ttt
//
LOCUS       DAOMTA010000617         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2835_length_1989_cov_2.012927, whole genome shotgun sequence.
ACCESSION   DAOMTA010000617 DAOMTA010000000
VERSION     DAOMTA010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_1989_cov_2.012927"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLK68_13620"
     CDS             <1..345
                     /locus_tag="PLK68_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRM91485.1"
                     /translation="DTNIRTMSIDFNYWLIRFEETIAKSDYSFLHDCFDDDYLIFEED
                     LAKLETDYTKIDLYTKVHFVGCGLENDEIYRNFLEKFAIVSKNEHDSYYVKAKNQKQD
                     LNLLHDLVVRSD"
     gene            338..1165
                     /locus_tag="PLK68_13625"
     CDS             338..1165
                     /locus_tag="PLK68_13625"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRM91486.1"
                     /translation="MIKIDIFTNLITPFDIDDMIDYQALDNHIEKLVNQGNNKFIIGS
                     RTGEAASLTFLEQKHLLRYVCYHYPGLEIYMQLSESCTKKVIKQIGDLKDISEFAGYM
                     IELPEIFSLTQNGLIRHLDLIATATNKSIVIQQHKQNMIAVNHLLELKEKHENITGLI
                     TEVNSTGYQIIRNNGLGLYGIEEMLVQNEINNYDGLISNLTNLSYKKYRELITQHNDL
                     YFDFFKLIVKYFYQDNIVSTLKYSLSQSSNILYKLRLPLVKISDESKEQLNKIMEKY"
     assembly_gap    1287..1296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1341..1535
                     /locus_tag="PLK68_13630"
     CDS             1341..1535
                     /locus_tag="PLK68_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91487.1"
                     /translation="MKFAFIIMGDFNLKYDHAAIHQQTAQIIGVTNLAEASIVVKNYL
                     LRELTASNYAVLLEKQELKL"
BASE COUNT          769 a          205 c          334 g          671 t
ORIGIN      
        1 gatacaaata tccgaacaat gtcaattgat tttaattatt ggctaattcg ttttgaagaa
       61 acaatagcta aaagcgatta tagtttcctt catgattgct ttgacgatga ttatttaatt
      121 tttgaagaag atttagctaa actagaaact gattatacta aaatagatct ttatacaaaa
      181 gtacattttg ttggttgtgg attagaaaat gatgagattt atcggaattt tcttgaaaaa
      241 tttgcaattg tttctaaaaa tgaacatgat agttattatg tgaaagcaaa aaatcaaaaa
      301 caagatttga atcttttaca tgatttggtt gtaaggagtg attaaaatag atatttttac
      361 taatttaatt actccctttg atattgatga tatgattgat tatcaagcgc tagataatca
      421 tattgaaaaa ttagtaaatc aaggaaataa taaatttatt attggtagca ggacagggga
      481 agctgcttca ttgacttttt tagaacaaaa acacttatta cgatatgttt gctatcacta
      541 tcctggtctt gaaatatata tgcagcttag tgaatcatgt acaaaaaaag taattaaaca
      601 gataggagat cttaaagaca ttagcgaatt tgctggctat atgatcgagt taccagaaat
      661 ttttagttta acccaaaacg gattgattag gcatcttgat ctaattgcta ctgcaactaa
      721 taaatcgatt gttattcaac aacataagca aaatatgatt gctgttaatc atttattgga
      781 attgaaagaa aaacatgaaa atataactgg cttgattact gaagtaaata gcacaggtta
      841 tcagattatt agaaataacg ggttaggtct atacggtata gaagagatgc tagtgcaaaa
      901 tgaaattaat aattatgatg gacttattag taacttaact aatctaagtt ataaaaagta
      961 tagagaatta attactcagc ataatgattt atattttgat ttttttaaat taatagttaa
     1021 atatttttat caagataata ttgtaagtac attaaaatat tcgttgagcc aaagtagcaa
     1081 tattctttat aagttacgtt tgccattagt taaaatcagt gatgagtcaa aagaacaatt
     1141 gaataaaata atggagaaat attaatttat ttctccatta tttttgaatc acgaaataaa
     1201 aaaagatact ttaaatttat ttatttaatg aggcggatgt tatatactat taaaaacggc
     1261 tagataacta tgaaaaagta gatactnnnn nnnnnnagtc taattaaaga ttagagtttg
     1321 aattttaagg agacaatata atgaaatttg catttataat catgggagat tttaatttga
     1381 aatatgatca tgccgcaatc catcaacaga cagcacaaat aattggcgtt actaatttag
     1441 cagaagcaag tattgtggta aaaaattatt tattgaggga gttgactgca tcgaattatg
     1501 cggtgctttt ggaaaagcag gagctaaaac tataattgaa gtgacggaaa ataaaatacc
     1561 aattgggtat gttacgcatt taccagaaca agatgaaatt tacgatctca cttttgctaa
     1621 ataatttctt aaatgaacac ggctaaaaaa ggttattgtg ttaataatat tacatgttaa
     1681 agatgttagt aaaaattaaa tcatttctct tttcttgaga attttgatac aatgaacgta
     1741 gaaagtatta gcaaacgata ctataatatg ttaatatggt aaagattgtt tttcagcgat
     1801 gattaagaaa taatatagat tataataatg gaggaaaaaa tgaagaaaga tgttgttaaa
     1861 attttaccac ttggcggaca ggctgagatg ggtaaaagta tgtactgcat cgaaataaaa
     1921 gaacagattt ttattttaga tgccgggttt agattcccag aagtaagtaa attaggagtg
     1981 gatatcatt
//
LOCUS       DAOMTA010000618         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2838_length_1988_cov_1.894982, whole genome shotgun sequence.
ACCESSION   DAOMTA010000618 DAOMTA010000000
VERSION     DAOMTA010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_1988_cov_1.894982"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="PLK68_13635"
     CDS             <1..1650
                     /locus_tag="PLK68_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HRM91488.1"
                     /translation="VMIVKDGVDYQSLDSQPARLFFMIAAPVDGGNIHLETLARLSGM
                     LMDNDFKENLINASDAVEFLRLIDDKENEIVKVKEETDGTNYEVLAVTACPTGIAHTF
                     MAAESLEAKAKEMNILFKVETNGATGPKNVLTAEEISKARCIIVAVDKQVEMSRFDGR
                     PVIITKVADGINKAEELLSQAINGNVEIYHHQGDKVVSSSANEGIWRKLYTQLTDALS
                     KILPIIIGSGALISIASLAASYNLFGIKQIYDSIWLSNSYILGITVNGMIVALFAGFI
                     GQAIASQQGFAVALAGGAAMLLNNMYLQNMPSPGILGAIIAGFIGGYVVILLQKICRK
                     LPECLDGIKPTVIYPIFGVMITGVLSYLISPYVGSLNQTISVFIGSMDIVYKLILGVI
                     VGGMMSVDMGGPVNKIAYLFGIAQIVEGNFDVMAAVMAGGMVPPLAIAISTTFFRNKF
                     TLTERKLGCQNYLKGLMFISEGTIPFVQKDPRLVTLACIVASAVAGGFSMLYNCGIRI
                     PHGGIFVLPLIAHPFRYIVALLAGSLCGAIIYGFFKEIGEE"
     gene            1619..>1722
                     /locus_tag="PLK68_13640"
     CDS             1619..>1722
                     /locus_tag="PLK68_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRM91489.1"
                     /translation="MDSLKKLEKNDMKYIFFDIDGTLTDNTTGKIVPSA"
     assembly_gap    1723..1745
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1746..>1988
                     /locus_tag="PLK68_13645"
     CDS             <1746..>1988
                     /locus_tag="PLK68_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM91490.1"
                     /translation="KLQENGNFVAIATGRAYYKAKNFLKEVGLNNMVCNGGNGLVINH
                     QLVKNAPLDRQKALAVIDEAENLGYGILIAPFDSIDV"
BASE COUNT          637 a          236 c          425 g          667 t
ORIGIN      
        1 gtaatgatag ttaaagatgg tgtggattat caatcattag atagtcaacc agcacggttg
       61 ttttttatga ttgcagcacc agttgatggt ggtaacattc atttggaaac attagcaaga
      121 ttatccggga tgttaatgga taatgatttt aaagaaaact taattaatgc tagtgatgca
      181 gtggaatttt taagattaat tgatgataaa gaaaatgaaa tagtgaaagt aaaagaagaa
      241 actgacggta ctaattatga agttttagca gttaccgcat gtcctactgg aattgctcat
      301 acttttatgg cagcagaaag tttagaagca aaagctaaag aaatgaacat attatttaaa
      361 gtagagacaa atggggcaac agggcctaaa aatgttctta cggctgaaga aatcagtaag
      421 gcacgatgta ttattgtggc agtagataaa caagtggaga tgtcacgttt tgatggtcgg
      481 cctgtaatta taactaaagt agctgatgga attaataaag cagaggagtt attgtcgcaa
      541 gcaataaatg gtaatgtaga aatttatcat catcaaggag ataaagtggt tagtagttct
      601 gccaatgagg gaatttggcg aaaattatat acccaattga cagatgcttt aagtaaaata
      661 ttgccaatca ttattggatc gggagcattg atttcaattg cttcattagc agcaagttat
      721 aatttatttg gaatcaaaca aatttatgat agtatttggc taagtaacag ttatattctg
      781 gggataaccg ttaatgggat gatcgttgct ttatttgctg gctttattgg tcaggcaatt
      841 gcaagtcagc agggatttgc agtagctctt gctggcgggg ctgcaatgtt attaaataat
      901 atgtatctac aaaatatgcc tagtcctggt attttaggtg caatcatagc gggctttatt
      961 ggtggatatg ttgtaatttt attacaaaaa atttgtcgta aattgccaga atgtttagat
     1021 ggtataaagc caacagtaat atatccgatt tttggggtca tgattacggg tgttttatca
     1081 tatttgatta gtccttatgt tggaagtcta aatcagacta ttagtgtatt tattggtagt
     1141 atggatattg tttataaact aattttagga gttattgttg ggggaatgat gtctgttgac
     1201 atgggtggcc cagttaataa aattgcttat ctttttggaa tagctcaaat tgttgaaggt
     1261 aactttgatg ttatggctgc tgtaatggcc ggcggaatgg taccgccatt agcaattgca
     1321 attagtacaa ctttctttag aaataaattt acattaactg aacgtaaact aggttgtcaa
     1381 aattacttaa aaggcttaat gtttatttct gaagggacaa ttccttttgt tcaaaaagac
     1441 ccccgcttag taacattagc ttgcattgtt gcttcagcag tagctggtgg tttttcaatg
     1501 ctttataatt gtggaattag aattcctcat ggtggaatct ttgtattacc attaatagcg
     1561 catcctttta gatatattgt tgctttatta gcaggaagtc tttgtggagc tattatttat
     1621 ggattcttta aagaaattgg agaagaatga tatgaaatat atattttttg atatcgatgg
     1681 aactttgacg gataatacaa ctggaaaaat tgttccaagt gcnnnnnnnn nnnnnnnnnn
     1741 nnnnnaaatt acaagaaaat ggtaattttg tagctattgc aacgggacgt gcttattata
     1801 aagctaaaaa ctttcttaaa gaagttggtt taaataatat ggtttgtaat ggtggtaatg
     1861 gtcttgtcat aaatcatcag ttagtaaaaa atgcaccatt ggatcggcaa aaagcattgg
     1921 ctgttattga tgaagcagaa aatttaggct atggaatcct aatagctcca tttgatagta
     1981 ttgatgta
//
LOCUS       DAOMTA010000619         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2839_length_1987_cov_3.459110, whole genome shotgun sequence.
ACCESSION   DAOMTA010000619 DAOMTA010000000
VERSION     DAOMTA010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2839_length_1987_cov_3.459110"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1134)
                     /locus_tag="PLK68_13650"
     CDS             complement(241..1134)
                     /locus_tag="PLK68_13650"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRM91491.1"
                     /translation="MHRLAEYEAIQKLSSTKHYPIDRLCKYLNVTRSAYYRWGKYPKS
                     NNELRNERLSTEIQRIHHQHPDMGYRRIRDELDGHKGIHVNDKRVLRICRKYDIKSNI
                     KWKPKSCTRGDRNPDHIAKNYLHREFHAEKPNEKWLTDVSEFKYYNGIEVHKVYLSAI
                     LDLYDRRIVSFKISDHNDNPLVMDTFDEAVRQEPDAHPLVHSDRGFQYTSAQFYTRLK
                     KHHMKQSMSRVAHCIDNGPMEGFWGILKREMYYKQRFNDRSSLITAIANYIDYYNNQR
                     LQRKLHVMTPMKYHEQYTKAA"
     gene            complement(1134..1820)
                     /locus_tag="PLK68_13655"
     CDS             complement(1134..1820)
                     /locus_tag="PLK68_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91492.1"
                     /translation="MFRKSKIEPVEKVKIVERYLAGEIGIQQAGKELGVDHHSIRNWI
                     SIYQYDGPTGLLNQPKNKSYSKDLKISAINDYLNGEGSLQDICTKYGIRSHRQLSDWI
                     KVYNSGGILKTSTGGAYMKKAKNTTLDERLKIVTDCLANDKNYGAMALKYDCSYQQVR
                     NWVIRYEKMGQAGLEDRRGRRIASLPSRTPEEELRDKIAELERRNLDLQMENDLLKKV
                     RELERRGRYL"
BASE COUNT          543 a          392 c          333 g          719 t
ORIGIN      
        1 gtacgtgcga gacgtatcat tgacggaacg gatgccggac gccatatggt attgaatacc
       61 cttgaaagat tgaactatct ttaaaatatg tccctaaaag ggaacccctt tccaatataa
      121 atataaatac aaaaaaagcc tcggagaatt atttacttat gattggtatg ctccccgtca
      181 agaggacaat aaaaaataat aaatttttgt atcgtcagcc atgctatggc tggcgatatt
      241 ttatgctgct tttgtatatt gctcatgata tttcattggt gtcattacat gcagtttcct
      301 ttgaagtcgt tggttgttgt agtaatctat gtaattagct atcgcagtaa tcaaagagct
      361 tctgtcatta aaacgctgtt tataatacat ttctcgtttt agaattcccc aaaacccttc
      421 cattggtcca ttgtcaatac agtgggcaac tctagacata ctctgcttca tatgatgttt
      481 ttttaatcta gtataaaact gtgcgcttgt atattgaaag cctcgatcag aatgaactaa
      541 tgggtgtgca tctggctcct gacgaacagc ctcatcaaac gtgtccataa ctaatggatt
      601 atcattatga tcgctgattt taaaggaaac aatccttcta tcatataaat ctaaaatagc
      661 actcagataa actttatgaa cttctattcc gttgtagtat ttaaattcag aaacatctgt
      721 aagccacttt tcattcggtt tttcggcgtg aaattcacgg tgtaaatagt tttttgctat
      781 gtgatcagga tttctgtctc ctctggtaca actcttaggt ttccatttaa tatttgattt
      841 tatatcgtat ttcctacaaa tacgaagtac tcgtttatca ttcacatgaa ttcctttatg
      901 tccatctaat tcatcacgaa tccttcggta tcccatatct ggatgctggt gatgaatcct
      961 ttggatttca gtggatagtc tttcgtttcg aagttcatta ttacttttcg gatattttcc
     1021 ccagcggtaa tacgcagaac gtgtaacatt aagatattta caaagtctat ctatgggata
     1081 atgcttagta gacgatagtt tttggatggc ttcatattcg gctaaccgat ggattaaaga
     1141 tagcgacccc tcctttccag ttctctgact ttttttaaca gatcattctc catttgtaag
     1201 tctagatttc ttctttccag ttcagctatc ttgtcacgta gctcttcttc aggtgttctg
     1261 cttggaagag aagctatacg acgtccacgt ctgtcttcca gacccgcttg acccatcttt
     1321 tcgtatcgta ttacccaatt gcgaacttgt tgataggaac aatcatattt gagtgccatt
     1381 gcaccataat tcttatcatt tgcaagacaa tctgttacta tttttaatct ctcatcaagt
     1441 gtagtgttct ttgccttttt catgtaagca cctccagtgg atgtttttaa tattccacca
     1501 gaattataca ccttaatcca atcggaaagc tgtctgtgtg aacgaattcc gtattttgtg
     1561 caaatatcct gaagagatcc ttctccgtta agataatcat ttatagcaga tatttttaaa
     1621 tcttttgaat aagatttgtt tttcggttga ttgagtaatc cagttggtcc atcatactga
     1681 taaatagaaa tccaatttcg aatactatga tgatcaactc ccaattcttt tcctgcttgc
     1741 tgtatgccaa tttctcctgc aaggtagcgt tcgactattt ttactttttc tactggttct
     1801 atcttactct ttctgaacat gaaaataccc ccatatagga cttttatatt tcattgtcct
     1861 atatgagggt agcatatcat ttcctggggg atttttttgt ttaatacatg aagcggctga
     1921 cgcccttaca acttcttcat cgtagaatca tagtccgaaa caggatacca agtttatctt
     1981 gtggacc
//
LOCUS       DAOMTA010000620         1985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2847_length_1985_cov_1.681347, whole genome shotgun sequence.
ACCESSION   DAOMTA010000620 DAOMTA010000000
VERSION     DAOMTA010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_1985_cov_1.681347"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLK68_13660"
     CDS             <1..1609
                     /locus_tag="PLK68_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM91493.1"
                     /translation="TLDTIKYLISKDIDASKIAVLLRKNGNIVKTANYLSEHLEGVKI
                     VSDEAFRLDASISVLILVESLQLLAHPEDRLAKSFLVKAFQKQILCNELSDNEIFVSD
                     KAMDELLPESFILNFEKLKTISLYDLVEQLYKIFSLDKLPGQSAYICTFFDQISSFVS
                     ESSTDIDDFLEEWNNSICSKTIQSDELDGIRLITIHKSKGLEYDNVIVPFCDWNLDSR
                     DTIWCEPNIEPFSQLPIVPIDCSAKNMVGTIYEKDYINESLQNTVDNLNLLYVAFTRA
                     KNNLFVIGKKDTYANNRSQLIQATLESLINSMPGCTIKNNYNGDNLLFEYGSICIKEM
                     IVGNTSKNVFLQETKPESIEIKSFDNPVGFRQSNKSIEFIENEEDDEEFSQNNYIQIG
                     NILHHLFSTIRNINDIDPVLHQFQSEGLLYDESITKDKLSKMIGLRLKDKRVEDWFSP
                     HWTVFNECSIISVDKETNEIIEKRPDRVITDGKQIIVIDFKFGKQNYKYQYQVKEYMN
                     LLVGMGYKSIKGYIWYVYSNQINEIEL"
     gene            1606..>1985
                     /locus_tag="PLK68_13665"
     CDS             1606..>1985
                     /locus_tag="PLK68_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91494.1"
                     /translation="MKKTFLQYTAESLLEKFNCDLSGVTVVFPNKRASLFFNEYLARA
                     AKRPIWSPSYITISELFRQHSKLTVGDPIKLICDLHKSFVRQTGIDETLDRFYGWGQL
                     LLADFDDIDKNMADADKVFQNLSD"
BASE COUNT          730 a          281 c          334 g          640 t
ORIGIN      
        1 gacacttgat acaatcaaat atttaataag caaagatatt gatgcttcaa agatagctgt
       61 tcttttaagg aaaaatggga atattgtaaa gactgcaaat tacttatctg aacatttaga
      121 aggagttaag attgtttctg atgaagcttt tcgcctagat gcttctattt ctgtattgat
      181 tcttgttgaa tcattgcagt tacttgcaca tccagaagat cgcttagcaa agtcattttt
      241 agttaaagct ttccaaaaac agatattgtg taatgagcta tctgacaatg agatatttgt
      301 atctgataaa gcgatggatg aattattgcc ggagtctttt atacttaatt ttgaaaaact
      361 caaaaccatt tcgttatacg accttgtaga gcaattatat aaaatattct ctttagacaa
      421 attgcctgga cagagcgcat atatctgtac ctttttcgac caaataagtt cttttgtatc
      481 tgaaagtagt acagatattg atgattttct agaagaatgg aataattcca tctgttcaaa
      541 aacaatacaa agtgatgaat tggacggtat acgtttaatt acaatacaca aaagcaaagg
      601 acttgagtat gataatgtaa tagtgccttt ttgcgactgg aaccttgata gcagagatac
      661 aatatggtgt gaaccaaata tagaaccatt ctcacaactc cctattgttc ccattgattg
      721 tagtgcaaaa aacatggtgg gtactatata tgaaaaagat tatattaacg aaagccttca
      781 aaacactgta gacaatctaa atttattata tgtagctttc acaagagcta aaaacaatct
      841 tttcgtaatt ggtaaaaaag atacatatgc taataatcgt tctcaactta ttcaagccac
      901 tttagaaagt ctaattaata gtatgcctgg ttgtaccatt aaaaataatt ataatggtga
      961 taatctattg tttgaatacg gttctatatg tataaaagaa atgatagttg gcaatacttc
     1021 aaaaaatgtt tttttgcaag aaaccaaacc agaaagtata gaaattaagt catttgataa
     1081 tcctgtcgga ttcagacaaa gtaataaaag tattgaattt atcgaaaatg aagaagatga
     1141 tgaagaattt tcacaaaaca attatattca gataggtaat attctacacc atttattttc
     1201 aacaatacga aatattaatg atatagatcc agtgcttcat caatttcaat cagaaggttt
     1261 gctttacgat gagagtatta caaaagacaa attatctaaa atgataggtc ttagacttaa
     1321 agataaacgt gtagaagatt ggttctcgcc acactggact gtttttaatg aatgttctat
     1381 aataagtgtg gataaagaaa ccaacgaaat aattgaaaaa cgtccagata gagtaataac
     1441 agatggtaaa cagattattg tgattgattt caagttcggt aaacagaatt ataaatacca
     1501 atatcaagta aaagaatata tgaatcttct tgttggcatg ggatacaaaa gtatcaaagg
     1561 atatatttgg tacgtatata gtaatcaaat aaacgaaata gagttatgaa aaaaacattt
     1621 ttgcaatata cagctgaaag tcttttagaa aaattcaatt gcgatttgtc tggggtaaca
     1681 gtagtttttc caaacaagag agcctcacta ttctttaatg aatatttagc gagagccgct
     1741 aaacgcccta tttggagccc atcttatatt actatcagtg aactatttcg tcaacattct
     1801 aaacttacgg ttggcgaccc tattaaatta atttgtgatt tacacaagag ctttgtacgc
     1861 caaacaggaa ttgatgaaac tttagataga ttctatggct ggggtcaatt acttttggct
     1921 gattttgatg atatagataa aaatatggcc gatgccgaca aggtcttcca gaatctatct
     1981 gatta
//
LOCUS       DAOMTA010000621         1981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2856_length_1981_cov_1.812565, whole genome shotgun sequence.
ACCESSION   DAOMTA010000621 DAOMTA010000000
VERSION     DAOMTA010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_1981_cov_1.812565"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLK68_13670"
     CDS             complement(<1..423)
                     /locus_tag="PLK68_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91495.1"
                     /translation="MNFPKNFLWGGAIAANQAEGAYDEDGKGLNVTDVSTGLINVPDY
                     RVIPGKYYPSHEAIDFYHRYKEDLQLMSEMGFNCFRTSISWGRIFPNGDDEEPNEAGL
                     NYYEEMFNYMLELGMEPVITISHYETPIHLVEQYGGWKS"
     gene            complement(828..>1242)
                     /locus_tag="PLK68_13675"
     CDS             complement(828..>1242)
                     /locus_tag="PLK68_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoylphosphate synthase large subunit"
                     /protein_id="HRM91496.1"
                     /translation="FRLDSDKEGLGKKGDLVGLEVNMRAPGAYMPDMINFSYESDVYT
                     IWADMVIYDKCFIELKQKYLVAYIGRRNDIGYMFDHDQLISQFGGNIMLDVDVPEVLS
                     AAMGNHVYMVRTENQEHLDYLINEFLKRCDGSNWR"
     assembly_gap    1243..1257
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1258..>1981)
                     /locus_tag="PLK68_13680"
     CDS             complement(<1258..>1981)
                     /locus_tag="PLK68_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRM91497.1"
                     /translation="HNFCSRLKNRGVNVLGIGDAQYSEISNETKESLSEYYRVNSLEN
                     YDEVYKAVAYYISKYGRIDFVESQNEYWLETDARIRSDFNICSGTKFEDLAVMKYKSK
                     MKAVYESVGLNVARYCLIDNFENALNFIDAVGYPVVVKPDNGVGATSTYKLNNQAELE
                     YFFATKDERIYIMEEYVNGHVETFDGITDSNKNVLIANSTIMLNSIMDNVNEHCDTAF
                     CNRFVAGSDIEEIGTKVVKAFDT"
BASE COUNT          673 a          407 c          224 g          662 t
ORIGIN      
        1 agatttccag ccaccatatt gttctactaa atgtattggt gtttcataat gtgaaatcgt
       61 tataactggt tccattccca gctccagcat ataattaaac atttcctcat agtaatttaa
      121 ccctgcttca tttggctctt catcatcacc attaggaaaa atacgccccc atgaaatact
      181 tgttctaaaa caattaaatc ccatctcact catcaattgc aaatcttctt tataccgatg
      241 ataaaaatca atggcctcat gactaggata atatttacca ggaataacac gatagtcagg
      301 tacattgatt aatcctgtac taacgtcagt aacattaagc cctttcccat cttcatcata
      361 agccccttca gcttgattgg cagcaattgc accaccccat aaaaaattct ttggaaagtt
      421 cattgtataa attcctcctt gatattacta tcttagcatt atcaatttta aaaattattc
      481 tttttcatat ttataaaagg cttttcaaaa tatgaaaaag tgtcacggtt tatatgggag
      541 aggggccgtg acactataca tagtattata tcagtcttaa aaaaagttgt ctagactatt
      601 taaataaaaa ggagttagga ctgggatgtc ctaactttgt ggggagggaa gaataaattc
      661 ttccttcatt ttgtgcttat gcacatttgg gataaagtgg ttcattactg aaccgccctt
      721 ataatagcat ataaatttca aaaaggaaat ttattttaac acaaatttaa caaatattga
      781 taatatggta atatttttaa cttgaacacc ttaaaaatgt atttaaatta tcgccaatta
      841 ctaccatcac atcgcttcaa aaattcatta attaaatagt ctaaatgctc ttgattttct
      901 gttctaacca tgtaaacatg attacccatc gctgcactta aaacttcagg gacatcaaca
      961 tcaagcataa tatttccacc gaactgacta attaactggt catgatcaaa catataacca
     1021 atatcattac gacgaccaat atatgcaact agatatttct gctttagttc tataaaacat
     1081 ttatcataaa ttaccatatc agcccaaatt gtataaacgt cactttcata actgaaattt
     1141 atcatatctg gcatataagc ccccggagct cgcatattga cttctaaacc aactaaatca
     1201 ccttttttcc ctaaaccttc tttatctgaa tcaagtctga aannnnnnnn nnnnnnntgt
     1261 atcaaaagct ttaactactt tcgttccaat ttcttctata tcactaccag caacaaaacg
     1321 attacaaaaa gcagtgtcac aatgctcatt tacattatcc ataattgaat ttaacataat
     1381 cgtgctattg gcgattaaaa catttttatt actgtcggta attccgtcaa aagtttcaac
     1441 atgtccgttg acatattctt ccataatata aatacgctca tctttagtag caaaaaaata
     1501 ttctaattct gcttgattat tcagtttata agtagatgta gctccaaccc cattatccgg
     1561 ctttaccaca actggatacc cgaccgcatc aataaaattc aaagcatttt caaaattatc
     1621 aattagacaa tatcgtgcca cattcaaacc aacactttca taaacggctt tcatctttga
     1681 tttgtatttc atcactgcca aatcttcaaa tttagtacca ctgcatatat taaaatcact
     1741 gcgaattcgt gcatcagtct ccaaccaata ttcattttga ctttcaacaa aatcaattcg
     1801 accatactta ctaatataat aagctaccgc tttatatact tcatcataat tttccaaact
     1861 attaacacga taatactcac ttaaactttc ttttgtttca ttgctaatct cactatattg
     1921 agcatctcca attcctaaaa catttactcc acggtttttt aatcgagaac aaaagttatg
     1981 a
//
LOCUS       DAOMTA010000622         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2860_length_1980_cov_1.842078, whole genome shotgun sequence.
ACCESSION   DAOMTA010000622 DAOMTA010000000
VERSION     DAOMTA010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_1980_cov_1.842078"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLK68_13685"
     CDS             <1..521
                     /locus_tag="PLK68_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM91498.1"
                     /translation="KGEYMFVNNVYDNYINFNGAPFNVIEYESRGGNYKLCEIDDLAA
                     FADFEINKILTTSDPEYLQEHYQEMMEPFKDSLSCMFTGPFYFEFTAQGIDKAKALDT
                     VLKPMGYSQDEMIAFGDGHNDASMVKYAGTGVAMANAVQDLKDISQYITLSNDEDGIA
                     EALYKYIPELKD"
     gene            640..1029
                     /locus_tag="PLK68_13690"
     CDS             640..1029
                     /locus_tag="PLK68_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRM91499.1"
                     /translation="MPKTRYSKQRELIYENLKARCDHPTAEAIYSDLKKDNPGLSLGT
                     VYRNLNFLADAKMIRKLDVGQTIVHFDGDISDHYHFICNECNQVFDITYHDENISNVV
                     QEHTKHHIEKTDIVFSGICEECLKKKN"
     gene            1054..1638
                     /locus_tag="PLK68_13695"
     CDS             1054..1638
                     /locus_tag="PLK68_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRM91500.1"
                     /translation="MTLKGTKTANNLMHAFAGESQARNRYTYYASIAKKEGFVQIQNI
                     FLETANQEKEHAKRLMKLMNKDLAGETLYTDGNFPVLLGTTAENLKAAAAGENEEYTD
                     MYPSFAKTAREEGFEDIATVLDSIAIAEKHHEERYLALLAALEAGNTFKRETPVVWKC
                     NNCGFIFEGLEAPERCPACDHPQAHFEVNTFFLG"
BASE COUNT          741 a          274 c          323 g          642 t
ORIGIN      
        1 acaaaggtga atatatgttt gtcaacaatg tttatgataa ttatattaat ttcaatggtg
       61 caccattcaa tgttattgaa tatgaatcac gtggtggaaa ctataagtta tgtgagattg
      121 acgatttagc agcttttgct gattttgaga tcaataaaat cttaactact agtgatcctg
      181 aatatcttca agaacactat caagaaatga tggaaccatt taaagatagt ctaagctgta
      241 tgtttaccgg tcctttctac tttgaattta cggctcaagg aattgataaa gctaaagctt
      301 tagatactgt tttaaaacca atgggttata gccaagatga aatgattgct tttggtgatg
      361 gtcataatga tgcatcaatg gttaaatatg caggaacagg tgttgctatg gctaatgccg
      421 ttcaagactt aaaagatatt agtcaatata taactttatc taatgatgaa gatggtattg
      481 ctgaagcatt atataaatac attccagaat taaaagatta gtacgctgaa gcgtactttt
      541 ttcttttatt aataataatt atcaataagc tattgacata tactaacaaa agatatacta
      601 taatagtaac cattactatt atagaaaagg aggtagcgta tgccaaagac aagatactct
      661 aaacagcgtg aattgattta cgaaaattta aaagcaagat gcgatcatcc tactgctgaa
      721 gctatttata gtgatttaaa aaaagataac cccggcttaa gtttaggaac tgtttatcgt
      781 aatcttaact ttttagctga tgctaaaatg atcagaaaat tagatgttgg tcaaacaatc
      841 gtccatttcg acggggacat tagtgatcat tatcatttta tctgtaatga atgtaatcaa
      901 gtgtttgaca tcacttacca tgatgaaaac attagtaatg tagtacaaga acatactaaa
      961 catcatatcg aaaaaacgga tattgttttt agcggtatat gtgaagaatg tttaaaaaag
     1021 aaaaattaaa gaaaataacg gaggaaacta actatgacac ttaaaggaac taaaacagct
     1081 aacaacttaa tgcatgcatt tgcgggagaa agccaagcac gtaatcgcta tacttactat
     1141 gcttcaatcg ctaaaaaaga aggatttgta caaattcaaa atattttctt agaaacagca
     1201 aatcaagaaa aagaacatgc caaaagatta atgaaattaa tgaataaaga tttagccgga
     1261 gaaacattat atactgatgg aaacttccca gtattattag gaactactgc tgaaaactta
     1321 aaagcagctg ctgcaggaga aaacgaagaa tatacagata tgtatccaag ttttgctaaa
     1381 acagctcgtg aagaaggatt tgaagatatt gctacagttt tagacagcat tgcaattgct
     1441 gaaaaacatc atgaagaaag atatttagct ttattagcag ctttagaagc tggtaataca
     1501 tttaaaagag aaacaccagt tgtatggaaa tgtaataact gtggatttat tttcgaaggt
     1561 ttagaagcac cagaaagatg tccagcttgt gatcatcctc aagctcactt tgaagtaaat
     1621 actttctttt taggataatt atttaaagcc cttactagag ggctttttat tattaatatt
     1681 aaaaaactgc tttgaatagt ttcttttgta cagtctaact gctatctata taaatcaact
     1741 attttagtga ttaaaatata attgaaaact aaatttgatg atagcaatta attcttttat
     1801 atttttcatt cttgccacct cctacactta tataataatc tattttaata gtacaaacca
     1861 tcgaacttca tgacaatatt cttacaattt tgtaatattt aattttatga tataatgact
     1921 agcgaggtgc gacaatgaaa ctaattatct tatggctcat taatacaatt tttgttttac
//
LOCUS       DAOMTA010000623         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2861_length_1979_cov_2.059771, whole genome shotgun sequence.
ACCESSION   DAOMTA010000623 DAOMTA010000000
VERSION     DAOMTA010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2861_length_1979_cov_2.059771"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLK68_13700"
     CDS             complement(<1..492)
                     /locus_tag="PLK68_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRM91501.1"
                     /translation="MANLKVSLPGLEMKNPIIPASGTFGFGYEFAKFYDINILGSMML
                     KGTTLEPRYGNPLPRIAEGPSGLLNAIGLQNPGVDAVMAEELEKLRPLYNDKVIANIG
                     GSAPDDYVETAKRISTHDMVGALELNISCPNVHSGGIQFGTNPEMAADLTRRVKEVSK
                     VPVY"
     gene            complement(485..1240)
                     /locus_tag="PLK68_13705"
     CDS             complement(485..1240)
                     /locus_tag="PLK68_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRM91502.1"
                     /translation="MANLHGMMKITSIKNIADDVYEMILEGEGAKYISAPGQFINIKI
                     NDSLQPYLRRPMSISDYDDSHIVIVFKVVGEGTKILKNKEIGSYLDCLIGLGNGFTIK
                     DGKALLIGGGLGTPPLYNLGKKLAAKGIEITTVLGFGSAKDVFYQEKFAEFSNVFVAT
                     MDGSTGTKGTVVDVIKNEGLTFDNYYTCGPEPMLDALALNYPENGQLSFEARMGCGFG
                     ACMGCSCKVKTRPYKRICVEGPILESNEVIVNG"
     gene            complement(1249..>1979)
                     /locus_tag="PLK68_13710"
     CDS             complement(1249..>1979)
                     /locus_tag="PLK68_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM91503.1"
                     /translation="DEAELQPGACINDGNYAKEHNLVGINNASEFNHAVRDLKLASEI
                     GNRYHICHISTKETVAALTEARKTNPLVSGEACPHHLILTDDNIKDMNPNYKMNPPLR
                     SKEDLAALIQGINNGGVTVISTDHAPHAIEEKNKPIDKAPFGIIGNQHAFSLMYTYLV
                     KKGLISLEKVLTCMSINPAKVIGIEHDLEVGLKANLAVFDLEEEYVITKESIKSKSIN
                     TPFLETKCFGALKYHVLDGKVTKI"
BASE COUNT          608 a          410 c          264 g          697 t
ORIGIN      
        1 ataaactgga actttagaaa cttccttaac tcgacgtgtt aaatcagctg ccatttctgg
       61 attagttcca aattgaattc caccactatg gacattcggg catgaaatat tcaattctaa
      121 ggctcctacc atgtcatgag tagaaatcct tttagcagtt tcgacataat catcaggtgc
      181 actaccacca atattagcaa ttactttatc attatatagc ggtcttagtt tttctaattc
      241 ctcagccata acagcatcaa caccaggatt ttgcaaacca attgcattta acaaaccact
      301 tggtccttca gcaattcgag gtagagggtt cccatagcga ggttctaacg tagttccttt
      361 aagcatcatt gaaccaagaa tattgatatc ataaaacttt gcgaactcat atccaaaccc
      421 aaatgttcca ctcgctggaa taatgggatt tttcatttct aaaccaggta aagatacttt
      481 taaattagcc atttacaatc acctcgttac tttctaagat tggtccttca acgcagattc
      541 ttttatatgg tctagtttta actttgcaag agcatcccat gcaggcacca aagccacatc
      601 ccatccgagc ttcaaatgat aattgcccat tttctggata attaagtgct aaagcatcta
      661 gcattggttc tggtccacaa gtataataat tatcaaaagt caaaccttca tttttaatta
      721 catcaacaac cgttcctttt gttccggtgc tgccatccat tgttgcgaca aaaacattag
      781 aaaattcagc aaatttttct tgatagaaaa catcttttgc actgccaaaa cctagtactg
      841 tcgttatctc tattcctttg gctgcaagct ttttacctaa attatatagg ggtggtgttc
      901 ctaatccacc accaatcaat aaagctttac catccttaat ggtaaatcca ttacctaacc
      961 caatcaaaca atctaaatag ctgccgattt ctttattttt taagatttta gttccttctc
     1021 caactacttt aaaaacaatc acaatatgac tatcatcata atcactgatt gacatcggtc
     1081 ttcttaaata aggttgtaaa ctatcattga ttttaatatt gataaattgg cccggagcac
     1141 tgatatattt agctccttca ccttctaaaa tcatttcata tacatcatct gcaatatttt
     1201 taatgcttgt aatcttcatc atcccatgta agtttgccat aatgcctcct atatttttgt
     1261 aactttacca tctaaaacat gatactttaa tgctccaaaa cacttagttt caagaaatgg
     1321 tgtattaatt gattttgatt taatactttc ttttgtaatt acatattctt cttctaaatc
     1381 aaaaacagca agatttgcct ttaacccaac ttctagatca tgctcgattc caattacctt
     1441 tgctggattt atagacatac atgtcaaaac tttttctaaa gaaataagtc cttttttaac
     1501 taaatatgta tacattaagc taaatgcatg ttgattccca ataattccaa atggagcttt
     1561 gtcgattggt ttattttttt cttcaatcgc atgtggtgca tgatcagtag aaattacagt
     1621 tacaccccca ttattgattc cttgaatcaa tgccgctaaa tcttctttac ttcgaagcgg
     1681 tggattcatt ttataattag gattcatatc cttaatatta tcatcagtta aaattaaatg
     1741 gtgtggacat gcttcaccgc taactaatgg atttgttttc cgagcttcgg ttaatgccgc
     1801 tactgtttct ttagttgata tatgacaaat atgataacga tttccgattt cacttgccaa
     1861 ctttaaatca cgaacagcat gattaaattc agatgcatta tttattccaa ctaaattatg
     1921 ttcttttgca taattaccat cgttgataca agcacctggc tgtaattcag cttcgtctt
//
LOCUS       DAOMTA010000624         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2869_length_1975_cov_3.226562, whole genome shotgun sequence.
ACCESSION   DAOMTA010000624 DAOMTA010000000
VERSION     DAOMTA010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2869_length_1975_cov_3.226562"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLK68_13715"
     CDS             <1..752
                     /locus_tag="PLK68_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM91504.1"
                     /translation="KNKKVLGVIHLKDIIKQGVKEKFADLRKMGIKTVMITGDNPLTA
                     AAIAAEAGVDDFLAEATPEGKLEMIRELQAKGHLVAMTGDGTNDAPALAQADVAVAMN
                     TGTQAAKEAGNMVDLDSSPTKLIDIVRIGKQLLMTRGSLTTFSIANDVAKYFAIIPVL
                     FITLYPGLDALNIMGLHSKDSAIFSAIIYNALIIIALIPLALKGVKYREVSAGKLLSR
                     NLLVYGLGGIIAPFICIKIIDVIIVALHIVS"
     gene            766..1371
                     /gene="kdpC"
                     /locus_tag="PLK68_13720"
     CDS             766..1371
                     /gene="kdpC"
                     /locus_tag="PLK68_13720"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HRM91505.1"
                     /translation="MKHLKTSVLPALKIFLIFTVVCGVIYTAAITGFAQIVFPDKANG
                     SIIEVDGKKYGSELLGQQFIDDTHMWGRIMIIDGETFANKDGEKTMYGLASNSSPASE
                     DYEKVIAERVAMIETANPEQKGKQIPVDLVTVSGSGLDPHISLAAAEYQIPRLVRTTG
                     KSEAEIRKIIDKYTDHGFLGYFGETTVNVLKVNLALDGILK"
     gene            1480..>1975
                     /locus_tag="PLK68_13725"
     CDS             1480..>1975
                     /locus_tag="PLK68_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM91506.1"
                     /translation="MDEQRPDPEELLKQIKQEEIAKQRGKLKIFFGYAAGVGKTYAML
                     EAAHVAYHAGVDVVAGYVEPHQRPETSKLLDGLEVLPPLKVTHNGIMLNEFDLDGALK
                     RNPDLILVDELAHTNDEQCRHLKRYQDINELLDHGIDVYTTINVQHIESLNDIIASIT
                     AVVVK"
BASE COUNT          664 a          275 c          409 g          627 t
ORIGIN      
        1 ctaaaaataa aaaggtttta ggagtaattc atcttaaaga tattattaaa caaggggtta
       61 aagaaaagtt tgctgatctt cgtaaaatgg gaattaaaac ggttatgata accggtgata
      121 atcctttaac agcagctgca attgcagctg aagctggagt tgatgatttt ttagctgaag
      181 ctacgccaga aggcaaatta gaaatgattc gagaacttca agctaaaggt cacctcgttg
      241 caatgactgg tgatggaact aatgatgctc cagctttggc tcaagcagat gttgcagtag
      301 caatgaatac aggtactcag gcagctaaag aagctggaaa tatggttgac ttagattctt
      361 caccaactaa attgattgat attgttcgta ttgggaaaca attattaatg acaagaggaa
      421 gtttaacaac tttttcaatt gctaacgacg tagctaaata ctttgcaatt attcctgtac
      481 tattcattac tttatatccg ggattagatg ctttgaatat tatgggatta cacagtaagg
      541 atagtgcgat tttctcagca atcatctata atgcattaat tattattgcg ttgattccgt
      601 tagctttaaa aggagtaaaa tatcgtgaag tgtcagctgg taagttatta tcaagaaatt
      661 tattagtata tggattaggt ggaattattg ctccctttat ttgtattaaa attattgatg
      721 taattattgt tgctttgcat attgtatcgt aggaggagaa gtttaatgaa acatcttaaa
      781 acaagcgttc tacccgcttt aaaaatattt ctgatcttta cagttgtttg tggagttatt
      841 tatactgctg ctattactgg ttttgctcaa attgtttttc ctgacaaagc aaatggaagc
      901 attattgaag tagatggaaa aaaatatggt agtgaattat taggtcaaca atttatcgat
      961 gatactcata tgtggggtag aatcatgata attgatggtg aaactttcgc aaataaagat
     1021 ggtgaaaaga caatgtatgg tctagcatct aacagttctc ctgctagtga agattatgaa
     1081 aaggttattg ctgaaagggt tgcaatgatt gagactgcaa atccggagca gaaaggtaaa
     1141 caaatacctg ttgatctagt aactgtatct ggttctggac ttgatccaca tatttctcta
     1201 gcagctgcgg aatatcagat tccacgttta gtaagaacaa caggaaagag tgaagctgaa
     1261 attagaaaga ttattgataa atatactgat catggatttc ttggttattt tggagaaaca
     1321 acagtaaatg tccttaaagt caatttggct cttgatggca ttttgaaata aaaataagtg
     1381 tctgtcatta tgataggcac ttattttaac aaagcaatct taatactttt ttaatatcct
     1441 aggcgctata ataaaaataa cagtaaagga ggaagtgtaa tggatgaaca aagaccagat
     1501 cccgaagaat tgttaaaaca gattaaacaa gaagagattg ctaaacagcg cggtaaatta
     1561 aaaatatttt ttggctacgc agcgggtgtt ggaaagactt atgccatgtt ggaagcagct
     1621 catgttgctt atcatgccgg tgtcgatgta gtagcaggct atgttgagcc gcatcagcgt
     1681 ccggaaacat caaaattatt ggacggatta gaggtcttac ctcctttaaa agttactcat
     1741 aacggaatca tgctcaatga gtttgatttg gatggtgcat taaaacgaaa tcccgattta
     1801 atattggttg atgaactggc tcatacaaat gatgagcagt gtcggcattt aaaacgatat
     1861 caggatatta atgaattact agatcatggc atcgacgttt atacaacgat aaatgttcaa
     1921 catattgaga gtttaaacga tattattgct tcaattactg cagttgtggt caaag
//
LOCUS       DAOMTA010000625         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2870_length_1975_cov_2.110937, whole genome shotgun sequence.
ACCESSION   DAOMTA010000625 DAOMTA010000000
VERSION     DAOMTA010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2870_length_1975_cov_2.110937"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    388..397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(599..>1071)
                     /locus_tag="PLK68_13730"
     CDS             complement(599..>1071)
                     /locus_tag="PLK68_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HRM91507.1"
                     /translation="TFNLAGMIGSYHIVYNKWLKDRLDKEESLTHYNSMNVLSMHALI
                     GAYTKGNEWLGQLKDILEENSKYAINYINEHFEGVFVSQTQGTYLIFLQCHDWCQKHH
                     LSNDELYLKGLEVGVMWTNGKEYKDNYGMRMNLSLPLSRLKEALDRLDKYVFNG"
     assembly_gap    1072..1081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1082..1868)
                     /locus_tag="PLK68_13735"
     CDS             complement(<1082..1868)
                     /locus_tag="PLK68_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM91508.1"
                     /translation="MKYDFTTVLDREGHDSLAYDAPVHGTMNIFPDYKLQEGFDVIPM
                     WIADMSFAVPDFIIENVKKRLEFPVYGYFAYSKEYYQAIIDWQKQQHQVNDLLPENIG
                     YENGVIGGVVNALNILCSKGDKFLIHSPTYNGFSNVISPNGYEMVLSPLIKDNDGVWR
                     MDYEDMERKIVENNLHVALLCSPHNPTGRVWEKEELLKAIEIFERHQVYVISDEIWSD
                     LIFKGYHHTPTQNVNDYAKNHTISFYAPSKTFNLASMMISNIFI"
BASE COUNT          673 a          330 c          204 g          748 t
ORIGIN      
        1 attttgaatg ttttaatcaa tgaaaatgtc tgtatttgca aaaattttca caatctaata
       61 ctctccgata cccaaatttc aaatactatt ttcttatatc tatactaaat attatgttat
      121 tataactata acatagttta tattttatta tgataattat ttattacact tacagaactg
      181 caattttaaa tattttatat ccaactatat taagtaatct ttaaaaaatt aattttcatc
      241 aaagcatatt aatatataat atttcttttt tgtatttacc ttctttttat attttaccga
      301 catatacatt atattgtctt ctaagtaatt ccattgttac tttatttccg taagctctat
      361 aataattcgt atttttttct caatattnnn nnnnnnngct gcaacaaaaa gcatagaaaa
      421 aatcactttt taaagaaaca attgagtaaa tatccaactg ttcctgtttt ttactttcct
      481 gtacaatatt aacttttagt ctaaataatt gaatttactt cactttatat tattttttta
      541 tataaaagct acaaatttac tttaatactt cataatctca tttaaagtga tattagtatt
      601 atccattaaa tacatattta tccaatctat ctagtgcttc ttttaatcta gacaaaggta
      661 gcgataaatt catgcgcatt ccataattat ctttatattc tttaccatta gtccacatta
      721 caccaacttc taatcctttt agatatagtt catcattact tagatgatgc ttttgacacc
      781 agtcatggca ttgaagaaaa attaaatatg ttccttgagt ttgactaaca aaaacacctt
      841 caaaatgttc attaatataa ttaatagcgt atttagagtt ttcttccaat atgtctttta
      901 attgccctaa ccactcatta cctttagtat acgctccgat caatgcatgc attgatagta
      961 cattcatgga attataatgt gttaagcttt cctctttatc tagacgatct tttaaccatt
     1021 tattataaac gatatgataa ctacctatca ttccagcaag attgaaagtt tnnnnnnnnn
     1081 ngaataaaaa tattggaaat catcatactt gcaagattga aagttttact tggggcataa
     1141 aaggatattg tatgattttt tgcataatcg ttaacattct gtgttggtgt atgatgatag
     1201 cctttaaaaa taagatcact ccaaatttca tcagatatta catatacttg atgtctttca
     1261 aatatctcta ttgctttaag caattcctct ttttcccata ctctaccagt aggattgtgg
     1321 ggtgaacaaa gtaaggcaac atgaagattg ttttctacaa tttttctttc catatcttca
     1381 taatccattc tccatactcc atcattatct ttaattaatg gacttaaaac catttcataa
     1441 ccattaggac taataacatt agaaaaacca ttataagtgg gagagtgaat taaaaactta
     1501 tcacctttag aacataagat atttaaagca ttaacaaccc cgccaataac tccattttca
     1561 taaccaatat tttctggtaa taaatcatta acttgatgtt gttgtttttg ccaatcaata
     1621 atagcctgat aatattcttt actataggca aagtatccat aaactggaaa ctctaaacgt
     1681 tttttaacat tttcgataat aaagtctggt accgcaaaac tcatatctgc aatccacatt
     1741 gggatgacat caaatccttc ttgtaactta taatcaggaa agatattcat tgttccatgt
     1801 actggagcat cataggctaa agaatcatgt ccttcacggt ctaagacagt tgtaaaatca
     1861 tacttcataa taataaacct ttctattttt atattataaa actatatgct tttaccctac
     1921 agaaccttct agcgaaactg aaagtaattt acgtgcctca acagcaaatt ccata
//
LOCUS       DAOMTA010000626         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2874_length_1974_cov_1.895258, whole genome shotgun sequence.
ACCESSION   DAOMTA010000626 DAOMTA010000000
VERSION     DAOMTA010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_1974_cov_1.895258"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..>1009
                     /locus_tag="PLK68_13740"
     CDS             263..>1009
                     /locus_tag="PLK68_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91509.1"
                     /translation="MFKFIEPKIQLDKKRQIFTNKHLRALIIPLIIEQLLILLVGIAD
                     TLMVSYAGEAAVSGVSLVNQLNTVFILVFTALASGGAVVASQYIGNKNKENGVLAASQ
                     LVMITTIISVILLVLSVIFREQLLTLLFGKVAPDVMDACLKYLVISAFSFPALAIYNS
                     CAGLFRSMSKTSVIMYVSIIMNIINIIGNAIGIFILHAGVAGVAYPSLISRVVAALIL
                     LIISFNQKNIIYIRIKDIFKWNAQMIKRILN"
     assembly_gap    1010..1019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1020..1642
                     /locus_tag="PLK68_13745"
     CDS             <1020..1642
                     /locus_tag="PLK68_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91510.1"
                     /translation="IPNGIENGLFQLSKVALSSIVAMFGTSQIAANGVAQSFWSMAAL
                     FCIAMGPAFITVIGQCMGAGDKEAADYYMKKLLRITYIGGIIWNIVFFLFTPIILKLY
                     SLSNETIKLIIILCFIHNLFNALLCPIASSLSNGLRAAGDVKYTMYTSIFSTVVCRVI
                     LSFIFGVWMNLGVIGIALAMVGDWAIKSALILVRYLQGQWKNFKVI"
BASE COUNT          630 a          287 c          322 g          725 t
ORIGIN      
        1 cgttattttg aagcaaaaag aattattggt aaaaatattg cattatcacc tcaatgtggc
       61 ttcgcctctg ttgaagaggg aaactgtatt gatgaaaaaa ctcaatgggc aaaaatagac
      121 cttttagttt catgtcagga ttttttataa atcttattag agaaacaatt agtttctctt
      181 ttttattttc ttcattatat aaaaaaacaa ttgacttaga gtcaagtcga ggttgtatta
      241 tatgaaaaac ggaggaatat atatgtttaa atttattgaa ccaaaaatac aattagataa
      301 aaaacggcaa atctttacaa ataagcatct acgagctcta atcatcccct tgatcattga
      361 acaattattg atcttattag ttgggatagc tgatactcta atggttagtt atgctggtga
      421 agcagccgta tctggtgtct cactagtcaa ccagttgaat acggtcttca ttcttgtctt
      481 tactgcatta gcatctggtg gtgccgttgt tgccagccaa tatatcggta ataaaaacaa
      541 ggaaaatgga gtgttagcag ctagtcaatt agttatgata acaacaataa tttcagttat
      601 tttattagta ttatcggtta tttttcgaga acaattacta acattattgt ttggaaaagt
      661 cgctccagat gtaatggatg cctgcttaaa atatttagtt atttctgctt tttcttttcc
      721 tgctttagca atctataaca gttgtgccgg attgtttaga agtatgtcta aaacaagtgt
      781 tatcatgtat gtatcaatca tcatgaatat tattaatatt attggtaatg ctattggaat
      841 ttttattctt catgcaggtg ttgcaggagt cgcataccct tcactaattt ctcgagtcgt
      901 tgcggcttta attttattaa ttatatcttt taatcagaaa aatattattt atattcgcat
      961 taaggatatt tttaaatgga atgctcaaat gattaagcgt atcctaaatn nnnnnnnnnc
     1021 tattcccaac ggcattgaaa atggcttatt tcaattatct aaagttgctt taagtagtat
     1081 tgtcgcaatg tttggaacta gccagattgc tgcaaacggg gttgcacaaa gcttttggtc
     1141 aatggccgct cttttttgta ttgcaatggg accagctttt atcactgtta tcggtcaatg
     1201 tatgggagcc ggtgataaag aagcggcgga ctattatatg aaaaaattat taagaatcac
     1261 ttatattggc gggatcattt ggaatattgt atttttccta tttaccccta tcattcttaa
     1321 attgtatagt ttatcgaacg aaacgattaa actaattatt attttatgtt ttattcataa
     1381 cctatttaat gccttattat gccctatagc ctcatcatta tcaaatggcc ttagagcagc
     1441 tggtgacgta aaatacacga tgtatacttc aattttttca actgttgtgt gtcgtgtcat
     1501 cttatcattt atttttgggg tttggatgaa ccttggtgta attggaattg cattagcaat
     1561 ggttggcgat tgggctatta aatctgctct tatcttagta cgttatttac aagggcaatg
     1621 gaaaaatttt aaagtcatct aattatcttg actatttatt tttaaacaag ctgattattt
     1681 ttcattttta tctgtgctat aattaaacag aggagtctag ttataatgaa aaaaataata
     1741 aaacctattc aaccaataaa accaagccca ccaacaaaaa acaaaccaaa aaattcttca
     1801 attaaaaagc catctaaata aatggctttt tattattata aaaattctct ttttatttca
     1861 gaagaaatat ttttaattac tttagatgta aaaggattac tgaaacctga gtcttttaat
     1921 gcatctaagt aatcaacatt tagagtgttt tgagtcatat tgcgatatgc attt
//
LOCUS       DAOMTA010000627         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2876_length_1973_cov_2.218978, whole genome shotgun sequence.
ACCESSION   DAOMTA010000627 DAOMTA010000000
VERSION     DAOMTA010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_1973_cov_2.218978"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLK68_13750"
     CDS             complement(<1..725)
                     /locus_tag="PLK68_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRM91511.1"
                     /translation="MPVYSIKTIKEYANNYISNNGREITKTVLLITSAVIFVQAVNFF
                     SQVLGLILGLITLTMPQGLVYASLKIVDQQELSAIDDTLYGIKKISKYFTTYFIYSLI
                     AVFVLALVIVAFILLVSYCDIGIIGGRDIVSILAQYWIIVITLLVIVIGTYVFLDCNY
                     GMYPYLMETSDVKNLRALKRSRQFMKHKKKAYLKLYLSFWKEYLGLIIITLLVSFVLP
                     TMLDISAIIFIVGIALNFNILIN"
     gene            complement(968..1684)
                     /locus_tag="PLK68_13755"
     CDS             complement(968..1684)
                     /locus_tag="PLK68_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM91512.1"
                     /translation="MITSTTNSTIKTLMKLKQKKYRDEMKAYLVEGDHLVTEALKANQ
                     VELLISTKHIDSELEVLEVSEEVMAKLAFTKSPQSIMAKCRMQKEIQLKEGRRYLLLD
                     DLQDPGNIGTLIRTALAFSIDQVILSNNCVDLYNDKLLRSMQGANFHLSCIYGNLTQL
                     ISQLQEKGVVVIGSALENGKNIAQINRYSKMAFVVGNEGNGMNPEVLAKCDDIGYIPI
                     NTIESLNVAIAGSIMMYHFK"
     gene            complement(1681..>1973)
                     /locus_tag="PLK68_13760"
     CDS             complement(1681..>1973)
                     /locus_tag="PLK68_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRM91513.1"
                     /translation="LEFKIKEYLKSNFSLNMPVIQIKKLKARWGACFSNQNKVCFNLV
                     LVHLEKELIDYVIVHELCHFIQPNHSKLFYQEVQKRLPDYKEREKKLKEIGI"
BASE COUNT          700 a          350 c          218 g          705 t
ORIGIN      
        1 aaattaatta aaatattaaa atttaacgca ataccaacaa taaaaataat tgcactaata
       61 tccagcattg taggtaatac aaacgaaact agtaatgtaa ttataatcaa tcctaaatac
      121 tctttccaaa aacttaaata taattttaga taggccttct ttttatgctt cataaattgt
      181 cgtgaacgtt ttagtgccct tagatttttt acatcacttg tttccattaa atatggatac
      241 attccataat tgcaatcaag aaaaacatat gttccgatga caattactaa taaagtaatt
      301 actattatcc aatactgtgc taagattgaa acaatgtccc tgccgccaat aattccaata
      361 tcacaatatg atacaagtaa tataaatgca actataacta gagctaaaac aaaaacagca
      421 atcagtgaat aaataaaata agtcgtaaaa tatttactga tcttctttat tccgtaaagt
      481 gtatcatcaa ttgcagataa ttcttgctga tcaacaattt ttaatgatgc gtaaactaac
      541 ccttgtggca ttgttaatgt aattaatcct aaaataagcc ctagcacttg actaaaaaaa
      601 ttaacggcct gaacaaaaat gacagctgat gtaatcagaa gaactgtctt cgttatctct
      661 cgaccgttat tactaatata attattagca tattctttta ttgttttgat tgaatacact
      721 ggcatataaa tcccctccct attcatttaa ttgccctgat caactcttta attgtttaat
      781 tttacactgt gttattttaa atatttatct atcttcgtaa aattaaccca atcaataaca
      841 atataatata aacacaatta attatattta ttatttttat gtgttaatat tatctaaatt
      901 aattataact tagataatat aatactgcaa ctaatatata ttataaaaaa cagtcacacc
      961 gtgactgtta cttaaaatga tacatcataa tacttcccgc aatagcaaca tttaatgatt
     1021 caatcgtatt aatcggtatg tatccaatat catcacattt agcaagaact tctggattca
     1081 tcccattacc ttcattacca acaacaaaag ccatcttact atagcgattt atctgtgcaa
     1141 tattctttcc attttcaagt gcactgccaa ttaccacaac acctttttct tgtaattgcg
     1201 aaattaattg tgttagattc ccataaatac aactaaggtg aaaattggcg ccttgcattg
     1261 agcgaagtaa tttatcatta tataaatcaa cacagttatt agataaaata acttgatcaa
     1321 ttgaaaaagc taaagcagta cgaatcaaag taccaatatt tccaggatct tgtaaatcat
     1381 ctaaaagcaa ataacgtctt ccttctttta attgtatttc tttttgcatc cggcacttag
     1441 ccataattga ctgaggagac ttggtaaatg ccagtttagc cattacttcc tcacttactt
     1501 caagtacttc taattcactg tcaatatgtt tggtactaat tagcagttcc acttgatttg
     1561 cttttaatgc ctctgtaaca agatgatcgc cttcaactaa atatgccttc atttcatcac
     1621 gatacttttt ttgttttaat ttcatcaatg ttttaattgt actattagtg gtagaagtta
     1681 tcatattcca atctccttta actttttctc gcgctctttg taatccggga gcctcttttg
     1741 tacttcttga taaaatagct ttgagtgatt aggctgaata aaatgacata gttcatgaac
     1801 aattacataa tcaatcaatt ctttttctag atgtactaga accaaattaa aacaaacctt
     1861 attttgattt gaaaaacacg ctccccaacg agcttttaac ttcttaatct gaattaccgg
     1921 catatttaaa ctaaaattac tttttaaata ttctttaatt ttaaattcta aat
//
LOCUS       DAOMTA010000628         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2878_length_1973_cov_1.840459, whole genome shotgun sequence.
ACCESSION   DAOMTA010000628 DAOMTA010000000
VERSION     DAOMTA010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_1973_cov_1.840459"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    250..259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<260..540)
                     /locus_tag="PLK68_13765"
     CDS             complement(<260..540)
                     /locus_tag="PLK68_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91514.1"
                     /translation="MKYVLLDTNVLIYREGEKVLDKDVQTISRLLMDSDEFRLCIHPL
                     SIKELAKHKDEKQRNVILSKVNIYNELENPPIITEEFNETCGGKNNEHE"
     gene            complement(551..1087)
                     /locus_tag="PLK68_13770"
     CDS             complement(551..1087)
                     /locus_tag="PLK68_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM91515.1"
                     /translation="MSKKIIFIAGSYGVGKTTICNQLSKSLKIKAYSASDLISKQNNE
                     QYGKNKYVKDSDDNQRILVNEVSKIKDSTFILNGHFCLKSLDNNIIVLNQEIFNQMNL
                     SCILLISANPDTIKLNLYNRDCIEYDINFLRRIIASEKKQANEISIKFDIPLYEYEMQ
                     YDGKDVKRILNIFQSSEE"
     gene            complement(1235..1654)
                     /locus_tag="PLK68_13775"
     CDS             complement(1235..1654)
                     /locus_tag="PLK68_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91516.1"
                     /translation="MFLTENELKDKFWKSYNGNNRALRYQFECSIREGNADLVTVEKY
                     QENFQINAFEFKLTDIRKVIRQAEENAKLVNKSWIVIPADRKSVISDKYIGACKEKGI
                     GILYVEDGGRWSLGLSPRYKREIPLTQQLINLMMKGY"
     gene            complement(1692..>1973)
                     /locus_tag="PLK68_13780"
     CDS             complement(1692..>1973)
                     /locus_tag="PLK68_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91517.1"
                     /translation="DDDVKDFYTVDMFLSDLCDLFQDESLLKKAISEYDEFECLLIGE
                     SLITNETLYCILKMNDELENKKNIMRKMLNNSVVFRSYKSLNEFIIDNR"
BASE COUNT          636 a          332 c          174 g          821 t
ORIGIN      
        1 tacattatat aaaaattcat cttgaactat tggaggagtt tttaatgtaa ttaaatttgt
       61 atttttagaa attagagcaa ttgctttaga aattgttaaa actctatctt ctagattaaa
      121 tttttttgac tttttgataa ttcccttatc atttgttatt aaataactta cacaattttt
      181 atatactgaa tataataaat tattatctat taattcaatt tatatggtcg ctagtaattg
      241 gctttttatn nnnnnnnnna attcatgttc attatttttt ccaccacatg tttcattaaa
      301 ttcttctgtt attataggcg gattttctaa ttcattgtaa atattcactt ttgacaaaat
      361 tacattacgc tgtttttcat ctttgtgctt tgctaattct ttaattgata aaggatgaat
      421 gcaaagtcta aactcatctg aatccattaa caatcttgaa atagtttgaa catctttatc
      481 taaaactttt tcaccctcac gatatatcaa aacatttgta tccaataata catatttcat
      541 cattcatcac ctactcttcg ctactttgaa atatatttaa tattcgtttt acatcttttc
      601 catcatactg catttcgtat tcgtaaagtg gaatatcaaa ttttattgaa atttcatttg
      661 cttgtttttt ttcactagca attattcttc taagaaaatt aatatcatat tctatacagt
      721 cacgattata taaattcaac tttatcgtgt caggattagc agatatcaac aatatacaac
      781 ttaaattcat ttgattaaat atttcttgat ttaatacaat aatgttatta tctagcgatt
      841 tcaaacaaaa atgtccatta agaataaatg tactgtcctt tattttagat acttcattaa
      901 ccaatattct ttgattatca tcactatctt taacatattt attttttcca tattgttcat
      961 tattttgttt tgaaattaaa tcacttgcag aataagcctt aatttttaaa ctttttgata
     1021 attgattgca tattgttgtt ttaccaactc cataacttcc agcaataaat ataatttttt
     1081 ttgacacaca tatcactcct cttcccaatc tatacatata attataactt ttttaactat
     1141 cataatctaa tattttaaat aataattata agcaaagtaa ctatttgtat tggaaattag
     1201 ctgatatttg gttttatata tttatttgat gaattcaata tcctttcatc atgagattaa
     1261 ttaactgttg agtaagtgga atttcacgtt tatatctagg tgacagcccc aaactccacc
     1321 gtcccccatc ttcaacatac aaaattccaa tccctttttc tttacacgct cctatatatt
     1381 tatcacttat cacactttta cgatcagcag gaataacaat ccaactcttg ttaacaagtt
     1441 ttgcattttc ttcagcctgt ctaattactt ttcttatatc ggtcaattta aactcaaatg
     1501 cgtttatctg aaagttctcc tgatattttt caactgttac aagatcagca ttgccttctc
     1561 taatactgca ctcaaactga tatctcaacg ctcgattatt tccgttgtat gatttccaaa
     1621 atttatcttt taattcattc tcagttaaaa acattttatt cctccatttt ataatccagg
     1681 tctttgctaa cttatctgtt atctataata aattcattta gacttttata actcctaaaa
     1741 accactgaat tatttaacat ttttctcatt atattttttt tattttctaa ttcatcattc
     1801 attttcaaaa tacaatacag agtctcattg gtaattagcg attcgccaat taataaacat
     1861 tcaaattcat cgtattcgct aattgctttt tttaaaagtg attcatcttg aaagagatcg
     1921 cacaaatcac ttaaaaacat atctaccgta taaaaatctt ttacatcatc atc
//
LOCUS       DAOMTA010000629         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2881_length_1969_cov_1.780564, whole genome shotgun sequence.
ACCESSION   DAOMTA010000629 DAOMTA010000000
VERSION     DAOMTA010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2881_length_1969_cov_1.780564"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..477
                     /locus_tag="PLK68_13785"
     CDS             142..477
                     /locus_tag="PLK68_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRM91518.1"
                     /translation="MLCRDYLYQEVEVLIDRPLGSQHPQYGFIYPLNYGFIENTVSGD
                     GEELDAYLLGVFEPVERYRGVVIAVIQRTNDNDDKLIVVPHNVDYSDEQIRALTEFQE
                     RYFNSVILR"
     gene            489..833
                     /locus_tag="PLK68_13790"
     CDS             489..833
                     /locus_tag="PLK68_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRM91519.1"
                     /translation="MTITVDEYINSFYQQIQINLIKLRKIIKETAPEAQEKISWGVPT
                     YFQDGFLVQFAAYKKHIGFYTSPQTIEAFKTELANYKTNNKNTVQFMFEQELPVELIK
                     KMVLFKIEENTK"
     gene            830..1282
                     /locus_tag="PLK68_13795"
     CDS             830..1282
                     /locus_tag="PLK68_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM91520.1"
                     /translation="MRLGTTYISVNDFAKSLAFYQLLLEQEPLYCNDDRWATFDCGNS
                     LSLYNRQFDIDFLVNNDYRGHYNHSYLTTLKNENSDKINTSVIFNFEVEDLISEYERI
                     KKLKIGAVSELLFVNIHMPYWYFTIKDPDGNEIEITGNYNGSDKLTDE"
     gene            1363..>1969
                     /locus_tag="PLK68_13800"
     CDS             1363..>1969
                     /locus_tag="PLK68_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM91521.1"
                     /translation="MKRKFFFFDIDGTLAVGTPGNQYVPESTKIAISKLKEAGHFVAI
                     ATGRSYAMAVEHMKELGFENMVSDGGNGITIHNKLVEIKPLDYDKCINLINECKEKGY
                     IWAISPDNATRRLAPDNRFYDFTHDIYMDTIVQEGLDPKDFDKIYKVYVACYAPEEEK
                     LETLKELPWCRFHQEYLFVEPGDKSVGIKMMVDHFDGDYSDV"
BASE COUNT          720 a          232 c          376 g          641 t
ORIGIN      
        1 ttaaagattt tattaccaat acgtatagtg atggatcagt gattccggtt aaataaagat
       61 gcttaggcat ctttttttat tatggcgatt gcgtagatac agaaaataac atataatttt
      121 taaagttagg aggttttacc aatgttatgt agagattatt tgtatcaaga agtagaggtt
      181 ctcattgatc gtccattagg ttctcagcat cctcaatacg gcttcatata tcctcttaat
      241 tatggtttca ttgaaaacac agttagcgga gatggagaag aacttgatgc ttatttgctt
      301 ggtgtctttg agccggtaga aagatatcgt ggggtagtta ttgcagttat ccagcggact
      361 aatgataatg atgataaatt gattgttgtt cctcataatg ttgattatag tgatgaacag
      421 attagagcct taacagagtt tcaagagaga tatttcaatt cagttattct aagataaagg
      481 agaagatgat gactataaca gtagacgaat atattaatag tttttaccaa cagatacaaa
      541 taaatttaat taagttaaga aaaataatta aagaaacggc gcctgaagct caagaaaaaa
      601 tatcatgggg tgtaccaaca tattttcaag acggttttct agtccagttt gcagcatata
      661 aaaaacatat cggcttctat acatcaccgc aaacaataga agcttttaaa acagaattag
      721 ctaattataa gacaaataat aaaaatacag tgcaatttat gtttgaacaa gaattaccag
      781 tagaattaat aaaaaagatg gttttattta aaatagagga gaacactaaa tgaggctagg
      841 aacaacatat attagtgtca atgattttgc taagtcactg gctttttatc agcttttatt
      901 ggagcaagag ccattatatt gtaatgatga tcggtgggct acatttgatt gtgggaattc
      961 attatcatta tataatcgac aatttgatat tgattttctt gtaaataacg attatcgtgg
     1021 ccattataat cattcatatt tgacaacttt gaaaaatgaa aatagtgata aaataaatac
     1081 atcagttata ttcaattttg aagtagagga tttaataagt gaatatgaac gaattaaaaa
     1141 attaaagata ggggctgtat ctgaactgtt atttgtcaat atccatatgc catactggta
     1201 ttttacaatc aaagatccag atggtaatga aattgaaatt actggcaatt ataatggaag
     1261 cgacaagcta acagatgagt aaatttttat cttttcatat tcagtaaata aaaaataatt
     1321 atattaaaat atgataggat aaaacagttg gaggttttaa taatgaaacg taaatttttc
     1381 ttttttgata ttgatggtac tttggcagtt gggacaccag gaaatcaata tgtaccagaa
     1441 tcgactaaaa tagctatttc aaaattaaaa gaggctggtc attttgttgc tattgctact
     1501 ggaaggagtt atgcgatggc agtagaacat atgaaagaac tagggtttga aaatatggta
     1561 agtgacggtg gaaatggaat tacaatacat aataaattag tagaaattaa acccttagat
     1621 tacgataaat gtatcaattt aattaatgaa tgtaaggaaa aaggatatat ttgggcaatt
     1681 tcacctgata atgcgacaag acgattggca ccagataatc gcttttatga ttttacacat
     1741 gatatctata tggataccat agttcaagaa ggtttggatc caaaagactt tgataaaatt
     1801 tataaagtat atgtggcttg ttatgctcca gaggaagaaa agctggaaac tttaaaggaa
     1861 ctgccttggt gtcgttttca tcaagaatac ttgtttgtag aacctggcga taagtcagtt
     1921 ggaatcaaaa tgatggttga tcattttgat ggggattata gtgatgtag
//
LOCUS       DAOMTA010000630         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2886_length_1967_cov_1.770397, whole genome shotgun sequence.
ACCESSION   DAOMTA010000630 DAOMTA010000000
VERSION     DAOMTA010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_1967_cov_1.770397"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLK68_13805"
     CDS             complement(<1..262)
                     /locus_tag="PLK68_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91522.1"
                     /translation="MRMKKRGIMKKGLILGLSCMMLGSTITITRAAENTKPLTDFGNV
                     LDVTADPKEKIYGTYSTNEYNNFSDMGAWHGYYLHQKTATDLY"
     gene            complement(489..>1235)
                     /locus_tag="PLK68_13810"
     CDS             complement(489..>1235)
                     /locus_tag="PLK68_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRM91523.1"
                     /translation="ENADNAIAKACQATNSEMIDYTVAPIFMNGKEKGAHEWIIEFKN
                     KPDNIENFRKALDENLQALYSFARLQHNSLFLAGIVLETIPRYTLRKETFVDVAIIKI
                     PVSLEQYKLANEIIGELYGNEEYLYNLFSVLTYPVTKGFATYQSYTCVEFVIHVLSQI
                     GFKFSAPGYYYKPDDLLTIFKDNIYFEGNLLDYCPDERIDEQYFAPMSFEVAKKSAKC
                     FGKIVGRSIFCRGDDYFKNKEMFWDKNNFH"
     assembly_gap    1236..1245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1461..>1967)
                     /locus_tag="PLK68_13815"
     CDS             complement(1461..>1967)
                     /locus_tag="PLK68_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRM91524.1"
                     /translation="RMAYIDDISNGVIHTIYNNEYNLRQALKTADLVIGAVLIPGAKA
                     PKLVTEDMVKTMKEGSAIVDVAIDQGGCIETCDHITTHDDPVFIKHGVLHYSVANMPG
                     AVPRTSTIALSNATLPYILKLADKGIEALKEDAGFMKGLNTYKGYFTCKPVAQALNGK
                     YKEVEELL"
BASE COUNT          635 a          363 c          259 g          700 t
ORIGIN      
        1 catatagatc tgttgctgtt ttttgatgta gatagtaacc atgccaagct cccatatctg
       61 aaaagttatt atattcattt gtactatatg tcccgtagat tttttctttt ggatcggcag
      121 ttacatctaa aacatttcca aaatcagtaa ggggttttgt attctcagct gctcttgtaa
      181 tcgttattgt agagcccaac atcatgcatg acaatcctaa aatcagtcct tttttcatga
      241 tacctctttt tttcattctc atcgattttc tcctccatat aaaactgtaa aaagatgtat
      301 gctataggat aaagatgaac gcgcgctttt tcatcaaaaa aggcatcaac atatactaaa
      361 aaaatatata aagataaaaa atatcttaat ataaatctag tatagttgat gcctgcatta
      421 ccagttacac actatttcaa tttttacaaa ctaaatacta tcattgtaaa cggttactgt
      481 caaccccttt agtgaaaatt gtttttatcc caaaacattt ccttgttttt aaaataatca
      541 tcacctcgac aaaatatact gcgtccaact attttgccaa aacatttggc actcttttta
      601 gcaacttcaa aactcattgg tgcaaaatac tgttcatcga ttctttcatc tgggcaataa
      661 tccaagagat tcccctcaaa ataaatattg tctttaaaaa tagtaagtag atcatcgggt
      721 ttataataat atcccggggc actgaattta aaaccgattt ggcttaacac atggataaca
      781 aattcaacac atgtatatga ttgatatgta gcaaatccct ttgttaccgg gtaagtaaga
      841 accgaaaaaa ggttatataa atattcttca ttaccataca actccccaat aatctcatta
      901 gctaatttat attgttctaa actaaccgga atttttataa ttgcaacatc aacaaatgtt
      961 tcttttctta atgtatagcg aggaattgtt tcaaggacaa tcccggctaa aaataatgaa
     1021 ttatgctgta gacgagcaaa tgaatataac gcttgaagat tttcatctaa tgcttttctg
     1081 aaattttcaa tattatcagg tttgttttta aattcgataa tccattcgtg agctcctttc
     1141 tctttcccat tcataaaaat aggagcaacg gtataatcaa tcatttcact attggtggcc
     1201 tgacacgctt tggcaatagc attatctgca ttttcnnnnn nnnnnactat gattatagcg
     1261 aatccttcca acttttctaa tcatgtaacc aaatcttgtt ggtgttttgg aaaccattaa
     1321 atataaattt tttgttttat ccatcgttag attcccttca atatttttta tcttaatatt
     1381 ataaccatac catatatttt aaatatcgac aaattttgtt caattttaga aaaaatacaa
     1441 gtcccgttaa ggacttgtat ttaaagtaat tcttctactt ctttatattt accattaagt
     1501 gcttgagcta ccggcttaca agtaaaatat cctttataag tatttaatcc tttcataaat
     1561 ccagcgtctt ctttaagcgc ctctatacct ttgtcggcaa gttttaaaat atatggtaaa
     1621 gttgcatttg atagagcaat agttgaagtt cttggtactg ctccaggcat attagccact
     1681 gaataatgta atactccatg ttttataaaa acaggatcat catgagtagt gatatgatca
     1741 caagtttcaa tacacccacc ttggtcaatt gcaacatcga caatcgcact accttctttc
     1801 attgttttaa ccatatcttc ggttactaat ttaggggctt tagctccggg aattaatacc
     1861 gctccaatta ctagatcagc tgtttttaga gcttgtcgca aattatattc attattataa
     1921 atcgtatgaa taaccccatt actaatatca tcgatatatg ccatacg
//
LOCUS       DAOMTA010000631         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2888_length_1966_cov_2.196232, whole genome shotgun sequence.
ACCESSION   DAOMTA010000631 DAOMTA010000000
VERSION     DAOMTA010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2888_length_1966_cov_2.196232"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..794
                     /locus_tag="PLK68_13820"
     CDS             309..794
                     /locus_tag="PLK68_13820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRM91525.1"
                     /translation="MKRALVVIDIQNDITKNYKGIIDNINRAIDWAVDKGVYVVYIRH
                     ENLSDGTRTFKPNTHGAELVPDLKIVSKNVFTKYKGNALSSEEFADFINKNGVDEFYI
                     TGADAIACVKSTCYNLTKANYTVNVLSDCITSYDKKKIDEMIRYYESKGCKIISLNEL
                     S"
     gene            791..1360
                     /locus_tag="PLK68_13825"
     CDS             791..1360
                     /locus_tag="PLK68_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRM91526.1"
                     /translation="MTTETEIKYFEDREDWRKWLTDNFETKGGIWFVFPNKSSGKKGI
                     TYNDAVEEALCFEWIDSTVRSLDQEHKIQHFTPRNPKSGYSQANKERLKWLLENKMIY
                     SKFEDKIRKILSVPFVFPNDIIDRLKENEIVWENYQHFSDGYKRIRIAYIEAARKRPE
                     EFEKRLNNFIHKVEDNKMIKGFGGIEKYY"
     gene            complement(1713..>1966)
                     /locus_tag="PLK68_13830"
     CDS             complement(1713..>1966)
                     /locus_tag="PLK68_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91527.1"
                     /translation="WSSNSRWFVFASKRDDGLYGKPYFCYIDRNGKAHKPFVLPQSDP
                     SFYDLNLKSFNIPELSRGKIPFNAVDVKRLMDKKSEKFK"
BASE COUNT          721 a          269 c          369 g          607 t
ORIGIN      
        1 ggtgaaattt tcacatcccc ggtgtttttc catatatgtt agcatctcat aatccatttt
       61 tatcttctta ctttcatcat tatggactaa gaaaatttta ttggaaaaat aatgagttcg
      121 tacaaggttt taatacaaca attttgtatc tttgtactta ttgaagggag cttataccta
      181 aagttgagaa tagactaaaa agcataacta gtaaagatgg gaaataatat acaagaaact
      241 ttcttgcatt cactaaccat atcgctagaa acataaatcg tcagacacgg acataaaaag
      301 taagaaaaat gaaaagggca ttagtagtaa ttgacattca aaatgatata acaaagaatt
      361 ataagggaat aattgacaat ataaatagag ccatagattg ggctgttgat aaaggtgttt
      421 atgttgttta tataagacat gaaaatttat cagacggtac aaggactttt aaacctaata
      481 cacatggggc tgaattagtt ccggatttga aaatagtatc aaaaaatgtt ttcacaaaat
      541 acaaaggaaa cgcattaagt agtgaggagt tcgcagactt tattaataaa aatggagtag
      601 atgaattcta cataacagga gctgatgcga ttgcttgtgt caagtcaact tgttataact
      661 tgactaaggc aaattataca gtaaatgtct tatcagattg cattacgagt tatgataaaa
      721 agaaaataga cgaaatgatt cgttattatg aaagtaaagg ctgtaaaata attagtttga
      781 atgaactatc atgacaacag agacagaaat aaaatatttt gaagatcgag aagattggag
      841 aaagtggctg acagataact ttgagaccaa aggtggaatt tggttcgtat ttcctaataa
      901 atcatcaggc aaaaaaggta ttacatacaa cgatgctgtt gaagaagccc tttgtttcga
      961 atggattgac agtacagtta gatcgcttga tcaagaacat aaaattcagc atttcacgcc
     1021 tagaaatcct aaaagcggat actcgcaagc caataaagaa agacttaaat ggttattgga
     1081 aaataaaatg atatactcta aatttgagga taaaatacga aagatcttgt ctgttccttt
     1141 tgtttttcct aatgatataa ttgatagatt gaaagaaaat gaaatagtct gggaaaacta
     1201 tcaacacttt tcagatggat ataaacgtat tcgaattgca tatattgaag cagcaaggaa
     1261 acgaccggaa gaatttgaaa agaggttaaa caactttatc cataaagtag aagataataa
     1321 aatgataaaa ggatttggtg gaattgaaaa atactattaa tcacaataaa attcaggcac
     1381 ctaataattg ttattattgc aagtgtgttg caatagttaa tcactaacaa tgttatagct
     1441 ataagaatac ttactaatct aatttgaatt ggtcatcaat ctaacttgga ttggtaacca
     1501 atctaacttg aattgatctt taagtcaagt tgatttagca tgtaatatgt atgcttttat
     1561 ggtgtgatat attatcaggt gcaatgaaat aaaaccgggg aggtgaaaat ttcacttccc
     1621 cggagataaa tatttgatat gaaggctgtt acgctgtttt gtaccagagg gaggtgaaat
     1681 cgctttcttt tttgataaat agtagatact ggctatttga atttctctga ttttttatcc
     1741 attaacctct ttacatccac agcattaaaa ggtattttac ctctgctgag ttctgggata
     1801 ttgaacgatt ttaggttgag gtcgtagaaa ctagggtcgc tctgtggtag aacgaatggc
     1861 ttatgcgctt ttccgttgcg gtctatgtag cagaagtacg gtttgccata tagaccatca
     1921 tcacgcttgc ttgcaaatac aaaccaacgg ctgttagacg accaac
//
LOCUS       DAOMTA010000632         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2899_length_1963_cov_1.996855, whole genome shotgun sequence.
ACCESSION   DAOMTA010000632 DAOMTA010000000
VERSION     DAOMTA010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_1963_cov_1.996855"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..555
                     /locus_tag="PLK68_13835"
     CDS             88..555
                     /locus_tag="PLK68_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91528.1"
                     /translation="MSVSNETGFFHSLTLLNHWVCTYLPHFTLTKSISDNVPSVTIVL
                     VGIVENINELLDEHQSLDRCLKQVKMPKMKKDECLEIITSGMKKLEINISPTISQKIV
                     DVSSGYPYPVHLICYSGVLKIIKNNKNEFLQEYFRLAINKAIKTSSESLKMHM"
     gene            1049..1477
                     /locus_tag="PLK68_13840"
     CDS             1049..1477
                     /locus_tag="PLK68_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM91529.1"
                     /translation="MEKYDIDFLGYRLDTDVSTLPTFSGIYIVYRCIYSSIMKIVDIK
                     ELLYIGQATNIHDRLSNHEKKPSWEKRCSQDEVLCYAYANIAINDLDVVENGLIFMQK
                     PIMNTDLCNDYIHPIPVAFEVTGKCANFFQTSFSIVGKTK"
     gene            1488..>1963
                     /locus_tag="PLK68_13845"
     CDS             1488..>1963
                     /locus_tag="PLK68_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91530.1"
                     /translation="MTLIITKSKRKKVTHWLDSHLGISLFLREIKVSFFSTLVPNYVV
                     LSYSIPSLKSWNLQRLSELSINFPPIVYAIIIIILTFIIPHLIQMHKQNQEKSLSKVL
                     SNLLGGITVVVNDKSRRFHTVIHQNEHGDDKMSKDEIFTTITQPKEQIHSICTAIMT"
BASE COUNT          739 a          308 c          300 g          616 t
ORIGIN      
        1 acaattaact aatttggatt acaatgtggt tgcattatcc gcttgaattc agctcttatg
       61 aagaaaatga gaacttttgt agattgaatg tctgtttcta atgagacagg cttttttcat
      121 tcgctcactc ttctaaatca ttgggtatgt acctaccttc ctcattttac cctcacaaaa
      181 tccatatccg ataatgtacc ttctgtaact atagtgttag ttggtatagt tgagaatatc
      241 aatgaattat tggacgaaca tcaatcatta gatagatgcc taaagcaagt taaaatgcct
      301 aagatgaaaa aagatgaatg tctcgaaatt atcacttctg gaatgaaaaa acttgaaatt
      361 aatatttctc cgacaatttc tcaaaaaata gttgatgtat cttctggata cccatatcct
      421 gtgcatctaa tatgctattc aggtgtactt aaaataatta aaaataacaa aaatgaattt
      481 ttacaagaat atttcagact tgcgataaac aaagcaataa aaacatcatc tgaatcttta
      541 aaaatgcata tgtaaaagct gtaggaatga acagaaataa taatatatgg ccaaacatct
      601 taaatatctg cgtaatgttt gaatctgatg agaatgagga gttttcaaaa gagaaaatat
      661 ttgcacatat tgctttcaaa accttttcag cttgtgaggt aactgtgggg gaaaaacttt
      721 ccacacgtat atgagcccgt taaaataaaa aaaccgaaat aaattcattc gaaaaaggat
      781 tgattttgtt ttttaaaata tatttcccaa attttgacaa aatattaaaa cactttaaag
      841 aaacttcttt ggtagtataa aaaaaatata tacttttgtc cgttgaatcg tgctaaaact
      901 tggtaagcac ctttctgatt ccgaaaaaga atgaatgact atacgcataa taaaaagaca
      961 atccccataa ttgatcttta gaattgaaaa aattatatag aattttataa tcaatagcat
     1021 taatttaaat ggataattcc tataataaat ggaaaaatat gatatagatt ttctgggata
     1081 tcgcctagac actgatgtat ctacattacc aacattttca ggcatataca ttgtttatag
     1141 atgtatatat agttccatta tgaaaattgt ggatattaaa gaattgcttt atataggaca
     1201 agcgacaaac attcatgata gattgtcaaa tcacgagaag aagccatcat gggaaaaaag
     1261 atgttcgcaa gatgaagttt tatgttatgc ctatgcgaat atagctataa atgaccttga
     1321 cgtagtcgaa aacggtctaa tattcatgca aaaaccaatt atgaatactg atttgtgtaa
     1381 tgactacata caccctattc cagttgcatt tgaagtaact ggcaaatgtg caaatttttt
     1441 tcaaacaagt tttagcatag tcggaaaaac aaaatagaag taaagccatg acattaataa
     1501 ttaccaaatc aaaaagaaaa aaggtaacac attggttgga ctctcacttg gggatcagtc
     1561 tgtttctccg cgaaatcaag gtctcatttt tcagtacgtt agtacccaac tatgtagttc
     1621 tctcctactc aataccgtca ttaaagtcat ggaatctaca aagattatca gaacttagta
     1681 taaatttccc tccaattgtt tatgccataa tcattattat tctgacgttt attattccgc
     1741 atttaataca gatgcacaaa caaaatcaag aaaaatctct aagtaaggta cttagcaact
     1801 tattgggagg aattacagtg gtggtaaatg acaaaagtcg tcgcttccat actgttatac
     1861 atcaaaatga acatggcgat gacaaaatgt ctaaagacga gattttcaca actataactc
     1921 agccaaaaga gcaaatacat tctatctgta cggcaataat gac
//
LOCUS       DAOMTA010000633         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2914_length_1957_cov_1.981598, whole genome shotgun sequence.
ACCESSION   DAOMTA010000633 DAOMTA010000000
VERSION     DAOMTA010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2914_length_1957_cov_1.981598"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..>624
                     /locus_tag="PLK68_13850"
     CDS             8..>624
                     /locus_tag="PLK68_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM91531.1"
                     /translation="MKIKKSIFILMVIVLITILGTVLYKGEKYNLIIIASFALSCLPF
                     YFKYENSKPKIREVVVLATMIALTVVSRTIFMLTPSFKPVTAMVIICGIVFGPASGFM
                     CGSLSATLSDFVFGIGPWTPFQMIAWGIIGYGAGIFSKNLFKNKFLLYGYSAICGLAF
                     SMVMDLWSVLAFETSFNLTRYLAIVLTSLPTTLIYIVSNIIFMFLL"
     assembly_gap    625..636
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            690..1811
                     /locus_tag="PLK68_13855"
     CDS             690..1811
                     /locus_tag="PLK68_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRM91532.1"
                     /translation="MKKLMRFTVALALVCACIVPINAQSSTLAKAQSYWLNNNELSGL
                     DAVLAVESLGLDVEDEKNNFTLNYSFEAPTNSYGDEITYEEMDAGYLAKNIMALAAIK
                     SDPAKLKLKDGSTINLIDLLKSKIDENGNVDYGSANPESSTAYTMFALAIVDESYNLD
                     KLGLNLTEMQLSDGSWGYNGAWGGPDITGWALAALSLCNNTYQASIDKALAYLASIQI
                     DNGGYSGFGVNCNSQACAVWGILEYDIQGVKNGTYNKGTGNPYDLLLQFMQEDGSFGA
                     SLDVDYGNAYATVQAALTVGVYENGSLIDNIKAQYDEMLNPKNDQPEPTPAPAPVTPS
                     TPADKSITSVKTGDDAVIALTVSSLIISGGMYLFIRKES"
BASE COUNT          628 a          259 c          374 g          684 t
ORIGIN      
        1 tagggttatg aaaataaaaa aaagtatctt tattttaatg gttattgttt taataacgat
       61 cttaggaact gttttatata aaggtgaaaa atataattta attattattg cttcgtttgc
      121 attatcttgt ttaccatttt attttaaata tgaaaatagc aaacctaaaa taagggaagt
      181 tgtggttttg gcaacaatga ttgccctaac agttgtttct agaacgattt ttatgttaac
      241 acccagtttt aaacctgtaa ctgcaatggt tattatttgt gggatagttt ttggtccggc
      301 atctggtttt atgtgtgggt cgttgtcagc aacattatca gactttgttt ttggaatagg
      361 accatggact ccattccaaa tgatcgcatg gggaataatt ggttatggag ctggaatttt
      421 tagtaaaaat ttgtttaaaa ataaattttt attatatgga tatagtgcta tttgtggact
      481 tgctttttcc atggtaatgg atttatggtc agttcttgct tttgaaacaa gttttaattt
      541 aacacgatat ttggcaatag tattaacttc attaccaacg acgttaatat atattgtttc
      601 aaatataatt tttatgtttt tactnnnnnn nnnnnncaga tcttacaaag ggtaaaaata
      661 aaatatggaa taacggaggg aaaaattgaa tgaaaaaatt aatgcggttt actgtggcgt
      721 tagcgcttgt ttgtgcttgc attgtgccaa tcaatgcgca atcttcaact ttagccaaag
      781 ctcaaagcta ttggttgaat aacaatgaat tatctggact ggatgccgtt ttggctgtag
      841 aatctttagg attagatgtt gaagatgaaa agaataattt tactttaaac tattcttttg
      901 aagcaccaac aaattcttat ggtgatgaaa ttacttatga agaaatggat gctggttatt
      961 tggctaaaaa tattatggct ttagcggcaa taaaatcaga tcctgctaag ttaaaactaa
     1021 aagatggttc aacgattaat ctaattgatt tattaaaatc aaaaattgat gaaaatggta
     1081 atgttgatta tgggtcagca aatccagaat caagtactgc atatacaatg tttgccttag
     1141 ctattgttga tgaaagttat aatcttgata aactaggatt aaacttgact gagatgcagc
     1201 ttagtgatgg ctcttggggc tataatggag cttggggtgg acctgatatt actggatggg
     1261 cattagctgc tctttcatta tgcaacaata catatcaggc ttcaattgat aaagctttag
     1321 cttatttagc atcaattcaa attgataatg gcggttatag tggctttggc gttaattgta
     1381 atagtcaagc ttgtgccgta tggggaattt tagaatacga tattcaaggt gtaaaaaatg
     1441 gtacttacaa taagggtaca ggcaatccat atgatctatt attacaattt atgcaagaag
     1501 atggttcatt tggcgctagt ttagatgtag attatggcaa tgcttatgca acagttcaag
     1561 cggcccttac tgttggggta tatgaaaatg gttccttaat tgacaatatt aaagctcaat
     1621 atgatgagat gttgaatcct aaaaatgatc agccagaacc aacaccagct cctgctccgg
     1681 ttacaccatc tacacctgct gataagtcaa ttacatcagt taagacaggt gatgatgcag
     1741 tcattgcact aacagtatct tcattgatta ttagtggtgg aatgtatttg tttattagaa
     1801 aagaatctta aaaaagtgta aaatgtagtg acccccaaaa tatagacttt tcaaatctat
     1861 atttcggagg tcttttctta ttgtcaaata ttccatagaa ttaaaacaac gcgttattca
     1921 agattattta tttggcaagg gaggctctac ctatctt
//
LOCUS       DAOMTA010000634         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2919_length_1956_cov_2.173593, whole genome shotgun sequence.
ACCESSION   DAOMTA010000634 DAOMTA010000000
VERSION     DAOMTA010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_1956_cov_2.173593"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLK68_13860"
     CDS             complement(<1..446)
                     /locus_tag="PLK68_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91533.1"
                     /translation="MYNKEGTVVDGTQVRTLYTVFSSDGKYHTNTKPILEPGVYTETV
                     YTLGSNYAVKPITRVYTIRKETVQIKFDNYLNVETYDGTSKSLIGKVYDKSGNAFIDA
                     KLVYKGVDNSYNSTIPPTAAGRYRVVASYSGDDTHYAAIPKVASLV"
     gene            complement(496..786)
                     /locus_tag="PLK68_13865"
     CDS             complement(496..786)
                     /locus_tag="PLK68_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91534.1"
                     /translation="MVIFYLFKLYRSLWQFASINELKNILSAAVIDSIANVFLFEMAD
                     KSLPLSCYFIYFLLITMFLGITRLIYRMTRLYRTEKGSDNYKERRSLEKVRD"
     gene            complement(957..1682)
                     /locus_tag="PLK68_13870"
     CDS             complement(957..1682)
                     /locus_tag="PLK68_13870"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRM91535.1"
                     /translation="MLGRKRKKAVKSTHKIIDKSNKNNHVDYQEVYRNIRTNIEYSAV
                     GKNVKAINITSSISNEGKSTTALNLAMIYATKYANVLLIDADLRRPTQHHYLKLSNSR
                     GLTNALIKYGETKKISSKYFQFIEDESFEGKLSVLTAGIKVPNPSELISSDIFEEFIN
                     ELMKLYDFIVIDCPPVMLVSDAIPIGNVVDGTVFVCSSQLTGRKDAKASIEILQKNNV
                     NILGTVLSQVEVEKDKYNNYYYY"
BASE COUNT          671 a          354 c          225 g          706 t
ORIGIN      
        1 ataactaaag aagctacttt cggtattgcc gcataatgtg tatcatcacc actatatgac
       61 gctacaacac gatatcgacc agctgcagtt ggggggatag tactattata actattatca
      121 acacctttat atactaattt agcatcaata aaagcgttac ccgatttatc ataaactttt
      181 ccaattaatg attttgatgt accatcatat gtttccacat ttaaataatt atcaaactta
      241 atttgtactg tttcttttct aattgtatat acacgagtaa ttggttttac tgcataatta
      301 ctccctaaag tatatactgt ttcagtatat acacctggtt ctaaaattgg ttttgtatta
      361 gtgtgatact taccatcact agaaaaaact gtatatagtg ttctaacttg agtcccatca
      421 acaacagtac cttctttatt gtacaattta ccaccaaaat tgaaattgta tctatcactg
      481 tatactatgc tgcctttaat ctcttacttt ttccaaggag cgtcgctctt tataattatc
      541 actgcccttt tctgttcgat ataatctagt cattcgataa atcaatcttg ttatccctaa
      601 aaacatggta atcaataaaa aatatataaa atagcaactt agcggtaaac ttttatcggc
      661 catttcaaat aaaaaaacat tagcaatact atcaattact gcagcgctaa gaatattttt
      721 aagctcatta attgaagcaa attgccataa actacggtaa agtttaaaaa gataaaaaat
      781 caccaacgag ataaaaatct ccagtcccat taaattcttt aatatattaa aataatagct
      841 tggaatctct ccattaaacc taagaaatag cgctaaaaac ttgctagaat aatgcaaatt
      901 aaatcaatta aaattaaaat aattcttctt acactaaact ttctcattgc ccctccttaa
      961 taatagtaat aattattgta tttatccttt tcaacttcta cttgacttaa tactgttcca
     1021 aggatattga cattattctt ttgtaagatt tcgattgaag ccttagcatc tttacgacca
     1081 gttagttgtg atgagcaaac aaaaaccgtt ccatcaacca cattaccaat cggaatagca
     1141 tcactaacta acataactgg tggacaatca ataactataa aatcatacag cttcatcagc
     1201 tcattaataa actcttcaaa gatatcacta ctaattaact cactaggatt tggtaccttg
     1261 atgccagcag ttagtactga taactttccc tcaaatgatt catcttcaat aaattgaaaa
     1321 tattttgaac taattttttt agtttcacca tatttgatca aagcgttagt taatcctcgt
     1381 gagttcgata gttttaaata atgatgttgg gtcggtcttc ttaaatcagc atcaatcaat
     1441 aagacatttg catatttcgt tgcatatatc atcgctaagt ttaaagccgt agtgctcttt
     1501 ccttcattac taattgaaga tgtgatatta attgctttaa cattttttcc taccgctgaa
     1561 tattcaatat tagtcctaat gttacgatat acttcttgat aatcaacatg attatttttg
     1621 ttacttttat caataatttt atgtgtactt tttacggcct tttttctctt tcttcctaac
     1681 attttctctc tcctttgaaa actattcacc agtcatcata cagctttaat aaattggcag
     1741 ttctaccagc tgttaaacat atgaccaggg aagtaaatta gactatcaca aaataatttt
     1801 gatttatttt acagagacaa gatgtgttgt ttctatattt tctagtacta ttcatgaaaa
     1861 tctaattata tatatttatc actagaacaa ataaactatc atacttggta ttttaaacat
     1921 tcaactaatc tgtgataata acataagcca aaaaat
//
LOCUS       DAOMTA010000635         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2920_length_1956_cov_1.945818, whole genome shotgun sequence.
ACCESSION   DAOMTA010000635 DAOMTA010000000
VERSION     DAOMTA010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2920_length_1956_cov_1.945818"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLK68_13875"
     CDS             complement(<1..1003)
                     /locus_tag="PLK68_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91536.1"
                     /translation="MKKKELRKDFLWGAALSNVQAEGGYLEDGKGLNVYDTLVVTPEP
                     GIESQYSDTKVATDHYHHFKEDIDLMAEMGIKAYRFSIIWSRIHPNGDDEANEKGLDF
                     YEEMIDYMLSKGIEPVVSLVHFDMPDHLMRAYNGFLNKKVIDFYVEHVRCIAKRMNDK
                     VKYWITYNETNLAPYQSDLVAGTNRPDYMSKEEFYSQLYINIQVAHARAVLAIKKEIP
                     DAMVGGMVNYCLFQPATTTSRDMIACEFSHKFMNYLSFDIMTSGYLPEYFVSFMEKRD
                     IECEFNNEEQEDVLKASKELGYLAISYYRSMMVTSYRTIKNESIIDFENNLLWGKGFV
                     "
     gene            complement(1015..1941)
                     /locus_tag="PLK68_13880"
     CDS             complement(1015..1941)
                     /locus_tag="PLK68_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRM91537.1"
                     /translation="MSILFFKPIPRPAIWGLTLVKDYFDYCDFPDGIGQSWSFSAQEN
                     ASTVCITEPFVGKTLHELWNSHQELFGHPNEPFPVIISLVGPEDDLSIQVHPDNIYAL
                     KEGFNSGKNEAWYFIEAMPNYNIVYGHNASSPEDLKDYIRNEHWDDLIRYLDVKSDDF
                     VYLPAGLLHALRKGSVVYEIQQATDITYRFYDYHRKDSQGNERELHLDKAIECLNYDQ
                     SKMENNVQPIEKKYDNIKETVYISNDSFTVTKLEVSGLSSYIYNNYQLATVVKGCGKA
                     DGVEIKMGDNFIIPINNKVEFDGQMIIMMTTK"
BASE COUNT          638 a          378 c          242 g          698 t
ORIGIN      
        1 gtacaaatcc tttcccccat aaaagattgt tttcaaaatc tattattgat tcatttttaa
       61 tagttctata tgatgtaacc atcattgaac gataataaga tatagctaaa tatcctaatt
      121 ctttagaagc cttaagtaca tcctcttgtt cttcattatt aaattcacat tcaatatctc
      181 ttttttccat gaaagaaaca aaatattccg gtaagtatcc tgatgtcata atatcaaagg
      241 aaagataatt catgaattta tgcgagaatt cacaagcaat catatccctt gaagtagtag
      301 ttgcaggttg gaacaaacaa taattaacca ttccaccaac catagcatca ggtatttctt
      361 tttttattgc taaaactgcc cttgcatgag caacttgaat attaatatat agttgagaat
      421 aaaattcttc tttactcata taatcaggcc tattagttcc agcaaccaaa tcactttgat
      481 atggagcaag atttgtttca ttataagtaa tccagtattt aactttatca ttcattcttt
      541 tagcaataca ccttacatgt tcaacataaa agtctataac ttttttattt aaaaaaccat
      601 tatatgccct catcagatga tcaggcatat caaaatgaac taaggaaaca actggttcaa
      661 ttcccttcga taacatataa tcaatcattt cttcataaaa gtctaatccc ttttcattag
      721 cttcatcgtc gccattagga tgtatccgtg accaaataat tgagaaacgg taagctttaa
      781 tgcccatttc ggccattaaa tcaatatcct ctttaaaatg atgataatga tctgttgcaa
      841 ccttagtatc actatattga gattcaatcc caggttcagg tgtaacaaca agtgtgtcat
      901 atacatttaa tccctttcca tcttctaggt agccaccttc tgcttgaaca ttgcttaacg
      961 cagctcccca taaaaaatct tttcttaatt ctttcttttt catcgtcttt cctcttattt
     1021 tgtggtcatc ataattatca tctgaccatc aaattctact ttattattta taggaatgat
     1081 aaaattatct cccattttta tttcaacacc atcagccttt ccacaacctt taacaaccgt
     1141 tgctagttga tagttattat aaatatagct actcaatcca cttacttcta actttgtaac
     1201 tgtaaatgag tcattggata tatagacagt ttccttaatg ttgtcatatt ttttttcaat
     1261 tggttgaaca ttattttcca tttttgattg atcataattt agacattcaa tagctttatc
     1321 taaatgtaat tcacgttcat taccttgaga atcttttcga tgatagtcat aaaaacgata
     1381 agtaatatct gttgcttgtt gtatttcata aactacactc ccttttctca aggcgtgtaa
     1441 caatccagcc ggcaaatata caaaatcatc agatttcaca tctagatatc ttattagatc
     1501 atcccagtgt tcgtttctta tataatcctt taaatcttct ggacttgaag cattatgacc
     1561 ataaacaata ttgtaatttg gcattgcttc aataaagtac caagcctcat ttttcccgct
     1621 attaaaacct tcttttaaag cataaatatt atcgggatgt acttgaatac ttaaatcatc
     1681 ttcaggacca accaatgaaa taataactgg gaatggttcg tttggatgac caaacaattc
     1741 ttgatgactg ttccataatt catgcaatgt cttaccaaca aatggttcag taatacaaac
     1801 tgtagaagcg ttttcttgcg cgctaaaaga ccatgattgc cctataccat ccgggaaatc
     1861 gcaatagtca aaataatctt tgacaagtgt aagtccccat attgcaggac gaggtatagg
     1921 cttaaaaaat aatatactca tttatattct cctttg
//
LOCUS       DAOMTA010000636         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2927_length_1952_cov_2.507644, whole genome shotgun sequence.
ACCESSION   DAOMTA010000636 DAOMTA010000000
VERSION     DAOMTA010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_1952_cov_2.507644"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PLK68_13885"
     CDS             complement(<1..1763)
                     /locus_tag="PLK68_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM91538.1"
                     /translation="MKTLKTILVLLIMSMLINIIPIKAASRTVKVAYPIQSGLTEIDE
                     DGLYSGYTYEYLKEISRFTNWNLEFVRLEGTVNEQLSSAMEKVKTGEIDLMGGMLYID
                     TLINDYDYSATNYGMGNMAIYVNSNNAEINDTSIYSLKNLNVGVVSTKKETNVNLKDF
                     GDINGININQIFFDNTPDLLTSLERNEIDAMVLSDLSIEEGNYRVVARFSPRPFYFVM
                     TKGNQELMSELNEAMTQLNKEQPNYMSNLHERYFSLANRNFILTEAEKGFVANNSKID
                     VLILGGKAPIQYYDEKKGKVVGITVDVLNYIGKLSGLKFNYIYANDIDEYNKQIETKK
                     PMIIGGVYNSNPEKYITTKAYLDSAMTLVTNKNINANELDGKQAAMIYDSEVDSAAVG
                     DSSNKVNYYGTQLECIKAVENGTADYTYINNHVALFYNSNYDFKNINIIPQGNLRNQG
                     SYFAINTGVADELLDIINKGIDVVSYSKIEEIIFTNASTSENDVTFFDYVENNQLQVA
                     TFTLMAVAIIVLFFLIWRNYINKRNNEKILSEYKRYQQISEFSHDCFVEYNIATDTLV
                     LMGGGAKLLSKETIIKDFLKRP"
BASE COUNT          645 a          368 c          238 g          701 t
ORIGIN      
        1 ggtctcttta aaaaatcttt aataatcgtt tcctttgaaa gcagtttggc tccacctccc
       61 attaaaacaa gggtatcagt tgcaatgtta tattcaacaa agcaatcatg agaaaattca
      121 ctaatttgtt gataacgctt atactcactt aaaatctttt cattattacg tttatttata
      181 taattacgcc aaatcaagaa aaacagcaca ataatggcaa ctgccataag cgtaaaagta
      241 gctacttgaa gttgattatt ttcaacatag tcaaagaaag taacatcatt ttcactggta
      301 ctggcattag taaatataat ttcttcaatc ttactgtaag atacgacatc aattccttta
      361 ttgataatat ctaaaagctc atccgcaaca cctgtattaa ttgcaaaata actgccttga
      421 tttcttaaat tcccttgagg aataatatta atattcttga aatcataatt actattataa
      481 aacaaggcaa catgattatt tatatacgta tagtcagctg tgccgttttc taccgctttg
      541 atacattcta actgagttcc ataataatta actttgttac tactatctcc aactgcggca
      601 ctatcaactt cagaatcata aatcattgct gcctgtttac cgtctagctc attagcatta
      661 atatttttat tagttactag cgtcatcgct gaatctaaat aagctttcgt cgtaatatat
      721 ttttctggat tgctattata caccccacca ataatcattg gtttcttcgt ctcaatctgc
      781 ttattatatt catcaatatc attggcataa atataattaa acttcaaacc gctcagtttt
      841 ccaatataat taagcacatc aacggtaatt cctacaacct tgcctttttt ttcatcataa
      901 tactggattg gggctttccc acccaatata agaacatcaa tttttgaatt atttgcaaca
      961 aaacctttct cagcttcggt aagaatgaaa ttacggttcg ctaaagaaaa gtaacgttca
     1021 tgaagattgc tcatataatt aggctgttct ttatttaact gtgtcatcgc ttcatttagc
     1081 tctgacatca gttcttgatt tccttttgtc attacaaaat aaaatggacg aggcgaaaaa
     1141 cgtgctacga cccgataatt accttcttca attgataaat ctgataatac catagcatct
     1201 atttcatttc tctctaatga tgttaataaa tctggggtat tatcaaaaaa tatttgatta
     1261 atattaattc cattaatatc tccaaaatct tttaaattaa cattagtttc ctttttagtt
     1321 gaaacaactc caacatttaa attcttaaga gaatatatac tagtatcatt gatttcagca
     1381 ttattagaat taacatagat agccatattt cccatgccat aattggttgc cgaataatca
     1441 taatcattga ttaaggtatc aatatagagc atcccaccca ttaaatcaat ctcgcctgtt
     1501 ttgacctttt ccatagcgct tgacagctgc tcatttactg tgccttctaa gcgtacaaat
     1561 tctaagttcc aattcgtaaa tcgagagatt tcttttaaat attcataagt atagccggaa
     1621 tataatccat cttcatctat ctctgttaat ccagattgaa ttggataagc aactttaaca
     1681 gttcgggagg ctgctttaat tgggatgata ttgatcagca ttgacataat taacaatact
     1741 aaaattgttt ttagtgtttt catcaaacca ccccttttta catcacaata ttatcatatt
     1801 aattatacat taaattgatg aacattaaaa taaaaaggca caaatcatca gaataattca
     1861 tgcctgtatc aatattttat ctaattgttt taaaaactga tttcttactt catcagttct
     1921 tttagctggt ccacgtgtcc gcctaccact tt
//
LOCUS       DAOMTA010000637         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2961_length_1936_cov_2.065922, whole genome shotgun sequence.
ACCESSION   DAOMTA010000637 DAOMTA010000000
VERSION     DAOMTA010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_1936_cov_2.065922"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLK68_13890"
     CDS             complement(<1..242)
                     /locus_tag="PLK68_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein"
                     /protein_id="HRM91539.1"
                     /translation="MKSIKGWSDWYIHPFTLIYLIAAWLNDNLFAYLSALFIVCTHEF
                     GHYYFAKKFNFEIEKVEIFPFGAFLSLNDYGTHHII"
     gene            complement(217..795)
                     /locus_tag="PLK68_13895"
     CDS             complement(217..795)
                     /locus_tag="PLK68_13895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRM91540.1"
                     /translation="MSDLDDVRKRMQKRRGTNKKPLNDDNFKRFYNFMVKFMVVILVV
                     LVSMSYIKINPDSNIKKMILNDTNYKAVTSWISDNLFSFLPSDDVSVSSGVEYQHVKD
                     DYYKNNSNEVLSIEAGRVIATGNDENLSGYVVILGKNDVEITYSNIDNVMVTLYDEVD
                     QGMVLGSYQDQFVLQFEHLGKEISYEEYQRME"
     gene            complement(844..1623)
                     /gene="minD"
                     /locus_tag="PLK68_13900"
     CDS             complement(844..1623)
                     /gene="minD"
                     /locus_tag="PLK68_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRM91541.1"
                     /translation="MSRVIVVTSGKGGVGKSSVSVNLASALAFSKFKVCLIDGDFGLK
                     NLDVMMGLENRVVYDLNDVVEGRCTIEQVLVKDKRIDGLSLLPSCKSLSFENLDTEIM
                     NSLIERLNKDYDFIIVDSPAGVEKGFQYSASLANEAIVVVNLDVSSLRDADRVVGLLM
                     KKGINTINMIINKVNVDDIEGARSLTVEDAQEILSLPLLGIVYDSHDMIEANNRGVPI
                     FLNNQHLLHSCFANISKRILGQQVPYAKYKKKSLIRRFFYS"
     gene            complement(1616..>1936)
                     /locus_tag="PLK68_13905"
     CDS             complement(1616..>1936)
                     /locus_tag="PLK68_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRM91542.1"
                     /translation="KKNKIKVINKTVHAGEILELDQDALIIGQVNPGAIVRFKGKLYV
                     MGRVSGLVEGLNARAKISGQFFKNAHVRINGVSRRNYTSYELTMLYYKDNEIFLDKGD
                     MIYV"
BASE COUNT          685 a          407 c          202 g          642 t
ORIGIN      
        1 tcaataatat gatgagtccc ataatcattt aaactcaaaa aagctccaaa agggaaaatc
       61 tcaactttct caatttcaaa attaaatttt ttagcaaaat aatagtgtcc aaattcatga
      121 gtacagacaa taaacaaagc actcaaataa gcaaataaat tgtcatttaa ccaagcagcg
      181 attagataaa tcaaagtaaa aggatggata taccaatcac tccatccttt gatactcttc
      241 ataactaatt tccttaccta aatgttcaaa ctgtaaaaca aactgatctt gataactgcc
      301 taaaaccatc ccttgatcta cctcgtcata caatgtcacc attacattat caatattact
      361 ataagtaatt tctacatcat tcttgcctag aattactaca tatccgctca agttttcatc
      421 attaccagtt gcaattacgc gcccagcttc aatacttaat acctcattac tattattttt
      481 ataatagtca tctttaacat gctgatactc cacgccacta gaaacactaa catcatcact
      541 tggcaaaaaa gaaaataaat tatcagatat ccagcttgtt accgctttat aattagtatc
      601 attcaaaatc attttcttaa tattagaatc cggattgatt ttaatatatg acattgatac
      661 taaaaccact aaaattacga ccataaactt taccataaaa ttatagaatc gcttaaaatt
      721 atcatcattt aatggctttt tattagtgcc ccggcgcttt tgcattcttt ttcttacatc
      781 atctagatca ctcattctat cacctattcc attatatgaa tctgatgata ttaaagacgt
      841 taattatgaa taaaaaaatc gacgaattaa acttttcttt ttatatttag cataaggtac
      901 ttgctgcccg agtatgcgct ttgaaatatt agcaaaacag ctatgtaata aatgttgatt
      961 atttaagaaa atcggcacac cacgattatt agcttcaatc atatcatggc tatcgtaaac
     1021 aatccctagc agcggtaatg ataaaatctc ttgagcatct tccaccgtta atgagcgagc
     1081 tccttcaata tcatcaacat taactttatt aatgatcata ttaatagtat taattccctt
     1141 tttcatcaat aaaccaacaa cgcgatcggc atcacgtaat gatgatacat ctaaattgac
     1201 aacaacaatt gcttcattag ctaatgaagc agaatattga aaaccttttt cgactccagc
     1261 tggactgtcc acaataataa aatcataatc tttatttaat cgttcaatta atgaattcat
     1321 tatttctgta tctagatttt caaatgataa agatttacat gacggtaata agctcaatcc
     1381 atctatacgc ttatccttaa ctaatacttg ttcaattgta cagcggcctt caacgacgtc
     1441 atttaaatca taaactacac gattttctaa ccccatcatc acatccaaat ttttcaatcc
     1501 aaaatcacca tcaattaaac atactttaaa ttttgaaaaa gctaaggcac tagccagatt
     1561 aacgctaaca ctacttttac ctaccccacc ttttccggaa gtaactacaa taactctaga
     1621 catatatcat atccccctta tctaagaata tttcgttgtc cttataataa agcatcgtaa
     1681 gttcataact tgtataattt cgtcgagata ccccattaat tctaacatgg gcatttttaa
     1741 agaattgtcc actaattttt gctcgagcat ttaatccctc aaccaatcca ctgacgcgcc
     1801 ccattacata caactttcct ttaaatctaa caatagctcc gggattaact tgaccaataa
     1861 tcaaagcatc ttgatccaat tctaaaattt ctccagcatg aacagttttg ttgattactt
     1921 taatcttatt tttctt
//
LOCUS       DAOMTA010000638         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2968_length_1934_cov_1.968068, whole genome shotgun sequence.
ACCESSION   DAOMTA010000638 DAOMTA010000000
VERSION     DAOMTA010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_1934_cov_1.968068"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLK68_13910"
     CDS             <1..804
                     /locus_tag="PLK68_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM91543.1"
                     /translation="EGNTPDNLIGVHLEGPYLNIKNKGVMKEEHLRLVNFDEFNQYIS
                     TCRKVNAMTIAPELPNALELINYASNHGYVMNVGHSSASAKEVKKAQAYGAKGITHLY
                     NAMSQHLHRDPGVVTGAILSDLMCELIVDGFHIDEDVIRATYKAIGKERIILITDANP
                     CKGLPDGQYHFSGKDIVIVGGHATVKETGRIAGSTLGLNEACANMMRYCDCSIDDAVL
                     MAAVNPAKLYGLKQGKIEIGYQGDIVVIDKEFTILAVINRGIIKRNKFI"
     gene            935..1201
                     /gene="rpsO"
                     /locus_tag="PLK68_13915"
     CDS             935..1201
                     /gene="rpsO"
                     /locus_tag="PLK68_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRM91544.1"
                     /translation="MLTKAEKTAIMQKYATKEGDTGSPEVQIAVLTADINKLNGHFKQ
                     HPKDNHSNRGLLKKVGRRRDLLKYLKNKDIERYSSLCESLGLRK"
     gene            complement(1298..>1934)
                     /locus_tag="PLK68_13920"
     CDS             complement(1298..>1934)
                     /locus_tag="PLK68_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRM91545.1"
                     /translation="FKDCYYSKLKKYLFSLGLSIIIFITSIITANGLSYLSGNHHETS
                     SRFQINKVIESIELFFQGDFQLSLDQINQISSGRIELFNTSIEIWQTNPILGIGAGNF
                     QTIGRHLTNSNVVKQIQVVHSHNVFLETLVTTGIAGASLFIIFFIASFKSILKLFKVK
                     QQSSNYLKIMIFTLIVVSEFIGGMFDYGVFYVYSLSATLCWLFLGYLHDYH"
BASE COUNT          725 a          260 c          354 g          595 t
ORIGIN      
        1 gaaggaaata ccccagataa tttaattggt gttcatcttg aaggaccgta tttaaatatt
       61 aagaataaag gtgtaatgaa agaagaacat ttacgtttag taaattttga tgaatttaat
      121 cagtatattt caacttgcag aaaagtgaat gcaatgacaa ttgcccccga gctgcctaat
      181 gctttagaat taatcaatta tgcaagtaac catggctatg tgatgaatgt ggggcattca
      241 agtgcaagtg ctaaagaggt aaaaaaggca caagcatacg gtgcaaaagg aattactcat
      301 ctttataacg caatgtcaca gcatttgcac cgtgatcctg gagttgtaac tggtgcaatt
      361 ttatcagatt taatgtgtga gcttatcgtg gatggctttc atattgatga agatgttatt
      421 cgtgcgacat acaaagcaat tggaaaagag cgaattattt tgattactga tgctaatccc
      481 tgtaagggat tgccagatgg gcaatatcat ttttctggta aagatattgt aattgtcggg
      541 ggacatgcta cagtcaaaga aactggtaga attgcaggaa gcacattagg gttaaatgaa
      601 gcttgtgcta atatgatgcg ttattgtgac tgttcaatcg atgatgccgt actaatggcg
      661 gcagttaatc cagctaaatt atatggatta aagcagggga agatagaaat tggttatcag
      721 ggtgatattg ttgtcataga taaagagttt acaatcttag cagttatcaa tcggggaatt
      781 ataaaaagaa ataagtttat ttaaatattt agtgatttaa cttgacttta agaagatatt
      841 tgatagaata ttattcgcgg caacgtctag gatatacgag tactcgacgt atagcttgga
      901 gttagctaag ttaaacaaca ggaggataat tagaatgtta actaaagcag aaaaaacagc
      961 tattatgcaa aaatatgcta ctaaagaagg agatacagga tctccagaag tacaaatcgc
     1021 agtattaaca gcagatatca acaaattaaa tggacatttc aaacaacatc caaaagataa
     1081 tcattcaaat agaggattat taaaaaaagt tggtagaaga agagatttat taaaatattt
     1141 aaaaaataaa gatattgaaa gatattcttc tttatgtgaa agtttaggtt taagaaaata
     1201 attggacgct ttggcgtctt ttttctttac aaaaaagctg aattgaattc aactcagctt
     1261 ttaagagagg gttcaccctt attttgaata agctttttta atgataatca tgtagataac
     1321 caagaaataa ccaacaaaga gttgctgata aagaataaac ataaaaaacg ccatagtcaa
     1381 acattccgcc aataaattca ctaacaacaa ttaatgtaaa aatcattatc tttaaataat
     1441 tggaactttg ctgttttact ttaaataatt ttagaataga tttaaatgaa gcaataaaaa
     1501 atattataaa tagactcgct ccagcgattc cagttgttac aagagtttcc aaaaagacat
     1561 tatgactatg aacaacctgg atttgtttga caacattact gttggtcagg tgcctaccaa
     1621 tagtttgaaa attaccggca ccaattccta aaatagggtt ggtttgccaa atttcaattg
     1681 aagtattaaa taattcaata cgtccactgc tgatctgatt aatttgatct aaacttagct
     1741 gaaaatctcc ttgaaaaaac aattcaattg attcaattac tttattaatt tgaaaacggc
     1801 tgcttgtttc gtgatgatta ccacttaaat atgataggcc gttagcggtg atgatactag
     1861 taatgaaaat aatgatagaa agacctaaag aaaatagata tttctttaac tttgaataat
     1921 aacaatcttt aaaa
//
LOCUS       DAOMTA010000639         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2972_length_1933_cov_2.471778, whole genome shotgun sequence.
ACCESSION   DAOMTA010000639 DAOMTA010000000
VERSION     DAOMTA010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2972_length_1933_cov_2.471778"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..862
                     /locus_tag="PLK68_13925"
     CDS             179..862
                     /locus_tag="PLK68_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM91546.1"
                     /translation="MKIGILDDERSMLLRIRGYVEEARIKEPYTVELFQDVEEFFNCG
                     KNFDILLLDIDMPTMSGIQVAKKLLKQNTIIIYITNYENKMQEAFDINVAGYLLKSEL
                     ETKLTPTLLKAINRRQQNDDIWIKKKGEIFHFRSRNIIYIESVKRYLHFYTNDNNFYI
                     PYMTLEEVNQYLPDNFVQINKSQIININMVVSMNGYILKLKGIDESLEISRRRKKDVF
                     DLLMQEIKE"
     gene            862..>1933
                     /locus_tag="PLK68_13930"
     CDS             862..>1933
                     /locus_tag="PLK68_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRM91547.1"
                     /translation="MINFFIEYLYTLIYVVALSYVISLTYPYIRKENMLRNIMVILSY
                     TFVLNIVTYTSPHNGWLYVIGNLMCISLDFIYCGVLVKRFRLNNLFITTIYYSIQIIV
                     GSVMIAVALEIFDNTYLDIVWGSMRVVFPLGNYISSALICKYAVQKRDFVEHQLPKKF
                     LYSFCLINIVEVIIVLILNVLSVEQGDIYIFFILILAGVQMILVNNYIINSSRMYLRN
                     KELELANYSYATTRRHISELEKEQERLYKFKHDINNHLQVLEEVVETESVANQYLKAI
                     KEDLNAPVKLIRTGNIFVDACLNAKIRNNDEVNYVVDAVIDRNVNIAQDKLCSLLFNL
                     IDNATEAALKTAEKIVEIKIFSK"
BASE COUNT          711 a          203 c          342 g          677 t
ORIGIN      
        1 aggcaatttt agctcgtgat aaggatttag ctaaagaaaa aattcaaaat catattcgta
       61 aaacacaagt gctggtaaga aattactatg aaaaaaagca acaggataag gcctaagggc
      121 cttttttaat tagtcaaaaa aggttttatc aagtataata aataaatgag gtgtaaagat
      181 gaaaataggt atactagatg atgagcggtc aatgctactt agaatcagag ggtatgtaga
      241 ggaggctaga attaaggaac catatacagt tgaacttttt caagatgttg aagagttttt
      301 taattgtggt aaaaactttg atattctttt attagatatc gatatgccaa caatgagtgg
      361 aatacaggta gcaaaaaaat tattgaaaca aaatacgatc ataatatata taactaatta
      421 tgaaaataaa atgcaggagg cgtttgatat taatgtagca ggatatttac ttaaatcaga
      481 attagaaaca aagttaacgc caaccttgct taaagcgata aatcgtagac aacaaaatga
      541 tgatatctgg atcaagaaaa agggagagat ttttcatttt cgaagtagaa atattattta
      601 tatcgaatca gtaaaaagat acttacattt ttatactaat gataataatt tctatattcc
      661 ttatatgacg cttgaagaag ttaatcagta tttaccagat aattttgtcc aaataaataa
      721 aagtcagata atcaatatta atatggttgt ttcgatgaat ggatatattt taaaattaaa
      781 aggtattgat gaatcgctag aaattagtag aagaagaaaa aaagatgtat ttgatctttt
      841 aatgcaggag atcaaagagt gatgattaat ttttttattg agtatttata tacattgata
      901 tatgttgttg ctttaagtta tgttatttca ttaacctatc catatattcg taaagaaaac
      961 atgcttagaa atattatggt tatattaagt tatacgtttg ttttgaatat tgttacgtat
     1021 acttcaccac ataatggctg gttatatgta attggtaatt taatgtgtat tagtttggat
     1081 tttatttatt gtggggtatt ggttaagaga tttaggctta ataatttgtt tatcactaca
     1141 atttattatt caattcaaat tattgtcggc tcagttatga ttgcagtggc gctagaaatt
     1201 tttgacaata catatttgga tatcgtatgg gggagtatgc gagttgtttt tcctttagga
     1261 aactatatat catcagcatt gatatgcaaa tatgcggttc aaaaacgtga ttttgtggaa
     1321 catcagttac ctaaaaaatt tttatattcg ttttgtttga tcaatattgt tgaagtgatt
     1381 attgtactga ttttaaacgt attatcagtt gaacaggggg atatttatat cttttttata
     1441 ttgatcttag caggtgttca aatgattttg gtaaataatt atatcatcaa ttcttcccgt
     1501 atgtatttac gtaataagga gttagagtta gcaaattatt catatgctac aactcgccgt
     1561 catattagtg aattagaaaa agaacaagaa cgtctttata agtttaagca tgacattaat
     1621 aatcatttac aagtcctaga agaagtagtc gaaacagaat cagtcgctaa ccaatattta
     1681 aaagcaatta aagaagattt aaatgctcca gttaaattga tacgtacggg aaatattttt
     1741 gtagatgctt gtttaaatgc gaagattcgc aataacgatg aagttaatta tgttgtagat
     1801 gcagtgattg atcgcaatgt taatattgct caagacaaat tatgttctct attatttaat
     1861 ctgatagata atgctactga agctgcttta aaaacagcgg agaagatagt tgaaatcaaa
     1921 atttttagta aag
//
LOCUS       DAOMTA010000640         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2991_length_1924_cov_2.017657, whole genome shotgun sequence.
ACCESSION   DAOMTA010000640 DAOMTA010000000
VERSION     DAOMTA010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_1924_cov_2.017657"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLK68_13935"
     CDS             complement(<1..460)
                     /locus_tag="PLK68_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91548.1"
                     /translation="MKFPTNFLWGGATAANQYEGGWQEGGRGIAVHDMLTDGNQENPR
                     RIYCKADNGNITSIQAGECIPFGFHPFIKESEYYPSHNSTDFYQEDIKLMAEMGFKVY
                     RLSISWTRIFPHGDDKEPNEDGLRFYDAVIDELKKYNIEPLVTILHFDMPL"
     gene            complement(463..1794)
                     /locus_tag="PLK68_13940"
     CDS             complement(463..1794)
                     /locus_tag="PLK68_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM91549.1"
                     /translation="MKLSEKLQNGLLAFSAKIEANKYLGSIKDAFTMYVPFIIIGSFG
                     SMLNILVSGTSGLAQWFPWLIKLSPAFTAMNFVTISCMALPIAFLIGTKLAQREGLPT
                     LESGLIGLLAYLAVCPNAISTAVEGLKDPIIISGLGSGVIGAQGLFVSMLISIMAIEL
                     LKGLSKIDAIKIKMPDSVPTGIARSFNILIPIFIIIALFAICGCLFESFTGNYLNVWI
                     YNIIQIPLQALANTTIGIVVLSLVNQLFWFLGIHGGMVIEGIRGPLSAAGLADNIAAV
                     SAGHVATNILTRGFWTSFVVVGGGGITLSLLIAILLFSRREDHKAIAKFSIVPGICGI
                     NEPVVFGLPLVLNPIFAIPFICNSAIAALIAIFATNIGFLTCGIVDCPPGLPIFITGF
                     ISYGLHGIIIQAIILVVTFFVWVPFVSVSNKQAAIEDATEKSEKDKNMNGE"
BASE COUNT          680 a          385 c          248 g          611 t
ORIGIN      
        1 tcaatggcat atcaaaatga agaattgtca ctaaaggctc gatgttatat tttttcaatt
       61 catcaataac agcatcataa aatcttagac catcttcatt cggttctttg tcatcaccat
      121 gaggaaaaat acgtgtccat gaaatagata aacgatatac tttaaacccc atttctgcca
      181 ttaatttaat atcttcttga taaaaatctg tagaattatg acttggataa tattctgatt
      241 ccttaataaa tgggtgaaat ccaaatggaa tacattcccc agcctgaata cttgtaatat
      301 taccattatc agctttacaa taaattcttc taggattttc ttgatttcca tcagttaaca
      361 tatcgtgaac agcaatacct cttccccctt cttgccatcc cccctcatat tgatttgcag
      421 cagttgcgcc accccataaa aaatttgtag gaaatttcat aatcattctc cattcatatt
      481 tttatctttt tcagattttt ctgttgcatc ttcaattgcc gcctgtttat ttgatactga
      541 aacaaacgga acccagacaa agaaagttac aactaaaatg attgcctgga taataattcc
      601 atgtaatcca tagcttataa accctgtaat aaaaatcggt aacccaggag ggcaatccac
      661 aatgccacat gtcaaaaagc ctatattagt cgcaaaaata gcaattaacg cagcaatcgc
      721 agagttacaa ataaatggaa tagcaaaaat tggatttaat actaatggta agccaaaaac
      781 taccggttca ttaataccac aaatacctgg aacaattgaa aatttagcaa ttgctttatg
      841 atcttcacgt cttgaaaata ataatatagc tattaataat gacagtgtga ttccaccacc
      901 gcctacaaca acaaaacttg tccaaaatcc ccttgttaaa atatttgttg caacatgtcc
      961 tgctgaaacc gcagcaatat tatctgccaa tccagcagct gataatggac ctctaatccc
     1021 ttcgataacc attcctccat gaattccaag aaaccaaaat agttgattga caagagataa
     1081 taccacgatt ccaatcgttg tatttgctaa tgcttgtaat ggaatttgaa taatgttata
     1141 aatccataca tttaagtaat taccagtgaa cgattcgaat aaacatccac aaatagcaaa
     1201 caatgcaata ataataaaaa ttggaattaa aatattgaat gaacgtgcta ttcctgttgg
     1261 tactgaatca ggcatcttta ttttaatggc atctattttt gataatccct ttaataattc
     1321 tatagccatg attgatatga gcatagaaac aaataagccc tgggccccaa taactcctga
     1381 acccaaacca ctgattataa taggatcttt caatccctca acagccgtac taatggcatt
     1441 aggacatact gctaaatatg ctaataaccc tatcaatcca ctttctaaag ttggcaaccc
     1501 ttctctttgt gccaatttag taccgattaa aaaagcaata ggtaatgcca tacatgaaat
     1561 agtaacaaaa ttcattgctg taaaagctgg tgatagcttt attaaccatg gaaaccattg
     1621 tgctaaacca ctagtccccg aaactaaaat atttaacata cttccaaatg aaccaataat
     1681 aataaaaggt acatacatcg taaaggcatc cttgattgag ccaagatatt tatttgcttc
     1741 aatcttagca gagaatgcta ataaaccatt ttgtaatttt tcagataact tcattttatc
     1801 tcctctctcc ttttccaaaa aatataagtt ttcattcctg cagtaaacta ttttcttata
     1861 ttccttagct ataattataa aatgagttat tatggaactc catgctttta atttctattt
     1921 attt
//
LOCUS       DAOMTA010000641         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_2996_length_1921_cov_2.483387, whole genome shotgun sequence.
ACCESSION   DAOMTA010000641 DAOMTA010000000
VERSION     DAOMTA010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_1921_cov_2.483387"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLK68_13945"
     CDS             complement(<1..741)
                     /locus_tag="PLK68_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRM91550.1"
                     /translation="MVKQLLIRGDDLGYSEAVTLGMISAHRKGLVNSVALMVNMPYAK
                     EAIKLAKQCPNLCLGLHVNVTNGYALAPKEMIPSLVDEHGIFLSSTIRRAQIKNNEPL
                     FSEEDAYIEAKYQIEKYIELVGQLPEYIDLHVLEVEPLINAVVRIYHEYNVPVCYYGM
                     DLEQGIIDQTMKQYDYYEEHDHDFENMFIDGYYQIKEGLNILVTHPGFIDYDVATTSS
                     MLKERLYDYSLVTSEKLKQWLRDNEIEVI"
     gene            889..>1921
                     /locus_tag="PLK68_13950"
     CDS             889..>1921
                     /locus_tag="PLK68_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM91551.1"
                     /translation="MKDRFSLSFKAFYLIYLGAIGSFIPYINTYLEKNAGLSGSQIGL
                     ITAISLVLGVCVIPIWGVVGDKTRKYSALLKLSIMAALVVLYFYYKAAVYPAIVVCAI
                     ALEVSRLGTMPMADTLATNYCHKTGGNYGSIRGMGSLGYMLAGMAVGFLADLFGLDGA
                     MFATYAVLLILAFSISFGFPKDDGKKEDGEEVKVKKGSFKELLTNKNFLFILFLQLLM
                     STVVDSAMTYGGNHLTVTLNSGASAISWMTFATVLPEVAFLMIAIKVLNKTGFKKFYL
                     IACVSMMLRFGVYAFIPNQYAFLAISIVHCIGVAIATVASLTYIRNSVDPAVLGTAIT
                     LLNATLSIGK"
BASE COUNT          589 a          350 c          300 g          682 t
ORIGIN      
        1 gattacttct atttcattat cacgaagcca ctgttttaac ttttcacttg ttacgagtga
       61 ataatcatat aatctttctt ttaacatcga agaagtagtc gccacatcat aatcaataaa
      121 accaggatgt gttactaaaa tattcaaacc ttctttaatt tgataataac catcaatgaa
      181 catattttca aagtcatgat catgttcttc ataataatca tattgtttca ttgtctgatc
      241 aataatcccc tgctccaagt ccattccgta ataacatact ggaacattat attcatgata
      301 aattcttacc actgcattaa tcaatggttc tacttctaaa acgtgtaaat caatgtactc
      361 aggcaattgg ccaactaatt caatatattt ttcaatttga tacttagctt cgatgtaagc
      421 atcttcctca ctaaataatg gttcattatt ttttatttgt gctcgacgaa tcgtactcga
      481 taaaaatatc ccgtgttcat caactagact aggaatcatc tcttttggtg ctaatgcata
      541 tccattagtt acattaacat gtaatcctaa acacagattt ggacactgct ttgctaattt
      601 aattgcttct tttgcatacg gcatattaac catcaaagca acactattta ccaatccctt
      661 tcgatgtgct gaaatcatcc ccaatgtcac tgcttcgcta tatcctaaat catcaccacg
      721 tatcaataac tgctttacca taattatcct cttttctata ttttatataa ttatactata
      781 ccaaataaat agtctataca atttattttt tcatattcat gaaacattta tgatagtgct
      841 tatatttgcg gtataataaa agataactaa gggaggacaa cacactatat gaaagatcgt
      901 tttagtttat catttaaagc tttttattta atttatttgg gcgcgattgg ctcttttatt
      961 ccctacatta atacttattt agaaaaaaat gccggcttaa gcggttcaca aattggctta
     1021 attacagcca tcagtcttgt tttaggggta tgcgtgattc ctatttgggg ggtcgttggt
     1081 gataagacca gaaaatatag tgcactatta aagttatcaa ttatggctgc actagtagta
     1141 ctttacttct attataaagc cgctgtttat cctgctatcg tagtttgtgc aatcgcactt
     1201 gaagtatcac gactaggaac aatgccgatg gccgacacgc tagcaactaa ttactgccat
     1261 aaaactggtg gtaactatgg ttctattaga ggaatgggct cattagggta tatgctggca
     1321 ggaatggctg taggattttt agctgatctt tttggtcttg atggagcaat gtttgcaaca
     1381 tatgccgttt tattaatctt ggctttctcg atttccttcg gctttcctaa agatgatgga
     1441 aagaaagaag atggtgaaga agttaaagta aaaaaaggga gctttaaaga acttctaact
     1501 aataaaaact tcttatttat tctattttta caactattaa tgtcaacagt tgttgattct
     1561 gctatgacat atggcggaaa tcacttaact gttactttaa atagtggtgc ctcagcaata
     1621 agctggatga cttttgcaac agttttaccc gaagttgcat tcttgatgat tgcaatcaaa
     1681 gttcttaata aaaccggctt taaaaaattc tatttgattg cctgtgtatc aatgatgctt
     1741 cgctttgggg tatatgcttt tattcccaat caatatgctt ttttagcaat aagcatcgta
     1801 cattgcatcg gcgttgccat cgcaactgta gcaagtttaa cctatattcg aaattccgtt
     1861 gatcccgcag ttttaggaac cgctattaca ttacttaatg cgacactatc aattggtaaa
     1921 g
//
LOCUS       DAOMTA010000642         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3004_length_1916_cov_2.566362, whole genome shotgun sequence.
ACCESSION   DAOMTA010000642 DAOMTA010000000
VERSION     DAOMTA010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3004_length_1916_cov_2.566362"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..451)
                     /locus_tag="PLK68_13955"
     CDS             complement(92..451)
                     /locus_tag="PLK68_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91552.1"
                     /translation="MLERIGINLSLSFIYILLFRHLITPTVIVNTDFQVIILYFLLAL
                     LISIFLEAWHYLIIDLIEYPKVTLTVIAIGMIVCLFIKTNLYMLFCLGYVVIMMLTDF
                     YRTYKMQQALILFKNKH"
     gene            complement(457..957)
                     /locus_tag="PLK68_13960"
     CDS             complement(457..957)
                     /locus_tag="PLK68_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM91553.1"
                     /translation="MKLICNEKHRQKLETFFARYQELDIILVEQGIEYAGLHYTFTLN
                     NLKSVESDLNEMLERYLIGYLAEKIYRINYQDIVYIEGFSKEVYLNTQTCQYLCHYKL
                     YELEQLLDKYIFIRINRSIIVNINYIEYMLPELNSRYTLYMQNGIMLVLTRSYLKKFK
                     ERLEIR"
     gene            complement(954..1874)
                     /locus_tag="PLK68_13965"
     CDS             complement(954..1874)
                     /locus_tag="PLK68_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91554.1"
                     /translation="MNQGIDFNEFVFVKGLISFAMLYFVLKGIERISSDYIEIEKYQK
                     HIKNFIIINFILSFPILNILFVSLLWFSMIGIYGYIFYKACCITYHLNKINQYLSERY
                     TKILIKRVHPRKKTYWLIAIIALIMSIGGITFLSDPIMYRLEDRSVFTNYAIYAYRND
                     DVIIEYCSIWNESNNKTGTLKTTNTKTVAYPGIYIRDELIEISKQVSIKVNDNSVLPD
                     DTIDITSENNVLKKPDIHLKNTNFKILKAYLIQGNHEYQLYYNEKIKNNYISGLPCVI
                     PIKNDNELKIILYDENNKSKTLLYQLELKN"
BASE COUNT          700 a          309 c          201 g          706 t
ORIGIN      
        1 acaactacgc tcacgagaga ctttcaccct agatatatgg catgcccatc acacttaaaa
       61 actgttgact tttctacttt gccgtcaatt tttaatgttt gtttttaaat aaaatcagcg
      121 cctgctgcat cttataagta cgataaaaat cagtcaacat cataataact acatacccta
      181 aacaaaataa catgtataaa ttagttttta taaataaaca aacgatcatt ccaatagcta
      241 tcactgttaa tgttacttta ggatattcaa ttaaatcaat gattagataa tgccatgcct
      301 ctaaaaaaat actgattaaa agggccaaca aaaaatataa tataatcact tgaaaatcag
      361 tatttacaat cactgtagga gtaattagat gcctaaataa taagatataa ataaagctca
      421 acgatagatt tatgcctatt ctttctagca cttcaatcac cttatttcta acctttcttt
      481 aaatttcttc aaataacttc ttgttaggac tagcatgatt ccattttgca tatacaaggt
      541 ataacgagaa ttcagttcag gcagcatata ctcaatataa ttaatattta caattattga
      601 cctatttatc cgaataaaga tatatttatc taataattgc tctaattcat atagtttata
      661 atgacatagg tactgacatg tctgtgtgtt taaatagacc tctttagaaa atccttcaat
      721 atagacaata tcttgataat taatcctata tatcttctca gcaagataac caattaaata
      781 ccgttcgagc atctcattta aatcactttc aacacttttt aaattattga gtgtaaaagt
      841 ataatgtaac cctgcatatt ctattccttg ttccactaaa ataatatcta attcttgata
      901 acgagcaaaa aatgtttcta atttttgacg atgtttctca ttacatatta atttcaattt
      961 tttagctcca attgatataa caatgttttg cttttattat tttcatcata tagaattatt
     1021 tttagctcat tatcgttttt aattggtata acacatggta aaccagaaat ataattgttc
     1081 ttaatttttt cattataata cagctgatat tcatgatttc cctgaatcaa atatgctttc
     1141 agtattttaa aattagtatt ttttaaatga atgtctggtt tctttaatac attattttca
     1201 ctagtaatat cgatagtatc atccggcaat actgaattat cattaacttt aatactcact
     1261 tgtttagaaa tttcaatgag ttcatcacga atataaatac ctgggtatgc aacagtttta
     1321 gtattggtcg ttttaagtgt tcctgtttta ttattactct cattccaaat agaacaatat
     1381 tcaatgatca catcatcatt tcgataagca taaatcgcat aatttgtaaa aacacttcga
     1441 tcctctaaac gatacattat cggatcagat aaaaaagtta ttccacctat actcatgatc
     1501 aaagctatga ttgcaattag ccaatatgtt ttctttcgtg ggtgaactcg cttgattaaa
     1561 attttcgtat atcgctcact taaatactga tttatcttat ttaagtgata cgtaatgcag
     1621 caagccttat aaaatatata tccatagatt ccaatcatag aaaaccatag taacgaaacg
     1681 aataaaatat ttaatatcgg aaatgataaa ataaaattga taattataaa gtttttaata
     1741 tgtttttgat atttttcaat ttcaatataa tcgctactta ttctctcaat tccttttaat
     1801 acaaaatata acattgcaaa actaatcaat cctttaacaa aaacaaattc attaaaatca
     1861 ataccttggt tcaataaaaa ttactataca taaagctatt gttaaaatta atgcat
//
LOCUS       DAOMTA010000643         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3012_length_1914_cov_1.982249, whole genome shotgun sequence.
ACCESSION   DAOMTA010000643 DAOMTA010000000
VERSION     DAOMTA010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3012_length_1914_cov_1.982249"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>969)
                     /locus_tag="PLK68_13970"
     CDS             complement(<1..>969)
                     /locus_tag="PLK68_13970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91555.1"
                     /translation="STDLKPNKEEFKYALKNKRFIKIIKEGYKAFRIILKKRSLIRQC
                     AKVNDADIIISSTLAFDKAFSKHQKNKLLIAWEHCHPDRDPKYPKKVYRAVKKFDLFI
                     PASKSIYEFYKEYLTGPQCIYLPLCIDEIPRNRVSLNTNQITVMGRLSKEKAYDDMLR
                     VFKKVLEQNPDAKLNILGDGEERASLSILADQLAISNSVIFHGNTVGEAKNEIMNNTS
                     VFVTTSHYESFGLVLLEAMSYGIPCVSFDSAKGSLDIIEDGKDGFIIRDRNLDEMANK
                     IVELLNKTTKTLQNNAIRKAKRFSYQNVKKQWLKEFNNFNIHDLKT"
     assembly_gap    970..979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<980..1566)
                     /locus_tag="PLK68_13975"
     CDS             complement(<980..1566)
                     /locus_tag="PLK68_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91556.1"
                     /translation="MFKNKIKYRNSYYKGATTPVDCISGCFFGIKKALIDRIGFLDEG
                     TFLFYEEDILCAKAKNAGLHACVLNNVKVIHNHSITIDKNINHYRKLRVLKDSQMYYH
                     KTCFKHSAHKLKRLQRTANWACSFARLRTKKDIISQHNGPKQKISILSLHLQVGGIEK
                     AICSLANMLVDNYDVEIINVYKLCEPSFCIDERVNV"
     gene            complement(1632..>1914)
                     /locus_tag="PLK68_13980"
     CDS             complement(1632..>1914)
                     /locus_tag="PLK68_13980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91557.1"
                     /translation="AVDIICVVDNCSTDDSVKQLKKLKNIELIALDTNEGYAAGNNAG
                     LKYLYEQECDNYIISNPDIIIDKRNLMDFIAHMNADTNYSVQLLKNKGQ"
BASE COUNT          622 a          338 c          222 g          722 t
ORIGIN      
        1 ctagttttta aatcgtgaat attgaaatta ttaaattctt ttaaccactg cttttttaca
       61 ttttgataag aaaaacgttt agcttttctt atagcattat tttgcaatgt tttagttgtt
      121 ttgtttaaaa gttcaactat tttatttgcc atctcatcaa gattacgatc tctaataata
      181 aaaccatctt tcccatcttc aataatatct aaagaacctt tcgcactatc gaaagacaca
      241 catggaatac cataactcat tgcctccaat aagactagac caaaactttc ataatgtgat
      301 gttgtaacaa acacacttgt attattcata atctcatttt tagcttcacc tactgtattt
      361 ccatgaaaaa ttactgaatt actaatagct aattgatcag ccaatatact taatgaagct
      421 ctttcttcac catcccccaa aatattcaat ttagcatcag gattttgctc caataccttt
      481 ttaaataccc tcaacatatc atcataggct ttttctttgg atagtcgtcc cattactgta
      541 atctgatttg tatttaaaga aacacgattt cttgggatct catcgataca caatggtaaa
      601 taaatacact gaggtcctgt taagtattct ttataaaatt catagattga tttcgaggct
      661 ggaataaata agtcaaactt tttaacagca cgatatactt ttttaggata ttttggatca
      721 cgatctggat gacaatgctc ccatgcaatt aataatttgt ttttctgatg ttttgaaaat
      781 gctttatcaa aagcgagggt acttgaaata attatatcag catcatttac tttcgcacat
      841 tgcctaatta agctccgttt ctttaaaatt attctgaaag ctttgtaccc ttccttgatt
      901 attttaataa atcgcttatt ttttagagca tacttaaatt cttctttatt tggttttaag
      961 tctgttgatn nnnnnnnnna cattaacacg ttcatcaata caaaaagatg gctcacacaa
     1021 tttatataca tttataattt caacatcata attatccact aacatattag caagtgagca
     1081 aattgccttt tcaattcctc caacctgtaa atgtaatgat aatatagata ttttttgttt
     1141 tggaccatta tgttgactaa taatatcttt ttttgtcctt agccgagcaa agctacaagc
     1201 ccaattagcg gttctttgca acctctttag cttatgagca gaatgtttaa aacatgtttt
     1261 atggtaatac atttgagaat cttttaaaac tcttagctta cgataatgat taatattttt
     1321 atcaattgtt attgaatgat tatgaatcac cttaacatta tttaaaacac aagcatgcaa
     1381 tccagcattc ttagcttttg cacataaaat atcttcttca tagaaaagaa acgttccttc
     1441 gtctaagaaa cctattctat ctattaaggc tttttttatt ccaaaaaaac atcccgaaat
     1501 acaatctaca ggagttgttg ctcctttata atatgaattt cgatatttaa tcttattttt
     1561 gaacaaccta tttacaatag gataattatc atgaatatca tacgcaatcg ttctttgttt
     1621 ccaaccacga tttattgtcc cttgttcttc aatagttgga ccgaatagtt agtatctgca
     1681 ttcatatgtg caataaaatc cattaggttt cgtttatcaa taataatatc aggattagaa
     1741 ataatataat tatcgcactc ttgttcataa agatacttta atcctgcatt attaccagca
     1801 gcatatcctt cattggtatc taacgcaatt aattcaatat ttttcaattt ctttagttgt
     1861 tttactgaat catcagttga gcaattatca accacacaaa taatatctac agca
//
LOCUS       DAOMTA010000644         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3013_length_1913_cov_2.880517, whole genome shotgun sequence.
ACCESSION   DAOMTA010000644 DAOMTA010000000
VERSION     DAOMTA010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3013_length_1913_cov_2.880517"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLK68_13985"
     CDS             complement(<1..286)
                     /locus_tag="PLK68_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM91558.1"
                     /translation="MTMYIQTQNQYTQFQSDELTDLLGKISFTINKFGLWQSNHDTRA
                     NYITDDIEIVYYREGGSITTIGNKKYTCPPHSFLIIEPYKLNTSINTGNTN"
     gene            417..>1913
                     /locus_tag="PLK68_13990"
     CDS             417..>1913
                     /locus_tag="PLK68_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRM91559.1"
                     /translation="MLEQILHFKGTWRSYQQRVLDKYDRYSQDRKIHIVAAPGSGKTT
                     LGIELIKRIDYSALILVPSITIREQWVERICEAFLVKQENRDQYLSQDLKKPKLITVV
                     TYQALHSAMSHYCGELVETNDEFKTVEEVDYHNFDVISNFKECQLGTLCLDECHHLRS
                     EWWKSLEEFKKAFNNIFTVALTATPPYDSNLSMWTRYMDMCGDIDEEITVPELVKDGT
                     LCPHQDYVYFNYPASQEKQKLSIFEENSQSILEQLIQDEYFCKAIMSHRFFTEEVSDD
                     ELLDDPSYLSAMIIFLNTKGVCSANKYQKLLGYKSLEPLSLKWLEILLQGFLYDDIAS
                     YRVEEQYRDELIRELKSKGLIEKRKVSLCLNQAIEKMLINSVGKCESIKEIVNYEYKT
                     MKQELRLLILTDYIRKEYERALGDESKDVNNLGVLPFFEQLRRDSAKNKTAIKFGVLC
                     GTMIIIPKDAQTALIELVEEPSKISFHQIGKLDDYVKVEISGNRNFITG"
BASE COUNT          673 a          234 c          366 g          640 t
ORIGIN      
        1 agttagtatt tccagtattt attgaagtgt tgagcttata gggttcgatg atgagaaagc
       61 tgtggggtgg gcaggtgtat tttttattgc caatggttgt aatagaccca ccttctcgat
      121 aatagacaat ttcaatatca tcagtaatat aatttgctct agtatcgtga ttactttgcc
      181 aaagtccaaa tttattgata gtaaaactaa tcttaccaag taaatcagtt aactcatcac
      241 tttggaattg ggtatattga ttttgggttt gaatatacat cgtcataatt acaccacctt
      301 tgattattat tgtactactt ttttagtgga aggagaattt ttatcataaa attaagttga
      361 tttgtattga tgttttaagg ttgatatgta taaataaagg taataggagg gattggatgt
      421 tagaacaaat tttgcatttc aagggcactt ggcgcagtta tcaacaacgt gttcttgata
      481 aatatgaccg ttatagtcaa gatcgtaaaa ttcatattgt tgctgcacct ggttcaggaa
      541 aaacaacttt gggaattgaa ttaataaaaa gaatcgatta ctcggcttta atcttagtcc
      601 catcaataac gattcgtgaa caatgggtag agcgtatttg tgaagctttt ttagttaaac
      661 aagaaaatcg cgatcaatat ctttctcaag atttaaaaaa accaaaatta attactgtcg
      721 tgacatatca ggcactacat agtgcgatga gtcattattg tggtgaacta gtagagacta
      781 atgatgaatt taaaacagtt gaagaagttg attatcataa ttttgatgta atcagtaatt
      841 ttaaagaatg ccaattagga actttatgtc tagatgaatg tcatcatctt cgaagtgagt
      901 ggtggaaatc attagaagaa tttaaaaaag catttaataa tattttcaca gtggctctaa
      961 cagcaactcc gccatatgat tctaatctaa gcatgtggac taggtatatg gatatgtgtg
     1021 gtgatattga tgaagagata acagttccag aattagttaa agatggtact ttatgtccac
     1081 atcaagatta tgtttatttc aattatccgg cctctcaaga aaaacaaaaa ttaagtatct
     1141 ttgaggaaaa tagtcaaagt attttggagc agctaataca agatgaatat ttttgtaagg
     1201 ctattatgag tcatcgcttt tttactgagg aagtaagtga tgatgagcta ttagatgatc
     1261 cgagctattt atcagcaatg ataatttttt taaatactaa gggagtgtgc agtgcgaata
     1321 agtatcaaaa gcttttagga tataaatcgt tagaaccctt atcacttaaa tggttagaaa
     1381 ttttactgca aggcttttta tatgatgata ttgcttcata tcgagttgaa gaacaatacc
     1441 gtgatgaatt gattcgtgaa ttgaagtcta aaggtttaat cgaaaaacga aaagtttctt
     1501 tgtgtttaaa tcaagcaatt gaaaaaatgc taataaattc ggttggtaaa tgtgaaagca
     1561 ttaaagaaat tgttaattat gaatataaaa cgatgaaaca agaattacga cttttgattc
     1621 ttactgatta tattcgaaaa gaatacgaac gggcattagg tgatgaaagc aaagatgtga
     1681 ataatttagg cgttttacca ttttttgaac aactaaggcg tgatagtgct aaaaataaga
     1741 cagcaattaa atttggtgtt ttatgtggca caatgattat tatccccaag gatgctcaaa
     1801 cagcattaat agaattagtt gaagaaccta gtaaaatcag cttccatcaa attggaaaat
     1861 tagatgatta tgtaaaagtt gaaattagtg gaaaccgtaa ttttataaca ggg
//
LOCUS       DAOMTA010000645         1913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3014_length_1913_cov_2.246502, whole genome shotgun sequence.
ACCESSION   DAOMTA010000645 DAOMTA010000000
VERSION     DAOMTA010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_1913_cov_2.246502"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLK68_13995"
     CDS             complement(<1..764)
                     /locus_tag="PLK68_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HRM91560.1"
                     /translation="MALFESYERRIDQINAALAKFDIKSIEEAKAICDEKGIDVYEIV
                     KSTQPICFENACWAYTVGAAMAIKNGDVKAVDAAKTIGVGLQSFCIPGSVADDRKVGL
                     GHGNLGSMLLDEGTECFAFLAGHESFAAAEGAIKIAEKANKVRTKPLRVILNGLGKDA
                     AKIISRINGFTYVETQFDYFTGEVKVLSTTAYSDGPRAKVNCYGADDVREGVAIMHKE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECIEQGKKYFS"
     gene            complement(780..1472)
                     /locus_tag="PLK68_14000"
     CDS             complement(780..1472)
                     /locus_tag="PLK68_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91561.1"
                     /translation="MIYSHEVEQMCSVAQGASHGCAPIPEEGKWVYSKEIKDISGLTH
                     GIGWCAPQQGACKLTLNVKEGIIEEALIETIGCSGMTHSAAMASEALVGKTLLEGLNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEGGLPVGAGLEDLGKGLRSMTGTSYSTKAK
                     GPRYLELTEGYINRIALDANNEIIGYEFVNLGRMMDMVKAGKDANEAMKEATGTYGRF
                     DDAAKYIDPRKE"
BASE COUNT          623 a          366 c          278 g          646 t
ORIGIN      
        1 gagaaatatt ttttaccttg ttcaatacat tcttttttat atgttccagc aactggatgt
       61 tggaaacgag tagggttagt agagttacca gtaattgata catctacacc ttctttatgc
      121 atgatagcaa caccttctct aacgtcatca gcaccatagc agttaacttt agcacgagga
      181 ccatcagagt aagctgttgt agataatact tttacttcac ctgtaaaata atcgaattga
      241 gtttccacgt aagtgaatcc gttaattctt gagatgattt ttgcagcatc ttttcctaaa
      301 ccgtttaaga taactcttaa tggtttagtt ctaactttat tagctttttc agcgattttg
      361 attgcacctt cagcagcagc gaaagattca tgtccagcta agaaagcaaa acattcagta
      421 ccttcatcta ataacataga acctaagtta ccatgtccta aacctacttt acggtcatca
      481 gcaactgatc ctggaataca gaatgattgt aaaccaacac cgattgtttt tgcagcgtct
      541 acagctttaa catctccatt tttgattgcc attgcagcac cgacagtgta tgcccaacaa
      601 gcattttcaa aacagattgg ttgagttgat tttacgattt cgtaaacatc aattcctttt
      661 tcatcgcaga tagctttagc ttcttcgatt gatttgatat caaatttagc tagtgcagcg
      721 ttaatttgat caattcttct ttcataactt tcaaataatg ccattttcgt accctcctat
      781 tattcctttc ttggatcgat gtacttagca gcatcatcga atctaccata agttccagta
      841 gcttctttca ttgcttcatt agcatctttt ccagctttaa ccatatccat cattctacct
      901 aagttaacaa attcataacc aataatttca ttattagcat ctaaagcgat tctattaata
      961 taaccttcag ttaattcaag ataacgagga cctttagctt ttgttgaata agaagtacca
     1021 gtcatacttc ttaaaccttt tcctaagtct tctaatccag ctccaacagg taaaccacct
     1081 tcagagaatg cagattgagt acgtccataa acgatttgta agaataattc tctcattgca
     1141 gtattgattg catcgcaaac taagtcagta tttaaacctt ctaataaagt ttttcctact
     1201 aatgcttctg aagccatcgc tgcagagtga gtcatccctg aacatccgat agtttcaatt
     1261 aatgcttctt caatgatacc ttctttaacg tttaatgtta atttacaagc accttgttga
     1321 ggagcacacc aaccgatacc gtgagttaaa ccagagatat ctttaatttc tttagaatat
     1381 acccattttc cttcttcagg aattggagca catccatgtg aagcaccttg agcgacacta
     1441 cacatttgtt ctacttcgtg tgaataaatc attttttcta actcccttcg tgttttcgat
     1501 tataaatagc tagtcatgct atttaaggcg aactaaaatc acctgagtaa agtatagaat
     1561 aaagccgagt taaagtcaat gaaacatcaa cgattatcaa aataaattcg acaaaataat
     1621 cggtaaaaag aggagtttat gaataccttt taatataata ttattacact aataaaaaca
     1681 tattgaacag gctatgagta tgaaaatacc catgtttaat catcgtaaag atatataggg
     1741 ataacactaa gatgattttt attgaaatat gggggagaaa ttaaactact caaagaacct
     1801 ttgactaaag aaaattattt ataatagcaa tttttgaagc aattagcatt gtcgaaaatt
     1861 caatcttgaa tcatgaatta ttaatcttat taaaaaggaa tggtgttaaa ttc
//
LOCUS       DAOMTA010000646         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3029_length_1905_cov_2.423243, whole genome shotgun sequence.
ACCESSION   DAOMTA010000646 DAOMTA010000000
VERSION     DAOMTA010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_1905_cov_2.423243"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1376
                     /locus_tag="PLK68_14005"
     CDS             18..1376
                     /locus_tag="PLK68_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM91562.1"
                     /translation="MNFIEKFNEIANKTLVPIANKLGNQRHLAAIRDGMVVAIPLSIL
                     GGFCLIISTPPFTPKSLPDWGVISDLLIGWYNWAQANATALRLPYNMTMALMGLFVAI
                     AIAYHLAKRYEMSGLNAAIVSTTVFLLICAPTTTAVLTSAISEGANTNDLLAQAASFI
                     PTTYLDAKGIFTAIIVSIGCVEIMRVMLKKNIRFKMPEGVPPAIASSFDSILPLFVCM
                     GVFYGLSLIIQNISGELLPSLIMTILAPAISGLDSLLGICLITIIAQTFWFFGLHGAS
                     ITQPIRLPFMQMYLMANITAFSAGEPIVNFFTQPFWSYVITLGGGGATLGLCILLLRS
                     KSVELKTLGKLSIGPAIFNINEPIIFGLPMVLNPLMMIPFIFVPVINTIIAYTCMALN
                     IVGKGVVETPWTTPAPIGAALGCMDIKAGIMVIILIILDMLLYYPFFKLMEKQKLAEE
                     NS"
     gene            1476..>1905
                     /locus_tag="PLK68_14010"
     CDS             1476..>1905
                     /locus_tag="PLK68_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRM91563.1"
                     /translation="MSKKRDNKRKVEIKGYDIAKLRFQRFLAMVIDWYISNMIVAIPV
                     TFFLRGKDYIQPYSFQLETYGYKIGMIYGLFVVVVGICYYFAVPTYIWKGQTLGKKIC
                     KLQVVKTDGQKVDTKTMFLREIIGAVVIEGGIVVSATYIRK"
BASE COUNT          593 a          275 c          377 g          660 t
ORIGIN      
        1 aagggaggaa ttaaaatatg aattttattg aaaaattcaa tgagattgcc aataagactt
       61 tagttccaat tgctaataaa cttgggaacc agcgtcattt ggcagcaatt cgtgatggta
      121 tggtagtggc gattccatta tcgattttag gtggtttttg tttaattatt tcaactccac
      181 catttacgcc aaagagttta ccggattggg gagtaattag tgatctatta attggctggt
      241 ataattgggc tcaggccaat gcaacggctt tgcgactgcc gtacaatatg acaatggcat
      301 taatggggct atttgttgct atcgcgatcg catatcattt ggctaaaaga tatgaaatgt
      361 caggattaaa tgcagcgatt gtttcaacca cagtattttt attgatttgt gcaccaacaa
      421 caactgcggt attgactagt gcaattagtg agggggccaa cactaatgat ctattagcac
      481 aggcagctag ttttattcct acgacctacc tagatgctaa aggtattttt actgctatta
      541 ttgtttctat cggctgtgtt gaaataatga gggtgatgtt gaagaaaaat attcgtttta
      601 agatgcctga gggagttcct ccagcaattg cttcatcatt tgattcaatt ttaccactat
      661 ttgtttgtat gggagtcttc tatggcttat cattgattat tcaaaatatt agtggtgaat
      721 tattacctag tttgatcatg acgattttag caccagcaat ttcaggatta gattcattac
      781 ttggaatttg tttaattaca attattgctc aaactttctg gttctttggt ttacatggtg
      841 caagtattac gcagccaatc agactgccgt ttatgcaaat gtatttaatg gcaaatatca
      901 ctgctttttc agctggtgag ccaattgtca atttctttac tcaaccattt tggtcttatg
      961 taattacact tggtggcgga ggtgcaacat taggtttatg tattctcttg ttacgctcaa
     1021 aatcagtaga gttaaagaca ttaggtaaat tatcaatcgg accagcaatt tttaatatca
     1081 atgagccaat catcttcggg ttaccaatgg tcttgaatcc gttaatgatg ataccattta
     1141 tttttgtacc ggtaattaat acaattattg cttatacttg tatggcttta aatattgttg
     1201 gaaagggcgt agttgagact ccatggacaa ctccggcacc gattggcgca gccttgggct
     1261 gtatggatat caaagcggga atcatggtta ttatcttaat tattttggat atgctacttt
     1321 attatccatt ctttaaatta atggaaaaac agaaattagc tgaagaaaat agctaattta
     1381 ttaaaagttg atacaatcaa ggataaagat aaacttacac accaaaaaga gattttaaat
     1441 ctctttttat tttaaaaatt tattggagaa gaaaaatgag taaaaaaaga gataataaaa
     1501 gaaaagtaga gataaaaggc tacgatattg ctaagttgcg atttcagcgt tttttagcaa
     1561 tggtcattga ttggtacatt agtaatatga ttgtcgctat tcctgtaact ttttttctta
     1621 ggggtaagga ctatattcaa ccatattctt ttcaactaga aacatatggc tataaaatag
     1681 gaatgatcta tggtctgttt gttgtagttg ttgggatttg ttattatttt gctgttccga
     1741 catatatatg gaaaggtcaa actttaggaa agaaaatttg taaactacaa gttgttaaaa
     1801 ctgatggaca aaaagtggat acaaaaacaa tgtttttaag agaaatcatt ggtgcagtag
     1861 taatagaagg tgggattgtc gtttcagcca cgtatatccg taaat
//
LOCUS       DAOMTA010000647         1903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3034_length_1903_cov_2.094697, whole genome shotgun sequence.
ACCESSION   DAOMTA010000647 DAOMTA010000000
VERSION     DAOMTA010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_1903_cov_2.094697"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLK68_14015"
     CDS             complement(<1..750)
                     /locus_tag="PLK68_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM91564.1"
                     /translation="MKKYNVVIVGGGSHRNPDLMAMLASKKDVFPLKRVVLYDNEAER
                     QEIMGQYGEILMREYYPELEEFIYTTDPDVAFKDVDFALMQIRAGRLPMREKDEKIPL
                     AHGCVGQETCGAGGFAYGLRSVPAIIELVKQIRKHSPEAWILNYSNPAAIVAEATKRI
                     FPDDYRILNICDMPIAIMDAYAKLLGCQRKDFEPRYFGLNHFGWFTHLYDKKTGADLM
                     PSVREQLMAGEIFKKGSEDEHVREKSWVDTYN"
     gene            complement(901..1872)
                     /locus_tag="PLK68_14020"
     CDS             complement(901..1872)
                     /locus_tag="PLK68_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HRM91565.1"
                     /translation="MKRLSKILLVFLMVLGLTACSGGSKENEPAKIEMGKENKFDDLV
                     SYKIETISRPQQITPDVIGSFYTYYKPSKSTNVLIDVTMYMTNLQKKEMKLSSTLKGT
                     FVIDKTDYVASTAMVSEDGKTISQGGSLAAEGTNKVHFYAEVKPSLLKNNIEFKLTTV
                     DEENPKEANMSFKLTDIAKNYESKNLNDTIVLDGRGEIALQAVNITKKLEPANPVGLY
                     TYYQVQNDGNSFVVLTTSIKNISESDITASNIAVAKLVDKDSNEYPANSFYEKDDRSN
                     LASASTTVLTPGQSGMIHFVFEVSDAVANGEKSVRITYNGKVFIVNL"
BASE COUNT          606 a          400 c          223 g          674 t
ORIGIN      
        1 attataagta tctacccatg atttttcgcg aacatgttca tcttctgaac cttttttaaa
       61 aatttctcca gccattagtt gctcccgaac agaaggcatt aaatctgctc ccgtcttctt
      121 atcatataaa tgagtaaacc atccaaaatg gtttagtcca aaatatcgtg gttcaaaatc
      181 tttacgttga catcctaata attttgcata agcatccata attgcaattg gcatatcaca
      241 aatgtttaag atacgataat catcagggaa aattcgtttt gttgcttccg ctacaattgc
      301 tgctggattt gaataattta aaatccatgc ttctggagaa tgtttacgaa tttgtttaac
      361 taactcaata attgcaggga cactgcgtaa tccataggca aaaccacctg ccccacatgt
      421 ttcctgacca acacatccat gtgctaatgg gattttttca tccttttcac gcattggcaa
      481 acgtcccgct ctaatttgca ttaaagcaaa atctacatct ttaaaagcaa catcaggatc
      541 agtagtgtaa ataaattctt ccaattcagg ataatattcc ctcattaaaa tttcaccata
      601 ttgtcccatg atttcttgcc gttcagcttc attatcatat agaactactc gctttaaagg
      661 aaatacatct tttttacttg caagcatggc cattaaatct ggatttcggt gactaccacc
      721 accaacaatt actacattat attttttcat ctcaaatacc ttctttctat gactttactt
      781 tagcacttgc tttaaggtct cacaatactt tcaaagctaa tcgtgcaaag accatatact
      841 taggtattct tcacaaaaaa gcgtgacttt tcacaaaaaa agatctagca atactagatc
      901 ttaaagatta acaataaata cttttccatt atatgtgatt ctaactgatt tctccccatt
      961 tgcaacagca tcactaactt caaaaacaaa atgaatcatc ccactttgac ccggagtcaa
     1021 tacagtagtg ctggcactag ctaaattact acgatcatct ttttcataaa atgaatttgc
     1081 tggatattca ttactatctt tatcaaccaa tttagctaca gcaatattag atgctgtgat
     1141 gtcactttct gaaatatttt ttatcgaagt agtcaaaaca acaaaactat ttccatcatt
     1201 ttgaacttga taataagtat ataaaccaac cggattagca ggctccagct tttttgtaat
     1261 atttactgct tgtaaagcaa tttcaccgcg accatctaat acaatcgtat catttaaatt
     1321 cttactttca taatttttag caatatccgt taatttaaaa ctcatattcg cttctttagg
     1381 attttcttca tcaacagtag taagcttaaa ttctatatta ttttttaaca aacttggttt
     1441 aacttcggca taaaaatgca ctttattagt cccctcagca gctaaacttc caccttgact
     1501 aattgttttt ccatcttcac ttaccattgc tgttgaagcg acataatcag ttttatcaat
     1561 aacaaatgtt cccttcaacg tactagatag cttcatttcc tttttttgta gattagtcat
     1621 atacatagtc acatcaatta atacatttgt tgacttagat ggtttatagt atgtataaaa
     1681 acttccaatt acatcagggg taatttgctg tggtctacta atcgtttcaa ttttatatga
     1741 aactaaatca tcaaacttat tttcttttcc catttcaatt tttgccggct cattctcttt
     1801 actcccacca ctacacgcag taagacctaa aaccatcaaa aatactaata aaattttact
     1861 caatcttttc attatatcga cttcctccct ttaatacctt aat
//
LOCUS       DAOMTA010000648         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3035_length_1902_cov_1.930698, whole genome shotgun sequence.
ACCESSION   DAOMTA010000648 DAOMTA010000000
VERSION     DAOMTA010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3035_length_1902_cov_1.930698"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLK68_14025"
     CDS             <1..730
                     /locus_tag="PLK68_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM91566.1"
                     /translation="LDFVVEKAVNNVGVDINTASVSLLQYVAGLNSGVAKNIIKYRDE
                     NGKFTNRKEIMNVSKLGAKTYQQAIGFLRIPGANDPFDATAIHPENYQQAKKLLKLID
                     ADASLLGTNEIADKLSNINKEEVMNELNIDNYTLDDIIDSFIKPNRSPRDAYATPLLK
                     KDILKIEDLKPGMQLEGTVRNVVDFGAFVDIGLKNDGLVHISKISHERIKHPLDKLSI
                     GDIVEVYVIDVDVNKHRVGLSMIK"
     gene            742..>1443
                     /locus_tag="PLK68_14030"
     CDS             742..>1443
                     /locus_tag="PLK68_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRM91567.1"
                     /translation="MKKFLVGIVIIIIASIGFNLKPDKRDQAVVVQEEPVVKKEEVIT
                     YKELLEYLDIFKTGDIINIENTNYLSSYAIPGRWKHTIFYLGTYQQFSQVFTPEDKYY
                     EEINKHYKNQDEVLVLDSNSTGVKIRTFDQMANLKEESYLKALSGYRFNEDDNFIKTY
                     LSRALDYLGTPYDYSMTTYDDKALYCSELVYYALLANNIEVTKTSSIVEHVVITPTDL
                     SDFLETLEDIDHVYLL"
     assembly_gap    1444..1465
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1542..>1902
                     /locus_tag="PLK68_14035"
     CDS             1542..>1902
                     /locus_tag="PLK68_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM91568.1"
                     /translation="MKISTKGRYAIRVILDIAMHDDGKYIPLKDIAKRQDLTVKYLEQ
                     IISLLNKAGYLQSLRGNAGGYRLSKKPEECIIGDILRITEGDLAPIPCLKDEINNCSR
                     ANECITLPFWQGLDKVIK"
BASE COUNT          697 a          221 c          343 g          619 t
ORIGIN      
        1 attagatttt gttgttgaaa aagcggtaaa taatgttgga gttgatatca atactgcttc
       61 agtatcatta ttacaatatg ttgctgggtt aaatagcgga gtggccaaaa atattattaa
      121 atatcgtgat gaaaatggta aatttacaaa tcgtaaagaa attatgaatg ttagtaaatt
      181 aggtgcaaaa acatatcagc aggcaatcgg atttttgcga attcctggag ctaatgatcc
      241 atttgatgca acagcaattc atccggaaaa ctatcaacaa gcaaaaaaat tacttaaatt
      301 aatagatgct gatgctagtt tattaggaac aaatgaaatt gctgataaat tatcaaatat
      361 taacaaagag gaagtaatga atgaactaaa tatcgacaat tatacgttag atgatattat
      421 tgatagtttt ataaagccaa atcgctcacc ccgtgatgca tatgcaacac cattattaaa
      481 gaaagatatt ttaaaaattg aagatttaaa acccggtatg caactagaag gaacagttag
      541 gaatgttgtt gattttggag cttttgtcga tattgggtta aaaaatgatg gattagtcca
      601 tatttcgaag atttctcatg aaagaattaa gcatcctttg gataaactaa gtataggtga
      661 tattgttgaa gtctatgtca ttgatgtaga tgtcaacaaa catcgtgttg gattaagtat
      721 gattaaataa aggatgagtt gatgaaaaag tttctagttg gaatagttat tataataatt
      781 gccagtattg gctttaattt aaaacctgat aaaagagatc aagctgttgt ggttcaagaa
      841 gagcctgtag ttaaaaaaga agaagttatt acttataaag aactattaga atatcttgat
      901 atttttaaaa caggagatat tattaatatt gaaaatacta attatttaag ttcatatgct
      961 attccaggaa gatggaaaca tactattttc tatctaggaa catatcagca gtttagtcag
     1021 gtttttacac ctgaagataa atattatgag gaaattaata aacattataa gaatcaagat
     1081 gaggttttag tactcgacag taattctacg ggtgttaaaa tacggacatt tgatcaaatg
     1141 gctaatctta aagaagaatc gtatttaaaa gcactttccg gctatcgttt taatgaggat
     1201 gataatttta ttaagacata tttaagtcgg gcgttagatt atttgggaac accgtatgat
     1261 tacagcatga caacttatga tgataaagct ttatattgta gtgaattagt ttattatgca
     1321 ctactagcta ataatataga agtgactaaa acgagcagta ttgtcgaaca tgttgtaatt
     1381 accccaacag atttaagtga ttttttagaa acattagaag atattgacca tgtatatttg
     1441 cttnnnnnnn nnnnnnnnnn nnnnnataaa tgactagttt ttgctttatt ttacttgtat
     1501 tcctacttta atactagtat tatataaacg aggtgaacat catgaaaatt tcaaccaaag
     1561 ggcgttatgc aattagagtt atccttgata ttgcgatgca cgatgatggt aaatatattc
     1621 cactgaaaga tattgctaaa agacaagatc tgacggttaa gtatttagaa caaataattt
     1681 cattactaaa taaagcgggc tatttacaaa gtcttcgtgg taatgctgga ggttatcgct
     1741 tatcaaaaaa acctgaagaa tgtataattg gtgatattct aagaattaca gaaggtgatt
     1801 tagctccaat tccctgcttg aaagatgaaa ttaataattg cagtcgtgcc aatgaatgta
     1861 ttacattacc gttttggcaa ggattagata aagtgattaa ag
//
LOCUS       DAOMTA010000649         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3043_length_1899_cov_3.133406, whole genome shotgun sequence.
ACCESSION   DAOMTA010000649 DAOMTA010000000
VERSION     DAOMTA010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3043_length_1899_cov_3.133406"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>498)
                     /locus_tag="PLK68_14040"
     CDS             complement(<1..>498)
                     /locus_tag="PLK68_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM91569.1"
                     /translation="KDEWVKLEIKGYANKAELYVNGELVDTLAKNATGGKYATLTLPL
                     ERIGSKTTSFKGIIDNLEISTTGSQSDDTDYTKVDSSNFTVSTDNENSQAGNEGPITY
                     AFDNNEVTFWHSNYSPYQALPATVEIDMKEVHTINQFDYLPRQDGNTNGQITKYELYI
                     KENAA"
     assembly_gap    499..508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<509..>1899)
                     /locus_tag="PLK68_14045"
     CDS             complement(<509..>1899)
                     /locus_tag="PLK68_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRM91570.1"
                     /translation="KMNDFQVHLNDNYIWVEDYGDNAFDAYSGFRLESDIKAGGNGGL
                     NQADLTSKDVFYTKDQFRTFIQECRDMGVAVVPEFDTPAHSLALTKVRPDLAMKDKSV
                     ARHWDHLDLDSMYDESLAFTQSIFNEYMNGDDPVFDQNTTVNVGTDEYDGKYAENFRQ
                     YTDDMLKFIQDTGRDVRLWGSLSMRKGSTPVRSENVQMNIWNTSWANPNEMYKQGFDL
                     INMVDGTLYMVPGAGYYNDYLNSQNIYNNWQPNNMGGTIIPAGDEQMLGSAYAIWNDM
                     VDKKANGISEYDIYDRFEKALPAMSSKLWGDGQDLKYNELNEVVNSLGTAPNSNPRDV
                     VTSKSDTVLNYDFNKSEIVDKSGNEYNVVNKKNVATVAGKFSKGLELSGGESYIETPL
                     ADMGPNNSVSFWVKMDKDATGEQVLFESDKGSIKMSQKDTGKVGFSRIGYDYSFNYEL
                     PKDIILGKKISTL"
BASE COUNT          583 a          393 c          237 g          676 t
ORIGIN      
        1 ctgccgcatt ttctttaata tataattcat atttagtaat ttgcccattg gtattaccat
       61 cttgtcgtgg tagataatca aattgattaa tagtatgaac ttctttcata tcaatctcta
      121 cagttgctgg taaagcttga tatggggaat aattactatg ccagaaagta acctcattgt
      181 tatcaaatgc ataagtaatc ggtccttcat tacctgcttg agaattttca ttatctgtag
      241 atacagtaaa gttactgcta tctacctttg tataatcagt gtcatcactt tgactaccag
      301 tagttgaaat ctctaaatta tcaataatcc ccttgaatga tgttgtctta cttccaattc
      361 tttctaatgg taaagttaac gttgcgtatt taccaccagt tgcattttta gctaaagtat
      421 ctactaattc accattaaca tacaattcgg ccttattagc atatcccttt atttctaatt
      481 ttacccattc gtctttagnn nnnnnnnnaa taaagtggat atcttcttac ctaaaataat
      541 atctttaggt aattcataat taaatgaata atcataaccg attcttgaga agcctacttt
      601 tccagtatct ttttgtgaca ttttaattga acccttatct gattcaaata aaacttgttc
      661 accagttgcg tctttatcca ttttcaccca gaaagataca ctgttattcg gtcccatatc
      721 tgctaatggt gtttcaatat aactttcacc accacttaat tctaggcctt tagaaaattt
      781 tcctgctaca gttgcaacat tctttttatt aacgacatta tactcgtttc ctgatttatc
      841 aacgatttca ctcttattaa aatcatagtt taatactgta tcgcttttgc tcgttacaac
      901 atcacgcgga tttgaatttg gggcagttcc taatgaatta actacttcat ttaattcatt
      961 atattttaaa tcttgaccat cgccccataa tttagatgac attgccggta aagctttttc
     1021 aaaacgatca taaatatcat attcacttat accattcgcc tttttatcaa ccatatcatt
     1081 ccaaattgca tatgcagaac ctaacatttg ctcatctccg gccggaatga tcgttccacc
     1141 catattatta ggctgccagt tattataaat attttgacta tttaaataat cgttataata
     1201 gcctgccccc ggcaccatat ataaagttcc atcaaccata ttaattaaat caaaaccttg
     1261 tttatacatt tcattggggt tagcccaact tgtattccaa atgttcattt gcacattttc
     1321 agaacgaact ggtgtagaac ctttgcgcat tgataaactt ccccacaaac gaacatcgcg
     1381 tcctgtatct tgaataaatt ttaacatatc atcagtatat tgacggaagt tttctgcgta
     1441 cttaccatca tattcatcag tacctacatt aactgttgta ttttgatcaa aaaccggatc
     1501 atcaccattc atatattcat taaaaattga ttgagtaaaa gctaaactct catcatacat
     1561 actatctaga tccaaatgat cccagtgacg tgctacagat ttatctttca tcgctaagtc
     1621 tggacgaacc ttcgttaaag ctagagaatg agctggcgta tcaaattcag gaacaaccgc
     1681 tacccccata tcccgacatt cttggataaa tgtccggaac tgatcttttg tataaaaaac
     1741 atctttgcta gttagatcag cttgatttaa tccaccatta ccacccgctt ttatatctga
     1801 ttctagtcta aacccagaat aagcatcaaa agcattatca ccataatctt caacccaaat
     1861 atagttatca tttaaatgta cttggaaatc attcatttt
//
LOCUS       DAOMTA010000650         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3051_length_1898_cov_2.153011, whole genome shotgun sequence.
ACCESSION   DAOMTA010000650 DAOMTA010000000
VERSION     DAOMTA010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_1898_cov_2.153011"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1898
                     /locus_tag="PLK68_14050"
     CDS             <1..>1898
                     /locus_tag="PLK68_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRM91571.1"
                     /translation="IGDYEIHPKYGKQFKLSEYRIIYAKESEEIIKYLSSPLFKGIGK
                     ALATQIVNTLGEECLEKIKEDKHNLDLVRGMTEKRREIIYEALTNGDYDQEVMQFFMG
                     HGISLKNLGLIQAYYQEKTLEILQNNPYQLVEDIDGIGFKTADELALKTGGTLDNPNR
                     IKAGIIYSIKQYGFNTGSTYCYLDEIKIMFSKIIYNIEEVSFNEYLDELIDEGLIIQR
                     GDKYYYFEMDEAEKNIAEYLKIRINKPDELFDEKEVERLLTNYEKTQGICYAAKQKEA
                     LNYFLKSSCMILTGGPGTGKTTIVQALLKVYSVLYPDDRIGLVAPTGRAAKRLTELTG
                     IYACTIHRLLKWDLHSNTFAMNKSNPLDLDVLIIDEFSMVDCLLLSKLFEAGRGINKV
                     LFIGDYHQLPSVAPGNILQDLMEAGVKTIELDEIFRQAKDSGIIQLAHHIIHNEIENM
                     DLFEQYRDINFFPCINYDVVKNVKIIVKKAIDEGYDTNDIQVLVPMYQGVAGIDALND
                     ALQDVFNPIDEFNDSYQIGRKEYRVGDKILQLKNRPDDDVFNGDIGTLVEICRKDNFE
                     YLQDTLIVDFDGNFVEYTSNTFNTITHAYCMSIHKSQGNEFKIVIMAVLSDYYIMLRR
                     NLLYTAIT"
BASE COUNT          674 a          223 c          378 g          623 t
ORIGIN      
        1 ttattggtga ttacgaaatt catcctaaat atggtaaaca gtttaaatta tcagaatatc
       61 gtatcattta tgcaaaagaa agtgaagaaa ttattaaata tttatctagt ccgctattta
      121 aggggattgg taaagctttg gcaacacaaa tagtaaatac acttggtgaa gagtgtcttg
      181 aaaagattaa agaagataaa cataatttag atttggttag aggaatgact gaaaaacggc
      241 gggaaataat ttatgaagca ttgactaatg gtgattatga tcaagaagta atgcagtttt
      301 ttatgggaca tggtatcagt ctgaaaaact tgggcttgat tcaagcatat tatcaagaaa
      361 aaacattaga aatacttcaa aacaatccgt accaactagt agaggatatt gatgggattg
      421 gatttaaaac agctgatgag ttagctttaa aaacaggtgg gacacttgat aatcctaatc
      481 gaattaaagc tgggattatt tacagtatta agcaatatgg gtttaatact gggagtacat
      541 actgttacct tgatgaaatc aaaataatgt tttctaaaat aatatataat attgaagaag
      601 tatcgtttaa tgaatatctt gatgaattga ttgatgaagg attgattatt caacgtggtg
      661 ataagtatta ttattttgaa atggatgagg ctgaaaaaaa tatagctgaa tatttaaaaa
      721 tacggataaa taaacccgat gaactttttg atgaaaaaga agtagaacga ttgttgacaa
      781 attatgaaaa gactcaaggt atatgttatg ctgccaagca gaaagaagcc ctaaattatt
      841 ttttaaagtc ttcttgcatg attttgacag gcggacctgg aactgggaaa acgacaattg
      901 ttcaagcact tttaaaagtg tattcggtat tgtacccaga tgatcgaatt ggtttagttg
      961 cgccaactgg tcgggctgca aaacgattga cagaattaac tggaatttat gcttgtacga
     1021 ttcatcgcct attaaaatgg gatcttcata gtaacacatt tgctatgaat aaaagcaatc
     1081 cattagattt agatgttttg atcattgatg agttttcaat ggtcgattgt ttattgttga
     1141 gtaaactttt tgaggcgggt cgaggaatca ataaagtatt atttatcggt gattaccatc
     1201 aattgccttc ggttgcacct ggaaatatat tacaggattt gatggaagct ggagtgaaaa
     1261 cgattgagct agatgaaatc ttcagacaag ctaaggattc aggaattatt caattggctc
     1321 atcatattat tcataatgaa attgaaaata tggatctttt tgagcaatat cgggatataa
     1381 acttttttcc ttgcataaac tatgatgtcg taaaaaatgt taagataata gtaaaaaaag
     1441 caattgacga aggttatgac acaaatgata ttcaagtact agtaccaatg tatcagggag
     1501 tggctggaat cgatgctttg aatgatgctt tacaagatgt ttttaatccg attgatgaat
     1561 ttaatgattc atatcaaatt ggccgcaagg aatatcgtgt aggtgacaaa attttgcagt
     1621 taaaaaatcg acctgatgat gatgttttta atggtgatat tggaacgctt gttgaaatat
     1681 gtcgaaaaga taattttgaa tatttgcaag atacactgat tgttgatttt gatggtaatt
     1741 ttgttgaata tacaagtaat acttttaaca caattactca tgcttattgt atgtcgattc
     1801 ataaatcgca gggaaatgaa tttaaaatcg ttattatggc ggtcttgtct gattattata
     1861 ttatgctacg gcgtaatcta ttatatacag cgattaca
//
LOCUS       DAOMTA010000651         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3053_length_1898_cov_1.641888, whole genome shotgun sequence.
ACCESSION   DAOMTA010000651 DAOMTA010000000
VERSION     DAOMTA010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_1898_cov_1.641888"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..888
                     /locus_tag="PLK68_14055"
                     /pseudo
     CDS             1..888
                     /locus_tag="PLK68_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608947.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
     assembly_gap    923..932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <933..1064
                     /locus_tag="PLK68_14060"
     CDS             <933..1064
                     /locus_tag="PLK68_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91572.1"
                     /translation="GGDISRKKKLLEKQKEGKKRMKAVGNVEIPQEAFMAILSVDDE"
     gene            1154..>1898
                     /locus_tag="PLK68_14065"
     CDS             1154..>1898
                     /locus_tag="PLK68_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91573.1"
                     /translation="MTIKLPDNFFFGAALSGPQTEGAYNVGGRLRSYWDMWSDQEINA
                     FHNNVGSYVGNDMYHRYEEDIKLFKSMNFKSFRTSMQWTRLLDQDGNINPEGAAWYHK
                     LIDCANENGIDIFMNMYHFDMPEYLYKRGGWANREVVEAYANFVKKAMEEFGGKVKYW
                     FTFNEPIVEPEQQYLHGVWFPYEKNIKRSLDVQYNITLAHCLAVMNFRELQARGKMVE
                     GAKIGMINCFAPPYTKEDPSPEDLEAVRMT"
BASE COUNT          646 a          280 c          390 g          572 t
ORIGIN      
        1 acgatcacag tcgcatccaa tcctgcaagt gaaccactat cgggatatcg tgaaatgcat
       61 ccaatggtat attgtggttt atatcctgtt gataacgctc gttataaaga tttacgggaa
      121 gcgttagaaa aaatgaaatt aagtgattca tcattaatgt ttgatccaga aacctcacaa
      181 gcattaggat ttggatttag atgtggtttt ttaggcttgt tgcatatgga tgttattcaa
      241 gaacgtttag aaagagaatt tgatcttgat ttaattgcaa cggcaccatc agttatttat
      301 cattgctatt tgacagatcg tagtgaagta atcgttgata atccagcgat gatgcctgag
      361 ccgcaagtaa ttgatcatat tgaagaacct tatgttaaag cttctattat gactccaaac
      421 gaatacgtag gggttatcat ggaattatgt caatctaaac gtggagaata tcaggatatt
      481 gaatatattg atgatactcg acgtaatgta atttatgaaa ttcctttatc tgaaattgta
      541 tatgattttt ttgacaaatt aaaatcaggt actaaaggct atgcatctct tgattatgaa
      601 ttgattggct atcgtactag caaacttcaa aaaatggata ttttattgaa cggagaagtc
      661 gttgatgcac tttcaacaat tgtgcataag gattttgcat atggacgagg aaaaattatc
      721 tgtgaaaaat taaaagaaat tattccaaaa caaatgtttg aagtgccaat tcaagcagca
      781 ctacaaggga aaataattgc ccgtactaca attaaggcga tgcgtaaaaa tgtattggca
      841 aaatggtaag cctgcgcctg ataatccatg gtttaaccag gatgctacgc atccctttag
      901 tgtatttttt gattttaatc acnnnnnnnn nnggcggcga tatttctcgt aaaaagaaat
      961 tactagagaa acaaaaagaa ggtaaaaaac gaatgaaggc agttggaaat gttgaaattc
     1021 cacaagaggc attcatggcg atattatctg ttgacgatga gtaggataaa ttatctatcc
     1081 tacttatttt tttgttgtaa atagtcttga tagccattac aatataaata tatacgaata
     1141 aaggagaatg aaaatgacaa tcaaattacc agacaatttt ttctttgggg cggctttatc
     1201 tggtccgcaa actgaaggtg cttataacgt tggtggtcgc ctaagatcat actgggacat
     1261 gtggtctgac caggaaatca atgcttttca taacaatgtt ggctcatatg tcggaaatga
     1321 tatgtatcac cgttatgaag aagacataaa actgttcaaa tcaatgaact ttaaatcttt
     1381 tagaacatca atgcagtgga ctagactgct tgatcaggat ggaaatatta atccagaagg
     1441 agctgcatgg tatcataaac tgatcgattg tgccaatgaa aacggaatcg atatctttat
     1501 gaacatgtat catttcgata tgcctgaata cttatataaa cgtggtggtt gggctaatcg
     1561 tgaagttgtt gaagcttatg caaattttgt taaaaaggca atggaggaat ttggtggcaa
     1621 agttaaatat tggtttacat tcaatgagcc aatcgttgaa ccagaacagc aatatttgca
     1681 tggggtttgg tttccctatg aaaagaacat taaacgttca ttggatgtcc aatataatat
     1741 cacattagca cactgtctag cagtaatgaa ctttagagaa ctgcaggcta gagggaaaat
     1801 ggttgaagga gcgaagatag ggatgatcaa ctgctttgca ccaccatata caaaagaaga
     1861 tccaagtcca gaggatctgg aagcagtaag aatgacag
//
LOCUS       DAOMTA010000652         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3057_length_1897_cov_2.023344, whole genome shotgun sequence.
ACCESSION   DAOMTA010000652 DAOMTA010000000
VERSION     DAOMTA010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3057_length_1897_cov_2.023344"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..908)
                     /locus_tag="PLK68_14070"
     CDS             complement(27..908)
                     /locus_tag="PLK68_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM91574.1"
                     /translation="MELRVLKYFLAVAREENITKAAEFLHITQPTLSRQLMQLEEELN
                     AQLFIRGKNRIVLTDEGMLLRRRAEEIVDLANKTEKEFLEQDNLVTGEIFIGGGETNA
                     MHILARIIKEFKEEYPQIKYQFYSGNADDIKERLDKGLIDIGLLTEPVDIEKYEFVRL
                     EQKEVWGILAPKDSKLAAKEYATPQDLLKLPLLSTRRTIVQNEIANWFGQDYEQLDII
                     ATYNLIYNAAIMVEEGIGYAICLEKLVNINDETKIRFIPFYPPLLTGTVIVWKKHQIF
                     SPATARFIEKIKHSLKA"
     gene            complement(1434..1856)
                     /locus_tag="PLK68_14075"
     CDS             complement(1434..1856)
                     /locus_tag="PLK68_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91575.1"
                     /translation="MENNQKKEVNEALHAIEETLGHLSRAQDYLSSAGNWGLFDMIGG
                     GFITTMIKHGKMNEAERAMAAARNSIRNLKKELSDVDQLVDVDLNISDFLSFADYFFD
                     GIIADWMVQSKIKDARFQVDKAIRELNRIKNTLLTLAV"
BASE COUNT          613 a          366 c          199 g          719 t
ORIGIN      
        1 gtctaatacc tataatctat aatcaatcat gcttttagag aatgctttat tttttcaata
       61 aatcgcgctg tagcaggtga aaaaatttga tgctttttcc atacgattac tgttccagtt
      121 aacaatggag gataaaatgg aataaagcga atttttgttt catcattaat attgactaac
      181 ttttctaaac aaatcgcata cccaatcccc tcttctacca taattgcagc attataaata
      241 agattgtatg tcgcaataat atctaattgc tcataatctt gcccaaacca attagctatt
      301 tcattttgaa cgattgttct tcgagtactt aataatggca attttaataa atcttgtggt
      361 gttgcatatt ccttagctgc aagtttacta tctttaggtg ctaaaatacc ccaaacttcc
      421 ttttgctcta agcgtacaaa ctcatacttt tcaatatcaa ctggttcagt cagcaaacca
      481 atatcaatca atcctttatc taaacgctct ttaatatcat cagcattccc actataaaat
      541 tgatatttga tttgaggata ttcttcttta aattctttga ttattctagc aagaatatgc
      601 atagcatttg tttccccacc accaataaaa atttctcctg taactagatt atcttgttct
      661 aaaaattctt tttccgtttt attagctaga tctacaattt cctcagctct tcttcgaagc
      721 agcattcctt catctgttaa aacaattcga ttttttcctc ggataaataa ttgcgcgttt
      781 aactcctctt caagctgcat cagttgtctt gataaggttg gttgagttat atgtaaaaac
      841 tcagcggctt ttgtgatatt ttcttcacgt gctaccgcta aaaaatactt aagtactctt
      901 aattccatgc ttacacctca ctttatttta ttatataaaa tttatgacaa aattcctaat
      961 ccaatctaaa gcaatttaaa ataagtccat cgtactataa aacagtctat cctattatag
     1021 actgttttca ttatataaat tctacttctc tttcatttaa tcttatgaca ttcatactta
     1081 cataagtaaa tacattaata attccactga atatatgcca aaaactagtg aattcttttg
     1141 atttgtgttg tctagttata taaaatattt cagtattttt ctaagattcc ttcccaaaat
     1201 acaaatattc aattttattt aaatgaattt tcatcaaata cacttgcttc agacagtacg
     1261 aacatattca taaatggttg aaaaccatat attaacatta atccattttc ttttaaacaa
     1321 cttgttacct ttaaaaattg tacttctaaa tcttcaaact aagtagttac ttcaatcatt
     1381 tgattgatta tgttatccat aatatcaaat cataccttca ttttacaata tttctagact
     1441 gcaagggtca gtaacgtatt tttaatacga tttaattctc gaatagcttt atcaacttga
     1501 aatcgagcat ctttgatctt tgattgaacc atccaatcag caatgattcc atcaaagaaa
     1561 taatctgcaa aacttaaaaa atcactaata tttaaatcaa catcaaccag ctgatctaca
     1621 tcacttaatt ctttttttag atttcgtatc gaattacgtg ctgctgccat tgctctttca
     1681 gcttcattca ttttaccatg tttgatcatc gttgtaataa aaccaccacc aatcatatca
     1741 aaaagtcccc agtttcctgc actacttaaa taatcctgag cccttgataa atgacctaaa
     1801 gtttcttcaa ttgcatgaag tgcttcatta acttcttttt tctgattatt ttccataaac
     1861 tttccccttt gttatcaaat ttcaagaata ttttacc
//
LOCUS       DAOMTA010000653         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3058_length_1897_cov_1.907166, whole genome shotgun sequence.
ACCESSION   DAOMTA010000653 DAOMTA010000000
VERSION     DAOMTA010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3058_length_1897_cov_1.907166"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLK68_14080"
     CDS             complement(<1..259)
                     /locus_tag="PLK68_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM91576.1"
                     /translation="MICMIRMDDRLLHGQVAYAWKADLGYNAVVIASDEASKDDVKKA
                     ALKMCCPSDVRLAIRTVEDALKLLKNPKLESLRVLVVMDNTK"
     gene            complement(278..700)
                     /locus_tag="PLK68_14085"
     CDS             complement(278..700)
                     /locus_tag="PLK68_14085"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM91577.1"
                     /translation="MNKLLICSHGSLSKGLFSSLNIIMGDVENVDYLTFYENGEGAAQ
                     LEKIENYFKENKEYQLIVLTDVFGGSVNQEVIKKAKDYDNIRIVSGVNFPLLLELAIR
                     LQTEITDEELKNLIEQSKNQMLMVETKLETEFKDDFDF"
     gene            complement(716..1723)
                     /locus_tag="PLK68_14090"
     CDS             complement(716..1723)
                     /locus_tag="PLK68_14090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRM91578.1"
                     /translation="MYNYDVENFKNEGKAVYDLRSEIERIVDEVCKNGVSNVILTGIG
                     GTIFELMSIKKIFDDYSKLDCVIMNGADMLIENPDWVDEHSVVITGSKSGDTPETIAA
                     CKYCRERGAKVISLVGTNDCPLANCSDYVVVSDISGMTHTYLKFYLIALRFIYNNKEF
                     DAYEKFADECRDLYESVVKIQEKFEPIAASIADKYSNEPYQIWVGSGIVWGEINLFTM
                     CILEEMQWMRTRAVTSSDFFHGTLELVEKDVPVYLIKGIGKYRPQDERVERFLEKTTD
                     KLVVIDLEDYKLDCFSKEFEEMLTPITFNALTRGRLAYHFERVTGHDLNIRRYYRQFE
                     Y"
BASE COUNT          613 a          366 c          193 g          725 t
ORIGIN      
        1 ctttagtatt atccataact actaaaactc ttaatgattc aagttttgga ttttttaaca
       61 attttaatgc gtcctctact gttctaattg ccaatctaac atctgaagga caacacatct
      121 ttaatgcagc cttttttaca tcatcttttg aagcctcatc tgaagcaata acaactgcat
      181 tatatcctaa gtctgctttc caagcatacg ctacctgtcc atgcaataat ctatcatcca
      241 tgcgaatcat acaaatcatt gaaattcacc tcctatttta gaaatcaaaa tcatctttaa
      301 attctgtttc taattttgtt tcaaccatta gcatttgatt tttggattgt tctataagat
      361 tttttaattc ttcatcagta atctctgttt gcaaacgaat agctaattct aataaaagag
      421 ggaagtttac tccagataca atacgaatat tatcgtaatc cttcgctttt tttattactt
      481 cttgatttac acttccccca aatacatctg taagcactat tagttgatat tctttatttt
      541 ctttaaagta attttcaatt ttttctaact gtgctgctcc ttctccattt tcgtaaaatg
      601 ttaaataatc aacattttct acatctccca tgattatatt caaactacta aataaccctt
      661 ttgataatga accatgagaa catattaaaa gtttgttcat ttgaataatc tccttttaat
      721 attcgaattg tctgtaataa cgacggatat ttaaatcatg tccagtaaca cgttcaaagt
      781 gatatgctaa acgtcctcgt gttagtgcat taaatgtaat cggagttaac atttcttcaa
      841 attctttaga gaaacaatct aatttataat cttctaagtc aataacgact aatttatcag
      901 ttgttttttc caagaatctt tctactcttt catcttgtgg tcgatatttt ccaatacctt
      961 ttattaaata cacaggaaca tctttttcta ctaattctag cgtaccatga aagaaatcag
     1021 aacttgtaac tgctcgtgtt cgcatccatt gcatttcttc aagaatacac atagtaaata
     1081 aattaatttc tccccataca attccagatc ctacccaaat ttgatatggt tcatttgaat
     1141 atttatcagc aattgatgct gcaattggtt caaatttttc ttgaatcttt acaacagatt
     1201 catataaatc tctacattca tcggcaaact tttcataagc atcaaattct ttattgttgt
     1261 aaataaatct aagagcaatt aaatagaatt tcaaataagt atgtgtcatt cccgaaatat
     1321 cagatacaac aacataatca gaacaattcg ctaatgggca atcatttgtt cctactaaag
     1381 aaattacttt tgctcctctt tctctacaat atttacatgc agcaatcgtt tctggtgtat
     1441 cccctgattt gcttcctgta atcacaacac tgtgttcatc aacccaatca ggattttcaa
     1501 ttaacatatc agccccattc ataataacac aatctagttt tgaataatca tcaaaaattt
     1561 tcttaattga cattaattca aaaatagttc caccaatacc agttaaaatt acattagata
     1621 ctccattctt gcaaacttca tccacaattc tttcgatctc acttcttaga tcatatactg
     1681 ctttaccctc gtttttaaaa ttttctacat catagttata catatctttt ttcctccttt
     1741 gtataccaga tgtctatata ttaaaccaaa aatttaaaaa atgcaaccac ttttttaatt
     1801 tttttaccaa tttttattat caaactattt aaaaccctta catttgaata ttcaagataa
     1861 attggtttgt gttgatagaa attggttttt cgtgtat
//
LOCUS       DAOMTA010000654         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3077_length_1886_cov_2.048607, whole genome shotgun sequence.
ACCESSION   DAOMTA010000654 DAOMTA010000000
VERSION     DAOMTA010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3077_length_1886_cov_2.048607"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLK68_14095"
     CDS             complement(<1..181)
                     /locus_tag="PLK68_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91579.1"
                     /translation="MRYIHTLEEKKFKEEKEKENELLRKFGVDEKIIQELYDYDREMF
                     NKNRSFSQHEVYIETL"
     gene            complement(639..926)
                     /locus_tag="PLK68_14100"
     CDS             complement(639..926)
                     /locus_tag="PLK68_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91580.1"
                     /translation="MKISINEKGVLFVDDKEKKITDLNAEFLENLVNKSLTDEVVYDI
                     NSDSPIGEFFKKIQEETAVESDLRTKIIELKEAKKQKLEEINELIPNYEKV"
     gene            complement(916..>1886)
                     /locus_tag="PLK68_14105"
     CDS             complement(916..>1886)
                     /locus_tag="PLK68_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91581.1"
                     /translation="LNKKMKSLDSSICELSEKMSKQEIIYNAVIEYNSKIGTQYQTIV
                     ERRQNLMTNFNKIKDLVVKNITLINSLKIPVLSEDIIKNSIDFKNQDIAQLTFSKINL
                     SINKDDLKDAFPTNIGNKPKLKASLFSVQNLNLGDETCLKEFLQTFIDNNYDQQIQLS
                     FNDASFVDYKIELKTLEGNFENIDNITAGNLSKIYINKMFEDKIIHAGSNTIILYDQP
                     DANMEKEFILSELVTKISKIRDTNQIFITTHEPLLVVNSDSNNIIAATNEKTAIKKND
                     IEYTNKSFVGVNSRKELVQEVAKLIDGSPEAVKRRSIIYGGVLNED"
BASE COUNT          596 a          320 c          198 g          772 t
ORIGIN      
        1 gtagtgtttc aatgtaaacc tcatgttgac taaatgacct atttttatta aacatctctc
       61 gatcataatc atatagttct tgaataatct tttcatcaac accgaacttt cttaaaagtt
      121 cattttcctt ttctttttct tctttaaatt tcttttcttc taatgtatgt atatacctca
      181 tttctgcctc ctaaatttta tgtaatttag aaggctatag gcggtcctcg tatcacgcta
      241 atcatcccct tcaataacta gccatcgaaa acccctaaaa agtttacaaa taattaaaaa
      301 aagtgacaaa atcgtgaaat ttcttcacga tacgtcactc ttaaacaaat attcaattgt
      361 atatatacat taaaagaaaa cttcattact gctaacccct tacacaaggg ttattgctaa
      421 tatgaatgtt agtaacacct tgccacaccg ttaaatgaaa tattattata atatatcatt
      481 tactgttatg acagttggta agcctaaagc atactttttt gacttaagat gcattatctg
      541 ttgatttaca ataattaaag caaattttgt attcaaacaa atctataccc tcacattgcg
      601 aatatattat tattaaatta tataagaaaa ttaagttgtt aaactttctc ataatttggt
      661 atcaattcat ttatttcttc tagcttttgc ttttttgcct ctttcagctc tattattttt
      721 gttcttaaat ctgattcaac agctgtttct tcttgtattt tcttaaaaaa ttcaccaatc
      781 ggtgaatctg aatttatgtc atacacgact tcatcggtta atgacttatt cacaagattc
      841 tctaaaaatt cagcgtttaa atcagtgatt ttcttttctt tatcatcaac aaatagtaca
      901 cctttttcat taatactaat cttcatttag tacgcctcca taaataatac ttcttctctt
      961 tactgcttca ggactaccgt caattaattt tgcaacttct tgaaccaatt cctttctgct
     1021 gtttacacca acaaatgatt tattggtata ttcaatatca tttttcttaa ttgctgtctt
     1081 ctcattagta gctgcaatta tattgttcga atcagaattt actaccaaca agggttcatg
     1141 agtagtaata aatatttgat tagtatctct aatttttgat attttagtaa ccaactctga
     1201 taaaataaat tccttttcca tattcgcatc aggttgatca tataagatta tagtattact
     1261 tcccgcatgg attatcttat cttcgaacat tttatttata tagattttac ttaaatttcc
     1321 agcagttata ttatcaatat tctcgaaatt cccctcaagt gtttttaatt caatcttata
     1381 atctacaaaa gaggcatcat taaatgataa ttgaatctgc tgatcataat tattatcaat
     1441 aaatgtctgt aaaaattctt ttaaacaagt ctcgtcacct aaattcaaat tttgaacact
     1501 aaataatgat gcttttagtt ttggcttatt tcctatattg gttgggaatg catcttttaa
     1561 atcatcttta tttatactta agtttatttt actaaaagta agttgagcta tatcttgatt
     1621 tttaaagtca atagaatttt ttataatgtc ttcacttaaa actggtattt ttaatgaatt
     1681 tatcaacgtt atatttttga caactaagtc tttgatttta ttgaaattag tcattaaatt
     1741 ctgtcgtcgt tccacaatcg tttgatactg tgttccaatt tttgaattat actcgatgac
     1801 tgcattatat attatttctt gcttactcat tttttcactc aattcacaaa tagatgaatc
     1861 aagcgatttc atcttcttat ttaatg
//
LOCUS       DAOMTA010000655         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3080_length_1885_cov_1.645355, whole genome shotgun sequence.
ACCESSION   DAOMTA010000655 DAOMTA010000000
VERSION     DAOMTA010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3080_length_1885_cov_1.645355"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLK68_14110"
     CDS             complement(<1..816)
                     /locus_tag="PLK68_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM91582.1"
                     /translation="MEFLKALFKIISSKKITHTLLNVMIVLVILLLIIATGDLWYGVL
                     SAIWLVSKPFIVGFTIAFVLNPLINYIEKYVTKRSIAVTMVYLAAFAILTLLISLAVP
                     MIYESISEMFPAFYSGLGEIGVFVKENFNYDISSLMRHIQNIVNAFFKDSVVLDTTID
                     VLNQVMINVTNFLIYIILAIYMSSNFKNIRSHIRNITYRIDKTLPIYLREIDSSLVQY
                     VKAFFIGAIAQAITTMLMYLAIGHPNWLILGFVSGASSIIPCVGPIVANCLGLI"
     gene            1146..>1885
                     /gene="sufC"
                     /locus_tag="PLK68_14115"
     CDS             1146..>1885
                     /gene="sufC"
                     /locus_tag="PLK68_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRM91583.1"
                     /translation="MSTLKIENLHVSIGEKEILKGIDLVVNTGETHAIMGPNGNGKST
                     LLSVIMGHPKYIVTQGSIYIDDQNVLEMSVDERSRAGIFLGMQYPQEIPGVTTSDFLR
                     AALNAHQEKPVSLFKFVKALDKNIADLKMDENLAHRYLNEGFSGGEKKRNEILQMKLL
                     QPKFALLDEIDSGLDVDALKIVSQAINAMKSDDFGCIMVSHYERLFELVPPSHVHVLV
                     NGKIILSGGIEVVEKIDQEGYDWVKELGV"
BASE COUNT          725 a          240 c          313 g          607 t
ORIGIN      
        1 aattaatcct aaacaattcg caacgattgg tccaacacat ggaataatgg agctggctcc
       61 tgaaacaaac ccaagaatta accagttagg atgaccaatt gctagataca tcagcattgt
      121 tgtaattgct tgagcgattg ctccaataaa gaaagcttta acatattgta ctaaagaaga
      181 atcaatttca cgtaaataga ttggcaatgt tttatcaata cgatatgtaa tattacgaat
      241 atgactacgg atatttttaa aattactaga catataaatt gctagaatga tgtagattaa
      301 aaaattagtg acattgatca taacttgatt taaaacatct atagttgtat ctaaaacaac
      361 tgaatcttta aagaaagcat tgacaatatt ctgaatatgg cgcattaaag aagaaatatc
      421 ataattgaaa ttttccttaa caaacacacc aatttcacca agtcctgagt aaaaagccgg
      481 gaacatttca gagatacttt cataaatcat tggtacagct aaagaaatta ataaagttaa
      541 aatagcaaaa gcagctaagt agaccattgt tacggcaatg cttcgtttag taacatactt
      601 ttcaatataa tttatcagtg gatttaaaac aaaggcaatt gtaaatccga caataaatgg
      661 tttagaaacc agccagatcg ctgataacac cccataccat aaatcaccag tggcaataat
      721 taatagtaat attactaaaa cgatcatcac atttaataaa gtatgggtga ttttttttga
      781 tgaaataatt ttaaataatg cttttagaaa ttccattttt acacccccat tacatataaa
      841 tataccacaa tttactaata atataaattg aattgaattt ctattttaga aaaatcaaaa
      901 atagttaaaa tgtataaagt tttataattt tcatccaaaa aaagcaattg gatgttatga
      961 ttagaatatt ataatagagc aaatctcttg ataattatcg ataatttggt cacaatgtgg
     1021 ttataatata aattgtgcta gattttgatt ggaaaatagt aaaaattttt gctataattt
     1081 atcattttat ttgttttttg ataacttatc tagtagaatt agtaaggata gaaaggggct
     1141 taattatgtc aactttaaaa atagaaaatt tgcatgtcag tattggagaa aaagaaatct
     1201 taaaaggtat cgatcttgtt gttaatactg gtgaaaccca tgcgattatg gggccaaatg
     1261 gtaatggaaa atcaacatta ttatcagtta tcatggggca tcctaaatat atagttactc
     1321 aaggtagtat ctacatagat gatcaaaatg ttttagaaat gagtgttgat gaaagaagta
     1381 gggcaggaat ctttttagga atgcagtatc ctcaagaaat tccgggtgtt acaacttcag
     1441 attttttaag agctgcatta aatgcacatc aagaaaaacc agtatcttta tttaaatttg
     1501 taaaagcact ggataaaaat attgctgatt tgaaaatgga tgagaattta gcacatcgtt
     1561 atctaaatga aggcttctct ggtggagaaa agaaacgtaa tgagattcta caaatgaaat
     1621 tattacaacc taagtttgct ctattagatg aaattgattc aggtttggac gttgatgcgt
     1681 taaaaatagt atcacaagca attaacgcta tgaaatcaga tgattttgga tgtattatgg
     1741 tttctcacta tgaacggttg tttgaattgg tgccaccaag tcacgttcat gtattagtta
     1801 atggtaaaat tattttaagt ggtggaattg aagtagtaga aaaaattgat caagaaggct
     1861 atgactgggt aaaagaatta ggagt
//
LOCUS       DAOMTA010000656         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3084_length_1884_cov_1.785128, whole genome shotgun sequence.
ACCESSION   DAOMTA010000656 DAOMTA010000000
VERSION     DAOMTA010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_1884_cov_1.785128"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLK68_14120"
     CDS             <1..465
                     /locus_tag="PLK68_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRM91584.1"
                     /translation="TFYHGVVATVTLIVVDRLVSMITMKSKKLRDIFEGRPTYIIFKG
                     KLDQEIMKKQRYTVDDLSHHLRVNDIDSISKVEFALLETNGSLSIIPKDQCVVELPDA
                     LICDGIIDEDNLKLLNRDINWLKKELKKQGVDKIEDVFYCVPEKGHLLVIKK"
     gene            complement(480..1274)
                     /locus_tag="PLK68_14125"
     CDS             complement(480..1274)
                     /locus_tag="PLK68_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91585.1"
                     /translation="MLKLEHVSKTFNLGTVNEKKALQDLNLTVNDGDFITIIGGNGAG
                     KSTTLNMIAGVYPIDCGYITIDNQDISLADEYKRAKFIGRVFQDPMMGTAANMEIQEN
                     LAMAFRRGKRRGLSWGISKEEKKQYHEALKRLDLGLETRMSSKVGLLSGGQRQALTLL
                     MATLQKPKILLLDEHTAALDPRTAKKVLDLTEEIVTEQKLTALMVTHNMKDAINIGNR
                     LIMMDKGRIIYDVNGEEKAKLTVDDLLKKFEEASGSEFDNDRMLLG"
     gene            complement(1267..>1884)
                     /locus_tag="PLK68_14130"
     CDS             complement(1267..>1884)
                     /locus_tag="PLK68_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91586.1"
                     /translation="INLRIMGKSNTPLLQSDTIFKGLSNTFNLSQAWITLIIGIICAI
                     IVILICYWFFGTEIGSAIRATGNNEHMVRALGANTNTTKLLGLMISNGLIAMSGALVT
                     QSQGYADIKMGIGAIVIGLASIVIGEVILGRKPGFMFTLTAIIVGSILYRIIVAVVLQ
                     LGLSTDDLKLLTALLVGIALTVPVMVSKRKQIATYKKLTKEKENA"
BASE COUNT          620 a          327 c          291 g          646 t
ORIGIN      
        1 actttttatc atggtgtggt tgcaactgtt acattgattg ttgtagatcg acttgtttca
       61 atgataacaa tgaaatcaaa aaaacttcgc gatatttttg aaggacgacc tacatacatt
      121 atttttaaag gaaaattgga tcaagaaata atgaaaaagc aacgatatac tgttgatgat
      181 ttatcacatc atttacgagt taatgatatt gatagtattt ctaaagttga atttgcttta
      241 ttagaaacaa atggttcact ttcaattatt cctaaagatc agtgtgtagt agaattaccg
      301 gatgctttaa tctgtgatgg gataattgat gaagataatt taaaattgct caaccgggat
      361 ataaattggt taaaaaaaga gttgaaaaaa caaggtgtag ataaaataga agatgttttt
      421 tattgtgtgc cagaaaaggg ccacttatta gtaataaaaa aatagagctg gaatcagctc
      481 tatcctaata gcatgcgatc gttgtcaaat tcactaccgc ttgcttcttc gaatttttta
      541 agtaaatcat caactgttaa tttggctttt tcttcaccgt tgacatcata aataatacgg
      601 cccttgtcca tcataattaa gcgattaccg atattaatag catctttcat attatgtgtt
      661 accattaaag cagttagctt ttgttcagtg acgatttcct ctgttaaatc aagaactttt
      721 ttagcggttc gaggatctaa tgctgcagta tgctcatcaa gtaaaagaat tttgggtttt
      781 tgaagtgttg ccatcagtaa tgttaaagct tgacgttgtc ctcctgataa caaaccaact
      841 ttacttgaca tccttgtttc taaacctaag tctaaacgtt ttagtgcttc atgatattgt
      901 tttttttctt ctttgctgat tccccagctt aaaccacgac gtttaccacg acgaaaagcc
      961 atagccagat tttcttgaat ttccatatta gctgctgttc ccatcatagg atcttggaaa
     1021 acacgtccaa taaatttggc acgtttatat tcatcggcga gcgagatgtc ttgattgtca
     1081 atagtaatat atccacagtc aattggataa accccagcaa tcatatttaa agtcgtactt
     1141 ttaccagccc cgtttccacc gatgattgta ataaaatctc catcattaac agtaaggttc
     1201 aaatcttgaa gagccttttt ttcattaaca gtaccaaggt taaaagtttt acttacatgt
     1261 tctaatttaa gcattctctt tctcctttgt taattttttg tatgtagcaa tttgttttct
     1321 tttacttacc ataacaggta cagttaatgc tatacctact aataaagctg tcaacagttt
     1381 taaatcatca gtacttagac ccaattgtaa aacaactgca acaataatac gatacaaaat
     1441 tgaaccaaca ataatagcag ttaaagtaaa catgaaacca ggctttcttc ctaggattac
     1501 ttctccaata acgattgaag ctaaaccaat aacaattgct ccaattccca tcttaatgtc
     1561 ggcataacct tgagattggg ttactaatgc accagacatt gctattaaac cattagaaat
     1621 cattaaacct aataatttag ttgtatttgt attagcacca agtgctcgaa ccatatgttc
     1681 attatttcct gtagcacgga tagcactacc aatttcggtt ccaaagaacc agtaacatat
     1741 taaaataacg attatcgcac aaattattcc gatgattaaa gtaatccatg cttgtgatag
     1801 attgaaagta tttgataaac ctttgaagat agtatcactt tgaagtaaag gtgtattact
     1861 ttttcccatg attcgtaaat taat
//
LOCUS       DAOMTA010000657         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3090_length_1882_cov_1.998905, whole genome shotgun sequence.
ACCESSION   DAOMTA010000657 DAOMTA010000000
VERSION     DAOMTA010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_1882_cov_1.998905"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1072
                     /locus_tag="PLK68_14135"
     CDS             <1..>1072
                     /locus_tag="PLK68_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM91587.1"
                     /translation="VMDVVYNHMYDVNASAFERIVPGYYFRKNPDGSLSNGSWCGNDL
                     DSGEHMVRKYIIDMSRRWQSFYGVDGYRFDLMGIIDIETINRVYQICSAYDASFMVYG
                     EGWNMPTNLPDEQKAMQDNHAKMPKISFFNDEYREVIKGGSSDNVLVNKGFITGNLYE
                     TEKARNVITATNRYTSPDQSINYVECHDNATVFDKLYISNVEEGLDGIITRQKNLTIM
                     VLLSQGIPFIHAGQEFYRTKGGIGNTYNSPDNINCINWDFRDIYKDDINEIRKIIQLR
                     KDNKCFRYATRKEIEANVMVENIDFKMLKYTLKQDEGEYREFVIYINPSSFTFDYEET
                     AYQHLYGEITDQQIGARTIVVLA"
     assembly_gap    1073..1082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1097..1651
                     /gene="pth"
                     /locus_tag="PLK68_14140"
     CDS             1097..1651
                     /gene="pth"
                     /locus_tag="PLK68_14140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRM91588.1"
                     /translation="MKLIVGLGNPGKEYEGTRHNCGFMVVDELANKLNTEINQNKFKG
                     LYTKVKYHGEDVILLKPQTYMNLSGESVIAAMNFFKLDKEDIIVIYDDLDMPVGKLRL
                     RKTGSAGGHNGIKNIIAHLSSQDFKRIRVGIDRHKYMKVVDYVLSRFAKEETEAINQG
                     IDKASDAVLDYLDHDFDYIMNRYN"
     gene            1660..>1882
                     /locus_tag="PLK68_14145"
     CDS             1660..>1882
                     /locus_tag="PLK68_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91589.1"
                     /translation="MKKIDEILKQNPAFKTLLKGEGNIIVNDLNDEALLVTSAFLTLQ
                     KDIIVIKPNQYEANLLYQQISLINEKDSLF"
BASE COUNT          657 a          204 c          394 g          617 t
ORIGIN      
        1 tagtgatgga tgttgtctat aaccatatgt atgatgttaa tgcgagtgct tttgaacgaa
       61 tcgtacctgg ctactatttt agaaaaaatc cagatgggag tttatcaaat ggttcatggt
      121 gtggtaatga tttggatagt ggtgaacata tggtgcgtaa gtatattata gatatgtcga
      181 gacgctggca aagtttttat ggtgttgacg ggtatcgttt tgatttaatg ggaattattg
      241 atattgaaac gattaatcgt gtctatcaaa tttgttctgc ttatgatgct tcatttatgg
      301 tttatgggga gggttggaat atgccgacta atttaccaga tgagcaaaag gcgatgcagg
      361 ataatcatgc aaaaatgccg aaaattagtt tttttaatga tgaatatcgt gaggttatta
      421 aaggtggaag cagtgacaat gttttagtta ataaaggttt tattacggga aatctttacg
      481 aaacagaaaa ggcaagaaat gtaattacag cgactaatcg ttatacaagt ccggaccaat
      541 caattaatta tgttgaatgt catgataatg cgacggtttt tgataaattg tatatttcta
      601 atgttgaaga aggtttagat gggataataa ctcgacaaaa gaatcttacg atcatggtgt
      661 tattgtcgca gggaattccc tttatccatg cgggacaaga attttatcga actaaaggcg
      721 ggattgggaa tacttataat tcacctgata atattaactg tattaattgg gattttcgag
      781 atatttataa agatgatatt aatgagataa gaaaaattat tcaattacgt aaagataata
      841 agtgttttcg ctatgcaact agaaaagaga ttgaagcaaa tgttatggtt gaaaatattg
      901 actttaaaat gttaaaatat actctaaaac aagatgaagg tgaatatcgt gaatttgtta
      961 tttacattaa cccatcaagt tttacatttg attatgaaga aactgcttat cagcaccttt
     1021 atggtgaaat tactgatcag caaataggag ctagaacgat tgttgttcta gcnnnnnnnn
     1081 nnataggaga attaaattga aattaattgt aggattggga aaccctggga aagagtatga
     1141 aggaactcgt cacaactgtg ggtttatggt agtagatgaa ttagctaata aattaaatac
     1201 agaaataaat cagaataaat tcaaaggttt atatacaaag gtaaaatatc atggagaaga
     1261 tgttatttta ttaaagccgc aaacatatat gaatctttct ggtgagtcag taattgctgc
     1321 aatgaatttt tttaaactgg ataaagaaga tattattgtt atctatgatg atctggatat
     1381 gccggttggt aaattaagat tacgtaaaac tggcagcgct ggggggcata atgggataaa
     1441 aaacattatc gctcatttat ctagtcaaga ttttaaacga attcgtgtgg gaattgatcg
     1501 tcacaagtat atgaaagtag ttgattatgt tttatcaagg tttgcaaaag aggagactga
     1561 agcaatcaat caaggaattg ataaggctag tgatgctgtt ttggattatc ttgatcatga
     1621 ttttgattac attatgaatc gttataatta gaggtttata tgaagaagat agatgaaata
     1681 ttgaaacaaa accctgcctt taaaacattg ttaaagggag aggggaatat cattgttaat
     1741 gatcttaacg atgaggcact attagtaact agtgcctttt tgacattaca aaaagatatt
     1801 attgtaatta aaccaaatca gtatgaggct aacttgttgt atcaacagat ttcattgatt
     1861 aatgaaaaag acagcttatt tt
//
LOCUS       DAOMTA010000658         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3096_length_1880_cov_1.951233, whole genome shotgun sequence.
ACCESSION   DAOMTA010000658 DAOMTA010000000
VERSION     DAOMTA010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_1880_cov_1.951233"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLK68_14150"
     CDS             <1..226
                     /locus_tag="PLK68_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2000 family protein"
                     /protein_id="HRM91590.1"
                     /translation="DEFLIRELLKQANYYPNEVLVIDFCDLAQSCRDYSEYIDKMKLA
                     SEASLKYFGICLYGTRTRINKLTGNLSLLK"
     gene            285..1007
                     /locus_tag="PLK68_14155"
     CDS             285..1007
                     /locus_tag="PLK68_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HRM91591.1"
                     /translation="MENIELDVHTHTIASGHAYSSLSEMVAGAKEKDIKLLGIVEHDQ
                     GIPGTCAPIYFKNLDVIPREIEGIKLLLGIEINILDHQGRLSITEDLYDYVDYCMAGI
                     HLHCYEAGTIEENTRAVIETIKNPHISVIVHPDDGKCPLDYEKVVLAAKKYHTLLEVN
                     NNALRSPSRLNSRENTLKMLQLCKQHRVKIILGSDAHIHFDIKNYDQIEELLKEVEFP
                     KELIVNYHIEDFLEYIKKQVRY"
     gene            complement(1091..1342)
                     /locus_tag="PLK68_14160"
     CDS             complement(1091..1342)
                     /locus_tag="PLK68_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91592.1"
                     /translation="MNEMYVISVLMLYTLIILINTLLNTYRLLTTTEMVEEKEFIYPK
                     SSIYHRVHTYVAPMILRINMKKDYVLNKKRRPPIILMAH"
     assembly_gap    1642..1658
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          675 a          244 c          324 g          620 t
ORIGIN      
        1 tgatgagttt ttaatcagag aactactaaa acaggctaat tattatccta atgaggtatt
       61 agttattgat ttttgtgacc ttgctcaaag ttgtcgtgat tattcagaat atattgataa
      121 aatgaagctg gctagtgaag cttcgcttaa atattttggg atttgtctat atggtacaag
      181 gacacgaatt aataaactaa caggaaattt atcattacta aaatagaaaa aaagatgttt
      241 ttagccacca aataggctat gatatagcca ttggagggat aaaaatggag aatattgaat
      301 tagatgtaca tacacataca atagcaagcg ggcatgctta ttcatcactt agtgaaatgg
      361 tagctggggc gaaagaaaag gatataaaat tattaggaat agtagagcat gaccagggaa
      421 ttccaggaac atgtgctcca atttacttca aaaatctaga tgtaattcct cgagagatag
      481 agggaattaa attattgtta gggattgaaa ttaatatttt ggatcatcaa ggaagactta
      541 gtattacaga agatttatat gattatgttg attactgcat ggcaggaatt catttacatt
      601 gttatgaggc gggaacaatt gaagaaaata cacgggcagt gatcgaaaca attaaaaatc
      661 ctcatattag tgtaatcgtt cacccagatg atggtaagtg cccacttgat tatgagaaag
      721 tagttttagc agctaaaaag tatcatactt tgttggaagt gaataataat gctttacgta
      781 gtccatctcg tctaaattca cgtgaaaata cacttaagat gctacaatta tgcaaacaac
      841 atcgagttaa aattatttta ggtagtgatg cacatattca ttttgatata aaaaattatg
      901 atcaaatcga ggaactctta aaagaagtag agtttccaaa ggaattaatt gttaattatc
      961 atattgaaga ttttttagaa tatataaaaa aacaagtgag atactaattc tcacttgttt
     1021 tcatatttgt taaatttata tctgaaatta attaaactct attaactatt ttttcatatt
     1081 agactcaacc ttagtgagcc attaatataa ttgggggtct acgtttctta tttagtacgt
     1141 aatccttttt catattaatt cttaggatca ttggagctac ataagtatga accctatgat
     1201 agattgaaga tttaggataa ataaattctt tttcttcaac catctcagtt gttgttaaca
     1261 gacgatatgt atttaacaat gtattgatta aaataatcaa tgtataaagc attaacactg
     1321 aaattacata catctcattc atattatcaa ctcctttcta ctaatccttt cctcaccatt
     1381 atattagcat attttatcca aaaatgaaag ggctttcaat gaaaaaatga ttttttattt
     1441 tgattaaatc agtctttttt tattttatgt aataggattt tttattaata aaaaaatgta
     1501 ctataataaa aacattcgat aaaatcatga tattttattg acagtagaaa aatatcggta
     1561 tatcatatag ccaagaaaac cgatgaagtg gagataaaaa tagatcgtgc acttatagag
     1621 agcttggatg gtggaatcaa gnnnnnnnnn nnnnnnnnaa atggaccact gagggcaaag
     1681 tgaaaagcgt tagccgagta ttctttgacg ttagacttac gtcagtagtc atagatgtga
     1741 tagcatctac taagagcacg attgtatcgt gaaccaaggt ggcaccgcgg aatatatatc
     1801 cgtccttgac agaaattatt ttctgtcggg acggtttttt atttacaaag gaggaaatta
     1861 gcgatgtatt atccaacatt
//
LOCUS       DAOMTA010000659         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3101_length_1878_cov_1.799232, whole genome shotgun sequence.
ACCESSION   DAOMTA010000659 DAOMTA010000000
VERSION     DAOMTA010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_1878_cov_1.799232"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>311)
                     /locus_tag="PLK68_14165"
     CDS             complement(<1..>311)
                     /locus_tag="PLK68_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HRM91593.1"
                     /translation="RCPNCGNPTKYEKKTDVTKKYASSFNINNSTNNKDCDKHSEKSH
                     VYHQQEMYDYKSSNNEIKQRLEEARQRINSMGFPIIKKKAANNEEKLRNIIVGIVVIL
                     IA"
     assembly_gap    312..321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(554..1570)
                     /locus_tag="PLK68_14170"
     CDS             complement(554..1570)
                     /locus_tag="PLK68_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRM91594.1"
                     /translation="MLTMGFIGNGKSTNRYQLPFVLTRKNIKVKTIYARNLNKHDWSR
                     VAGINYTDDLDSLLNDPEIQLISVCTRHDSHYEYARMVLEHGKNCLVEKPFMKNSAEA
                     KEIFALAKEKGLLVQCYQNRRYDSDFLTVQKVIESGKLGNLLEVEMHYDYYRPEIPLN
                     VHEYVGYNGYLYGHGCHTLDQVISYFGKPEKIHYDVRQLLGSGRMNDYFDLDLYYGTL
                     KVSVKSSYFRVKERPSFVVYGDKGCFVKATKDRQEEHLKLFYMPGSPNFGIDRPEDYG
                     TLSYYDDAGVFHEEKVISEVGDYGNVFDGLYESIIEGKEPRVKDEQTLLQMEILETGV
                     KMCK"
     gene            complement(1656..>1878)
                     /locus_tag="PLK68_14175"
     CDS             complement(1656..>1878)
                     /locus_tag="PLK68_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM91595.1"
                     /translation="PLLVLFSCAIVNLPPLVTAVATVLAGMPAGSTTAILAEKYDGDS
                     NLAVEIVFLSTALSLFTIPLLCLVINMVV"
BASE COUNT          599 a          376 c          216 g          677 t
ORIGIN      
        1 gcaatcaata tcactacaat tccaactata atatttctta gtttttcttc attattagca
       61 gccttctttt ttatgattgg aaagcccatg ctgttaattc gctgtctagc ttcttctaaa
      121 cgttgtttga tttcattatt gcttgatttg taatcataca tctcttgttg gtggtaaaca
      181 tggctttttt cactatgttt atcgcagtct ttattatttg ttgaattatt tatgttaaaa
      241 ctactggcgt acttcttagt gacatcagtc ttcttttcat actttgttgg attcccacaa
      301 ttagggcatc gnnnnnnnnn ntggttgacc aaaatgtatt gctgctccac aaactgagca
      361 atgacttatc ttcccctgac gatgaccaca taacggacat tttaaactat ttttatactc
      421 atttccgcaa ttatcacatc tcattgtacc tcacccccta ttaattatat tgtaccatgc
      481 ccctatttag agtcaatcag ccaaataata taaataattt ttatcaaaaa taaaaagata
      541 ctgtcgtatc ttcttactta cacattttaa ctccggtttc taatatttcc atttgcaata
      601 aagtttgttc atctttgacc cgaggttcct taccctcaat aatactttca tataagccat
      661 caaaaacatt tccataatcc cctacttcag agatgacttt ttcttcatga aaaacacctg
      721 catcatcata atacgataac gttccataat cttcaggacg atcaattcca aaatttggtg
      781 accccggcat ataaaataac ttgaggtgtt cttcttgacg atcttttgtc gctttaacaa
      841 agcatccttt atcaccataa acaacaaaac taggacgttc ttttactcta aaataacttg
      901 actttaccga aactttaagt gttccataat atagatccaa atcaaaataa tcattcattc
      961 gtcctgatcc taataattgt cttacatcat aatgaatctt ttcaggttta ccaaaataag
     1021 aaattacttg atctaaagta tgacatccat gaccatataa atatccattg tacccaacat
     1081 attcatgaac atttagtggt atttccggac gataatagtc ataatgcatt tctacttcta
     1141 ataaattacc taattttcca ctttcgatca ctttttgaac tgttaaaaaa tcagaatcat
     1201 aacgacggtt ttgatagcat tgcactaata accctttttc tttagctaat gcaaatattt
     1261 ctttagcttc agcactattt ttcataaaag gcttttcaac caaacaattt ttaccatgtt
     1321 ctaaaaccat tctagcatac tcataatgac tatcatgacg agtgcaaaca ctaatcagct
     1381 gaatctctgg atcgtttaat agactatcta aatcatcagt ataatttatt cctgcaacac
     1441 gtgaccaatc atgtttattt aaatttcgag cataaatagt ttttacttta atattttttc
     1501 ttgtcagtac aaatggcaat tgataacgat tagtactttt accattgcca ataaatccca
     1561 ttgttaacat aatcttctcc ttctactttg atatatcata ttgtactata ttctaatcat
     1621 taattatatt ttttacgata attgaaaaga tgattttaaa caaccatatt aataaccaaa
     1681 cataataacg gaattgtaaa taatgatagc gctgttgata aaaagacaat ttctactgcc
     1741 aaatttgaat ctccatcata cttctcagcc aaaattgctg tcgtacttcc tgctggcatt
     1801 cccgccaaaa ctgttgctac tgcagttact aatggtggta gattaactat tgcacaacta
     1861 aataagacca ataaagga
//
LOCUS       DAOMTA010000660         1874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3107_length_1874_cov_2.800990, whole genome shotgun sequence.
ACCESSION   DAOMTA010000660 DAOMTA010000000
VERSION     DAOMTA010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_1874_cov_2.800990"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLK68_14180"
     CDS             <1..413
                     /locus_tag="PLK68_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91596.1"
                     /translation="DNEIIELLNKLLGEISSVKKMNIENNHILNEIGKRFYNIGKSKF
                     DINDEQIEKLKEVLREIGYEIENVNHENKKNFQIILQHINTFETNKNNKISKYKDHII
                     EYAKNNIFRFTVICSSISIFITIVLIKIMEVFVK"
     gene            410..>1874
                     /locus_tag="PLK68_14185"
     CDS             410..>1874
                     /locus_tag="PLK68_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91597.1"
                     /translation="MMMMGLFSGISEDVANFFRDFLWKLIETEIDCVRNLINALVNGV
                     LSFGILDNTWIQDGYKAAITLMFIILPAKIIYEFIFAMVREDDNASDVNKKLGGAIMG
                     VMLAVSLSIVVPLANNLSINVSKALIGQQYSVGSSSNGNSWNDNSGTSLNNSSSASND
                     TNQKDLAKELVVSTLCAFGGMDRNNTTFSYVTNEDGVGMDIGAERFYDYITNSDGALA
                     GYEGEPSENSGVRSSDNFFSKGIYNRWSFYYRWDVNGNGTDGSDDWDLLGSWDSSSSF
                     PESSKSDGGYGSRSVPSNETQAKQAYEYICDHAGDYIWDFGYIGTMIGLAIFMILLFI
                     ITIEVAMRIIMIGFLYVIGPLCCLSLTNYQNPQAFTVWKNTILGIFFVNVTQIFMLQL
                     LMNLAGDISKAGSGNSNVIASIALYFGTFSAIIGLPKYVQSMIGGYGQGIMESMQQLK
                     GAVGGAWGMTGGAVLGAGRKVMGRHNDNTGHLTGGLGG"
BASE COUNT          636 a          217 c          403 g          618 t
ORIGIN      
        1 aagataatga aattatcgag ctattgaaca aactgcttgg agaaatttcc tctgttaaaa
       61 agatgaatat agaaaataat catattttaa atgaaattgg taaaagattt tacaatattg
      121 gaaaatcaaa atttgatata aatgatgaac agatcgaaaa attaaaagaa gtgttaagag
      181 aaataggata tgagattgaa aatgtaaacc atgaaaataa gaaaaatttt caaattattt
      241 tacagcacat aaacacattt gaaaccaata aaaataacaa aataagtaag tataaagacc
      301 atattataga atatgctaaa aataatatat tcagatttac agttatatgc tcatccatca
      361 gtatatttat tacgattgtt ttaataaaaa ttatggaggt atttgtaaaa tgatgatgat
      421 gggcttattt agcggaatca gcgaggatgt agctaatttt tttagagatt tcttatggaa
      481 actgattgaa actgaaatag actgtgtcag aaatttaatt aatgcacttg ttaatggcgt
      541 attatcattt gggattttag acaatacatg gattcaggat ggatataaag ctgcaattac
      601 tctcatgttt attattttgc ctgcaaaaat aatttatgag tttatttttg cgatggttcg
      661 agaagatgat aatgctagtg atgtgaataa gaagcttggt ggagcgatta tgggagttat
      721 gcttgcagtt tccttaagta tagtagttcc tctggctaat aatctgtcta taaatgtatc
      781 taaagccctt attggtcaac agtacagcgt tggaagcagt tcgaatggaa attcttggaa
      841 tgataacagc ggtacatctt taaataattc atcatcggca tctaatgata caaatcaaaa
      901 agatttagcg aaagagttgg ttgtttctac tttatgtgca tttggcggta tggatagaaa
      961 taatacaaca ttttcttatg taactaacga agatggagta ggaatggata taggtgctga
     1021 aagattttat gactatataa ccaacagtga cggtgctttg gcaggatatg aaggtgagcc
     1081 tagtgaaaac agtggtgtaa gatcatcaga taattttttt tcaaagggaa tttataaccg
     1141 atggtcattt tactatcgtt gggatgtaaa cggtaatggg actgacggta gcgatgactg
     1201 ggatcttctt ggcagttggg attcatcatc atcttttcca gagtcatcaa agtctgatgg
     1261 cggttatggt tcaagaagtg tcccgtcaaa tgaaacgcag gctaaacagg catacgaata
     1321 tatttgcgac catgcaggag attatatctg ggactttggt tatattggaa caatgatagg
     1381 actggcgata tttatgatac ttctttttat tattacaatt gaagtagcaa tgagaattat
     1441 tatgattgga tttttatatg taattggacc gttgtgttgt ttatcactta caaattatca
     1501 aaatccgcag gcgtttacag tatggaaaaa taccatttta ggtatatttt ttgtaaatgt
     1561 gactcagatt tttatgttac agcttttaat gaatctagct ggagatattt ctaaagcagg
     1621 aagcggaaat agtaatgtta ttgcttcgat tgcactgtat tttggtactt tttctgcaat
     1681 tattggtttg cctaaatatg ttcagtcaat gattggaggt tatggacaag gaataatgga
     1741 aagtatgcag cagctaaaag gtgcagtagg tggtgcatgg ggtatgactg gaggtgctgt
     1801 tttaggagct ggaagaaagg tcatgggtcg acataatgat aatactggtc atttaactgg
     1861 cggattgggt gggg
//
LOCUS       DAOMTA010000661         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3109_length_1873_cov_2.334433, whole genome shotgun sequence.
ACCESSION   DAOMTA010000661 DAOMTA010000000
VERSION     DAOMTA010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_1873_cov_2.334433"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="rsmH"
                     /locus_tag="PLK68_14190"
     CDS             complement(<1..470)
                     /gene="rsmH"
                     /locus_tag="PLK68_14190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM91598.1"
                     /translation="MFDHVTVLLKEAVEGLNIKADGIYVDCTLGGAGHSCAILKQLTT
                     GHLYCFDQDQTAIEAARLRLDAVGNNYTIIYSNFANIKEKLNELGVTRVDGILFDLGV
                     SSPQFDTGSRGFSYNYDARLDMRMDTSNPLDAYAIVNTWPYERIVEILYKYADE"
     gene            complement(480..911)
                     /gene="mraZ"
                     /locus_tag="PLK68_14195"
     CDS             complement(480..911)
                     /gene="mraZ"
                     /locus_tag="PLK68_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRM91599.1"
                     /translation="MFMGEFRHNIDAKGRLIIPSKLREQCGESVVITRGFDGCLALYT
                     QEGWNDYYQKLQTLPKTKREARNFVRIITSRASECEFDKLGRVNIPNVLRIEGKLEKE
                     CIIVGVGDHVEIWNQNIWDDYYDANKDNFDEISESLEGFEL"
     gene            complement(1109..1276)
                     /gene="rpmF"
                     /locus_tag="PLK68_14200"
     CDS             complement(1109..1276)
                     /gene="rpmF"
                     /locus_tag="PLK68_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRM91600.1"
                     /translation="MAVPFRRVSSTRRNKRRTHDKLTAPAVVVCPECGEYKMSHKVCK
                     HCGTYKGQKVL"
     gene            complement(1293..1805)
                     /locus_tag="PLK68_14205"
     CDS             complement(1293..1805)
                     /locus_tag="PLK68_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRM91601.1"
                     /translation="MIILKYNLQWIVKHRGKFDFEEGLTFPSELFDQYAQINDLKDII
                     VSGTGDLDLKDKRLYVDLNIKGTMILPCAITLEDVEYPFEINSTEVFAFEKPDPLEDV
                     HEVKKDIVDLTPVVFENIMLEVPMRVVKDDANIKSKGKGWRILDNSTSDKDDDYIDPR
                     LAKLKDYFKD"
BASE COUNT          618 a          400 c          227 g          628 t
ORIGIN      
        1 ttttcatcag catatttata taaaatttca acaatccttt catacggcca cgtattaacg
       61 attgcataag catcaagtgg atttgatgta tccattcgca tatcaaggcg agcatcataa
      121 ttatagctaa agcctctaga acctgtatcg aactgaggag atgatacacc taaatcaaac
      181 aatattccgt cgactcttgt tacacctaat tcatttaatt tttctttaat atttgcaaaa
      241 ttagaataaa tgattgtata gttgttacca actgcatcaa ggcgtagacg agctgcttca
      301 attgccgttt gatcttgatc aaaacaatac aaatgaccgg tcgtaagctg ctttaaaata
      361 gcacaactat gtcctgctcc gcctaaggtg caatcaacat aaattccatc tgctttaata
      421 ttcaagccct caacggcttc cttcaataat actgttacat gatcaaacat cgctctctcc
      481 tataattcaa aaccttctaa cgattcagaa atttcatcaa agttatcttt attggcatca
      541 taataatcat cccaaatatt ctgattccag atttctacat gatcaccaac gccaacgatg
      601 atacattcct tttctaattt accttctatt cttaaaacat taggaatatt tactctccct
      661 aatttatcaa attcacattc acttgctctt gaagtaataa ttctaacaaa gttacgtgcc
      721 tctcttttag tttttggcag tgtttggagt ttttgataat agtcattcca gccttcttgg
      781 gtgtataaag ctagacaacc atcaaaaccg cgagtgatta caacactctc gccacattgc
      841 tcccttaatt ttgaaggaat aataagacgc cccttggcgt caatattatg cctaaattca
      901 cccatgaaca tctatatcac ccactcttta acacttagta acacaaatta acacttctta
      961 ccaccattat ataccctggt cgatactttg tatagcattt tctttaaata agaagatttc
     1021 gacaattaaa caaaaaaggg tcaattaacg taaaatttcg ttaatcaacc cttaaacaac
     1081 aaaaaaagcg cccttaagac gccgtttctt ataaaacttt ttgtcctttg taagtaccgc
     1141 aatgtttaca aactttatga gacattttgt attcaccgca ctctggacat acaactacag
     1201 ctggtgctgt aagcttatca tgagttcttc tcttatttct tcttgtactt gaaactcttc
     1261 taaatggtac tgccatcagt ataacctcct atttaatctt taaaataatc ttttaattta
     1321 gctaacctag gatcgatata atcatcatct ttatcactag tactgttatc taatattctc
     1381 caaccttttc ctttagattt aatattagca tcatctttga cgaccctcat cggtacttcc
     1441 agcataatgt tttcaaatac cactggggta agatcaacaa tatctttttt tacttcatga
     1501 acatcttcaa gtggatcagg tttttcaaaa gcaaacactt cagttgaatt aatttcaaaa
     1561 ggatactcaa catcttcaag tgtaatagca caaggaagaa tcatagttcc tttaatatta
     1621 agatcaacat ataaacgttt atctttcaaa tcaagatctc cggttcctga gacaataatg
     1681 tccttcaagt cattaatttg agcatactgg tcaaataact ccgaaggaaa agttagtccc
     1741 tcttcaaaat caaattttcc acgatgctta acaatccatt gtaaattgta tttcaaaata
     1801 atcacccttc tataaccgca atgacaatta tatataaaat aataccataa gtcaacctaa
     1861 aaaatacttg ttt
//
LOCUS       DAOMTA010000662         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3111_length_1873_cov_2.305831, whole genome shotgun sequence.
ACCESSION   DAOMTA010000662 DAOMTA010000000
VERSION     DAOMTA010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_1873_cov_2.305831"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..939
                     /locus_tag="PLK68_14210"
     CDS             160..939
                     /locus_tag="PLK68_14210"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HRM91602.1"
                     /translation="MDTYTNQSQMIEYSLDDFQWGKDPFYIRIKNNFFTKEQIILDLD
                     NGLVDIQGNLKNSQYTKLETTCYAPTIMGPFHYLPFLECNHAIISLRHHITGSLKVNN
                     QKFQIIGDGYIEKDWGRSFPQDYLWLQSNSCKEKEASLFLSIAKIPLLACSFQGLIMN
                     LLVDDQQIRVATYYGARVKDMFTREGYHYLIISQHPHTFYLKIKAGHRFELKSPQSGK
                     MNGYVEESLNALAVLLVYKKNKKVAKFNFINCGFELFGNWL"
     gene            1023..1547
                     /locus_tag="PLK68_14215"
     CDS             1023..1547
                     /locus_tag="PLK68_14215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRM91603.1"
                     /translation="METIITERLILRSPDNNDAQTLLKIHNDMATLKFNAMTKYEFAE
                     MIRDIERKHDMMYCIELKNSNNVIGAVFINPDRLRYRVNALSLSYYLDCRYYRNGYMY
                     EALQAIIIKLFEQEIDIISARVFKENTASARLLEKLGFQYEGCLRMGVTGYQEIVHDD
                     FFYSILKDEVNLTV"
     gene            1623..>1873
                     /locus_tag="PLK68_14220"
     CDS             1623..>1873
                     /locus_tag="PLK68_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRM91604.1"
                     /translation="MLKVWVVGSNGQIGTAINEVIEPLEIEVLNTDQDELDITNTDDV
                     ISFGEINRPDIIINCAAITDVYKCERNRDQAFRVNALGPR"
BASE COUNT          676 a          204 c          354 g          639 t
ORIGIN      
        1 atgaaaatag aaaatacaga acttaattta catcttaaag acagtgacca gcgcttgttt
       61 gaaggatggt atttcaaaat tgttgattga aaaatttctt tagcaattat tgtcgggatt
      121 agtaagacaa ttgaaaaatc ttgtgcattt attcaaactt tggatacata tacaaaccaa
      181 agtcagatga ttgaatatag tttagatgat ttccagtggg gaaaagatcc cttttacatt
      241 agaattaaaa ataatttttt tactaaagaa caaattattt tggatcttga taatgggcta
      301 gttgatattc aaggaaactt gaaaaatagt cagtatacta aactagaaac aacatgttat
      361 gctccaacga ttatgggacc ttttcattat ttaccatttt tagaatgtaa tcatgcaata
      421 ataagtctgc gacatcatat tactgggagc ttaaaagtaa ataatcagaa atttcagata
      481 attggtgatg gctatattga aaaggactgg ggacgttcat ttccacaaga ttatttatgg
      541 ttacaatcaa atagctgtaa agaaaaagag gcaagtttat ttttatcaat tgctaagatt
      601 ccactattag cgtgtagttt tcaaggattg atcatgaact tattagtaga tgatcaacaa
      661 attcgagtag caacttatta tggagcaaga gtaaaagata tgtttacgcg ggaaggatat
      721 cattatttaa ttattagtca acatcctcat acattttatt tgaaaataaa agcaggacat
      781 cgttttgaat taaaatcacc acaatcagga aagatgaatg gttatgttga agaaagtctt
      841 aatgctttag ctgttttatt agtttataaa aagaataaaa aagttgctaa gtttaatttt
      901 attaattgtg gctttgaatt gtttggaaat tggttataag atgattaatt atcatctttt
      961 tttatttgtc gtggtacaat atgagtatat aataatgagt tatttggata agggagaaaa
     1021 taatggaaac aataataaca gaacggttga ttttaagaag tcccgataat aatgatgctc
     1081 aaactctttt aaagattcat aatgatatgg caacgttaaa gtttaatgcg atgacgaaat
     1141 atgaatttgc tgagatgatt agagatattg agaggaaaca tgatatgatg tattgcatag
     1201 aactaaaaaa ttctaataat gttattggtg ctgtgtttat aaatcctgat cgtttaagat
     1261 atcgtgtaaa tgctctcagt ttatcatatt atttagactg taggtattat cgtaatggct
     1321 atatgtatga agctctgcaa gctattatta taaaattgtt tgagcaagag attgatatta
     1381 ttagtgctcg agtgtttaaa gaaaatactg cttctgcaag gttgttagaa aaactaggat
     1441 ttcagtatga aggttgtctt cgtatggggg taaccggtta tcaagagatt gttcatgatg
     1501 atttctttta ctcaatttta aaagatgagg taaatttgac agtataaaat ttcattgcta
     1561 taataaaaaa agattagcgt tgatacattc agcgctttta ttatgaaaag aggaggaaaa
     1621 ttatgttaaa agtatgggtt gttggttcca atggccaaat cggaacagcg attaatgaag
     1681 taattgaacc attagaaata gaagttttaa atacagatca agatgaatta gatattacta
     1741 atacagatga tgtgattagt tttggagaaa tcaatcgacc ggatattatc attaattgtg
     1801 ctgcgattac cgatgtttat aagtgtgaaa ggaatcgtga tcaagctttt cgggtcaatg
     1861 ctttaggacc acg
//
LOCUS       DAOMTA010000663         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3113_length_1873_cov_2.067107, whole genome shotgun sequence.
ACCESSION   DAOMTA010000663 DAOMTA010000000
VERSION     DAOMTA010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3113_length_1873_cov_2.067107"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>842
                     /locus_tag="PLK68_14225"
     CDS             <1..>842
                     /locus_tag="PLK68_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRM91605.1"
                     /translation="SVIGEDGNTFEIQIRTEEMDELAELGVAAHWRYKENKGYSSKKE
                     QQEIGEKLQWLREFISLSDDIKDGDAKEYYDSLKRDIFEANVYVLTPQGKIVELPNGS
                     TPIDFAYRIHTEVGHKAVGAIVNNVMVPIDTKLNTGDVVEIKTNKQSLGPSEDWLKFV
                     RTAGARNKIRQFIANREAETKKETIEDGRKMLRDELKKRQLDEKKYMDPDTYKTYLGS
                     FGARSFDDILYFIGKKSLPAMNLLDRVVPKKSGFFDSISKMLQRNNQVNEKQQSSRNN
                     SGVVV"
     assembly_gap    843..852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <853..1281
                     /locus_tag="PLK68_14230"
     CDS             <853..1281
                     /locus_tag="PLK68_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HRM91606.1"
                     /translation="KGLDGLRIQLSKCCNPIPGDDIMGFVSQGQGIKVHRRDCPNIQQ
                     PEIKARLIDVYWDFASISTMKFQADLEIVGLDRPNLLNDVVTSLGQMKINILNIHADV
                     VDMKAIIKLKISVEDASRLQQAIDNIDRIQGIYEIKRVIH"
     misc_binding    1317..1518
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1557..>1873
                     /locus_tag="PLK68_14235"
     CDS             1557..>1873
                     /locus_tag="PLK68_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRM91607.1"
                     /translation="MMYKAPRGTVDILPEKSAKWQTLEQLIRTICANYNVKEVRTPIF
                     EHTELFTRAVGDTTDVVSKEMYTFADKKGRSITLRPEGTAGVARAYVENKMFSNPDKI
                     SKL"
BASE COUNT          658 a          245 c          399 g          561 t
ORIGIN      
        1 tcagtaattg gtgaggatgg aaataccttt gagattcaga ttagaactga agaaatggat
       61 gagctagctg aattaggagt tgctgctcat tggcgctata aggaaaataa aggctattca
      121 tcgaagaaag aacaacaaga aattggtgaa aaattgcagt ggcttagaga atttatttct
      181 ctaagtgacg atattaaaga cggcgatgct aaagagtatt atgattcatt aaagcgtgat
      241 atctttgagg ccaatgttta tgttttaaca ccgcaaggaa agatcgtaga gttaccgaat
      301 ggttctacac caattgattt tgcttaccga attcatacgg aagttggaca taaagcagtt
      361 ggagctatcg ttaataatgt tatggtgcct attgatacta aattaaatac tggtgatgta
      421 gtcgaaatta aaactaataa gcagtcatta ggacctagtg aagattggct aaaatttgtt
      481 cgaactgcag gtgcgcgtaa taaaattaga cagtttattg ctaatcgtga ggcagaaact
      541 aaaaaagaga caattgaaga tgggcgtaaa atgcttcgtg atgaattgaa aaaacgccaa
      601 ttagatgaaa agaaatatat ggatccagat acgtataaaa catacttagg ttcttttggt
      661 gcccgctcat ttgatgatat cttatatttt attggtaaaa aaagtttacc ggcaatgaat
      721 ctgctggatc gagttgtacc taaaaagtcg gggttctttg acagtatttc gaaaatgctt
      781 cagcgaaata atcaggtaaa tgaaaaacag cagtcttcgc gtaataattc gggtgttgtt
      841 gtnnnnnnnn nnaagggatt agatggacta agaattcaac tttcaaagtg ctgtaatcca
      901 attcctggag atgatattat gggctttgtc tcacaaggtc agggaattaa agtacatcgt
      961 cgagactgcc cgaatattca acagcctgaa atcaaagcaa gattgataga tgtttattgg
     1021 gattttgcat ccatttcaac gatgaaattt caagcagatt tagaaatagt tggtttggat
     1081 cgacctaatt tattgaatga tgtggtaaca tcattagggc agatgaaaat taatattttg
     1141 aatatccatg ctgatgttgt agatatgaaa gcaatcatta aattaaagat ttcagttgaa
     1201 gatgctagtc gtttacaaca agcaatcgat aatattgatc ggattcaggg aatctatgaa
     1261 attaaacggg ttattcatta gttattgcaa ttttatgaaa tattatatat aatcaattta
     1321 atccaatgaa caagattaag tagttaaatc cttttataga gagaatacgt ctggtgaaag
     1381 tatttaaagg atcattacgg gacacttggg aggagttact ttgaaatcta gtaggagtaa
     1441 acgtctgtcg cgttaagaca taagagcgtg ttatttaaca cgaattaagg tggtaccgcg
     1501 ttaactaacg tccttatttt aagggcgttt ttatattatc aaggaggaaa tgaaagatga
     1561 tgtataaagc accacgagga acagtggata ttttgccaga aaagagtgct aaatggcaaa
     1621 cattagaaca actaattaga acaatatgtg ctaattataa tgttaaagaa gtgagaactc
     1681 ctatttttga acacaccgag ttattcactc gtgcagttgg cgatacaact gatgtagtaa
     1741 gtaaagaaat gtatactttc gctgataaaa aggggcgttc aattacatta cgacctgaag
     1801 gaacagctgg agttgcgaga gcttatgtag aaaataaaat gttttctaat cctgataaaa
     1861 ttagtaaatt ata
//
LOCUS       DAOMTA010000664         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3114_length_1873_cov_1.814631, whole genome shotgun sequence.
ACCESSION   DAOMTA010000664 DAOMTA010000000
VERSION     DAOMTA010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_1873_cov_1.814631"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1387)
                     /gene="nusA"
                     /locus_tag="PLK68_14240"
     CDS             complement(56..1387)
                     /gene="nusA"
                     /locus_tag="PLK68_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRM91608.1"
                     /translation="MASKKFMDALNLLIEEKGIEKDVFLEMLKESIGKAYKKNYLNPD
                     ANVRVEINEKTGKFRLFELRTVVDDLDDEDIELSLEEAQAINPNYQIGDVVETEADIE
                     HIGRLAAIQTKQLFRQKIRETEKETLYNEFADKKDDIITGIVDRVEDKFAIVNIGKTG
                     AFLASNQQIPGEKLNEGQHLKVYVSDVDRGTKGTHIVVSRTEPSFVKRLFEVYDGTVE
                     IKAVSREPGERSKVAVYTSNENIDPIGSCVGPKGSRVKNVVDELNGEMIDIILWSSDP
                     VVFISNALSPSDVKWVSINEENHSALVVVPDDQLSLAIGKRGQNARLAVRLTGWKIDI
                     KSVSEAVELGLIDLQTVNNTEESSPVDASFEEEFAQEMLDEAVEEAVVEEAVEVVEEP
                     EIEEEVVEVEEEPTQSKKVIEYEDFEDLDDEYSKYDEEIDYDEYDEYYDKD"
     gene            complement(1400..>1873)
                     /gene="rimP"
                     /locus_tag="PLK68_14245"
     CDS             complement(1400..>1873)
                     /gene="rimP"
                     /locus_tag="PLK68_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRM91609.1"
                     /translation="ERMELLDTIKTLIKPILENNDVYLDDIEYLQENGEWYLRIFVEK
                     NEGSLDMDTCVAVSEAISLKMDEEDPIKGEYYLEVSSPGVEKPLKTFEQVKASVGKYV
                     YAKFINPTAGMDEVEGFIKTIEDETIEFEYLVKNIKKRIKIDYSNIKFIRLAVKF"
BASE COUNT          568 a          401 c          205 g          699 t
ORIGIN      
        1 gttcacccgt tgctaaacat tttcttaaag ggatttttcg caatttaatc actccttaat
       61 ctttatcata gtattcatca tattcatcgt aatcgatttc ttcatcatat ttactatatt
      121 catcatctaa atcttcaaaa tcttcgtatt caataacctt tttcgattga gtaggttctt
      181 cttcaacctc aacaacttct tcttcgattt ctggttcttc tacaacttca acagcttctt
      241 ccacaacagc ttcctcaact gcttcatcta acatttcttg agcaaactct tcttcaaaac
      301 tagcatcaac aggtgatgat tcttctgtat tattaactgt ttgaagatca attaaaccaa
      361 gttcaacagc ctcactaaca gatttaatat caatcttcca acctgttaaa cgtactgcta
      421 aacgagcgtt ttgaccacgc ttaccgatag ctaaagataa ttgatcatct ggtacaacta
      481 ctaaagcact gtgattttct tcattaatag atacccactt aacatcagat ggactcaagg
      541 cattagaaat aaagacaacc ggatcactac tccataaaat aatatcaatc atctcaccat
      601 ttaactcatc aactacattt ttaactcgac ttccctttgg tccaacacaa gagccaattg
      661 gatcaatatt ttcattacta gtataaacag caaccttact tctttcacct ggttcccgtg
      721 aaactgcttt gatttcaact gttccatcgt atacttcaaa taatctctta acaaaactcg
      781 gctctgtccg gctaacaaca atatgagttc ctttagttcc acgatcaaca tcgctaacat
      841 acacttttaa atgttgaccc tcatttaatt tttcaccagg aatttgttga ttagaagcta
      901 agaaagctcc tgttttacca atatttacaa ttgcaaattt atcctcaaca cgatcaacga
      961 tacctgtgat gatatcatct tttttatctg caaattcgtt ataaagtgtt tctttttcag
     1021 tttcgcgaat cttttgtcta aataattgtt ttgtttgaat agctgccaaa cgtccaatat
     1081 gttcgatatc ggcttcagtt tcaacaacat ctccaatttg ataattagga ttgattgctt
     1141 gagcttcttc tagagataat tcaatatctt catcatctag atcatcaaca actgttcgaa
     1201 gttcaaataa tctaaacttt ccagtttttt cattaatttc aacacggaca ttagcatcag
     1261 ggtttaaata atttttctta tatgctttac caatagattc tttaagcatt tctaaaaaga
     1321 catctttttc aatccctttt tcttcaatta ataaatttag tgcatccatg aattttttac
     1381 ttgccacttt tttatcctcc taaaatttca cagctagtct tataaattta atattgctat
     1441 agtcaatttt aattcttttc ttaatattct taactaaata ttcaaattca atcgtttcat
     1501 cttcaattgt tttaataaat ccttccactt catccattcc agcagttgga ttaatgaatt
     1561 tagcgtagac atacttacct actgaagctt taacttgctc aaaagtcttt aggggctttt
     1621 ctacacctgg tgaagaaact tctaaataat attctccttt gatcggatct tcttcatcca
     1681 tctttaaact aattgcttca ctaactgcta cacatgtgtc catgtctagg gaaccttcat
     1741 ttttttcaac aaaaatgcga agataccatt cgccattttc ctgaaggtat tcaatatcat
     1801 caagatatac atcattattt tctaaaattg gttttatcag tgttttaatc gtatctaaaa
     1861 gttccattct ttc
//
LOCUS       DAOMTA010000665         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3117_length_1872_cov_2.725371, whole genome shotgun sequence.
ACCESSION   DAOMTA010000665 DAOMTA010000000
VERSION     DAOMTA010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_1872_cov_2.725371"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLK68_14250"
     CDS             <1..1450
                     /locus_tag="PLK68_14250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM91610.1"
                     /translation="NENKVHDLVLKKKIELDPLTGLYNREAIEIKTNEIIQNFPENNH
                     AIMILDIDNFKQVNDNFGHLYGDAFLCEISRKIKSKFRNDDLVARLGGDEYLVLMKNI
                     SNSELAIDKANDLCKLIEGAYGTGGTKVKVTVSVGVVLYPNHGKTFDELYHHGDQALY
                     KIKKNNKNGVCLYDESLEISDGQLDKVNNIVDRATKKFSDNVGEYIFRILYKCQDLNE
                     TITAVLELLGMHYYMSQNLIIVRDLDIGKYKVNNYWSSKNEDLTKVIDVTYHDYWPEY
                     VRQFDDEGILWVNDIKASDVSPAIKTLYANSKTRMVISGLIKNGDDIIGIISMEHEKP
                     YAYKAEEKEMLLTSIAIISTFLVKKYQEKEKNQYLNAIQMILDFQENGIYVIDPNTYK
                     LVYYNHKIKHIFNQVKVGDYCYKSFRGYDEPCADCPIKDMKDSDLSYTKLIYNCNISA
                     QLETTVKRVRWINGQDVAAVTSIDITKYYNEK"
     gene            1492..>1872
                     /locus_tag="PLK68_14255"
     CDS             1492..>1872
                     /locus_tag="PLK68_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM91611.1"
                     /translation="MRIYFRGNLYGCNQATAFVEDKGKIIFIGSDKEALKYSGEQIDL
                     NNKYVYPGFNDSHMHLVNYGQSLKNVLLEKHTNSLKALLEELKKHLVKGQWLIGRGWN
                     HDYFTDEQRFPTRKDLDMISEEEPI"
BASE COUNT          687 a          201 c          363 g          621 t
ORIGIN      
        1 taatgaaaat aaagtacatg atcttgtact aaagaagaaa attgaattag atccactaac
       61 agggctatat aatcgagaag ctattgaaat taaaacaaat gaaattattc aaaattttcc
      121 tgaaaataat catgcgatta tgatcttaga cattgataat tttaagcagg ttaatgataa
      181 tttcggtcat ttatacggtg atgcattttt gtgtgaaata tctcgaaaaa taaaatctaa
      241 atttcgaaat gatgacttag ttgctcgtct tggtggtgat gagtatttag ttttaatgaa
      301 gaatatttca aacagtgaac tagctattga taaggctaat gatctatgta aactgattga
      361 aggtgcttat ggaactggag gaacaaaagt taaagtaacg gtatctgtag gggtagtttt
      421 ataccctaat catggaaaaa catttgatga gttatatcac catggtgatc aggctttata
      481 caaaattaaa aagaataata aaaatggtgt ttgtttgtat gatgagagtt tagagattag
      541 tgatggtcaa ttggataaag tgaataatat tgttgatcgg gctaccaaaa agttttctga
      601 taatgttggt gagtatattt ttagaatttt atataaatgt caagatttaa atgaaacaat
      661 tacagcagtc ctagaattat tgggaatgca ttattatatg agccaaaatt taattattgt
      721 acgagatttg gatattggga aatacaaagt aaataattat tggagttcaa agaatgaaga
      781 tttaacaaaa gttattgatg ttacttatca tgattattgg cctgagtatg tacggcaatt
      841 tgatgatgag gggattcttt gggttaatga tatcaaggct tcagatgttt ctccagcaat
      901 taaaacactt tatgctaata gtaagacccg tatggtaatt agtggtctta ttaaaaatgg
      961 cgatgatatt attggtatca taagcatgga acatgaaaaa ccctacgctt ataaagcgga
     1021 agaaaaagaa atgttattaa cttcgattgc tataattagt acttttcttg ttaaaaaata
     1081 tcaggaaaaa gaaaagaatc agtatttaaa tgcaattcaa atgattttag atttccaaga
     1141 aaatgggatt tacgttattg atcccaacac ttataaactt gtgtattata atcataaaat
     1201 caaacatatt tttaatcagg taaaagttgg tgattattgc tataagtctt ttagaggcta
     1261 tgatgaacca tgcgctgact gcccaattaa agatatgaag gatagcgatt tatcgtatac
     1321 taaattgatt tataattgta acattagtgc tcagttggag acaacggtaa aacgtgttcg
     1381 gtggatcaat ggtcaagatg ttgctgcagt tactagtatc gatataacaa aatattataa
     1441 tgaaaagtag gatcaaagta tcctactttc taaataaagg gggatttttt tatgagaatt
     1501 tatttccggg gaaatttata cggttgtaat caggcaactg cttttgtaga agataaggga
     1561 aaaattatct ttatcggaag tgataaggaa gcattaaaat attctggtga acaaattgat
     1621 ttaaataata aatatgtata tccaggtttt aatgattcgc atatgcattt agttaattat
     1681 ggtcaaagtt taaaaaatgt tctattagaa aagcatacta attcgcttaa agcactttta
     1741 gaagaattaa agaaacattt agtaaagggg cagtggctga ttgggcgggg ctggaatcat
     1801 gattatttta cagatgaaca gcgttttcct acccgaaaag atcttgatat gattagtgaa
     1861 gaggaaccga tt
//
LOCUS       DAOMTA010000666         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3125_length_1871_cov_1.746145, whole genome shotgun sequence.
ACCESSION   DAOMTA010000666 DAOMTA010000000
VERSION     DAOMTA010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_1871_cov_1.746145"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLK68_14260"
     CDS             <1..230
                     /locus_tag="PLK68_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRM91612.1"
                     /translation="ITKIRSNEVEFVIIASDASDNTKKKITDKCTSYKVEYVIACTID
                     ELSSAIGKKNRVALGIQDTGFAKILKEKIGG"
     gene            235..1642
                     /gene="infB"
                     /locus_tag="PLK68_14265"
                     /pseudo
     CDS             235..1642
                     /gene="infB"
                     /locus_tag="PLK68_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538247.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
     assembly_gap    1643..1652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1653..>1871
                     /locus_tag="PLK68_14270"
     CDS             <1653..>1871
                     /locus_tag="PLK68_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRM91613.1"
                     /translation="NDIRLASDKDTIIYGFNVRPNAKVRQKAEEEGIEFRLHNIIYKM
                     VEEIETAMKGMLAPEIKEVVTGQAEIRQV"
BASE COUNT          661 a          249 c          415 g          536 t
ORIGIN      
        1 taatcacaaa gattagaagt aacgaagtag aatttgttat tattgctagc gacgctagtg
       61 acaatactaa aaaaaagatc accgataaat gtacaagtta taaagttgag tatgttatag
      121 cttgtactat tgatgaacta tccagtgcaa tcggtaaaaa aaatcgagta gcacttggta
      181 tccaggatac tggatttgct aaaatattaa aagaaaagat aggagggtga acatatggca
      241 aaacaaaata aacaaacaaa aaataaaaaa ggtgcgccaa gaaagaaaca attaaagtct
      301 aattttccga ccaaaaaaga agaaatagtt gctaaagatg gtgtcattgt ttatgaagaa
      361 ggtattactg ttggtcaatt agctgataaa attggacaaa cgcctgccaa tgttattaaa
      421 gtattgtttc tattaggaac gatggtaact attaattcat ctttaaatga tgagcaagta
      481 gaattaattt gtcttgaata tggttttgaa gtcgaaaaac atgttgaagt tagtgaagtg
      541 aattttgagg aaattgatat tcaggatgat gagaaggatt tacagcctcg ttgtccagta
      601 gttacaatta tgggacatgt tgatcatggt aaaactacat tgttagatac aatcagaaag
      661 tctgcggttg tagaaggtga atttggtggg attacacaac atatcggtgc ttatcaagtt
      721 gaagttaatg gaaaaaagtt actttcttag atacaccggg ccatgaagca tttactgcta
      781 tgcgtgctcg tggagctcaa gtaactgata ttgttattat tgtagtagca gctgatgatg
      841 gtgttatgcc acaaacaaaa gaggcaattg atcatgcaaa agcagcaggt gtaccaattg
      901 tcgttgcgat taataaaatt gataaagaag gtgccgatcc tgaaagaatc aaaggagaaa
      961 tggctgagca tggtctgctt cctgaagatt ggggtggaga tacagtatac tgtgaaattt
     1021 ctgctaaaaa aagaattgga attgaagaat tgctagaaac tttaactgtt gttgcagaat
     1081 tagcagattt aaaagcaaat cctaatcgtt atgcatatgg cagtgtagtt gaaggtaaac
     1141 tagataaggg gcgtggacca gttgcaacgt tacttgttga aaatggtact ttaagagcgg
     1201 gagatccagt tgtagttgga acatcatttg gacgtgttcg tcaaatgctt gatgatcgtg
     1261 gaaaaattat taaagaagca cttcccgcta ctccagttga aattacgggt ttaaatgacg
     1321 ttccagtcgc tggtgataag tttatggctt ttgaaaacga aaaacaagct cgtagtgttg
     1381 gtgaaacacg attaaaagcg aaacaagata aagagcgtag ttcaggagct gccttatcat
     1441 tagatgattt atactctcaa atcaaagagg gcgaaatgat tgacttaaac attattgtta
     1501 aagctgatgt ccaaggtact gctgaagcag ttaaagcttc attagaaaaa attgatgttg
     1561 atggtgtaag agtaaatgtt attcgttcta cagctggagg aatttcagaa tctgatgttc
     1621 tattagcatc tgcttcacaa gcnnnnnnnn nnaatgacat tcgcttagcc tccgataaag
     1681 acactattat ttatggtttt aatgttcgtc caaatgcaaa agtacgtcaa aaggctgaag
     1741 aagaaggaat tgagtttcgt ttacacaata tcatctataa aatggttgaa gagattgaaa
     1801 ccgcaatgaa aggtatgctt gctccagaaa ttaaagaggt tgttacaggt caagcagaaa
     1861 ttcgtcaggt a
//
LOCUS       DAOMTA010000667         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3130_length_1869_cov_2.453142, whole genome shotgun sequence.
ACCESSION   DAOMTA010000667 DAOMTA010000000
VERSION     DAOMTA010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3130_length_1869_cov_2.453142"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLK68_14275"
     CDS             <1..265
                     /locus_tag="PLK68_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM91614.1"
                     /translation="FTTDGQLSTANVVVLEDDQQFFSTSMGALVVRNEILEQYPELNN
                     VFDKLTGILNETKMAQLNYLVETKGQDAEDVAHEFLVSINLVK"
     gene            275..1018
                     /locus_tag="PLK68_14280"
     CDS             275..1018
                     /locus_tag="PLK68_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91615.1"
                     /translation="MDTAIEFKHVKKVYGEKVIIDDFNLKITSGEFLTVVGSSGCGKT
                     TILKMINGLIIPDEGQVLVHDQCTQAVDLIELRRGIGYAIQGSVLFPHMTVAQNIAYV
                     PNLLNKNDKKRTYEALSKWMKIVGLDEELIHRYPSELSGGQQQRVGIARALAASPDIL
                     LMDEPFGAVDEITRSTLQDEILRIHHQENITIIFVTHDINEALKLGSRVMVMDQGKVV
                     QLASPREILEHPKTEFVRKLVQRKDDFLQ"
     gene            1152..1619
                     /locus_tag="PLK68_14285"
     CDS             1152..1619
                     /locus_tag="PLK68_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM91616.1"
                     /translation="MEKERYEEVVYQYSDMVTRIAVMNVKNYDDAKDCYQNVFMKLYC
                     YNKKFESEEHLKAWLIRVTINECKDYQKQFWKREIDIDKLILGQEDQRLIILPIVMNM
                     PSKYRNVLYLYYYEGYSTNEIASILKENHNTIKSQLIRGRKLLKKKLGDDFYE"
     gene            1612..>1869
                     /locus_tag="PLK68_14290"
     CDS             1612..>1869
                     /locus_tag="PLK68_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91617.1"
                     /translation="MNKFNEFDLIKTPRNWIDEVTATNFEPTKRAKVIRIKYIFAIVL
                     IVMTIGISSLGITYAFSDSFRTWLSQRFSSNLKISSALEFSS"
BASE COUNT          694 a          214 c          350 g          611 t
ORIGIN      
        1 ctttacaacc gatggtcaac tatcaacagc aaatgttgtt gtccttgaag atgatcaaca
       61 attcttttca acttcgatgg gggccttagt tgtacgcaat gaaattcttg aacaatatcc
      121 agaattaaat aatgtttttg ataaacttac tggtattctc aatgaaacaa aaatggcaca
      181 attgaactat ttggttgaaa caaaaggaca agatgctgaa gatgtggctc acgaattttt
      241 agttagcatt aatttagtga aataggagga acatatggat acagctattg aatttaaaca
      301 tgtaaaaaag gtttatggtg aaaaagtaat tattgatgat tttaatctaa aaattacatc
      361 tggtgagttt ttaactgtag ttggtagttc agggtgtgga aaaaccacta ttctaaaaat
      421 gatcaatggt ttaataattc ctgatgaagg acaagttctt gtacatgatc aatgtacaca
      481 ggcagttgat ttaattgaat taagaagagg aattggttat gcaattcaag gaagtgtctt
      541 atttccgcac atgacagtag ctcaaaatat tgcttatgtg cctaacttat taaataaaaa
      601 tgataaaaaa agaacttatg aagcactatc aaaatggatg aaaatagtag ggttagatga
      661 agagttaatt catcgatatc ctagtgaatt aagtggtggg cagcagcaac gagtagggat
      721 tgctagagca ctagctgctt ccccagatat tcttttaatg gatgaaccat ttggtgctgt
      781 tgatgaaatc acacggtcta ctttacaaga tgagatttta agaattcatc atcaagaaaa
      841 tatcacgatt atttttgtaa cacatgatat taatgaagct ttgaaattag gtagtcgggt
      901 tatggtaatg gatcaaggta aagtcgtaca attagcatca ccaagagaaa ttttagaaca
      961 tcctaaaaca gaatttgtta gaaaattagt tcagcgtaaa gacgattttc ttcaataaca
     1021 gccttttggg ctgttttatt ttactaaatc gataaaaaag taaaacaatc gattataatg
     1081 aaagtataaa aaataattat taatgcaccc gttgaacgtt tttaatcgta ttcatagtgg
     1141 aagggattgt tatggaaaaa gaaagatatg aagaagttgt atatcagtat agtgacatgg
     1201 ttactagaat tgctgttatg aatgttaaaa attatgatga tgctaaggat tgctatcaaa
     1261 atgtatttat gaaattatac tgttacaaca agaaatttga aagtgaggaa catttaaaag
     1321 cttggttgat tagggtaact atcaatgaat gtaaagatta tcaaaaacaa ttttggaaaa
     1381 gggaaattga tattgataag ttgattttag gtcaagaaga tcagcgttta attattctac
     1441 caattgtaat gaatatgcca agtaaatatc gtaatgtttt atacttgtat tattatgaag
     1501 gatattcaac taatgaaatt gcatctattt taaaagaaaa tcacaatacg ataaaatcac
     1561 aattaattag gggaagaaaa ttgttaaaga aaaagttggg agatgatttt tatgaataaa
     1621 tttaatgaat ttgacttaat aaaaactcct agaaactgga tcgatgaagt gactgcaact
     1681 aattttgaac cgacaaaaag agcgaaggtg attagaatta agtatatatt tgcaattgtt
     1741 ttaattgtta tgacaatagg aatttcttca ttgggaatta cttatgcatt ttctgattca
     1801 tttcgtactt ggttgagtca gcgatttagc agtaatttga aaattagttc agctcttgaa
     1861 ttttcttca
//
LOCUS       DAOMTA010000668         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3133_length_1868_cov_1.676227, whole genome shotgun sequence.
ACCESSION   DAOMTA010000668 DAOMTA010000000
VERSION     DAOMTA010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_1868_cov_1.676227"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLK68_14295"
     CDS             <1..902
                     /locus_tag="PLK68_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM91618.1"
                     /translation="KQIDYFIEKMMKAEIVVEKAHQDCLPTAFLSTVIDSCLEKGVDV
                     TRGGAKYNLSGIQMIQIANLADSLAAIKELVYDEKMITRHEELLAALQSDFKGYEIIQ
                     TMLLSKVPKYGNDVKWVDELGAKWAGYFRERMKDYTNYRGGLYHTGMYTVSAHVPMGE
                     NVGASPDGRNALTPLADGGMSPVYGRDMAGPTAVLKSVSRMKDSYTTNGGLLNMKFLP
                     EFFKTETGMMKFENFLRAFVDLKIPHIQFNVVRREDLLDAKLHPEQHRSLTIRVAGYT
                     AYFVELAGKLQDEIIERTAYEDI"
     gene            889..1806
                     /locus_tag="PLK68_14300"
     CDS             889..1806
                     /locus_tag="PLK68_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRM91619.1"
                     /translation="MKISKALVFDIKRFAVHDGYGLRTTVFFKGCPLRCKWCQNPEGL
                     SSQRRPIYFENSCIHCQRCVEFSKKNQIKYENNRPYFNLQYEGTFDNLVKACPGNAIR
                     YDSEAYDIKQLMEKIKEDRVFFRDDGGVTFSGGEPLMQGEFLYEILKACQEEKIHTAI
                     ETTMYGSLELIKKILPYLDLIYIDLKVFDEKRHMELTNVSSKMIKQHIEYILESEYRN
                     KVIIRTPLIPTMTATDHNIKSIANFLVNIYPEVKYELLNYNPLAFAKYELVDLEYEVD
                     KQLKMFDKEQMEHFHQLVYQTGLKNLIIE"
BASE COUNT          695 a          219 c          377 g          577 t
ORIGIN      
        1 aaaaacaaat tgattatttt atagaaaaaa tgatgaaagc tgagattgtt gtagaaaaag
       61 ctcatcaaga ttgcttgccg acagcttttt tatcaacggt cattgattct tgtttggaaa
      121 agggagtaga tgtaacacgt ggtggggcaa aatacaatct ttcagggatt cagatgatac
      181 aaattgcaaa tttagctgat tcacttgcag caatcaagga actcgtatat gatgaaaaga
      241 tgattaccag acatgaggaa ttactagcag ccttacaatc agattttaaa ggatatgaaa
      301 tcatccaaac gatgctttta agtaaagtac cgaagtatgg caatgatgta aaatgggtcg
      361 atgaattagg tgctaaatgg gctggttatt ttagggaaag aatgaaagat tatacaaatt
      421 atcgcggtgg tctatatcat accggaatgt atacagtaag tgctcatgtt ccaatgggtg
      481 aaaatgtagg tgcatcacct gatggtagaa atgctttgac accattggct gatggtggaa
      541 tgtcaccagt ttatggaaga gatatggcag gaccaactgc agttttaaaa tcggtatcac
      601 gtatgaagga ttcgtataca acaaatgggg gcttgttaaa tatgaaattt ttaccagagt
      661 tttttaaaac tgaaacagga atgatgaaat ttgaaaactt tttaagagca tttgtagatt
      721 taaaaattcc acatattcaa ttcaatgtag tacgacgtga agatttgttg gatgcgaagc
      781 ttcatccgga acaacatagg tcattaacga ttcgggttgc aggatataca gcttattttg
      841 tagaactggc tggtaagctg caagatgaga ttattgaaag gacggcttat gaagatatct
      901 aaagctttag tatttgatat caaaagattt gctgttcatg acggatatgg attaagaaca
      961 acagtttttt ttaaaggctg tccattaagg tgcaaatggt gtcaaaatcc agaaggatta
     1021 tcatctcaaa gaagaccaat atattttgaa aatagttgta ttcattgtca aagatgtgtg
     1081 gagttttcta aaaaaaatca aattaaatat gaaaataatc gtccgtattt caatttgcaa
     1141 tatgaaggaa cttttgataa tttggtaaaa gcttgtccag gtaatgctat tcgttatgat
     1201 agtgaagcgt atgatataaa acaattaatg gaaaaaatca aggaagatag agtgtttttt
     1261 agagatgatg gtggtgtgac attttctgga ggggaaccgc ttatgcaagg agaattcttg
     1321 tatgagattt taaaagcttg tcaagaagag aaaattcata ctgcgattga aacaactatg
     1381 tatggatcat tagaattaat caaaaaaata ctcccatatc ttgatttaat atatattgat
     1441 ttaaaagttt ttgatgaaaa gagacacatg gaattaacta acgtttcatc aaaaatgata
     1501 aaacagcata ttgaatatat tttagaaagt gaatatagaa acaaagttat aatcagaaca
     1561 ccattgatac cgacaatgac ggcgactgat cataatataa aaagtattgc aaattttctt
     1621 gttaatattt atccagaagt taaatacgaa ttgttgaatt acaatccatt agcttttgca
     1681 aaatatgaat tagttgatct ggaatatgaa gttgataaac aattgaagat gtttgataaa
     1741 gaacagatgg aacattttca tcagttagtt tatcaaacag gtttaaaaaa tttaatcata
     1801 gaataaggag aagataaaaa tggaatttat tattgatact gtgaatttag aggaaattaa
     1861 agatgcta
//
LOCUS       DAOMTA010000669         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3139_length_1866_cov_2.181115, whole genome shotgun sequence.
ACCESSION   DAOMTA010000669 DAOMTA010000000
VERSION     DAOMTA010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_1866_cov_2.181115"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLK68_14305"
     CDS             complement(<1..715)
                     /locus_tag="PLK68_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRM91620.1"
                     /translation="MGDISNGTRILLTQYLLKRLTDEDHALSRDELIKRIGGLGTTIS
                     SNTIKADIESIKSYNNLIKDCQDELLTPFICNESGSINYATKQKKGAYVEKTYYTDAQ
                     LILLTRLLGRGLHLESQNDSKLLEKVLSDSSLYKINHYHLLDNINSTYEDNCITVYLE
                     QIIKAINTFACVSFKELEYSVVENKVIDRPSHKTILLYPTNIDIKNDQVYLVGYLINS
                     SEDFELAYYRIDSIDQLKIT"
     gene            complement(895..>1866)
                     /locus_tag="PLK68_14310"
     CDS             complement(895..>1866)
                     /locus_tag="PLK68_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM91621.1"
                     /translation="VLVMGKKTKIALTGSLTMLAAGIGSLFYAGNYLYNLALNKQANK
                     DAIFTNPSTNNQIPDKDRTDINKKGDSSNFFDTIHYQDVFLDSDDGLKLHAYQFLNYG
                     HDYVIIVHGYTSEGKLMHASAKHFYEQGYNLLLPDLRGHGQSEGDYIAMGWLDRLDII
                     NWIKYLIDNDSKVKIILYGVSMGAATVMNVTGEKLPVNVIAAIEDCGFTSTWEMFSYQ
                     LKEMYNLPSRPFLDIANIVTQIRAGYSFGKAEAIEQVKKSTTPTLFIHGDKDRFVPFK
                     MLNQLYQSADCPKEKLVIKGAGHAQCEKIGGQVYWSKIDSFIRKYQD"
BASE COUNT          635 a          339 c          212 g          680 t
ORIGIN      
        1 gagttatttt caattgatca atgctatcaa ttcgataata tgccaattca aaatcttcac
       61 tactatttat taggtaacca actaaatata cttgatcatt ttttatatca atattagttg
      121 gatataataa aattgtttta tgactaggcc tatctataac tttgttttca acaacactat
      181 attccaattc tttaaatgat acacaagcaa atgtatttat agccttaata atttgctcta
      241 aatacactgt aatacaatta tcttcataag ttgaattgat attatctaac aaatgataat
      301 gatttatttt atacaaagaa ctatctgata atactttttc taataatttt gaatcatttt
      361 gactttctaa atgtaatcct cgacctaata accttgtcaa taaaattaat tgagcatcag
      421 tataatatgt cttttcaacg tacgcacctt ttttttgttt tgttgcataa ttaatactac
      481 cactttcatt gcatataaaa ggggtaagca actcatcttg acaatctttt atcaaattat
      541 tataagattt tattgattca atatctgctt taatcgtatt gctagaaatc gtcgttccta
      601 acccaccaat tcttttaatt agttcatcac gtgataaagc atgatcttcg tctgttaatc
      661 tttttaataa atattgcgtt agcaatatgc gagttccatt actaatatct cccattcctc
      721 aaccctccat actctatatt ttactttatc ttccaaaata tgtaaaatta gtaaatataa
      781 taaaaaccaa ttatttggtg tataaaattg tatacataaa attaatttca ctatccagca
      841 aaaaagtata tgtttatgac aaatttatga caataaaaaa gactaattta ttaattagtc
      901 ttgatatttt ctaataaaac tatcaatttt actccaataa acttgaccac caattttttc
      961 acattgagca tgtccggcac ccttgattac caatttttcc ttaggacaat cagcactttg
     1021 atataattga ttgagcattt taaaagggac aaaacgatcc ttatcgccat gaataaataa
     1081 tgttggcgta gttgattttt tcacttgttc gattgcttca gctttgccaa atgaataacc
     1141 ggctcgaatc tgagtcacaa tatttgcaat atctaaaaat ggtcgacttg gcaaattata
     1201 catttctttt agctgatagg aaaacatctc ccatgtacta gtgaatccac aatcttcaat
     1261 agcagcaata acattgacag gcaatttttc ccctgttaca ttcattactg tagccgctcc
     1321 catagatact ccatacagga taattttaac ttttgaatca ttatcaatta aatatttgat
     1381 ccagttaatt atgtctaaac gatcgagcca gcccattgca atatagtcac cttcactctg
     1441 cccatgacct cgaagatcag gaagcaaaag attgtaacct tgctcataaa aatgtttagc
     1501 actcgcatgc atcaatttac cttcagatgt atatccatgg acaataatca cataatcatg
     1561 cccgtaattc aaaaattgat aagcatgcag ttttaatcca tcatcactgt ctaagaatac
     1621 atcctgataa tgaatagtat caaaaaaatt gctactatct cccttcttat ttatatcggt
     1681 tctatcttta tcgggtattt gattatttgt tgagggatta gtaaatatcg catctttatt
     1741 agcctgttta tttaatgcca aattatataa ataattacca gcataaaata aactaccaat
     1801 tccagctgcc aacatagtta aactaccagt taatgcaatt ttcgtctttt ttcccattac
     1861 tagaac
//
LOCUS       DAOMTA010000670         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3141_length_1865_cov_2.608840, whole genome shotgun sequence.
ACCESSION   DAOMTA010000670 DAOMTA010000000
VERSION     DAOMTA010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3141_length_1865_cov_2.608840"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..919
                     /locus_tag="PLK68_14315"
     CDS             98..919
                     /locus_tag="PLK68_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM91622.1"
                     /translation="MVETRTIVYDEQLQIEAYQFVGIMQKFPNHFHDCYVVGFVEKGN
                     RHLSCRGDEYDMEPGDMLLLNPRDNHTCESRDGQPLDYRCLNIDADVMVRAVKEVIGC
                     SYLPNFEKPVAFQSEMVESLRTLRQSVMKKETEFLKEELFYILIEQLVKEYTNNQPLL
                     KSRFSEPIKLVKKHLDDNFINQISLDSLSELAQMNKYTLIRNFARSFGITPYQYLETI
                     RVNHAKELLEQGLSPLEAAMLSGFSDRSHFTRFFKSLIGLTPKQYQNIFKGKEDE"
     gene            912..1802
                     /locus_tag="PLK68_14320"
     CDS             912..1802
                     /locus_tag="PLK68_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM91623.1"
                     /translation="MNKDFSGHLAALLTIFIWGTTFISTKILLTDFKPIEILFFRFLI
                     GLFILILIYPKRLRKTTKKQELTFALTGLCGVTLYYLLENIALTYSMASNIGVIISIA
                     PFFTAILSHFILKEETLNQNFIIGFMAAMVGIMLISFNGSSNFKLNPLGDILALLAAL
                     VWAVYSILTKRISDYGYSTIQVTRRTFMYGVTFMFLTLIPLGFKLDLGRFSNPIYLGN
                     ILFLGLGASALCFVTWNMAVKILGAVKTSIYIYIVPVVTVVTSIIVLHEQITLMAFIG
                     TVLTLIGLFLSQKREVQKNG"
BASE COUNT          601 a          243 c          362 g          659 t
ORIGIN      
        1 tgatgcaggg agtctagctc ccttttttat ttatgtcaat atcattcaag caatatttta
       61 aaacttgtga tatagtagca ttcaaggagg gatacaggtg gtggaaacta gaacgatcgt
      121 atatgatgaa caattacaaa ttgaagcata tcagtttgtt ggaataatgc aaaaattccc
      181 taatcatttt catgactgtt atgtagttgg ttttgttgaa aagggcaatc gccatttgtc
      241 ttgtcgtggg gatgaatatg atatggagcc aggggatatg ctgttattaa atcctcgtga
      301 taatcacact tgtgaaagtc gcgatggtca accgcttgat tatcgttgtt taaatattga
      361 tgcagatgtt atggtgcgag cagtaaaaga agttatagga tgtagctatt taccaaattt
      421 tgaaaaacca gttgcttttc aaagtgagat ggtcgaatct ttacgcacat tgcgtcaaag
      481 tgtgatgaag aaagaaacgg agtttttgaa agaagaatta ttttatattt tgatagaaca
      541 gctggttaaa gaatatacta ataaccagcc gctattaaaa agtcgattta gtgagccgat
      601 caaattagta aaaaagcatt tagatgataa ttttatcaat caaatttctt tagattcatt
      661 aagtgaatta gcacagatga ataagtatac tctaattcgt aattttgctc gttcttttgg
      721 gataactccc tatcaatatc ttgaaacaat acgtgttaat catgccaaag agctacttga
      781 acagggtctc agtccacttg aggcagcaat gctttcggga tttagtgatc gaagtcattt
      841 tactcgtttt tttaagagct taatcggctt gacaccgaaa cagtatcaaa atatttttaa
      901 ggggaaagag gatgaataag gatttttcag gacatttggc tgcattatta acaattttta
      961 tttggggaac aacatttatt tcaactaaaa ttttattaac tgattttaaa ccgatagaaa
     1021 tattattttt tcgtttcttg ataggtttat ttattttaat tttgatttat cccaaaagac
     1081 taagaaaaac gacgaagaag caagagttga catttgctct aacgggactt tgtggagtta
     1141 ctttgtatta tttattagaa aatattgcac taacatattc gatggcttca aatatcggag
     1201 tgataatctc aattgcaccg ttttttacgg caatcttatc acactttatt ttgaaagaag
     1261 agacattgaa tcaaaatttt ataattggtt ttatggctgc aatggtgggg attatgctta
     1321 ttagttttaa tggcagcagt aattttaagc tgaatccatt gggagatata ttagcattac
     1381 tggctgcttt ggtctgggct gtttattcaa ttttaactaa acgaattagt gattatggtt
     1441 atagtacgat tcaggtcaca agaagaactt ttatgtatgg ggtaacattt atgtttttga
     1501 cattgatacc tttaggattt aaattggatt taggacgttt tagtaatcca atttatttag
     1561 gaaatatctt atttttagga ttaggtgcat ccgctttatg ttttgtaact tggaatatgg
     1621 cagtaaaaat attaggtgct gtaaagacga gtatctacat atacattgta ccagtagtta
     1681 cagtagtgac ttcaatcatt gttttacatg aacaaattac tttaatggct ttcatcggga
     1741 cagtactgac tttgattgga ttatttttat ctcaaaaacg cgaggtacaa aagaatggat
     1801 agaaaatgtg ttatgatagt tgataaaaat ttgccactag gattaattgc taatacaact
     1861 gctat
//
LOCUS       DAOMTA010000671         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3149_length_1863_cov_2.439159, whole genome shotgun sequence.
ACCESSION   DAOMTA010000671 DAOMTA010000000
VERSION     DAOMTA010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3149_length_1863_cov_2.439159"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..320
                     /locus_tag="PLK68_14325"
     CDS             51..320
                     /locus_tag="PLK68_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRM91624.1"
                     /translation="MKYEFETRCIHPANLELKQHPYGAVSIPIYQTAIFSHPGIGESR
                     GYNYSRESNPTRAYLEEIISNLEGAVDTVACSSGMAAISLVLELF"
     gene            334..1209
                     /locus_tag="PLK68_14330"
     CDS             334..1209
                     /locus_tag="PLK68_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM91625.1"
                     /translation="MICSNDLYGGSTRLFTLQQNKGLKFSYVDTTNLKSFKQYICPQT
                     KAVFIETPSNPTMQISDLKKISALCQAHKLLLIVDNTFLSPYFQNPLLLGADIVIHSA
                     TKFLSGHNDIIAGSISTGDHKLAKRIREVSKTVGNSLSPLDCYLLIRGIKTLAIRQKS
                     QQENALKISNWLNRHPRVSKVYYPGLIEHPGYEINRNQARGFGSMIAFSVENHFLAKK
                     ILENVQLITYAESLGGVESLITYPMIQTHGDVPAEIRAKLGITDNFLRLSVGIENIDD
                     LLADLRQAFGDNDKI"
     gene            1196..>1863
                     /locus_tag="PLK68_14335"
     CDS             1196..>1863
                     /locus_tag="PLK68_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM91626.1"
                     /translation="MIKSNFDSIINRTDTNSLKYDFTSERERPDDILPLWVADMDFIV
                     APEIQQRLYDVVNHGIYGYSDSKDGYYQAIYSWYRSKFNYEIKKEWIVKTPGVVFALA
                     QAVRAFTKLGDGVIIQTPVYYPFKEVIIDNGRRVITNSLVLKNAHYEIDFDDFEEKIK
                     KEKVKLFILCSPHNPVGRVWKKDELLKLGQICLKNDVKIISDEIHSDFIYPGHQHHVF
                     ASLKT"
BASE COUNT          637 a          251 c          365 g          610 t
ORIGIN      
        1 tcaagaaata tttgtgtcca tgcgcttagc gtggggaaat ggagaaaagt atgaaatatg
       61 aatttgaaac tcgttgtatt catcctgcaa atttagaatt gaaacagcat ccttatggtg
      121 ctgtttcaat tccaatatac caaacagcaa ttttttctca tcctgggatt ggggaaagca
      181 gagggtataa ctactctcgt gaaagtaatc caacaagagc ttatttagaa gagattattt
      241 ccaatttaga gggcgcagtt gatacagttg cttgttctag tggaatggca gcaatttcat
      301 tggtactaga acttttttag tagtggtgat catttgatat gttcaaatga tttatatggc
      361 ggttcaactc gactatttac tttgcagcaa aataaaggtt taaaattcag ttatgttgat
      421 acaactaatt taaagagttt taagcagtat atttgtcctc aaacaaaagc tgtttttatt
      481 gaaacgccga gtaacccaac aatgcaaata agtgatttaa aaaaaattag tgccttgtgt
      541 caagcccaca agctgttact aattgttgat aataccttcc tttcaccata ttttcaaaat
      601 ccattattat taggtgctga tattgtaatt catagtgcta ctaagtttct aagtgggcat
      661 aatgatatta ttgctggaag tatttcgact ggtgatcata aactagctaa aaggattaga
      721 gaagtttcaa agacagttgg aaatagcttg tcaccactag attgttattt attgattcgg
      781 gggatcaaga cgctggcaat cagacaaaaa tcacaacaag aaaatgcgct gaaaatttca
      841 aactggttaa atcggcatcc gcgggttagc aaagtttatt atcctggttt aatagagcat
      901 ccaggatatg aaatcaatag aaatcaagcc cgcggctttg gcagcatgat agcctttagt
      961 gttgaaaatc attttttagc aaaaaagatt ttagaaaatg tacaattaat tacttatgca
     1021 gaaagtcttg gaggtgtgga gtcattgatt acttatccaa tgattcaaac ccatggtgat
     1081 gttccagcag aaattagagc taaattagga attacagata attttttgcg attatcagta
     1141 ggaatagaaa atattgatga tttattagct gatttgaggc aggcttttgg tgataatgat
     1201 aaaatctaat tttgattcaa taataaaccg tactgataca aactccttaa agtatgattt
     1261 cactagtgaa agagaacgtc ctgatgatat tttaccgtta tgggtggcag atatggattt
     1321 tatcgttgct ccagaaatac aacaacgatt atatgatgtg gtaaatcatg gtatttatgg
     1381 atatagtgat agtaaggatg gttattatca agctatttat agttggtatc ggtcaaaatt
     1441 taattacgaa ataaaaaagg aatggatagt taagacgcct ggtgttgtat ttgctttagc
     1501 acaagcagtg cgggcattta ctaaattggg tgatggagta attattcaaa caccagtata
     1561 ttatcctttt aaagaagtaa ttattgataa cggaagacgt gttataacaa attctttagt
     1621 tttaaaaaac gctcattatg aaatagactt tgatgatttt gaagagaaga ttaagaaaga
     1681 aaaagtaaaa ttgtttattt tatgttcgcc acataatcca gttggtcgag tttggaaaaa
     1741 agatgagcta ttaaaattag ggcaaatctg ccttaaaaat gatgttaaaa ttattagtga
     1801 tgaaatccat agtgatttta tttatccggg gcatcagcat catgtttttg catcattaaa
     1861 gac
//
LOCUS       DAOMTA010000672         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3159_length_1859_cov_2.644678, whole genome shotgun sequence.
ACCESSION   DAOMTA010000672 DAOMTA010000000
VERSION     DAOMTA010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3159_length_1859_cov_2.644678"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLK68_14340"
     CDS             <1..776
                     /locus_tag="PLK68_14340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRM91627.1"
                     /translation="RKADRPNVREYIDNLFDDFIELHGDRLYKDDGSIVGGLAMFNNI
                     PCTVIGHLKGRTLEENLHCNFGMSSPEGYRKAMRLMKQAEKFNRPIVTFVDTPGAYPG
                     LKAEKHGIGEAIARNLMEMSQLTVPIIVIVIGEGGSGGALALSVGDRMVMLENSVYSV
                     LSPEGFASILWKDKDGSRVHEAAELMKLTANDLYEMKIIDKIIKEPRGGITKNREYVY
                     KRLRIYLRNTLEELMKLSKTSLVNKRYNKFREMGRITND"
     gene            769..1230
                     /locus_tag="PLK68_14345"
     CDS             769..1230
                     /locus_tag="PLK68_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM91628.1"
                     /translation="MTNRDILNRELINQLFVRLFNQILDIETQYMVGHGVEDLSLSEL
                     HIIDAISSLSNPTMSTIASQATLTNGTITTAIKKLEAKGYVKRRKDDNDRRIIRVELT
                     AKGNRVCKVHRDFHEEMVSRVCEDSHVLDDELLIKSLQQLLYFFEDIKEKY"
     gene            1239..>1859
                     /locus_tag="PLK68_14350"
     CDS             1239..>1859
                     /locus_tag="PLK68_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRM91629.1"
                     /translation="MSGLKILSTGYYAPQKVLDNFDLEKVVETSDEWIVSRTGIKRRH
                     IAENESCVELGYQAALKAVEKIDKSKIGLIICATMTPDYFTPSTACLIQERLGLNDQE
                     VMCFDLNAACSGFVYALTVAQALLQNMEEKYALVIGSEEISKIMDFKDRNTCVLFGDG
                     AGALVVGNGDGIFASYSNSAGNLEALKAPAISKSNQDHYLTMAGQEV"
BASE COUNT          635 a          258 c          408 g          558 t
ORIGIN      
        1 ctcgtaaagc tgaccgtcct aatgtccgtg aatatattga taatttattt gatgatttta
       61 ttgaattaca tggggaccgt ctttacaaag atgatggttc aattgtcggg ggattagcaa
      121 tgtttaataa tattccctgt actgtaattg gacatttaaa aggacgaact ttagaggaaa
      181 atctacactg taattttgga atgtcttcac cagaagggta tcgtaaagcg atgcgtttga
      241 tgaaacaggc ggaaaaattt aatcgaccaa ttgttacatt tgtagataca ccaggtgctt
      301 atccgggttt aaaagctgaa aaacatggaa tcggtgaagc aattgcgcgt aatctaatgg
      361 aaatgtcaca gctgacggtg cctattattg tgattgtaat cggtgaaggc ggcagtggtg
      421 gagctttggc actaagtgtt ggagatcgta tggtaatgct tgaaaatagt gtttattcgg
      481 ttttatcacc agaaggcttt gcatcaatct tatggaaaga taaagatggt tcaagagttc
      541 atgaagctgc agagctaatg aaattgactg caaatgattt atatgaaatg aagatcattg
      601 ataagatcat caaagaacct cgaggtggaa ttacaaagaa ccgcgaatat gtatacaaac
      661 gtttaagaat ctatcttcga aatactcttg aagaattgat gaaattatca aaaacatcat
      721 tagtaaataa acgttacaat aaatttagag aaatgggtcg aattacgaat gactaatcgc
      781 gatattttaa atcgagaatt aattaatcaa ctgtttgtaa gactgtttaa tcaaattttg
      841 gatattgaaa cccaatatat ggtaggacat ggagtggaag atttaagttt atcagaactg
      901 catattatcg atgcaatcag ttcgctttca aatcccacga tgtcaacaat tgcttctcag
      961 gcgactttaa ctaatggaac aattacgaca gcaataaaaa agctcgaagc taagggatac
     1021 gtaaaacgtc gtaaagatga taatgatcga cgcattattc gtgtagagct gacagctaaa
     1081 ggcaatcgtg tttgcaaagt acatcgagac tttcacgaag agatggtaag tcgtgtctgt
     1141 gaagatagtc atgttcttga tgatgagtta ttgattaaat cattacaaca gttattgtat
     1201 ttctttgaag atatcaaaga aaaatattag gagattatat gagtggatta aaaatattat
     1261 caactggtta ttatgcacca caaaaagtac ttgataattt cgaccttgaa aaagtggttg
     1321 aaacatcaga tgagtggatc gtctctagaa ccgggataaa aagaagacat attgctgaaa
     1381 atgaaagttg cgtagaactt gggtatcaag cagctttaaa ggcagttgaa aaaatagata
     1441 aaagtaagat tggattgatt atctgtgcaa caatgacacc cgattatttt actcccagca
     1501 ctgcttgttt gattcaagaa agattaggtt tgaatgatca agaagtaatg tgctttgatt
     1561 taaatgctgc ttgtagcggc tttgtgtatg ccttaacggt agcccaggca ttattacaaa
     1621 acatggaaga gaaatatgct ttagtcattg gaagtgaaga aatttccaaa ataatggatt
     1681 tcaaagatcg caatacttgt gttttgtttg gtgatggggc tggagccctt gtagttggaa
     1741 atggtgatgg tatttttgct agctacagta attcagcggg aaatttagaa gcattaaagg
     1801 ccccggcaat cagtaaatct aatcaggatc attatttaac gatggctggg caggaagtg
//
LOCUS       DAOMTA010000673         1858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3163_length_1858_cov_2.139767, whole genome shotgun sequence.
ACCESSION   DAOMTA010000673 DAOMTA010000000
VERSION     DAOMTA010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3163_length_1858_cov_2.139767"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>635)
                     /locus_tag="PLK68_14355"
     CDS             complement(<1..>635)
                     /locus_tag="PLK68_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91630.1"
                     /translation="PYINDPVFLEKLDKFIKAFGAEFNNDLDIAFIDGMGFGNWGEAH
                     HVKFSTEWDDNVYGAVEKVIKIYDKYFPDVLLGAQEGQPENYGTQENDEIINTDKPYT
                     GAFDKEYDFVVRRDTFGWMTDAIRNQTLEWFNQGIPVFAENCYHSFKVREYWYNNAGY
                     PTLDGILRQVVADALTCRANTLDARVVMDCQKWLENDQQNGSGLLNKFGLNG"
     assembly_gap    636..673
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<674..>1858)
                     /locus_tag="PLK68_14360"
     CDS             complement(<674..>1858)
                     /locus_tag="PLK68_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91631.1"
                     /translation="GYLSLGNIFILELQSNENIVINEIETEGVYLKDNLLSNARVYHN
                     GENIDAAGMVDNNVSSYYKTTYSDSDEYILDFSPRTITTNELVYTAHFPNSQGIGNLT
                     VEIWDGNSWKNVDTFDFKHKTDNQIRESQQLIFNELTTSKIRLIVNKANLVWDNSYLF
                     TDLNAIGTINNNAQYYAESIDTNIVVNQGESKFPIPHLKGYEVSINSSDHEEIIDLNG
                     NINAPLTDTKVKITLKVSSEDGQDVGITDEFEVVVPRRVLPGNKLISTIVDNETIYNN
                     PSMGWVQYYEFQDTDVDKYWAEMDELYAQGLKTNILYIRNPWSWYEPSEGNYAWNDPD
                     SRLSKLISGARARGIQLAFRVLVDSSDAFQQATPEYVFKAGASWYKTDRTDDTAPEID
                     AKD"
BASE COUNT          596 a          373 c          213 g          638 t
ORIGIN      
        1 ccattcaaac caaatttatt caataaacca gaaccgtttt gttgatcatt ctctagccat
       61 ttctgacagt ccattacaac ccgagcatct agagtatttg ctcgacatgt caaagcatca
      121 gctacgacct gacgtaaaat accatccaaa gtcggataac cagcattatt ataccagtat
      181 tctcgtactt taaatgaatg ataacaattt tctgcaaaaa ctggtattcc ttgattaaac
      241 cattctaatg tttgatttct aattgcatcc gtcatccagc caaatgtatc acggcgaaca
      301 acaaaatcat attctttgtc aaatgctcca gtataaggtt tgtcagtatt aatgatctca
      361 tcattttctt gagtcccata attttctggt tgtccttctt gagctcccaa taaaacatcc
      421 gggaaatatt tatcatatat tttaatcact ttttcaaccg caccataaac attatcatcc
      481 cactcagtac taaatttaac gtgatgtgct tcaccccagt taccaaagcc cataccatca
      541 ataaaagcaa tatctaaatc attattaaac tctgcaccaa atgctttaat aaacttatct
      601 aatttttcta agaaaacagg atcattaata tatggnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnatcttta gcatcgattt ctggtgccgt atcatcagtt cgatcagttt
      721 tataccagct tgctcctgct ttaaatacat attctggtgt tgcctgctga aaagcatcac
      781 ttgaatccac caatacccta aaagctaatt gaattcctcg cgctcttgct ccactaatta
      841 atttactcaa cctgctatca ggatcattcc atgcataatt tccttctgat ggttcatacc
      901 agctccaagg attacgaata tataaaatat ttgtctttaa accttgagca tataattcat
      961 ccatttcagc ccaatattta tcaacatcgg tatcttggaa ttcataatat tgaacccatc
     1021 ccattgacgg attattataa atcgtttcat tatcaacaat agttgaaatc aatttattgc
     1081 cgggcaaaac tcttcttggt acaacaactt caaactcatc tgtgatacca acatcctgac
     1141 catcttcact actaaccttt aatgttattt taacttttgt atctgtcaaa ggtgcattaa
     1201 tattaccatt taaatcaatg atttcctcat gatcagaact attgatcgat acctcatagc
     1261 cttttaaatg aggaattgga aatttgcttt caccttgatt tacaactata ttggtatcaa
     1321 tactttcagc atagtactga gcattattat ttattgttcc aattgcattt aaatctgtaa
     1381 ataaatagga attatcccaa actagattag ctttatttac gatcaagcga attttactag
     1441 ttgtaagttc attaaaaata agctgctgtg attctctaat ttgattatcg gttttatgtt
     1501 taaaatcaaa tgtatcaaca tttttccaac tattaccatc ccaaatttcc acagtcaaat
     1561 taccaatacc ttgtgaatta ggaaaatgtg ccgtataaac aagttcatta gttgtaattg
     1621 tccgtggtga aaaatctaag atatactcat cactatcaga atatgtagtt ttataataac
     1681 tacttacatt attatcaacc attccagctg catcaatatt ttcaccatta tgataaactc
     1741 gggcatttga taaaagatta tcttttaaat atacaccttc tgtttcaatt tcattgatta
     1801 caatattttc attactttgt aattctaaaa taaatatatt tcccaaagat aaataacc
//
LOCUS       DAOMTA010000674         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3166_length_1857_cov_2.219201, whole genome shotgun sequence.
ACCESSION   DAOMTA010000674 DAOMTA010000000
VERSION     DAOMTA010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3166_length_1857_cov_2.219201"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLK68_14365"
     CDS             <1..480
                     /locus_tag="PLK68_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM91632.1"
                     /translation="LGGAFALDFAIRNPYFIERLILIETMIYLPKWLWLTLLPGYCSG
                     YRIFQKQIKLFKILECCTKFKNISSSERIRISSREWNRTVNSFYLKLMNEYEKQNHIK
                     RCQQINCPVNIIYSQASFKNIRKTAQILSQFSFVELHLCQGRGHFLFLDETMNRIVI"
     gene            794..1105
                     /locus_tag="PLK68_14370"
     CDS             794..1105
                     /locus_tag="PLK68_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91633.1"
                     /translation="MWGGVEQFISNYYHHSDFTLEAFRDLELTISKDYLQAAINIYPN
                     KRIEMYSNQEISNQLDQIDEQYYADHEIEYFDFIDKYTEGNIVKMDEIMFKIGAFSKL
                     T"
     gene            1671..>1857
                     /locus_tag="PLK68_14375"
     CDS             1671..>1857
                     /locus_tag="PLK68_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91634.1"
                     /translation="MACMMIYGDFSNIADTYLSLVNWLQKMLSIRFQDPIALSVVEVI
                     VIDYQTEIVSIPYIDINP"
BASE COUNT          680 a          204 c          331 g          642 t
ORIGIN      
        1 ttagggggtg catttgcact tgattttgcg attcgtaatc catattttat tgagcgttta
       61 attttgatag aaacaatgat ttatttaccg aaatggttat ggttgacact gttgccagga
      121 tactgcagcg gctatcgaat atttcaaaag cagattaagt tatttaaaat attagaatgt
      181 tgtacgaaat tcaaaaacat tagttcatca gaacgaataa gaatatcgtc aagggaatgg
      241 aatcgtactg ttaattcatt ttatttaaag cttatgaatg agtatgaaaa acaaaatcat
      301 attaagcgat gtcaacaaat caactgcccg gttaatataa tttatagtca agccagtttt
      361 aaaaatattc gaaaaactgc gcaaatctta tcacagtttt catttgtaga actccatttg
      421 tgtcaaggca gaggtcattt tttgtttctt gatgagacta tgaatagaat agtgatataa
      481 aggctggaag ggaagaaata atgcagaaga aaacaattat tatgggaata gtcatatgtc
      541 tattagggat tggttatttt ttattaaata agccttgatt atctgatgaa gatggagaat
      601 tatttaatta tctacttaat ggttataatc ctgatttgtc aatcacagaa attatagttc
      661 aagaggggga agaaagatta tttaatctct ttacattaat tgacgaaaaa tctaattatg
      721 gtgaacggtt taatgattgt actctggtag agcaaaattt ttggtttctc tataattttc
      781 atatggaagt tgaatgtggg ggggagttga gcagtttatt tcaaattatt atcatcattc
      841 tgattttacg cttgaagctt ttagagattt ggaactaacg ataagtaaag attatttaca
      901 agctgcaatt aatatctatc caaataaacg aatcgagatg tactcaaatc aagaaatcag
      961 taatcaatta gatcaaattg atgaacagta ttatgctgat catgaaatag aatattttga
     1021 ttttattgat aaatacacag aaggaaatat tgtaaagatg gatgaaatca tgtttaaaat
     1081 tggagcgttt tctaaattaa cataggctac tgttagaatg ttaagatatt atgatgaaat
     1141 gggactttta aaactggctg cagttgatta aacaactgga tatcgcgcca tgtattcaag
     1201 tgaacaaatt cttcgcttaa ataaaattat atatttgtaa ggtagtggat ttaattaaca
     1261 gagatagcac ttgttttaaa taaccaagga aatcagttta ttattgaata actggagcaa
     1321 aaatatattg gaggaacaag taaaattaaa aaaatcgaat tagcaaaaaa agaattatta
     1381 ggtaataaaa atgggatgaa ttataatgtt ctaataaaat aaactcctag ttattttgta
     1441 ctttcattta gaaaaataat ttctgattat tatgctgaaa gtaagttgta ggaagaatta
     1501 ttaaacttta ctgctgagca tagaagagat gtttcagcaa atatttttca atttattatg
     1561 atgaagatta ccgtgaatat gatgttgatg tagaattgtg agtatcaatt agaaaagctg
     1621 tcactatcga gaagcttttt aaatttcgat acacctcagc tgttgaaata atggcttgta
     1681 tgatgatata tggagatttt tctaatattg ctgatactta tttatcatta gtaaattggt
     1741 tacaaaaaat gctcagtata agatttcaag atccaattgc tttaagtgtt gtagaagtca
     1801 tcgtaatcga ttatcaaaca gagatagtta gtatccctta tattgatatc aatccat
//
LOCUS       DAOMTA010000675         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3169_length_1856_cov_2.011105, whole genome shotgun sequence.
ACCESSION   DAOMTA010000675 DAOMTA010000000
VERSION     DAOMTA010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3169_length_1856_cov_2.011105"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLK68_14380"
     CDS             complement(<1..763)
                     /locus_tag="PLK68_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91635.1"
                     /translation="MSKKLDELFETYAYDPRQKTQLRLADEKNLDISKMKDPRFNWEQ
                     MREISLAMEYGLKPDTLCDPEINAESMEKIRYSLMDQQSVFEDAKEEVKKKRTKRISL
                     IIFTVIVGFIVSIIYLMNKDTINKYIEPVPLELTTDRVTVEYGEDIHFMDYVKYYDKS
                     QQLTIPLNQKLKKIKDYKFIYLVTNGVKTKEKTLIVSVVDKTKPTIELTQSEITLDSG
                     EQFTPKDYIKKVEDNYDQLSSDNVVIKNTVDTNKDG"
     gene            complement(764..>1856)
                     /locus_tag="PLK68_14385"
     CDS             complement(764..>1856)
                     /locus_tag="PLK68_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRM91636.1"
                     /translation="IDEKYIYQNTCFENKLIKYTMLGKKKELFLRNCKIEATSLSSLD
                     IKERIVFHNCIFDNVKISKCNFKNAVFYNCRFNNSVIVNSILANSYFINCKGIETVDF
                     MENNMQDVSVIGSKVNLNELVEKQNRFEDILIQEEMLEVQHGNVAVLEEKKEDDQEMK
                     EENERQIIKNEIYSSQELSQDFLNNKLFYKCHFVDCSVSDNVKLNATTDFKECTFLNN
                     DLHFDDVDKTSFDDCIFEKVSLKGNFIHASFAGSKFSNLSFIRDSTFVACLFDHTNAI
                     DYFDNNLIKLSNIELVKKSNDNSEIMKKVNELYEILKSEDKVLQQALIAKTKVDEEDV
                     VDYILSLDDIEYLKLIAKTSNRVVEEKMV"
BASE COUNT          586 a          320 c          197 g          753 t
ORIGIN      
        1 caccatcttt atttgtatca acagtatttt taattacaac attatcagat gataactgat
       61 cataattatc ctctactttt ttaatataat cttttggagt aaactgttcc ccactatcaa
      121 gagtaatttc actttgtgtc aattcaattg ttggttttgt tttatcaacg acactaacta
      181 tcagtgtttt ttcttttgtt ttaacaccat tcgtaactaa ataaataaat ttgtaatcct
      241 ttatcttttt taacttttga ttcaaaggta tcgttaattg ttgagatttg tcataatact
      301 ttacataatc catgaaatga atatcttcac catattcaac agttactcta tctgttgtta
      361 attctagagg aacaggctcg atatacttat tgattgtatc cttattcatc aaatagataa
      421 tacttacaat aaaaccaact attacagtaa aaatgattaa agaaattctt ttagtacgtt
      481 tctttttaac ttcttctttt gcatcttcaa aaacactttg ctgatccatc agactatatc
      541 gaatcttctc catagattcc gcattgattt caggatcaca taaagtatca ggtttcaatc
      601 catattccat tgctaatgaa atttctcgca tctgttccca gttgaatctt ggatctttca
      661 ttttagaaat atccagattt ttttcatcag ccaaacggag ctgtgttttt tgccttggat
      721 cataggcata tgtttcaaat aattcatcta atttttttga catctatacc atcttttctt
      781 caactactcg attgcttgtt tttgcaatca gttttaagta ttctatgtca tcaagactta
      841 aaatataatc aactacatct tcctcatcaa ctttcgtttt tgcaatcagt gcctgctgta
      901 ataccttatc ttctgatttt aatatctcat aaagttcatt tacttttttc attatttcag
      961 aattatcgtt tgatttttta acaagttcaa tattgcttag ctttatcaga ttattatcaa
     1021 aataatcaat tgcgtttgta tgatcaaaca aacaagcaac gaatgtagaa tctctaataa
     1081 aagatagatt gctaaacttt gatccagcaa aggatgcatg tataaaattt cctttaagag
     1141 aaactttttc aaagatacag tcatcaaacg atgttttgtc aacatcatca aaatgaagat
     1201 cattgtttaa aaaagtacac tctttaaagt ctgttgtagc attcaatttt acattatcac
     1261 ttacagaaca gtctacaaaa tggcatttat aaaaaagctt attatttaaa aaatcttgtg
     1321 acaattcctg actgctatag atttcatttt ttataatttg tctttcattt tcttctttca
     1381 tttcctgatc atcttccttt ttttcttcca acacggcaac attgccatgt tgaacttcaa
     1441 gcatttcttc ttgaattaaa atatcttcaa atctgttttg tttttctacc agctcattta
     1501 aattaacttt gctaccaatt actgagacat cctgcatatt attttccata aaatcaactg
     1561 tctcaattcc tttacagtta ataaaataac tgtttgccag tattgaattt acaattactg
     1621 aattattaaa cctgcagtta taaaagactg catttttaaa attacatttg gaaattttaa
     1681 cattgtcaaa tatacagtta tgaaagacta ttctttcttt aatatctaaa cttgacagac
     1741 ttgttgcttc aatcttacag tttcttaaaa acagttcttt cttttttcca agcattgtat
     1801 atttgatgag tttattttca aaacatgtat tctgataaat atatttttca tcaatt
//
LOCUS       DAOMTA010000676         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3188_length_1849_cov_2.027313, whole genome shotgun sequence.
ACCESSION   DAOMTA010000676 DAOMTA010000000
VERSION     DAOMTA010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_1849_cov_2.027313"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLK68_14390"
     CDS             <1..824
                     /locus_tag="PLK68_14390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRM91637.1"
                     /translation="KQGNDATYFFKNVKNVFENDDLTIVNLEGPLTSATSHVEKQFPF
                     SGPKEYVNILTSGSVELVSIANNHSEDYYEQGMTDTKQVLDEKGIGYFGYETSCVKEI
                     KGIKIGFLGYRSMSLSMNNEKGRATIKAAINDLKNNQEANAVVVFYHWGIEREYYANS
                     DQRELAKFSIDSGADLVMGSHPHVVQGTEEYNGKQIVYSLGNFCFGGNRNPSDTDSMI
                     YSITMNFTDGVYTGDSHEIIPCSVTSVKGRNNYQPIILEGNEKERVLTKIQKYSY"
     gene            921..1205
                     /locus_tag="PLK68_14395"
     CDS             921..1205
                     /locus_tag="PLK68_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91638.1"
                     /translation="MKRKIVYFLYSGYIVFLVGLLGSRFANSISKGTLNIICLFIFIG
                     VLAFCCGGFVGYPTRQINQRVVYNDNSVINYFYYQLFLLSLKCDNFFVNA"
     regulatory      1233..1312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1503..>1849
                     /locus_tag="PLK68_14400"
     CDS             1503..>1849
                     /locus_tag="PLK68_14400"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRM91639.1"
                     /translation="MLTDVEIAQSAKMKPISEIAKKVGLEGDDLELYGKYKAKIALEA
                     INRLENNPDGKLILVTAINPTPAGEGKTTTMIGLSQALNKLGKKSVVAMREPSLGPCF
                     GIKGGAAGGGYAQV"
BASE COUNT          621 a          223 c          389 g          616 t
ORIGIN      
        1 ccaaacaggg aaatgatgct acatattttt ttaagaatgt caaaaatgtt ttcgaaaatg
       61 atgaccttac aattgttaat cttgaaggcc cattgacaag tgcgacttca catgtagaaa
      121 aacagtttcc ttttagcgga ccaaaagaat atgttaatat tttaactagc ggtagtgttg
      181 agctggtttc tattgccaat aatcattcag aagattatta tgaacaaggg atgacggata
      241 ctaagcaagt acttgatgaa aagggaattg gctattttgg ctatgaaacg tcatgtgtca
      301 aggaaattaa aggaattaaa attggctttt tggggtatcg ttcaatgtct ttatcaatga
      361 ataacgaaaa agggcgtgca acaattaaag cagcaatcaa tgacttgaaa aataaccagg
      421 aagctaatgc cgtagtcgtt ttttatcact ggggaattga gcgggaatac tatgcgaata
      481 gtgatcagcg tgagttagca aaattttcaa tagatagtgg tgcggattta gttatgggtt
      541 cacatccgca tgttgttcaa ggaacagaag aatataatgg aaaacaaatc gtttattcat
      601 taggaaactt ttgttttggt ggaaatcgca accctagtga tacagattca atgatttact
      661 caattacaat gaatttcact gatggtgttt atactggaga tagtcacgaa attattcctt
      721 gttcagttac ctcggttaaa ggacgaaata attatcagcc aataatttta gaaggtaatg
      781 aaaaagagag agtactaact aaaatacaaa aatatagtta ctagaagatg ggataaaacc
      841 atcttttatt ataaaataat atatataata tttgtaatct cattatatta tctttataat
      901 taacatagaa ggtgataatt atgaagcgca aaatagtata ttttctttat agtggttata
      961 ttgttttctt ggttggctta ttgggaagcc gttttgctaa tagtatcagt aaaggtactt
     1021 taaatattat ctgtttattt atttttattg gtgttttagc tttttgttgt ggtggttttg
     1081 ttggttatcc aactcgtcaa attaatcaaa gagttgttta taatgacaat tccgttatta
     1141 attattttta ttatcaatta tttttgttga gtttaaaatg cgacaatttt tttgtaaatg
     1201 cttgaatctt gaatcaataa atgatattat gcaaatagtt atacgactga cggaagtgga
     1261 attaaccaca gggagtataa tcatgtaaga gccgaccgtc tgggcaagag tctggacgag
     1321 tgtaggctct tttttaattc tttggtcaaa ttgcttgttt tgagggcgaa aagagtggtt
     1381 ttaaaacaac tgtactggtt gagaaaaaag ccgaaatatg atataaatta ggtgtatttt
     1441 tgttttgatt attggtgacc ttagaatgtt tatttaaaaa ttatatttga aagggtggcg
     1501 ttatgttaac tgatgttgaa attgcacaaa gtgcaaagat gaaaccaatt agtgagattg
     1561 ctaaaaaggt tggtttagaa ggtgatgatt tagaattata tggtaagtat aaagccaaga
     1621 ttgcacttga ggcgattaat cggctggaaa acaatcctga tggaaaattg atcttggtta
     1681 ctgcaattaa tcctactcca gctggggaag gtaagacgac aacgatgatc ggattgtctc
     1741 aagccttaaa taagttagga aaaaaatcag ttgtggcaat gagagaacca tctttagggc
     1801 catgttttgg gatcaaaggt ggagctgccg gtggaggata tgcacaagt
//
LOCUS       DAOMTA010000677         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3191_length_1848_cov_1.504183, whole genome shotgun sequence.
ACCESSION   DAOMTA010000677 DAOMTA010000000
VERSION     DAOMTA010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_1848_cov_1.504183"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLK68_14405"
     CDS             <1..652
                     /locus_tag="PLK68_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91640.1"
                     /translation="SNNFSSLGNIIYLQNKFILSELYRDFPHYKTIIGSNGNEKRLTT
                     PIFDQLDLFKENAIYDDSLCILGLIKNNRFYRYIPSKYIEQNENLNMYKVIIPKSNGT
                     GALGETLSSPLIGKPLIGYTQSFIGIGAFDNYDEAKAAKKYIETKFARAMLGILKVTQ
                     DNNRDVWEYVPLQDFTKKSDIDWNQTINEIDRQLYAKYNLTNDEIAFIESMIKPME"
     assembly_gap    799..808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            955..1593
                     /locus_tag="PLK68_14410"
     CDS             955..1593
                     /locus_tag="PLK68_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91641.1"
                     /translation="MTRLEKLYSIIENSKELGLQLGDDVLRQTSELEENIIKKEILPV
                     LTETIEPALSQVRRELVLVVDYVPDEPISVRISRKRNFSAEMEDSKEILPDPQVEHNI
                     GIIKRRGDKKAPRTNLRVKMDDGRIIEHRFALDTLKEVVQIVGIDKVRALNIIQCGVP
                     LISNTLDNVYSSSQKDLQNGWYLMTLSSTITKKQQLDKISDAYNLGMVVEIV"
BASE COUNT          711 a          257 c          320 g          550 t
ORIGIN      
        1 gtcaaataat ttttcttcat taggtaatat aatatacctt cagaataagt ttattctttc
       61 tgaattgtac agagattttc cacattacaa aacaataatt ggtagtaatg gtaatgaaaa
      121 aagattaact acgcctatat ttgaccaatt agatctattt aaagagaatg caatatatga
      181 tgattcactt tgcatattag gattgataaa aaataataga ttttatagat acataccaag
      241 taagtacata gaacaaaatg agaatttaaa tatgtacaaa gtaataatac caaagagtaa
      301 tggcacaggt gctttaggtg aaaccttgtc atctccttta ataggaaaac ctttaatcgg
      361 atatacacag tcttttattg gcataggagc ttttgacaat tatgatgaag caaaagcagc
      421 aaaaaagtac atagaaacta aatttgcaag agcaatgcta ggaattttaa aagtaactca
      481 agataataat agagatgttt gggaatatgt tcctttgcag gactttacaa aaaaatcaga
      541 tattgattgg aaccaaacta taaatgaaat agataggcaa ctctacgcta aatataatct
      601 cacaaatgat gaaattgcat ttattgaatc tatgataaaa ccaatggaat aaaagtgctt
      661 aattaaatta tcaaatatgg atgattacaa gtataggttc atagaaatag tcaaacgtgt
      721 tgaagattca aattgcgttt tttacaagaa ttgaagttgt tagaaaaaga attcagaagc
      781 ccaataattg aagcatttnn nnnnnnnnca ggttcttact tgaatgaaaa aatcagtagt
      841 gaaaaagagt tgtatagata tcttggaaat agatgtggaa aatacaaatc ttaaaaattg
      901 aatttggaat tttccgtaaa actttatgtg gtttatggta gaaataaaag tattatgaca
      961 aggttagaaa aattatatag cataatcgaa aattcgaaag agttaggttt gcaattaggc
     1021 gacgatgtgc ttcgccagac aagtgaactg gaagagaata taatcaagaa agaaattcta
     1081 ccagtattga cagaaacaat tgaaccagct cttagccaag tacgacgtga actggtgctt
     1141 gttgttgatt atgtgcctga tgaacctatt agcgtgagaa tttcgcgtaa gcgtaatttc
     1201 agtgcagaaa tggaagactc taaagagata ttacccgatc cacaagtgga acataatata
     1261 ggaataatca aacgtagagg tgacaaaaaa gctccacgca caaacctaag agtaaaaatg
     1321 gatgatggaa gaataataga acatcgcttt gctcttgata cattaaaaga agttgttcaa
     1381 atagtcggta tagataaagt cagagcactg aatataatac aatgtggtgt tccattgatt
     1441 tcaaacactc ttgataatgt ttattcttct tcacaaaagg atttacagaa cggttggtat
     1501 ctgatgacac tcagttctac aatcacaaag aaacagcaac tggataagat ctctgatgct
     1561 tataatttgg ggatggtagt ggaaatcgta taatgttttc tttcaagata atgagatcaa
     1621 aagtggaaca agccatattt ataaaaataa tttctttgaa aaatcttaat ggctcacctt
     1681 acaccagtat ttcagatttg gtattaacta tcatctcaca acttcccctc ccattccaaa
     1741 caaaattcat caataaggta aatgtcacta cgacattgta agtctgctat tccttcttgt
     1801 atcatctgga atgtagtgga atggaaaaac ctatccatcg cttcatca
//
LOCUS       DAOMTA010000678         1846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3194_length_1846_cov_2.512004, whole genome shotgun sequence.
ACCESSION   DAOMTA010000678 DAOMTA010000000
VERSION     DAOMTA010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_1846_cov_2.512004"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1846)
                     /locus_tag="PLK68_14415"
     CDS             complement(<1..>1846)
                     /locus_tag="PLK68_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91642.1"
                     /translation="IHSFFYRLPFTNIRPYRISPNTSFNSDELSTALINRDNVKNILD
                     LMNIALENTADIDNSYQQMVEHRELVLQYYTGVYTLFNEKKEIFDDDYEPILNAEESI
                     STRLYQFMKEHDPYDYMDQSSGNEEEDLNEVKAMLHDTNALTDTIKYLNLIIDENEDV
                     EEKGTAIMLREELSMIFALQRNHEYADKNEISYSFMGSISKEPRFNTGDFVFYSTYSR
                     DYYGTIEFINDDIVTIETWNGNETIEPIQIEIERNDFERVISQDFRNAYLFDESRPLY
                     RDEPSDIQDNDIPIYHTISKEIYKQVHNIAPLLLDGHSDYMAFKHIDESKILEFFIEN
                     DQLIIEEHDNDDTLLSSYILAIDPRDKLLNYRESTIDDEHYQLELYQDEPNDYEEELQ
                     ANERVQNYIEKTIHADAYRFYESRIDDNGTQISFSFDEHGELIQFNGSDIQFESFKQQ
                     FLITPIQSISCLLPVGILDIQEIKKPDNRSIAEKNFDLFNQIAPMVIDGSADYMYFVG
                     NSHDLPLTIETFEDRVMVSHDYEENDTVISDPLMEFVFDMDQQMMNARSIDQTKFNRY
                     LDVHVENDIVIDTKLEKELNQYANQWFHNLIEKDYHLKKMKVYINNYPT"
BASE COUNT          598 a          313 c          264 g          671 t
ORIGIN      
        1 ccgttggata gttgttgata tagactttca tcttttttaa atgatagtct ttttctataa
       61 ggttatgaaa ccactgattt gcgtattgat ttaattcttt ctctagtttt gtatcaatga
      121 caatatcatt ttctacatgc acatcaagat aacgattaaa ttttgtctga tctatacttc
      181 ttgcattcat catttgctga tccatatcaa atacaaattc catcaaagga tcagaaataa
      241 cagtatcgtt ttcttcatag tcatgactaa ccatgacacg atcttcaaag gtttcaatag
      301 ttaagggcaa atcatggcta ttgccaacaa agtacatata atcggctgaa ccatctatga
      361 ccatcggagc aatttgatta aataagtcaa agtttttttc tgcaatactg cgattatccg
      421 gtttcttgat ttcttgtata tctagtattc caacaggtaa aaggcatgaa atagattgga
      481 taggagttat caaaaactgc tgtttaaaag attcaaactg aatatcactc ccattaaatt
      541 gaatcagttc gccatgttca tcaaaagaaa aagagatttg tgtaccatta tcatcaattc
      601 gactttcata gaaacgataa gcatcagcat gaattgtttt ttcaatatag ttctgaactc
      661 tttcattcgc ttgtagttct tcttcataat catttggctc gtcctgatat agttctagtt
      721 gataatgttc atcatcaatt gttgattcac gataatttaa taatttatct cttggatcaa
      781 tcgcaagaat ataactggat aataaagtat catcattatc atgttcttca ataataagct
      841 gatcattttc aatgaaaaac tctaatatct ttgattcatc aatgtgctta aatgccatat
      901 aatcactatg tccatcaagc aataatggag ctatattgtg gacttgctta tagatttctt
      961 ttgagatggt atgatagatt ggtatatcat tgtcctgtat gtcacttggt tcatcacgat
     1021 acaatggtcg tgattcatca aatagataag catttctaaa gtcttgacta atcacacgtt
     1081 caaaatcatt acgctctatt tcaatctgaa ttggttcgat tgtttcattt ccattccaag
     1141 tttctatcgt aacaatatcg tcattaataa attcaatcgt accgtaataa tccctagaat
     1201 aggtagagta gaaaacaaaa tctccggtat taaatcttgg ttctttagaa attgaaccca
     1261 tgaaagaata actgatttca ttcttatctg catattcatg attacgctgt aacgcaaaaa
     1321 tcatggataa ttcttcacgc agcatgatag cagtaccttt ttcttcgaca tcttcatttt
     1381 catcaataat aagattcaaa tacttaatgg tatctgttaa agcatttgta tcatgcaaca
     1441 tggctttcac ttcatttaaa tcttcttcct cattgccgct gctttgatcc atataatcat
     1501 agggatcatg ttctttcata aactgatata agcgtgtcga aatactttct tctgcattta
     1561 aaataggctc ataatcatca tcaaaaattt ctttcttttc attaaataag gtatatacac
     1621 ctgtataata ctgtaaaaca agttcacgat gttctaccat ttgctgataa ctgttatcaa
     1681 tgtctgctgt attctctaaa gcaatattca ttaaatctaa gatattttta acgttatccc
     1741 gatttattaa tgcggtagaa agctcatcag aattaaatga tgtgtttggt gaaatacgat
     1801 aaggtcttat attggtaaaa ggaagtctgt agaaaaatga atgaat
//
LOCUS       DAOMTA010000679         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3200_length_1843_cov_2.040828, whole genome shotgun sequence.
ACCESSION   DAOMTA010000679 DAOMTA010000000
VERSION     DAOMTA010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3200_length_1843_cov_2.040828"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLK68_14420"
     CDS             complement(<1..581)
                     /locus_tag="PLK68_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRM91643.1"
                     /translation="MIRIAMAIKNQDIKNILKIELLKLCDDLQIMDYQEAVNYDVYMM
                     EIKNIAEIENLKRIRLEDYQQIIVLIGLEDLELIKKGYELEAFNYIRTNKFIEDIDNL
                     MSQLGDVMVKRFKTYQIQNSGTISKVRISSINYVESFRHYIHIHANSGEYIERKNISE
                     FVCQMKNENFIQIHKSYAVNLHAVIKIAANCVEL"
     gene            complement(574..1182)
                     /locus_tag="PLK68_14425"
     CDS             complement(574..1182)
                     /locus_tag="PLK68_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91644.1"
                     /translation="MDVKKYVIGKPRRILAIVNMLIWVPFATMLIVFEVQYHASIVNG
                     IVLGLVILSINFFIAGPIIYSSGMWWSIDEEVFKYSSFRKFTARIKAFYLPKQQNSYF
                     LALKVQQMDRIDLGWHDVPIFPFGLIGHPITFTITMKDGTTVELEALYTRESQKFVMA
                     CKHIESLGITLNDPYNLLEVIANPKRMVSQYIDEIESGVHHD"
     gene            complement(1281..>1843)
                     /locus_tag="PLK68_14430"
     CDS             complement(1281..>1843)
                     /locus_tag="PLK68_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM91645.1"
                     /translation="PYILIEAEKLARIFDRFMLCFISQENLNLIHGYAIKSHRIFGNF
                     IYGKFIDSVIIGLIALVGFGLMGLPFFPLLAFVVFITNMIPYFGPFIGGVPVVFIVLL
                     TNGIMPAIWTALFIFALQQFDGLILGPRILGDSVGISPFWIICSITIFGSLFGFLGMF
                     LGVPLICVIRMFFNDFLEYRKNRIKD"
BASE COUNT          673 a          331 c          227 g          612 t
ORIGIN      
        1 agttctacgc aatttgcagc gattttaata acagcatgaa gattaacagc ataagattta
       61 tggatttgaa taaaattttc atttttcatt tggcagacaa actcgcttat attttttctt
      121 tcaatatatt caccactatt agcatgaata tgaatgtaat gtctaaaaga ttcaacataa
      181 ttaattgaac ttattcttac tttagaaata gttccgctat tttgaatttg ataagtttta
      241 aatcttttta ccatgacatc accaagctga ctcatgagat tatcaatatc ttctataaat
      301 ttattggttc gaatataatt aaaggcttct aactcataac ctttttttat tagctctaaa
      361 tcttctaaac caatcaatac gatgatttgc tgataatcct ctaaacgaat cctctttaaa
      421 ttttcaattt cagcgatatt tttaatttcc atcatataaa catcataatt aactgcttct
      481 tggtaatcca tgatttgtag atcatcacat aacttaagca gttctatttt taagatattt
      541 ttgatatctt gattttttat tgccattgcg atcctaatca tgatgaaccc cgctttcaat
      601 ttcatcgata tattgagata ccatacgttt aggattagca ataacttcta ataaattata
      661 tggatcatta agcgttattc caagagactc aatatgttta caagccatta caaacttttg
      721 tgattctctt gtataaagtg cttctagttc tacagtagta ccatctttca ttgtaattgt
      781 aaaagttata ggatgcccaa ttaaaccaaa aggaaatatt ggaacatcat gccaacctaa
      841 atcaatccta tccatctgtt gaacttttaa agctaggaaa tagctatttt gctgtttagg
      901 aagataaaaa gctttgattc tagctgtaaa ttttctaaac gaactatatt taaatacctc
      961 ttcatcgata ctccaccaca ttccagagct ataaattatt ggaccagcaa taaaaaaatt
     1021 aatacttaaa ataacaaggc ctaaaacaat tccattgacg attgaagcat ggtattgaac
     1081 ttcaaataca atcaacattg tcgcaaatgg tacccatatc aacatattga caatagctaa
     1141 aatccttcgc ggttttccga ttacatattt tttcacgtcc atctcctcct aattttattt
     1201 tatctaatta ttattaaaat gatatactta tgcacgtatt gagatttact ggcacaaaaa
     1261 aagattaatg attctgctca ttaatcttta atacgatttt ttcgatattc taggaaatca
     1321 ttaaagaaca tccgaattac acaaattaac ggaactccta aaaacattcc caagaatcca
     1381 aagagactcc caaatatggt aattgagcaa ataatccaaa atggtgatat tcctacacta
     1441 tcgcctaaaa tgcgcggtcc taaaattaag ccatcaaact gctgtaacgc aaaaatgaaa
     1501 agagcagtcc agattgctgg cattatccca tttgttaata atacgataaa aactacgggc
     1561 accccaccta tgaatggtcc aaaataagga atcatgttgg taataaaaac aacaaaagct
     1621 aataatggga aaaacggtaa gcccatcagt ccaaatccga ctagtgcaat caatccaata
     1681 atgactgaat caatgaattt cccataaata aaattaccaa aaatacgatg tgatttaatc
     1741 gcataaccat gaattaaatt caaattttct tgactaataa aacaaagcat aaaacgatca
     1801 aaaatacgtg caagtttttc tgcttcaatc aaaatatatg gac
//
LOCUS       DAOMTA010000680         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3220_length_1838_cov_1.744251, whole genome shotgun sequence.
ACCESSION   DAOMTA010000680 DAOMTA010000000
VERSION     DAOMTA010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3220_length_1838_cov_1.744251"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>265
                     /locus_tag="PLK68_14435"
     CDS             <1..>265
                     /locus_tag="PLK68_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRM91646.1"
                     /translation="GERAEVVGNFFSQFNDIMMEMTMMIMAIAPLGVFCLIARTFAGI
                     GFGAFLPLGKYMIAVLIALAVQCLFVYLGLLKLFTGLNPIKFIK"
     assembly_gap    266..302
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <303..786
                     /locus_tag="PLK68_14440"
     CDS             <303..786
                     /locus_tag="PLK68_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRM91647.1"
                     /translation="AFAFSTATSNATIPLSIDTLAKKMGVSKRISSFTIPLGATINMD
                     GTAIMQGVAVVFAAQAFGINLDLTDYITVIGTATLASIGTAGIPSVGLVTLTMVFNSV
                     GLPVEAIGMIMGIDRILDMTRTAVNITGDAVCTTIVAHQNKAINRNIFDNSDIKRVSE
                     "
     gene            852..1628
                     /locus_tag="PLK68_14445"
     CDS             852..1628
                     /locus_tag="PLK68_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRM91648.1"
                     /translation="MNKLSGLKTDCCGCRGFCCRALFFFKSDGFPYDKPAKRNCKNLL
                     ADYRCKIHDQLEIVNYYGCINYDCLGAGQKTALFKEDDQLLFEVFEVMVQLHEILWYL
                     YVGKNFVQSQRLNEQLETYYQMIESLTYLDAEKILALDLNKYRQLVNPLLQEVYCQMN
                     KDIQIKPLQARKTVLNRADFMGCDLHEKDLRGQDFKGAFLIGANLSNCDLCGVNFLGS
                     DVRKMDIRNSDLSKSLFLTPAQISACVINRYTKLPEYIGY"
     gene            1656..>1838
                     /locus_tag="PLK68_14450"
     CDS             1656..>1838
                     /locus_tag="PLK68_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91649.1"
                     /translation="MDRVGIKLWAKEKTRSNKWNIWKGFLAIFAASLGLSFIALLLFS
                     VMIDIGGSSYYDTFTFM"
BASE COUNT          591 a          222 c          375 g          613 t
ORIGIN      
        1 ggtgaacgtg ctgaagtggt tggtaacttt ttcagtcagt ttaatgatat tatgatggag
       61 atgactatga tgattatggc aattgcacca ttaggagtat tttgtttaat tgcaaggact
      121 tttgcgggaa taggatttgg tgctttttta ccattaggta agtatatgat tgctgtcttg
      181 atagcattgg cggttcagtg tttatttgta tatttaggct tactcaaatt atttacggga
      241 ttaaacccga ttaaatttat taaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnggcttttg ctttttcaac tgccacttca aatgcgacca tccctttatc aatagatacg
      361 ttagctaaaa aaatgggagt ttcaaagaga atttcctcat ttacaattcc tttaggggct
      421 acgatcaata tggatggtac ggcaatcatg cagggagtgg cagtcgtctt tgcggcacaa
      481 gcctttggaa tcaatctaga tctaacagat tacataaccg taattggaac agctacatta
      541 gcttcaattg ggacagcggg aattccaagt gttggattag ttactttaac gatggtattt
      601 aattcagttg ggttgccagt ggaagcgatt gggatgatca tgggaattga ccgtatttta
      661 gatatgacac gaacagcagt taacattaca ggtgatgctg tttgtacaac aattgtggca
      721 catcaaaata aagcaatcaa tcgtaatatt tttgataata gtgatataaa aagagttagt
      781 gaatagtttg taataatcat gtataataaa aggtgaagat aggatcttca tcttttattt
      841 tggaggatat tatgaataag ttatctggtt tgaaaacaga ctgttgtggt tgtagaggat
      901 tttgttgtcg agcactattc ttttttaaaa gtgatggttt tccttatgat aaaccagcaa
      961 aaagaaattg taaaaattta ttagcagatt atcgttgcaa aatacatgac cagctagaaa
     1021 ttgtaaatta ttatggttgt ataaactacg attgtcttgg agcgggacaa aagacagccc
     1081 tgttcaaaga agatgaccag cttctatttg aagtatttga agttatggta cagctgcatg
     1141 aaattttatg gtatttatat gttggtaaaa attttgtaca aagtcaacga ttaaatgaac
     1201 agctcgaaac atattatcag atgattgaat cattaactta tttagatgcc gaaaaaatcc
     1261 ttgcacttga cttaaataag tatcggcaat tagtaaatcc tttattacag gaagtctatt
     1321 gtcaaatgaa taaagatatt caaattaaac cattgcaagc tagaaaaaca gtactgaatc
     1381 gtgctgattt tatgggttgt gatttacatg aaaaagattt acgtggacaa gattttaagg
     1441 gggccttttt aattggggca aatttgtcta attgtgattt atgtggtgtt aattttttag
     1501 gaagtgatgt aagaaagatg gatattagaa atagtgattt atcaaaaagt ctatttctaa
     1561 caccagctca aataagtgca tgtgttatta atcggtacac aaaattgccg gaatatatag
     1621 gctattagta aatttgtaag agggagaaga taaagatgga cagagtagga attaaattat
     1681 gggctaaaga aaaaacacga tcgaataaat ggaatatttg gaaaggtttt ttagctatat
     1741 ttgcggcttc actaggactt tcttttattg ctttattact attttcagta atgatcgaca
     1801 ttgggggaag cagttattat gatactttta cttttatg
//
LOCUS       DAOMTA010000681         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3225_length_1837_cov_2.401235, whole genome shotgun sequence.
ACCESSION   DAOMTA010000681 DAOMTA010000000
VERSION     DAOMTA010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_1837_cov_2.401235"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /gene="galE"
                     /locus_tag="PLK68_14455"
     CDS             <1..706
                     /gene="galE"
                     /locus_tag="PLK68_14455"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRM91650.1"
                     /translation="NNLTTTLIVSKMMKKYHVNQIVFSSSATVYGDPETVPITEDCKL
                     GETTNPYGTSKAMMERILTDVQHACPEMSVTLLRYFNPIGAHESGLIGEDPKGIPNNL
                     MPYIMKVATGELECLGVFGDDYDTHDGTGVRDYIHVVDLAKGHVKAIEHYANPGVHIC
                     NLGTGTGYSVLDLVKAFERVNNVKVKYVIKDRRPGDIATCYANPARAKEELDWVATKG
                     IDEMCRDTWNYALKHK"
     gene            805..1224
                     /locus_tag="PLK68_14460"
     CDS             805..1224
                     /locus_tag="PLK68_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91651.1"
                     /translation="MEKVKKLWKEISIFGIVLVVFIGLFVYRKITHVEYTTITQSALV
                     EKVKDKDSFVVVIGNNSDNTTLSYQQTMTTFVEKNRSKSLYYVDVSGDSDYTTWLEEK
                     LDITDGTVPQTLVYEKGKVKTAKTGALSYYRLSQLYK"
     gene            complement(1300..>1837)
                     /gene="alr"
                     /locus_tag="PLK68_14465"
     CDS             complement(1300..>1837)
                     /gene="alr"
                     /locus_tag="PLK68_14465"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM91652.1"
                     /translation="ELFYDLVADHKFKYIHCCNSAAMAYHHDDRSNLGRIGIIMYGID
                     PAGNETKEFKQVMSLYSKVALIKKIKAGDRVGYGLTYTADEDEYLATIPIGYADGLIR
                     KNQGRNVYINGKYFEIVGRVCMDQIMVRVDETIKEGDKVEIFGAHISLASMARELETI
                     PYEIICLITKRVDRLYIK"
BASE COUNT          634 a          307 c          302 g          594 t
ORIGIN      
        1 taataactta acaacaactt tgatcgttag taaaatgatg aaaaaatatc atgttaatca
       61 aattgttttt tcatctagtg ctacagttta tggtgaccca gaaaccgttc caattactga
      121 agattgtaag ttaggtgaaa caactaaccc atacggtact agcaaagcaa tgatggaaag
      181 aattttaact gacgttcaac atgcatgccc agaaatgtca gtaacattgt tacgttactt
      241 taatccaatc ggagcccatg aatctggatt gattggtgaa gatcctaaag ggattcctaa
      301 caatttaatg ccatatatca tgaaagttgc aactggtgaa ttagaatgct taggtgtatt
      361 tggtgatgat tatgatacac atgacggaac tggcgttcgt gattatattc atgttgttga
      421 cttagctaaa gggcatgtaa aagcaattga acattatgct aatcctggag tccatatttg
      481 taatcttggt acaggtacag gatatagtgt tcttgattta gttaaagctt tcgaaagagt
      541 caataatgta aaagttaaat acgttattaa agatcgtcgt cctggggata tcgctacttg
      601 ttatgctaat cctgctcgcg ccaaggaaga gcttgattgg gtagctacca aaggaattga
      661 cgagatgtgt cgcgacactt ggaattatgc tttaaaacat aaataagttt aactatggga
      721 tatgtcacat catatccctt tattatatat tgtttttagc tgaaatattt gttattatat
      781 aaaggtatat tatggaggct aatcatggaa aaagtaaaga aattgtggaa agaaattagc
      841 atctttggaa tcgtattagt agtgttcatt ggattatttg tatatcggaa aataacccat
      901 gttgaataca caacgattac tcaaagtgca ttagtagaaa aagttaaaga taaggatagt
      961 tttgtcgtag ttatcggaaa taatagtgat aatacaacat tatcatatca acaaaccatg
     1021 accacatttg tagaaaaaaa tcgtagtaaa tctctatact atgtagacgt ttctggtgac
     1081 agtgattata ctacttggct agaagaaaaa ctcgatatca ctgatggtac ggtaccacaa
     1141 acattagtat atgaaaaagg aaaagtcaaa acagctaaga ctggtgcttt atcatattac
     1201 cgattaagtc aattgtacaa ataaaaaaca gcataatgat gtatctatta tagtcagtct
     1261 ctcgctaaca tttagatttt acatcattgt actgtttttt tattttatat ataaacggtc
     1321 aactcgttta gtaatcaaac aaattatttc ataaggaatc gtttcaagtt ctcttgccat
     1381 gctcgctaaa cttatatgcg ctccaaaaat ctctacctta tccccttctt taatcgtttc
     1441 atctacccga accattattt gatccatgca aacacgacca acaatttcaa aatatttacc
     1501 attaatataa acgttccgcc cctgattttt acgaatcaat ccatcagcat agccaattgg
     1561 gatcgttgct aaatattcat cttcatcagc agtgtaagtc aagccgtaac caactcggtc
     1621 accagcttta atttttttaa tcagagcaac tttagaatat aaagacatta cctgtttaaa
     1681 ttcctttgtt tcattaccag ctggatcgat cccatacata ataattccaa ttctccctaa
     1741 attagaacga tcatcatgat gataagccat tgccgcacta ttacaacaat gaatatattt
     1801 aaatttatga tcagctacta aatcataaaa aagttct
//
LOCUS       DAOMTA010000682         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3242_length_1830_cov_2.589296, whole genome shotgun sequence.
ACCESSION   DAOMTA010000682 DAOMTA010000000
VERSION     DAOMTA010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_1830_cov_2.589296"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1830
                     /locus_tag="PLK68_14470"
     CDS             15..>1830
                     /locus_tag="PLK68_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM91653.1"
                     /translation="MGGFASYRVNKKFEQIKPKNGEHHYTNEHILICLSPAPSNQKVI
                     RTAARMANAFFGEFTAVFVETPNFEKMPTKVKQALRANTKLASQLGANIVNLYGDNVP
                     EQISEYARFAGVSKIVLGRTNTKRRFSHNSFADQLIALTPTIDIYIIPDKIRNNYKHT
                     SLKPRQFFSLSIKDTILSIVIILIVTLLGIWFRDLGFGEANIIMVHILGVLATSLLTE
                     NLIYTLLSSLVSVLSFNFFFTIPMHSFTAYDKGYPITFVIMFLTAFITGTLTKRVKEQ
                     ARLSAIKAFRTEVLLKSSQKLQRAKTKKEIIDEISKQLFKLLDKTIIFYPVENHELSE
                     PLVFKSNEQEDEKIYLNKDEEAVASWVFNNNKHAGASTTTLPGAKCLYLAVRCEDSVL
                     GVIGIYLDKTAIDDFENNLLMAILNEGAMALEKEASNSKKREVEIKANQEELRANLLR
                     SISHDLRTPLTSISGNAGVLLDSADKLSNERKIEIYSDIYEDSMWLIDLVENLLSITR
                     IENGNIQINKEAQLINEIVLEAMHHISKDSADHIIQLDLSDEFIFVKIDARLIMQVII
                     NIVNNAIKYTPLGSTIKITTKKLNQVLSLEISDDGEGVP"
BASE COUNT          666 a          252 c          311 g          601 t
ORIGIN      
        1 acgagactta tagattgggt ggttttgcat catatcgtgt taataaaaaa tttgagcaaa
       61 ttaaacctaa aaacggcgaa catcattata ccaatgaaca tattctaatt tgtttatcac
      121 cagctccttc aaatcaaaaa gttattagaa cagccgctcg aatggctaat gctttctttg
      181 gtgaatttac agcagttttt gtagagacgc ctaattttga aaaaatgccg actaaagtta
      241 aacaggcatt aagagccaat accaaattgg cttctcaatt aggggctaat atagtaaatt
      301 tgtatggtga taatgttcct gaacaaatta gtgaatatgc aaggtttgct ggagtatcaa
      361 aaatcgtatt aggaagaact aatactaaac gacgtttttc gcacaatagt tttgcagatc
      421 aattaatcgc tttaacacca acgatcgata tttatatcat cccagataaa attagaaaca
      481 attataagca tacgtcttta aaaccgcgac aatttttttc tttatcaatt aaagatacca
      541 tcctttcaat tgtaattatt cttattgtta cattattagg aatttggttt agagatttag
      601 gatttggtga agctaatatt attatggtgc atattctagg tgttttagca acttcattat
      661 taacggaaaa tctaatttat actcttttgt cgtctttagt aagtgtgcta tcttttaatt
      721 tcttttttac tattccgatg cactcattta cagcatatga taaaggttat ccaataactt
      781 ttgtaatcat gtttttaaca gcttttatca caggtactct aactaaacga gtaaaagaac
      841 aagctcgtct atcagcaata aaagcttttc gaacagaagt tctattaaaa tcgagtcaaa
      901 aattgcaacg ggctaaaaca aaaaaagaaa taattgatga gatttctaaa caattattta
      961 aacttctaga taagacaatt attttttatc ctgttgaaaa tcatgaacta agtgaaccac
     1021 tcgtatttaa aagtaatgaa caggaagatg aaaaaattta tttgaataaa gatgaggaag
     1081 cggtagcttc ttgggttttt aataataata aacatgctgg tgcttcaaca acgacgttac
     1141 ccggagcaaa atgtctatac ttagctgttc gttgtgaaga tagtgttttg ggagtaattg
     1201 gaatctattt agataagaca gcgatcgatg attttgaaaa caatttatta atggctattt
     1261 taaatgaagg agcgatggct ttagaaaaag aagcttcaaa tagtaaaaaa cgtgaagttg
     1321 aaattaaagc gaatcaagaa gaactacgtg caaatttact acgctctatt tctcatgatt
     1381 tgcgtacacc attgactagc atttctggca atgcgggagt tttattagat agtgcagata
     1441 aactatctaa tgaacgtaaa atagaaatat atagtgatat ttacgaagac tcaatgtggt
     1501 tgattgattt agtagagaat ttactttcaa taacaaggat tgaaaatggg aatattcaaa
     1561 taaataagga agcacaatta attaatgaaa ttgttttaga agcgatgcat catattagta
     1621 aagatagtgc agatcacatt attcaattgg acttatcaga tgagttcatt tttgtaaaaa
     1681 tagatgcccg actgattatg caggttatta ttaatattgt aaataatgca ataaaatata
     1741 caccgttagg ttcaactata aaaattacaa ctaagaagct taatcaggta ttatcactag
     1801 aaatcagtga tgatggagaa ggcgttcctg
//
LOCUS       DAOMTA010000683         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3248_length_1828_cov_2.245911, whole genome shotgun sequence.
ACCESSION   DAOMTA010000683 DAOMTA010000000
VERSION     DAOMTA010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_1828_cov_2.245911"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /gene="cysK"
                     /locus_tag="PLK68_14475"
     CDS             <1..744
                     /gene="cysK"
                     /locus_tag="PLK68_14475"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRM91654.1"
                     /translation="KPDSVIIEPTSGNTGIGLASLGTSLGHQVILTMPETMSIERRNL
                     LKAYGAKVVLTPGGLGMKGAIAKAEELAKEYKNAFIPSQFENQANPNAHYLTTGPEIY
                     QQLEGKIDIFVAGVGTGGTISGIGKYLKEKNPNIKVVAIEPAASPVLSKGTPGPHAIQ
                     GIGAGFVPNTLNTDIYDEIITIENEAAFATSRAIAREEGVLVGISSGAALYGATVLAK
                     RIENAGKNIVVLLPDTGERYLSTALVEQD"
     gene            complement(1358..>1828)
                     /locus_tag="PLK68_14480"
     CDS             complement(1358..>1828)
                     /locus_tag="PLK68_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM91655.1"
                     /translation="NPSNPLAHEETTALEIIDDFDDNIDYFVAGIGTGGTITGIARIL
                     KKKYPDIKIIGIEPKDSPLITKGKAGSHDLQGIGANFIPKILDLDLVDEVITVSTDDA
                     YEAARILAKKEGLLVGITAGAALHGATKITDKNKNIVVLLPDTGERYLSTTLFE"
BASE COUNT          643 a          266 c          324 g          595 t
ORIGIN      
        1 aaaccggatt ctgtaatcat tgaaccaacg agcggtaata ctggaattgg attagcttct
       61 ttaggaacat ccttaggaca ccaagtaatt ttaactatgc cagagacaat gtcgattgaa
      121 cggcgaaatt tattaaaagc ctatggagct aaagtagttt taactccagg aggactaggc
      181 atgaagggag caattgccaa agcagaagaa ttggccaaag aatacaaaaa tgcgtttatt
      241 cctagtcaat ttgaaaatca agcaaatcca aatgcacatt atctaacaac aggtcctgaa
      301 atctaccagc agctagaagg taagatagat atctttgtcg ctggtgttgg aactggagga
      361 acaattagtg gaattggaaa atatttaaaa gaaaaaaatc ctaatattaa ggttgtagca
      421 attgaacctg ctgcctcacc agttttatct aaaggaactc caggaccaca tgcaattcaa
      481 gggattggtg caggttttgt tcctaatacc ttaaatactg atatttatga tgaaattatt
      541 acaattgaaa atgaagcagc ctttgctact agccgtgcaa ttgctagaga agaaggagtg
      601 ttagtaggta tttcttctgg tgcagcactt tatggagcaa cagtattagc gaaaagaatt
      661 gaaaatgctg gcaaaaacat tgttgtgttg ttaccagata ctggtgaacg ctatttatca
      721 actgcattag ttgaacaaga ttaataatat ggatactgtc tttagggaca gtatcttttt
      781 ctaactacaa aaataatttt taattataat tatattttaa aagtctatca taaaaatgtg
      841 agtgtttgtt atgcaactag gataaacaac taataattta aagtttaata atgagattca
      901 ctatttaaaa atataaatag acgggaagaa gagcagagaa agatgattag ttgtaatgag
      961 gtgattgtag tcaatatcaa tattagccaa aaagattgtc caaaatatct ttaaattatg
     1021 gacaatgttt tttttgttat agtatacaaa aaagtacttg gaatatttgt aattgatatc
     1081 ttaataaaca gaaattatat cattgaggtg atatgataag ttgtcttgta tggacgagct
     1141 taaacaaaaa agttcagtta aaatcctgtt gatttgaaaa agtaattagt acagttaaaa
     1201 agaataaatc aaaattaaag ttatagagga taatgtattg gttattataa taataagata
     1261 tcattctaca ttttcactgg acatttagga aaataaaaaa catagtctga agagcattgt
     1321 gtaaaatact tttaatgcta ctcaattgct atgcctttta ttcaaataat gttgttgata
     1381 agtaacgttc tccagtatct ggcagtagaa caacgatgtt tttattttta tctgttattt
     1441 tagttgctcc atgcagggca gctccagcag ttattccaac tagtaggcct tctttttttg
     1501 ctaatattcg tgcagcctca taagcatcat ccgtagaaac tgttatcact tcgtcgacta
     1561 gatccaaatc gagaatttta ggaataaaat ttgctccaat tccttgcaaa tcatgcgatc
     1621 cagcttttcc tttagtgatt aatggagaat cttttggttc gattccaata attttaatat
     1681 caggatattt cttttttaat atccgagcaa ttcctgtaat tgtccctcca gtaccaattc
     1741 cggcaacaaa atagtcaata ttatcatcaa aatcatcaat aatttctaat gccgttgttt
     1801 cttcatgggc taagggatta cttggatt
//
LOCUS       DAOMTA010000684         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3254_length_1826_cov_2.617165, whole genome shotgun sequence.
ACCESSION   DAOMTA010000684 DAOMTA010000000
VERSION     DAOMTA010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_1826_cov_2.617165"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLK68_14485"
     CDS             complement(<1..358)
                     /locus_tag="PLK68_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRM91656.1"
                     /translation="MSKKLVAYFSASGVTKKVALRLAQEELAELFEICPKIEYTSADL
                     NWMDDNSRSSLEMKAQSCRPEILNKLDNLDQYEIIYLGFPIWWYVEPRIIDTFLESYN
                     FSKKQIVPFATSGGSGI"
     gene            complement(469..1773)
                     /locus_tag="PLK68_14490"
     CDS             complement(469..1773)
                     /locus_tag="PLK68_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM91657.1"
                     /translation="MDKFQDILMKVGVFAAENRYLSSIKNAFQTFVPFTIIGAIGVLW
                     SNVICNDTTGLGALVPAVMNLSFLNPAFNALNFATIGCISVAITFLVGGEIGTSRKSS
                     PMFCGLLAVVSLLTVTQTSLDIKAGGELIQTVSGIFTSSLGSQGLFTGMIVAIVAVEL
                     FCGLFKLDKLKIKLPDQVPPQIAKSFEYLVPAFIEILIISLVGLGVNAVSGVYINDVI
                     FNVIQKPLLYIGGSLPGVLTFMFISLVFWSIGLHGDNMIGGVFNPILTTLAVENLDAI
                     KAGLEPSNIVNNTFHRAFFATGGTGCMLGLTIAMLIVCKRPENKSIARIALVPELFNI
                     GEVSMFGVPIVMNPTLIIPFILAPLVTVIFGYVLTMLHICPIMYVDLPWTMPPLLIAF
                     LGSGGNFMAPICQLAGIILSALIYLPFVKLYEKQQAQIEQTA"
BASE COUNT          664 a          355 c          234 g          573 t
ORIGIN      
        1 tgataccgct acctccagat gtagcgaaag gtacaatttg ttttttagaa aaattataac
       61 tttccaaaaa ggtatcaata attcgaggtt caacatacca ccagattgga aaacctagat
      121 agattatctc atattgatca agattatcca atttgtttaa aatctctggt ctacagcttt
      181 gcgccttcat ttctaaagaa ctgcgactat tatcatccat ccaatttaaa tcagcacttg
      241 tatactcgat ctttggacaa atttcaaata attccgctaa ttcttcttga gccagtctaa
      301 gtgcgacttt ttttgtaaca ccactagcac taaaatacgc aactaatttc ttactcatac
      361 atttcctcct aaacaataat aaaggtatat ctctatacct ttattataag atgactttaa
      421 ataaagtcta atacttagtt tttattagta gtaatgctta ttaagcatct atgctgtttg
      481 ttcaatttgt gcctgttgtt tttcatatag cttaacaaaa ggtaaataga tcaatgcaga
      541 taaaatgatt cctgctaatt gacaaattgg cgccataaaa ttacccccag atcctaaaaa
      601 tgcaattaac aacggtggca ttgtccacgg taaatcgaca tacataattg gacaaatatg
      661 aagcattgta agaacataac caaaaataac agtaactagc ggtgctaaaa taaatgggat
      721 gattaaagtt ggattcatta caataggaac cccaaacata ctaacttcac caatattaaa
      781 taattcaggt actaaagcaa ttcgtgcaat tgatttattt tctggacgtt tacaaactat
      841 taacatagct atcgtcaaac ctaacataca tcctgttcca ccagtagcga agaaagcccg
      901 atgaaatgta ttattgacaa tattacttgg ctccaaccct gctttaattg catcaagatt
      961 ttcaacagct aaagttgtta aaataggatt gaaaacacca ccaatcatgt tatcaccatg
     1021 taatccaatt gaccaaaata ctaaactaat aaacataaat gttagtacac ccggtaaaga
     1081 accacctata tatagtaatg gcttttgaat aacattaaaa ataacatcat taatgtagac
     1141 tcccgatacc gcatttaccc ccaaaccaac taaagaaata atcaaaattt caataaatgc
     1201 aggtactaaa tattcaaatg atttagcaat ttgtggtggt acttgatctg gtaatttgat
     1261 ttttaactta tcaagtttaa ataatccaca aaataattct actgccacta tcgctacgat
     1321 cattcctgta aatagtcctt gactccctaa tgaactagta aagattccac tcacagtttg
     1381 aattaattca cctcccgctt taatatctaa tgatgtttga gttacagtta ataatgaaac
     1441 gactgctaat aaaccacaaa acattggact gctttttcga cttgttccaa tttcaccacc
     1501 aactaaaaat gtgattgcaa cactgataca tccaattgtc gcaaaattta gtgcattaaa
     1561 agctggattt aaaaaactta aattcattac cgctggtact aaggccccta atcccgtggt
     1621 atcattacaa ataacatttg accataatac accaattgct ccaataatag taaatggaac
     1681 aaaagtctga aatgcattct tgattgatga aagataacga ttttcagcag caaaaacgcc
     1741 tactttcatc aatatatctt gaaatttatc cataaaattc ttctcctctt ttaaaaacat
     1801 tgtacttaat cttcgctagc agataa
//
LOCUS       DAOMTA010000685         1822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3266_length_1822_cov_2.357668, whole genome shotgun sequence.
ACCESSION   DAOMTA010000685 DAOMTA010000000
VERSION     DAOMTA010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3266_length_1822_cov_2.357668"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..687
                     /gene="tnpA"
                     /locus_tag="PLK68_14495"
     CDS             175..687
                     /gene="tnpA"
                     /locus_tag="PLK68_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRM91658.1"
                     /translation="MKDTKSLSHTSYRCKSHIVIVPKFRRMAIYNKLRHDIRLIIKTL
                     IERKPGVELIEGELCPDHIHLLLEIPPKYSVAEFMGYLKSKSTLMIFDRHASMKYKYG
                     NQKFWARGYFVDTVGKNEKTVREYIQNQLAEDKLSNQMSMEEFIDPFTGEPVEGDKKK
                     KKSKRPFEGQ"
     gene            complement(797..>1822)
                     /locus_tag="PLK68_14500"
     CDS             complement(797..>1822)
                     /locus_tag="PLK68_14500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM91659.1"
                     /translation="IVIVVSINVILNKKVLKGIHSVLNDLNRIKNGDLNTVVKADDNQ
                     ELIDLSSGINSMVNSVVNSADRISKIISIIDIPLAAFEYQNDTKQLFATARLKELLHL
                     SDEEANQMYLDPKQLLKKLQIIISNPIEGESDVYCLEKDTFIKIRLVKDENGFYGTVN
                     DVSSDILKKKQIQYEKDHDHLTGLLLYPSFKREVTLVINNNNYNHLYAAVMVDLDSFK
                     HINDTYGHDFGDHYLKRLTYALNKLSKSNCLIARRSGDEFCLFIYNYQDKNTIINQLN
                     KLWSYFKEELIELPDHSYQSIKVSGGFVCSSGPNNTIEELMKKSDKALYEAKNHYKGH
                     FIEYKGK"
BASE COUNT          635 a          266 c          304 g          617 t
ORIGIN      
        1 cctaataata tttgtttaac tttcaacaaa tattagtttt ttcttattgt gaaacaaaaa
       61 ccacgtgcta tgcgcgtggt aacaataaag ctttagcgtc tgggtaaaaa acctcctcgt
      121 gatataataa ttgtagcctg gcagctgcaa ataaaataac gaggaggtta gtctatgaaa
      181 gacacaaaaa gtttatcaca tacatcatat agatgtaaat ctcatattgt aatagtacca
      241 aaatttagaa ggatggcaat atacaataaa ttaagacatg atataagatt aattataaag
      301 accttaattg aaagaaaacc gggagtagag ctgatagaag gagagttatg tcctgatcat
      361 attcaccttc tattagaaat accaccaaag tattctgtgg cggagttcat gggatacctg
      421 aaaagcaagt caacgcttat gatatttgat cgtcatgcaa gtatgaaata taaatatgga
      481 aatcaaaaat tttgggcaag aggctatttt gtagatacag taggaaagaa cgagaagaca
      541 gtgagagaat atattcaaaa tcagttggcg gaagataagt tgagtaatca aatgagtatg
      601 gaagaattta ttgacccgtt cacaggagaa ccagtggaag gggacaagaa aaagaaaaaa
      661 agtaaaagac cctttgaggg tcagtgagca aatggcatta cggctgtcaa ggcgttcagt
      721 ggtcgaatga ctagcgcaac agcatgccct tacagggcga gtacgtcttt taggcgtggt
      781 tatgatttac tatcaactat tttcctttat attcaataaa atgaccttta tagtggtttt
      841 ttgcttcata cagagcttta tcagatttct tcattaattc ttcgatagta ttatttggac
      901 cactgctaca tacaaagcct ccagatactt ttattgattg atatgaatga tcaggaagtt
      961 caattaattc ttctttaaaa taagaccata atttatttaa ttgatttatt atagtatttt
     1021 tatcttgata attataaata aagagacaaa attcatcacc agatcgtcgt gcgattaaac
     1081 aatttgattt agaaagctta tttaatgcgt aggtcaaacg tttcaaataa tgatcaccaa
     1141 aatcatgacc ataagtatca tttatatgtt taaatgaatc taaatcaacc attacagctg
     1201 catataaatg attataatta ttgttattaa tgactaaagt tacttctctt ttgaatgatg
     1261 ggtatagtaa tagtccagtt aaatgatcat gatctttttc gtactgaatt tgttttttct
     1321 ttaaaatatc agaagaaaca tcattaacag ttccatagaa accattttca tctttcacaa
     1381 gtcggatttt tataaaagta tctttttcaa gacaataaac atcactctca ccttcaattg
     1441 gattgctaat aattatttgt agttttttta atagttgttt tggatcaaga tacatttgat
     1501 ttgcttcttc atcacttaaa tggagcagtt ctttcaatct tgcagtggca aaaagttgtt
     1561 tcgtatcatt ttgatattca aatgctgcta gtgggatatc gataatggag ataatttttg
     1621 atattcgatc agcggagttg acaacactat taaccatact gttgattcca gagcttaaat
     1681 ctatcaattc ttgattatca tcagctttta cgacagtatt taaatcgcca ttttttattc
     1741 tatttaaatc attgaggaca ctatgaattc cttttaatac ttttttgttt aatataacat
     1801 tgattgacac aacgataacg at
//
LOCUS       DAOMTA010000686         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3268_length_1821_cov_2.218573, whole genome shotgun sequence.
ACCESSION   DAOMTA010000686 DAOMTA010000000
VERSION     DAOMTA010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3268_length_1821_cov_2.218573"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..725
                     /locus_tag="PLK68_14505"
     CDS             33..725
                     /locus_tag="PLK68_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91660.1"
                     /translation="MIEVNGLTKIYDGFRAVDNVDLVAKGGKVTILLGPNGAGKSTTI
                     KSIANLLKFDGEIKICGYPNDSIEAKRCFGYVPETPILYDLLTIDEHIDFIGNAYRVD
                     NYHEIANKYLELFKLTGKRKSMAKELSKGMTQKLSMLLALLIQPQALLVDEPMVGLDP
                     ASIEDVLKIFTLLKEEGCAVFVSTHIIDIIKDIYDEAYIMNKGKIIKHVLRDELEDES
                     LKQYFFELTDGE"
     gene            727..>1821
                     /locus_tag="PLK68_14510"
     CDS             727..>1821
                     /locus_tag="PLK68_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HRM91661.1"
                     /translation="MKPLLKLWLLKIKGTIRNLFKRKASGVFVIIMILFYGAMIVSLF
                     NVDAGQIMAVNNIDLHMGILLLIGFQAIMLFATLMQSKKALFTGEDAFYLFTGPFTRR
                     QVMSYLTFQTIIQAFLLALISLVFLAAFSGGAGFNFIFIVLAYLASVITVLFFLLLTD
                     YLYVLSIGDKKYRKYSKIIPGIIIAFVVIIVLVLYLQTGNYHTLFMDFVQSNLFYVVP
                     IFGWMKLALIAYVEHNYLLVTLGYLLLCGAVILVYVLFIGYRGNFYEQALQDSLDLSK
                     RMKAAKAGDQEALRNKKVKLGIKGEFRQGAYAVMSKNILLMRKTNSFISVSDLISIGI
                     YIAVTIAVDVGFGMFIYMMVIWIFSSLQNSD"
BASE COUNT          575 a          195 c          364 g          687 t
ORIGIN      
        1 atagtgaata tatagactag ggggaatttg aaatgattga agtaaatggt ttaacaaaaa
       61 tttatgatgg ctttagagct gttgataatg ttgatttagt tgctaaaggg ggaaaagtca
      121 caatcttact tggaccaaat ggggctggta aatcaacaac gatcaaaagt attgctaatc
      181 ttttgaaatt tgatggagag attaaaattt gtggttatcc taatgatagt attgaggcta
      241 aacgttgttt tggatatgtt ccagaaacac cgattttgta tgatctgctt actattgatg
      301 agcatattga ttttattgga aatgcgtatc gagtagacaa ttatcacgag attgcgaata
      361 aatatcttga attatttaaa ttaacaggga aaaggaaaag catggctaaa gagttatcca
      421 aagggatgac acagaagctg agtatgttat tagcactttt aatccagcca caagctttat
      481 tagtcgatga accgatggtg ggattagatc cagctagtat tgaagatgtt ttaaaaattt
      541 ttacgttgct aaaggaagaa ggttgtgcag tttttgttag tacccatatc attgatatta
      601 ttaaggatat ttatgatgaa gcttatatta tgaataaagg taaaattatt aaacacgttt
      661 tacgtgatga attagaagat gaatcgttaa aacaatattt ctttgagtta acggatggtg
      721 agtaagatga aaccgttatt aaaactatgg ttattaaaaa tcaaagggac aatcagaaac
      781 ttatttaaac gtaaagcaag tggagtattt gttattatta tgattctatt ctatggtgca
      841 atgatcgtta gtttattcaa tgtggatgca ggtcaaatta tggctgttaa taatatagat
      901 ttgcacatgg gaattttatt attaattggt tttcaagcaa ttatgctatt tgcaacattg
      961 atgcagtcaa aaaaagcttt atttacaggt gaggatgcat tctatctttt tacaggtcct
     1021 tttactagaa gacaagtaat gagctattta actttccaaa caattattca ggcctttcta
     1081 cttgctttga ttagtttagt atttttagct gcatttagcg gtggtgccgg atttaatttt
     1141 atttttattg tgctagctta cttagcatct gttatcactg ttttgttttt cttattatta
     1201 acagattatc tttatgtttt atcaattggg gataagaaat atcgtaagta ttcgaaaatc
     1261 attcctggta ttattattgc ttttgttgta attatcgttt tggttttata tcttcaaacg
     1321 ggtaattatc atactttgtt tatggatttt gtgcaatcaa atttatttta tgtagttcct
     1381 atttttgggt ggatgaagtt agctttgatt gcttatgttg agcataacta tttgttagta
     1441 acattaggat atcttttgtt gtgtggagct gtaatcttag tatatgtttt atttattggt
     1501 tatcgaggta atttttatga acaggcttta caagattctc tagatttatc aaaacgaatg
     1561 aaagcggcca aagccggaga tcaggaggct ttaagaaata agaaagtaaa gcttgggatt
     1621 aagggggagt ttcgccaagg ggcttatgct gtaatgtcaa aaaatatttt attaatgcgt
     1681 aaaactaata gttttattag tgttagtgat ttgatttcaa ttggaatata tattgcagta
     1741 acaatagcag ttgacgttgg gtttggaatg tttatttata tgatggtaat ttggattttt
     1801 tcttctttac aaaattcaga t
//
LOCUS       DAOMTA010000687         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3272_length_1819_cov_2.703515, whole genome shotgun sequence.
ACCESSION   DAOMTA010000687 DAOMTA010000000
VERSION     DAOMTA010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3272_length_1819_cov_2.703515"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1449)
                     /gene="argH"
                     /locus_tag="PLK68_14515"
     CDS             complement(70..1449)
                     /gene="argH"
                     /locus_tag="PLK68_14515"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRM91662.1"
                     /translation="MNLWGGRFTKPTDQLVYDFNASISFDQTFYTQDIQGSIAHVKML
                     AKQGILTSAEGTEIETALNQILEDLNTGKLTIDNSQEDIHSFVESTLIERIGNTGKKL
                     HTGRSRNDQVALDMRLYTRDQVVILNTLIHNLLITINDIMKTHIETIMPGFTHLQKAQ
                     PITLAHHLGAYFEMFKRDSSRLEDIYKRMNICPLGSGALAGTTYPLDRGYTAELLGFD
                     GPCLNSIDGVSDRDYLIELLSAMATMMMHMSRISEEIIIWNTNEYQFVDLDDTYSTGS
                     SIMPQKKNPDIAELIRGKTGRVYGALTSLLTTMKGIPLAYNKDMQEDKELVFDALTTT
                     KGCLQLLDGMLKSTTFNKDKMRKSATGGFTNATDAADYLVNKGVPFRDAHGIIGQLVL
                     YAIDQNKDLDDLTIAEFKAISEVFDDDIYDAINVETCVNKRNTIGAPGITAMKQVIAL
                     NEKYLAQFK"
     gene            complement(1492..>1819)
                     /locus_tag="PLK68_14520"
     CDS             complement(1492..>1819)
                     /locus_tag="PLK68_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRM91663.1"
                     /translation="DRETLKYKRLVSVEFAELVYAAKWFSPLREALSAFVDKTQEVVS
                     GTTKFRLYKGNIIKEGTTSPYSLYDEDIASFATGDLYDHHDAEGFITLYGLSTKVRAM
                     KLNNKD"
BASE COUNT          576 a          321 c          277 g          645 t
ORIGIN      
        1 agataataat tttttgagac atcacttaaa aagaggggga taaaaaaata gtactagcag
       61 ctgctagtac tatttaaatt gtgctaaata tttttcattt aaggcaataa cctgtttcat
      121 tgccgttata ccaggtgcgc cgatagtatt acgcttatta acgcaagttt caacgttgat
      181 agcatcataa atatcatcat caaaaacctc tgaaatggct ttaaattcag caattgttag
      241 gtcatcaaga tctttatttt gatcaattgc atataggact agctgcccaa taattccatg
      301 agcatctcta aatgggaccc ctttattgac taaatagtct gctgcatcag tagcattagt
      361 aaatccacca gtagcagatt tacgcatttt atctttatta aatgttgttg attttaacat
      421 tccgtctaat aattgtaagc agcctttagt cgttgtgagt gcatcaaaga caagttcttt
      481 atcttcctgc atgtctttat tgtaagcaag aggaatacct ttcatcgtag ttaataaact
      541 agttaaagct ccatatactc gacctgtttt tcctctaatt aattcagcga tatcagggtt
      601 ctttttttgt ggcataattg aagagccagt tgaataagtg tcatctaaat cgacgaattg
      661 gtattcatta gtattccaaa taataatttc ttcactgatt cttgacatat gcatcatcat
      721 tgtagccata gcacttaata gttcaatcaa ataatcacga tcacttaccc catcaatact
      781 atttaagcaa ggtccatcaa aacctaatag ttcagcagta tatccgcgat ctaatgggta
      841 ggtagtacct gctagagccc cagaacctaa tgggcagata ttcattcgtt tataaatatc
      901 ttctaatcgg ctgctatcac gtttaaacat ttcaaaataa gctccgagat ggtgtgctaa
      961 tgtaattggt tgagcttttt gtaaatgtgt gaaaccaggc atgattgttt caatatgagt
     1021 tttcattata tcattaatcg ttattaatag attatgaatt aaagtattta atataacaac
     1081 ttgatcgcgt gtatataaac gcatatcgag tgcaacttgg tcattacggc ttcttccagt
     1141 atgtaacttt ttaccagtgt taccaattcg ttcaattaaa gtagattcaa cgaaactgtg
     1201 aatatcttct tgactattat caatcgttaa tttaccagta ttaaggtctt ctaatatttg
     1261 atttagagca gtttcaattt cagttccttc agcacttgtt aaaatccctt gttttgctag
     1321 cattttgaca tgagcaatac ttccttggat atcttgggta taaaaagttt gatcaaagct
     1381 aattgatgca ttgaaatcat aaactagctg atcggttggt ttagtgaatc gtccacccca
     1441 taaattcatt tcatttcctc ctgttataat aaaagggcat tagccctttt attagtcttt
     1501 attatttaat ttcattgctc ttacttttgt tgaaagtcca tataaagtaa tgaatccttc
     1561 agcatcatga tgatcatata gatcaccagt agcaaaactt gcaatatctt catcatataa
     1621 tgaataaggt gaagttgttc cttctttaat aatattccct ttatataatc tgaatttagt
     1681 agtaccacta acaacttctt gagttttatc aacaaaagca cttaaagctt cacgtaaagg
     1741 tgagaaccat ttagcagcat aaactaattc tgcaaattca acagaaacta gccgtttata
     1801 ttttaatgtt tcacgatct
//
LOCUS       DAOMTA010000688         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3274_length_1819_cov_2.270408, whole genome shotgun sequence.
ACCESSION   DAOMTA010000688 DAOMTA010000000
VERSION     DAOMTA010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3274_length_1819_cov_2.270408"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLK68_14525"
     CDS             <1..521
                     /locus_tag="PLK68_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91664.1"
                     /translation="LKNKIINKHQSTTIFNYVRISCRGDIMKKKTKNKIFSQKNIIFL
                     FIAGTLLFSCSLYYKQYLPKYLTHSIVILLIMIFCLLPFLFKRFKKQRNLIKIIVSAL
                     SFSLIVTGILVFNHNDRLAKDGQYIGIDISKWNNNVNLQLARQEIDFVIIRCGYTSLT
                     DGTKTKVDPLFE"
     gene            525..965
                     /locus_tag="PLK68_14530"
     CDS             525..965
                     /locus_tag="PLK68_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRM91665.1"
                     /translation="MKQCQNLNIPYGVYYYSLATEAAQAKKEAEYVNHLLDGRVPELG
                     VFIDLEDETFQGALTNDQLTIVATTFLDNIQNQNKKGIYANHHWWTTKLTDKKLDSYI
                     KWKARYNDTPVLEEEYHILQYSETGQIRGINGNVDLNMTINKYW"
     gene            complement(1022..>1819)
                     /locus_tag="PLK68_14535"
     CDS             complement(1022..>1819)
                     /locus_tag="PLK68_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM91666.1"
                     /translation="NKEYTVTIKSNEVTTKTIVNELIPVGKLIINKKLEGHLDDLSGI
                     EFKITAKEDIKDIITGTVLYRAGMPIGQNQGIYITDQNGQIIIDKLPMGSYLISEVKT
                     LPGYVFDDTVYEVAFNKEDRVTKEYQYTLNVENKLTQTTISKIDALTKQLLPGATLEL
                     YLKDGESLSLIEQWVSTDDAHLIKGLLVDCEYVLKEVKAPAGYTLAPELTFKVENSES
                     IKEIVFENQHIPVVPTGDISNYSGYAGIALISFIFFLIFTKQKRRIL"
BASE COUNT          619 a          292 c          239 g          669 t
ORIGIN      
        1 acttgaaaaa caaaatcatc aataagcatc aatcgacaac tatttttaat tatgttagaa
       61 ttagttgtcg aggtgatatc atgaaaaaga aaactaaaaa taaaattttt agtcaaaaaa
      121 atattatttt tctttttatt gctggtaccc ttcttttttc atgttcatta tattataaac
      181 aatatttacc aaagtatctg acccatagta tcgttatttt actaattatg atattttgtt
      241 tactgccatt tttatttaag cgatttaaaa agcaacgtaa tctaatcaaa atcatcgtat
      301 ctgctttatc atttagttta attgttactg gtatattagt ctttaatcat aatgatcgat
      361 tagctaaaga cggccaatat attggcattg atatttctaa atggaataat aatgtgaatt
      421 tgcagttagc tcgtcaagaa attgattttg tgattatccg ttgcggctat acttctctaa
      481 ctgatggtac caaaaccaaa gttgatccgc tttttgaata aaatatcaag caatgtcaaa
      541 atttaaatat tccttacggt gtatattatt attctttagc cactgaagct gctcaagcaa
      601 aaaaagaggc tgagtatgtt aatcatttac tagatggaag agttcccgaa cttggggttt
      661 ttattgattt agaagacgaa acttttcaag gggctttaac aaatgatcaa ttaacaatcg
      721 tagctacaac ttttttagat aacattcaaa atcaaaataa aaaaggaatc tatgctaatc
      781 atcactggtg gacaactaaa ttaactgata aaaaactaga ttcttacatt aaatggaaag
      841 cacgttataa tgatactcca gtattagaag aagaatatca tattttacaa tatagtgaaa
      901 ctggtcaaat tagaggaatc aatggtaacg ttgatttaaa tatgactatc aataaatatt
      961 ggtaataatt tcattttaat agcttaatta aaagccccat taactggggc tttcgtcata
     1021 tttataatat tctacgtttc tgttttgtaa aaattaaaaa gaatataaag cttattaatg
     1081 caattccagc ataaccacta taattgctta tatcaccagt tggtaccaca ggaatatgct
     1141 gattttcaaa tactatttct tttatactct cactgttctc aactttaaac gtaagttccg
     1201 gcgcaagtgt ataaccggct ggagccttga cctcttttaa aacatattca caatcaacta
     1261 acagcccctt gattaaatgt gcatcatcag ttgataccca ttgttcaatc aaactcaatg
     1321 attctccatc ctttaaatat agttctaatg ttgctcctgg taataattgc ttagttagag
     1381 cgtcaatttt agaaattgtt gtttgtgtta atttattttc tacatttaaa gtatactggt
     1441 attctttggt aaccctatct tctttattaa aagctacttc ataaacagta tcatcaaata
     1501 catatcccgg taaagtcttt acctcactaa taagataact tcccatcggt aacttatcaa
     1561 taataatttg tccattttga tctgtaatat aaattccttg attttgacca attggcatac
     1621 cagctcgata aagaactgtc cctgtaatta tatctttaat gtcctcctta gcagttatct
     1681 taaattcaat tccagataaa tcatctaaat gtccttctag tttcttattg attattaatt
     1741 taccgactgg tatcaattca ttgacaattg tttttgtagt cacttcatta cttttaatcg
     1801 taacagtgta ttccttatt
//
LOCUS       DAOMTA010000689         1809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3302_length_1809_cov_2.673318, whole genome shotgun sequence.
ACCESSION   DAOMTA010000689 DAOMTA010000000
VERSION     DAOMTA010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3302_length_1809_cov_2.673318"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>389)
                     /locus_tag="PLK68_14540"
     CDS             complement(<1..>389)
                     /locus_tag="PLK68_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRM91667.1"
                     /translation="NDGMGEVIKYGCIKDKSLFDKLNSYDNFEQLYMDIDEIIYRCIG
                     IKRDVVERDQFDFGDRLLLNFGHTLAHAIEQYYHYEKYSHGEAVAIGMVQLTKIAEEN
                     DLSPVGTSDIIKEICIKYNLPVYSGLKT"
     assembly_gap    390..427
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<428..>900)
                     /locus_tag="PLK68_14545"
     CDS             complement(<428..>900)
                     /locus_tag="PLK68_14545"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRM91668.1"
                     /translation="IEKGILNQADKYIKEIYQGNKIMIISDDQVYHYYGDLLTNVLNK
                     EYIVEHVTVPHGEQSKRFDILPSLYKALLDFKLTRTDLIIALGGGVIGDLAGFVAATF
                     LRGVKLVQIPTSLLAQVDSSVGGKVAVDLPEGKNLVGAFYHPRMVLIDPNTSKTLP"
     assembly_gap    901..951
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(985..>1809)
                     /gene="aroF"
                     /locus_tag="PLK68_14550"
     CDS             complement(985..>1809)
                     /gene="aroF"
                     /locus_tag="PLK68_14550"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM91669.1"
                     /translation="VSEPYKLANRAFHPDDTIVDVAGVKVGGDNLALIAGPCSVESEE
                     QVIEIAKAAKAAGANILRGGAFKPRTSPYAFQGMGSSGLDILVAAKEATGLPICSELM
                     DAQYLEEFNEKVDLIQIGARNMQNFDLLKKVGAGTKKPILLKRGLSATFEEWIMSAEY
                     IMANGNPNVILCERGVRTFETYTRNTLDLQAIPVVKKLTHLPIIIDPSHAGGKWWLVE
                     PMAKAAVAAGADGLMIEVHNNPEKALCDGPQSLRPERYEELLKQISKIAEVVGKKV"
BASE COUNT          491 a          385 c          242 g          602 t
ORIGIN      
        1 gtttttaatc cgctataaac tggtaaatta tatttaatac aaatttcttt tattatatca
       61 ctagtcccca ctggtgataa atcattttct tcggcaattt tagttaattg taccattcct
      121 attgcaactg cttcaccatg tgaatatttc tcataatgat aatattgttc aattgcatgt
      181 gcaagtgtat gcccaaagtt cagtaataaa cgatccccga agtcaaactg atctctttcg
      241 actacatcac gcttaatacc aatgcaacga taaattattt catcgatatc catgtataac
      301 tgttcaaagt tatcataact atttagttta tcaaacaaac ttttatcttt aatacagcca
      361 tatttgatta cttctcccat cccgtcattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnggt aatgtttttg aagtatttgg atcaatcaaa accattctcg gatgataaaa
      481 agcccccact aagttttttc cttctggtag atcaactgct actttccccc ctactgaaga
      541 atcaacttgt gctaataatg aagttgggat ttggaccaat ttaacacctc gtaaaaaggt
      601 cgctgcaaca aacccggcta aatccccaat aacaccgccg cctaaagcga tgattaaatc
      661 tgttcttgtt aatttaaaat ctaataaagc tttatataac gatggtaaaa tatcaaaacg
      721 tttagactgt tcaccatgag gaaccgttac atgctcaaca atgtattctt tatttagaac
      781 gtttgttaat aaatcaccat aataatgata aacttgatca tccgaaatta tcatgatttt
      841 attaccttgg tatatctcct tgatatattt atcagcttgg tttaagattc ctttttcaat
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcatttcc
      961 atcgttatat ttcctcctaa ttaattatac tttctttcca acaacttcag ctattttaga
     1021 aatttgtttt aataattctt catatctttc cggtcttaat gactgtggtc catcacacag
     1081 tgccttctcc ggattgttgt gcacttcgat cattaaccca tctgctcctg ctgctactgc
     1141 tgcttttgcc attggttcca ctaaccacca tttccctcct gcatggcttg gatcaattat
     1201 gattggtaaa tgtgttaatt tctttactac cggtatagcc tgtaaatcca acgtgtttct
     1261 tgtatatgtc tcaaatgttc ttacccctcg ttcacataaa attacattcg gattcccgtt
     1321 tgccatgata tattccgctg acatgatcca ttcttcaaat gtcgcactta atcctctctt
     1381 taataagatt ggtttctttg ttcctgctcc cactttcttt aacaggtcga agttttgcat
     1441 atttcttgcc ccgatctgaa ttagatctac tttctcattg aattcttcta aatactgggc
     1501 atccatcagt tccgaacaga ttggcaatcc agttgcttct ttagctgcaa caagaatatc
     1561 cagaccagaa gaccccattc cctggaatgc atacggtgaa gttcttggtt tgaaagcgcc
     1621 tcctcttaaa atattagcac cggcagcttt ggcagcttta gcaatctcga ttacttgttc
     1681 ttcactttca acagaacatg gaccagcaat caaagcaagg ttgtcacccc caactttaac
     1741 accagcaaca tcaacgatcg tatcatcagg atgaaaagcc cggttagcaa gtttatatgg
     1801 ctcagatac
//
LOCUS       DAOMTA010000690         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3306_length_1808_cov_1.962350, whole genome shotgun sequence.
ACCESSION   DAOMTA010000690 DAOMTA010000000
VERSION     DAOMTA010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_1808_cov_1.962350"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLK68_14555"
     CDS             complement(<1..315)
                     /locus_tag="PLK68_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM91670.1"
                     /translation="MENKGIIFNIQKFSIHDGPGIRTTIFFKGCPLRCKWCSNPESQL
                     KAVQILWDHNKCTHCMTCINNCPTGAIKLIADKIIIDQNKCNGCLRCVNKCPQIALKN
                     EGE"
     gene            527..>1808
                     /locus_tag="PLK68_14560"
     CDS             527..>1808
                     /locus_tag="PLK68_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM91671.1"
                     /translation="MKNIEHFGDLTPRMNDFREKVLDKKPYICAERALLATESYRLYQ
                     NQPPVMKRALMLKNILEKMSIYIEDETLIVGNQAASNKDAPIFPEYTLEFVIDELDKF
                     EKRDGDVFYITEETKAALRSIAPFWENNNLRAKGEALLPEEVNVFMETGFFGMEGKLN
                     SGDAHLAVDYEQLLKIGLVGYEKRVRQLKAELDLCVPENIDKYVFYKAVLIVIEAVKT
                     YADRFSLLAQEMAENAQSHRKDELLEISNICSKVPYEPASSFKEAIQSVWFIQLILQI
                     ESNGHSLSYGRFDQYMYPYLKADLEKGVITDEEAVELLTNLWIKTLTINKVRSQAHTF
                     SSAGSPMYQNVTIGGQTPDKKDAVNKLSFLVLKSVAQTRLPQPNLTVRYYNGLNKEFL
                     DECIEVMKLGTGMPAFNNDEIIIPSFIDLGVKEED"
BASE COUNT          640 a          225 c          319 g          624 t
ORIGIN      
        1 ctcaccttca ttttttaggg caatttgagg acatttatta acacatctca aacatccatt
       61 acatttattt tgatcaataa taattttatc tgcaattaat ttaatcgcac ctgtaggaca
      121 attattgata caggtcatgc aatgcgtaca tttgttatga tcccacaaaa tttgaactgc
      181 ttttagttgt gattcaggat tagaacacca cttacatctt aatggacagc ctttaaaaaa
      241 tatagttgtt ctaattcctg gaccatcatg aatactaaat ttttgaatat taaaaattat
      301 ccccttattt tccattatga atatctcctt atcttttcat tatttataat atcatgttga
      361 ttgcaaattg taaatgttta tatttcattt gaaaccaata tatactgctt ttgaaataaa
      421 aatattgact tattgttcgt atatgttaat atataggtgt aaatgaagtg aatttatgtt
      481 atgaacgaaa cttaataatt tattttagta actttggagg aaaaatatga aaaacattga
      541 acattttggt gatttaacac ctagaatgaa tgattttaga gaaaaggttt tagataaaaa
      601 accgtatatt tgtgcagaga gagcattatt agctacagaa tcttatagat tatatcaaaa
      661 tcagccaccg gttatgaaac gggcactaat gttaaaaaat attttagaga aaatgtcaat
      721 ttatattgaa gatgaaactt taatagttgg gaatcaggct gcttcaaata aagatgctcc
      781 aatttttccc gaatatacgt tagaatttgt tattgatgaa ttggacaaat ttgaaaaaag
      841 agatggtgat gttttttata ttactgaaga aacaaaagca gctttacgtt caattgcacc
      901 attttgggag aataataatt taagagctaa gggggaagct ttactacctg aagaagtaaa
      961 tgtttttatg gaaactggtt tctttggaat ggaaggaaaa ttaaattctg gagatgctca
     1021 tcttgcggtt gattatgaac agttactaaa aataggatta gtcggttatg aaaaaagagt
     1081 gcgacaatta aaagcagagt tggatctatg tgttccggaa aatattgata aatatgtttt
     1141 ttataaagct gtattgattg taattgaagc agttaaaact tatgctgata ggtttagtct
     1201 tcttgctcaa gaaatggctg aaaatgctca aagtcataga aaggatgagt tattagaaat
     1261 tagtaatatt tgttctaaag tgccttatga accagcttca tcttttaaag aagcgattca
     1321 atcagtttgg tttattcaat tgattttgca aattgaatct aatggacatt cattatcata
     1381 tggacgtttc gatcaatata tgtatccata tttgaaagcg gatctagaaa aaggcgtaat
     1441 cacggatgag gaagctgttg agttgctaac aaatttatgg atcaaaacat taactattaa
     1501 taaggtcaga agccaggctc acacatttag cagtgctggt agtccaatgt accaaaatgt
     1561 aactattggt ggacaaactc ctgataaaaa agatgcagtg aacaaacttt cgtttttagt
     1621 tttgaaatct gttgctcaaa caagattgcc acagcctaat ttgactgtta gatattataa
     1681 tggtttaaat aaagaatttt tagatgaatg tatcgaagtt atgaagttag gaacaggaat
     1741 gccggcattt aataatgatg aaattattat tccttcgttt attgatctgg gagtaaaaga
     1801 ggaagatg
//
LOCUS       DAOMTA010000691         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3320_length_1805_cov_2.081714, whole genome shotgun sequence.
ACCESSION   DAOMTA010000691 DAOMTA010000000
VERSION     DAOMTA010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_1805_cov_2.081714"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PLK68_14565"
     CDS             <1..1707
                     /locus_tag="PLK68_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRM91672.1"
                     /translation="PTKKDIKMHQSASKFTDFFTPKKDKAKGFFPEEIFGDENTSSQE
                     QDPPGKSSARIHTTHLEEDESVPSTFEFDEDSMTLEIKDKEPKIHENDMSKPKKKINK
                     NYRLPALSLLKNPTAKKSGDNKGNALSKAEALTNVLHEFGVNATISDIFIGPSVTKYE
                     LKLETGTRVNKIMQLQDDIKLALAAKDIRIEAPIPGKPAVGVEIPNSVATMVSFKEVI
                     KDIPKDLQDNKLLVPLGKDVSGKIIYAELNKMPHLLIAGATGSGKSVCVNTIICSILM
                     RARPDEVKFILVDPKKVELTNYNGIPHLLAPVVTDPKKAAAVLQEVVVEMEHRYDLFA
                     GANVRNIEGYNNYARKKNEELALDEQLEILPFHVVILDEVADLMMVASKQVEDCIMRI
                     AQMARAAGIHLIVATQRPSTDIITGVIKANIPSRIAFAVSSGIDSRTMLFSPMGSSSP
                     VRVQGAFVSDDEVSAITHHTATQQEASYDDKYINVKLNTTSPSAASKEEEEDEEYEMC
                     RSFVINAQKASTSLLQRQFRIGYNKAARIIDQLEADGVIGPQIGSKPREVYIRGYQEE
                     DI"
BASE COUNT          642 a          277 c          357 g          529 t
ORIGIN      
        1 cccactaaaa aagatattaa aatgcatcaa agcgctagta aattcacaga tttttttacc
       61 cctaaaaaag ataaagcaaa aggctttttt ccagaggaga tatttggtga tgaaaatact
      121 tcaagtcaag agcaagatcc ccctggaaag agttcagctc gaatccatac tactcattta
      181 gaagaagatg aatcagtacc ctctacattt gaatttgatg aggattctat gactttagaa
      241 attaaagata aagaaccaaa gatccatgaa aatgatatgt ctaaacctaa gaaaaagatt
      301 aataagaatt atcgtttacc tgccttatcc ctattaaaaa atcctacagc aaaaaagtct
      361 ggtgataata aaggaaatgc attatctaaa gctgaagcac taacaaatgt tcttcatgaa
      421 tttggagtaa acgcaacaat tagtgatatt tttattggac catcggttac taaatatgaa
      481 ttaaaattgg aaacgggcac gcgtgttaat aaaattatgc aattacaaga tgatataaaa
      541 ttagccttag ctgcaaaaga tattcgtatt gaagcaccaa ttcctggtaa accggcagtt
      601 ggagtggaaa ttcctaatag tgttgcaaca atggtaagtt tcaaagaagt aattaaagac
      661 attccaaaag atttacagga taacaagttg ttagttcctt taggcaagga tgttagtggt
      721 aagattattt atgcggaatt aaataaaatg ccccacttat tgattgctgg ggcaactgga
      781 agtggaaaat cagtttgtgt aaatactatt atttgtagta ttttgatgcg tgctcgaccg
      841 gatgaggtga aatttattct tgtcgatcct aaaaaagtag agttaactaa ttataatggt
      901 atacctcatt tattagctcc agtagtaact gatcctaaaa aggcagctgc agtattgcag
      961 gaagttgtag tggaaatgga gcatcgctat gacctttttg ctggggcaaa tgtacggaat
     1021 atcgaaggct ataataacta tgcacgtaaa aaaaatgaag aattagcttt ggacgaacaa
     1081 ttagagatct taccttttca tgtagttatt ttagatgagg ttgctgattt gatgatggtc
     1141 gcaagcaaac aagttgaaga ttgtatcatg aggattgctc aaatggcgcg ggcagcaggg
     1201 attcatttga tcgttgcaac acaaagacca tcgactgata ttatcactgg agtcatcaaa
     1261 gcaaatattc catcaagaat tgcttttgca gtctcttctg gaattgattc acgaacaatg
     1321 ttattttcac caatgggttc tagttcaccg gtgcgggttc aaggtgcttt tgtatctgac
     1381 gatgaagtta gtgcaattac tcaccacact gctactcagc aggaggctag ctatgatgac
     1441 aaatacataa atgtaaaatt aaatactact tcaccaagtg ctgcttcgaa agaagaagag
     1501 gaagatgaag aatatgaaat gtgtcgcagt tttgtaatca atgctcaaaa agcaagtaca
     1561 tctttattac aacggcagtt tcgaattgga tacaataaag cagcacgaat catcgatcag
     1621 ttagaagcag acggtgttat tggaccacag attggctcta agccacgtga agtttacatt
     1681 cgcggatatc aagaagagga catctaaata gatgtctttt tttgtatatt ttataaaaaa
     1741 acgggaataa taaatgtaat cgctttcaat ttttaaggaa gaatgttaaa ataaagctat
     1801 agttt
//
LOCUS       DAOMTA010000692         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3321_length_1805_cov_2.055429, whole genome shotgun sequence.
ACCESSION   DAOMTA010000692 DAOMTA010000000
VERSION     DAOMTA010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3321_length_1805_cov_2.055429"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLK68_14570"
     CDS             <1..826
                     /locus_tag="PLK68_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91673.1"
                     /translation="RINEARPILTDTEIEIADFILEHKLDVINDSAQSLAQKTMTSPA
                     AVIRFSKKVGFSGFSQLKIELAKNLANDSMEFDNLLDPYEHMESLMKKAYRSNIQTIE
                     KTYGLIDSTVIERVAQEIISCRNIYLFGIGSSGIVCEDFQHKLLRIGKTSIYYTDTHL
                     QLTAVPNMQKDDLAFFVSYSGKTKEIVTAAKWAKRVGIKSVAITQSAYNDLGKLVDMV
                     ITIPIEEKELRIGATSSRLSSLVVIDLLYYGVARHDKEETHKSIVDTRKIIDEIQK"
     gene            complement(985..>1805)
                     /locus_tag="PLK68_14575"
     CDS             complement(985..>1805)
                     /locus_tag="PLK68_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRM91674.1"
                     /translation="NKGSLYRSTLQIGEAAILAYQFQTTVVSNFRVMDMAAGGQGAPL
                     VPYSEYILYSQPHKNRVLQNIGGIGNLTFLNGSLNIDDIFAFDTGPGNMMINEACNIL
                     YDLPFDHGQIAKQGKIISALLDELMNHPYMKKLPPKSTGREDFGEFFVKDILARYSQH
                     KRADIITTFTMFTAKSIAFHYQRDILSKYSLDDVIIGGGGSHNDTLMQFIKDELYPIP
                     VYTQDEIGYSSDAKEAVAFAILGNETLNHHYSNIKSSTGANRYVILGQITPKPF"
BASE COUNT          620 a          274 c          328 g          583 t
ORIGIN      
        1 tagaataaat gaggcaagac ctatactaac agatactgaa attgaaattg cggattttat
       61 attagagcat aaattagatg ttattaatga ttcggctcag tcattagctc aaaaaacaat
      121 gacatcaccg gctgctgtta ttcgtttttc aaaaaaagta gggttttctg gtttttcaca
      181 actaaaaata gaattggcta aaaatttagc taatgattct atggaatttg acaatttatt
      241 agacccttat gagcacatgg aatcattgat gaaaaaagca taccggtcaa atattcaaac
      301 aattgaaaag acttatggat taattgattc aacggttatt gagagagtgg ctcaggagat
      361 tatttcgtgt cgcaacatat atttgtttgg gattggtagt tcaggtatag tgtgtgagga
      421 ttttcagcat aaacttttaa gaataggtaa aacatcaatt tattataccg atacacatct
      481 tcagttaact gcagtaccaa atatgcagaa agacgattta gcattctttg tcagttacag
      541 tggaaaaaca aaggaaatag ttactgctgc aaaatgggct aagcgtgtgg ggattaaatc
      601 tgttgctata acacaatcag cttataatga tttgggaaaa ttagttgata tggttattac
      661 gattccaatt gaagaaaaag agttaagaat tggcgcgaca tcatcaaggt tatcttcttt
      721 ggttgttatt gatttactat attatggagt tgctagacat gataaagaag aaacgcataa
      781 gagtatagtt gatacaagaa agattattga tgaaatacag aaatagaaat aatgtcctga
      841 acccttaaag ttataccaac tataggaggt tcactacatt atttctattt ttttaaatac
      901 gcgctattga agtgaatact ggatgctgga taacatctcc agatttactt caatagcacg
      961 tgaatgtact gtaaaaaaat attttcaaaa tggcttagga gtgatttgtc ccaggataac
     1021 ataacgattt gctccagttg aggatttaat attagaataa tgatgattca atgtttcatt
     1081 tcctaatata gcaaaagcaa cagcttcctt ggcatcactt gaatagccaa tctcatcttg
     1141 agtataaaca gggattggat ataactcatc tttgataaat tgcattaatg tatcattatg
     1201 gcttccgcca ccgccaataa taacatcatc caaagagtac tttgagagaa tgtctctttg
     1261 ataatgaaat gcaatgcttt ttgctgtgaa cattgtaaaa gttgtaataa tatcggccct
     1321 tttatgctga gagtatcttg ctaaaatatc tttaacaaag aactccccaa aatcttctcg
     1381 accagttgat ttgggtggga gttttttcat ataggggtga ttcattaatt cgtcaagtag
     1441 tgcggagata attttacctt gttttgcaat ctgtccatga tcaaatggta aatcatagag
     1501 gatgttacaa gcctcattga tcatcatatt cccaggtcca gtatcgaaag caaaaatatc
     1561 atcaatattt aaagatccat ttaaaaatgt taaattacca atacctccaa tattttgaag
     1621 gactctattt ttatgtggtt gactataaag aatatattcg ctatacggta ctaatggggc
     1681 gccttggcca cctgcggcca tatccataac cctaaaatta gatacaacag ttgtttgaaa
     1741 ctgataagca agaattgctg cttcaccaat ctgtagagtt gaacggtata aagaaccttt
     1801 attct
//
LOCUS       DAOMTA010000693         1799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3337_length_1799_cov_2.213303, whole genome shotgun sequence.
ACCESSION   DAOMTA010000693 DAOMTA010000000
VERSION     DAOMTA010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_1799_cov_2.213303"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLK68_14580"
     CDS             <1..906
                     /locus_tag="PLK68_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRM91675.1"
                     /translation="YSTTAHIIVEMKVPKDLTSRKQKKCDTSLKIEFQIELKQGERNL
                     DITTNIDNTAKDHRLRVLLPTDIWAQASVSDNQFGIIERPVKDMAIESWQEDNWSERP
                     DSIYPMLSYVAIKGCPLALLTNSVREYEVIGDNYNTLAITLFRSVGYLGKEELVRRPG
                     RPSGIKMPTPDSQLLKPLEFRFALVIENDHAKLSRLAKDYLTPLISYNKMPYNAMKLN
                     EVDFNAPYTFSLLHENNELTTLSVLKKAESSNKLIYRTFNTNNKEVLISLNHDLKDFV
                     DLKEEIVKTNNKLKKNQVKSFILDD"
     gene            1011..>1799
                     /locus_tag="PLK68_14585"
     CDS             1011..>1799
                     /locus_tag="PLK68_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl/aryl-sulfatase"
                     /protein_id="HRM91676.1"
                     /translation="MLGEEKLINFTKTNYQKKITKVNERVYHFLGYGHSNAIAIIGET
                     SIILIDTLDSDYRAKVMKDELAKISDKPVKTIIYTHGHPDHLGGAGAFRDTVVEVIAF
                     KPRKPILKYYDRISKLLNQRGIYQHGYGLTDQEAISQGIGIREGKEIKEGKYDFIKPT
                     TYYEQKVVERVIDGIKLKMVSAVGEADDEIYVWLEDDQIMCCGDNYYGCWPNLYAIRG
                     TMYRDVATWIDTLNEILNYPAVALLPGHTRVLWQNEIKAIVGTFR"
BASE COUNT          681 a          230 c          340 g          548 t
ORIGIN      
        1 tattctacta cagctcatat aattgtcgaa atgaaagtac caaaagattt aacaagtcgt
       61 aaacaaaaga aatgtgatac aagtttaaaa attgaatttc aaatagaatt aaaacaaggg
      121 gaacgaaatc ttgacattac aactaatatt gataatacag ctaaagatca tcgacttaga
      181 gtacttcttc caactgatat ttgggctcag gcctcagtta gtgataatca gtttgggata
      241 attgaacgcc cagtaaaaga tatggcgata gaatcatggc aagaagataa ttggagcgag
      301 cggccagata gtatttatcc aatgcttagt tacgttgcaa tcaagggttg tccattagct
      361 ttactcacta attcagttag agaatatgaa gtaattgggg ataattataa taccctggca
      421 attacgttat ttcgatcagt tggctattta ggtaaagaag aattagttcg gcgtccggga
      481 cgaccatcag gaattaagat gcctacacca gattcacaat tattaaaacc attagagttt
      541 agatttgcgc ttgttattga aaatgatcac gctaaattat cacgtttagc taaagattat
      601 ttaacgccat taattagtta taataaaatg ccgtacaatg cgatgaagtt aaatgaggtt
      661 gattttaacg ctccatatac tttctctctg cttcatgaaa ataatgaact tactacacta
      721 agtgttttaa aaaaggcaga atcttcaaat aaattaattt atcgaacttt taatactaat
      781 aataaagagg ttttaatttc tttaaatcat gatcttaaag attttgttga tttaaaagaa
      841 gagatagtta agactaataa taaactaaaa aagaatcaag taaagtcatt tattttagat
      901 gattaaataa aaccgtagtc caaatgacta cggttttgac agttacctaa aggtgcctac
      961 tattaaaaga ctagataatc aatataatga tatataagga ggaataagca atgttaggtg
     1021 aagaaaagtt aattaatttt acgaaaacaa actatcaaaa gaaaattact aaagttaatg
     1081 aacgggtata tcattttttg ggctatggac atagtaatgc aattgcgatt attggggaaa
     1141 cttcaattat attaattgat actcttgatt ctgattatcg tgcaaaagtg atgaaagatg
     1201 aactagcgaa aattagtgat aaaccggtta aaacaattat ttatactcat ggacatcctg
     1261 atcatttagg aggagcaggt gcttttcggg atacagttgt agaagtaata gcatttaaac
     1321 ctcgaaaacc aattttaaaa tactacgata gaattagtaa actactaaat caacgaggga
     1381 tttatcagca tggttatgga ttaacagatc aggaagcgat tagtcaaggc attggaatcc
     1441 gtgaaggaaa agaaattaaa gaaggaaaat atgattttat aaaaccgacg acatattatg
     1501 agcaaaaagt ggttgaacga gtaattgacg gaattaaatt aaagatggtt agtgctgtag
     1561 gtgaagcaga tgatgaaatc tatgtttggt tagaagatga tcagataatg tgttgtggag
     1621 ataattatta tggatgctgg cctaatcttt atgcaataag aggaaccatg tatcgcgatg
     1681 ttgcaacatg gattgatact ttaaatgaga ttttaaatta tccagctgtt gcattattac
     1741 caggacatac acgagtttta tggcaaaatg agattaaagc aatagtggga acttttagg
//
LOCUS       DAOMTA010000694         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3349_length_1795_cov_2.620690, whole genome shotgun sequence.
ACCESSION   DAOMTA010000694 DAOMTA010000000
VERSION     DAOMTA010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_1795_cov_2.620690"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1208
                     /locus_tag="PLK68_14590"
     CDS             3..1208
                     /locus_tag="PLK68_14590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRM91677.1"
                     /translation="MSGRDIFGCAKTGTGKTAAFALPILQKLYLRDESEKYPRTIKAL
                     ILAPTRELAIQINETFEAMNPQVNLKSAVIFGGVRQGSQVTKINRGIDVLIATPGRLI
                     DLYNQGLVDLKHVEYLVLDEADRMLDMGFIKDIRKILRFIPRRHQTMLFSATLPDEIK
                     HLVSDLLNDPLKIMISSGNVTVEKINQSLYFVDKVNKAKLLIKLLENPQIYNAIVFVR
                     TKRNVDTLCKKLIKAQITCEGIHGDKSQNARVRALNNFKNDKVRVLVASDIAARGIDI
                     DELTHVINFDLPDQAENYVHRIGRTARAGASGEAITFCSFQEKALLKDIQKFINQDIP
                     VVDNPYYPMKDLSIPEKKHKSKKKGETQKSRDKAKHLKQGQKKSSVRTNNIKKNSKTK
                     RSNFKRRRP"
     gene            1309..>1795
                     /gene="tilS"
                     /locus_tag="PLK68_14595"
     CDS             1309..>1795
                     /gene="tilS"
                     /locus_tag="PLK68_14595"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRM91678.1"
                     /translation="MDSFKDLLKMQELYILGVSGGCDSMALLDMMNQAGYQIIVCHVN
                     YHLREDSDLDQQTVEAYCHRYDLPCYVREIDKQVYGPDNFQDQARRLRYQFYLEIGMK
                     YQTQKVVLAHHQNDVIENIVMQLQRHNTKGYLGIQEISEVFGVTVIRPCLAVRKQFLR
                     DY"
BASE COUNT          654 a          250 c          356 g          535 t
ORIGIN      
        1 ttatttcggg aagagatatt tttggatgtg ccaagactgg aacagggaaa acagcagctt
       61 ttgctttacc gattttacaa aaattatatt tacgtgatga aagcgaaaag tatccacgaa
      121 ctattaaagc actgattttg gctccaactc gtgaattagc aattcaaatc aatgagactt
      181 ttgaggcaat gaatccgcag gttaatttaa aatcagcagt tatctttggt ggggttcgtc
      241 agggtagtca ggttactaaa attaatcgtg ggatcgacgt gctgattgcc acgccaggaa
      301 gactgattga tctttataat caaggattgg tggatttaaa acatgtagaa tacttagttt
      361 tagatgaagc agatcgtatg ttggatatgg gatttattaa agatattcgt aagatcttaa
      421 gatttattcc tcgtcgtcat caaactatgt tattttcagc aaccttacct gatgaaatta
      481 aacacctggt tagtgatcta ctgaatgacc cgcttaaaat catgataagc agtggtaatg
      541 ttacagtaga aaaaattaat caatcgctat actttgttga taaagtaaat aaagctaagt
      601 tattgatcaa gttattagaa aatcctcaaa tatataatgc aattgttttt gttaggacaa
      661 aaagaaatgt ggatacttta tgtaagaaac taattaaggc gcaaataact tgtgagggaa
      721 tccatggtga taaatctcaa aatgcacgtg ttcgagcatt aaataacttc aaaaatgata
      781 aagttcgagt tcttgttgct agtgatattg ctgctcgagg gatcgatatt gatgaattaa
      841 cacatgttat taattttgat ttacctgatc aagcagaaaa ttatgttcat cggatcggcc
      901 gaactgctcg tgctggagca tcgggagaag caattacatt ttgtagtttt caagaaaagg
      961 cattattaaa agatattcaa aaatttatta accaagatat tccggtggtg gataaccctt
     1021 attacccaat gaaagattta tcaataccag aaaagaaaca taaaagtaaa aaaaagggtg
     1081 aaacacaaaa atcacgtgat aaagctaagc acttgaaaca aggacaaaaa aagtcatcag
     1141 taagaacaaa taacataaaa aagaatagta agacaaaaag aagtaatttt aaaagaaggc
     1201 gtccttaaaa atgacgtctt ttactaattg attagtgaaa aagtcacgaa aatgatatgt
     1261 taaattaata attatttgat ataatggtaa agatattgag gtgattaaat ggatagtttt
     1321 aaagatttgc tgaaaatgca ggagctgtat atccttgggg tatcaggggg ctgtgattcg
     1381 atggcattat tagacatgat gaatcaagct ggttaccaaa taatcgtatg tcacgtcaat
     1441 tatcatttac gcgaagatag tgacctagat caacagacag ttgaagcata ttgtcaccga
     1501 tatgatttgc cttgttatgt tcgagaaatt gataaacaag tttacggacc ggataatttc
     1561 caagatcaag ctcggcggct acgctatcaa ttttatttag aaattggaat gaaatatcaa
     1621 acccaaaaag tggttttggc ccaccatcaa aatgatgtta ttgaaaatat tgtaatgcag
     1681 ttacagcgcc ataacactaa gggatatctg ggaatccaag aaatcagtga agtatttgga
     1741 gtaactgtaa ttagaccgtg cttagcggta cgaaaacagt ttttaagaga ctatt
//
LOCUS       DAOMTA010000695         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3350_length_1795_cov_2.350575, whole genome shotgun sequence.
ACCESSION   DAOMTA010000695 DAOMTA010000000
VERSION     DAOMTA010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_1795_cov_2.350575"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLK68_14600"
     CDS             complement(<1..348)
                     /locus_tag="PLK68_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM91679.1"
                     /translation="MILKENYKLNNGVEIPKLGLGTWEIADNKVADAVKKAVKLGYRH
                     IDTAQGYENENGVGIGIKTCGVERENLFITTKIQADFKTYEEARRSIIDSLKRLELEY
                     IDLMIIHAPQPWTK"
     gene            complement(362..616)
                     /locus_tag="PLK68_14605"
     CDS             complement(362..616)
                     /locus_tag="PLK68_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91680.1"
                     /translation="MVKLNVQKISEKEIVIALLDAGMDTEKINEFIESINSEYFQKSK
                     NILLSQRAELLLKIRDGQEKLYCLDYLTRTLNQAGIFDKK"
     gene            complement(784..>1795)
                     /locus_tag="PLK68_14610"
     CDS             complement(784..>1795)
                     /locus_tag="PLK68_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91681.1"
                     /translation="DTPLYLIEKYGGWSNRKLIKFFDHYARTVFTEFKGLVKYWLTFN
                     EINNTIMGLGLFYEAKEEDYQRAYQHLHHQFVASAHAVKIGHEIDSENKIGCMICGIT
                     SYPATCDPKDVLANRHQWEKSIFYCGDVQCFGKYPTYAKRLWDEHNVKLDITEQDLID
                     LKEGTVDMYTFSYYMSSLITTHEIADAVGGNFTTGARNEYLQYSDWGWAFDPDGLQYY
                     LEMIYDRYQRPLMVVENGLGAFDTVEEDGTIHDDYRIDYYRDHILAMDKAINNGVDLI
                     AYTTWGCIDLVSAGTGEMRKRYGFIYVDKHDDGTGTMKRSPKDSFYWYQKVIQSNGQD
                     LD"
BASE COUNT          588 a          357 c          193 g          657 t
ORIGIN      
        1 ttttgtccat ggttgtggtg catgaatgat cattaaatca atatattcaa gctctaatct
       61 ctttaatgaa tcaataatag atcgtctcgc ttcttcatat gttttaaaat ctgcttgaat
      121 ctttgttgtt ataaaaagat tctctcgctc aacaccacaa gttttaattc caattccaac
      181 accattttca ttctcatatc cttgggcagt atcgatatgg cgatacccta gtttaacagc
      241 tttctttact gcatcagcta ctttattatc agcaatttcc caagttccta accctaattt
      301 aggtatttct accccattat ttaatttata attttccttt aagatcataa tttttcctcc
      361 attatttttt atcaaatatt cctgcctgat tgagtgttct agttaaataa tctaaacaat
      421 ataatttctc ttgaccatct cgaatcttta ataataattc agctctttgg cttaataaaa
      481 tatttttact tttttgaaaa tattctgagt taatactttc aataaattca ttgatttttt
      541 ccgtatccat accagcatcg agcagtgcta taacaatttc tttttcacta attttctgca
      601 cattcaattt aaccatctcc tttcaagccg ttttattcta cacttataat tatcaataaa
      661 agccatgaaa atacaaatac ttatatttta tggttttcca tgctttttat ttatgcaaaa
      721 aactgtatcg tcatctttat atgacaatac agttaatcat tacttataaa attaaatact
      781 caattaatct aaatcttgac cattactttg tattactttt tgataccaat agaaagaatc
      841 tttaggacta cgtttcatcg ttccagttcc atcatcatgt ttatcaacat agataaaacc
      901 ataacgtttt ctcatttcac cagtgcctgc cgaaactaga tcgatacagc cccaagtcgt
      961 atatgcaatt aaatcaacac cattattaat tgctttatcc atagccaaga tatgatctcg
     1021 ataataatcg atacgataat catcatggat tgttccatct tcctcaactg tatcaaaagc
     1081 tcctaaacca ttttctacaa ccatcaatgg cctttgataa cgatcataaa tcatttcaag
     1141 ataatattgt aatccatctg gatcaaacgc ccatccccaa tcagaatatt gaagatattc
     1201 atttcgtgct cctgttgtaa agtttccacc tactgcatca gcaatctcat gagttgtaat
     1261 caaagaagac atataataag agaaagtata catatcaaca gtaccttctt ttaaatcgat
     1321 taaatcttgt tctgtaatat ctaatttaac attatgttca tcccataaac gtttagcata
     1381 agtcggatat ttaccaaaac attggacatc accgcaatag aagattgatt tttcccattg
     1441 atgacgattt gctaaaacat ccttaggatc acatgttgct ggataacttg taataccgca
     1501 aatcatacaa ccaatcttat tttcggaatc aatttcatga cctatcttaa ccgcatgagc
     1561 acttgcaaca aattgatgat gtaaatgttg ataagcacgt tgataatcct cttctttagc
     1621 ctcatagaat aaacctaatc ccatgattgt attattaatt tcattaaaag ttaaccaata
     1681 tttaactaaa cctttaaact ctgtaaaaac tgttcgtgca taatgatcaa aaaacttgat
     1741 caactttcta tttgaccatc ctccatattt ttcaattaaa tataaaggtg tatca
//
LOCUS       DAOMTA010000696         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3357_length_1794_cov_1.957447, whole genome shotgun sequence.
ACCESSION   DAOMTA010000696 DAOMTA010000000
VERSION     DAOMTA010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_1794_cov_1.957447"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /gene="valS"
                     /locus_tag="PLK68_14615"
     CDS             <1..1721
                     /gene="valS"
                     /locus_tag="PLK68_14615"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRM91682.1"
                     /translation="TMNELAHKYAGMDRFACREALVADFKAAGVVDHIEKHMHQVGHS
                     ERSNAIVEPYLSKQWFVKMEPLAKAALENQLKDSKVNFVPERFEKTFNQWMENIEDWC
                     ISRQLWWGHQVPAWYHKETGEVYVGKNPPADLENWKQDEDVLDTWFSSALWPFSTLGW
                     PNTDSELFKRYFPTNTLVTGYDIIFFWVSRMIFQSLHFTEERPFEHVLIHGLIRDEQG
                     RKMSKSLGNGVDPMDVIDEYGADTLRFFLTTNSAPGMDLRYIPEKLEASWNFINKIWN
                     SARFVLMNIDDEMKFEELSFDNLNLCDKWILNRLNEVIREVDINMDKFEFVNVGSELY
                     KFIWDDFCSWYIELTKVHLNSTNDTEKQASLNTLVYVLNAIVKMLHPFMPFVTEEIFQ
                     AIPHLEESICIAIWPEVNDHFTDESINDQFTYLIDIVKGIREIRTQYTIKNAIEVPYV
                     INTKNDDLEGLLNKCLPYIKKLCNAVCSGYNLNAAGEVANITIKGGNSLLVELGDYID
                     KDAEKEKLANQLKKLEGEIKRCQNMLANEKFTSKAPKEKVELERNKLADYQSKYDAVK
                     EKLEQM"
BASE COUNT          647 a          218 c          346 g          583 t
ORIGIN      
        1 gaactatgaa tgaactggca cataaatatg caggaatgga tcgttttgct tgtcgagagg
       61 ctttggttgc tgattttaaa gcagctgggg ttgttgacca tattgaaaaa catatgcatc
      121 aggtggggca ttcagaaaga agtaatgcaa tcgttgaacc atatttatca aaacaatggt
      181 ttgttaaaat ggagccatta gctaaggcag ctttagaaaa tcagttaaag gattctaaag
      241 taaattttgt ccctgaacgt tttgaaaaaa catttaatca gtggatggaa aatattgaag
      301 actggtgtat atctcgtcaa ttatggtggg gacatcaagt accggcttgg tatcataaag
      361 aaaccggtga agtttatgtt gggaaaaatc cacctgctga tttagaaaac tggaaacaag
      421 acgaagatgt cctagataca tggttttcaa gtgcactatg gccattttca acacttggat
      481 ggcctaatac cgatagtgaa ttatttaagc gttattttcc taccaatact ttagttacag
      541 ggtacgatat tattttcttc tgggtgagca gaatgatttt ccaatcatta cacttcactg
      601 aggaaagacc attcgaacat gttttaattc atggtttgat tcgtgatgaa caagggagaa
      661 agatgtcaaa atcgttaggc aatggtgttg atccgatgga tgttattgat gaatatggtg
      721 ctgatacatt acgcttcttc ttaacaacta attcggcacc aggaatggat ttgcgttata
      781 ttccggaaaa attagaagca tcatggaact ttatcaataa aatttggaat agtgctcgtt
      841 ttgttttaat gaatattgat gatgaaatga aatttgaaga attatcattt gataatctaa
      901 atctttgtga taaatggatt ttaaatcgtt taaatgaagt gattcgtgaa gtagatatta
      961 acatggataa atttgaattt gttaatgttg gtagtgagtt atataaattt atttgggatg
     1021 acttctgttc atggtatatt gaacttacaa aagtacattt aaatagtact aatgatacag
     1081 aaaaacaggc aagtttaaat acattagttt atgttttaaa tgcgattgtt aaaatgctgc
     1141 atccattcat gccatttgta actgaagaaa tcttccaggc aattccacat ttagaagaat
     1201 ctatttgtat tgctatctgg ccagaagtaa atgatcattt tactgatgaa agtattaatg
     1261 atcaatttac atatttaatc gatattgtaa aaggtatccg tgaaatcaga acgcaatata
     1321 cgattaaaaa tgcaattgaa gtgccatacg tgatcaatac taaaaacgat gatctagaag
     1381 gattgttaaa taaatgttta ccttacatca agaaattatg taatgcagtt tgttcaggtt
     1441 ataatcttaa tgctgctggt gaggttgcaa atattacaat taaaggtggc aattcattat
     1501 tagttgaatt aggggattat attgataaag atgctgaaaa agaaaaatta gctaatcaac
     1561 ttaaaaagtt agaaggagaa attaaacgtt gtcaaaatat gttggcaaat gagaagttca
     1621 cttcaaaagc tccaaaagaa aaagttgaac tagaaagaaa taaattagcg gattatcaat
     1681 ctaagtatga tgctgtaaaa gaaaaattag agcaaatgta aaaagatgaa gaaattcatc
     1741 ttttttctat ttttaaaagt aaaaattatt ttcttgcagt aaataataat ataa
//
LOCUS       DAOMTA010000697         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3380_length_1787_cov_2.271363, whole genome shotgun sequence.
ACCESSION   DAOMTA010000697 DAOMTA010000000
VERSION     DAOMTA010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3380_length_1787_cov_2.271363"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..820
                     /locus_tag="PLK68_14620"
     CDS             98..820
                     /locus_tag="PLK68_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91683.1"
                     /translation="MLEIKHYTKIYSDNKKAVDDLNITVKSGEIHAFIGHNGAGKTTT
                     IRAIVGVMDFEQGEILIDSHSIKKEPVLCKSLTAYIPDNPDLYEYITGIQYLNYICDM
                     FGVSEIERTSGIQKYAELFKITDSLGDLISSYSHGMKQKLALIGAFIHKPKLFVFDEP
                     FVGLDPEASFNLKSIMKEACQNGSAIFFSTHVLEVAEKLCDTVSIINSGKLIASGTME
                     EIKGQHSLEDVFMEVVENGKEI"
     gene            804..>1787
                     /locus_tag="PLK68_14625"
     CDS             804..>1787
                     /locus_tag="PLK68_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91684.1"
                     /translation="MAKKYKLLLKMQLYSLFGLNRLLHSHDKKEKNRTLVIGIAGLAI
                     IGILVYLSGYICSVFADAGLVEAIPTLIIVIYSLIVLLLTFLKSSGALVGLKDYDMVM
                     SLPVNNTSVVLSRLTMLYIMSLVIGMIALIPAIVVYSINTSVTISDFFVLFVALLLTP
                     IIPIIIALTIGILIVAVSSKSKHNNILALFLSIIGILLFVYVSSKLQTMDTSQIADIS
                     TMLTNYFNQFYPPAFLFSKALLNNDWISLGIFVLCSFLILLAFVAVVSYFYKQLNTRA
                     FSHHAGKNFHLGELTVSSPFMALYKRELARFFSCTIYALNLFFFFFFFFFFF"
BASE COUNT          570 a          279 c          305 g          633 t
ORIGIN      
        1 gcgtttactt tattctacta ctatcaccga tacctgcaat ttgctattgg tcacatagaa
       61 aaaagaaaac ggggaggaaa taaatatgag taaaaatatt cttgaaatca agcactatac
      121 taaaatatat tccgataaca agaaagctgt tgatgattta aacattacag tgaaaagcgg
      181 agaaatccat gcttttattg ggcataatgg cgcaggaaaa acaacaacca tacgtgctat
      241 cgtcggtgtt atggattttg aacaaggaga aatcctaatt gacagtcatt ctataaaaaa
      301 agaacctgtc ttatgtaagt cgcttaccgc ttatattcca gataatccag atttgtatga
      361 atacatcaca ggtattcaat atttgaatta tatttgtgat atgttcggtg tttcagaaat
      421 tgaacgaact tctggaatac aaaaatatgc ggaacttttt aaaataacag atagtttagg
      481 agatttaatc agctcttatt ctcatggaat gaaacaaaaa ctagctttga tcggggcttt
      541 tattcataaa ccaaagctat ttgtttttga cgaaccattc gttggtctag acccagaagc
      601 gtcatttaat ttgaaaagca taatgaaaga agcctgccaa aatggaagtg ctattttctt
      661 ttctactcac gtattagaag ttgctgaaaa attatgtgat acggtttcaa ttatcaatag
      721 cgggaaactg attgcaagtg gaactatgga ggaaatcaaa ggtcagcaca gcctagaaga
      781 tgtctttatg gaggtcgttg aaaatggcaa agaaatataa actcttatta aaaatgcagt
      841 tatacagctt gtttggctta aaccgcttat tacattctca cgacaaaaaa gagaaaaatc
      901 gcactttagt tattggaata gctggtctgg caattatcgg aattttagtc tatttaagtg
      961 gctatatatg ttctgtgttt gcagacgcag ggttagtaga agcaatccct acgttaataa
     1021 tcgtgattta ttctttgata gttttattac tgactttttt aaaaagttct ggggcattag
     1081 tcggcttaaa ggattatgat atggtaatgt cgttacccgt caataataca tctgttgttt
     1141 tgagcagact taccatgctc tatattatga gcttggtaat tggtatgatt gcattgatac
     1201 cagctattgt tgtttacagc ataaatacat ctgttacaat cagtgatttt tttgtattgt
     1261 ttgtcgcatt attattaaca ccaatcattc ccataataat agctttgaca atcggtattc
     1321 ttattgtggc ggtgtcatca aaatcaaagc ataacaatat attagcttta ttcttaagca
     1381 ttataggcat actgcttttt gtttatgtca gctccaaatt acagacaatg gacacttcgc
     1441 aaatagcaga tattagcact atgcttacaa actattttaa tcagttttat ccaccagcct
     1501 ttttattttc aaaagcatta ctaaataacg actggataag tcttggcata tttgtacttt
     1561 gttcattttt aatattgctt gctttcgttg ctgttgtttc atatttttat aaacagctaa
     1621 atacaagagc tttttcacat catgcaggca aaaatttcca tttaggagaa ttaactgtat
     1681 cttctccgtt catggcacta tacaagagag aactagcccg ttttttctca tgtacgattt
     1741 atgccttaaa tttatttttt tttttttttt tttttttttt ttttttt
//
LOCUS       DAOMTA010000698         1785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3391_length_1785_cov_1.759538, whole genome shotgun sequence.
ACCESSION   DAOMTA010000698 DAOMTA010000000
VERSION     DAOMTA010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_1785_cov_1.759538"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLK68_14630"
     CDS             complement(<1..244)
                     /locus_tag="PLK68_14630"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM91685.1"
                     /translation="MATAFGCQVQYYSTSGKNNQQNYQQVDFDTLLESSDIISIHAPL
                     NSQTENLFDQSAFAKMKDSAYLINVGRGKIVNEADLV"
     gene            complement(292..1074)
                     /locus_tag="PLK68_14635"
                     /pseudo
     CDS             complement(292..1074)
                     /locus_tag="PLK68_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6521 family protein"
     gene            complement(1168..>1785)
                     /locus_tag="PLK68_14640"
     CDS             complement(1168..>1785)
                     /locus_tag="PLK68_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM91686.1"
                     /translation="HDVIVNGFNIFKRQKSKVIAFLLNESIKVFEEEKIVLIKIDSTV
                     EGNLARGKVGMKQLNNIDEELAAIKNLGDYTIVAMLHHHLFPITRDDFLKQKWREKIF
                     VGKIMDSSKALVDSRDLVEWLRKHQIQYVLHGHKHLPFFSSQEGMYVIAAGSSCGSGA
                     KESKSRYLSYNVLKYDHRYKRFKYCFIIYDDMTMQDRQRIVINIF"
BASE COUNT          595 a          358 c          196 g          636 t
ORIGIN      
        1 caactaaatc cgcttcattt acaatttttc ctcttccaac attaattaaa tatgctgaat
       61 ccttcatttt tgcgaaagct gattggtcaa aaagattttc tgtttgacta tttaaaggtg
      121 catgaatact aataatgtca cttgattcca gtaatgtatc aaaatcaacc tgttgataat
      181 tttgctggtt attttttcca cttgtagaat aatattgaac ctgacagcca aatgcggttg
      241 caatttcagc aacttacggc caatatcgcc cattccaacg attccccaag ttttaccagt
      301 caattcgtgg aaagtgtatc ctaaatgggt aaacatagag cttgatgcat aattaccgct
      361 ctcaacataa ttatggtaat aatgattttt attgtataaa tctaataata aagcgaatgt
      421 atgttgtgct actgcatttg ttgaatagcc tttaacattg catacggtta taccggcagc
      481 cttacaatat tcaagatcaa tattattaac acctgtcgca gtaacacaaa taagttttaa
      541 tttatgagca tcttttaact gttcctcttt caatatatgc ttattagtaa ttacaacact
      601 cgcatcttta atccgctcag gaacctcact aacttgactt tgatgatata ttgttacatc
      661 accatatcga tttaatttgc tgacatcaat atcactaccc agtgtctcac tatccaaaaa
      721 gacaatctta aaatcacttt tcatcattgt aactacttca tcaaaacctt ttccttgtat
      781 catttttcct aaaataatag cttcatttaa atcaagaatt tttgatggtc gacaatttcc
      841 gctcattacg ccatcattga tttgaaaatc aagatcttta tttaataaag aaattcctaa
      901 actgcgactc gttacctctt ctaattcttc aacactattt agaatttgat taacaaattc
      961 agaatttttt tgaatacaag ccctaataca actactaaaa tctttgctta tctttacctc
     1021 ttctctaaaa atatcatcaa ataataatgg taaaacaaga ttataaagat ccatcggtac
     1081 agctccataa cctttagtaa agcaatacaa taaagcactc cctaattttt catctttcat
     1141 tccatctact aacttcattt ttctctccta aaaaatattt ataactattc tttgacggtc
     1201 ttgcatcgtc atatcatcat aaatgataaa acagtattta aatcgtttat aacgatggtc
     1261 atattttaat acattataac ttaaatatcg actctttgat tcttttgctc cactgccaca
     1321 tgaagaacca gcagcaatca catacatccc ttcttgactg ctaaaaaatg gtaaatgttt
     1381 atgaccatgc aaaacatact gaatttggtg ttttcttaac cattctacta aatcacggct
     1441 gtctactaat gccttagaac tatccataat tttcccaaca aaaatctttt cacgccattt
     1501 ttgctttaaa aaatcatcac gggtaattgg aaacagatga tgatgcagca ttgcaacaat
     1561 tgtataatct cctaaatttt taatagcagc aagctcttca tcaatattgt ttaattgttt
     1621 cattccaact ttaccacgcg ctaaattacc ttctacagtt gaatcaatct taattaaaac
     1681 aatcttttct tcttcaaata ctttaatgct ttcatttaac aaaaaggcga ttactttact
     1741 tttctgacgc ttaaaaatat taaaaccatt aacaattaca tcatg
//
LOCUS       DAOMTA010000699         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3400_length_1782_cov_2.037058, whole genome shotgun sequence.
ACCESSION   DAOMTA010000699 DAOMTA010000000
VERSION     DAOMTA010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_1782_cov_2.037058"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLK68_14645"
     CDS             <1..380
                     /locus_tag="PLK68_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91687.1"
                     /translation="ILTSCIQQKRHYFLLSRLILKNLILGNKNIPKKYRKTFDNKHYN
                     TIIELLVTQYGIMEKLTSATKKKAALYKLKHQQTIDYLNSKEVLTEIQEEKVKLFLEN
                     TKVLKKNADKNNIRDKVVLKRKG"
     gene            505..756
                     /locus_tag="PLK68_14650"
     CDS             505..756
                     /locus_tag="PLK68_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91688.1"
                     /translation="MNNLIALPIASMNNGDNLKIKFQLSQLIKIYGQTCVDNAIHGQT
                     YDIVRILECQRHVENLMLTFGTVLVMKQFGLLTQKVKAG"
     gene            859..1035
                     /locus_tag="PLK68_14655"
     CDS             859..1035
                     /locus_tag="PLK68_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91689.1"
                     /translation="MTFEQFIEYRNKIVIRLRARKKHFKSNKPNYYEIRLEQIDSQYP
                     EFKKLGVINLKVKE"
     gene            1041..1652
                     /locus_tag="PLK68_14660"
     CDS             1041..1652
                     /locus_tag="PLK68_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91690.1"
                     /translation="MSYNLNYFKNYGSEDYPYWQPSDKDTADIATHSSLVYGDYCNTG
                     AVGKSNVEHIMSLTDLVERTGLYELNDAYNTTGVAIPIKALDDPELVEILEALDNYAI
                     LDDTAYYETEQEMKDEVWESYARRDFEKFLIDKGLLSENKDFEDEYIDSIFGELSQKT
                     NYDIAQPDGDSYYYNFDSMGGLYDKAIDILKNIINNAPTHTLS"
BASE COUNT          694 a          254 c          311 g          523 t
ORIGIN      
        1 ctattttaac ctcatgcatc caacaaaaaa ggcactattt tttattgtca agacttattt
       61 taaaaaatct cattttagga aataaaaaca tacctaaaaa ataccgcaag actttcgaca
      121 ataaacatta taatactatt attgaattat tagtgactca atatggtatt atggaaaaat
      181 taactagcgc aacaaagaaa aaagcggcat tgtataaatt aaagcatcaa caaactattg
      241 actatctaaa tagtaaagaa gtactaacag agatacagga agaaaaagta aaattatttt
      301 tagaaaatac taaagtatta aaaaagaatg ccgataagaa taatatcaga gacaaagttg
      361 tcctgaaaag aaaaggatag aaacatgaaa acacttatga ttgtcttagc tttagttgta
      421 gttttactag gcttcgagac tttgagagtc ttatcaattg acactagatc aaacattgaa
      481 acgatagtga gaggaaacta atatatgaat aatttaatcg ctttacctat tgcgagcatg
      541 aataatggcg ataatctaaa aataaaattt caactatcac aattaattaa aatctatgga
      601 cagacttgtg tcgacaatgc tattcatggt caaacttatg acattgtaag aattttagaa
      661 tgtcaaagac acgtcgaaaa cttaatgttg acctttggta ctgttttggt tatgaaacaa
      721 ttcggattac taacacaaaa agtaaaggca ggataaaatg aaatatgaat tttatcacaa
      781 tgagactggc gaaattagat caatgtcact aaaggaacta atagaacttg acgactattt
      841 atcatgggtg gttttaaagt gacctttgaa caatttattg aatatcgaaa taaaattgtt
      901 attaggctta gggctagaaa gaaacatttt aagtctaata aaccgaatta ttacgaaatt
      961 agactagagc agattgatag tcaatatcct gaatttaaaa aattaggtgt tatcaattta
     1021 aaagtaaagg aataataaaa atgtcttaca atttaaatta ttttaaaaat tatggctctg
     1081 aggattatcc atattggcaa cctagcgaca aggataccgc agatatagca actcactctt
     1141 cgctagtcta tggtgactat tgcaatactg gggcggtagg gaagtctaac gtagaacata
     1201 taatgagtct aaccgatcta gtcgagcgta ctggactata tgaactaaac gacgcgtata
     1261 acactactgg cgtggcaatc cctattaagg ccttggatga ccctgaacta gtcgagatac
     1321 tagaggcctt ggacaattat gctatcctag acgatacggc ctattatgag actgagcaag
     1381 aaatgaaaga cgaggtgtgg gaaagttacg ctagacgtga cttcgagaaa ttcctaattg
     1441 ataaaggact attgtctgaa aataaagact ttgaggacga gtatatcgac tcaatatttg
     1501 gagaattatc tcaaaaaact aactacgata tagcacagcc agacggtgac tcatattact
     1561 acaattttga ttcaatgggt ggtttatatg ataaggctat tgatatttta aaaaatatta
     1621 ttaacaatgc acccacgcac accctttcct aaaaattgaa ggataaaaag atggtctcaa
     1681 aaaatgaatt tttagaattt tttaatagtg atgattttta cgaaaatttt aatgatagcg
     1741 aattgaaaga tatattccta acagttctaa ggggcagtct tg
//
LOCUS       DAOMTA010000700         1778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3410_length_1778_cov_1.760882, whole genome shotgun sequence.
ACCESSION   DAOMTA010000700 DAOMTA010000000
VERSION     DAOMTA010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_1778_cov_1.760882"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1778
                     /locus_tag="PLK68_14665"
     CDS             192..>1778
                     /locus_tag="PLK68_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HRM91691.1"
                     /translation="MVVVVLKAATSFAGIDYNERKNEEGKSELLVAENFAMNPDNLKK
                     SDYIAYMESVCRTNPAVKAKQFHAVISCKGREYSAEDLKNVALQYINKMGYGNNPYMI
                     YFHSDTENNHVHIVSTRVQKNGQKVKDNMEAVRSQKVINQIMNVDLALKAKDDISKYM
                     EFSFSTVQQYKLLLEQSGWKLREKDGLLILYKGGEKHASIQLEQIKDKAKQYTPDEER
                     RKQITALLFKYKQGLSYTELQTLMKDKFGINLVFHTGKGHTKPYGYTLIDYRNKRVLK
                     GGEVMDLKELLVEPNKQAKVEHCNSIINLLLKDGTKYTMDSFKKLMLDYGYRFSMNGT
                     IFINGDDKALLVLDKKLLKELRYNSRVYEANKFNVATLKEAELLSRIYHVKKDDILIQ
                     EHMDKKNTVYSDMINSYLAHSSDLHSTLREKGILFVEDDNLVYLIDKKNKVIVSTDDL
                     GINIIKDGYSKDRITLVTLDKMERINVEQDSELARGFNLIDAICDILSQHINVQQDKS
                     PNRKKKRGQQMCTAATYKTAD"
BASE COUNT          693 a          184 c          342 g          559 t
ORIGIN      
        1 tttccgggtt ctgcccggca tggacgataa gagaacatta ttatgatacc aaaaaacttt
       61 agggaaaaat attatatata taaataatat atgaatatat aatatttgta tatttattat
      121 atgaataaat tatattgaaa tattctttta ttgaatattt gtttgtatat ttgcggtgtg
      181 aagttataaa tatggtagta gtagttttaa aagcggcaac ttctttcgct ggtattgatt
      241 ataatgagcg aaagaatgag gaaggcaaga gtgaacttct tgttgcagaa aattttgcta
      301 tgaatccaga taatttgaag aagtcggatt atatagctta tatggaatct gtctgtagga
      361 ctaatccagc ggtcaaggca aaacaatttc atgcagtgat ttcatgtaag gggcgtgaat
      421 attcggcaga ggatttgaaa aatgtagctt tgcaatatat aaataaaatg ggttatggaa
      481 ataatcctta tatgatatat tttcattctg atacagaaaa taatcatgta catattgttt
      541 ctacacgagt acagaagaac ggacagaagg taaaagataa tatggaagct gtgcgctcac
      601 agaaagtcat aaatcaaata atgaatgttg atttagcttt gaaggctaaa gatgacattt
      661 caaaatacat ggagttttct ttttctacag tgcagcaata taagttgtta cttgaacaat
      721 caggatggaa attaagagaa aaggatggat tattaatttt gtataagggt ggggaaaagc
      781 acgcttcaat tcaattagag cagataaaag ataaagctaa acaatataca cctgatgaag
      841 aaagaagaaa acagataaca gctcttttat ttaaatataa gcaaggactt tcctatacag
      901 aacttcaaac tttgatgaaa gataagtttg gtataaattt agtctttcat acaggaaaag
      961 gacatactaa accttatgga tatacattaa ttgattatag aaataagaga gttttgaaag
     1021 gtggggaagt tatggatttg aaggaacttt tagtagagcc taataaacaa gcaaaagttg
     1081 aacactgtaa tagtataatt aatcttcttt tgaaagatgg aacaaaatat acaatggata
     1141 gtttcaagaa attaatgtta gattacggtt ataggttttc aatgaatggc acaatattta
     1201 taaatggtga tgataaggct ttattggttc ttgataaaaa actgttgaaa gaacttagat
     1261 ataatagcag agtgtatgaa gcaaataaat ttaatgttgc tacacttaaa gaagccgagt
     1321 tgttaagtag aatttatcat gtgaaaaaag atgatatttt gattcaagag catatggata
     1381 aaaaaaatac tgtctattct gatatgataa atagttattt ggctcatagt tcagatttac
     1441 attctacatt aagagaaaaa ggaattcttt ttgttgaaga tgataattta gtttatctaa
     1501 tagataaaaa gaataaagta atcgtaagta ctgatgattt aggaatcaat attataaaag
     1561 atggctatag taaagatagg attactctag ttactcttga taagatggaa agaataaatg
     1621 ttgaacaaga ttcagaactg gcaaggggat ttaatctgat tgatgctatt tgtgatattc
     1681 ttagccagca tataaatgta cagcaagata aatctcctaa taggaaaaag aaaagaggac
     1741 agcaaatgtg cacagcagca acttataaga cagcagat
//
LOCUS       DAOMTA010000701         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3417_length_1775_cov_1.961047, whole genome shotgun sequence.
ACCESSION   DAOMTA010000701 DAOMTA010000000
VERSION     DAOMTA010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_1775_cov_1.961047"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLK68_14670"
     CDS             <1..752
                     /locus_tag="PLK68_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM91692.1"
                     /translation="YNTCFALKNKNEYLLVDAGGGNGILKQLELAKIELSQITNMIVT
                     HSHTDHVLGVVWIFRMVATKIKSGEYDGNFNIYCHDELVSTIKTIIKLTVQERLYNLI
                     DERIFINEVQDGQKITICEHLVTFFDIYSTKAKQFGFSIELDDGKLTCLGDEPFNELC
                     YQYAVDSKWLLSEAFCLYQERDFFKPYEKHHSTVREASELAQTLNIKNLILYHTEEKN
                     LSHRKKLYTDEAKQYFNGNIFVPDDLEEYRL"
     gene            763..999
                     /locus_tag="PLK68_14675"
     CDS             763..999
                     /locus_tag="PLK68_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRM91693.1"
                     /translation="MTLDKLIPGMSGKVTIVHGEGLLRRRLLEMGLTPKTVVKVRKIA
                     PMGDPIELYLRSYVLTIRKDDAAMIEVEVISDAN"
     gene            989..>1775
                     /locus_tag="PLK68_14680"
     CDS             989..>1775
                     /locus_tag="PLK68_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRM91694.1"
                     /translation="MPTKTIALIGNQNCGKTTLFNALTGSNQHVGNFPGVTVEQKSGT
                     IKKHPSIKLVDLPGIYSLTPYTMEEIVSTDFLIKEKPSMIINIIDATSIERNLYLTMQ
                     LLELNIPMILALNMMDEVISSGNSIDVEGLQQALGIRVIPLSASKNEGIEELIEAIET
                     TLKENNCSHLDLCSGEIHKAIHSITHLIEDNAVKAKLPKRFAVTKIIEGDKDIIRQLH
                     LDVQQLHIIKHIIEDMEEKEGLDKDAALVDMRYQVIENITRQTV"
BASE COUNT          646 a          228 c          348 g          553 t
ORIGIN      
        1 gttataatac ttgctttgct ttgaaaaata aaaatgaata tttattggtt gatgccggcg
       61 gtggaaatgg tattttaaag cagcttgaac tggcaaagat cgaattatcc caaataacaa
      121 atatgattgt tacgcatagc cacactgatc atgtattggg agtagtatgg atttttagaa
      181 tggttgcaac caaaatcaaa agtggtgaat atgacggtaa cttcaacata tactgtcatg
      241 atgaattggt ttctacaata aaaacaatta tcaaactaac agtacaagag aggctctata
      301 atttaattga tgagcgtata tttattaatg aagtacaaga tggacaaaaa atcacaattt
      361 gtgaacatct tgtgactttc tttgatattt actcaacaaa agcaaaacaa tttggttttt
      421 cgattgaatt agatgatgga aaattgacat gtttaggtga tgagccattc aatgaattat
      481 gttatcaata tgctgttgat tcaaaatggt tattaagtga agctttttgt ttatatcaag
      541 aacgtgattt ttttaagcca tacgaaaagc atcattcaac agtaagagaa gctagcgaac
      601 tagcacaaac attaaatatc aagaatttga ttttatatca tactgaggaa aaaaatctta
      661 gtcatcgtaa aaaattgtac actgatgaag caaagcaata ttttaatgga aatatctttg
      721 tgcctgatga tctggaagaa tataggttgt aggaggagta acatgacact agataaatta
      781 attccaggaa tgagtgggaa agttacgatt gttcacggag agggcttatt gcgtcgtcgt
      841 ttgttggaaa tgggattgac tccaaaaaca gttgtaaaag ttcgcaagat tgcaccgatg
      901 ggtgatccta ttgaacttta tttacgaagt tatgttttaa cgattcgtaa agatgatgca
      961 gcgatgattg aagtagaggt gattagtgat gccaactaaa acaatagctt taattggaaa
     1021 tcagaattgt ggtaaaacaa ctctttttaa tgctttaact ggttcgaatc agcatgttgg
     1081 taattttcca ggtgtaacag ttgaacaaaa atctggaact attaaaaaac atccatcgat
     1141 taaattagtt gatttgccag gaatatattc attaacacca tatacaatgg aagaaatcgt
     1201 ctcaactgat tttttgatta aagaaaaacc atcaatgata ataaatatta tcgatgcgac
     1261 tagtattgaa cggaatttgt atttaacaat gcagctttta gaattaaata ttccaatgat
     1321 tttggcgctg aatatgatgg atgaggttat tagtagtggt aatagcattg atgtagaagg
     1381 attacaacag gcgttaggaa tacgggttat tccgttatca gcaagtaaaa atgagggaat
     1441 tgaagaattg atcgaggcta ttgaaacaac tctaaaagaa aataactgtt cacatttaga
     1501 tctttgttca ggtgagattc acaaagcgat acattcaata acccatttga ttgaagataa
     1561 tgcagttaag gcaaaactac caaaacgttt tgccgttaca aaaattattg agggtgataa
     1621 agatattatc aggcaattac atcttgatgt tcagcaactt catattatta aacatattat
     1681 tgaggatatg gaagaaaagg aaggcttgga taaagatgcg gccttagtgg atatgcgcta
     1741 tcaagtaatt gaaaatatca cacgacaaac tgttt
//
LOCUS       DAOMTA010000702         1773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3422_length_1773_cov_3.516298, whole genome shotgun sequence.
ACCESSION   DAOMTA010000702 DAOMTA010000000
VERSION     DAOMTA010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3422_length_1773_cov_3.516298"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLK68_14685"
     CDS             complement(<1..580)
                     /locus_tag="PLK68_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91695.1"
                     /translation="MSKIITAITGWFRSLLWSIAEIFLFLLDCLWQVVLKVGTLNIGG
                     SKVTTRFFAVMSVCMLVFIVFKLVKIYIKFFIDNDYRERINLSKVLISLSLAATLISG
                     LPLGYKIVCNMSSEAIANVNYWIPSDFAEASKDIDKINMSDVLIQSGRVNLSNREADL
                     GEEIKLDENFDINATYDRSDYVNDLFSNIGSLD"
     gene            complement(617..922)
                     /locus_tag="PLK68_14690"
     CDS             complement(617..922)
                     /locus_tag="PLK68_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91696.1"
                     /translation="MDKLLVLKNKAMMAPVVLSGINEGELEKWIKGYTDPITNLLIWL
                     IPIGGVIASLVTIVVWFTKDEEMKQRDNPYRIVKKIVFWCLVGEGMLAIFKALGITS"
     gene            complement(926..1177)
                     /locus_tag="PLK68_14695"
     CDS             complement(926..1177)
                     /locus_tag="PLK68_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91697.1"
                     /translation="MQKRYIVGVELAVLLVLFLLMSFFWSIQQALLVIVPLGICTFEG
                     TILIWLSCIITRHFKQKFNEQKETFIEEFDKTKDYYRGR"
     gene            complement(1150..1719)
                     /locus_tag="PLK68_14700"
     CDS             complement(1150..1719)
                     /locus_tag="PLK68_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91698.1"
                     /translation="MKTLERLLNDRVTIQLKSEVERYLEGALEGGEYLIHTDTECFNN
                     ITVKVIGSDEKTLAYMSGYYIQFHDYKDLIFNADGHDENFYNIVSNVEEHVSKIKEPQ
                     SIYFNLFINAKMSCAVEAQLYIHLKDMIYTVLHYVPEYVAGYIRKETWNMVGRELTKQ
                     GFKVINEDIDCLEILCKGNEECRSVTLWG"
BASE COUNT          598 a          358 c          208 g          609 t
ORIGIN      
        1 catctaatga accaatatta ctgaataagt catttacata atctgacctg tcataggttg
       61 cattgatatc aaagttttca tcgagcttta tctcttcacc cagatcagct tcacgattag
      121 aaagattgac tcgacccgat tgaatcagta catctgacat attgatttta tctatatctt
      181 tagaagcttc tgcaaaatca gaaggaatcc agtaattgac attagcgatt gcttcactcg
      241 acatattgca aacaatctta taaccaagtg gtaatcctga tattaaagtt gctgctaaag
      301 aaagactgat cagcacttta gataaattga ttctttctct atagtcatta tcaatgaaaa
      361 acttgatata aatctttact agtttaaaaa caataaatac cagcatacat acactcataa
      421 ccgcaaagaa cctagtcgtt actttactgc caccaatatt taatgttccc acctttaaaa
      481 caacctgcca taagcagtcc aataaaaaaa ggaatatctc tgcgatactc cataataacg
      541 atctaaacca tcctgtaata gctgtaataa tctttgacaa tgtatcacca cctctcactt
      601 attacagtca gttattttat gatgtgattc ctaatgcttt gaagatggca agcatacctt
      661 ctcctacaag acaccagaaa acaattttct taacaattct gtatggatta tctctttgtt
      721 tcatttcttc gtcttttgta aaccacacaa caattgtaac aagactggca ataactccac
      781 caattggaat taaccaaatt aataagtttg taattggatc agtataacct ttgatccatt
      841 tttctaattc accttcatta atacctgata gcactacagg tgccatcata gctttattct
      901 ttaacactaa tagtttatcc attgtttacc tccctcgata atagtctttt gttttatcga
      961 attcttctat aaatgtttct ttttgttcgt taaacttctg cttaaagtgt ctggtgatga
     1021 tacaactcaa ccatataaga atcgttcctt caaatgtaca gatacctaaa ggaacaatca
     1081 ccaataatgc ttgttgtatt gaccagaaga atgacatcaa aagaaaaaga accagcaata
     1141 ctgccagttc tacccccaca atgtaacgct tctgcattct tcattaccct tacataaaat
     1201 ttctaaacaa tcaatatctt cattaatgac tttaaatccc tgtttcgtaa gttctcttcc
     1261 taccatattc catgtttcct tacgaatata tccggcgaca tattcaggaa cataatgaag
     1321 aactgtataa atcatatcct tcagatgaat atataattgt gcttctactg cacaagacat
     1381 ctttgcatta ataaataaat taaaatagat gctttgtggt tccttgatct ttgatacatg
     1441 ttcttcaaca ttacttacta tgttataaaa gttttcatca tgaccatcag cattaaaaat
     1501 cagatcctta taatcatgga attgaatata ataaccagac atgtaagcta atgttttttc
     1561 atctgatcca ataaccttaa cagtaatatt attgaaacat tcagtatcag tatgaattag
     1621 atattcacca ccttctaatg ctccttctaa atatctttca acttctgatt tcaattgaat
     1681 tgttactcta tcatttaata atctttctaa tgttttcatt ttacttctcc atataaaaca
     1741 gaaaaggatt cggttgaccc gaatcctaat aat
//
LOCUS       DAOMTA010000703         1773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3424_length_1773_cov_1.902794, whole genome shotgun sequence.
ACCESSION   DAOMTA010000703 DAOMTA010000000
VERSION     DAOMTA010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_1773_cov_1.902794"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLK68_14705"
     CDS             complement(<1..312)
                     /locus_tag="PLK68_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91699.1"
                     /translation="MIGKLTEGNITRALIKFSIPMILGNLLQQLYNVADTFIVGHYIG
                     TDALAAVGSSFTIMTFLTSIILGLCMGSGILFSMFYGAKQLDKMKTSFFVSFVGIGIF
                     SI"
     gene            464..>1773
                     /locus_tag="PLK68_14710"
     CDS             464..>1773
                     /locus_tag="PLK68_14710"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM91700.1"
                     /translation="MTFNKDFLWGGAIAAHQAEGAWQEGGKGISCSDVETAGNNVTGA
                     PRRLTDGVIEGEDYPNHVGVDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPRGDEET
                     PNEEGLQFYDDLFDECHKYGIEPIITLSHFEMPWTLAKEYGGFRNREAIDMFVKFAKV
                     CFERYQHKVKYWMTFNEINNQADVSQHNLIQEGAVLLKKDDDAEYLMYLSAHHELVAS
                     ALAVKAAHEINPDLQVGCMIGMNAVYPASPNPTDVMNALGAMHQKYWYVEVHARGHYP
                     NHILKKFERKGYDFITEEDKKILSQGKVDYIGFSYYMSFASKYQGRNEKTYDYFSEDF
                     VRNTHLKASDWGWQIDPLGLRWCLNWFHDRFELPMMIVENGFGAYDKVEADGTIDDQY
                     RIDYLAAHIEAMRDAVDYDGVELLGYTMWSPIDIVSASTGEYDKR"
BASE COUNT          608 a          279 c          342 g          544 t
ORIGIN      
        1 aattgaaaaa atgccgattc ctacaaaaga gacaaaaaaa gatgttttca ttttatctaa
       61 ttgtttagcc ccatagaaca tcgaaaataa aattccactc cccatgcaaa gacctaagat
      121 aatggaagtt aaaaaagtca taatcgtaaa agatgagcca actgctgcca gagcatcagt
      181 tccgatataa tgtccaacaa taaaagtatc agctacatta taaagctgct gtaaaagatt
      241 tcctaagatc attggaattg aaaatttaat taatgccctg gtaatatttc cttctgttaa
      301 tttccctatc ataaaatcac cccattactt acataataaa tatatcatcc aataaagtca
      361 attaaagaaa attatgtaag atatttatca ttcttatcat tatttccgtt atctttattg
      421 atttcgtagt ataatatatt tataaataag gaggtcgata tagatgacat ttaataaaga
      481 ttttttatgg ggtggtgcaa ttgcagcaca tcaagctgaa ggagcttggc aagaaggtgg
      541 aaaaggaatt agttgctctg atgtcgaaac agcagggaat aatgtaactg gtgcgccaag
      601 gcgcttaact gatggcgtca tcgaaggaga agattatcca aatcatgttg gggtggattt
      661 ctaccatcgc tataaggaag atattgcttt attcgctgaa atgggattca aagcatttag
      721 aacttctatt gcctggacac gaatttttcc tcggggtgat gaagaaacac ctaacgaaga
      781 gggtttacaa ttttatgatg atttatttga tgaatgtcat aaatatggaa ttgagccaat
      841 tataacatta tcgcacttcg aaatgccttg gacgcttgca aaagagtatg gtggttttag
      901 aaatcgtgaa gcaatcgaca tgttcgttaa atttgccaaa gtatgctttg aaagatacca
      961 gcataaagtt aaatactgga tgacttttaa tgaaatcaat aatcaagctg atgtttctca
     1021 gcacaactta atccaagaag gagctgtact tttaaaaaaa gatgatgatg cagaatattt
     1081 aatgtattta tctgctcacc atgaattagt cgcttctgct ttagctgtta aagccgctca
     1141 tgagatcaat cctgatttac aagttggatg tatgattggg atgaatgctg tttaccctgc
     1201 atcacctaat ccaactgatg taatgaacgc tttaggagca atgcatcaaa aatactggta
     1261 tgttgaagtc catgcccgtg gtcactaccc aaatcatata ttgaaaaaat tcgaaagaaa
     1321 aggttatgat tttattactg aagaagataa aaagatttta tctcaaggta aagtggacta
     1381 tattggattc tcatattaca tgagttttgc ttcaaaatat caaggacgta atgaaaaaac
     1441 atatgattat ttcagtgagg actttgttcg aaatactcat ctaaaagctt cagactgggg
     1501 ttggcaaatc gatccactag gattacgctg gtgcctaaac tggttccatg accgttttga
     1561 gttaccaatg atgattgttg aaaatggttt tggtgcttat gacaaagtag aagcagacgg
     1621 aacaatcgat gatcagtatc gtatcgacta tttagcagca catatcgaag ctatgaggga
     1681 tgcagttgac tatgatggag tagaattatt aggttataca atgtggtcac cgatcgacat
     1741 tgtctctgct tctactggtg aatatgataa acg
//
LOCUS       DAOMTA010000704         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3433_length_1769_cov_2.590432, whole genome shotgun sequence.
ACCESSION   DAOMTA010000704 DAOMTA010000000
VERSION     DAOMTA010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3433_length_1769_cov_2.590432"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1769
                     /gene="alaS"
                     /locus_tag="PLK68_14715"
     CDS             <1..>1769
                     /gene="alaS"
                     /locus_tag="PLK68_14715"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRM91701.1"
                     /translation="PCGPDSEIFYDRGEKYDPDGLGTKLFYEELENDRYIEIWNLVFS
                     QYNSQEGIAREDYKELPQKNIDTGMGLERITSIIQGGETNFDTDFFLPIIHEVEKLAN
                     VSYQENKMAYRVIADHIRTVTFALADGALFDNAGRGYVLRRILRRAVRYGKQIGIEKA
                     FMYDLVDVVSEIMKEFYDYLPEKVSYISDLVKKEEEAFHKTLTNGEKLLSSIIAKNTD
                     GVISGKDAFKLYDTYGFPFELTLEIAEESDLKVDEEAFRAELKVQQERSRGARTDSES
                     MASQKPDLMAFDLPSNFEYDPTNINSVVIGLFKDGVKTDRIDDYGEVIFDTTTFYAEM
                     GGQCADTGYVYNENCQGEVINVLKAPNQQHLHFVKLKTGTIRVGDILTLDVDKAKRNK
                     IIANHSATHILQAALKEIIGKHINQAGSYVDDHRLRFDFTHFEKITNEQLKLIENQVN
                     NVIFDGVAVDIAHMTKDEAINSGAMALFDEKYGDKVRVVSIGDYSMELCGGCHVSNSA
                     NIGLFKIEAEESVGSGVRRIEAVTGKTAYQALVQEKETVDIISNILKLKNRKEVVAKV
                     TALTEELSNTKKEIEILSGQLNA"
BASE COUNT          632 a          194 c          377 g          566 t
ORIGIN      
        1 gaccatgtgg acccgattca gaaatttttt atgatcgtgg ggaaaaatat gatcctgatg
       61 gtttaggaac aaagttgttt tatgaagaat tagaaaatga tcgatatatt gaaatttgga
      121 atttagtatt ttcacaatat aactcacaag aaggaatagc tcgtgaagat tataaagagt
      181 tgccacaaaa aaatatcgat actgggatgg gattagaaag aattactagt attatccaag
      241 gcggagaaac aaattttgat actgattttt tcttaccaat tatccatgaa gtagaaaaat
      301 tagccaatgt ttcatatcaa gaaaataaga tggcttatcg agttattgct gatcatatcc
      361 ggactgtaac atttgcactt gctgatggag cattatttga taatgcaggt cgtggatatg
      421 ttttgaggag aattttaaga agagctgttc gttatggtaa gcaaattgga atcgaaaaag
      481 cgtttatgta tgatttagtt gatgttgtaa gcgaaattat gaaagaattc tatgattatc
      541 taccagaaaa agtaagttat attagtgact tagtaaaaaa agaggaagaa gcttttcata
      601 agacgttaac aaatggtgaa aaattattaa gtagtattat tgcaaaaaat actgatggtg
      661 tgattagtgg aaaggatgcc ttcaaattat acgatactta tggttttcct tttgaattga
      721 ctttggaaat tgctgaagaa tcagatttaa aagttgatga agaggctttt agagcagagc
      781 ttaaagtaca acaagaaaga tcgcgtggag cacgtactga tagtgaaagt atggcaagtc
      841 agaaacctga tttaatggcc tttgatttac catcgaattt tgaatatgat ccaacaaata
      901 tcaatagtgt agttattgga ttatttaaag atggtgttaa aacagatcga attgatgatt
      961 atggagaagt catttttgat acaacaactt tttatgctga gatgggtggt caatgtgcag
     1021 atactgggta tgtttataat gaaaattgtc agggtgaagt aattaatgtg ctaaaagcac
     1081 caaatcaaca gcatttacat ttcgttaaat tgaagacagg aacgattcga gttggtgata
     1141 ttttaacttt agatgttgat aaagctaagc gaaataagat tattgctaac catagtgcta
     1201 ctcatattct tcaagcagct ttaaaagaaa ttattggaaa acatattaat caagctggtt
     1261 cttatgttga tgatcatcgt ttaagatttg attttactca ttttgaaaaa ataacaaatg
     1321 aacaacttaa attaattgag aatcaggtta ataatgtcat ttttgatgga gttgctgtgg
     1381 atattgcaca catgactaaa gatgaagcca ttaatagtgg tgctatggcg ttgtttgatg
     1441 agaagtatgg cgataaggtt cgggttgttt caatcggtga ttattcaatg gaattatgtg
     1501 gtggatgtca tgtttcaaac agtgcaaata ttggattatt taaaattgag gctgaagaga
     1561 gtgttggtag tggagtgcgt cgaattgagg cggttactgg aaagactgct tatcaagcgt
     1621 tagttcaaga aaaagaaacg gttgatatta ttagtaatat tttaaaactt aaaaatcgta
     1681 aagaagtagt tgcaaaagtt actgcattaa ctgaagaact ttccaacact aaaaaagaga
     1741 ttgaaatatt aagcggacaa ttaaatgcg
//
LOCUS       DAOMTA010000705         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3435_length_1768_cov_2.416229, whole genome shotgun sequence.
ACCESSION   DAOMTA010000705 DAOMTA010000000
VERSION     DAOMTA010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3435_length_1768_cov_2.416229"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="rsmG"
                     /locus_tag="PLK68_14720"
     CDS             <1..653
                     /gene="rsmG"
                     /locus_tag="PLK68_14720"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRM91702.1"
                     /translation="TPQQVNQFKQYFKILVEWNEKMNLTAITDEEGVYLKHFYDSLTI
                     AFDFDFTDQNIVDVGAGAGFPSVPLKIVYPELKITIVDSLTKRITFLNHLFKELNLSN
                     CQAISARAEDYAKDHRQKCDIVMARAVARLNILDELCLPLVKVGGYFLALKGLKATEE
                     LEEAGKGIVLLGGQVEKSIDFTLTNDNHRSNIIIKKVRDTPAKYPRMFGKIKKQPL"
     gene            662..1429
                     /gene="noc"
                     /locus_tag="PLK68_14725"
     CDS             662..1429
                     /gene="noc"
                     /locus_tag="PLK68_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HRM91703.1"
                     /translation="MLEKVYKQIDIEKISANENQPRTVFDDEKIEELAASIKENGLIQ
                     PIIVRKYNRGYQIVAGERRYRASKLAGLKTVPCVIKDIDDKQVDTLAIIENIQRENLS
                     PIEEANAYKTLIDTYDMNQTELANKVGKKQSTIANKLRLLKLSDDVKHALKSKQITER
                     HARAMIGLEADKQQTVLQEVLKKSLNVKQTESLISKPVKTKPKNKKGPTKVSRNFKIA
                     INTINQATELIQKSGIEVISETEEADDEYIITLRVKK"
     gene            1429..>1768
                     /locus_tag="PLK68_14730"
     CDS             1429..>1768
                     /locus_tag="PLK68_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM91704.1"
                     /translation="MKINDLILAMIDFYQGHPKQIQHLIKVHSFARVIGIDEGLSTQE
                     QERLEVAAIVHDIGIKPAWEKYNSSNGKYQEELGPAEAIKLLNRLNYDEALIERVAYL
                     VGHHHTYSEID"
BASE COUNT          685 a          229 c          337 g          517 t
ORIGIN      
        1 taacccccca acaagtcaat caatttaaac aatattttaa gatattggtt gaatggaatg
       61 aaaaaatgaa tttaacagca attactgatg aagaaggggt ttatttaaaa catttttatg
      121 attcattaac gattgctttt gattttgatt ttactgatca aaatattgtt gatgtgggtg
      181 ctggggcagg atttcctagt gtgccattaa aaattgtata tcctgagtta aaaattacta
      241 tagttgattc actaacaaaa agaatcacat tcttaaatca tttatttaaa gagttaaatt
      301 taagtaattg tcaagctatt agcgcaagag cagaagatta tgctaaagat catcgccaaa
      361 aatgtgatat tgttatggcg cgcgcagtag cccgtttaaa tattcttgat gagctgtgct
      421 tacctttagt gaaagtagga ggatactttt tagcccttaa agggttaaag gctacggaag
      481 aattagagga agctggtaag ggcattgttt tgttaggtgg tcaagtggaa aaatcaattg
      541 attttacttt aacaaatgat aatcatcgaa gtaatatcat tataaaaaag gtaagggata
      601 ctccagccaa atatccacga atgtttggaa aaataaaaaa acaaccccta taggagaagt
      661 tatgttagag aaagtatata aacaaattga tattgaaaaa atttcagcga atgaaaatca
      721 accaagaact gtttttgatg atgaaaagat tgaggaatta gcagcttcaa tcaaagagaa
      781 tggtttgatc cagccgatta ttgtacgtaa atataatcgt ggatatcaaa ttgttgcagg
      841 agaacgtcgt tatcgagcaa gtaagttagc aggattaaaa acagtgcctt gcgttattaa
      901 agatattgat gataaacaag ttgatacatt agcgatcatt gaaaatattc agcgtgaaaa
      961 cttatcaccg atcgaggaag ccaatgcata taaaacattg attgatacat atgatatgaa
     1021 tcaaaccgaa ttagctaata aagtagggaa aaaacagtcg acgattgcta ataaattacg
     1081 gttattaaag ttatcagatg atgttaaaca tgctttaaaa tcaaaacaga ttacagagcg
     1141 ccatgcccgt gcaatgattg gcttggaagc tgataaacag caaacggtat tgcaagaagt
     1201 cttaaaaaaa tcattaaatg taaaacaaac agagtctttg attagtaaac cagttaaaac
     1261 aaaacctaaa aataaaaaag gtccaactaa agtatctcgt aattttaaaa tcgcaatcaa
     1321 tacaatcaat caagctacag aattgattca aaaatcggga attgaagtta ttagtgaaac
     1381 tgaagaagct gacgatgaat atattattac attaagggta aaaaaataat gaagataaat
     1441 gatttaatat tagcgatgat tgatttttat caagggcacc ctaaacaaat tcagcatttg
     1501 attaaagtcc acagctttgc tagagtaatc ggaattgatg aggggttatc tacacaagaa
     1561 caagaaagat tggaagttgc cgcaattgtt catgatattg gaattaaacc tgcttgggaa
     1621 aaatataata gttctaatgg taagtatcaa gaagagttag gaccggcaga agcgattaag
     1681 ctgctaaatc gtttaaatta tgatgaagct ttaatagaac gagttgctta tcttgttggt
     1741 catcatcata cttatagtga aatagatg
//
LOCUS       DAOMTA010000706         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3447_length_1765_cov_1.864327, whole genome shotgun sequence.
ACCESSION   DAOMTA010000706 DAOMTA010000000
VERSION     DAOMTA010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3447_length_1765_cov_1.864327"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1765
                     /locus_tag="PLK68_14735"
     CDS             <1..>1765
                     /locus_tag="PLK68_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRM91705.1"
                     /translation="TKDLKSMPSTNGNTIEEMIQSQAGVSTHNELSTQYNVRGGNFDE
                     NSVYINNIEVYRPFLIHSGQQEGLSVINPDMVEKIGFSTGGFEAKYSDKMSSALDITY
                     KKPTESEAKIQASLMGASCYIGLAAKKISWINGIRYKTSKCLLGSLDTKGEYDPRFFD
                     YQSYLSYQPNKRWTIDFLGNVSTNSYNFTPKDRETDFGTLQNIKKFKVYFDGSEKDLF
                     NTYFGALSVTRNISINTKISFLSSAFYTKEQETYDIQSQYWLTESEATENLGIGTYME
                     HARNYIKTNVESFKIILDRISKAHHIISGLTYKIEHVKENSNEYEMRDSSGYSIPHTG
                     SNLQMTYSLKCLNYLNSSRVEAFIQDTYKFKSLGGTFYSLNYGVRFSRWSYNSESLFS
                     PRLSLTIIPEKYENLTLRFATGLYYQSPFFKEIRDTTTTNGITIATLNSKIKSQRSIH
                     FVIGLDYRFKINDRAYKFTAETYYKALSNLIPYNVDNMRIVYYGNNQSSGHAMGFDLK
                     LYGEFVPETDSWISFSLMNTKEKINGKSIPLPTDQRYAFNFYFTDYFPGTIRWKMSLK
                     MSYADGLPFGAPHKGLEDNNFR"
BASE COUNT          675 a          286 c          302 g          502 t
ORIGIN      
        1 tacaaaagac ctaaaatcta tgccttctac aaatggtaat acaatagaag aaatgataca
       61 aagccaagca ggggtatcta cacataacga attgtcaaca caatataatg taagaggtgg
      121 aaactttgat gagaatagtg tttacataaa taacatagaa gtgtatcgac catttttaat
      181 tcatagtgga caacaggaag gattaagtgt tataaaccct gatatggtcg agaaaattgg
      241 tttttctaca ggaggtttcg aagctaaata ctctgataag atgtcatccg ctctagatat
      301 aacatataaa aagccaactg aatcggaagc aaaaattcaa gccagtctaa tgggtgcaag
      361 ctgttatata ggactagcag caaaaaagat atcatggatt aatggtataa gatataagac
      421 aagcaaatgt ttattaggat cattagatac aaagggtgaa tatgatccac gtttttttga
      481 ctatcaatct tatttaagct atcaacctaa taaacgttgg accattgact ttcttggtaa
      541 cgtatcaacc aacagctata acttcacgcc aaaggataga gaaacagatt ttggcacatt
      601 acagaatata aaaaagttca aggtatactt tgatggaagt gagaaagacc tttttaatac
      661 ttattttgga gccttgagtg ttacacgtaa tataagtata aacactaaaa tatcatttct
      721 gtcgtcagcc ttttatacaa aagagcaaga aacttatgac attcaaagtc aatactggct
      781 tacagagtcc gaagccacag aaaatctcgg tatcgggaca tacatggagc atgccagaaa
      841 ttatatcaag acaaacgttg aaagtttcaa aataatttta gaccgcatat caaaagccca
      901 tcatattatc tcaggattga cttacaagat tgagcatgta aaagaaaata gtaacgaata
      961 tgaaatgaga gattcaagtg gatattcaat acctcataca ggctctaatc tgcaaatgac
     1021 ttattcactg aaatgcctaa attatttgaa ttcttctaga gttgaagcct ttattcaaga
     1081 cacttataaa ttcaaatcac tcggaggaac attttattct ctaaattatg gagttagatt
     1141 cagtagatgg agttataata gtgaaagtct atttagtccg cgactttcac taacaataat
     1201 acctgaaaaa tatgaaaacc taactttaag atttgcaaca ggactttact accagtcacc
     1261 atttttcaaa gaaattagag atactacaac tactaatgga ataacaatag ctaccctaaa
     1321 cagtaagata aagagccaga gatcaataca ttttgtaatc ggtttagatt acagatttaa
     1381 gattaatgac cgagcctata aattcaccgc agaaacttac tataaagcat tgtcaaacct
     1441 aataccatac aatgtagata acatgagaat agtatactat ggcaataacc aaagttctgg
     1501 tcatgctatg gggtttgatt taaaattata cggagagttt gttccagaaa cagattcgtg
     1561 gattagtttt tctctcatga atacaaaaga gaaaataaac ggtaaaagta taccattacc
     1621 tacagatcaa agatatgcat ttaactttta ctttacagac tactttcctg gtacaataag
     1681 atggaaaatg tcattgaaaa tgtcttacgc agacgggctg ccattcggtg cgccacataa
     1741 aggacttgaa gacaacaatt ttagg
//
LOCUS       DAOMTA010000707         1764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3451_length_1764_cov_1.909304, whole genome shotgun sequence.
ACCESSION   DAOMTA010000707 DAOMTA010000000
VERSION     DAOMTA010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3451_length_1764_cov_1.909304"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLK68_14740"
     CDS             <1..530
                     /locus_tag="PLK68_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91706.1"
                     /translation="IKDIKLNSLRQNIGIVQQDVYLFAGTILDNIRYGRFDATDEEVI
                     EAAKKANAHDFIMELPDGYDTDCGQRGVKLSGGQKQRLSIARVFLKNPPILIFDEATS
                     ALDNESEHIVQQSLESLAKNRTTLVIAHRLSTIKNAKRICVLSTKGIEEEGTHDELLA
                     KNGQYATFYKMQFNK"
     gene            1384..>1764
                     /locus_tag="PLK68_14745"
     CDS             1384..>1764
                     /locus_tag="PLK68_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRM91707.1"
                     /translation="MIQILKDKQSMYTINTSLHELPVVISLPHSGTYITSEMAENLMD
                     DVIFPNTDWYLPKLYGFLKELGFTIIINNMNRHLIDVNRDINDKKGSSYKTNLIYTKT
                     TQGALMYNHELSVQEIERRIADYYL"
BASE COUNT          660 a          221 c          311 g          572 t
ORIGIN      
        1 atattaaaga tattaaatta aattcattac gtcaaaatat cgggattgtc caacaggatg
       61 tttatttatt cgctggaaca attttagata atattcgtta tggtcgcttt gacgcaacag
      121 atgaagaagt cattgaggct gctaaaaaag caaatgctca tgattttata atggaattac
      181 cagatggcta tgatactgat tgtggtcagc gtggagtgaa actatctggt gggcaaaaac
      241 agcgattaag tatagctcgg gtttttttaa agaatcctcc aattttaatt tttgatgagg
      301 cgacaagtgc attggataat gaaagtgaac atattgttca gcaatcatta gaatcactgg
      361 ccaaaaatcg aacaacgtta gttattgccc atcgtttatc aacgataaaa aatgctaaac
      421 gcatttgtgt attatctaca aaaggaattg aagaagaagg aacacatgat gaattattgg
      481 caaaaaatgg ccaatatgca accttttata aaatgcagtt taataaatag aagtagaaga
      541 actgaaatga actcagttat ttgggtctcc aaatgaagaa tggtttattt cagaacttct
      601 atttttttat aacaatttaa agatcagtat aaatagttaa aacaagattg tgataatctt
      661 ttttatatca ttattgttgt gttaaaaagc tgagaagagt aaactgtatc tatacgatta
      721 aaggagaaat aggatggaat tatataaaat aattgcccct aataattgga aaagatcaat
      781 gtattgtcag ttttttttga gatagtagtg aacaatgttt ttgtctgaac ttagaagtag
      841 atactgcgaa tttaaaaaag aatttgagag caaaattatt cattcacaat ggttttgacc
      901 tatgcagtaa gtaaatatgc aagtgagata gaggcatgca ggtactgatt ttttgacggt
      961 caaatcgttc tatatgatca aatcgacaca gcccttacat atttagagct agagattgga
     1021 ttatttgaag tagtcatagt tgcaatacag ccaaagatag aagactatat ccagttggct
     1081 acaaaaatgg caaaagaaca aactgaatat ttactgattc actaggaaat aatgtatttt
     1141 aattttcatt acagtctagg ataccatatt aacatatatt acacactaac tttggtaaga
     1201 aggataatga gactctctta ttcgactaga gaaaatattt ctaatatgat ggtgaaatac
     1261 ttttaccatt tttaattcaa acacatcatc tacttgttga tgatctacat atgggtaaat
     1321 gataaaatat agttatcact ttttttaaac taaattaaga tgggaaaata tgagaggaat
     1381 agtatgatac agatattaaa agataaacag tcgatgtata caattaatac atcgttgcat
     1441 gagctgcctg tagtaatcag cttaccgcat agtggaacat atataacttc tgaaatggct
     1501 gaaaatttaa tggacgatgt tatttttcct aatactgatt ggtatttacc aaaactgtat
     1561 ggctttttaa aagaattagg gttcacaatt attattaata atatgaaccg gcatttgatt
     1621 gatgtgaatc gtgatattaa cgataaaaaa ggtagttctt ataagacaaa tctaatttat
     1681 acaaaaacca ctcaaggagc tttaatgtat aatcacgaat tatcagtaca agaaattgaa
     1741 agacggattg ctgattatta ttta
//
LOCUS       DAOMTA010000708         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3461_length_1760_cov_2.181818, whole genome shotgun sequence.
ACCESSION   DAOMTA010000708 DAOMTA010000000
VERSION     DAOMTA010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3461_length_1760_cov_2.181818"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..937)
                     /locus_tag="PLK68_14750"
     CDS             complement(164..937)
                     /locus_tag="PLK68_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008539.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRM91708.1"
                     /translation="MKTGIYYFSATGNSLTTAKLLAASLDGQCDVISLAALHNKQDIE
                     VDYERVGFVFPIYYGDMPYLIRDTIRKMKFKQNTYIFIFTTYRGYPGDVAKRFDNLLQ
                     EKNLSLALSKGIPMPGNSYLSTVEQIKDTLANQKTNIKKLVKSIIEQDKIDYSLLPEV
                     ENSAVYKACNMRGIKVDEKCIGCQTCIKVCPMNNIELIEGKIKIKDNCMTCLACFHWC
                     PTAAIYMSKEKEIERREKYHHPDVRLTDIIKQKYNEFVE"
     gene            complement(1101..>1760)
                     /locus_tag="PLK68_14755"
     CDS             complement(1101..>1760)
                     /locus_tag="PLK68_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM91709.1"
                     /translation="VFQPVILKKSIQGYEIVAGERRCRAAKIAGLVEIPAIIVDFTDQ
                     QMMEIALLENIQRENLNSIEEAKAYQMMMERLNLKQDELAKRIGKSRSYIANTLRLLQ
                     LPEMIQNYVLEGKITMGHARCLITLPQEKAESLAARCIEEGLSVRDVENIVKGIELGN
                     SRKDRPKVEKPKEYVYVEGLLRKKFRTKIKVDEKAVTIKYTDTKDLNRILELMGVIEE
                     S"
BASE COUNT          552 a          340 c          198 g          670 t
ORIGIN      
        1 tagtctatat attcactata ctctaacgac ctagggcact gtcaaccatt catgcaataa
       61 ttaatacttt ttaagaaaat ggcactaaat agctatactc atcaaataaa ttattgtgct
      121 taaatataat aaacagctat tttaaatatc aacaataaga agcttactct acaaattcat
      181 tatacttttg cttgataata tctgttaaac ggacatctgg atgatgatat ttctctcttc
      241 tttcaatttc tttttcttta gacatatata tcgctgctgt tggacaccaa tgaaaacacg
      301 ctaaacaggt catacaatta tctttaattt taatttttcc ttcaatcaac tcaatattat
      361 tcattggaca aacttttata catgtttggc acccgataca cttctcgtca actttaattc
      421 cacgcatatt acaagcttta tatactgcag aattttcaac ttcaggtaat aatgaataat
      481 ctatcttatc ttgttctatt attgatttta caagtttttt gatatttgtc ttttgattag
      541 ctaatgtgtc ttttatttgt tcaacagtac tcaaataact attaccaggc attgggattc
      601 ccttacttag tgctaaactt aaattttttt cttgtaaaag attatcaaat ctttttgcta
      661 catctccggg atatcctcta tatgttgtga atataaaaat ataagtattc tgcttaaatt
      721 tcatttttct aatagtatcc cttataagat atggcatatc cccatagtat attggaaaaa
      781 caaaccccac tcgctcataa tcaacttcga tatcttgttt attgtgaagt gctgcaagcg
      841 atataacatc acattgccca tcaagagatg ctgctaatag ttttgctgtt gtcaaactat
      901 tgccggttgc tgaaaaataa tagattcctg tcttcatatt aaccccctat aaatacttta
      961 ctacttttag aattcttaat tataagtata accaaattaa ctaattttga tagtattctt
     1021 tcaaacacag aaaaagaggg gctcatgttt ttatgaactc ctcacgtgtc atcaaatact
     1081 taacttaatg atatcgaagc ttaactttcc tcaattactc ccattaactc taaaatccga
     1141 tttaaatctt tagtatcagt atatttgatt gttacagcct tttcatcaac cttgatctta
     1201 gttctaaatt tctttcttaa taacccttca acatagacat attcctttgg tttttcaact
     1261 ttaggacgat ctttacgact attgcctaat tcaattccct taacaatgtt ttcaacatca
     1321 cgaactgata agccttcttc aatacaacga gcagctaaac tttcggcttt ttcttgagga
     1381 agagtaatta aacaacgagc atgccccatt gtaatttttc cttcaagtac ataattttga
     1441 atcatttcag gtaattgtaa taatcttaac gtatttgcaa tataacttct tgatttacca
     1501 attcttttag ctaattcatc ttgcttaaga ttcaatctct ccatcatcat ttggtaggct
     1561 ttagcttctt caatagaatt taaattctct ctttgaatat tttccaataa tgcaatttcc
     1621 atcatttgtt gatctgtaaa atctacaata attgcaggta tttctactaa tcccgcaatt
     1681 ttagccgccc gacatcttct ttcaccagcc acaatttcat atccctggat tgatttcttt
     1741 aaaatcaccg gttgaaaaac
//
LOCUS       DAOMTA010000709         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3499_length_1746_cov_1.723241, whole genome shotgun sequence.
ACCESSION   DAOMTA010000709 DAOMTA010000000
VERSION     DAOMTA010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_1746_cov_1.723241"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1043
                     /locus_tag="PLK68_14760"
     CDS             240..1043
                     /locus_tag="PLK68_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM91710.1"
                     /translation="MKNITKMFLFILSFYLVLGSLWIGKDVIIHYQNIDGLPILGYHG
                     VLEDKDKEKYFANYPYCMSLSEFEAQMKYLNQNNYHTLTMDEINDYYQNHTPLPKKSV
                     ALTFDDGLLNFKTVVKPILEKYNFKATCFVIGYKTTVKNSQNPYKHQYLRKSDLVNDE
                     YVEYYSHSYNLHHNTKYPNTKLIETLSTQEIINDFKQNENIVSSKYFAFPYGRTCDNA
                     NEALIKANVSLAFGYNQNRTMTYHDDKYLLPRYLMFSKMPMFYFKWIVE"
     gene            complement(1085..>1746)
                     /locus_tag="PLK68_14765"
     CDS             complement(1085..>1746)
                     /locus_tag="PLK68_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91711.1"
                     /translation="MRANQEYYLYADVQANGNYPNWYLKNLEKRDIHLNFSKEELDII
                     KNNTVDYISFSYYLSLVASHDPARKVGEGNLKGGVENPYLEKSDWGWAIDPIGLRITM
                     NEIYYRYNLPILISENGFGANDILTVDNQIHDEYRIKYLQKHINELKKAIELDGVICL
                     GYLSWSPIDMISAGTSEMNKRYGYIYVDYDDYGNGSGKRYLKDSFYWYQKVIASNGKV
                     L"
BASE COUNT          616 a          273 c          209 g          648 t
ORIGIN      
        1 gtagtttatg aaatttgggg aagttttata atgttcggaa tttgtttgat tccttttgtc
       61 attgctttct taatgatttc tcgttctcat tgttttgatg aaatcgataa acataaaggt
      121 catattgcat aagtttatgg atagatagtt atatgctatc tatccatttt tagattaaat
      181 taaaatattt tcttttaaat aatataattt atattataat gatattcatg gagaagttta
      241 tgaagaatat cacaaaaatg tttttattta tattatcgtt ctacttagtc ttaggttccc
      301 tttggattgg aaaagatgtt atcattcatt atcaaaatat tgatggttta ccaatcctag
      361 gttatcatgg tgtcctcgaa gataaagata aagaaaaata ctttgctaat tatccatact
      421 gtatgtcatt gagtgaattt gaagctcaga tgaagtattt aaatcaaaat aattatcata
      481 ccttaacaat ggatgaaatc aacgattatt atcaaaatca tacaccacta cccaaaaaaa
      541 gtgttgctct tacctttgat gatggcttat taaactttaa aacagttgtt aaacctattt
      601 tagaaaaata taattttaaa gctacttgtt ttgttattgg ctataaaact acagtaaaaa
      661 atagccaaaa cccttataag catcaatact taagaaaaag tgatttagta aatgacgaat
      721 atgttgaata ctattcacat tcttataatc ttcatcataa cactaaatat cctaatacca
      781 aactaattga aacattatcc acacaagaaa taatcaatga ttttaaacaa aatgaaaata
      841 ttgtttctag taaatacttt gcctttccat atggtcgaac atgtgacaat gctaatgaag
      901 cattgataaa agctaatgtt tcattagctt ttggttataa ccaaaataga acgatgacct
      961 atcatgatga taagtacttg ttaccacgat atctaatgtt tagtaaaatg ccaatgttct
     1021 attttaaatg gatcgttgaa taaaaaatga caaggactgt ttgttcttgt catttttata
     1081 attattataa gacttttcca ttactagcaa taaccttttg ataccaataa aaactatctt
     1141 ttaaatatct ctttccactt ccatttccat aatcatcgta gtcaacataa atgtaaccat
     1201 atcgcttatt catctctgaa gtacctgcac ttatcatatc aattggtgac cagctcaaat
     1261 atcctaagca tatgactcca tccaactcaa ttgccttctt taactcatta atatgttttt
     1321 gaagatactt aattcgatat tcatcatgaa tctgattgtc tacagtcaaa atatcattag
     1381 caccaaaacc attttcacta atcaaaatag gtaaattata acggtagtat atctcattca
     1441 ttgtaattct taaaccaatt ggatcaatag cccaacccca atctgatttt tccaaatatg
     1501 gattttcaac accacctttt aaattacctt cccctacttt tctagcaggg tcatgagatg
     1561 ctactaatga taaataataa ctaaaagata tgtaatcaac tgtattattt ttgattatgt
     1621 ctaattcttc ttttgagaaa tttaaatgaa tatctctttt ttctaaattt tttaaatacc
     1681 agttaggata attcccatta gcctgaacat ctgcgtataa ataatattct tgatttgctc
     1741 tcattg
//
LOCUS       DAOMTA010000710         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3509_length_1743_cov_2.100711, whole genome shotgun sequence.
ACCESSION   DAOMTA010000710 DAOMTA010000000
VERSION     DAOMTA010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_1743_cov_2.100711"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..905)
                     /locus_tag="PLK68_14770"
     CDS             complement(141..905)
                     /locus_tag="PLK68_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91712.1"
                     /translation="MLINQLDDEIIKNLSQSELYILHYVYDHPDEVIDMSIQELAKAV
                     AFSSATILRFCKKLNFSGFAEFKFALKQQNKEIANLKKPISSMDSITSLYDDIESTSL
                     LIKERYLKEIIDLIDSNKRIHLYGEGISRIPVDYLEKLLFSIGRQNVYKYKSSRLAAH
                     IASNANEDDILIAISTSGNYPTTVKIVKLFNLNHATIIAISPYTKNAIADLANINFRF
                     FVNHRENIDTEYTSRLAIFYIIDVIFKTYLVRKEKE"
     gene            962..1441
                     /locus_tag="PLK68_14775"
     CDS             962..1441
                     /locus_tag="PLK68_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM91713.1"
                     /translation="MGLFKKKDKYLKAFTSGKIIPITEVPDEVFAQKMMGDGIAIYPD
                     NNIIVAPCDGKITAIMENTEHAVGITLANGVELLIHVGLDTVNLTEKIFSVKVAKESY
                     VHTGEELITFDKVRLKELGYQDITMLVILDTGNTKKLEILSETEAIINQTNIIKYID"
BASE COUNT          698 a          222 c          239 g          584 t
ORIGIN      
        1 attagacata tacgttgctt ttgctaactg tgataatgtc attgatacaa cttccttaca
       61 actattttca agataatata gtaactcttt ttcattttca gacaaatcta aagatttagc
      121 attttcaagt aaagcaatca ttattccttt tcctttctaa ctaaataagt tttaaatatt
      181 acatcaataa tatagaaaat tgctaatctt gaagtatatt cagtatcaat attttcacga
      241 tgattaacaa aaaaacgaaa attaatattt gctaaatcag caatagcatt cttagtatat
      301 ggactaattg ctataattgt tgcatgattt aaattaaata atttaacaat cttaaccgta
      361 gtgggataat taccactagt acttatagca attaatatat catcttcatt ggcattacta
      421 gcaatatgtg ctgctaaacg agaagattta tacttgtaaa cattttgacg accaattgaa
      481 aataacaatt tttcaagata gtcgacaggg attcgggaaa taccttcacc atatagatgg
      541 attcttttat tactatcaat caaatcgatg atttctttta aataacgctc tttgattaat
      601 aatgaagtgc tctcaatatc atcatataga ctagtaatag aatccattga agaaattggt
      661 ttctttaaat tcgcaatttc tttattttgc tgttttaatg caaacttaaa ttcagcaaaa
      721 ccactaaaat taagtttctt acaaaaacga agaatcgtag cagaagaaaa ggcaacagct
      781 ttagctaatt cttgaatact catgtcaata acctcatcag gatgatcata aacataatga
      841 agaatatata attcactttg acttagattt ttaattattt catcatctag ttgatttatt
      901 aacataagtt tcctcttttc tttcatatag taaaataatt atacaataaa ggagtaaaaa
      961 aatgggatta tttaaaaaaa aagataaata tttaaaagcc ttcaccagtg gtaagatcat
     1021 tccaattaca gaagtaccag atgaagtatt tgcacaaaaa atgatgggcg atggaattgc
     1081 aatctaccct gataataata taatcgttgc accatgtgat ggtaaaatca cagctattat
     1141 ggaaaataca gaacatgcag ttggaatcac attagcaaac ggtgtagaat tattaattca
     1201 tgttggtcta gatactgtta atctaacaga aaagattttt tcagtaaaag tagctaaaga
     1261 aagctatgta catacaggtg aagaattaat tacttttgat aaagtaagac taaaagaatt
     1321 agggtatcaa gatattacaa tgttagtaat tttagatacg ggtaatacta agaaactaga
     1381 aatactatca gaaacggagg caattataaa tcaaacaaat atcattaaat atatagatta
     1441 aatattttat tgaaaacaat aaaaaataat aataaatata gataatatcc aagggtaatg
     1501 atatactatg tactagcgaa tcctgttgga ttttatcgaa aataaaatga aacaactgaa
     1561 atgaaaaaat aatatttgaa gagggagatc atgctaactg cattaattta tagcatagct
     1621 ctctttttta tgttatcaag atataaaatt aggttgtgat aaaaaaagtt agaccaatct
     1681 ttagataaat ttaattttgt agataaaatc agtgtatatt gaagttgata agtaaagaaa
     1741 gat
//
LOCUS       DAOMTA010000711         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3513_length_1741_cov_2.392645, whole genome shotgun sequence.
ACCESSION   DAOMTA010000711 DAOMTA010000000
VERSION     DAOMTA010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_1741_cov_2.392645"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PLK68_14780"
     CDS             <1..966
                     /locus_tag="PLK68_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM91714.1"
                     /translation="TAIVSVKISEPQFEGQTKSKLGTPEARNVVDTVVYEKLTYFLEE
                     HKETADNLVKKMIKAAQVRDAARKAREAARKGKGPRQEKILSGKLAPAQSKDKKRKEL
                     YLVEGDSAGGSAKQGRDRKYQAILPLRGKVVNTEKAAMADILKNEEIATIINTIGAGV
                     GADFNEKDSNYNKVIIMTDADTDGAHIQILLLTFFYRYMRDLITAGKVYIALPPLYKV
                     SKKSGKKEDIVYAWEEDELEEAKKKIGRGYNVQRYKGLGEMNASQLWETTMNPETRTL
                     IQVSIEDAAIVERRVSVLMGDKVEPRREWIEQNVQFTLEDNFAIE"
     gene            998..>1741
                     /locus_tag="PLK68_14785"
     CDS             998..>1741
                     /locus_tag="PLK68_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM91715.1"
                     /translation="MSLETIMGDRFGKYSKYIIQDRALPDVRDGLKPVQRRILYSMYK
                     EGNLYSKPYRKSAKTVGNVIGNYHPHGDKSVYDAMVRLSQDWKMRVPLVDMQGNNGSI
                     DNDPAAAMRYTEARLSAIAVELLKDLDKDTVLMSLNFDDTEYEPTVLPAAYPNLLVNG
                     ATGISAGYATDIPPHNLEEVIDATIHRIKYPNCSLEKLMEFIKGPDFPTGAIVEGKQG
                     ILNAFKSGRGKVIVRSTSEMIEEKTMNKIV"
BASE COUNT          647 a          253 c          359 g          482 t
ORIGIN      
        1 acagcgattg taagtgtcaa gatttcagaa cctcagtttg aaggacaaac aaaatctaaa
       61 ctaggaacgc cagaggcacg aaatgttgta gatacagtag tttatgaaaa acttacatat
      121 tttttagaag agcataaaga aacagcagat aatcttgtta agaaaatgat taaagctgct
      181 caagttcgtg atgcggcacg taaagctcga gaagctgctc gtaaaggtaa aggcccacgt
      241 caagaaaaaa ttttaagtgg aaaattagca cctgctcaat ctaaagataa aaaacgtaaa
      301 gagctatact tagtcgaagg agattcagca ggcggtagtg caaaacaagg tcgcgatcgt
      361 aaatatcaag caattttacc attacgagga aaagttgtta atactgaaaa ggcagcaatg
      421 gctgacattt taaaaaatga agaaattgca acaattatta atacaattgg ggctggcgtt
      481 ggagctgatt ttaacgaaaa agatagtaat tataataaag taatcatcat gaccgatgct
      541 gataccgacg gtgcccatat tcaaattctt ttactaactt tcttctatcg atatatgcga
      601 gatctaatta ctgcaggaaa agtatatatt gctttaccac cactttataa agtgtcaaag
      661 aaaagtggga aaaaggaaga tatcgtttac gcttgggaag aagacgaatt agaagaagca
      721 aagaagaaaa ttggtcgtgg atataatgta caacggtata aaggacttgg ggagatgaat
      781 gcttctcaat tatgggaaac aactatgaat ccagaaactc ggactttaat tcaagtatca
      841 attgaagatg cggcaattgt tgaacgtcga gtttctgtat taatgggtga taaagtagaa
      901 ccaagacgag aatggattga acaaaatgtc caatttacat tagaagataa ttttgcaatt
      961 gagtaaggag aaaataagcg tggataaaaa aataattatg tcattagaaa ctatcatggg
     1021 tgaccgcttt ggaaaatatt ctaaatatat tattcaagat cgtgctttac ctgatgttcg
     1081 tgatggttta aaaccagtac agcgccggat tctttattcg atgtataaag aaggaaattt
     1141 atattcaaaa ccatatcgta agtcagcaaa aacagttggg aacgttattg gtaattatca
     1201 tcctcatgga gacaaatcag tatatgatgc aatggtccgt ttatcccaag attggaaaat
     1261 gcgtgttcca ttagttgata tgcaaggaaa taatggatct attgataacg atccggcagc
     1321 agcaatgcgt tatactgaag ctcgtctatc agcaattgct gttgaattac taaaagattt
     1381 agataaagat acagtattaa tgtctttaaa ctttgatgat acagaatatg aaccaactgt
     1441 attaccagcg gcctatccaa atttattagt aaatggtgca acgggaatat cggccggtta
     1501 tgcaacagat attcctccac ataatcttga agaagtaatc gatgcaacga ttcatcgaat
     1561 taaataccct aactgttctt tggaaaaact aatggaattt attaaaggtc cagattttcc
     1621 aactggagct attgttgaag gaaaacaagg aattttaaat gcctttaaaa gtggccgtgg
     1681 gaaagtgatt gttcgtagta ctagtgaaat gattgaagag aaaacgatga ataaaatcgt
     1741 t
//
LOCUS       DAOMTA010000712         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3522_length_1739_cov_2.356295, whole genome shotgun sequence.
ACCESSION   DAOMTA010000712 DAOMTA010000000
VERSION     DAOMTA010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_1739_cov_2.356295"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLK68_14790"
     CDS             <1..368
                     /locus_tag="PLK68_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM91716.1"
                     /translation="ATMRRLKYLEHQEIEDVVFTNGDLKVNFDSGCAYLNDQELHLTP
                     IEYKLLCLLAKNIDKVLTHNYILKEVWGDVIQSDVPSLRVFMATLRKKIESDTTNPKY
                     IQTHVGVGYRMLRVEREKE"
     gene            413..>1739
                     /locus_tag="PLK68_14795"
     CDS             413..>1739
                     /locus_tag="PLK68_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM91717.1"
                     /translation="MKNKDNYVNLTKIIVIWILATISSLILDYFKIRVENILLIYVVG
                     VLIIYIMTFNYLYTDPRYTFLINDPNYLISVFIFIIVAIIVSTLTNRLKKQREIALYQ
                     EEVTSKINQISSGFLNLSGYEEIRTYCQDSLYNLTKIKNEVFLYQNKEFQDLMAWWCY
                     CHGEPCGKDQKKFTYLKEVYLPIKKDNYTYGTIKFDCNQRTITDEDLIYIKTIIAELI
                     LVLQRDLLSHEKEEARLQVEREKLKSTLLRSISHDLRTPLTSIAGGANFLVNNLDTVE
                     SDTSLNIIQDISKEAMRLNGMVENLLNMTRIQEGNFKINKKLEVVDDIISTAVSAITN
                     RKENHELVVKETKDIILFPCDAQLIVQVLVNLLDNAFKHTPDASKVMLKAFVRNNNII
                     FQVIDNGKGIEIKQLNHIFDDFFTTSLDNGDHKRGIGLGLAICKAIVEAH"
BASE COUNT          656 a          191 c          298 g          594 t
ORIGIN      
        1 gggcaacgat gcgaagatta aaatatcttg aacatcaaga aattgaagat gttgttttta
       61 ctaatgggga tttaaaagtt aattttgatt ctggctgtgc ctatttaaat gatcaagaat
      121 tacacttaac ccctatcgag tataagttgt tgtgtttatt agcaaaaaat attgataaag
      181 ttttgactca taattatatt ttaaaagaag tatggggtga tgtaattcaa agtgatgtcc
      241 catctttacg agtttttatg gcaactctac gaaagaaaat agaatcagat acaactaatc
      301 ctaaatatat tcaaactcat gtaggggttg gttatcgaat gttgagagta gaaagagaga
      361 aagagtaaat cactttctct tgaatcattg agtagcaggc taggaggtta atatgaagaa
      421 taaggataat tatgttaact taactaaaat cattgttata tggatattag caacaatttc
      481 atcattaata ttagattatt ttaaaataag ggttgaaaac attcttttaa tttatgttgt
      541 tggagtacta ataatttata ttatgacttt caattatctt tataccgatc ctcgatatac
      601 ttttttgatt aatgatccta attatttaat atctgttttt atttttatta ttgttgcaat
      661 aattgtttct acattaacaa atcgactaaa aaaacagcgt gagattgctt tgtatcaaga
      721 agaagtgaca tctaaaataa atcaaattag tagtggcttt ttaaatctat cgggttatga
      781 ggaaataagg acttattgtc aggacagttt atataattta acgaaaataa aaaatgaagt
      841 atttctatat caaaataaag agtttcaaga tttaatggca tggtggtgtt attgtcatgg
      901 tgaaccatgt ggaaaggatc aaaagaaatt cacatattta aaggaagtat atttaccaat
      961 caaaaaggat aattatacgt atggtacgat caaatttgat tgtaatcaaa gaactattac
     1021 tgatgaagat cttatttata taaaaacaat aattgcggag ttgattttag tattacaaag
     1081 agatctttta agccatgaaa aagaagaagc tagattacaa gtggagcgag aaaaattaaa
     1141 aagtacttta ttaagaagta tttctcatga tcttagaaca ccattaacta gtattgctgg
     1201 tggggctaat tttttagtaa ataatttgga tacagttgag tctgatacta gtctaaatat
     1261 tattcaagat attagtaaag aagcaatgcg tttaaatggg atggttgaaa acctattaaa
     1321 catgacaagg attcaagaag gaaattttaa aataaataaa aaactagaag tagttgacga
     1381 tattatatct acagcagttt cagcaattac taatcgtaaa gaaaatcatg aattagttgt
     1441 taaagaaacc aaagatatta ttttgtttcc ctgtgatgct caattaattg ttcaagtact
     1501 cgttaattta ttagataatg cttttaaaca tactcctgac gctagtaaag tgatgttaaa
     1561 agcatttgtt cgaaataata atattatttt tcaagtaatt gataatggta aaggaattga
     1621 aataaagcag ctcaatcata tctttgacga tttctttaca accagtctag ataatggtga
     1681 tcataaacgt ggaattgggc taggtctagc aatatgcaaa gcaattgttg aagcgcata
//
LOCUS       DAOMTA010000713         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3525_length_1739_cov_1.679929, whole genome shotgun sequence.
ACCESSION   DAOMTA010000713 DAOMTA010000000
VERSION     DAOMTA010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_1739_cov_1.679929"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLK68_14800"
     CDS             <1..1340
                     /locus_tag="PLK68_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91718.1"
                     /translation="IDRLSIFKIAFGLQLPFENKSVFSYQSLFNRVFNQRYCLKRADE
                     MTFSYCLKNEKPYAEALQIIISYKRAEKEFLINRKTQEKNVPVESTKHLFYTVLSAKG
                     TDELIEILIHNIPSFISEKTSVNDELNSRAAVFNNEDVMKSFNQRYDTIKSALTIKEM
                     YFSIIEESRDDYDRKQFEKDIETKYPNFYNYVVVSDGIFDNAQKQLALVNYLLRHPEK
                     LELFINEDDALEEQKNSLRTDEYYNAVGCPEFLRCINTKEDITAQLENIKADLSLILE
                     IVEPMHFQKLKLEKKDKVNKGEQTNHADIKLNIMSESIKPDSVPREVFVDFSAKTSSK
                     NSNYYESIRKLIVIISFFNYFNDYIGESYSKEEFREIADGFITEINHILSYYNYNELY
                     AYNTFDLFFFLCIHTTDPIYTYYLIIRYYTDKYYILNRPDRDLEAFIKKYKIK"
BASE COUNT          632 a          289 c          305 g          513 t
ORIGIN      
        1 gtattgatcg attgagtatt ttcaaaattg cctttggctt acaacttcct tttgaaaata
       61 aatccgtttt ctcctaccag tcgctgttta accgggtctt taatcaaaga tattgtctca
      121 aacgagcaga tgaaatgaca tttagctatt gcctaaaaaa tgaaaaacct tatgctgaag
      181 cgttacagat tatcatttcc tataagagag ccgaaaaaga atttctaatt aaccggaaaa
      241 cgcaagaaaa aaatgttccc gttgaatcga ccaagcatct tttttacact gtattgagtg
      301 cgaaaggtac ggatgagtta attgaaatcc tcattcataa tattccctcg tttatcagcg
      361 agaaaacctc tgtaaatgat gaactcaatt cacgagcagc tgtttttaat aacgaagacg
      421 taatgaaaag ctttaaccaa agatatgata caataaagtc ggcattgacg ataaaagaga
      481 tgtacttttc tatcattgag gagtccagag atgattacga tagaaaacaa tttgaaaaag
      541 atattgaaac aaaatatcct aatttttata attatgtagt tgtatcagac ggtatttttg
      601 ataatgcgca aaaacaactg gcgttagtca actatttatt gcgtcatccc gaaaaattag
      661 aactcttcat caatgaagac gatgcattag aagaacaaaa aaattctctt agaacggacg
      721 agtattacaa cgccgttgga tgtcctgagt tcttgcgatg tattaatact aaggaagaca
      781 taactgctca actggaaaat atcaaggcgg atttatccct tattctggaa attgtagaac
      841 caatgcattt tcagaaactg aaacttgaaa aaaaagacaa agttaataaa ggggagcaaa
      901 caaatcatgc tgatattaaa ctaaacatca tgagtgagtc aataaaaccc gattcagttc
      961 cccgcgaagt gtttgtggat ttcagtgcga aaacatcctc aaaaaacagt aattactatg
     1021 agagtatcag aaaactgatt gttataattt cctttttcaa ttacttcaat gactatatcg
     1081 gtgaaagcta ttccaaagaa gaatttagag aaatagccga tggttttatt acagaaatta
     1141 atcatatttt atcttattac aattacaacg agttgtacgc gtataacact tttgatttat
     1201 tcttctttct ttgtatccat accaccgatc cgatttatac ctattatctg attatcagat
     1261 actacacgga taaatactat attctaaacc gccccgatcg agatctggaa gcgttcataa
     1321 aaaaatataa aattaaataa atgtacacta caaggcatcc acagaaatgt gggtgctttt
     1381 ctttttggac gaaatttaaa aaatcatgtc tttccccaga tatataatat aagtacacta
     1441 aagaaaggag attacgctaa ctcatccgct gaaaaatata agacaataga tatggaggtt
     1501 cgattatgag caaaagcaga cagtacaccg catgggacaa ttcagacaga gaagattttt
     1561 atgatgacta cggcaatctt cagactactc atgtagacag atacaattta agtggcgaat
     1621 acactcgcac ggatgtctat aatgagtatg ggaagaagat tgatagtttc aaacgctaat
     1681 ttctatcacg tggataagga ggtgaaattg aatgtggcat agtttctggg aagctatgg
//
LOCUS       DAOMTA010000714         1738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3527_length_1738_cov_1.884135, whole genome shotgun sequence.
ACCESSION   DAOMTA010000714 DAOMTA010000000
VERSION     DAOMTA010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3527_length_1738_cov_1.884135"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..620
                     /locus_tag="PLK68_14805"
     CDS             105..620
                     /locus_tag="PLK68_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRM91719.1"
                     /translation="MKERETKFDIIAEYYQSHYVELIAFVGKRIDYTDEVEDIVQNIF
                     VRLLLSDKMISEITLPCLVYTVARNLIYDFWRHHRLINEYEHYFKSTTSCGNNDTLSI
                     YSANEINQILEKGIALLSDKKRHIYKMCIYDGMKVSEISKTLGENYKRVENYLGAARK
                     EVRQYVDKMLA"
     gene            807..893
                     /locus_tag="PLK68_14810"
     tRNA            807..893
                     /locus_tag="PLK68_14810"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:841..843,aa:Ser,seq:gct)
     gene            915..986
                     /locus_tag="PLK68_14815"
     tRNA            915..986
                     /locus_tag="PLK68_14815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:948..950,aa:Glu,seq:ctc)
     gene            complement(1081..1557)
                     /locus_tag="PLK68_14820"
     CDS             complement(1081..1557)
                     /locus_tag="PLK68_14820"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRM91720.1"
                     /translation="MKKVSLLFVCLGNICRSPAAEGITKKIAEDKGILTLLDIDSAGI
                     GDWHIGQLPDARLRKHGYIHGYKFNSKARQICMNDFNSFDYIIAMDKDNYSYLSNIAP
                     NKECKKKILRISDYLKLYPNYDKIPDPYYGDDDDFENVIILLEDACKNLLLSLFNK"
BASE COUNT          557 a          269 c          295 g          617 t
ORIGIN      
        1 atttatgtat cattttttat tatgcgttcc ttttttgaat gctttaagtt gcatcatttg
       61 ttttttaata ttatctttgc aatactaaaa acacaaattg aaacatgaag gagcgcgaaa
      121 ccaaatttga cattattgca gaatactatc aatctcatta tgtagaattg atagcattcg
      181 ttggtaaacg tattgactat actgatgaag ttgaagatat cgttcaaaat atatttgtac
      241 gtctactttt atcagacaag atgatatccg aaataacatt gccttgtctt gtttatacag
      301 tggccagaaa tcttatttat gatttttggc gccatcaccg tttgattaat gaatacgaac
      361 attattttaa gtcaacgaca tcatgtggca acaatgatac attatctata tattcagcaa
      421 atgagataaa ccagatatta gaaaaaggta tagctcttct atctgataaa aaacgtcata
      481 tatataaaat gtgcatatac gatggaatga aggtttctga aatatcaaag acgttgggcg
      541 aaaattataa gagggtggag aattatcttg gagcggcaag aaaagaagta cgccagtatg
      601 ttgacaagat gttggcatag ggaggatatt attatgttat attcatttat tggctaatgt
      661 atattgtcat ttgtgatgga ttagataacc tatgatttta atcttggcac tttttaaaaa
      721 aaaatcaatg aaaagtttgt gtaatcaaaa ataatccttt acctttgcac tcgcatttga
      781 gaaaagtgtt gcggtttgta ccaaaaggaa gtttgggtga gtggctgaaa ccagtagttt
      841 gctaaactgc cgaacgggta accgttccgg gggttcgaat cccccagctt ccgcacactt
      901 ttgcaactag ccacggtggg ttcatctaat ggttaggata caagattctc aatcttggca
      961 tacgagttcg attctcgtac ccactacgga tgaaataatt aagtagctga ttttcagcta
     1021 ctttttttat gccttgatgg attatatatt gctttagcct ttattttgaa taaagaagac
     1081 tcatttattg aataacgaaa gtagtaaatt tttgcatgca tcttcaagaa ggataatcac
     1141 attttcaaaa tcatcatcgt ctccataata tggatcagga atcttgtcat aattagggta
     1201 taacttcaaa taatcgctaa tacgtaaaat cttcttttta cattctttgt taggagcaat
     1261 atttgataag tatgaataat tatctttatc cattgcgatg atatagtcga aactgttaaa
     1321 atcgttcatg catatttgcc ttgccttact attgaatttg tatccatgta tgtatccatg
     1381 ttttctcaac ctagcatcag gaagttgtcc tatgtgccag tctccaatac cagcggagtc
     1441 tatatctaac agtgttaata ttcctttgtc ttcagctatt ttttttgtta taccctcagc
     1501 agcgggagaa cggcatatat ttcctaagca tacaaacaac aacgatacct ttttcatata
     1561 tatagcttct tttatttctt tttattatta ttgctgcaaa ggtaataaaa ttattattgc
     1621 aaaagaacaa taataaataa tgtatgttcg ctgatagtgt aaaaaaatcc aagccattat
     1681 gctagcttgg attcttatta ttgatatttt ttaataacgt taccgtattt atgctgat
//
LOCUS       DAOMTA010000715         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3546_length_1733_cov_2.075089, whole genome shotgun sequence.
ACCESSION   DAOMTA010000715 DAOMTA010000000
VERSION     DAOMTA010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_1733_cov_2.075089"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1490
                     /locus_tag="PLK68_14825"
     CDS             249..1490
                     /locus_tag="PLK68_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM91721.1"
                     /translation="MNKILMFVEDKLVPPLNKMANQHHLNAVKNGMMVTVPLTIIGSI
                     FLLIPNIPIDPIQSFFEPYAAMITTVNTITIGIVGLVGAASVAYYFALGYTDIKIDPL
                     ITAFVSVAAFLLATLTDEYAINLELFGTKGLFTAILVALMSGMIMHFFQKRDLVIHFP
                     DTVPPLVSKSFMSLVPAFVILTIIWIIRVILGINVNQILMDCFSPFVFALNTLPGFLV
                     FMFIRSMLWSVGIHGGAVLAVADPFFLTMFGANAAAFAAGTQPPYITASGFTVFVFLG
                     GGGATLPLVLMMIRSKEKGFSTLGKLCLPASIFEINEPVVFGVPLVMNPYMMIPYTLS
                     TLILSAGTYLLMLFNIIGRPVANIPWTIPPLFSHYLVTGGNIPAVIWGGISLLIAGCI
                     YYPFFKAMERQRLAVEKRIGA"
     gene            1493..>1733
                     /locus_tag="PLK68_14830"
     CDS             1493..>1733
                     /locus_tag="PLK68_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM91722.1"
                     /translation="MNKKLKIAIVGAGSSYTPELIEGLSEHRETLPVKEIVLLDINEE
                     RLSIMEGFVKRYVGYLNYDVKVWSTTSREEAFTDAD"
BASE COUNT          560 a          231 c          324 g          618 t
ORIGIN      
        1 taattagtac tttttaagat gggagtttca atatataatt gaaactcttt tttattttca
       61 tattagtatg aattgaggaa aaataaatta gtaagtaaga agatatatag aagaaagata
      121 aaataatttt aattatgcaa ttatttccac tagccaaggg aaattttaac tattcaaata
      181 aacatacttt ctctttatta ttagattgta caaagaagag cgctcgtgga aatgagagga
      241 gaaaaataat gaataaaata ttaatgtttg ttgaggataa actagtacca ccattaaata
      301 agatggctaa tcagcatcat ttgaatgcag tcaagaacgg tatgatggtt acagttccat
      361 taacaattat cggaagtatc tttttgctaa ttcccaatat tccaattgat ccgattcaat
      421 cattttttga accatacgct gctatgatta caacagttaa tacaataacg attgggattg
      481 ttgggctggt tggagctgct agtgtagctt attattttgc tttaggctat acggatatta
      541 aaattgatcc attaattaca gcatttgttt ctgttgccgc gtttttattg gcgacactaa
      601 cagatgagta tgcgattaat cttgaattat ttgggacaaa aggattattt acagctattt
      661 tagtagcatt aatgtcagga atgattatgc atttttttca aaagagagat cttgtgatac
      721 attttcctga tacagtacca cctctagtat caaaatcatt tatgtcattg gttccagcat
      781 ttgtcatttt gacaattata tggataattc gtgtaatttt aggtattaat gttaatcaaa
      841 ttttaatgga ttgtttctca ccatttgttt ttgcattaaa tactctaccg ggattcttgg
      901 ttttcatgtt tattcgctca atgttatgga gtgttggtat tcacggtgga gctgttttgg
      961 ctgtagctga tccatttttt ctaactatgt ttggagcaaa tgctgctgct tttgcagctg
     1021 gaactcagcc accttatatt actgccagtg gatttactgt gtttgttttc ttaggtggcg
     1081 gtggtgctac tttaccgctt gtcttgatga tgatccgttc aaaagaaaaa ggattcagta
     1141 cattaggaaa attatgttta ccagcaagta tatttgaaat taatgaacca gttgtttttg
     1201 gggtaccatt agttatgaat ccatacatga tgatacccta tactttgtca acattaattt
     1261 tatcagcagg aacatatcta ttaatgctct tcaatattat aggaagacca gtagctaata
     1321 ttccatggac aataccacca ttattttcac attatttagt tactggtggg aatattcctg
     1381 ctgttatttg gggaggtatt tcactattaa tagcaggatg tatatattat ccattcttta
     1441 aagcaatgga aagacaacgt ttagcagtag aaaaaaggat aggagcatga gtatgaataa
     1501 aaagttaaaa attgcaattg ttggagcagg gagttcgtat acccctgaat taattgaagg
     1561 attatcggaa caccgtgaaa cattaccagt aaaagaaatt gttttattag atataaatga
     1621 agaaagactt tcaattatgg aagggtttgt aaaaagatat gttggttatt tgaattatga
     1681 tgtgaaagta tggagtacaa catcaagaga agaagcattc actgatgctg att
//
LOCUS       DAOMTA010000716         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3566_length_1725_cov_2.220958, whole genome shotgun sequence.
ACCESSION   DAOMTA010000716 DAOMTA010000000
VERSION     DAOMTA010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_1725_cov_2.220958"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLK68_14835"
     CDS             <1..238
                     /locus_tag="PLK68_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91723.1"
                     /translation="CLRTDNIQKTTLLLEQKGKINNYSVYPDNSIRIYECLENVRMIS
                     KLLSSNGVIIDEISVQGENLETYFENLIGGRKNV"
     gene            231..>563
                     /locus_tag="PLK68_14840"
     CDS             231..>563
                     /locus_tag="PLK68_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91724.1"
                     /translation="MFNQIRAEFYKLFHTKALYLTFALILAVFGIFSIGGQQQFVVSS
                     SSLDGTWKIGETVGFLARAYSDTAHPMIEEIIRTATSYTVFFWLIVLIFSVIFFSREY
                     TDSTIKIAI"
     assembly_gap    564..573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <574..1055
                     /locus_tag="PLK68_14845"
     CDS             <574..1055
                     /locus_tag="PLK68_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91725.1"
                     /translation="QSRIKFFVAKYIVITITSIFLYFSFIMIAFIIECAKFNVPIQLF
                     PMLKIAGLNCMVMGAFIGITLMLCVIFKHTAIVVGAMSLFTFSGPLIYMMTWDNMSVQ
                     SWRVLTYLKINPMYYWMNTCSYNMINHLEINILTYFVGTVIITFLVSALILRKQEIR"
     gene            1196..>1725
                     /locus_tag="PLK68_14850"
     CDS             1196..>1725
                     /locus_tag="PLK68_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM91726.1"
                     /translation="MSLSNKYIEELAYAINQKDTLHKKLQVQIKQEEEQLKQSISNIS
                     HDLRTPLTSIQGYLTLLQECENKQEQEQYLKIIKAKTDYLTELVQYFYDLSVLENEQS
                     DVECERIDINRIVTDCLIEKYYEFGEIQPIIQTENSPVWIYGNNLICKRIIENLIVNA
                     LRYSDNYIEVSINQKGV"
BASE COUNT          617 a          232 c          287 g          579 t
ORIGIN      
        1 ttgtttaagg acagataata tccaaaaaac cactctttta ttagagcaaa agggaaaaat
       61 taacaattac tccgtttacc ctgataattc tattcgtata tacgagtgct tagagaatgt
      121 aagaatgatt tctaaattgc tatccagtaa tggagttatt attgatgaaa tttctgtgca
      181 aggagaaaat ttggaaacat attttgaaaa tctaataggg ggacgaaaaa atgtttaatc
      241 aaataagagc agaattttat aaattatttc atacaaaggc attatactta acctttgctt
      301 tgattttagc ggtctttgga attttctcca taggcggtca gcaacaattt gtggtatcaa
      361 gttcaagttt agacggaaca tggaaaatag gagaaacggt tggatttctt gcacgtgctt
      421 atagcgatac agcacaccca atgattgaag aaataataag gacagccaca tcttatacgg
      481 tatttttctg gctgattgtt cttatatttt ctgtcatttt cttttcaaga gaatatacgg
      541 attcaacaat taaaattgcg attnnnnnnn nnngacaaag ccgaattaag ttttttgttg
      601 cgaaatatat tgtgattacg attaccagta tatttctata cttttcattt attatgattg
      661 catttataat agaatgtgcg aaatttaatg ttccaataca actgtttcca atgctgaaaa
      721 ttgcaggact aaattgtatg gttatgggag catttatcgg aatcacactt atgttatgtg
      781 taatattcaa acatacggca atcgttgtag gtgctatgtc attgtttaca tttagtggac
      841 cgcttatcta tatgatgaca tgggataata tgtcggttca gtcatggaga gttctaacct
      901 acttaaaaat aaatcctatg tactactgga tgaatacttg ttcttacaat atgataaatc
      961 atttagaaat caatattctg acttattttg taggaactgt aattattaca tttcttgttt
     1021 cagcattgat attaagaaag caagaaatac gctaatcata acagaaaggg gaaatgcgaa
     1081 tatgttatat tttttattga tttgtcttat tttcatatta gtattttccc tattctctat
     1141 tatcagaacc attcataata taaataagca aataaaggaa cagcggaaaa tacgggtgtc
     1201 cttatcaaat aagtatattg aagaattagc ttatgcaatc aatcaaaaag atactctaca
     1261 taaaaaattg caggttcaaa ttaagcagga ggaagaacag ttaaaacaat ctatatctaa
     1321 tataagccat gatttaagaa cacctttgac atctatacaa ggctatttaa cacttttgca
     1381 ggaatgtgaa aataaacagg agcaagaaca atatctgaaa atcattaaag caaaaactga
     1441 ctatctcaca gagttagtgc aatactttta tgatttgtct gtgttagaaa atgagcaatc
     1501 ggatgttgaa tgtgagcgta ttgatataaa tagaattgtt acagattgtt taattgaaaa
     1561 gtattatgaa tttggagaaa tccagccaat cattcaaaca gaaaattctc ctgtatggat
     1621 atacggaaat aatcttatct gtaaacgaat tattgaaaat ttaattgtaa atgcacttcg
     1681 ttattcagat aactacattg aagtttctat caatcaaaaa ggagt
//
LOCUS       DAOMTA010000717         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3576_length_1724_cov_2.288197, whole genome shotgun sequence.
ACCESSION   DAOMTA010000717 DAOMTA010000000
VERSION     DAOMTA010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3576_length_1724_cov_2.288197"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..626
                     /locus_tag="PLK68_14855"
     CDS             405..626
                     /locus_tag="PLK68_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HRM91727.1"
                     /translation="MDPELIVRINELAKKKKDGTISEAELEEQKELRQKYLKAFRSGF
                     KQQLMGVKVVDEEGNDITPQKLKDEKLKN"
     gene            718..>1724
                     /locus_tag="PLK68_14860"
     CDS             718..>1724
                     /locus_tag="PLK68_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRM91728.1"
                     /translation="METSSLSIATIRALGIDTINKANSGHPGMVLGSTPAIYTLFTKE
                     MDFYHKQSKWFNRDRFVLASGHASALLYSMLHLSGYQISIDDLKNFRQWGSITPGHPE
                     CDITDGVDASSGPLGQGIPMAAGMALAEKFLASKYNREGYDVVDHYTYALCGDGDMQE
                     GVTYEAASLAGHLSLGKLIVLYDANKVTLDGPLSMSFSEDVKKRYEAIGWQVIRVTDG
                     NDVSAIQKAIRKGKKELYKPTLIIIDTVIGFGSSNEGTNKVHGNPLGKEDGKNAKISY
                     GFDHDEFYVPEEVYDDFANTCIKRGKNRYNKWQRLMKDYKNAYPELAQELENAIAGNF
                     S"
BASE COUNT          608 a          216 c          337 g          563 t
ORIGIN      
        1 tattgcgaat ttattcagtc gctattattt tgaaggaact atcgctttga atttagtcga
       61 tgaggtggct gatattcaac ttaaagatat ttataacact tttaaatatt ttgatattga
      121 atacactagt acttgtattg ttaagaaaaa gtaataattt tgtcacatat cgctatgctt
      181 aaagtgttga cacttaaggt ggctgtgtta taataatttt ggttagagag taaataaatt
      241 tccacatcat agataaggga acctgatcta aattgcggtg ttgaaaacct gtctttgaat
      301 agggatccta aagtatattt aaaggtgccc acctgtactt aacagggaaa tatctaaagc
      361 tctctacctt ttttatattt aaggagaaaa ttaaaatgag taatatagat ccagaattaa
      421 ttgtaagaat taatgaattg gctaaaaaga aaaaagatgg tacaatcagt gaggctgaat
      481 tagaagaaca aaaagaatta cgtcaaaaat atttaaaagc ttttcgtagt gggtttaagc
      541 aacaattaat gggtgttaaa gttgttgacg aagaaggaaa tgatatcacg ccacaaaagc
      601 taaaagatga aaaattaaaa aattaatata atttgtcgaa aaacttattt taatttatac
      661 gattttgtgt ataataagaa taagttttta tgtgcaaaaa aggagaatta gaaattaatg
      721 gaaacatcaa gcttatcaat cgcaacaatt cgtgcgttag gtattgatac catcaacaaa
      781 gctaactctg gtcatccagg gatggtatta gggtcaacac cggcaatcta tacgttattt
      841 actaaggaaa tggattttta tcacaaacaa tcaaaatggt ttaatcgcga tcgttttgta
      901 ttggcttctg gtcatgcttc agcattgtta tattcaatgc tgcatttatc tggttatcaa
      961 atttctattg atgacttaaa gaattttaga caatggggtt ctattactcc aggacatcct
     1021 gaatgtgata ttactgatgg tgtcgatgct tcttcaggac ctttgggtca aggaattcca
     1081 atggcagcag gaatggcttt agcagaaaaa ttcttagctt caaaatataa ccgtgagggt
     1141 tatgatgttg tcgatcacta tacatatgca ttatgcggag atggtgatat gcaagaaggc
     1201 gttacttatg aggctgcttc tttagctggg catttatcgt taggtaaatt aattgtttta
     1261 tatgatgcca ataaagttac gcttgatggg ccgttatcaa tgtcttttag tgaagatgtg
     1321 aaaaagcgtt atgaagcaat cggatggcaa gtaattcgtg taacagatgg aaatgatgtt
     1381 agtgcaattc aaaaagcaat ccgtaaagga aaaaaagaat tatataaacc aactttgatc
     1441 attattgata cagtgattgg ttttggttca agtaatgaag gaactaataa agttcatggt
     1501 aatccattag gaaaagaaga tggtaaaaat gctaaaattt cttatggctt tgatcatgac
     1561 gaattttatg ttcctgaaga agtttatgat gattttgcta atacatgtat taaacgtgga
     1621 aaaaatcgtt ataataagtg gcagcggtta atgaaagact ataaaaatgc ttatccagaa
     1681 cttgctcaag aattagaaaa tgctattgct gggaactttt catt
//
LOCUS       DAOMTA010000718         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3581_length_1723_cov_2.130695, whole genome shotgun sequence.
ACCESSION   DAOMTA010000718 DAOMTA010000000
VERSION     DAOMTA010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3581_length_1723_cov_2.130695"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLK68_14865"
     CDS             <1..591
                     /locus_tag="PLK68_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91729.1"
                     /translation="EIICVKLRNSKNTETCILECYGYEHAKKTIENQIEVIKDDWHVV
                     IDSPIVEQIVSELYYESDQYKTGKAMDIFKNYCETKIKEFEPYLYTDEYGNSRKAEVL
                     LEYSYFRGYKDGLIVDAPEIIDSQDGIAYTAKNSNMIPIDCSMITFVELVKQMDFKYI
                     SDKCYENILEYWDEIFMTEILEMNNDEIQDENEVEL"
     gene            706..1716
                     /locus_tag="PLK68_14870"
     CDS             706..1716
                     /locus_tag="PLK68_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRM91730.1"
                     /translation="MKREMSKARQALVDSYIESLEKEIIPWKQGWSTVKNYNPVTKTQ
                     YRGINRLILYNAVAKNNYNDPRWMTFSQIKAANYKLNNAKGKGVPLEKCSLYDMETKK
                     YLNISDVDKIVREENLSRQEKYERFKWSVKVFYVFNASLIEGIEPYTIEEKDIHIDVN
                     DLACRFIDRYCKNANLSIVENFLCECPVYKPVNDMILIPAKGSFDDEYEFVSAVLHEC
                     CHSTMKENRLGRSYPNLKTNNERYAFEELYAEIASSFLCADIGIEIKQEKIDNHTAYV
                     QFWYKQIKKNPEIIFSALNGAEEISEYIEVKGELQELLIESKKEIYTEFDEETNTYDL
                     CV"
BASE COUNT          680 a          178 c          346 g          519 t
ORIGIN      
        1 gagattattt gtgttaaatt aagaaattca aagaacactg aaacatgtat tttagaatgt
       61 tatgggtatg aacatgcaaa aaaaacgatt gaaaatcaaa ttgaagttat taaagatgat
      121 tggcatgttg ttattgatag tcctatagta gagcaaattg ttagtgaatt atattatgag
      181 agtgatcaat ataaaactgg caaagcaatg gatattttta aaaattactg tgaaacaaaa
      241 ataaaggagt ttgaaccata tttgtataca gacgaatacg gtaattccag aaaagctgaa
      301 gtgttactgg agtattcata ttttagaggt tataaagatg gtttgattgt ggatgcgcca
      361 gaaataattg atagtcagga tggaattgct tatactgcaa agaatagtaa tatgattcca
      421 attgactgtt ctatgattac ttttgtagaa cttgtaaaac aaatggattt taaatacatt
      481 tcggataagt gctatgaaaa cattttagag tattgggatg agatatttat gacagaaatt
      541 ttggagatga ataatgatga aattcaagat gaaaatgaag tggaattata ggagaaagaa
      601 gatgaaaaaa gtacaaattt ttatatgcga tgaagagaca gaagtaagtg acgaagatgt
      661 aatagaagaa ttattttatc cactagattt tggagatgag taatcatgaa aagagagatg
      721 agcaaggcaa ggcaggcgtt agttgattct tatatagaat cactggaaaa agaaatcata
      781 ccatggaagc aggggtggtc tacagtcaaa aactataatc ctgttaccaa aacacaatac
      841 agaggaataa atcgccttat tctttataat gctgttgcaa agaataatta taacgatcca
      901 cgatggatga cgttttcaca aattaaagca gcaaattata aattaaataa tgcaaagggc
      961 aagggtgttc cattggaaaa atgttcttta tatgatatgg agacaaaaaa atacttaaat
     1021 attagcgatg tagataaaat tgtcagagaa gaaaatctca gtcggcagga aaagtatgaa
     1081 cgctttaagt ggtcggttaa agtattttat gtttttaatg cttcacttat tgaaggaata
     1141 gaaccctaca cgatagaaga aaaagacatt catattgatg taaatgattt ggcctgcaga
     1201 tttattgata gatattgcaa aaatgctaat cttagtattg ttgaaaattt tttatgtgag
     1261 tgccctgttt ataaaccagt aaatgacatg atcttaattc cagctaaagg tagttttgat
     1321 gatgaatacg agtttgtatc agcggtattg cacgagtgct gtcattcaac gatgaaagaa
     1381 aaccgattag gaagaagtta ccctaattta aaaacaaata atgaaagata tgcatttgaa
     1441 gaattatatg ctgagatagc ttcatctttt ttatgtgctg atattggaat agaaataaag
     1501 caggaaaaga tagataatca tactgcatat gtacaatttt ggtataagca gattaagaaa
     1561 aatcctgaaa ttatatttag cgctctaaac ggtgctgagg aaatttcaga atatatagaa
     1621 gtaaaaggtg aattacagga actgctgata gaatcaaaaa aagaaattta tacagaattt
     1681 gatgaagaaa ccaatacata tgatttgtgt gtttaaaaag agg
//
LOCUS       DAOMTA010000719         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3587_length_1721_cov_1.974190, whole genome shotgun sequence.
ACCESSION   DAOMTA010000719 DAOMTA010000000
VERSION     DAOMTA010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3587_length_1721_cov_1.974190"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLK68_14875"
     CDS             <1..445
                     /locus_tag="PLK68_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRM91731.1"
                     /translation="IQEKIEARRNKLKAIDGLSISSYKDKNGKEQFKATAQSEILFKF
                     DSYELNEEAQKMLSDLCNIITEIPNTKIKIIGHTDNIGEKQYNIILSKNRAAAVGNYL
                     RTAGIETNNITEDGKGYSEPIADNTSEAGRAKNRRVEIFITNDEY"
     gene            complement(748..1182)
                     /locus_tag="PLK68_14880"
     CDS             complement(748..1182)
                     /locus_tag="PLK68_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91732.1"
                     /translation="MADKLRKKQNEGISSVLEIAKMIRGEELHGSSINEAPIQEAKYE
                     VEEEKEPLHVEMETTSSEVFNFNGSSEMDLTDLLEYIKGKNYNCKEVLYVDTDVKEVF
                     GLLKAKAKIPISSLVSFILEDWLTKHRSDISSLIKQKKNRFL"
     gene            complement(1241..>1721)
                     /locus_tag="PLK68_14885"
     CDS             complement(1241..>1721)
                     /locus_tag="PLK68_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRM91733.1"
                     /translation="EDIATYDNEPKYEVIQTDISNVAKIISDFTKVEQGNLLIDLPGK
                     IDDDALGTILKAADIIICPFKYDKFTMDSTGFFIQVLQYLNVKAKIFFLPNNMRTAVR
                     YETKEQVVDILKQVGFVTDEIPASVAMERINTLAISNECIDKVKNAYDYIIQEGAIK"
BASE COUNT          630 a          312 c          235 g          544 t
ORIGIN      
        1 tatccaagaa aagatagaag ctagaagaaa taaactaaaa gctattgatg gcttatcaat
       61 ctccagctat aaagataaaa atggaaaaga acagtttaaa gcaacagctc aaagtgaaat
      121 cctattcaaa tttgattcat atgaattaaa tgaagaagct caaaaaatgc tttctgactt
      181 atgtaatatc attactgaaa tacccaatac taagattaaa ataattgggc atactgacaa
      241 tataggagaa aaacaataca atatcatact ttctaaaaat agagcagcag cagtaggaaa
      301 ttacctcaga accgctggta tagaaacaaa caacataaca gaagatggaa aaggatatag
      361 cgaacccatt gcagacaata caagtgaagc tggacgtgct aaaaatagga gagtagaaat
      421 atttatcaca aatgatgaat attaaaaaga gagatttcta ttaacaaaga ccgggaggta
      481 aaactctcgg tctttgtttt atagtctgga aagaaattga taccgtactt ttcggagttc
      541 cggcactgat gggcaaacat ggcatgatga aatgataccg aattatcatt cctggcacac
      601 cctgcagaac aaccttttga taccaaatac aaaaagcagc cggaagccgg acgcaaagaa
      661 ttgataccat aaaaccaaaa aagaaaagtg ttttctaaac tagcctaacc aaaatctttt
      721 gtttcacaaa acgacccttt ttctgtacta aagaaaacgg ttcttttttt gcttaatcaa
      781 agaacttatg tcgctacggt gtttagttaa ccaatcttcc aaaataaacg aaactaaaga
      841 tgaaataggt attttggctt tagctttgag caacccaaac acctctttta catctgtatc
      901 aacatataga acttccttgc aattataatt ttttccttta atgtactcta ataagtccgt
      961 caaatccatt tcactagagc cattaaaatt aaaaacttcc gaagaagttg tttccatttc
     1021 tacatgaagt ggttctttct cctcttcaac ttcatattta gcttcttgaa taggagcttc
     1081 atttatacta ctcccatgca attcttcacc tcttatcatt ttagctattt ccagaacact
     1141 agaaatacct tcattttgct ttttccttaa tttatcagcc atgtgcaaat atatacaata
     1201 aatgaataac tttataacaa atatctatta aaagaatatt ttattttatt gctccttctt
     1261 gaatgatata atcataagca ttcttcacct tatcaataca ctcattacta attgctaatg
     1321 tatttatacg ttccatagca acagaagctg gtatttcatc tgtaacaaaa ccaacttgct
     1381 taagaatatc aactacctgt tcttttgttt catatcttac cgcagttctc atattgttag
     1441 gtaaaaagaa tattttggct tttacattta aatactgtaa cacttggata aaaaaacctg
     1501 tactatccat tgtaaattta tcgtatttaa atgggcatat tattatatcc gcagctttaa
     1561 gaatagttcc cagagcatca tcatcaatct taccaggcaa atctattaat agatttcctt
     1621 gctcaacctt agtaaaatca ctaataattt tagcaacatt agatatatcg gtttgtataa
     1681 cctcatattt aggttcatta tcataggtcg caatatcttc t
//
LOCUS       DAOMTA010000720         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3589_length_1720_cov_2.743544, whole genome shotgun sequence.
ACCESSION   DAOMTA010000720 DAOMTA010000000
VERSION     DAOMTA010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3589_length_1720_cov_2.743544"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..797
                     /locus_tag="PLK68_14890"
     CDS             159..797
                     /locus_tag="PLK68_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM91734.1"
                     /translation="MIKDERYSGVLLYKGKKYTNIVPPIVTKSIQNKAIDSLKVGRSR
                     KNYRYIYDNIIYCANCNKVMTGTSGKGRNKIYYYYQCKECNGKISQEQINEVISTKKY
                     RMIKDGQKAEIKEIDKKRYSLNRRIKNLRERYLYKKITEREFCSLIIPLEDELDCLNL
                     KRKVLIKMDNDIDFLSLSVREKKSYIHSRVRKITVDTVNKTVKNIVYKDIGN"
     gene            893..1069
                     /locus_tag="PLK68_14895"
     CDS             893..1069
                     /locus_tag="PLK68_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRM91735.1"
                     /translation="MSVAGNKKRVFITLDLNILEQLEFLAEKKGVNKSSLISFWISDE
                     YERVKNSEKKDEKK"
     gene            complement(1487..1562)
                     /locus_tag="PLK68_14900"
     tRNA            complement(1487..1562)
                     /locus_tag="PLK68_14900"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1527..1529),aa:His,seq:gtg)
BASE COUNT          741 a          181 c          286 g          512 t
ORIGIN      
        1 cagggacgaa gataaaaaca ttcaacttga taaaaaagta tataatataa tcagaaacgc
       61 tttcaactac gtgattgaag aatgggaaac atcagatgtt agagattatt taaataagtc
      121 tcaaaaaaaa acagtttagt acagaatctg ttaaacagat gattaaagat gaacgatatt
      181 cgggtgttct cttgtataaa ggaaagaaat atacaaacat agtgccacca atagttacaa
      241 aaagtataca aaataaagct atagacagtt taaaggtagg acgttcgaga aagaattatc
      301 gatacatata cgataatatt atatactgtg caaattgtaa taaagtaatg acaggaacaa
      361 gcggtaaagg cagaaataaa atatattatt attaccagtg taaagaatgt aatggcaaaa
      421 tttctcagga gcaaattaat gaagtaatca gtacaaaaaa atataggatg attaaagacg
      481 gtcaaaaagc tgagattaaa gagattgata aaaaaagata ttcattaaac agaaggataa
      541 aaaatttaag agaaagatac ctgtataaaa agattactga acgtgagttc tgttcattaa
      601 taataccttt agaggatgag ttggattgtc ttaacctgaa acgcaaagtg ttaataaaaa
      661 tggataatga tatagatttt ttaagtcttt ctgttagaga aaagaaatca tatattcaca
      721 gtcgagttag aaagattaca gttgatacag tgaacaaaac agtaaaaaat atagtgtata
      781 aagatatagg aaattaacaa aacacgagaa atcgtgtttt gttcatatat atgttgcaaa
      841 agtgtaacat atatatgata taattatatt gtaataaaaa gaggtgacgg aaatgagtgt
      901 tgcaggaaat aaaaaaagag tatttattac attggatcta aatattttgg aacagcttga
      961 atttctggct gaaaaaaaag gagttaataa aagctctttg atatctttct ggatcagtga
     1021 tgaatatgaa cgagtaaaaa attctgaaaa aaaagacgaa aaaaaataaa caatttgtat
     1081 tattcttatt atgattgtga tagaataatg gcaaagaaaa aaacaaaaca aaataaaaaa
     1141 gaatacctta tttgtccacc taggcattct taatacgata atttccagtc gaattggaaa
     1201 ctaaatgttg atttcaaata gaaatcagat tagtatgtta tttttataac attatatata
     1261 ttaataaata aattacttaa tttatttgct aatattataa catattaatt tgtttaatca
     1321 atagtcgaat attaagataa gccaaatgga aaggtatccg tttggttttt gttttactat
     1381 agtttgcaaa agtttacaaa gtgcagtttt actttatttg tcaaagtgcg ttgtgcgttt
     1441 ttactatatt ttgcaaagtg cgaaatgttt cgactaaaaa tataaatggg gcggtcgatg
     1501 ggaatcgaac ccacgaatgc cagagccaca atctggtgtg ttaaccactt caccacgacc
     1561 gccgtattta ctttgttaag aggttattaa ctctcaacgc aagaatgatt atatataaaa
     1621 atttgataaa tgtaaagaga aaatgtaaaa aaaaataaaa aattgaaaaa atttattcag
     1681 ttgagcataa aaaaaaaaaa acatatttaa acaaaattac
//
LOCUS       DAOMTA010000721         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3599_length_1719_cov_1.928486, whole genome shotgun sequence.
ACCESSION   DAOMTA010000721 DAOMTA010000000
VERSION     DAOMTA010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3599_length_1719_cov_1.928486"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1589
                     /locus_tag="PLK68_14905"
     CDS             369..1589
                     /locus_tag="PLK68_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM91736.1"
                     /translation="MKKLGKAILGLALALSMCFSMFIPVYAEGETVDTLVGSLIGYYR
                     DGARTDVLRTLDTLKEVSKDDYDQWQSIIDYWDWIENDMVENIGVAPDGLPNDNTHAF
                     VVLGFALKSDGTMQPELVGRLETALASAQKYPNSYILVTGGVKKNGWTEGDRMHDWLV
                     EHGVADNRILVENKSANTAQNAEFSFDILYNHPTIKSISMITSQYHLKRGTILYYAES
                     LIRARELGKTPIKLNGNAGWYREDKTSETLSQKASSLSQIAGVSIPYGEKLPKSKLTG
                     IEVTGKNTYTVGDALDIIVTSTYDTEYSRDVTGLAKIIGFDANKVGKQNVVVVYTETL
                     HHSTSSSEDVTMTAEFEVEVKTAPITVPVNPQPTPDKPVNAVNTGDNSPLAIMTMVAG
                     LAAAGMFLAKRKEN"
BASE COUNT          614 a          223 c          328 g          554 t
ORIGIN      
        1 aaagctatat taattatgct gttcataatt taaaagcact gatgtcttct atgtaggatt
       61 attggatacc ggaattgccc aaaaaatgta ttgaaacacc tagtatagtg atgaaataga
      121 ggtaacttct atccttaatc aaaaaatatt ctttacaatt gacttatata aataaaaggg
      181 aatgataatg attatatttc attatcattc ccttttagta tttttggcta cttttatatt
      241 ttaacatatt cggcaaacaa aaacattaaa tatttaatgt tttctttttg tgcaaaaaat
      301 attttttgta tagacaaaag tggtatgata ttaataaaga agtcataaca taaggaggat
      361 aaaaagttat gaagaagtta gggaaagcga tacttggctt agctttagca ctgtcgatgt
      421 gctttagtat gttcattcca gtgtatgctg aaggggaaac agtagataca ctagttggca
      481 gtttaatcgg ttattatcga gatggtgcta gaacagacgt tttgagaact ttggatactt
      541 taaaagaggt ttctaaagat gattatgatc aatggcaatc aatcattgat tattgggact
      601 ggattgaaaa cgatatggtc gaaaatattg gtgtagctcc tgatggtcta ccaaatgata
      661 atacccatgc ttttgttgta ctaggttttg cattaaagag tgatggaaca atgcagccag
      721 aattagtagg acgattagag actgctttgg ctagtgctca aaaatatcct aattcatata
      781 ttttagtaac tggtggtgta aagaaaaacg gttggactga aggggaccgg atgcacgact
      841 ggttagttga acatggtgta gcagataatc gaattctagt tgaaaataaa tcagctaata
      901 cagcacagaa tgctgaattt agttttgata ttctttataa tcatccaaca attaagagta
      961 tttctatgat tactagccaa tatcatttaa aacgtggaac aatcttatac tatgcggaat
     1021 cattaattcg agcacgtgag ttagggaaga cgccaattaa attaaatggt aatgcaggat
     1081 ggtaccggga agataaaact agtgaaacat tatcacaaaa agctagcagt ttatcacaaa
     1141 ttgctggagt ttctattcct tatggtgaaa aattaccaaa atctaaatta acaggaattg
     1201 aagttactgg taaaaatact tatactgttg gagatgcatt agatatcata gttacttcaa
     1261 cttatgatac tgaatatagt cgtgatgtta caggtcttgc taaaattatt ggatttgatg
     1321 ctaataaagt cggtaaacaa aatgtagttg tagtttatac ggaaacttta catcattcta
     1381 caagtagttc tgaagatgta actatgacag ctgaatttga agttgaggta aaaacagctc
     1441 caattacagt tccagtaaac ccacagccaa ccccagataa acctgtaaat gcagtaaata
     1501 ctggagataa cagcccattg gcaataatga cgatggtagc agggttagca gctgctggaa
     1561 tgtttttagc aaaaagaaaa gaaaattaat agctatagtg aaagctgaat cgcaaaatga
     1621 ttcagctttt tttagtatat caatgataat ggaattaagt tttatttatt gaataatatt
     1681 tgtttgaccg taatcttaaa atattgtaag attatatta
//
LOCUS       DAOMTA010000722         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3601_length_1719_cov_1.810697, whole genome shotgun sequence.
ACCESSION   DAOMTA010000722 DAOMTA010000000
VERSION     DAOMTA010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3601_length_1719_cov_1.810697"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLK68_14910"
     CDS             complement(<1..225)
                     /locus_tag="PLK68_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HRM91737.1"
                     /translation="MIKTYQVEKDNYQDAGKASSDIKRTLKALGIDRKILKAVAIACY
                     EAEINIAIHSDGGTVTFEIDDEGLIQLLFDD"
     gene            complement(222..575)
                     /locus_tag="PLK68_14915"
     CDS             complement(222..575)
                     /locus_tag="PLK68_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91738.1"
                     /translation="MLVKEIVDILKAKEIYIDDQAIYQKDYKKGFSSDLMSDALALLK
                     NETEEVLFITGLANVQSLRTAEVLDIDTILFVRGKPLDMTIVDMAKNLHINLFQTDET
                     MFEACGKLYEAGMRR"
     gene            complement(569..>889)
                     /locus_tag="PLK68_14920"
     CDS             complement(569..>889)
                     /locus_tag="PLK68_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRM91739.1"
                     /translation="KVYGVVTFYSQFTTEPKGKHVIAVCLGTACYVNGSQTILDLLCE
                     MTGCEVNSTSPDGLFSIDATRCVGACGLAPVVSVDGIVFGCTKQLEDLKMLVLDYLKE
                     EAPC"
     assembly_gap    890..899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1269..>1719
                     /locus_tag="PLK68_14925"
     CDS             1269..>1719
                     /locus_tag="PLK68_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM91740.1"
                     /translation="MNLLHLKYVIEVAKAGSISKAASNLYMNQPHLSKTIKDLEENMQ
                     IIIFERTSKGVIPTKKGAEFIERAKSIIMQVEEMEAMYQGYDDKSVHLDICVPRASYV
                     ANAFTNYLSMINFHDKNIKVNYRETNSFDTIKDVYEGEANIGIIRFPI"
BASE COUNT          562 a          286 c          267 g          594 t
ORIGIN      
        1 atcatcaaat aagagctgaa tcaatccctc atcatcaatt tcaaaagtaa ctgttcctcc
       61 atcggaatga attgcaatat tgatctcagc ttcatagcaa gcaatcgcta ctgcttttag
      121 aatttttcgg tcaattccta gggcttttag agtccttttg atatcacttg atgctttccc
      181 ggcatcttga taattatctt tttctacttg ataagtttta atcatcgtcg cattcctgcc
      241 tcgtatagct tgccgcaagc ttcaaacatt gtttcatcag tttggaagag attaatatga
      301 agatttttag ccatatcaac aattgtcatg tctagtggct ttcctctaac aaataaaatt
      361 gtatcaatat ctaaaacttc tgctgtcctt aaagattgta cgttggctag accagtaata
      421 aataatactt cttcggtttc gtttttcaat aatgctagag catctgacat taaatcagaa
      481 gagaagccct ttttatagtc tttttgatag attgcttgat catcaatata tatttccttc
      541 gcttttagta tatcgacaat ctctttaact aacatggggc ttcttctttc aaataatcta
      601 agacaagcat ttttagatct tctaattgtt tagtacatcc aaaaacaatt ccgtcaacac
      661 tgacaactgg cgcaagacca caggcgccaa cgcagcgtgt tgcatcaatt gaaaatagtc
      721 catcaggact agtagaattt acttcgcatc ccgtcatttc acaaagtaag tctagaatag
      781 tttgggaacc attgacataa caagctgttc ctaaacatac ggcgataaca tgtttacctt
      841 taggttcagt tgtaaattga gaatagaaag tgactacacc gtatactttn nnnnnnnnnt
      901 agatactgga acaccaattg tttcagaaat tttttccatt gcttcaaaag gaatatagcc
      961 taattcgttt tgaatctcgt gaagcataag tttcattgga cctttttcat ctttgtgctt
     1021 agcaacgatt tcgtcaattt tatccaaata ttcttgtttc aactttttca tttttacacc
     1081 tctttctttt ttatcatttg atttaattat aacataagtt gaaagcgctt taaatacaat
     1141 taagtcaaaa gtaccatata tttgtaagtt atagcgatag ataaaaaaat atatatttga
     1201 taaatatgct agaatataaa cacaatttat cttttctatg ttaaaataat cactaaggag
     1261 atattgggat gaatctatta catttaaaat acgtgattga agttgcaaaa gccggctcaa
     1321 ttagtaaagc tgccagcaat ttatatatga atcagccgca tttaagtaaa acaattaaag
     1381 atctggaaga aaatatgcag ataattattt ttgaacgaac ttcaaaagga gttattccaa
     1441 caaaaaaagg tgctgaattt attgaacgag ctaaaagtat cattatgcag gtagaagaga
     1501 tggaagctat gtatcaagga tatgatgata agtctgttca tttagatata tgtgtaccgc
     1561 gggctagtta tgtcgctaat gcgtttacta attatttatc gatgattaat tttcatgata
     1621 aaaatattaa ggttaattat cgcgaaacga attcttttga tacaatcaag gatgtttatg
     1681 aaggagaagc aaatattgga ataattcgct ttcctattg
//
LOCUS       DAOMTA010000723         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3610_length_1716_cov_2.391331, whole genome shotgun sequence.
ACCESSION   DAOMTA010000723 DAOMTA010000000
VERSION     DAOMTA010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3610_length_1716_cov_2.391331"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="PLK68_14930"
     CDS             complement(<1..1708)
                     /locus_tag="PLK68_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRM91741.1"
                     /translation="MAWYKGEFEISNKNATKDPLDLRVADDSSQTTIVVNDQKTYQSV
                     LGMGTSIEESTIYNLLKMTDENRQAFLRRLLDPVNGMGMSLMRVTVGTSDFTAQDFYT
                     YYDGTGKELDGKPDWNNVTGKGFSIQKDKDLGIIKVINEMQAIAKELGVEDNLKFFAS
                     SWTPPGWMKLPTSSSNSYEDNELLLKGGKLNDDYIDDLAKYMVRYVEEYQKCGIPIYA
                     MTIQNEPLLEINYPSCAMTGTQEAKIAKAIKAELAKSTVLKDNEKEVKLWAFDHNFDG
                     ADKFMADFFKEAGDQLNIDGIAFHPYGGNASTMGSFYDRYKDQLSMNLTERSVWGTSG
                     ANDIITWLRNGSESYNSWVTMLDSNVGTHHWVGTPDPTLFVQDANNPQRYWATPEVYI
                     MSQFTKYVKPGYVRVDTNNGSSSTVTNVAFKDPETGKIVMIVANRSGTDQKFKVMMKG
                     AQFNAVLPAGNVATYIWDGSDIEINALDVPGTFTADKCSSATNANINTTDHIIDYVNK
                     NASIDYLVDVKEAGTYNVMIKVASGEDWENKTVNVKSGDTVVGTTVAQRFNFWGGEWQ
                     DYR"
BASE COUNT          538 a          360 c          205 g          613 t
ORIGIN      
        1 aacggtaatc ttgccattca ccaccccaga aattaaatct ttgtgccaca gtagtaccaa
       61 caactgtatc acctgattta acgtttactg ttttattttc ccaatcttca ccagaagcaa
      121 ctttaatcat aacattatat gttccggctt cttttacatc aaccaaataa tctatactcg
      181 catttttatt tacataatca ataatatggt ctgttgtatt aatattagca ttagtagctg
      241 aactacattt atcagctgtg aatgttcctg gaacatctaa tgcatttatt tcaatatcac
      301 taccatccca aatatatgtt gcaacattac cagccggtaa taccgcatta aattgagcac
      361 ccttcatcat tactttgaat ttttgatcag taccacttcg gtttgcaaca atcataacaa
      421 tttttcctgt ttcaggatct ttaaacgcaa cgtttgttac agttgaacta gaaccattat
      481 ttgtatcaac acggacatat cccggtttta catatttagt aaattgcgac atgatatata
      541 cttctggagt agcccaatat ctttgaggat tattagcatc ctgcacaaat aacgttggat
      601 ctggagttcc aacccaatga tgagttccaa cattactatc taacatcgta acccagctat
      661 tataactttc agaaccgttt cttaaccaag taataatatc attagcacca ctagtacccc
      721 atactgatct ttccgttaag ttcatactta attgatcttt atatctatca tagaaagaac
      781 ccattgttga agcattacca ccataaggat ggaatgcaat accatcaata tttaattgat
      841 caccagcttc tttaaagaaa tcagccataa atttatcagc accatcaaaa ttatgatcaa
      901 acgcccatag tttaacttct ttttcattat cctttaacac agtgcttttc gctaattcag
      961 ctttgattgc tttagcaatt tttgcttctt gggttcctgt cattgcacaa ctaggataat
     1021 taatttctaa taatggctca ttctgaatcg tcattgcata gataggaata ccacattttt
     1081 gatattcttc aacataacga accatatatt tagctaaatc atcaatataa tcatcattta
     1141 atttaccacc cttcaataat aattcattat cctcatatga attactactt gaagtaggta
     1201 acttcatcca ccctggtggt gtccaagatg aagcaaagaa tttcagatta tcttctactc
     1261 ctaattcttt tgcgatagcc tgcatctcat taataacttt aataattcct aaatctttat
     1321 ctttttgaat actaaatcct ttaccagtaa cattattcca atctggttta ccatctaatt
     1381 cttttcctgt accatcataa taagtgtaga agtcttgtgc cgtaaaatct gatgttccca
     1441 ctgttacccg cattaacgac attcccatac cattaacagg atctaataat cttcttaaga
     1501 aagcttgacg attttcatca gtcatcttta ataaattgta aattgttgat tcttcaattg
     1561 aagttcccat tcctaaaaca ctttgatatg ttttttggtc atttacaaca attgttgttt
     1621 gactagaatc atctgcaaca cgtaaatcaa gaggatcttt tgttgcattt ttattagaaa
     1681 tctcaaattc acctttatac caagccatat cttgtg
//
LOCUS       DAOMTA010000724         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3613_length_1716_cov_2.051776, whole genome shotgun sequence.
ACCESSION   DAOMTA010000724 DAOMTA010000000
VERSION     DAOMTA010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3613_length_1716_cov_2.051776"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLK68_14935"
     CDS             <1..441
                     /locus_tag="PLK68_14935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM91742.1"
                     /translation="HILKNNLSYACILIDIDYFKKINDTYGHIIGDRFLIEIVKIIKC
                     FFRASDLIGRIGGDEFLIFIKDIYDKEIVCEKANALIKKIHEYVTANNYPKEISISMG
                     IHIDNRPEISFTELYNKVDIALYNAKHNGRDRYVYYHEGMTYPK"
     gene            complement(582..>1716)
                     /locus_tag="PLK68_14940"
     CDS             complement(582..>1716)
                     /locus_tag="PLK68_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91743.1"
                     /translation="FATLSKGQQTKVLLAILFCKPHDFILIDEPTNHLDYHGRLLLSK
                     YLKRKKSFILVSHDRDFLDRCIDHVLAINRNSIEVIKGNFSLWYDLKMNNDKREIEKN
                     EQLKKDIGRLKKAARQNEDWSNKVEATKSVKVAGLKPDKGYVGHKAAKMMQRSKNIER
                     RQSKAIEEKSDLLKNIETIEKLKIFPLEYSKQQLCYFNHVTISYDKHIIVENLSFDIN
                     QGDRLNLAGKNGSGKTSIIKIIMKESNNYQGKVNIGNNLKIAYLSQDDSHLKGKLDDY
                     ANSLDIDLSLFKAILRKLDFSRELFTQEISTYSKGQKKKVMLAGVLCQEAHLFILDEP
                     LNYLDIFTRMQVQELLLIFKPTVLFVEHDKYFCDQIKTKTLNL"
BASE COUNT          574 a          283 c          203 g          656 t
ORIGIN      
        1 catatcttaa aaaataatct ttcctatgct tgtatcttaa ttgacattga ttattttaaa
       61 aaaatcaatg atacttatgg tcatatcatc ggtgatcgtt ttctaattga aattgtaaaa
      121 ataattaagt gtttctttcg agcaagtgat ttaattggaa gaattggtgg tgatgaattt
      181 ttaatcttta tcaaagatat atatgataaa gagatcgtct gcgaaaaggc aaatgcttta
      241 attaaaaaaa ttcatgaata tgtcactgcc aataactatc ctaaagaaat tagtatttct
      301 atgggaattc atatcgataa tcgaccagag atatctttta ctgaattata taataaagtt
      361 gatattgctc tatataatgc taaacacaat ggacgagatc gttatgtcta ttaccatgaa
      421 ggtatgactt atcctaaata attgttaaag accatataaa atgaagcgcc cctaaaagct
      481 cgacatagcc aactaaaagg ggttgcttca agatttacat agtctttaat tatcattatt
      541 acttttgttt tcttctcata ttctaactca cttgaccttc tttataagtt aagcgtttta
      601 gttttaattt gatcacaaaa atatttatca tgttcaacaa ataatactgt cggtttaaat
      661 attagcaata attcctgaac ctgcattcgc gtaaaaatat ctaaataatt taatggttca
      721 tctaaaataa ataaatgagc ttcttggcat aacactcccg ctaacattac ttttttcttt
      781 tgacctttac tataagtact aatctcctga gtaaatagtt cgcgagaaaa atctaatttt
      841 cttaaaatag ctttaaacaa acttaaatca atatcaagac tatttgcata atcatccagc
      901 tttcctttta aatgactatc atcttgactt agatacgcta tttttagatt atttccaata
      961 tttacctttc cttgataatt attactttct ttcataataa ttttaattat acttgtcttc
     1021 cctgaaccat tttttccagc aagattaagt cgatctccct gattaatatc aaaacttaaa
     1081 ttttcaacaa taatatgttt atcataacta atcgtaacat gattaaaata acatagttgc
     1141 tgttttgagt attcaagtgg aaagatcttt aatttttcga ttgtttcaat attttttaat
     1201 aaatcacttt tttcttcgat tgctttactt tgacgtcgtt caatattttt agagcgttgc
     1261 atcattttag ctgctttatg accaacataa cctttatctg gttttaaacc agcaaccttt
     1321 acacttttag tagcttccac tttatttgac caatcttcat tttgacgagc tgcctttttc
     1381 aaacgcccaa tatctttttt taactgctca ttcttttcta tttccctctt atcattattc
     1441 attttcaaat cataccaaag ggaaaaattc ccttttatca cttcgatact attacgattt
     1501 atagctaaga catgatctat acaacgatct aaaaaatcac gatcatggct taccaatata
     1561 aaacttttct ttcgtttcaa atatttactt agtaaaagtc gaccgtgata atccaaatga
     1621 tttgttggtt catcaattaa aataaaatca tgaggcttgc aaaaaagaat ggccaataat
     1681 actttagttt gttgtccttt tgataaggta gcgaaa
//
LOCUS       DAOMTA010000725         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3632_length_1706_cov_2.062386, whole genome shotgun sequence.
ACCESSION   DAOMTA010000725 DAOMTA010000000
VERSION     DAOMTA010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_1706_cov_2.062386"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLK68_14945"
     CDS             <1..294
                     /locus_tag="PLK68_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRM91744.1"
                     /translation="TENLDISFDNTLCKCPKCGGNLYINKKAYNCSNYRNEAIKCDFV
                     IWREMSGRSITPEEAIELCEKKETPVLTGFHDKNGQPMERKLVLNDDFKIKLI"
     gene            339..644
                     /locus_tag="PLK68_14950"
     CDS             339..644
                     /locus_tag="PLK68_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91745.1"
                     /translation="MKVKGDWNFPHLPRIGEEISPHIIMFQNEFTYQNLLEYLTDEAK
                     SDFNKFNDGEDDLEGNFKAWVYDVICEVNIVESIHYRPDTEDYTQIIPEICLSDLSN"
     gene            662..934
                     /locus_tag="PLK68_14955"
     CDS             662..934
                     /locus_tag="PLK68_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM91746.1"
                     /translation="MNKTDLIKMVAEKANKPTKEVAEIIDSFFKELSQNLREKDVSIK
                     DFGTFKKIIQPARIARNPATGEPVEVPEKEVVKFKPSKNILNMKWL"
     gene            931..1197
                     /locus_tag="PLK68_14960"
     CDS             931..1197
                     /locus_tag="PLK68_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91747.1"
                     /translation="MIEINIHIPIDGVNNISIPIQLPVVPRVGEKIYLSDKQINNLIS
                     LLEEEYYHNPQTEEWKESIRRFTNIEEVAYNAANGTIHIELSTI"
     gene            1208..1651
                     /locus_tag="PLK68_14965"
     CDS             1208..1651
                     /locus_tag="PLK68_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91748.1"
                     /translation="MKKYLSLVMIGLSLMLSGCNKDDNNDPNTPPTGDTGKVRYEATV
                     SDPENFKLLIHYTVGVDISSEAPEEAAKEIIVESPFTFEQEAKQGTYLWLSAYPIPKD
                     EENLENLQLPKTVEVKIFIDDKLHKSNSGENYAMVQHIFGQENYQ"
BASE COUNT          680 a          244 c          303 g          479 t
ORIGIN      
        1 acagagaact tagatatttc ttttgacaat acattgtgta agtgtcctaa gtgtggagga
       61 aacttatata taaacaaaaa agcctataat tgttccaact atagaaatga agccataaaa
      121 tgtgattttg tcatttggcg tgaaatgtca ggacgttcta taactcccga agaagcaata
      181 gaactttgcg agaaaaaaga aacacctgtg ttaactggat ttcatgataa aaacgggcaa
      241 ccgatggaaa gaaagcttgt actgaacgat gattttaaaa taaaactaat atgaaaatag
      301 aatttataat ttatagccat ttttttaaag agagaggaat gaaggtaaag ggtgattgga
      361 attttccgca tcttccaagg ataggcgaag aaatttcacc tcacattata atgtttcaga
      421 atgaatttac ctatcaaaat ttattagaat atctaacaga tgaagctaaa agtgatttta
      481 ataaattcaa tgatggtgag gatgatttag aaggaaattt taaagcatgg gtatatgatg
      541 ttatctgtga ggtcaatata gttgaatcaa tacattatag accagataca gaagactata
      601 cccaaattat tcctgaaatt tgtttaagtg atttaagtaa ttaaatatta ttgatatgca
      661 aatgaataag actgatttaa tcaaaatggt tgctgaaaag gcaaataaac caacaaaaga
      721 agtagcagaa ataattgata gttttttcaa agaattatct caaaatctaa gagaaaaaga
      781 tgtttctata aaagattttg gtactttcaa aaagattatt cagccagctc ggatagctag
      841 aaatccggca actggagaac ctgtagaagt tccagaaaag gaagttgtta aatttaagcc
      901 gtccaaaaat atactaaaca tgaaatggct atgatagaaa taaacatcca tattcctata
      961 gatggtgtga acaatatttc tataccaatc caattacctg tagttccaag ggttggagaa
     1021 aagatttatc tatctgacaa acaaataaat aatttgataa gtctattaga agaagaatat
     1081 taccacaatc cacaaacaga agaatggaaa gaatcaataa gacggttcac caatattgag
     1141 gaagtagcat ataatgctgc aaatggaacg atacacattg aattatcaac tatttaaaag
     1201 aaacaatatg aaaaagtatt tatcattagt aatgattggt ttaagcctta tgttgtctgg
     1261 atgcaataaa gacgataaca acgaccctaa tactccacct acaggagata caggaaaagt
     1321 acgttatgaa gctaccgtaa gcgacccaga aaatttcaaa cttctaatac attatacagt
     1381 aggagtagat atttcttccg aagctcccga agaagctgcc aaagaaatta ttgtagaaag
     1441 tccattcaca tttgagcaag aagccaaaca aggaacatat ttatggctat ctgcctaccc
     1501 tatacctaaa gatgaagaaa accttgaaaa tttacagcta cctaagacag tcgaagtaaa
     1561 aatttttatt gatgataagc tgcataagtc taacagtggt gaaaattatg caatggttca
     1621 acatatattc ggacaagaaa attaccagta gaattgatac cttttctgcc tgcactggag
     1681 agaaaccggg aaggtatttg ataccg
//
LOCUS       DAOMTA010000726         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3633_length_1706_cov_2.014537, whole genome shotgun sequence.
ACCESSION   DAOMTA010000726 DAOMTA010000000
VERSION     DAOMTA010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_1706_cov_2.014537"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PLK68_14970"
     CDS             complement(<1..1648)
                     /locus_tag="PLK68_14970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91749.1"
                     /translation="MINKLKGIFNNPLKKELDFIQQNTFDIKNTELYNRWLEENCSFV
                     PARKYSYNPKISIVMPVYNVPGEYLGYCIDSILNQTYQNFEICIADDCSTNQDTINTL
                     KKYMQKDKRIKVVFRKDNGQIPQKPTNSALELVSGEFVGLMDNDDKLEIHALNEVISV
                     LNDNPNLDFIYTDEDKIDMQEQRSDPHFKSDFAMHSLYGGNYICHFSVIRKKLIDKIG
                     GFRIGYEGAQDFDLFLRLIDNTNKIYHIPKILYHWRMIPGSTAVGGDGAKNYAGEAGK
                     RALEDYFKNKNVKVNIDIVISTQYFVEYIIDKEPKTTVIFVAKTSAENAINAIDRICK
                     DNVYTNFDIIVINDTKLDLMYLESNIVKIVNVEENAILAINNTVNKLTSEYIIFTDEN
                     NYYETIDWMYNLIGYASRPETGVIGAKIIGKYDLVRKSGIVLNNKSILLNAFSPTYRD
                     DYGNNGRLLVPYNYTVVANELFALKRDDFISLESSFNYEFSCYDLQLKLDEQDKQNIF
                     IPQVAVKNKFVNSDFEKKQLLLLKEKWKNKLNYDKLYNENL"
BASE COUNT          567 a          281 c          171 g          687 t
ORIGIN      
        1 ataaattctc attatagagt ttatcataat ttaatttatt cttccatttt tcttttaaca
       61 gtaatagctg ttttttttca aaatcactgt ttacaaattt attttttaca gcaacttgtg
      121 gaataaatat attctgttta tcttgttcat ctaattttaa ttgtaaatca taacatgaaa
      181 attcataatt aaatgatgat tctaaagata taaaatcatc cctttttaga gcaaaaagtt
      241 catttgccac aacagtatag ttataaggta ctaaaagtct accattatta ccataatcat
      301 ctctatatgt tggtgaaaag gcatttaata aaatactctt attatttaaa acaattcctg
      361 attttcttac caaatcatat ttacctatga tttttgcacc tattacacca gtttctggtc
      421 ttgaggcata tcctattaaa ttatacatcc aatctattgt ttcataataa ttgttctcat
      481 ctgtaaaaat gatatattct gaagtcaatt tattaacagt attattaatt gctagaatag
      541 cattttcttc tacattaaca atcttaacaa tgttgctttc taaatacatt aaatctagct
      601 ttgtatcatt aattacaata atatcaaaat ttgtgtatac attatcttta caaattctat
      661 ctattgcatt aattgcattc tctgcacttg ttttcgcaac aaatataaca gttgttttcg
      721 gttctttatc tataatatat tcaacaaaat attgcgtact aatgactatg tctatattta
      781 cttttacatt tttgttttta aaataatctt ctaaagccct ttttccagct tcacctgcat
      841 aatttttggc tccatctccg cccaccgctg tagaaccagg aatcattctc caatgatata
      901 aaattttagg aatatgatat atcttattag tattatcaat caatcttaga aataaatcaa
      961 aatcttgtgc accttcatat ccaattctaa atccgcctat tttatcaatt aattttttcc
     1021 ttataacact gaaatggcaa atataattac caccatataa tgaatgcata gcaaaatcag
     1081 atttaaaatg cggatcactt cgctgttctt gcatatcaat tttatcttca tctgtataaa
     1141 taaaatctaa attaggatta tcatttaata ccgatataac ttcatttagc gcatgtattt
     1201 ctaatttatc atcattatcc atcaatccaa caaattcccc agaaaccaac tctagtgccg
     1261 aattagttgg tttctgggga atttgtccgt tatcttttct aaatacaact ttaattcgct
     1321 tatctttttg catgtatttt tttaacgtgt taattgtatc ttgatttgtg gaacaatcat
     1381 cagcgataca aatttcaaaa ttttgatatg tttgatttaa aatcgaatcg atacaatatc
     1441 ccaaatattc tccaggtaca ttgtaaaccg gcataactat agaaatttta ggattataac
     1501 tatatttacg agctggaaca aagctacagt tttcttcaag ccatcgattg tataattcag
     1561 tattttttat atcaaaagta ttttgttgta taaaatctag ttcctttttc aaaggattat
     1621 taaatattcc tttaagttta tttatcatta taccacgcgg tctatattta tttataattc
     1681 gctttactac atttgattta actaca
//
LOCUS       DAOMTA010000727         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3635_length_1705_cov_2.931515, whole genome shotgun sequence.
ACCESSION   DAOMTA010000727 DAOMTA010000000
VERSION     DAOMTA010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_1705_cov_2.931515"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLK68_14975"
     CDS             complement(<1..867)
                     /locus_tag="PLK68_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91750.1"
                     /translation="MYKLIKLILYRIKHNKAFLITYLVLIPIIIALAIYFTNSISHTI
                     QIGIVGDIDTSQNSQIKYTYLDTLPSTSELVLNQYDAIMVQDDKNIDVLSTKGETYNQ
                     TITLLVNGQIDSLAVNDNQRGSASNILGFLMMVILLLGGQIYKYYFDERTGINKRILS
                     TSVSCYQYLLSHFIVVYIFLFIPATIVICSALFVFKITLSIALWKFILILALLCFFAT
                     SFGLWSNVLSKSLEESMMFGNMFAIIGTIVSGGITQVTNNEIFNYVVQFFPQKQVMTT
                     LSALENKNVLPTS"
     gene            complement(879..1625)
                     /locus_tag="PLK68_14980"
     CDS             complement(879..1625)
                     /locus_tag="PLK68_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91751.1"
                     /translation="MIKFWTLFKHDLYNLISSPGTLSVFLIFPTILILLMGFLFDNLY
                     HTTIISSYDFYGVTMIFFIAMMGATVPANAFLEKHIKNGNTRIFYSPVSRVSIYSSKI
                     LTCFLFMALALTINIIIFDTISLVNFGSDKIGYVILLIINFVLFLTILSSAICVTLHS
                     EELTNVILSNSMSVLGFLSGIFFPIASLGAIFEKIASFSPIKWTVDCIFQLIYDGQST
                     NYWWIMFALLTLSSLLLLVVHKNYRPEDYI"
BASE COUNT          631 a          253 c          288 g          533 t
ORIGIN      
        1 tgaagtcggt aaaacgtttt tgttttctag agctgaaagc gtagtcataa cttgtttttg
       61 agggaaaaat tgaacaacat aattaaaaat ttcattattt gttacttgag tgatcccgcc
      121 agagacaata gttccaataa ttgcaaacat atttccaaac atcatacttt cttccaaaga
      181 ttttgataag acattactcc aaagaccgaa tgaagttgca aagaaacata aaagtgctaa
      241 gattaaaatg aatttccaga gtgcaatcga taaagtgatc ttaaaaacaa aaagagcact
      301 gcaaatgact atcgtggcag gaataaaaag aaaaatataa acaacaataa aatgactcaa
      361 taaatactga taacaactta ctgaagtact caagatgcgt ttgttgatgc cagttcgttc
      421 atcaaagtaa tatttataaa tttgtccgcc aagaagaagt atcaccatca ttaagaagcc
      481 taatatattt gaagcactgc cacgctgatt atcattaacg gctaagctgt ctatttgacc
      541 atttactaag agggtgatag tttggttata cgtttcacct ttagtggata agacatcaat
      601 atttttatca tcttggacca ttatggcatc atattgattt aagacaagtt cggaggtact
      661 aggaagagta tctagataag tatatttgat ctgagaattt tgagatgtgt ctatatctcc
      721 cacgattcct atttgtattg tatgagaaat gctgttggta aagtatattg caagtgcaat
      781 gataatagga atcaaaacaa gataagtaat tagaaatgct ttgttgtgct tgattcgata
      841 taatattagt tttatgagtt tgtacattct ttttcctcct aaatatagtc ttctggacga
      901 tagtttttgt ggacaacaag tagtaaaagt gaagatagtg tcaaaagagc aaacatgatc
      961 caccagtaat tagtactttg tccatcgtaa attaattgaa aaatacaatc gacagtccat
     1021 ttgattggtg aaaaacttgc aatcttttca aaaatagcac ctaaactcgc gattgggaaa
     1081 aagattccag atagaaagcc taatacagac attgagtttg ataaaataac atttgtgagt
     1141 tcttcactat gaagagtaac acaaatagca ctgcttaata ttgtaaggaa tagaacaaag
     1201 tttattatta agagaattac atatccgatt ttatcactac cgaagtttac caatgatatc
     1261 gtatcaaaaa taatgatatt gattgttaag gcaagtgcca tgaataaaaa acatgtgaga
     1321 atctttgagc tgtagatcga cacgcgagat acagggctgt aaaagattcg ggtatttccg
     1381 tttttgatat gcttttctag aaaagcatta gcagggacag tagcgcccat catcgcaata
     1441 aaaaatatca ttgttacacc gtaaaaatca taggaactga ttatagttgt atgatataaa
     1501 ttatcaaata aaaaccccat taataaaatt aagattgtag gaaaaataag aaaaacagat
     1561 aaggtacctg ggctggaaat aagattatat agatcgtgtt taaataaagt ccaaaatttt
     1621 atcatgatca atcccttagc ttcttcccgg ttagacctaa aaataccatt tctaaatttc
     1681 cttcttctgt ttccatttta ttaat
//
LOCUS       DAOMTA010000728         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3646_length_1703_cov_2.379248, whole genome shotgun sequence.
ACCESSION   DAOMTA010000728 DAOMTA010000000
VERSION     DAOMTA010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3646_length_1703_cov_2.379248"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>648
                     /locus_tag="PLK68_14985"
     CDS             24..>648
                     /locus_tag="PLK68_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91752.1"
                     /translation="MKQLLEFFASYNFTIDENDPDDAEIGVDPEMLGRIFENLLEDNK
                     DKGAFYTPKEIVQYMCRQSLIAYLQTDISDEDTKESINSFVKTYDVKSLGGENSKLAI
                     MLDQKLKEVKICDPAIGSGAFPMGLLKELFLCRGAIEKFDDAASIKRHIMQQNIYGVD
                     IEKGAVDIARLRFWLSLVVDEYSPQSLPNLDYKIMQGNSLLEQYNGVD"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <659..>1703
                     /locus_tag="PLK68_14990"
     CDS             <659..>1703
                     /locus_tag="PLK68_14990"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRM91753.1"
                     /translation="AVRKQEIINNNMSLDIFDDIMDVYRNDLRKKITAYYSCCNHEEK
                     RQLLTDIHENILKQIDEQRLPVDLHDLDIQANDKFFLWHTWFSDVFNRPNGCNGFDIV
                     IGNPPYFLYQESHLGEINNIRKDKDYNIAFGGKLNAYKLFIANALNKLLSKNGVNCFI
                     FQNSFLGDRQATKLRYNVLSENKILNIDSYPERDSKKKRVFESVKMSVCIMLVQKSKV
                     SSNYNFPVNIWDDKETTTGIHTIFSFDDICSIDKVDYTIPRIRQKDKALVVKLIKKKE
                     IAIKCIEGELNMTFHKKYFTNKESYPLILKGAAIQRYFYTKQMSQGKIEYLDEERYLK
                     DCGNTEKSKHHNLD"
BASE COUNT          637 a          238 c          296 g          522 t
ORIGIN      
        1 aatttccatt accttctgaa tttatgaagc aactactgga attctttgca tcatataatt
       61 tcacaataga tgaaaacgat cctgatgatg cagaaatagg agttgatcca gagatgctag
      121 gtcgtatttt tgaaaaccta ttggaagaca ataaagacaa aggtgcattc tacactccaa
      181 aagaaattgt acaatacatg tgcagacaat ctctaattgc atatttacaa acagatatta
      241 gtgacgaaga tactaaggaa tctataaatt catttgtaaa gacttacgat gtgaagtcac
      301 ttggcggaga aaatagcaaa ttggcaataa tgctagatca aaaattgaag gaagtgaaaa
      361 tctgtgatcc tgctattggt tcaggagcct tcccaatggg tctactaaaa gagttatttc
      421 tatgtcgcgg agcaatcgaa aaatttgatg atgctgcatc gataaaacgg catattatgc
      481 aacaaaatat ttatggagtt gatattgaaa agggagctgt tgatattgct cgcctccgct
      541 tttggctttc tttggtcgtt gatgaatatt cacctcaatc cttacctaat cttgactata
      601 agattatgca agggaattca ttattagagc agtataatgg cgttgattnn nnnnnnnngg
      661 cagtacgcaa gcaagaaata attaataata atatgtctct tgacatattt gatgatatta
      721 tggatgttta ccgaaatgat ttacgtaaaa aaattactgc atattattct tgttgcaacc
      781 atgaagaaaa acgccagctt ctgacagaca ttcacgaaaa tatattaaag caaatagatg
      841 agcagagatt acctgttgat ttacatgatt tagacattca agccaatgac aaattctttc
      901 tatggcacac atggttttct gatgtattca atcgaccgaa tggttgcaac ggatttgata
      961 ttgttatcgg gaaccctcca tattttcttt atcaagaatc tcacttagga gaaattaata
     1021 atattagaaa agataaagat tataatatag cttttggagg aaaattaaat gcatataaat
     1081 tattcattgc aaatgcttta aacaaattgt tatctaaaaa tggcgtaaat tgtttcatat
     1141 ttcaaaattc ttttttagga gatagacaag caaccaaatt aagatacaat gttttgagcg
     1201 aaaataaaat tttgaatatt gattcatatc cagaacgcga tagtaaaaaa aagagagtgt
     1261 tcgagagtgt taaaatgagt gtgtgtatta tgcttgttca aaaatctaaa gtatcatcta
     1321 attacaactt ccctgttaat atatgggatg ataaagaaac aacaactggg atacacacta
     1381 tcttttcttt tgatgacatt tgttctatag acaaggtaga ttatactatc ccaagaatac
     1441 gccaaaaaga caaggctttg gtggtgaaat taataaaaaa gaaagaaata gctattaaat
     1501 gcatagaagg agaattaaat atgacatttc ataaaaaata ttttacaaat aaagagtcgt
     1561 atccgttaat tctaaaagga gcagctattc aacgttattt ttatacaaaa caaatgagcc
     1621 aaggaaaaat tgaatactta gatgaggaaa gatatttgaa agattgtggc aacactgaaa
     1681 aatcgaaaca tcataatctt gat
//
LOCUS       DAOMTA010000729         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3648_length_1703_cov_2.260922, whole genome shotgun sequence.
ACCESSION   DAOMTA010000729 DAOMTA010000000
VERSION     DAOMTA010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3648_length_1703_cov_2.260922"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLK68_14995"
     CDS             complement(<1..1319)
                     /locus_tag="PLK68_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRM91754.1"
                     /translation="MIKDDFLTLTAQKFINTYFTSRNFDQVKNMFAPEGSWLGASNNT
                     MANNLKQFDQYFKEGCNEGYVPEITFQDCYVIYNDDNIGIVYCNYHLHIESKGALFDM
                     DQRVTIVLKQFNTTLKIIHVHASTPNDAISQEEFYTNEIAKLGYKELQQALKKKNEQI
                     EMIIRTTAGGMKGSLDDEFYTYFYVNEELCKMLGYTYDEFMEMSHGTAVGAVYPPDLP
                     AALADCKRCFAIGPEYKTEYRIRKKDGSLLWVMDSGRKVTDENGQVVINSIITDISEL
                     KEMVNRLKIDQERYEIVSQLSDDIIFEYDVENNSLVYQQLQADTPVKNILTNFLTVDL
                     KNTHLYHGDIERFKKDLKIILSNQVSNELYKLEYRFAIAPQSYTWYRLTFRRIFDNDN
                     KLTKVVGKVVDISSELRLKHQSITDPLTGTYNRLYITSAIQEYCHIL"
BASE COUNT          535 a          273 c          240 g          655 t
ORIGIN      
        1 tttaagatat gacaatattc ttgaatcgcg ctagtaatat ataaacggtt atatgttccg
       61 gttaggggat ctgttattga ctggtgtttt aatcgtaatt ctgaggaaat atcaacaact
      121 ttgccgacaa ctttagttaa tttattatca ttatcaaata tccgtcgaaa agttagacgg
      181 taccaagtat aactttgtgg tgcgattgca aaacgatatt ccaatttata taattcatta
      241 gaaacttgat ttgataaaat tatcttcaaa tcctttttaa agcgttcgat atcaccatga
      301 tataaatgag tattttttaa atcaactgtc aaaaaattag tcaatatatt tttgactgga
      361 gtatcagctt gtagctgttg gtatactagt gaattatttt caacatcata ttcaaaaata
      421 atatcatcag ataactgaga tacaatttca tagcgttctt gatcaatttt taaacgattg
      481 accatttctt taagttcaga gatatcagta atgatactat ttattactac ttgaccgttt
      541 tcatctgtaa ctttacgtcc gctatccatt acccataata aacttccgtc tttttttcga
      601 atccgatatt ctgttttgta ttctggtccg atggcaaaac atcgtttaca atctgctaaa
      661 gcagcaggta aatcaggtgg atagactgcg ccaacagcag taccatgact catttccatg
      721 aattcatcat aagtataacc aagcatttta cagagctctt cattaacata aaagtaagta
      781 taaaattcat catcaagact acctttcatt cccccggcgg ttgttctgat aatcatttca
      841 atttgttcat tttttttctt taaagcttgt tgtaactctt tatatccgag tttagcaatt
      901 tcattggtat agaattcttc ttgactaatt gcatcattgg gggtcgaggc atgaacatga
      961 ataattttaa gtgtagtatt aaattgtttt aaaacaatcg ttacccgttg atccatatca
     1021 aaaagggctc ctttgctttc gatatgtaaa tgataattac aataaacgat tccaatatta
     1081 tcgtcgttgt agataacata acaatcttga aaggtaattt caggaacata accctcgtta
     1141 cacccctctt taaagtattg atcaaattgt ttaaggttgt ttgccatagt attattacta
     1201 gcccctagcc atgagccttc tggagcaaac atattcttga cttgatcaaa atttctgcta
     1261 gtgaaatatg tattgataaa tttttgagca gttagagtta aaaagtcgtc tttgatcatt
     1321 gtttgcaccc ccttacgttt agtttaatag taacacttat tattagtaat gttaataaga
     1381 agatttcaaa taaaaaaaga attgtggttt actcagccac aattcttttg attttcgaaa
     1441 cgttttcaac aactttttaa tttcactttt catctacgca tcttctatta ctttgcctta
     1501 tattatcatg ttgttttaac gtttctttaa aaggctcctg ttctaatttt ttattattat
     1561 gtttttttag aactcttcct tttatctact tcaaatatat acctaaaaag atgatttttc
     1621 aagcatcttt caaatgttgt tgtttttatt tatttttata taatttatag tataattaaa
     1681 tttaaggggt gaaaaaagta ttg
//
LOCUS       DAOMTA010000730         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3649_length_1703_cov_1.753034, whole genome shotgun sequence.
ACCESSION   DAOMTA010000730 DAOMTA010000000
VERSION     DAOMTA010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_1703_cov_1.753034"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLK68_15000"
     CDS             complement(<1..993)
                     /locus_tag="PLK68_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91755.1"
                     /translation="MEKKRIDILNGSIWDKILAFALPLAATSVLQQLFNSADVAVVGH
                     FSGSNALAAVGSTSPITNLFITIFVGLSIGANVVISRFLGAQNEKDTSKAVHTSILLS
                     IISGIVIALIGEVIAVWLLKIMSTPKEVLDQAALFLRITFIGMIFLTIYNFEAAILRA
                     GGDTKRPLYCLLISGVVNVTLGLFFVVVCKLDVAGVALATLIADATSALLLFYILTKE
                     QGPLKLSLEKLKIDKTITKDILFTGIPAAIQGMLFNVSNIIIQSGINSLGADVVAAST
                     VGLNFEIYVYYLITGFSQASITFNSQNYGAGNYKRCIKSTRGCMILGTIFTVSLS"
     gene            complement(1241..>1703)
                     /locus_tag="PLK68_15005"
     CDS             complement(1241..>1703)
                     /locus_tag="PLK68_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HRM91756.1"
                     /translation="SKELKNYQIYLIPDKPFSKLIAVIIPTFIKIFVVAAVSFIAMGL
                     YYHQSLMMIIVYLLNVIGYTSIFISGSVLSVRLLKSRTSPMMENFMRMIVMLIGAIPS
                     AVITTAILLNSGTTVAMMAASYVALFVNFAISFLILYGCRNMMNGRELKSE"
BASE COUNT          631 a          322 c          207 g          543 t
ORIGIN      
        1 acttaatgaa actgtaaaaa tagtccctaa gatcatacag cctcgagtag atttgataca
       61 tcttttatag ttgcctgctc cataattttg actattgaat gtaatactag cttgtgaaaa
      121 tccagttatt aaatagtata cataaatttc aaaattcaaa ccaactgttg aagccgcaac
      181 aacatcagct cctaaactgt ttattcctga ttgaataata atattagaaa cattaaataa
      241 catcccttga atagctgcag gaatcccagt aaataagata tctttagtaa tagtcttatc
      301 tatttttagt ttttctagcg ataatttcaa tggtccctgt tctttagtta agatataaaa
      361 taataataaa gcactagttg catctgcaat cagtgttgct aatgctactc ctgcaacatc
      421 taatttacaa acaacaacga aaaatagtcc taaagtaaca tttacaacac cagaaatcaa
      481 taagcagtat agtggtcgtt ttgtatctcc accggccctt agtattgctg cttcaaaatt
      541 ataaatcgtt aaaaaaatca ttccaataaa cgtaattcgt aaaaacaaag cagcttgatc
      601 gagtacttct tttggtgttg acataatttt caatagccag actgcaataa cttcaccaat
      661 taaagcaatg acaatcccac taataatcga aagtaatatt gaagtatgca ctgctttact
      721 agtatctttt tcattttgag cccctaaaaa ccgcgaaata acaacatttg ctccgattga
      781 taaaccaaca aatattgtaa taaataaatt agtaatcggg ctggtactcc caaccgctgc
      841 caaagcatta cttcctgaaa aatgtcctac aacagcaaca tctgcagaat taaataactg
      901 ctgcaacaca cttgttgctg ccaacggtaa tgcaaatgcc agtattttat cccaaatact
      961 tccgttcaaa atatcaattc tttttttttc cataataaat cccccaattt tctcacttat
     1021 tataaatctt ctactatact ttaaggcaag ggattttcaa aaactgaaaa gaagcttatt
     1081 ttccaagttt tactttatct aaaaataaaa cgtagcactt attcaactac gttttatgaa
     1141 tacttcatta tatacaattt taataacagt aaatagttaa tatatctgta atatcaaaat
     1201 taaaaaggta ttgatcaaaa ctaatcaata cccatattat ttattcactt tttaattctc
     1261 gtccattcat catattacga catccatata aaatcaaaaa cgatattgca aagtttacaa
     1321 atagtgcaac atagctagct gccatcatcg ccacggtagt gccgctattc aacaagatgg
     1381 cagtagtgat tacagcactt ggaatcgcac caatcaacat cactatcatt cgcataaaat
     1441 tctccatcat tggtgaagtt cgactcttta aaagtctaac agatagtaca cttccactaa
     1501 taaagatact agtataacca ataacattta atagatagac aatgatcatc attagacttt
     1561 gatgataata taggcccatt gcaataaaag aaacagctgc aacaacaaat atctttataa
     1621 atgttggaat gattaccgca atcaatttac taaacggctt atcaggaatt aaataaattt
     1681 gataattttt taattcttta gat
//
LOCUS       DAOMTA010000731         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3656_length_1701_cov_2.958080, whole genome shotgun sequence.
ACCESSION   DAOMTA010000731 DAOMTA010000000
VERSION     DAOMTA010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3656_length_1701_cov_2.958080"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLK68_15010"
     CDS             complement(<1..1258)
                     /locus_tag="PLK68_15010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM91757.1"
                     /translation="MTFPKDFLWGGAVTAHQSEGAYNAGGKSPAVCDLFPKPEHSDFK
                     DGIDSYHRYDDDFSLFEEMGFTSYRFSIDWSRVCPDGINFSEESMQFYDDFIDSMIRH
                     GMEPMCSLYHFEMPQLLMNKYNGFYSREVVDMFVTYANKMIDRYGDRVKKWISFNEQN
                     AIALPGSSKVAYGAVCPKDIDEQTFINQLVHNTFVAHAKVVEKVHTIADAKVLGMVIY
                     IPAYAATCNPLDELESRNQMALTDMYFDMFTYGEYSSYMMAKMKNENNLPKMLDGDLE
                     LLKKNKVDWLSLSYYFSTVASHGKLSIEMNGNAKAATNPYLKASEWGWQIDPLGLRIG
                     LRDIYAKYRLPIMVVENGFGMRDILENETVIDDTRIDYMKDHLEQIALAINEGVDCRG
                     YLMWGPIDILSSQGEMSKRYGTIYVNR"
     gene            complement(1458..1643)
                     /gene="rpmB"
                     /locus_tag="PLK68_15015"
     CDS             complement(1458..1643)
                     /gene="rpmB"
                     /locus_tag="PLK68_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRM91758.1"
                     /translation="MSRKCQISGKGPMSGNTRSHALNSSRRKWNVNLQKATILVDGKP
                     TTVRISARELRTLRKSA"
BASE COUNT          549 a          328 c          247 g          577 t
ORIGIN      
        1 cccgattaac ataaatagta ccatagcgtt tagacatctc tccttgagaa ctcaaaatgt
       61 caattggtcc ccacattaaa tatccacgac agtcaacacc ttcattaatt gctaatgcga
      121 tttgctcaag atgatctttc atgtaatcaa ttcttgtatc atcgataacg gtttcatttt
      181 ctaaaatatc acgcattcca aagccgtttt caacgaccat aatcggtaaa cgatatttcg
      241 cataaatatc ccttaaacca atacgtaaac caaggggatc gatttgccaa ccccattcac
      301 tagcttttaa atatggattg gtagctgctt ttgcattacc gttcatttca atgcttagtt
      361 tgccatgtga agctactgtt gaaaaataat aacttaatga tagccagtca accttattct
      421 ttttgagcaa ctctaaatct ccatctagca tttttggcag attattttca tttttcattt
      481 ttgccatcat gtaacttgaa tactccccgt acgtaaacat atcaaaatac atgtctgtaa
      541 gtgccatttg atttcgagat tctagctcgt caagaggatt acaagtggcg gcataagcag
      601 gaatatatat taccattcct aagactttag catcagcgat agtatgaact ttttcaacaa
      661 ctttagcatg agcaacaaaa gtattgtgaa ccagctgatt aataaatgtt tgttcgtcaa
      721 tgtcttttgg acaaacggca ccataagcta ccttactgct gcctggtaaa gcaattgcat
      781 tttgttcgtt aaatgaaatc cattttttta cacgatcacc ataacggtca atcattttat
      841 tagcataggt aacaaacata tctactactt cccgtgaata aaatccgtta tatttattca
      901 ttagcaattg gggcatttcg aagtgatata atgagcacat tggctccatt ccatgccgaa
      961 tcattgaatc aataaaatca tcatagaatt gcatactttc ttcactaaag ttaataccat
     1021 ctgggcatac tcttgaccag tcaattgaga aacgataact ggtgaagccc atttcttcaa
     1081 ataatgaaaa atcgtcatca taacgatgat agctatcaat tccgtcttta aaatcactat
     1141 gttctggttt agggaagaga tcacacactg caggactttt accacctgcg ttataagcac
     1201 cttcgctctg gtgagcggtt acagcaccac cccatagaaa atcttttgga aatgtcattt
     1261 aattatcctc ctaatttata tagtattatc atattaggaa ataattgagg tgtaaataca
     1321 ttggccttgt ttattaatat agtttcattg aataagaaaa taatgtaaat ttaatgaaaa
     1381 aaagtttcaa aaaaaccaat catagaataa ctataattgg ataaaataaa aatagtaccg
     1441 cagtactatt tatattatta agctgatttt ctcaaagttc ttaattctct agcagaaatt
     1501 cttacagtcg taggtttacc atctactaaa atagtagctt tttgtaaatt aacattccat
     1561 tttcttctac ttgagtttaa tgcatgagaa cgtgtatttc cagacatagg tcctttacca
     1621 ctgatttgac attttcttga cataggttct cctcctcttc attagtagtg ttttaacgta
     1681 cttgcatata gtatcatttt t
//
LOCUS       DAOMTA010000732         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3667_length_1698_cov_2.668290, whole genome shotgun sequence.
ACCESSION   DAOMTA010000732 DAOMTA010000000
VERSION     DAOMTA010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_1698_cov_2.668290"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /gene="lspA"
                     /locus_tag="PLK68_15020"
     CDS             complement(<1..368)
                     /gene="lspA"
                     /locus_tag="PLK68_15020"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRM91759.1"
                     /translation="MNLTKKNKILYLVTLILVVGGDLFTKHLVSSSMLLGQSHEIINN
                     FFYFTYAHNTGVAWGMFAGKLGLFIVVAIIAAVVMIVFFRKTKSEEVLTRFGLVLTFG
                     GMIGNLVDRIFLGYVRDFIDV"
     gene            complement(800..1168)
                     /locus_tag="PLK68_15025"
     CDS             complement(800..1168)
                     /locus_tag="PLK68_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HRM91760.1"
                     /translation="MVNCRIESMYLCVNDMDRAVSFYEQFFEQRVTKKDEIYSIFDIN
                     GFRLGLFAYKKMNEEHIFGSNCLPSIEFLNKEVLKAKISSYKLCFPLTPIGTNWVVEI
                     IDSEGNHIELTAPIEIVKES"
     gene            complement(1308..>1698)
                     /locus_tag="PLK68_15030"
     CDS             complement(1308..>1698)
                     /locus_tag="PLK68_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRM91761.1"
                     /translation="LIAGTYTPICLSYLEYPHSIYFLAVIWAIACVGIIIKLFWMNAP
                     RFISTIFYLLMGWALIFDLPAFSKVPLGCLGLIACGGISYTIGAIVYIVKKPNWFKTF
                     GFHELFHIFVMIGSAFHFLAVIIYILL"
BASE COUNT          639 a          321 c          170 g          568 t
ORIGIN      
        1 acatcgataa aatctcggac atatcctaaa aagattcgat caactaagtt tccaatcatt
       61 cctccaaaag tcaacactag accaaaacgt gtcaacactt cctcactttt tgtctttcta
      121 aaaaatacga tcatcacaac tgctgcaata atagcaacaa caataaataa ccctaattta
      181 ccagcaaaca tcccccaggc aactccagtg ttatgagcat aggtaaaata aaagaaatta
      241 tttatgattt catgagactg tcctaataac attgatgatg atactaaatg ttttgtaaac
      301 aagtcaccac caactactaa tatcaatgta actaaatata atattttatt tttttttgtt
      361 aaattcatta tttttatcac ccaccgacat tatacaataa tctaaaacta accacaagaa
      421 aaagcaccac tgctcattta gtaaaaaatt aataaacttc tcagcttaaa attactgttt
      481 cattgatgat agcagtttta gcaatattaa cgactttgat taagttttta aaacgtgtat
      541 tcctgaaact taataggtca cttactactc ttgataatga ttaatgtgat tggtgccaaa
      601 gctacttgta aatcttcttt tatatattta tcattgacat ttcttttcat tattaacatt
      661 ttagtaattt ctaaagtttg aattatattt ttaataatgt tacttgagct gtttcaatcc
      721 taaattcaga ttgcatctta ctacaattat caatactggt ttcaattact tttaacgctt
      781 caatgaaata attattcatc tagctttctt ttactatttc tattggcgca gtcaattcaa
      841 tatgattacc ttcagaatca attatttcta caacccaatt agtacctatt ggagttaatg
      901 gaaaacataa tttataagag ctgatttttg cttttaaaac ttctttatta agaaactcaa
      961 tactaggtaa acaattactg ccaaatatat gttcttcatt cattttttta taagcaaata
     1021 aaccaagacg gaaaccatta atatcaaaaa tactatatat ttcatctttt tttgtcactc
     1081 tttgttcaaa aaactgttca taaaaactaa ctgccctatc catatcattg acacataaat
     1141 acattgattc aatcctacaa ttcaccattt catttctcct ccctttaaaa taatgatcat
     1201 tattttaaac ttttactgct cctaatcccc ttgttatatc actctaattc taccactgta
     1261 aatactagat gtcactttat aaaaaaagaa caaattaaat ttgtccttta caataaaata
     1321 taaatgatga ctgccaaaaa atgaaaagca gagccaatca ttacaaatat atggaacaat
     1381 tcgtgaaaac caaatgtttt aaaccaattt ggcttcttca caatatagac gattgcccca
     1441 attgtataac taatgccacc acaggcaatt aatcccaagc aaccaagcgg aaccttacta
     1501 aatgcgggta aatcaaaaat cagtgcccat cccattaata aataaaaaat agtagaaata
     1561 aatctcggtg cattcatcca aaataattta atgataatac caacacaagc aattgcccat
     1621 attactgcca aaaaataaat actatgcgga tactctaaat atgacaaaca aataggagta
     1681 tacgtaccag caatcagt
//
LOCUS       DAOMTA010000733         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3668_length_1697_cov_2.945189, whole genome shotgun sequence.
ACCESSION   DAOMTA010000733 DAOMTA010000000
VERSION     DAOMTA010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_1697_cov_2.945189"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1108)
                     /locus_tag="PLK68_15035"
     CDS             complement(173..1108)
                     /locus_tag="PLK68_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HRM91762.1"
                     /translation="MNDIFDKRLARPMLIANQSEPFNSPEYLFELKFDGIRCLAYIDR
                     KSVDLRNKRNKELINQFPELSNIFSNVNGQCILDGEIIVLKNGKPDFYEVQKRTVITD
                     FFKIKLLSEKYPATFVVYDLIYLDNDLLIDIPIEERINKLPRLINEDNRISISKVIEN
                     TGIELFNIVKSKELEGVVAKRKGSKYFFEKRSTDWIKFKYLVDDDFVLCGYIKKDNNM
                     TSFILGKYDSGNNLKYHGHVTLGASIRTLNQYNYTIIKHHPFNCDLPKGNDNAVWIEP
                     NLVCTVQYMPSNKEGMRQPVCKGIREDKETKECRE"
     gene            complement(1111..>1697)
                     /locus_tag="PLK68_15040"
     CDS             complement(1111..>1697)
                     /locus_tag="PLK68_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRM91763.1"
                     /translation="EKGKYVTISNNELERIKSKTDKTIHVLHFAKMTDINQLYYDKNY
                     YIVPEPGGEKAFELLRQALLSQKKVAIAKTVMGTKEELIVLYPTKTDIIAKILFFQEE
                     IQPAPTINKVSVDKNELEMAKTLINSITNKFAPEAYHDEYQAKLHDAIQTKINGQEVV
                     IADDSQPNSVIDLMSALQQSIDLANTQASNNMAS"
BASE COUNT          526 a          270 c          252 g          649 t
ORIGIN      
        1 atctaaaacg gaaaagacga tctgtttggc tgtttctcct tctcttaaac agtattacta
       61 cagtgagaat agagataaaa taatagaaaa caattcaata aatcaaagtt gttgataaac
      121 tcaatagttt taatcctcta attttatgat tgtctaaacc aagtaagaac cattattctc
      181 tgcactcttt tgtttctttg tcttctctaa tacctttaca aacaggttga cgcatacctt
      241 ctttgttaga aggcatatat tgtactgtac atactaaatt aggttctatc caaactgcat
      301 tatcatttcc tttgggtaga tcacaattaa atggatggtg ctttatgata gtgtaattgt
      361 attgatttaa tgttcttata cttgcaccca aagtgacatg tccatggtat tttaaattat
      421 tacctgaatc atatttacct aatataaaac ttgtcatatt gttgtctttt ttaatatatc
      481 cgcagagaac gaaatcatca tcaaccagat atttaaattt aatccagtca gttgaacgct
      541 tttcaaagaa atatttacta ccttttcgtt tagcaactac tccttccagt tcttttgatt
      601 tgacaatatt aaataactct ataccagtat tttctataac ttttgatata gatatacgat
      661 tatcttcatt aattaatcgt ggcaatttgt ttattctttc ttctattggt atatcgataa
      721 gtaagtcatt gtcaagatat attaaatcat agacaacaaa ggtagcagga tacttttcag
      781 ataagagttt tattttaaaa aagtcagtta taactgtacg cttttgaact tcgtaaaagt
      841 caggtttacc gtttttaagg acaatgatct ctccatcaag aatgcattgt ccattaacat
      901 tggaaaaaat attagaaagt tcaggaaact gattaatcag ttctttgtta cgcttatttc
      961 ttaaatcaac actttttcta tcgatatatg caagacatct gattccatca aactttaatt
     1021 caaataaata ctctggtgag ttgaacggct cagattgatt agcaattagc attggtcttg
     1081 ctaaacgttt gtcaaaaata tcattcatag ttacgatgcc atgttatttg atgcttgtgt
     1141 gttagctaga tcaatagact gctgcagtgc agacattaaa tcaattacac tgttaggttg
     1201 actgtcgtca gcaattacta cttcctgacc gtttatttta gtctgtatag catcatgtaa
     1261 tttggcttga tattcatcgt gatacgcttc tggtgcaaat ttatttgtta tagagttaat
     1321 taaggtttta gccatttcaa gttcattttt atcaacagaa accttgttaa tagttggagc
     1381 tggttgaatt tcttcctgaa aaaatagtat tttagcaatg atgtctgttt tagtgggata
     1441 cagcactata agttcctcct ttgtccccat aacggtttta gcaatggcaa ctttcttttg
     1501 agataatagt gcttgtctga gtagttcaaa tgccttttca ccaccaggct ctggaacaat
     1561 gtaataattt ttatcataat ataattgatt aatgtcggtc attttagcaa aatgtaatac
     1621 atgaatggtt ttatcagttt ttgatttgat tctctcaagt tcattgttag atattgtaac
     1681 atatttacct ttttcat
//
LOCUS       DAOMTA010000734         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3670_length_1697_cov_1.993910, whole genome shotgun sequence.
ACCESSION   DAOMTA010000734 DAOMTA010000000
VERSION     DAOMTA010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3670_length_1697_cov_1.993910"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLK68_15045"
     CDS             complement(<1..243)
                     /locus_tag="PLK68_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91764.1"
                     /translation="MSTILGSCNKKTANPNKNITIDAFSKMKMQGYSVRSYHIRGQID
                     SLCKYDKDSTVTDYRVRSYYLNRKPFLWIDRNGIDKR"
     gene            complement(282..479)
                     /locus_tag="PLK68_15050"
     CDS             complement(282..479)
                     /locus_tag="PLK68_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91765.1"
                     /translation="MNISVTGSNVHITGANGQTLFVYNVAGVCVASFRIEGADKHYEL
                     NLQRGCYILKIGNTVRKISLR"
     gene            complement(695..1609)
                     /locus_tag="PLK68_15055"
     CDS             complement(695..1609)
                     /locus_tag="PLK68_15055"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRM91766.1"
                     /translation="MEPFKVHVLGCGSALPTLRHSASSQIVEIREKMFMIDCGEGTQM
                     QLRRSRIRFTKISAIFISHLHGDHCFGLIGLISTFGLLGRTSPLHIYAPSEYKKVLDI
                     ELDMFCKQLGYELIFHPVNTTLNQVIYEDKSLTVSSIPLDHRMPCCGFIFVEKPTSRH
                     IIGDMMKIYEVPISQRNNIKNGADWTTPDGEVIKNEFLTKPADSVRSYAYCSDTKYLP
                     NLHTYIKNVNLLYHESTYSDEFKILAAKYYHSTARQAASVARDANAGKLMLGHYSSRY
                     ESEQVLLDEAKEVFSNTILSEEMKTIDV"
BASE COUNT          571 a          317 c          224 g          585 t
ORIGIN      
        1 cctcttgtct ataccattac ggtcaatcca caaaaatggt tttctattaa gataataact
       61 ccgtacacga taatcagtta cagttgaatc tttatcatat ttgcataaac tgtcaatctg
      121 tccacgtata tgatatgaac gtacagagta cccttgcatt ttcattttag aaaacgcatc
      181 aattgttatg ttcttattag ggttcgctgt ttttttatta caactaccca atatcgttga
      241 caaaactatt atcaatatac aatataaatt acgtctcaaa actatcttag cgaaattttg
      301 cgtaccgtat ttcctatttt taaaatatag caacctcttt gtaaatttaa ttcataatgt
      361 ttatcagccc cttctattcg aaatgaagca acacatactc cagctacatt atatacaaat
      421 aacgtctgtc cattagcacc agtaatatgg acatttgaac ctgttactga gatattcaca
      481 tcttgaaagt catttatctc aacatatctg tacggctcag caacgcttat ttctggtacg
      541 caaaatgaag ttattacagt taatattaaa agtaatatat ttttattcat acataatttt
      601 atattaaatt gcaaatttaa aattttgttt tgaattaacg aaactttttt actaaaaagc
      661 acaataatcg cataaaaaca aatactaaaa atgactaaac atcaatagtc ttcatctctt
      721 ccgaaaggat tgtgtttgag aaaacttctt ttgcctcatc caacaatact tgttcacttt
      781 catatcttga cgaataatgt ccaagcatca atttacccgc atttgcatct ctagctacag
      841 aagcagcctg acgagctgta ctatgataat attttgcggc cagtatttta aattcgtccg
      901 aatatgtgga ttcatgatat aacagattga cattttttat atatgtgtgt aagttcggta
      961 aatattttgt atccgaacaa tacgcatagc ttcttacact atctgcaggc ttagtcaaaa
     1021 attcattctt tatcacttca ccatctggtg tagtccaatc agcaccattt tttatattat
     1081 ttcgctgact gataggcact tcatatattt tcatcatatc tccaataata tgacgagaag
     1141 ttggtttttc gacaaatata aatccacaac aaggcattct atgatcaagt ggtattgatg
     1201 atacagtaag acttttatct tcatatatga cctgatttaa agttgtattt acaggatgaa
     1261 atatcaattc atatcctagt tgtttacaaa acatatccaa ctcaatatcc aacaccttct
     1321 tatattcaga tggagcatat atatgtaaag gagaagtgcg tcctaacaga ccaaacgtag
     1381 aaattaaacc aataagtcca aagcaatgat caccgtgtaa atgcgatata aatatagcac
     1441 ttattttagt aaagcgaata cgagaacgtc ttaattgcat ctgagttccc tcaccacaat
     1501 caatcataaa cattttctca cgtatctcca ctatttgaga tgatgcagaa tgtctaagag
     1561 tcggtagtgc gcttccacaa cctaaaacat gtactttaaa tggttccact taatatttat
     1621 tgcttcttga atattcgaat ttatctgcag aattttgtat gcatagagaa tcagctccca
     1681 gtattttaat gctaaaa
//
LOCUS       DAOMTA010000735         1695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3677_length_1695_cov_2.968293, whole genome shotgun sequence.
ACCESSION   DAOMTA010000735 DAOMTA010000000
VERSION     DAOMTA010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_1695_cov_2.968293"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /gene="rpsH"
                     /locus_tag="PLK68_15060"
     CDS             <1..246
                     /gene="rpsH"
                     /locus_tag="PLK68_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRM91767.1"
                     /translation="VEGEGPIKNINITLKYRGNDRVITDLKRISKPGLRVYAKVNEIP
                     KVLNGLGIVILSTSQGLMTDKEARAKQVGGEVLAYIW"
     gene            277..822
                     /gene="rplF"
                     /locus_tag="PLK68_15065"
     CDS             277..822
                     /gene="rplF"
                     /locus_tag="PLK68_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRM91768.1"
                     /translation="MSRVGNKIINVPEGVTVDIAADNTVTVTGAKGTLVRQFSPVITI
                     NKEDNVITVARSSEQKTHKQLHGTTRALLANMIEGVHEGFKKQLEIVGIGYRGQMKGN
                     TLVLNIGYSHQVEIEAEEGVTIETPNNTTIVVSGISKERVGQVAAQIREVRKPEPYKG
                     KGIKYSDERIIRKEGKTAGKK"
     gene            844..1200
                     /gene="rplR"
                     /locus_tag="PLK68_15070"
     CDS             844..1200
                     /gene="rplR"
                     /locus_tag="PLK68_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRM91769.1"
                     /translation="MSKLQSRNDVRLKRHARVRAKISGTPECPRLNVFRSNAHIHAQV
                     IDDVNGVTLASASSVDMKLENGSNIAAATAVGKAVGEAAIAKNIKKVVFDRGGYVYHG
                     RVKALAEAAREAGLEF"
     gene            1213..>1695
                     /gene="rpsE"
                     /locus_tag="PLK68_15075"
     CDS             1213..>1695
                     /gene="rpsE"
                     /locus_tag="PLK68_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRM91770.1"
                     /translation="MEQRKPRNNGPRNNGPRRGQRNNGPRREEKEFEERVVTINRVTK
                     VVKGGRKFRFAALVVIGDRKGRVGFGTGKANEVPDAIRKASENAKRNVINVPSVHGTI
                     PHEVTGIYGSGRVFIKPASQGTGIIAGGPVRAVVELAGYSDILSKSLGSRTPINMVRA
                     T"
BASE COUNT          626 a          239 c          368 g          462 t
ORIGIN      
        1 gttgagggcg aaggtccaat caaaaacatt aatatcactt taaaatatag aggaaatgat
       61 agagtaatca ctgatttaaa aagaatttct aaaccaggat tacgtgtata tgctaaagta
      121 aacgaaattc ctaaagtatt aaatggttta ggtatcgtaa tcttatcaac ttctcaaggt
      181 ttgatgacag ataaagaagc tcgcgctaaa caagttggtg gcgaagttct tgcatatatt
      241 tggtaataaa aaatacaatt caggaggtgc aaactgatgt ctcgtgtcgg taataaaatt
      301 attaatgtac ctgaaggagt tacagttgat atcgctgctg ataatacagt aactgttaca
      361 ggagctaaag ggactttagt aagacaattt tcaccagtaa ttacaattaa caaagaagat
      421 aatgtaatca ctgttgctag aagtagtgaa caaaaaactc ataaacaatt acatggtaca
      481 acaagagcat tgcttgcaaa catgattgaa ggagttcatg aaggattcaa aaaacaatta
      541 gaaatagttg ggattggata tcgtggacaa atgaaaggga atactttagt gttaaacatt
      601 ggatattctc atcaagtaga gattgaagct gaagaaggag ttacaattga aactcctaac
      661 aatacaacaa tcgttgtatc tggaatctca aaagaacgtg ttggtcaagt tgctgctcaa
      721 attagagaag taagaaaacc agaaccttat aaaggtaaag ggattaaata tagtgatgaa
      781 agaatcatca gaaaagaagg aaaaactgct ggtaagaagt agtttcgaaa ggagcaaaat
      841 aagatgtcaa aattacaatc tcgtaatgat gttcgtttaa aaagacatgc tagagttcgt
      901 gctaaaatca gtggaactcc tgaatgccca cgtttaaacg tgttccgttc aaatgctcat
      961 attcatgcgc aagttattga tgatgttaac ggtgttactt tagcaagtgc atctagtgtt
     1021 gatatgaaat tagaaaacgg ttctaacatc gctgctgcaa ctgcagtagg taaagctgtt
     1081 ggagaagcgg caatcgcaaa gaatatcaaa aaagtagtat ttgatcgtgg tggatacgtt
     1141 tatcatggtc gtgtaaaagc cctagctgaa gctgctagag aagctggatt agaattctag
     1201 aaggaggtca atatggaaca acgtaaacca agaaacaatg gaccaagaaa caacggacct
     1261 agaagaggtc aaagaaacaa tggacctaga agagaagaaa aagaatttga agaacgtgtt
     1321 gttactatta accgtgtaac taaagtagta aaaggtgggc gtaaattccg ctttgctgca
     1381 ttagttgtaa tcggagatag aaaaggtaga gttggtttcg gtactggtaa agcaaacgaa
     1441 gtacctgatg caatcagaaa agcttctgaa aatgcaaaaa gaaatgttat caacgttcct
     1501 tcagttcatg gaactattcc tcatgaagtt actggtattt atggatcagg tagagtattt
     1561 attaaacctg cttctcaagg tactggaatc atcgctgggg gaccagttcg tgctgttgta
     1621 gaattagctg gttatagtga tattttatct aaatctttag gttctagaac acctattaac
     1681 atggttagag ctaca
//
LOCUS       DAOMTA010000736         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3680_length_1694_cov_2.644295, whole genome shotgun sequence.
ACCESSION   DAOMTA010000736 DAOMTA010000000
VERSION     DAOMTA010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_1694_cov_2.644295"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..961)
                     /locus_tag="PLK68_15080"
     CDS             complement(113..961)
                     /locus_tag="PLK68_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91771.1"
                     /translation="MLELINVQKDYGRFKLNCSLEVKAGCVTGLIGQNGAGKSTTFKA
                     VLGLISVDSGTVKLFSKDIKRLSIEDKQNIGVVLSDSGFSEYLRIEDIIPIMESLYRH
                     FDKQMFIERVRHFELPLNKQIKEFSTGMKVKLKVLIALSHQSKLLILDEPTAGLDVIV
                     RDELLDMLRDYMEQNEDCAIIISSHISSDLEGLCDDLYMIDQGKIVMHEETDVLLSDY
                     AILKVDEEQFYELDKQYLLCQKKESFGYRCLTNQKQFYLENYPDVVIEKSSIDEVMTL
                     MIRGEK"
     gene            complement(965..1336)
                     /locus_tag="PLK68_15085"
     CDS             complement(965..1336)
                     /locus_tag="PLK68_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM91772.1"
                     /translation="MKIIINNSSMQPIYEQLVEQIKAQIINGQLHENDVLPSVRALSK
                     ELKISALTVKKAYDHLETEGFTATIHGKGTYVKGANQELLLEERRKEVETELESTIDK
                     GRRYGMNDDEIKALFNLIMEE"
     gene            complement(1462..>1694)
                     /locus_tag="PLK68_15090"
     CDS             complement(1462..>1694)
                     /locus_tag="PLK68_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM91773.1"
                     /translation="VEFRRMVEVKDIEPDVKPVDPDEQTPSDNSGQQTIPGQVVEAAK
                     TSDDVNITGMFGILVLAGLGWIGSRKRKFCDC"
BASE COUNT          535 a          360 c          191 g          608 t
ORIGIN      
        1 aaaaatatat ttgtctgggt aaccgcaaaa aatattgcca ttgctgcaat tacgataaaa
       61 aacatctttt gattcatcat taatttaaaa tctttaatat ataggccttt cattattttt
      121 cacccctaat cattaatgtc attacctcat caatactact tttttcaata accacgtcag
      181 gataattttc taaataaaat tgtttctgat tagttaaaca gcggtatcca aaactttctt
      241 tcttttgaca aagcaaatat tgtttatcta attcataaaa ctgttcctca tcaactttca
      301 aaatcgcata gtcacttaat aatacatctg tttcttcatg cataacaatt ttaccctgat
      361 caatcatata taaatcatca catagtcctt ctaaatcact ggaaatatga gaactaataa
      421 tgattgcaca atcttcattt tgttccatat aatcacgaag catatccagc aattcatctc
      481 gcacaataac atctaagcct gctgttggct catctaaaat caataactta ctttggtgtg
      541 ataaagcaat caaaactttt aattttactt tcattcccgt tgaaaattct ttgatttgtt
      601 tgtttaatgg taattcaaaa tgacgaactc tttcaataaa catctgttta tcaaaatgac
      661 gatacagtga ttccataata gggatgatat cttcaatcct cagatattca ctaaatcccg
      721 aatccgataa cacgacccca atattttgtt tatcttctat acttaatctt ttaatatctt
      781 tgctaaataa tttaacagtc ccactatcta ctgaaatcag acccaaaaca gctttaaaag
      841 tagtactttt accagcacca ttttgtccga tcagcccagt aacacaacca gccttaactt
      901 ctaaagaaca attaagctta aaacgtccat aatctttttg cacattaatt agttctaaca
      961 tatcttattc ctccataatc aaattaaata aagcttttat ctcatcgtcg ttcatcccgt
     1021 aacgtcttcc tttatcaatg gtactttcca gttcagtttc gacttctttg cgccgctcct
     1081 ccaacaacaa ctcttgattc gcccctttta cataagtacc tttgccatga atcgttgctg
     1141 taaatccttc tgtttcaaga tgatcatacg cctttttaac tgttaatgcg cttatcttta
     1201 attcttttga taacgcacga actgatggta atacatcatt ttcatgcaat tgaccattta
     1261 tgatttgagc ttttatttgt tcaacaagtt gttcatagat tggctgcatc gatgaattat
     1321 ttatgataat cttcatcttc tccctcctct tcacaaacag tatataacag cgtataactg
     1381 attgtcaact gttatatgct gttataaaaa aagataagga cccacaatgt gagtccttat
     1441 cgcaaggaga gagagtttta tctaacaatc acaaaatttt ctttttcttg atccaatcca
     1501 tcctaatcca gctaatacaa gtataccaaa catacccgtg atatttacat catcactagt
     1561 cttagctgct tcaactactt gtcctggaat agtctgttga ccactattat ctgatggtgt
     1621 ttgctcatca ggatcaactg gtttaacatc tggttcgatg tccttaactt caaccattct
     1681 tctaaattct actt
//
LOCUS       DAOMTA010000737         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3714_length_1687_cov_2.061275, whole genome shotgun sequence.
ACCESSION   DAOMTA010000737 DAOMTA010000000
VERSION     DAOMTA010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3714_length_1687_cov_2.061275"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLK68_15095"
     CDS             complement(<1..517)
                     /locus_tag="PLK68_15095"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM91774.1"
                     /translation="MSLHRDTYAEINLKYLKENIETTYKKFKRPLMAVIKADAYGHGY
                     REVATYIKDIEYLEMFAVATLPEAIELRELGITKGILILGAVPTSKEEIDLAIKYDIS
                     LTMISLDYMHHLETLIDQQPLKIHIKLDTGMHRIGLTSKAQLDEMLNTIDYNKFILEG
                     IFTHYATADGEQ"
     gene            complement(604..1584)
                     /locus_tag="PLK68_15100"
     CDS             complement(604..1584)
                     /locus_tag="PLK68_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91775.1"
                     /translation="MKKIYIGIAAAVVIVIAALTFFKMKDSSLDKVIEKVKAYDKYAL
                     TCNMEMVENDELKSYLVNVSYLKEKKNDKVELYDKSLNQSQIIVKNPDGVFVLTPTLN
                     QIFKFQSEWPNNSPKPYIYQSLIELLEKGEVEKIKTGYQVKCEVTYPNDSRVVAQEII
                     FDKSLAPKQVTVLDKDEAEIITADFTDFKTDVKLGKDNFNEKKVLEKSTNEYSNVSSE
                     LPLYPVALMGSTLDSEKVSTIDGTTNHILKFTGDKNFTVIETPVTASEEVAVETVSGE
                     VIDLVDGVAFYNDGQLMMMKSGVLCKLYSQDLNKDEMVNVISSMQTSSLK"
BASE COUNT          550 a          309 c          195 g          633 t
ORIGIN      
        1 cctgttcacc atcagctgtt gcataatgcg taaaaattcc ttctaagata aacttattgt
       61 aatcaatagt atttaacatc tcatccaact gtgctttgct agttaagcca atacgatgca
      121 ttcccgtatc taatttaata tggattttca acggctgttg atctatcaat gtttctagat
      181 gatgcatgta atccaaggag atcattgtta aagaaatatc atatttaatt gctaaatcaa
      241 tttcttcctt agaagttgga acagctccta aaattaaaat tcctttagta atacctaatt
      301 cacgtaactc aattgcttcc ggtaaggttg ctactgcaaa catctcaaga tattcaatat
      361 ctttaatata tgtggccact tctcgatatc catgaccata agcatccgct ttaattaccg
      421 ccattaacgg acgtttaaat tttttatatg ttgtttcaat attttctttt aaatatttta
      481 aattaatttc tgcatatgta tcacgatgta aactcatatg acacctccat cttgtctaag
      541 tataatccaa tctaattgat aattcaaata ttttcataat tatgtattag aggcattttg
      601 cctctatttt aaactcgatg tctgcatact actgattaca ttgaccattt catctttatt
      661 taaatcttga gaatacaatt tgcataaaac acctgatttc atcatcatta attgaccatc
      721 attataaaat gccacaccat caacaagatc aattacttca ccacttaccg tttcaactgc
      781 aacttcttca ctagcagtta ccggtgtttc aattactgta aaattcttat ctccagtgaa
      841 ttttaaaata tggttagttg tcccatcaat cgttgaaact ttttcactat ctaaagtgct
      901 tcccattaag gcaactggat acaatggtaa ctcactagaa acattagaat actcattagt
      961 actcttttct aaaaccttct tctcattaaa attatctttt cccaatttaa catctgtctt
     1021 aaaatcagta aaatcagcgg taattatttc agcttcgtct ttatctaaaa cggttacttg
     1081 ctttggcgct aatgatttat caaaaataat ttcttgagct accacccgtg aatcatttgg
     1141 ataagtcact tcacatttaa cttgataacc tgtttttatt ttttccactt cgcctttttc
     1201 taataattca attagagatt gatagatgta tggttttggg gaattattcg gccactcact
     1261 ttgaaattta aagatttgat ttaaagttgg tgttaaaaca aaaacaccat cagggttctt
     1321 aacaatgatt tgtgattgat tcaatgattt atcatataat tctactttat cattcttttt
     1381 ttcttttaaa taactaacat tgactagata acttttaagt tcatcatttt caaccatttc
     1441 catattacaa gtaagtgcat atttatcata agccttaacc ttttcaatca ccttatctaa
     1501 agaactatct ttcattttaa agaaagttaa cgctgcgatt actatcacaa ctgccgcggc
     1561 aataccaata tatatttttt tcaaatttac acccccaata ataaatatgt ttaaaaataa
     1621 atattatgta taaagatatt tctaatggat acactttaat aaggaggatg aagataatga
     1681 atatatt
//
LOCUS       DAOMTA010000738         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3742_length_1679_cov_2.810961, whole genome shotgun sequence.
ACCESSION   DAOMTA010000738 DAOMTA010000000
VERSION     DAOMTA010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3742_length_1679_cov_2.810961"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLK68_15105"
     CDS             complement(<1..774)
                     /locus_tag="PLK68_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HRM91776.1"
                     /translation="MIYEITKKKIKWVKVVTVLIVIAIILKVGYIQIIDRVNIYNKAV
                     DLWQRSFPVEANRGLIVDSDNNVLATNLTTASLVVVPSQIKDVAATADKIANILNVDV
                     KVMQEKLSKKVSIQRIQPEGRQLDDEVAAQIDRLKLPGVYLIKDTKRYYPNDNYLGQT
                     LGFVGIDNQGLLGLELKYDNYLNGNNGSIDYYMDAKSNPLSLYPSVYSAPTSGFNLQL
                     TIDGDIQDIVERELNNAYDTYNPDGIWALAMEPSTGKVLA"
     gene            complement(820..>1679)
                     /locus_tag="PLK68_15110"
     CDS             complement(820..>1679)
                     /locus_tag="PLK68_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM91777.1"
                     /translation="FGQGSTVTALQLLRAYSVFANDGKTVEPYLIEKIVNKETNEVTY
                     KGKTEYSEQIFSSQTVQHVRDLLKGVVTESMGTAKKFNLDNGVQIIGKTGTGQVVVDG
                     GYSSTIYTKSFSGMAPYEDPQIIVNIVFQGADNDTTQHQANVIKSVMPAALSIVNKYN
                     APETTTVSSDYKLDSYVNQSVSFVKSKLESKAVNVQVIGNGNTVLEQYPEAGSKVAKN
                     DRVFIKTESNDIVLPNFTGWSRKDVLTFGSLSGVNITIDGGNGLVGAQSAPEGTVVHN
                     GDALTITLQ"
BASE COUNT          525 a          330 c          228 g          596 t
ORIGIN      
        1 cgctaaaact tttccagtac ttggttccat cgctaaagcc cagatgccat caggattata
       61 agtgtcatat gcattgttca gttctctttc aacaatatcc tgaatatcac catcaatcgt
      121 taattgtaaa ttaaaaccac tcgttggagc actataaaca cttggataca gtgacaacgg
      181 gttacttttg gcatccatat aataatcaat actcccgtta tttccgttta aataattatc
      241 gtattttaat tctaaaccta ataatccttg attatcaatt cctacgaagc caagagtctg
      301 tcctaaataa ttatcattgg gataatatcg tttagtatct ttaattaaat ataccccagg
      361 taattttaaa cgatcgattt gtgctgctac ttcatcatct aattgtcgtc cttctggctg
      421 aattctttgg attgaaactt ttttacttaa tttttcttgc attactttaa catcaacatt
      481 taaaatattt gcaattttat cagcagtagc cgccacatct ttaatttgac taggaacaac
      541 aactagtgaa gcagttgtta gatttgttgc taaaacgtta ttatcactat caacgatcaa
      601 tccacgatta gcttcaactg gaaaactacg ctgccataaa tccactgctt tattataaat
      661 attgactcga tcaataattt gaatatagcc aacttttaaa attattgcaa ttacaatcaa
      721 aactgttact acttttaccc atttgatttt cttttttgtt atctcataga tcatctaatc
      781 accctctaca ctatatgaaa aaagtaaggt cattagacct tactgaagtg taattgttag
      841 agcatcaccg ttgtgaacaa cagttccctc tggcgcactt tgagctccga cgagaccgtt
      901 accgccatca atagtaatat taacccctga taacgaacca aaagttaaaa catctttacg
      961 tgaccagcca gtaaaattag gtaatacaat atcattagat tccgtcttaa taaaaacacg
     1021 atcatttttt gctactttag aaccagcttc tggatattgt tcaagaactg tattaccgtt
     1081 tccgattact tgaacattta cagcttttga ttctaatttt gacttaacaa aactaacact
     1141 ctgattaaca tatgaatcta gtttataatc actagatact gtcgtagttt caggagcatt
     1201 atatttattt acgattgata atgctgccgg cattacactt ttaataacat ttgcctgatg
     1261 ttgagttgta tcattatcag caccttggaa aacaatatta acaattattt gcggatcttc
     1321 atatggtgcc attcccgaaa aggatttggt ataaatagta cttgaataac caccatcaac
     1381 aactacttgt cccgttccgg tcttaccgat aatttgaaca ccattatcaa gattaaattt
     1441 ctttgctgtt cccatacttt cagttactac tccttttagt agatctcgaa catgttgaac
     1501 tgtttgtgaa gaaaatattt gttctgaata ttctgtttta cctttatatg taacttcgtt
     1561 tgtttcttta ttaacgattt tttcaattaa ataaggttca acagtttttc catcatttgc
     1621 aaagacacta tatgcgcgca atagttgtaa agctgtaacg gtagagccct gcccaaatc
//
LOCUS       DAOMTA010000739         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3746_length_1678_cov_1.590881, whole genome shotgun sequence.
ACCESSION   DAOMTA010000739 DAOMTA010000000
VERSION     DAOMTA010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3746_length_1678_cov_1.590881"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLK68_15115"
     CDS             <1..458
                     /locus_tag="PLK68_15115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM91778.1"
                     /translation="SCDVVLASLIQRVKQKNTSFAVAMGDVDFFKYVNDTYGHQAGDE
                     TLRQLAKVISIHMEHLGFVFRWGGEEFVLIYEDMDRYQAFKHLEILQEQIDQESIYWK
                     DDKVKITMTFGLADSNEYNDLDELINLIDDNLYRGKKEGRNRIVFNTLK"
     gene            595..1449
                     /locus_tag="PLK68_15120"
     CDS             595..1449
                     /locus_tag="PLK68_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM91779.1"
                     /translation="MIKLIVSDMDGTLLAHDSSISKGNIEAIRYAQSKGVQFAIATGR
                     DYSSLKGILEAHDLKCFSILGNGAQFCNENGEILSSAYFPKKCFKQVLQIFDELKIHY
                     MIFTANGFYSTAEPNVVRDAFIDRCVVQFKRKREDYLDDGCNQDMACMKLKKIGDLDD
                     FINSSIDIIKVEAFNNDVSLIEKAKEKLQEIEGIAYLSSFDDNIEVTDKAAQKGLILE
                     NVIEELGYSKDEVMVLGDGLNDITLFERFKYSFAPGNANETIKAMAYQVVGACEEDGV
                     SQAIYMML"
     gene            1495..>1678
                     /locus_tag="PLK68_15125"
     CDS             1495..>1678
                     /locus_tag="PLK68_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91780.1"
                     /translation="MIEIKFLKSTDEYWDTMIDYVQNCSWRAGKSLANKMKTGYFTKW
                     QCVVGVWYDNKIVGFST"
BASE COUNT          584 a          167 c          351 g          576 t
ORIGIN      
        1 atagttgtga tgtggtttta gcgagtttaa ttcagcgtgt aaaacaaaag aacacttctt
       61 ttgcagtggc aatgggagat gttgattttt ttaaatatgt taatgataca tatggtcatc
      121 aagcaggtga tgaaacttta cgtcaacttg ctaaagttat ttcgatccat atggaacacc
      181 tgggatttgt atttagatgg ggtggtgaag aatttgtctt gatctatgaa gatatggatc
      241 ggtatcaggc ttttaagcat ttagaaatct tacaagaaca gattgatcaa gagagtattt
      301 attggaaaga tgacaaagtt aagattacaa tgacatttgg cttagcggat tctaatgaat
      361 acaatgatct tgatgaattg atcaatttaa ttgatgataa tctttatcga gggaaaaaag
      421 aaggtcgtaa ccgaattgtt tttaatactt taaaatagaa attaaaacat gaccgttaag
      481 ataattttaa cggtttttta gataaagatt tctaattgag aaaatatctt taatgtttca
      541 aaagattaga tacaatagaa ctataaactt tattaaaggt atgaggtatg aaaaatgata
      601 aaattaattg tatcagatat ggatggaaca ctattggcgc atgattcatc aatttcaaag
      661 ggaaatattg aagcaattag gtatgctcaa agtaaaggag tgcaatttgc aattgctacc
      721 gggcgtgatt attcaagcct taaggggata ttagaggcac atgatttaaa atgttttagc
      781 attttaggaa atggagcaca attttgtaat gaaaatggag aaattttatc aagtgcatat
      841 tttcctaaaa aatgttttaa acaagtttta caaatatttg atgaactaaa gattcattat
      901 atgattttta ctgcgaatgg tttttattca acagctgagc caaatgttgt tcgtgatgct
      961 tttattgatc gctgtgttgt tcagttcaag cgcaaacgag aggactactt agatgatggc
     1021 tgtaatcaag atatggcgtg tatgaagtta aaaaaaatag gtgacttaga tgattttatt
     1081 aattcttcaa ttgatattat taaggttgaa gcatttaata atgatgtatc attaattgaa
     1141 aaagctaaag aaaaacttca ggaaattgag gggatagctt atttatcatc atttgatgat
     1201 aatattgaag ttaccgataa ggcagctcaa aaaggattga ttttggaaaa tgttattgaa
     1261 gaattaggtt attcaaaaga tgaagtaatg gttttaggtg acggtctcaa tgatattaca
     1321 ttatttgagc ggtttaagta ttcttttgcg ccaggtaatg ctaatgagac aattaaagca
     1381 atggcttatc aggtagttgg agcatgtgaa gaagacggtg tttcccaagc aatctatatg
     1441 atgttataat aaagaggatt gataagatct tctttttttg atgggaggaa tttaatgata
     1501 gaaattaaat ttttaaaaag tacggatgaa tattgggata caatgattga ctatgttcaa
     1561 aattgttctt ggcgtgctgg taaatctttg gctaataaaa tgaagacagg gtattttact
     1621 aaatggcagt gtgtagttgg ggtttggtat gataataaaa ttgttggttt ttcgactt
//
LOCUS       DAOMTA010000740         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3755_length_1676_cov_1.936459, whole genome shotgun sequence.
ACCESSION   DAOMTA010000740 DAOMTA010000000
VERSION     DAOMTA010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_1676_cov_1.936459"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLK68_15130"
     CDS             <1..279
                     /locus_tag="PLK68_15130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM91781.1"
                     /translation="DAIISRFGGDEFVVFVKNIQDIKLFNRLISRLLQQLDLYYQSKK
                     DQINIQASIGIALAPIDGRDMNTLYLKADKALYQAKEGGKNRYKYYNN"
     gene            842..>1116
                     /locus_tag="PLK68_15135"
     CDS             842..>1116
                     /locus_tag="PLK68_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM91782.1"
                     /translation="MLNTVALVGRLVDIPEMKTTIGGVKVANATIQIEQGFKNSLGAF
                     ENDYIVVSLWRGIAEMIIDFARPGCMIAVKGRLHSRTFECAESKQITS"
     assembly_gap    1117..1126
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1236..>1676
                     /locus_tag="PLK68_15140"
     CDS             1236..>1676
                     /locus_tag="PLK68_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM91783.1"
                     /translation="MTIDQSKIRNFSIIAHIDHGKSTLADRILQITGAVADREMKAQL
                     LDSMDLERERGITIKLNAVQLNYTAKDGQTYLLHLIDTPGHVDFTYEVSRSLAACEGA
                     ILVVDSVQGVEAQTLANVYLALDNNLEILPVINKIDLPSADPQRV"
BASE COUNT          600 a          196 c          309 g          561 t
ORIGIN      
        1 gatgctatta tttcacgttt tggaggagat gaatttgtgg tttttgtaaa aaacattcaa
       61 gacatcaagc tttttaatcg tttaatatcc agacttcttc aacaattaga tttgtattat
      121 caaagtaaaa aggatcagat aaacatccaa gcttctattg gaatagcact agcacctatt
      181 gatggtagag atatgaatac tttatattta aaagcagata aagcgttgta tcaagcaaaa
      241 gaaggcggaa aaaatagata caagtattac aataattagt tttgtttaga taagatgtta
      301 actaaatatt tagcccaatt taatattaat tgaactctag caaaataaat tggaattcta
      361 aagataaagt atgtacaagc aagatgagtt aaggtactat aatggagcat aatcaaaaat
      421 atatttattt ctgtgtttaa gaaagtataa gaaaatatct tttttatatt gtcctcatta
      481 tgatggggtg ggaataataa aaaatgaatc ttaatttggg aatattaaac tctattttta
      541 tagagttttt tttgttattg gaggtattga aaaatattat ataaaaataa atatagtagt
      601 tattttgggc gaaacaggga taagatggac tttttttgca aaatatgtat atttttttcg
      661 acaatttttt atagactgat atagtctatt taagcatatc tgttaacttt tgctacacat
      721 ctgaaagaaa aagtttcacc agtgacccat tttgacaatg tcgttaatta tggttaattt
      781 tacagtgttg taatcttttt ataattatgc ctataatttt gtcataaagg aggggtaaat
      841 aatgttaaat actgtagcat tagtagggag attagtagat atcccggaaa tgaaaacaac
      901 gattggaggt gtaaaagtag caaatgcaac tattcaaatt gaacaaggtt ttaaaaatag
      961 tcttggtgca tttgaaaatg attacattgt tgtaagttta tggcgcggta ttgctgaaat
     1021 gattattgat tttgcaagac caggatgcat gattgcagtt aaaggaagac tgcattcaag
     1081 aacatttgaa tgtgccgagt caaaacaaat tacttcnnnn nnnnnntgca gaacgagttt
     1141 ctttattaga taaatatttc aaaaactatg attaaatatc atttgagttt aaccggctta
     1201 tttgttataa tgatttaacg aaagtgggtg aaaaaatgac tattgatcaa agtaaaatta
     1261 gaaatttttc aattatcgca catatcgatc atggtaaaag caccctagca gatcgaattt
     1321 tgcaaataac tggagctgtc gcagatcgtg aaatgaaggc acagttactc gatagtatgg
     1381 atttagagcg agaaagagga attaccatca aattaaacgc agttcaactg aattatactg
     1441 ctaaagatgg tcaaacatat ttgttacatt taattgatac accaggccat gtcgacttta
     1501 cttatgaggt atcgagatcg ttggctgctt gtgaaggtgc aatattagtt gttgattcag
     1561 tgcagggagt tgaagcacaa acgttggcaa atgtgtattt ggctctagat aataatttag
     1621 aaatattgcc agttattaat aaaatcgatt tgccaagtgc tgacccgcaa cgagtt
//
LOCUS       DAOMTA010000741         1671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3774_length_1671_cov_1.551980, whole genome shotgun sequence.
ACCESSION   DAOMTA010000741 DAOMTA010000000
VERSION     DAOMTA010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3774_length_1671_cov_1.551980"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>668)
                     /locus_tag="PLK68_15145"
     CDS             complement(<1..>668)
                     /locus_tag="PLK68_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRM91784.1"
                     /translation="EFGARRAQGYDSATYGARAAYIGGVAGTATVSAGMMFNIPVVGT
                     MAHSFVQSFESEFEAFKAYALTYPDDCVLLVDTYDTLKSGVPNAIKVANEVLAPLGKK
                     LKGIRLDSGDIAYLSKRARVMLDVAGLVDAKITASNSLDEYLIRSLLDQGAQLDSFGV
                     GENLIVSRSAPVFGGVYKLVALEKNGKIIPKIKISENTEKITNPGYKKVYRLFENETG
                     KAIG"
     assembly_gap    669..719
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<720..1138)
                     /locus_tag="PLK68_15150"
     CDS             complement(<720..1138)
                     /locus_tag="PLK68_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRM91785.1"
                     /translation="MDLHAGSKRNLTLVMDLYELTMSYNYFKQGNKDEYVYFDMYYRK
                     NPDNGGFSIFAGLQQLIECIEHLHFSEGDIKYLRSLNKFDEEFFDYLRDFHFTGSIYA
                     VKEGTPVFPNEPLITIRAKFIEAQLIETLLLVTVNHQS"
     gene            complement(1204..1506)
                     /locus_tag="PLK68_15155"
     CDS             complement(1204..1506)
                     /locus_tag="PLK68_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRM91786.1"
                     /translation="MYEDYMDNLFDRRPMPTVAPKVGDIFLYTVNRGDNVYAIARRFN
                     TRVDYIKNMNSLDDKMMIYPGQQLLVPVLFEKMPPMKPQPLPQPQPQPRQSYELYF"
BASE COUNT          517 a          332 c          219 g          552 t
ORIGIN      
        1 caccaattgc ttttcctgtt tcattttcga ataagcgata tactttttta tatcctggat
       61 tcgttatttt ttccgtattt tcagaaattt tgattttagg aataattttt ccattttttt
      121 ccagagccac taacttatat acaccaccaa aaactggcgc actccgtgaa acaataaggt
      181 tttcacccac cccaaatgaa tccagctggg ctccttgatc caataaagaa cgaatcaaat
      241 attcatctaa tgaatttgat gctgttatct tagcatcaac taatcccgca acatcaagca
      301 tcactcgcgc tcgctttgat aaataagcaa tgtcaccact gtctaagcgg atgcctttta
      361 attttttacc taacggcgct aaaacttcat ttgctacttt tatagcatta ggcacaccac
      421 tttttaatgt atcatatgta tcaactaata atacacaatc atccggatat gtaagagcat
      481 acgctttaaa agcttcaaat tcactttcaa atgattgaac aaatgaatga gccattgttc
      541 ctactacagg aatattaaac atcatccccg cagaaactgt agctgtcccg gcaactccac
      601 caatataagc agctcgagct ccatatgtag cactatcata gccttgagct cgccttgccc
      661 caaattctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      721 attgatggtt tacagttacc aataataaag tttcaattaa ttgtgcttct ataaattttg
      781 cacgaattgt aattaaaggt tcattaggga aaaccggtgt accttcttta accgcatata
      841 ttgaccccgt aaaatgaaaa tctcgtaaat aatcaaaaaa ttcttcatca aacttattta
      901 gtgagcggag atatttaata tctccctcac taaaatgtag gtgttcgata cattcaatca
      961 actgctgaag accagcaaat attgagaatc caccattatc tggattttta cgataataca
     1021 tatcaaaata cacatattca tctttattgc cttgtttaaa ataattataa gacattgtta
     1081 attcatatag atccattacc aatgttaaat tacgtttact tcctgcatgt aaatccatct
     1141 attgttctcc ttgtatatat ttatcattat aataaacttt tttcttataa aaatctagtt
     1201 attttagaaa tatagttcat aagactgtct tggttgtggt tgcggctgtg gtaatggctg
     1261 tggcttcatt ggtggcattt tttcaaatag tactggaact aataattgtt gcccaggata
     1321 gatcatcatt ttatcatcta ggctattcat atttttaata tagtcaactc gtgtattaaa
     1381 tcgtcgtgca attgcataaa cgttatcacc acgatttaca gtatatagaa agatatcacc
     1441 tacctttggt gcaactgttg gcattggtcg ccgatcaaac aaattatcca tgtaatcttc
     1501 gtacatgtac atccctccta ccataaccta ttcaaataaa aaaataataa ataggcaaaa
     1561 aaacagactt cataaaaaag tccgttatct tgctaattta tttaatgcat ctttagctgc
     1621 ctgttgctct gctcttttct tcgaagaacc tactccaacc cctaatacca t
//
LOCUS       DAOMTA010000742         1662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3805_length_1662_cov_2.569384, whole genome shotgun sequence.
ACCESSION   DAOMTA010000742 DAOMTA010000000
VERSION     DAOMTA010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_1662_cov_2.569384"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLK68_15160"
     CDS             <1..1178
                     /locus_tag="PLK68_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91787.1"
                     /translation="VISEVFAQGYMQVLIGTKSLLGEGWDSPCVNSLILASFVGSYML
                     SNQMRGRAIRVFEKTPNKTSNIWHLVCVRPKEKLTGHYDDGGSEDFQTLARRMEHFLG
                     LHYQQDIIENGILRLSAIKLPFSPGNIKKINKDMLKLSSKRSQLKKRWEDSLAVYDKI
                     EIVEETEVKEEFITVVMFMDALRTLLIIVFSGIIGAVIGIPFLKSLTLIDILEYLVVI
                     IYVGIFVMSILLSIKKLYTLANPLGRLEIFGKGIRNALEKTNQLDSLNSRVETDSFNA
                     IHAIYLLGGTGHDKALFAKCINEFFASIDNQRYILYNPKRKNKLDCYFAIPDSFAKRK
                     EDAHIFAGYMKPFIGNYQVIYTRNESGRKILLEARVSALANRQDRCFTRKKVKGALE"
     gene            1250..>1662
                     /locus_tag="PLK68_15165"
     CDS             1250..>1662
                     /locus_tag="PLK68_15165"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRM91788.1"
                     /translation="MSYIELDEVDSTNDYVKRNLNKLPNLSVVRCNYQTNGRGRNGHV
                     WQSKNGDDLLMSILVKDFKKPQDLHKMTQLVACSVAGLLDRYGIKAKIKWPNDIYVDD
                     LKICGILVEAIYQTDLEGVVVGVGLNVNSINGEYAS"
BASE COUNT          566 a          188 c          347 g          561 t
ORIGIN      
        1 gggtaatcag tgaggtattt gcgcagggtt atatgcaagt tttgatagga actaaatctt
       61 tacttggaga aggttgggat tcaccgtgtg ttaattcgct tattttggct agctttgtag
      121 gctcatatat gttaagtaat caaatgcgtg gacgagctat tagagttttt gagaaaacgc
      181 ccaataaaac aagtaatatt tggcatttag tttgtgttag accaaaagaa aagttaacgg
      241 gtcattatga tgatggcggt agtgaagatt ttcaaacgct tgcaaggaga atggaacatt
      301 ttttagggtt gcattatcag caggatataa ttgaaaatgg aatcttgcga cttagcgcaa
      361 ttaaattgcc atttagtcca ggaaatataa aaaaaattaa taaagatatg cttaaactat
      421 cttcaaagcg tagtcaatta aagaaaagat gggaagattc gttagctgtc tatgacaaaa
      481 ttgaaattgt tgaagaaact gaagttaaag aagaatttat aactgtcgtt atgtttatgg
      541 atgctttaag aactttactg ataattgttt ttagtgggat tattggtgct gtcattggaa
      601 taccatttct aaaatcattg accttgatag atattttaga atatctagtg gtgataattt
      661 atgttggaat atttgttatg agtattcttt tgagcatcaa aaaactttat acattagcaa
      721 atcctttagg tcgtttagaa atatttggca aaggaattag aaatgcgtta gaaaaaacca
      781 atcagctgga ttctttgaat agtcgcgttg aaacagattc ttttaatgcg atacatgcaa
      841 tatatttatt agggggaaca gggcatgata aggctttgtt tgccaaatgc atcaatgaat
      901 tttttgcatc tattgataat caacgttata ttctttataa tcctaaacga aaaaataaat
      961 tagattgtta ttttgcaatt cctgatagtt ttgctaaaag aaaagaagat gcgcatatat
     1021 ttgctggcta tatgaagccg tttattggta attatcaggt tatttatact cgtaatgaaa
     1081 gtggtagaaa aatactgcta gaagcccggg ttagcgcttt agctaatcgt caagatcgtt
     1141 gtttcactag aaaaaaagta aagggtgcat tagaataaaa ctttataata aatatttgtt
     1201 gttttcctaa atttctaata taatagaagt atagtaatgg ggtgatatta tgagttatat
     1261 tgaattagac gaagtcgatt ctacaaatga ttacgtaaaa cgtaatttaa ataaacttcc
     1321 taatctaagt gtagttcgtt gtaattatca aacaaatggt cggggtcgga atggacacgt
     1381 ttggcaaagt aagaatggtg atgacttgct gatgagtatt ttggtaaaag attttaaaaa
     1441 acctcaagac cttcataaaa tgacacagct agttgcttgt agtgtggcgg ggttactaga
     1501 tcgttatgga ataaaagcta aaattaagtg gcccaatgat atttatgttg atgatttaaa
     1561 gatttgtgga atcttagtag aggcaatcta tcaaactgat ttagaagggg ttgtagttgg
     1621 agttggatta aatgttaatt caataaacgg tgagtatgct tc
//
LOCUS       DAOMTA010000743         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3811_length_1661_cov_2.290162, whole genome shotgun sequence.
ACCESSION   DAOMTA010000743 DAOMTA010000000
VERSION     DAOMTA010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3811_length_1661_cov_2.290162"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..903)
                     /locus_tag="PLK68_15170"
     CDS             complement(172..903)
                     /locus_tag="PLK68_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91789.1"
                     /translation="MRTWAFAKRTTKEILRDPINIIFGLGFPIVILLLLTTIQKNIPA
                     TPFSLKQLTPGIAVFGLSFLSLFSATLISRDRMSSLLARLFTTPMTAKDYILGYTLPL
                     IPMALIQTLLCYLAAFCLGLKITPDVIIAILCTIPISIIFIAIGLFCGTIFNDRQVGG
                     ICGALLTNLTAWLSGTWFSLDLIGGLFKDIAYCFPFVHAVDMARDGLNANYHAMPNNL
                     FWVLSYAGLLLIAAVSLFKYKMKHN"
     gene            complement(881..1630)
                     /locus_tag="PLK68_15175"
     CDS             complement(881..1630)
                     /locus_tag="PLK68_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter A family member"
                     /protein_id="HRM91790.1"
                     /translation="MEMIEIKNLTKKYNDKLAVDNLNLSIKQGKLLALLGVNGAGKTT
                     TLKMLSGLIQPTSGDARINNYSIVNESLFVKELIAVSPQETAIAPNLTVRENLELMAE
                     IHGFKKTLINNKVIEMITAFNLKDVAAKKSKKLSGGYQRRLSIAMALISEPQILFLDE
                     PTLGLDILAREQLWRTIKALKGKITIILTTHYLEEAEALSDYVCIMKDGKIKALGTTN
                     ELIKFAKTTSFEDAFIKLATGGTENENMGLC"
BASE COUNT          545 a          269 c          279 g          568 t
ORIGIN      
        1 atagattgta tcatcgctat ttcgatgaaa ttattgatct tcctgttact aagttaccat
       61 caagttcacc agctaatgcg agttattctt tgacgaaatt aattagtttt tggaaagaaa
      121 taaaatcgtt ataatatatt aatagaaagc aaaaaactca aaaagagttt tttaattatg
      181 tttcatttta tatttaaata gtgaaacagc tgcaattaag aggagtccag cgtagcttaa
      241 aacccagaat aaattattag gcattgcgtg ataattcgca tttaagccat ctcgtgccat
      301 atctacagca tggacaaaag gaaaacaata cgcaatatct ttaaacaagc ctccaattaa
      361 atctaaacta aaccatgtcc cacttagcca cgcggtaaga ttagttaata aggcaccaca
      421 gattcctccg acttgtctat cattaaaaat agtgccacaa aataagccga ttgcaataaa
      481 aataattgaa atggggattg tacataagat ggcaataatg acatcaggtg ttatttttaa
      541 acctagacaa aatgctgcta gatagcaaag taaagtctgt atcaaggcca ttggtattaa
      601 tggtaaagta tatcccaaaa tataatcttt agctgtcata ggagtagtaa ataatcgtgc
      661 taataatgat gacattcgat cacgagagat caatgtagct gaaaataatg ataagaagga
      721 caagccaaat actgcaattc ctggtgttaa ttgttttagt gaaaaagggg tggctggaat
      781 atttttttgg attgttgtca atagtaacaa gataacgatt ggaaaaccta aaccaaaaat
      841 aatattaata gggtcacgta aaatttcttt tgtggttctt ttagcaaagg cccatgttct
      901 cattttcagt tcctcctgtt gcaagtttta taaaggcatc ttcaaagctg gttgtcttgg
      961 cgaatttaat tagttcattt gtggttccca gggctttgat tttaccatct ttcataatac
     1021 aaacataatc tgataaagct tcggcttctt cgagataatg ggtagttaaa atgatagtaa
     1081 tcttaccttt tagtgctttt attgtgcgcc ataattgttc tctggctaaa atatcaagtc
     1141 ctagtgttgg ctcatctaaa aataaaattt gtggctcact aattagggcc atagcaatac
     1201 ttaagcgccg ttgataacca cccgatagtt ttttactttt tttagcagca acatctttca
     1261 aattaaaagc ggtaatcatt tcgatgactt tgttattgat taaagttttt ttaaaaccat
     1321 ggatttccgc cattaattcc aaattttctc taacggttaa attcggtgcg attgcagttt
     1381 cttggggtga aacggcgata agctctttaa caaagaggct ttcattaaca atgctatagt
     1441 tattgattct agcatcaccg cttgttggtt gaatcaaacc cgataacatt ttgagtgtag
     1501 tagttttacc ggctccattt actcccaata gagctagtaa ttttccttgt ttgattgata
     1561 gatttaaatt atcaacagca agtttatcat tatatttttt tgttaaattt ttaatttcaa
     1621 tcatttccat gataatcagt ccttttcatt tttgggtact c
//
LOCUS       DAOMTA010000744         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3816_length_1660_cov_1.661682, whole genome shotgun sequence.
ACCESSION   DAOMTA010000744 DAOMTA010000000
VERSION     DAOMTA010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_1660_cov_1.661682"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLK68_15180"
     CDS             <1..492
                     /locus_tag="PLK68_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRM91791.1"
                     /translation="QKINQTYPKMDYYLHCGDSSLKKDDFLLNKYYTVKGNHDDEDFP
                     VNIILEIGKYRCLITHGNAYDIYYGNDKIKQYMIANNIDICFHGHTHVPAYTQIGNRY
                     IINPGSVMINRGSYGFGTFAIVEIGDTIKVNYYNSETFEECSKMVLDDGKIVLEEFKQ
                     ILK"
     gene            complement(502..846)
                     /locus_tag="PLK68_15185"
     CDS             complement(502..846)
                     /locus_tag="PLK68_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM91792.1"
                     /translation="MMIDKDLAARLKSYRNFKHLTQKDVAAYLKVPHSAISDIENGKR
                     DITVGELRIFSNLYGRSVEEIMSGKKYDYYNIANIARLLTELPDEDLKEVMFMIEYKR
                     KRNEEKNRSEKF"
     gene            complement(958..>1660)
                     /locus_tag="PLK68_15190"
     CDS             complement(958..>1660)
                     /locus_tag="PLK68_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM91793.1"
                     /translation="YYFPINEELDEAISLAHSVNKEIIPFTSFLNNQDILIKFKNSVS
                     YNKINSILVGDYGALQIFKDKKCILDSTFNLYNSYALNYFNNHDAILSLEMSRKQVNH
                     LNNIKQNIIMTVYGKTINMHLKHCLISDHYFNCKKIKCNLCKQGKFTLIDRKGEQFDI
                     FPDQDCNNLIFNSHCLYIDHLEKLEVDFILLSFSNEAPEITKAVFRDFKNNIMFAKPR
                     QISLKTKLTNGYFYD"
BASE COUNT          580 a          231 c          210 g          639 t
ORIGIN      
        1 caaaaaatta atcaaacata tcctaagatg gattattatt tacattgtgg agattcatcc
       61 ttaaaaaaag atgatttctt gttaaacaaa tattatactg taaaaggtaa tcatgatgat
      121 gaagattttc cagtcaatat tattttagaa attggaaaat atcgctgttt aattactcat
      181 ggaaatgctt acgatatata ctacggaaat gataaaataa agcaatatat gattgctaat
      241 aatattgata tctgttttca cggacatact catgttcctg catataccca aattggtaac
      301 cgttatatta ttaatccagg cagtgtgatg atcaatcgag gttcatatgg tttcggaacc
      361 tttgcaatcg tggaaatcgg tgatactata aaagtaaatt attataatag tgaaactttt
      421 gaagaatgct caaaaatggt tctagacgat ggaaaaatcg ttttagaaga atttaagcaa
      481 atactaaaat agaagtgaga tttaaaattt ctcacttcta tttttttctt catttcgctt
      541 acgcttatat tcaatcataa acattacttc ttttaaatct tcatccggta actctgttaa
      601 caaccgtgcg atattagcaa tattatagta gtcgtatttt ttaccactca ttatttcttc
      661 tacacttcgt ccatataagt tagaaaatat tcttaattcc cctactgtaa tatccctctt
      721 accattttca atatctgaaa tggctgaatg tggtactttt aaataagcag caacatcctt
      781 ttgtgtcagg tgtttgaaat tccgataaga ttttaatcgt gccgctaaat ctttatcaat
      841 catcattata cctccgataa atagatttta gcattttcct tatttttctt caataagatt
      901 ttgtatattt ataatttatt tcgacaaata ataattttta tttatataat tagataatta
      961 gtcataaaaa tatccattag ttagttttgt ttttaaacta atttgtcgag gtttagcaaa
     1021 cataatatta tttttgaaat ccctaaatac agctttggtg atttctggtg cttcattact
     1081 aaatgaaagc aaaataaaat caacttctag tttttctaaa tgatcaatgt aaagacagtg
     1141 actattgaat attaagttat tacagtcttg atctgggaaa atatcaaatt gttcaccttt
     1201 acgatcaatt aaagtaaact ttccttgttt acaaagatta catttgattt ttttacaatt
     1261 aaaataatga tcacttatta agcagtgttt tagatgcata ttgattgttt tgccatacac
     1321 tgtcattata atattttgtt taatattatt taaatgatta acttgttttc tactcatctc
     1381 gagagaaaga atagcatcat gattattaaa ataattcaaa gcatagctat tataaaggtt
     1441 gaaagtacta tctaaaatac attttttatc tttaaaaatt tgtaatgctc catagtcacc
     1501 tactaaaata ctatttatct tattgtatga gacactattt ttaaacttga ttaaaatatc
     1561 ctgattattc aaaaagcttg taaatgggat tatttcttta tttacagaat gcgctaaact
     1621 tatagcttca tcaagttctt cgttgatagg aaaataatat
//
LOCUS       DAOMTA010000745         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3819_length_1659_cov_2.579800, whole genome shotgun sequence.
ACCESSION   DAOMTA010000745 DAOMTA010000000
VERSION     DAOMTA010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_1659_cov_2.579800"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..>1659
                     /locus_tag="PLK68_15195"
     CDS             268..>1659
                     /locus_tag="PLK68_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM91794.1"
                     /translation="MKNWWQKSVVYEIYVRSFKDTNHDGIGDINGITEQLDYLATLGV
                     DVLWITPIYESPNDDNGYDISDYYEIMQCFGNMTDFENLLTQAHKRNIKIVMDLVVNH
                     TSDEHRWFIESKKSKDNPYRDYYIWKDGKEDGSVPNNWTSCFLGSAWQYDETTKQYYL
                     HLFSKKQPDLNWDNTDVRNEIQKMIAWWLDKGIDGFRMDVINLISKDQDNIYQDSPIK
                     GHSVSANGPRVHEYIRELTDNVFSKYDVMTVGEAPAVTTKEAIQYASNDGKEMSMVFQ
                     FELMDVDGGEKQKWSDERFKLTDVKAILRKWQIDMHGRAWNSLFWNNHDQPRVVSRFG
                     DTSSKETWEKSAKMLATAQYFLQGTPYIYQGEELGMTNVEFKNIGELRDIESINSYHQ
                     YVEVEKMFTPEEMLRFINKSSRDHARTPMQWNDLENAGFSDAEPWIKVNQNYKWLNAQ
                     AQITDENSIFNYYK"
BASE COUNT          598 a          176 c          340 g          545 t
ORIGIN      
        1 atttaaacta gagttagctc tagttttttt ataatgttta aagtatttat aataatggat
       61 ataaatgtaa ttgtgatcag tcttacgagt ttgctagggg ctttttataa gttatttaga
      121 acttacaaga tattataatg ataacgcttg ctttttctga ggaaaaagat gtttataata
      181 atatttatgg tataattatg atgttagtat tattaattgt ttcgtttata ttgggctata
      241 gtttaggatt tgaggaggaa taataaaatg aaaaactggt ggcaaaagag tgttgtgtat
      301 gaaatatatg tgcggagttt taaagatacc aatcatgatg ggatcggtga tattaatggg
      361 attactgagc agttagatta tttagcaaca ttgggagttg atgtgttgtg gatcacgcca
      421 atttatgaat cgccaaatga tgataatgga tatgatatct cagactacta tgagataatg
      481 caatgttttg gaaatatgac tgattttgag aatcttttaa cgcaagctca taagcgaaat
      541 attaagatag ttatggatct tgttgttaat catacctctg atgaacatag atggtttatc
      601 gaaagtaaga aaagtaaaga taatccatat cgtgattatt atatttggaa agatggtaaa
      661 gaagatggca gtgtaccaaa taattggaca agttgttttc taggttcagc ttggcagtat
      721 gatgagacaa cgaaacagta ttatttacat ttattttcta aaaaacaacc ggatttaaat
      781 tgggataata ctgatgttag aaatgaaatt caaaaaatga ttgcctggtg gcttgataaa
      841 ggaattgatg gatttagaat ggatgtcatt aatttgattt ctaaagatca agataatatt
      901 tatcaagata gtccaataaa ggggcatagt gtcagtgcta atggacctcg tgtccatgaa
      961 tatattcgtg agttaacaga taacgttttt tcaaaatatg atgtgatgac tgttggagaa
     1021 gctccagcag tgacaacaaa agaagctatt caatatgcaa gtaatgatgg aaaagaaatg
     1081 agtatggttt tccaatttga attaatggat gttgatggtg gtgaaaaaca aaaatggagt
     1141 gatgaaagat ttaaattaac ggatgtaaaa gcaattttaa gaaaatggca aattgatatg
     1201 catggacgag catggaattc gttattttgg aataatcatg atcaaccacg agtagttagt
     1261 cgttttggag atacaagttc taaagaaact tgggaaaaat cagcaaaaat gcttgctact
     1321 gcccaatatt ttttacaagg gactccttat atttatcaag gtgaagaact tggaatgact
     1381 aatgttgagt ttaagaatat tggtgagctt agagatattg aaagtataaa ttcttatcat
     1441 cagtatgttg aagtggaaaa aatgtttacc cctgaagaga tgcttcgatt tattaataaa
     1501 tcaagtcgtg atcatgcacg tactccaatg caatggaatg atctagaaaa tgcaggattc
     1561 tctgatgctg aaccatggat caaagttaat caaaattata aatggcttaa tgcacaagca
     1621 caaataactg atgaaaactc aatttttaat tactataaa
//
LOCUS       DAOMTA010000746         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3820_length_1659_cov_2.144015, whole genome shotgun sequence.
ACCESSION   DAOMTA010000746 DAOMTA010000000
VERSION     DAOMTA010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3820_length_1659_cov_2.144015"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1060)
                     /locus_tag="PLK68_15200"
     CDS             complement(5..1060)
                     /locus_tag="PLK68_15200"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HRM91795.1"
                     /translation="MSIKISRKQIRYALVFLFIWILVGFNTGNADSTQYVAMFNAATN
                     GNFYYAEKGFLLLCMLFGKAGFDYQVFLAMYSFVALVLISSFVKENTRYPALALTLYG
                     IFPFLFDAVQIRNLMVEALFLFSIKFLKEKTLKNAIMYTLFILLAATQHTIAIIYLVF
                     LLVYVKEKRKVALISIVGSLSFVVLYRTYLGTIISLFTSNRHNYAFADAATSLSTVIK
                     YFLYFGVLVLIGIFVSRREKHYRATLMKQTVNGVSQEDEFWEKIMLISICLITFIIVD
                     GNYTRLFRNCLIIYYCRILNVNTYRTYRDNKIIIAVIIAFAIVGWLMNFAPGIYYYDT
                     ITNPILHNNIVFGWFLK"
     gene            complement(1063..>1659)
                     /locus_tag="PLK68_15205"
     CDS             complement(1063..>1659)
                     /locus_tag="PLK68_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91796.1"
                     /translation="NTSYSNVGIVGPKIETPDSESEEFARSKLTLASFVKTKKPFCYF
                     RIKANKKGRYQKIKYDNNNIYPFKGMVSGCCLMMNSRLFSENNGFDEKLFLYGEEDVW
                     AYKLEKLGLDAAICGNASVFHNHHNSIKKKGLAFTRFYQWLSPLIVIREYGTYSKALF
                     NIICLINIVTWDLCSIISSDYRRFNHQFRKKIIQLMKG"
BASE COUNT          588 a          316 c          188 g          567 t
ORIGIN      
        1 atcttcattt taaaaaccat ccaaatacaa tattattatg taaaattggg tttgtaattg
       61 tatcatagta atatatacct ggagcaaaat tcattaacca cccaactatc gcaaatgcaa
      121 taattacagc aataattatt ttattgtctc tataagttct ataagtatta acatttaata
      181 tcctgcaata atatattatg agacaatttc taaataatct tgtataattt ccatctacaa
      241 ttataaatgt tatcaagcag atacttatca acattatttt ttcccaaaac tcatcctctt
      301 gactgacacc attaacggtt tgtttcatta gagttgctct atagtgcttt tctcttctac
      361 ttacaaatat tccaatcaaa accaatacgc caaaatatag aaaatatttg attaccgtac
      421 tcaaagatgt agccgcatct gcaaacgcat agttatgcct atttgatgta aacaggctaa
      481 taattgttcc aagataagtc ctgtataaaa caacaaaaga aagacttcca acaatcgata
      541 ttaatgctac ttttcttttt tctttcacgt ataccagcaa aaataccaaa taaataatcg
      601 caattgtatg ttgtgtagct gctaacagaa taaatagtgt gtacattata gcgttcttta
      661 gcgttttttc ctttaaaaac ttaatagaaa acaagaacag tgcctctacc attaaattac
      721 gaatctgtac agcgtcaaat aagaacggaa aaattccata taacgttaat gctaatgctg
      781 gatacctcgt attctccttt acaaaacttg aaattagtac aagcgcaaca aaactgtaca
      841 ttgcaagaaa cacttgataa tcaaatccag ccttaccaaa taacatgcaa agcaatagaa
      901 agcctttctc agcataataa aaattgccgt ttgtagctgc gttaaacatt gccacatact
      961 gagtgctgtc tgcattccct gtattaaacc ctaccaaaat ccatatgaat aaaaacacta
     1021 atgcatacct tatttgtttt ctcgatatct ttatgctcat aattaacctt tcattagttg
     1081 aataatcttc tttctaaatt gatgattaaa tctcctataa tcactagaaa taatggaaca
     1141 caaatcccat gtcacaatat ttataaggca aataatatta aatagcgcct tagagtaggt
     1201 tccatattct cttataacaa ttaggggact tagccactga taaaatcgtg taaaagctaa
     1261 acctttcttt ttaatactat tatgatgatt atgaaaaact gaagcgtttc cgcatattgc
     1321 tgcatctagt ccaagctttt ctaacttata tgcccaaaca tcttcttccc catataaaaa
     1381 tagtttctca tcaaatccat tattttctga aaacaaccta gaattcatca ttaaacaaca
     1441 tcctgaaacc attcctttaa atggatagat attattatta tcatacttta ttttttggta
     1501 tcgacctttt ttattagcct ttatacgaaa ataacaaaat ggtttttttg tttttacgaa
     1561 agatgccaac gttaactttg atctcgcaaa ctcttcactt tcagaatctg gtgtttctat
     1621 ttttggtcca acaataccca cattactata actcgtatt
//
LOCUS       DAOMTA010000747         1655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3830_length_1655_cov_2.343750, whole genome shotgun sequence.
ACCESSION   DAOMTA010000747 DAOMTA010000000
VERSION     DAOMTA010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3830_length_1655_cov_2.343750"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLK68_15210"
     CDS             complement(<1..180)
                     /locus_tag="PLK68_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HRM91797.1"
                     /translation="MKNKYYRINKILNNNAIEILENGTEIIIIGNGVGFNHKVNDYIT
                     MKENYKLYTLQNNLLK"
     gene            complement(200..1648)
                     /locus_tag="PLK68_15215"
     CDS             complement(200..1648)
                     /locus_tag="PLK68_15215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM91798.1"
                     /translation="MIRFPKDFLWGGATACFQYEGGYKEKGRGLSSHDFETAGDIDKE
                     RQITLKLNNGTRGSIDYRDSIPDGAIPYIYDDIYYPSHQATDFYHHWKEDIALFAEMG
                     FKVYRFSISWSRIFPNGDEEKANEDGLKFYEMIIDELLKYNIEPMITICHDEVPFHLC
                     ELYDGWAGRETIGCYVNYATTLFNRFKDKVRYWITFNEINNLGGYAQMGTHKQDSQTR
                     YQAVHHMFVASAKAISQGKKIMGDIKFGAMFALSEIYPATCNPEDVFATYCKRREALF
                     FVDVMARGSYPNYTKDIFNKKDVKLKVSEEDRNLIKKHTLDFISFSYYRSSIVSAGSK
                     VDHMGGEPNPYLKKTQWGAGIDPLGIRYCLNEIYDRYQKPLFVIENGMGAVDILENGK
                     IHDYYRIKYVAEHLKQLRDAIVIDKVPCFGYTMWGCTDLISLASGQMKKRYGFIYVDM
                     NDYGQGSLQRYRKDSFYWYKKVIKTNGSDLTF"
BASE COUNT          531 a          324 c          178 g          622 t
ORIGIN      
        1 tttcaataaa ttattttgta acgtataaag cttgtaattt tctttcattg ttatataatc
       61 attaacttta tgattaaatc ctacgccatt accaataata attatttctg ttccattttc
      121 taatatttca atggcattat tatttaagat tttattaatt ctatagtatt tatttttcat
      181 atctaatcct ctacatttat taaaatgtta aatcagaccc gtttgtttta attaccttct
      241 tataccaata aaatgaatct tttctataac gctgtaatga tccttgtcca taatcattca
      301 tatcaacata tataaaacca tagcgtttct tcatttgtcc actagctaaa gatattagat
      361 ctgtacatcc ccacatagtg tatccaaaac atggtacttt atcaatgaca atagcatctc
      421 tcaattgttt taaatgttca gccacatatt ttattctgta ataatcatga atttttccat
      481 tttctaaaat atctactgct cccattccat tttcaataac aaataatggt ttttgataac
      541 gatcataaat ttcatttaaa caatagcgta tacctaatgg atctattcct gcaccccatt
      601 gggtcttttt taaatacgga ttgggttccc cacccatatg atcaacttta gaaccagcac
      661 taacaattga agaacgataa taactaaatg aaataaaatc taaagtatgt tttttaatca
      721 agtttcgatc ttcctcactt actttcaatt tgacatcttt cttattaaaa atatcttttg
      781 tataatttgg atatgacccg cgagccatta catcaacgaa aaataatgct tcacgtcgct
      841 tacaataagt ggcaaaaaca tcttcaggat tacatgtagc tggataaatt tcgcttaatg
      901 caaacattgc tccgaatttt atgtctccca taattttctt tccctgacta attgctttag
      961 cacttgctac aaacatatga tgaactgctt gatatctcgt ctgactatct tgtttatggg
     1021 ttcccatttg agcgtaccca cctaaattat ttatttcatt aaaagtaatc caatatctta
     1081 ctttatcttt aaatcggtta aatagtgttg ttgcataatt tacatagcat ccaatcgttt
     1141 ctcgccccgc ccaaccatca tataattcac ataaatgaaa gggtacttca tcatgacaaa
     1201 ttgtaatcat tggttcgata ttatatttta aaagttcatc aattatcatt tcataaaatt
     1261 tcaaaccatc ttcatttgct ttttcttcat caccatttgg aaaaattcga ctccatgaaa
     1321 tagaaaaacg ataaacttta aatcccattt ctgcaaacaa agcaatatct tctttccaat
     1381 gatgatagaa atcagtcgct tgatggctcg gataatatat atcatcataa atatacggta
     1441 tagctccatc gggaattgag tctcgataat caatactccc cctcgttcca ttatttaatt
     1501 ttaaagtaat ttgtctttct ttatctatgt caccagcagt ttcaaaatca tgactcgata
     1561 agcctcttcc tttttcttta tatccacctt catattgaaa acaggctgta gcacctcccc
     1621 ataagaaatc ttttggaaac ctgatcattt tctta
//
LOCUS       DAOMTA010000748         1650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3845_length_1650_cov_2.560502, whole genome shotgun sequence.
ACCESSION   DAOMTA010000748 DAOMTA010000000
VERSION     DAOMTA010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_1650_cov_2.560502"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLK68_15220"
     CDS             complement(<1..1322)
                     /locus_tag="PLK68_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91799.1"
                     /translation="MTVLKSLFAYAGKYKYLTILSLVFSMISAILLLMPFIMIWKVIE
                     VILAEYPNFAQGSIAIQYGWYALIFAAVGILLYVSGLLCSHLAAFRIASNMRKKALHH
                     AVQLPLGYFAKEGSGKLRKIIDEAAASTETYLAHQLPDMAQLITTVCAVIVCLFIFDW
                     KFGIAALIPTILALSNMFKMIGPGLQESMKAYMDALEGMSNEAVEYIRGIPVVKTFQQ
                     TVFSFDRFHQSINNYEKFAIGYTNKMRFPMTLFTTFINSIFIFIIGTMMLLILNGFDL
                     NSLMPDFLFYVIFTPIIAVTTNKIMFASENTMMAADALNRIEGIINQKPFTYKHSLQE
                     ITNSDIEFEHVSFTYPESENEVIHDVNLKIKSGSIVAFVGKSGGGKSTLVSLIPRFYD
                     VTKGTIKIGGVDVKEISEKELMSKISFVFQDSRLLKKSFKENIKMGSNA"
     gene            complement(1406..>1650)
                     /locus_tag="PLK68_15225"
     CDS             complement(1406..>1650)
                     /locus_tag="PLK68_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91800.1"
                     /translation="INIHTFRKAFKEIHGKPIYQWYKEYRLEYSLGLLINTDIPIIEI
                     ANEIGYSNPSKYSAAFYQYTSMTPQQYRKSHLKMEQ"
BASE COUNT          584 a          284 c          220 g          562 t
ORIGIN      
        1 gcatttgatc ccatcttaat attttcttta aaacttttct ttaaaagacg actgtcttga
       61 aatacgaatg aaatcttact cattaattct ttttcactaa tttctttaac atcgacacca
      121 ccaattttaa tagtcccttt agtcacatca taaaatctcg gtattaggga tactagcgtt
      181 gatttaccac ctccactttt accaacaaaa gcaacaatag aaccactttt tattttcaaa
      241 ttaacatcat gaatgacttc attttcactt tctggataag taaaagagac atgctcaaat
      301 tcgatatcac tattggtgat ttcttgcaga ctatgcttat acgtaaacgg cttttgatta
      361 ataattcctt cgattcggtt taaagcatct gcagccatca ttgtattttc acttgcaaac
      421 ataattttat tagttgttac tgcaataatt ggtgtaaaaa taacataaaa taagaaatct
      481 ggcattaaag aatttaaatc aaaaccgttt aagatcaata acatcattgt gccaataatg
      541 aaaatgaaaa tactattgat gaaggtcgta aacaaagtca ttgggaaacg catcttattt
      601 gtataaccaa ttgcaaattt ttcataatta ttaattgatt gatgaaaacg gtcaaaagaa
      661 aaaactgtct gttgaaatgt tttaacaaca ggtatcccac gaatgtattc aactgcttca
      721 ttactcattc cttcaagtgc gtccatatat gctttcattg attcctgtaa gccaggacca
      781 atcattttaa acatatttga tagtgctaaa atcgttggaa ttagtgcagc tatcccaaac
      841 ttccaatcaa aaataaataa acagactatt accgcacaaa cagtagtaat caattgtgcc
      901 atatccggta attgatgtgc taaataagtt tctgttgaag ctgcagcttc atcaataatt
      961 tttcttaatt taccacttcc ttctttagca aaatatccaa gtggtaattg aacagcatga
     1021 tgtaaagctt ttttacgcat atttgaagca attctaaatg cagctaaatg tgagcaaagt
     1081 aagcccgaaa catacaataa aattcctact gcagcaaaaa ttaacgcgta ccagccgtat
     1141 tgtatcgcaa tacttccttg ggcaaagttt ggatactctg ccaaaataac ctcaataact
     1201 ttccaaatca taataaatgg cattaataat aaaatagcac ttatcattga aaatactaaa
     1261 gaaagtattg ttaaatattt atatttgcca gcataagcaa ataaagattt taatactgtc
     1321 atattaaccc tcctttagtt agaatagact aactttataa gagagtataa ctcctaaata
     1381 ttattactgc tactccattt tgttattatt gctccatttt aagatgtgat ttacgatatt
     1441 gctggggtgt catagaagta tattgataaa atgcagctga atatttactt ggattagaat
     1501 aaccaatctc attagctatt tcgataattg gaatatcggt attaatcagt agacctaaac
     1561 tatactctaa acgatattct ttataccact gatagattgg tttaccgtga atctctttaa
     1621 atgctttacg aaatgtatga atattaatat
//
LOCUS       DAOMTA010000749         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3848_length_1649_cov_3.274153, whole genome shotgun sequence.
ACCESSION   DAOMTA010000749 DAOMTA010000000
VERSION     DAOMTA010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3848_length_1649_cov_3.274153"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1346
                     /locus_tag="PLK68_15230"
     CDS             471..1346
                     /locus_tag="PLK68_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRM91801.1"
                     /translation="MTNYLNEYKQMIVFRNLSDHTFKSYCTYITVYLDYCQNILYKSP
                     EDMSWQDIRDFIMFLQCKRNLANRTINACIAQLKFFTNYVLHKKWDDFQVPKKKFNTF
                     LPFVPSQQQVNDFIDSFDNLKHKAMVALMYSSGLRLSEVCALKYDDITRANMRIRIRY
                     AKNRQEDFVPLSKKTLDILTKYWKEFGKPTNWLFPKQRDKNNVFDKPIDTFYISRHIK
                     AKCESLKLPYKITCHSLRHAYGTHLYEMGNDILTIKKLLRHKSLASTLIYVHIANNSF
                     NNVINPFDEFEHGRS"
     gene            1346..>1649
                     /locus_tag="PLK68_15235"
     CDS             1346..>1649
                     /locus_tag="PLK68_15235"
                     /inference="COORDINATES: protein motif:HMM:NF025677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HRM91802.1"
                     /translation="MNTIQDVFNASFHEYKNKNFVSNVQSKAAFSIMNCKTNKMGFNK
                     STCQNCNHEEIHYNSCKNRNCPNCQIIQKDIWIDSRKAELVDTPYFHVVFTIPDELN"
BASE COUNT          539 a          292 c          224 g          594 t
ORIGIN      
        1 gcttgcggct gaacgcctat cgcagtttta cgttcgaagt ggctctagag tttctattct
       61 ataatttatt gtaccaaatc gctattctag cgatggtcag acacgtcaat agcgttcaat
      121 actatctctt aacgtcgtca tatttcttta aaaattttta acctctatat aaaaacttct
      181 ttaattataa taatatgttt tgcttcttta tccttttaat gttaaaataa ggttattggc
      241 aaaaaaaata gtgatggtgt ttgttttatg tgtagtactt acgtacataa tcaagatcca
      301 tcacaatcat gacaccccca acaaccccat cactattatg actatcttaa catattttat
      361 tttatttttc aagtacatat ttcatttttt tgttagacaa cctcctttga ttgtgttttt
      421 tacgaatgtt aacactttct taattttaaa aaagatcaaa ggagactttt atgactaatt
      481 atcttaacga atacaaacaa atgattgttt ttagaaactt atccgaccac acttttaaat
      541 cttattgcac ttatattact gtttatctcg attattgtca aaacattctt tataaatcac
      601 ctgaagatat gtcttggcaa gatatccgtg attttattat gtttttgcaa tgcaagcgca
      661 atctcgctaa tagaactatc aacgcttgca ttgctcaact aaaattcttt accaattacg
      721 tccttcataa aaaatgggac gattttcaag tacctaaaaa aaagttcaac acttttctac
      781 ctttcgtgcc atctcaacag caggtaaatg attttatcga ttcttttgat aacttgaaac
      841 ataaagctat ggtggctctt atgtattctt ccggtcttag acttagcgag gtttgtgctc
      901 tcaaatacga tgatatcact agagctaata tgagaattcg gattagatac gctaaaaata
      961 ggcaggagga tttcgttcct ttatctaaaa aaactttaga tatactcact aaatactgga
     1021 aagaatttgg caaacctacc aactggctct tccctaagca aagggataaa aataatgttt
     1081 ttgataaacc tattgatact ttctatatct ctagacatat taaagctaaa tgtgaatctc
     1141 ttaaacttcc atacaaaatt acttgtcatt ctcttagaca cgcttatggt actcaccttt
     1201 atgaaatggg taatgatatt ttaactatca aaaaacttct taggcataag tctttagctt
     1261 ctactcttat ttacgtacac attgctaata actcttttaa taatgtcatt aacccttttg
     1321 atgagtttga gcacgggagg agctaatgaa cactattcaa gatgttttta acgcttcttt
     1381 tcatgagtat aagaataaaa acttcgtttc taatgtgcaa agtaaagctg ctttttctat
     1441 tatgaattgc aaaactaata aaatgggatt taacaaatct acttgccaaa attgtaatca
     1501 cgaagaaatt cactacaact cttgcaaaaa tagaaattgc cctaattgcc aaattattca
     1561 aaaagatatt tggattgatt ctagaaaagc tgagcttgtt gacacgcctt attttcatgt
     1621 tgtgtttaca atccctgatg aactcaatc
//
LOCUS       DAOMTA010000750         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3890_length_1636_cov_1.881088, whole genome shotgun sequence.
ACCESSION   DAOMTA010000750 DAOMTA010000000
VERSION     DAOMTA010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3890_length_1636_cov_1.881088"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PLK68_15240"
     CDS             <1..165
                     /locus_tag="PLK68_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRM91803.1"
                     /translation="EVLDNNPQSIEDYSNGKDRAVGFLVGQIMKKTGGQANPGLTSKL
                     LIELLKQRIS"
     gene            215..958
                     /locus_tag="PLK68_15245"
     CDS             215..958
                     /locus_tag="PLK68_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91804.1"
                     /translation="MSQRKRFSFDDEVDDTIDLSKDDLKNSSITNNDKPSNDNVFADE
                     DGLDAKAYPTDNGGDKSMKNKKKRKFKIKKWQIALIAFLSIIIVFVIYVFVAGGNDGP
                     VYGDRCASLIAIDKSKFTGVEDAIKADPSVNSINIEVDCRIVKISMNFVDNTSSDTAK
                     QLATNALHTLDDTLGENKEEGRAYSNLFTTANGRGQYNVEFVLTSNGDTNFPIFGTKH
                     PNSDDITFTGANVVDQAATDRAANTNANQ"
     gene            996..>1636
                     /locus_tag="PLK68_15250"
     CDS             996..>1636
                     /locus_tag="PLK68_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM91805.1"
                     /translation="MKKNDYFYGECTDLTHDGQGVVKIDNFTYFVKGMLTGERGRLKV
                     IKVLKNYGIARLIELEVTSKYRIDPKCSVFRPCGGCQLQHLNPQGQMIYKTKRVKDCL
                     ERIGNCKVAVNDCIMMEKPWHYRNKVQMPVGVKGNHLVTGFYKQKSNEIIPCENCYIQ
                     NELSNQITNRVKELMEEFKIYPYDKLAHQGNIKHILTKHGYHTDEVMLVLISYR"
BASE COUNT          608 a          215 c          312 g          501 t
ORIGIN      
        1 gaagttcttg ataataatcc tcaatcaatt gaagattata gcaatggtaa agatcgagct
       61 gtgggattct tagttggaca aattatgaaa aaaaccggtg gtcaagctaa tcctggtctt
      121 acaagtaaat tactaattga actattgaaa caaagaattt cataatgtac aaataattct
      181 atatttggta caatggttga aggaggatat aaatatgtct caaagaaaaa gatttagttt
      241 tgatgatgaa gtagatgata caattgatct atcaaaagat gatcttaaaa attcatcaat
      301 aactaataat gataaacctt caaacgacaa tgtctttgct gatgaagatg gtttagatgc
      361 caaagcttat cctactgata acgggggaga taaatcaatg aaaaacaaga agaaacgcaa
      421 gtttaaaatt aagaaatggc aaattgcatt aattgctttt ctatcaataa ttattgtgtt
      481 cgtcatctat gtatttgttg ctggaggaaa tgatggacca gtttatggtg atcgttgtgc
      541 ttctttaatt gcaattgaca aaagtaaatt tacaggagtt gaagatgcaa tcaaagcaga
      601 cccaagcgtt aatagtatca atattgaagt agattgtcga attgttaaaa ttagtatgaa
      661 ttttgttgat aatacttctt cagatactgc taaacaactg gcaacaaacg ccttacacac
      721 attagatgat actttaggtg aaaataagga agaaggtaga gcatatagta atttatttac
      781 tactgctaat ggccgtggac aatacaacgt agagtttgtt ctaacgagca atggtgatac
      841 taatttccct atatttggta ctaaacatcc aaatagtgat gatattactt ttactggagc
      901 taatgtagtt gatcaagctg caactgatcg tgcggctaat acgaacgcta atcaatagac
      961 gagcatggct cgtctttatt tatgaggagg actaaatgaa aaaaaatgat tatttttatg
     1021 gtgaatgcac agatttaacc cacgatggtc aaggagttgt taagattgat aactttacat
     1081 actttgtaaa aggtatgctt acgggtgaaa gaggacggct aaaagtaata aaggtattaa
     1141 agaattacgg aattgcacgt ttaattgaat tagaagttac ttcaaaatat cgtattgatc
     1201 ccaaatgctc tgtctttaga ccatgcggag gctgtcagtt acaacatctt aatcctcaag
     1261 gacagatgat ttataaaaca aaacgggtca aagattgttt agaaagaatc gggaattgta
     1321 aagtggcggt aaatgattgt attatgatgg aaaaaccgtg gcattatcgc aataaagttc
     1381 aaatgccagt tggagttaaa ggaaatcatt tagtaactgg tttctataaa caaaagtcta
     1441 atgaaatcat cccatgtgaa aactgttata ttcaaaatga attatcaaat cagataacta
     1501 atcgagttaa agaattaatg gaagagttta aaatttaccc atatgataag ttagctcatc
     1561 aaggtaatat taagcatatt ttgactaaac acggttatca tacagacgaa gtaatgttag
     1621 tactaattag ttatcg
//
LOCUS       DAOMTA010000751         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3893_length_1635_cov_2.057595, whole genome shotgun sequence.
ACCESSION   DAOMTA010000751 DAOMTA010000000
VERSION     DAOMTA010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3893_length_1635_cov_2.057595"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLK68_15255"
     CDS             <1..397
                     /locus_tag="PLK68_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM91806.1"
                     /translation="STNEYSPFETNYIFQYGRPLDLELMREAAKIFIGEHDFASFCSY
                     DQYGNTIRELYELSITNEKGIIKFILVGNGFRRYMVRHIVGGLIQVGAKRVTKERLQE
                     LLDSKGEQKCLFKAKPQGLYLHEVFYDEN"
     gene            387..1262
                     /locus_tag="PLK68_15260"
     CDS             387..1262
                     /locus_tag="PLK68_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRM91807.1"
                     /translation="MKTNFHTHTFRCGHAVGNEEAMVKSAIEEGIEVLGFSEHVPLPR
                     YRKHIFKGMRYTLNHFRSFAVACKAIITNGPAMRMPYKDKQLHLEEVKRLKEKYCDQI
                     TIYQGFEAEYFEEYLDYYQGLLNSGEADYLILGNHFNKCTVHTRYYGKMDISDEEIIS
                     YKNDLLKALDTNLFSYVAHPDLFMVGKVYFDELCENITREICQKALEKDVPLEVNAGG
                     IRRGYRKVGDEIQYPYPNSHFFDIVGEVGCKVILGIDAHSPDDFNQDDFEALEKFAWR
                     HKLNVINIPEFKKGK"
BASE COUNT          566 a          184 c          335 g          550 t
ORIGIN      
        1 aagtacaaat gaatatagtc catttgaaac gaattatatt tttcaatatg gacgaccatt
       61 agatttagaa ttgatgagag aagcagctaa aatatttatt ggtgagcatg attttgcatc
      121 attttgtagc tatgatcagt atggtaatac aattagagaa ctatatgagt tgtcgattac
      181 taatgaaaag gggattatta aatttatttt agttggaaat ggatttagac gttatatggt
      241 tcggcatatc gttggtggac taattcaagt tggggcaaaa cgggttacta aagaacgatt
      301 acaagagtta ttagatagca aaggtgaaca gaagtgtttg tttaaggcta agccccaggg
      361 actatactta catgaggtgt tttatgatga aaactaattt tcatacacat acattccgct
      421 gcggtcatgc ggttggcaat gaagaagcaa tggttaaatc agcaattgaa gaaggtattg
      481 aagttttagg ctttagtgag catgttcctt taccacgtta tcgtaaacat atctttaaag
      541 gaatgcgtta tactcttaat cattttcgtt cgtttgcggt tgcttgtaaa gcaatcatta
      601 ctaatggtcc tgcaatgcga atgccatata aagataagca gttacatctt gaagaagtaa
      661 agcggctaaa ggaaaaatat tgtgatcaaa ttacaatata tcaaggtttt gaagctgagt
      721 attttgaaga atatttagat tattatcaag gattattaaa ttcaggagaa gcagattatt
      781 tgattttagg aaatcacttt aataagtgca ctgttcatac tcgttattat ggtaagatgg
      841 atattagtga tgaagaaata attagttata aaaacgattt gttgaaggca ttagatacta
      901 atttattttc ttatgtagct catcctgact tatttatggt tggaaaagta tatttcgacg
      961 agttatgtga aaatattacc cgggaaatat gtcaaaaagc attagaaaaa gacgttcctt
     1021 tagaagttaa tgctggtggg attcgccgtg gctatcgtaa agtcggggac gaaatccaat
     1081 atccatatcc caatagtcac ttttttgata ttgttggaga ggtagggtgt aaagtgattc
     1141 ttggaattga tgcgcatagt cctgatgatt tcaatcagga tgattttgaa gctttggaga
     1201 agtttgcatg gcgtcacaaa ttaaatgtaa ttaatatacc tgaatttaaa aaaggtaaat
     1261 aaggatggaa tctaagagcg ttaggttcca tcttttatca cgaataatta ataaaggagc
     1321 gacaatattt tagggtatta tagctaatga tttacaagca taaataagat attatatgct
     1381 gttgtttaag tatataatgg tagtattgaa atttttttgt aagatcaaaa gaagatcaag
     1441 aagtttcaat ctgaataaag attattgaag tgaatcatac ttttttatgg tcaatcaatt
     1501 gatggagtgg gagaactagt tactgcagtt aagacaggtg aggatattga ttatcaaaat
     1561 tatcttttat taacaatact tggtttacaa gatgtatcgg ttattatgaa aaaacatagc
     1621 taatatttaa taagt
//
LOCUS       DAOMTA010000752         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3900_length_1634_cov_1.576947, whole genome shotgun sequence.
ACCESSION   DAOMTA010000752 DAOMTA010000000
VERSION     DAOMTA010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3900_length_1634_cov_1.576947"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLK68_15265"
     CDS             <1..965
                     /locus_tag="PLK68_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRM91808.1"
                     /translation="FKEKREYLAAKLLTIFMEYPIIFMGYSISDSNIRDILSSIVKCM
                     SNEQVKLLKEKFIFIEYDAEMEGYEIGESSFAFEDDKMITMTKITLSDYSLIYNAIGK
                     KKMKIPVRLLRVLKDELYLFTLSNEPTKNIKVAPIDDDRVANDELVISIGTTETVNLN
                     GLKGISTDQWYRNIVMQDLKYDTKDLLAYAPTLAKQVSGILPINSLYEDKFKDIEGID
                     KLLVNDFEDIISKTIERERRRHPQVDIQSILKEITDIDKQMLNIAYLKKEQINLINLE
                     NYLKSKFEQDSEILKSSKQSTRTNLRRLIRIYDYLKGENKKASN"
BASE COUNT          642 a          186 c          264 g          542 t
ORIGIN      
        1 aatttaaaga gaaacgtgaa tatttggctg ctaaacttct tactatattt atggagtatc
       61 caattatttt tatgggatat tctatttcgg attctaatat cagagatatt ttaagttcaa
      121 tcgttaagtg catgtcaaac gaacaagtta aattactgaa agaaaaattt atttttattg
      181 aatatgatgc tgaaatggaa ggatatgaga taggtgaatc atcatttgct tttgaagatg
      241 ataaaatgat tacaatgaca aagattacct tatctgatta ttcattgatt tataacgcaa
      301 ttgggaaaaa gaaaatgaaa atacctgttc gtctactaag ggtactaaaa gatgaattat
      361 atttattcac attatctaat gaacctacga aaaatattaa agtagcacca attgatgatg
      421 atcgagttgc aaatgatgaa ttagtcattt ctattgggac aacagaaaca gttaatttaa
      481 atggtttaaa aggtatatct acagatcaat ggtatagaaa tattgtgatg caggatctta
      541 aatatgatac taaagaccta ttagcttatg ccccaacact agcaaaacaa gtttctggta
      601 tattacctat caattcatta tatgaggata aatttaaaga tatagaggga atcgataagt
      661 tgcttgtaaa tgattttgaa gatattattt ctaaaacaat agaaagagaa cgtcgtagac
      721 atcctcaagt tgatatacaa agtattttga aagaaataac tgatatagat aaacaaatgc
      781 ttaatattgc gtatttaaaa aaagaacaaa taaacttgat taatttagaa aattatttaa
      841 aaagtaagtt tgaacaggat agtgaaatat taaaatcatc taaacaatct actcgtacaa
      901 atttaagaag attgattaga atttatgatt atttaaaagg agaaaataaa aaagcctcta
      961 attaaagcga cctttccaag gacaccatta gaggtttctt tctcttcatg aaaacattcc
     1021 aacaaggcta ctttataaaa gccaccctat tttcctgctc tcttactaag tattatacac
     1081 ttttatcaga aaattacaat tgaatatcga aagattaaga aattatttgt tttcgagagt
     1141 atttttgtag acttctttta aagaagtggc tcattttaaa aaagtaattt aataaagtga
     1201 gccgaaaagt gagccgaaaa tagattgggc tcaaaacagc agaaaattta actttatgta
     1261 agtcgttaag ataaaaaatc tagcgacttt tttatttgca aacggaaagg aaaaagataa
     1321 tgagcatatt ttttatattg ggagtcggaa tcgtattcgg ctcttttttg tgttggttac
     1381 ttatcagtat ggtaatcaat gatgattgtg gtgttgtgtt aattgaatat gataccagta
     1441 aagaaagaga ggtcgaactt taatggataa agatattttt gaagatatgt ttcattggat
     1501 acaaagtgag tatttatcag acttacagtt caagaagaaa gaagtatttc aaaaattaaa
     1561 aacaatagat tatacaaagt attcatcgtc taacttaaaa gatttctttg aatatatttt
     1621 tcaaaaagaa atac
//
LOCUS       DAOMTA010000753         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3910_length_1632_cov_2.167406, whole genome shotgun sequence.
ACCESSION   DAOMTA010000753 DAOMTA010000000
VERSION     DAOMTA010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_1632_cov_2.167406"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..590
                     /locus_tag="PLK68_15270"
     CDS             135..590
                     /locus_tag="PLK68_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91809.1"
                     /translation="MLLVFPLYADGIPATLLNFLKSLENNLPDKKTVISVIINCGFLE
                     YTQNDLAVKMIELFCKKTNCEFGSVLKIGSGEAILKTPFKIFVTRKVKKLANSILSKN
                     YKTLHITMPLTPKLFIKASTKYWIEYGNRNGITKEQMDTMSIEDDNTLV"
     gene            complement(815..967)
                     /locus_tag="PLK68_15275"
     CDS             complement(815..967)
                     /locus_tag="PLK68_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91810.1"
                     /translation="MHKAIKKNITEYNDGIYNFIVDLIDENNDYFVQKVQIDAKDDAP
                     RFSNKD"
BASE COUNT          610 a          232 c          190 g          600 t
ORIGIN      
        1 cactcgtttt ggtttgacga cgaaaactct gtccagacat tttttcaaaa aaatataata
       61 aagacattga atattacaat ataacaaagc aaaatcatga taaattatgt tctaaaatga
      121 atgatttctc cgatatgtta ctggtttttc cattatatgc tgacgggatt cctgctacac
      181 ttttaaattt tcttaaaagt cttgaaaaca acttaccaga taaaaaaaca gtaatttcag
      241 taataataaa ttgtggattt ttagaatata cacaaaatga tttagcagtt aaaatgatag
      301 agctgttttg taagaaaaca aattgtgagt ttggatctgt tttaaaaatt ggtagtggag
      361 aagcaatcct aaaaacccct ttcaaaatat ttgtaacaag aaaagtaaag aaattagcaa
      421 attcaatcct atcaaaaaac tataaaacac tccatataac aatgccgctc accccaaaat
      481 tatttattaa agcatcaaca aaatattgga ttgaatatgg aaacagaaat ggtatcacaa
      541 aagaacaaat ggatacaatg agtatagagg atgacaatac cttagtttaa cagctatatc
      601 aaaaaggagc tcttatgatt ttaaatgtag ttagttttga tgaagctgcg ctccatgttc
      661 ttttatacta gcaattattc atatattgtg tttttggata gaagtttaga gtgtttatcg
      721 agagcccaac tagattatat tttacatcaa gtccaaacag cccaactaat ttcaatatct
      781 tctgttttag aattttagtc ccattttcta ttagctaatc tttatttgaa aatctcggag
      841 catcatcctt agcatctatt tgaacttttt gtacaaaata atcattattt tcatctatta
      901 aatcaacaat aaaattatat attccatcat tatactcagt aatattcttt ttaatagctt
      961 tatgcattaa agaaaagaac tcaatatagc ttatatttgc agtaagtatt aatcctatac
     1021 aatcctctat attgtttttt tgtattatgt atgcctacaa aactcatatt gctacaatcc
     1081 ttttttaata aataatcagc tgcatacatt atattaccgc caattgtatt ctatcaaaat
     1141 atgtatttat ttactgcttt tagaatatta aaacaaaaaa tctctttagt tttgtttatc
     1201 taaagagaaa ttttatatta taaaaatgtt agcaaatttt aaagatgctt ttgcagcaga
     1261 tataacaatc ataatatttt ttctttacct gtaagctaga ttaatataat atatggaatg
     1321 ataactacta ttttatacta ttcagttcta tcttagtatt ccatatcgat gatagtcaaa
     1381 ttagtctgaa tctgatgatt tcaaacaatg tcaaactgca acatttttaa aattgtcata
     1441 tgcattaaat tatatatcat ttattatcat acctaaagac attcttcgtc aaaatgataa
     1501 atccttaatt gattttacac aactttttga tatcatgcat taaataatcc ttctctatta
     1561 gaggcttgaa tatatttaaa atattatcat ctaatttatt caaaatctga atatagataa
     1621 ttggatgata gt
//
LOCUS       DAOMTA010000754         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3912_length_1631_cov_2.432741, whole genome shotgun sequence.
ACCESSION   DAOMTA010000754 DAOMTA010000000
VERSION     DAOMTA010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_1631_cov_2.432741"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1418)
                     /locus_tag="PLK68_15280"
     CDS             complement(30..1418)
                     /locus_tag="PLK68_15280"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM91811.1"
                     /translation="MSRFPDNFLWGGASADFQYEGGFGEGNRGLITADFVTDGNVDTL
                     RQVTYKCKDGTLGSSPLKAEIPEGATGYIDPERYYPSHDGVDFYHRYKDIALMAEMGF
                     NVYRFSICWTRIFPTGEEAEGNELGLKFYENVIDELLKYHIEPLITICHDEIPAYLAD
                     HYDGWSSRHTINCYLKLCKTLFERFKGKVKYWLTFNELNVVKGYAQMGTHKIDSQTHY
                     QAMHHVFVASSLATKMAHEMMPGCMVGTMYAMSGLYPLTCKPEDMMAHMNTRRLSYFY
                     ADTMVTGGYPYYAQALFEKEGVVLVKEPGDDDLLKEYPLDFITFSYYRTTTVNANTKL
                     NIIGLAMDLNPYLEATPWGWPIDPVGLRYVMNELYDRYKKPIMIVENGMGEIDHFEND
                     TVIDDYRIRYLKDHFKNMKDAINIDHVDCIGYTMWGAIDLVSLSTGEMKKRYGFVYVD
                     KNDDGSGSDNHL"
BASE COUNT          547 a          334 c          209 g          541 t
ORIGIN      
        1 tgaaatcgat cattttgaaa atgatacagt tatagatgat tatcggatcc gctaccatca
       61 tcatttttat ctacataaac aaaaccataa cgttttttca tttccccggt actaagtgaa
      121 acaagatcaa tagcccccca catcgtataa ccaatacaat ctacatgatc aatattaatg
      181 gcatctttca tgtttttaaa atgatctttt aaatatcgga tccgataatc atctataact
      241 gtatcatttt caaaatgatc gatttcaccc attccgtttt caacaatcat aattggtttt
      301 ttataacgat cataaagttc attcataaca tagcgcaatc caacaggatc aattggccaa
      361 ccccaagggg ttgcttctaa atagggattt agatccattg ctaatccgat aatatttaat
      421 ttagtgttag cattaacggt tgttgttcta tagtagctaa aagtaataaa atctagtgga
      481 tattctttta gtaaatcatc atcacctggc tccttaacca gtacgacccc ttctttttca
      541 aataatgctt gtgcataata tggatatccg ccagttacca tagtgtctgc ataaaaatat
      601 gataatcttc tagtattcat atgtgccatc atgtcctcag gcttgcaagt taatggatat
      661 aaaccactca ttgcatacat tgttcctacc atgcaccctg gcatcatttc atgagccatt
      721 tttgttgcca atgaactagc aacaaaaaca tgatgcatcg cttgataatg cgtttgacta
      781 tcaattttat gagttcccat ttgagcataa ccctttacaa cattaagctc attaaatgtc
      841 aaccaatatt ttactttccc tttaaaacgt tcaaataatg ttttacataa ttttaagtag
      901 caattaattg tatggcgtga tgaccatcca tcataatgat cagctaaata tgcaggaatt
      961 tcatcatgac aaattgtaat taaaggctct atgtgatact ttaataattc atcaataaca
     1021 ttctcataaa acttcaaacc taactcattt ccttcagcct cttcgcctgt cggaaaaatt
     1081 cttgtccagc aaatactaaa acgataaaca ttaaatccca tttctgccat taaagcgata
     1141 tctttataac gatgataaaa atcaactcca tcatgacttg gataataacg ctcaggatca
     1201 atatatccag ttgctccttc aggaatttca gcctttaatg gtgacgaacc aagcgttcca
     1261 tctttacact tataagttac ctgacgtaac gtatcaacat taccatctgt tacaaaatct
     1321 gctgtaatca agccgcgatt accttcacca aatccaccct catattgaaa atcagcacta
     1381 gctccacccc ataaaaaatt atctggaaat ctactcatac tttgttcctc ctttgatact
     1441 actattataa cggagttttt tatataaact taaaagttcg cattttggaa acttctatat
     1501 tcagcaacaa tcaagtcatt aatcaaagga tagatataac tatgtgtttg taatttagat
     1561 aaatgctgat agacaagaca aatatcaaca tcgggatgat ttaccatcat ttcgtttgca
     1621 gtaattaaaa t
//
LOCUS       DAOMTA010000755         1629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3917_length_1629_cov_2.346252, whole genome shotgun sequence.
ACCESSION   DAOMTA010000755 DAOMTA010000000
VERSION     DAOMTA010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_1629_cov_2.346252"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /locus_tag="PLK68_15285"
     CDS             <1..>1629
                     /locus_tag="PLK68_15285"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRM91812.1"
                     /translation="ETVSIQSSNKFDEKAYLALRNSLNIAYDSFIKKHNYINSRAIKN
                     LLERDPRQYLILNLESKGVEANTYIKSDIFKIRTINPVVEIKHVESIQDAISASLNFK
                     GMLDLNYMSIIYDKSIESIEKEMHSSAMVFYDPKEERWQLESEYLSGNILEKIDFIRE
                     NNLIDRYEKNINALNNVMPEPLTITDITFQLGAPYIPTHIYEDFACYLFRFENMMYDS
                     KSLTISFINETGQFAMDYYEYRFFGNSFIEDEWGVPLSENIDTDGVIKRQPRYNGFDL
                     LNDVLNSRIPTLKNYWEEINEGGIVKTKSEINSERTGQARELSSQLENTFVEFVFDNE
                     KYCREIEDEYNRLFNVIRPRIYNGEFLSFPEMNNSFQMEEYQKNAIARIIYGKNTLLS
                     QQVGAGKTFEMIASGMEMKRMGKRNKLLYVVPNHLIGQWRNEFMTLYPQANILAISTN
                     DFGTKKRADIVNKMAVNNYDAVIMGHSSFKLIPMDNEIQLNFLNEEIDKLVTAIEEAK
                     ANGSHQNTAVVKLLERSKKAVEKNVKKLTDIKRD"
BASE COUNT          634 a          201 c          311 g          483 t
ORIGIN      
        1 agaaacagtt agtatacagt catctaacaa attcgatgag aaggcatatc ttgctttaag
       61 aaattcatta aatatagcat atgacagttt tataaaaaag cataattata tcaattccag
      121 ggctattaag aatcttttag aaagagatcc acggcagtat cttattttaa atctggaatc
      181 taaaggcgta gaggcaaata catatataaa atcagatatt tttaaaatca gaactatcaa
      241 tcccgttgtt gaaataaaac atgttgaaag tatacaggat gcaatttctg catcattaaa
      301 ttttaaaggt atgttagatt taaattatat gagcattatt tatgataaaa gtattgaaag
      361 cattgaaaag gaaatgcact catcggctat ggtattttat gatcctaagg aagaaaggtg
      421 gcaattagaa agtgagtatc tttctggaaa tattctagaa aaaatagact ttataagaga
      481 aaataattta attgacagat atgaaaaaaa tatcaatgca ttgaataacg ttatgcctga
      541 accgttgact attactgata taacttttca attgggtgcg ccatatatac caacacacat
      601 atatgaggat ttcgcctgct acctttttcg atttgaaaat atgatgtatg attccaagtc
      661 actgacaata agctttatta atgaaacagg acagtttgca atggattatt acgagtatag
      721 attctttggg aatagtttta ttgaagatga atggggagta cctttatctg aaaatattga
      781 tactgacggt gtaattaaaa gacagccaag atataatggc tttgacctgc tgaacgatgt
      841 tttgaattcc agaatcccaa cgttgaaaaa ctattgggag gaaataaatg aaggtggcat
      901 cgtaaaaaca aaaagtgaaa ttaacagcga aaggactgga caggcaagag aattgtcgtc
      961 acagttggaa aatacatttg ttgagttcgt atttgacaat gagaaatact gcagagagat
     1021 tgaagatgag tataatcgac tgtttaatgt tataaggcca agaatttata atggagaatt
     1081 tttaagcttt cctgaaatga ataacagttt tcagatggaa gaatatcaaa aaaatgctat
     1141 tgcaagaata atctatggca aaaatactct tttatcacaa caggtgggag ctggcaaaac
     1201 attcgaaatg attgcaagtg gtatggaaat gaaaagaatg ggtaaaagaa ataaactgct
     1261 gtatgttgta cctaaccatt tgattggaca atggcgtaat gaatttatga cactatatcc
     1321 acaggcaaat attttggcaa tttctactaa tgattttggc acaaagaaaa gagcagatat
     1381 agtaaataag atggcagtga ataattatga tgcagtaata atgggtcact cgtcttttaa
     1441 actgattcct atggataatg agatacagtt aaattttttg aatgaagaaa tagataaatt
     1501 agtaactgca attgaagaag caaaagcaaa tggaagccat caaaatactg ctgttgttaa
     1561 gttgctcgaa cgttctaaaa aggccgtaga aaaaaatgta aagaagctta cagatattaa
     1621 aagagatca
//
LOCUS       DAOMTA010000756         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3922_length_1628_cov_2.251113, whole genome shotgun sequence.
ACCESSION   DAOMTA010000756 DAOMTA010000000
VERSION     DAOMTA010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_1628_cov_2.251113"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLK68_15290"
     CDS             complement(<1..1467)
                     /locus_tag="PLK68_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM91813.1"
                     /translation="MLIECSNITKSFQGIPLLKDITFKIDDHDKVAIIGVNGAGKTTI
                     LKIIAGEENYDSGDLFCNKELTLGYLKQQHDLAMDKTVYDVGLDVFAPLITIENRLRQ
                     LENEMTTDHSERILNEYDRLTQSFHDKDGYSYPSKLTGVLKGLGFSEEEFNLKVSMLS
                     GGQKTRLALAKMLLQEPKLLLLDEPTNHLDNSAINFLEGYLKNYPHAIITVSHDRYFI
                     DQVSNKIVEVENGKSKSYKCKYNEYSILKKKQRAVDLKHYLNQQKEIKRIQESIDTLK
                     SYNREKQVKRAESKEKQLAKIERIDKPENLPDTITINFKPKRESGFDVLKIENLAVKF
                     DEILFKNIDIDIKKQERIALVGDNGVGKTTLFKTILDQLNAYQGKIKFGSKVDLAYYD
                     QEHSTLAMDKTIFNEISDNFPKMTNTEIRNSLALFNFKGDDVFKEISLLSGGEKGRVV
                     LTEILLKQANLLILDEPTNHLDIASKEVLEDALNQFEGT"
BASE COUNT          512 a          252 c          231 g          633 t
ORIGIN      
        1 tgttccttca aattggttta aggcatcttc aagaacttct ttagaagcaa tgtctaagtg
       61 gttggttggt tcgtctaaaa ttaaaagatt tgcttgtttt aataatattt cagtaagtac
      121 aacacgtcct ttttcaccac cagaaagtaa agagatttct ttaaaaacat catcgccttt
      181 gaagttaaat aaggcaagac tgttgcgaat ctcagtatta gtcatcttag gaaaattatc
      241 actaatttca ttaaaaatag ttttatccat tgctagtgtg ctatgttctt gatcatagta
      301 agcaaggtct actttactac caaatttaat tttaccttga taagcattta gctgatcgag
      361 gatagtttta aaaagagttg ttttacccac accgttatcc ccgacaagag caatacgctc
      421 ttgttttttt atatcgatat caatgttttt aaataaaatt tcatcaaatt taacagctag
      481 gttctctatt tttaaaacat caaaaccgga ctcacgtttt ggtttaaaat taatcgtgat
      541 tgtatctggc aagttctcag gcttgtcaat acgttcaatt ttagctaact gcttttcttt
      601 agattcggct cgtttaactt gtttttcacg gttatatgat tttagtgtat cgatactctc
      661 ttgaattctt ttaatttctt tttgttgatt taaataatgt tttaaatcaa cagctctttg
      721 ctttttcttt aaaattgaat attcgttata tttacattta tagcttttgg attttccatt
      781 ttcgacttct acaattttat tagaaacttg atcaataaag taacgatcat ggctgactgt
      841 gatgattgca tgaggataat tttttaaata tccttctaag aaattaatag cactattatc
      901 taaatggtta gtaggttcgt caagtaacaa tagctttggt tcttgaagca gcattttagc
      961 aagagctaaa cgtgtttttt gaccaccaga tagcattgaa acttttaaat tgaattcttc
     1021 ttcgctaaag cccagtccct ttaaaacgcc ggttaattta ctgggataac tatatccatc
     1081 tttatcatga aatgattgag ttagacgatc atattcattt aaaatacgtt cactatgatc
     1141 agttgtcatt tcattctcta attgacgtag tcgattttca attgtgatca atggtgcaaa
     1201 aacatcaagg ccaacatcgt aaactgtttt atccatcgct aagtcatgct gttgttttaa
     1261 ataacctagt gttaattctt tattacaaaa aaggtcaccg ctatcatagt tttcttcgcc
     1321 cgcaataatt tttagaatag tagttttacc agcaccatta actccgataa ttgcgacttt
     1381 atcatgatca tcaattttaa aggttatatc ttttaataag ggaatccctt gaaatgattt
     1441 tgttatattt gaacattcta ttaacatatt ttatcaccca atgtattcta acatattttc
     1501 attgatattt atagtaaggc ttttgtttta agaagaaaag tagatatttt attttaggaa
     1561 ttcacaaatt tattattaaa aaagtgtaat taaaaattac tcgattaaat caagtaaata
     1621 atagattt
//
LOCUS       DAOMTA010000757         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3924_length_1627_cov_1.938931, whole genome shotgun sequence.
ACCESSION   DAOMTA010000757 DAOMTA010000000
VERSION     DAOMTA010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_1627_cov_1.938931"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..>395
                     /locus_tag="PLK68_15295"
     CDS             86..>395
                     /locus_tag="PLK68_15295"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRM91814.1"
                     /translation="MSGLEFNLKQYDKSNSEDLMREFDKRKKVEPIAIEDIKYNPLNP
                     DFDTLEDIVEFADKIYHNPNGVLEVITVYRDSDGKYMLVSGHKRIKACKYNIEHESEL
                     D"
     assembly_gap    396..437
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <438..1043
                     /locus_tag="PLK68_15300"
     CDS             <438..1043
                     /locus_tag="PLK68_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91815.1"
                     /translation="KPDNEIEEMNTILTYNDYRRFDTEEKKYTLFKAYYQYVAVLNKN
                     GEFKGRIREFISKQSGLGLKKVGDFIDELENTVLMYLNEFKDDVNRNLVNIEERNDYL
                     IHKSGLPIETLQLVLTKIKKKIEEDPKVKNDDEVLTGQTSIDDFVKDDLSIHYEEVRM
                     KLTNYFDAKCTVTKNNKITINCVDIKDLNRVLQMMGVITND"
     gene            1374..>1627
                     /locus_tag="PLK68_15305"
     CDS             1374..>1627
                     /locus_tag="PLK68_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91816.1"
                     /translation="MLSSGNYIEYKTIKDTAIIKSFIKTKAYSNQANIESKIDKLIAY
                     EKKRNATKKNYCIKLRVMISRSLSKQQKHELAKQFMFNISL"
BASE COUNT          670 a          159 c          286 g          470 t
ORIGIN      
        1 tattttacag aacattataa gaaaactgat gtattcattt aatgaaaccg ttgtatataa
       61 aattaaaaga gggcgaggag atagtatgag cggattagaa tttaatttaa aacaatatga
      121 taaatctaac agtgaggatc tgatgaggga atttgataaa agaaaaaagg tagaaccgat
      181 tgcaatagag gatattaaat ataatccatt aaatccagat ttcgatacac tggaggatat
      241 tgtagagttt gctgataaaa tttatcacaa tccaaatgga gtcttagaag ttattacagt
      301 ttatcgtgat agtgatggta agtatatgtt ggtaagcggt cataaaagaa ttaaagcctg
      361 caaatataat attgaacatg agagtgaatt agatgnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnaaa ccagataatg aaattgaaga gatgaatact attcttactt
      481 acaatgatta tcgtagattt gatactgaag aaaagaaata tactttgttt aaagcatatt
      541 accagtatgt tgcagtactc aataaaaacg gagaatttaa aggaagaatt agagaattca
      601 tttcaaaaca gagtggatta ggattaaaaa aggtaggaga ttttattgat gaacttgaaa
      661 atactgtact aatgtattta aacgaattta aggatgatgt gaacagaaat cttgtaaata
      721 tagaagagag aaatgattat cttatccaca aatcaggatt accaattgag acgctgcagt
      781 tagtgttgac aaaaataaag aaaaaaatcg aggaagatcc taaggtaaaa aatgatgatg
      841 aagtactgac aggacagaca tctatcgatg attttgtgaa agatgatcta agtattcatt
      901 acgaagaagt ccgtatgaaa ttaacaaatt attttgatgc gaagtgtacg gtaacaaaaa
      961 ataataagat tactattaat tgcgtggata taaaagattt aaacagagtg ttgcaaatga
     1021 tgggtgtgat tacaaatgat taatatgttt gaagtatata agtttgagag tttatcaaaa
     1081 gacgataaac tcactgttat cttaatcata ctgattggaa tattaatttt tataatattg
     1141 tgttggctga tgtttttgta cataagcgca ttgaaaggca aggcgaaaaa attaggcatt
     1201 aatcataaag agcagatcac aaaagtaata agaaaaatta aaaaacaaca ttaaattgag
     1261 tattataaaa aaattaaaat ttaactgaat aaatgataat tttttagatt tgacaaaaaa
     1321 ataaaaacat caaagtccct ttgatgttga tttagagagg agatatgaga cgcatgctca
     1381 gttctggaaa ctacattgag tataaaacaa taaaagatac agccattatt aaatctttta
     1441 tcaaaactaa ggcttattca aatcaagcaa atatagaatc taaaattgac aagctaattg
     1501 catatgagaa aaaaagaaat gcaactaaaa agaattactg tattaagtta agagtgatga
     1561 tttccagatc attgtctaaa cagcaaaaac atgaacttgc gaaacagttt atgtttaata
     1621 taagtct
//
LOCUS       DAOMTA010000758         1625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3931_length_1625_cov_2.287898, whole genome shotgun sequence.
ACCESSION   DAOMTA010000758 DAOMTA010000000
VERSION     DAOMTA010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3931_length_1625_cov_2.287898"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLK68_15310"
     CDS             <1..323
                     /locus_tag="PLK68_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM91817.1"
                     /translation="VHRIKVHALRHSHASLLISMGENPLIIKERLGHEKIQTTLGTYG
                     HLYPNSNYEVATKLTGIMKYTPAVESVVNATHNQFTADYHVEVEKNNAIRNAIKTEKS
                     RKTP"
     gene            complement(350..>1625)
                     /gene="guaA"
                     /locus_tag="PLK68_15315"
     CDS             complement(350..>1625)
                     /gene="guaA"
                     /locus_tag="PLK68_15315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRM91818.1"
                     /translation="LGGKVEKADVREYGKSHLIVSKQESKLMKDVAVESICWMSHFDY
                     ISKIAPGFEITSYTKDCPVASCEDEDKKLYAIQFHPEVLHTEYGTKMLSNFVLDVCNC
                     SGDWRMDSFVEEQIKAIREKVGNGKVLCALSGGVDSSVAAVLLSKAIGNQLTCVFVDH
                     GLLRKNEGDEVEAVFGPDGQYDLNFIRVNAQERYYEKLKGVEEPEAKRKIIGEEFIRV
                     FEEEAKKIGTVDFLVQGTIYPDVVESGLGGESAVIKSHHNVGGLPDAVDFKEIIEPLR
                     DLFKDEVRKVGLELGIPEYLVFRQPFPGPGLGIRIIGEVTAEKVRIVQDADAIYREEI
                     AKAGLDRSIGQYFAALTNMRSVGVMGDERTYDHAIALRAVNTIDFMTAEAAQIPYEVL
                     NKVMSRIINEVRGVNRVMYDITSKPPGTIEFE"
BASE COUNT          529 a          366 c          227 g          503 t
ORIGIN      
        1 gtgtccaccg tataaaagtc catgcactta gacactccca cgcttctctt ttaatcagta
       61 tgggagaaaa ccctttaatc ataaaggagc gtctgggaca cgaaaagata cagaccacac
      121 taggtactta tggacatctg tatcctaaca gtaattatga ggtggcaact aaattaactg
      181 gaattatgaa atacacccca gcagttgaaa gtgttgtgaa tgcaactcac aatcagttca
      241 ctgctgacta tcatgttgaa gtcgagaaaa ataatgcaat ccgtaatgca ataaaaacag
      301 aaaagagccg taaaaccccg taaaatgggg ctttacagct caccgcaagc tattcaaact
      361 caatcgttcc tggtggtttt gaagtaatat catacattac tctatttacc ccacgaactt
      421 cattaataat tctagacata accttattta atacttcata aggaatttga gcagcttctg
      481 ctgtcatgaa atcaattgtg ttaacagctc taagcgcgat agcatgatca taagttcttt
      541 catcacccat aacccccact gaacgcatat ttgtaagagc agcgaagtat tgaccgattg
      601 aacgatctaa tcctgctttt gcaatctctt ctctgtaaat tgcatcagca tcttgaacga
      661 ttctaacttt ttctgctgtt acttcaccaa tgatccgaat accaagtcca ggccctggga
      721 acggttgtct aaatactaag tattccggga ttcctaattc aagacctact ttacgaactt
      781 catctttgaa aagatcacgt aatggttcaa tgatttcttt gaaatccact gcatcaggta
      841 agcccccaac attatgatga gatttgatta ctgctgattc accacctaat ccactttcaa
      901 caacatcagg ataaatagtt ccctgaacta agaaatcaac agtcccaatc tttttagctt
      961 cttcttcaaa aacacgaata aattcttcac cgataatttt acgtttggcc tcaggttctt
     1021 caacaccctt taacttttca taatatcttt cttgagcatt aactcgaata aagtttaagt
     1081 catattgacc atcaggtcca aataccgctt ctacttcatc accttcattt ttacgaagta
     1141 atccatgatc aacaaaaaca caagttaatt ggtttcctat tgctttagat aataataccg
     1201 ctgccactga tgaatcaact ccaccagaca aagcacataa tactttacca ttaccaactt
     1261 tttctctaat agctttaatt tgttcttcaa caaacgaatc catacgccaa tcaccactac
     1321 aattacatac atctaaaaca aagttagata acatttttgt tccgtattca gtatgtaaaa
     1381 cttcgggatg gaattggatt gcatataact tcttatcttc atcttcacat gaagcaactg
     1441 gacagtcttt agtgtaagat gtaatttcaa agcctggagc aatttttgag atataatcaa
     1501 aatgactcat ccagcaaatc gactctaccg ccacatcttt cattaattta gattcttgtt
     1561 tactaacaat taaatgagat ttaccatatt ctctaacatc agctttttct actttaccac
     1621 ctaac
//
LOCUS       DAOMTA010000759         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3941_length_1623_cov_2.385842, whole genome shotgun sequence.
ACCESSION   DAOMTA010000759 DAOMTA010000000
VERSION     DAOMTA010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3941_length_1623_cov_2.385842"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLK68_15320"
     CDS             complement(<1..698)
                     /locus_tag="PLK68_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010046.1"
                     /note="catalyzes the fromation of N-acetyl-D-glucosamine
                     and N-acetyl-D-glucosamine-6-phosphate from
                     diacetylchitobiose-6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM91819.1"
                     /translation="MKGVKIVTIGGGSSYTPELVEGFIKRYAELPVKELWLVDIEEGK
                     EKLEIVGNLAKRMIKKAGLDMEVHLTLNRREALKDADFVTTQFRVGQLDAREKDELIP
                     LKYGVIGQETNGPGGMFKALRTIPVIFEIIKDCEELCPNAWIISFTNPSGINAEAVFR
                     HTGWKKFIGLCNGPVNMIKDIEKILKVKEEDHLDVKIGGINHMIYALDIQLNGKNANR
                     NLIDTMINDGNKES"
     gene            complement(685..1434)
                     /gene="nagB"
                     /locus_tag="PLK68_15325"
     CDS             complement(685..1434)
                     /gene="nagB"
                     /locus_tag="PLK68_15325"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRM91820.1"
                     /translation="MQVYVFKTEQDVDTYVGQQISTFIQDNDAPVIGFATGSTPLGAY
                     DYLIDSYQSGKTDFSKVRAFNLDEYVGIEKDHPQSFARAMKDYLFSKINIKEENIYSL
                     NGNAKDMTKECKEYDQLIINNPIDIQILGLGMDGHIAYNEPGSSFDSESHVVDLHPES
                     IQSSLDYGFTKIEDVPTQGVTQGIKTIMKARQLIMIAKGNKKAKLVERMLYGPVSEDF
                     PSSIIQTHNNVIVVLDQCAAANLKEETYERR"
     gene            complement(1444..>1623)
                     /locus_tag="PLK68_15330"
     CDS             complement(1444..>1623)
                     /locus_tag="PLK68_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM91821.1"
                     /translation="IEIPWTTPVFISGWIAGGWNALILQIVNFAVATAIYFPFVKVLD
                     RDLLKNAKKKELLQE"
BASE COUNT          498 a          324 c          196 g          605 t
ORIGIN      
        1 aaagattctt tatttccatc atttatcatc gtatcaatca aattacgatt agcattttta
       61 ccgtttaatt gaatatctaa tgcatagatc atatgattaa ttccaccaat tttaacatct
      121 aaatggtctt cttctttaac ttttaaaatt ttttcaatgt ctttgatcat atttacaggt
      181 ccattacata acccaataaa ttttttccag ccagtatgtc taaacactgc ttcagcattg
      241 attccagaag gatttgtaaa agaaatgatc caagcatttg gacacaattc ttcgcagtct
      301 ttaataattt caaaaataac cggaatagtt cttaatgctt tgaacattcc acctggacca
      361 tttgtctctt gaccaatcac tccatatttt aggggaatca actcatcttt ttctctagca
      421 tctaattgtc cgactctaaa ctgagttgtt acaaaatcag catcttttag tgcttcacga
      481 cgatttaaag tcaaatgcac ttccatatca agtccagctt tttttatcat tcgtttagcc
      541 agattaccaa caatttctaa tttttctttt ccctcttcaa tatctactaa ccataattct
      601 ttaactggta attctgcata tcttttaata aatccttcta ctaattctgg agtataactg
      661 ctgcctcctc caattgtaac gattttaacg cctttcataa gtttcctcct tcaaattggc
      721 tgctgcacat tgatctaata ctacaattac attattgtgt gtttgaatta tagagctagg
      781 aaagtcttca ctaactggac catataacat tctttccact aatttagctt ttttatttcc
      841 tttggcaatc ataattagtt gtcttgcctt cataatcgtt ttgattcctt gcgtaactcc
      901 ttgcgttgga acatcttcaa tttttgtaaa accataatcc aatgaacttt gaattgactc
      961 ggggtgtaaa tcaacaacat gactttcact atcaaatgaa ctacctggtt cattataagc
     1021 aatatgacca tccattccta gtcccaatat ttgaatatcg ataggattat taatgatcaa
     1081 ttgatcgtat tctttacatt cttttgtcat atccttagca tttccattta aagaataaat
     1141 attttcttct ttaatattaa ttttactgaa aagataatct ttcattgctc ttgcaaagct
     1201 ttgaggatga tctttttcaa tcccaacata ttcatcaaga ttaaaggctc taacttttga
     1261 aaaatctgtt tttccacttt ggtatgaatc aattaaatag tcgtatgctc ctaatggtgt
     1321 agaccccgtg gcaaaaccaa tcactggagc atcattatct tgaataaatg tactaatttg
     1381 ctgtccaaca tatgtatcaa catcttgttc ggttttaaat acataaactt gcataaagtc
     1441 ctcctattct tgtagcaatt cttttttctt agcatttttt aataaatcac gatctaacac
     1501 ttttacaaat gggaaataaa tagcagtagc aacagcaaaa ttgacaattt gaagaatcaa
     1561 tgcgttccat ccccctgcta tccagccaga aataaatact ggtgttgtcc aaggaatctc
     1621 aat
//
LOCUS       DAOMTA010000760         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3948_length_1621_cov_2.286718, whole genome shotgun sequence.
ACCESSION   DAOMTA010000760 DAOMTA010000000
VERSION     DAOMTA010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3948_length_1621_cov_2.286718"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLK68_15335"
     CDS             complement(<1..390)
                     /locus_tag="PLK68_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM91822.1"
                     /translation="MYLQLENVEKYYGENENRVQVLKGVNGSIERGEICVMLGPSGSG
                     KSTLLNLIGGIEIIDYGNVIVGGIDLASLSKTKLGLYRRDTLGFVFQFYHLVPNLTVK
                     ENIETGAYLSKTPLAVETLLDKLGLKEH"
     gene            complement(463..630)
                     /locus_tag="PLK68_15340"
     CDS             complement(463..630)
                     /locus_tag="PLK68_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91823.1"
                     /translation="MKNENYPYCVMMEINGMSCTDCQQQIENEFNQKKIYLEQHLAVL
                     LPKQDASVESV"
BASE COUNT          608 a          272 c          187 g          554 t
ORIGIN      
        1 atgttctttt aatcctaatt tatctaatag tgtttcaaca gctaatggtg tcttactcaa
       61 atatgcacct gtttcaatat tttcttttac tgttaaattt gggactagat gataaaattg
      121 gaaaacaaag cccaaagtat cccgccggta taaccctaat tttgtttttg ataaagatgc
      181 taaatcaatt ccaccaacta tcacattacc ataatcaatt atttcgatac ctccaattaa
      241 atttaataat gttgatttac cagagcctga aggtcctagc ataacacaga tttcaccgcg
      301 ttctattgaa ccattgaccc cttttaaaac ttgaacccga ttttcatttt caccataata
      361 tttttctaca ttttctaatt gcaaatacat ataaatcccc ctatctttta attcagattt
      421 ttttaattta ataacacgat agccaactaa ctttattaaa acctagactg attccacact
      481 tgcatcttgt tttggtagaa gtacagccaa atgctgttct aaataaattt tcttctgatt
      541 aaactcattt tcaatttgct gctgacaatc ggtacaactc atcccattaa tttccatcat
      601 cacacaatat ggataattct catttttcaa tcttacaaat tgatttttcg aatagaatca
      661 tccgattcac cacacaacac cctctataat ctttttaaaa taggatttaa tgctaaatat
      721 ttctattaaa acaattatat cttatctcct ggtgttagac ttgtcaaaca aatatataat
      781 aaaaaaataa actattacta gcatagttat tatttttagg caaagtttaa tatatcacat
      841 tgcaatacag tttttaagct tactgtctac cagatagata atcagtaaaa agtatcatag
      901 aagcttcatt tttagataaa ttaaatcagc aagatatttt gaatgaatat ttttagtaaa
      961 aggatatcca tgagtattac aaagataccg aactttttta taaagtttta gcattttttc
     1021 tttaaaaacc tgttcaaaag tattagtgat acataattga tgattaacaa tagcttcatg
     1081 ttcatgcctt aataatagca tcggagcttt gacaaaataa aattaaaatc actaaaatag
     1141 cacctatgca tcgctgaaat actaaagaca gtaattaaag gtggctgttt attaaggtga
     1201 atagactgta aaattatata accgatgatt cctactgatg ctatcataat gatatttaag
     1261 tacatgctcg agacaactgg tgaaaacaaa gctgaattat ccaatgatac atccacatta
     1321 atatttgaaa ataaaatata tagcggtgta taaaaaattt ctcatcatta tcacaggaaa
     1381 caaaacacaa aagcgactga attacaataa gaatgaagga atagaccatt ccatagatta
     1441 taatatactg ctattactat agatattaat aaatagatca ggcaaacaat aaatgcgata
     1501 agcgaagaaa ataataacgg atttttttat tctatacatc aatatatcct cctctaataa
     1561 acatttatgc atcaaatagc tgaacaatag atcaatcaaa aaagcaaggt agttttctat
     1621 t
//
LOCUS       DAOMTA010000761         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3953_length_1619_cov_2.218670, whole genome shotgun sequence.
ACCESSION   DAOMTA010000761 DAOMTA010000000
VERSION     DAOMTA010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_1619_cov_2.218670"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..946
                     /gene="pstC"
                     /locus_tag="PLK68_15345"
     CDS             14..946
                     /gene="pstC"
                     /locus_tag="PLK68_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRM91824.1"
                     /translation="MEKDMLSIISNRKTKSLVEKTAAIIFRGCAVMSIIAVVSITGYM
                     IISGTPAIFKVGILDILFGTVWAPTAADPQFGILLIILTSIIGVFLAICIGVPIGLFT
                     AINLAELANPKVRKIVKSAIELLAGIPSVVYGLLGILIICPFMYRLELLIFAGSKTHQ
                     FTGGANLISAILVLAIMILPTLINITETALKAVPDDYRKSSLALGASQMQTIFKVILP
                     SAKSGIVSAIVLGVGRAIGEAMAILLVAGNSVNLPLPFNSVRFLTTGIVSEMGYASGT
                     HRQVLFTIGLVLFVFIIIINLILTFIIKRGDQHE"
     gene            939..>1619
                     /gene="pstA"
                     /locus_tag="PLK68_15350"
     CDS             939..>1619
                     /gene="pstA"
                     /locus_tag="PLK68_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRM91825.1"
                     /translation="MNSIFERRHRSSDKILNFLIQLSSALSVLILVVCIGYILYRGLP
                     YFNFSYLINTTSILKGTVGILPNIINTLYIIVITLLIACPIGIGGAIYLNEYAKNKKF
                     VNIISFTTEVLAGIPSIIYGLFGMLFFGNLLGFKFSILTGSFTLAIMILPIITRNTQV
                     ALEGVPKSYREAALGIGATKWYMIRTVLVPSAMPGILTGVILGMGRIVAESAALLFTA
                     GSVSVLPKN"
BASE COUNT          488 a          217 c          315 g          599 t
ORIGIN      
        1 ataggagtta attatggaaa aagatatgtt atctattata agtaatagaa aaacaaaatc
       61 tttggtagag aaaacagccg cgataatttt tcgtggctgt gctgttatgt ctatcattgc
      121 tgttgtctct attacaggtt atatgattat ttctggtaca ccagctattt tcaaagtggg
      181 aatactagat attctttttg gaactgtttg ggcgccaacc gctgctgatc ctcaatttgg
      241 aatcctttta attatactta cctcaattat tggtgttttt ttagctattt gtattggggt
      301 accaatcgga ttgtttacag ctatcaattt agctgaattg gcaaatccga aagtgcgtaa
      361 aattgttaaa tctgcaatcg aattacttgc cggaattcct tcagtggtat atggattact
      421 aggaatttta attatttgtc cgtttatgta taggttagaa ttattaattt ttgcaggatc
      481 aaagactcat cagtttactg gtggggcaaa tctgatttca gcaattttag ttcttgcgat
      541 tatgatttta ccaactttga tcaatattac tgaaacagct ttaaaggcag ttccagatga
      601 ttatcgtaaa agttcattgg ctttaggggc aagtcaaatg caaacaattt ttaaagttat
      661 tttgcctagt gctaaatcag ggatagtttc agcaattgta ttaggagtag ggcgtgcaat
      721 tggggaagca atggcaattt tattagtagc aggaaactca gttaatctgc ctttaccatt
      781 caattcagtt cgttttttaa ctacgggaat tgtttctgaa atgggatatg cctcagggac
      841 acaccgtcaa gttttattta cgattggatt agtactattt gttttcatca taattattaa
      901 cttgatccta acatttatta ttaagagagg agatcagcat gaatagtatc tttgaaagaa
      961 gacatcgcag cagcgataaa atattaaatt ttttgataca gctttctagt gccttgtcag
     1021 tgctgatctt ggttgtatgt attggatata ttttatatcg tggattacca tactttaatt
     1081 tttcatattt aattaatact actagtattt taaaaggtac ggtagggata ctaccaaata
     1141 ttattaacac attatatatt attgtaatta ctttattaat tgcctgccca attgggattg
     1201 ggggagcaat ctatttaaat gagtatgcaa aaaataagaa atttgttaat attatttctt
     1261 ttacaactga agtattagcg ggaattcctt cgattatata tggtttattt gggatgttat
     1321 tttttggaaa tttattagga tttaaattct caatattaac gggttctttc acacttgcaa
     1381 ttatgatctt accaattatt acaagaaata ctcaggtagc tttagaaggg gtaccaaaaa
     1441 gttatcgtga agctgcattg ggtattggag ctactaaatg gtatatgatt agaactgtgt
     1501 tagtgccaag tgctatgcca ggaattttaa cgggtgtgat tttaggaatg ggtagaattg
     1561 ttgcagaatc agcggcgtta ttgtttacag ctggttcagt aagtgttttg ccaaaaaat
//
LOCUS       DAOMTA010000762         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3964_length_1617_cov_2.178617, whole genome shotgun sequence.
ACCESSION   DAOMTA010000762 DAOMTA010000000
VERSION     DAOMTA010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_1617_cov_2.178617"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..102
                     /locus_tag="PLK68_15355"
     CDS             7..102
                     /locus_tag="PLK68_15355"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91826.1"
                     /translation="MSMNEIIVANILDSGIDIVATKSIGGDELYG"
     gene            95..>737
                     /locus_tag="PLK68_15360"
     CDS             95..>737
                     /locus_tag="PLK68_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRM91827.1"
                     /translation="MAKYVMALDQGTTSSRCILFDQKGNISAMAQREFKQIFPQPGWV
                     EHNPMEIWSSQLAVASEAMALNNTKADEIAGIGITNQRETTIVWNKDTGEPIYNAIVW
                     QCRRTAAMIEQLKKDGLEEMVIAKTGLIPDAYFSASKIAWILENVDGARELANQNKLL
                     FGTVDTWLIYNLTGGKIHVTDYTNASRTMLFDIHQLCWDDELLAYFKIPKSMLP"
     assembly_gap    738..783
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <784..1612
                     /locus_tag="PLK68_15365"
     CDS             <784..1612
                     /locus_tag="PLK68_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM91828.1"
                     /translation="YGYTKEGILGGHIPVSGAAGDQQAALFGQCCYEPGEAKNTYGTG
                     CFLLMNTGNEIYHSKNGLITTIAASDNNEVSYALEGSVFVGGAVMQWLRDGLRMLKST
                     PQSSDYANRVEDTNGVYLVPAFTGMGAPYWDPYAQGTIVGLTRGCSKEHFIRAALESI
                     AYQTNDVLKAMQEDTGIELRCLKVDGGASQNDFLMQFQSDISNCKVHRPQVVETTALG
                     AAYLAGLATGFWASKEEIKNNWKLNYEFVPRINKSRREKSLEGWAKAVRFALMWKDA"
BASE COUNT          521 a          207 c          367 g          476 t
ORIGIN      
        1 gccccaatta gtatgaatga aataattgtt gctaatattt tagatagtgg gatagatatt
       61 gttgcaacaa aatcgattgg aggagatgag ctttatggct aaatatgtaa tggctttaga
      121 ccaaggaacg actagttcac gttgtatttt atttgatcaa aaaggaaata taagtgcgat
      181 ggctcaacgt gagtttaaac aaatttttcc tcaaccaggc tgggtagaac ataatcctat
      241 ggaaatttgg tcttcacaat tagcagtagc cagtgaagca atggcattga ataatactaa
      301 agctgacgaa attgcaggaa taggaataac taatcagcga gaaactacaa ttgtttggaa
      361 taaagatact ggggagccta tttataatgc gattgtttgg caatgtcgaa gaactgcagc
      421 aatgattgag cagttaaaaa aagatggttt agaagaaatg gttattgcta agactggttt
      481 aattcctgac gcatattttt cggcttcgaa aattgcttgg atcctagaaa atgtcgatgg
      541 cgctcgagaa ttagccaatc aaaataaatt actatttggt actgttgata cttggctgat
      601 ttataattta acaggtggta aaattcatgt cactgattat acaaatgctt caagaactat
      661 gttgtttgat atccaccagc tgtgctggga tgatgaatta ttagcatatt tcaaaatacc
      721 taagagtatg ttgcctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnttatgga tatacaaaag aaggaatttt gggtggacat attcctgttt cgggtgctgc
      841 tggagatcag caggcagctt tatttggaca atgttgttat gaaccgggcg aagctaaaaa
      901 tacttatgga acagggtgtt ttttgctaat gaacactggt aatgaaatat atcattctaa
      961 aaacggattg attacaacga ttgcagcaag tgataacaat gaagttagtt atgctttaga
     1021 gggcagtgtt tttgttggcg gagcagtaat gcaatggttg cgtgatggcc ttagaatgtt
     1081 aaaaagtaca cctcagtcaa gcgattatgc taatcgagtt gaagatacta atggtgtcta
     1141 tttagtgcca gcctttacag gaatgggtgc tccatattgg gatccttatg ctcaaggaac
     1201 tattgttgga ctgacaagag gttgcagtaa agaacatttt attagagcag ctttagaatc
     1261 tattgcatat caaactaatg atgttttaaa agcaatgcaa gaagatactg ggattgagtt
     1321 gagatgttta aaagtagatg gtggggcaag tcaaaatgat tttctaatgc aatttcaaag
     1381 tgatatatca aattgtaaag ttcatcgtcc acaggttgta gaaacgacag cattaggagc
     1441 agcatatttg gcaggtttag caacaggttt ttgggctagt aaagaggaga taaagaataa
     1501 ttggaaatta aattatgaat ttgttccaag gatcaacaaa tctagacgtg aaaaaagtct
     1561 ggaaggatgg gctaaggctg tacgttttgc gttaatgtgg aaagatgctt aggagag
//
LOCUS       DAOMTA010000763         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3976_length_1614_cov_1.997434, whole genome shotgun sequence.
ACCESSION   DAOMTA010000763 DAOMTA010000000
VERSION     DAOMTA010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_1614_cov_1.997434"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(770..1060)
                     /locus_tag="PLK68_15370"
     CDS             complement(770..1060)
                     /locus_tag="PLK68_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91829.1"
                     /translation="MTVKAYLEITMQIAETNRVAAAKVYTDYREPFLKTIEGALTKDL
                     LIREKDVQVLHGFDSTEHAEAYLKSEMFQKDVVVGLKPLWISDPIISIYLVV"
     gene            1220..1597
                     /locus_tag="PLK68_15375"
     CDS             1220..1597
                     /locus_tag="PLK68_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91830.1"
                     /translation="MYQHKLDKDIRCPLEYGLEIFGGKWKSRIIYVLAKKEILRYSQL
                     RKEMGNITDAVLASMLKELINNNIVTRQSYDEIPPRVEYSLTDKGKSVVPILQNICKW
                     SGVYHRENSDNTMIQCRQCDYNK"
BASE COUNT          586 a          258 c          216 g          554 t
ORIGIN      
        1 tattgcatca tataaagcat ctatttcttt aggcgttaaa acaaatattc ctttattttt
       61 ccagaggtta aatgaataat aatctacatt tgctaaagca catatcctgc tcatatgaaa
      121 atattttgat tttttcaaca gctttctttc agctgactca aatccatttc attaagtact
      181 gtcacatcag aaataacttc tccaaaatcc actaactcta acagaacaat acagtaccta
      241 ataaagtagt cagcaagctc aaaaaaagat atataatcat atctcaactg gaagatttca
      301 acgataactt ctttttgtac aaaggtaaaa cctatatatc aatatcaaca tttagaagat
      361 ggtttaataa ggatgttaaa tcttcaggtg ttaaaaaaaa atactgttca cggattaaga
      421 cactcacacg catcttattt attatctaat ccactcatat ccgaactttt aatagccgaa
      481 agacttggac atagtgttga aatgctacgt agcagatatg cccatatata tgagaaaagc
      541 agaaaaaatc ttgttgaata tattgataat ttataaaatg tataccacct atactcacaa
      601 acaactttca ttacgaacaa aaaaagcata actgacaact tcagaataag ctctatacta
      661 agacttaaat tgaataaaag ttatgctttt cttgtgtttg attttatcgc tatttattac
      721 gaagtttcat ttgtggtgta tttacagttt tgtaaacaat tacataaaat catactacta
      781 aataaatgct aataattgga tcagatatcc ataaaggctt tagtcctact acaacgtctt
      841 tttggaacat ttcgcttttg agataagctt ctgcatgttc tgtgctatca aatccatgta
      901 acacttgaac atctttttct cttattaata aatcttttgt taatgctcct tcaatagttt
      961 ttaaaaacgg ctcacgataa tccgtataaa cttttgcagc tgctactctg ttagtttccg
     1021 caatctgcat ggtaatttct aaatatgctt tgacagtcat tttcatctct ccttttttcc
     1081 tttgtacaaa catattatac aaaatcgcat atcattttaa aattcattaa gaaattaata
     1141 agttactatt aaatttctaa gtatcttgtt attataaagt atagatatta taatgaagtt
     1201 gtaaggaggg aaatattata tgtatcaaca caaattagat aaggatattc gttgtccatt
     1261 agagtacgga ttagagatat ttggaggaaa gtggaaatcg agaatcattt atgttctagc
     1321 aaaaaaagag atacttaggt atagccaatt acgaaaagaa atgggcaata ttactgatgc
     1381 tgttcttgct tctatgctta aagaattaat taataacaat atcgttacta gacaatctta
     1441 tgatgaaata cccccacgag ttgaatattc attaactgat aaaggaaaat ctgttgttcc
     1501 tattctacaa aatatttgca aatggtctgg agtttatcat cgtgaaaaca gtgataacac
     1561 tatgattcaa tgcagacaat gtgactacaa taaataatta aagccgactt attg
//
LOCUS       DAOMTA010000764         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3977_length_1613_cov_2.523748, whole genome shotgun sequence.
ACCESSION   DAOMTA010000764 DAOMTA010000000
VERSION     DAOMTA010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3977_length_1613_cov_2.523748"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>422)
                     /locus_tag="PLK68_15380"
     CDS             complement(<1..>422)
                     /locus_tag="PLK68_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91831.1"
                     /translation="CLLLGVVLFIDWHDKLVDYRAKCIIDNNEIRTECININKEISKN
                     RIYIDPKNIYKDLIDETIPPESPDMQYAEATAYCLKGTCADGTTVRDGICASKPEYIG
                     KTIIVYDKKDMSIIGYFECTDTGSEPIRNGDTIDIWIE"
     assembly_gap    423..432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(505..771)
                     /locus_tag="PLK68_15385"
     CDS             complement(505..771)
                     /locus_tag="PLK68_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91832.1"
                     /translation="MVNIFHRNAFEAKINSYGFKTVVVKGVVYVIALANKELIFLCKK
                     AAYDTKYNGSWGISTNEDTIGNVKIDKDGLKKAYSSIENIIGDK"
     gene            complement(898..1107)
                     /locus_tag="PLK68_15390"
     CDS             complement(898..1107)
                     /locus_tag="PLK68_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91833.1"
                     /translation="MNLPKMITKIRLWFYLQRCDVYNIANQLNMLSDKKADILQQRNL
                     VDVFDCYCRLGYKNVPKMKKRSKES"
     gene            complement(1191..1532)
                     /locus_tag="PLK68_15395"
     CDS             complement(1191..1532)
                     /locus_tag="PLK68_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91834.1"
                     /translation="MEYVNCRNFLKKRLVIDNVLRVLYLVLHIATIGILIYSGKYLIK
                     FFTLYLGKASLTVCFMLYMIVGMLIGYLFRKIYTKMNVEIKHTKTLIYFNEHRHLKDN
                     VITIIRNDENE"
BASE COUNT          537 a          297 c          169 g          600 t
ORIGIN      
        1 cttctatcca aatatcaatt gtatcaccgt tcctaattgg ttccgaaccg gtatcagtac
       61 attcgaaata ccctataatt gacatgtctt tcttatcgta aactataata gtctttccaa
      121 tatactctgg tttagaggca caaataccat ctctgacagt tgttccatct gcgcaagttc
      181 cttttaaaca ataagcagta gcttctgcat attgcatatc tggagactct ggcggaatag
      241 tttcatcgat tagatcttta taaatatttt taggatcgat ataaatccta ttttttgaaa
      301 tttctttatt tatatttata cattcagttc gaatttcatt attgtctatt atacattttg
      361 ctctgtaatc aaccagttta tcgtgccaat caatgaataa gacaactcct aataatagac
      421 atnnnnnnnn nngactaatt cggattagtg cacatttata tagatatttg tttcttatta
      481 tcactgtagt tacccccagt tttgttattt atctccaata atattctcta ttgaggaata
      541 tgcttttttc aaaccatcct tgtcaatctt aacatttcca atagtatctt cgttagtact
      601 gattccccaa ctcccattat actttgtatc atatgctgct tttttacaaa gaaatattaa
      661 ttctttattt gccaaggcaa tcacataaac aactccttta acaactacag ttttaaaacc
      721 ataagaatta atctttgcct caaaagcatt tctatgaaat atattaacca taacaaaatc
      781 ctccattttc aaaaagttta agagatagta aatttttaca ttcctatctc tctattatag
      841 gcctcgaatt aaccgcgaaa aaatatcttt aattataagt aaaagaaaga gcctttgtta
      901 agactcttta cttctctttt tcattttagg aacattctta taacccagcc tacaataaca
      961 gtcgaacacg tcaactaaat ttctttgctg cagaatatct gcttttttat cactaagcat
     1021 atttaactga ttagcaatat tataaacatc acacctctgt agataaaacc atagcctaat
     1081 cttagttatc atttttggta agttcataat ttaattcctc cttttctact agagcatcag
     1141 atattgacgc gaaaatagga agtatctgtt ttagacactt cccgagaata tcattcgttt
     1201 tcatcatttc taataatagt tattacatta tcttttaaat gacgatgttc attgaaatat
     1261 atcaacgttt ttgtatgctt aatttccaca ttcatcttgg tatatatctt cctaaataaa
     1321 tatccaatca acattcctac tatcatatat agcataaaac atactgttaa tgatgcttta
     1381 cctaaatata atgtaaaaaa ttttattaaa tatttacctg aatatattaa aataccaatg
     1441 gtggcaatgt gtaacacaag atacaatact cgtaatacat tatcaattac caatctcttt
     1501 tttaaaaaat tcctacaatt tacatattcc atattattag tctcctttca aaagacaaat
     1561 taattattct atattagaga ccgtattaaa cgcgaaaagt caaagtatcc gtt
//
LOCUS       DAOMTA010000765         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3979_length_1613_cov_2.009628, whole genome shotgun sequence.
ACCESSION   DAOMTA010000765 DAOMTA010000000
VERSION     DAOMTA010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_1613_cov_2.009628"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLK68_15400"
     CDS             <1..406
                     /locus_tag="PLK68_15400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91835.1"
                     /translation="NEIIKCNVSNSIFIKEPTRYIYKEYMNSEFLVLSSRAEGFGLVL
                     AEAMSCGRPCVSFNCPCGPDEIITDGVDGLLAKNGDVDDLAEKMEWMITHDKERKEMG
                     IRARESAKRYDKEKIMTQWINLFNELTNNTNI"
     gene            403..1596
                     /locus_tag="PLK68_15405"
     CDS             403..1596
                     /locus_tag="PLK68_15405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91836.1"
                     /translation="MKICFFCDSIFSFGGVQRVLAVIAKELAKSNDVTILTLDDPLLA
                     SNDIYELKNDNVNIVFFSFVEINKLSNYIHKSYSWAYKKAQLNGRCASNIYAKSSFPR
                     KQRKELISKLNEIDADVIIGVHAFLSIKLATIRKQLNAKKVIGWMHNSYYAFFEKQPA
                     YLDGIKWHFKYQMEKLDEIVVLTKTDANLYKDRLGLKTNVIYNPLTIIPGKRCDTDAK
                     KFLAVGRLSPMHKGFDILINAFAEFAKSNSEWTLDIVGDGPEKDNLLDLITAKELQDR
                     IKIRPFTKDIQSYYSAASVFILSSRWEGFPLVIMEALAHGLPIIASDIPVCREFLREK
                     DFCYLYENENVFSLCNNMTKVSSLMNLKETSINAFEFSRTHASIISIMNQWKITLNNE
                     NTTLV"
BASE COUNT          601 a          238 c          294 g          480 t
ORIGIN      
        1 taatgaaatt ataaaatgca atgtaagcaa tagcattttt ataaaagaac cgacacgata
       61 catttataaa gaatacatga atagcgagtt tcttgtatta agctctcgag cagaaggttt
      121 tggacttgta ttagcagaag ctatgtcatg tggacgccca tgtgtttcct tcaactgtcc
      181 ttgcggtcct gacgaaataa taacggatgg agttgatgga ttactggcaa aaaatggtga
      241 cgttgatgat cttgctgaaa aaatggaatg gatgattact catgataagg agcgcaagga
      301 aatgggaatt agagccagag aatctgctaa aagatacgac aaagaaaaaa taatgactca
      361 gtggataaat ttatttaatg aacttacaaa taacaccaat atatgaagat ctgttttttt
      421 tgtgattcta tctttagttt cggcggagtt cagcgtgtat tagcagtaat agcaaaagaa
      481 ctggcaaaga gtaacgatgt gacaatactt accctagatg atcctctatt ggctagtaat
      541 gacatatacg agcttaaaaa cgataatgtt aatatcgttt ttttcagctt cgtagaaatt
      601 aataaacttt cgaactatat acataagtca tatagctggg catacaagaa agcccaacta
      661 aatggacgtt gtgcttctaa catatatgct aaaagttctt tccccagaaa gcaaagaaaa
      721 gaacttatta gcaaattaaa tgaaatagat gctgatgtaa taataggcgt acatgctttt
      781 ctatctataa aattggctac tataagaaag cagctaaatg caaaaaaagt tatagggtgg
      841 atgcataatt cttattatgc cttttttgaa aaacaaccgg catatttgga tggtataaaa
      901 tggcacttta aatatcaaat ggaaaagctt gacgagatag tagttcttac aaaaacagat
      961 gcgaaccttt ataaagatag attaggcttg aaaacaaatg ttatatataa cccactgacc
     1021 attatacctg gaaaaagatg cgacacagat gctaaaaaat ttttggctgt tggacgttta
     1081 tcgccaatgc acaaaggctt tgatattcta attaatgcct ttgcggaatt tgcgaaaagc
     1141 aatagtgaat ggacattaga cattgttgga gatggtcctg aaaaagataa tttgctagat
     1201 ttaataacag caaaagagtt acaggacaga ataaaaatac gcccttttac aaaggatatt
     1261 caatcatact attcagcagc aagcgtgttt atattaagtt cacgatggga aggattccca
     1321 cttgtaataa tggaagcatt agcacacgga ttgcctataa tagcatctga tatacctgta
     1381 tgcagagaat ttctccgtga aaaggatttc tgctatctat atgaaaatga gaatgttttt
     1441 tctctttgta ataatatgac caaagtttct tctttaatga atttaaaaga aacaagtatc
     1501 aacgcatttg aattttctag aacacacgcc tcaataatat ctataatgaa tcaatggaaa
     1561 atcacattaa ataatgaaaa tactacactt gtataattga ctcattaaaa aat
//
LOCUS       DAOMTA010000766         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3984_length_1612_cov_1.398202, whole genome shotgun sequence.
ACCESSION   DAOMTA010000766 DAOMTA010000000
VERSION     DAOMTA010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_1612_cov_1.398202"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLK68_15410"
     CDS             complement(<1..830)
                     /locus_tag="PLK68_15410"
                     /inference="COORDINATES: protein motif:HMM:NF009406.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRM91837.1"
                     /translation="MIDMRKTIGDSGNVVATDNWSVAPAIFFANKQYVVPKITHPNYI
                     QEVLGICKKEDIKAIFTLIDPEIDLLASNKKKFEEIGVLPLCPSKQTADLCFDKYSMY
                     LYLKNHGVNTPLTFHELDDFKAAYFKGEIQFPVFIKPRTGSGSVGAHKVNTMQDLENY
                     YAENAFDYIIQEFMQGDCDADAYIDYYSKEVVSIFSKKKIETRIGGASKTISFKDQRL
                     FDFVQSFMHLFDFAGAIDMDFFFRDGEYYLSEINPRFGGAYLHAYGAGVDFFKLIINN
                     "
     assembly_gap    878..931
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<932..>1612)
                     /locus_tag="PLK68_15415"
     CDS             complement(<932..>1612)
                     /locus_tag="PLK68_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91838.1"
                     /translation="EAINKIQLCKNVKLGGLHLHRTSLTRSLNVYRNICKYAIRIINS
                     LGLELDYIDVGGGYFGDKPNAPTYKEYVDTIYSSLLEEGIDVSKMKVIVEPGNAIVAS
                     PFSFLSEIIDTKKIGDSQIIVTDGSRNDVDPFFLKKDYFKSFIRKQEEKVFIDSQMVV
                     GCSCLEYDKLFTLQNQPLLNVGDRILYQNVGAYTMTLSPLFIRFFPRVYLKTLNGYEI
                     IREEWTVN"
BASE COUNT          541 a          307 c          222 g          488 t
ORIGIN      
        1 atgttattga tgatcagttt aaagaaatca actccagcac catacgcatg caaataggct
       61 ccgccaaaac gaggatttat ttcagacaga tagtactctc catctctaaa aaagaaatcc
      121 atatcaattg ctccagcaaa gtcgaaaaga tgcataaaag actgcacgaa gtcaaacaaa
      181 cgctggtctt tgaatgaaat agttttactg gccccaccga tacgagtttc aattttcttt
      241 tttgaaaaaa tagaaaccac ttcctttgaa taataatcaa tataagcatc tgcatcgcaa
      301 tcaccttgca taaactcctg aatgatataa tcgaaagcat tctcagcata atagttttcc
      361 aaatcctgca tggtattgac cttatgcgct ccaacactac cactacctgt acgtggtttg
      421 atgaaaacag gaaattgaat ttcacctttg aagtatgcag ctttgaaatc atctaattca
      481 tggaaagtaa gcggtgtatt aacaccatga ttttttaaat acaggtacat actgtactta
      541 tcaaagcaca aatcagcagt ttgcttagaa ggacaaagag gaagaacacc aatttcttca
      601 aacttctttt tatttgatgc taaaagatct atttcagggt cgattaaagt aaaaatagct
      661 ttaatatctt ctttcttaca aataccaagt acttcttgaa tataatttgg atgtgtaatt
      721 ttaggaacta catattgctt gtttgcgaaa aatattgctg gggctaccga ccagttatct
      781 gtagcaacca cattaccact atcaccgata gtcttacgca tatctatcat taaacgccct
      841 ctacggcccg cattacaaca cagaatatta ttcataannn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncattgacag tccattcctc tctaattatc
      961 tcataaccat ttaaagtttt caaataaact ctaggaaaaa atcgaatgaa caatggtgat
     1021 aaagtcatag tgtatgcacc gacattttgg taaagtattc tatcaccgac atttagtaat
     1081 ggctgatttt gaagtgtaaa aagcttgtca tactccaaac aactacatcc tacaaccatt
     1141 tgtgaatcaa tgaacacttt ttcctcttgc tttctgatga aagatttaaa atagtctttt
     1201 ttcaggaaaa aagggtccac atcattacga gaaccatcag ttactataat ttgagagtca
     1261 cctatttttt ttgtatcaat gatctccgat aaaaaactaa aaggtgaagc aactattgca
     1321 tttcctggtt ccactatgac tttcatttta gaaacatcaa tcccttcctc taacaaactt
     1381 gaatagatag tgtccacata ttctttataa gtaggtgcat taggcttgtc accaaaataa
     1441 ccaccaccaa catcaatata atcaagttct agacctaatg aattaatgat ccgaatagca
     1501 tatttgcaaa tattcctata aacatttaaa gaacgagtca atgaagtacg atgcagatgc
     1561 aaacctccaa gttttacatt tttgcataat tgaattttat ttattgcttc tt
//
LOCUS       DAOMTA010000767         1611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3988_length_1611_cov_1.606041, whole genome shotgun sequence.
ACCESSION   DAOMTA010000767 DAOMTA010000000
VERSION     DAOMTA010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_1611_cov_1.606041"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..612
                     /locus_tag="PLK68_15420"
     CDS             115..612
                     /locus_tag="PLK68_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HRM91839.1"
                     /translation="MKQYYIYILEEDEEISAELIDHLSNNNNIWIEAVNNLQSTNHSE
                     VKLEHDITTILKQVGIPAHLKGYMYLRTAILMVYSDIELLGQITKRLYPNIARQYSTT
                     ASRVEKAIRHAIENSWNQGDKRKINTIIRAAPDSLKGKPTNSEFIALIADKLKIKNRY
                     AFESR"
     gene            complement(645..1046)
                     /locus_tag="PLK68_15425"
     CDS             complement(645..1046)
                     /locus_tag="PLK68_15425"
                     /inference="COORDINATES: protein motif:HMM:NF020349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HRM91840.1"
                     /translation="MILPKDQMIEKNSRDKNIFIMKRLYEIFLSIGAPNHLNGFGYLV
                     SAIKRSCSDIEILTEITKELYPSVAREYSTTSSRVEQSIRHIIKVIWSNGNLVELEKM
                     FGYRAKHRPCNSEFISMVTDRLLSEYKIYNE"
     gene            complement(1312..>1611)
                     /locus_tag="PLK68_15430"
     CDS             complement(1312..>1611)
                     /locus_tag="PLK68_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91841.1"
                     /translation="VPSSMLLVTTQIELLRSYYNHRLEEFLMVYPTIERSNAFYVVKN
                     YEKKWSKILKLTGLSLMDEEKKIIKVFIKKYQMQFMQMRSYSKIVSQLRLSEKLS"
BASE COUNT          571 a          265 c          195 g          580 t
ORIGIN      
        1 acatgaatct tattacctag tttactaaaa gaaaataaat aataacaatt ccccttttac
       61 taaaatcatt gtctttatat aatagttttt gtcattattt ggaaggagaa taatatgaaa
      121 caatactaca tatatatttt agaagaagat gaagaaatat ctgctgaact gattgatcat
      181 ttatcaaaca ataataacat ttggatagaa gcagtcaata atcttcagtc cacaaatcat
      241 agtgaagtta aacttgaaca tgatattaca acaattctaa agcaggttgg cattccggct
      301 cacttgaaag gctacatgta tttaagaact gctattttaa tggtatactc tgatatcgaa
      361 ttattaggtc aaataacaaa aagactttat ccaaatattg ctcgccaata cagtacgaca
      421 gcctcaagag ttgaaaaagc aattcgtcat gcaattgaaa acagttggaa tcaaggtgac
      481 aaaagaaaaa ttaataccat tattagggcc gccccagatt ctcttaaagg aaaaccaaca
      541 aattctgaat ttattgcttt aattgcagat aaactcaaaa taaaaaatcg ttatgcattt
      601 gaaagcagat aaaaagaagt tttatatgaa cttcttttta taaattactc attatatatt
      661 ttatattctg acaacagtcg atcagttacc attgaaataa attctgaatt acaaggtcga
      721 tgtttagctc gataaccaaa cattttttct aactctacaa gattaccgtt tgaccaaata
      781 accttgatta tatgtcggat tgattgttcc acccgtgacg aagttgtaga atactctctt
      841 gctacacttg ggtataattc ttttgtaatt tctgtcaaaa tttcaatatc actgcatgaa
      901 cgttttattg ctgaaactaa ataaccaaat ccgtttaaat gatttggtgc acctatcgat
      961 aaaaatattt catatagtcg tttcattata aatatatttt tatctctact attcttttct
     1021 atcatttgat cctttggtaa gatcacaata ctaaattccc tattcatttt taataactta
     1081 gtgtctaata cacttatctc ctcactttaa actttatccc atattgaatc tgtttttgag
     1141 cgcattaaag cccttatccc gagataacct tgtacttcac taagaattta tatcttaata
     1201 tattttttat atcaaaataa ttcattatac tcactcccct ctctttatcc caggattagt
     1261 ataacaaatt taggcccttt aaaatataaa aattatgtgt attgatcaaa tttagctaag
     1321 cttttcactt agccgtaatt gagatactat ttttgagtat gatctcatct gcataaattg
     1381 catttgatat ttttttataa atacttttat tatcttcttt tcttcatcca ttaacgaaag
     1441 acctgttaat ttcaaaattt tcgaccattt tttttcataa tttttaacta cataaaaagc
     1501 attagaacgt tcaatggttg gatataccat caaaaattct tcaagtctat gattataata
     1561 tgaacgcaac aattcaattt gagttgttac tagtaacatt gacgaaggaa c
//
LOCUS       DAOMTA010000768         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3992_length_1610_cov_2.562701, whole genome shotgun sequence.
ACCESSION   DAOMTA010000768 DAOMTA010000000
VERSION     DAOMTA010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3992_length_1610_cov_2.562701"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..769
                     /locus_tag="PLK68_15435"
     CDS             11..769
                     /locus_tag="PLK68_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM91842.1"
                     /translation="MTNIMLEVKNLKKIYDELGPSPKIALDDLSFEIKNNEFVCIMGP
                     SGSGKTTLVNILSTIDKATSGIVNISGASIVGMSGAVKAKFRKKKLGFIFQNYNLLYS
                     LTIRENILFPLIINSIGEKEWQENLEQVTQILGIKDILDKHVYECSGGQQQRAAIARA
                     LISKPEIIIADEPTGNLDSNNSRELMELFSKINHDSQTTIIMVTHDAFVASYSTKMLY
                     MKDGKIDKILNRHGLTRDEYFNEIVKVNAILSNN"
     gene            784..1329
                     /locus_tag="PLK68_15440"
     CDS             784..1329
                     /locus_tag="PLK68_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91843.1"
                     /translation="MMRIKLYLGSQELEKYLNKKVQHNYILETIIPFGLFQPLRLDVL
                     KFKKKKDLQRIYIVDSNDLVLIKRKYQNLGWQYFENNYANDEFNNDAIFYYDSIDEKE
                     LMKMNIKESCHQNDVSASLVKGLFFGTVFLIFSVICPNLMIHVNNNGLDFILGNLYII
                     TALILIVMAVIGKLRNKKRKE"
     gene            complement(1347..>1610)
                     /locus_tag="PLK68_15445"
     CDS             complement(1347..>1610)
                     /locus_tag="PLK68_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRM91844.1"
                     /translation="IASWIEPNGGYFISLDVMPGCAKRIGELCKDAGVVLTTIGATYP
                     YGIDPQDTNIRIAPSYPSVDELSKAAELLCICVQLACLEKLLK"
BASE COUNT          587 a          189 c          283 g          551 t
ORIGIN      
        1 gaaattaaaa atgacaaata ttatgcttga agttaaaaat ttaaagaaaa tatatgatga
       61 actaggtcct tcaccaaaaa ttgcgttaga tgatcttagt tttgaaatta aaaataatga
      121 atttgtttgt attatggggc cttctggttc tggaaaaacg accttagtaa atattttatc
      181 aacaattgat aaagcaactt caggaattgt taatatttca ggtgcaagta ttgttgggat
      241 gagtggggca gtaaaggcaa aatttagaaa gaaaaagtta ggttttattt ttcaaaatta
      301 taatttactt tattctttaa cgattcgtga aaatattctt tttccgttaa ttataaatag
      361 tatcggtgaa aaagaatggc aggaaaattt agagcaggtt acacagattt taggtattaa
      421 ggacatactc gataaacatg tatatgagtg ctctggtggg caacagcagc gagcggcaat
      481 tgcaagagcc ctaattagta aacctgaaat aattattgct gatgaaccaa ctggtaatct
      541 tgatagtaat aattctagag aattgatgga attattttcg aaaatcaacc atgattctca
      601 aacaacaatt attatggtga ctcacgatgc atttgttgcc tcgtattcaa ctaaaatgtt
      661 atatatgaaa gatggtaaga tcgataaaat tttaaatcga catggtctaa cacgagatga
      721 atattttaat gagattgtta aagttaatgc aatattatct aataattgaa aggaatgatt
      781 gaaatgatga ggatcaagct ttatttagga agtcaggaac tagagaagta tttaaataaa
      841 aaagtacagc ataattatat tttggaaact attattcctt ttggtctttt tcaaccttta
      901 agattagatg tattaaaatt taaaaagaaa aaggatttgc aacgtatcta tattgttgat
      961 tccaatgact tagtattaat caaacgtaaa tatcaaaatt tgggatggca gtattttgaa
     1021 aataattacg ctaatgatga atttaataat gatgcaatct tctattatga tagtattgat
     1081 gaaaaagaat tgatgaagat gaatattaaa gagtcatgtc atcaaaatga tgtatcagcg
     1141 agtctagtaa aaggtttatt tttcggtaca gtatttttaa ttttttcagt tatttgtcca
     1201 aatctaatga ttcatgtaaa taataatggt ttagatttta ttttagggaa tttatatatt
     1261 attactgctc tcatattaat agtgatggct gtaattggaa aattaagaaa taaaaagcga
     1321 aaggaataag tttcctttcg ctaagtttat tttaatagtt tttctaaaca agctaattgc
     1381 acacagatac atagtagttc agcggctttt gataattcat caactgaagg atatgatgga
     1441 gcaattctaa tgttagtatc ttgtgggtcg attccatatg ggtaagttgc tccgatagtt
     1501 gttaaaacga caccagcatc tttacataat tccccaattc gtttagcaca tccaggcatt
     1561 acatcgagac tgataaaata tccgccattt ggttcaatcc atgaagcaat
//
LOCUS       DAOMTA010000769         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_3999_length_1608_cov_2.205409, whole genome shotgun sequence.
ACCESSION   DAOMTA010000769 DAOMTA010000000
VERSION     DAOMTA010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3999_length_1608_cov_2.205409"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..635
                     /locus_tag="PLK68_15450"
     CDS             453..635
                     /locus_tag="PLK68_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HRM91845.1"
                     /translation="MENKCLSVKYYECVMLLENNVIEIKMANNTLKVTGADLEIRYYS
                     DQEVIIYGKIDCLRFL"
     gene            617..1552
                     /locus_tag="PLK68_15455"
     CDS             617..1552
                     /locus_tag="PLK68_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HRM91846.1"
                     /translation="MLKISMRLGYDLCEVVSEDIVGLLKSFKENHITVFQLTKVDDCT
                     YRFYLPIYQRIMVRKYHLTIIKSVGILYYLILMFYRKLSIVGVVSFAVTVILCNQFIF
                     RVEIIGNNPSTTKLVNQVLAENHIDVGDKKRTYQQLNDIYDDLKDSFKGKIDYLNIYQ
                     EGGVLFVKYTNSVGAKKVEKNFENIYASKDGVIQKIDVSSGNILVKLNQYVKKGDLLV
                     SNTITSTSEVDKIIETEGTIKAYTYTTYEAKIDKKKMDDGEAFSYLLHTIRSKLGSID
                     KIDREKVLSYGIIDNKRVLKVQYILIEDIASKEEN"
BASE COUNT          629 a          154 c          268 g          557 t
ORIGIN      
        1 tgattgttgt gaaacacaaa gccgaaatct ccaacaataa aatatcacgt ttagttaaat
       61 agtgattaaa cacattaaaa caaccttgat ggtcgagggt atctgcggta atataaatcg
      121 ttttttctcc ttaaaaaatc tattgctttg attaaaaatt gcataataaa cacattgata
      181 attttattaa gcaagattaa atcatgagta tttgacgatt aactaataat gtaaaaaatg
      241 caaaattaat ttcttcaaaa tctttactag tatcactatc attttttatc attacatata
      301 agtttaagct acttttgtta ataggtatgt tatatgcatc tctaaaatga ttttagtata
      361 aaaattatta tagtgaaact attttattac acaattaaac gcatttacta aaaaaatatt
      421 catataatta gttaggtgat tcgatgatct atatggaaaa taagtgttta agtgtcaaat
      481 attatgaatg tgtgatgctt ttagaaaata atgttattga gataaagatg gctaataata
      541 ctcttaaagt aactggcgca gatttagaaa taagatatta tagtgatcaa gaagtaataa
      601 tttatggaaa gattgattgc ttaagatttc tatgagatta ggatatgatt tatgtgaagt
      661 cgtcagtgag gatatagtag ggttattgaa atcatttaaa gagaaccata ttacagtatt
      721 tcaattaaca aaagtcgatg attgtacata tcgattctat ttgcctattt atcaacggat
      781 aatggttcgt aaatatcatt taacaattat caaaagtgtt ggaatactgt attatttaat
      841 tttaatgttt tatcgtaaat tgagcatcgt tggagttgtc agttttgcag tcactgtgat
      901 tttatgtaat caatttattt ttagagttga gattatcggt aataatccta gtacaactaa
      961 attagttaat caggttttag ctgaaaatca tattgatgtt ggtgataaaa aaagaactta
     1021 tcaacaatta aatgatattt atgatgattt aaaagatagt tttaaaggga agatagatta
     1081 tttaaatatt tatcaagaag gtggcgtatt atttgtaaaa tatacaaata gtgttggagc
     1141 taaaaaagta gaaaagaatt ttgagaatat ttatgcaagt aaagacggag taattcaaaa
     1201 aattgatgtt agcagtggaa atattttggt taagttgaac cagtatgtaa aaaaaggtga
     1261 tctgctagtt tctaatacga ttacttcgac tagtgaagta gataaaatta ttgaaaccga
     1321 aggaacgatc aaagcatata cgtatactac ctatgaggcg aagatcgata agaaaaaaat
     1381 ggatgatggt gaagcattta gctatcttct ccatacaatt cgtagtaaac taggttcaat
     1441 cgataaaata gatagagaaa aagttttatc ttatggtata attgataaca aaagagtatt
     1501 aaaggtacaa tatattttga tagaagatat tgcatctaaa gaggagaatt aaatgacaga
     1561 acaagttaaa ttagaagaat atagtattgc gcaattaaat aatatttg
//
LOCUS       DAOMTA010000770         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4001_length_1607_cov_2.538660, whole genome shotgun sequence.
ACCESSION   DAOMTA010000770 DAOMTA010000000
VERSION     DAOMTA010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_1607_cov_2.538660"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            853..1230
                     /locus_tag="PLK68_15460"
     CDS             853..1230
                     /locus_tag="PLK68_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91847.1"
                     /translation="MDTTIVYRKRRYYCKQCKTYHYEYNPFTAQTKSRFTDLSTIRIM
                     DSLREHSATFSMVARQFNTTPTKVINIFDKLGQMKKLPFTEIVSLDEFYWNRKSKNKY
                     ACAILDFKTGNIIDILNGRTLKS"
     gene            1288..>1607
                     /locus_tag="PLK68_15465"
     CDS             1288..>1607
                     /locus_tag="PLK68_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM91848.1"
                     /translation="MDLYETYRQVQKIYFPKAILCCDSFHVIKNINLILKNERIKIMK
                     RYNTDSVEYYLLKKFNFLLMKDSSKIEYIKAKYNKKLKRYINYPQILYLIFDYPHKHY
                     TFKY"
BASE COUNT          595 a          221 c          202 g          589 t
ORIGIN      
        1 aagaatgata cttttattct acagaaacaa ccatttctaa tgattatttt ttatttttct
       61 tataatttcc tctttctttt ccaggctctc tatttgtctt tcttgacata gtaccattct
      121 ttctatttgt caatagaggt aaggaatctt tattaataca gtacattata cggcttctta
      181 atctttcaaa gttcttatat tttaatgatg ttttcaaaag aacttttatt cttccgttaa
      241 ttgattctat tggacctgat gctagtataa ttgtagcgtt ggaaatcctt tgtctagtag
      301 caataaaagg attttcttca agaaaagact tatgacaatc aatgcatata aatcttctgc
      361 atctgtaatt gatataggtt ttatttacgt tgaaaataga atgagtgatt ttttaatctt
      421 gtaatccttg atatttgcat ttaaactgcc tcaacaggga caaggcttaa tttttctggt
      481 taaagtgaca ttaataaaaa cattattatc agttcttaca atgtttatag gggtttacac
      541 ccattccttt aattcattgg ggtcatcata ttttagatga cctttttttg ataataaaat
      601 ttcttccgtg gcatgatttg agtgcttaat tcaatctatc acaaaaagag taatatcatg
      661 tcagatgata tcgttaatat tttaaatctt aattactctg atgtacagtc ttgtacataa
      721 aaaaacaaaa ataatcacat tacttatttt gttaccttga accaaaaaga tttcgtatgt
      781 tcttaatgct tcaataggat tagtataaaa gactacagag aagtaaaaat aagccaccaa
      841 atcattcgtg agatggatac tactattgtt tacagaaaaa gaagatatta ttgtaaacaa
      901 tgtaaaacat atcactatga atataaccca ttcacagctc aaacaaaatc acgttttact
      961 gatttatcta caattagaat aatggattca cttagagaac attcagctac tttttctatg
     1021 gtagctagac aatttaatac tacaccaact aaagttataa atatatttga taaattagga
     1081 caaatgaaaa agttaccttt tactgaaatc gtttctctag atgaattcta ttggaacaga
     1141 aaaagtaaaa acaaatacgc atgtgcgata ttagatttta aaacaggtaa tattattgat
     1201 attcttaatg gaagaacttt aaaaagttga gatagatata ctcaactact tgataaagaa
     1261 gaactcaata aagttaagta tattagtatt gatttatatg aaacatatag acaagttcaa
     1321 aaaatctatt ttcctaaagc tattctatgt tgcgattcat ttcatgtaat taagaatatt
     1381 aatcttattc tcaaaaacga aagaattaaa ataatgaaaa gatataacac tgatagtgtt
     1441 gaatactatc tgttaaagaa gtttaatttt ctgcttatga aggattcatc aaaaattgaa
     1501 tatatcaaag ctaaatacaa caaaaaacta aaaagatata ttaattatcc tcaaatttta
     1561 tatctaattt tcgattatcc gcataaacac tatactttca agtattt
//
LOCUS       DAOMTA010000771         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4005_length_1607_cov_1.557345, whole genome shotgun sequence.
ACCESSION   DAOMTA010000771 DAOMTA010000000
VERSION     DAOMTA010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4005_length_1607_cov_1.557345"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLK68_15470"
     CDS             complement(<1..230)
                     /locus_tag="PLK68_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRM91849.1"
                     /translation="MEILYNILDAYAYEIVFVDRNHIVRYMNKTAKQRYGSRVQVGNS
                     LFNCHNENSKKRIEEFLERADAGEEEMFEVLNG"
     gene            469..633
                     /locus_tag="PLK68_15475"
     CDS             469..633
                     /locus_tag="PLK68_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91850.1"
                     /translation="MKKKLVVLLTVRIILWRFFITYLVGIDIAKYKHDCFIHDHNGEA
                     IRHSFTFVNN"
BASE COUNT          532 a          244 c          220 g          611 t
ORIGIN      
        1 ccattaagaa cttcaaacat ttcttcttca ccagcatcgg cgcgttctag aaattcttca
       61 attcttttct ttgaattttc attatgacaa ttgaataaac tattgccaac ttgaactctt
      121 gagccgtatc tttgctttgc ggttttattc atatagcgta caatatgatt tcgatcaacg
      181 aagactattt cataagcata agcatctaag atattatata agatttccat tgttatttcc
      241 attttatcac gaatactcaa tttaggtcac taagttgagt attctccttt ctgcccttat
      301 atacatttat atcatccagg gccaatgatg atataatttt attagcactg tggatttgtc
      361 tctatttttc tacagcttga ctgtttagag gttatacctg ccacagttct ttttcaaatt
      421 gttgattaag ttaattagca ctcgatgctt tatgaacaac gtgaatacat gaagaaaaaa
      481 cttgtggtct tactgacagt gcgaattatt ttatggaggt tttttataac ttatttagtt
      541 ggtatcgata ttgctaaata caagcatgac tgcttcattc atgatcataa tggtgaagcc
      601 attcgtcatt cttttacttt cgttaataac tagaatgggt tcaatcaatt gcttcttatt
      661 cttaaatttt ttggataaga atcaaaaaat aaaaataggt tttgaagcca ctggtcatta
      721 tggaagcagc cttaaacaat tcctcaaagc ctacaatttt gactttatgg agattaattc
      781 ttttttaatc aaacaatttt ccaaagcctc aactcttagg aaaactaaaa ctgataagat
      841 tgattctgtt ttgattcctt gatatcttac gaccgttgac tacaaaccta cttcgaacca
      901 gtcatagcac cttgcttgaa atctacaaaa atattaatgc taaaataaaa gatattgaag
      961 ctggaatcat tcagaaatga gtctggacaa gataatttta aaggtcacat ggcaaaacat
     1021 ggctcttctc atcttcgtca aattatcatg aatgttgtag atacttttgc attaaacaat
     1081 cctgttatat atgactatca atcaaagaag gaaaacactg gcgagttgca cagactcacg
     1141 tttgctagaa aacttatccg tatcattttt tacctagaaa aaacggcatg gattttgatt
     1201 ccaaattatt aaaataataa tcaatagttg tcactattaa tttttttgga gttgaaaaac
     1261 tcctttttgg tgttatttga tatctatata taaatttttt tataaatcgt ttgatttaca
     1321 atagttaatc acctcttact atttgatttt aacaaatatt cattattttt gatagtcatt
     1381 gtataaatga gagacatatt aaataaaatt attgactatt attgtggcta agttacaata
     1441 gtaatttgaa tttaaataaa agtaaatagt atactaacat ctttgaaatt ggtcaattag
     1501 ttttaaatac taaaatcata tatgtatttt atattaactc ttgtggaaat aatggcatat
     1561 gtgaatcatt tttgatatat cacaaattta ttttttattt ttatatg
//
LOCUS       DAOMTA010000772         1604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4012_length_1604_cov_2.096837, whole genome shotgun sequence.
ACCESSION   DAOMTA010000772 DAOMTA010000000
VERSION     DAOMTA010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4012_length_1604_cov_2.096837"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLK68_15480"
     CDS             <1..208
                     /locus_tag="PLK68_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRM91851.1"
                     /translation="YPKLEVKNYEIISFAPSKVVIDDLDDNEYYNVLISMEFNRKVDE
                     LPKSVNIVLVLDNDRFYVVGVDNA"
     gene            201..680
                     /locus_tag="PLK68_15485"
     CDS             201..680
                     /locus_tag="PLK68_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM91852.1"
                     /translation="MLSYLKVENIDNELIEFHLRQKNYYSLEGQVVTREYLINEMRCP
                     DGFDEIDHYIEKCYLEGELVGLVDYQYGYRYSMIHDDECVWIGLFLIDQDKQNCGLGK
                     RLFNDCLKKFKQRCERIQLACLVRNEAGLVFWKKCGFNEIGNSKYGDLPVIVLEKRI"
     gene            794..1024
                     /gene="secG"
                     /locus_tag="PLK68_15490"
     CDS             794..1024
                     /gene="secG"
                     /locus_tag="PLK68_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRM91853.1"
                     /translation="MFGTILNVLLVVISIALIILCLLQSGKSDGIVNALTGQSSNLFA
                     QQKERGADLVMTRVTTGLAIAFFVIAIILRMS"
     gene            1074..>1604
                     /locus_tag="PLK68_15495"
     CDS             1074..>1604
                     /locus_tag="PLK68_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRM91854.1"
                     /translation="MKEKILELLSDENYLERSIDLIAAKLNCNKSDKFVNLVKLMNEL
                     EDEGKVVRNKYNHYYLPNQFGMILGTLTINKRGFGFVVVEDQEKDIFISPDALKDAFN
                     KDTVLVELKKHSDEERPEGRVIRVIKRGQTRLVGEIKKGKRDCFVDVDDPMFDKPIFV
                     DRAHLHGAMPGHKVQVE"
BASE COUNT          549 a          148 c          346 g          561 t
ORIGIN      
        1 gtatccaaaa ttagaagtta agaattatga aattatttct tttgcaccat ctaaggtagt
       61 gattgatgat ctagatgata atgaatatta taacgtatta atctcaatgg aattcaatcg
      121 taaggttgac gaattaccta aaagtgtaaa tatagtttta gttttagata atgatcgttt
      181 ttatgtagtt ggtgttgata atgcttagtt atttaaaagt agagaatatt gataatgaat
      241 tgattgaatt tcatttacgt caaaagaatt attattcttt ggaaggacag gtagttacta
      301 gagaatatct tattaatgag atgcgatgtc ctgacggttt tgatgaaata gatcattata
      361 tagaaaaatg ttatttagaa ggtgaactag ttggattagt cgattatcaa tatggctatc
      421 gttattcgat gattcatgat gatgaatgtg tatggattgg attattttta attgatcagg
      481 ataaacaaaa ttgtggattg gggaagaggc tgtttaatga ctgtttaaaa aaatttaagc
      541 aacgttgtga acggatccaa ttggcttgtt tagtgaggaa tgaagctggt ttagtatttt
      601 ggaaaaagtg tggatttaat gagattggta attctaaata tggtgattta ccagtaattg
      661 tattagagaa aagaatatag taggcagctg tcaagaagat ttgattaaag tgtcgatgaa
      721 atcattgtaa ttttaaaaat attatgttat tatgttctag cgatgtatca aagtatctaa
      781 taggaggtat attatgtttg ggactattct aaatgtttta ttagttgtta tttcaatagc
      841 acttattata ttatgtctat tacaaagtgg taaatctgat ggaattgtaa atgctttaac
      901 tggtcaaagc tcaaacttgt ttgcacagca aaaagaacgt ggtgcagatt tagtgatgac
      961 aagagttact actggtttag caattgcatt tttcgttatt gcaatcattc taagaatgtc
     1021 ataaataata gggctaaggt cctattttta ttttggaatc gggaggtgaa tgaatgaaag
     1081 aaaagatttt ggaattgtta agtgatgaaa attatttaga gcgttctatt gatttgatcg
     1141 ctgctaaatt gaattgtaat aaaagtgata aatttgtaaa cttagttaaa cttatgaatg
     1201 agttggaaga tgaaggtaaa gtagttagaa ataaatataa tcattattat cttccaaatc
     1261 agtttggcat gattctagga acattaacga ttaataaacg tggttttggg tttgtagtcg
     1321 ttgaagatca agagaaagat atttttatta gtcccgatgc cttaaaggat gcgtttaata
     1381 aggatactgt attagttgaa ttaaagaagc acagtgatga ggaacgtcct gaggggcgag
     1441 ttatcagggt tataaaaaga ggacagacaa gattagttgg tgaaatcaaa aaaggaaaac
     1501 gtgattgctt tgttgatgtt gatgatccga tgttcgataa accgattttt gtagatcgcg
     1561 ctcatttaca cggagcgatg ccgggacaca aagttcaagt tgaa
//
LOCUS       DAOMTA010000773         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4027_length_1601_cov_1.851876, whole genome shotgun sequence.
ACCESSION   DAOMTA010000773 DAOMTA010000000
VERSION     DAOMTA010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_1601_cov_1.851876"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..629)
                     /locus_tag="PLK68_15500"
     CDS             complement(168..629)
                     /locus_tag="PLK68_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91855.1"
                     /translation="MVYTFMNYDDFNSKYNDILKIATCYSENFSFFTVRQIHKKDHSN
                     EYFNFLDGFRNHEIPLGKIKTPRYTAGQQIHCFRINKITTALLMKPIDFSSWNAYDYP
                     EDLAFYKNCIPWFRCISHEKMVLVNETNFRAFNELKKIGINFRLINNSNYI"
     gene            complement(647..1219)
                     /locus_tag="PLK68_15505"
     CDS             complement(647..1219)
                     /locus_tag="PLK68_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91856.1"
                     /translation="MNYLDPNGYVALVDDLVYALIALTAATVAICSTSFFQKGWSAFC
                     NAVGNGLSSIGNAIWNGASATWNWSKNKVKNAINAVKKFNTAVKSANNIRSKLKKERK
                     NNKRFYTITFNSDDVPILGSKLTKSKVESKLRQGKDVITYYKSDALNIANSVGSTRSK
                     CDPKHRGSASFKYYHVKYKNIKWSIHSFYV"
     gene            complement(1216..>1601)
                     /locus_tag="PLK68_15510"
     CDS             complement(1216..>1601)
                     /locus_tag="PLK68_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91857.1"
                     /translation="SDKSVQMSYDSNGMRTQKTVDGVKTNYYYDSNKNLIALVKGNDT
                     LLFYYDSDGSATSFSYNGTMYFYVKNLQGDVIRIIDLAGTEVASYVYDAWGNIKDAKG
                     DPTVRELNPIRYRGYVYDTETDLYY"
BASE COUNT          510 a          289 c          243 g          559 t
ORIGIN      
        1 aatcggtaac aatataatga gtaagactgc cgtcagatga tgtgcgatat tcgctttcaa
       61 gcgaaccgtc aaatgtgtag tagtaatatg aggtttcgcc gcttgaataa ttttaggtat
      121 cagtaggctc aaatgagcct acttacccac attcgtttta aattttttca aatatagtta
      181 gaattattaa tcaatctaaa atttatgcct atttttttta actcattaaa tgctctaaaa
      241 ttggtctcat tgactaacac cattttttca tgtgaaatac agcgaaacca cggtatgcaa
      301 tttttataaa aagccaaatc ttcaggataa tcataagcat tccaagaaga aaaatctata
      361 ggtttcatca ataaagccgt tgttatttta ttaattctga aacaatggat ttgttgtcct
      421 gcagtatatc tgggtgtttt tattttgcct aatggaattt catgatttct aaaaccatcc
      481 aagaaattaa aatattcatt agaatgatcc tttttatgga tctgtctcac agtaaaaaaa
      541 ctaaaattct cgctatagca agtggcaatt tttaatatat cgttatattt actattaaaa
      601 tcgtcataat tcataaaagt ataaaccata ataccacctg tatgccctat acataaaacg
      661 aatggatact ccactttata tttttatatt taacatgata atacttaaaa gaagcagacc
      721 ctctatgttt gggatcacac ttacttctag tgctgcctac tgagtttgcg atatttaacg
      781 catcactctt ataataagtt attacatctt tgccttgccg caactttgat tcaactttac
      841 ttttagtaag tttacttcca agtataggta catcatcact attaaatgtt atagtataaa
      901 atcgtttatt gttttttctt tcttttttca atttggatcg tatattattt gctgatttaa
      961 cagcagtatt aaatttttta actgcattaa ttgcattttt aaccttattt ttagaccaat
     1021 tccatgtagc agacgcacca ttccaaattg cattaccgat agatgatagt ccgttaccga
     1081 ctgcgttaca aaaagcagac caaccttttt ggaaaaatga agtggaacaa atcgctacag
     1141 ttgctgcagt taaagctatt aaagcataaa ctaaatcatc aactagggct acatacccat
     1201 taggatcaag ataattcaat agtacaagtc tgtttcagta tcataaacat agccacgata
     1261 acgaattggg ttcagttcac ggactgtcgg gtcacctttg gcatctttta tgttgcccca
     1321 agcgtcataa acatagcttg caacttctgt acctgcgagg tcaataattc tgattacatc
     1381 accctgcaag ttcttcacat aaaagtacat tgtaccgtta tatgagaatg aggttgcact
     1441 tccgtcactg tcatagtaga acaacagagt gtcattaccc tttacaagag caattaaatt
     1501 tttgttgctg tcgtagtaat agttggtctt aactccgtca acggttttct gtgttctcat
     1561 accgtttgag tcatagctca tttgaacgga tttgtcggaa g
//
LOCUS       DAOMTA010000774         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4030_length_1601_cov_1.587969, whole genome shotgun sequence.
ACCESSION   DAOMTA010000774 DAOMTA010000000
VERSION     DAOMTA010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_1601_cov_1.587969"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLK68_15515"
     CDS             complement(<1..842)
                     /locus_tag="PLK68_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91858.1"
                     /translation="MKSIRSILHILLGVTLICSCNEATELAHITPNVSLIKYKDIVKI
                     DSLYTYPGDSLYQNWINQYNKNLEDKTSRSLSPEDDAFFNKQYIVKSTEATVLYGRNY
                     ILPGSILEGNSISNQNYIPVFISNRKPITVSMTLAHNTPKPTSRTIEAPTFSKLSDYV
                     VEMVTDGNFEQNQKFMFSYKRFSFYDEIKTAFGTNINTRKLFSSKSESSTEYRDKIQK
                     STGMYVKFFQSSFTVNMDIAPLSDQPIQGKSEYEPVYVNSLTYGRLGIIAFETDESYE
                     FAET"
     gene            complement(855..>1601)
                     /locus_tag="PLK68_15520"
     CDS             complement(855..>1601)
                     /locus_tag="PLK68_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91859.1"
                     /translation="INYLSIFCNPRNQDTYKFLKISMNPINMGYTIPIKSQTITPSYN
                     NMMKAIHEALKTNDVLKSNYSFSFVIKKIKSYQELAVNWGQNINLQQLLGITPDYKPQ
                     KNIILIDINQSFFSIIMDMPESLSTDPQVLQQLDKLAFINSIQFGRKVILVIESNIDY
                     DKLQETIDNLLKSKEVSQKELAILANSNIRLMTIGNKEIKDINPDNPFTSILTYLSST
                     VTPDDFGGPISFSASNIKDNSVFVNYFNVQ"
BASE COUNT          466 a          225 c          280 g          630 t
ORIGIN      
        1 cacgtttctg cgaattcgta tgattcgtct gtttcaaagg caatgattcc cagtcttcca
       61 taagtcaatg aattcacata aacaggctca tattcacttt ttccttgaat aggttggtca
      121 cttaaaggag caatatccat gttgacggtg aaacttgatt ggaagaattt aacatacatt
      181 cctgttgact tttgtatttt atccctgtat tctgttgaac tttcactttt tgaagaaaat
      241 agttttcttg tattaatatt tgttccgaat gcggttttaa tttcatcgta aaagctaaat
      301 ctcttgtaac tgaacatgaa tttctgattc tgttcgaaat ttccatcggt taccatctca
      361 accacataat cacttaattt agagaaagtg ggtgcttcta tagttctgct agtgggtttg
      421 ggtgtattgt gtgccaatgt cattgatact gttatgggtt ttctgttgct gataaatacc
      481 ggtatataat tttggtttga aatgctattt ccttctaaaa tggatcctgg aagtatgtaa
      541 tttcttccat ataaaacggt tgcttctgta cttttcacaa tatattgttt attgaaaaat
      601 gcatcatctt ccggtgaaag tgatcttgat gttttgtctt ccagattctt attatattga
      661 tttatccaat tctgatatag agaatctcct ggatatgtat ataaagagtc tattttaaca
      721 atatctttat attttataag actgacatta ggggtaatat gggctaattc tgttgcttca
      781 ttgcatgagc agattagcgt tacgcctaga agtatgtgta aaatagaacg gatgcttttc
      841 ataatgatga ttatttattg tacattgaaa tagttaacaa atacagaatt atcttttatg
      901 ttagaggcgg aaaatgatat aggtcctccg aaatcatcgg gtgtaactgt tgaagataaa
      961 taagtaagta tgcttgtaaa aggattgtct gggttgatat cttttatttc tttgtttcca
     1021 atagtcatca atctgatatt agaattggct aatattgcta gttctttttg actaacttcc
     1081 ttagacttca gtaaattgtc aatagtttct tgtaatttgt cataatcaat attactttca
     1141 ataactagta taacttttct gccaaattgt attgagttta taaatgcaag cttgtctaat
     1201 tgttgcaaaa cttgtggatc agtgcttagt gattccggca tatccattat aatgctgaaa
     1261 aaactttgat ttatatctat caatataata ttcttttgag gcttataatc gggtgtgatg
     1321 cctaatagtt gctgaaggtt aatattttgt ccccaattga cagctaattc ttggtatgat
     1381 tttatctttt taattacgaa tgaaaaagaa tagttacttt tcaaaacatc gtttgttttt
     1441 aacgcttcat gaatagcttt catcatgtta ttataggaag gagtgattgt ttgtgacttt
     1501 ataggaatgg tgtaccccat gtttatagga ttcattgata ttttcaggaa cttatatgtg
     1561 tcttgatttc gaggattaca aaagatggac aaatagttta t
//
LOCUS       DAOMTA010000775         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4034_length_1600_cov_2.173463, whole genome shotgun sequence.
ACCESSION   DAOMTA010000775 DAOMTA010000000
VERSION     DAOMTA010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4034_length_1600_cov_2.173463"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLK68_15525"
     CDS             <1..396
                     /locus_tag="PLK68_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HRM91860.1"
                     /translation="ARSLENFRTELNVLLKNKNVLIKSSLINLFKLLIMYSIPFFAAK
                     ALNLNVSFIQIFDFIGICSFVYMITAFVPIPGASGGSEGVYYMLFSPILGAVGTPTTL
                     LVWRFVTYYLGLIIGGIIFATNREINRSE"
     gene            401..>1600
                     /locus_tag="PLK68_15530"
     CDS             401..>1600
                     /locus_tag="PLK68_15530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM91861.1"
                     /translation="MRIAIFSDTYIPDINGVATSTKILKDELVKHGHDVVVVTSELPS
                     DSDYMDDPHDNILRVPGLEIQALYGYRACNIYSFKGMREIKGMNIEVIHVQTEFGVGI
                     FGRIVGETLNIPVVYTYHTMWADYSHYVNPVNSGAIDGLIKKAITRISKFYGDKSTEL
                     IVPSLKTKEALELYGLNKDVHIIPTGLELEKFDPKNKNTELIEQIKDKYGIKDQFIVT
                     FLGRIAKEKSIEVLIDAMKEVIKENDNVLCLIVGGGPQLEELKELVKDDHISNYVIFT
                     GPKPSNEVPSYYHLSNVFVSASITETQGLTYIEAMASGIPAVARYDKNLEDVIDDGVN
                     GYFFKETSELVEILLKLINHNYSKMAEAAYLSAMKFSSEVFYEKVIAVYQQAINSKHY
                     SYTVKSIY"
BASE COUNT          557 a          198 c          305 g          540 t
ORIGIN      
        1 gcacgttcac ttgaaaactt tagaacagag ttaaatgtgt tattaaaaaa taagaatgta
       61 ttgataaaat catcattaat taatttgttt aaattattaa ttatgtatag tattcccttc
      121 tttgcagcaa aagcattaaa ccttaatgtt tcatttatcc aaatattcga ttttattggt
      181 atttgttcat ttgtttatat gattactgcc tttgttccga ttcctggagc aagtggcggg
      241 agcgaaggag tttattatat gttatttagc cctattttag gggctgttgg tacgccaaca
      301 acattattag tatggcgctt tgtaacctac tatttaggac taattattgg tggtatcatt
      361 tttgcaacta atcgagaaat aaatagatcg gagtaaagta atgagaatag caattttttc
      421 agatacatat atccctgata ttaatggggt agcgacatca actaaaatat tgaaagatga
      481 attagtaaaa catggacatg atgtagttgt tgttactagc gagttaccta gtgatagtga
      541 ttatatggat gatcctcatg ataatatctt aagagttcct ggattggaaa tacaagccct
      601 gtatggttac cgggcttgta atatttattc cttcaaagga atgcgtgaaa ttaagggaat
      661 gaacattgaa gttatccacg ttcaaactga atttggggta ggaatatttg gaagaatcgt
      721 aggagaaact cttaatattc ctgttgtcta tacgtatcat acgatgtggg cagattattc
      781 tcattatgtt aatcctgtaa attctggagc tattgatggt ttgatcaaaa aggcaattac
      841 acgtattagt aaattttatg gtgataaaag tactgaatta atcgtacctt ctttaaagac
      901 taaagaagca ttagaattat atggtttaaa taaagacgtc catattattc ctacaggatt
      961 agaactagaa aaatttgatc ctaaaaataa aaatactgaa ttgattgaac aaattaaaga
     1021 caaatatgga attaaagatc agtttattgt aactttttta ggacgaattg cgaaagaaaa
     1081 aagtattgaa gtattaattg atgcaatgaa ggaagttatt aaagaaaatg ataatgtact
     1141 ttgcttgatt gtcggtggag gaccacagtt agaggaattg aaagaattag taaaagatga
     1201 tcatatcagc aattatgtga tttttaccgg ccctaagcct agtaatgagg taccaagtta
     1261 ttatcattta tcaaatgtat ttgtttctgc ttcaataact gaaactcaag gattgactta
     1321 tattgaggcg atggcaagtg gaatacctgc tgttgcacgc tatgataaaa atttggagga
     1381 tgttatcgat gacggtgtaa atgggtactt ttttaaagaa acatcggaac tagtggaaat
     1441 cctactaaaa ttaattaatc ataattattc taaaatggca gaagccgcat acttaagtgc
     1501 aatgaagttt agtagtgaag tattttatga aaaggtaatt gctgtttatc agcaagcaat
     1561 taattcgaaa cactattcat atacggtcaa gtcgatatat
//
LOCUS       DAOMTA010000776         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4043_length_1598_cov_1.708360, whole genome shotgun sequence.
ACCESSION   DAOMTA010000776 DAOMTA010000000
VERSION     DAOMTA010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_1598_cov_1.708360"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..910
                     /gene="dapF"
                     /locus_tag="PLK68_15535"
     CDS             77..910
                     /gene="dapF"
                     /locus_tag="PLK68_15535"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRM91862.1"
                     /translation="MQLHFTKMEGIGNDYIYFDGINQEIPMDKEFIMKISDRHFGIGS
                     DGMIVILPSDQYDFKMRMFNLDGSEAMMCGNGIRCFAKFIYDHKLSDKHVLKIETKSG
                     LRTVELLFDGDECIGAKVNMGKPVLTCQDIPCTYAKEKMIDEPVEIGGREYRLTTISM
                     GNPHTVTFVSDLDTLDLKTIGPKFEHHQLFPASVNTEFVQIINKSYVKMRVWERGSGE
                     TMACGTGACAVMYACYLNHYTDSKVTVELLGGCLEIEYVDETIMMSGPATNVFNGTIE
                     I"
     gene            925..>1598
                     /gene="dapD"
                     /locus_tag="PLK68_15540"
     CDS             925..>1598
                     /gene="dapD"
                     /locus_tag="PLK68_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HRM91863.1"
                     /translation="MLNSAEEIIKYIGDAKKQTPVKVYLKGKNLPEATEFKMFGGEDS
                     KVCIGDLEAIKAYMEVNKEVIVDSYLEQDRRNSAIPMLDMTNINARIEPGCFIREHVT
                     IGDNAVIMMGAVINIGVKIGEGTMIDMGAVLGGRVEVGKRCHVGAGAVLAGVIEPPSA
                     SPVILEDDVLIGANAVVIEGVHIGKGAVVGAGSIVTSDVPAGAVVVGNPARIIKEQKD
                     ETTEGKT"
BASE COUNT          539 a          173 c          375 g          511 t
ORIGIN      
        1 ataaatataa tcttggaatt gaaaaatatt agtcaatttg ataaaatctt gtaaatacat
       61 gaaaaggagg ggaattatgc agctgcattt tacaaaaatg gaaggcattg gtaatgatta
      121 tatttacttt gatggaataa atcaggagat tcccatggat aaagaattta tcatgaaaat
      181 aagtgatcgt catttcggta ttggaagtga tggaatgatc gtgattttac catcggatca
      241 gtatgatttt aaaatgcgaa tgtttaatct agatggaagt gaagcaatga tgtgtggaaa
      301 tggaatccgc tgttttgcta aatttatcta tgatcataaa ttgagtgata aacatgtatt
      361 gaaaattgaa actaaatcag gattacgaac agtagaatta ttatttgatg gcgatgagtg
      421 tattggagca aaggttaata tgggcaaacc cgtcctaact tgtcaagata ttccttgtac
      481 atatgctaaa gagaaaatga ttgatgaacc ggtagaaatt ggtgggcgag aataccgttt
      541 aactactatt tcaatgggga atcctcatac tgtgactttt gtttctgatt tagatacttt
      601 ggatttaaaa acaataggtc ctaaatttga gcaccatcaa ttgtttcctg catctgttaa
      661 tactgaattt gttcaaatca ttaacaaaag ttatgttaag atgcgtgttt gggaacgcgg
      721 ttcgggtgaa actatggctt gtggtactgg tgcttgtgca gttatgtatg catgctattt
      781 aaatcattat acggatagta aggtaacagt ggaattatta ggtggttgtt tggaaattga
      841 atatgtagat gaaacgatca tgatgtctgg acctgcaaca aatgttttta atggaacaat
      901 agagatatag aaaaggagat agatatgtta aatagtgcag aagagataat taaatatatt
      961 ggtgatgcta aaaaacaaac accggtaaaa gtttatttaa aagggaaaaa cttaccagaa
     1021 gcaactgaat ttaaaatgtt tggtggagaa gatagtaaag tatgtattgg tgatttagag
     1081 gctattaaag cttatatgga ggtaaataaa gaggttattg ttgattctta tttagaacaa
     1141 gatcgtcgta atagtgcaat tccaatgtta gatatgacta atattaatgc tcgtattgaa
     1201 cctggatgtt ttattcgcga acatgtaacg attggcgata atgctgtaat tatgatgggt
     1261 gcagttataa atattggagt caagattggt gaaggaacta tgatcgatat gggagctgtt
     1321 ttaggtggtc gtgtagaagt tgggaaacgt tgtcatgttg gtgctggagc agttttagct
     1381 ggagttattg aaccacctag tgcttcacca gtaatattag aagatgatgt attgattgga
     1441 gctaatgctg tagttattga aggagtacac attggtaaag gtgcagttgt tggtgctggt
     1501 tcgattgtta caagtgatgt tccggcaggg gctgtggtag ttggaaatcc agctcgaatt
     1561 attaaagaac aaaaagatga aacaactgaa ggtaaaac
//
LOCUS       DAOMTA010000777         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4044_length_1598_cov_1.666235, whole genome shotgun sequence.
ACCESSION   DAOMTA010000777 DAOMTA010000000
VERSION     DAOMTA010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4044_length_1598_cov_1.666235"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="PLK68_15545"
     CDS             complement(<1..1572)
                     /locus_tag="PLK68_15545"
                     /inference="COORDINATES: protein motif:HMM:NF000326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HRM91864.1"
                     /translation="MIYFSFRFLLCNAIICIFLGSLLGLKNLLQRQLSARMQYNLSII
                     FLAVLIVPFLPINSAPSSISWRHLLTASSSTNGDIQTTFLSGNGYNLDKINDFAVSVS
                     TQIPTFIHTLLVFFWSIGIFIMFFLLYRSVKQVKALHSSALPLQNEELNALYIECLNE
                     VNSKHTIPIYSTAFLKSPVLAGFLHPRIYLPIHLISDFNAGTISATDIRYMLLHELQH
                     YKHKDILIGYLINTVNVFYWFNPLIWYFLKRIRQERELACDSAVLQLLKETEYKSYGN
                     TLINFAETIALSPFPLTMGISGNIKQLKGRILNIASFHQPTFKQKIRGYLICIFVSTI
                     IIGCIPILSAYASDQTGYHFDTTEKNITQLNLSSNFGDYTGSFVLYDQSADKWNIYNM
                     EHASTRVSPNSTYKIYDALLGLESGIITPEHSTFTWNGEPYPFNSWEADQDLTSAIHN
                     SVNWYFQAIDSQAGFEAVRTFLQTINYGNQNTGTNLNLYWTDFSLKISPIEQVELLQD
                     FYQNNFHFDSKNIQAV"
BASE COUNT          549 a          248 c          301 g          500 t
ORIGIN      
        1 tactgcctgg atatttttac tgtcaaaatg aaaattattt tgatagaaat cctgtaacaa
       61 ttccacctgt tctatgggtg atatcttcag agagaaatct gtccaataga gatttagatt
      121 tgttccagta ttctgattcc catagtttat tgtctgtaaa aatgttctta cggcttcaaa
      181 acctgcctgt gaatcaattg cctgaaaata ccagtttacg gaattatgta tagccgaggt
      241 taaatcttga tcggcttccc atgaattaaa aggatatggt tctccattcc atgtgaaagt
      301 ggaatgttct ggtgtaataa ttccagattc caagccaagt aatgcatcat atattttata
      361 agtcgaattc ggtgatacac gtgtggaagc atgctccata ttataaatat tccatttatc
      421 agcagactga tcatataaaa caaaacttcc agtgtagtcc ccaaaattgg aagaaagatt
      481 tagctgagta atattctttt cggttgtgtc aaaatgatac ccagtctgat cagaagcata
      541 agcagaaagt atgggaatgc atccaatgat gatagtagag acgaatatgc atataagata
      601 tccacgaatt ttttgtttaa aagtaggttg gtggaatgat gcaatattta aaatgcgtcc
      661 ttttaactgt tttatatttc cactaattcc catagtaaga gggaatggag acagagctat
      721 tgtttcggca aagttaatca gtgtatttcc atatgacttg tattctgttt ctttcaataa
      781 ttgtaataca gcactgtcgc aggcaagttc ccgttcctgt cgtattcttt ttagaaaata
      841 ccagattagg ggattgaacc agtagaaaac atttactgta tttatcaaat atccaattag
      901 aatatcttta tgcttgtagt gctgcagttc atgaagaagc atatagcgaa tatcagttgc
      961 gcttatagtt ccagcgttaa aatctgaaat caagtgaatt ggaagataaa tgcgaggatg
     1021 caggaaacca gccaaaacag gagatttcaa aaatgctgta ctataaatcg gaatggtatg
     1081 cttgctgttc acttcgttta aacattctat ataaagagcg tttagttctt cattttgaag
     1141 aggtaaagca gaactatgta aggcttttac ttgttttact gagcggtaga gaagaaaaaa
     1201 cattatgaat ataccgatac tccagaaaaa tacaagtaat gtgtgaataa aagttggtat
     1261 ttgggtgctg acagagacag caaaatcatt tattttatcc aaattataac cattaccaga
     1321 aagaaaagtg gtttgaatgt caccattggt acttgaactg gctgtcagca aatgtctcca
     1381 cgatatagaa gatggggcag agtttatcgg taaaaaggga acaattagaa cggcaagaaa
     1441 tataatcgaa agattatatt gcatacgtgc ggataattgc ctttgcaata aattttttaa
     1501 tcccagtaaa ctacctaaaa aaatacaaat gatggcatta cagagtaaaa atcgaaaact
     1561 aaaatatatc aaaatatgct cctcccatgg ttaatgat
//
LOCUS       DAOMTA010000778         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4054_length_1596_cov_2.341986, whole genome shotgun sequence.
ACCESSION   DAOMTA010000778 DAOMTA010000000
VERSION     DAOMTA010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_1596_cov_2.341986"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..901
                     /locus_tag="PLK68_15550"
     CDS             8..901
                     /locus_tag="PLK68_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM91865.1"
                     /translation="MKQLKEIKNYLVFHNGTIECHPENVGIAINDGKIDVKDAASLQI
                     IYLIDQEGKYCFDLKIKGQLELIETYDFKVPASLVKNIEVLQNSNILRYNDNQSSVNG
                     TVEVTENVSVERDSNVRCAYVELSDSSIEMNVNHALNGSNANSTVRLASLAKGQEKKH
                     MTFSLTHFAPHTTGIMDNYGVVKDASNLVIDGIGTIKQGNHQSSSHQTNKIIVFDEKC
                     NAKANPYLYIDEYDVKASHGASVGKIDEDHLYYLQSRGLSKKDAMHLVTYGYFIPVME
                     FIDNADLREMFNETLKEKVGI"
     gene            901..>1596
                     /locus_tag="PLK68_15555"
     CDS             901..>1596
                     /locus_tag="PLK68_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM91866.1"
                     /translation="MLDVEKIRQDFPMLNGATMHGQPLIYFDNGATTLKPQCVIDAVC
                     NYLTNYSGNAHRGDYDLSHDVDTQFEKTRDLVAKLINCDRKEVVYTYGSTDGLNMIAF
                     GYGMTHLDEGDEILLTVAEHASNTLPWFEVCDTVGSTVKYIDLDDEGRLTVANVLKAI
                     SDKTKIISIAQVSNVLGFDGPIKEICQIAHERGIIVCVDGAQSIPHEKVDVQTLDIDF
                     LVFSGHKMCGPTGV"
BASE COUNT          552 a          199 c          327 g          518 t
ORIGIN      
        1 ctaaataatg aaacagttaa aagagattaa aaattattta gtatttcata atggtacaat
       61 tgaatgtcac ccagaaaatg taggaattgc tattaatgat ggtaaaatcg atgttaaaga
      121 tgctgcctca ttacaaatta tttatctaat tgatcaagaa ggaaaatatt gttttgacct
      181 aaagattaaa ggacaactgg aattaattga gacttatgat tttaaagttc cagcatcttt
      241 agttaaaaac attgaagtat tgcaaaatag taatatttta agatataacg ataatcaaag
      301 cagtgtcaac gggactgttg aagtaactga aaatgtatcg gttgagcgtg atagtaatgt
      361 aagatgcgct tatgttgagt tatcagattc aagtattgaa atgaatgtta atcatgcttt
      421 gaatggttct aacgctaata gtacggtgcg cttagcttca ttagctaaag gacaggaaaa
      481 gaagcatatg acattttcgt taacgcattt tgcaccacat acaaccggga ttatggataa
      541 ttatggtgtt gttaaggatg catctaattt agttatcgat gggattggaa caattaaaca
      601 aggaaatcat caatctagca gtcatcaaac aaataaaatt attgtttttg atgagaaatg
      661 taatgccaag gcaaatccat atttatatat tgatgaatat gatgtaaaag caagtcatgg
      721 cgctagtgtc ggtaagattg atgaggatca tttgtattat ctccaatctc gtggtttaag
      781 taaaaaagat gcaatgcatt tagtaactta tggatatttt attccagtca tggaatttat
      841 agataatgct gatttaagag agatgtttaa cgaaacatta aaagaaaagg tggggattta
      901 atgttagatg tagaaaaaat tcgtcaggat tttcccatgt taaatggtgc tacgatgcat
      961 ggtcaaccac tgatttattt tgataatggc gctacaactt taaagcctca gtgtgtaatt
     1021 gatgctgtat gtaattattt gacaaactat tcgggtaatg cacatcgtgg tgactatgac
     1081 ttatcgcatg atgttgatac acagttcgaa aagacacgtg atttagttgc aaaattaata
     1141 aattgcgatc gaaaagaagt agtttataca tatggttcaa cagacgggct aaatatgata
     1201 gcatttggct atgggatgac gcatctagat gaaggtgatg aaatactttt aaccgttgct
     1261 gagcatgcct caaatacttt accatggttt gaagtctgtg atacggtggg aagtacggtt
     1321 aaatatattg atttagatga tgaaggacgt cttactgttg ctaatgtttt aaaagcaatt
     1381 agtgataaaa caaaaattat ttcaatagct caagtttcaa atgttcttgg atttgatgga
     1441 ccaattaaag aaatttgtca aattgctcat gagcgcggta ttatcgtttg tgttgatggt
     1501 gcgcaaagta ttcctcatga aaaggttgac gtccaaacat tagatattga ttttctagtt
     1561 ttttctggac ataaaatgtg cggaccaact ggggtg
//
LOCUS       DAOMTA010000779         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4081_length_1591_cov_2.082031, whole genome shotgun sequence.
ACCESSION   DAOMTA010000779 DAOMTA010000000
VERSION     DAOMTA010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_1591_cov_2.082031"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1591
                     /locus_tag="PLK68_15560"
     CDS             <1..>1591
                     /locus_tag="PLK68_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRM91867.1"
                     /translation="VNKNIPGGILAVQAHSGFPLYCFNQAILEYTQYSYEELCQITNG
                     KFSNIIYEKDRQFVEREIFQQLERNDIYEIRYRIVCHGGHLLWVYERGKYVIDGHGQK
                     LILSFFVDISHEMNNEQELRFINENSLDGVFKAALIEHFPILYANDGYYRIHGYTKKQ
                     FHHDLNDCAENLIYESDKARIRAQITDLIKNNQSQAILEYRIKKRDNSIAWIHISASF
                     TSLLDQTIIMIGMVMDITKRKKLEDKLYRSQQLFKVAQTKTGLNIWEFDIQNKQVIGN
                     PDSAVIRSYQNLTANIPESIIAQGLIHPQSINELKNLYQRLEKGAAPLSATIRVKQKD
                     AKDKYLWEKITYTIVQHIDEKPAWAIGISEDVTEQKEIETRIYNEESLRKVLNEDIIL
                     NIRLNFSRDFIEDISLHSNEQYEKQLSDYTYDDIYKYLLQTIANEDDQKKFEKKYSKN
                     QINDYIRTFTDIPTFEFRQKYLNGMILWVSLNMKTTISPQNNDVILFIYTKNIDIQKR
                     RELALQNKAEIDEMSSLYNEST"
BASE COUNT          617 a          236 c          248 g          490 t
ORIGIN      
        1 tgtaaataaa aatatcccgg gaggaatact agctgtccaa gcgcactctg gctttccact
       61 ctattgcttt aatcaagcca ttttagaata tacccaatat tcttatgaag aattatgtca
      121 aataacaaat ggaaaatttt caaatattat ctatgaaaaa gatcgtcaat ttgttgaaag
      181 agaaattttc caacagcttg aaagaaatga catttatgaa attcgttatc gcatagtttg
      241 ccacggaggc catttgcttt gggtatatga aagaggaaaa tacgttattg atgggcatgg
      301 tcaaaaatta attttgtctt tttttgttga tatttcccat gaaatgaata atgaacaaga
      361 actacgtttc attaatgaaa atagccttga cggagttttt aaagctgcat taattgaaca
      421 ttttccaatt ttatatgcta atgacggata ttatcgaata catgggtata ccaaaaaaca
      481 atttcaccat gatttaaatg attgtgctga aaatctcatt tatgaatcag ataaagcaag
      541 aataagagcc caaataacgg atttaattaa aaataatcag tcacaagcta ttcttgaata
      601 tcgaattaaa aaacgagaca attcaattgc ctggattcat attagtgcca gctttacttc
      661 gctgcttgat caaacaatta ttatgattgg aatggtcatg gatattacta aacgaaaaaa
      721 actagaagat aagttatatc gctcacagca gttgtttaaa gttgcacaga ctaaaaccgg
      781 cttaaatatt tgggagtttg atattcaaaa caaacaagta attggcaatc ccgattctgc
      841 agtaatccgc agctatcaaa atcttactgc caatatccca gaaagcataa ttgcacaagg
      901 gttgatccat cctcaaagca ttaatgaact taagaattta tatcaacgct tagaaaaagg
      961 agctgctccc ctttctgcta caatcagggt caaacaaaaa gatgcgaaag ataaatatct
     1021 ttgggaaaaa ataacatata ctattgtcca gcatattgat gaaaaaccag catgggcaat
     1081 tggcatatca gaagatgtta cagaacaaaa agaaattgag acaagaatct ataatgaaga
     1141 aagtttaagg aaagttttga atgaagatat tatattaaac atccgtttaa atttttctcg
     1201 tgattttatc gaagatattt ctttacattc caatgaacag tatgaaaaac agctctctga
     1261 ttacacatac gatgatattt ataaatattt attacagact attgctaatg aggatgatca
     1321 aaaaaaattt gagaaaaaat attctaaaaa tcaaattaat gactatatcc gtacttttac
     1381 cgatattcca acttttgaat ttaggcagaa atatttaaat ggaatgatat tatgggtatc
     1441 cttaaatatg aaaacaacca tttctcctca aaataacgat gtaatattat ttatttatac
     1501 caaaaatatt gatatccaaa aaagacgtga attagcattg caaaataaag cagaaattga
     1561 tgagatgagt agcttatata atgagtctac t
//
LOCUS       DAOMTA010000780         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4084_length_1590_cov_2.874919, whole genome shotgun sequence.
ACCESSION   DAOMTA010000780 DAOMTA010000000
VERSION     DAOMTA010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_1590_cov_2.874919"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLK68_15565"
     CDS             <1..616
                     /locus_tag="PLK68_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91868.1"
                     /translation="LSRISSLLKDEPVDENSTYQDIVKVIPTIYDKSIVQTRLDLDQN
                     KMIQVINRLSQAKRISIYGTGISYTIASQAAFKIMTLGKECDAHSGINEHFILSQKNS
                     RQCVAILISFTGNNSEMIKIAKYLKKAGIYVIAIGGKKGELQNYCDIYLNVYSSQDIL
                     SLEVITSFTATMYVLDVIFVSLLVKDYQSNVTTALKVIDFENKK"
     gene            968..>1590
                     /locus_tag="PLK68_15570"
     CDS             968..>1590
                     /locus_tag="PLK68_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91869.1"
                     /translation="MSLLSKLEYKKGFSDIEKGIANYIIDHKEEVANMRLVELAEATF
                     TSTATISRFCKKLGEKNYNSFKINFASSVLTSYQTDVDYNRPFKENDSIQEVTNQLGE
                     LYKDTIEATKALLDYDVLNQVIEKLLKTSVIDIFAVGASYLSGLLFEHRMISIDHFVN
                     FKSSPNDQDKRSLFVNKNTVAIVISYSGESHEIKTIVDRIVDNKGTI"
BASE COUNT          583 a          189 c          270 g          548 t
ORIGIN      
        1 attaagtcga attagtagtc ttttaaaaga tgaaccggtt gatgaaaaca gtacatatca
       61 agatattgtt aaagttatcc caactattta tgataaatca atagtacaga caaggctgga
      121 tttagatcaa aataaaatga ttcaggtgat taatcgttta tctcaagcta aacgtatttc
      181 tatttatgga acaggaatta gttatacgat tgccagtcaa gctgctttta aaatcatgac
      241 tttaggaaaa gagtgcgatg cacatagtgg aatcaacgaa catttcattt tatcacaaaa
      301 aaatagtcgt caatgtgtag ccatacttat ttcttttact ggcaataata gtgagatgat
      361 caagattgct aaatatttaa aaaaggcagg aatctatgtt attgcaattg gtggtaaaaa
      421 gggtgaatta caaaactatt gtgatattta cctcaatgtt tactcgtcac aagatatcct
      481 tagtttagaa gtgattacat catttactgc aacaatgtat gttctagatg tcatttttgt
      541 gtctttactt gtaaaggatt atcaaagtaa tgttactact gctttaaaag taattgactt
      601 tgaaaataaa aaataaatta tgtaaaaaat tttcataatt taatatttaa aattgctaat
      661 tttgaaaata ctatcctgaa ttatataaat ttatattttt ttataaaaca atggagtatt
      721 atatttccac aggctagttg gcttgtagaa atagtcgcta ctctcataag atatctattg
      781 acagttcata ttttaattta gtgactattt aatctaaatg atgaaaagtc caaaagggct
      841 tttttatttt agaaaaatca aaaaagaata gtgttaaaat agtgttaaaa aagactaaca
      901 tgcccttatt agtgttataa tggaaaaaat ttgggacaat atttataaag taattcgggg
      961 gtagtttatg agtttattat caaaactgga atacaaaaaa ggatttagtg acattgaaaa
     1021 agggattgct aattatataa ttgatcataa agaagaagtg gctaatatgc gtttagttga
     1081 gttagctgag gcgactttta cttcaacagc gacgatcagt cgtttttgca agaaactggg
     1141 tgaaaaaaac tacaatagct ttaagattaa ttttgcttca tcagttttaa ctagttatca
     1201 aactgatgtt gattacaatc gaccttttaa agagaatgat agtattcaag aagtcacaaa
     1261 tcagttaggc gagttatata aagatacgat tgaggcgact aaagcattac tcgactatga
     1321 tgtacttaat caagtaattg aaaagttatt aaaaacatca gttattgata tttttgctgt
     1381 tggagcttcg tatctttcgg gactattatt cgagcatcgg atgatttcga tcgatcactt
     1441 tgttaatttt aaatcttcac caaatgatca agataagcgt tctttatttg taaataaaaa
     1501 tacagttgca atcgtgatat cttattctgg tgagagtcat gaaataaaaa cgatagttga
     1561 tcggattgtg gataataagg gaactattat
//
LOCUS       DAOMTA010000781         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4088_length_1590_cov_1.536156, whole genome shotgun sequence.
ACCESSION   DAOMTA010000781 DAOMTA010000000
VERSION     DAOMTA010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4088_length_1590_cov_1.536156"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>452
                     /locus_tag="PLK68_15575"
     CDS             <1..>452
                     /locus_tag="PLK68_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRM91870.1"
                     /translation="KLNAGEKIIGTILVKDFNLPLYVMLATKNGQIKRTNLKDFEVTR
                     YSKPLKCMGLKNDDRVVDVKLTDNDQGIILTTKAGYGALYSEQEVSIVGIKAAGIRAV
                     NLKNDELVSLNIFNPLVSSSLIVISEEGGVKRIKIGDITPCNRATKGT"
     assembly_gap    453..462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <463..870
                     /locus_tag="PLK68_15580"
     CDS             <463..870
                     /locus_tag="PLK68_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM91871.1"
                     /translation="KSKIIYVKDSVIVDNSQLITVTLNDQTSFEFKATDYNNANLEQK
                     PSVANKLTANQVIDDVYINSPISTSDYDVAKKTEVDLEIFKQPKEHTLLDDFESDEVE
                     TTKNKAVVKVPKKTEPIKYEKISLEDILKEDDF"
     gene            complement(888..1558)
                     /gene="sigK"
                     /locus_tag="PLK68_15585"
                     /pseudo
     CDS             complement(888..1558)
                     /gene="sigK"
                     /locus_tag="PLK68_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
BASE COUNT          568 a          227 c          238 g          547 t
ORIGIN      
        1 aaaactaaat gctggtgaaa aaatcatcgg aactattttg gtaaaagatt ttaatttacc
       61 attgtacgtt atgctagcaa caaaaaatgg tcaaatcaaa cggacgaatt taaaagattt
      121 tgaagttact cgatattcaa agccccttaa atgtatggga ttaaagaatg atgatcgggt
      181 agtcgatgtt aaattgactg ataatgatca aggaattatc ttaacgacta aagctgggta
      241 tggagcatta tattcagagc aagaagtttc aatcgttgga atcaaagccg ctgggattag
      301 agctgtaaat ttaaaaaatg atgagttagt tagtctcaat atttttaatc cattagttag
      361 cagttcatta atcgttatta gcgaagaagg tggggttaaa cgaattaaaa ttggcgatat
      421 aacaccttgc aatcgtgcta caaaaggaac ttnnnnnnnn nnaaatctaa aattatttat
      481 gtaaaagatt ccgtaattgt tgataattca caattaatta cagtcacttt aaacgatcag
      541 acttcatttg aatttaaggc aactgactat aataatgcta atcttgagca aaaaccaagt
      601 gtagctaata aattaacggc taatcaagta attgatgatg tttatataaa ttctccaatt
      661 tcaacaagtg attatgatgt agctaagaaa accgaagttg atttagaaat ctttaaacaa
      721 cccaaagagc ataccttatt agatgatttt gaaagtgatg aggttgaaac taccaaaaat
      781 aaagcggttg ttaaagtacc caaaaaaaca gaaccaatta aatatgaaaa aatatctcta
      841 gaagatattc taaaagaaga tgatttttaa accgtaaatt atataattta cggtttttct
      901 ttcatatgtt cacgcaataa tttaattaat gcacgttttt caagacgtga aacataagaa
      961 cgtgagatat ttaatttttt agcaatatct ttttgagtat actttttggt attattcaag
     1021 ccataacgca taatcaaagt atctttttcg cggggttcaa gaacactaaa atatgttgta
     1081 aatttatcta aagtatcctt tttatctact atatcgataa actcctcttg ttttgcggca
     1141 atgatatcac ctaaaactat ttcactgcca ttgtctcatt taatgaaata tctaagttag
     1201 tctttttatt agttctaaga tgcattaata tctcattttc aatacatctg gaagcataag
     1261 tggctaactt agttttatgg ttttgtttat aactatcgac tgctttaatc agtcctattg
     1321 ttccaattga aatcagatct tcctgcatat ctttattgtt ctcgtatttt ttagcaatat
     1381 gagctaccaa tcttaaattt ctttcaatta aaacattccg agcctctttg tcaccattaa
     1441 aaaactgttc aagatatctt tcttcctctt tagctgttag tggtaactca aaagattttg
     1501 atttaatgta tgagacaaag aaaccattag taagtaagga taataatgtt gaaaacattt
     1561 atatttcacc tctaatacat atatattgtc
//
LOCUS       DAOMTA010000782         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4089_length_1589_cov_2.368318, whole genome shotgun sequence.
ACCESSION   DAOMTA010000782 DAOMTA010000000
VERSION     DAOMTA010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_1589_cov_2.368318"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /gene="gpmI"
                     /locus_tag="PLK68_15590"
     CDS             complement(<1..1026)
                     /gene="gpmI"
                     /locus_tag="PLK68_15590"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRM91872.1"
                     /translation="MKKRPIVLCILDGYGLSERIDGNAVKLANTPNIDDLEMIYPTTR
                     IKASGMPVGLPDGQMGNSEVGHLNIGAGRTVYQSLTLINKAVEDGTFYKNAEFLKAIN
                     NAKENNTKLHIWGLLSNGGVHSSNEHIYALLKLAKQEGLEKVYVHAFLDGRDVAPDSG
                     ADFVKELADKIEEIGVGEIATVSGRYYAMDRDKRFDRVELAFDAIVNHKGESFECPVQ
                     YVKDSYAKETYDEFVIPGYNKNVDGQVADGDSVIFANFRPDRAIQLATVMTNDGFYDH
                     AFNNVPKNLTFVCMMKYADSVNGAIAFALPSLTNTLGDYLSAKGMKQLRIAETEKYAH
                     VTFFFDGG"
     gene            1229..>1589
                     /locus_tag="PLK68_15595"
     CDS             1229..>1589
                     /locus_tag="PLK68_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRM91873.1"
                     /translation="MKKLLCLLLVFMLCGCSIDKRATTKTVDVSPYLYKISDNNNHYL
                     YLLGTCHPGRDQIDKLDITTEKAIKDSQSIVLECSLDQNETAKYQNYLLENSLGELDL
                     INYIPKLKENYPTLKKYR"
BASE COUNT          565 a          276 c          183 g          565 t
ORIGIN      
        1 accaccgtca aagaagaaag taacatgagc atatttttca gtttctgcaa ttcttaattg
       61 tttcatacct tttgcagata aataatcacc aagtgtatta gttaaacttg gtaaagcaaa
      121 agcaatcgca ccattaacac tatctgcata tttcatcata caaacaaatg ttaaattttt
      181 tggcacatta ttaaatgcat ggtcatagaa tccatcatta gtcatcactg ttgctaattg
      241 aatcgcacgg tcaggtctaa agtttgcaaa gataactgaa tcaccatcag ctacttgtcc
      301 atcaacattt ttattatatc caggaattac aaattcatca taagtttctt tagcatatga
      361 atctttaaca tattgaacag gacattcaaa gctttcacct ttatgattaa cgatagcatc
      421 aaacgctaat tcaacacgat caaacctctt atcacgatcc attgcgtaat atctaccaga
      481 tacagttgca atttcaccaa cacctatttc ttcaatttta tctgctaatt ctttaacaaa
      541 atcagcacca ctatcaggag caacgtcacg accatctaaa aatgcgtgta catatacttt
      601 ttctaaacct tcttgtttag ctaattttaa taaggcatag atatgttcat ttgaagaatg
      661 cactccacca tttgataata atccccaaat atgtaattta gtattatttt ctttggcatt
      721 attaattgct tttaagaatt cagcattctt atagaatgtt ccatcttcaa cagctttatt
      781 gattaaagtt aatgattggt aaactgttct tccagctcca atatttaaat gtccaacttc
      841 actattaccc atttgtccat caggtaatcc gacaggcatt ccagaagctt taattcttgt
      901 tgttggataa atcatttcta aatcatctat atttggtgta tttgcaagtt taactgcatt
      961 tccatcaatt ctttcactta atccgtatcc atctaaaata cataatacaa ttggtctttt
     1021 tttcattatc taattcctcc attttcatga accattataa cttgtttaac tacaataatc
     1081 aaacaagaaa tacttgaaat aatctaaatt taataacttt ttactaacaa aatctcttca
     1141 gttaaaatag ttttatattt ctataatata ttgatatata aataatatag ttatataatt
     1201 ataatataaa taataaaagg gaaatgctat gaaaaaatta ttatgtttat tactcgtttt
     1261 tatgctgtgt ggttgtagta ttgataaaag agcaactact aaaactgttg atgtcagtcc
     1321 atatctctat aaaattagtg acaataataa tcactatctt tatctattag gaacctgtca
     1381 tcctggtcgt gatcaaattg ataaacttga tataactacc gaaaaagcaa tcaaggatag
     1441 tcaaagtatt gttttagaat gttcattaga tcaaaatgaa actgctaaat atcaaaatta
     1501 tcttttagaa aacagtttag gtgaactaga tttgattaac tatattccta aattaaaaga
     1561 aaactatcct acactaaaaa aataccgtg
//
LOCUS       DAOMTA010000783         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4093_length_1588_cov_2.304631, whole genome shotgun sequence.
ACCESSION   DAOMTA010000783 DAOMTA010000000
VERSION     DAOMTA010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4093_length_1588_cov_2.304631"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..559
                     /locus_tag="PLK68_15600"
     CDS             146..559
                     /locus_tag="PLK68_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91874.1"
                     /translation="MVDRNDYKIIINKGKTKEKFFLFLTVGEMGFCLFIGVLSFKFFG
                     MFMSELYSLLIAVVLSASIASLFIEIPVSHLSVLHHLKLACKYYFTLPNQYYYYRDKN
                     KEENINKEEENSDFYQEERYTASKKTKKADKRNHR"
     gene            486..>1588
                     /locus_tag="PLK68_15605"
     CDS             486..>1588
                     /locus_tag="PLK68_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91875.1"
                     /translation="MIFTKKKGTQQVKKQKKQTKETTGRTRHIKYAIPVTWNEDRQCF
                     IDNEEHFILMAKTDGTNLFGLKEEDQNVYMNAFASIFNSNTGSGQIYSYEVPADVDGY
                     VNDYNYLKSQLDVVGSEIDQIKYKILDDASIRLEDTSVTRELVDRCFVIILKDKDWFK
                     LERRLADVISYLNSYQRTRLMTPQEMLEVVYNYYNPANGKFVPSVYKDQFGVMECIYP
                     DYIGFYDKGFNQSIVLNDLYCKTKWLATFYKEPVMALLCYLATARGVDFSLHWSPAPH
                     DAITKDIDKSIRALEKRANNEKDLSKVAKSQKEINENIEMVEKLINDGNFPIYFSVSI
                     RVVASSPDLLEESVKAIDKDVKQFNIKFRDGIH"
BASE COUNT          568 a          189 c          300 g          531 t
ORIGIN      
        1 ggaattatac taatggttct tgtctgcctg gcagttttgt tctgtacatg tgtaataatt
       61 cttatcatgt acggatatct gcattttatg ctaggtattt tactatatta aaatcaggtg
      121 atgataattt ataggaggtg tatagatggt tgatcgaaat gattacaaaa taattatcaa
      181 taaaggaaaa actaaagaaa agttttttct ttttttaact gttggtgaga tgggattttg
      241 cctttttata ggtgttttga gttttaagtt ttttggaatg tttatgagtg aactttattc
      301 actgctgata gcagttgttt taagtgcgtc tatagcatcg ctttttatag aaataccagt
      361 ttcgcacctc agtgttctac atcatttaaa actggcatgc aagtattatt ttactttacc
      421 aaatcagtat tactattatc gtgataaaaa caaagaagag aacattaata aggaggaaga
      481 aaatagtgat ttttaccaag aagaaaggta cacagcaagt aaaaaaacaa aaaaagcaga
      541 caaaagaaac cacaggtaga acacgtcata taaaatatgc aattcctgtt acatggaatg
      601 aagacaggca atgttttatt gataatgagg aacattttat tttaatggca aaaacagatg
      661 gtacgaacct gtttggatta aaagaagaag atcaaaatgt atatatgaat gcatttgctt
      721 cgatttttaa ttctaatact ggttcaggac aaatttattc ttatgaagtt cctgcagacg
      781 ttgatggtta tgtaaatgat tacaattatt taaaatctca actggatgtt gttggaagcg
      841 agatagatca aataaaatat aagattttgg atgatgcttc tataagatta gaagatacaa
      901 gtgttactag agaactggta gatcgatgtt ttgtgattat attaaaagat aaagactggt
      961 tcaagctcga aagaagattg gcagatgtga tttcgtatct aaattcatat caaagaacca
     1021 gacttatgac accgcaggaa atgttagaag tggtatataa ttactataat cctgcaaatg
     1081 gaaagtttgt tccttcagtt tataaagacc agtttggtgt aatggaatgt atatatccag
     1141 attatattgg tttttacgat aagggcttta atcagtcaat tgtattaaat gatttgtatt
     1201 gtaagactaa atggttagca acgttttata aagagcctgt aatggctctt ttgtgttatc
     1261 tggctactgc cagaggagtt gatttttcac ttcactggtc acctgcacct catgatgcta
     1321 ttacgaaaga tatagataaa tctattagag cgctagaaaa aagagcaaat aatgaaaagg
     1381 atttatcaaa agttgccaag agtcaaaagg aaatcaacga aaatattgaa atggtagaaa
     1441 aattaataaa tgatggaaat tttccaattt attttagcgt ttcaattaga gttgttgcca
     1501 gtagtcctga tttattagaa gaatccgtca aagcaattga taaagatgtt aaacaattca
     1561 atattaaatt tagagatggt atacacca
//
LOCUS       DAOMTA010000784         1581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4118_length_1581_cov_2.765400, whole genome shotgun sequence.
ACCESSION   DAOMTA010000784 DAOMTA010000000
VERSION     DAOMTA010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_1581_cov_2.765400"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1143)
                     /locus_tag="PLK68_15610"
     CDS             complement(112..1143)
                     /locus_tag="PLK68_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM91876.1"
                     /translation="MINNKLVLAYIGNGKSANRYHLPFVLQRSDKFIVKKIFDIRIRH
                     DLWKTIDGVEYVEDVNKILNDPKIDMVVICTAHHLHYDYAKMVLNAGKHCLVEKPFME
                     NSAQAKEIFALADEKGLYCSAYQNRRYDSDFLTVQKVIESGKLGDLLELEMHFDYYRP
                     EVPEKINHFDPAMSYLYGHGCHTLDQVISYFGKPDTINYDVRQLLGEGRFNDYFDLDM
                     YYGTLKVSVKSSYFRVKERPSFVVYGKKGMFIKQSKDRQEEHLKLFYMPTNKDFGVDT
                     PEHYGTLIYYDQDGFYHEEKVVSVTGDYARVYDGIYDCIVKHQPQIITHEQTLLQMEI
                     LETGISKLK"
     gene            complement(1179..>1581)
                     /locus_tag="PLK68_15615"
     CDS             complement(1179..>1581)
                     /locus_tag="PLK68_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRM91877.1"
                     /translation="FDNEDAFLLGCNLVEKVKKENLKNIRIRIVLNQDIVFQYLMNGK
                     KGDQWLNRKQNTVELFKLPTYQIWQENERSHCYQQYTNDERYVICGGAYPIIVGKGMI
                     GSVIVSGLAHNEDHQIIVDVLSKYHDEKNKI"
BASE COUNT          545 a          311 c          160 g          565 t
ORIGIN      
        1 tatgctcaac tgacttaagt ctatatgttc aaattttcaa tttgaacagg taaaaaaata
       61 aaaaagatta ctcgactgag taatctttac aacctaaaaa caaataaatt attattttaa
      121 ctttgaaata cctgtttcca atatttccat ttgtaataat gtttgttcat gagtaataat
      181 ctgaggctga tgcttaacaa tacaatcata gataccatca tacactcggg catagtcccc
      241 tgttacacta accacttttt cttcgtgata aaaaccatct tgatcataat agataagggt
      301 accataatgc tccggtgtat caactccaaa atctttattc gtcggcatat aaaataattt
      361 taaatgttct tcttgacgat ccttgctctg tttaataaac attcccttct taccatatac
      421 aacaaaacta ggtctttctt taactctaaa ataacttgat ttaacagaca ctttcaacgt
      481 tccataatac atatctagat caaaataatc attaaaacgc ccctctccta ataattgacg
      541 aacatcataa ttaattgtat caggttttcc aaaataacta ataacctgat ctaatgtatg
      601 gcaaccatgc ccatataaat atgacattgc gggatcaaaa tgattgattt tttctggaac
      661 ctccggacga taataatcaa aatgcatttc caattctaat aaatctccta atttcccact
      721 ctctattact ttttgtaccg ttaaaaaatc actgtcatag cgcctatttt gatatgctga
      781 gcaatacagc cctttctcat cagcaagagc aaaaatctct ttagcctgtg cactattttc
      841 catgaatggt ttctcaacta aacaatgctt tccagcattt aagaccattt ttgcataatc
      901 atagtgtaag tgatgagctg tacaaataac gaccatatct attttaggat cattcaatat
      961 cttattaaca tcctcaacat actcaactcc atcaatagtt ttccataaat catgtctaat
     1021 cctaatatca aatatctttt taacaataaa cttatctgat ctttgtaaga caaaaggtaa
     1081 atggtaacga ttagcacttt tgccattccc aatataagct aaaactaatt tattattaat
     1141 cattttcaga tactactcct ttctattatt cataatattt atattttatt tttctcatca
     1201 tgatattttg ataaaacatc aacaattatt tggtgatcct cattatgagc taatccagat
     1261 acaataacac ttccaatcat ccctttacca acaataatag gataagcacc accacatatc
     1321 acatatcttt catcattagt gtattgctga taacaatgac ttcgttcatt ctcttgccaa
     1381 atttgataag ttggcaattt aaatagttct actgtgtttt gtttacgatt taaccattga
     1441 tcaccttttt tgccattcat caaatattga aaaacaatat cttgatttaa aacaattctg
     1501 attctaatat tttttaagtt ttcttttttt actttttcaa ctaaattaca accaagcaaa
     1561 aaagcatctt cattatcaaa c
//
LOCUS       DAOMTA010000785         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4124_length_1580_cov_2.556721, whole genome shotgun sequence.
ACCESSION   DAOMTA010000785 DAOMTA010000000
VERSION     DAOMTA010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4124_length_1580_cov_2.556721"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    594..603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<604..1260)
                     /locus_tag="PLK68_15620"
     CDS             complement(<604..1260)
                     /locus_tag="PLK68_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM91878.1"
                     /translation="MDNKEIGIRILSARKNLGYTQKQLGNLINVSDKAVSKWERGIGC
                     PDISLLLPLSEALKMSIDELIGGTVVDKSEKKTVQNLINYTKIKAIENKERIIKISYL
                     FITIVMMIGIFVPCLCNYYLNHSFTWSIISSAAIIYAWIILTVLVQAKENVVIKAIIV
                     ASIGVIPLLYVVSVQVYSLDWFLHEALVIALFTNLYVYIVIWIWVKTTLNIWYKIGLC
                     I"
     gene            complement(1372..>1580)
                     /locus_tag="PLK68_15625"
     CDS             complement(1372..>1580)
                     /locus_tag="PLK68_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HRM91879.1"
                     /translation="IKYVHPIKKYNSQQRVVRIFDPDNHMIEIGEAMDVVITNLLLAK
                     KDIDEVSRITQYPIDYVRNIFNKL"
BASE COUNT          540 a          273 c          169 g          588 t
ORIGIN      
        1 caaaatgtta ttttgaccaa tcaacattgt cgataatgtt actaaacaca ggaccccttt
       61 aataattcga ttatttactt ttttcatata cttttcccct aattacatat ttgtacgtag
      121 tgtactaagt aaatttatca tagccgtcaa tttctaacta tttctatttt gtaaatttta
      181 aaataagatt ttgtaaataa aaaactattt ccagttagaa atagtctacc tttgttttca
      241 tagcatcatt gctacaaaaa tttgaattaa tcctgcaaag gcaagactaa aaccaattgt
      301 tctattaagt atcattatta gagtactaat catgatcaat attttcctaa taattattga
      361 tgctttggtc ttttacaggg gtccttcttt attcaaataa attaattgaa tcaagttttt
      421 tattgttttt gatatttttt ttcaattttt gatttataaa catcttaatt atgctttctc
      481 gtatagccaa ccataataat aattgttccg acaatcatat ttaaagctaa attcataatc
      541 atcgaaaata aattgacacc agatatgtag ttggcaggaa tattagtaat attnnnnnnn
      601 nnngatacat aaaccaatct tataccaaat attcagcgtt gtttttaccc atatccatat
      661 gactatatat acatacaaat tagtaaacaa agcaataacc aaagcttcat gtaaaaacca
      721 gtctaatgaa tacacctgaa ctgaaactac ataaagcagt ggaattaccc ctatactggc
      781 tacaataata gctttaataa caacattttc tttagcttga actaaaaccg ttaaaattat
      841 ccaagcataa ataattgctg cactacttat tattgaccac gtgaaactat gatttaggta
      901 ataattacat aaacatggta caaagattcc aatcatcatt acgattgtga taaaaagata
      961 actgatctta atgattctct ccttgttttc aattgctttt atctttgtat aattgattag
     1021 attttgaact gtctttttct cacttttatc aactacagta ccaccaatca attcatcaat
     1081 actcatcttt aaagcctcac ttaacggtaa taataatgag atatcgggac acccgattcc
     1141 acgttcccat tttgatacag ctttatcact tacattgatt aaatttccta attgtttttg
     1201 agtataccca agatttttac gagctgataa aattcgtatc ccaatttctt tattatccat
     1261 aaaattactt cccttctacg tcctaatcct aacataaaag ttataatttt actatcgacg
     1321 gactgtttac ctcactagaa tccttgatct ataaaaaaag ttgtagtcaa cctacaactt
     1381 attgaaaata ttccttacat agtcaatagg atattgtgta attctactta cttcatctat
     1441 atctttttta gccaataata gatttgtaat tactacatcc attgcttcac caatttcaat
     1501 catatgatta tcgggatcaa agattcgtac tacccgctgt tgcgaattat attttttgat
     1561 tgggtgaaca tatttaatat
//
LOCUS       DAOMTA010000786         1579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4133_length_1579_cov_1.345801, whole genome shotgun sequence.
ACCESSION   DAOMTA010000786 DAOMTA010000000
VERSION     DAOMTA010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_1579_cov_1.345801"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLK68_15630"
     CDS             <1..221
                     /locus_tag="PLK68_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM91880.1"
                     /translation="ETVGLGMNAKNDNFKNQYYHKSVTDFKITKTGKQNEDEIDALSG
                     ATITSSAFNNAINGALAINQEIKEANHE"
     gene            214..945
                     /locus_tag="PLK68_15635"
     CDS             214..945
                     /locus_tag="PLK68_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRM91881.1"
                     /translation="MNKYVERLYNGIIKENPTLILMLGMCPTLAVTTSSVNGLGMGLT
                     TMIVLAASNFMISFLRKIIPERMRIPSYIVIVASMVTIVQLLLQAYLPSLYDTLGIYI
                     PLIVVNCIILGRAEAYASKHSCVLSLFDGVGMGLGFTLALTCIGLIREMLGSGTFFGI
                     EFAPLFKDLVGTNIYSFFAHLEPVTIFVMAPGAFFVLAGLTAIQNRLKLPSATNKNKD
                     GNCNHDCLHCRSSDEKALCDLKEEK"
     gene            946..1518
                     /locus_tag="PLK68_15640"
     CDS             946..1518
                     /locus_tag="PLK68_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HRM91882.1"
                     /translation="MTGMILLLISVALVNNVVLSQFLGLCPFLGVSKKIETAAGMGGA
                     VIFVMSIASIATSLVYKILVLFHLEYLNTVTFILVIAALVQLVEMFLKKYSSGLYSSL
                     GVYLPLITTNCAVLGVAITNVQNNYDILTSLVCSFGTAVGFTIAIVIMAGIREKIADN
                     DIPVSFQGSPIVLITAGLMAIAFIGFSGLI"
BASE COUNT          484 a          241 c          298 g          556 t
ORIGIN      
        1 atgagacggt tggattagga atgaatgcta aaaatgataa ttttaaaaat caatattatc
       61 ataaatcagt aactgatttt aaaatcacca aaaccggtaa gcaaaatgaa gatgaaattg
      121 atgctctaag cggagctaca attacttcat cagcttttaa caatgccatt aacggagcct
      181 tagcaatcaa ccaggaaatc aaggaggcta atcatgaata aatatgttga acgtctttat
      241 aatggaatca taaaagaaaa tccaactttg attttaatgt taggaatgtg tcctactctt
      301 gcagtaacaa cttcttctgt caatggatta ggaatgggct taacaacgat gattgtcctt
      361 gccgcatcaa attttatgat ttcgttctta cgaaaaataa tccctgaaag aatgcgaatt
      421 ccttcttata tcgtaattgt cgcttcaatg gtaacaattg tccaattact attacaagca
      481 tatttgcctt cattatatga tacattaggg atctacattc ctctaattgt ggttaattgt
      541 atcattttag gacgagctga agcttacgct tcaaaacata gttgcgtttt atcgttattt
      601 gatggtgttg gtatggggct aggttttacg cttgcactga cttgtattgg tttaattaga
      661 gaaatgttgg gcagtggtac tttttttgga attgagtttg cgccactttt taaagattta
      721 gttggcacta atatttatag cttttttgct catcttgaac ctgtaactat ctttgtaatg
      781 gcacctggcg ctttctttgt tttagctggt ctaacggcta ttcaaaaccg tttaaaactg
      841 ccaagtgcaa ctaataaaaa caaagatggt aattgtaatc atgattgtct tcattgtcgt
      901 agcagtgatg aaaaggcact ttgtgattta aaggaggaaa aataaatgac gggaatgatt
      961 ttattattaa ttagtgtcgc tttagtaaat aatgttgtct taagtcaatt cttaggtcta
     1021 tgtccttttt taggagtatc aaaaaaaatt gaaacagcag caggaatggg tggtgctgtc
     1081 atctttgtaa tgtctattgc ttcaattgca acaagtttag tttacaaaat actagtactc
     1141 ttccatttag aatatttaaa cactgttacc ttcatcttag ttattgctgc tttggtgcag
     1201 ctagttgaaa tgttcttaaa aaaatatagc tccggcttat atagttcttt gggagtatat
     1261 cttcctctta tcacaactaa ctgtgccgtt ttaggagttg ctatcacaaa tgttcaaaat
     1321 aattatgata ttttgacaag tcttgtttgt agttttggaa cagctgttgg atttacgatt
     1381 gccatcgtta ttatggctgg gatacgtgaa aaaatcgctg ataatgatat tcctgtttca
     1441 ttccaaggtt cccccattgt actaatcaca gcaggactga tggcaattgc attcattggc
     1501 ttttcaggtc taatataagg aggagaatat aagatgaatg aaattataat tgcggccatt
     1561 attgtaggtg caatcggtt
//
LOCUS       DAOMTA010000787         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4135_length_1578_cov_2.617859, whole genome shotgun sequence.
ACCESSION   DAOMTA010000787 DAOMTA010000000
VERSION     DAOMTA010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_1578_cov_2.617859"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..816)
                     /locus_tag="PLK68_15645"
     CDS             complement(124..816)
                     /locus_tag="PLK68_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM91883.1"
                     /translation="MTNRIMQKEIVSDPFTALVPNTDITDTLQEFMALQQLYDAGIKE
                     VRTKLEILDDEFKIKHDHNPIHHMEYRLKSVNSILGKLEKRGLEVSLDSIVTNLTDIA
                     GVRVICNYVSDVYKIADLLIKQSDIKLIAKKDYIKHPKENGYRSLHLVVEVPIFLAEK
                     VQPTTVEIQIRTIAMDFWASLEHHLRYKADNEVPDGVRDELIECAKTISNLDYKMQGI
                     HEELNKPKKKVI"
     gene            890..1252
                     /locus_tag="PLK68_15650"
     CDS             890..1252
                     /locus_tag="PLK68_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91884.1"
                     /translation="MKKTIVVLLIMMNIVSVNASSNDPKYKIIANSNSDKDIKTMYET
                     KDDLIKDYKDWIKSVDDVDQALADHQKDYQAKYYNGEYTIVLGAGKGKELTGTLKASY
                     CVSSKEIKKKSFFAELFS"
     gene            1392..>1578
                     /locus_tag="PLK68_15655"
     CDS             1392..>1578
                     /locus_tag="PLK68_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM91885.1"
                     /translation="MKTVKDAEIFCQYIAHEYLGKRNYKLLEDIIDKRITIIGTGAHE
                     VSRNIQELMTALNKEAEL"
BASE COUNT          567 a          229 c          249 g          533 t
ORIGIN      
        1 gaatattata ataacgagag aatcaataca aaaagaaaag gattgagtcc acttgcatat
       61 agacaacaat cctttcaaga tttagcttta atttaatgtg tccaactttt tgggaatact
      121 acactaaatc acttttttct ttggtttatt taattcctca tggatacctt gcattttata
      181 atccagatta gaaattgttt tagcacattc aattagctca tcacgaactc catcaggaac
      241 ttcattatca gctttatatc gcaagtgatg ttctaaactt gcccaaaagt ccatcgcaat
      301 cgttctaatt tgaatttcta cagtagttgg ctgaactttc tctgctaaga agataggaac
      361 ttcaacaact agatgtaaac tacgataacc attctccttg ggatgtttaa tgtaatcttt
      421 tttggcaata agtttaatat cactttgttt tattaataaa tcagcaattt tatagacatc
      481 gctaacataa ttacaaataa cgcgaacacc agcaatatca gtcaaattag ttacaataga
      541 atctaaagat acttctaggc cccgtttttc tagttttccc aatatactgt ttacagattt
      601 taaacgatat tccatatgat gaataggatt atgatcatgt tttattttaa actcatcatc
      661 aagaatttct aattttgttc ttacttcttt aattcccgca tcataaagtt gttgcagcgc
      721 cataaattct tgtaaagtgt cagttatatc tgtattagga actagtgcgg taaaaggatc
      781 actgacaatt tccttttgca taattcgatt agtcataaaa taccccttta ttaaatattt
      841 tttgtataac ttaatcatag ttggtaataa tgaacttgag gtgaactaga tgaaaaaaac
      901 aattgtagta ttattaatta tgatgaatat tgtatcagta aatgcttcaa gtaatgaccc
      961 aaaatataaa ataattgcca atagtaatag tgataaagat attaagacaa tgtatgaaac
     1021 aaaagatgac ttaattaaag actataaaga ttggattaaa agtgttgatg atgttgatca
     1081 agcactagct gatcatcaaa aagattatca agctaagtat tataatggag aatatacaat
     1141 tgtcctaggc gctggcaaag gaaaggaact tacgggtact ttgaaggcaa gttattgtgt
     1201 aagtagtaaa gaaattaaaa agaaatcatt ttttgcagaa ttattttctt aagattatca
     1261 gtgaagcgaa tccttctagt tggttatacc caacttttgg aactcacttt actgatagtc
     1321 ttttattatg tcctaaatat aactgaaata tgctatacta tttaatagag tagtaatgaa
     1381 aggagtcaga gatgaaaaca gtaaaagatg ctgaaatctt ttgtcaatat atagcccatg
     1441 agtatcttgg gaagcgtaat tataagttat tagaagatat aattgataaa aggattacaa
     1501 tcattgggac gggagctcac gaggttagtc gcaacattca agaacttatg acggctttga
     1561 ataaagaagc agaacttt
//
LOCUS       DAOMTA010000788         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4140_length_1577_cov_2.223390, whole genome shotgun sequence.
ACCESSION   DAOMTA010000788 DAOMTA010000000
VERSION     DAOMTA010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4140_length_1577_cov_2.223390"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..1107
                     /locus_tag="PLK68_15660"
     CDS             544..1107
                     /locus_tag="PLK68_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91886.1"
                     /translation="MILDYPKIYKEKELIGQGGNQDWFADSWARKAGCASVSATDIFI
                     YYTKREREFDKSTYLDYMNEMFKLMEPGKRGFPYVFLYARRLSKLLNNCEYRIFRRPR
                     LYDASEIVKESIEHGNPLGLLILTHRRRKIRDDLWHWVTIVGYEETKKGLDIIFLDCG
                     DEKRIPARILFEKSRFNVVKMVRFFYR"
     gene            1248..1511
                     /locus_tag="PLK68_15665"
     CDS             1248..1511
                     /locus_tag="PLK68_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRM91887.1"
                     /translation="MKLLISYIIIINLMMILLYGIDKYKAIHHHWRVSEKILLIGALF
                     GGSLGAILAMYGFNHKTRKNIFKYGIPLLLGLQIILIIKISIG"
BASE COUNT          539 a          170 c          301 g          567 t
ORIGIN      
        1 aagcgataaa tagagaaatt ttcagcttca attttttcat caagaactaa tttttttggt
       61 aaataatgaa ttttatgaat ataaggctca ctgccaagac agtaagttag ctcaattttt
      121 aaacatttag aatcataagc tgaatataat tcagcttgag gagataacat gattaaataa
      181 aattttatat cttctttttt agttgcaaac catttttatt aagaatgcta gaaaaagatt
      241 gtttattgat taaagaatta gagataatcg tagttctctt accgtttttt acggtttatc
      301 acatcattat gatgatgaat atcttattaa ttatgctgct atattggggg tgttggctcg
      361 taagacttaa ggcgacagtc cggttatagg ttgtaatgat attgaggagt atcttaaata
      421 tgatagcagt ggcactaaac gagagaatta aagtcatctt aattttagtt ctttttctta
      481 tctaattagt gacgaacttg gacataataa cgtataatgg agatatggaa agaaggtggc
      541 atgatgattt tagattatcc aaaaatttat aaagagaaag aattgattgg tcagggtggt
      601 aatcaagatt ggtttgctga ctcttgggct cgtaaagctg gttgtgcaag tgttagtgct
      661 actgatattt ttatctatta tacgaaaaga gaacgtgaat ttgataaatc aacgtattta
      721 gactacatga atgaaatgtt caagctgatg gaacctggta agcgtggatt tccgtatgtc
      781 tttttatatg caagacgatt gagtaaattg ttaaataatt gtgagtatcg tatttttaga
      841 cgtccaagac tttatgatgc tagtgaaatt gttaaagaga gtattgagca tggtaatcct
      901 ttaggattat tgatcttaac acatcgacgt cgtaagattc gagatgattt atggcactgg
      961 gtaacgattg tcggttacga agaaactaaa aagggtctag atattatctt tttagattgt
     1021 ggtgatgaaa aaaggattcc tgcgcggatt ctttttgaaa aaagtcgttt taatgttgtt
     1081 aagatggttc gtttttttta tcgataaaaa tagtattaaa taacgaaata ttttgatgaa
     1141 ataatgattt aatataatgc aaagaaacac tataaaaatt taataggttc tcttgaaata
     1201 tgttgttagg ggaatcatga tatctatttg gagataacag aagaccgatg aaattattaa
     1261 tcagctatat aattataatt aacttgatga tgatattatt atatggaatt gataagtata
     1321 aagcaattca tcatcattgg cgagtttctg agaagatact attaataggc gctttgtttg
     1381 gtggttcact aggagcgata ttagcgatgt atggatttaa tcataagact aggaaaaata
     1441 tttttaagta tggaatacct ttacttctag gacttcaaat catactgata ataaaaataa
     1501 gcatcggtta atcgatgctt gtttgatata ttgttatttt aatatcaatt gtaatatcta
     1561 atcgttctaa ttcatca
//
LOCUS       DAOMTA010000789         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4149_length_1575_cov_2.182237, whole genome shotgun sequence.
ACCESSION   DAOMTA010000789 DAOMTA010000000
VERSION     DAOMTA010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_1575_cov_2.182237"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..151
                     /locus_tag="PLK68_15670"
     CDS             20..151
                     /locus_tag="PLK68_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM91888.1"
                     /translation="MGLGLAICKAIVEAHKGEIKAFNNDLGGATFELSLPLEEENNE"
     gene            144..827
                     /locus_tag="PLK68_15675"
     CDS             144..827
                     /locus_tag="PLK68_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM91889.1"
                     /translation="MNNTTILIVEDDKTIQNFLKVTLKTQNYNYIIAETGLSGLSLFY
                     ANRPDLVLLDLGLPDIEGIEVLKQIRQNSSIPIIVVSARSSETEKVMALDYGSDDYVT
                     KPFNAAELLARIRAALRHCLKEKVSEPIFELDYLKVDFERRHVWVKDQEIHLTPIEYK
                     MLVLLITNRGKVLTHHFIQENVWGYETTDDYQSLRVFMANIRRKIEIDSSSPHFIITE
                     VGVGYRFVE"
     gene            924..1130
                     /locus_tag="PLK68_15680"
     CDS             924..1130
                     /locus_tag="PLK68_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91890.1"
                     /translation="MLKTAYIYPHNTPSIVNRGKIINYTKYFITDVFCVFKLALLMLL
                     TGALMVGGLFLVVGMPYFVVSLFI"
     gene            complement(1380..1574)
                     /locus_tag="PLK68_15685"
     CDS             complement(1380..1574)
                     /locus_tag="PLK68_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91891.1"
                     /translation="MIILIIKDNTNKVVFISSKQGLSNKIKNFYLDQYINKLIDKNIN
                     LLLYLASIIITEKNYDNYYC"
BASE COUNT          562 a          189 c          262 g          562 t
ORIGIN      
        1 tggtgatcat aaacgtggaa ttgggctagg tctagcaata tgcaaagcaa ttgtcgaagc
       61 gcataagggt gaaatcaagg cctttaataa cgatttaggt ggggcaacat ttgaattaag
      121 cctaccgttg gaggaagaga acaatgaata atacaacaat tctaattgtt gaagatgaca
      181 aaacaattca aaactttcta aaagtaactt taaaaactca aaattataac tatattattg
      241 ccgaaactgg actaagcggt ttatcacttt tctatgccaa tcgaccagat ttagttttac
      301 ttgatttagg tttgccggat attgaaggaa ttgaggtgct taaacaaata cgtcaaaatt
      361 caagtatacc aattatcgtc gtttcagctc gttcatcaga aactgaaaaa gttatggcac
      421 ttgattatgg tagtgatgac tacgtgacta aaccttttaa tgccgcagaa ctattagcac
      481 gaattagagc agcactacgg cattgcttaa aagaaaaagt tagcgaacca atatttgaat
      541 tagattattt gaaagttgat tttgaacgac gacacgtatg ggtcaaagat caagaaattc
      601 atttaacacc aattgaatat aagatgttag tcttattgat tactaatcgt ggaaaagtat
      661 taacccacca ttttattcaa gaaaatgtct ggggatatga aactacagat gattatcaat
      721 cattacgagt atttatggct aatattcgtc gtaaaattga aattgatagt tcctcaccac
      781 atttcattat tactgaagtt ggggtaggat atcggtttgt tgaataatat taatataatc
      841 tttatattta gggctaagat tataaataga aaatttattc ctttccatgc tacgatatca
      901 gtgtagaaag ggagtgatta tatatgttga aaacagcata tatttatcct cataataccc
      961 caagtattgt aaatagagga aagattataa actatacaaa atattttatt actgatgtat
     1021 tttgtgtttt caaattggct ttattaatgc tattgactgg tgcattaatg gttggcggat
     1081 tattccttgt tgtaggaatg ccatactttg ttgtatcatt atttatttaa tcaatttaga
     1141 taaaaagaca tagggtaaaa actctatgtt ttttaataag tatttggtaa ctatcagtgt
     1201 tattaacaaa tttagattta tattttaatg tttttaggaa tagttttaat ttgtttaaaa
     1261 taactaaatt atttttatta aatggaatcg tactagtaat aggcaatcca aaagataatg
     1321 tttgtcttgc aatggtgtta tctgctaaac tatgatcaat tatgaaatta aatagctgtt
     1381 caacaataat aattatcata gtttttttca gtaattatga tagaagctag atataaaaga
     1441 agattaatat ttttatcaat taatttatta atatattgat ctaagtaaaa attttttatt
     1501 ttgttagaca agccttgttt tgaagaaatg aagactactt tatttgtatt atctttgatt
     1561 attaaaatga tgatt
//
LOCUS       DAOMTA010000790         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4157_length_1572_cov_2.036256, whole genome shotgun sequence.
ACCESSION   DAOMTA010000790 DAOMTA010000000
VERSION     DAOMTA010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_1572_cov_2.036256"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(470..1426)
                     /locus_tag="PLK68_15690"
     CDS             complement(470..1426)
                     /locus_tag="PLK68_15690"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HRM91892.1"
                     /translation="MERFQKQPLNLNQQVELLEERGLIISDKERAKRLLGNISYFRLS
                     AYMLPYKIKTDDKIIDKFEDKINWDDIYRLYLFDRKLRLLVFDAIERIEVSVRAQVIS
                     QLSLKYGSHWQDNRDVFKAAHENTLRDGSRVSIDVFSEIQHHINEQLNSNKAEVFIKH
                     YVSKYDEPKNPPSWMCVEIMYFNQLSKICSNLKNRSDINSISQYYALPPDIFCSWLHS
                     INYIRNICAHHARLWNRDMNIVPNKLKFSKKLKWVSDPETTKRGKVYYSLCILNYMLQ
                     TVNHTSNFIPRLKKLMSDYKTVINLNSMGFPDKWEQENLWQD"
BASE COUNT          518 a          249 c          213 g          592 t
ORIGIN      
        1 attacccttg atggttactc ttaactaaca caaatatatc attaagtata cgccctagtt
       61 gtgggttcaa actcaactca ttatttgtgt ctatttaaat atcaaaggtg tctgagctaa
      121 ttttgaataa cgcaaatact tacgtaaatg gaagtacaaa ggtaatgatt taaagcataa
      181 tatatatatt cctacttgtg gagatgtaga aaatcaatgg gaaaatatat ggaaggatta
      241 ataatcaaga aatttaaacg attggaataa atatataata ggtttaaaaa taaaaggcac
      301 caaacaatat gtaaccttat aaaataatta acgcccctca agtatgctgc cttgcggctc
      361 gcctgagagg cttattatct ttgtttttaa taaacgtctt ttaaatgtat tgcaaaggta
      421 tatgtttatt tattgataaa caaatatttt aacctaaaat tcattaatgc taatcttgcc
      481 ataaattttc ctgttcccat ttatcaggga aacccattga atttagattt ataacagttt
      541 tataatcgga catcaatttt ttgagtcttg ggataaaatt agacgtatga ttcactgttt
      601 gtagcatata atttaaaatg cataatgaat aataaacttt gcctcgtttt gttgtctctg
      661 gatctgaaac ccattttaac tttttggaaa attttaattt attaggtaca atattcatat
      721 cacgattcca caatcgagca tggtgggcac aaatgtttct tatataattg attgaatgaa
      781 gccaagaaca aaatatatca ggtggcaatg cataatattg tgatatgcta ttaatgtccg
      841 aacgattctt aagattagaa caaatttttg acaactgatt gaaatacatt atttcaacac
      901 acatccatga tggcggattc tttggttcat catattttga tacataatgc tttataaaaa
      961 cttctgcttt attgctattt agttgttcat ttatatgatg ctggatttcg gaaaagacgt
     1021 ctattgaaac tcggcttcca tctcttaacg tattttcatg tgccgcttta aatacgtctc
     1081 tattatcctg ccaatgtgaa ccatatttta agctcaactg agatataacc tgcgctctta
     1141 ctgacacctc aattctttcg atagcatcaa atactaaaag tcttaatttc ctatcgaaaa
     1201 ggtacaatct atatatgtca tcccaattaa ttttatcctc aaatttatct attattttat
     1261 cgtctgtctt tattttatat ggtagcatat aagcacttaa tctaaaatag cttatattac
     1321 ccagtaaccg ctttgcgcgc tctttatcag aaattattaa tcctctctct tctagaagtt
     1381 ctacttgttg atttaaattt aatggttgtt tttggaatct ttccattctt ttcgttttat
     1441 attgcaaata tagttaatta tactatgtaa gcatccccct tttattatat caaattaaac
     1501 ttttacttaa atataattta tgacaaagct atacacagat gtatacattt tcttttattc
     1561 ggtcttgtaa tt
//
LOCUS       DAOMTA010000791         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4161_length_1571_cov_2.192612, whole genome shotgun sequence.
ACCESSION   DAOMTA010000791 DAOMTA010000000
VERSION     DAOMTA010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4161_length_1571_cov_2.192612"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLK68_15695"
     CDS             complement(<1..632)
                     /locus_tag="PLK68_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HRM91893.1"
                     /translation="MCKKLSIILLCLLMITGCSKDKPILYSNLGNQASQNKLTNILNE
                     ADLPKENIKQFFSYVNTFNQHAIPLIGDFETLEREQPDYQYFKYNSPVEISDQNDSNS
                     LIPSFILIKNLIYTNNTGHADDSYIMFNLNLIDTIDQYSMSQEDRLKFITTFNSISVT
                     GIKNNEISHINQIEKTFSDRDFSVKQNQKASLITLWLHSSADNRRFVSHCG"
     gene            749..1267
                     /locus_tag="PLK68_15700"
     CDS             749..1267
                     /locus_tag="PLK68_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRM91894.1"
                     /translation="MNETINNLITRRSVRSFQKEQISEEQLNEILTAGTYAASGMGRQ
                     SAIMVVIQNPEIIKRLSKLNAAIMDSENDPFYGAPTVVVVLGDSTIGTYLEDGALVMG
                     NLLNAANAVGVDSCWIHRAKEVFMSTEGKELLKQWGIDEKYVGIGNCILGYGKEPKPE
                     AKPRKDNYIYRV"
     gene            1308..1433
                     /locus_tag="PLK68_15705"
     CDS             1308..1433
                     /locus_tag="PLK68_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91895.1"
                     /translation="MKYVLTHATVFDGYHDYVLKHDLNIYLDESSIKAFSVIRLV"
BASE COUNT          536 a          201 c          303 g          531 t
ORIGIN      
        1 ccacagtggc taacaaagcg acggttatct gcggaagagt gaagccacaa ggtaatcaat
       61 gatgcttttt ggttttgttt tacgctaaag tcacgatcac taaatgtttt ttcaatttga
      121 ttgatatgag aaatttcatt attttttatg ccggtaacag aaatgctgtt aaaagtagta
      181 atgaatttta aacgatcttc ttggctcatt gaatactgat caatagtatc aattaaattt
      241 aaattaaaca taatatatga gtcatctgcg tgaccggtat tattagtata aattaggttt
      301 tttattaaga taaatgaagg aattagtgaa ttagaatcgt tttgatcact tatttcaact
      361 gggctattat atttaaaata ttggtaatct ggttgttctc tttctaaagt ttcaaaatca
      421 ccaattaagg gtatagcgtg ctgattaaag gtattaacat aggaaaagaa ttgtttaata
      481 ttctctttgg ggagatcagc ttcatttaaa atattagtaa gtttattttg actggcttga
      541 ttaccaagat tgctatataa aattggcttg tctttagagc atcctgttat cataagcagg
      601 catagtaaaa ttattgatag ttttttacac ataaacagcc ccccttttat ctaaattata
      661 tagagtattt ttcttaaaaa caattatcta tatcttaata ttcattaagt ctgtgatata
      721 atatccttat aaaaaggggg atagtataat gaatgaaaca atcaataatt taattacacg
      781 tcgaagtgtt cgaagcttcc aaaaagagca aattagcgaa gaacaattga atgaaatttt
      841 gacggctgga acatatgcag cctcaggaat gggacgtcaa tctgcaatta tggtagttat
      901 tcaaaatcct gagataatta agcggctatc aaaattgaat gcggcaataa tggatagtga
      961 aaacgatcct ttttatggag caccaacagt ggtagtcgtt ttaggggatt caacgattgg
     1021 aacatattta gaagatggtg cgttagtaat ggggaatctt ttaaatgcag ccaatgcagt
     1081 tggcgtagat tcttgctgga ttcatcgtgc caaagaagtt tttatgagta ctgagggaaa
     1141 agaattgtta aagcagtggg gcattgatga aaagtatgta ggaatcggta attgtatttt
     1201 aggttatggt aaagaaccga aaccagaagc taaaccgcga aaagataatt atatttatcg
     1261 tgtctaggaa tcgcaaagat tccttttttt aaaagagagt gaatgcaatg aagtatgtat
     1321 taactcatgc aactgttttt gatggttatc atgattatgt gttgaaacat gatcttaata
     1381 tctatcttga tgaatcatca ataaaagctt ttagtgtcat caggctagtt taatttgcac
     1441 tttattacca gcggtgttac tcgctaattt ttcgctagaa cagatgtttg tggtaagatg
     1501 atagatcatc caaaaataaa aaacgtggga gagcaattag atactttgct ataaagatat
     1561 aagagttaat c
//
LOCUS       DAOMTA010000792         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4170_length_1569_cov_2.632100, whole genome shotgun sequence.
ACCESSION   DAOMTA010000792 DAOMTA010000000
VERSION     DAOMTA010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_1569_cov_2.632100"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..>1569
                     /locus_tag="PLK68_15710"
     CDS             66..>1569
                     /locus_tag="PLK68_15710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91896.1"
                     /translation="MKRRKKEFGIYMILGMSKKQISKILLVETITIGLISLVIGLVLG
                     VALSQVMSIVVANMFEADMTKFTFVFSLSAWIKTMIYFGIMYLLVMVFNTIQVNRQQL
                     IKLLTANRQNEEVKLKNTYLCIGIFIGAVVLLSYAYYNVTAGAGNLTNSSSVLFQMIY
                     GAVATFLIYWSISGLILKFVMASKNYYFKNLNSFTVKQISSKINTTVLSTTVICLMLF
                     LTICIFSSAFALNNSATEEINELAPVDIQMQKDVSVDNLSITKYLSQKQIGLDDLKNI
                     YTFMTYRTSQLTYRDTFGSAFKDVSESYLNNCEDIIKLSDYNKLAKIYNLPTYDLKDE
                     YVVMGNYENLMFSRNQFLKEKPAIELNGKIYQSKFDKCQNGFLSMQSSHMCMGVYIVP
                     DEAVSNFIPADSYLVGNYAANDQDTRNEVDEKMHSYSSDVLLINTRIQISSSSVSMGA
                     MVIFIGLYIGIVFLISCAAILALKELSQSIDNKGKYQILRNVGVDEKMINR"
BASE COUNT          550 a          166 c          284 g          569 t
ORIGIN      
        1 aaaaatcatc gctgttgata agaacagact tataggtggt atggctaata ttgcaggcat
       61 cgttaatgaa gcgaagaaaa aaagagtttg ggatatatat gatattagga atgtcaaaaa
      121 aacagatttc taaaatacta ttagttgaaa ctataacaat tggtttgatt tcattagtaa
      181 ttggtttagt cttaggagtc gctctatctc aagtaatgtc gattgttgta gctaatatgt
      241 ttgaagcaga catgactaaa tttacatttg ttttctcttt aagtgcgtgg ataaaaacaa
      301 tgatttattt tgggattatg tatcttctgg ttatggtttt taatacgatt caagtgaatc
      361 gccaacaatt aattaaatta ttgacagcaa atagacaaaa tgaagaagtt aaattaaaga
      421 atacctatct ttgtataggg atctttattg gtgctgttgt attattaagt tatgcttatt
      481 ataacgttac cgcaggagct ggtaatttaa cgaacagcag ctcagtgctt ttccaaatga
      541 tttatggtgc tgtcgcaacg tttttaatct attggtctat ttcaggtttg attttaaaat
      601 ttgttatggc atctaaaaat tattatttca aaaatttaaa tagttttacg gttaaacaaa
      661 ttagtagtaa gattaataca acagtacttt caacaacggt aatttgttta atgctttttt
      721 taacgatctg tattttttca agtgcttttg cgttaaataa ttctgcaaca gaagagataa
      781 atgagttagc tccagttgat attcaaatgc aaaaagatgt aagtgttgat aatttatcaa
      841 ttacaaaata tttgtctcaa aagcaaatag gattagatga tttaaaaaac atttatacat
      901 ttatgactta tcgtacttca caattgacat atagagatac ttttggcagt gcctttaaag
      961 atgtaagtga aagttatttg aataattgtg aagatattat taaacttagt gattataata
     1021 aattggctaa aatatataat ttaccaactt atgatttaaa agacgaatat gttgtaatgg
     1081 ggaattatga aaatttaatg tttagtcgaa atcaatttct taaagaaaaa ccagctattg
     1141 agttaaacgg aaagatatat cagagtaaat ttgataaatg tcaaaatggt tttttatcaa
     1201 tgcagtctag tcatatgtgt atgggagtgt atattgttcc agatgaagcg gttagtaatt
     1261 ttattcctgc tgatagttat ttagtgggta attatgctgc aaatgatcag gatacaagaa
     1321 acgaagtaga tgaaaaaatg cacagttata gctctgatgt cttattgatt aatacaagaa
     1381 ttcaaatttc aagtagtagt gtgtctatgg gagctatggt tatctttatt ggtttatata
     1441 taggaattgt atttttgatt tcttgtgctg ctatcttggc actcaaagag ttgtcacaaa
     1501 gcattgataa taaaggaaaa tatcagattt taaggaacgt tggtgttgat gaaaaaatga
     1561 ttaatcgtt
//
LOCUS       DAOMTA010000793         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4180_length_1566_cov_2.135010, whole genome shotgun sequence.
ACCESSION   DAOMTA010000793 DAOMTA010000000
VERSION     DAOMTA010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4180_length_1566_cov_2.135010"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..588
                     /gene="nusB"
                     /locus_tag="PLK68_15715"
     CDS             184..588
                     /gene="nusB"
                     /locus_tag="PLK68_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRM91897.1"
                     /translation="MKKYRKKIIREKAVIATYQKLLVDTNEDEIREYLNSDKDLSSNQ
                     DDFDYCFMFIISIASNIERYKAEVAKYLKPGWTLDRLSKMELAILLVGCYELLETEQS
                     KEVIINEAVELSKKYCDSDAYKFINGLLNRIK"
     gene            588..>1566
                     /gene="xseA"
                     /locus_tag="PLK68_15720"
     CDS             588..>1566
                     /gene="xseA"
                     /locus_tag="PLK68_15720"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRM91898.1"
                     /translation="MEKRYLTVSALNRYLKAKIDSDSQLQRILIKGEVSNFKHHSSGH
                     FYFTLKDEHSRINAVMFSSKASKVPFDLTNGMKVLVQASVSVYDVAGTYQLYVDTIEQ
                     DGLGNLFLKYEQLKKQLANEGLFNPENKLVIPKFPSKIAVLSAYPSAALADIIRTIHL
                     RFPVVRVIVFPIPVQGKDAYLEIIRTLRYVDTLGFSEIIIARGGGSLEDLWNFNEEGL
                     ARAIYQCKTPIISGVGHEVDFTICDFVADYRAATPTAAAIKATPDLFELQQAVDNIKY
                     TLNNLMKQKIILNKENLNRLKSFYLFKNPQKMFEDKTAKIDYLYDQLNNV"
BASE COUNT          579 a          178 c          277 g          532 t
ORIGIN      
        1 aatagctcaa agctgttttg aaatgacagg aatcaaaaat gttaaagtaa atattgaagt
       61 taaaggaatt aatttttaag gttatcttcg gataatcttt tttgttttat tatgatgcat
      121 ttcgtttatt ttaaggtgca aatatggtaa aatcatgata gatattttgg aaggacggat
      181 tagatgaaaa aatatagaaa aaaaataatt agagaaaaag cagtaattgc aacttatcaa
      241 aaactgctag tagatacaaa tgaagatgag attcgtgaat atttaaacag tgataaggat
      301 ttatctagca atcaagatga ttttgattat tgttttatgt ttattataag tattgctagt
      361 aacattgaac gttataaagc tgaagttgct aaatatttaa aacccggatg gacgttagat
      421 agattatcaa aaatggaact tgcaatttta ttagttggat gttatgaact tttagaaact
      481 gaacaaagta aagaagtaat cattaatgaa gcggttgaat tatcaaaaaa atattgtgat
      541 agtgatgctt ataaattcat taatggatta cttaatagga ttaaatagtg gaaaaacgat
      601 atctgacggt tagtgcactt aatcgttatt taaaagctaa aattgattct gatagtcaat
      661 tacagcgaat cttaatcaaa ggtgaggttt ctaattttaa acatcatagt tcaggacatt
      721 tttattttac gctaaaagat gagcattcaa gaataaatgc agtaatgttc tcttcaaaag
      781 ctagtaaagt tccttttgat ttgaccaatg ggatgaaagt acttgttcaa gctagtgtgt
      841 cagtatatga tgtagcaggc acctatcaat tgtatgttga tacaattgaa caagatggat
      901 taggaaattt atttttaaaa tatgaacaat taaaaaagca gttagctaac gaaggtttat
      961 ttaaccctga aaataaatta gtaattccta aatttcctag taagattgca gttttgtcag
     1021 cctatcctag tgctgctctt gctgatatta ttagaaccat tcacttgcgt tttcctgtcg
     1081 ttcgtgtaat cgtatttcca attccagtcc agggtaagga tgcatatttg gaaataatta
     1141 gaacacttag atatgttgat acactaggat ttagtgaaat tattattgca agaggcggag
     1201 gttcattaga agacttatgg aacttcaacg aggaaggatt agctcgagca atttatcaat
     1261 gtaagacacc tattattagc ggtgttggtc atgaagttga ttttactatt tgtgattttg
     1321 ttgcagatta tcgtgctgca acaccaacgg ctgcggcaat taaagcaaca ccggacttat
     1381 ttgaattaca gcaggctgta gacaatataa aatatacttt aaataattta atgaaacaaa
     1441 aaattatatt aaataaagaa aatttaaatc gattaaagtc attctattta tttaaaaatc
     1501 ctcaaaaaat gtttgaagat aagacggcta aaattgatta tttatatgat caattaaata
     1561 atgttt
//
LOCUS       DAOMTA010000794         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4190_length_1564_cov_2.194168, whole genome shotgun sequence.
ACCESSION   DAOMTA010000794 DAOMTA010000000
VERSION     DAOMTA010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4190_length_1564_cov_2.194168"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLK68_15725"
     CDS             complement(<1..466)
                     /locus_tag="PLK68_15725"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRM91899.1"
                     /translation="MPIKIPNDLPASKILKEENIFVMDENRALAQQIRPLQLLILNIM
                     PTKVVTETQLLRMLSNTPLQIEVDWIHMASHESKNTPQEHLLAFYKTFEEIKDNKYDG
                     LIITGAPVEKLRFEDVDYWLEMEKILEWSKTHVFSSFFICWASQAALHYFYGI"
     gene            complement(556..>1564)
                     /locus_tag="PLK68_15730"
     CDS             complement(556..>1564)
                     /locus_tag="PLK68_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM91900.1"
                     /translation="VESVSYTIANYLGLNTELTKAIAVGHDLGHAPFGHGGERIINEL
                     AKIHGLDSFWHERNSLHLIDEIETLEDNEHHRHNLNLTYAIRDGIISHCGEMNQMSIK
                     KRDEYIDLQNYTSPGQYNPYTWEGCVVKMADKIAYLARDIEDALRLKVLKENKVEVLK
                     ASLNNITSEYHFTAINNGTVVNYFIQDVCSHSNPSDGISLSKEAFEIMKTIMKFNYQN
                     IYLIDRVQIHTNYVKLILNSIFDFFIKYDLMARDSNTNIIDEISKDKDQFPHAINGFI
                     HWLEKYSVMNGAKRDSLYQNKVIYDFINDDKAIIKSILDYLSGMSDAYIIQIFNELIS
                     F"
BASE COUNT          538 a          265 c          191 g          570 t
ORIGIN      
        1 caataccata aaaataatgc aatgctgctt gagaagccca acaaataaaa aatgagctaa
       61 atacatgagt ttttgaccat tctaaaatct tttccatctc taaccagtaa tcaacatctt
      121 caaacctaag tttttccacc ggtgctccag taataatcaa accatcatat ttattatctt
      181 ttatttcctc aaatgtctta taaaaagcta ataagtgttc ttgaggtgtg tttttagatt
      241 catgactggc catatgaatc cagtctacct cgatttgaag tggtgtattc gacagcatcc
      301 ttaaaagctg tgtttcagtt actactttag tcggcataat atttaaaatt aataattgca
      361 atggcctaat ttgttgagct aaagcacgat tttcatccat cacaaagata ttttcttctt
      421 ttaatatttt acttgctggt aaatcatttg gtattttaat aggcatattt aaacctccct
      481 aataatgatt agtttaccac atctattcta ataatttaag taaaaataat aaatccattg
      541 aatttcttca atggattaaa atgaaataag ctcattaaat atttgaataa tataggcatc
      601 tgacattcct gataaataat ctaaaatact tttaataatt gctttatcat cattaataaa
      661 atcataaata actttatttt gataaagcga atcacgtttt gctccattca tcacactata
      721 tttttcaagc caatggataa atccattgat tgcatgggga aattgatctt tatccttaga
      781 aatttcatca ataatattag tattactatc tcgagccatt aaatcgtatt taataaagaa
      841 atcaaaaata gaatttaaaa tcaatttaac ataattagta tgaatttgta ctctatcaat
      901 taaatatata ttttgataat taaacttcat aattgttttc attatttcaa aggcttcttt
      961 tgataatgaa attccatcgc ttggattact atgactgcat acatcttgaa taaaataatt
     1021 aacgacagtt ccattattga ttgctgtaaa atgatattca ctagtaatat tattgagcga
     1081 agctttcaag acttcaactt tattctcttt tagtaccttg agacgtaaag cgtcttcaat
     1141 atcacgtgct aaataggcaa tcttatctgc catttttaca acacatcctt cccaagtata
     1201 aggattgtat tgtcctgggc tagtataatt ttgtaaatca atatactcat ctcgcttttt
     1261 aatactcatt tgattcattt ctccacaatg agaaataatt ccatcacgaa tagcatatgt
     1321 caaatttaaa ttatggcgat gatgttcatt atcttctaat gtttctattt catcaatgag
     1381 atgaagacta ttgcgctcat gccaaaaaga atcgagccca tgtattttag caagctcgtt
     1441 aataatacgt tcaccaccat gaccaaatgg ggcatgtcct aaatcatggc ctactgctat
     1501 tgctttagtt aattcagtat tcaaacctaa gtaatttgca attgtataac ttacagattc
     1561 aact
//
LOCUS       DAOMTA010000795         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4195_length_1563_cov_2.229443, whole genome shotgun sequence.
ACCESSION   DAOMTA010000795 DAOMTA010000000
VERSION     DAOMTA010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4195_length_1563_cov_2.229443"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLK68_15735"
     CDS             complement(<1..344)
                     /locus_tag="PLK68_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein GerQ"
                     /protein_id="HRM91901.1"
                     /translation="MNYFEDLNPYFVRQDPDAEVNPDGTLIPPKNPNPAAVPQIYMGN
                     IIRLNIGKLGTFYFTYSDSEKWRDEVFVGIVEDAGRDYFIIKDVNSEDRFLLPLVYFL
                     WARFKGAISFTVP"
     gene            complement(358..780)
                     /locus_tag="PLK68_15740"
     CDS             complement(358..780)
                     /locus_tag="PLK68_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRM91902.1"
                     /translation="MSSRIAYNSKEVELLARLIKAEALGEGQEGMLLVGNVVVNRVAA
                     VCDVFKNVVTITEAIYQKNAFAGVGTDLFNGPVNTKEKELALKTIKGYRNAPATYALW
                     FKNPGDNVTCPKTFYGEFSGRFKNHCFYNPGPNLVCEI"
     gene            1066..1425
                     /locus_tag="PLK68_15745"
     CDS             1066..1425
                     /locus_tag="PLK68_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91903.1"
                     /translation="MNKREIEKQALNSLNTYFKDNPDKVLNDFERKPLDKEDITKIVN
                     EEIQAQEKKVESLEVLRKNIAIEAEESLEKVLGKRITSKKSPNKLTVDEMAVKLQEEK
                     THQIETANKLKTAKLRK"
BASE COUNT          602 a          266 c          212 g          483 t
ORIGIN      
        1 ggtaccgtaa acgagattgc gcccttaaat cgagcccata aaaaatatac taacggtaat
       61 aaaaaccgat cttcactatt tacatcttta ataataaaat agtctcgacc tgcatcctca
      121 actataccaa caaaaacttc atctcgccat ttctcactat cactataagt gaaataaaaa
      181 gtcccaagct taccgatatt caaacgaatg atatttccca tatatatttg tggtacagct
      241 gccggattag ggtttttagg cggtatcagt gtaccgtcag gattcacttc agcatctgga
      301 tcctgtctaa caaaataagg atttaaatct tcaaaataat tcataaatgc atctccttta
      361 gatttcacat actaaatttg gtccgggatt ataaaaacaa tgattcttaa atcgacccga
      421 aaattctcca taaaatgttt ttgggcaagt aacattatcc ccagggtttt tgaaccataa
      481 cgcatatgtt gctggagcat tccgataacc tttaattgtt ttaagcgcta gttctttttc
      541 tttggtatta acaggtccat taaataaatc tgttccaact ccagcaaaag catttttctg
      601 ataaattgct tccgtaatag taacaacatt tttaaataca tcacaaactg cagcaactct
      661 attaacgaca acattcccaa ctaacagcat tccttcttga ccttcgccta atgcttcagc
      721 cttgatcaac cgtgctagta attctacttc cttactgttg taagcaatcc ttgagctcat
      781 cttctgatat cacctcttta agactatatg ccgataaagc aacaatataa ctaatttata
      841 cagtaattta agcttttttc gacttattca aaaaatatta ttagatataa aattaatctt
      901 tcgaataata actttattcc cgatattttg ttaacaacat taacagaact atgataaaaa
      961 aacttctaat tttgttaata gtatttaaat caactatttt ttactttcca aaattatcag
     1021 taacgattat gatacgagtg tagttaagga aagaaggtaa ttaaaatgaa taagagagaa
     1081 attgaaaaac aagcactcaa ttcattaaac acttacttta aagataatcc cgataaagta
     1141 ttgaatgatt ttgagcgaaa gcctttggat aaagaagata taactaaaat tgtcaacgaa
     1201 gaaatccaag ctcaagaaaa aaaagttgaa tcacttgaag tgctgagaaa aaatattgct
     1261 attgaggcag aagaatcgtt agaaaaggtt ttaggtaaaa ggattaccag caaaaaatct
     1321 ccaaataaat taactgttga tgagatggca gttaaactac aagaagaaaa aactcatcaa
     1381 attgaaactg caaataaatt aaaaacagct aaattaagga aataacattt gttaatgcca
     1441 ttaacaaata atctaaattg aaagtagaaa ttttgttaac agcatttata aatgattatt
     1501 atatatagaa ttattttgca cagaagcata tattacaaat gaaaacaaaa gaaagaggga
     1561 aat
//
LOCUS       DAOMTA010000796         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4205_length_1562_cov_1.437293, whole genome shotgun sequence.
ACCESSION   DAOMTA010000796 DAOMTA010000000
VERSION     DAOMTA010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_1562_cov_1.437293"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..>1562
                     /locus_tag="PLK68_15750"
     CDS             173..>1562
                     /locus_tag="PLK68_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM91904.1"
                     /translation="MSKKKKLTKTLAALSAAGCIIGSATPIALNAVELSKYSVVEAKY
                     QAAKTEGSGENAQFSDYDIDIMVVTNPDYKGEAAPTLTYSKAFDEKAKEAGKTALLSE
                     NIDGKEYAFKDMNLNGEIDDYERWYLDAETRAQDLADALVAEGEDGIAKIAGLMLFSS
                     HEGNSAAGLTDSQKDYLTNSYVRNITDAAGNDVEDSVRWVNAMQEYVEISDFNVPVDF
                     SSDPRSTAGSGDLYSDNVSSGVTDISKWPSNIGLAATFDTEHMYNFAKASSAEYRALG
                     IVTALGPQIDLATEPRWLRVGGTFGENTKLSTDMAQAYVDGSQSTYVNGEDQGWGKDS
                     INCMIKHFPGDGAGEGGRESHTRAGKYAVYPGGNFEEHLTPFAQGGLKLKGKTGAATA
                     VMTSYSIGINADGSALGGERVGSAYSKSKMSILRDDLGFDGVVCTDWGVTSTPPEQLA
                     LGGLGMAWGMENA"
BASE COUNT          523 a          213 c          359 g          467 t
ORIGIN      
        1 ataaatagat ttattttttt tataaatttg gtctaagaaa aaatactttt tttcacgaaa
       61 acaaaaaagg ttaaacttta tttgtgtttt gtcgatgatt aagtaaacgc tttcatcaac
      121 ttctcgttta tactagaagc atagggaatt taaacgagga ggaaaatcga agatgagtaa
      181 gaagaaaaaa cttacaaaaa cattggctgc tttatcagct gccggctgta ttatcggaag
      241 tgcaacaccg attgcattaa atgcagtaga attatcaaaa tattctgttg tggaagcaaa
      301 atatcaggct gcgaaaacag aaggaagcgg agaaaatgct caatttagtg actacgatat
      361 tgatattatg gtagtaacta atcctgatta taaaggtgaa gcagctccaa ctttgactta
      421 ttcaaaagct tttgatgaaa aagctaaaga agctggtaaa acggcgttgt taagcgaaaa
      481 tatcgatggt aaggaatatg ctttcaaaga tatgaactta aatggagaaa tcgatgatta
      541 tgaaagatgg tatcttgatg ctgaaacaag agcacaagat cttgctgatg cattggttgc
      601 tgaaggtgaa gatgggatcg caaagattgc tggattaatg ttatttagta gccatgaagg
      661 aaatagtgct gcaggattaa cagattcaca aaaagattat ttaacaaata gttatgttag
      721 aaatattact gatgcagcag gaaatgatgt ggaagattca gtacgttggg tcaatgcgat
      781 gcaagagtat gtagaaatat ctgatttcaa tgttcctgtt gattttagtt ctgatcctcg
      841 ttcaacagca gggtcaggag atttatattc tgataatgtt tctagtgggg ttacagatat
      901 ttctaaatgg ccttctaata ttggattggc agcaactttt gatactgaac atatgtataa
      961 ttttgcaaag gcatcttcag ctgaatatcg tgcattagga attgtaacag ctttaggacc
     1021 tcaaatagat ttagcgacag aaccacgctg gttaagagtt ggtggaactt ttggtgaaaa
     1081 tactaaacta tctacagata tggctcaagc ttatgtagac ggttcacaat caacatatgt
     1141 taatggagaa gatcaaggat ggggaaaaga ttcaattaac tgtatgatca aacacttccc
     1201 tggtgatgga gccggtgaag gtggtcgtga atcacatacc cgtgcaggta aatatgcagt
     1261 ttatccaggt ggtaattttg aagaacattt aactcctttt gctcaaggtg gcttaaaatt
     1321 gaaagggaaa actggagcgg caactgcagt gatgacatct tattcaattg gaatcaatgc
     1381 tgacggtagt gctttaggtg gcgaaagagt tggttcagct tatagtaagt ctaaaatgag
     1441 tattttaaga gatgatttag gctttgacgg tgtagtttgt actgactggg gtgttactag
     1501 cacgccccct gaacaattag cactaggtgg tctaggaatg gcttggggaa tggaaaatgc
     1561 ta
//
LOCUS       DAOMTA010000797         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4208_length_1561_cov_2.432935, whole genome shotgun sequence.
ACCESSION   DAOMTA010000797 DAOMTA010000000
VERSION     DAOMTA010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_1561_cov_2.432935"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /gene="ricT"
                     /locus_tag="PLK68_15755"
     CDS             complement(<1..688)
                     /gene="ricT"
                     /locus_tag="PLK68_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRM91905.1"
                     /translation="MEKIKLASVKFKSAGKVYYFSTDIELDKGDKVVVETARGVELGE
                     ISQSLKSISEFNLDTELKKIARKATQRDIEAYKKNIIDAQEALVTCRDIISRYDVDMQ
                     LTNCEFTLDKAKVIFMYTSDVRVDFRELLKELATVFRCRIELRQIGPRDKAKVIGGMG
                     TCGLPLCCTSLLGEFNGVSINMAKNQMLAINIEKISGACGRLMCCLKYEDEVYTIEKQ
                     RFPKIGSRVKY"
     gene            complement(701..>1561)
                     /locus_tag="PLK68_15760"
     CDS             complement(701..>1561)
                     /locus_tag="PLK68_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91906.1"
                     /translation="AFLLIGNNTNIPLTYLAMSLICDETLACENCNDCRKVKENKYSD
                     IIRFNGKDNSIKKGNIELIQDTFKKSSLEGKAKIYIIENIEYATKEAMNTLLKMLEEP
                     TEGIYAIFTANNASRVLPTILSRCQVIDIKPDSKEVIIGALCQDGITKESAHILAYLA
                     PSIEEAKTLYDERFEYMQLQVINFIEDLFLKRANLIINTQTNLLKKYKERDDIKLFLN
                     MLVLAMKDMFHVKHTDDIVYCEHQEFLRNIKIDESQLIKQIEIILETIYTIESNANVP
                     MLMDSMMYRL"
BASE COUNT          512 a          295 c          163 g          591 t
ORIGIN      
        1 catattttac tcgactgcca attttaggaa aacgttgttt ctcaattgta taaacttcat
       61 cttcatactt aagacagcac atcaaacgac cacaagctcc agatattttt tcaatattaa
      121 tagctagcat ttgatttttt gccatattaa ttgatactcc attaaattca ccaagtaatg
      181 atgtgcagca aagtggtaat ccacaagttc ccatcccacc tattactttt gctttatctc
      241 ttggtcctat ttgtcgtaat tcaatacgac atctaaatac cgtagctaat tcttttaata
      301 attctctaaa atctacccgt acatctgatg tatacataaa aatcactttt gccttatcta
      361 gtgtaaattc gcaattagtc aattgcatgt ctacatcata acgtgaaata atatcacggc
      421 aagtaactag agcttcttgg gcatcaatta tatttttctt ataggcttca atatcacgtt
      481 gtgtcgcttt tcttgcaatt ttttttaatt ctgtatctaa attaaattca cttattgatt
      541 ttaatgactg tgaaatctca ccaagttcta ctccacgtgc tgtttcaact actactttat
      601 cacctttatc taattcgata tctgttgaaa aataataaac tttaccagca cttttaaatt
      661 taacacttgc caatttaatt ttttccataa tatctccctt ctatagtcta tacatcatac
      721 tatccatcag catcggtaca ttagcattgc tttcgatagt ataaatagtt tctaaaataa
      781 tttctatttg tttaatcaat tgactctcat caatttttat atttctcaaa aattcttgat
      841 gttcacaata tacaatatca tcagtatgtt tcacgtgaaa catatctttc atcgctaata
      901 ctaacatatt taaaaacaat tttatatcat cacgctcttt atattttttt aataaattag
      961 tctgagtatt aataatcaaa ttagctctct ttaaaaataa atcctcaata aaatttatta
     1021 cttgcaattg catatattca aagcgttcat cgtataaagt cttagcttct tctatactag
     1081 gggccaaata agctaaaata tgagcagatt ccttagtaat gccatcttgg cacaaagcac
     1141 caattataac ctctttgcta tcaggcttaa tatcaattac ctgacatcta gataaaatag
     1201 ttggtaatac ccgactagca ttattagcag taaaaattgc ataaatcccc tcggttggct
     1261 cttcaagcat cttaagtaac gtattcatcg cttcttttgt agcatattca atattctcaa
     1321 ttatatatat cttagctttt ccttctaaag aagacttttt aaaggtatct tgaatcagtt
     1381 caatattacc cttttttata gaattatctt taccattaaa tcgaataata tcactatatt
     1441 tattttcttt aactttacga caatcattac aattttcgca agctaatgtt tcatcacaaa
     1501 tcaatgacat tgcaagataa gtcaaaggaa tgttagtatt atttccaatt aataaaaaag
     1561 c
//
LOCUS       DAOMTA010000798         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4218_length_1559_cov_2.146941, whole genome shotgun sequence.
ACCESSION   DAOMTA010000798 DAOMTA010000000
VERSION     DAOMTA010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_1559_cov_2.146941"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLK68_15765"
     CDS             complement(<1..509)
                     /locus_tag="PLK68_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM91907.1"
                     /translation="MAKSNTIELIEQAHRGDDRAKELVVSQNLGLVWSIVHRFKNNYY
                     DKEDLFQIGCIGLMKAVNNFDTNYGVQFSTYAVPIIMGEIKRYFRDDGTIKVSRSLKE
                     LNLKINKARELLTNQTGQDPTVEQIAAYLDVDVQDVVEAIDSSYYPTSLSEPIYEKDG
                     SSISMEERI"
     gene            complement(502..936)
                     /gene="spoIIAB"
                     /locus_tag="PLK68_15770"
     CDS             complement(502..936)
                     /gene="spoIIAB"
                     /locus_tag="PLK68_15770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HRM91908.1"
                     /translation="MNQMELSFNARIENEPFARTSVASFITSLNPTIDELVEIKTIIS
                     EGVSNAIIHGYQNNPECKVIIKVTIEDNRQIKIIIQDYGKGIEDIDEAKIPMYTSLKE
                     LEHAGMGLTIIEALCDNLEIHSTPDLGTKLTIKKQLKDSNLG"
     gene            complement(936..1259)
                     /locus_tag="PLK68_15775"
     CDS             complement(936..1259)
                     /locus_tag="PLK68_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRM91909.1"
                     /translation="MENKIEYKILDDKVIVYFYGELSCSNVVKYRSLLNSILDKGNGP
                     VYFDFLHTNFIDSSGIGLVLGRYNQLKLDDRQLYLANLSKTSYKVFELSGMFDLMEYV
                     EEVKG"
BASE COUNT          514 a          277 c          208 g          560 t
ORIGIN      
        1 tcaattcgtt cctccattga aatagaagaa ccgtcctttt cataaattgg ttcacttaat
       61 gaggttggat aataagatga gtcaattgcc tcaacaacat cttgaacatc aacatctaaa
      121 taagctgcaa tttgttcaac tgttggatct tgaccggttt gattagttag taattcacga
      181 gctttattta tttttaaatt aagttctttt aaagagcgac tcactttaat tgtcccatca
      241 tctctaaaat aacgtttgat ttcacccatg atgattggta ctgcatatgt agagaattgt
      301 accccatagt tagtatcaaa gttattaaca gctttcatca aaccaataca tccaatttga
      361 aaaagatctt ctttatcata ataattattt ttgaaacgat ggactattga ccaaactagt
      421 ccaagatttt gactgacaac taattcttta gcacgatcat ccccgcgatg tgcttgttca
      481 atcagttcaa tagtattact tttagccaag gttagagtct ttcaattgtt tcttaatagt
      541 tagtttagtt cctaaatccg gtgttgaatg tatttctaaa ttatcgcaaa gggcttctat
      601 aatggttaac cccatccccg catgttctaa ttcttttaat gaggtataca taggtatttt
      661 tgcttcatca atatcttcaa ttccttttcc ataatcttgg ataatgattt taatttgacg
      721 attgtcttct attgttactt taataattac cttacattca ggattatttt gataaccatg
      781 gataatggca ttactaaccc cttcactaat gattgttttt atttcaacta attcatcgat
      841 tgtaggattt aatgaggtaa taaatgatgc aacgctagtt cgcgcaaagg gctcattttc
      901 aattctagcg ttaaaactta actccatctg gttcattatc cttttacctc ctctacatat
      961 tccatcaaat caaacattcc tgataattca aaaactttat aagatgtttt tgataaattt
     1021 gctaaatata attggcgatc atctaatttc aattgattat aacgtcctaa aactaatcca
     1081 atgcctgaac tatcaataaa attagtatgt aaaaaatcaa aatagacagg tccattgccc
     1141 ttatctaaaa tactatttag taagctgcga tatttaacaa cattactgca gctaagttcg
     1201 ccataaaaat agacgataac tttatcatct aatatcttgt attcaatctt attttccatt
     1261 atttcatctc ctcgtttgta tcttacatct tttaaaataa tgatgaaatg gtttcgacaa
     1321 aactagaaga aagcatacaa aaaaagtagt cataaacaaa tttcataact actagatgta
     1381 tttttatcaa acttggacat attaattagg gaggcgataa aattgatgaa aaaattacta
     1441 ataaccctac tggtagtatg cttactacca atctctagag ttcaagcatt ggacaatgat
     1501 gttactccta atgcaaagtc tgcaatcttg attgaggcaa attcacggca aattctcta
//
LOCUS       DAOMTA010000799         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4220_length_1559_cov_1.671543, whole genome shotgun sequence.
ACCESSION   DAOMTA010000799 DAOMTA010000000
VERSION     DAOMTA010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_1559_cov_1.671543"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>286)
                     /locus_tag="PLK68_15780"
     CDS             complement(81..>286)
                     /locus_tag="PLK68_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91910.1"
                     /translation="ETCELFSYPCLLLTQVKYDITGEITKPDDFLKIRINDYKSKEGL
                     AEGITRNINRLIDNLDLNKKFKI"
     assembly_gap    287..296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<297..660)
                     /locus_tag="PLK68_15785"
     CDS             complement(<297..660)
                     /locus_tag="PLK68_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91911.1"
                     /translation="MFLEKIYVVSESGIADVLAVIIPTLTSIIGFIVTYRIAKKQISK
                     ELDADKKRTALENGKEILSYCAKMLDFKWYKSPECLNEINEMMNKVIAYGSRDAIAIL
                     SAYQQYNYTHNFNEKKASQ"
BASE COUNT          512 a          256 c          188 g          593 t
ORIGIN      
        1 atgttcattt ttttcttcaa attttctaat tttcatagtt taacattttt ttatctgtaa
       61 caatcctagc attaaatata ttaaatctta aatttcttat ttaaatcaag attatctatt
      121 aatcggttaa tattcctagt tattccttca gctaatccct ctttactttt gtaatcatta
      181 attctgattt tgaggaaatc atcgggtttg gtaatttcac ctgtaatatc atattttacc
      241 tgtgtcaaaa gaagacaagg ataagagaat aattcacatg tctcttnnnn nnnnnngttg
      301 tgaagctttt ttttcattaa aattatgtgt atagttatat tgttgatatg cagataatat
      361 tgctattgca tctctagaac catatgcaat tactttattc atcatttcat ttatttcatt
      421 caaacattca ggagatttat accatttaaa atcaagcatt ttcgcacaat atgataatat
      481 ctctttcccg ttttccaacg cagttctctt tttatcggca tctaattctt tagaaatttg
      541 ttttttagca atacgatagg tgacaataaa cccaattatt gatgttaaag tgggtataat
      601 aacggctagc acatcagcaa ttccactctc acttacaaca taaatttttt caagaaacat
      661 tttcttttcc ccccctagtt tattttaaat aattatatca cacataccat tataaatcga
      721 cacatgacta ggtaaaactt cctagtcctt ttacatgttg atataaagta ttagcgaatc
      781 gtgaatttca tgaagcaaac taaatttgat ttaagtttac acaaagccca taaatttatg
      841 gatatccaat aagattagag aacctacata aaagcacgga cattcgtcac taatgaagaa
      901 tgggtattac tcaagtcaaa attgatttca gtggatttct gtaattttag aaaacgtaag
      961 attgctttct cataattgag taaccaataa catttatcta gttaaaatat caatataggt
     1021 tcatcatgaa tgtcctaccc cattatataa gaccattcgc catcaggcac aaagggacta
     1081 ttgctcaatt ttgagcaata gatagttata gtcatttttg gctaatacct aaaaaagacc
     1141 aaaggaatac tccatcagtc tttcaaatca attaagcaac taagcattgc ataacatatt
     1201 cttaacagtt cctttttagt taattccata aacttacctc tttcaaggtc cattagatca
     1261 tctttgctag cattcttaaa atatctgttg tagtcaatta ttttcatacg atccacctct
     1321 catggtatta attatactta tgcttatcat aagtatagct tatataaaat ggtaagtaaa
     1381 tataatggcc tagatatagg cttttctttg actgtaggtg tatttaaagt aaattggtat
     1441 tagttatcat attataataa aaagcgctca tacgttaaat atgaggcttt ttattttttg
     1501 cttcattata tgggtaccta gaattaactt cttctatcaa ctcatttttt cttttacta
//
LOCUS       DAOMTA010000800         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4231_length_1556_cov_2.381746, whole genome shotgun sequence.
ACCESSION   DAOMTA010000800 DAOMTA010000000
VERSION     DAOMTA010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4231_length_1556_cov_2.381746"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLK68_15790"
     CDS             <1..199
                     /locus_tag="PLK68_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HRM91912.1"
                     /translation="VAGNVIEPIGRDYLYSELINPIFTKDGDNVKVKVAVKFLDNQTK
                     ATQVSQYELVLQKDSNWKIVE"
     gene            complement(255..617)
                     /locus_tag="PLK68_15795"
     CDS             complement(255..617)
                     /locus_tag="PLK68_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM91913.1"
                     /translation="MEVDYKAIGQRIKIARIKKGVTQETVADLIDITPAHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVITSKIVFEKEAKDILSDCDEYEVRFLVDLMK
                     SAKIAFRKDKDIRNQFQK"
     gene            768..992
                     /locus_tag="PLK68_15800"
     CDS             768..992
                     /locus_tag="PLK68_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HRM91914.1"
                     /translation="MLRFRKKDSKELIKRMAKTNGCSVSEIRREMQYAIDEARNSDDP
                     DKQAEFQRLFGNKIPSPEEFICVVGKNLKF"
     gene            1012..1374
                     /locus_tag="PLK68_15805"
     CDS             1012..1374
                     /locus_tag="PLK68_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stathmin-1-A"
                     /protein_id="HRM91915.1"
                     /translation="MKKAEMQEQEFLEICSSYDYDLLTNKIEMTLKDYQRITYLVGSL
                     GYSQYLQFLIGKYMDLGQADEEQEEREHEIYLEYPEYYEDEGILEEEEKWLEEFLNQL
                     PENKREYFERLIKESQSY"
BASE COUNT          550 a          232 c          283 g          491 t
ORIGIN      
        1 tgtagctgga aatgtgattg aacctatcgg cagggactac ctttattctg aactgataaa
       61 ccctatcttt acaaaggacg gggacaatgt gaaagtcaaa gttgctgtga aattccttga
      121 taatcagaca aaggcaacac aagtatcaca gtatgaactt gtgctacaaa aggatagcaa
      181 ctggaagatt gtagaataaa tgatataaaa agaccgtagc ctgtaataca catttatgtg
      241 ccttacagct acggttattt ttgaaattga ttgcgtatat ccttgtcttt acgaaacgct
      301 attttagcag acttcattaa atctactaag aaacgtactt cgtattcatc acagtctgac
      361 aaaatatctt ttgcttcttt ttcaaaaacg attttagaag taatcacatt atcgcataaa
      421 agagtatcga ctgatacaga aagtgcattg gcaatcgcaa tcaaggttgg taaactgact
      481 tttgtttttc ctgtttctac attgctcatg tgtgctggcg taatgtctat aaggtcggca
      541 acggtttctt gtgttacgcc ctttttaatt cttgcaattt taatacgctg tccgattgct
      601 ttgtaatcaa cttccacagt ttcctctcct ttcctgcttt tttatattgt aaccattcgc
      661 agagtgtaat ataatactct gtataaatga acaattcttt cagtatatat tcaggctaaa
      721 aagtttaaaa tatggaagaa tataaatgca aaggggggta tgtctatatg ttaagatttc
      781 gtaaaaagga tagtaaagag cttataaaaa gaatggctaa aacaaatggc tgttcggtat
      841 ctgaaattcg cagagaaatg cagtatgcta ttgatgaagc tagaaatagt gatgacccag
      901 acaagcaagc ggaatttcaa cgcttatttg gaaataagat acctagtcca gaagaattta
      961 tttgtgtagt tggcaagaat ttaaaatttt agcagatgtg gagcgtatat gatgaaaaaa
     1021 gcagaaatgc aggaacagga atttttagag atttgttctt cttatgacta tgacttatta
     1081 actaacaaaa tagaaatgac attaaaagac tatcaacgaa ttacttattt agttggttct
     1141 ctaggatata gtcaatatct tcaattcctt attggaaaat atatggactt aggacaggct
     1201 gacgaagaac aagaagaaag agaacatgaa atatatttgg aatacccaga gtattatgag
     1261 gacgagggaa ttttggaaga ggaagaaaaa tggctggaag aatttttaaa tcaacttcca
     1321 gagaataaaa gagaatattt tgaaagactt ataaaagaaa gtcaaagcta ctagaaaaat
     1381 aaaaagttac tgtattcaga tgttctgatc acagtaactt tttatcatta tacaagggaa
     1441 aacaaatgct gatagacaaa atatttcctt cgtaacttac attttcaatt ttaccgtcaa
     1501 ttttttttag caattctttt acaatataca agcctaaacc agaacttcct ttgttg
//
LOCUS       DAOMTA010000801         1553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4249_length_1553_cov_1.961949, whole genome shotgun sequence.
ACCESSION   DAOMTA010000801 DAOMTA010000000
VERSION     DAOMTA010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_1553_cov_1.961949"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /gene="aroA"
                     /locus_tag="PLK68_15810"
     CDS             <1..1234
                     /gene="aroA"
                     /locus_tag="PLK68_15810"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRM91916.1"
                     /translation="VPPSKSLAHRAIICASLAKGISRIDNIEYSKDIQATIKAMQSLG
                     TKIEKYDDYLIIDGTTTYTKQNSEIDCEESGSTLRFMVPIAIVEENKAHFVGRGNLGK
                     RPLNTFYEIFERQNIGYLYKEDILDLYVIGKLKPDHYKVPGNISSQFITGLLFALPLL
                     DGDSIIEITSPLESKGYIDLTLQMLNQYGIKIVNNDYKSFIVMGKQEYQAHDYRVEAD
                     FSQAAFYLVAGAIGNDVVLTDLNLDSLQGDKATLEFLEAMGAKISVVSDGIKVTGENL
                     AATIVDASQCPDVIPVVSVALALAQGKSDVINAKRLRIKECDRIIATRSQLNELGGTV
                     TELPDGMTIRGVNEFTGGNCSSFADHRIAMMLAIAATRSSQPVVIDNMECVEKSYPSF
                     WEDYQSLGGIIDVIDVEK"
     gene            1212..>1553
                     /locus_tag="PLK68_15815"
     CDS             1212..>1553
                     /locus_tag="PLK68_15815"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRM91917.1"
                     /translation="MSSTWKNNIEITIFGESHGKAIGIVLGNLPSGIKIDLEEVAKEM
                     KRRAPGQDKMSTARKEADKVEIMSGLQDDVTTGAPLCGIIYNSDQHSKDYSLLKEKMR
                     PGHSDYPAFIKY"
BASE COUNT          517 a          214 c          333 g          489 t
ORIGIN      
        1 agtaccaccg tcaaaatcat tagctcaccg agctattata tgtgctagtt tagctaaagg
       61 aatcagtcgt atcgataata ttgaatattc taaagatatt caagcaacaa ttaaagcaat
      121 gcaatcattg ggaaccaaaa tagagaaata tgatgactat ttaatcattg atggcacaac
      181 tacttataca aagcaaaata gtgagattga ttgtgaagaa tctggatcga cgctaaggtt
      241 tatggttcca attgcaattg ttgaggaaaa taaagctcat tttgttggac gcggtaattt
      301 gggaaaacga ccgctaaata ctttttatga aatatttgag cgtcaaaata ttggttatct
      361 ttataaagag gatatccttg atctatatgt aattggtaag ttaaaacccg atcattataa
      421 ggttcctggg aatatttctt cacagtttat cactggactg ctatttgcat tacctctatt
      481 agatggtgat tcgattattg agatcacttc accgttggaa tcaaaaggat atattgatct
      541 aactttgcag atgttaaatc aatatggaat taaaattgtc aataatgatt ataaaagttt
      601 tattgtaatg gggaaacaag aataccaggc tcatgactat cgagttgagg cagatttttc
      661 tcaggcggct ttttatctag ttgctggagc gattgggaat gatgttgtat taacagattt
      721 gaatcttgat tcgttacagg gagataaagc aactttagaa tttcttgaag ccatgggggc
      781 taaaatttct gtagttagtg atggtatcaa ggttacgggt gaaaacttag cagcaacaat
      841 tgttgacgct agtcaatgcc ctgatgttat acctgtggtt tcagtagctt tagctttagc
      901 tcaaggtaaa agtgatgtta ttaatgctaa aagattgaga attaaagaat gtgatcgaat
      961 catagcaact cgttcacagc ttaatgaatt aggtggaacc gtcacagagt tacctgatgg
     1021 aatgacgatt cgcggtgtta atgagtttac tggcggtaat tgttctagtt ttgcggacca
     1081 tcgaattgca atgatgttag caattgctgc tactcgcagc agtcagcctg ttgttattga
     1141 taatatggaa tgtgtagaaa aatcttatcc aagtttttgg gaagattacc agtcattagg
     1201 aggaattatt gatgtcatcg acgtggaaaa ataatattga gataacaatt tttggagaat
     1261 ctcatggaaa agcaattggg attgttttag gaaatctacc aagcggaatc aagattgatt
     1321 tagaggaagt agccaaagaa atgaaacgtc gtgctcctgg acaggataaa atgtcaactg
     1381 ctcgaaaaga agctgacaag gtagaaataa tgagtgggtt acaggatgat gtaacgactg
     1441 gtgcaccatt atgtggaata atttataata gtgatcagca ttctaaagat tattcattat
     1501 taaaagaaaa aatgcgtcct ggtcatagtg attatccagc ttttattaaa tat
//
LOCUS       DAOMTA010000802         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4253_length_1552_cov_1.931196, whole genome shotgun sequence.
ACCESSION   DAOMTA010000802 DAOMTA010000000
VERSION     DAOMTA010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4253_length_1552_cov_1.931196"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..483
                     /locus_tag="PLK68_15820"
     CDS             268..483
                     /locus_tag="PLK68_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91918.1"
                     /translation="MKISLEEIKKSIYQCIADWEKDGRYNHVAFVVASGSKISSGYDD
                     VTIAQHTSNCCARVTSFVNGWGNTSAV"
     gene            789..>1552
                     /locus_tag="PLK68_15825"
     CDS             789..>1552
                     /locus_tag="PLK68_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM91919.1"
                     /translation="MDELFEKILNHAINYKTSDIHILMKQKCSVSFRQHGDLVLYDTF
                     ESHIGLKLFNYIRFKSNIDINYRLRPQTGHLNYIYQNQIYYLRISSLPGRELDSIVIR
                     ILNNHQQIALDKLTIFKETTVFLKHITTLEAGLFIVSGATGSGKSTTLYTLLDSINAT
                     QQRNIVTLEDPVEIKKEYCLQIQINEKLGITYNNSLKQILRHDPDVIMIGEIRDEQTA
                     RLAVTCALTGHLVLTTIHSSNCLTTIRRLLNLGLLK"
BASE COUNT          591 a          203 c          247 g          511 t
ORIGIN      
        1 gactaataga ataagattca tatttatttt gtgaataaat attatccttt ttattgacct
       61 aaaccttagg gcataatatg taataagcgc aaggagaatg aatgctatga agaccatgaa
      121 gttgaagagt ggtttcatat taccaagaaa gcactcatat attatgaaaa aagttggttt
      181 aataaaacca gatagagatg ataatgacta ccgaaattat agtcaaagcg atgctgatag
      241 tgtgaaactt attgtatttt aagatcaatg aaaatatcac tagaagaaat aaaaaaatca
      301 atatatcaat gtatagcaga ttgggaaaaa gatggacgat acaatcatgt cgcttttgta
      361 gtcgcatctg gaagtaaaat atctagcggg tatgatgatg taacaattgc acaacataca
      421 agtaactgtt gtgctagagt aacctctttc gtaaacggat ggggaaatac ctctgcagta
      481 taaaatttac aatcgaagaa ctacgaatga gaatatagaa attgaaaaat agttacttgc
      541 agattctagt actagataag agtataaaat ataggcgctt taaaataatg tagggtgcct
      601 aacaaaaagt agggttggaa gatgtaatgt cttcattaat aaccctattt tttatgtgtc
      661 tagatatatt atccaattta tattttattt agatttgttt tgcttaaaag gttagaataa
      721 tcgtattttt ttatttttta aaagtaattt ctatattaca ggcgtaaatg ataataggag
      781 gtatttttat ggatgaacta tttgaaaaaa tcttaaatca tgcaattaat tataagacat
      841 cagatattca tattttaatg aagcaaaaat gttctgtttc ttttcggcaa catggtgatt
      901 tggtattata tgatacattt gaaagtcata tcggattaaa attatttaat tatattcgtt
      961 tcaagtcaaa tatagatatt aattatcgtt tacgaccaca aacgggtcat ttaaattata
     1021 tttatcaaaa tcaaatttac tatttacgaa tatctagttt acctggtaga gaattagata
     1081 gtattgtaat tagaatttta aacaatcatc aacaaattgc ccttgataaa ttgactattt
     1141 ttaaagaaac aactgttttt ttaaaacata tcacgacttt agaagccgga ttatttattg
     1201 taagtggagc aactggttca ggaaagtcaa ccacactata taccctacta gattcaatta
     1261 acgctactca acaaagaaat attgtcactc ttgaagatcc ggtcgaaata aaaaaggagt
     1321 attgcttgca aattcaaata aacgaaaaac taggaattac atataataac tctttaaaac
     1381 aaatcttacg ccatgatcct gatgtaataa tgataggtga aatacgcgat gagcaaactg
     1441 ctcgtctagc tgttacatgt gcattaacag gccatttagt attaacaaca atccattcgt
     1501 caaattgcct tacaacaatc agacgtcttt taaacttagg acttttaaaa at
//
LOCUS       DAOMTA010000803         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4262_length_1549_cov_2.400268, whole genome shotgun sequence.
ACCESSION   DAOMTA010000803 DAOMTA010000000
VERSION     DAOMTA010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4262_length_1549_cov_2.400268"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..803)
                     /locus_tag="PLK68_15830"
     CDS             complement(147..803)
                     /locus_tag="PLK68_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91920.1"
                     /translation="MKRVLLFFSLMLIIGTSNTYSQMPVKDKGKWSVLNNMEFMDRVR
                     LQPEWYHYWIWYKKVLGIKIPLPGLGLHDKYGKEDRRNFQLQEIPMMAAVEYNKSETE
                     KEGYNVDTIYRQELFKFGDKEIDYQYTLTKNRRNDILNDINKKLVEYSSNGGNKEHVE
                     VITDEVTRIKKNIDIIHDSHMSNSKKREAYLDFDKELIEVLSLITRLNNINKTIMSHE
                     "
     gene            complement(805..1392)
                     /locus_tag="PLK68_15835"
     CDS             complement(805..1392)
                     /locus_tag="PLK68_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HRM91921.1"
                     /translation="METLIANHKVQHTSFSKVKENEAQISLIQKQISEKMVQIEFFQS
                     KFYNSLKSVEAIIKTGKDIIYCTDIAADIGKYQKQMVELAVGDPALLLVSAKTELELV
                     NRTADLTQYIYQVAIVGTDVNLMDNKQRIDLLKYVINELRNMRGIAYAVCRQMKTAKR
                     NGVLQTLAPGVFKYKDNRSKLVDDLLQNYKVKPKR"
BASE COUNT          452 a          279 c          203 g          615 t
ORIGIN      
        1 tcatcatttt gaagctttct actccaaaat ataacaacat aaagattgct gccaatgctt
       61 gtgcatccgc aataaattct ccgaatagaa cagttgtttt atctcttagt ccatcaaata
      121 attcaaataa gaatttatct attaactcat tcatggctca ttattgtttt atttatatta
      181 ttcaatcttg ttattaatga aagaacctct attagttctt tatcaaaatc taaataggct
      241 tccctttttt tagagttact catatgtgaa tcatggatta tatctatgtt cttttttatt
      301 ctagtaactt catcagttat aacttcaaca tgttctttat taccgccatt agaagaatat
      361 tcgacaagtt ttttatttat atcattaaga atatcatttc tacgattttt agttaaagta
      421 tattgataat caatttcttt atctccaaat ttgaagagtt cctgtctata tatggtatca
      481 acattatacc cttctttttc tgtttctgat ttattgtact ctacggctgc catcatggga
      541 atttcttgta actggaaatt acgtctatct tccttcccgt atttgtcgtg taaccctaag
      601 cccggcaaag gaattttaat acccaaaacc tttttatacc atatccagta atgataccat
      661 tctggttgta gtcgtactct atccataaat tccatattat taagcacgct ccatttccct
      721 ttatccttta caggcatttg ggaataggta ttagaagtac caataatcaa cattagggaa
      781 aaaaataaaa gcactctttt catattatct tttaggtttt actttataat tctgtaataa
      841 atcatcaact aattttgaac gattatcttt gtatttaaat acccccggag ctaatgtttg
      901 taatactcca tttctttttg ctgttttcat ctgtcggcat acagcgtatg ctatacctct
      961 catgtttctt agttcattaa taacatactt aagcaagtct attctttgct tattatccat
     1021 aaggtttaca tctgtcccga caatagcgac ctgataaatg tattgtgtca aatccgcagt
     1081 gcgatttact aattctagct ctgtttttgc tgacaccaat aataaagccg ggtcacctac
     1141 agcaagttca accatttgtt tttgatattt accaatatct gctgcaatat cagtgcagta
     1201 gataatatct tttcctgttt ttatgatggc ttctacagat tttaggctat tataaaattt
     1261 gctttggaaa aattcaatct gaaccatttt ttcactgatt tgtttttgta taaggcttat
     1321 ttgagcttca ttctctttca ctttagagaa agaagtatgt tgcaccttat gattagctat
     1381 aagtgtttcc atacaggtta tatccaagac tgccttttgc gaatatgcaa cttgggttaa
     1441 agtagatgct accactaaaa taaataggat tttcttcatg atattatctt taacggtttg
     1501 taatccattt gtatttatct aggatatact tatataatgg ttgttcccc
//
LOCUS       DAOMTA010000804         1545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4281_length_1545_cov_1.983221, whole genome shotgun sequence.
ACCESSION   DAOMTA010000804 DAOMTA010000000
VERSION     DAOMTA010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_1545_cov_1.983221"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLK68_15840"
     CDS             complement(<1..488)
                     /locus_tag="PLK68_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HRM91922.1"
                     /translation="MKLFKHFKTITKHKFYVMKLCFRFGLYKQGLKHDLSKYSWTELV
                     TGAKYYLGYKSPNSNERDTIGYSSAWLHHKGRNKHHWEYWIDFTSKGIIAIEMPINYV
                     VEMFCDRVAATMVYQGTQFNFKAPLDYYNKTHHYYVINENTDRILRDMLERLANSNLD
                     ET"
     gene            complement(475..1089)
                     /gene="scpB"
                     /locus_tag="PLK68_15845"
     CDS             complement(475..1089)
                     /gene="scpB"
                     /locus_tag="PLK68_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRM91923.1"
                     /translation="MEQNNYLDIIEGMLYLAGDDGVDIKQVAGVLEISKKEATLLMDQ
                     FTEIYGNKALKGIVLVNFGGRYKLATNADYFIYYQKMVEQSSASLSNAALETLAIIAY
                     NQPITRAGVEDIRGVGCDAMIRKLVAKALIKEVGREDTPGMPILYGVTDEFMDTFGLT
                     TLEELPDLADIVEVDEQEDIFATRYREDTSDDISKAESELNETV"
     gene            complement(1089..>1545)
                     /locus_tag="PLK68_15850"
     CDS             complement(1089..>1545)
                     /locus_tag="PLK68_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRM91924.1"
                     /translation="ALIKRLIEYKRYKDVLDEIRDKYEQRQTVITKPAESMDEYVVDT
                     SSMIPEGLEVYDLMKAMQKMFQRKAMMKPLDTHIAKKDISIDERTAQIRNYFKTRVNK
                     KVKFEELFDRHDRFYFIVTFISILVLAKDKEVEIIQDGLFEEIYVEGKV"
BASE COUNT          491 a          314 c          183 g          557 t
ORIGIN      
        1 attgtttcat ctaaattaga attcgccaag cgttcaagca tatctctcaa gattctatct
       61 gtattttcat taatcacata atagtgatgc gttttattat aataatcaag tggtgcttta
      121 aaattaaact gagttccttg ataaaccatc gttgcagcaa cacggtcgca aaacatttca
      181 acaacataat taattggcat ttcaatggcg ataattcctt tgctagtaaa atcgatccaa
      241 tattcccaat gatgtttatt acgtcctttg tgatgcagcc aggcactgct ataaccaata
      301 gtatcacgtt cattactatt tggtgattta tagcccaaat aatatttagc acctgttact
      361 aattcagtcc aagaatattt agataaatca tgttttaacc cttgtttata caaaccaaat
      421 ctgaaacata atttcattac ataaaattta tgttttgtaa ttgttttaaa atgcttaaac
      481 agtttcattt aattcacttt ctgcctttga aatatcgtca ctagtatctt cacgatatcg
      541 tgtagcaaat atatcctctt gttcatccac ttcaacaatg tcagctagat ctggtaattc
      601 ttcaagtgtt gttaaaccaa aagtatccat gaactcatca gttacaccat acaaaatagg
      661 cattcccgga gtatcctctc gcccaacctc tttgatcaag gctttagcta cgagcttacg
      721 aatcattgca tcgcatccaa ctccacgaat atcttcaact ccagcccgtg taatgggctg
      781 attataagca ataattgcta atgtctctaa agcagcattc gataaactag cactgctttg
      841 ttcgaccatc ttttgataat aaataaagta atcagcatta gtagctaatt tataacgtcc
      901 gccaaaatta actagaacaa tcccctttaa agctttattc ccataaattt ctgtaaattg
      961 atccattaat agcgttgctt cttttttact tatttctaat actccggcta cttgtttaat
     1021 atcaacacca tcatcaccag ctagatacaa cataccctca ataatatcta aataattatt
     1081 ttgttccatt aaactttccc ctctacatat atttcttcaa ataacccatc ttgaataatc
     1141 tctacttctt tatctttagc taaaactaat attgaaataa aagttacaat aaaatagaaa
     1201 cggtcatgac ggtcaaataa ttcctcaaat ttaactttct tatttactct tgttttaaaa
     1261 taatttcgta tttgagctgt tctttcatca attgaaatat cttttttagc aatatgtgta
     1321 tccagtggtt tcatcattgc ttttcgctga aacatttttt gcattgcttt cattaaatca
     1381 taaacttcta atccttcagg aatcattgaa ctagtatcca caacatactc gtccattgac
     1441 tctgccggtt tagtaataac tgtttgacgc tgttcatatt tatcacgaat ttcatctaaa
     1501 acatctttat aacgcttata ctcgattagt cgtttgatca aagct
//
LOCUS       DAOMTA010000805         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4291_length_1544_cov_1.263264, whole genome shotgun sequence.
ACCESSION   DAOMTA010000805 DAOMTA010000000
VERSION     DAOMTA010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_1544_cov_1.263264"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PLK68_15855"
     CDS             <1..1454
                     /locus_tag="PLK68_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HRM91925.1"
                     /translation="TGIGLVTKGKVNVSTMKSLLTSKGDTNRAAKACDCATVLNKQQA
                     LDIKNAGYTHVGRYLTGSVGKEHTPKYLTSTEVKNIENAGLSVFPIYQDGGYELNYFK
                     DPSQGSVDAQTAILAAERIGIPSGTTIYFAVDFDCYSYQIDTFIIPYFEQIHMIFFSS
                     TNDKNYKVGIYAPRYVCTKVYEAGLASKSFVADMSTGFSCNLGYSMPKNWAFDQFCEL
                     NSFSSSPSFPLDKDAYSGRDTGFKKFDAVSTKTDEEIAQENLRAKVKIARNQYVYNVM
                     EPLGYLNKIMDVGVEYDKEISLGTMMSPQGAIDISTKISTSLESSTGKIYNIKVDIGN
                     DGELTQTCKNQIMEISSNLSDTGIEGADNFGNTIEKIALSVKSGNIAFEINNVFANSV
                     EFSIVFSTSDLLPEEEKEWTISVALIFTMTLNSNSGLEFNVVEFTKEHSNILAGAVIL
                     VLAGALVVNAIPSIIALFSAGAGTVFGLLIQAL"
BASE COUNT          542 a          204 c          334 g          464 t
ORIGIN      
        1 tgacaggaat tggacttgta acaaaaggaa aagtaaatgt ttctactatg aaatcattgc
       61 ttacgagtaa aggcgataca aacagggcag caaaagcgtg tgactgtgca acagtattaa
      121 ataaacaaca ggcattagat ataaaaaatg cagggtatac tcatgtagga cgttatctga
      181 caggttctgt tggaaaagaa catacaccga aatatttgac atcaacagaa gtgaaaaaca
      241 ttgaaaatgc aggattatcg gtattcccga tatatcagga tggaggatat gaattaaact
      301 attttaagga tccgtcccag ggaagtgttg atgcacagac agcaatttta gcagccgaga
      361 gaatcggtat accaagtgga acgactatat actttgcagt ggattttgat tgttattcat
      421 atcagattga tacatttatt ataccttatt ttgaacagat tcacatgatc tttttcagtt
      481 cgacaaatga taaaaattat aaagtcggca tttatgcacc aagatatgta tgtactaaag
      541 tttatgaagc aggattggca agtaaatcat ttgtggcaga tatgtcgact gggtttagct
      601 gtaatttggg atattcgatg ccgaagaact gggcatttga tcaattttgt gagttgaact
      661 cattttcttc atcaccttcg tttccgctgg ataaagatgc atattcaggg agagatactg
      721 gatttaaaaa gttcgatgcc gtatcgacaa aaacagatga ggaaatagcg caagaaaatc
      781 ttcgggcaaa ggtaaaaata gcacgaaatc aatatgtcta taatgtaatg gagccgcttg
      841 gttatctgaa taaaattatg gatgtgggtg tggagtatga taaggaaatt tcactgggaa
      901 caatgatgag tcctcaagga gcaattgata tttctactaa aatatccaca tcgcttgaaa
      961 gttcaactgg taaaatttat aatataaaag tggatatagg aaatgatgga gaattgacac
     1021 aaacttgcaa aaatcaaatt atggagattt catcaaattt atcagataca ggtatagagg
     1081 gagcagataa ctttggaaat acgatagaaa aaattgcttt atcggttaaa tctggtaata
     1141 ttgcttttga aataaataat gtttttgcca atagtgtaga gttttccatt gtatttagta
     1201 catcagattt gctgccagaa gaggaaaaag agtggacaat atcggttgct ttgattttta
     1261 caatgacatt aaattctaat agtggtcttg aatttaatgt tgtagaattc acaaaagagc
     1321 atagtaatat actggcagga gccgtaatat tagttcttgc gggtgcatta gttgtaaatg
     1381 caattccgtc aattattgct ttattttcag ctggagcagg tactgttttt ggtttgttga
     1441 ttcaagcgct gtaaattgta aaagaataaa agagacaatg gccagaggtt cgattttata
     1501 aagaggacct ctggcaaaag ggggattaag aagaatgcat atta
//
LOCUS       DAOMTA010000806         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4296_length_1542_cov_2.516476, whole genome shotgun sequence.
ACCESSION   DAOMTA010000806 DAOMTA010000000
VERSION     DAOMTA010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_1542_cov_2.516476"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLK68_15860"
     CDS             complement(<1..1109)
                     /locus_tag="PLK68_15860"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRM91926.1"
                     /translation="MKLRKITKKTVSIIIAFTFMLGFTDGLKLNAANNISDETNYSPT
                     TKVLDDGSIVELNENDYNSSGYVITKQRSVDLSEGVVNFRTKSSNSNTTYVEDGTNKS
                     GYTNGTYAADAAFLGYNSDKSKIKFMLSGVIGWVNSSEVQLLSYSDSAVQTLSKYEVS
                     NGTIYHEISNNLNNSSYSSKINVGKIGLPDGLYYSYDGHYFYSYGRNGYRTMINDYKN
                     NVRSNAVNANQPYYNYFQYLPHRSLTNYSGSQINEFIKLNTGSTSKLQGTGNAFIQSQ
                     NKYGSNALLMLGVAINESAWGNSNIAMKKNNLFGHGATDNNPFWGSNGYNDPGDSIMY
                     HGGYFISRGYCYPRDWRYNGSHLGDKASGVNVQYAS"
     gene            complement(1123..>1542)
                     /locus_tag="PLK68_15865"
     CDS             complement(1123..>1542)
                     /locus_tag="PLK68_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91927.1"
                     /translation="GYRNMPLCEDYDFTLRAILEGYRISNINIAVLNYRMTKNSVSRS
                     NLFEQYLFARYITSQYSKGLVASIDDAHNYVKKHNNKKDSDKYLQANIYFNKALNDIE
                     EKKYIRLIKDGILLLFSSRYYLNKVYRFIRVSLCSNK"
BASE COUNT          498 a          283 c          169 g          592 t
ORIGIN      
        1 gaagcatatt gaacattcac accactagct ttatcaccta aatgtgatcc attgtatcgc
       61 cagtctctag gataacaata ccctcttgag ataaaataac caccatggta cataatacta
      121 tccccaggat cattataacc attagaaccc caaaatggat tattatcggt agccccgtgt
      181 ccaaataaat tatttttttt catagcaata ttactatttc cccacgcact ttcattaatt
      241 gccacgccta acattaaaag tgcatttgaa ccatatttat tttgactttg gataaatgcg
      301 tttcctgtgc cttgtagttt agatgtacta ccagtattca atttaataaa ttcgtttatt
      361 tgacttcctg aataatttgt taaacttcta tgtggtaaat attgaaaata attataatac
      421 ggttgattag catttacagc attagatctt acattatttt tataatcatt tatcattgtt
      481 ctataaccgt ttcgtccata actataaaaa taatgaccat cataactata atataatcca
      541 tctggtagac caattttacc tacattgatc tttgatgaat atgaggagtt attcaaattg
      601 ttcgatattt catggtatat tgttccatta cttacttcat acttacttaa tgtctgtact
      661 gcagaatccg aatatgataa taattgtact tctgatgaat ttacccagcc aataacccct
      721 gaaagcataa atttaatttt acttttgtca gaattatacc ctaaaaacgc tgcatctgct
      781 gcataagtac cattcgtgta cccagattta tttgttccat cttccacata tgtagtattt
      841 gaattcgatg attttgtcct aaaattaaca acgccttcac ttaaatcaac acttctttgt
      901 ttagttataa catatccaga cgaattgtaa tcattctcat ttaattctac aatactccca
      961 tcatctaaaa ccttagttgt tggagaatag ttagtctcgt cacttatatt attagccgca
     1021 tttaatttaa gaccatccgt aaatcctagc ataaaagtaa atgctattat tattgatact
     1081 gttttttttg ttattttcct taatttcatc ctataccacc ttttatttat ttgagcataa
     1141 gctcactctt ataaatctat atactttatt taaataatat cgtgatgaaa acaataatag
     1201 aattccatct tttattagtc taatatactt cttttcttct atatcgttca gcgccttatt
     1261 aaaataaata tttgcttgta aatatttatc agaatctttc ttattattat gcttctttac
     1321 ataattatga gcatcatcaa tgcttgctac taatcctttt gaatactgtg atgtaatata
     1381 tcttgcaaag aggtattgtt caaataaatt tgatctcgac acactattct tagtcattct
     1441 gtagtttaaa acagcaatat taatattaga aatcctatat ccttctaata ttgcccttaa
     1501 agtaaaatca taatcttcac ataaaggcat atttctatat cc
//
LOCUS       DAOMTA010000807         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4302_length_1541_cov_2.821669, whole genome shotgun sequence.
ACCESSION   DAOMTA010000807 DAOMTA010000000
VERSION     DAOMTA010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4302_length_1541_cov_2.821669"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1453)
                     /locus_tag="PLK68_15870"
     CDS             complement(200..1453)
                     /locus_tag="PLK68_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HRM91928.1"
                     /translation="MDILNIDNRELCQILNSILFGFLLLWYTKKNKKGKFNQTTLTLS
                     IWFFSSISGILYVFSENSYIGQGKANFYYVFLIFLLFLLFSWPLISLKERTLESYDYD
                     NSNFLKFTCIFISIISILPFTENVIHLFSGNTFEEMGAYHDELIVKSSAKHLSWLGIR
                     LTSIMSWFRYLTPVLFFNYINQKNTNKNKYIICGLLIALINNSVNSFSIGARNIVVQE
                     MSQLLFLYLFMRKTINPKLRKQIVKLSTGFILILVLGITIITIYRFSNSTNETLEESI
                     FRYSGEGFNNLYTDMLYVKTHTYGLHIFRTIFNMTPESLSAITGVRMFVFYTFIGDFI
                     ADFDIFGTIIIFSFTSIIIYHTNIRSKKSGFLSLSILSIYADIIISGYMYVPFMNYST
                     AIPFFLGYIIFYSFISKKKVVSSQK"
BASE COUNT          594 a          222 c          208 g          517 t
ORIGIN      
        1 ttttgtacca tttgtaaaac tcattatagt tcacatcatt gttcatgcag aactgatcga
       61 tcgaaatgtt acgaggagct ccttccacct ggtacaacat ccacagaccc tccattaatc
      121 tgtctttttc ttctttcatt ttgcgataat ttaaaattga ttcgcaaaaa taataattag
      181 aaatcattta cacaagacgt cacttttgac tgcttacgac ttttttttta gaaataaagg
      241 aataaaaaat tatatatcca agaaagaatg gtattgctgt agagtaattc ataaatggta
      301 catacatgta acccgatata attatatcag cgtatatgga taatatacta agtgaaagaa
      361 aacctgattt tttactccga atatttgtgt gatatattat tattgatgta aaagaaaaaa
      421 ttataatagt accgaaaata tcgaaatcag cgataaaatc acctataaaa gtgtaaaaaa
      481 caaacatacg tactcctgtt atggcagaca gagactcagg tgtcatatta aatatagttc
      541 taaaaatatg caggccatat gtatgagttt tgacatacaa catatcagta taaagattat
      601 taaaaccttc accgctatat cggaaaattg attcttccaa agtttcattt gttgaattac
      661 taaatctata gattgtaatt atagtaattc ccaaaactaa aataagaata aagccagttg
      721 ataatttgac aatttgtttt cttaacttcg gattaattgt ttttctcata aacaaatata
      781 aaaaaagaag ttgagacatt tcttggacta caatattcct agctccaatc gaaaaagaat
      841 ttacggaatt gtttattaaa gcaatcaaca aaccacaaat aatatattta tttttatttg
      901 tattcttttg attgatgtaa ttaaagaata aaacaggtgt aagatatcta aaccagctca
      961 tgatgctggt taaacgaatg cccaaccaag ataaatgttt tgctgaagat tttacaatta
     1021 actcatcatg ataagcaccc atttcttcaa aagtatttcc actgaaaagg tgaatgacgt
     1081 tttcagtaaa aggtagtata cttattatgg atataaaaat acatgtaaat tttaaaaaat
     1141 tactattatc atagtcataa ctttctaatg tcctttcttt tagcgaaatt aatggccatg
     1201 aaaataataa aaataataaa aatattagga aaacgtaata aaaatttgct ttgccttgac
     1261 caatataact attttcacta aatacatata gaatgcccga tatagaagag aaaaaccaga
     1321 tagacaatgt aagggttgtt tgattaaatt ttcctttttt gtttttcttg gtgtaccata
     1381 gaagcaggaa gccaaacaat attgaattta atatttggca tagttctcta ttatctatat
     1441 taagaatatc catcttaaat aatttacatt aaaaattttg aaacgaaagc agtcaaaagt
     1501 gacgttcccc ctataaaaga atgtttaaaa gcaggtgtat c
//
LOCUS       DAOMTA010000808         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4322_length_1537_cov_2.016194, whole genome shotgun sequence.
ACCESSION   DAOMTA010000808 DAOMTA010000000
VERSION     DAOMTA010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_1537_cov_2.016194"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>760
                     /locus_tag="PLK68_15875"
     CDS             120..>760
                     /locus_tag="PLK68_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetylesterase"
                     /protein_id="HRM91929.1"
                     /translation="MKLRLAEIFTSRMVLQHGKEINIFGTGETNQQVTITIQGQTATT
                     TIINNNWLITLPALEISTDETLIVSTGLETITLSNILIGDVYFLAGQSNIEFKFKDCD
                     SVKVDQEVCYDRYLRYYEVPQIEYEDENIKIPPIRNQGWQICDNQTINDFSAIGYYLA
                     YYLRHDIDIPIGLIAVNKGGTSGSCWINETYLQKNQEIKKVYYDEYYQAIMNQT"
     assembly_gap    761..770
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <771..>1537
                     /locus_tag="PLK68_15880"
     CDS             <771..>1537
                     /locus_tag="PLK68_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRM91930.1"
                     /translation="EAQEDLEIAKYKERVKQYQQKVALYQQTYPERNMSQLKKDVGHT
                     PWPGPRGKKDFCRPAGLYYTMFKKICQYSGKAVIWYQGEEDTKNAYLYHQLLQLVIEN
                     WREDMKAQIPFIIVQLPEYDDDKNDNWPILRDAQQQVCREVPACYLVVTMNCGEEFNI
                     HPQNKKTVGHRIFWRIKELFYDNQFNGHSPKIINIENNSKIIIEFDQKLHSRGINNFI
                     LELPDHQVETVGIIKENKLLVERPAQVRTISYGYQNY"
BASE COUNT          596 a          200 c          284 g          447 t
ORIGIN      
        1 ggcaatatat tacccatttg ttaaaatagc tgataaaatg gaattagata aaatgactga
       61 aaccgagaaa ttagaagcgg tggagacaaa aagagtaagt ttattaagag gcagaaaaaa
      121 tgaaactaag attagctgaa atctttacat cgagaatggt tttgcaacat ggcaaagaaa
      181 taaatatttt tggaaccggt gaaacaaatc aacaagtaac tattactatt caaggacaaa
      241 ccgcaacaac aacaataata aataataatt ggttgattac gctgccggcg ctagaaataa
      301 gtacggatga aacattaata gtttcaactg gattagaaac tattacgtta tcaaatattt
      361 taattggaga tgtttatttt ttagcaggtc aatctaatat tgaatttaaa tttaaagatt
      421 gtgattcagt aaaagttgat caggaagtat gctatgatcg atatttaagg tattatgaag
      481 ttccacaaat tgaatatgag gatgaaaata tcaaaatacc gcccattaga aatcaaggct
      541 ggcaaatatg tgataatcaa acgattaatg atttttcagc aatcggttat tatcttgctt
      601 attatttgcg tcatgatata gacatcccaa tcggattaat tgcagttaat aaaggtggta
      661 catcaggttc ttgctggata aatgaaacat atcttcaaaa aaatcaagaa atcaaaaaag
      721 tatactatga tgaatattat caagcaatca tgaatcaaac nnnnnnnnnn gaagcacaag
      781 aagatttaga aatagctaaa tataaagagc gtgttaagca gtaccagcaa aaagtggcat
      841 tatatcagca aacttatcca gaacgtaata tgagccagtt aaaaaaggat gttggacata
      901 caccatggcc gggaccgcgg gggaaaaaag atttttgtcg tccagcagga ctatattaca
      961 cgatgtttaa aaaaatctgt caatatagtg gtaaggcagt tatttggtat caaggggaag
     1021 aggatactaa aaatgcttat ttatatcatc aattgctaca actggtgatt gaaaattggc
     1081 gtgaagatat gaaagctcaa attcccttta ttattgtgca attacctgaa tatgatgatg
     1141 ataagaatga taactggcct attttaagag atgcccagca gcaggtttgt agagaagtac
     1201 ctgcttgcta tttagttgta acaatgaatt gcggggaaga atttaatatc catccccaaa
     1261 ataaaaaaac agtcggtcat cgtatttttt ggcgaatcaa agaacttttt tatgataatc
     1321 aatttaatgg gcattctccg aagataataa atattgaaaa taattctaaa attataattg
     1381 agtttgatca aaaattacac agtagaggta ttaataattt tattttagaa ttaccagatc
     1441 atcaagttga gacagttggg ataattaaag aaaataaact gctggttgag cgacctgccc
     1501 aagttagaac aattagttat ggatatcaaa attattg
//
LOCUS       DAOMTA010000809         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4327_length_1536_cov_2.596894, whole genome shotgun sequence.
ACCESSION   DAOMTA010000809 DAOMTA010000000
VERSION     DAOMTA010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_1536_cov_2.596894"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLK68_15885"
     CDS             <1..873
                     /locus_tag="PLK68_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM91931.1"
                     /translation="DSSGNVNEKTFTFAVSDLTAPVVTLSQGNDIVIDYGSEFKLENF
                     LTATDDQSAVTNTVTGEVDTKKENEVQTITVSTQDEAKNEVLTTLNFTVKDISGPQVN
                     LSTNAVEVIKGDAFDPRQYLVSAIDNKDGDVTGNVVIGNIDTGSTGDKAVTYTVSDSS
                     GNQTVATLNVKVYTPGSKILETAYTKLGSPYVWGATGPNSFDCSGFTSWVYRQHGISL
                     SRTAQAQSQGGKAVDRADLQPGDLVFFGSSTSRITHVGIYVGNGQMVHSPQTGDVVKV
                     SSLNRNYVCARRYL"
     gene            937..1410
                     /locus_tag="PLK68_15890"
     CDS             937..1410
                     /locus_tag="PLK68_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HRM91932.1"
                     /translation="MNSFDLIRVLCNTSPKAYALITGATVTGTMLAYSFEEGTIVVVE
                     AQGLPATGCGLGVHGLHIHEGSSCSGTPENPFGNAGGHYSTTNCPHPYHTGDLPPLFS
                     EDGQAWMAVYISKFTPEQIVGRTIIIHGNVDDFTTQPSGNSGPMIACGVIRELYQ"
BASE COUNT          492 a          210 c          338 g          496 t
ORIGIN      
        1 gattcttctg ggaatgtaaa tgaaaagacg tttacttttg cagtatctga tttgactgct
       61 ccagtagtta ctttatcaca aggaaatgat attgtaattg attatggtag cgaatttaaa
      121 ttagagaact ttttaacagc tactgatgat caaagtgctg ttaccaacac tgttactggt
      181 gaagtagaca ctaagaaaga aaatgaagta caaacaatta ctgttagtac gcaagatgaa
      241 gctaaaaatg aagttttaac aactttgaat tttacagtaa aagatataag tggaccacaa
      301 gttaatttgt caacaaatgc agttgaggta attaaaggtg atgcttttga tccaagacaa
      361 tatttagtct cagctattga taataaagat ggtgatgtga ctggtaatgt tgtaatcggt
      421 aatattgata ctggcagcac aggagataaa gctgttactt atacagtaag tgatagttct
      481 ggtaatcaaa cagttgcaac tttaaatgta aaggtttata caccgggaag taaaatttta
      541 gaaactgctt atacaaaact aggtagccca tacgtatggg gagccacggg accaaattca
      601 tttgactgtt caggatttac atcttgggta tatcgtcagc atggaatttc cctttcaaga
      661 actgcgcaag ctcaatctca aggtggtaaa gcagttgatc gtgcagattt acaaccagga
      721 gatttagtct tctttggttc aagtacaagt agaattactc acgttggaat ttatgttgga
      781 aatggacaaa tggttcattc accacaaact ggagatgttg taaaagtgtc tagtttaaat
      841 cgtaattacg tgtgtgcaag aagatattta tagaaaaggg cgtttgtcct tttctttttt
      901 ttatatttag gcataagaat ataggggagg tatataatga atagttttga tcttattagg
      961 gtattatgta atactagtcc taaagcttat gctttaatta caggagcaac agttacggga
     1021 acaatgctag catatagttt tgaagaagga acgatcgttg tggttgaggc tcaagggtta
     1081 ccggctaccg gttgtggttt aggggtacat ggtttgcata ttcatgaagg ttctagctgt
     1141 agtgggacac cggaaaatcc ttttggtaat gctggaggac attatagtac gacaaattgt
     1201 ccacatccat atcatactgg ggatctgcca cctttatttt ctgaggacgg acaagcttgg
     1261 atggcggtat atatttctaa atttacacca gagcaaattg ttgggcggac tattataatt
     1321 catggaaatg ttgatgattt tactactcag ccatcgggaa attctgggcc gatgattgct
     1381 tgtggtgtga ttagggaatt atatcaataa tatttacttt tcttaagtta aaaaacaatc
     1441 taactatggt acaatgtagt ggcggaggta tgaagatgag taaattatta ttttttgata
     1501 tcgatggaac actgattgag tgtaatttag atatct
//
LOCUS       DAOMTA010000810         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4329_length_1535_cov_2.315541, whole genome shotgun sequence.
ACCESSION   DAOMTA010000810 DAOMTA010000000
VERSION     DAOMTA010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_1535_cov_2.315541"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLK68_15895"
     CDS             <1..306
                     /locus_tag="PLK68_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM91933.1"
                     /translation="IENGAIFVTRSGRPLDRSNIWKAMKDLCDDAKVERSKVYPHNLR
                     HLFAVTYYNLKKDIARLADLLGHSSMDTTRIYTMSSGREFKRYFDQMDLVFSNRKNN"
     gene            624..863
                     /locus_tag="PLK68_15900"
     CDS             624..863
                     /locus_tag="PLK68_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91934.1"
                     /translation="MEKEELLGQLRNITNCMIISDLRYVGKDVLYSAILSLNVDDYSM
                     KEWHDAIIYLTGNDVDKSIGKAAAKEYLCDYYRHN"
     gene            complement(892..>1535)
                     /locus_tag="PLK68_15905"
     CDS             complement(892..>1535)
                     /locus_tag="PLK68_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRM91935.1"
                     /translation="VDQWDWEKVIAKEERTEETLKRHVKKIFKVIKHMEHEVWYKYPH
                     AVNRLPDKIHFFTSQELEGRYPDLTPNERETAICKELGCVFVMGVGCKLKSGIKHDGR
                     APDYDDWNLNGDILFWFEPLQCALEISSMGIRVDEDTLVKQLEAENCLDRLKLPYHSQ
                     IVNKELPYTIGGGIGQSRLCMLLLKKVHVGEVQASIWPQEMIDECTKHNINLL"
BASE COUNT          508 a          219 c          235 g          573 t
ORIGIN      
        1 attgaaaatg gggcaatatt tgtaaccaga agcggaagac cgttagatcg cagtaatatt
       61 tggaaagcga tgaaagattt gtgtgatgat gctaaggttg agcgcagtaa agtttatcct
      121 cataatttac gtcatctttt tgcggttact tactataatt taaaaaaaga tattgcacgt
      181 ttagcagatt tattaggaca ttcaagtatg gacactactc gtatttatac aatgtcaagt
      241 ggtcgtgaat ttaaaagata ttttgatcag atggatctag tattttctaa ccgaaaaaat
      301 aattaaaata acacgtatta tgtggtattt gattaacaag aactattctt ccgaatatca
      361 ttttacatca ttaagttaat tttaacaagg aatttgaaat attattaaca gcatttttat
      421 gggaatctcg gacaattatt ttcttttttt ctaaaatata tattttttaa tatttttcaa
      481 aatttattca cacttaaaag aacctcaaat ttttaatttt ttaaggttgt ttttgcagtg
      541 tttatttttt atagtagttt accacataat acgtgttatg tggtaaataa tatatattta
      601 ataagatatt gaggggtaca gaagtggaaa aagaagagtt attaggtcaa ttaagaaata
      661 ttacaaattg tatgattatt tctgatttaa ggtatgttgg taaagatgta ttgtattctg
      721 caattctgtc tttaaatgtt gatgattata gtatgaaaga atggcatgat gcaattatat
      781 atttaactgg aaatgatgta gataaatcaa ttggtaaagc tgctgcaaaa gaatacctat
      841 gtgactatta tcgtcataat taaaaaacat tggagtatct tccaatgttt tttatagtaa
      901 attaatattg tgtttagtac attcatcaat catttcttgt ggccaaatac ttgcctgaac
      961 ttctccaaca tgaacttttt tcaataataa catacatagt cttgattgcc cgattccacc
     1021 accaattgtg tatggtaatt ctttgttaac gatttgacta tgataaggga gtttaagacg
     1081 atctaaacaa ttttcagctt ctaattgttt tacaagtgta tcttcatcga ctctaattcc
     1141 cattgaagaa atttctaatg cacattgtaa tggttcaaac cagaataaaa tatccccatt
     1201 taaattccaa tcatcatagt caggagcccg accatcatgt ttaattccac tttttaattt
     1261 acagcctacg cccattacaa aaacacatcc taattcttta caaatcgctg tttctctttc
     1321 gttaggagtt aaatcggggt aacgaccttc taattcttga gatgtaaaga aatgaatttt
     1381 atcaggaagg cggtttacag catgtggata tttataccat acctcatgtt ccatatgttt
     1441 gattacttta aaaatctttt taacatgacg ttttaaagtt tcttcagtac gttcttcttt
     1501 agcgatcact ttttcccaat cccattgatc tacgt
//
LOCUS       DAOMTA010000811         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4336_length_1534_cov_1.872211, whole genome shotgun sequence.
ACCESSION   DAOMTA010000811 DAOMTA010000000
VERSION     DAOMTA010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4336_length_1534_cov_1.872211"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="carB"
                     /locus_tag="PLK68_15910"
     CDS             complement(<1..1121)
                     /gene="carB"
                     /locus_tag="PLK68_15910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM91936.1"
                     /translation="MPKDSRIKKVLVIGSGPIIIGQAAEFDYAGTQACRALKEEGIEV
                     VLINSNPATIMTDKDIADKVYIEPLTVPVVKSLIIKEQPDSILPTLGGQNALNIAMAL
                     ADEGFLEEHNVQTIGTSTNTIKLAEDRLEFKTLMENINEPCAASLVVNHVDDALEFAK
                     KIGYPVVVRPAYTLGGTGGGIAYDETELHDICSNGLRLSRVSQCLIERCIAGWKEIEY
                     EVMRDGAGNCITVCNMENLDPVGVHTGDSIVVAPSQTLSDKEYQMLRTSALNIITALD
                     IQGGCNVQYALNPDSFEYCVIEVNPRVSRSSALASKATGYPIAKLAAKIALGYTLDEI
                     INAVTGKTYASYEPTLDYVVCKIPKWPFDKFVYAKRKLGT"
     gene            complement(1123..>1534)
                     /locus_tag="PLK68_15915"
     CDS             complement(1123..>1534)
                     /locus_tag="PLK68_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793045.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRM91937.1"
                     /translation="GDPEENVQIIEEIKLLASSQTPIFAICLGHQLMALAHGFKTEKM
                     KYGHHGANHPVKDLETGRVYISTQNHNFVIKEDSIDSAVAKPWFINVNDKTIEGVTYL
                     KENIKTVQFHPEACAGPLDTDALFEEFIKMMEGK"
BASE COUNT          472 a          318 c          218 g          526 t
ORIGIN      
        1 gtgcctaatt tacgcttagc ataaacaaat ttatcaaatg gccatttagg aattttacat
       61 acgacataat ctaatgttgg ttcatatgaa gcataagttt tccctgttac tgcattgatg
      121 atttcatcta atgtgtaccc taaagcaatc tttgctgcta gttttgcaat tggataacca
      181 gtagctttac tagctaaagc tgaagaacga ctaactcgtg gatttacttc aattacacag
      241 tattcaaaac tatcgggatt tagcgcatat tgaacattac acccaccttg aatatctaat
      301 gctgtaataa tatttaaagc agaggttctt aacatttgat attctttatc tgataaagtt
      361 tggcttggcg ctacaacaat actgtcacca gtatgaactc ctacaggatc taaattttcc
      421 atgttgcata cagtgatgca gtttccagca ccatcacgca ttacttcata ctcaatttct
      481 ttccatccgg caatacacct ttcaattaaa cattgactaa ctcgtgataa ccttaatcca
      541 tttgaacaga tatcatgtaa ttctgtttca tcataagcga ttccgcctcc cgttccacct
      601 aaagtataag caggacgtac tacaactgga taaccaatct ttttagcaaa ctctaaagca
      661 tcatcaacat gattaacaac taatgaggct gcacatggtt cattaatatt ttccattagg
      721 gttttaaact ctaagcgatc ttcggctaat ttaatcgtat tagttgaagt cccaatcgtt
      781 tgaacattat gttcttctaa aaaaccttca tcagctaatg ccatcgcaat attcaaagca
      841 ttttgaccac ccagggttgg caaaatactg tctggttgtt ctttaattat taaactttta
      901 acaaccggta cggttaaggg ctcaatataa accttatcag caatatcctt atcagtcatg
      961 attgttgcag ggttagagtt gattaaaact acttcaatcc cttcttcttt taatgcccga
     1021 caagcttgag ttccagcata atcaaattct gctgcttgac caataataat tggtcccgaa
     1081 ccaattacta atactttttt tattctacta tcttttggca ttctattttc cctccatcat
     1141 ctttataaat tcttcaaata aagcatcagt atccaatgga ccagcacatg cttcagggtg
     1201 aaattgtact gttttaatat tttctttaag ataagtaaca ccttcgattg tcttatcatt
     1261 tacattgata aaccatggtt ttgctactgc tgaatcaatt gaatcttctt taatcacaaa
     1321 attatgattc tgagtagaaa tatatactcg accagtttct aaatctttaa ccggatgatt
     1381 agcaccatga tggccatact tcatcttttc agttttgaaa ccatgggcta aagccattaa
     1441 ctgatgtcct aaacaaatcg caaaaatcgg tgtttgacta cttgcaagca gcttgatttc
     1501 ttcaataatt tgaacatttt cctcaggatc tcca
//
LOCUS       DAOMTA010000812         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4351_length_1530_cov_2.145085, whole genome shotgun sequence.
ACCESSION   DAOMTA010000812 DAOMTA010000000
VERSION     DAOMTA010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4351_length_1530_cov_2.145085"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLK68_15920"
     CDS             complement(<1..1332)
                     /locus_tag="PLK68_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRM91938.1"
                     /translation="MRGIYSSLTKIRRKVFVEVAKLAYEGNDYSRIENLPYKVIPGEV
                     ATYRDSIFLERAIVGERIRLTMGLSLRPIDEPAPISKGIEESIIADKYYEPPLINIIK
                     FACNKCPEKKFEVSSGCQACLAHPCIEVCPKNAISFKDGKAYIDQDKCIKCGLCKTNC
                     PYDAILKRERPCAKACGMDAIETDEYGNAHINYDKCVSCGMCLVSCPFGAIADKSQIF
                     QLIQAIKAGDEIIAAVAPAFIGQFGPKVTPEVLKKAMQQLGFKDVGEVAIGADLCTID
                     EAKDFLEKVPAQQPFMATSCCPSWSMMAKKEFPEFKSYISMALTARLIKEKHPNSRVV
                     FVGPCAAKKLEASRKSVRSEVDFVLTFEEIGAMFEAKGIDFASLKPDEADPFTQASSD
                     GRGFAVSGGVAKAVVNCIKAKEPEREVLVESAEGLANCKKMLKLAKSGKYDG"
BASE COUNT          480 a          309 c          232 g          509 t
ORIGIN      
        1 accgtcatat ttaccacttt ttgctaattt aagcattttt ttacaatttg ctaatccttc
       61 tgctgattcc actaatactt cacgttcagg ctctttagct ttaatacaat ttacgactgc
      121 tttagcaacc ccaccgctaa cggcaaaacc gcgaccatca ctactagcct gagtaaatgg
      181 atcagcttca tctggtttta aagaagcaaa atcaatgcct ttagcttcaa acattgcgcc
      241 aatttcttca aatgttaaaa caaaatcaac ctcacttcgt actgattttc tagaagcttc
      301 tagttttttt gctgcacatg gccctacaaa aacaactcgg ctattaggat gtttttcttt
      361 tatcaaacga gcggttaaag ccattgatat ataagattta aattccggga attctttttt
      421 ggccatcatt gaccaagaag gacaacatga tgttgccata aacggttgct gagcaggtac
      481 tttttctaaa aagtcctttg cttcatcaat tgtacataaa tcagccccaa tggcaacctc
      541 acccacatcc ttaaaaccta attgctgcat tgcttttttt aaaacttctg gtgtgacttt
      601 aggaccaaat tgtccaataa atgcaggagc tactgctgca ataatttcat caccggcctt
      661 aattgcttga attaattgga aaatttgact tttatcagca atggccccaa agggacagct
      721 aactaagcac attccacaag agacgcattt atcataatta atatgggcat taccatattc
      781 atcagtttca atagcatcca ttccacaagc cttagcacat ggtctttctc tttttaaaat
      841 tgcatcatat ggacaattag ttttacaaag accacattta atacatttat cttgatcaat
      901 atatgctttc ccgtctttaa agctgattgc atttttagga caaacttcga tacatgggtg
      961 cgctaaacag gcctggcagc cactactgac ctcaaatttc ttttctggac atttattaca
     1021 ggcaaattta ataatgttga ttaatggtgg ttcgtaatat ttgtcggcaa taatactttc
     1081 ttcaatccct ttagatattg gtgctggttc atcaataggg cgcaatgata atcccatcgt
     1141 taaacgaatt ctttcaccga caatagctcg ttccaaaaaa atactgtcac gataagttgc
     1201 gacttctcca ggaattactt tatagggtaa attttcgata cgactataat cattcccctc
     1261 ataggctaac tttgcaactt caacaaaaac tttacgtcga attttagtta atgatgaata
     1321 aattcctctc atagtgtttc ctccatatta tttataacta tattttactc caaagtgcaa
     1381 gtttataaaa gtgaactgta ggatatttat ataaaaataa gttattcgta agataattgg
     1441 ttgcttttaa ttgacaattc agtataatga ttacgaagta ggtgaaaaga catggaaaga
     1501 ttacaaaaag caattgcggc aagtggttat
//
LOCUS       DAOMTA010000813         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4355_length_1529_cov_2.422659, whole genome shotgun sequence.
ACCESSION   DAOMTA010000813 DAOMTA010000000
VERSION     DAOMTA010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_1529_cov_2.422659"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLK68_15925"
     CDS             complement(<1..902)
                     /locus_tag="PLK68_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM91939.1"
                     /translation="MEKLSKLSKDFLWGGAIAANQAEGAYVADGKGLSLMDIATSASK
                     DVSRQFTDGIKKGIYYPNHEGIDFYHKYKEDLALFEEMGFKCFRTSIAWSRIFPNGDE
                     KLPNELGLKYYDDLFDEMIKRGMEPVVTISHYEMPLYLAKQYGGWANRKLIDFYLNFC
                     EVIYKRYSGKVKYWMTFNEINSVIFMPEVAGIIGREQADFKQRSFQAAHHQFVASAKA
                     VKLGHQIDSKNMIGCMVLTLIKYPLTAKPEDVLLAEEKMRYGTFAFSDIQVRGHYPNY
                     VKKLIQKIDLKIEVKPDDLKSLKEG"
     gene            complement(886..>1529)
                     /locus_tag="PLK68_15930"
     CDS             complement(886..>1529)
                     /locus_tag="PLK68_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM91940.1"
                     /translation="IIKIGLSLPTIFLLYFVANLLWFFGIHPNTVYSAFVPLQMTLIM
                     TNIADAQAGKPLTYLTITLVSLFASFGGNGNTLGLCLSMFTARSERYKKMLKLAFIPN
                     LFNINEPLIFGMPVMLNPVFFIPMVFCNVVMGFIGLFATQIFTFTYNPAMSLLPWTTP
                     FFVKAFLAGGISLLIMVLILLVVNTLMYYPFFRIADKKAYEEEQLAKVGGKIE"
BASE COUNT          540 a          296 c          190 g          503 t
ORIGIN      
        1 ccttctttca aactttttaa atcatccggc tttacttcaa tttttaaatc tattttttgt
       61 attagttttt ttacataatt aggataatgt ccacgaactt gtatatcgct aaacgcaaat
      121 gttccataac gcattttttc ttcagctaat agcacatctt caggttttgc tgtaagagga
      181 tacttgatca aagttaatac catgcatcca atcatatttt ttgaatcaat ttgatgtccc
      241 aatttaacag ctttcgcact agcaacaaac tggtgatgtg cagcctgaaa acttcgttgt
      301 ttaaaatccg cttgttcacg gccaataatt cctgcgactt ccggcataaa aataactgaa
      361 ttaatttcat taaaagtcat ccaatattta actttaccac tatatcgttt ataaattact
      421 tcacaaaaat tcaaataaaa atcaattaac ttacgattag cccagccacc atattgctta
      481 gctaaatata acggcatttc ataatgagaa atagttacaa ccggctccat tcctcgttta
      541 atcatctcat caaataaatc atcataatat tttaaaccta attcattagg aagcttttca
      601 tcaccattag ggaaaatacg gctccaagcg attgaggtcc taaaacattt gaaacccatt
      661 tcttcaaata aagctaagtc ttctttatat ttatggtaaa aatctatccc ctcatgatta
      721 ggataataaa ttcctttttt aatcccatct gtaaactgtc gtgaaacatc tttgctggca
      781 ctagtcgcaa tatccattaa agataacccc ttaccatcgg caacatatgc tccttcagcc
      841 tgatttgcag caattgcacc tccccataaa aaatctttac ttaacttact caatttttcc
      901 accaactttc gctaattgct cttcttcgta ggctttctta tcagcaattc taaaaaatgg
      961 ataatacatt aaagtattta ctaccaataa aattagtacc atgatcaata atgaaattcc
     1021 tcctgctaaa aatgctttta caaaaaatgg agttgtccat ggtaataatg acatagccgg
     1081 attgtatgta aatgtaaaaa tttgggttgc aaacaaacca ataaagccca ttacaacatt
     1141 acagaaaacc attggaataa agaaaacagg attcaacatt actggcattc caaaaatcaa
     1201 tggttcattg atattaaaca aattaggaat aaaagctaat tttagcatct tcttatatct
     1261 ctcacttctc gctgtaaaca tcgataaaca taatccaagt gtattaccat taccgccaaa
     1321 agatgcaaac agtgaaacta aagtgattgt taaataagtt aatggttttc ctgcttgagc
     1381 atcagcaata tttgtcataa ttagtgtcat ttgtaatggt acaaatgcac tatataccgt
     1441 atttggatga attccaaaga accataataa attagcaaca aaatataata agaagattgt
     1501 cggtaatgat aatccaattt taataatga
//
LOCUS       DAOMTA010000814         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4356_length_1529_cov_2.298507, whole genome shotgun sequence.
ACCESSION   DAOMTA010000814 DAOMTA010000000
VERSION     DAOMTA010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4356_length_1529_cov_2.298507"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLK68_15935"
     CDS             complement(<1..190)
                     /locus_tag="PLK68_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91941.1"
                     /translation="MLKKRANSFDLSFRELKIYYSEKGYHLEDKLFETNLNLRNESGE
                     YNLLAKLLSDRNNILFIFC"
     gene            complement(491..616)
                     /locus_tag="PLK68_15940"
     CDS             complement(491..616)
                     /locus_tag="PLK68_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91942.1"
                     /translation="MKHTKDSLEKLEKIITIRIMQEDAETVYLFLIIMEEPFLLE"
     gene            complement(1009..>1529)
                     /locus_tag="PLK68_15945"
     CDS             complement(1009..>1529)
                     /locus_tag="PLK68_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91943.1"
                     /translation="YLITNKVYQLNKDIKKYFNCKDSKKAVQEYFNYFRNPILESYLK
                     TGEVYDTMRSLQKVCTNLNEDLLKLNKQIGKFINYDYLYEEELRDKIIITSGIDICPY
                     CGRQFITRFKDRSSADLDHFFPKKYLPLLSLSLYNFIPSCQICNSRFKRQHIKKFYIR
                     LMMDMKIMLYLM"
BASE COUNT          524 a          225 c          153 g          627 t
ORIGIN      
        1 cacaaaaaat aaatagaata ttatttctgt ccgataataa tttcgctagc aaattatatt
       61 cgccagattc atttcttaag ttcaaattcg tttcaaacaa tttatcttct aaatggtatc
      121 ctttttctga ataatatatc ttaagctctc taaatgataa atcaaatgaa tttgccctct
      181 ttttaagcat gtattcagta tcaataaatt tttttcatac cttattttta tttgatcaga
      241 gtcatctcct tacaggttgt ccctattctt atagtacaac cggtggatga aaagccatat
      301 ttcttttgac aataaatatg attgcgacca ttatttacat gaatagcaat aattgttttt
      361 ccatcatcaa ctttaagttc tgaactaatt tcatcttgtg gattaggtta tatctgactg
      421 gttataatat cgaatgtttt cctaagcact tcatctattt tatccacatc aattacaata
      481 ccattatctt ttactccaat aaaaatggtt cctccattat tattaagaaa agatacaccg
      541 tttctgcatc ctcctgcata atacgaatag taataatctt ttctaatttt tccaagctat
      601 cttttgtatg tttcattata gcacctctaa tttcctataa cctttatttt ccatctcaaa
      661 aattgattca atttcttcaa aaaagattat atatatcatt aacaagttta cctaaaggtt
      721 tattttgatc cttttctaaa tcaaaggaat agccaaaaag taatatattc atttgttcat
      781 tatcgagatt ataaggtacc aattttttta tattttcttt aaaagtagaa ttatcataaa
      841 tatgctttct atataataaa tctgcaacaa atacattatg actctggtat atttcatcaa
      901 tataaaataa tttaatatct tcagatgttt caaaatactt atcttttgaa atattttcta
      961 tctgaatttc tataacagga cgaattttag taatgggaga tttatatttt acattaaata
     1021 tagcattatt ttcatatcca tcatcaaacg gatatagaat ttttttatgt gttgtctttt
     1081 aaagcggcta ttacaaattt ggcaactagg aataaaatta tataaagata aggataataa
     1141 aggtaaatat ttttttggaa aaaagtgatc taaatcagct gaacttctat ccttaaatct
     1201 tgtaataaat tgtctaccac aatatggaca aatatcaatt cctgaagtaa taattatttt
     1261 gtctcttagt tcttcctcat acaaataatc ataatttata aattttccaa tttgtttatt
     1321 taatttgagt aaatcttcat taagattagt acatactttt tgtaaacttc tcattgtatc
     1381 ataaacttcg cctgttttta aatacgattc aagaattggg tttctaaaat aattaaaata
     1441 ttcttgaact gctttttttg aatctttaca attaaaatat ttttttatat ctttatttag
     1501 ttgatatact ttattagtta tcaaatatg
//
LOCUS       DAOMTA010000815         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4373_length_1525_cov_2.349660, whole genome shotgun sequence.
ACCESSION   DAOMTA010000815 DAOMTA010000000
VERSION     DAOMTA010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_1525_cov_2.349660"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PLK68_15950"
     CDS             <1..817
                     /locus_tag="PLK68_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM91944.1"
                     /translation="LLQTYFAAGEIDLDVSFYGLDDNSIYYADIDLPKEKDQIIISYD
                     FAKKVGLEKGDKVTFTNQYTEQEYQLKVFDIYNSRTNISVYMSRESLNNLLEEDLNYY
                     NSYLSDQKLNIDKKYLSTMITKTDMLKIGDQMTETFSQMIPIMSGIAIVIYLVVMYIL
                     TKLVLDRNTNYMSFLKVIGYNSQEIAKIYLKATALVVVFSLLISLPICKYGLEILFIQ
                     AMMRFAGYIEIYIPTYLYVMIFTVGLITYFVVNWLLNKQIQRIDLGKSLKETE"
     gene            859..>1525
                     /locus_tag="PLK68_15955"
     CDS             859..>1525
                     /locus_tag="PLK68_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM91945.1"
                     /translation="MLEDIKLPFIDKKVINKHEREYQIGLIDGQGVMTSIDIYPGIQV
                     IYNNFHCFTAPTDQSLNRHRYIEINHCLKGKFECQYNKNYYAYLCQGDLAISGGTLNK
                     LTHSFPLGYYNGVEILIEVEKAKENKLLDEFNIDIDLISKRLKESHNVYIFRATAQIE
                     HICLEMYEIEEELKRNYLKIKVLELLLFLSHHDFGILENEKHYYPKKQIEIIKAIKTE
                     LSNN"
BASE COUNT          582 a          159 c          253 g          531 t
ORIGIN      
        1 attattacaa acctattttg ctgctgggga aattgactta gatgtttcat tctatggatt
       61 agatgataat tctatatatt atgcggatat tgatttgcca aaggaaaaag atcaaattat
      121 tatttcctac gattttgcta aaaaagttgg cttagaaaaa ggagataaag taacgtttac
      181 aaaccaatat actgaacaag aatatcagtt aaaggttttt gatatttata atagccgaac
      241 taatataagt gtttatatgt cacgtgaatc attaaataat ttactcgaag aagatctgaa
      301 ttattataat agttatttat cagatcagaa gttaaatatt gataaaaaat atcttagtac
      361 gatgattact aagacagata tgctgaaaat tggtgatcaa atgacggaaa ccttttcaca
      421 gatgattccg attatgagtg ggatagcgat agttatttat ttagttgtaa tgtatattct
      481 aacaaaacta gtattagatc gaaatactaa ttatatgtcg tttttaaaag tgataggcta
      541 taacagccaa gaaattgcta agatatatct taaagctaca gctttggtag tagtattttc
      601 attattgatt agtttaccaa tttgtaaata tggtttagaa atattgttta ttcaagcaat
      661 gatgagattt gcaggatata tagaaatcta tattccaacc tacctctatg taatgatttt
      721 tactgttggc ttaattactt attttgttgt taattggcta ttgaataaac aaattcaaag
      781 aattgattta gggaaatctt taaaagaaac agaataacta taggttattc tgttttcacc
      841 taaaaggagg agattttaat gcttgaagat attaagctgc cgtttataga taaaaaggtt
      901 attaataagc atgaaagaga gtatcaaata ggtttaatcg atggacaggg agtaatgacg
      961 agcatagata tttatccagg gatacaagtt atatataata attttcattg ttttactgct
     1021 ccgactgatc aatctctcaa tcggcatcgt tatattgaaa ttaatcattg tctaaaaggt
     1081 aaatttgaat gtcagtataa taaaaattat tatgcatatt tatgtcaagg agatttagca
     1141 attagtggtg gaacattaaa taaattaact cactcatttc cattgggtta ttataatggt
     1201 gtagaaattt tgattgaagt agaaaaagct aaagagaata aattattaga tgaattcaat
     1261 attgatattg atttaatcag taaacgttta aaagaaagcc ataatgtata tatttttcgt
     1321 gctactgctc aaattgaaca tatttgttta gaaatgtatg agatagaaga ggaattaaag
     1381 aggaattacc taaaaataaa agtgttagaa ctgttattgt ttttatcaca tcatgacttt
     1441 ggaattttag aaaatgaaaa gcattattat ccaaaaaaac aaattgagat tatcaaggca
     1501 ataaaaacag agcttagtaa caata
//
LOCUS       DAOMTA010000816         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4379_length_1524_cov_2.219197, whole genome shotgun sequence.
ACCESSION   DAOMTA010000816 DAOMTA010000000
VERSION     DAOMTA010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4379_length_1524_cov_2.219197"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLK68_15960"
     CDS             <1..1098
                     /locus_tag="PLK68_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM91946.1"
                     /translation="SEFRTLMITLIRPLLGTLVAAFIQSFFLSFTDFGIPASVGGKYD
                     VVASVLYNKMLGSIPNFAGGAVVAMTMLIPSIISIILLHRLEKYNIRYNKISTIEIKQ
                     NRFRDWFWGTLSITINVIIIAVFIVIIIVPFVGEWPYRISFTFQNVINVFSDATLLGV
                     IKNSLITAIFTALLGTLVVYGAALASARSTLNKFLKKIIESIALATNTIPGMVLGIAY
                     LLMFSGTVLQNTYIIIILCNIVHFFSSPYLMMKNSLTKMNGSFETTAKLMGDSWFKTV
                     IRVITPNAISSILEVFSYYFINAMVTVSAVIFIAGARTMVMTTKIKELQHFAKFNEIF
                     VLSILILLINLLAKGVFSYFSKQRRERKTLK"
     gene            1095..>1524
                     /locus_tag="PLK68_15965"
     CDS             1095..>1524
                     /locus_tag="PLK68_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM91947.1"
                     /translation="MKKKLLKGLVVSSLLCTSLLTGCSSSNDQVVIYSNADDEAVTAM
                     KNALDKNGYEGQYLFQTFGTSELGGKLLAEGTNIEADLVTMSSFYLDSAQEQKKMFVD
                     LDFDTQALDEYPSYYTSITSQEGAIIINTEELKANSLETPT"
BASE COUNT          485 a          197 c          294 g          548 t
ORIGIN      
        1 agtgaattta ggacattaat gattactttg attcgacctt tattaggaac tttggttgca
       61 gcctttattc agtcattctt tctaagtttt acagattttg ggattcctgc atcggtgggt
      121 ggaaagtatg atgttgttgc tagtgtactt tacaataaga tgttgggaag tattccaaat
      181 tttgcaggtg gggcagttgt tgcaatgaca atgctgattc cttcaatcat cagtattatt
      241 ttattgcatc ggcttgaaaa atataatatt cgttataata aaatctctac gattgaaatc
      301 aaacagaatc gttttcgtga ttggttttgg gggacactga gcataacgat taatgtaatt
      361 attattgctg tctttatagt aattataatt gtgccgtttg ttggagagtg gccatatcga
      421 attagtttta cttttcaaaa tgttattaat gtttttagtg atgcaacatt gctaggggta
      481 ataaaaaatt cattaatcac tgcaatcttt acagcattac taggaacact tgtcgtatat
      541 ggagcagcgc tggctagtgc ccggagcact ttaaataaat ttttgaaaaa gataattgaa
      601 agtattgctc tagctactaa tactattcct ggtatggtat tagggatagc atacttgtta
      661 atgttctctg ggacagtgct ccaaaatact tatatcatca ttattttgtg taatatcgtt
      721 catttctttt catcgccata tttaatgatg aaaaactcat tgacaaagat gaatggttca
      781 tttgaaacaa ctgcgaaact gatgggtgat agctggttta aaacggttat tagagttatt
      841 acacctaatg caatttcatc aattttagaa gtatttagtt attattttat taatgctatg
      901 gtcactgtta gtgcggttat ctttattgct ggggcgcgga cgatggtgat gacgacaaag
      961 atcaaagagt tacagcattt tgctaaattt aatgaaatct ttgttttatc aatcttgatt
     1021 ttattaatta atttattagc taaaggagta tttagttact ttagtaagca aagaagagaa
     1081 aggaaaacat taaaatgaaa aagaaattat taaaggggtt agtagttagt tcgttacttt
     1141 gtacttcact tttaactgga tgttcttcat caaatgatca ggttgttatt tattcaaatg
     1201 ccgatgatga agctgtaacg gcaatgaaaa atgcattaga taaaaatggt tatgagggac
     1261 agtatttatt tcagactttt ggaacttctg aattaggagg taagttattg gctgaaggaa
     1321 ctaatattga ggcagatcta gtaacaatga gctcgtttta tcttgatagt gctcaagaac
     1381 aaaaaaagat gtttgttgat ttagattttg atactcaagc attagatgaa tatccatctt
     1441 attacacatc gattacttca caagaagggg ctattattat taatactgaa gaattaaaag
     1501 caaatagtct tgaaacacca acct
//
LOCUS       DAOMTA010000817         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4381_length_1524_cov_1.943499, whole genome shotgun sequence.
ACCESSION   DAOMTA010000817 DAOMTA010000000
VERSION     DAOMTA010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_1524_cov_1.943499"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..765)
                     /locus_tag="PLK68_15970"
     CDS             complement(160..765)
                     /locus_tag="PLK68_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91948.1"
                     /translation="MKSIKIIIALLMIFVCIPVSNISALESTDNSNIIIYTPTEEEFR
                     AAEEIEIAKMKAYFERTKESYAYDYRKVGDPQTSSFSPYKNAYDQPAGGTIFNSENSG
                     FYWSDSSRSPGTWSLGISIAGKYLSVDVAYSPGVVSGSSGGVFVGISSTQVGKAVKLK
                     VARNYKVQRYDVYRKPQYGGSWTYLSSYGAPTKYQSRFIIE"
     gene            939..1328
                     /locus_tag="PLK68_15975"
     CDS             939..1328
                     /locus_tag="PLK68_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91949.1"
                     /translation="MKNKYKIIVIVVIVFLLSSFYFGYSLGIDADKPNVINKISGTYQ
                     LFKGNETLTVTIDDQDNLYYQNGFENESIKIKTQINKLYENLYLFENERLSYNLILFN
                     DNFLLLININDQTYAKLEKVSNTQVFL"
BASE COUNT          564 a          194 c          187 g          579 t
ORIGIN      
        1 gtacaacttt ttacttataa atacgtataa ataataccca cttattatac ctttttttga
       61 ttatgcaatt gttagaaact gtttttatta acaagttatt attttgataa tgctatataa
      121 ataatatatt ttgacaatgt tataaatgat aacattgttt tattctataa taaatcgact
      181 ctgatacttt gtaggtgcac catatgaaga gagatacgtc catgatccgc catattgtgg
      241 ttttctgtat acgtcatatc tttgaacttt ataatttctt gctactttta attttacagc
      301 tttacctact tgagttgatg atatgcctac aaatactcca ccactagaac ctgataccac
      361 accaggagaa tatgctacgt caacagataa atattttcca gcaatactaa tccctaaaga
      421 ccatgttcct ggacttcttg aactatcact ccaatagaaa ccgctatttt ccgaattaaa
      481 aatagtacct cctgctggtt gatcataagc atttttatat ggtgaaaatg aagatgtttg
      541 agggtcacca acttttctat aatcataagc atatgattct tttgtccgct caaaataagc
      601 tttcattttt gcaatttcta tttcttcagc tgctctaaat tcttcttctg ttggggtata
      661 tataataata ttgctattat cagtactttc taatgcagaa atattggaaa caggaataca
      721 aacaaaaatc attagtagag cgattataat tttaatagat ttcataactt tttcatctcg
      781 catataattt aaatatttat tatagcatta tcttatataa atattgtaat ataaactagc
      841 acatttttct atataaagtc aaaaaaatat gattataagg ttattatttg atttatcgtt
      901 atataacgta gtataatttt tttgtaggag gaatagatat gaaaaataaa tacaaaataa
      961 ttgttatagt ggtaatagta tttcttcttt cgtcttttta tttcggatac tcattaggaa
     1021 tagatgcaga taaacctaat gtaattaata aaatatcagg aacatatcaa ttatttaaag
     1081 gtaatgaaac attaacagta acaattgatg atcaagataa tctttattac caaaatggat
     1141 ttgaaaatga aagtattaaa ataaaaacac aaataaataa attatacgaa aatttatatt
     1201 tatttgaaaa tgagaggcta agttataatt taatcttatt taatgataat tttttattgc
     1261 ttataaatat taatgatcaa acatacgcca aattagaaaa agtttcgaac acacaagttt
     1321 ttttataaaa aagaagaggt ataccctata attttgtaat ttaaatatat atattttctc
     1381 attattaata atgttgattt attaaagtat taaaagtcta atatggctgt ttactgcttt
     1441 aaggatatga atatggggga tattagtaga ttttactaaa cattacaatt atattaatta
     1501 aatatgaaaa ccttaatttg tcag
//
LOCUS       DAOMTA010000818         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4395_length_1520_cov_1.501706, whole genome shotgun sequence.
ACCESSION   DAOMTA010000818 DAOMTA010000000
VERSION     DAOMTA010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4395_length_1520_cov_1.501706"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLK68_15980"
     CDS             complement(<1..371)
                     /locus_tag="PLK68_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM91950.1"
                     /translation="MTSFFTATSATCVTGLIRVDTYSHWSWFGQFIIILLIQIGGIGF
                     MTFCIYALTLAKKKIGIISRSIMQNSISSPHVGGIVKMTKFIILATFFIEALGAFFLS
                     FIFCPKLGLVKGLWFSIFHSIS"
     gene            716..850
                     /locus_tag="PLK68_15985"
     CDS             716..850
                     /locus_tag="PLK68_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91951.1"
                     /translation="METVGLLILMIIILGGGYLLTGSLDTLLDHLTIKDNHSNKPLSK
                     "
     gene            complement(1015..>1216)
                     /locus_tag="PLK68_15990"
     CDS             complement(1015..>1216)
                     /locus_tag="PLK68_15990"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM91952.1"
                     /translation="VESIKTVTDTPVAVGFGINSPAQVKHFGNIADGVIVGSAIVNLI
                     KEHGDRAHQPLFNYIQTLIKEL"
     assembly_gap    1217..1226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1227..>1520)
                     /locus_tag="PLK68_15995"
     CDS             complement(<1227..>1520)
                     /locus_tag="PLK68_15995"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM91953.1"
                     /translation="HYGYDEFFKKCQETGVDGIIIPDCPYEERKEVSDICYKYDVDLI
                     SMIAPTSKERTLEIAAQAKGFIYLVSSMGVTGMRSNIVTDIEGTTRDVLEEQIR"
BASE COUNT          537 a          270 c          185 g          518 t
ORIGIN      
        1 gaaatactat gaaaaattga gaaccacaaa cctttaacca atcctaattt tggacaaaaa
       61 ataaatgaaa gaaaaaaagc ccctaacgct tcaataaaaa aagtcgccaa gataataaat
      121 tttgtcattt ttacaattcc cccaacatgt ggagaagaaa ttgaattctg catgattgaa
      181 cgactaataa ttcctatctt ctttttagct aatgttaaag cataaataca aaaagtcata
      241 aaaccaatac caccaatttg aatcaataaa ataattataa actggccaaa ccagctccaa
      301 tgactatatg tatctacccg aatcaagcct gtcacacaag ttgctgaagt ggcagtaaaa
      361 aaagaagtca ttattgaaac ttcctggttt tctttcaacg agattgggag actcaagaga
      421 atcgtcccca ttaaaataat aatacaaaaa ccatagaaaa taatcttcat tggcgtaaga
      481 ccatgcaata atttctcagt tagtttttct ttgataggac atcgctccct ttacctatac
      541 tttatcttat aagttcacta tactaaacta ttcataaata taaaataaag atttcctcaa
      601 tagtattaag attgtataaa gatagaaatt atctttatac aatcttaatc ttattagtga
      661 atatcttacg tcatttttat actatttttg gtattttatc tatagaggtg gtagtatgga
      721 aacagttgga ctattaattt taatgattat tattttaggc gggggctatt tattaaccgg
      781 ttcgttagac accttattag atcatttgac tattaaagac aatcattcaa ataaaccctt
      841 atcaaaataa tactttttta agttaattta aactaaattt aacagcaatc cgatagattg
      901 ctgtttttta gttttatttt atactataaa tcaaattcat ttttttaaac gaactattat
      961 taacagtcta aactaattaa aatttcaata atttaatccc ttctaattat ccttttataa
     1021 ttccttaatc agtgtctgga tgtaattaaa taatggttga tgcgcacgat caccatgttc
     1081 tttaattaaa ttaacaattg cactgccaac aattacacca tcagcaatat ttccaaaatg
     1141 cttcacttgt gctggactat taatgccgaa tcctaccgct actggtgtat cagtaaccgt
     1201 tttaattgat tcaacannnn nnnnnnacgg atctgctcct ctaagacatc tcttgtcgtt
     1261 ccttcaatat ctgtaacaat attgcttcgc atacctgtaa ctcccattga agaaactaaa
     1321 taaataaaac cttttgcctg ggctgctatt tcaagtgttc gctcttttga tgttggagca
     1381 atcatagaga ttaaatcaac atcatattta taacaaatat cactaacttc ctttctttct
     1441 tcatacgggc aatcaggaat gatgatccca tcaactccgg tttcttgaca ttttttaaaa
     1501 aattcatcat atccatagtg
//
LOCUS       DAOMTA010000819         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4400_length_1519_cov_1.774590, whole genome shotgun sequence.
ACCESSION   DAOMTA010000819 DAOMTA010000000
VERSION     DAOMTA010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_1519_cov_1.774590"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>1519
                     /locus_tag="PLK68_16000"
     CDS             104..>1519
                     /locus_tag="PLK68_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM91954.1"
                     /translation="MDNLEQTPRITLLKEKMLNEPRYVSIEQARIITRIYQENESLSI
                     PKKRALSLKAALEELEIGVEKEELIVGNRTKGVRYGVVFPESGCSWVNKEFETLPTRP
                     QDKFRIKKEDVKEFKEIIYPYWQDRSLEDVIKENYGEEINAIAKVVKINQKDHAQGHI
                     CPDTKTWLELGPKGLMTKAYEKLKNCDENQKEFYECTIIVLEGVCHFMMRYHDYILTM
                     LESLEDDNKKSLQRVADICANLASRPAQSFHEAVQSLWFLFVVLHMESNASSFSPGRM
                     DQYLYPYYQKDIEKGIISKQEALEILECLWLKFNQIVYLRNQHSAKYFAGFPIGFNIA
                     IGGIDENGCDIYNELSLLLLKAQYHLGLPQPNLSVRLNKNSSHELIQEAIKVVAKGSG
                     MPQFFNDEAIVNSMIKDLGIEEKDARNYAIVGCVELTTHGNNLGWSDAAMFNLNKALE
                     LTMNHGKCLLTNEPIGLDLGSI"
BASE COUNT          578 a          170 c          296 g          475 t
ORIGIN      
        1 ttattatttt ttattgaaaa tatattgaaa aatattgtat atgtattata attcaattat
       61 aaacaataaa aaacgataaa aaatgagatg aggagaatta gctatggata atttagaaca
      121 aactccaaga attactttat taaaggaaaa aatgttaaat gaaccacgtt atgtttcaat
      181 tgaacaggca cgaattataa cgaggattta tcaagaaaat gaaagcttat caattcctaa
      241 aaaaagagcg ttatctttga aagcagcatt agaagaatta gaaattggcg tagaaaaaga
      301 agagttgatt gttgggaatc gcactaaagg tgttaggtac ggagtagtat ttccagaaag
      361 tggatgttct tgggttaaca aagagtttga gactttacca acaagaccac aagataaatt
      421 ccgaataaaa aaggaagatg taaaagaatt taaggagata atttatccat attggcaaga
      481 ccgttcattg gaagatgtca taaaagagaa ttatggtgag gaaattaatg caattgctaa
      541 agttgttaaa attaatcaaa aagatcatgc tcaaggacac atttgtccag atacgaagac
      601 ttggctagag cttggaccaa aaggattgat gacaaaagct tatgaaaagt taaaaaattg
      661 tgatgaaaat caaaaggaat tttatgaatg tacaattatt gttttagaag gtgtctgcca
      721 ttttatgatg cgttatcatg attatatttt gacaatgttg gaaagtctgg aagatgataa
      781 taagaaatct ttacaaaggg ttgcagatat ttgtgcaaat cttgctagtc gacctgctca
      841 gtcatttcat gaggctgtac aatcactttg gtttttattt gttgttttac acatggagtc
      901 taatgcatca tcattttctc ctggaagaat ggatcaatat ctttatcctt attatcaaaa
      961 agatattgaa aaaggaatta tatcaaagca agaggcatta gaaatattag agtgtttgtg
     1021 gttaaagttt aatcaaattg tgtatttgag gaatcagcac agtgcaaaat attttgctgg
     1081 ttttccaatc ggatttaata tagcgattgg tggaattgat gaaaatggat gcgatattta
     1141 taatgagtta tcactactgt tattaaaagc acaataccat ctaggattac cacaacctaa
     1201 tttatcagta agattaaata aaaattcttc acatgaacta atacaggaag caatcaaagt
     1261 agttgctaaa ggtagtggga tgccacagtt ctttaatgat gaggcaattg taaatagtat
     1321 gattaaagat ttaggtattg aagaaaaaga tgcaagaaat tatgcaattg ttggatgtgt
     1381 tgaattaacg acacatggaa acaatttggg ctggtctgat gctgcaatgt tcaatttaaa
     1441 taaagcgctg gaattaacta tgaatcatgg aaaatgtctt ttaactaatg aaccaatagg
     1501 tttggatctt ggaagtatt
//
LOCUS       DAOMTA010000820         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4409_length_1517_cov_2.041040, whole genome shotgun sequence.
ACCESSION   DAOMTA010000820 DAOMTA010000000
VERSION     DAOMTA010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4409_length_1517_cov_2.041040"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1084)
                     /gene="xerC"
                     /locus_tag="PLK68_16005"
     CDS             complement(176..1084)
                     /gene="xerC"
                     /locus_tag="PLK68_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRM91955.1"
                     /translation="MKTTLDNYFRQYLDYLQYQRHYADKTIESYKRQIDHFKQFLIEE
                     SIDDYNDVSYAMLRGYLTKLYEKNLSKTTINHKLSALRSFFNYLLKEELINDNPFLLI
                     ESQKVAKRNPDFLFPEEILGLLDSIETKDDLGIRNKAMMELMYASGLRCSEVVNLQLS
                     NIDFNQMVLFIHGKGNKDRYVPFHDYAGEWLIKYIQEARENLMIKNEGHNFVFVNKFG
                     NPLTNRGVENIVDRIMRLYDSTKKIHPHTIRHSFATHLLNAGADIRTVQELLGHENLS
                     TTQIYTHISRDHLKEVYLKAHPRNIE"
     gene            complement(1065..>1517)
                     /locus_tag="PLK68_16010"
     CDS             complement(1065..>1517)
                     /locus_tag="PLK68_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM91956.1"
                     /translation="EQKRIIQMIPGLENASFVRYGVMHRNSFICSPKHLLKTYQLKNY
                     SNIFMAGQITGVEGYVESAQSGMAAGINMVRLLEDKEPLIFPENTVMGALANYITNAS
                     KEDFQPMKANFGILPDFPIRIKKKERKAAYASRALETMKGFVDENNLG"
BASE COUNT          475 a          266 c          201 g          575 t
ORIGIN      
        1 tgaaattact gacattgtaa cttcctcctt ttgttttttc ctccattatt tcgaacattt
       61 atcaccttac ggcactagat aaatgaattc ataatgtgtg tatttttcat acctgaaaca
      121 ttttatcata gatttttcaa tcatacaagc atatttttca tcattttaac ttgaattatt
      181 caatatttcg tggatgtgct tttaaatata cctcttttaa atgatctctt gaaatatgtg
      241 tataaatttg agtggttgat aagttttcat gacctaacaa ctcctgaaca gttctaatat
      301 ctgctcctgc atttaataaa tgtgtagcaa aggaatgtcg aattgtatga ggatgaattt
      361 ttttggttga atcataaagt cgcattattc gatctactat gttttctact cctcggttag
      421 ttagaggatt accgaattta ttaacaaaga cgaaattatg cccctcattt tttatcatta
      481 aattttcacg agcttcttgt atgtatttaa tcaaccattc tccagcataa tcatgaaatg
      541 gaacataacg atctttattc ccttttccat gaataaataa taccatttga ttaaaatcta
      601 tatttgaaag ttgtaaattt acaacctctg aacaccgcaa gcctgatgca tacattaatt
      661 ccatcatcgc tttattacga attcccagat catcttttgt ttcaatacta tctaatagtc
      721 ctaggatttc ttcaggaaaa agaaaatccg gattacgctt tgcaactttt tgggattcaa
      781 tcagcaaaaa aggattatca tttattaact cttcttttaa taaatagtta aaaaaacttc
      841 ttaacgcact gagtttatga ttaattgttg ttttagaaag atttttttca tatagcttag
      901 taagatatcc acgtagcatt gcataactca catcattata atcatcaata gattcttcaa
      961 taagaaattg tttgaaatga tcgatctgac gcttatacga ttctattgtt ttatcagcat
     1021 agtgacgctg atattgtaaa taatcaagat attgtcggaa atagttatcc aaggttgttt
     1081 tcatcaacaa atcccttcat tgtttccaac gctcttgatg cataagcagc tttacgttct
     1141 tttttcttga ttcgtattgg aaaatctggc aaaattccaa aattagcttt cattggttga
     1201 aaatcttctt tcgaggcatt agttatataa ttagccaacg ctcccataac agtattctcc
     1261 ggaaaaatca aaggctcttt atcttctaat aaccggacca tgtttattcc ggcagccata
     1321 ccactttgag cagattctac atatccttct actccagtaa tttgacctgc cataaatata
     1381 ttagaataat tttttaactg gtatgtcttt aatagatgtt ttggtgaaca aataaaacta
     1441 tttcgatgca ttactccata tcgaacaaaa cttgcatttt caagtcctgg aatcatttga
     1501 ataattcttt tttgttc
//
LOCUS       DAOMTA010000821         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4416_length_1516_cov_1.995209, whole genome shotgun sequence.
ACCESSION   DAOMTA010000821 DAOMTA010000000
VERSION     DAOMTA010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_1516_cov_1.995209"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLK68_16015"
     CDS             <1..658
                     /locus_tag="PLK68_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRM91957.1"
                     /translation="ISTLIGVGSSICGGSAIAATAPVIEADDEEIAQAISVIFLFNIL
                     AALIFPTLGGMLGLSNDGFGLFAGSAVNDTSSVTATATAWDGIHGSNTLDQATIVKLT
                     RTLAIIPITLVLAFKRTRDAEKSESTFNLKKIFPFFILFFILASVITTVFNLPGNITA
                     PIKDLSKFFIVMAMAAIGLNTNIVKLVKSGAKPIFMGFCCWVAITVVCLSMQALLGLF
                     "
     gene            complement(710..1462)
                     /locus_tag="PLK68_16020"
     CDS             complement(710..1462)
                     /locus_tag="PLK68_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRM91958.1"
                     /translation="MDIKSLLELFRKSPELFLDTSGRSGAKTYCYQDYYLKIGKKHEL
                     EAEYRALIYFSKYNYSQAALEYSSSERDYLLTKRMSGMCSCAPKLISNPEILAVTLGK
                     LLRKFHDTKFTEAISINHNEEILKRVFLNYHQANLDEQMTQGIGCLELDEVFSYINAK
                     KDILVEDTVIHGDYCLPNIFLDQDYRFCGFLDMGRAGVGDRHYDLFWGRWSLAYNLGN
                     DKYGDLFYEAYGNEVIDPERLKLIAYIACLDG"
BASE COUNT          473 a          294 c          216 g          533 t
ORIGIN      
        1 gatttcaaca ctgatcgggg ttggttcatc aatttgtggt ggttctgcaa tcgctgcaac
       61 cgcaccagtt atcgaagctg acgatgaaga aatcgctcaa gctatatcag taatcttttt
      121 attcaatatt cttgccgcct taattttccc aactttgggt ggtatgctag gattatcaaa
      181 tgatggtttt ggactttttg ctggaagtgc agtcaatgat acatcctctg ttaccgccac
      241 tgcaactgca tgggatggta ttcatggaag taatacccta gatcaagcaa cgattgttaa
      301 attaacaaga acactagcaa ttattccaat taccttagta ttagctttta aacgtactcg
      361 cgatgctgaa aaaagtgaaa gtacttttaa tctcaagaaa atttttcctt tctttatctt
      421 attctttatc ctagcttctg ttattacaac agtcttcaac ctgcctggta atattaccgc
      481 accaattaaa gatcttagca aattttttat tgttatggca atggcagcta tcggattaaa
      541 tacaaatatt gttaaacttg taaaaagcgg tgccaaacca attttcatgg gattttgctg
      601 ttgggtggct attactgtgg tttgtttatc aatgcaagca ctattaggtt tattttaaaa
      661 tgacgaatat tcgtcatttt ttatttttaa aacgccaagt tatttacctt tatccatcta
      721 gacaagcaat atatgcgatt aatttcaaac gctctggatc aattacttca tttccatatg
      781 cttcataaaa taaatcacca tatttatcat tacctagatt ataagcaagt gaccatcttc
      841 cccaaaacaa atcataatgg cgatcaccta ctcccgcacg tcccatatcc aaaaaaccgc
      901 aaaaacgata atcttggtct aaaaaaatat ttggcaaaca ataatcacca tgaataactg
      961 tatcttcaac taaaatatcc tttttagcat taatataact aaacacctcg tcaagttcta
     1021 agcagccaat accttgtgtc atttgctcat ctaagttggc ttggtgataa tttaaaaata
     1081 cacgttttaa tatttcttca ttatgattta tagatatagc ttcagtaaat tttgtatcat
     1141 ggaactttct taacagcttt cccaaagtta ctgctaatat ctctggatta gaaattagtt
     1201 ttggggcaca gctgcacata cctgacattc tcttagttaa taaataatct cgctcactac
     1261 tgctatattc tagcgccgct tgactgtaat tatacttact aaaatatatt aatgcccgat
     1321 actctgcctc taattcatgt ttttttccaa tttttaaata ataatcttga tagcagtagg
     1381 ttttggcacc agatctccca cttgtatcta aaaatagttc gggagatttt ctaaataact
     1441 ctagcaaact cttaatatcc atataaagat cacctgcctc ttttaaaaat catctttttt
     1501 tattattata tcaagg
//
LOCUS       DAOMTA010000822         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4422_length_1515_cov_2.296575, whole genome shotgun sequence.
ACCESSION   DAOMTA010000822 DAOMTA010000000
VERSION     DAOMTA010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4422_length_1515_cov_2.296575"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLK68_16025"
     CDS             <1..766
                     /locus_tag="PLK68_16025"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538995.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRM91959.1"
                     /translation="MYLEKLILNPKHIEFQILADNYGNVIHLGERDCSIQRRNQKMIE
                     EAPSKALTPLLRQKMGEDAVKAAKGAGYRNAGTIEYVLDQDGNYYFIEMNTRIQVEHP
                     ITEMVTGVDLIREQIRIAANQKLTYKQSDIHLNGHAIECRINAENPREGFRPCPGTVN
                     SVHLPGGLGVRIDTTLYQGYKVSSHYDSMIAKVIVHGSNRLEAIRRMRRVLAELVIDG
                     IDTNQELQYLILHTGEYVKGNFDTSFIENNLDKLVG"
     gene            770..>1515
                     /locus_tag="PLK68_16030"
     CDS             770..>1515
                     /locus_tag="PLK68_16030"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HRM91960.1"
                     /translation="MEELFKARKEKLNLFKSIRNKMQDKKRIDVPDGLYTKCDSCGES
                     ILSEDLKESYYVCPKCGAHLKMRAYTRLNLLYDGGKYKELYKSIKSNDPLMFPGYKEK
                     LVKLEETTNLDEAVVCATGRIDGRKVVVCVMDSRFLMGSMSGAVGEKITRAIEHATKR
                     KCPIIIFTTSGGARMQEGIISLMQMAKTSAALAKHHEAGLLYISYITHPTTGGVTASF
                     AMLGDIIIGEPKALIGFAGPRVIESTIKQK"
BASE COUNT          543 a          188 c          320 g          464 t
ORIGIN      
        1 tatgtattta gaaaaattaa ttttaaatcc taaacatatt gaatttcaaa ttttagccga
       61 taattatgga aatgttattc atttaggaga acgcgattgt tctattcaac gacgcaatca
      121 gaagatgatt gaagaggcac caagtaaagc cttaacaccg ctgctccgac aaaaaatggg
      181 ggaagatgca gttaaagcgg ctaaaggagc aggttatcgc aatgcaggaa cgattgaata
      241 tgttttagat caagatggaa attattattt tattgaaatg aatactcgaa ttcaagttga
      301 gcatccaatt actgaaatgg tgaccggggt agatttaatt agggaacaga ttagaattgc
      361 tgcaaatcaa aaattaactt ataaacaaag tgatattcat ttaaatggac atgcgattga
      421 atgccgaatc aatgctgaaa atccacgtga gggattcaga ccgtgtccgg gaactgttaa
      481 tagtgttcat ttaccaggag gtttaggggt gagaattgat acaactttat atcaggggta
      541 taaagtttca tcacattatg attccatgat tgctaaagtt attgttcatg gttctaatcg
      601 cttagaagca attagaagaa tgcgtagagt tttagctgaa ttagttattg acggaattga
      661 tactaaccaa gaattacaat atttgatttt acatacggga gagtatgtaa aaggaaattt
      721 tgatacaagt tttattgaaa ataacttaga taagttggtg ggataataaa tggaagagtt
      781 atttaaggca cgaaaagaaa aattgaattt atttaaatct attcgtaata aaatgcaaga
      841 taaaaaaaga attgatgtac ccgatggttt atatacaaag tgtgattctt gcggagagtc
      901 gattttaagt gaagatttga aagaaagtta ttatgtttgt cctaaatgtg gagcacattt
      961 aaagatgcgg gcatacacgc gattaaatct tttgtatgat gggggaaaat ataaagaatt
     1021 atataaaagt attaaatcaa atgatccatt aatgtttcct ggttataaag aaaaattagt
     1081 aaaattagaa gaaacaacaa atcttgacga agcggttgtt tgtgctacag gaagaattga
     1141 tggaagaaaa gttgttgttt gtgtgatgga ttctcgtttt ttaatgggga gtatgtcagg
     1201 tgctgtcgga gaaaagatta ctagagcaat tgaacatgcg acaaaacgca aatgtccaat
     1261 cattattttt acaacttctg ggggagctag aatgcaagag ggaattattt cactaatgca
     1321 aatggctaaa actagtgctg ctctagccaa acatcatgaa gccggattgt tatatatttc
     1381 gtatattact catcctacta ctggtggagt tactgcttct tttgccatgc ttggggacat
     1441 tattattggg gaacctaaag cattaatcgg atttgcggga ccaagagtaa ttgaatcaac
     1501 aattaaacaa aaatt
//
LOCUS       DAOMTA010000823         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4433_length_1512_cov_2.155800, whole genome shotgun sequence.
ACCESSION   DAOMTA010000823 DAOMTA010000000
VERSION     DAOMTA010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4433_length_1512_cov_2.155800"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLK68_16035"
     CDS             complement(<1..221)
                     /locus_tag="PLK68_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM91961.1"
                     /translation="MSDSEKTKKIFAKNLLKYMERHNLNQTDISEITGVSQQSVSNWL
                     NAKLMPRMGIIEILAEYFKILKSDLLEEK"
     gene            354..638
                     /locus_tag="PLK68_16040"
     CDS             354..638
                     /locus_tag="PLK68_16040"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM91962.1"
                     /translation="MTKDINIPKISLKAARVNAELNQQEAAELLEIDVSTLIRWEKDP
                     RIVKSGYHEIISQVYHYPTDYIFLVTIQVKLVKTNQGESFLNVIKIRKDN"
     gene            910..1164
                     /locus_tag="PLK68_16045"
     CDS             910..1164
                     /locus_tag="PLK68_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91963.1"
                     /translation="MAVRADTKTTIVDFSDKTEEMEDTTIVYFCIEGDNFLWGYEPFE
                     NADFSIVELSNVKELQKLIEEKRLSVDDLTLILKTIKLPF"
     gene            complement(1148..1384)
                     /locus_tag="PLK68_16050"
     CDS             complement(1148..1384)
                     /locus_tag="PLK68_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91964.1"
                     /translation="MQIEHDHVSMESELEFHWTEIMSNLNDFNALLGTDFTPLSSRNL
                     ITDKTINAITKSLIEDDGKIEISKLAPYITRKAI"
BASE COUNT          531 a          185 c          256 g          540 t
ORIGIN      
        1 tgtttttcct ctaataaatc cgatttaagt attttaaagt attctgctaa tatctcaatt
       61 atccccattc taggcatcaa tttagcattt aaccaattag atactgattg ttgtgaaaca
      121 cctgtgattt cagaaatatc agtttgattt aagttgtggc gttccatata ttttaaaaga
      181 ttcttagcaa atatcttttt tgttttttca gaatcactca ttttaattac ctcctgataa
      241 taatatttta tagtaaaact tgtattaatg caataaaata taacattttt acaattttta
      301 cttgatatac aagtttaact tgtgtataat taagttcgta agggggtggt catgtgacaa
      361 aagatattaa tattcctaaa atatcattaa aggcagcaag agtaaacgct gaattaaatc
      421 agcaagaggc agcagaatta ctcgaaattg atgtttctac gctgattagg tgggaaaaag
      481 acccgcgaat tgtaaaaagt gggtatcacg aaattatttc gcaagtttat cactatccaa
      541 ctgattatat ttttttggtc acaatacaag ttaaacttgt taaaactaat caaggagaaa
      601 gtttcttaaa cgtaataaag ataagaaagg acaattagaa tgaaaatatt tttatgtatg
      661 ctttttgtaa ttggatttgc attatcaagc ggagtaggtg tgtttttgct attaggcata
      721 gctattatgg aagactataa atcattgata aaaccaactt tgtttgcttt tgggttaagt
      781 gcagtattag tgattatatc ttttatctta atactgtcga ctaactaaat cataacagtt
      841 ttcaaaatta tgtgatttga taaatcgttg ttgaaagtgg agttaaaaag aagttaggag
      901 gagtaaagga tggcagtaag agctgataca aaaaccacga ttgtagattt cagtgacaaa
      961 actgaggaaa tggaagatac aacaatagtt tatttttgca tcgaaggtga taattttttg
     1021 tggggttatg agccgtttga aaatgctgat ttcagtattg tcgaattatc aaatgttaaa
     1081 gaattacaaa aattgattga agaaaaaaga ttaagcgttg atgacttaac cttaattcta
     1141 aagactatta aattgccttt ctagtaatgt atggagctaa tttagatatt tcgattttac
     1201 cgtcatcttc aataagtgat ttagtgatag cgttaattgt tttgtcggtt attaaattac
     1261 gagaagaaag tggtgtaaaa tcagttccta acagtgcatt aaaatcattt aagttggaca
     1321 tgatttcagt ccaatgaaat tctaattcgc tttccatact aacatggtcg tgttcaatct
     1381 gcatataata tctcctttca ttagatttca acgtggcagt gttgatgcat atattatatc
     1441 aaatttaata attataatct aaaaaaggga gttaaacaaa aagctaacta tatataaatc
     1501 aagtgatttt tg
//
LOCUS       DAOMTA010000824         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4439_length_1511_cov_1.951236, whole genome shotgun sequence.
ACCESSION   DAOMTA010000824 DAOMTA010000000
VERSION     DAOMTA010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_1511_cov_1.951236"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..1104)
                     /locus_tag="PLK68_16055"
     CDS             complement(454..1104)
                     /locus_tag="PLK68_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM91965.1"
                     /translation="MIRYCDNAGTLIPPLFFLPLLEGVELVDLIDFFVLDTVCQKIKY
                     WLDKEMKIIPLSINFSIESLRGKSFVERILETCKKYQIPTKYIEIEITERVHDEKNFE
                     IKTVISKLRSAGFIVAIDDFGTEYANLALLSDAEFDILKLDKSLISNVALNPRTKIIM
                     EYISKICHRLGVDMIAEGIESEEQFFTLCSYGVETVQGYLFSKPLAINVFEEKYLS"
     gene            complement(1101..>1511)
                     /locus_tag="PLK68_16060"
     CDS             complement(1101..>1511)
                     /locus_tag="PLK68_16060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM91966.1"
                     /translation="KMVFKKADFYRIGGDEFIIIIICQSIKKESFEKRVKELSESFSK
                     KPVCQVAIGTQWTNAVGNINEMIAEADARMYENKKEFYHKHMISRRYRHHSDEMLHLT
                     NIDYLESEIENGHFVVYLQPKILCEDRSVLGQKH"
BASE COUNT          524 a          287 c          159 g          541 t
ORIGIN      
        1 cattgctaaa gccataatta gcgaaatgat tctccttttc cctaaccttt tcattttttt
       61 gctcctttac ccttctctag tctaaaataa atcaaaggcc taaataaatt gcaacaagta
      121 tttgatactt gcggattatt accaagtact aacaatattt gttttccctt aatttttcac
      181 actccttgtc catctatttt gtcatacttg attgaatcag taaacattca ttacttttaa
      241 aagaataaaa cctaagtaaa gcaagcaaaa aaattgctca tactaatact taggctataa
      301 ttgatttaaa ggtaatacca cgtctatata cttatattct agataattat atagacaatg
      361 tcaatgacaa ctaaaaaata aagtcaaata aatgttatta tttatacact tcacatacaa
      421 caccttaaat aaactttaat tagcttctta aaattaactt agatactttt cttcaaaaac
      481 atttattgcc aacggcttgc tgaataaata accttgaact gtttcaaccc catatgaaca
      541 cagcgtaaaa aactgctctt cagattcaat tccttccgct atcatatcca cccctaatcg
      601 atgacaaatt tttgaaatat attccatgat tatcttagtt cttggattta atgcaacatt
      661 actaattaag ctcttatcca attttagaat atcaaactca gcatccgata ataatgctaa
      721 attggcatac tccgttccaa aatcatcaat agctacaata aatccagcac tacgaagttt
      781 tgatattacc gttttaattt caaaattctt ttcgtcatgt actcgttctg ttatttctat
      841 ttctatatat ttagtaggta tttgatattt tttacaagtt tctaaaatac gttcaacaaa
      901 agatttacca cgaagtgatt ctatagaaaa atttattgaa agcggaatta tcttcatttc
      961 tttatctaac caatatttta ttttttgaca aactgtatca agaacgaaaa aatcaataag
     1021 atcaactaat tctacccctt ctaataatgg taaaaagaaa agtggcggaa ttaatgtccc
     1081 agcattatca cagtaacgta tcaatgcttc tgccccaaca ctgagcgatc ttcacataaa
     1141 attttaggtt gtagatagac aacaaaatga ccgttttcaa tctcagattc tagataatca
     1201 atattagtga gatgcagcat ctcatcgcta tggtgacgat accgtctgga aatcatatgc
     1261 ttatgataaa attctttttt attttcatac atccttgcat ctgcctcagc aatcatctca
     1321 ttaatattac ctactgcatt agtccattgt gttcctatcg ctacttgaca aactggtttt
     1381 ttagaaaaac tttcacttaa ttctttaact cgtttttcaa atgactcttt ttttatgctt
     1441 tgacaaataa taataataat aaattcatcc ccaccaatac gataaaaatc agctttttta
     1501 aataccattt t
//
LOCUS       DAOMTA010000825         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4444_length_1510_cov_2.161512, whole genome shotgun sequence.
ACCESSION   DAOMTA010000825 DAOMTA010000000
VERSION     DAOMTA010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_1510_cov_2.161512"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLK68_16065"
     CDS             <1..872
                     /locus_tag="PLK68_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRM91967.1"
                     /translation="STYIIGLQDQVFIDGKIHTIYNQALTQTGRLSSTDPNLQNIPVK
                     TPEGKLIRKAFVPEYDYLVSFDYSQIELRVLAHLAGVKSLIKAFNEDKDIHRHTASEI
                     FRVPEEQVDSTMRRNAKAVNFGIIYGMSDFGLAEQVGVSVGEAREFIKRYFENYPEIK
                     LYMDSNIDFCKKNGYVTTMLNRKRFIREINEKNYMRQEFGKRLAMNSPIQGSAADIIK
                     GAMIKVDELLKKHQLKSKMILQVHDELIFDVYQEELQEVMEIVAKGMTTAIKMDVELK
                     AEGSYAVNWYELK"
     gene            882..>1510
                     /gene="mutM"
                     /locus_tag="PLK68_16070"
     CDS             882..>1510
                     /gene="mutM"
                     /locus_tag="PLK68_16070"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRM91968.1"
                     /translation="MPELPEVETVRRTLKNFVLNKKIISIDVLYPNIIEDDVEEFIEA
                     CTNQTINDIDRAGKFLIFKLDDIAFVSHLRMEGKYHYVEHDEPLNKHDHIIFNLDDNK
                     QLRYNDTRKFGRMKLVSLDNYMNEIPLCKLGPEPFNAKLEDIYPKLHKSNLPIKHAIL
                     DQSIIAGIGNIYANEICFAMGLNPNTPACKLTKKSVQELIEVSSAILNEA"
BASE COUNT          547 a          191 c          290 g          482 t
ORIGIN      
        1 attctacata tattatagga ttacaagatc aagtctttat agatgggaag attcatacaa
       61 tctataatca ggcgttgacg caaacaggaa ggttatcatc aaccgatcct aacctgcaaa
      121 atatacctgt taaaacacca gaaggaaaac tcattcgtaa agcttttgtt ccggaatatg
      181 attatttagt atcttttgat tattcacaaa ttgaacttag agtcttggct catttagcgg
      241 gtgttaaatc attgatcaaa gcttttaacg aagataaaga tattcatcgc catactgcta
      301 gtgaaatatt tagagtacct gaagaacaag ttgatagtac gatgcgacgt aatgctaagg
      361 ctgtaaactt tggaattatt tatggaatgt ctgattttgg tttggcagaa caagttggag
      421 taagcgtagg ggaagcgcgt gaatttatca agcgttattt tgaaaactat cctgagataa
      481 aattatatat ggatagtaat attgattttt gtaagaaaaa tggttatgtt acaacgatgc
      541 tgaatcggaa acgttttatt cgtgaaatta atgaaaaaaa ttatatgcgt caagagtttg
      601 gaaaacgatt agcaatgaat tcaccaatac aaggatcggc agctgatatt attaagggtg
      661 caatgatcaa agttgatgag ctattgaaga aacaccaatt aaaatctaag atgatcttac
      721 aagtacacga tgaattgatc tttgatgttt atcaagaaga attacaagaa gttatggaaa
      781 ttgttgctaa aggaatgacg actgcaatta agatggatgt tgaattaaaa gcagaaggaa
      841 gttatgccgt taattggtat gaacttaaat aggagataat tatgccagaa ctaccagaag
      901 ttgaaactgt tcgtagaacg ttaaaaaatt ttgttttgaa taaaaaaatt atttcaatcg
      961 atgtattata tcctaatatt attgaagatg atgttgagga atttattgaa gcctgtacaa
     1021 atcagactat taatgatatt gatcgggctg gaaaattttt aatctttaaa ttagatgata
     1081 ttgcatttgt ctcacatctt cgtatggaag gaaagtatca ttatgttgaa catgatgagc
     1141 cgttaaataa acatgatcat atcattttca atcttgacga taataagcag ttacgctata
     1201 acgatactcg taaatttggt cgtatgaaat tagttagtct ggataattat atgaatgaaa
     1261 tccccctttg caaattgggt ccagaacctt ttaatgccaa gttggaagat atttatccaa
     1321 aattacataa aagtaattta ccaattaaac atgctatttt ggatcaaagt attattgcgg
     1381 ggattgggaa tatttacgca aatgaaatat gttttgcaat gggtttaaat ccaaatacac
     1441 cggcatgtaa attaacgaaa aagagtgttc aggaacttat tgaggtgtca tcagcaattt
     1501 taaatgaagc
//
LOCUS       DAOMTA010000826         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4450_length_1509_cov_1.931224, whole genome shotgun sequence.
ACCESSION   DAOMTA010000826 DAOMTA010000000
VERSION     DAOMTA010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4450_length_1509_cov_1.931224"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLK68_16075"
     CDS             <1..493
                     /locus_tag="PLK68_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM91969.1"
                     /translation="PLQGLTQGAQPIISFNYGAENIDRVKRAFKLLLKISLSYSMLLW
                     AVAMFIPDTFIYIFTSHGELATYTRWAIRIYMAASGIFGIQIACQQTFIAIGNAKTSV
                     FLAVLRKVLVLIPLIFILPMFIENQAFAVFLAEPIADTIAVSVTATLFYQTYKRLGKE
                     TKA"
     gene            complement(524..640)
                     /locus_tag="PLK68_16080"
     CDS             complement(524..640)
                     /locus_tag="PLK68_16080"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91970.1"
                     /translation="MSFSASLIICVLPFLPGDVLKIFIAAALTNKFKNIIKD"
     assembly_gap    694..703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<704..1097)
                     /locus_tag="PLK68_16085"
     CDS             complement(<704..1097)
                     /locus_tag="PLK68_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRM91971.1"
                     /translation="MNLKVKNMIYCSMFACITAILAQIRFTLPSLVPITLQTLGIYLI
                     GLVLKPKIAFISSLIYILMGAIGLPVYSGFSAGLSTILGPTGGYIFSFPITALIISYL
                     VHYRSTTIFKFGALILGTIICYLIGTLWF"
     gene            complement(1138..>1509)
                     /locus_tag="PLK68_16090"
     CDS             complement(1138..>1509)
                     /locus_tag="PLK68_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRM91972.1"
                     /translation="SDLLKNNTYLKELTTEDYFVRAVEITMDHYHYQSDLLYDLNEVC
                     HEIFNTFKEEYQERYDILEKRFVDIPIKELFSKIKKLSSKDAICAFYHSLNNNEEIET
                     TLISNIFLEEYLIALLLYTLV"
BASE COUNT          519 a          229 c          240 g          511 t
ORIGIN      
        1 accgttacaa gggcttactc aaggagctca gccaattatt agttttaatt atggtgctga
       61 aaatattgat cgagtaaagc gtgcttttaa acttttatta aaaatctcat tatcctattc
      121 aatgctctta tgggcagtag cgatgtttat accagatact tttatttata ttttcacaag
      181 tcatggagaa ctggcgacgt atacacgttg ggcgattcgt atttatatgg ccgcttcagg
      241 aatttttgga attcagattg cttgtcaaca aactttcatt gctattggaa atgctaaaac
      301 atcagtattt ttagccgttc taagaaaggt tttagtttta ataccattga tttttatttt
      361 accaatgttt atagaaaatc aagcatttgc agtattttta gcagagccaa tcgctgatac
      421 gattgcagtt agtgttacag ccacattatt ttatcaaacg tataaacgtt taggaaaaga
      481 aacaaaagca taaaatgaaa aaaagattag gaaagcaaat tccctaatct tttattatat
      541 tcttaaattt atttgttaag gctgctgcga taaagatttt aagtacatca ccaggtaaaa
      601 aaggcaggac gcaaataatt aatgaagcac taaaactcat cttcattata tacataaacc
      661 ggacttccat acttttctgt tatctgttga ggcnnnnnnn nnntaaacca taaagttcca
      721 attaaataac aaattattgt tccgagtatt aaagcgccaa acttaaaaat agtggtactt
      781 cgataatgga caaggtaact gattattaaa gctgtaattg gaaaactaaa tatatagcca
      841 ccagttggtc ctaatatagt acttaaaccg gcactaaagc cgctatagac aggcaggccg
      901 atagctccca ttaaaatata tattaaggaa ctaatgaaag ctattttagg ttttaacacc
      961 agaccaatta aataaatccc gagtgtttgt aaagtgattg ggactagact tggtaaggta
     1021 aatcgaattt gtgccaagat tgcagtgata catgcaaaca ttgaacaata tatcatattt
     1081 tttactttga gattcaaact aattcctcct aggaggattc tagcaaattt tagttaatta
     1141 tacaagcgta tatagtagta aagcgattaa atattcttct aaaaaaatat tactaattaa
     1201 agttgtttcg atttcttcgt tattatttaa tgagtgataa aaagcacaaa tagcatcttt
     1261 actgcttaat ttttttattt tggagaagag ttctttaata ggtatgtcga caaaccgttt
     1321 ttctaaaata tcatagcgtt cctgatattc ttctttaaaa gtgttaaata tttcgtgaca
     1381 cacttcattt agatcataaa gaagatcact ttgatagtga taatgatcca tagtgatttc
     1441 aaccgctcga acaaagtaat cttcggttgt tagttccttt aaataagtat tattctttag
     1501 cagatcact
//
LOCUS       DAOMTA010000827         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4452_length_1509_cov_1.506878, whole genome shotgun sequence.
ACCESSION   DAOMTA010000827 DAOMTA010000000
VERSION     DAOMTA010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4452_length_1509_cov_1.506878"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLK68_16095"
     CDS             <1..408
                     /locus_tag="PLK68_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRM91973.1"
                     /translation="EVVQGNLFEIVPEQPYTSADLNWTNPKSRSSVEMNDKSFRPAVK
                     EKISDMGEYDIIYLGFPIWWYIAPTIINIFLEQYDLRDKTIIPFATSGMSGMGKTNDY
                     LENSCKGARLMKGQRFDENVSADELSDWVKKMK"
     gene            424..981
                     /locus_tag="PLK68_16100"
     CDS             424..981
                     /locus_tag="PLK68_16100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM91974.1"
                     /translation="MQNILVVSGHTDLNNSVANKAILERLENKLPQAEVVYLDKLYSD
                     FQIDVEAEQEKLLNADIIVLQFPIFWYAMPSLLSRWLEETFQHGFSHGSTGDKLKGKK
                     LIASFTTGAPEFMYSYEGAQKYPIEDFLPPIKAMCNLCGLDYFGYVYTGGISYQNRND
                     IEKMAEMKEKAVMHADKLLELLETL"
     assembly_gap    1027..1036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1218..1346
                     /locus_tag="PLK68_16105"
     CDS             1218..1346
                     /locus_tag="PLK68_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91975.1"
                     /translation="MTQKEFGEKLCVSDKIISKWKLSRYPILAKALELSYEELLDG"
BASE COUNT          568 a          183 c          285 g          463 t
ORIGIN      
        1 gaagttgtgc aggggaattt gtttgaaatc gtaccagaac aaccttacac aagtgctgat
       61 ttgaattgga caaatcctaa aagccgttca tcagtagaaa tgaacgataa atcgttcaga
      121 ccagccgtta aagaaaaaat aagtgatatg ggagaatatg acataatcta tttaggattt
      181 cctatctggt ggtatattgc tcctacaatc atcaatattt ttcttgaaca atacgattta
      241 agggataaaa caattattcc atttgcgaca tctggaatga gcggaatggg aaaaacaaac
      301 gattatttag aaaattcatg caagggtgct cgtttgatga aagggcaaag atttgacgaa
      361 aatgttagtg cagatgaatt atctgattgg gttaaaaaaa tgaaataaaa ggagtaatga
      421 cttatgcaaa atatattagt agtatctgga cacacagact taaataattc tgttgcaaat
      481 aaggcaatat tagaaaggct tgaaaataaa ttaccacagg ctgaagttgt ctatttagac
      541 aaactttatt cagattttca aattgatgtt gaagcagagc aagaaaaact tttaaatgca
      601 gatataattg ttttgcaatt tcctattttc tggtatgcta tgccttcatt actaagtcgc
      661 tggcttgaag aaacatttca gcatggcttt tcccacggtt caaccggtga taaattgaaa
      721 ggaaagaaat tgattgcttc ttttacgact ggtgctccag aatttatgta tagttatgaa
      781 ggggcacaga aatatcctat tgaagatttc ctcccgccga taaaggcaat gtgtaatttg
      841 tgtggattgg attactttgg atatgtctat acaggcggaa tatcttatca aaatagaaat
      901 gatattgaaa aaatggctga aatgaaagaa aaagcagtta tgcatgctga taaattattg
      961 gaattgttag aaacactgta acaaaaaaag atcatatttt tgaaaattgg aaattaatca
     1021 gaaaatnnnn nnnnnnagct aaatattctg attagtttta tatataagta attttgtcat
     1081 aaatataaac tctaccgatg gttgattgaa taatcagaat tttaatagta gtataggctc
     1141 aaggagtgaa agatatgagc aatatagata atgaaaaatg cggatccttc atgatggagt
     1201 tatgaaaaga gaaaggtttg acacaaaaag aatttggaga aaaattatgt gtatcagaca
     1261 agataatcag caaatggaag ctatcacgat atccaatact tgcaaaagct ttagaattga
     1321 gttatgagga gttattagat ggttgaattt ggtttcaaca caagaaaaga aaaatattac
     1381 ttatgatagt tgtcaatggc tggcaattct acattttaat tatcgagatc tggtaatttt
     1441 aaaagtacat gtataaattt gtgaaaataa aacatatata ttatttaatc aaagcacaaa
     1501 ttatcttca
//
LOCUS       DAOMTA010000828         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4458_length_1508_cov_2.092223, whole genome shotgun sequence.
ACCESSION   DAOMTA010000828 DAOMTA010000000
VERSION     DAOMTA010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4458_length_1508_cov_2.092223"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..650
                     /locus_tag="PLK68_16110"
     CDS             297..650
                     /locus_tag="PLK68_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91976.1"
                     /translation="MNPFYDENFYYNNFNPQMNPMNQGFPVNRKLKTRANLQKTLHYA
                     SKTVYTINQVIPLVYQIRPIINNTKNAFRVIQAVNHINDIDFDEVEQNITPIDQEKKE
                     TKEESDDVKFENMVQ"
     gene            666..>1508
                     /locus_tag="PLK68_16115"
     CDS             666..>1508
                     /locus_tag="PLK68_16115"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRM91977.1"
                     /translation="MKVYPITPRGYCKGVVRAIELAKKQAHQDDVYILGMIVHNQYIV
                     DALNNLGVKTIDKKGASREELLDQVSQGTVIITAHGASHNVIKKAHSKNIQVINATCP
                     DVIKTHDLIKNYLNQGIEILYIGKTGHPESEGALSIDPAHIHLIENKTDFENLDPNLN
                     YVITNQTTMSLYDVYDLCEYAKTKLNNLIVAKETCQATTVRQEAIAKINDEVDVIFIV
                     GDPHSNNTKKLASIAHEKANKDTYMIESVNDIDISMLKEKHAAAVSSGASTPTYLTNQ
                     VIEYL"
BASE COUNT          594 a          241 c          208 g          465 t
ORIGIN      
        1 cctttctttg ataaattcaa ctaattttaa cataaccaaa ataaaaagca aataaaataa
       61 acctattctt taaatagatt tagtatgttc tattctaagt cctcaaagtt agttttaaag
      121 acctaaatcc cacttaaaaa caaataaaat agttaaacaa caatagttta cccaaacaat
      181 ttttatctag ctattgccct ttgcaaaaat aacctctaac tccaagcaaa ataacaagta
      241 ttcaatatca caattattaa ttatttgcat acattattaa taaaggaggg ataaaaatga
      301 acccattcta tgatgaaaat ttttactaca ataatttcaa tccccaaatg aatcccatga
      361 atcaagggtt tcctgttaat agaaaactaa aaacccgcgc caatttacaa aaaacattac
      421 attatgcctc taaaactgtt tataccatta atcaagtaat tcctctagtc tatcaaatcc
      481 gccctattat caacaatact aaaaatgctt ttagagtcat tcaagcagtt aatcatatta
      541 acgatattga ctttgatgag gttgaacaaa atattactcc tatcgatcaa gagaaaaaag
      601 aaacaaaaga agaatcagat gacgtaaagt ttgaaaatat ggtacaataa ccacgaggtg
      661 atttaatgaa agtatatcca attacaccca gaggatattg taaaggggtt gttcgtgcaa
      721 tcgaattagc taaaaaacaa gcccatcaag atgatgttta tattctaggc atgattgtcc
      781 ataaccaata tattgttgat gctttaaata atttaggtgt caaaacgatt gataaaaaag
      841 gtgcttcgcg tgaagaatta ttagatcagg tttcccaggg aacggttatc attactgctc
      901 atggagctag tcataatgtt ataaaaaagg ctcattcaaa aaatattcaa gtaatcaatg
      961 ctacttgccc cgatgttatt aagactcatg acttaattaa aaattattta aatcaaggga
     1021 ttgaaattct ttatattggc aaaactggtc atccggaatc agagggagca ttatcaattg
     1081 atcctgctca tatccatctg attgaaaata aaaccgattt tgaaaatctt gatcctaatt
     1141 taaactatgt cattacaaat caaacgacaa tgtctttata tgatgtttat gatttatgtg
     1201 aatatgctaa aacaaaacta aataatttaa ttgttgctaa agaaacatgt caagcaacaa
     1261 ctgtacgtca agaagctatt gcaaaaatta atgatgaagt tgatgtcatt tttatcgttg
     1321 gtgatcctca ttcgaacaat actaaaaaat tagctagtat cgcacatgaa aaagctaata
     1381 aggacactta tatgattgaa agtgttaatg atattgatat atctatgtta aaggaaaaac
     1441 atgctgcagc tgttagctca ggggcatcta caccaacata cttgacaaat caagtaattg
     1501 aatattta
//
LOCUS       DAOMTA010000829         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4460_length_1507_cov_2.741047, whole genome shotgun sequence.
ACCESSION   DAOMTA010000829 DAOMTA010000000
VERSION     DAOMTA010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_1507_cov_2.741047"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLK68_16120"
     CDS             <1..245
                     /locus_tag="PLK68_16120"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM91978.1"
                     /translation="TKIIYNPIENVEVTNVTLDQKELSINVGQSKTLVKKLAPLYADE
                     QLTWSSRDEDIASVDQNGIVTAKKVGSIMMKFMLRV"
     gene            266..>1507
                     /locus_tag="PLK68_16125"
     CDS             266..>1507
                     /locus_tag="PLK68_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRM91979.1"
                     /translation="MALNKDVSASTETNQWNKNPTGKLVNDGDYDTRWVSKDGTSITK
                     EQITIDLGEVTTVDNVKLYWESARATDFNIEVSTDGSKYDIVEKLRDEDKNKLTNEIS
                     FDPVEARYVRMQGLTPATKYGYSIYEFEIYNNSDLKLASSVEFKEMSSELYLGENTAL
                     DVVVTPDDATYQDASYTSSNEFVVVCKNNQMIAVGEGTATITANVNGQKITKEITVVK
                     DNARKIAQELTSLTVENGRVDFPTVDGYSFSVFSSDLEKVIAKDGSVNLPVEDQEVAL
                     VVTVSKDGSEDSANTDEIKVMVKGSRDKYELLEKRIKEIEETDWTVYKPGTVKTFKEK
                     LQAVKESLNAGVLLVSEVEKAGENLEAAFKGLEIKSDKKTLDALIKELEELDTKVYTE
                     VSVARVEKALEKAETVSENEDA"
BASE COUNT          588 a          191 c          321 g          407 t
ORIGIN      
        1 aaacaaagat tatttataat ccaattgaaa atgttgaagt aacaaatgtt actttagatc
       61 aaaaagagtt atctattaat gtaggacaaa gcaaaacttt agttaaaaaa ttagcaccat
      121 tatatgcaga tgagcaatta acttggtcaa gccgtgacga agatattgca agtgtagatc
      181 aaaacggtat cgttactgct aaaaaggtag ggagtataat gatgaaattt atgctacgag
      241 tgtgattaat gttcttggtg aaaatatggc attaaataaa gatgttagtg cttcaacaga
      301 aacaaatcaa tggaataaaa atccaacagg taaactggtt aatgatggtg attatgatac
      361 acgctgggtt tctaaagatg ggacatcaat cactaaagaa caaattacaa ttgatttagg
      421 tgaagtgaca actgttgata atgttaaact ttattgggaa tcagcaagag caacagattt
      481 taatattgaa gtgtctactg atggttcaaa atatgatata gttgaaaaat tacgtgatga
      541 ggataaaaat aaattaacta atgagatttc atttgatccg gttgaagcaa gatatgtaag
      601 aatgcaggga ttaacaccag caactaagta tggttattca atctatgaat ttgaaattta
      661 taataattca gatttaaaat tagcaagtag tgttgaattt aaagaaatgt catcagaact
      721 gtatcttggt gaaaatacag cacttgatgt agttgtaact cctgatgatg caacatatca
      781 ggatgcttca tatacttcaa gcaatgagtt cgttgttgtt tgtaaaaaca atcagatgat
      841 tgctgttggt gaaggaacag caacaattac tgccaatgtc aacggtcaaa aaataactaa
      901 agaaattact gttgttaaag ataatgcccg taagattgct caggagctga catcattaac
      961 tgttgaaaat ggtcgtgtgg acttcccgac agttgacggc tacagcttca gtgtattcag
     1021 ttctgatctg gaaaaagtta ttgccaaaga cggaagtgtt aacttaccgg ttgaagatca
     1081 agaagtggca cttgttgtaa ctgtatctaa agacggcagt gaagacagtg caaatactga
     1141 tgaaatcaaa gtgatggtta aaggtagcag agataaatat gagctgttgg aaaaacgaat
     1201 caaagaaatc gaagaaacag actggacagt atataaacca ggtacagtta aaacatttaa
     1261 agagaaattg caggcagtca aggaaagtct aaatgcagga gtactgttag tcagtgaagt
     1321 tgaaaaagca ggagaaaatc ttgaagcagc atttaaggga ttagagatca aaagtgataa
     1381 aaagacactt gatgcgctga ttaaagaact agaagaactt gataccaaag tatataccga
     1441 agtatcagtg gcaagagtgg aaaaagcatt agaaaaagca gagacagtca gtgaaaacga
     1501 agatgcc
//
LOCUS       DAOMTA010000830         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4464_length_1507_cov_2.099174, whole genome shotgun sequence.
ACCESSION   DAOMTA010000830 DAOMTA010000000
VERSION     DAOMTA010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_1507_cov_2.099174"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLK68_16130"
     CDS             complement(<1..239)
                     /locus_tag="PLK68_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91980.1"
                     /translation="MKETYSKNYFEKYAALTLTKVLDVSADNIIQADRPDLRIPKRNF
                     GIEVTQALTPAEAVADIKKPLYSTLKLNPFDHDEK"
     gene            438..>1507
                     /locus_tag="PLK68_16135"
     CDS             438..>1507
                     /locus_tag="PLK68_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRM91981.1"
                     /translation="MVDYSIKKYNRKIVYQLIYQAKQITKQEIAERLKLSLPTVSSNL
                     LELEQADLIKKEDINESTGGRKPLAISIKEQSRIAIGVSILKEGITFVALDLYAQLIR
                     KQTVALVYDNSESYFKQYGQALNEFIDCLQIKKENFLGITIAIQGIVSQDGKKVIYGP
                     ILNNQGLTLEKFEKSSDLPVKMIHDSHAAGLAELNFSEDNNDAIYLSLNHNLGSAVFM
                     AGKVFQGNNYSSTFEHMTLVENGRQCYCGKKGCFEAYCNEDALLKNHTNMTLEEFYKQ
                     LRQKDQTAMQSWNDYFHYLALAIHNLTMVLDAPVIIGGKIASLFENEDLDLLTKLVSD
                     LDPLKFTVPDIKIGQCLKETAA"
BASE COUNT          524 a          174 c          266 g          543 t
ORIGIN      
        1 tttttttcat catggtcgaa aggatttaat tttaaagtac tatataaagg tttttttatg
       61 tcagcaactg cttcagctgg agttaatgct tgagttactt caattccaaa attacgtttt
      121 ggaatgcgaa ggtcaggacg atcagcttga ataatattat cagctgatac atctaatact
      181 tttgttaggg ttaatgcagc atatttttca aaataattct tactatatgt ctctttcatt
      241 aattgacctc gatttttaaa aagtgataat cattcattga ttatcactta gtgggtttta
      301 tactgtcttc tttattcata ttatagcatg gtttatttta ttttcaaagt aagtaataag
      361 gatatttgga tattcattgc gatatattta gataactgtt atacttttat aaaggttttt
      421 tagataaggt ggtatttatg gttgattata gtattaaaaa gtataatcgt aagattgtat
      481 atcagctgat atatcaagct aagcaaatta caaaacagga aattgcggaa agattaaaat
      541 tatctttgcc cactgtttct agtaatctct tggaattaga acaggctgat ttgataaaaa
      601 aagaagatat taatgagtct acggggggac gtaagccgtt agcaatctct attaaggaac
      661 aatctagaat tgcaattggt gtcagtattt taaaagaagg aataactttt gttgctttag
      721 atttgtatgc tcaactgatt cgtaaacaaa cagttgcttt agtttatgat aatagtgaaa
      781 gttattttaa acaatatgga caggcattaa atgaatttat cgattgttta caaattaaaa
      841 aagaaaattt tttagggatc actatagcta ttcaggggat cgtatctcag gatggaaaaa
      901 aggttattta tggtccaatt ttaaataatc agggattaac gttagaaaag tttgaaaaat
      961 ctagtgattt accagttaaa atgattcatg attctcatgc tgctggactt gctgaattaa
     1021 atttcagtga agataataac gatgctattt atctatcttt aaatcataat ctaggtagtg
     1081 ctgtatttat ggctggaaag gttttccagg gaaataatta cagttcgaca tttgagcata
     1141 tgacattagt agagaacgga cgtcagtgtt attgtggtaa aaaagggtgt tttgaagctt
     1201 attgtaatga agatgcttta ttaaaaaatc atacaaatat gacattagaa gaattttata
     1261 aacaattacg gcaaaaagac caaactgcaa tgcaatcatg gaatgattat tttcattatc
     1321 tagcattagc aattcataat ttaaccatgg ttttagatgc accagtaatt attggtggta
     1381 aaatagcttc attgtttgaa aatgaagatc ttgatctatt aacaaaatta gttagtgatt
     1441 tggatccatt aaagtttact gttccagata ttaaaatagg tcaatgttta aaagaaacag
     1501 cagcaat
//
LOCUS       DAOMTA010000831         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4467_length_1506_cov_3.652653, whole genome shotgun sequence.
ACCESSION   DAOMTA010000831 DAOMTA010000000
VERSION     DAOMTA010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4467_length_1506_cov_3.652653"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /gene="metG"
                     /locus_tag="PLK68_16140"
     CDS             complement(<1..1459)
                     /gene="metG"
                     /locus_tag="PLK68_16140"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRM91982.1"
                     /translation="MKNEKDYYLTTAITYTSGKPHIGNTYEIILADAIARFKRQEGYN
                     VYFQTGTDEHGQKIELKAKEAGIPPKQFVDEKAGEVKRIWDLMDTTYDRFMRTTDDYH
                     KSQVQKIFKKLYDQGDIYLGHYEGKYCTACESFFTESQLVDGKCPDCGGEVHDAKEEA
                     YFFKLSKYADRLIEHIETHPEFIQPVSRKNEMMNNFLKPGLQDLCVSRTSFTWSIPVD
                     FDPKHVVYVWIDALSNYITGLGYDVDGNHGELYQKYWPADLHLIGKDIVRFHTIYWPI
                     MLMALDIPLPKQVFGHPWLLQGESKMSKSKGNVIYADDLVDIFGVDAVRYYVLHEIPY
                     DNDGSITWDLLVERINSDLANVLGNLVNRTIAMSNKYFGGIVNNPKVTEAVDDELIEI
                     ALAMPKKVIGKMDEFKSSDALDAIFTLLRRTNKYIDETMPWALAKDETKRDRLATVLY
                     NLIESIRFAAIALIPYMPSTANKILDQINTDQRDLL"
BASE COUNT          477 a          281 c          191 g          557 t
ORIGIN      
        1 ctaataaatc tctttgatca gtattaattt gatctaaaat cttattagca gtagatggca
       61 tatatggaat taacgcaatc gctgcaaaac gaatactttc aattaagtta taaagaactg
      121 tcgctaaacg atcccttttt gtttcatctt tagcaagcgc ccatggcatt gtttcatcaa
      181 tatatttatt agttcttctt aataatgtaa agatggcatc aagggcatca gatgacttaa
      241 attcatccat cttaccaatt acttttttag gcattgctaa tgctatttca attaactcat
      301 catcaactgc ttctgtaact tttgggttat taacaattcc gccaaagtat ttatttgaca
      361 tagcaatagt acgatttact aaatttccta agacattagc taaatcagaa ttaattcttt
      421 ctactaaaag atcccaggtg atgcttccat cattatcata tggaatttca tgtaaaacat
      481 aatatctaac tgcatctaca ccaaaaatgt ctactaaatc atctgcatag attacatttc
      541 cttttgattt tgacatttta ctttcacctt gtaataacca tggatgtcca aatacttgtt
      601 taggtaaagg aatatctaaa gccattagca taattggcca ataaatagta tggaaacgca
      661 caatatcttt accaattaaa tgtagatctg caggccaata cttttgatat aattcgccat
      721 gattaccatc gacatcatac cctaatcctg taatatagtt acttaatgca tcaatccata
      781 cataaacaac atgtttagga tcaaaatcta cgggaatcga ccaagtaaat gaggttcttg
      841 atacgcataa atcttgtaat cctggtttta aaaaattatt catcatttca ttctttcgtg
      901 atactggttg aataaattca gggtgtgttt caatatgttc aattaaacga tcagcatatt
      961 ttgataattt aaagaaatat gcttcttcct tagcatcatg gacttcacct ccacagtctg
     1021 gacattttcc atctactagt tgtgattctg taaaaaatga ttcacaagcc gtacaatatt
     1081 taccttcata atgacctaag taaatatccc cttgatcata taattttttg aagatttttt
     1141 gtacttgtga cttatgataa tcatcagtcg ttctcataaa tcgatcatat gtagtatcca
     1201 ttaaatccca aattcttttt acttcaccag ctttttcatc aacaaactgc tttggcggga
     1261 ttcctgcttc tttagctttt aactcaattt tttgaccatg ttcatctgtc ccagtttgga
     1321 aataaacatt atatccttct tgtcttttaa atcgagcaat tgcatctgct aaaataattt
     1381 cataagtatt tccaatatga ggttttcctg aagtatatgt aattgcagtt gttaaataat
     1441 aatctttttc attcttcata tatatttctc ctttatctca tcttgtttaa gacctccccg
     1501 ggtctt
//
LOCUS       DAOMTA010000832         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4469_length_1506_cov_2.507926, whole genome shotgun sequence.
ACCESSION   DAOMTA010000832 DAOMTA010000000
VERSION     DAOMTA010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4469_length_1506_cov_2.507926"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1213..>1506)
                     /locus_tag="PLK68_16145"
     CDS             complement(1213..>1506)
                     /locus_tag="PLK68_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM91983.1"
                     /translation="RMKKRVIIPMLVFAVVFSNVYASNNNHRSKRVNTVKTVNAAARK
                     AVKSIKSVKAVNTLNEVNRDTISPDSSSEIRGKLLIFYLDKAKPYKPEDGPAL"
BASE COUNT          509 a          263 c          233 g          501 t
ORIGIN      
        1 taaaaattta gaatacacat tataaacaag attataaaaa tgcttcttgt caagcactcc
       61 aaagttcggt aatataatga atttatcgtc tctcataata ccttttatca cctcatcctc
      121 aaaaagtttt gtgataaggc ctttgaatag ttctactttg ttttcgtcgg gtggtagaaa
      181 aacatgccat agcatcataa agtgattaac acattcttcg tttgttagaa ctttggtttg
      241 attatttcct ataggatcca ttgtatgttt aagttttata aatgcaaagg tacaaaaaaa
      301 tcataaacaa tacaaatatt acatgcataa attaatgtac aaaatacaat catcttatct
      361 acgtatagca tttaatttac aatatttaat gcgaatttat taaaatgaaa ttacattaca
      421 tacataagta ggtcttgata cgtacgctgg catatgtaca tatcatataa atatgtaacg
      481 aataaggcgt agacaggatt tcacccttga tggttactct taactaacac aaacacatca
      541 tcaagtatac gccctagttg tgggttcaaa ctcaactcat tatttgtgtc tatctaaata
      601 tcaaaggtgt ctgagctaat tttgaataac gcaaatactt acgtaaatgc aagtacaaag
      661 gtaataaatt atcagttatt atcacttatg taaataaata tcagatgcat ttaatcacat
      721 gcatttgctg tttattcatc aaataaggtg accatattta ccacataagc agtattactg
      781 atcacataag gcggtcgtat taacgatcac atcaataagt agtatattat tcctccctct
      841 gcggttaggg aggtgtcacg aagtgacgga ggatacgaaa aacaacgtcc caaccgcttt
      901 aataaagaca tcttagacat caattaggca aaattgacga agcgctgtat cggtgccaag
      961 cgtagcgctg taatcggtac catgctatca ctgtaaacag taccgcttgg tatcggtgca
     1021 aactgtacca agttttccag ttttgtcttg tttcgtgcaa agataagagg ttaaacttaa
     1081 ttggcagaac caaaatgaac tttggtaccg gttccagtga tatcatggta ccaattgctg
     1141 tgatatgata tatgattgtg gaactttata ttaaaaaaat agaggcaagc tatcaactca
     1201 cctctttgtt ttctacaatg ctggaccatc ctccggttta taaggtttgg ctttatctaa
     1261 ataaaaaatt aaaagttttc cccttatttc cgaggaagaa tctggagaga ttgtatctct
     1321 attaacttca tttagtgtat taacagcttt aactgattta attgacttaa ctgcttttct
     1381 tgccgctgca ttaactgtct taacagtatt cactctttta gacctatggt tattattact
     1441 tgcataaaca ttgctaaata caactgcaaa aaccaacata ggaataatta ctcttttctt
     1501 cattct
//
LOCUS       DAOMTA010000833         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4470_length_1506_cov_2.024810, whole genome shotgun sequence.
ACCESSION   DAOMTA010000833 DAOMTA010000000
VERSION     DAOMTA010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_1506_cov_2.024810"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..639
                     /locus_tag="PLK68_16150"
     CDS             31..639
                     /locus_tag="PLK68_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HRM91984.1"
                     /translation="MAGDQGLVTLECALAMNTYEQIDFLEIKEKGSRKIINHTVFKES
                     EFDKVVKLYDEVIVATGSNDLREEKIKMLLAFGIPLATIVHPTALISSSAKIGSGTTI
                     LANVIININTKVGIGCIVNNGAIIEHDCMVGNYVNICPKFAMAGHSSIGYKSYLGIGS
                     TVIDDIRIGNRVTVGAGAVVVSNISDNIVAIGVPAKKMLVDK"
     gene            873..>989
                     /locus_tag="PLK68_16155"
     CDS             873..>989
                     /locus_tag="PLK68_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRM91985.1"
                     /translation="MIYLDYVATTPLNSEVLNTYHNLLSNYFYNADSMYDKGI"
     assembly_gap    990..999
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1000..>1506
                     /locus_tag="PLK68_16160"
     CDS             <1000..>1506
                     /locus_tag="PLK68_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM91986.1"
                     /translation="RLMEHSRKLISESFKVKEDEIIFTSCGSEANNMAIKGTAFQYQN
                     RGKHIITTAIEHSSVYETCKELEKFFGFEVTYLGVDHKGRISLEELEKSIRDDTILVS
                     IMYVNNEIGVINPIDEIKKIVKKYDKVKLHFDMVQALGKLPIDLNDVDLASFSAHKIY
                     GLKGSGLL"
BASE COUNT          523 a          148 c          298 g          527 t
ORIGIN      
        1 ccatcatgtg atcctgcaag gcaatctgac atggcaggag atcaaggact tgtgactctt
       61 gagtgtgctt tagccatgaa tacatatgaa caaattgatt ttttagaaat caaagaaaaa
      121 ggaagtagaa aaataattaa tcatactgta tttaaagaaa gtgaattcga taaagtagta
      181 aaattatatg atgaggttat tgttgcaact ggtagcaatg atttacgaga agaaaaaata
      241 aaaatgttac ttgcttttgg tattccttta gcaacgatag tccatccaac agctttaatt
      301 agttcatctg caaagattgg tagtggtaca acgatattgg ctaatgtaat aattaatatt
      361 aatacaaagg tcgggattgg ctgtatagtc aataatggag caattattga acacgattgt
      421 atggtaggaa attatgttaa tatttgtcct aagtttgcga tggcaggtca cagttcgatt
      481 ggatataaat cttatctggg aataggtagt actgtcattg atgatattag aattggtaat
      541 cgggtaacag ttggtgctgg agcggtagtt gtttcaaaca tttctgataa tatcgttgcg
      601 attggtgttc cagctaagaa aatgctggtg gataagtaaa aagtgttgtg ttaaaatatt
      661 taagataatt acaatttgat ggtttacgtt ggttttataa aagtaattat ataggaaaat
      721 taaagtactt tggaattgca agtaagaaaa tgattttatt tctttacttt cgtagtaaat
      781 attgtagaat tatttaatgt tgtttgaaag atattttggt ggagtcataa gactttggat
      841 ttttattgca tataataatg tagggggtta cgatgatata tttagattat gtggcaacaa
      901 cgccattaaa ttcagaagtg ttaaatactt atcataattt attatccaat tatttttata
      961 atgctgattc aatgtatgat aaaggtattn nnnnnnnnna tcgtttgatg gaacattcac
     1021 gtaaattaat tagtgagtcc ttcaaagtta aagaagatga aattattttt actagctgtg
     1081 gtagtgaagc taataatatg gcaattaaag gtacagcctt tcaatatcaa aatcgtggta
     1141 aacatattat tactacagct attgaacatt catctgttta tgaaacttgt aaagagttag
     1201 aaaagttttt tggatttgag gtaacttatt taggggttga tcataaaggg cggatttctt
     1261 tagaagaatt agaaaaaagt attcgtgatg atacgatttt agtatcaatt atgtatgtaa
     1321 ataatgaaat tggggtcatt aatcctattg atgaaattaa aaaaattgtt aaaaaatacg
     1381 ataaggtaaa actgcatttt gatatggtac aggctttagg aaaattacca atcgatttaa
     1441 atgatgtgga tttagcttct ttttcggcac ataaaattta tggtttaaaa ggtagtggct
     1501 tattat
//
LOCUS       DAOMTA010000834         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4478_length_1504_cov_2.307798, whole genome shotgun sequence.
ACCESSION   DAOMTA010000834 DAOMTA010000000
VERSION     DAOMTA010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_1504_cov_2.307798"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1504)
                     /locus_tag="PLK68_16165"
     CDS             complement(<1..>1504)
                     /locus_tag="PLK68_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRM91987.1"
                     /translation="LDNNLMLISAYLGSPQIELNEGDSEKEIPHLILIDLEKMEVKLD
                     IPVEQYNLDYSRAVKGIFDNQPALVVPTYASTGKLRLVAYSLNDGTVLKQTNLNLNGI
                     NSKNVKIHFANQGEHYLLQVDGGVSLLVDQDLKTIEYLGSVKVVNKVVDLSDGTIVSV
                     LNNGKITQIKKMGLGGRDDNLVTFNIPDGYHNNGFEAINYDEKHNHLLSLVSELNANG
                     EVIASHIVIMNLNDGNTLADKKVLLEKGYDENNKYYEHYLIGETIRYFTDMNNDGKSE
                     IIVDENILDGASLTFRSVYNKSFEGATTIIDVGDVNNDGISDLVNIGESEMRLYYSLK
                     NGYDITYQKTNIAKSYDKKLQNNIHVKVLGDLDHDGIKDFVINAYNDKGCQCFQVIKG
                     KDLSVRYSLLKDGVVYENEEDSFSVTGIDYDNDGVDDLVYGIYDEMRTVLSGATGEKL
                     FEYLITDYHNDWDNSMGDPVPLENIVSFNLIENGNSVVKINDLNDDGIQE"
BASE COUNT          519 a          308 c          134 g          543 t
ORIGIN      
        1 aactcttgaa tcccgtcatc attaagatca ttgattttaa ctacactatt tccattttca
       61 attaaattaa aactaactat attttccaaa ggaaccggat cacccataga attatcccaa
      121 tcattatgat aatcagtaat taagtattca aataactttt caccagttgc tccactaaga
      181 acagtcctca tttcatcata aataccataa acaagatcat caactccatc attgtcataa
      241 tcaattcctg tcacactaaa actatcttct tcattttcat agacaacacc atcttttaat
      301 aatgaatatc gtaccgataa gtctttccct ttaatgactt ggaaacattg acagccttta
      361 tcattataag cattgattac aaaatctttg attccatcat gatcaagatc gcctagcact
      421 ttaacatgaa tattgttttg caatttttta tcataacttt tagcaatatt agttttttga
      481 taagttatat cataaccatt ctttaacgaa taatataatc gcatttcact ttcaccaata
      541 ttgactagat cacttattcc atcattattc acatcaccta cgtcaataat cgtagtagca
      601 ccttcaaatg atttattata aacacttcta aatgttaacg atgccccatc aagaatattc
      661 tcatcaacaa taatttctga tttgccatca ttattcatat ctgtaaaata tctaattgtt
      721 tcaccaatta aataatgttc ataatactta ttattttcat catagccttt ttctaataat
      781 acctttttat ctgccagcgt atttccgtca tttaaattca taattacgat atgactagca
      841 attacctcac cattagcgtt tagttcactg actaaactta aaagatgatt atgtttttca
      901 tcataattaa tcgcttcaaa accattatta tgataaccat ctggaatgtt aaacgtaact
      961 aaattatcat cacggccacc taaacccatt tttttaattt gcgttatttt accattattc
     1021 aaaacagaca cgattgtacc atcactaaga tcaacaactt tattaacaac tttaacacta
     1081 cctaagtact ctattgtttt caaatcttga tcaacaagca gtgacacacc cccgtcaact
     1141 tgtaataaat aatgttctcc ttgattagca aaatgaattt tcacattttt actattaata
     1201 ccattaagat tcaaattagt ttgttttaat actgtaccat catttaatga ataagcgaca
     1261 agtcttaact ttccagtact agcatacgtt ggaacaacca aagcaggctg attatcaaaa
     1321 atacctttaa ccgctctact ataatctaaa ttatattgct caacaggaat atccagttta
     1381 acctccatct tttcaagatc aataagtatt aaatgaggta tttctttttc actatctcct
     1441 tcatttaatt caatttgtgg actccccaag taagcactta tcaacattaa attattatct
     1501 aata
//
LOCUS       DAOMTA010000835         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4485_length_1502_cov_2.356600, whole genome shotgun sequence.
ACCESSION   DAOMTA010000835 DAOMTA010000000
VERSION     DAOMTA010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4485_length_1502_cov_2.356600"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="rimM"
                     /locus_tag="PLK68_16170"
     CDS             <1..478
                     /gene="rimM"
                     /locus_tag="PLK68_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRM91988.1"
                     /translation="THGLKGELKIRSNSDFADKRFKKGNEIIISYQNQDLVYKIITSR
                     IHKGNYLVSFRDNQDINLVEKYIGSFVYGYKDDELLDADEYFYTDLIGMQVVSTEGTK
                     IGPVTSIYDNTRHDILNIDHNGKNVAIPYVDAFIKDVDVEKKIIVVMLIKGLIDED"
     gene            468..1187
                     /gene="trmD"
                     /locus_tag="PLK68_16175"
     CDS             468..1187
                     /gene="trmD"
                     /locus_tag="PLK68_16175"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRM91989.1"
                     /translation="MKIDILTLFPEMFTGFLNTSIIKRAIDKGLVTIELHDFREFSID
                     KHKHVDDYPYGGGQGMVLMCAPIVECLKTIATDDSFIILMSPQGITLNHGCAVELSAK
                     KHLIIICGHYEGFDERIRDYVDMELSIGDYVLTGGELGSMVVSDAVIRLLDGAIKEDS
                     HMDDSFSHGLLEYPQYTRPQCYDGNEVPEVLMSGHHENIRKWRKFQSLKKTYLKRPDL
                     LESYQFDAESIEMMKKIKEND"
     gene            1294..>1502
                     /locus_tag="PLK68_16180"
     CDS             1294..>1502
                     /locus_tag="PLK68_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRM91990.1"
                     /translation="MNLQLVEQITKKQMRNDIPEFKAGDTLKVFVKIKEGEKFRIQLF
                     EGVCIARKGSGISESFTVRKISYQVG"
BASE COUNT          538 a          170 c          299 g          495 t
ORIGIN      
        1 aacacatggc ttaaagggag aactaaaaat tagatcaaat agtgattttg ctgataaaag
       61 atttaaaaaa ggaaacgaaa ttattataag ttatcaaaat caagatttgg tctataaaat
      121 tattacatcg cggatacaca aaggaaatta tttagtatca tttagggata atcaagatat
      181 taatttggtt gaaaaatata ttggttcttt tgtttatggg tataaagatg atgaactctt
      241 agatgctgat gaatattttt atacagactt aattggaatg caggttgttt ctacagaggg
      301 aacaaaaatt ggtccagtta cttcaattta tgataatact cgtcatgata ttttaaatat
      361 tgatcataat ggtaaaaatg ttgcaatacc gtatgttgat gcatttatta aagatgttga
      421 tgtagaaaag aaaattattg tagttatgct aataaagggg cttattgatg aagattgata
      481 ttttaacatt gtttccagaa atgtttacag gttttttaaa tacgtcgatc atcaaaagag
      541 cgattgacaa aggtttagtt actattgaat tacatgattt tagagagttt tctattgata
      601 aacataagca tgtcgatgat tatccttatg gcggaggaca agggatggtt ttaatgtgtg
      661 caccaattgt tgaatgtctc aaaacaattg caactgatga tagttttatt atcttaatgt
      721 cacctcaagg cattactttg aatcatggtt gtgctgtaga attgtcagct aaaaaacatt
      781 taattatcat ttgtggtcat tatgaaggtt ttgatgaacg aatccgtgat tatgttgata
      841 tggaattatc gattggtgat tatgttttaa ctggtggtga actaggaagt atggttgtta
      901 gtgatgcagt gatacgctta ttagatggag caatcaaaga agacagtcat atggatgatt
      961 cttttagcca tggactgctt gagtatcctc agtatactcg cccacaatgc tatgatggta
     1021 atgaagtacc tgaggtacta atgagtgggc atcatgaaaa tattcgcaaa tggcgcaagt
     1081 tccaatcatt aaaaaagacc tatttgaaac gaccggactt attggaatcg tatcaatttg
     1141 atgctgaaag tatcgaaatg atgaaaaaaa taaaagaaaa tgattgattt ttatgattga
     1201 tcagtctata atgtaacagt cgcaagactg cagatgttcc gctggttaat cgaatgaacg
     1261 tttaagacaa aattttgata aggagaaata gtaatgaatt tacaattagt agaacaaatc
     1321 actaaaaaac aaatgagaaa tgatattcct gagttcaaag caggggatac attaaaagtt
     1381 ttcgtaaaaa ttaaagaagg tgaaaaattc cgtattcagt tatttgaagg agtttgtatc
     1441 gccagaaaag gtagtggaat ttctgaatct tttacagtaa gaaaaatctc ttaccaagtt
     1501 gg
//
LOCUS       DAOMTA010000836         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thomasclavelia ramosa isolate AMR_MDS_5768
            NODE_4495_length_1500_cov_2.123875, whole genome shotgun sequence.
ACCESSION   DAOMTA010000836 DAOMTA010000000
VERSION     DAOMTA010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867989
            Sequence Read Archive: SRR1616986
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thomasclavelia ramosa (wastewater metagenome)
  ORGANISM  Thomasclavelia ramosa
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Coprobacillaceae; Thomasclavelia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.169
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:10:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,238
            CDSs (total)                      :: 3,215
            Genes (coding)                    :: 3,172
            CDSs (with protein)               :: 3,172
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 25 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 6 of 43
            Pseudo Genes (multiple problems)  :: 4 of 43
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Thomasclavelia ramosa"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_1500_cov_2.123875"
                     /isolate="AMR_MDS_5768"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1547"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-06-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLK68_16185"
     CDS             <1..644
                     /locus_tag="PLK68_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM91991.1"
                     /translation="ILLVPFTSIIVAGIVTIFIGPYITKLIVWIGDLINQGVNMQPFF
                     MSIVVAVLMGMALTAPISSAAIGVMLGLSGLAAGAALAGCCAQMIGFAVMSMDDNDIG
                     DVVAIGIGTSMLQFKNIVKHPIIWLPPILTSAIIAPISTCLLEISCSAIGSGMGTAGL
                     VGILEAVNVMGNNYWIPIIVIDLLAPMLISFGIYKAFRKLNYIKAGYLKLDRF"
     gene            748..>1500
                     /locus_tag="PLK68_16190"
     CDS             748..>1500
                     /locus_tag="PLK68_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM91992.1"
                     /translation="MLFTYKQIANLNETETFIYRYVMKHIKVVTNMSVRDLAECTHVS
                     TATVVRFCQKLDCNGFVEFKTKLKLFNDGLTLPETDDEIDVLLEFFNYARSVDFKEKI
                     NKFVKYIKEAKSICFLGIGTSGTLGKYGARYFSNVGYYSQSIDDPYYPPPVDGNESSL
                     LIALTESGETREVIDQLKMYQSQNTKIVVITNKPGSTIDQMADLGIYYYVKDIILPQT
                     YNISSQVPVLYILERVTHELQNAKEKSLPLKFT"
BASE COUNT          479 a          187 c          309 g          525 t
ORIGIN      
        1 atattttgtt agtgcctttt acttctatta tagttgcggg aatagtgaca atatttattg
       61 gtccatatat cactaaatta attgtttgga ttggtgattt aattaatcaa ggggtaaaca
      121 tgcagccatt tttcatgtca attgtagttg cagttttgat gggaatggct ttaacagcac
      181 cgatatctag tgcggcaatc ggagtgatgt tgggacttag tggtttagct gctggggctg
      241 ctttagctgg gtgttgtgct caaatgattg gttttgcggt aatgtcgatg gatgataatg
      301 atattggtga tgttgtagca attggcattg gaacaagtat gctgcagttt aaaaatattg
      361 taaagcatcc gataatttgg cttccgccga ttttaacaag tgctattatt gctccaattt
      421 ctacttgttt gttggaaata tcttgttcag caattggttc aggaatggga actgcaggac
      481 tggtagggat tttagaagct gttaatgtga tgggaaataa ttattggatt ccaatcattg
      541 ttattgattt actagcacca atgttgatca gctttggaat ttataaagct tttagaaaat
      601 taaactatat taaagcaggg tatcttaaat tagaccgttt ttagttaata aataaatttt
      661 aaactcttaa atttggtgga tatatgtgtt agaattatac ataatcaggg aagaaagctt
      721 catccaacaa aaaaaggggg cgataaaatg ctgtttacat acaagcaaat tgctaatttg
      781 aatgaaacag aaacatttat ttatcgttat gtaatgaagc acattaaagt tgttacaaat
      841 atgagtgttc gtgatctggc tgaatgtact catgtatcaa cggcgacagt agtacggttt
      901 tgtcaaaaat tggattgtaa tggttttgta gaattcaaga caaagttaaa gttatttaat
      961 gatggactta ctctaccaga aactgatgat gaaattgatg ttcttttaga attttttaac
     1021 tatgcacgaa gtgtagattt taaggagaaa attaataaat ttgttaagta tatcaaggaa
     1081 gctaagtcga tttgtttttt aggaattgga acaagtggta ctttaggaaa atatggggct
     1141 cgttattttt caaatgtagg gtattattca caaagtatcg atgatcctta ttatccccca
     1201 ccagttgatg gtaatgaaag ttcattatta attgctttga ccgaaagtgg tgaaactcga
     1261 gaagtaattg atcagcttaa aatgtatcag agtcagaata caaaaattgt ggtaatcact
     1321 aataagccag gatcaacaat tgatcaaatg gcagatttgg gaatctacta ttatgttaaa
     1381 gatattattt taccacaaac atataatatt tcatctcaag ttcctgttct ttatattctt
     1441 gagcgtgtta ctcatgagtt acaaaatgct aaggaaaaat cgctgccgtt gaagtttacc
//
